LOCUS       DADMFL010000001        86235 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.001, whole genome shotgun sequence.
ACCESSION   DADMFL010000001 DADMFL010000000
VERSION     DADMFL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.001"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..1592)
                     /locus_tag="JNZ27_000001"
     CDS             complement(<1..1592)
                     /locus_tag="JNZ27_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8662492.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JNZ27_000002"
     CDS             complement(1576..1932)
                     /locus_tag="JNZ27_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8662493.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="JNZ27_000003"
     CDS             complement(2055..2237)
                     /locus_tag="JNZ27_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662494.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JNZ27_000004"
     CDS             complement(2221..2661)
                     /locus_tag="JNZ27_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8662495.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JNZ27_000005"
     CDS             complement(2661..2957)
                     /locus_tag="JNZ27_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8662496.1"
                     /translation="MTEELITLEEEKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JNZ27_000006"
     CDS             complement(2954..3292)
                     /locus_tag="JNZ27_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8662497.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JNZ27_000007"
     CDS             complement(3289..4500)
                     /locus_tag="JNZ27_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8662498.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JNZ27_000008"
     CDS             complement(4502..5074)
                     /locus_tag="JNZ27_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY8662499.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JNZ27_000009"
     CDS             complement(5114..6271)
                     /locus_tag="JNZ27_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8662500.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6571..6873)
                     /locus_tag="JNZ27_000010"
     CDS             complement(6571..6873)
                     /locus_tag="JNZ27_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662501.1"
                     /translation="MEHYWLFVTVRHSYYLKQTDTTEENAMPMKFDEILKQRDKYHAD
                     NMETMNITDYRAFLETGALIEKDHHGFVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6937..7098)
                     /locus_tag="JNZ27_000011"
     CDS             complement(6937..7098)
                     /locus_tag="JNZ27_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY8662502.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7108..7413)
                     /locus_tag="JNZ27_000012"
     CDS             complement(7108..7413)
                     /locus_tag="JNZ27_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662503.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATLGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7410..7688)
                     /locus_tag="JNZ27_000013"
     CDS             complement(7410..7688)
                     /locus_tag="JNZ27_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662504.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7968..9716)
                     /locus_tag="JNZ27_000014"
     CDS             complement(7968..9716)
                     /locus_tag="JNZ27_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8662505.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9713..10012)
                     /locus_tag="JNZ27_000015"
     CDS             complement(9713..10012)
                     /locus_tag="JNZ27_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY8662506.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10018..10251)
                     /locus_tag="JNZ27_000016"
     CDS             complement(10018..10251)
                     /locus_tag="JNZ27_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662507.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10253..10473)
                     /locus_tag="JNZ27_000017"
                     /pseudo
     CDS             complement(10253..10473)
                     /locus_tag="JNZ27_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10466..10687)
                     /locus_tag="JNZ27_000018"
     CDS             complement(10466..10687)
                     /locus_tag="JNZ27_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662508.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10677..10877)
                     /locus_tag="JNZ27_000019"
     CDS             complement(10677..10877)
                     /locus_tag="JNZ27_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY8662509.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10886..11632)
                     /locus_tag="JNZ27_000020"
     CDS             complement(10886..11632)
                     /locus_tag="JNZ27_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662510.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11690..11878)
                     /locus_tag="JNZ27_000021"
     CDS             complement(11690..11878)
                     /locus_tag="JNZ27_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8662511.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12253..13482)
                     /locus_tag="JNZ27_000022"
     CDS             complement(12253..13482)
                     /locus_tag="JNZ27_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8662512.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRHIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13731..14852
                     /locus_tag="JNZ27_000023"
     CDS             13731..14852
                     /locus_tag="JNZ27_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY8662513.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRISDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14901..16127)
                     /gene="pepT"
                     /locus_tag="JNZ27_000024"
     CDS             complement(14901..16127)
                     /gene="pepT"
                     /locus_tag="JNZ27_000024"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY8662514.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16377..17513
                     /gene="potA"
                     /locus_tag="JNZ27_000025"
     CDS             16377..17513
                     /gene="potA"
                     /locus_tag="JNZ27_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY8662515.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKKLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17497..18354
                     /gene="potB"
                     /locus_tag="JNZ27_000026"
     CDS             17497..18354
                     /gene="potB"
                     /locus_tag="JNZ27_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY8662516.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18351..19145
                     /gene="potC"
                     /locus_tag="JNZ27_000027"
     CDS             18351..19145
                     /gene="potC"
                     /locus_tag="JNZ27_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY8662517.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19142..20188
                     /gene="potD"
                     /locus_tag="JNZ27_000028"
     CDS             19142..20188
                     /gene="potD"
                     /locus_tag="JNZ27_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY8662518.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20344..21165)
                     /gene="cobB"
                     /locus_tag="JNZ27_000029"
     CDS             complement(20344..21165)
                     /gene="cobB"
                     /locus_tag="JNZ27_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY8662519.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21181..22092)
                     /gene="nagK"
                     /locus_tag="JNZ27_000030"
     CDS             complement(21181..22092)
                     /gene="nagK"
                     /locus_tag="JNZ27_000030"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY8662520.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22121..23365)
                     /gene="lolE"
                     /locus_tag="JNZ27_000031"
     CDS             complement(22121..23365)
                     /gene="lolE"
                     /locus_tag="JNZ27_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY8662521.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23365..24066)
                     /gene="lolD"
                     /locus_tag="JNZ27_000032"
     CDS             complement(23365..24066)
                     /gene="lolD"
                     /locus_tag="JNZ27_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY8662522.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24059..25258)
                     /gene="lolC"
                     /locus_tag="JNZ27_000033"
     CDS             complement(24059..25258)
                     /gene="lolC"
                     /locus_tag="JNZ27_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY8662523.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25520..26566
                     /locus_tag="JNZ27_000034"
     CDS             25520..26566
                     /locus_tag="JNZ27_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY8662524.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26694..30140
                     /gene="mfd"
                     /locus_tag="JNZ27_000035"
     CDS             26694..30140
                     /gene="mfd"
                     /locus_tag="JNZ27_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY8662525.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30283..31221
                     /gene="ldtC"
                     /locus_tag="JNZ27_000036"
                     /pseudo
     CDS             30283..31221
                     /gene="ldtC"
                     /locus_tag="JNZ27_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31304..31561)
                     /gene="bhsA"
                     /locus_tag="JNZ27_000037"
     CDS             complement(31304..31561)
                     /gene="bhsA"
                     /locus_tag="JNZ27_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY8662526.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31802..32434
                     /gene="comR"
                     /locus_tag="JNZ27_000038"
     CDS             31802..32434
                     /gene="comR"
                     /locus_tag="JNZ27_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY8662527.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32496..33035)
                     /locus_tag="JNZ27_000039"
     CDS             complement(32496..33035)
                     /locus_tag="JNZ27_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8662528.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33262..34566)
                     /locus_tag="JNZ27_000040"
     CDS             complement(33262..34566)
                     /locus_tag="JNZ27_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8662529.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34967..35509)
                     /locus_tag="JNZ27_000041"
     CDS             complement(34967..35509)
                     /locus_tag="JNZ27_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8662530.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35532..36557)
                     /gene="nagZ"
                     /locus_tag="JNZ27_000042"
     CDS             complement(35532..36557)
                     /gene="nagZ"
                     /locus_tag="JNZ27_000042"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY8662531.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36568..37392)
                     /gene="thiK"
                     /locus_tag="JNZ27_000043"
     CDS             complement(36568..37392)
                     /gene="thiK"
                     /locus_tag="JNZ27_000043"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY8662532.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37373..38014)
                     /gene="lpoB"
                     /locus_tag="JNZ27_000044"
     CDS             complement(37373..38014)
                     /gene="lpoB"
                     /locus_tag="JNZ27_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY8662533.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38029..38406)
                     /locus_tag="JNZ27_000045"
     CDS             complement(38029..38406)
                     /locus_tag="JNZ27_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY8662534.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38409..38768)
                     /gene="hinT"
                     /locus_tag="JNZ27_000046"
     CDS             complement(38409..38768)
                     /gene="hinT"
                     /locus_tag="JNZ27_000046"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY8662535.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39102..41291
                     /gene="fhuE"
                     /locus_tag="JNZ27_000047"
                     /pseudo
     CDS             39102..41291
                     /gene="fhuE"
                     /locus_tag="JNZ27_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41351..42784)
                     /gene="ptsG"
                     /locus_tag="JNZ27_000048"
     CDS             complement(41351..42784)
                     /gene="ptsG"
                     /locus_tag="JNZ27_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY8662536.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43079..43876)
                     /locus_tag="JNZ27_000049"
     CDS             complement(43079..43876)
                     /locus_tag="JNZ27_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8662537.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43887..44891)
                     /gene="holB"
                     /locus_tag="JNZ27_000050"
     CDS             complement(43887..44891)
                     /gene="holB"
                     /locus_tag="JNZ27_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY8662538.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44888..45529)
                     /locus_tag="JNZ27_000051"
     CDS             complement(44888..45529)
                     /locus_tag="JNZ27_000051"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY8662539.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45519..46541)
                     /gene="yceG"
                     /locus_tag="JNZ27_000052"
     CDS             complement(45519..46541)
                     /gene="yceG"
                     /locus_tag="JNZ27_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY8662540.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46544..47353)
                     /gene="pabC"
                     /locus_tag="JNZ27_000053"
     CDS             complement(46544..47353)
                     /gene="pabC"
                     /locus_tag="JNZ27_000053"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY8662541.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47473..48714)
                     /gene="fabF"
                     /locus_tag="JNZ27_000054"
     CDS             complement(47473..48714)
                     /gene="fabF"
                     /locus_tag="JNZ27_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY8662542.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48801..49037)
                     /gene="acpP"
                     /locus_tag="JNZ27_000055"
     CDS             complement(48801..49037)
                     /gene="acpP"
                     /locus_tag="JNZ27_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8662543.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49248..49982)
                     /gene="fabG"
                     /locus_tag="JNZ27_000056"
     CDS             complement(49248..49982)
                     /gene="fabG"
                     /locus_tag="JNZ27_000056"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY8662544.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49995..50924)
                     /gene="fabD"
                     /locus_tag="JNZ27_000057"
     CDS             complement(49995..50924)
                     /gene="fabD"
                     /locus_tag="JNZ27_000057"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY8662545.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50940..51893)
                     /locus_tag="JNZ27_000058"
     CDS             complement(50940..51893)
                     /locus_tag="JNZ27_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY8662546.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51961..53031)
                     /gene="plsX"
                     /locus_tag="JNZ27_000059"
     CDS             complement(51961..53031)
                     /gene="plsX"
                     /locus_tag="JNZ27_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY8662547.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALMTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53112..53285)
                     /gene="rpmF"
                     /locus_tag="JNZ27_000060"
     CDS             complement(53112..53285)
                     /gene="rpmF"
                     /locus_tag="JNZ27_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY8662548.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53337..53858)
                     /gene="yceD"
                     /locus_tag="JNZ27_000061"
     CDS             complement(53337..53858)
                     /gene="yceD"
                     /locus_tag="JNZ27_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY8662549.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54057..54641
                     /gene="yceF"
                     /locus_tag="JNZ27_000062"
     CDS             54057..54641
                     /gene="yceF"
                     /locus_tag="JNZ27_000062"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY8662550.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54753..55712)
                     /gene="rluC"
                     /locus_tag="JNZ27_000063"
     CDS             complement(54753..55712)
                     /gene="rluC"
                     /locus_tag="JNZ27_000063"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY8662551.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56285..59470
                     /gene="rne"
                     /locus_tag="JNZ27_000064"
     CDS             56285..59470
                     /gene="rne"
                     /locus_tag="JNZ27_000064"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY8662552.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVHPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59667..60620)
                     /gene="flgL"
                     /locus_tag="JNZ27_000065"
     CDS             complement(59667..60620)
                     /gene="flgL"
                     /locus_tag="JNZ27_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY8662553.1"
                     /translation="MRFSTRMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60632..62274)
                     /gene="flgK"
                     /locus_tag="JNZ27_000066"
                     /pseudo
     CDS             complement(60632..62274)
                     /gene="flgK"
                     /locus_tag="JNZ27_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62340..63281)
                     /gene="flgJ"
                     /locus_tag="JNZ27_000067"
     CDS             complement(62340..63281)
                     /gene="flgJ"
                     /locus_tag="JNZ27_000067"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY8662554.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63281..64378)
                     /locus_tag="JNZ27_000068"
     CDS             complement(63281..64378)
                     /locus_tag="JNZ27_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY8662555.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64390..65088)
                     /gene="flgH"
                     /locus_tag="JNZ27_000069"
     CDS             complement(64390..65088)
                     /gene="flgH"
                     /locus_tag="JNZ27_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY8662556.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65141..65923)
                     /gene="flgG"
                     /locus_tag="JNZ27_000070"
     CDS             complement(65141..65923)
                     /gene="flgG"
                     /locus_tag="JNZ27_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY8662557.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66061..66812)
                     /locus_tag="JNZ27_000071"
                     /pseudo
     CDS             complement(66061..66812)
                     /locus_tag="JNZ27_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66832..68760)
                     /gene="flgD"
                     /locus_tag="JNZ27_000072"
                     /pseudo
     CDS             complement(66832..68760)
                     /gene="flgD"
                     /locus_tag="JNZ27_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68772..69175)
                     /gene="flgC"
                     /locus_tag="JNZ27_000073"
                     /pseudo
     CDS             complement(68772..69175)
                     /gene="flgC"
                     /locus_tag="JNZ27_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69179..69352)
                     /locus_tag="JNZ27_000074"
     CDS             complement(69179..69352)
                     /locus_tag="JNZ27_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY8662558.1"
                     /translation="MLDKLDAALRFQQEALHLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69507..70166
                     /gene="flgA"
                     /locus_tag="JNZ27_000075"
     CDS             69507..70166
                     /gene="flgA"
                     /locus_tag="JNZ27_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY8662559.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70242..70535
                     /gene="flgM"
                     /locus_tag="JNZ27_000076"
     CDS             70242..70535
                     /gene="flgM"
                     /locus_tag="JNZ27_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY8662560.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70540..70956
                     /gene="flgN"
                     /locus_tag="JNZ27_000077"
     CDS             70540..70956
                     /gene="flgN"
                     /locus_tag="JNZ27_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY8662561.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70996..72531)
                     /gene="murJ"
                     /locus_tag="JNZ27_000078"
     CDS             complement(70996..72531)
                     /gene="murJ"
                     /locus_tag="JNZ27_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY8662562.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72698..73564)
                     /locus_tag="JNZ27_000079"
     CDS             complement(72698..73564)
                     /locus_tag="JNZ27_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8662563.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLELRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73566..74213)
                     /locus_tag="JNZ27_000080"
     CDS             complement(73566..74213)
                     /locus_tag="JNZ27_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY8662564.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74224..74808)
                     /gene="rimJ"
                     /locus_tag="JNZ27_000081"
     CDS             complement(74224..74808)
                     /gene="rimJ"
                     /locus_tag="JNZ27_000081"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8662565.1"
                     /translation="MFGYRSNVPKVSLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75044..76252
                     /gene="mdtH"
                     /locus_tag="JNZ27_000082"
     CDS             75044..76252
                     /gene="mdtH"
                     /locus_tag="JNZ27_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY8662566.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76316..76963
                     /gene="grxB"
                     /locus_tag="JNZ27_000083"
     CDS             76316..76963
                     /gene="grxB"
                     /locus_tag="JNZ27_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY8662567.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            77097..77657
                     /locus_tag="JNZ27_000084"
     CDS             77097..77657
                     /locus_tag="JNZ27_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8662568.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77763..78809
                     /gene="pyrC"
                     /locus_tag="JNZ27_000085"
     CDS             77763..78809
                     /gene="pyrC"
                     /locus_tag="JNZ27_000085"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY8662569.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78883..79128
                     /gene="dinI"
                     /locus_tag="JNZ27_000086"
     CDS             78883..79128
                     /gene="dinI"
                     /locus_tag="JNZ27_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY8662570.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            79418..79672
                     /gene="bssS"
                     /locus_tag="JNZ27_000087"
     CDS             79418..79672
                     /gene="bssS"
                     /locus_tag="JNZ27_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY8662571.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79787..80905
                     /gene="solA"
                     /locus_tag="JNZ27_000088"
     CDS             79787..80905
                     /gene="solA"
                     /locus_tag="JNZ27_000088"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY8662572.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIHVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80953..81066
                     /locus_tag="JNZ27_000089"
     CDS             80953..81066
                     /locus_tag="JNZ27_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY8662573.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81327..81893
                     /locus_tag="JNZ27_000090"
     CDS             81327..81893
                     /locus_tag="JNZ27_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY8662574.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81897..82472
                     /locus_tag="JNZ27_000091"
     CDS             81897..82472
                     /locus_tag="JNZ27_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY8662575.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82514..83566)
                     /locus_tag="JNZ27_000092"
     CDS             complement(82514..83566)
                     /locus_tag="JNZ27_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY8662576.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83791..84711
                     /locus_tag="JNZ27_000093"
     CDS             83791..84711
                     /locus_tag="JNZ27_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY8662577.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLAWMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84883..85080
                     /locus_tag="JNZ27_000094"
     CDS             84883..85080
                     /locus_tag="JNZ27_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY8662578.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            85104..>86235
                     /locus_tag="JNZ27_000095"
     CDS             85104..>86235
                     /locus_tag="JNZ27_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8662579.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20701 a        22937 c        21222 g        21375 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttctcttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgtcgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgtatgtgct gtatgggtgc tcagtctctg atccctttca gaaattctat
     6601 caatgcatct gtctgttcag gatttactgc cagcatttca ccggatagcg cgcaacgaac
     6661 aaaaccatga tgatcctttt caatcagcgc ccccgtttcc agaaatgcgc ggtaatcagt
     6721 gatattcatc gtctccatgt tgtcagcatg gtatttatca cgctgtttaa gtatctcatc
     6781 aaatttcatc ggcattgcgt tttcctctgt tgtgtctgtt tgtttcagat agtaactatg
     6841 ccggaccgtg acgaaaagcc agtagtgctc cataccgttt caaatgacgc aaaaaaagcc
     6901 ggattgctcc ggctgcgtgg tgtggttctg atattcctac tctgctaatt tgctcagccc
     6961 catgtctgcg taagtgcgat ttactgcatt tctcaggtct gcgtagttct ccgttggcgg
     7021 ctccggtgtg cccttgctgc tctgttttac cagcgggtcg cccaccagcc gcccgtaagc
     7081 tgcaatttgt gctgtcatat tgccacctca gtgaagcgat ccggcattgt tctttttcat
     7141 ctcttccatc attttttcag tgagcattac gcactgaaac cccatgtttt tgatgtctac
     7201 gggtttcatg cctttgtcag tggatatcac aggcagaata tcctgataca catcgcggtc
     7261 agggtaacta agcacacaaa gcgcccatga tgccacgggt gaggatcctt cgtctgtttc
     7321 aacccaccag cctagtgtag ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt
     7381 gtaaaatttt tcaccaaatt ttggttcgct catgattcca cctctccggt tttaacgttg
     7441 attgttgtta cctgttccgc ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc
     7501 gccgtcgtgg tcatgaatgc gcttatcagt tcgggatgtg ctttcgcgta tccttccccc
     7561 gcgtggcggt ctatcgttct gattgccacc tttaaagcgt gctctgtcat gtctaacgcg
     7621 cgatatttcg gttctgttct gtctctgcgt tttttgagtg atttattaaa tttccctgaa
     7681 gtgtgcatat ttatttttac cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc
     7741 taccttagtg gccctcatgc cagtaatggc gctgctttca gcggggtaga gtgcttttat
     7801 ccactatcta ccccgtgtct acctccctgt ctgattcagg taaaatcagg tagagagggt
     7861 agatagtggg tagacagtaa aaaaaggcta cctacctaac ttaatgcact gaattaaatg
     7921 tatttttctt tactcaggta gacagggtag acagcaatta caaaaaatta taaaaacgcg
     7981 tcgcactcgt ctgttgttat tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata
     8041 tattcatgtc cgtaaacttt cgcggctggc ttcatagcct tgccaaagtc atttacgttt
     8101 agcggtttgc tcctgcctgc gtacgccata aacgccagat agacgcggta aaggctgttt
     8161 ctggtcgtgt acttcactga atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc
     8221 agaaaattca ggaactggca aaactcaata accggatccg tctgttgctt tattgccaga
     8281 gcttcatcac cgtcacgctg ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag
     8341 ttcgccagca atcggcggat aatgacaggg atttcagccg caatcttttc cggtagctcc
     8401 ctgtcttttt cggcctcact aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc
     8461 acacctccgg cccgttcggt gaatatcatc gggttattgt tggtcgccag caccaccgcc
     8521 ctgattacag ccgtgaaacg cttttcgtat ttcgggttaa tttccacggg gtcaccgccc
     8581 gtgattttct tgatgcccgt tccttcgcct gtatatttcg gctggtcagc caggacgata
     8641 agacgactcc cgacaacctg cggacgtcca ccagcatcat caagcgatgt catttcagcg
     8701 cttaccgtgt tctgtttccc tgccagaagg ctggctatgt gtgtgaatgt acttttaccg
     8761 ctcccgccgt ctccggtggc ctcaataaac atctgccagt cgtaccggtt cgccataatc
     8821 atgtacagcg cggcacatat acgcatcatc ttgcgctggt cttttccggc tgcgtgctca
     8881 agccatttat gaaagtttag cgcgttatcg cggatgttct cccctggtgc tggtggcgtg
     8941 tactcaatgc cgttgtgcgt ggtgatccag ttctccggcg tgtgcgggga aaattccccc
     9001 gttttcaggt caagcgcacc attggcgaac ggcagcaaat cgccggacgg ctcgcccatt
     9061 ggttcggcaa taacttttaa cgcttccacg gcgttattga ttacgcgctt gctgaaagtg
     9121 gccctgtgct ctgaatagat cgccaccatt tcgcggctaa gttccattgt gctgaccgga
     9181 caccataccc cgccgcgcca tacgtgaacg atttcacttt cctgatgcac acaaacgcca
     9241 tcaaagcgct cggcaagcag ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta
     9301 tttttctgct ccggcatggt aagccctgcg gcaatatttt cacgctcagc attcagatag
     9361 cgccgccagt tttcacactt ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga
     9421 atatctgccg ccgccagctt ctgaccaatc tttttggtct ccaccaaatc cagttctccg
     9481 gcctggtaca gccttacgcg tttttttcca tccggaacaa ttttcagtgc atcaagttcg
     9541 gcaagctgat ttggcccaag ccacacaggc ggcacattat cgccggatgc ggggccatcc
     9601 tgctcctgcc attgtttcgc gtgcgaccag gcatcactac ccgcaaagat aattacctct
     9661 gtgtctttgt gttttattcc gcgtggctgt ttttttacgt tcggtgccag tttcattttt
     9721 tacccctgaa tacgttaagc atctttttta tttcctgaat attggcgcgt gctttctccc
     9781 tgctggttgg tttactgcgg ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat
     9841 aaacaggcat cacgcaatca tattcgtaac cttcacggcg gtaggtgatg cgccgttctg
     9901 ccacgccttt aatcgttacc atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa
     9961 atttagggtg agtgttgcca ctggcagtta agccagaata tttaagtttc attattttta
    10021 ttctccggtg tgctgttctt tatatctgtc gtgcaataga tctatttctt gcagttccat
    10081 tattacaggc tcaagaagcg ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg
    10141 ttcattgtcg ccaagtgttt caagccatat gcgcaatatt tccagcatgt tttcactgtg
    10201 agaaagtgca agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca tattaatccc
    10261 cgtccgtcgt ttttcttaaa ataacctctg tcacgaaatc agcataatcg gcggcgatat
    10321 tcagtacatc aagcccgttg atttatattc tctcgtggaa agtaagaaaa aagccgctct
    10381 aataagttct ggcattgacg aaagcgcatc agccgcatca tcaggaacgc cggaaaattc
    10441 ctgtttcagg gaattaaaac gatcatcacg cataaccccc cccattttca caatcggcaa
    10501 caagaatatt tttagcgtca ttcagtgacc gcgttgcgat gtatcggata cattccaggg
    10561 cgaactgtgt ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg cattcaatat
    10621 caataagcgc gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt gtggttttat
    10681 tgtgcatagg tttccccctg acgaatacgg gcggcgaata cagcaacaca accggacggg
    10741 caacggctac gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg ggattctccg
    10801 gtactcagtg ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata
    10861 tgatttacgg caaccataac ggctcctagt ttacgttgtt ggttagaagc cccgttactg
    10921 ctcctacagt gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc attacacata
    10981 aatacattac atgaggtgta attaacgtgt caacacacaa aaacgaaagg cgtggtaatc
    11041 ctccgttcca gttccgttta gatcctgact tacgggaaaa gatggaaaag gcacaacaac
    11101 aggacggcga tgattctctt gctgcctgga taaagcgaat aatccgcaaa gaattacagc
    11161 aacgcggcat agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag gtactacggc
    11221 gcattgcttt acaggcgcac acaatgacca acgaagaatc aaccgaaaaa acatcacctg
    11281 cacgaaaaag acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt
    11341 ttgcccttga gaagtacctt aaagaatact catcaaagga gagaacttcg cagttatggc
    11401 aacgcactga accagactga ttgcattgcc caccagcctg atagcggcta tcatccccgt
    11461 gcttatgttt gggatcacac acacaaacgg cgcagcgggt tatctgttca gaaaggcggc
    11521 tccatttcgg ggccgctttt tttatgcctg aaaaacccca attttgtggt tttccagttt
    11581 caccagggcg aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc atggttatag
    11641 ctctgtgttc agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg
    11701 ccttgcagct atccttccag gccagaacct cggataaaga ccagccaacg gaacgaccgc
    11761 caagtttacg acgtgatggg aattgtccgg ccttttccag gcggtagcgg catgagcggc
    11821 taaggcctgt tagcttttcg cattcttttt cacgtataaa ccgatcagtg cttaacacta
    11881 ttgccccctt tcgtttctta aagagttatt tcgtgttcta ttgcgttggg atgtgtctga
    11941 ttgtgtcagg atgattcaga gttggcaaat gttgaggccg tatggtttac agaaagagga
    12001 acaatcagga taaaatcatt taaattcatg ttaatacaaa ggcataaaat attgtttcat
    12061 gccttttttc tcgctattta aagagtgatt cgctagtgta taaaaaacca gtaacacgtt
    12121 aaaaatcagc tacttataaa tctgtgcact ttttcacctc ttgttcgtag ttgttccgca
    12181 ttgttgctca ttgttgcacg ttgtatctgt tcgcatatcc agtatgcgca tactgaaaaa
    12241 acacgaaaaa aattattttc ttctggctac tggtagcgtg gttacgtttt catgtgttcc
    12301 cgccagtatc cctaaccgct ccgtccacat atccagcgca ttgcgtttag catccagata
    12361 acgggaatga ttataaactc gctgcattcc tggcatctgg tggcctgtaa gctgctccac
    12421 gacgtgagga tccacgccta aatcgttcag catcgttgta aaggtgcgcc ggatgtcatg
    12481 tagtgaccag tgagggtgat ttagcctcct gtgcgctaat ctgccgtact gcgacacgct
    12541 tgtttcctgt ttcacttccc ccagcaataa gcccgtgtgc ctgttctgct ccaccagctg
    12601 cgtgacgaac ggcagtattg cttccggtat gtgccggaat attgccacct tcgttttgct
    12661 gtgttctttc ggcacggtcc agagcatttc ggtaaaatcc cactcgctga tctccgataa
    12721 cctcagttct accgtccggc atccgaacac aatcaggagg cggattaacg cgatgtagta
    12781 gggggagaat atttttttgt ccagtgcctg caataattcg cccagttctt tggtgcttaa
    12841 gacacgctcg cttatatccg gttttttccc aacgtccgcc acactcatat catcaagaac
    12901 gttgctgatt gcatagcgcc gcctccggca gaacttaagc gcctgtttgc acgtctgtag
    12961 caagaatccg gcagtaacag gcgttcgctt tgccacctgg tcaaaacagg ccagccagtg
    13021 ccgtagctcg catttatcca gcggcatagc accaatgtgc tgtattacgt gattattaag
    13081 gcgctttttc agggcggcat aatccacgcg gttttccttt gcgtacgact caagccagta
    13141 ggtgagcgca tcgccaaccg ttaccggctt taacgcttcc tgtacggtgt aattaagctc
    13201 atgacgtgga tttttcccct ctgccagcca tgcgcgacac tgggcggctt tttccctggc
    13261 tgatttcagg ctcagatcag gataatttcc cagcttaatg cgttccggtg gtgcccccct
    13321 tcccgttccg gccctgtaag tgaaatacca ggttaaaagg ccactggttg aatgcctgac
    13381 gctcaggttt ccaccgtcat taagaaaggc tgttttttgg gcgggtgtgc cgttgatttt
    13441 cctcagctgt gtatcgctca gtttgttaag tgctctgctc ataattttga ttccggtcat
    13501 cacaattaac tacaccatta actacaccga tcgttgcaca aagggctaca acgtgaaaca
    13561 agctggaaca aggaaaatca caaatctgtt tataatcaaa aacataatga acaatctgaa
    13621 acattatgaa acggcaaaaa acactaattg ataaaatatg tccatacttc acgcattacg
    13681 ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac atggaatacc
    13741 aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc ccggtggtgt
    13801 taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg gcaggtctgg
    13861 cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa tggcaggtca
    13921 gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca ttactggtac
    13981 aggcagtaaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac
    14041 cggactggcg tattgatgat ctgatgattt ctttttctgt acctggcggc ggcgtcggcc
    14101 cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggacgtcgt cgctggcgag
    14161 tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc agatctgtta caggtcgatc
    14221 cgttcgaagc catcatcgat gaagagctgg agcctggtga tattctttat attccgccag
    14281 gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg
    14341 cgccaaatac gcgggaatta attagcggat ttgccgatta tgtgctgcaa cgtgaactgg
    14401 gcggcaacta ctacagcgat ccggatgttc cacctcgcgc tcatcctgcg gatgttctgc
    14461 cgcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag ccggaacact
    14521 ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat atcgcgccgc
    14581 cggaaccgcc ttatcagcca gatgaaatct acgatgcact gaaacaaggt gatgtgctgg
    14641 tgcgcctggg tggtctgcgc gtattgcgca ttagcgacga cgtgtatgcc aatggtgaga
    14701 agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt gcgctgactg
    14761 cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg
    14821 tcaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga tgcagcatca
    14881 catgccgcat cccttttcgc ttacttccgt tgcgccgtta actcggcaat acggacgatc
    14941 acctgcaccg ctttttccat accttccaga atcacaaact catgcttacc atgatagttg
    15001 taaccgccag tgaacaggtt cgggcaaggt aatcccataa acgacaactg cgcgccgttg
    15061 gtaccgccgc ggatcggttt cagttccggt tcaatatcgc agtcacgcat cgcctgctgg
    15121 gcgatatcga gaatatgcgg atgctcaacc actttctcgc gcatattgta gtaactgtct
    15181 tcaatcacca attcaatgta gcaatcagga tgtaaccctt tgcccacttt tttggcgatc
    15241 tccatcattt tacgtttacg cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag
    15301 tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca ggtgatagaa accttcatag
    15361 ccttctgtca tttccgggct ttcatccgcc ggaacttccg catgaatacg tgccgccagc
    15421 gacagcgcat ttaccatcac tcctttcgcc gtgcccggat gaacattgtt accgacaatt
    15481 ttgatattga ccgatgcggc gttgaagttt tcaaactcca gttcgcctac gccaccaccg
    15541 tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat caaaatgttt cgcccctttg
    15601 cccacttctt catccggggt aaaggcgacg cgaatatcac catgcggaat gtttttctgt
    15661 tgcaataccg ccagcgcggt catgatttct gcaatacctg ctttgtcatc ggcacctaac
    15721 aaggttttac catcggtggt aatcaacgtc tgacccagta gctgatgcag caccgggaac
    15781 ataaccggtg ataaaacttc atcgccgata cccagcgcaa tatcgccacc gcgatagttt
    15841 tcaacaattt gcggattaac atttttgccg ctgcaatccg gtgaggtatc cacatgagaa
    15901 ataaagccaa tcgccgggat atcgccaggg acgttagccg gtaacgtcgc catcaaagtg
    15961 cccttctcac ttaaggtcac attgataagc cccatttctt cgagctgctc tttcagcaga
    16021 tgcaataact tccattggcc ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg
    16081 gtatccagag acacgtagtt caaaaatcgc tcaagtagtt tatccatgta gtcaccctca
    16141 ctttttgtga caacattatt aagaagcaag aaaagacaaa tattgcgtca ggtcactttt
    16201 acccctgcaa gcgggaatat ttatcagcat taccttaatg aatataaagc taagccagta
    16261 attcacaaca aagattggcg attcaagcgg tttgccgtag aattaccgcc ctcattaaga
    16321 gtcaccaagg tggttaacca caaaccccgc atcggtaagc catccgttgc gtttacatgg
    16381 gacagagtaa aaaattgaat aaacaaccga gttcgctttc accgctggtg caattggcgg
    16441 gaattcgcaa atgctttgat ggtaaagagg tcattcccca gctggatctg actatcaaca
    16501 atggcgagtt cctcacgctg cttggccctt ctggctgcgg taaaacaacc gttcttcgcc
    16561 tgattgcagg tctggaaact gttgattccg gacgcatcat gctggataac gaggacatca
    16621 cccacgttcc ggcggaaaac cgctatgtga acactgtttt ccaaagctat gcacttttcc
    16681 cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg catgcaaaaa acccccgctg
    16741 ctgaaattac gccccgcgtg atggaagccc tgcggatggt gcagctggaa accttcgctc
    16801 aacgtaaacc gcatcagctc tctggtggtc aacagcaacg cgtcgccatt gctcgcgcgg
    16861 tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct ctcagcgctg gattacaaac
    16921 tgcgtaagca aatgcagaac gagctgaaag cgttacagcg taagcttggc attacattcg
    16981 ttttcgtgac tcacgaccag gaagaagcac tcaccatgtc ggacaggatt gtggtgatgc
    17041 gcgatggtcg cattgagcaa gacggcacgc cgcgtgaaat ctacgaagag ccgaaaaaac
    17101 tgtttgttgc cgggttcatt ggcgaaatta atatgtttaa cgccacggtg atcgagcgac
    17161 tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga atgtaatatc tacgttaact
    17221 tcgccgttga accggggcaa aaactgcatg ttctgctgcg cccggaagac ttacgtgttg
    17281 aagagattaa cgacgacaac cacgctgaag ggctgattgg ttacgttcgt gagcgtaact
    17341 acaaaggcat gacgctggag tcggttgttg aactggaaaa tggcaagatg gtgatggtca
    17401 gcgaattctt caatgaagac gatcctgact ttgaccactc tctcgaccaa aaaatggcca
    17461 ttaattgggt agaaagctgg gaggtcgtac tggctgatga agaacacaag taagttccag
    17521 aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg
    17581 atgatcattg gcaccagctt tttgacccgc gacgacgcca gtttcgtcaa aatggtcttt
    17641 acgctggata actacacgcg tctgctcgat ccgctctatt ttgaagtgct attgcactcg
    17701 ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc tgggctatcc atttgcctgg
    17761 tttctggcga agctgccaca caaggtgcgt ccgctgctgc tgtttctgct gattgttccg
    17821 ttctggacca actcattgat tcgtatctac gggctgaaaa ttttcctcag caccaaaggc
    17881 tatctcaacg agtttttgct ctggctgggc gttatcgaca caccaatccg tatcatgttc
    17941 acgcccagtg cggtgattat cggtctggtt tacattctgc tgccgtttat ggtgatgccg
    18001 ctgtactcca gtatcgaaaa actggataag ccgttgcttg aggcggcgcg cgatctcggt
    18061 gccagcaagt tacagacttt tatccgcatc attattccac tgacgatgcc ggggattatt
    18121 gccggatgtc tgctggtgat gctgccagcg atgggcctgt tctatgtgtc cgacctgatg
    18181 ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg tacagttcct caatattcgt
    18241 gactggccgt ttggtgcagc caccagcatt acgctgacta tcgtaatggg cctgatgttg
    18301 ctggtttact ggcgcgcttc tcgtttgctg aataagaagg tggaactcga atgatcggtc
    18361 gactgcttcg cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta
    18421 ttttgattgt gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca
    18481 ccaaatggta tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac
    18541 taacaatggc ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg
    18601 cgctgtaccg ttatcgcttt cgcggtaagc cgttcgttag cggaatgctg tttgtggtaa
    18661 tgatgtcacc agatatcgtg atggcaattt cgctgctggt gctgtttatg ctgctgggta
    18721 ttcagcttgg cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg
    18781 tggtgacggt gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag
    18841 atctcggtgc cagcgaattt accattctgc gaaaaatcat tctgccactg gcaatgccag
    18901 cggtggcggc gggctgggtg ttaagcttta ccctgtcgat ggacgatgtg gtagtttctt
    18961 cattcgtcac cggaccaagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag
    19021 tcggcgtatc gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga
    19081 tggttattgc cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta
    19141 aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc
    19201 cgctcacgcc gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc
    19261 aggactgctt gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga
    19321 gtcgaacgaa accatgtacg ccaagctgaa aacgtacaaa gacggtgcct atgacctggt
    19381 ggttccttca acctattacg tcgataaaat gcgtaaagag gggatgatcc agaagatcga
    19441 caagtcgaag ttaacgaact tcagcaatct cgatccagac atgctcaaca agccttttga
    19501 cccgaataac gactactcca ttccgtatat ctggggtgcg acggcgattg gtgttaacgg
    19561 tgatgcagtg gatccaaaat ctgtcaccag ctgggccgat ctgtggaagc cagagtacaa
    19621 aggcagcctg ctgttgaccg acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct
    19681 gggctactcc ggtaacacca ccgatccgaa agagattgaa gctgcatata acgagctgaa
    19741 aaaactgatg ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc cgtacatgga
    19801 aggcgaagtt aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg
    19861 tacgcctatt gacgtggtgt ggccgaaaga aggcggcata ttctggatgg acagcctggc
    19921 gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg
    19981 cccggatgtg gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc
    20041 gcgtaagctg ttaagtccag aagtggcaaa cgataagacg ctctacccgg atgctgaaac
    20101 cattaaaaat ggcgaatggc agaatgacgt tggcgcagcc agcagcattt atgaagagta
    20161 ttatcagaag ctgaaagcag gacgttaatt cagcaagcgg gcggtggtat taccgcccgt
    20221 tttttagtta gcaaggatgt ggctaatgag tcaggacagt aaagttttat cccgctcttt
    20281 cgtcggtatt gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct
    20341 gctttataat ccctccagca acttttcaac aaattctggc accacctggc ttgccgggcc
    20401 gtaatatttc tcggcaaatt cattaccaac ctgactcggt tcaagattca gttcaacggt
    20461 gtgagcacca tgtagtttgg cttcgtgtac aaacccagca gccggataga catgcccgga
    20521 agtaccaatg gcaataaaga catccgccat cgacaacgcc atataaattt catccatgcc
    20581 gagtggcatt tcgccaaacc aaacgacgtg cggacgcaag ggtgccggga actggcaaca
    20641 atggcattta tcttctggcg taacgtctcc ggtccagtcg agaacctgac cactttgcga
    20701 acaacgaact ttcagcaatt ccccatgcat atgaatgaca ttggtattac ctgcgcgttc
    20761 atgcaggttg tctatattct gcgttaccag caaaaagcga tcgccgagag cgtcttgcag
    20821 tttagccagc gcaagatgcg cggcgttagg ctgaatttct ggctgctgca gctgtcgacg
    20881 acgggcgtta taaaacgctt gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc
    20941 cacatcttca acccgatgtt cttcccacag gccatctgcg gcgcgaaagg tacgaatacc
    21001 tgattccgca gaaattcctg cccctgtcag tacgagtact cttggttttt ccattgcttc
    21061 cggcaccact ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcgacgttt
    21121 atttttacga aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttgt
    21181 ttaatcggtt agatgtagga aggccgcacc acgcattcct cccgcatcac cgtggcgcgc
    21241 gcgttcaatg cgcggaacac gagctacagg taagagatga caaggcaacc tgtccgccag
    21301 ttgcgttgtg attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc
    21361 aacaatggtc aggatatttc ccagagaaac cgctaataaa tccagataac gctcaacgtg
    21421 cgcccttgcc tgctcatcgc cttgatcata aagcgcaata atttcgggag cctgcaacgg
    21481 ttgatgataa tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat
    21541 gcagccatgc tgaccacagc cgcagcggcg taacgggaaa tccaacccca tcatggttaa
    21601 cgcatcaacc ggcagacgca tatggccaaa ctcgccggta atgtagcttt taccagtaat
    21661 cggtttgccg ttgaaaacca gcccgccgcc aacgccggtg ccgagaatta accccatcac
    21721 cagtggatat tgcgtaaatt catcctccca ggcttctgaa agggcaaaac agttggcatc
    21781 gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttgcc
    21841 gctggcagcg gggacattag cggcatacag cttaccatct tctgtttccg gcattcccgg
    21901 aataccgatg ccgacagaac ctttacagcc aaaacgttgg tcagcttcag ctaccagctc
    21961 acacactgca tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca
    22021 ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt tttgttccac caatatcaaa
    22081 cccgtaatac attgccgctc cttgttttaa tgtactacct ttactggccg ctaaggactc
    22141 gcgcagggtc aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca
    22201 acaatgctgt gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaggaagt
    22261 caataaaata gatatcgctg gagaggaact gatgaccgat cagcttttca atccactcaa
    22321 taatcggggt aagttgcagt gaaacaacta cgccgataac caccccacac aggctgccga
    22381 acagccctgc cagcaatcca taccagacaa agatggcgcg aattaaacca tctttcgccc
    22441 ccagcgttct taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg
    22501 agacgatgtt gaaacaggcc acgccaatca ccagtaccat cgccagatac ataatggcgc
    22561 ggatcatctg gatatcgcga tacatatagc cgtaagtacc aatccaactt ttaatataaa
    22621 catagctgtt ggtcacttca cccgcatcgc gtaccagctt attggcgttg aaaacatccg
    22681 tcattttaag ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg
    22741 ccagcgggat catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa
    22801 tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc gggattcgag ttggggatca
    22861 tgatcgacac ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct ttgccgatga
    22921 taatttgctg ttcgcccgct ttaaaattgc gccacgcatc cccctgaaca aacgagggta
    22981 atgcgctcag acgctgttcc tgttgtgggt taacgccctt cacctggatt gcgcgcagat
    23041 tcgctccact ttccaccaga ccggtgaaat tgatatacgg cgcagcggcg gcaatacccg
    23101 gcactttctg cacgttatcc agtgcttcct gccagttagt ccatggctga tttaccgctt
    23161 cgatttcgcc atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt
    23221 tcatcgcgct taagccgacg atcaataccg ccacgccgag ggcaatgcca atggtagaaa
    23281 tgacagagat tagcgacacc atgccgccgc gtcgccgtcc gcgactaaaa cgcaggccaa
    23341 tcaataacga taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag
    23401 acgtccatca cgcatttcca gttggcggct catacgtttc gccagttgca ggtcgtgagt
    23461 gaccaccagg aaagcggtgc cctgcaagcg attcaactct ccaagcaact ggaagatgct
    23521 gtcggcgtta cgcgcatcga ggttaccggt aggttcatcc gccagtacca gacgcggatt
    23581 attgaccagc gcacgggcaa tcgctacacg ctggcgttcg ccgccagaaa gttcagatgg
    23641 gcggtgattc gcacgatgct ccagccccac cgcttttaac atctccagtg cacggctgtt
    23701 gatttcagcg ggctttttct tgccaatcag cagtggcata gccacgtttt ccagggcagt
    23761 aaaatccggc agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc
    23821 tgctttcgcc gctgaagaca gtttgctcat cggctgacca ttaaagatca catcgccgga
    23881 ggttggcgta tccagcccgc ctaacaggtg cagcaaggta cttttaccgg aaccagagct
    23941 accgacaatt gccatcattt caccttcgcc cacgctgaaa ctgacattgt gcagcacatc
    24001 ggtttgcaca ctgccttcct gatagcgttt gcacaggttg tcgcattgca acaggatctt
    24061 attcataacg taaagcctca gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg
    24121 tagacagcag cgcgatagcc atcgccacca gcgcaataac aatgacctgt aaaggttcga
    24181 tagccaccgg cagcgccgcg ccatcaagca ggacgccgat tatcggcatc agattattta
    24241 actggctggc aagcagtgcg ccaagcgccg ctccgaggat tgcaccgata atcccggcgc
    24301 tggccccttg caccataaag accatcatga tttgtcgcgg agttaagcct tgcgtttgca
    24361 ggatcgcgac ttcgccctgc ttctccatca ccatcagccc cagcgaggta ataatgttaa
    24421 acgccgcaac agcgacaatc aggctcagca gtaagcccat catatttttt tccatgcgta
    24481 cggcctggaa cagctcgcct ttacggtcac gccagtcctg ccatttgctg ccttcaggca
    24541 gtttttgctg acttaaagag tcaactttca gcggctcatc cagccacaaa cgccagccgg
    24601 taatattggc tgccggataa cgcatcagac gcgaggcatc ctcaatattc accagcattt
    24661 catagccatc gacttcactg ttggcggcga aagtgccaat cacattgaac aggcgctgac
    24721 ttggaatacg ccccatcggc gtgaactggc tggcagatgg caccatcacg cggatttgat
    24781 caccgcgatt gacgcccaac tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc
    24841 ccggctcgag gtcagtttgt ttcacattga ccagatacgg cgttagtgga tctttttgcg
    24901 ccgggtcgat accgagcatc accccgaccg ccacgctgcg cgcgctttgc agtaccacat
    24961 caccggtagt aataggtgcg acgcgattaa cgccgtccag tttgactgcc gtttccggga
    25021 gttgctgcgg gttaagagag ccatgctcag aagagagaat tgcctgtggc atcaggccaa
    25081 ggatgttgtt ttgcagctcg cgctcaaagc cgttcatcac tgacaatact gtgaccagcg
    25141 ccatcacccc gagggtaatg ccgatggtag aaagccagga gacgaaacga ccgaagcgat
    25201 ccgctgcacg cccacgcatg taacgcaagc caataaatag agcgacaggt tggtacatga
    25261 aatccgtctg gttgctgtag caaagacacg agtatataag cgaaagcaag atgggtaaac
    25321 gtctgaactg taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat caatcggcta
    25381 aattgccatg ttttatcatc aatcttgccg cctgatctta atctgaaaaa tcatccaata
    25441 cagacagata cccattacac ctcgggtcat aaccagcagg atcaaccgca tgtaaattaa
    25501 aaaagagcgt gttgttctga tgaaacaaaa agagctatgg attaaccaga tcaaagggtt
    25561 atgtatttgt ctggtggtga tttatcactc ggtcattacc ttttatccgc atatgaccac
    25621 ttttcagcat ccgttatcgg aagtcctgag caaatgctgg atctatttca atctttacct
    25681 tgcccccttt cgtatgccgg tttttttctt tatatccggc tatttgattc gccgctatat
    25741 cgacagcgta ccgtggggaa attgtctcga taaacggatc tggaacatct tctgggtgct
    25801 ggcgctttgg ggcgtggtgc agtggctggc acctgagcgc gatttaagca atgcctccaa
    25861 tgccgcttat gccgattcca ccggtgagtt cctgcacggg atgatcaccg ccagcaccag
    25921 cttgtggtat ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt ttagccgtct
    25981 ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg gcggttaatt tcgttcccac
    26041 gccgtggtgg ggaatgaaca gtgtgatacg caatttgcct tattacagcc ttggcgcatg
    26101 gtttggcgca acaataatga cctgtgttaa agaggtgccg ttgcgccgcc atctgctgat
    26161 ggcttctttg cttaccgttc tggcggtcgg tgcctggttg tttactatct cgctgctgtt
    26221 gtcgctggta tcgattgtgg tgatcatgaa gctgttttat cagtacgagc aacgattcgg
    26281 tatgcgctcc accagcctgc tgaatgtgat tggctccaac accattgcta tctacaccac
    26341 ccatcgcatt ctggttgaaa tattcagctt aactctgctt gcgcaaatga acgcagcacg
    26401 ctggtcgccg caagtcgaac tgacactcct gctggtttat ccctttgtca gtttgttcat
    26461 ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgtgcattca gcgatctgtt
    26521 gttctccccg ccttcactgc ccgctgccgt cagttactct cgttaaacca tcaccccagc
    26581 cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca ttgcttatca
    26641 gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc gttatgcctg
    26701 aacaatatcg ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg ggcgagttaa
    26761 ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc ggtccggtgg
    26821 tactcattgc accagatatg caaaatgctc tgcgtttgca tgatgaaatc agccagttca
    26881 ccgatcagat ggtgatgaat ctggcggact gggaaactct tccctacgac agtttttcgc
    26941 ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg acgatgcagc
    27001 gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgccca cacagttttc
    27061 tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat gcattacgaa
    27121 cccaactgga cagcgccggt tatcgctatg ttgaccaggt gatggagcac ggcgaatacg
    27181 ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct tatcgtcttg
    27241 atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc cagcgcacgc
    27301 tggaggaagt agaagcgatc aatctgctgc ccgcacacga atttccgacc gataaagcgg
    27361 caattgaact gttccgcagc cagtggcgcg ataccttcga agtgaagcgc gatccggaac
    27421 atatttacca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac tggcagccat
    27481 tgttcttcag cgaaccactg ccgccgctgt tcagttattt ccctgccaat accttgctcg
    27541 tgaatactgg cgatctggaa accagtgccg aacgtttcca ggctgacacg ctggcgcgtt
    27601 ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg ctctggctgc
    27661 gggtggacga gctcttctca gagctgaaaa actggccccg agtgcagcta aaaactgaac
    27721 atttaccgac aaaagccgcg aatgccaatt taggtttcca gaaactgcca gacctggccg
    27781 ttcaggcgca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag actttcgacg
    27841 gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcgctgggt gaactgctcg
    27901 cacgaattaa aattgctccg caacgcatta tgcgtcttga tgaagccagc gaccgtgggc
    27961 gttatctgat gattggcgct gccgaacatg gttttgtcga taaggtgcgt aatctggcgc
    28021 tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag gattctcgcc
    28081 gcaccatcaa ccccgataca ctgatccgta accttgcgga gctgcatatt ggtcagccgg
    28141 tggtccatct ggagcacggc gtcggtcgct atgccggaat gaccacgctg gaagctggcg
    28201 gcattactgg cgagtacctg atgctcacct atgccaacga cgccaaactg tatgttccgg
    28261 tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac gccccgctgc
    28321 ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa aaagtgcgtg
    28381 atgtggcggc ggaattgctg gatatctacg cgcaacgcgc cgccaaagag ggcttcgcgt
    28441 ttaaacacga tcgtgagcag tatcagttgt tctgcgacag cttcccgttt gaaaccacgc
    28501 cggatcaggc gcaggccatt aatgcggtac ttaacgacat gtgtcagccg ctggcaatgg
    28561 atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg cgcgccgctt
    28621 tcctggcagt agataaccac aagcaggtag cggtgttggt gcctaccacc cttcttgcgc
    28681 agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt atcgaaatga
    28741 tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg gcggaaggga
    28801 aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag tttaaagatt
    28861 taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa gagcgcatta
    28921 aagcgatgcg cgcgaacgtg gatattctga cgcttactgc aacgccgatc ccacgtacgc
    28981 tgaatatggc aatgagcgga atgcgtgacc tgtcgattat cgccacgccg cccgcccgtc
    29041 gtctggcagt taaaaccttt gtccgtgagt atgacagcct ggtggtccgg gaggcgatcc
    29101 tgcgtgaaat tttgcgcgga ggacaggttt attatctcta caatgatgtg gaaaacattc
    29161 agaaagccgc cgaacggctg gcagaactgg tgcctgaagc acggattgcc atcggtcacg
    29221 ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat caacgtttca
    29281 acgtgctggt ttgtaccacc attatcgaaa ccgggatcga catcccgaca gccaacacca
    29341 ttatcattga acgtgcggat cacttcggtc tggcgcagct gcaccagtta cgcggtcgcg
    29401 tcggacgttc acatcatcag gcatatgcat ggctgctgac gccgcatcca aaagcgatga
    29461 ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc ggggcaggtt
    29521 ttgcgctggc aacgcacgat ctggagattc gcggcgcggg tgaactgctt ggcgaagaac
    29581 aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg gaaaacgccg
    29641 tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc cagcaaacag
    29701 aagtcgagct gcggatgccg tcgctattgc cagatgattt catccctgac gtgaacacgc
    29761 gtctgtcgtt ctacaaacgt attgccagcg ccaaaacgga aaacgaactg gaagagatca
    29821 aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc ggcgcgtacc ctgctggata
    29881 ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa ggtaatgaga
    29941 aaggcgggat gatcgaattt gccgagaaga atcacgttaa tccggcctgg ttgattggtt
    30001 tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg aaatttattc
    30061 aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg cgtgaactgg
    30121 aagagaacgc gatcgcttaa tgtaaatcgg gggcgtttct gcgcccccat ctgcaacatt
    30181 tacaaattct ttgcactttc ctgcactatc cgacactgtc accatccata attcaggctt
    30241 atctgtttat tacaataacc ttatatttat tatggatttt tggtgatgat caaaacgcgt
    30301 ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctggc gctaccggca
    30361 aaagccaaca cctggccgct gccgccagcg agcagtcgtc tggttggcga aaacaaattt
    30421 catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata taacgtcggc
    30481 tttctcgctc tgttacaggc taaccccggc gttgatcctt acgtaccgcg cgcgggcagc
    30541 gtgttaacga tcccgttgcc aaccctactt ccagacgcgc cgcgcgaagg cattgtgatc
    30601 aacattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt aaccgtgtat
    30661 ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt gaccaccgtt
    30721 tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acatccgcgc ccgttataaa
    30781 gcacagggaa ttgagttgcc tgcggtagtg ccggctggac cggataaccc aatgggccat
    30841 catgcgattc gtctggcggc ctatggcggc gtttatttgc ttcacggtac gaacgccgat
    30901 ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga cgatatcaaa
    30961 acactcttta gctaggtcac cccaggcacc aaagtgaata tcatcaacac tccgataaaa
    31021 gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct gtcagagaag
    31081 attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca atcatttaaa
    31141 gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg ccgtcatcaa
    31201 gtgagcccac aaactctgta aatcctggat aaaaaaaatc cccgcgacac ggcatgtcgc
    31261 ggggaaaaaa tcagcagtag caggcattaa tgagggttaa tgcttattta taaattactg
    31321 ctgttccatg gagggtattc ggaccggtta cagaagtaat acggaaagat tttgcgccca
    31381 tctcatccgc tttttgcgcc agctgctctt ccagcgatcc cagatttgtc cccgcgttag
    31441 cactgatagt accgactttt tgttggcctt ctggcgttga ctgaacttcg acagccgcaa
    31501 agttggcaaa tgacatggag cttaaaatcg ccgcagcgat gagggttttt acgtttttca
    31561 taatagtggc cttatgcaga tgaatgacgt cgcaagaaat tacttaacga tcgataacta
    31621 aatgatagat gtgatctgga tcacatacaa gatatttttt ataacaatca ttaattaatt
    31681 taaaaatctt taaatttcag tcaaatagaa agcaattatt tttatgggat ctggactggt
    31741 gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt acaacggcat
    31801 catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa aagtgttcga
    31861 cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt atgaagcgac
    31921 ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat acgcggaatt
    31981 taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc gttttgccgc
    32041 taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc tggctgacta
    32101 ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct gcttcatgat
    32161 caacaactgc accaccctct ccccagattc aggagatatc gccaatacgt tgaaatcacg
    32221 ccatgcgatg caagaacgca ctttgcagca gtttttatgt caacgacaag cgcgcgggga
    32281 aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta ttattcaggg
    32341 gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctgatgcaga ttgccggaac
    32401 gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc ctcagcctga
    32461 gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt caaaatcagt
    32521 tgcccgttac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg gattttgccc
    32581 tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga cttgtcatac
    32641 accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag agagccctgg
    32701 atctggttac cggcatatcc accacccagc gcccccacaa cagtggcgac atctttaccg
    32761 cgaccaccac caaactgatg ccctatcacg ccaccagcaa cagcgccgag caccgacccg
    32821 gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt gcgacactcc
    32881 tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg agcgtattgc
    32941 gggccccgtt caaacacgtt cagactggcc actgccgcta cgcccaacgc agctgcgaca
    33001 ccaatcccga tacccgccaa cattgattta ttcacgggaa cacctccttc tttgcagtta
    33061 tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa aagcgcggga
    33121 atgactggaa atacttaagg attcggagga gtctgaagga atgcctgatg cgacgcttaa
    33181 cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggataagg cgtttatgcc
    33241 acatcagcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa cgcggttaat
    33301 actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa tctgatgcat
    33361 tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca ttgagccgcg
    33421 cgtcaggtta cccatcaggc taccaacggt ggagaagttc gacagcgata ccagcgaacc
    33481 atgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca gaatgttgtt
    33541 cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa agcccccttc
    33601 cggacgcggg catgacgcgc agtcgccaat agcgtaaatg tctggatcgc gagtggtttg
    33661 cagcgtcggt tccaccacca gctggttgat acggttagtt tcaagaccac cgatatcttt
    33721 caggaagtct ggcgctttga tcccggctgc ccataccatc agatcagcct caatatattc
    33781 gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt gcgtcagcac
    33841 gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca gagatacgtg gcggtaatgc
    33901 aggcaaaata cgttctcccg cttctaccag cgttacgttc agggcttcgt tggtcaggcc
    33961 tttgtaaccg tagctgtgca gttgcttgat cgcgttgtgc aattcagcgg agagttctac
    34021 acccgtcgcg ccgccgccga caatcgcaat gttcactttg ccatttgcgc ccaggttggc
    34081 agagtatttc aggaacagat tcagcatctc ctggtggaag cgacgcgcct ggtgcgggtt
    34141 atcgaggaaa atgcagttct ctttgacgcc tggcgtattg aaatcgttag aggtgctacc
    34201 cagcgccatg accagggtgt cataggcgat tttacgttcc ggaaccaaca gttcaccttt
    34261 ctcgtcgcgt agttctgcaa tagtgattgt tttcgcttca cgatcaatat caatgacgga
    34321 acccaactgg aactggaaac catgattgcg cgcatgggcc agatagctca acgcatcgac
    34381 gccttcatca agcgagccag tcgccacttc gtgcagcagt ggtttccaca ggtggctgtg
    34441 gttacgatcg accagcgtaa ttttggcttt tttcttacgt cccagcttat gccccagctg
    34501 tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatctttt tcaatggcgt
    34561 agtcaacgtg acccccttaa atttattaac caattgttaa ttaatggtta ttaacatagc
    34621 tcttaattaa caacaggtta cagcaatgaa aatgttcttc gctattgaga ataacatgaa
    34681 tggtgcattg gtcataccaa aattgatgtt aatcaagttt tgttgcttaa aagataggca
    34741 actttaccgg gggagatcaa aaaatagcgt ttattatttg agcaaattcg gaagagtaat
    34801 atagggtgaa gcgtatatcg agggcgtttg gtcgtcctga taagatgcgc agcatcgcat
    34861 caggcattga tgctcgactg tcggatgcgg cgtggacgcc ttatccgact tacagaattc
    34921 ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt
    34981 tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc gtctgctctt
    35041 cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagtg
    35101 cttcatcatt acgcgacaaa ataaccagac aacgatcgcg attcttctcg cggaagttgg
    35161 tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt
    35221 tctcataagg gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa
    35281 tccgttccgc ccagtatccg cccaggccaa cgccgcaaat tagcggacgc tcgtcaacgt
    35341 tcagttgcaa cattttgtcc acttctttaa gcagatgctg catatcatgt ttcggatgcc
    35401 gcgtactgta gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct
    35461 cgtggttacc cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac
    35521 ataagccagg gttagtgacc tgctttctct tcctgccagc gttcatgtaa ctgattcaga
    35581 cgggtgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct
    35641 ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg
    35701 acggcccctt tacgattatt gcagaccagg atcatatcgc aacccgcatc cagtgatgcc
    35761 tgcccgcgtt cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag
    35821 aaaatcacgc cgtcaaaacc cagttcctga cgcaaaacgg ttttcagcca gtagggagaa
    35881 ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg
    35941 agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct
    36001 tgtggacgtg gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct
    36061 gggaagtgtt tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg
    36121 gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg
    36181 atatgcccga catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg
    36241 ctggccatca gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcccaacagc
    36301 gcagcaaagg attgtgctgc cggtaagcgg gtaaaacctt cgcgaaaacg ctgcacgcgt
    36361 ccaccttcct gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc
    36421 accagttcac gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc
    36481 accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc
    36541 aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc
    36601 atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc
    36661 tcctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg
    36721 ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc
    36781 cacccacacc gccgccagtt ccagcgcgat atcaccatct ccagcatact cccagtcgat
    36841 gagttttaac cctgatgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg
    36901 acttaagcgt aaaggtcgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca
    36961 acccacagtc cgccgcgccg gatcgctttg ctgccagtac agttccagta agggcaacag
    37021 cgttattcgc cagccaaaac gtggttgttg atgtagataa tacagcaagc ctgccagttc
    37081 gttggtatcc ggcaaatacg tttttaccgc gccgggcaga tagtcgacta ccatccagtc
    37141 acggagatat aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata
    37201 ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg
    37261 ctggccctga tgttcaatga gaaaactacc gccactaagc ccactattaa acgtcgtgac
    37321 gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattgct
    37381 gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca
    37441 tttgtagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc
    37501 cgacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc tgcggcgaca
    37561 aacctaactg ttgcttagcc atcgacagtt gctgggcgga aaccagggta aatttcccgt
    37621 tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag
    37681 tacggttgtt aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa
    37741 gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg
    37801 caggcggtgc cgttcgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca
    37861 ccgagggcac tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca
    37921 cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg
    37981 cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcaccccttt acaaatatag
    38041 ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga
    38101 atgtgcggga atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc
    38161 gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt
    38221 tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc
    38281 cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg
    38341 atgtgaacga cagcccacca gcagtaacaa caccagagac accagcccaa agcatccttt
    38401 tctcatcgtt acagaccttt atgcgccagc attggtccca gcggacggcc acccaacaag
    38461 tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtattcat gatcagacga
    38521 tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt
    38581 cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag gatattcgga
    38641 atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca
    38701 tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct
    38761 tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca
    38821 cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt
    38881 cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg
    38941 aatgcgctga aaagacattc tctgtttggt ttacacgaca aatatgaatg cgtatatttc
    39001 tcatttgcat ttgcaagcaa aattattcgc acactaaata taaccgctca tcaccctcct
    39061 ccggcatgag cctgacgaca acataaacca agagatttca aatgctttca acacaattta
    39121 acagggataa tcaatatcaa gccatcacca aaccgtcact acttgccggt tgcatagcac
    39181 tggcactatt accttctgcc gcttttgctg caccagccac tgaagaaacg gtgattgttg
    39241 agggttcagc cacagctcca gatgatggcg aaaatgatta cagcgtaacg tctacctctg
    39301 cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact attgttagcc
    39361 agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa aacacgctgg
    39421 ggatcagcaa aagtcaggcg gattccgatc gtgctcttta ttattcccgc ggattccaga
    39481 tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg aatctgggcg
    39541 acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc gcgacaggac
    39601 tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa cacgcgacca
    39661 gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa gaacggtatg
    39721 tggcggattt acaaagccca ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg
    39781 gctaccagaa taacgactca tggctggacc gctacaatag tgaaaagacc ttcttctcgg
    39841 gcattgtcga tgctgattta ggcgatctta cgacgctttc agccggttac gaatatcagc
    39901 gcattgatgt taatagccca acctagggcg gtttaccgcg ctggaatact gatggcagca
    39961 gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtacaac gataaagaga
    40021 tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga tacctggcaa gcgacactga
    40081 atgccaccca ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa
    40141 acaaagcgga tggtatgctg gttgggccat acagtaatta tggacctggc tttgatcatg
    40201 tcggcggcac cggttggaac agtggcaaac gtaaagttga tgcgctggat ttgttcgctg
    40261 acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc agttacagca
    40321 aacaaaacaa tcgttacttc agttcatggg ccaacatctt cccggatgaa attggcagtt
    40381 tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc ctggcgcagg
    40441 acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt gccgatccgc
    40501 tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca
    40561 tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt
    40621 cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca
    40681 aatatctggc tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga
    40741 atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt
    40801 ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga
    40861 cagtcagtaa aggggtggaa tttgaactca acggcgcaat taccgacaac tggcagctga
    40921 catttggcgc aacgcgctat cttgcagagg ataacgaagg aaacgccgtt aatcctaatc
    40981 tgccacgcac cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga
    41041 cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg
    41101 gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc cgctaccagg
    41161 tgacgaaaaa cttctcgtta caggggaacg tcaataacct gttcgacaaa acctacgata
    41221 ccaacatgga aggttctatc gtctacggcg caccgcgtaa tttcagcatt accggcacgt
    41281 atcaattctg aataacacct gtaaaaaagg cagccatctg gctgccttag tctccccaac
    41341 gtcttacgga ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt
    41401 tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt
    41461 tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa
    41521 tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg
    41581 ccatttcgct ggtacctgtt gcttttgcat cttcagtcgc gtcttcacga cccggcgttt
    41641 tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatc gcataaccga
    41701 taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg atgaagtcga
    41761 tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg
    41821 ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaagaaga
    41881 actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag atcataatac
    41941 cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg
    42001 gcagaccgta cattttgaac aggaagccac cagacagttt gcccgcagtc gggtcacccg
    42061 ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa
    42121 tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc agacaacgtt
    42181 cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga
    42241 aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga
    42301 tggcagccag gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact
    42361 caggcagctt aatacggtag aaacggttaa acatgtacgc tgcaatcgca ccggagataa
    42421 tccctccgag tacgccagta tccgccaagt gtttagaggc gatttcttca gcaggtaaat
    42481 gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg
    42541 cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa
    42601 tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg
    42661 cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggtagta
    42721 cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa
    42781 acataattga gagtgctcct gagtataggt gcttttttta cgttctcacg cgtggcaagg
    42841 ggggagagcc tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa
    42901 ataattcagt gccaatttgt ttgacggcta tcacgtttca acttcagatc attggagaag
    42961 ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gcggttattc accgcccaat
    43021 cttcggcggt gaactttact cgttttagcc attaattacg agctttaaaa aaactcattc
    43081 aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg
    43141 ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt
    43201 cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg
    43261 agtcagtttc caccagtaat cgatccaggg ggacataacg cgcagcatcg cacagttgct
    43321 ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt
    43381 tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt
    43441 tttcttcgcg cagaattgcc agcgtatcgg cacgggcgtc acgggtatgg acgataaccg
    43501 gcttgttcag ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt
    43561 tagtttccgg tgtgtaataa taatccagcc cggtttcacc cagcgctaca acaccctctt
    43621 ctgccgccag acggcgtaaa tcttctacat cgtagggatc attctggttg agtggatgca
    43681 cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag atcccgcata tgtaaataac
    43741 ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca
    43801 gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg
    43861 agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac aacgcccggt
    43921 tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct
    43981 gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc
    44041 gagggagaaa gatggtttgc cagttcggcg accaggcccg gcacatcaac attggtcacc
    44101 tgcgcagcgc catgatggcg ttttagcgca tccatcagca acgttgccac ccagtgtaaa
    44161 cgcgccggag cttgttcatg attaagggcc gccagcagcg aataccagtc gcccgatggc
    44221 acgctatatg ccaacgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac
    44281 aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca atgcggcaag taatgcatcc
    44341 tgtgacattg tcacttcgcg tgaaagccaa gtcacggcgt actgttctgg aggcggcgca
    44401 aggtaatgta accgacaacg actacgtaat gttgccagta aacgttcagg ctcgcgggta
    44461 gccaggaaaa accaggtttc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc
    44521 gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaggcgt
    44581 gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag cgtatttttc
    44641 cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat caactgacat
    44701 ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa ataacggctc
    44761 agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg
    44821 tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg taaccatgga
    44881 taccatctca tgcgtccaac tccttcaccc agtgggtcac ggtagtgcgg attgcatcca
    44941 tcacggcctc cagcggctgg gtggcatcaa tggtatgaat acttttatct tgtgcagcca
    45001 gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa agactcttgc tcaatacgat
    45061 ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga
    45121 gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt
    45181 ggtcaatacc acgtcctccg ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa
    45241 tcacccaggt gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt tgaacgcgcg
    45301 cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct accgatttga
    45361 tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt tcccgagtga
    45421 aaaccatgtc gcggataccc agttgctcga gcgtctcaac caccacatta cgcgcggtag
    45481 ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt tttttcctta
    45541 agcactttca gataatcctg cacagacttg ttatgactgg caagattggt attaaacgtg
    45601 tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca
    45661 gccttcagcg aatccgcccc cggcgtagct atcgcacccg gcggcaaacc ggtaatggta
    45721 taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc
    45781 tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg
    45841 ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc tttttcgata
    45901 attgatgcca tcgtcaccag ctgattttta tctttataag gcagaccgtc cgcacgccct
    45961 tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgag ctcgcttgag taacgcgaca
    46021 tcggtggtat tggcggtata catcaaggtg tctggccaga accaaccttc aatccactcc
    46081 gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc
    46141 ttgatatacg gagcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc
    46201 agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca atttcagcat ctcgcgcacg
    46261 gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata
    46321 cgcagcagcc attgaaaaac ccgtgggcga ttgatgatct tatcagcata aagctgttca
    46381 ccgagcgcca gacgtccggt ccctggcttc agggtaaata tcgtctcttc tttgataagc
    46441 aatttgctgt cggcaagatg gcgaaccttc cagacgccca caccagcggc gatacccagt
    46501 accaccagca ataacaagat aatcaataac acttttttca tgactaattc gggcgctcac
    46561 aaagtggggc taaatattca tataacgttg ctgacgaaaa ggtgacatcg ccacaggcac
    46621 gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct
    46681 cctccagcga ggcttgcact tcgacaagct gataagagga ttgtgccagt aaacggatac
    46741 agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacat
    46801 tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct tcgctgtcaa
    46861 ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcgaatcaat acttgctcaa
    46921 gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta
    46981 gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa gccgtaacgg
    47041 agagaatccg cgttgctggt ccgctgttca atgtgctgta ccctcgcccg ccactaccgc
    47101 gactgatcac gactttcagc acaccatttt gctgttctgc tgccagcgtt ttcatctctt
    47161 gttcaagctg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca tcctgtagtc
    47221 gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac
    47281 aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc ttataaccgt
    47341 taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac
    47401 ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg
    47461 gaaaatgaca acttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa
    47521 ggagttacac agcgtgtatt ccattccgct gacctgacgt gcttcatgcg gtacgaaatc
    47581 cagatcgcaa ccttcatccg ggttatccaa gttgatggtt ggcggaacag cctgatcgcg
    47641 cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca ggtgaccggt
    47701 catagatttc gtggagctta ccaacacacg gcttgcagct tcaccgaaga tggttttcac
    47761 cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc
    47821 aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccattgcca gagctgcgcc
    47881 tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac
    47941 gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt caagtaccag
    48001 cataccggca ccatcgccca gtacgaagcc atcacgctct ttatcccacg ggcggctcgc
    48061 cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc
    48121 cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc
    48181 gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg tcgcgataga
    48241 gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat
    48301 cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg
    48361 gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt
    48421 tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat
    48481 tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca
    48541 gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc
    48601 gattaggctg atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt
    48661 gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt
    48721 cctccaagga gggaaaaatg attctagtgg gacaaaaaga taaaactcag gcggtcgaac
    48781 gaccgcctgg agatgttcac ttacgcctgg tggccgttga tgtaatcaat ggcagcctga
    48841 acggtggtga ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga
    48901 gccattacca gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca
    48961 ttgttggtaa cttcttcctg cttaacgccc agctgttcgc cgataatttt cttaacgcgt
    49021 tcttcgatag tgctcatact cttaaatttc ctatcaaaac tcgctttcgc gatggttttc
    49081 gtagtgtata aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt
    49141 acgttatttt gaggccttac cccccaataa cgcaaatcat tttgcactaa ttattactca
    49201 taaccacgca gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca gaccatgtac
    49261 atcccgccgt tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg
    49321 aatgcaaccg cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc
    49381 aggatacccg cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc aataaagccc
    49441 ggagcaacaa cgtttacagt aataccgcgt gacgcaactt cgcgcgccag tgatttactg
    49501 aagccgatca agcccgcttt cgccgcagcg tagttggcct gaccgccatt tcccatggta
    49561 ccaaccacag aaccgatagt gataatacga ccatgacgct ttttcatcat agcgcgcatt
    49621 accgcttttg acagacggaa aacagatgaa aggttagttt cgataatatc gttccactct
    49681 tcatctttca ttcgcattaa caggttatca cgagtgatac cggcattatt gaccaggata
    49741 tccacttcac caaattctgc gcgaattttt tccagaacag attcgataga tgccgggtcg
    49801 gtcacattca acatcagacc tttgccgttg gcacctaaat aatcactgat cgcctgagcg
    49861 ccattttcac tggtcgcagt gccaataact ttcgcgccac gggctgcgag cgtttcagca
    49921 attgcgcggc caattccgcg gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc
    49981 atgattttcc tcttttaaag ctcgagcgcc gctgccatcg ctgaaggttc gttcagcgcc
    50041 gaggcggtca gggtgtcgac aatgcgtttc gtcaggccag taagcacctt gcccgggccg
    50101 acttcataga gatgttctac gccttgcgct gccatgtact caacagactt cgtccactga
    50161 accgggttat acaactgacg taccagtgcg tcacggatgg catcaccatt ggtttcgcat
    50221 ttcacatcaa cgttattcac aacagaaact gttggtgcgt taaaggtgat tttcgctaat
    50281 tctactgcca gtttgtcggc tgctggtttc atcagcgcac agtgagacgg tacgctcact
    50341 ggtaacggca gcgcgcgttt tgcgccagca gctttacagg cagcgccagc acgctcaacc
    50401 gcttctttat gaccggcaat aaccacctgt cccggagagt taaagtttac cggagaaacg
    50461 acctgacctt ctgcagcttc ttcacacgct ttcgcaatag acgcatcatc cagaccgatg
    50521 attgccgcca tagcgcccgt gccttccggt acggcttctt gcatgaactt accgcgcatc
    50581 tcaaccaaac gcaccgcatc agcgaaatca atcacgccag cgcaaaccag cgcggagtat
    50641 tcccccaggc tgtgaccggc catcattgcc ggtgctttac cgccctgctg ctgccatacg
    50701 cgatacagcg caacagatgc agtcaacagc gctggctggg tttgccaggt tttattcagt
    50761 tcttcagctg gcccctgctg ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct
    50821 tcagcaaacg tttcttcgac aattggatag ctcgccgcca tatcagccag cattccaacg
    50881 gtttgagaac cctgtccagg gaacacaaat gcaaattgcg tcatgtttta atccttatcc
    50941 tagaaacgaa ccagcgcgga gccccaagtg aatccaccgc caaaggcttc aagcagaacc
    51001 aactgccccg gcttaatgcg cccgtcgcgt acagcttcat ccagcgcgca cgggacagag
    51061 gccgcagagg tattaccgtg gcgatccagc gtcaccacga cattgtccat cgacataccg
    51121 agttttttcg ccgttgcact gataatacgc aggttagcct gatgcggaac cagccagtcc
    51181 agttgagaac ggtcaagatt attcgccgcc agcgtctcat caacgatgtg cgccagttcc
    51241 gttaccgcaa ccttgaagac ttcgttgccc gccatcgtca gatgaattga attctctgga
    51301 ttcacgcggt cggcgtttgg cagcgtcagc aattcaccat aactaccgtc ggcatgcaga
    51361 tgggtggaaa tgattcccgg ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg
    51421 ccaaaaataa taatagtccc acgatctgtt ggatcgcagg tgcgcgccag tacatcggaa
    51481 ccgacgacca gagcatactt caccgcccca gatttcacgt attgatcggc tacgcttaat
    51541 gcataggtga aacctgcgca ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc
    51601 aacatgcttt gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc
    51661 acaacgatca ggccaatctg gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc
    51721 gcttcaaagc ccatggttga aacggtttcg tttggcgcgg caatgtggcg ttcgcggata
    51781 ccggtacgag tgacaatcca ctcgtcagag gtgtccacca ttttttccaa atcggcgttt
    51841 gtccgcactt gttcgggcag atagctgcca gtaccaataa tcttcgtata catgtacgct
    51901 cagtcacttt tcggttatat accgtcactt gcaaactgcg agttcgctgg cagcgtcctg
    51961 ctaccgcaga gttccgcttt tgccaccgtc cagcagctca aaaccagctg ggtatacaga
    52021 ttccaggcga gcggcaattc gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc
    52081 aatcgcgacc gcaaaagctc gctgattggc tgcaccatga ctttttatca ccgtgccgcg
    52141 caatcctaac agacaggcgc cgttatactg gtcggggttg aggtgactga atcgcctcgt
    52201 caggctcttt tgtagccaac gctttaataa cagtagccac cacgaccgtt ttttcccttc
    52261 accctgagat ttcagcagag aaaggaacat cctgacaaca ccttccatcg tctttaacgt
    52321 gacatttcct gtaaagccgt cacaaaccag cacatctgtc ttgccagtta acaactcatt
    52381 ggcttcaaga tagccgatat aattgataga agggattgtt ttaagcaccg ctgaggcatc
    52441 ccgaatactg tcaagaccct ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg
    52501 attgggaatt tccaccacct cttcagccag aactgagccc ataatggcaa attgcaccaa
    52561 cattgtgctg tcacaatcga cgttggcccc taaatcgagg accaccgttt tgcccttttg
    52621 ctgatgtggt aataccgtca tcagcgccgg acgctcaatc ccctccaggg gcttgagtaa
    52681 taattttgcc agccccatca gtgccccggt attaccggca ctgacacagg cttgcgctcg
    52741 accttctttc accagctcca gggccatacg cattgaactc ccacgactgg cgcggatagc
    52801 ttgcgaaggc cgggcatcac tggcgataac tgactgcgca ggaataatct gcagacgcga
    52861 acgttgttca aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa
    52921 aagaagagtg agttgcgaat tagaattcag tgcctgcaat gctgcaggca ctgtcacgga
    52981 agggccaaaa tcccctccca tgacatctaa cgccagggtt agacgtgtca aggtatcgtc
    53041 gccgcccggt ttggtctttc cccagttgcc tggggaaaat cctcactaag cttcatcacg
    53101 cagacgcgtg attacttagc gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg
    53161 tggtgacgca ggtgtttttc accagaagtt ttgtctacag acaggctggt gactgcggtc
    53221 agcgcgtcat gggaacgacg catgccacgt ttggaacggg ttggtttatt ctgttgtacg
    53281 gccatggacc ttactcctca attacggttt atccccgatc caacggggag caccaattac
    53341 ttacgcttta agctggctaa tacggcaaat gggtttggct tttgcacttc ttcaggcagt
    53401 tcaccaaaga ccatgtccgc ttcggacact tcacagtgtt cagaatcatg caccggaact
    53461 accggcaagg cgaggatgat ttcatcttca accattgcaa gcagatcgat ttcaccgaat
    53521 tcgttaacct caatcggttc atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc
    53581 acaggactaa aacaatacgt tgtgtagacc tgatgagtaa acggcttccc gcaacgctga
    53641 cactcgagcg ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg
    53701 atagcgaacg acatggagca ttccacatca ctgtccacac tgactacgga ttcggcgacg
    53761 cgctcaacct gatcaggggt atagataccc tggtaatcaa ggcgtttttg agccgtacga
    53821 accggatcga gagtcagggg gaattttacc ttttgcatag ggcgcgcata ttaactttgt
    53881 aacgtcatag agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca
    53941 cattgcgggt tactgcttta ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac
    54001 gaagcgcata gtttaaaatg gctatcatca atacgctata tctgatggaa aaaaatatgc
    54061 ctaaacttat tttagcttcc acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa
    54121 tctcttttga atgtgcagca cctgaggtcg acgaaacccc acgcagcgac gaatcaccgc
    54181 gacagttggt gcttcgactg gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg
    54241 atcatttaat tattggttca gaccaggtat gtgttcttga tggcgaaatc accggtaaac
    54301 cgttaacgga agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct
    54361 tctatactgg actggcgttg tttaattcgg cgaatgggca tctacaaaca gaagtggagc
    54421 cttttgacgt ccatttccgt cacctaagcg aggcggagat tgataattac gtgcgtaagg
    54481 agcatcccct gcactgcgcg ggtagcttta agagtgaagg atttggcatt acgctgtttg
    54541 agcgtttaga gggacgtgac cctaacacgc tggttggttt accgctaatc gcgctgtgtc
    54601 agatgttacg tcgggaaggg aaaaacccgc tgatgggata gttgtttgcg ccggatgcgg
    54661 ttacgtcgca tccggcaatc ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc
    54721 caaccgccca ttatcaggct tatatgcttg tattagcgcg cgttacgcag tttttgcaaa
    54781 caacgcttca aaccttcatc catcggcgct tcgatacgca tcacctcacc ggtccccgga
    54841 tgggtaaact tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca
    54901 gtgagctgtc tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat cggatgaccc
    54961 gcatactgtg tatgcacacg gatctgatga gtacgtccgg ttaccgggct acaacgcacc
    55021 agggtggcaa atgcatagcg ttcttccact ttaaagcgtg tttccgacgg tttgccttcc
    55081 tgactgacac gcacgatacg ttcgccgctt tgcagaatat ttttcaataa cggcgcttga
    55141 acgctcttca catgcgactg ccactgaccg cgcaccagcg ccaggtaatc tttttgcatt
    55201 cctttttcac gtaattgctc atgcagagaa cgcaacgccg agcgtttttt cgctaccagc
    55261 aacacacctg aagtgtcccg gtcgagacga tgaaccagtt caaggaaccg cgcttccggg
    55321 cgcaacgccc gcaaaccttc gataacgccg aagcttaaac cactgccgcc atgtaccgcc
    55381 gtaccggaag gtttattcag caccaggata tgatcatctt catataagat gacgtccgcc
    55441 agcgccgcca ccttttgcag atgtggcgaa accgcctctt cttcccgctc agcaacgcga
    55501 accggtggga tacgcacctc atcacccgct tcgagtttat attcaggctt aatacgtttt
    55561 ttgttcaccc gcacttcacc tttacgcaaa atacggtaaa tcatactttt tggtacgcct
    55621 ttcaattggg tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc ggtgatagca
    55681 acaattttta cggatggagt ctctgttttc atggtgggcg attctaatta gccaacagga
    55741 ttcgcgccac tcatttttct atgcttatat ttagttttgc accttattac ttcactacgt
    55801 gatcacttta ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa actgtgagta
    55861 agcgggtgat aaatggtaaa agtcatcttg ctataacaag gcttgcagtg gaataatgag
    55921 gccgtttccg tgtccatcct tgttaaaaca agaaatttta cggaataacc cattttgccc
    55981 gaccgatcat ccacgcagca atggcgtaag acgtattgat ctttcaggca gttagcgggc
    56041 tgcgggttgc agtccttacc ggtagatgga aatatttctg gagagtaata cccagtctgt
    56101 ttctttgata attgcgctgt ttttccgcat gaaaaacggg caaccgacac tctgcgcctc
    56161 tttgagctga cgataaccgt gaggttggcg acgcgactag acacgaggcc atcggttcac
    56221 acccggaaag gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt
    56281 tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt
    56341 agatgggcag cgtctgtatg acctggatat cgaaagtcca gggcacgagc agaaaaaggc
    56401 aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg cttttgttga
    56461 ttacggcgct gaacgtcacg gtttcctccc actaaaagaa attgcccgcg aatatttccc
    56521 tgctaactac agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga
    56581 agtcattgtt cagatcgata aagaagagcg cggcaacaaa ggcgcggcat taaccacctt
    56641 tatcagtctg gcgggtagct atctggttct gatgccaaat aacccgcgcg caggtggcat
    56701 ttctcgccgt atcgaaggcg atgaccgtac cgaattaaaa gaagcactgg caagccttga
    56761 actgccggaa ggcatggggc ttatcgtgcg caccgctggc gtaggcaaat ctgctgaggc
    56821 gctgcaatgg gatttaagct tccgcctgaa acactgggaa gccatcaaaa aagccgctga
    56881 aagccgcccg gctccgttcc tgattcatca ggagagcaac gtaatcgttc gcgccttccg
    56941 cgattattta cgtcaggata tcggcgaaat ccttatcgat aacccgaaag tcctcgaact
    57001 ggcacgtcag catatcgctg cattaggtcg cccggatttc agcagcaaaa tcaaactgta
    57061 caccggcgag atcccgctgt tcagccacta ccagatcgag tcgcagatcg agtccgcctt
    57121 ccagcgtgaa gtccgtctgc catccggtgg ttccattgtt atcgacagca ccgaagcgtt
    57181 aacagccatc gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg aagaaactgc
    57241 gtttaacacc aacctcgaag ccgccgatga gattgctcgt cagctgcgcc tgcgtgacct
    57301 cggcggcctg attgttatcg acttcatcga catgacgcca gtacgccacc agcgtgcggt
    57361 agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat
    57421 ttctcgcttt ggcctgctgg aaatgtcccg ccagcgcctg agcccatcac tgggtgaatc
    57481 cagccatcac gtctgcccgc gctgctccgg taccggtacc gtgcgtgaca acgaatcgct
    57541 gtcgctctct attctgcgtt tgatcgaaga agaagcgctg aaagagaaca cccaggaagt
    57601 tcacgccatt gttcctgtgc caatcgcttc ttatctgctg aatgaaaaac gttctgcggt
    57661 gaatgcgatt gaaacgcgtc aggacggtgt tcgctgcgtg attgtgccaa acgatcagat
    57721 ggaaaccccg cactaccacg tgttgcgcgt gcgcaaaggg gaagaaacgc cgaccttaag
    57781 ctacatgctg ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag aagagttcgc
    57841 tgaacgtaag cgtcctgaac aacctgcact ggcaaccttt gccatgccgg atgtgccgcc
    57901 agcgccaacc ccagctgaac ctgccgcgac tgtcgtagcc ccagcaccga aagctgcaac
    57961 ggcaacacca gcagctcctg cacaacctgg gctgttaagc cgcttcttcg gcgcactgaa
    58021 agcgctgttc agcggtggtg aagaagccaa accgaccgag caaccaacac cgaaagcaga
    58081 agcgaaaccg gaacgtcaac aggatcgtcg caaacctcgt cagagcaacc gccgtgaccg
    58141 taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa
    58201 ccgtcgtaat cgtcgccagg cacagcagca aactgccgag acgcgtgaga gccgtcagca
    58261 ggctgaggta acggaaaaag cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg
    58321 tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt
    58381 tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg
    58441 tcgtaaacag cgtcagctca atcagaaagt gcgttacgag caaagcgtag ccgaagaagc
    58501 ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa ccaattgttc aggaagctcc
    58561 agctccacgc acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga
    58621 acagcaagaa gagaacaatg ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg
    58681 ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg
    58741 ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc
    58801 tggcaaagtc tggatccgct atccaattgt acatccgcaa gatgtacaag ttgaagagca
    58861 gcgcgaacag gaagaagtac aagtgcagcc gatggtgact gaggtccctg tcgccgccgc
    58921 tgtcgaacct gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg tagaagcccc
    58981 cgtaccggtt gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg aagtgattgc
    59041 cgccgcggta actgaacagc cgcaggtgat taccgagtct gatgttgccg tagcccagga
    59101 agtcgcagaa cacgcagaac cggtggttga accgcaggaa gagacggcag atattgaaga
    59161 agttgccgaa actgctgagg ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc
    59221 gccagtcgtc gctgaagtcg cagcagaagt tgaaacggta gccgcggttg aaccagagat
    59281 caccgttgag cataaccacg ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt
    59341 tccggaggca ccgcgtcaca gtgactggca gcgccctact tttgccttcg aaggtaaagg
    59401 tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc
    59461 tgttgagtaa taattagctc aaagtaatca agccctggta actgccaggg cttttttatt
    59521 tcatgtttga atcaacatat taaaattcat caaatggcat ccttgctaac caacaatgca
    59581 aaataggcaa aaaaaatgcc cgccccggcg atcccagagc gggcagacaa aacataccca
    59641 gtttcatgat atgttttaaa gcgagcttat ttgttgagct ggaagagcga caatccctgc
    59701 atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa
    59761 atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg
    59821 cgatcgctac ctaaggaatc cagcgactcc agtttgttca gctgcgtgcc taattccgtg
    59881 cgaacggtaa ggacattatt cagcgagttt ttcagtccac ggttggtttt atccaatgcc
    59941 gcagcggcga tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg
    60001 gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc
    60061 gcgttactgg taatactgtc gaaaattttg tcgcccgtat gtcctatcac catcgaacgc
    60121 gaagcatcga cctgttgctt aatactttct gttccgccga cataatcacc gttagcctca
    60181 ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt agcgcccatt accgtcagtg
    60241 gtgttcgcca gattcagcaa ctggtcacga agcccctgaa tatccgtagc cagcgaggcc
    60301 cggtcatcgt cactcaaggt gccattgctg gcgtagacaa ttttttcctg agcattctgg
    60361 atagcagtgg tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa
    60421 gtacgtgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aacagcttgt
    60481 gatgcagcaa tgggatcgtc tgaagggtta acgactcgct tacccgtcga catctgctcg
    60541 ccgtacttca tccattctgc ctgagaattg gtgatgccgc gcatgttttg ctggtacatc
    60601 atccgggtac tgaaacgcat tttatctccc cttagcgaat gttaatcagc gcatcaaaaa
    60661 tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca
    60721 gatttccgta ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct
    60781 gcgtcaccac attaccttgc gtggtgctac tggttttcaa cgtcgcggtt ttattaccga
    60841 tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac
    60901 tgttgctttg cagatccagc agggcctgac cgttgcggtt gtcgctatca cccgcatctt
    60961 cttcgctcgc catcgcgatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg
    61021 cgtcacttac tggtttcagc gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca
    61081 actccagacc atcaaatgcc actttaccgt tggcatctgg tgtcgccgta aaagtggtat
    61141 tgctggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg taatctgtcg
    61201 ccagtaccac ggaggcatca gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg
    61261 tgttttgctg aaccgcgggc ttaccgatag taaagaaatc ttcaccagca tcgccgttag
    61321 catcaaatcc ggctttgtgt tggctgttga aagcctcggc aaatgccagc gccagttgtc
    61381 caagcgtatt acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg
    61441 acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacgtaag
    61501 cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac
    61561 ttccctgaac cagtgagtaa ccattggcat cgtgatgtta taagtaccgc catcctgaac
    61621 gctgacttct acgccaacaa tctggtttaa ttcgctcacc agttgatcgc gttgatccag
    61681 cagattgtta ggtgacgccc ctgcccccac gcctgtcagg cgcgagattt gatcgttcag
    61741 gctggcaatt tgtttagcgt aattgttgat ctgatcaacg ctggcaccta tcgcgatatt
    61801 gacctgtttg tcctggtcgc gcagatattg atcggtggtt ttaaactgat tcaccaaccc
    61861 ttctgatttc ccaatcagcg cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt
    61921 ttgcaggctg gtgaagaaat cctgcatctg tgttgccagc gaagaggtac tggtggagag
    61981 catattgtcg attttcgaca tctgctcata gcgggcggtc aggccgctac tttgcgtctg
    62041 cacagcacgt aactggttgg taataaacgt atcatactca cgctgcacac cagaaacgta
    62101 gacgccattg ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg ccataatagt
    62161 ggtttggcgg gtatatccgg caacgttata gctggagata ttattacttg ccgtatttaa
    62221 cgccgcctgg gccgcgttca gtccgctcat ggcgttattg atcaagctgg acatggaggt
    62281 tccttataag ccttcaatta cagagtatta tcggcagcga cccgccggac ttgagttatt
    62341 cagaacagat tatcaatgtt catgctgtag gttttgctca cctggtcgct tatcgatttc
    62401 atctgctgga tcatgttggt gagtttacgg gcatagtgag gatcggtagc atagcccgcg
    62461 tcctgtagga cctgcgcccc ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc
    62521 gggttacgtg ttaacagccc aacgtaatcc gacaatgctt ccagatacga gctgtagacc
    62581 cgaaacttcg cttttacttt cttcgcttcg ccgttttcat attcagtcgt ggtgatttca
    62641 gtgacttgcc ctttccagtt gccagaggct ttgacaccaa acaggttata gcttggctcg
    62701 ccgttttcgc ggcggatttg ccgttgcccc caaccagatt caagcgcagc ctgagcgagg
    62761 atcaaatgat gtggcacacc gctttgctgg cttgccagtt gggcgggcag cgagagttgc
    62821 gcgaggaatg ctctactgtc acccggcagc gaatcatcgt agttacgtgg tacggccttt
    62881 tgcaccagct gcgaaagcgt ctggttttga taacgcacca cggtttcgag cgggaatttc
    62941 atcggtgctg ctggcgtgga ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc
    63001 atcatctctg caagccccag acctttgccc gccgtcatct gttgggcaat ttgctggtca
    63061 tacatactgg tatacagacg agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg
    63121 tcgcgcatgc ttttcaacat catctgcacg aacatccctt ccacctgacg ggccaccgga
    63181 cggatatttg ccgccggatc ttcgctcgct ttcgccttta gttcgttgag tgattgcgca
    63241 tcccaggccg cacttgccag tagtttgctg tcgctgatca tcagatgatt tccagttttg
    63301 cccgcagaca tcccgcactt tgcattgatt gcaaaataga catcagatcc atcggcgtag
    63361 cgcccagcgc attgagcgca cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt
    63421 gcagcgaacc gccgctctgg cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc
    63481 caccaccaaa cggtgtatct ggctggctga cattggcctg acgattaact gttactgaga
    63541 gatttccctg cgctaccgcg cagctgtcga gggtcacttc tcgattcatc accaccgaac
    63601 cggtgcgcga gttaatcact actttagcgt cctgcggggt gacattaacc tgcatattct
    63661 ggatatcggc aaggaaacgg acctgggaac tgttgccact tggtacgcgc acctgaatag
    63721 tccgcgcatc taacgcggtg gcgctgccat atccacgcac gcggttgatg gtgtcagcga
    63781 tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa attaagggta ttcccgacgc
    63841 caaactggct gggcaattca cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca
    63901 gttggttaac ctgaacactg ctaccgcccg cggaggctcc tgcgccgcca accagaatat
    63961 tgccctgcgc cagcgcatac acctgactgt caacgccctt aagcggtgtc atcaacaacg
    64021 tacctccacg caagcttttg gcattaccca tggaagaaac caccacatcg atggtttgcc
    64081 cctgacgtcc aaacggagga agtgacgctg tcaccattac cacagcgacg ttttttagct
    64141 gcatattggt gcccgtcgga acggtaattc ccagctgtga gagcatgtta ttaagcgttt
    64201 gtgtggtaaa cggcgtctgg gttgtctggt caccagtgcc atccagcccc actaccaggc
    64261 catagccaat cagtgagttt tgccttaccc cctgaacact ggtgagatcg cgaatacgct
    64321 cagcctgagc cgccgtcgta accagtagaa gaattaatgc agagagaaat ttaatcacca
    64381 cagcctcact tacattggcg acaggttaag gaagaaacgc tgcaaccagc ccatattttg
    64441 cgcttcgtta atgtagccat tgcctacgta ttcaatacgc gcatccgcca cctgagtaga
    64501 cggtacggta ttgctgccgc tgatagtgcg tggattaacc acgcccgaga agcgaataaa
    64561 ttcggtaccc tgattaatgg caatctgttt ttcacccacc acatgcaggt tgccgttgac
    64621 cagtacctgg tcaaccgtca ccgtcaacgt gccgctaaag gtattgctgg cattggcccc
    64681 gccctttccg ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag cgttaccaaa
    64741 cagcccctgc aaatagcgcg gcacagtatc aaagccaaaa ttagttttac cgtcacggct
    64801 ggcattcgca gaggagcttt tgctggcgct gacgttctcc tgcaacacga tggtcagcgt
    64861 atcgccaata ttgcgtggtc gacgatcttc aaacagcggt tgatagccat agttaatcgg
    64921 ctgagcagac tggaaaatag aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc
    64981 actggtcgcc ccctgcacca gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag
    65041 caccaacaag ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg ctatcatctt
    65101 caaaaatcag taaaccggtg aacctgccac cggttatgcc ttagagttgc gtcagttttt
    65161 gcagcatctg atcggtggtg gacaccgctt tactgttgat ttcgtaagcg cgttgcacct
    65221 gaatcatatt gaccagttct tccgccacgt tgacgttaga cgtttcaaca tacccttgat
    65281 acagcagtcc cgcgccgttc aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg
    65341 tttcggtgta gaggttttcg ccaatgctct ccagtccggt atcattcata aaggtggtga
    65401 gattgagctg cccaacctga accggagctg cctggccttg ttgggttacg ctgaccacgc
    65461 catcacgacc gatggtgata cttaacgcat tcgccggaat ggtgatcgct ggctgcacct
    65521 gaaaaccacc agccgtcacc agctgcccgt tctgatccac ctggaaagag ccgtcacggg
    65581 tataggctga tgagccatct ggcaacatta cctggaaaaa gccctgccct ttaatcgcga
    65641 catctttgct gttattggtc tgcgacagat tgccctggct gtgtaagcgt tcagtggcga
    65701 ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt tcggaagact
    65761 gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg cgctgacgct
    65821 taaaaccgtt agtactgacg tttgccaggt tgttggcaat gacgtccata ttggtttgct
    65881 gggcgtcaag gcccgttttg gcgatccata atgaactgat catataatgt cctgtagtgg
    65941 attgctgaca agcagagcag aagcacgcga cagtgtgaat ttcccctcac cctaaccctc
    66001 tccccaaagg ggcgagggaa tagtccgcgc gcttttattg caatgtcatc aatcgttcaa
    66061 ttaactcatc gacagcagtt ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac
    66121 cttcatctgc atttcaaaac gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc
    66181 attgacgtta ctgccttcca gaacccccga catcacacgc aaggttggat ctgcctgcag
    66241 taccggccct cgcgtggcct gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg
    66301 ctgcacttcg ctgcccgtgg ctttcaccag tttcagacgc cctactggcg caactgtatt
    66361 tgccggatcg cccggattga gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc
    66421 cccttccggc acagcaattg gccccgcctc gcctatcacc ggatgcccct gaattgtcag
    66481 ttgcccggtg ggatcaacct gaatgctgcc attacgcgta tacccttcgc tgccgtcagc
    66541 ggtctgcacg gccagccatc ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc
    66601 atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc
    66661 agtgaaagcc cttccaccgg cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc
    66721 gtagaggcat tggccagatt actggcggtt accgcctgtt gattcagcgt ctggctggct
    66781 gctcccatcg cagtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa
    66841 gttaacccgc gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagactgata
    66901 gttacgctgg gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc
    66961 cagcgcaccg ttggtcaggg tgccaaagtt tcccgtcccg gctgtcccca acagcgccac
    67021 gccagaagat tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt
    67081 ggcaaagttc gccagtacaa tctgccccag cagttgggtt tgttcgttgg aatagttgcc
    67141 gacaaccgta ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccatt
    67201 ctgggtggtt gccacaatgt tgttagcgcc ggtattttgc tgcatggagt tgaggaagct
    67261 cagactaaac gtggcgggtt ctgcaccgtt aattgcgccg gttgcgatat tattcgccat
    67321 cgcaccatcc actaatgtgc cattagcatt aaattccagt gttgtcgctg tcttcgcaat
    67381 gctgtttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    67441 aaaatagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    67501 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcact
    67561 ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt
    67621 cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    67681 cgggtaaccc gtcagctgta aaccttgcat attcaacagg ttacggtttt catccagctt
    67741 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    67801 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    67861 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    67921 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    67981 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    68041 gactgactcc tgaaatgtga aggctcaaat tatctgccgt acttcgtcga gggtggtggt
    68101 gccgtaagtg ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag
    68161 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    68221 attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac gtaccgtccc aggtgaaact
    68281 gtgaactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc
    68341 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    68401 cgtggtcaca gccccttctt cactgccggt tcccgcaaga acagttgtgc cggggatcat
    68461 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    68521 aatagatccg agcgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa
    68581 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    68641 caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    68701 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    68761 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    68821 agcacttcaa cattggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg
    68881 acgacatcaa cgttcggcat ttttacgtag ccctttgcat ctgccagcgg attacctggt
    68941 tcataaacca gtttgtccgg ggcctgactt tcttaacatc ggcaaccttt acgccgcctg
    69001 tcgcagcacc tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc
    69061 catcaggacc ggtcacgcta tcagcatttg ccagattact ggccgccacg ttcaggcgct
    69121 gggactgggc agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt
    69181 aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc
    69241 tcatctggta ttgcaggctg ttatcggcaa actgggtgac ttcctgacgc tgggcgcgca
    69301 gatggagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc
    69361 cgcaggtatc aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa
    69421 taaacgcaaa atgggtcgct atttatgccg ttgatggtca ttgcggacag gtacaactca
    69481 cgttgtagaa atggctgggg gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg
    69541 cgatactgtt cagtccgtta agtacggcga gcaatctcac atcgcaattg cacaactttt
    69601 ttagcgccca actcgcgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca
    69661 atctactacc gccatgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg
    69721 gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc
    69781 aggccacagg aaattatgtg gttgccgcga tgcctattgt gcggggagga aagctggaag
    69841 ctggcaatgt caaactgaaa cgtggacgcc tggataccct gccaccgcgt acggtgctgg
    69901 atatcaatca acttgttgat gccgttagcc tgcgcgatct atcacccgat caacctatcc
    69961 agttaaccca gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg
    70021 ccagcggtga tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg
    70081 cacagaatgc gcgggtgcgc atggtatcgg gacaggtagt cagcggcgtt gttgatgcag
    70141 atgggaatat tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat
    70201 aagcaacaca tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt
    70261 cgcctctgaa gcctgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga
    70321 acagccgggc ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc
    70381 aagcaaaact gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa
    70441 aactggcgat tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga
    70501 tcaacgaagc gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc
    70561 ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg taatggatca tgagcagcaa
    70621 catctctcta tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc
    70681 tcactgctgg cgacgctgga ttacctcgaa cagttacgca agaaagaacc caatacagca
    70741 aatagcgttg atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc
    70801 caaatgaatc aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg
    70861 ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca
    70921 acaccccatc gcggcagtaa aaagatttcg atctgaatat tatctccggc ctgcactgca
    70981 ggccggaatg cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac
    71041 cgccagtgca gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa
    71101 gcgccagggc atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac
    71161 gccagacatc accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg
    71221 cggagtaaag attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc
    71281 cgccagacca atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt
    71341 catcaattgc gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcttg
    71401 gcgggaataa aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga
    71461 gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac gcggtaaatt taccgtactg
    71521 gaacagcgaa acggttaacg gacctgaaag aatgcccaac gcaaccgcac tcggcagtgc
    71581 caacaggaaa caaagacgca acccccagtc catcaaacgg ttgtattcat catgattgcc
    71641 actggcaaaa cttttcgaca gcgacggcag caaaatggtg ccaagcgcca cgcccagcac
    71701 accggaggga aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc
    71761 aagaaacgag gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag
    71821 gatcgccggt cccatctgtt tcaccacgcg cattgctcca gcatcgtgga agttaatgcg
    71881 cggcaggacc agcatgccga tcttcttcag gtgcggtagc tgatacacca gctgcaggat
    71941 gccgccgacc gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc
    72001 aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga
    72061 gaagcggttc cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat
    72121 atagggaaag gtaatcttta gtagctggct ggtcagggca aatttgtcag ctgtgtcagc
    72181 gaagcctggc gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac
    72241 aaccgccagc gcaagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc
    72301 ttcaccctgc ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc
    72361 ttcggcaaag atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt
    72421 tgccatccct gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg
    72481 cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct
    72541 aatccggacc cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac
    72601 ctagattatg gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc
    72661 gccagatctt gtcaacgata cgttgcgcca gcactgccta ttcgccggcg gtttgcggaa
    72721 ctgtctgatt ttgcacgcat tcaatgaagt gccgcgcaca gccgacaaat ccacgtagct
    72781 caagagtgct ctgccaacca ggaatcggtt tattcactac accctgcccg cgctcctcac
    72841 gccattcgcg catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt
    72901 cacgctgact tccggcacgg cgatgcatac aagtggtgat ctgcaaagga ccggccgaaa
    72961 aatggtgctc ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca
    73021 gagaggcttt gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca
    73081 gcaacgtgaa ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta
    73141 gcgaggctgc ggtggcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt
    73201 taaaaccgac catcaacgtc agttttttcc gcgccgccag ttccaccagc ctttcagcat
    73261 cgcgcagatt ttctgccagc ggtttatcga cacagacatg tacccccgca ttgagtaacg
    73321 tactgaccac gtcaaagtgg ctggcggtgc tggagtgcac aaaaaccgca tcacaactgg
    73381 cggcaaggct ggataacgaa tcggcataag gaatgcgcca gctgtcacaa attggcaggg
    73441 ctttggcgcg cgtaggcgac caggctcctt gtaacgtcca gtcagacgct gccgccagca
    73501 ccggtaacca cgctttttgc gcaatgccgc ctaatcccac tacgccgata cgtaattttt
    73561 tcactttaat ctcccagatg ggccagcaac gaatcaagac gttgtttcag ttctgccact
    73621 tcgatttcca gggcttcgac gcgggtctgt aaatcaccgt caaccatgtt cgacatagcc
    73681 gttaccgccg gctgatcttc aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt
    73741 ttacccggtt cgcgggccag acgcaccaca aaaggaccat cttcgcgatt cgccagttgt
    73801 tccagcgtcg actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg
    73861 cgcagttcac ccagcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttct
    73921 gcggcgctca gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg
    73981 acccgattac caaaaccgct cactgtgcgt agataatgac gtttgaccag attatccagc
    74041 tgttcctgca cttcggattc actcagattc atgaccggtt cacggttcgt tttctgatta
    74101 caggccgtga ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt
    74161 tccagcaagc aaccaattac gcgagcttcc agggcagtaa gttgatattt catgagattc
    74221 tccttagcgg ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca
    74281 ttgtccatca atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc
    74341 cagtaaatca ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg
    74401 ttgggtgcgc tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc
    74461 ctgccatttt tgcccaatcg aataaccgag atagcaggca tgaaaagagc cgcgaacaac
    74521 attggaaaaa ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta
    74581 gaaagctgaa ccttgtttat gaaattcgtt aatcatccct agcctggcct gccagcctga
    74641 tggataacag tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc
    74701 tgcgtaataa tccgccagac gccaggcatc acgatcatgc accagacgca cgaccagtcg
    74761 gtctgtggtt aagctcactt ttggcacgtt actgcgatag ccaaacattc tatacctact
    74821 ccttcccgta acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg
    74881 tgaaaagtcc aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca
    74941 aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa
    75001 tgcagaatag aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtatcgca
    75061 ggcgaggaac ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt
    75121 ctttgttgtc ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt
    75181 catggtcggt attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg
    75241 cggcgcaatt gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg
    75301 cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg
    75361 cctgctctcg ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa
    75421 actaatccgt ccacaacaac gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc
    75481 cagtgcggtc attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt
    75541 ctgcgccaca ggggccgttt tgtttgtgct atgtgcggcg ttcaatgcgt ggttgttacc
    75601 ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg
    75661 tgacaagcgt tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca
    75721 agtgatgctg atgctgccaa ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa
    75781 atggatgtat gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg
    75841 gagtgaaaag cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt
    75901 aagcatgatg ccggtgggca tggtcagcgg cctacaacaa cttttcaccc tgatttgtct
    75961 gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc
    76021 ggacgcaaga gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg
    76081 cgctattggt tatatcggtg gtggctggct gtttgacctg ggcaaatcga cgcaccagcc
    76141 agagcttccg tggatgatgc tgggaattat tggcatcttc actttccttg cgctgggttg
    76201 gcagtttagt cagaaacgcg ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac
    76261 ccccccctga ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa
    76321 gctatacatt tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa
    76381 aaatatcccc gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat
    76441 ggtcggtcaa aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag
    76501 catggatatc gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg
    76561 ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt
    76621 gccgcgtttt gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt
    76681 cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg
    76741 tctgattaag aatatcagcg atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa
    76801 cgccgtgaat ggcgaacttt cggaagatga tattcagcta tttccgctac tgcgtaatct
    76861 gacgctggta gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc
    76921 gaaacagaca caaatcaatt tgttatcatc aatggcgatt taatcctgac ccgccgcggc
    76981 agcctgtatt cgcgggttgc cgccgttttc agttttttct gctggcgagt ttatcgctgg
    77041 tgacacatgc tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga
    77101 acaagttttt atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac
    77161 tcacccaata caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt
    77221 tctcaaaaga tatcggttta cccggcgtgg ctgacgccca tattgttctg acaaacttga
    77281 ccagccaaat tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg
    77341 acatgaactc cctgttcggt agtcagaaag cgaccatgaa actgaagctg aaagcgctgc
    77401 ccgtgtttga taaagaaaaa ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga
    77461 cggtacaacc ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttgaaccagg
    77521 cattacgcaa ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg
    77581 gcgaagcaat ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca
    77641 ttccgtttac ggattaatga cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa
    77701 aacatttccg cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat
    77761 taatgactgc accatcccag gtattaaaga tccgccgtcc agacgactgg caccttcacc
    77821 tccgcgatgg cgacatgtta caaactgtcg tgccgtatac cagcgaaatt tatggacggg
    77881 ctatcgtaat gcccaatctg gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc
    77941 gccaacgtat tcttgacgcc gtacctgccg ggcacgattt caccccattg atgacctgtt
    78001 atttaacaga ttcgctggat cctaatgagc tggagcgcgg atttaatgaa ggcgtgttca
    78061 ccgctgcaaa actttacccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa
    78121 ttgacgcaat catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtgc
    78181 atggtgaagt gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa
    78241 gcgtgatgga acctctgcgc cagcgcctga ctgcgctgaa agtcgttttt gagcacatca
    78301 ccaccaaaga tgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca
    78361 ctccgcagca tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc
    78421 tgtattgttt acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg
    78481 ccagcggttt taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca
    78541 aagagagcag ttgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg
    78601 ctaccgtctt tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg
    78661 gcccgcagtt ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc
    78721 aacaggttgc tgaaagcatc gcactgactg atgacaccct ggtgccattc ctcgccgggg
    78781 aaacggtacg ctggtccgtt aaacaataaa aatcgtagct tcctgttgtc attaggttat
    78841 tttacctgta taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc
    78901 atagcgaaaa cttctccatt gccagctggg gctattgacg ccctggctgg cgaactttcc
    78961 cgccgtattc agtatgcgtt tcctgataat gaaggccacg tatcggtacg ttatgccgca
    79021 gcgaataatt tatcggttat tggcgcaaca aaagaagata aacagcgcat tagcgaaatt
    79081 ctccaggaaa cgtgggaaag cgccgatgac tggtttgtca gcgaataata tgcagtgatt
    79141 tttgtttttg ccgggtcgcc ccggcttttt tattttactt acgaattttg agtacaaaat
    79201 ttctacactt cctcaaaata attgcacaat atgctaaaaa aatatgaatg acatgctgtt
    79261 ttattgttca tataaatctt aaaagcagaa atttatctgt tccccccttt aaaaattcgg
    79321 tgaataccct tacttattgg tatactgaag ttgcttcaga taaaaacgca ttgaacctcg
    79381 aataacgttg tctagtaaca cgaattaggg ggccatgatg gaaaaaaata atgaagtcat
    79441 tcagactcat ccgctcgtag ggtgggacat cagcaccgtt gatagctatg atgcgctgat
    79501 gttgcgtttg cactaccaga ccccaaataa gtccgagcag gaagggactg aagttggtca
    79561 gacgctctgg ttaaccactg atgttgccag acaatttatt tcgatattag aagcaggaat
    79621 cgccaaaatt gaatccggtg atttcccggt aaacgagtat cggcgtcatt aacatgacat
    79681 gaccatccac ccaaaaggca cctcaccggt gcctttacca tttctgggtt gtatatctgc
    79741 attcagttaa ttactctgct taaaccatga gagcagagag aaaaaaatga aatacgatct
    79801 catcattatt ggcagcggtt ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg
    79861 tttaaacgtg ctaatgaccg acgcccatat gccaccgcat caacacggca gccaccacgg
    79921 cgatacgcga ttaattcgcc atgcttatgg tgaaggcgaa aagtatgtcc cgttagtcct
    79981 ccgcgcgcaa acgctgtggg atgaactttc ccgccacaac gaagatgatc ccatttttgt
    80041 acgctctggt gtcattaacc tcggcccggc tgactccgca tttctcgcca acgtcgccca
    80101 cagcgccgaa cagtggcaac tcaacgttga aaagctcgat gcgcaaggga ttatggcccg
    80161 ctggccagaa atacacgtcc cggacaacta catcggctta tttgagactg attccggttt
    80221 tttgcgcagc gaactggcga ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc
    80281 gcaactgttc aactgcccgg tcaccgcaat tcgtcatgac gatgatggcg taactattga
    80341 aacggttgac ggtgagtatc aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa
    80401 agacctgctc ccggagctgc ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc
    80461 cgatggccgc tatagcgtga agaataaatt cccggcgttt accggtgaac tgcccaatgg
    80521 cgatcaatat tatggttttc cggcagaaaa cgacgcgttg aagattggca aacataacgg
    80581 aggccaggtt atccattcag cggatgaacg tgttccgttt gcggaagtgg ccagtgatgg
    80641 gtcagaagcc ttcccgttct tgcgcaatgt attgccgggt atcggttgct gcctgtacgg
    80701 cgctgcctgc acctatgata attcgcctga cgaagatttt attatcgata ccctacccgg
    80761 ccacgataat acactgctca ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt
    80821 tttaggggaa atagctgccg attttgcgca agaccaaaaa agcgattttg atttgacgcc
    80881 attcaggctt tcccgcttcc aataatcatc attacggcct ccagaatgag gccgttttta
    80941 caggagtatt taatgcgtcg gctgttgcac tatctcatca ataatattcg cgagcatctg
    81001 atgctttatc tttttctctg gggattgttg gcaattatgg atctgatcta tgtgttttat
    81061 ttttagtttt ccgcactgac ataaactgac aaatatttaa tctccctttc atttagcgtg
    81121 aaaaattgaa tgagattatt cctgcttcta cagcactcac cagacatctc tgatgctgaa
    81181 cccactaaac ttatgaagat tctttcatta ttaattcaaa attattgaaa taaaaataaa
    81241 tatcattcaa tttgttattt ttgtgttgca attcattctc cgacacgcca ttttctttct
    81301 ctcgcaaatt cgtattggat gcacttatgt cattcacaaa tacccctgaa cgctatggtg
    81361 ttatatctgc ggccttccat tggctaagcg caattattgt ctacggcatg tttgctttag
    81421 gtctgtggat ggtcacgctc agttattacg atggctggta tcacaaagca cccgaactgc
    81481 ataaaagcat cggtattttg ttaatgatgg ggctggttat tcgcgttctg tggcgtgtca
    81541 tatctccacc gccgggtccg ctgccgagtt attcgcccat gactcgtctt gccgcaaaag
    81601 ctggacatct cgcattgtac ctattgctgt tcgccatcgg catcagcggc tatctgatct
    81661 caactgccga tggtaaaccg atcagcgttt ttggctggtt tgacgtcccc gcgacccttg
    81721 ccgacgctgg cgcacaggca gactttgccg gtgccctgca tttttggctc gcgtggagcg
    81781 tcgtagtact gtctgttatg cacggattta tggccctgaa gcatcatttc atcgataaag
    81841 acgacactct gaagcgcatg ctgggaaagt cgtcatctga ctatggagta taaaaaatga
    81901 aaaaaagcct gcttggttta accttcgcgt ccctgatgtt ctctgccggt tcagcggttg
    81961 ccgccgatta caaaattgac aaagaaggtc agcacgcctt tgttaacttc cgcatacagc
    82021 atcttggcta tagctggtta tatggcacct ttaaagattt cgacggtact tttacctttg
    82081 atgaaaaaaa tccggctgcc gataaagtga atgtgacaat taacaccacc agcgtcgata
    82141 ctaaccacgc cgaacgcgat aaacatcttc gcagtgcgga ttttctcaat accacaaaat
    82201 atccacaggc aacattcacc tccaccagcg tgaagaaaga cggtgacgaa ctggatatta
    82261 ccggcgatct gacgctgaat ggcgtaacca aacccgtcac gctggaagcg aaattaattg
    82321 gtcagggcga cgacccatgg ggcggtaaac gtgcaggctt tgaggccgaa ggcaaaatta
    82381 agctcaaaga cttcaatatc aagacagatt taggtccagc ttctcaggaa gtggatctga
    82441 ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa tgttgccgga tggcacgcat
    82501 ccggcaatga tatttattct gttggatcag gaatacccag tgttgtattc agacgtccac
    82561 gagacttatt aaagatctta ttgccatttt cacgtcctgc ccgacggcgt cgctgttctt
    82621 ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca acaacctttg tatttttccg
    82681 cgcatactgg acactgaata aacagcaggt ggcagccatc atttttacag ttggtatggc
    82741 tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat ctcatccgat atacgttcgc
    82801 ccatccgctc gtcaaaaaca aaatttttgc caataaaacg caccggcaag ccctgctcgc
    82861 gcgccttacg ggcgtattca ataattccgc cctcgatatg ccagactttg ttgaatccgt
    82921 tatgtttcat ccaggcactg gccttttcac aacgaatgcc gccggtgcag tacatgacga
    82981 tttttttatc tttatgtgcc tgcatcatct cgactgcttt tggcagctgc tcacggaagg
    83041 tatctgccgg aatttcgagc gcgttttcaa agtgccccac ttcatactca tagtggttac
    83101 gcatgtcgat aaatagtgca tcgggatcgt caagcatggc gttcacttcc gccgcttgca
    83161 gatactcacc aacattgctg gcatcaaagt gaggatcgtc aataccgtcg gcaacaatgc
    83221 gatcgcgtac cttcatgcgc agtacccaga aggatttccc gtcatcatcc aacgcgatat
    83281 tcaggcgtaa accctctaaa gccgggtcga aggcatagag ctgcgcgcga aatgtttcaa
    83341 cattgctcgc aggtacgctg atttgcgcgt taatgccctc atgcgccaga tacactcgcc
    83401 caaaaacatt cagcgcggta aacagctgat ataaagcgtc acgggtcgcc ttaggatcgg
    83461 cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg ttcgctctca gccaacattt
    83521 tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac tggcatggtg tacggttcct
    83581 gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa ttatcgataa ttaacatcca
    83641 cacattttac gctacatttg cgcattaaaa attatttgtt atttacaagc gcggcaattt
    83701 cgcccagtct tcagccacaa attttggttg cgggcgaaaa aatgcgacaa tacatacaat
    83761 tgcccgtata ggttgaaaaa caggattgat atgacgaatc tacccaagtt ctccaccgca
    83821 ctgcttcatc cgcgttattg gttaacctgg ttgggtattg gcgtactttg gttggtcgtg
    83881 caattgccct acccgattat ctaccgcctc ggttgtggat taggaaaact ggcgttacgt
    83941 tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg aactgtgctt cccggaaatg
    84001 agcgaacaag aacgccgtaa aatggtggtg aagaatttcg aatccgttgg catgggcctg
    84061 atggaaaccg gtatggcgtg gttctggccg gaccgccgaa tcgcccgctg gacggaagtg
    84121 atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac gcggcatcct gttagttggc
    84181 atccattttc tgacactgga gctgggcgcg cggcagtttg gtatgcagga accggggatt
    84241 ggcgtttacc gcccgaacga taatccactg attgactggc tacaaacctg gggccgtttg
    84301 cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag gcatgattaa agccctgaaa
    84361 aaaggcgaag tggtctggta cgcaccagat catgattacg gcccgcgctc aagcgttttc
    84421 gtcccgttat ttgccgttga gcaggctgcg accacgaccg gaacctggat gctggcatgg
    84481 atgtccggcg catgtctggt gcccttcgtt ccacgccgta agccagatgg caaagggtat
    84541 caattgatta tgctgccgcc agagtgttct ccgccactgg atgatgccga aactaccgcc
    84601 gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg caccagagca gtatatgtgg
    84661 ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt cacgctatta aatctcccat
    84721 gccggatgct tcagaatggc atccggcatt accacagcaa atccccctga tttagcgata
    84781 aaagctctct ggattgcgcc ccctggaagt cgggcgcata attagtgtgt ttatcttttc
    84841 ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg ctatgtcacc ctgtgaaaat
    84901 gacaccccta taaactggaa acgaaacctg atcgtcgcct ggctaggctg ttttcttacc
    84961 ggggccgcct tcagtctggt aatgcccttc ttacccctct acgttgagca gcttggtgtt
    85021 accggccact ccgccctgaa tatgtggtcc ggtattgtct tcagtataat gggttcctaa
    85081 acacatcatt ttagatattg aatatgagac gggcaatcag ctacatcaga ttttcaagtg
    85141 aacgacaact gaaaggggac tctgtacgcc gacaatctaa gctggttact gactggcttg
    85201 ataagaaccc tgagttttac ttggattcat ccttgagctt taaagatttg gggaaaagtg
    85261 cttttagtgg taagcacctg aaaggtggtc taggtgactt tttaaccgct atcgagaaag
    85321 ggcttgttaa agctggtgat acgctactaa tcgagagttt agaccgtctg tcacgtcagg
    85381 atattgatat tgcttctgag ctattacgcc gtatcttaag ggctggtgta gatgtagtca
    85441 cactgagcga tggagaacat tacacacgag aaagtttaaa agacccgtta gccctgatta
    85501 aaagcattct gataatgcag cgggcgcatg aggaaagttt gaggaagtct gagcgtgtcc
    85561 aagctgcatg gaaccgtaaa aaagaactca tctccgaagg tatcaaggta tctaggcgtt
    85621 gtcctgcatg gttaaggctg aatgatgacc gtcgaacatt cacaatcatt cctgataaag
    85681 tggaagttgt taaacgagca tttgacctga gattacaggg tttaagcttt tgggctatca
    85741 ccagaacttt aaacgatgaa ggtcacttgt cattgaatca atacacaccc aaacaaaagg
    85801 ggtggtctga tactgctgta aagaaactac tcagaaatcg tgctgtgatt ggatgtttca
    85861 cacctgctgg acgtgaagaa gttcagggtt actaccccgc aataatcagt gagtcactat
    85921 tctatcgagt acagcaactg aacacagggc aatatggtag ggcttctgta tcaagtaatc
    85981 cgctatcagt caacttgttc aggggtatca tcaaatgttc tgagtgtggg gctactattg
    86041 ttcttggtgg atatgcctta aaacgttttg gaatgtacag atgccctaat cgttcagcta
    86101 acaggtgtag tgcaaaggct atcagtcgta agcaaacgga tgcaactatc ctttacatgc
    86161 tcgcattatg cgaccgtttt gaaactgaaa atactgacac tattgatagc cttaaattac
    86221 agcgtgaaga tttgc
//
LOCUS       DADMFL010000002        82578 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.002, whole genome shotgun sequence.
ACCESSION   DADMFL010000002 DADMFL010000000
VERSION     DADMFL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.002"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..379)
                     /locus_tag="JNZ27_000096"
     CDS             complement(<1..379)
                     /locus_tag="JNZ27_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8662580.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQV"
     gene            complement(450..2564)
                     /gene="fusA"
                     /locus_tag="JNZ27_000097"
     CDS             complement(450..2564)
                     /gene="fusA"
                     /locus_tag="JNZ27_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY8662581.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2661..3131)
                     /gene="rpsG"
                     /locus_tag="JNZ27_000098"
     CDS             complement(2661..3131)
                     /gene="rpsG"
                     /locus_tag="JNZ27_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY8662582.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3228..3602)
                     /gene="rpsL"
                     /locus_tag="JNZ27_000099"
     CDS             complement(3228..3602)
                     /gene="rpsL"
                     /locus_tag="JNZ27_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY8662583.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3728..4015)
                     /gene="tusB"
                     /locus_tag="JNZ27_000100"
     CDS             complement(3728..4015)
                     /gene="tusB"
                     /locus_tag="JNZ27_000100"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY8662584.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNNIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4023..4382)
                     /gene="tusC"
                     /locus_tag="JNZ27_000101"
     CDS             complement(4023..4382)
                     /gene="tusC"
                     /locus_tag="JNZ27_000101"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY8662585.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4382..4768)
                     /gene="tusD"
                     /locus_tag="JNZ27_000102"
     CDS             complement(4382..4768)
                     /gene="tusD"
                     /locus_tag="JNZ27_000102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY8662586.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4768..5490)
                     /locus_tag="JNZ27_000103"
     CDS             complement(4768..5490)
                     /locus_tag="JNZ27_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8662587.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5657..6469)
                     /gene="fkpA"
                     /locus_tag="JNZ27_000104"
     CDS             complement(5657..6469)
                     /gene="fkpA"
                     /locus_tag="JNZ27_000104"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8662588.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6690..6908
                     /locus_tag="JNZ27_000105"
     CDS             6690..6908
                     /locus_tag="JNZ27_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY8662589.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6957..7547)
                     /gene="slyD"
                     /locus_tag="JNZ27_000106"
     CDS             complement(6957..7547)
                     /gene="slyD"
                     /locus_tag="JNZ27_000106"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8662590.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7642..7842)
                     /locus_tag="JNZ27_000107"
     CDS             complement(7642..7842)
                     /locus_tag="JNZ27_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY8662591.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7852..9657)
                     /gene="kefB"
                     /locus_tag="JNZ27_000108"
     CDS             complement(7852..9657)
                     /gene="kefB"
                     /locus_tag="JNZ27_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY8662592.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9657..10208)
                     /gene="kefG"
                     /locus_tag="JNZ27_000109"
     CDS             complement(9657..10208)
                     /gene="kefG"
                     /locus_tag="JNZ27_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY8662593.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10339..12252
                     /locus_tag="JNZ27_000110"
     CDS             10339..12252
                     /locus_tag="JNZ27_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8662594.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12252..13274
                     /locus_tag="JNZ27_000111"
     CDS             12252..13274
                     /locus_tag="JNZ27_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8662595.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNALPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13268..13486
                     /locus_tag="JNZ27_000112"
     CDS             13268..13486
                     /locus_tag="JNZ27_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY8662596.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13540..14409
                     /locus_tag="JNZ27_000113"
     CDS             13540..14409
                     /locus_tag="JNZ27_000113"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY8662597.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14464..14868)
                     /locus_tag="JNZ27_000114"
     CDS             complement(14464..14868)
                     /locus_tag="JNZ27_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY8662598.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15170..15802
                     /gene="crp"
                     /locus_tag="JNZ27_000115"
     CDS             15170..15802
                     /gene="crp"
                     /locus_tag="JNZ27_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY8662599.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15853..17943
                     /locus_tag="JNZ27_000116"
     CDS             15853..17943
                     /locus_tag="JNZ27_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662600.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAAHL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18010..19230)
                     /gene="argD/dapC"
                     /locus_tag="JNZ27_000117"
     CDS             complement(18010..19230)
                     /gene="argD/dapC"
                     /locus_tag="JNZ27_000117"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY8662601.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19316..19879)
                     /gene="pabA"
                     /locus_tag="JNZ27_000118"
     CDS             complement(19316..19879)
                     /gene="pabA"
                     /locus_tag="JNZ27_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY8662602.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19911..20513)
                     /locus_tag="JNZ27_000119"
     CDS             complement(19911..20513)
                     /locus_tag="JNZ27_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY8662603.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTLFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20503..20670)
                     /locus_tag="JNZ27_000120"
     CDS             complement(20503..20670)
                     /locus_tag="JNZ27_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY8662604.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20775..21347)
                     /gene="ppiA"
                     /locus_tag="JNZ27_000121"
                     /gene_synonym="rotA"
     CDS             complement(20775..21347)
                     /gene="ppiA"
                     /locus_tag="JNZ27_000121"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY8662605.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21618..22799
                     /gene="tsgA"
                     /locus_tag="JNZ27_000122"
     CDS             21618..22799
                     /gene="tsgA"
                     /locus_tag="JNZ27_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY8662606.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23061..25604
                     /locus_tag="JNZ27_000123"
     CDS             23061..25604
                     /locus_tag="JNZ27_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY8662607.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSRRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25601..25927
                     /gene="nirD"
                     /locus_tag="JNZ27_000124"
     CDS             25601..25927
                     /gene="nirD"
                     /locus_tag="JNZ27_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY8662608.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26053..26859
                     /gene="nirC"
                     /locus_tag="JNZ27_000125"
     CDS             26053..26859
                     /gene="nirC"
                     /locus_tag="JNZ27_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY8662609.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26878..28251
                     /gene="cobA"
                     /locus_tag="JNZ27_000126"
     CDS             26878..28251
                     /gene="cobA"
                     /locus_tag="JNZ27_000126"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8662610.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28498..28665
                     /locus_tag="JNZ27_000127"
     CDS             28498..28665
                     /locus_tag="JNZ27_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY8662611.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28960..30297
                     /gene="frlA"
                     /locus_tag="JNZ27_000128"
                     /pseudo
     CDS             28960..30297
                     /gene="frlA"
                     /locus_tag="JNZ27_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30318..31340
                     /gene="frlB"
                     /locus_tag="JNZ27_000129"
     CDS             30318..31340
                     /gene="frlB"
                     /locus_tag="JNZ27_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY8662612.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFYDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31390..32220
                     /gene="frlC"
                     /locus_tag="JNZ27_000130"
     CDS             31390..32220
                     /gene="frlC"
                     /locus_tag="JNZ27_000130"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY8662613.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32217..33002
                     /gene="frlD"
                     /locus_tag="JNZ27_000131"
     CDS             32217..33002
                     /gene="frlD"
                     /locus_tag="JNZ27_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY8662614.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33102..33833
                     /gene="phoB"
                     /locus_tag="JNZ27_000132"
     CDS             33102..33833
                     /gene="phoB"
                     /locus_tag="JNZ27_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY8662615.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQLPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33993..35078)
                     /locus_tag="JNZ27_000133"
     CDS             complement(33993..35078)
                     /locus_tag="JNZ27_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8662616.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINLNRSGEETVLRILRESIASI"
     gene            complement(35090..36394)
                     /locus_tag="JNZ27_000134"
     CDS             complement(35090..36394)
                     /locus_tag="JNZ27_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662617.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36406..36759)
                     /locus_tag="JNZ27_000135"
     CDS             complement(36406..36759)
                     /locus_tag="JNZ27_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY8662618.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36770..37648)
                     /locus_tag="JNZ27_000136"
     CDS             complement(36770..37648)
                     /locus_tag="JNZ27_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY8662619.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYCYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37645..38871)
                     /locus_tag="JNZ27_000137"
                     /pseudo
     CDS             complement(37645..38871)
                     /locus_tag="JNZ27_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38872..40035)
                     /locus_tag="JNZ27_000138"
     CDS             complement(38872..40035)
                     /locus_tag="JNZ27_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY8662620.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40119..40481)
                     /locus_tag="JNZ27_000139"
     CDS             complement(40119..40481)
                     /locus_tag="JNZ27_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY8662621.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40498..41403)
                     /locus_tag="JNZ27_000140"
     CDS             complement(40498..41403)
                     /locus_tag="JNZ27_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662622.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41673..42677)
                     /gene="trpS"
                     /locus_tag="JNZ27_000141"
     CDS             complement(41673..42677)
                     /gene="trpS"
                     /locus_tag="JNZ27_000141"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY8662623.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42670..43428)
                     /gene="gph"
                     /locus_tag="JNZ27_000142"
     CDS             complement(42670..43428)
                     /gene="gph"
                     /locus_tag="JNZ27_000142"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY8662624.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43421..44098)
                     /gene="rpe"
                     /locus_tag="JNZ27_000143"
     CDS             complement(43421..44098)
                     /gene="rpe"
                     /locus_tag="JNZ27_000143"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY8662625.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44116..44952)
                     /gene="dam"
                     /locus_tag="JNZ27_000144"
     CDS             complement(44116..44952)
                     /gene="dam"
                     /locus_tag="JNZ27_000144"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8662626.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45059..46345)
                     /gene="damX"
                     /locus_tag="JNZ27_000145"
     CDS             complement(45059..46345)
                     /gene="damX"
                     /locus_tag="JNZ27_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY8662627.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46437..47525)
                     /gene="aroB"
                     /locus_tag="JNZ27_000146"
     CDS             complement(46437..47525)
                     /gene="aroB"
                     /locus_tag="JNZ27_000146"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY8662628.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47582..48103)
                     /gene="aroK"
                     /locus_tag="JNZ27_000147"
     CDS             complement(47582..48103)
                     /gene="aroK"
                     /locus_tag="JNZ27_000147"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY8662629.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48504..49742)
                     /gene="hofQ"
                     /locus_tag="JNZ27_000148"
     CDS             complement(48504..49742)
                     /gene="hofQ"
                     /locus_tag="JNZ27_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY8662630.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49654..50058)
                     /gene="hofP"
                     /locus_tag="JNZ27_000149"
     CDS             complement(49654..50058)
                     /gene="hofP"
                     /locus_tag="JNZ27_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY8662631.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50048..50488)
                     /gene="hofO"
                     /locus_tag="JNZ27_000150"
     CDS             complement(50048..50488)
                     /gene="hofO"
                     /locus_tag="JNZ27_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY8662632.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50472..51011)
                     /locus_tag="JNZ27_000151"
     CDS             complement(50472..51011)
                     /locus_tag="JNZ27_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY8662633.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51011..51790)
                     /gene="hofM"
                     /locus_tag="JNZ27_000152"
     CDS             complement(51011..51790)
                     /gene="hofM"
                     /locus_tag="JNZ27_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY8662634.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51910..54462
                     /gene="mrcA"
                     /locus_tag="JNZ27_000153"
     CDS             51910..54462
                     /gene="mrcA"
                     /locus_tag="JNZ27_000153"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY8662635.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIKGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54627..55187)
                     /gene="nudE"
                     /locus_tag="JNZ27_000154"
     CDS             complement(54627..55187)
                     /gene="nudE"
                     /locus_tag="JNZ27_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY8662636.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55507..57642
                     /gene="igaA"
                     /locus_tag="JNZ27_000155"
     CDS             55507..57642
                     /gene="igaA"
                     /locus_tag="JNZ27_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY8662637.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57707..58375
                     /gene="yrfG"
                     /locus_tag="JNZ27_000156"
     CDS             57707..58375
                     /gene="yrfG"
                     /locus_tag="JNZ27_000156"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY8662638.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58386..58787
                     /gene="hslR"
                     /locus_tag="JNZ27_000157"
     CDS             58386..58787
                     /gene="hslR"
                     /locus_tag="JNZ27_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY8662639.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58812..59690
                     /gene="hslO"
                     /locus_tag="JNZ27_000158"
     CDS             58812..59690
                     /gene="hslO"
                     /locus_tag="JNZ27_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY8662640.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVYSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59825..61549)
                     /locus_tag="JNZ27_000159"
     CDS             complement(59825..61549)
                     /locus_tag="JNZ27_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY8662641.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61928..63550
                     /gene="pckA"
                     /locus_tag="JNZ27_000160"
     CDS             61928..63550
                     /gene="pckA"
                     /locus_tag="JNZ27_000160"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY8662642.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63626..64978)
                     /gene="envZ"
                     /locus_tag="JNZ27_000161"
     CDS             complement(63626..64978)
                     /gene="envZ"
                     /locus_tag="JNZ27_000161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY8662643.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64975..65694)
                     /gene="ompR"
                     /locus_tag="JNZ27_000162"
     CDS             complement(64975..65694)
                     /gene="ompR"
                     /locus_tag="JNZ27_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY8662644.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65886..66398
                     /gene="greB"
                     /locus_tag="JNZ27_000163"
     CDS             65886..66398
                     /gene="greB"
                     /locus_tag="JNZ27_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY8662645.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66495..68816
                     /locus_tag="JNZ27_000164"
     CDS             66495..68816
                     /locus_tag="JNZ27_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY8662646.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69254..69481
                     /gene="feoA"
                     /locus_tag="JNZ27_000165"
     CDS             69254..69481
                     /gene="feoA"
                     /locus_tag="JNZ27_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY8662647.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69498..71819
                     /gene="feoB"
                     /locus_tag="JNZ27_000166"
     CDS             69498..71819
                     /gene="feoB"
                     /locus_tag="JNZ27_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY8662648.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILGVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71819..72055
                     /gene="feoC"
                     /locus_tag="JNZ27_000167"
     CDS             71819..72055
                     /gene="feoC"
                     /locus_tag="JNZ27_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY8662649.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72258..73136
                     /gene="rpnA"
                     /locus_tag="JNZ27_000168"
     CDS             72258..73136
                     /gene="rpnA"
                     /locus_tag="JNZ27_000168"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY8662650.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLRSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73209..73478
                     /locus_tag="JNZ27_000169"
     CDS             73209..73478
                     /locus_tag="JNZ27_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY8662651.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73472..73837
                     /locus_tag="JNZ27_000170"
     CDS             73472..73837
                     /locus_tag="JNZ27_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY8662652.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73838..74626)
                     /gene="bioH"
                     /locus_tag="JNZ27_000171"
     CDS             complement(73838..74626)
                     /gene="bioH"
                     /locus_tag="JNZ27_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY8662653.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPEPVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74664..75347
                     /gene="gntX"
                     /locus_tag="JNZ27_000172"
     CDS             74664..75347
                     /gene="gntX"
                     /locus_tag="JNZ27_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY8662654.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75406..75981
                     /gene="nfuA"
                     /locus_tag="JNZ27_000173"
     CDS             75406..75981
                     /gene="nfuA"
                     /locus_tag="JNZ27_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY8662655.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76342..77646
                     /gene="gntT"
                     /locus_tag="JNZ27_000174"
     CDS             76342..77646
                     /gene="gntT"
                     /locus_tag="JNZ27_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8662656.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNYP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77691..79775)
                     /gene="malQ"
                     /locus_tag="JNZ27_000175"
     CDS             complement(77691..79775)
                     /gene="malQ"
                     /locus_tag="JNZ27_000175"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY8662657.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPSTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79785..82178)
                     /gene="malP"
                     /locus_tag="JNZ27_000176"
     CDS             complement(79785..82178)
                     /gene="malP"
                     /locus_tag="JNZ27_000176"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY8662658.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19798 a        22423 c        20835 g        19522 t
ORIGIN      
        1 ctacctgacg acccagcagg atgtgctcac gagtctgcgg catcgggccg tcagtcgcag
       61 caactaccag gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt
      121 cggcgtgccc cgggcagtct acgtgtgcgt agtgacgggt cggggtgtca tattcaacgt
      181 gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga
      241 atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca gcggtcagag
      301 tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt tttgtacgtt
      361 caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct tcaggagaga
      421 gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat tacggcctga
      481 gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga tgcacgacct
      541 ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg tacttcagcg
      601 tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg acgacggctc
      661 aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac cttcatgatc
      721 ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat agaagcagcc
      781 agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg cagacgaata
      841 cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc ctggatacct
      901 ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatatc gttgatgaac
      961 tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac aacatgacca
     1021 tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt aactttctgg
     1081 cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac gttgaattca
     1141 cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc
     1201 tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc cagacggccc
     1261 agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc gatggagatt
     1321 accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc acacagggtg
     1381 tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc gcgaacttct
     1441 ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca
     1501 gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta aacacggaag
     1561 aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc agagaacggc
     1621 tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag gataccgttg
     1681 atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag catcgcctga
     1741 acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt gttcagaacg
     1801 cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc caggtatttt
     1861 tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg ccattcgtta
     1921 gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc ctggtcagcg
     1981 tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa atgttcttca
     2041 gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt gatctggtta
     2101 acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc aatgcgcgga
     2161 actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac accaccaact
     2221 gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac ttcgattgtg
     2281 aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc
     2341 ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg ttcctgctcc
     2401 tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt atggtttaca
     2461 ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat gtgcgcactg
     2521 ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt cctcgtttat
     2581 cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc gcccgcctgg
     2641 tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc
     2701 atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc agcatcagaa
     2761 agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc agcttcaacg
     2821 atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg tacctgataa
     2881 gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt ttcgagagct
     2941 acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc cagcgcgctg
     3001 tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt tacaaattta
     3061 gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc aatgacgcga
     3121 cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc tacgagttta
     3181 gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta agccttagga
     3241 cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc
     3301 gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc
     3361 aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg
     3421 aaaccgttag tcagacgaac acggcatact ttacgtagcg cggagttcgg ttttttagga
     3481 gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc
     3541 acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc
     3601 attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac gtcctcacac
     3661 aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat
     3721 cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg aaatcagtat
     3781 agtcaatggg aatgatgttg ttcaaaattt gaccagtcaa accgcgggca ataaggtctt
     3841 cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag cggttaccgt
     3901 caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca ctgagcagac
     3961 gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc agcataaacg
     4021 cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc atctgcttcg
     4081 agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc gcgcagtgaa
     4141 gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa agtggcaatg
     4201 taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa aacgccatca
     4261 gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa agcatctaaa
     4321 ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc aattcgtttc
     4381 atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg ccaagtccgc
     4441 ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct gcttcagttt
     4501 catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc acaccatgtt
     4561 gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc ggtgaggtca
     4621 gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac ttatggcctt
     4681 ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta ccgtatgctg
     4741 gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat caccgctctt
     4801 gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt cagcaacctg
     4861 gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca cgatctgacg
     4921 atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg tgaactccag
     4981 cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac cgacatccgg
     5041 ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa gattcatatt
     5101 gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag tcagggactt
     5161 cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc tatccgctcc
     5221 cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa tcttccggcc
     5281 tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca aagagtgcaa
     5341 aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg cttcgtagga
     5401 tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat ccaactcact
     5461 ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc aggagcctgt
     5521 cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat acagcggagg
     5581 ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg cctggtcagg
     5641 cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca gcttccggct
     5701 ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac actagggtag
     5761 aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt ggaataacca
     5821 gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc gggataacac
     5881 cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac tctttaccgt
     5941 cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt gcttcgcctt
     6001 taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca cctttctctt
     6061 tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg tctttttcca
     6121 tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct
     6181 cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg atcagctgat
     6241 ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc atgtaacgac
     6301 ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac gctgctttgc
     6361 tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt gcatgcaggg
     6421 caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc catatctcca
     6481 ggatcggggc atctcacccc agggttaact attatcagaa gggtactata aagcgttgtc
     6541 gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct ttttgtttaa
     6601 attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa gtagaatccg
     6661 cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa gcacgcctgg
     6721 cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg aacgtcacgg
     6781 tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg ctgaccgaga
     6841 agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg cctccgccac
     6901 attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt tcggtattag
     6961 tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc gtgttcatga
     7021 ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg gtgatgatcg
     7081 tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg aatcgccaca
     7141 acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc aaccactacg
     7201 tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt ttcagccagg
     7261 aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg aacacgttgc
     7321 actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac atcaaatttg
     7381 tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga accgtgacca
     7441 tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc gtcttctgta
     7501 cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat atctcctgag
     7561 catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac tcactagctt
     7621 aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt gctcatcttt
     7681 gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc acttaacaca
     7741 ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg cctgacaggc
     7801 cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc tctactcaaa
     7861 ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga aaatctcttc
     7921 cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat gcatcgggat
     7981 taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc gctgtgcctg
     8041 atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg cactggagaa
     8101 tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc
     8161 gcgcactcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt ccaccagctt
     8221 catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct ctgcacccgc
     8281 agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc catatttacg
     8341 catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt tatttgccat
     8401 cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga cctggggttt
     8461 atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact ggcgggatag
     8521 ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg tcaccgtcac
     8581 cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg tagaaaagag
     8641 gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc gctctgagct
     8701 acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg ccaccagcac
     8761 aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt tgagcgacat
     8821 gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga tagccgtttc
     8881 cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg taccgagcgc
     8941 catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca gcagcgtcgc
     9001 ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg gacgcagtaa
     9061 atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga tcttcatcca
     9121 gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg ctgggatcac
     9181 tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc tgcgattcat
     9241 ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa ggccaatgcc
     9301 accgatcacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc
     9361 cgcgcttaac agcacctgtg ccacgcctac gccaaaaatc gaacgccgca gttgccaaag
     9421 tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc cgagttccga
     9481 aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc caattgcaat
     9541 ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca acggcaccgc
     9601 agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac cttccatcag
     9661 cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc gtggctcgcc
     9721 agctcctttg cgctttgccg tctcgcccag taaataatga tggaacttaa ccaatgcatc
     9781 cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg gtagcgattc
     9841 agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt acgccagtac
     9901 tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa tacccggtcc
     9961 agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg aaagacaatc
    10021 acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa atcgggatag
    10081 tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt aagcagtacc
    10141 cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac tttcgctggc
    10201 tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt gggctaccat
    10261 tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag ttaacatagt
    10321 ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc
    10381 gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg cctggtgggt
    10441 aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat cagcgccgac
    10501 ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca ggaaacgcca
    10561 gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata tcgtcaactg
    10621 gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc gaccattcat
    10681 agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag cctgctgcac
    10741 ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc cggtggctgg
    10801 cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct gctgctcgac
    10861 gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg gttgaagagc
    10921 tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc gatcgttgat
    10981 aaaattattc agatcgaaca acaaagcatg ttcgagtaca ccggcaacta cagttcgttt
    11041 gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag ccagcaggaa
    11101 cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac caaagcgaag
    11161 caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc cgcacacgtc
    11221 gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag
    11281 atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat taaattgaat
    11341 ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa atcgacatta
    11401 atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg
    11461 atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga cgaatcacct
    11521 attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg tgactacctc
    11581 ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt ctccggtggg
    11641 gaaaaggccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct gctgctgctc
    11701 gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cagtcaccga agcattaatc
    11761 gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg ttccaccact
    11821 gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat
    11881 tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga agcgccaaaa
    11941 gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt
    12001 gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat ggagaagctg
    12061 aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta tgaccagagc
    12121 cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa
    12181 gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct gctggaaggc
    12241 caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc agcagtgctg
    12301 aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg ccgcgtttgt
    12361 ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc gacggcgatt
    12421 ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg cgtttagtgg
    12481 tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg gttgaggcgg
    12541 cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa
    12601 accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg tttttacgct
    12661 ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg ctcggcggta
    12721 atatgctggc ctgtttgctg gcaaaggaag gcaatgctct cccggttgat gcggcggtga
    12781 ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag ggcttttccc
    12841 gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc aagctggcag
    12901 cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt cgcatccgtg
    12961 aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc gactattatc
    13021 gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg attattcacg
    13081 ccaaagacga tccgtttatg gatcatcagg tgatcccaaa accggaaagt ctcccctcgc
    13141 aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc ggtacattac
    13201 ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg tatctggagg
    13261 cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa atttaattga
    13321 aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac ttgaacagaa
    13381 agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat ggtcggaact
    13441 gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg
    13501 cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa acatccggtc
    13561 attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa
    13621 atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt tcaccgttac
    13681 acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc
    13741 tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat tgaatacggt
    13801 cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc cgtaccctgg
    13861 aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac tgatgtactg
    13921 ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc gcagcatgtg
    13981 gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa actgatccgc
    14041 gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt gcgttcaatg
    14101 gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa cttccagcgc
    14161 gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc gctggatgaa
    14221 agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg gctgctggct
    14281 atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg cggcaaaatg
    14341 gggctggcaa tggaattaat tatgctgctg ctggtgcaac gattgatgga aggaaagaaa
    14401 atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa cgggatagtt
    14461 aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt ccagcatcaa
    14521 cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg ctgcgtcttt
    14581 caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc
    14641 ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac gccctttttg
    14701 caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca ccatttccat
    14761 cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac cagaggcgga
    14821 ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt ttgctactcc
    14881 actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac
    14941 ctaggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc taaaacagtc
    15001 aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca cattaccgtg
    15061 cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa tccagagaca
    15121 gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca tggtgcttgg
    15181 caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca ttcataagta
    15241 cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt
    15301 taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga tcctctccta
    15361 tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc aggaacgtag
    15421 cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg
    15481 ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga tggcgcgtcg
    15541 tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat
    15601 tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc cggacggtat
    15661 gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt
    15721 gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg gtaaaaccat
    15781 cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta gcgcgccatt
    15841 ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta tgcacttcga
    15901 caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga attacgattc
    15961 ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga tacgcctcat
    16021 aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg tagcttactg
    16081 acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg attaacgctg
    16141 gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc tgcatcgctg
    16201 ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga accattgctc
    16261 atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt ctggatctgg
    16321 cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc agattattgt
    16381 gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct gccaccgctg
    16441 ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca aatgcatatg
    16501 ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca ggaggcgctg
    16561 gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca aaaactggtc
    16621 gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc cgctcacctg
    16681 cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac gatggaaaag
    16741 caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt tgggcaattc
    16801 tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc
    16861 cgtgacttac tggccgataa acagcggcga atgccgttgc ttccggcact gaaaagttat
    16921 ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat gttaagcgtt
    16981 gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct gatgacggta
    17041 ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa tcgctccgtg
    17101 ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat tcccgaaggt
    17161 tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt gcgcaaaaac
    17221 tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca actgttgtgg
    17281 ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg ttgtttgatt
    17341 gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac
    17401 gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag cgaggacccg
    17461 caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa cactctgtat
    17521 aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct ggcagatatg
    17581 aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat gaccacgctg
    17641 gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca gtcttgtgaa
    17701 atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag ttctggcgat
    17761 gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg tacgctggag
    17821 cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc gtcgatggca
    17881 tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg
    17941 taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat tttgcctgat
    18001 gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccctc
    18061 atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat ccggtccggc
    18121 attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg cccgaccttt
    18181 gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat cgctaaacac
    18241 atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat
    18301 gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc
    18361 cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag aagcaatttc
    18421 cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg cagaagtcag
    18481 aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc gccccatccc
    18541 gcactgcact tcatcaaaca ccaataatac ctgatgttga tcgcacagct cgcgcaagcc
    18601 ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg gctcaaccac
    18661 caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt taaagggaac
    18721 gtggatgatg tctgccggtt gcggcccaaa gccgtcggaa tattttggct gcccacccac
    18781 cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa ttttggtttt
    18841 gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc
    18901 ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa tcagtttgcg
    18961 cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca gagtttcgcc
    19021 ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg taactgcaat
    19081 gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc tgccctgacc
    19141 ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat cgaaagtcgc
    19201 gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg cataaataaa
    19261 tatgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc ggaaatcaga
    19321 gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt tctggatgga
    19381 actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg cgggtttcgc
    19441 tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc agcgaatggt
    19501 agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg ctatgtgtaa
    19561 tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg ccaaatgcct
    19621 gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg gcatagtggc
    19681 gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt gagatgacaa
    19741 ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca
    19801 gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt
    19861 tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt ttactcagat
    19921 tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag atcccccatt
    19981 gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca gctcagttga
    20041 tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc acttcccacc
    20101 cggaagggat gcagcacgtc gatttcacaa tagtaatgcg ccagccgctc gacgaacttc
    20161 gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag ggcattgccc
    20221 tctttttcga tataagcaaa gtggcagaac agcgtatcgc cctgataaat atcaacttca
    20281 cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat agtgcgcaaa
    20341 tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt
    20401 tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt acgcatgata
    20461 tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct catagtgcct
    20521 cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta aaggcatctc
    20581 gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct cccagagacg
    20641 ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc
    20701 acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg caagattgcc
    20761 cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg ttttgacggc
    20821 acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc ggcaacgtcc
    20881 atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc atggtcaagg
    20941 aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt gtcagcggta
    21001 cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc atttttgatt
    21061 ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg aatcataaag
    21121 ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac ataatcgaca
    21181 aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat gttaccagct
    21241 gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg agaaagagca
    21301 gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag atttccttaa
    21361 caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag aacaaggcca
    21421 tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac tttttattcc
    21481 gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa acatttaaac
    21541 agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc cattttattt
    21601 ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg attagctttc
    21661 tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg
    21721 ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc ctcaacgccg
    21781 gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc
    21841 agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg ttcagccaca
    21901 gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc attactatgt
    21961 cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt
    22021 tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc gccgcgtttc
    22081 tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg gtgtatgtcg
    22141 ctatttttat tctgaccttc agctgtgagt tcccggcact gggcaaacgt gcaccaaaaa
    22201 cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc tctgttgcgg
    22261 cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag tatgccaaag
    22321 gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg atgtcataca
    22381 tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa cgcattctga
    22441 ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga acaccagcac
    22501 atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat accaccatca
    22561 tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac tttgtcctga
    22621 cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt gttgaacata
    22681 gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt
    22741 gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc tctcattaat
    22801 tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc cgctacgtgc
    22861 atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga aatgtcagac
    22921 aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaaa gagtatataa
    22981 aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta ggcggtaata
    23041 gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt aacggtatgg
    23101 tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac tttgatatta
    23161 ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg tcttacttct
    23221 ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca
    23281 tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag gtgattcact
    23341 ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt tcctacccgt
    23401 ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc accattgaag
    23461 acctcaacgc cattgaatcc tgcgcccgtc gcagcagacg cggtgccgtt gttggtggcg
    23521 gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa acccacgtta
    23581 tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc gagcagctgc
    23641 gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcactccag caaaaacacc cttgagattg
    23701 tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc gaactggaag
    23761 tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca acccagtgtg
    23821 gtctggacgt tgctccgcgt gggggaattg tcattaatga ttcctgccag acttccgatc
    23881 cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag
    23941 caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc gaaaacgcct
    24001 ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta ggtggtattg
    24061 gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac gaaagtaaag
    24121 agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt gcggtactgg
    24181 tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct atcgagctgc
    24241 cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag ccgtctatcg
    24301 gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc actaaaggtg
    24361 atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa gctgaaacca
    24421 aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg aacgcggaac
    24481 tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct tattcgcgtc
    24541 aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga
    24601 aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct
    24661 cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat tctaacgaca
    24721 acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt tctccgggcg
    24781 gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtaaa tttaatctct
    24841 acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa gacgatctgc
    24901 cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc tatgcgaaag
    24961 cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt gttggcgata
    25021 gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa
    25081 tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt aaagacgtgg
    25141 gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac
    25201 cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc aaatatctcg
    25261 accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc gcgccgtggt
    25321 tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac gacaaactgg
    25381 ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt
    25441 ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc atcaacagcg
    25501 acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt ccggcaacgc
    25561 cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt ggaaagacat
    25621 ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca
    25681 ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca acattgatcc
    25741 gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg gcgagctgtg
    25801 ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt gcatggaaga
    25861 cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg tgcagctgcg
    25921 cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc tgtaatgacc
    25981 ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt ttatttttta
    26041 aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc tgcgcgcatt
    26101 gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat ggcgggcgca
    26161 tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga cccgtccgta
    26221 cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat tatcgccggt
    26281 tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc gggcagcatc
    26341 agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct ggtcggttcc
    26401 gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt agataccagc
    26461 atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact gttcttcaaa
    26521 ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg
    26581 gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag
    26641 cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca cagcgaagcc
    26701 tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa tactttatca
    26761 ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt
    26821 gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg tttttacgtg
    26881 gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt cggcggtggt
    26941 gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt aacggtgaat
    27001 gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt aaccctcgtc
    27061 gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc agcgacggat
    27121 gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac
    27181 gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga ccgctcaccg
    27241 ctaatggtag cggtctcctc tggcggcacc tctccggttc tggctcgcct gttgcgcgaa
    27301 aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc cgggcaatta
    27361 cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt ctgggagaaa
    27421 ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa agccattact
    27481 gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt
    27541 ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca aattcagcag
    27601 gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct ggtacgccgc
    27661 gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc gcaggaagag
    27721 attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggt
    27781 ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg caacgcgggt
    27841 attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc ctattcgggt
    27901 attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg acacttaaaa
    27961 accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct ggtgttctat
    28021 atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg tatgcctggc
    28081 gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt gattgacggt
    28141 acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct aattattatt
    28201 ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta atttaacccg
    28261 ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa taaaacataa
    28321 ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga cttacaaaat
    28381 taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa cgagcagtct
    28441 gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg aatgtaaatg
    28501 aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac tgcatgtact
    28561 ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca cccggcaatt
    28621 tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc tgacagaaat
    28681 cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg tatttgtttc
    28741 tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat tttataacta
    28801 catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc acattacaca
    28861 acatcatatg ttgttatatt catcatgcat tgtcatgttg ccttttaaat gactgcaaac
    28921 tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca ggaactccaa
    28981 cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt cggctccggt
    29041 atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct tacggtgctc
    29101 gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc ggaactatcc
    29161 accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg aagccgaccg
    29221 ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc attgtcgatt
    29281 atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc gttgctcggt
    29341 aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg ctccgttgaa
    29401 ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt cactatcgtc
    29461 attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac caccactgcg
    29521 attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac cagttggtcg
    29581 tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc cggaaaaacc
    29641 atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac cctgctggcg
    29701 ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac gccgatttcc
    29761 gacgccctga cctagatccc cgcactcggc agcaccgctg ggatctttgt tgccatcacg
    29821 gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc gcggctggaa
    29881 tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca tccgaaatac
    29941 aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt catcttcgtt
    30001 tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa aaataccctc
    30061 accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct gtggcgtact
    30121 ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct ggtcgcctca
    30181 acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat tgctactggt
    30241 ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt gtcgtaatgt
    30301 tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt ctggtcaccg
    30361 aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg gtgcacgcca
    30421 tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc tgcggctcgc
    30481 cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat cttcaggtct
    30541 acgccatttc cggctgggag ttctacgata acaccccgta tcgcctcgac gatcgttgcg
    30601 cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg ctggagctgg
    30661 gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc ccgattacct
    30721 cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt cacctgctgc
    30781 tctgctacag cgtggtgctg gagatgatca cccgcctcgc gccgaacgcg gaaatcggca
    30841 agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta cgcacctggg
    30901 aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt tataccgttg
    30961 ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg atggaattta
    31021 cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg ctggagattg
    31081 tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc cacaccaccg
    31141 aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc gattacgccg
    31201 aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg ccaatggagt
    31261 ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc cgctattacg
    31321 gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat ttacgcaagg
    31381 agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc tattgaacac
    31441 gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg cggtcgcccg
    31501 cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc gctggcgcag
    31561 acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc gtataacatg
    31621 atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct ggcgatatat
    31681 atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc gggctatctc
    31741 acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact gtgtgagtac
    31801 gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga atcgaacgtc
    31861 gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc gcgcctgttc
    31921 agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag ttatttcgac
    31981 aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc cagcgacacg
    32041 cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga tattattgat
    32101 cggggctatg agggttactg tacggtggag ctggtgacga tgtatatgaa cgagcccaga
    32161 ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga tgagagatga
    32221 aaaccctagc gacaatcggc gataactgcg tcgatatcta cccgcaactg aataaagcgt
    32281 tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc atacagccgg
    32341 gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag gatctcgccc
    32401 gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca caaactcagg
    32461 tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg atggccgact
    32521 ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg cacgcggcaa
    32581 tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa cttaccgctt
    32641 tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg catctcgatt
    32701 ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg aaagcgattg
    32761 ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc attgcctggg
    32821 atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac accatgggtg
    32881 ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg acattaccgc
    32941 aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac ggtgcctggt
    33001 aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc ggaaaacgac
    33061 cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct acggaccgct
    33121 actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat atcgcgcagg
    33181 gggtttacca ggccgggcaa cagctcccta ccgaaaacga gctttgtaca caatataacg
    33241 tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc gtactgatcc
    33301 gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc ctgcttactg
    33361 tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag aaagtgatcg
    33421 aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc ggaaacagcg
    33481 aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt attgatagtt
    33541 catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa ggaagctcca
    33601 cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa aagaccatcg
    33661 atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg ggcgaaccgt
    33721 tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac gtctccgaac
    33781 tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat taaccgtagg
    33841 ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt ttacatcccg
    33901 cacgttcccc tcaccctaac cctctcccca aaggggcgag gggagcgttc agtgcggaat
    33961 ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact ctcacgcaaa
    34021 atccgcagca ccgtctcttc accgctgcga ttcaggttaa tgcgaatcgc acaatgttcg
    34081 agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg cgggatttca
    34141 tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc ttcttccagc
    34201 accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg cgcattggca
    34261 atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag caaccgttcg
    34321 gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg caagacttcc
    34381 agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg aatccgacta
    34441 ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag cttgaagcag
    34501 gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt catcaccgca
    34561 tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag cacatctgcc
    34621 agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc atcaggttgt
    34681 tgctcgtcga cgacctgctt cagtgccaac aggtcattga aatcagcagt aataagcgtc
    34741 agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc atgcaccaga
    34801 agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc agtccccgcg
    34861 ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg ctccacccgt
    34921 tgggtaatac gcggttgatt cagacctgga gttaacccta aatcaccgcc agcaagaaac
    34981 tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt ttgctgcgct
    35041 tcagcaagag tcaggctttg caaaggaaac gtcttcatag gagccttcct taagccggaa
    35101 cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt actgccacga
    35161 ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag taaatggcaa
    35221 ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc gaaccgacca
    35281 gcagcgccac ttccatcagc atattcatgg cgttacggat gttatccgac gcattacgca
    35341 ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact tccgccgaaa
    35401 taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag cccaccgcat
    35461 aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg gtggcaatca
    35521 acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta atcagcgttt
    35581 gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag atcgaaactt
    35641 cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc acgatggcga
    35701 tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa agcccgctgg
    35761 catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc cccagcagca
    35821 tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa tagcgcacga
    35881 ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc cagccaaact
    35941 ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc gagcttaatt
    36001 ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta actggcaaca
    36061 ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc aggctgttta
    36121 tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg agcagccagg
    36181 ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga ccaaaagcga
    36241 tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg atcgggtgga
    36301 tgccgtcatg aaaaacagcc gccgagcgat gcgccagaag cgatgtcata cccgtcaggc
    36361 acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt tcagatggtt
    36421 aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg ccactttccc
    36481 ttcagcgatc attttggcga tatgctcgtc tttcgcttta atgcctggtt tggcaatggt
    36541 gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac aatgccgcgc ccgcaccggt
    36601 attacacgcg ccaatgtaat aatctagttg cccggatttc accttcattg ccgcttccat
    36661 gtcgttgtga atgaaaactt caaaacagcc aggagccgtc gcttcaatgg tttttttaat
    36721 ctgctcacgc tgtaagcctg caacgccaat ctttttcatg agtctgtcct tattggaaaa
    36781 attgagaggg attttcacgt aacatcacat ccacatcggc ctgactgaat cctgactggc
    36841 acaattgcgg aataaaggtg gtcaataaat agtcatagca ataaccacca ttggctttta
    36901 aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg gttcagcaac ccacggtcac
    36961 gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta gtaactgttc ttaccaatgg
    37021 tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa aatgttgtcg aggttgtctt
    37081 tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac tccgtgggct tgcaacagag
    37141 ccagttgttc cagccccatg gtgctgaacg acgtatgcgt ggagatcggg cgtccggtct
    37201 ggttatgcgc cagcgcagcg gcaataaata ccttctcttc cagcggcgta atctttcctt
    37261 cgctggagcc gatctccgcg atgatcccgg ctttcagctc cgtgccatcg ataccctgtt
    37321 caatttcatc gaccatctcc tgcgccagtt cctgcacgct gcgggtcacc acatgttccg
    37381 ggaaaaacgc atcctggtaa taaccagtac aggccaccac gttgatcccg gtttcgcgca
    37441 ttacatcgag cataaattgc gcattgcgcc ccatgtaacg gttggtcatc tcaatcacat
    37501 tacgtacacc ccgggtcatc aggtcgttca tctcctggca aatgaacgca tactgatcaa
    37561 ggcggcagtc cacgttgttt ttaaagccgg agagatcaat atgcagatgc tcatgggcca
    37621 gggtgtaacc cgtcggatca aaactcatag ggtgtctcct gcaaaccgga gggaggaaag
    37681 aaaagagcga ccattttgtg gcggtggcgc acggaaaaat tcacacacgg tagctcccac
    37741 atcagaaagc gtggtgcgca caccgagctg cgtggcgacc agcccttgct gataaaccag
    37801 cactggcacc acttcacggg tatggtggct gtgaccaatg gtcggatcgt taccgtgatc
    37861 cgccatcacg accaggcaat catctggctg catcgcctca acaagccggg caaggttacg
    37921 gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct tctgcatgac cagcgaggtc
    37981 ggtttcctag atattgatgc aaataaacgc cgtcggatgg gtgttaaatt cgtcgagggt
    38041 gatatccata atccgctggc tatccaccag gttttgccag ctcacaccat aaggattgct
    38101 gacgatatct gccaccttac ccaccagcac ggttggcacg cccgcttcat acagtttttg
    38161 cggcacctgc actttttcat caacaccgta gcccatatgc acgacctgga agccgttgtc
    38221 ataagcgcca gaacgcggcg cattaatacc aataaagcgc ccttctttgc tttctgcggc
    38281 atcgagaata cgctgactgt tggttaacag gccgccaaat gtaatgaccc gaccgacctg
    38341 tacctggtca cgcacgatac gaccaatttt gattgcgtcg tcaaaagaga tcacagagag
    38401 attggcggta atgttataga cttggcctaa atccgcctcg agattatcgc caatcgcaac
    38461 cgcctgattg acccacagaa attgcagatc atcgccacgg cgctccacct gccagccagc
    38521 ggaaactaat gcctgctcaa cacggtcaat cacatcgcga aaaggcatcc gcagcggcgg
    38581 taacgggcgc gtgcctaaaa tttcctgatg ccccataaag gtatcgccac cttcatgttg
    38641 cagctctgcc acgccccagg ttgcggaatc tgacggctgc atatcgcctg gcgcataacc
    38701 caatgtgttg attagcccca gcttctccaa cgttggtagc tgcaaatgcg gcaactggct
    38761 caggatgtga ccacaggtat tcgccccggc atcttgcgga cgcaccagcg tgacatcttt
    38821 cattgcccct acgccaaagc tatcaatcac taacaccaca aatcgcgcca tttatgcctt
    38881 cagagggttg ccgaggctgt cataacgacc aactatttct ggttcgccgc gctgaatacc
    38941 ggacaccagc accacatcgc tacgggtgac aaaaatctgc gtgcgaaaac agagcaccac
    39001 tgcactgctc accggatact cgcctgccag cggcagggtg tagtcgatac tgctgtcatc
    39061 cacagttttg agattggttt cagtaatctt ttgattttct ggcgtaaaca ccagcgcatg
    39121 ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag tagctgtcgc cacggaaatg
    39181 atgggagatt tcacttaacc agagcatcgc gatacgttca ggctgatcgc cctgctggtt
    39241 tgccggaata gtaccggtca gcgcatgacc gggttcggca tgagtcgcac cgtattccgc
    39301 cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg ttcagttgct caatcgcaat
    39361 accagatttc gccagttgat cccgtgcttg taccagcgtg tgaagattcg gtgttggcaa
    39421 aactttcccg gcagcctcat cccaaagcag gcaagggaaa tgggttagtc cggctaaatg
    39481 cagccccggc agtttcttaa tttcagcaat cacctcgtgc agcgaatgct gtacaaaacc
    39541 gctctcctgc cccggataaa gaaaatcatc gtcgctataa actttgagca gcacaggctg
    39601 gacccgtccg gccttaaccg ctgccgcaga aacttcccgc gctttgtcga gagtaaacac
    39661 ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc tgatgacaag ggatttgtac
    39721 cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc atgactcgcg cctctttgta
    39781 atccaccgcc acaatgccgc tgtagcctaa tgccagcaat ttttccgcca gccacggatt
    39841 acgaccaaat tgcttggtca tcagatacag ttcaatgccg taaagccgcg ccgtctcaat
    39901 gagccgctta ccgttttcca gtatctgatc cacgtcgatc acccagctgt ccggggcgat
    39961 tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc gccgggttct ggcgtttcaa
    40021 tgcttctaca aacatcttgt tgcctcgttt ctgttccttg aattaaatat tcattttttt
    40081 aaatatttag gttgtgcgac gtggatttgt cgggagcttc aaacctcttc gttagcagcc
    40141 atccataatc cataaagatt ggccagaaaa tagccttctt cacacgggtt aacttccaga
    40201 gcgaattcct tcaacaacac ctgatgcaat tgcacaacgg cttgccagtg gctggattgc
    40261 gccagttccg ccagcaactc gttatccagc ggctctattt cttcaccacg gcgactgcgc
    40321 atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt gctcactgcg caccggcaga
    40381 tggcactctt tttccagtac gttgacgacc tgcatcatgc ctttgcagac gtccttatca
    40441 ataacgcctg cctcgcaaag caggttgagt ctggtttcca tatcagatgc ctttcgttta
    40501 ataactgggt tcctgttccc cactcaccac ccgttgttga tgggcaagca gggcgtgttt
    40561 atctacaacg gcacgcagca attgttgcat tgggtaatca tcgactcgcg tcaggaccac
    40621 ggcttcggtg gccagtaaaa atcgcggatc gtctgttagc ggcgtcgcct ctaatcccag
    40681 catggtcagt tcgttttccg ccaccacgtt ccagatcacc gcatcgacat cgcctttaac
    40741 aatgcgttgt aaactctcgt gataagagag atcgactcgt tccacatcac tatcgccaaa
    40801 aaagacatcg gtcatgattt tctgatccgc cgaacgacta tcgagaccta ctcgcttaac
    40861 atttccagac tcacctttgc ggcatatcag ttgatgttca ccaacatacg tatgcgggcc
    40921 aagctcgagg gcgatgcaca aattgttttg gctgagataa ctttccgccg ccaggcggga
    40981 aactactgcc atgtcataga caccgttaag taaacactcc acgcgaatat ccgcgccgcg
    41041 catatgcgca tagtaaaaag gaatgccatc aaactgggct ttcaatccgc tcgctaaacc
    41101 ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc acgttgttga tatccacatg
    41161 actcagcaat gctttgttat ccatctcgac aagataactg ccattgcgcc cacggcgttc
    41221 aatccgtatc gctccgcttg attccagcgt tttcaacgcg gcctgcgtca ggccaaccga
    41281 tgaacggcat tcagttgcca gctcatctat ggttttcagt cgattaccgc atttttcacc
    41341 caacaaataa cgggccagtg tcgtgatgac gacgccttct tttttgataa acgttcgacg
    41401 cataatatat tttcaataaa atgaatattt atatcttcat taaaatgaag ataagtggcg
    41461 acgacgagat gtggaaacgg cgaggcgctt cacatttttt cgggttgttg cgaaaagagg
    41521 ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat tcgacaatgc
    41581 gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc ctctccccaa
    41641 aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa accaatcgct
    41701 tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc gccgtctttc
    41761 atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc ctgcaattca
    41821 gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata catcttgcct
    41881 tcgaactgtt tttccagttc tgggatgctc tggcccgtta ccgcagaaag gatatccagc
    41941 aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg ctcgtcggag
    42001 tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag caggccgata
    42061 acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag cagcgacatt
    42121 acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc gccatacagc
    42181 gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg gtcttcaccc
    42241 accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac cggatagtca
    42301 aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa ctgcgtcatg
    42361 cgactcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg cgcatgttct
    42421 ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca agccagatac
    42481 aaggccagcg tatccaacgt cgctttacgc agcttctgtg catcctggcg cacggtgatc
    42541 gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt tacccactga
    42601 cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc gccactaaaa
    42661 acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc ctaatgcggg
    42721 cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga tagcctcgcc
    42781 gtagttatat ccgtaggtta agccaactga tgggcaacct gccgcttttg ccgcctgaat
    42841 atcattgcgt gagtcgccga caaacagcat ctgttgtggg gcaattccca tccgctcagc
    42901 caccagtaac agcgggtccg gatgcggttt tttgttttgc acatcatcgc cgccgataac
    42961 cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga acggcgtcgg
    43021 tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca acgtatcggc
    43081 aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc gatcgaacag
    43141 tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg gcggtttccc
    43201 cattgtttta cgcagagtcg cacgttcctg acgcgcccag gtcaatgcgc gctccatcag
    43261 aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga cgggcaactc
    43321 cagcgcatac agcgccatat ctaccgcagc agcaagacca ggagcactgt cgaccagcgt
    43381 accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga cttacctttg
    43441 ccagttcact gcgcatttca tcaatgactt ttttatagtc tggctggtcg aagattgccg
    43501 aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg ttcaccttca
    43561 cgccaccgtc cacttccaga cgaatgtcaa agccagactc gtcgatacgg cgacgtactt
    43621 cgcgcagttt atccagtgtt tgaggaatga aagactgacc gccgaaacca gggttgacgg
    43681 acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc agaggtgtcg
    43741 ccgggttaaa taccagaccc gctttacagc cattttcttt aatcagttgc agcgtgcggt
    43801 caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg gcagcgaaat
    43861 caggcacaat gcgatcgacg ggtttcacca tcagatgtac gtcgataggg gcggtaatgc
    43921 cgtagttacg caaggatttc agcaccattg gcccaatcgt cagattggga acatagtggt
    43981 tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt gcggtatctt
    44041 cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac tgtttcatcc
    44101 gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt acaaagccag
    44161 cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc gaactttgac
    44221 gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt gattggagat
    44281 cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat gcgcttgttg
    44341 ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag acagcggtgc
    44401 ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgctccatgc tatcggcgta
    44461 agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca actctgcttc
    44521 cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc gcagattgta
    44581 acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg cccgacggaa
    44641 cggatcctgg cttttgttga actcttcgcg gaactgatag taaacctcgg cgcaatttgt
    44701 ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca tcttcacaat
    44761 gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag aaaagtcggt
    44821 gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc ccttgggcaa
    44881 atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca aaaaagcgcg
    44941 atttttcttc atgccgacta actaattaca ccttctccgg ctgtggagaa agctccgaca
    45001 gcatccagcg cttcaggcag ctcccgcaac gataaaccat tgcgggagat aaccttgatt
    45061 acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg gcctgaacat
    45121 ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac acgccagaaa
    45181 ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag tttttcagat
    45241 tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc agctgcagag
    45301 tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc ttccctccag
    45361 cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc gtagtcgccg
    45421 tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt cgtgctcgcc acaggggcag
    45481 ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ctccgttttc acggttgctt
    45541 gcggtttttt tggttcaatc accgcctgct gacgaactgg gcgcgtagtg gacggacgtt
    45601 cagcggtttg cgttttcgcc gtgtcacgcg atgcattgcc attgcgaaca ggcgcaaccg
    45661 ttgcgggttc agtcggcaac gtggaattga ccgccacatt gttcagctgt tgctgatttt
    45721 gtggctgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt tgaccatccg
    45781 ttgccaccgg ggtttgccct tgagtcggcg tagaagagat cggtggcaga gaaacatcct
    45841 gctgagtatt ttccgcagac gtggttcccg gcgctggctg cacgccattc gcctgatcgg
    45901 tcgcattgcc agcaagatca atactcttct cgccagacgc ggtttgatcg ctggaagtgg
    45961 tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga accagaatgc
    46021 cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt ttgcgcttac
    46081 gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg tcaatttctt
    46141 cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg cgatcgtcag
    46201 tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca gtacgctcag
    46261 aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg ggtttcagct
    46321 cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg cttaccacgt
    46381 tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc ttgttgttac
    46441 gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc gctgcgaact
    46501 tcactcttac caattgccaa cggaagaatt aagcgcatct ctcccgcaag gactttcttg
    46561 tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc attgaccggt
    46621 aacccagccc gcgtgagcag ggttataata cgctgcgttt cggcagaact aaactgcccg
    46681 agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc accatgtaac
    46741 caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc cagattcagt
    46801 aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt cagttcacaa
    46861 caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc atccagattc
    46921 tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat gacttctgcc
    46981 agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc caccaccact
    47041 gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt gaccgcagtt
    47101 ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg aatgaaacga
    47161 acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac gccgccgcca
    47221 agcgccacca gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt aaagacggta
    47281 tcgagtacag ctaggctttt atactgctcg ccgtcaggga ggataacgct atcgacgtta
    47341 acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc cagggtttcg
    47401 ctggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc tggttcatta
    47461 aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac gacaatcctc
    47521 tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata taaagccaga
    47581 attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg ctttgatcat
    47641 cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca ttggccaacg
    47701 cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt ttatcacgct
    47761 gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg ccacgagcgg
    47821 aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc agcacaatac
    47881 cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag ccttcttcgc
    47941 cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc tcttgatcgg
    48001 aatcgtaaaa ttccatattg agtttttgag ctaactggcg cccaatagtg ctttttccgg
    48061 cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg gtactactaa
    48121 gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca tgaactgaaa
    48181 cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag cgggtttggc
    48241 aactgaataa atcacccatc cctctgcata tctggtcgct gcaagcgcac tgccttgcta
    48301 ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag caaaatgccg
    48361 cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac tctcaaatcg
    48421 gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt tcagcataaa
    48481 aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt gataaacacc
    48541 actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc gaaccaggga
    48601 atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt aaaaatgccg
    48661 cccagcgcca acgtttctcc gctttttacc tcgacctgcg tttcgatctc ctgcttatca
    48721 atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt ctggctgatg
    48781 tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc catccccagg
    48841 acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac ctgatatgga
    48901 atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag acgcggactg
    48961 gcgataatat ccagctgctg tttttgttcg agcgcggaaa gctcaagatc cagcaagcgt
    49021 ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac ggagaggtcg
    49081 ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc cagcgtccat
    49141 ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc cgacagctca
    49201 acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct taacgccgtt
    49261 ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact ccctttggca
    49321 ctcagtagct tctcccccgc tttcgccagt tctcccgcgt cggcgtattg cagggttata
    49381 ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc ctggcgggcg
    49441 atattgtcat tctgccaggc aatggaatgc actgagagaa tgttgccttc ctgccgcgtt
    49501 atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac atctgttaga
    49561 tgtaacgaca ccgtaccgct gacgtctggc gacacgacca ggttcaactt ctcctgttca
    49621 gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt cactttttgc
    49681 ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat ccattgcttc
    49741 atttgtatct ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg tcccggtacc
    49801 cagcgttagt acgtctggcg tcagctgtaa aattgtccag ccgttttcca gcacatcgtt
    49861 ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga tgcgctcgcc
    49921 tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc atagatcttc
    49981 cggcgattta aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa
    50041 ccagcgttta accctcatgc ggcatctcca gttgtagcgt gaacaaaaga tcatcacctt
    50101 ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg
    50161 acggcactgc ttcccacagc gttttcaacg ccaactcgcc tccctgcgcg gatggatgcc
    50221 aggaaaccag ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc agcgtttttt
    50281 cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc tggtgatgac
    50341 ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgat agaaaaatga
    50401 gcgtaactaa catcagcaac cagaatgccc agcaaaactg gcggaggcgg ggtgatgtgg
    50461 cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat
    50521 tgccagcgtc cctgcgcatc ctgttgcgtg gctccccgct gattgagatg aaaagacgca
    50581 tcctggcgga gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt cagccccttg
    50641 atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgctc gggtaaaagc
    50701 gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgccgt
    50761 tgcgaacgct gttcgcgcaa ctgctgttgc tccagcaaac gtggcttcgt tatctgtaag
    50821 ctgcgggcga gttgttgttc cgcctgaagc aaaactgcgt ctatgcgcgc ttcggcgctg
    50881 cctgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc
    50941 agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt
    51001 ggcgggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac
    51061 caggcggcgg tagcggcggc tgacgaacag aaacggcctc ccaggggtca aatccgcctt
    51121 cgccacatat cgcgacgctg tcgggatcaa cggataacgc tgccgccagc tccttcgcgc
    51181 tagtcatccc caccgccagt ttgcgccccc agctatagcg cgtcgcccac agccactgtt
    51241 cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta
    51301 atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct gccagcgtaa
    51361 gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg ctgagtgagt
    51421 cttcgctgta atcgaagcgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc
    51481 ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc ggaaatgacc
    51541 gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc
    51601 gactccacgg taacaacgtt ttagccagct gctgcgcatc aacaatccgc ccatctttga
    51661 taatgtcgtg cgccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc
    51721 gcacgatcgc aaccgctgcc gcttcttgct gttgtaaatg caaaccaatt tgccagatct
    51781 taaatgtcat tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc
    51841 tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg
    51901 aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg
    51961 ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggtg
    52021 acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg
    52081 attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccaccggag
    52141 atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac
    52201 ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg
    52261 gcgagtacca ttacccagca actggcgaga aatttcttcc tcagtccaga acgcacgctg
    52321 atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct gacgaaagac
    52381 gaaatcctcg agctttatct gaacaagatt taccttggtt accgcgccta tggtgtcggt
    52441 gctgcggcac aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggcg
    52501 gtgatagccg ggttgccgaa agcgccttcc acctttaatc cgctctactc aatggatcgt
    52561 gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta tatcacccag
    52621 caacagttcg atcagacacg cactgaggcg attaacgcta attatcacgc gccggagatt
    52681 gctttctctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc
    52741 gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag
    52801 gccgcgcagc aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc
    52861 ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc
    52921 gatacgctga aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc
    52981 aatcctcagg gagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa
    53041 ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa
    53101 gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg
    53161 tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaatggt
    53221 gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc
    53281 caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat
    53341 aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt
    53401 gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc aattcgctta
    53461 cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gtgcaatgcg ggcgatgggc
    53521 gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac
    53581 accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac
    53641 gcggtcatgg cgaacggcgg cttcctagtg gacccgtggt ttatcagcaa aattgaaaac
    53701 gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt
    53761 ccagtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat
    53821 gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca
    53881 aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact
    53941 ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga accaggctgg
    54001 cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc
    54061 ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc
    54121 tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga
    54181 gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg
    54241 gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgcctccg
    54301 ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc
    54361 cgcgaagagt atttcatcaa aggtacgcag ccgacacaac aggcggtgca cgaggtggga
    54421 acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag gcgcttcggc
    54481 gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg aacgcgttat
    54541 ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa ccccataagc ctgataagcg
    54601 ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc
    54661 acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc
    54721 atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc
    54781 gattccgggt agagatcttg cgcgaccacg atattcattt tactggaaaa gtaagacggt
    54841 gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt
    54901 agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaatcc tttcgaaaaa
    54961 cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc
    55021 acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc
    55081 cgcacgccat tgctgaactc cagatccaca ctctcgacgg taaacagtcg ggaacgggct
    55141 acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgttcatcgt gggatctatg
    55201 ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg
    55261 ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag
    55321 gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc
    55381 ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc
    55441 gcagaatcat gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg
    55501 gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg
    55561 ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg
    55621 gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag
    55681 agcctgacgc aggtattaca ggtgcctggc ccaacgggag ctagcgcggc accgatctct
    55741 ctggtgctga atgccgaaag caacaacgtc atgatgctga cacacgctat cacacgttac
    55801 ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac
    55861 ctgcccccct tctgggaaca gtacatcaac gatgagaata ccgttgaact gattcatacc
    55921 gattcgctgc cgttggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa
    55981 acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag
    56041 agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt
    56101 cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc
    56161 tgcctgatta ccccggatgt atttgttccg tggctggcag gcggcgcatt actgctgctg
    56221 ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg agaaattcat
    56281 tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc
    56341 aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt
    56401 gctcaggatc tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cgtagtgcgt
    56461 caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg
    56521 cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt attctggatc
    56581 ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca gaccattgaa
    56641 gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt
    56701 agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca
    56761 ccttttttac catttgactg ctcgcagatc atctggaatg acgcccgctc attaccatta
    56821 ccagaatctg aacttgtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg
    56881 caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc gattcaaaaa
    56941 tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc ggatttatgt
    57001 tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa
    57061 gactgggacg cgctggtaaa acgcgccaac gctgggaagc tcgatggcgt gaatgtgtta
    57121 ttacgcccgg tgagtgcgga atcgctggat aacctggtgg caacctccac cgcgccgttt
    57181 atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc
    57241 ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt
    57301 tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg
    57361 catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg
    57421 cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc
    57481 acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca ggcctggcgt
    57541 cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga aagctgcctg
    57601 aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt
    57661 tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat
    57721 tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc
    57781 cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt
    57841 tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct
    57901 aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac
    57961 caccgagatg ggaccgcgcg ccgtactgcg tgaagatacc attccgtttc ttgaggcact
    58021 gaaagccagc ggtaagcagc gaattttgct caccaatgct catccgcaca acctggcggt
    58081 aaaacttgag catacgggtc tggacgcaca ccttgattta ttactttcca cccacacatt
    58141 tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa
    58201 agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt
    58261 tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg agaaacagta
    58321 tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag
    58381 acgccatgaa agagaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc
    58441 gtttttataa aacccgcgcg ctggcccgtg aaatgattaa aggcggtaag gtgcattaca
    58501 acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc
    58561 agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca
    58621 gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg
    58681 cgatggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa
    58741 aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa
    58801 gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aattttgccg
    58861 ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag aaccacgatt
    58921 atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg
    58981 ctacgctgaa gtttgatggt gatatcaccg tacagctgca gggcgatggt ccgatgaatc
    59041 tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc gtgcagggcg
    59101 aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca
    59161 ttaccccgag cgaaggcgaa cgctatcagg gcgtagttgg tctggaaggt gataccctgg
    59221 cggcctgcct agaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc
    59281 gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg
    59341 cgcaaaatac ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa
    59401 ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag
    59461 aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt
    59521 gcgccgatgc gctgaaaacg ctgcctgatg aagaagttta tagcatcctg gcggaagatg
    59581 gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata
    59641 ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc
    59701 cggcagagag ggctctgccg gattttacga cttaatggcc tgatgtgctg cgcttaccag
    59761 cgcctatgaa ataaatcgca aattattttg gtcagataca gcgtcgtatc cgataaaagc
    59821 gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga tcgcgccacg
    59881 ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt tctttcgtta
    59941 tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct gagtcaaagc
    60001 catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac
    60061 tattcagatc ttgctcgacc agtacgcagg cgtctttttc aatacaggtc atcacgttat
    60121 agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa
    60181 ttaacacatt ttcggctaat actttttccg atacctgttc ctgaagatgt tgctctccat
    60241 ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt
    60301 tcagcgactc aagcgccgca cgtccatagc gaccgctctg ttcgagcatg tagatagtta
    60361 cctggtctgg cgtgtttttg ccgctctgat aacgcgccat atgactgttc acgctaatac
    60421 gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc aataacgaaa
    60481 ccgcaaggtt cactttgccc tgaagaacaa ggggattttg cccattacgc cagacgatac
    60541 tgacaaaata ccccagcgac cacaccagta acaccagctc cgccagcgcg ccctgcaagc
    60601 ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cccccaggtg gcgacgaaca
    60661 catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc cccgtccagg
    60721 gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca
    60781 gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg
    60841 gcagggtgat gataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga
    60901 ttagcgtgaa caacttttga atagagtcga ttaaacgtga ctgtgtacgc gcgaggatca
    60961 ccgccagtgc ggtaaccaga ccaaatgtaa gatacataaa ccagtcggtt gcaaaaaaga
    61021 gcgtattgaa aaacgtgatg ccaacaagtt taaacaactc accccacagt aaaagcacca
    61081 gccacgtcag gccattggag agaaaaatca ccagtaatat aagtacgtta tgccatactg
    61141 actggtaaaa gtagctatag cggctactgc cattgcggat acgaaggctt tgttgtatcc
    61201 acggtagcaa caacattgcc atcaacagca gatagcagcc aaaatcccaa atcgcctttt
    61261 cagctctcca ggggttcatg ccatcagttt gccacttcag ccaaccgctc atccctgtcg
    61321 tggcaataaa caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa gagatcacgg
    61381 agaagagtaa aacggatgaa aacgcgacgg tcgcaggcac gccataaaca atccaactgt
    61441 gattttttcc ggcaagccag gcgataagca ggtagcaaac cagcccctga agtaatccgg
    61501 tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg ttggttatcc
    61561 agaatcaaaa ggtgggttaa ttatcgcatc cgggcagtag tattttgctt ttttcagaaa
    61621 ataatcaaaa aaagttagcg tggtgaatcg atactttacc ggttgaattt gcatcaattt
    61681 cattcaggaa tgcgattcca ctcacaatat tcccgccata taaaccaaga tttaaccttt
    61741 tgagaacatt ttccacacct aaaatgctat ttctgcgata atagcaaccg tttcgtgaca
    61801 ggaatcacgg agttttttgt caaatatgaa tttctccaga tacgtaaatc tatgagcctt
    61861 gtcgcggtta acacccccaa aaagacttta ctattcaggc aatacatatt ggctaaggag
    61921 cagtgaaatg cgcgttaaca atggtttgac cccgcaagaa ctcgaggctt atggtatcag
    61981 tgacgtacat gatatcgttt acaacccaag ctacgacctg ctgtatcagg aagagctcga
    62041 tccgagcctg acaggttatg agcgcggggt gttaactaat ctgggtgccg ttgccgtcga
    62101 taccgggatc ttcaccggtc gttcaccaaa agataagtat atcgtccgtg acgataccac
    62161 tcgcgatact ttctggtggg cagacaaagg caaaggtaag aacgacaaca aacctctctc
    62221 tccggaaacc tggcagcatc tgaaaggcct ggtgaccagg cagctttccg gcaaacgtct
    62281 gttcgttgtc gacgctttct gtggtgcgaa cccggatact cgtctttccg tccgtttcat
    62341 caccgaagtg gcctggcagg cgcattttgt caaaaacatg tttattcgcc cgagcgatga
    62401 agagctggca ggtttcaaac cagactttat cgttatgaac ggcgcgaagt gcactaaccc
    62461 gcagtggaaa gaacagggtc tgaactccga aaacttcgtg gcgtttaacc tgaccgagcg
    62521 catgcagctg attggcggca cctggtacgg cggcgaaatg aagaaaggga tgttctcgat
    62581 gatgaactac ctgctgccgc tgaaaggtat cgcttctatg cactgctccg ccaacgttgg
    62641 cgagaaaggc gatgttgcgg tgttcttcgg cctttccggc accggtaaaa ccaccctttc
    62701 caccgacccg aaacgccgcc tgattggcga tgacgaacac ggctgggacg atgacggcgt
    62761 gtttaatttc gaaggcggct gctacgcaaa aactatcaag ctgtcgaaag aagcggaacc
    62821 tgaaatctac aacgctatcc gtcgtgatgc gctgctggaa aacgtcaccg tgcgtgaaga
    62881 tggcactatc gactttgatg atggttcaaa aaccgagaac acccgcgttt cttatccaat
    62941 ctatcacatc gataacatcg ttaagccggt ttccaaagcg ggccacgcga ctaaggttat
    63001 cttcctgact gctgatgctt tcggcgtgtt gccgccggtt tctcgcctga ctgccgatca
    63061 aacccagtat cacttcctct ctggcttcac cgccaaactg gccggtactg agcgtggcat
    63121 caccgaaccg acgccaacct tctccgcttg cttcggcgcg gcgttcctgt cgctgcaccc
    63181 gactcagtac gcagaagtgc tggtgaaacg tatgcaggcg gcgggcgcgc aggcttatct
    63241 ggttaacact ggctggaacg gcactggcaa acgtatctcg attaaagata cccgcgccat
    63301 tatcgacgcc atcctcaacg gttcactgga taatgcagaa accttcactc tgccgatgtt
    63361 taacctggcg atcccaaccg aactgccggg cgtagacacg aagattctcg atccgcgtaa
    63421 cacctacgct tctccggaac agtggcagga aaaagccgaa accctggcga aactgtttat
    63481 cgacaacttc gataaataca ccgacacccc tgcgggtgcc gcgctggtag cggctggtcc
    63541 gaaactgtaa tgatttgaag ctggagaata tctatccagt atcttataga aagcaaaacg
    63601 ggaggcacct tcgcctcccg tttatttacc cttcttttgt cgtgccctgc gcccgcgtta
    63661 ccggcactgg cagccaggcg cgaatggaaa gcccgccccg ctcgctggtg ccaagctcca
    63721 gcatcccgtt atggttatcc acgatacgct gcacaattgc cagccctaat cccgtgccgc
    63781 taatggtgcg cgcactgtcg ccgcggacaa acggctggaa caggtgctta cgttgttccg
    63841 gcgcaattcc cggaccgtca tcttccacct ggaaccaggc gcgattcggc tccgttccgc
    63901 tgctgacttt gatccagcca ttgccataac gggcggcgtt gaccaccata ttcgccaccg
    63961 cgcgtttgat cgacagcggg tgcattttca cttcaatgct gccggggtaa agcgcggttt
    64021 caatttcccg ctcatagcca ctttcggcag caatcacctc accgagcact gcattaagat
    64081 ccgccatttc catcggcatc tcctgcccgg tgcgcaggta gtcgataaac tgctcaatga
    64141 tggcgttgca ctcttcgata tctttattga tcgattctgc cagatagcca tcctgctcgc
    64201 tcatcatctc agtcgccagg cgaatacgcg tcagcggcgt gcgcaagtcg tgacttaccc
    64261 ccgccatcag cagcgtgcgg tcatccgcca gttgcttaac accagccgcc atatggttaa
    64321 aggcacgggt aacggaacgc acctccgaag cgccatattc acgcagcggc ggcggaataa
    64381 tccctttacc aacctgcaag gctgcgtgtt cgagatcgac caacggtcgg ttctggatac
    64441 gaataaacag ccacgccccg cctatcgcca atagcataat cgccagcgta tagcggaaca
    64501 gcggagagaa atcgccctga tgaatttcgg tcagcggcac gcgtacccag atattgggcg
    64561 acagccaggt tttcagccag acgacaggcg aacttttgtt gacctcaacg cgcacttccg
    64621 tcgggccgcc cagttgctgt gccatctgat ggcttaagaa ttcatagtgt tgcgcccaac
    64681 gcagacctgc ctcttcggca gcctcgttgg agtagagaga gatccccagc tcacggtaga
    64741 tctcccgacg gaaagcggga ggcacaacca actgcgtgcc gtcctccagt tgcagtttat
    64801 cggtcatcaa catacgcact tcgtacgcga ggactttatt aaactgctgg aggctcggca
    64861 aaatcgcgaa gttcagcacc accagataag tcgtcaccag gctggcgaac agcaaggtga
    64921 cgatgagcaa taacgtacgg gcaaatgaac ttcgtggcga gaagcgcaat cgcctcatgc
    64981 tttagagccg tccggtacaa agacgtagcc caggccccaa acggtctgaa tgtaacgcgg
    65041 atgcgctgga tcttcttcta ccatgcggcg cagacgcgaa atctgcacgt cgatggagcg
    65101 ttccattgcg gaatattcac gaccacgggc aaggttcatc agcttatcgc gggagagcgg
    65161 ctcacgcgga tggctgacca gtgccttcag taccgcaaac tcaccgctgg tgagcggcat
    65221 cggctcatct tcgcggaaca tttcgcgcgt accgaggtta agtttgaact taccgaaagc
    65281 aattaccgcc tcttcctgtg acggtgcgcc tggcagttcg ttcgcctgac gacgcagcac
    65341 cgcacgaata cgggccagca gttcacgcgg gttaaacggt tttggaatgt agtcgtcagc
    65401 gccaatctcc aggcctacga tacggtccac ttcttcccct ttcgccgtca ccataatgat
    65461 cggcatcggg ttgctctgac tacgaagacg tcggcaaatc gacaagccat cttcaccagg
    65521 taacattaaa tccagtacca taagatggaa agattcacga gtcagcaggc gatccatctg
    65581 ttctgcatta gcgacgcttc gaacctggaa gccttgttcg gtgagataac gttccagcag
    65641 cgcacgcagg cgcatgtcgt catcgaccac cagaatcttg tagttctctt gcattgtttg
    65701 tactcccaaa ggttcgcaac aatttgtaag cgtgtattct taaaaaagct cacgttcgtc
    65761 accagctaaa tctggtatga atttcagcct aaattgttac aaagcatatt aaacagcagc
    65821 ttaagtatac aatttattcg gcgaaacatt attgattctg ttgatatgat cacgttatac
    65881 ccaatgtgcg cattatcaaa cagacaaagg gaatcaacga gatgaaaacg cccctggtta
    65941 cccgggaagg gtatgaaaaa ctcaaacaag agcttaatta tctctggcgt gaagaacgcc
    66001 cggaggtcac aaaaaaggtg acctgggccg caagtctggg cgaccgcagc gaaaatgctg
    66061 actatcagta taataaaaag cgtctgcgtg aaatcgaccg tcgcgtgcgc tatctcacta
    66121 aatgcctgga aaatctcaaa attgtcgatt actcccctca gcaggaaggc aaagtctttt
    66181 ttggcgcgtg ggtggagatt gaaaacgacg atggcgtgac tcaccgtttc cgtattgtcg
    66241 gctacgatga aatttttggc cgtaaagatt acatctctat cgattccccg atggcccgcg
    66301 cattgctgaa aaaagaagtc ggcgatctgg cggtggtgaa tacccctgcc ggggaagcga
    66361 actggtatgt taatgctatc gagtacgtga aaccgtaagg aagagtctta acctcctgcc
    66421 gatggctggc atttttgcca gccagtccgt ataactatcc cctgattttt gatccgaaaa
    66481 gatgaactca aaccatgatg aatgattcgt tctgccgcat tattgcgggt gaaattcagg
    66541 cgcgcccgga acaggttgac gctgccgttc gcctgcttga tgaagggaat accgtgccgt
    66601 ttatcgcacg ttatcgtaag gaaatcaccg gcggtctgga tgacacgcag ctgcgtaatc
    66661 tggaaacgcg tctgagctat ctgcgcgagc tggaagagag acgtcaggcg atcctcaaat
    66721 ccatttccga gcaaggcaaa ctcaccgatg atctggcgaa ggccatcaac gccaccctaa
    66781 gcaaaaccga actcgaagac ctctacctgc cctacaaacc taaacgccgc acccgcgggc
    66841 aaatcgccat tgaagcaggg cttgagccgt tggctgacct gctgtggagc gatccgtcac
    66901 acacgccaga agtcgccgct gcacaatatg ttgatgccga taaaggcgtg gcagatacca
    66961 aagccgcgct ggatggcgcg cgctatatcc tgatggaacg gtttgccgaa gatgccgcgc
    67021 tgctggcgaa agtgcgtgat tatctgtgga agaacgcgca tttggtttct acggtggtga
    67081 gcggtaaaga agaggaaggg gcgaaattcc gcgactattt cgatcatcac gaaccgttgt
    67141 ccacggtgcc ttctcaccgc gcgctggcga tgttccgtgg gcgtaacgaa ggcgtactcc
    67201 agctttcgct gaatgccgat ccgcagttcg atgagccgcc caaagagagc tattgcgagc
    67261 aaatcatcat ggatcacctt ggcctgcgcc tgaacaatgc cccggcggat agctggcgca
    67321 aaagcgtagt gagctggacc tggcgcatca aggtgctgat gcatctggaa accgaactga
    67381 tgggtaccgt gcgcgaacgc gcggaagatg aagcaatcaa cgtctttgcc cgtaacctgc
    67441 acgatctgct gatggcggcc cctgccggac tgcgtgcaac gatgggcctc gatccgggtc
    67501 tgcgtaccgg ggtaaaagtg gcggtggtcg atgccactgg caaactggtg gcgaccgata
    67561 ccatttaccc gcacaccgga caagccgcaa aagcagcgat gaccgttgct gccttgtgtg
    67621 aaaaacataa cgttgaactg gtggcgatcg gcaacggtac agcttcccgc gaaactgaac
    67681 gtttctatct cgacgtgcag aagcagttcc cgaaagtgac cgcacagaaa gtgatcgtca
    67741 gcgaagctgg cgcgtcggtt tactcagctt ccgagctggc agcgcaggag ttcccggatc
    67801 tcgacgtttc gctgcgtggt gcggtatcta tcgcccgccg tttgcaggat ccgctggcgg
    67861 agctggtgaa aatcgatccg aaatctatcg gcgtaggtca gtatcagcat gacgtcagcc
    67921 agacgcaact ggcccgcaaa ctggatgcag tagtagaaga ctgcgtaaac ggcgttggcg
    67981 tcgatctcaa caccgcttct gttccgctat taacccgcgt ggcgggcctg acgcgcatga
    68041 tggcgcaaaa catcgtggcc tggcgcgatg agaacggcca gttccagaac cgtcagcaac
    68101 tgctgaaagt cagccgtctg gggccgaaag ccttcgagca gtgcgcgggc ttcctgcgca
    68161 ttaaccacgg tgataacccg ctggacgcct ctaccgttca tccggaagcc tatccggtgg
    68221 tggaacgcat tctggcagca acacagcagg cactgaaaga tctgatgggt aacagcagcg
    68281 aactgcgtaa cctgaaagcg tctgacttta ctgatgaaaa attcggtgtg ccgacagtaa
    68341 ctgacatcat caaagagctg gaaaaaccgg gtcgcgatcc gcgtccggaa tttaaaaccg
    68401 ctcagtttgc cgatggcgtc gagacaatga acgacctgca accgggtatg atcctcgaag
    68461 gcgcagtgac caacgtcacc aactttggcg cgtttgtcga tattggcgtg catcaggacg
    68521 gcctggttca catctcttca ttgtcgaaca agtttgtgga agatccgcat accgtggtga
    68581 aagcgggcga cattgtgaag gtgaaagtgc tggaagtgga tcttcagcgt aaacgtatcg
    68641 ccctgactat gcgtctggac gagcagcctg gcgaaaccaa ctctcgtcgc ggcggcggta
    68701 atgaacgccc gcaaaacaac cgcccggcag ccaaaccacg cggtcgtgaa gcgcagcctg
    68761 ccggtaatag cgcgatgatg gatgcgctgg cggcagcgat gggtaaaaaa cgttaaacgc
    68821 ccggactggc ccctaaacct gcgcgataag gcgttcacgc cgcatctggc aacgaacgct
    68881 ttgcctgaca taaaagtgcc ggagaatatc tccggcactt ttattccaca gccaaactca
    68941 caatatactc cgttaatatt tatcacttca ttaacaactg aaaccttaat taaacattag
    69001 ccagtccggg taactcacta ttcgaattat attttcgctg cgatataacc ttgagccaca
    69061 tcaacattga gtcagattat tattcaaacc aacattcgta cacattttaa gtattgctga
    69121 tagaaaccat tctcattatc attgtgttgt tgattattta atctctcctt tgttggcaaa
    69181 tcatctggtc tcatgtcgcg gtcaaacgcc ccatgaggta gttatccagt taatgagaaa
    69241 caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg gcttttcccg
    69301 tgaaatcagc ccggcatatc gccaaaaact gctttctcta ggcatgttac ctggctcctc
    69361 ttttgatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa cccgtcgtgt
    69421 gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg tttcctgtta
    69481 atacggtgat aacaacaatg aaaaaattaa ccattggctt aattggtaat ccaaattctg
    69541 gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt aactgggctg
    69601 gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag gtcacgctgg
    69661 tggacctgcc cggcacctat tctctgacca ccatctcatc gcagacctcg ctcgatgagc
    69721 aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac gtggtggatg
    69781 cgtctaacct tgagcgtaac ctgtacctga cgctgcaact gctggaactc ggcattccct
    69841 gcatcgtggc gctgaacatg ctcgacattg ccgagaagca aaatattcgt attgaaattg
    69901 atgctctatc ggcacgtctt ggctgcccgg tgatcccgct ggtttcaacc cgtggtcgcg
    69961 gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat gtggaactgg
    70021 tgcattacgc acagccgcta ctcaacgaag cagattcact ggcaaaagtg atgccttccg
    70081 acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc gatatctaca
    70141 gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc caccctcgcc cgtctgcgta
    70201 atgaaatgga tgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc attgctgcca
    70261 tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc actgcggtag
    70321 ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg atgtacctga
    70381 tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac gtcggctccg
    70441 tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc ccggactggc
    70501 tgactatctt cctcgcccag ggcctgggtg gcggtattaa caccgtgctg ccactggtgc
    70561 cgcagatcgg catgatgtac ctgttcctct ccttccttga ggactccggc tatatggcgc
    70621 gtgcagcgtt tgtgatggac cgcctgatgc aggcgctggg cttgccgggg aaatcctttg
    70681 tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggcgca cgtacgcttg
    70741 atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc tgcggcgcgc
    70801 gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt gccctggcgg
    70861 ttttctcgct gtatatgctc ggtatcgtga tggcggtgct gactggcctg atgctcaagt
    70921 acaccatcat gcgcggtgaa gcaacgccgt ttgtcatgga gctgccggtc taccatgtac
    70981 cacacgttaa aagcctgatt atccagacct ggcagcgtct gaagggcttc gttctgcgtg
    71041 ctggtaaagt gattatcatc gtcagcattt tcctgagcgc tttcaacagc ttctcgttga
    71101 gcgggaaaat cgtcgataac atcaacgact cggcgctggc ttccgtcagc cgggtgataa
    71161 cccctgtctt caagccgatt ggcgtacatg aagataactg gcaggcaacg gttggcctgt
    71221 ttacgggcgc aatggcaaaa gaagtggtgg tgggtacgct caacaccctc tacaccgcag
    71281 aaaatattca ggacgaagag ttcaatccgg cggaattcaa cctcggtgaa gagctgttca
    71341 gtgcggtaga tgaaacctgg cagagcctga aagacacctt cagccttagc gtactgatga
    71401 accccattga agctagcaaa ggcgacggtg aaatgggtac cggggcgatg ggcgtgatgg
    71461 atcagaaatt cggtagcgca gctgccgcat acagctacct gattttcgtc ctgctgtatg
    71521 taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc tggatgggct
    71581 tctccatcct gtgggggctg aatatcgctt actcactggc aacattgttc tatcaggtcg
    71641 ccagctacag tcagcaccca acttacagcc tggtgtgcat tctgggggta atcctgttta
    71701 acatcgtagt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc gaactgctgg
    71761 caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat tgccattaat
    71821 ggcttcactt attcaggtac gtgatttgct ggcgttacgg tgccgtatgg aagcgaccca
    71881 gataagccag acattgaaca ctccgcagcc aatgattaac gccatgctgc aacagctgga
    71941 aagcatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct ctggcagttg
    72001 taaaagctgc ccggaaggca aagcctgtct gcgcgagtgg tgggcgctgc gttaaccata
    72061 ctccatcgcc tgttttgaaa agcccggtat gcgtttgcat ccgggctttt ttgcgtgcgg
    72121 ctttccataa aaatgcaatt cttgcagcgc aacgtaagtt cctctgaaag cgtctcgcag
    72181 ggatgaaaac tcgctaatgc acagatgtgg agtggcgcgt agagtcgcgg cattcaaaca
    72241 acaggtgaag gaacgccatg agtaaaaagc agagttccac cccacatgat gcgctgttca
    72301 aactcttttt acgccaaccg gagacggcac gcgattttct tgcgtttcat ttaccggcac
    72361 ccattcacgc gctctgtgat atgaaaaccc tcaagctgga gtcgagcagc tttattgatg
    72421 acgatctgcg tgaaagctat tccgatgtgc tgcggtcggt gaaaacggaa caaggaccag
    72481 gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc gcatttcgca
    72541 tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac aaaacgttgc
    72601 cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc tattcgctgt
    72661 gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc tccgcatttc
    72721 cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga cgtatggcgc
    72781 tgctggagtt aattcaaaaa catattcgtc aacgcgatct gatggggctg gtagagcaaa
    72841 tggcctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg ctgtttaatt
    72901 acatactgca aactggcgac gctgtacgtt ttaacgattt tatcgacggc gttgccgaac
    72961 gttcaccgaa acacaaggag agtttaatga ctattgcgga aagattgcgg caggaggggg
    73021 aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc cctcttgcag
    73081 acatcatgcg atttaccggg ctgtcagaag aagagttggc tgcggcgagt cagtagatac
    73141 tctaccctgc ttaagtgcta ctttgtgcta tcttgcatgt aacacaaagt agcacttagg
    73201 gaactgatat gcagacagtg aaattacggc aacaaggagg agccatgatc gttactatcc
    73261 cacgagatct tgccatcgat cttggctggt caccgggcac agagcttacc gtggagaaaa
    73321 aaggcgatag cgttaatttg cgcgccacag agcacaaacc gcgtgggcgt cttaccgtcg
    73381 cgcagttgtt gagccaaatc gatgaaagcg aaattacgga gctaaatcag tccacagaag
    73441 gatgggctga agggaaaaaa ggtaatgaag catggtaaag gcacggacgc cacatcgtgg
    73501 tgagatctgg tattttaacc ctgatccggt tgccgggcat gaacttcagg ggccacatta
    73561 ttgcattgtg gtaacggaca aaaaactcaa caatgtttta aaagttgcta tgtgctgccc
    73621 gatttcaaca ggggcaaatg cagcacgttc cacaggggtg acggtgaacg tcctcccctg
    73681 tgatacgcaa accggtaacc tgcatggcgt tgtactttgt caccagctaa aagccgtcga
    73741 tcttattgcc cgtggcgcta aatttcatac cgttgccgat gaaaaattga ttagtgaagt
    73801 tatcagtaaa ctggtgaatt taatcgaccc acaataatta gctctcactt accaccaaca
    73861 ccctctgctt caacgccacc agcaggtgac aaaactcgac cggatgcgaa ataaacggcg
    73921 catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta tccagcatcg
    73981 gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa aacggcatgg
    74041 acacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc ccgccattaa
    74101 gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc cgcgcatcct
    74161 ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa ccgctccact gtacgctgaa
    74221 aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc cccggccact
    74281 cgtcacgagc actaaaataa ggtgacgacg ccacggtgac cagcgcctga acaggctcgg
    74341 gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct aaccaaatgg
    74401 ctttatcagg tgcctgttgc agcacggctt cggccatatc agcaagtgac agcgcaccaa
    74461 atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa tgcgagctaa
    74521 gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc agcagcacaa
    74581 gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc tattgttctc
    74641 ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg ctatgccgaa
    74701 tgccactggc gttaggtcat tgggggattt gttcggtctg ttcacgcgcc acccgcacag
    74761 ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcccatctt ccctgcggtc
    74821 gctgcctgca aaaaccgccg ccctggcaaa gactggtcac ggttgccgac tatgcgccgc
    74881 cgttaagtcc gcttatccac cagcttaaat tttcccggcg cagtgaaatc gccagcgccc
    74941 tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggg ttgcaattgc
    75001 cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg ggatttaatc
    75061 agagcgattt gctgtgtcag ccgttatcac gctggttgca ctgccaatgg gatagcgaag
    75121 ccgtcacacg tacacgggcc actgcgaccc agcattttct cagtgcccgg ctgcgcaagc
    75181 gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca aggtcgccat atggtgattg
    75241 tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg cttttacgca
    75301 atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg
    75361 ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat ccgtatttcc
    75421 gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg gacacaaatc
    75481 cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggtgtttc ttattgtccg
    75541 ccggatgccg tggaagccac cgacactgcc ctgaaatttg acctgctgac cgcgtatgtt
    75601 gatgagttaa gcgcaccata cctggaagat gcagagatcg actttgttac cgaccagttg
    75661 ggttctcagt taacgctgaa agccccgaac gccaaaatgc gtaaagtggc agacgatgca
    75721 ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca gcttgctggt
    75781 cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat tctgcaattt
    75841 ggcggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg gatcgagaag
    75901 cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga acaccagcgc
    75961 ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat gcgacgctaa
    76021 agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcacagcat tgctttggca
    76081 aatccctccc ttcgtttccc cgcgataata tgaccaacct ctcataattt aaatttaccc
    76141 cgctctggtg attctcaaac gccagatgtt acctgtatca ttcacatggg taccaaacat
    76201 actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg tgccgcaata
    76261 tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt tacccataac
    76321 aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt aatcttgttg
    76381 ttgctcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct cgtggcgctt
    76441 gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat caaagccggt
    76501 gtcggcggga cgctcggtag ccttgccctg atcatgggtt ttggcgcaat gctggcaaac
    76561 tgcggtggcg cacaacgtat cgccaccacg ctgattgcca aatttggtaa aaagcacatc
    76621 cagtgggcgg tggtgttaac cggttttacc gttggttttg ccctgttcta tgaagtgggc
    76681 ttcgtgctga tgctgccgct ggtgtttacc atcgcggctt ccgcgaatat tccattgctg
    76741 tatgttggtg taccaatggc ggctgcactg tctgtgaccc acggcttcct gccaccgcat
    76801 ccgggcccga ctgcgattgc caccattttc aatgccgata tgggtaaaac cctgctgtac
    76861 ggtactattc tggcaatccc gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg
    76921 aaaggtatcg ataagccaat cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa
    76981 gagatgccga gctttggcgt cagcgtctgg acctctctgg tgccggttgt gctgatggcg
    77041 atgcgtgcga ttgccgaaat gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag
    77101 ttcctcggtg acccggtaat ggcaacgctg attgccgtgc tgattgcgat gttcaccttt
    77161 ggtctgaacc gtggtcgttc aatggatcag attaacgaca cgctggtttc ttccatcaaa
    77221 atcattgcga tgatgctgtt gatcatcggt ggtggcggtg cgttcaagca ggtgctggta
    77281 gacagcggcg tggacaaata cattgcttcc atgatgcacg aaaccaacat ttctccgctg
    77341 ctgatggcct ggtcgattgc tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg
    77401 gcaatcactg cgggtggtat cgcggcaccg ctgattgcaa cgacaggtgt tagcccagag
    77461 ctgatggtta ttgcggtggg ttccggtagt gtgattttct ctcatgtgaa ctatccgggc
    77521 ttctggctgt tcaaagagta ctttaacctg actatcggcg agaccatcaa atcctggtcg
    77581 atgctggaaa ccattatctc ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg
    77641 atttgataac actgactgcc ggatgcatcg catccgacat caaccgcact ctacttcttc
    77701 ttcgctgcag ctctgcgccg tctgtccaaa tccttcagca acttgttcac gccatcatcg
    77761 gcaaacatcg actcaagcgt tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg
    77821 gtgctaggaa tattcaccgg ttcggccata tccagccagt cttccggctg tagtcctaac
    77881 agagcactgt tactgtcggc aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc
    77941 gacatcaacg atgccttatg cccggcacgt tttggcagac aaccatattt atgcagtgca
    78001 tccagcagcc cttgcttcgc cagttcgcga tcctgataca gaccgcgcag taccacttca
    78061 tccggataca gccccagggt tttgcccagc gttagatccc cgctctccca gtaaccacgc
    78121 agcgttggca ggtcatgtgt cgccgcaacc gccatcgact gctccggata cgctttcggt
    78181 gcacggaacg tcttctcgtg gtcgttttcg aaatagagca ctttgtaaga gtacacaccg
    78241 ctgctgcgca gcttaccgac aatctctacc ggtacggtac cgagatcttc accaatcacc
    78301 atacagcgat gacgtttact ttcgagtgcc agaatcgaga gcagatcatc caccggatag
    78361 tgaacatacg cgccctgatc tgccgtctcg ccatacggta tccaccacaa acgcagcatc
    78421 gacatcacat ggtcaattcg taatgcgccg cagttttgca tattggcacg caacagctcg
    78481 ataaacggtt cataggcacg cgcggtgatg atatgcgggt ccattggcgg taatccccag
    78541 ttctgcccca acgggccgag gatatccggc ggcgcgccaa ccgatgcttt caggcaatat
    78601 agttcacggt cacaccaggt ttccgcccca ccttccgcta cgccaaccgc cagatcacga
    78661 tacaagccaa tcggcatttc atagccctgg cttatctccc agcaggcggc aaactggctg
    78721 taagccagcc actgcaacca gagataaaaa tcgacgtcat cacgatgttc ttcgcagaac
    78781 tgacgcactt ctggtgaatc cacgttctta tacatctctg gccatgcagg ccagccccag
    78841 cgcatttcgt cctctttcac ttgctgggca tgtagcgcat caaaggctgc ctgccagaac
    78901 aggctgtcgc cctgctctgc aacaaactgg cgaaacgcgg ccatctgctc atcatcacgt
    78961 tgcgcgaaac ctttccacgc cattcgtaat gctgtcattt ttagggcggt aaccgtggag
    79021 taatcgaccc agtcggcatc gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc
    79081 caccaggcct gagcctcttc gctaagatgg aaatcttcaa cggcgttaac gtcgatataa
    79141 atcacattca gccaacggcg agaagacggg ctgtatgggc tggcgctctc cggatttgcc
    79201 ggatagagcg catgaatcgg gttcaggcca atgaacgacc cgccacgttt tgccacatcc
    79261 accagcatcg ctttgagatc gccaaaatcc ccaatacccc agtttttttc cgatcgcagc
    79321 gtataaagct gaacgcaggc accccacagc ttttgtttat tcagcaaagc ctgcggttcg
    79381 taacaacgtt tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt
    79441 gttagcgtgt gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtt
    79501 acatggcctt tgtactgcgt tccttcttcg gtggtcagct gccagctata ttcgccgctg
    79561 ccctccacca ccatcggcat ttttttgccg ctggtataaa ccatgacatt cgggactggc
    79621 gttaccgcca ctttcgtggc ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt
    79681 tcggcgctaa tcgactgcgg tttaccgtgg gcattgatgt aattggggct aatccccgcc
    79741 gccagcgcgg cattatccag acgtttgctt tccatcgagc ttccttagcg ttttgcctgc
    79801 cagatacgag cctgataatc gcgaatagag cgatccgagc taaacatacc gcagcgggcg
    79861 gtattgagga tcgccgcgcg agtccaggcc tcctggtcgc ggtacagcac atccacctgc
    79921 ttttgtgcct caacataggc tgcgaaatcc gccatcacca gatacggatc gccgccctgt
    79981 ttgccgatac tgtgcagcat ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg
    80041 ctttccagct ctttcaatac tgcgtccagc accttatctt tcttccgcca tttcaccggg
    80101 tcgtagcctt tggccagaat agccttcact tgttccacgg tatgaccaaa aataaagata
    80161 ttttcttcac cgactttctc ggcgatttca acgttcgcac catccagcgt accgacagta
    80221 agcgcaccat tgagcgccag tttcatattg ccggtaccgg aagcttcttt acctgcagtc
    80281 gaaatttgtt cggagatatc cgccgccggg atcagttttt ccgccgccga aacgcaataa
    80341 tccggcagga acaccacctt caacttatcg ccaaccagtg gatcgttgtt gatcacgtca
    80401 gccactttgt tgatcgcaaa gataatattc ttcgccaggt agtagcccgg tgccgctttc
    80461 gcgccgaaga ggaagacgcg cggtacgcga tcagcctgca ggttttcacg aatttctttg
    80521 tacaacgcca gaatatgcag cagattcagg tgctggcgtt tgtactcatg cagacgtttg
    80581 atctgaatat cgaaaatcgc ctgtggattg atctcaatac cggtacgaac tttcacaaac
    80641 tccgccagac ggactttatt cgcctgcttg atctcgcgat attgctgacg gaatttcgca
    80701 tcatcagcga atttttccag attgatcagc tgatcgagat cgttagccca ctctttttgc
    80761 agtgatttat ccaacagagc cgccagtgca gggttgcact gtttgatcca gcgacgtggg
    80821 gtaataccgt tggtgacgtt atggaatttg ttcggccata gctggtgata ttccgggaac
    80881 agatctttca ccaccagatc cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca
    80941 accacacaca ggttcgccat atgcacttgt ttatcgtgca ccaccgccag tttggcccac
    81001 actttttcat cgcccggcca ggttttctcc accagcgttt taaagcgagt attaatttcg
    81061 ttaataatct gcatatggcg cggcagtaag cctttcacca gtttcacatc ccagcgttcc
    81121 agcgcttctg gcatcagggt atggttggtg taagcgaaag ttttgctggt gatggcccag
    81181 gcgtcatccc agctcatctg gtgctcatcg gtcagcacgc gcagcagttc tggaatcgcg
    81241 atagttggat gggtatcgtt cagctgaata acttcgtaat ccgccagttc gtgcagttta
    81301 cgccccgcca gatgatggcg acgcaaaata tccgctaccg aacaggcaca ctggaagtat
    81361 tgctgcatca ggcgcagctt tttaccggca gtatggttgt cgtttggata gagaactttg
    81421 gtcagttttt ccgcattgat gccttgctgt tcggcgcgca ggaaatcacc gtcgttaaat
    81481 ttagttagat caaacggatg cgcatgcgtc gcctgccaca gacgcagcgg ctgcgccacg
    81541 ccattacgat agccgacaac ggggagatcc cacgcttgac cggtaatggt aaactccggc
    81601 tcccagcgtc cgtctttcat cactttaccg ccaatcccta cctgcacatc cagtgcttcg
    81661 ttgtggcgga accacgggta gttactgcga tgccagtcat ccggcgcttc aacctgtttg
    81721 ccatcgacaa aagactggcg gaacaaacca tattgatagt tcagaccgta acccgtcgca
    81781 gactgaccga cagttgccat tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg
    81841 ttacccagcg ccgggtcgat ctcttcttcc agcaggtcag tcaggttgat gtcataagcc
    81901 ttcaacgaat cctgtacatc ctgataccag ccgagattca acaggttgtt gcccgtcaga
    81961 cgaccaatca aaaactccat tgagatgtag ttaacatgtc gctgattcgc caccggtttg
    82021 gcgaatggct gagcacgcag catttcggcc agtgcttcac tcactgccag ccaccactgg
    82081 cgaggagtca tttcagccgc agaatttaag ccataacgct gccactgacg tgaaagcgct
    82141 tcctgaaatt gcttatcgtt aaaaataggt tgtgacatag gagttccact tttcttagat
    82201 tttcaacaca acgttatcgc tagtttgcca ggctcgatgt tgaccttcct catcctgcgg
    82261 gggattaggc agggaggagt tgcggggatg agcaaggaaa tgtgatctcg accacttaaa
    82321 gctagtgcaa accacaggat tagcagcaaa tcaatgccat acagcgcaga aaatctgtat
    82381 ctaagtgcaa aaaatggccg ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta
    82441 agtaaaggag tgaaacagtt tcacaagtaa aatatccagt gtgctccatc tcattcttaa
    82501 tagatttatt aagatcatct ttttagatgg cactttcatc aggaatgaag aagaaaccct
    82561 tgcttaaatg aatctgat
//
LOCUS       DADMFL010000003        76417 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.003, whole genome shotgun sequence.
ACCESSION   DADMFL010000003 DADMFL010000000
VERSION     DADMFL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.003"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..546
                     /locus_tag="JNZ27_000177"
     CDS             <1..546
                     /locus_tag="JNZ27_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAY8662659.1"
                     /translation="LFPINTGAHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKE
                     NSLKRVINSFNSELMEEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEV
                     IEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINAS
                     YTQLEIKHSQPKNRASGKRVS"
     gene            1089..1581
                     /locus_tag="JNZ27_000178"
                     /pseudo
     CDS             1089..1581
                     /locus_tag="JNZ27_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1684..2187
                     /locus_tag="JNZ27_000179"
     CDS             1684..2187
                     /locus_tag="JNZ27_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY8662660.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2254..3711)
                     /gene="nuoN"
                     /locus_tag="JNZ27_000180"
     CDS             complement(2254..3711)
                     /gene="nuoN"
                     /locus_tag="JNZ27_000180"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY8662661.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGGSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3718..5247)
                     /gene="nuoM"
                     /locus_tag="JNZ27_000181"
     CDS             complement(3718..5247)
                     /gene="nuoM"
                     /locus_tag="JNZ27_000181"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY8662662.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5411..7252)
                     /gene="nuoL"
                     /locus_tag="JNZ27_000182"
     CDS             complement(5411..7252)
                     /gene="nuoL"
                     /locus_tag="JNZ27_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY8662663.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7249..7551)
                     /gene="nuoK"
                     /locus_tag="JNZ27_000183"
     CDS             complement(7249..7551)
                     /gene="nuoK"
                     /locus_tag="JNZ27_000183"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY8662664.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7548..8102)
                     /gene="nuoJ"
                     /locus_tag="JNZ27_000184"
     CDS             complement(7548..8102)
                     /gene="nuoJ"
                     /locus_tag="JNZ27_000184"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY8662665.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8114..8656)
                     /gene="nuoI"
                     /locus_tag="JNZ27_000185"
     CDS             complement(8114..8656)
                     /gene="nuoI"
                     /locus_tag="JNZ27_000185"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY8662666.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8671..9648)
                     /gene="nuoH"
                     /locus_tag="JNZ27_000186"
     CDS             complement(8671..9648)
                     /gene="nuoH"
                     /locus_tag="JNZ27_000186"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY8662667.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9645..12371)
                     /gene="nuoG"
                     /locus_tag="JNZ27_000187"
     CDS             complement(9645..12371)
                     /gene="nuoG"
                     /locus_tag="JNZ27_000187"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY8662668.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12424..13761)
                     /gene="nuoF"
                     /locus_tag="JNZ27_000188"
     CDS             complement(12424..13761)
                     /gene="nuoF"
                     /locus_tag="JNZ27_000188"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY8662669.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13758..14258)
                     /gene="nuoE"
                     /locus_tag="JNZ27_000189"
     CDS             complement(13758..14258)
                     /gene="nuoE"
                     /locus_tag="JNZ27_000189"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY8662670.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14261..16063)
                     /gene="nuoC"
                     /locus_tag="JNZ27_000190"
     CDS             complement(14261..16063)
                     /gene="nuoC"
                     /locus_tag="JNZ27_000190"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY8662671.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16157..16819)
                     /locus_tag="JNZ27_000191"
     CDS             complement(16157..16819)
                     /locus_tag="JNZ27_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY8662672.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16835..17278)
                     /gene="nuoA"
                     /locus_tag="JNZ27_000192"
     CDS             complement(16835..17278)
                     /gene="nuoA"
                     /locus_tag="JNZ27_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY8662673.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17910..18848)
                     /gene="lrhA"
                     /locus_tag="JNZ27_000193"
     CDS             complement(17910..18848)
                     /gene="lrhA"
                     /locus_tag="JNZ27_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY8662674.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19773..20990
                     /locus_tag="JNZ27_000194"
     CDS             19773..20990
                     /locus_tag="JNZ27_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY8662675.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21074..21673
                     /gene="yfbR"
                     /locus_tag="JNZ27_000195"
     CDS             21074..21673
                     /gene="yfbR"
                     /locus_tag="JNZ27_000195"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY8662676.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21732..23564)
                     /locus_tag="JNZ27_000196"
     CDS             complement(21732..23564)
                     /locus_tag="JNZ27_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY8662677.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23651..24301)
                     /gene="hxpA"
                     /locus_tag="JNZ27_000197"
     CDS             complement(23651..24301)
                     /gene="hxpA"
                     /locus_tag="JNZ27_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY8662678.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24312..24806)
                     /locus_tag="JNZ27_000198"
     CDS             complement(24312..24806)
                     /locus_tag="JNZ27_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY8662679.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24889..25344)
                     /locus_tag="JNZ27_000199"
     CDS             complement(24889..25344)
                     /locus_tag="JNZ27_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY8662680.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25682..26884
                     /gene="ackA"
                     /locus_tag="JNZ27_000200"
     CDS             25682..26884
                     /gene="ackA"
                     /locus_tag="JNZ27_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY8662681.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26959..29103
                     /gene="pta"
                     /locus_tag="JNZ27_000201"
     CDS             26959..29103
                     /gene="pta"
                     /locus_tag="JNZ27_000201"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY8662682.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29293..30813
                     /gene="yfcC"
                     /locus_tag="JNZ27_000202"
     CDS             29293..30813
                     /gene="yfcC"
                     /locus_tag="JNZ27_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY8662683.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQLFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30846..31388)
                     /gene="yfcD"
                     /locus_tag="JNZ27_000203"
     CDS             complement(30846..31388)
                     /gene="yfcD"
                     /locus_tag="JNZ27_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY8662684.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31446..31997)
                     /gene="yfcE"
                     /locus_tag="JNZ27_000204"
     CDS             complement(31446..31997)
                     /gene="yfcE"
                     /locus_tag="JNZ27_000204"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY8662685.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32053..32697)
                     /gene="yfcF"
                     /locus_tag="JNZ27_000205"
     CDS             complement(32053..32697)
                     /gene="yfcF"
                     /locus_tag="JNZ27_000205"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY8662686.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32833..33480
                     /gene="yfcG"
                     /locus_tag="JNZ27_000206"
     CDS             32833..33480
                     /gene="yfcG"
                     /locus_tag="JNZ27_000206"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY8662687.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33537..33899
                     /gene="folX"
                     /locus_tag="JNZ27_000207"
     CDS             33537..33899
                     /gene="folX"
                     /locus_tag="JNZ27_000207"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY8662688.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIIINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33920..34813
                     /locus_tag="JNZ27_000208"
     CDS             33920..34813
                     /locus_tag="JNZ27_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY8662689.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34861..35751)
                     /locus_tag="JNZ27_000209"
     CDS             complement(34861..35751)
                     /locus_tag="JNZ27_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8662690.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35948..36721)
                     /gene="hisP"
                     /locus_tag="JNZ27_000210"
     CDS             complement(35948..36721)
                     /gene="hisP"
                     /locus_tag="JNZ27_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY8662691.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLEKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36729..37445)
                     /locus_tag="JNZ27_000211"
     CDS             complement(36729..37445)
                     /locus_tag="JNZ27_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8662692.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37442..38128)
                     /locus_tag="JNZ27_000212"
     CDS             complement(37442..38128)
                     /locus_tag="JNZ27_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8662693.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38218..39000)
                     /gene="hisJ"
                     /locus_tag="JNZ27_000213"
     CDS             complement(38218..39000)
                     /gene="hisJ"
                     /locus_tag="JNZ27_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY8662694.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39221..40003)
                     /gene="argT"
                     /locus_tag="JNZ27_000214"
     CDS             complement(39221..40003)
                     /gene="argT"
                     /locus_tag="JNZ27_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY8662695.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40269..40838)
                     /locus_tag="JNZ27_000215"
     CDS             complement(40269..40838)
                     /locus_tag="JNZ27_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8662696.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40933..42450)
                     /gene="purF"
                     /locus_tag="JNZ27_000216"
     CDS             complement(40933..42450)
                     /gene="purF"
                     /locus_tag="JNZ27_000216"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY8662697.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42487..42975)
                     /gene="cvpA"
                     /locus_tag="JNZ27_000217"
     CDS             complement(42487..42975)
                     /gene="cvpA"
                     /locus_tag="JNZ27_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY8662698.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAISVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43234..43896)
                     /gene="dedD"
                     /locus_tag="JNZ27_000218"
     CDS             complement(43234..43896)
                     /gene="dedD"
                     /locus_tag="JNZ27_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY8662699.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43886..45154)
                     /gene="folC"
                     /locus_tag="JNZ27_000219"
     CDS             complement(43886..45154)
                     /gene="folC"
                     /locus_tag="JNZ27_000219"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY8662700.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45224..46138)
                     /gene="accD"
                     /locus_tag="JNZ27_000220"
     CDS             complement(45224..46138)
                     /gene="accD"
                     /locus_tag="JNZ27_000220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY8662701.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46294..46953)
                     /locus_tag="JNZ27_000221"
     CDS             complement(46294..46953)
                     /locus_tag="JNZ27_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8662702.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTTGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47036..47848)
                     /gene="truA"
                     /locus_tag="JNZ27_000222"
     CDS             complement(47036..47848)
                     /gene="truA"
                     /locus_tag="JNZ27_000222"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY8662703.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47848..48861)
                     /locus_tag="JNZ27_000223"
     CDS             complement(47848..48861)
                     /locus_tag="JNZ27_000223"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8662704.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48927..50063)
                     /gene="pdxB"
                     /locus_tag="JNZ27_000224"
     CDS             complement(48927..50063)
                     /gene="pdxB"
                     /locus_tag="JNZ27_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY8662705.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50162..51157
                     /locus_tag="JNZ27_000225"
     CDS             50162..51157
                     /locus_tag="JNZ27_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY8662706.1"
                     /translation="MIQPISAPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(51154..52332)
                     /locus_tag="JNZ27_000226"
     CDS             complement(51154..52332)
                     /locus_tag="JNZ27_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8662707.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGKRPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52607..53827)
                     /gene="fabB"
                     /locus_tag="JNZ27_000227"
     CDS             complement(52607..53827)
                     /gene="fabB"
                     /locus_tag="JNZ27_000227"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY8662708.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53986..55992
                     /gene="mnmC"
                     /locus_tag="JNZ27_000228"
     CDS             53986..55992
                     /gene="mnmC"
                     /locus_tag="JNZ27_000228"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY8662709.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDAYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56113..56391)
                     /locus_tag="JNZ27_000229"
     CDS             complement(56113..56391)
                     /locus_tag="JNZ27_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY8662710.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56425..56973)
                     /locus_tag="JNZ27_000230"
     CDS             complement(56425..56973)
                     /locus_tag="JNZ27_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY8662711.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56973..57782)
                     /locus_tag="JNZ27_000231"
     CDS             complement(56973..57782)
                     /locus_tag="JNZ27_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY8662712.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57782..58606)
                     /gene="mepA"
                     /locus_tag="JNZ27_000232"
     CDS             complement(57782..58606)
                     /gene="mepA"
                     /locus_tag="JNZ27_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY8662713.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58610..59695)
                     /gene="aroC"
                     /locus_tag="JNZ27_000233"
     CDS             complement(58610..59695)
                     /gene="aroC"
                     /locus_tag="JNZ27_000233"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY8662714.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59730..60662)
                     /gene="prmB"
                     /locus_tag="JNZ27_000234"
     CDS             complement(59730..60662)
                     /gene="prmB"
                     /locus_tag="JNZ27_000234"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8662715.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMHTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60828..61379
                     /gene="smrB"
                     /locus_tag="JNZ27_000235"
     CDS             60828..61379
                     /gene="smrB"
                     /locus_tag="JNZ27_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY8662716.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61483..62373)
                     /locus_tag="JNZ27_000236"
     CDS             complement(61483..62373)
                     /locus_tag="JNZ27_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY8662717.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQFTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62360..62884)
                     /locus_tag="JNZ27_000237"
     CDS             complement(62360..62884)
                     /locus_tag="JNZ27_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8662718.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62881..63351)
                     /locus_tag="JNZ27_000238"
     CDS             complement(62881..63351)
                     /locus_tag="JNZ27_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8662719.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63348..63896)
                     /locus_tag="JNZ27_000239"
     CDS             complement(63348..63896)
                     /locus_tag="JNZ27_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8662720.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63901..64623)
                     /locus_tag="JNZ27_000240"
                     /pseudo
     CDS             complement(63901..64623)
                     /locus_tag="JNZ27_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64643..67284)
                     /gene="yfcU"
                     /locus_tag="JNZ27_000241"
                     /pseudo
     CDS             complement(64643..67284)
                     /gene="yfcU"
                     /locus_tag="JNZ27_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67366..67929)
                     /locus_tag="JNZ27_000242"
     CDS             complement(67366..67929)
                     /locus_tag="JNZ27_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8662721.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68613..69098)
                     /gene="sixA"
                     /locus_tag="JNZ27_000243"
     CDS             complement(68613..69098)
                     /gene="sixA"
                     /locus_tag="JNZ27_000243"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY8662722.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69301..71445)
                     /gene="fadJ"
                     /locus_tag="JNZ27_000244"
     CDS             complement(69301..71445)
                     /gene="fadJ"
                     /locus_tag="JNZ27_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY8662723.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEINALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71445..72755)
                     /gene="fadI"
                     /locus_tag="JNZ27_000245"
     CDS             complement(71445..72755)
                     /gene="fadI"
                     /locus_tag="JNZ27_000245"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY8662724.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72935..73219)
                     /locus_tag="JNZ27_000246"
     CDS             complement(72935..73219)
                     /locus_tag="JNZ27_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY8662725.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73591..74931
                     /gene="fadL"
                     /locus_tag="JNZ27_000247"
     CDS             73591..74931
                     /gene="fadL"
                     /locus_tag="JNZ27_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY8662726.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75297..76355
                     /locus_tag="JNZ27_000248"
     CDS             75297..76355
                     /locus_tag="JNZ27_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662727.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        19330 a        20454 c        18730 g        17903 t
ORIGIN      
        1 ttattcccca taaatactgg cgcacactgg atattagttt cgctacagaa aatagtaaat
       61 aaaaaaaata ataaactaca aataaaatgc gtcatattca actcattgcg tgcactaggc
      121 catgataaag aaaattcact taagcgtgtc attaatagtt ttaattctga actcatggaa
      181 gaaatgtcga ataataatat aaaagttcat ttaaatgaac cagagataat atttttacat
      241 gccgatcttc agcaatactt aagccaaagt tgcggtgcgt ttgtgtgcat ggcagcccag
      301 gaagtgattg aacaaaggga aagcaattct gacagcgccc cctatacgtt attaaaaaac
      361 tatgctgaca gatttaaaaa atattcagca gaagagcagt acgaaattga ttttcaacat
      421 cgactggtaa acagaaattg ttatttagat aaatatggcg atgcacgaat caatgcttct
      481 tatacacaat tagaaataaa acactcacaa cccaaaaata gagcatccgg caaaagagtg
      541 agttaacatc attgttaatc aattcgacca gtcagtaaat atgctgactg gtcgaacata
      601 atttgctaaa aagtcatcac tgactgatgt cagtcccacc atctgccagt tcaatctacg
      661 ggtatttcca gcgtatcgcg gcacgcgatt gctggtctgt tgatgtagtg gattgtagtt
      721 gctgactttt cttaccctca gatttctgcc ccacttgtca attcaatctg ccaataaata
      781 acataattat atttttattc ctcacagaaa ttccttttta aaaatagcga acccactgta
      841 aaaaacactt tttgtatacc tctaccaagc gtaataaaat aaacgctcat aattcattat
      901 aaataataat gaaataacgc cttttattga taatttacat atgaaaaaat ttttcctttt
      961 gttatattat ttctgttttc actccctctg attattttta ccaaccctgg gtcactgcat
     1021 tatcaccaac gccccgacac gtaagtcctg agcaattaga aaaaacagtt cgttatctta
     1081 cacaaactgt gcatccacgt aatgccggca atattgataa tctgaatagg tccgcagagt
     1141 atataaaaga gttctttgtc agtagcggtg ccagagttac cgcgcagggc gtccccgatt
     1201 ggctattacg actctgcgcc tggaagccag aattaccctt acccggcaat gtcctggctt
     1261 tatcccgatc ggggagactt tattgctgtg gtcggcagaa tgcaggatat caacgccgtt
     1321 cgtcaggtaa aagcggcatt gttgtcatcc cgggatttat ctgtttattc tatgaatgcc
     1381 ccagggttta ttcccggtat tgatttttct gatcacctta attattggca acacgatatt
     1441 cccgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt
     1501 gataccgcag acagattgaa ttatcagaaa atggctcagg tggtagatgg tgttataacc
     1561 ttgttataca acagtaaata ataaaaattt ctcgaggcgg gtccagaaac agcgacatgt
     1621 acattttatt ttccctcgtc tgttttaaga tgcgaaacat aaaattcaat ggaggaaaaa
     1681 taaatgggaa tgattggcta ttttgcagaa attgactcag aaaagattaa ccaactgctt
     1741 gagtctactg aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga
     1801 ctggatattg ataaacgctg ggatttttta cactttgggc tgacaggcac ctctgctttt
     1861 gatcccgcga agaatgatcc gctgagtcgt gccgtgcttg atgaacacag cctcgaagat
     1921 ggcatagatg gcttcctggg attgacctgg aatcaggaat tagccgcgac aatcgaccga
     1981 ctggaatcac tggatcgaag tgagctgcgt aaacagtttt ccattaagcg actcaatgaa
     2041 atggagattt atccgggagt gaccttttca gaagagttag aggggcaact ctttgcctcg
     2101 atcatgcttg atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat
     2161 catgcgctga cggttattgt tgggtgatgg tgtcagagcg ccaaaaaaaa ccgccgaaca
     2221 tgtcggcggc tttctgactt acaaactaac agtttacatc agcggcattg ctaaccgcac
     2281 aatactaatc agcggttgtg gccatacacc cagcaccagt accaacagtg cagaaatcag
     2341 caccacgata ccgcccgcgc tgtactgcca gtttgacggt gcatcgcgac ccggttgttc
     2401 cggtgcgtgc aggtacaggc tcaccgccac gcgcaggtag tagtagaggc cgattgccga
     2461 accgacaacc acggcaccca ccagccacca caagtgtgcc tggacaccga ccgccagcac
     2521 gtagaactta ccgataaagc ccagcgtcat cgggataccg gccagagaca gcatcatcac
     2581 cgtcatcact gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga
     2641 atcagcatcc gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag
     2701 gctgctgaac agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccggt
     2761 ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag
     2821 cagacgtttg atgttggtct ggctcaacgc catcaggtta ccgaagatga tggaggcaaa
     2881 ggcgataatc gccagtacta cgcgaatcgc ttcgctgcca cccaccggtg cgtagaggaa
     2941 cagacgcatc accacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac
     3001 cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag
     3061 tttgaagccg aggccaacaa tcatcatgcc gaaacctgcc agcaacagcg gctcgttgag
     3121 cataccgtcg ccgaggtttt tacccagcgc gacaaacgac aggtcgccag actgcgcata
     3181 caccagcgcc ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt
     3241 gatactggct tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg
     3301 caaagagatc agttcaatac cgaggaacag agacgccaga tggtttgcat tcgccagcag
     3361 gatcccgccc agcgcggcaa ttaacaccaa caggtagaac tcatccttgt tgtcgttata
     3421 gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc
     3481 ggtgtaaagc atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc
     3541 ctggccaaca aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc
     3601 gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc
     3661 gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta
     3721 cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg
     3781 cgaggtatcc agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa
     3841 caggatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct
     3901 tttcgcttta ccgaagtaag cgcgatgcaa catcgccagc gaataaacag atgcaaagac
     3961 cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat
     4021 cataaattcg ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa
     4081 cagcgacagt gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc
     4141 gcgggtatgg atacgttcat aaagctgacc gcagagaata aacagacccg ccgccgacaa
     4201 gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat
     4261 tgcaatcagc acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc
     4321 ggtctgagcg aaggccatcc acgcaccgta gaagatgccg ataacaccca gccacatagc
     4381 gattggcgcg aactctgccg acgcgttcgg gaacagcggc agggagaaac gcaacaaacc
     4441 gtaagcggcg gttttcagca agatccccgc gaggtccacg gaaccggcgg tcggagcctg
     4501 ggagtgcgca tccggcagcc agccatgcag cggaaccacc ggcattttga ctgcgaaggc
     4561 gatgaagaag cccagcatca acagatattc cacaccactg gacattggcg tattcagcag
     4621 ctcttcatag ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag
     4681 gatggcgatc aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccac
     4741 cgtgatacgc gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat
     4801 cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac
     4861 gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc
     4921 gatctctttc cacgaacata gtaccgccag cacaccgagc agaccggtca gcacgaccat
     4981 cagcagcgac agaccgtcaa tggcgagatg aatagagata ccaaaacgcg ggatccacgg
     5041 catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc
     5101 ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca
     5161 gcgtggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc caataaaggg
     5221 aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag
     5281 gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg
     5341 gatgcgccgc gaaggcagct tatccgacct acgttcttat atgcccaggg gcaatcccac
     5401 aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca
     5461 tggatgccac ataccagcgc agataaccgt tctcgcttaa cagcagacct ttacctgcaa
     5521 agcgggaaag aaccgccggg atgttcatca ttgagttcag cggatcgcgt ttcagcaacc
     5581 aggcaatacc caggaacggc ttgacgaaca ctttgtcgta cagccagtca aagccccagg
     5641 cgttgtacca ccaggtactc agcaggcggc ccggcgcact gttggcgatg gaggtcacca
     5701 gagtacgttt acccagccac agccaggctg ccagcagaat gccgaccacc gcgaccacgc
     5761 cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca
     5821 cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa
     5881 tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt
     5941 ggaagacgat gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac
     6001 ctgccaccat cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac
     6061 tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc
     6121 agagataaac cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt
     6181 gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt
     6241 gagtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt
     6301 agccaatctg gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg
     6361 cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat acccaccaga tgcagaactt
     6421 ccggcgtcat caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca
     6481 tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcagcaagcc
     6541 atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg
     6601 cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc
     6661 ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt
     6721 cacccacacg ggtcacgacg aacgctttca ttgctgctgc gccattcttc ggatcggtgt
     6781 aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca
     6841 gcagcaggtt atcggcgaga accagaacta ccatgctggc gatgaacagg ttggtgtagg
     6901 cgaagaagcg agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa
     6961 ggaaacccac accagtgacc accgagagca tggtcagcga caggccgtcc agcaccaggt
     7021 taaaaccgat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct
     7081 gctcgccgtt agcgaagaaa tcaacgccga taaaggcggt taccagcgcc gccaggccca
     7141 cagagccaac gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca
     7201 gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccacgcat
     7261 ctcacttact gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc
     7321 aaggccgata ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc
     7381 gtcggtctga ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat
     7441 gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc
     7501 aagaacgaat aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc
     7561 tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct
     7621 tcacgaccga cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccagttcc
     7681 accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta
     7741 ccgtcgatac cctgatcgtt aacaccgagg atggcgtaaa caatcaccac cagcatgatg
     7801 gccgacaaaa ttgccgggcc aatccacacc tgcggtttca gccactggcg ttcctgttcg
     7861 atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca
     7921 cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag cgcctagtga gaagaacacc
     7981 ccggaaatcg ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg
     8041 gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc
     8101 attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg
     8161 cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg
     8221 ggtatttgcc cggaccggag atcagcagat cctctttctc gtaaaccaga tcctggcgct
     8281 tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt
     8341 cgcacagacc acagaaaatg cagcgtgaga agttaatgcg gaaaaattcc ggataccagc
     8401 gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcac
     8461 agaggttaca ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac
     8521 cacgataacg gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg
     8581 cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca
     8641 attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc
     8701 cgttaccagc aaattgatca gcgtcagcgg caggcagatt ttccagccga aggacattac
     8761 ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa
     8821 cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa
     8881 gaacagcgtc accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa
     8941 cagaccgaac ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc
     9001 ttccggctgg tcaaacgggt gacggtgaca caccgccacg cccgcgatgg caaaggtaat
     9061 aaaaccaaag aattgcggga tgacgttcca cacatgcgcc tggctgttga cgatgtcggt
     9121 catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc
     9181 gtagctcagg gtctgcgcag aagcacgcat cgcacccagc agcgagtatt tgttgttact
     9241 tgaccagccc gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa
     9301 aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa ttgcaaaggc
     9361 cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa
     9421 tttcgggatc cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga
     9481 accgccccag ccaacacggt taggtccata acggttctgg aacagaccca gcaggcgacg
     9541 ttcgccaaag ctcatgaatg ccccacaggt gacaaccacc agcaggatcc ccaccgcttt
     9601 gaggatggtc agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc
     9661 ttgagatcct caagatgcgc gccagccagc accggagcaa tgccggacat acccatcggc
     9721 aagcccacct gccctgccgt cagtccttcg gcgatttcaa ccggcagcgt gaccgtgttg
     9781 ccatcgtaac taaaggagac gcgtgtacct gcgttcacac ccaacttcgc ggcatccgct
     9841 gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac
     9901 aattcatcgc tgccaaacag gtggtagtac ggcgcgatac gccatttccc gtcctgcggc
     9961 tggaagcgtg ccggtacgct ggtgaagtaa tccagaccgt tttcgctggt ttcaaacaga
    10021 cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac
    10081 gcctgcgggg agttccagcc cggcgcccag gcaaacggca cttgcgaacg gtgcgcggtc
    10141 ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc
    10201 ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca
    10261 cgggccagtt tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgataccc
    10321 gccagttccg ggattttcgc cacaacagcg tcaattacat ggtcgagctg cgtccagtcc
    10381 acttcacggc tcagcaaggt gctgtgcagc gagtgcagcc agcgccagct ttccagcatg
    10441 acagttttgc tgtcgtaata agccggatcg taaacctgga agaaacgttg ggcgcgacct
    10501 tcattattaa ttaccgtacc gtcgctttca gcaaagctgg cagcggaaag caccagatgg
    10561 gcgttttcca tgatcgctgt gcgttgatga tcaaccacca tcaccagcgg cgctttagcc
    10621 agcgcagcat tcacgcgggt agcagaagcg taacgatgca gatcgttttc caacaccacc
    10681 accgcgtcgg cgcgtccggt ttccagttcg gttaacgctt cttcaagcga accgccaccc
    10741 ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc aacgtcagcg
    10801 ccgcgacctt tcagggcttt cgcgacgtta gccgcagcct gaatcacctc tatgctaccg
    10861 gcgttcgtcc cggagataat caacggtttc ttcgcacctg ccagtgcctg cacgatgacg
    10921 tcgattttgt tttgcagctc aggttcgata ccgtcaaccg ctggtgcgga gttatccagc
    10981 gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac
    11041 gccgcgatat catccagacg agtgtcatca acgttggtaa caaacagcgg atgcttcgca
    11101 cgttgaccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgcagcc
    11161 atttcgcgcg ctttaccttt caccgcctga cgcactgcca gtgcgacgcg cgcgccggtc
    11221 tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc
    11281 ggagtataaa tgccgccttc acgcagcact ttcagcgcca gttgcagacg ttcctgctca
    11341 ccgtgagcga taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg
    11401 ctttccacgc tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata
    11461 tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc
    11521 tgtactggct gacgcggacg atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg
    11581 tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg
    11641 ccgtaacgtt caccggggct gatgttacag ccgatggaac attgctggca gatgctcggc
    11701 gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg
    11761 ccggtcgggc aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc
    11821 gggcgaccga agtagacgtt gtcgtgcgcg ccataaacgc ccagatctgt accgtcagcg
    11881 taatctttgt agtaacgcac acagcggtag caggcgatgc agcggttcat ttcgtgagag
    11941 atgaatggcc ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag
    12001 ctgtgtccgg tcatcacggt catatcctga agatggcagt taccgccttc ttcacatacc
    12061 ggacagtcgt gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc
    12121 gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc
    12181 accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa
    12241 gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac
    12301 agacaagctt ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga
    12361 attgtagcca ttagcatgct tccagttttc tcggtcaggg accgagcgtt aatcgaaatt
    12421 cggttaccag cgctctttca gcaggttcgg ctgaatccca ttaatcaaat gggtattgct
    12481 gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa
    12541 cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca
    12601 cagttgctca agtgtttcga tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag
    12661 aattttcacg ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg
    12721 ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc
    12781 catcgccagc gccgtaccca gacggctgcc cgctttaccg atactttcga attccatcgg
    12841 cagatcaagg tgcgcttcgg tcaggaagtc agtccccgcg ccgcctggct gccaggcttt
    12901 aaatttcaga ccatcacgca taccaccggc gtaatcttcg aggatctcgc gtgcggtggt
    12961 gccgaacggc agttcccaca gtcccggatt tttcacccga ccggagaagc ccatcagctt
    13021 ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat
    13081 cgccggaacg ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc
    13141 agaggttgcc gggaaggggg gtttcgagcg tgggttagca cgacgccctt ccagggagtt
    13201 gattaacgct gtttcttccc cgcagatgta gcgccctgcc ccggtatgga cgaacagttc
    13261 gaagtcgaaa cctgttccca taatgttttt gccaagcaag cccgcttcag tggcttcggc
    13321 aatagcgcgg cgcagattta ctgccgcttc gatatattcg ccacgcagga agatgtagcc
    13381 acggtaagct ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag
    13441 ttgctccatc aacaggcggt ctttataggt gcccggctcc atttcatcgg cgttacacag
    13501 caggtaacgg atgttcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga
    13561 aaagcccgcg ccgccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc
    13621 cggagacagc ccggtcagcg ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc
    13681 gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg
    13741 agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc
    13801 agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg
    13861 cagcaagttg gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc
    13921 agctttttct cgagcgccgc ctgaataccc tgatacccgt tgatatgaca gaccacgctg
    13981 tcgcaataac ggatcacatg gcgaccaacc ggctggcgga agatctgact gtagaacgtt
    14041 gccacacctt cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca
    14101 ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca
    14161 cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc
    14221 tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca
    14281 taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaagctg ccgcggatcg
    14341 ccgccggaat ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca
    14401 tggtgctacc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct
    14461 ggaaagattc attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca
    14521 gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg
    14581 ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc
    14641 gcagctcttc cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg
    14701 ggatttcaaa gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt
    14761 cgatcccggt agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc
    14821 cataggcggc aacgccctgg gtacgacctt tcagaatggt attttgcagc gccgctttct
    14881 cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcaga cgatcccagc
    14941 cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac
    15001 ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg
    15061 gcgtcattgc gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg ctgttgatac
    15121 ggaacagttc ggagagcata acgcgaatga cgttaacgcg atccggcaca gtgatcccgg
    15181 ccagtttctc taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcaa
    15241 tacggtcggt atacggaatg tagctgtgcc aggactggcg ctcgcccatt ttctccgcac
    15301 cacggtggtg gtagccgatg tctggtacgc agtcgacaat ctcttcgccg tcgagttgta
    15361 aaacgatacg gaaagccccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga
    15421 agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt
    15481 ccatctccag atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg
    15541 ccgggtaatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca
    15601 ggttcgggtg accgtcgaaa gtaatgccaa acagatccca ggtttcacgc tcataccagt
    15661 tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca
    15721 ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat
    15781 ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa
    15841 acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc agtaattgtt
    15901 cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg
    15961 gcccaaaacg gttgcgcagt tcgccaatta ccggatcatc aagatgatcg cgggtctgcc
    16021 aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt
    16081 gactgtcggc gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt
    16141 tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt
    16201 caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa
    16261 ccacccagga gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct
    16321 gcatgtacgc ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt
    16381 tatcgacgcc ctgcacaacg gaataaatat cgtacatacc accagagttg gcacaggccc
    16441 ccattgagat aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg
    16501 ccattttggt aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac
    16561 gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct
    16621 caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt
    16681 taaccatgtc attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt
    16741 cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc
    16801 gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt
    16861 tcgtttccgg gttcatacac tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa
    16921 tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa
    16981 agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa
    17041 cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg
    17101 cggagccgac cgaatcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc
    17161 gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa
    17221 gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg
    17281 cttactcatc aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg
    17341 attcatgggg aggaataaaa aaaaccttac aatcactgta gaaattcttt tatacagcta
    17401 attgatgtgg tcttttactc ctttctataa ccttttgtca actttaacaa aagtttcttc
    17461 acattagttt acataatatc aacaccatta gcatttaatg ccctttcacc ccagatcctt
    17521 gacgactcca ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa
    17581 tacgggtcta ttttgacagg aatttgtgtc gattcctccc cccaaaagag agtattttct
    17641 tgatctgtga cacgcttttg ttattccata acaaaaacgc agcaacaaat ttacgtattt
    17701 tttaacatca ttgtagcagg tgattttttt caggcgatta tttgtgcgtt cgggacgtga
    17761 atctctggtg attgaaaaat aaacagtttt gtacgttctg cactatgcga ggaaggattt
    17821 ttactaaaaa aaaagccgct ggggtttaaa acacccccag cggctcgttt tttacactat
    17881 tgtctcagga attatctatc gtccgtcgat tactcgatat ccctttcaat caacaatgaa
    17941 tcatcccctt ccggagcaga cattgggctg tattgccacg gattatggta gctctccatc
    18001 gcctgataga tcacctgggc cagctcatta ttactggacg ggtcatagca cagcaaatat
    18061 tcggtatcag gtagcggcgg taagccatct actccactca gcacacgcag gtccgggctc
    18121 atcatctcaa ccggccttgc cgtcacgcca agaccggctt tcactgccgc acgaacggcc
    18181 ggaagcgtcg aggcgacata agccagtcgc catggaatat ctgctttatt aagcgtcgcc
    18241 agcaccatat cgcgaaacgg acttggatca tccagcaata caagagggat cggctcgcct
    18301 ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat
    18361 gccttaaacg ctgaaggacg atgggtggtt accatcaaat ccacttcctg ggattccagc
    18421 atttctgcca tataggcatt acgtttaacg cggacatcca gcgctaattt cggataaacc
    18481 gaactcacgc gatttaacag gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg
    18541 atagttaaca cgccctgaag attactgaac attaatgatg agcaggcctc atcattaaaa
    18601 cgcaggattt tcctggcgta accaagaagt tgaatgccat gttcagttaa cagtttgttg
    18661 cgaccgtgac gagcgaacag ttctttccca acgagttgtt ccagacgctg catttgctga
    18721 cttacggcgg actgagtacg acacacagcg gcagctgcgg cagcaaaagt gttcagatcg
    18781 gcaacagcaa caaatgttct cagcagatcg aggtcgaggt taattatcgg acgatttgca
    18841 cttatcatat attatcactt actggcggct catactgagc tggttaatgc tgtgcacaca
    18901 caaacaagca attccatttg taatgtgcct ccctggcagt ttcatccgga acccggacga
    18961 aagtaaaaat gcatatgagt tgcactaaaa aagcgactca cattgttccg ttataatgcc
    19021 tgaagtagat cacagaatat atcttcaggg atcgcatatc tattaagtta ctcactcttt
    19081 ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag
    19141 catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaagcggcat caagagcagg
    19201 gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg
    19261 aattatctga tgcaaagtta tgttatgtta tgttatgtta ggcaaagtaa tcttctatta
    19321 ttaataagca catcaaaacc tttttgaata ttaaataata attaattagc atcatcctca
    19381 ttcattaatt ccgcttaaca atagtttcac accttacccc cattgcgaca cctcggaaat
    19441 catcacgctg ttaaactctc cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat
    19501 tcttaaatga ggcattttca cacaattatc ttacagataa aaaaaccata cttgcaatta
    19561 agaactagaa caattcacta tataacatta catgtaaagc atatacacct cattattttg
    19621 tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat cttattctgc
    19681 agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc cacggcaggg
    19741 agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat
    19801 tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag
    19861 aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccattc ggttttgacg
    19921 cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa gggtattgcg
    19981 attccaaagg gctttactcc gcgcgtaaag ccatcatgca gcactaccag gctcgtggca
    20041 tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag cttatcgttc
    20101 aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca ccagattacc
    20161 cactctggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg
    20221 aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg cctcgtacgc
    20281 gtgggattgt tattatcaac ccaaataacc caaccggcgc ggtatattcc aaagagcttt
    20341 taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc gatgaaattt
    20401 atgacaaaat tctctacgac gatgctgagc atcactcaat tgcgccgctg gcacctgacc
    20461 tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg
    20521 ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa
    20581 tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc
    20641 tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt tatgagcagc
    20701 gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg
    20761 gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcat gacgatcaga
    20821 aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca
    20881 actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc
    20941 tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa tcttaatttc
    21001 actgccggag attgcatccg gcagtgttat cccgccacaa tgacctgatg atgtcatcat
    21061 acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc tgaaactcat
    21121 taaccgctgg ccgctaatgc gcaacgtgcg gacggaaaat gtgtccgaac acagtttgca
    21181 ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cggaaatttg gcggtaatgt
    21241 caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag tgctcaccgg
    21301 cgatctccct actccggtga aatacttcaa tgcgcaaatc gctcaggagt acaaggctat
    21361 tgaaaaaatc gctcagcaga aactggtcga tatggttccg gaagaactgc gggatatctt
    21421 tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc
    21481 agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa ataatgaatt
    21541 cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc aggagatgga
    21601 ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga ttagccagga
    21661 ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca
    21721 tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac cgccatcacg
    21781 ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt tcccggacct
    21841 aaaaccaggg tgttaacagg tgaagacacc ggtgtcataa aggcggcgga tgctgccatc
    21901 gccacgacca tcgcaaatgg ataaggcgac actcccatcg ttttggcagc agccagcgca
    21961 atcggagcca tcaacaccgc cgtcgcggta ttagagataa acagcccaat aaccgccgac
    22021 aagacaaaca aacaccccag catcatatgt ggcccataac cgccgccaat atccatcagc
    22081 cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc
    22141 ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc agcatctata
    22201 cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt aggaatttca
    22261 tttgtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg tgcctggctg
    22321 tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc
    22381 tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat atcgcccagc
    22441 agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt gagccccacc
    22501 acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc aatcagttct
    22561 gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc agaaaaatac
    22621 tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg gagatcgaca
    22681 tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta
    22741 acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac gttagcgcca
    22801 taacgctcac gtaatttgag atcatccagc cgttgaccta tcattggcga tccggggcga
    22861 atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc gcgaaaggtt
    22921 cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc
    22981 actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt tacgctaaag
    23041 aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag gttcggcggc
    23101 gtcgccacca gcgtcatcat gccgctaatc agcccggcaa aacttaacgg catcatcaga
    23161 cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat aaagatagcg
    23221 acaacgctgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat caacaaaacc
    23281 aacattttga tttcactgtt gcccgccact ttgaccagcc atgttcccat tacggtggca
    23341 acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag gacaacgtta
    23401 ggatcagaaa agccggaaaa tacttcgggg accgtcagcg ttccgcttaa tgcaaacgcg
    23461 acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa caagacgatg
    23521 gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta tccttgttaa
    23581 ttgaggggga tgacttgatt ctgccataaa aaagccccga cgagacgggg ctaaatcatg
    23641 atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca gtaatttgct
    23701 ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa
    23761 ccgcaatgac atggcaaccc gccgctaggc cagaaagcac gccagcggga gcatcttcca
    23821 ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc acctaacaga tacgcatcag
    23881 gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc
    23941 ccgctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg
    24001 gaatacctgc tttattcaaa tgattgagta aggcgattgc cccaggaagc gcggtaatac
    24061 cttcggtttc cgtggcctcg atttgttcca gacgcgtaaa ctcggcggca atatcagcct
    24121 cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag
    24181 ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac gcccgttcta
    24241 ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca
    24301 cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa atggtactga
    24361 cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg
    24421 gcgttaaaac catgagtccc agcgtcaaaa tgggtatagc gcccttccac attaaccata
    24481 aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca
    24541 cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga
    24601 tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg
    24661 cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg caggcgacgg
    24721 taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata
    24781 agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactctact acggtgaaaa
    24841 aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc
    24901 aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta
    24961 atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac
    25021 ttcataaaac cagttaagga ttgcaggggg taatggcgtt acagaacgct tgcccagcca
    25081 ccacaatccc tgcatgggta aacttaaggc gaacagtgca gtggcaactg ccggcccaag
    25141 cagaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg
    25201 gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt
    25261 ttccagtggc cacgtctttg agtaatgctg tccgcggcga aacaagctaa aaaaattaac
    25321 agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat
    25381 ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact
    25441 atcaggtatc ctttagcagc ctgaaggcct aaatagtaca tattcattga gtcgtcaaat
    25501 tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc
    25561 ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca
    25621 tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc
    25681 catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat
    25741 catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc
    25801 cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg
    25861 cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga
    25921 actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac
    25981 cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc
    26041 accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca
    26101 gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga
    26161 gtcttacctc tacgccctgc cgtacaacct gtacaaagag cacggcatcc gtcgttacgg
    26221 cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc
    26281 ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat
    26341 ccgcaacggt aaatgcgttg acacctctat gggtctgacc ccgctggaag gtctggtcat
    26401 gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg
    26461 catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac
    26521 cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa
    26581 gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct
    26641 gatggatggt cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat
    26701 ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg
    26761 caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc
    26821 ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc
    26881 ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt
    26941 aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc
    27001 ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg
    27061 agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg
    27121 actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac
    27181 gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac
    27241 taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt
    27301 aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc
    27361 gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg
    27421 acccgcaaca gcttcggtgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa
    27481 ctgaacgcac cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac
    27541 gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg
    27601 ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat
    27661 atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt
    27721 aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt
    27781 tctctgctgg tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc
    27841 atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc
    27901 atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc
    27961 aacacctggc agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat
    28021 cacgaacgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc
    28081 gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgccagc gttccgttat
    28141 cagctgactg aactggcgcg caaagcgggc aaacgtatcg ttctgccgga aggtgacgaa
    28201 ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg
    28261 ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actaggtgca
    28321 ggcattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgag
    28381 ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg
    28441 gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt
    28501 cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc
    28561 tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac
    28621 tgtgcgatca acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct
    28681 gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt
    28741 acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa
    28801 aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac
    28861 gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc
    28921 ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc
    28981 tccatcgggc cgatgctgca gggtatgcgc aaaccggtta acgacctgtc ccgtggcgca
    29041 ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag
    29101 taatctcgtc atcatccaca gctttgcgct gcggatatct gaaccggaaa taatcactat
    29161 ttccggtttt ttattctctt aatttgcatt aatcctttct gattatcttg cttaactgcg
    29221 ctgcatcaat gaattgcgcc atctcacttt gcatacttac cactttgttt tgtgcaaggg
    29281 aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc
    29341 gatacgttgg tgattatctt ttttgttgct attttaacca gtcttgccac ctgggtagtt
    29401 ccggtgggga tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca
    29461 cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag
    29521 tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg
    29581 tttgaagggt taacctcagg atcgaaatac gggacagccg ttggcatcat catgtttatg
    29641 ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc
    29701 ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt
    29761 ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt
    29821 atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgact
    29881 tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggttgtt
    29941 gctcagggga ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg
    30001 gttatcgcca ctctgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag
    30061 aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat
    30121 gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct gacagccgta
    30181 atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc
    30241 cagttattca ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc
    30301 atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct
    30361 gccctgctgg tgggtttcgc caaagggatt ttgctgctgg ttggtaatgg tgaagcaggt
    30421 aatgccagcg tgttaaatac tatcctcaac agcattgcca atgccattag cggtctggac
    30481 aacgcggtcg cggcctggtt tatgttgctc ttccaggctg tatttaattt cttcgtgacg
    30541 tccggttctg gtcaggcggc attaactatg ccgttactgg caccgcttgg cgatctggtc
    30601 ggtgttaacc gtcaggttac cgtgctggcg ttccagtttg gtgatggctt cagccacatc
    30661 atttacccaa cctcagcttc gttaatggca acgctcggtg tgtgccgggt ggacttccgt
    30721 aactggctga aggtgggcgc gaccctgctt ggactgctgt ttattatgtc cagcgtcgta
    30781 gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc
    30841 ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac
    30901 aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc
    30961 ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag
    31021 acgcaactga acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg
    31081 tgctcggcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat
    31141 tccagcagtt gctcatcggc ctgaactaca ccgcctgcgg tcgcatctaa catgccgggt
    31201 aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg
    31261 acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt
    31321 gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc
    31381 tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg
    31441 gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca
    31501 ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt cggaatactc
    31561 accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta
    31621 tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga
    31681 tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt
    31741 atcgggaaat gcagcatcat ttgatccact tcgctgtcgc agttgccacg cacagcgata
    31801 accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt
    31861 aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg
    31921 ctttggacaa acaactccac aacacgttcc gtcgccggta acgacccatg aatgtccgat
    31981 gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt
    32041 ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc
    32101 tcgctgccac tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag
    32161 taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa
    32221 attcggctga cccagtgcta acaaatgttc tgccatcgcg aacagtttct ctgcactggc
    32281 ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg
    32341 ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc
    32401 acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc
    32461 cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg
    32521 cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag
    32581 gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aggcggataa
    32641 cacataaggg gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat
    32701 gtcctcggca ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt
    32761 gacgaatact cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca
    32821 accggagctg caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg
    32881 ctgtttctcg aagaagcaga gcttgattat cgcttgatta aggtagacct ggggaaaggc
    32941 ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt
    33001 gatcattctc ctgccgatgg cggcgaaccg ttaagcctct ttgagtctgg tgccattttg
    33061 ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc
    33121 acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat
    33181 cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa
    33241 actcagcgtc tttaccatgt actaaacaag cggctggaaa actcgccctg gctgggaggc
    33301 gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgcctg
    33361 cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc
    33421 cctgccaccg ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa
    33481 cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg
    33541 cacaacctgc cgctattatt cgtataaaga accttcgttt gcgtacgttt atcggaatta
    33601 aggaagaaga aattaacaac cgtcaggata ttattatcaa tgtgacgatc cactaccccg
    33661 ccgataaagc gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga
    33721 aaaacattat tcagcatgta gagaataacc gtttctcttt gctggaaaaa ttaactcagg
    33781 atgtgctcga tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata
    33841 aactgcacgc gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaat
    33901 cgccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg
    33961 ccatttgatt ccgcgtttgc tggagctggg ccatcaaatt acggtagtga cgcgtaaccc
    34021 gcagaaagcc agttccgttc tcggccctcg ggtgacacta tggcaagggc ttgccgatca
    34081 aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa
    34141 acgctggact cacaagcaaa aagagcgtct ctgccaaagc cactggaata tcacgcaaaa
    34201 actggtcgat ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc
    34261 aacgggctat tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa
    34321 cgaatttact cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga
    34381 caaaacgcga gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct
    34441 cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca
    34501 gtatctggcc tggattcata ttgatgatat ggtcaacggc attctctggc tgctggataa
    34561 cgagctgcgc gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc
    34621 ccatgcgctc ggtcatgcgc tgcatcgccc ggctattttg cgcgtcccca caaccgccat
    34681 tcggctgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa
    34741 acggctggaa gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga
    34801 tgtcgttcgc tgatgtggtt tacagcaaac atccgccagt taactccctg tgttacagga
    34861 ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc
    34921 tctgtctaaa cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc
    34981 ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg
    35041 tggtgcgcgt tctgttatct caccaataaa tgcacggaaa cgctgggcat cccctgtttg
    35101 taatacgtaa ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag
    35161 cgaaacaatt tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa
    35221 ctccaacagc gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc
    35281 caccaacgga aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc
    35341 aagccagcag agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat
    35401 caccaatgga agctctttat agcccgcatc aagatggttt tgcattgccg caatggaata
    35461 acgcatcatg cgaaaagcca ttaattcttc cggcttactt tggtgctcta tcactacata
    35521 aatataaccc actccctcct gcgttttcac agaccacaag aggtcggaat aatattgccg
    35581 caggtcttca tcaataaaac tgtttggttc tagtttaagc gtcgttaaat cacacagttt
    35641 gcgcagcggc gcgggaagat gaatattaat aaaattccgc gcggtgtctg gatggcgtaa
    35701 aaaagattta aataccgcat catgcggcgt ggaagttgtc gatattgtca tggcgttccg
    35761 tcacccctca aatttaagat gacgcgacaa taaccacgat taacacccaa tgcagcaggc
    35821 agtttttatc tttggacagc gaattcagaa gtatttttag cggactgcaa cttttacatt
    35881 tcagcggaag ctgactcgca aattcccctt gaaaatactc cggcagcgtt agccaccgga
    35941 gaggaaatta tttcagcgat cccttaagga actgttgcag acgagggctt tgcgggttgc
    36001 caaataactg ctccggcgcg ccctcttctt ctattttccc ctgatggagg aaaatgacgt
    36061 gagtagaaac atggcgagca aagcccattt cgtgagtcac taccaccatg gttttcccct
    36121 cttctgccag ttgctgcata atacgtaaca cttcgcctac cagttcagga tcgagcgccg
    36181 aggtaggttc atcaaacagt aaaacttctg gttccatcgc cagcgcccgc gcgatagaaa
    36241 cacgctgttg ctgaccgccg gaaagatgca ccggatattt cccctgcgca cgttcgtcta
    36301 tcccgacttt ctccagatac ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca
    36361 acacctgaat cggcgcttcc atgacgtttt ccaacaccgt catatggctc cagagattga
    36421 agtgctggaa taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga
    36481 ctttgagttg accgtctttg tcgcgcacca gattgatcgt ctggccattg accacgatcg
    36541 atccttcact cggtttttcg aggaagttaa tgcagcgcag aaaggtactt ttccccgatc
    36601 ccgacgatcc gatgatgctt attacatctc cggcattcgc ttgcagtgag acccctttca
    36661 gcacttcgtg ttcgccatag cgtttgtgca aatcgataac gtttaattta ttctcggaca
    36721 tcgtattctc agtgcgttga agaaggtttc acatgctgca accagcgttt ttccgctctg
    36781 cgaaagaggc tgatcaggac ataagagatg attaaataga gcaccgcggc aatgccgaag
    36841 gcggtaaaag gttgatacgt ggcggcgtta atatcgcggg ctattttcag caaatccggc
    36901 acagtggccg taaacgccag cgcagtagag tgcagcatca ggatcacttc gttgctgtat
    36961 gccggtaacg caatacgcag cgcagaaggc aaaataatgc agcgatacat tttaaaggtc
    37021 gagaagccat aggctctggc ggcttcaatt tccccatgcg gaaccgaacg gattgcccca
    37081 gcaaaaatct cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc
    37141 aggccactgc ggaagaaagc gttaaggaat tcggttccct taacaatctc aagcgtgtac
    37201 atgccggaat agaacaccag caactgaaca tacagcggcg taccgcgaaa aatataggta
    37261 aataaccaga ttggaaactg gatgtattta ttactggaga cacgaccaat cgccagaaac
    37321 agcgccagga ctccgcctat cactaccgac aaaataagca gccacagagt gatcgccaca
    37381 ccagtaaagc gataaccgtc ggtccacagc agcggtttcc agtattcatg taagatttcg
    37441 atcacaggtc agccctcttc acacccacgg agtagcggcg ctcaaggaac agcagcacac
    37501 cattggaaac ggtggtgaaa accaggtaaa tcacgccaca gacgatggcg aaatagaacg
    37561 gttcccaggt acttttgcct gccagctgcg tggctttgac cacatcttcc aggccgagta
    37621 acgaaaccag tgcggtagat ttgaggatca cctgccagtt gttgccaatg cctggcaggg
    37681 cgtaacgcat catcgccgga aacatgatcc tccgaaacac ttgcccgcga gtaaaaccga
    37741 acgccgtcgc cgcctctata tgccctttcg gcactgccat aaacgcgcca cggaacgttt
    37801 cggtaaaata agcaccgtag ataaaaccga gagtgataat accagcgacc atcggatcga
    37861 tatcaatctg cccgacgccc atcgcctccg tcaccatgtt tagcgcaatc tgcaaaccat
    37921 agaaaatcag cagcatcaac accaagtccg gcacgcctcg aatcagcgtg gtgtaccctt
    37981 cgaaaatcag ccccaaaagc cgattttgcg agagcttacc gccagcgcca attaaaccga
    38041 tgattacagc gagcactaca gagctgatag ccagctccag cgtgacgagc gcaccctgta
    38101 aaataacacc tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc
    38161 gcctcttgtc tccctccggt tacccgaagg gagcgggctt tctttcaccg atggcgatta
    38221 accaccataa acatcaaaat cgaagtactt tttcgctaat ttctcgtaag taccgtcagc
    38281 gcgcatttcg gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag
    38341 gcccatgccg gtccctacgc caaacagttt ttcatcttta acagacggac caccgaattt
    38401 gtaatcttta ccgacgggtt gtttgaggaa accttcgctg gcagcgacct catcctggaa
    38461 cgcggcatca atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc cctgatacga
    38521 gacgatttca atgccttttg gtgcccagtg ttcattaccg aacgtctcct gggtggtgcc
    38581 ctgcaatacg cctacccgtt tgcctttcag cgactcgact gtcggctgaa tgtcagaatt
    38641 tttcgccacc accagacgag aatcggcagc gtacagtttg tcggtgaagg caatttcttg
    38701 ctgacgtttt tccgtaatgg aaagcgatga catgatggcg tcaatcttct tcgcttttaa
    38761 ggacgggatt aacgcatcca gcgggttttc gacaaacgta cattgcgtat tgatgcgctt
    38821 gcacaactcc ttcgccagct cgatatcgaa accaaccagt tcgccttgtg aattttttga
    38881 ttcaaatggc gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa ttgcagcaag
    38941 tgccgcagtt gcgctggaga aggccagaac cagagagagc gatagcacca gttttttcat
    39001 aatgtcctca actgacagtc tttttatagg gaatatttac aggtttgtta cacttatcgt
    39061 gccataaaat tgaccatcaa ggcaaaatat cccgccccgt tagacgtatt atttttcatt
    39121 aaaaatgctt aagtatgcat ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc
    39181 atgatgatca ttccatcagg tacagcttcc cagcgacgta tcagtcaccg tagacattaa
    39241 agtcgaaata ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg
    39301 ccttattgaa ggcagccgtc agttcagcat catctttacg tagccctaca ccggtgccat
    39361 caccgaagta ttttttgtct tttactgatg agccagcaaa ggcgaaatct ttaccagcag
    39421 gttgcttgag gaatccttcg ctggcagcaa cttcatcttg taacgcagca tccagacgtc
    39481 ctgcagccag atcggaatag accaaatcct ggttggcata ggccaccaca tccacgcctt
    39541 tactacgcca ggtctcgtta gcgtaagctt cctgggttga tccctgcagc acaccaacat
    39601 gtttaccttt cagtgaatcc agcgttggct gaatcggtga acctttggca gcaatcaaac
    39661 gagaatctgc ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa
    39721 tggaaagcga cgaaataata gcgtcgattt ttttcgcttt cagtgagggg agcagcgcgt
    39781 caaagtcact ggcaacccag gtacatttca cctgcatccg tttgcacatc tcgttaccga
    39841 gatcgatatc aaagccaaca aaatcacctt tagcatcttt cgacgagaac ggcgcatagg
    39901 tggtatcggt tccgatacgt accgtctccg gtagcgccgc atagctggaa gccgctgcgg
    39961 agagaccgac taataaagac agagcgagaa tcgacttctt catacataac cctcaagtga
    40021 aaatggcttt tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct
    40081 tatgccatct tgacgacaac agtaacattc aacgttaaat atgttaataa gacgttgcat
    40141 tattgtcctg aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat
    40201 ggcagaaaca aaatgtcgag gatttgatcg cggttgcaaa attgccctga aacagggcaa
    40261 cagcggagtt atgcgccctg ccagcgggca aagagatctt caggaagggt tattgcaaac
    40321 tggtcaagaa cacgattaac cgtctgattt atcacatcat caagggattg cgggcgatga
    40381 taaaacgccg gaacgggagg cataatcacc gcaccgattt ctgccgcctg agtcattaaa
    40441 cgcagatggc ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct
    40501 ttcagcacca catctgccgc acgggtcagt aagccatcag tatagctatg gacaatgccg
    40561 gacagggttt tgattgaaca gggtaaaatc accatcccca gcgtctggaa agaaccggaa
    40621 gagatgctgg cggaaatatc gcgcgcatcg agcgtgacat cagctaatgc ctgcacttcg
    40681 cgcagggaaa aatccgtttc gagggataag gtctggcgcg ccgcctggct catcaccaga
    40741 tgcgtttcga tatctgtgac atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg
    40801 ccgctggcac cgctgatgcc tacaatgagt cgtttcataa atagttgccc tgtcagactt
    40861 gcaggcagac tttgcagtat ttcggcggga gttgcaagtc agggtgccag accggcaccc
    40921 tcagcgaagg catcatcctt cgttatgcat ttcgagattt tccacttcgt tctgacgttg
    40981 cactgctttg gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac
    41041 atctttggtg acgtagacgc cgttgaacac cgagcattca aactgctgga tatccggatt
    41101 ttcagcgcga acggcgtcga tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc
    41161 gatgatctgg cgaatttcat caacttcgcg accgtgagcg atcagttccg tggcgctcgg
    41221 catatcaata ccataaacgt tcgggaagcg aatttccggt gccgcagaag cgaggtacac
    41281 tttcttcgct ccggcttcgc gtgccatctc gataatctgc tcagaagtgg tgccacggac
    41341 gatggagtcg tcgaccagca ggacgttttt atcgcggaac tcggcgcggt tggcattcag
    41401 tttacggcgc acggacttac gacgcagctg ctggcccggc atgataaagg tgcggccaac
    41461 atagcggttt ttaacgaagc cctggcggta cggtttgccc agaatacgag caatttccag
    41521 cgcgatatca cacgaggttt ctgggatcgg gatcaccacg tcgatatcca gatcttccca
    41581 ttcgcgggca attttctcgc ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga
    41641 aattttgtcg ataaacgagt ccgggcgggc aaagtataca tactcaaaca ggcacggatt
    41701 gctgaccgga ttgtcagcac attgacgggt aaacaactgc ccttcttcag tgatgtaaat
    41761 cgcttcgccc ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga gcgctacgct
    41821 ttcggaagcg accatatatt ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag
    41881 cggacgaatc ccgtttggat cgcggaaagc aaccataccg tggccgataa tcatcgccac
    41941 acaggcatac gcgccgcgga ttaagcggtt tgtggcagca atggcagcga aaatattgtc
    42001 ggcttccagc gggtagtggc ggaagttgtc cagctcgctg gcgaagatat taagcagaat
    42061 ttccgagtcg gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa
    42121 ctcgtgagcg ttggtcagat tgccgttgtg ggcaagcgta atgccatacg gggagttaac
    42181 gtaaaacggc tgcgcttcag aggcgctgga gctgccagcc gtagggtaac gcacatgacc
    42241 aatgcccata ttgccctgca aacgctgcat atggcgagct tcaaatacat cgctcaccag
    42301 cccgttcgct ttacgcaaac ggaagcagtt attggcatct atggtgatga tgccggcggc
    42361 atcctgaccg cgatgctgaa gcaccgttaa ggcatcataa atcgactggt taaccggcat
    42421 aacaccggcg ataccgacaa taccgcacat acgtcttttt cctcgttaag ccacatctca
    42481 gagcacttac gctctgggca agaaacttga cgagctttgc agataatcaa aaaaccatct
    42541 gatgataaaa ctgaattgcg ggatcagctg tgatttgctc cagtcttcac ttttcgacac
    42601 cccggtaaag gagtcgagaa agaagagaat ggcggcaaca atcaacacac cgcgcaacgc
    42661 accgaaacag acgcccagca cccgatcggt gcctgacaac cccgttttct ccaccaactg
    42721 gcctatcacg aagttcacga tagcaccaac gatcagggta gcgataaaca gtaccgagat
    42781 ggcaatccca tttcgaacca gttcgtcttc aaagcccgta aaccagactg acaggtaagt
    42841 gtagtaatga ctggcaacaa agaaagcaca accccatgtc accagcgata acgcttcacg
    42901 aacaaagccg cggatcaggc taaccagaga ggaaaaagca atcaccgcga ttatggcgta
    42961 atcaatccag accatatgtg tcccacgatt ttacgccctg tcatcctgtt cggggcgcat
    43021 tctaacagaa aaagaaaacg tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg
    43081 cgctgaaaaa atattcaacg ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt
    43141 gtcggatgcg gcgtaaacgc cttatccgac ctacggttct aacactgccc aagcctgata
    43201 agacgcgcca gcgtcgcatc aggcaagact gtgttaattc ggcgtatagc ccattaccac
    43261 gccacttaag ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga
    43321 ggcatccggc ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt
    43381 ataaacccga taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt
    43441 tttcagcgca cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac
    43501 gaccggcttc ggttctggct tcggcgcagg tggtgcttca acctttggtt taggcggctc
    43561 caccggtttc ggcttcggtg gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt
    43621 ggctgcaata gtggccggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc
    43681 cgctgcgcct tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg
    43741 ctcatcacga tcgcccgctt tcggcaccag cgggatagcc gcgaactcat cctgataatg
    43801 ttttttctgc ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat
    43861 cgtgcccact aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt
    43921 ccatgacatg tgcgaccgtg tggaaagaac cacacaccag cacggtgtct tccgctttag
    43981 cgtccgccat tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca
    44041 aatgctcaag cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc
    44101 agtcatcaac cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca
    44161 taccgataac cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat
    44221 attccgccgc atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa
    44281 tctggaaacg tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga
    44341 cttccagccc gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa
    44401 gcggcaacgg cagattttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg
    44461 tgacggaata gttccactca acgccccgac gttgtaacag tgcacctttt tcctgcgcca
    44521 catcagcaat ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgga
    44581 agatgcctgc tttctcgcga ccaatacttt cgcgatctgg acccagccag tcggtatgat
    44641 ccagcgcaat actggttact accgcggcat cggcatcgac aatattggtt gcgtccagac
    44701 gaccgcccag ccctacttcc agaatcacca cgtcaagttg tgcctgcttg aacagccaca
    44761 acgccgacag cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa
    44821 tctccgcaaa agaggcggtg tgggccgatt ccggcaattc ccggccctgc acacgtacgc
    44881 gctcggtata acgcaccaga tgaggcgaac tgtagacgcc cactttgtac cctgccgcca
    44941 tcagaatcga ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa
    45001 acacaaatgg cgctggtttc aggacgccaa gacgcgccgc gacctggctc acgcgctcaa
    45061 ggccgagctc gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag
    45121 gcgacgcggc ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag
    45181 aatagcaaaa gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct
    45241 gatccggtac cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca
    45301 gattcatcaa cttcgccaga atgctcgcca gtttcaggcg catttccgga cgacggacga
    45361 tcatgtcgat cgcgcctttc tcgatcagga attcactgcg ctggaatcta ggcggcagtt
    45421 tttcgcgaac ggtctgttcg ataacacgcg gaccggcaaa gccgattaac gctttaggtt
    45481 cagcgatgtt gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt
    45541 cggtcagcac ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg
    45601 ttttcgccat ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg
    45661 agaagcagat cagcgggcag ttatcttcct gcgcctgctc aacggcacgc acgaaacgtg
    45721 cacccacaac agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa
    45781 ccggcattcc atacagagtg cctttcatca ccaccagcgc atctttttcg ccggtttctt
    45841 tctgcgcaga tgccagacgg tctttatact tcttggagtc acgaaacttc agctcatctt
    45901 tcggctcaag ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc
    45961 gattacgcgc tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt
    46021 ccagctcagc gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag
    46081 gaatgctcgc cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta
    46141 gggacctttc tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc
    46201 atttttgccc ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg
    46261 gataaaaaag tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg
    46321 tttgtgacgg attatttcga tgacgcccgg caaaatagaa accacaataa tcccgacgat
    46381 cagcagctta aggttatcct gaaccatcgg gattgtaccg aagaaatagc ctgcgtaggt
    46441 aaaaagcagt acccacaaca gtgcgccgat cacgttataa gcagcgaaat gacggtacga
    46501 catgtggccc attcccgcaa caaacggggc gaacgttctg acgatcggca caaaacgggc
    46561 gagaataatc gttttgccgc catgtttctc ataaaactga tgggttttgt cgagataact
    46621 gcgacggaaa attttcgaat tgggattact gaataacttt tcaccgaaca accgcccggt
    46681 agtgtaattg accgcgtcac ccacaatcgc ggcgatcagc atcagcacta ccatcatatg
    46741 gacgttgaga tcgttggttt ccagtgatgc caacgctccg gcaacaaaca gcaatgaatc
    46801 acccggtaaa aacggcatta ccaccagacc ggtttcacag aacaaaatta agaacaaaat
    46861 ggcataaacc cagacgccgt actccgcgac tagctctgcc agatgcacgt caatgtgcag
    46921 gataaaatcg atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc
    46981 tttcaggctg taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt
    47041 ccgccagaaa tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca
    47101 ccgcgaccag atacagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg
    47161 ccgccagcaa ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc
    47221 tgccgacaat attcctgacc atatgatgta caaaggcatt cgctttgata tcaaccacca
    47281 cataaggacc gtgacgcgtg acattaatat gcataacgtt gcgccacggg gttcgggact
    47341 ggcactgcac cgcacggaac gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc
    47401 gatgcatccg ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg
    47461 ccgggcgcag ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc
    47521 gggcatgaaa atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat
    47581 tcgcatttac gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca
    47641 ccacttgccc ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg
    47701 gttcgttcgc cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat
    47761 tctgccgttg ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat
    47821 aaactggcag ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc
    47881 gatttttact gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagagctg
    47941 gacttgctcc ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc
    48001 cgaagcatcc cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc
    48061 ctgagcaaac gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac
    48121 catctgggca tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc
    48181 ttcgtcctgc aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc
    48241 ttcgctatcc ggcaacaacg gcagcatatt gaacgccagt tgacgaccga agaaatcttc
    48301 ttcgtcaatc ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc
    48361 ttttttgccc tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc
    48421 ctgatcgatt aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc
    48481 gatgacattc cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag
    48541 cggtacgtcg ggttcgagag caaacagtcc gctgctgtcg atcaccaggc aacctgagtt
    48601 ggtcgcttct tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa atgccagctg
    48661 cgcctgcgtc cagtcgaatt cagcggcatc ctgcacggtg attgttttac caccaaagcg
    48721 cagttgttcg cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg
    48781 acgttcagcc agcgtttcaa gcagggcttc gcccatagcg ccagttgcgc ccaggacggc
    48841 aatgttccag ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg
    48901 ttacagcaga gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc
    48961 agtttacaca gcaatgatgc cgcactcgcg tcatcacaaa ttacatacag agatgaccat
    49021 tcacggcgct caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact
    49081 ttacgcagcg gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc
    49141 gtcggttgat cgagcggacc atgcagcgta atgcggccaa actcaggcgc gggcagcaac
    49201 gtatccagcg caacgtgttg ttcatgccca ataaacttgc tataggcttc aaacacttgc
    49261 gtcgtgccac gcgctttacc ctccagggta tagcctgcga tatgcggcgt gccgatatcc
    49321 acttttgtca gcaattccac attaagttcc ggctcgcctt cccagacatc cagcaccacg
    49381 cttaacttct ggccttcatt caggcaggtc agcagcgcgg tattatcgac gactgcgcca
    49441 cggcaggcgt taatcagaat cgctccgggc ttcaggctac ggatcagttt ttcatccgcc
    49501 agatgtagcg ttttgtacgg accatcttta aagagtggcg tatggaaagt cagaatatcc
    49561 gcgcgctgga ctaactcatc cagcgagcgg aaatcaccct catccccacg gtcggcgcga
    49621 ggcggatcgc aaagtaaggt tttaatccct aacgcttcca gtcgcgcctg caatcgacgt
    49681 ccaacgttac caacgcccac gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt
    49741 tcggcaagca tcagcaggga ggagaaaaca tattccacca ccgcaatcgc attacagcca
    49801 ggtgcagcgg aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc
    49861 cccgcagtgg cagttccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact
    49921 ttcgtgaccg aacgcaccat cagcgcatcc gcgtctgcca gttgagcgac ggggattgga
    49981 cgcccgggaa ccgcgatcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata
    50041 ttttcatcaa caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac
    50101 aagtgtgcca taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt
    50161 aatgatacaa cctatttccg cccctcctcc tgggcagcca ccaggtcagg gagataacct
    50221 gccgtctggc gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt
    50281 aatgacgaaa gtgacctcac tgacgcaaca acaaagagca gaactgtggg cgggtatcag
    50341 gcacgatatt ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga
    50401 gcataatctg gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt
    50461 agcgcaatta ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat
    50521 ccgtcataac tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat
    50581 tctcaccctg ttgcaggaag ggaagatggt tattccgcaa ccacagcagc gcgaggcgac
    50641 cgaccgtcct ttattaccgg cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc
    50701 ggcggcaacg ggggaaccca gcaaacagat ctggcaatcg atgctggaac tttccggggt
    50761 gaaagatggc gagttaattc cagcgaaact gtttaaccat ctggtgacct ggctacaggc
    50821 gcgtcagacg ctaagccagc aaaatacgcc gacgctggaa tcactacaga tggcgctaaa
    50881 acaaccttta gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg
    50941 cctttctgcg caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta
    51001 tcaacggcgg gttaaaggga ttgatccgcg tgatatgcaa ccgctgctta atccttttcc
    51061 accgatgatg gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt
    51121 cgcgattatc ctgatgctgg tctggctggt tcgttaaccc cgacgaaatg acagtatcgt
    51181 gacaataata cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa
    51241 cgtggtcgcc agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc
    51301 ggcgtaaccg cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac
    51361 gcccagcgca ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc
    51421 gacccatgca cctggggctt gccagagcag caacaagccc accgtttcta caagcagaga
    51481 gacaatcgcc actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat
    51541 cacgacaaat gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttgct
    51601 ggcaaagtag agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag
    51661 ccctaaccct ggtttccaga taagcccgac aacgctccac agcgatggac gttttcccgc
    51721 cagggccggt actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaataccat
    51781 tgtggtgatc gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggcgc
    51841 accaacagcg agggcaccgt aaatcgccat tccgttccat gacatcactt tgccagagtg
    51901 ttttggccct acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc
    51961 aaacccaaga atcaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg
    52021 caaaatcgcc gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag
    52081 cgccgaacgt tttgcgccat attgatcggc cagtcgcccg gcgtaaccac gcgtcagcac
    52141 cgtagccaga aactgaatcc cgacggcaat gccgaccatg gtattgccat agcccagatc
    52201 atgatgaaca aacagcggga taaccggcaa cggcaaccct acggtcatgt aggtgagaaa
    52261 aaccgcaaaa gcgatgcgga agagcgaaaa actggcagaa gatcgtgttt cggtttggct
    52321 tacagcagtc atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc
    52381 tctctggttt cagggttacg gtgcgttggc aggatttaac gcgtacgtct tttcagaagg
    52441 aaatcgacaa agcgggaagt ttgcctagaa ctggtggcga ttgtcaatga tgtgaaaaag
    52501 ggaaccatca ggttcccttt tgcgttagtg ccggaggccg cattggcgcg taacgtcgga
    52561 tgcgacgctg gcgcgtctta tccgacctac tccggcctac tgcgaattaa tctttcagct
    52621 tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac ataacagtgg
    52681 tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc cgcagcctgc tcgtccagct
    52741 cttcaatgtt gatgctcggg gcgataaagc cgtgttccag catcagcaga gagtagatag
    52801 cttcctgcac acccgcagcg cccagtgagt gaccggtcat ggctttggtt gcagaaatcg
    52861 ccgggctctt atcgccgaac acttcacgga tagctgccag ctctttcacg tcgccaaccg
    52921 gagtcgaagt accgtgagag ttcaggtaat cgattggggt atcaacgcca tgcatcgcca
    52981 tctgcataca acgtactgcg ccttcgccag acggagcaac catgtctgca ccatcagagg
    53041 ttgcgccgta gccaacgatt tcagcataga tgtaagcacc acgcgccagc gcgtgttcca
    53101 gctcttcaac cactaccata ccgccgccgc cagcgataac gaaaccgtca cggtgagcgt
    53161 cgtaagtacg ggaggctttt tccggggtgt cgttgtattt agtagacagc gcacccatcg
    53221 cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc gccgccagca aacacgatgt
    53281 cctgtttgcc cagttggatc tgctctactg cgttaccgat acagtgtgcg gaagtcgcac
    53341 acgcggagct gatggagtag ttaacgccat gaattttaaa cggcgtggcg agacaggcag
    53401 aaacgccgga tgccatcgct ttagtgacca catacgggcc aaccgctttc aggccgcgcg
    53461 ggccgcgcat tgcgtcagcg ccgaacacct ggaaacgcgg ggaaccgccg ccggaacctg
    53521 caatcaggcc aacgcgcggg ttattctggt aagcttccgg agagaggccc gcatcagcga
    53581 ttgcctgctc catagaaagg aatgcataaa tggatgcgtc gctcataaag cgcacaactt
    53641 tgcggtcaat gaggccagtg atatccagtt ttatgttgcc ccagacgtgg ctacgcatgc
    53701 cggaatcctt cagctcctga gagaaagtga tccctgaacg tccttcacgc agagatgcca
    53761 ggacttcctg ctggttatta ccgatgctgg aaacaatgcc caggccagta atcactgcac
    53821 gtttcattca atacctctgt aagtcgcaca tagagtaagt ttcgaatgca caatagcgta
    53881 cacttgtacg ccgaacaagt ccgatcagcc atttaataga gaaatttgcg cagccttaca
    53941 cacatcgcta agatcgagcc accgcctgta agacgagtaa cttacgtgaa acactactcc
    54001 atacaacctg ccaacctcga atttaatgct gagggtacac ctgtttcccg agattttgac
    54061 gatgtctatt tttccaacga taacgggctg gaagagacgc gttatgtttt tctgggaggt
    54121 aaccaattag aggtacgctt tcctgagcat ccacatcctc tgtttgtggt agcagagagc
    54181 ggcttcggca ccggattaaa cttcctgacg ctatggcagg catttgatca gtttcgcgaa
    54241 gcgcatccgc aagcgcaatt acaacgctta catttcatta gttttgagaa atttcccctc
    54301 acccgtgcgg atttagcctt agcgcatcaa cactggccgg aactggctcc gtgggcagaa
    54361 caacttcagg cgcagtggcc aatgcccttg cccggttgcc atcgtttatt gctcgatgaa
    54421 ggccgcgtga cgctggattt atggtttggc gatattaacg aactgaccag ccaactggac
    54481 gattcgctaa atcaaaaagt agatgcctgg tttctggacg gctttgcgcc agctaaaaac
    54541 ccggatatgt ggacacaaaa tctgtttaac gccatggcaa ggttggcgcg tccaggcggc
    54601 acgctggcga catttacgtc tgccggtttt gtccgccgcg gtttgcagga agccggattc
    54661 acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa tgctttgtgg ggtgatggaa
    54721 cagacattac cactcccctg ctccacgccg tggtttaatc gtacgggcag cagcaaacgg
    54781 gaagtagcga ttatcggcgg tggtattgcc agcgcgttgt tgtcgctggc gttattacgg
    54841 cgcggctggc aggtagcgct ttattgcgcg gatgaagccc ccgcgctggg tgcttccggc
    54901 aatcgccagg gggcgctgta tccgttatta agcaaacacg atgaggcgct aaaccgcttt
    54961 ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc aattacccgt taaatttgat
    55021 catgactggt gcggcgtcac gcagttaggc tgggatgaga aaagccagca taaaatcgca
    55081 cagatgttgt caatggattt acccgcagaa ctggctgtag ccgttgaggc aaatgcggtt
    55141 gaacaaatta cgggcgttgc gacaaattgc agcggcatta cttatccgca aggtggttgg
    55201 ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag
    55261 atttattatc aatatcagtt acagaattta tcccgtaagg atgactgttg gttgttgaat
    55321 tttgcaggag atcagcaagc aacacacagc gtagtggtac tggcgaacgg gcatcaaatc
    55381 agccgtttca gccaaacgtc gactctcccg gtgtattcgg ttgccgggca ggtcagccat
    55441 attccgacaa cgccggaact ggcagagctg aagcaggtgc tgtgctatga cgcttatctc
    55501 acgccacaaa atccggcgaa tcaacatcat tgtattggtg ccagttatca tcgcggcagc
    55561 gaagatacgg cgtacagtga ggacgatcag cagcagaatc gccagcggtt gattgattgt
    55621 ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc gcgctgcggt
    55681 gtgcgttgtg ccacccgcga tcatctgcca atggtaggca atgttcccga ttatgaggca
    55741 acactcgtgg aatatgcgtc gttggcggag caaaaagatg aggcggtaag cgcgccggtt
    55801 tttgacgatc tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc tgccccgctg
    55861 tgtgccgaga ttctggcggc gcagatgagc gacgaaccga ttccgatgga tgccagtacg
    55921 ctggcggcgt taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg taaagcggtt
    55981 aaggcgcggt aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc cttatccaac
    56041 ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc atggtgctcc
    56101 agacgccgca acttacagct gtgacgcctg ctgaaacagg ttttcccaca tatcggtcac
    56161 cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg gctttttcca gactctggct
    56221 aacggtcgta tgcaccgcct gagcggagtg gtcatcacca ctttccagtt ctgcgatggc
    56281 taatgtcagg tggccacgca aatacccact ggcaaacaac tcatcatcac tggcatggtc
    56341 aaccatgccg tcgattaatg ccagaatgcg tgattcaaac tccgcgatca tcttctttcc
    56401 tcattgtaaa acgtggcgct ggcttcagtt gagcgcttcc ggccacggga actgttccgc
    56461 cgtaagttcc ggcgtgtgat aataattttg tagtgcttta atgaagcgtg ccgggcgttc
    56521 cggaatgcca tttgtcagat aatccatcac ctgcgcatgt acccgccgtt gaaacaccac
    56581 acgatccggt tcgaaatccc cttccagatt gtcgcaactg acgttaaacg gatatccagc
    56641 cgccacgcag aataaccagt cgaacgcctg cggcttcact tcaacatctt caaactggct
    56701 ttgcgtctgg gcatcgcgtc cgtccgggca ataccagtag ccgaaatcaa ccagctcacg
    56761 gcgcgctttt ccggcaatac accagtgcga aatctcatga attgcgctgg cataaaagcc
    56821 atgagcaaag acgattcggt tatacggtac ttccgcatca gcaggaagat agatcggttc
    56881 gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca aagcagctat taaaaatttc
    56941 aatcaactgc tcgtagtggt gtgtactgtt cattagttca tccccaacca gtggaggatc
    57001 tcctgtccgt ggctatcgta aagtaatttg gcactcatca ccgccgagac gataacaatc
    57061 atcgggcgga tcagcttttg tcctttgctc aacaccaacc gtgaacccat gcgcgcgccg
    57121 aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa tcactttgcc gccgagaata
    57181 aacagcagca gaccgccaat gtttgacgtt gcgttgagta atttggcgtg agccgtggct
    57241 ttggcgaggt tgaatccgca cagcataacg aaggccagcg cgtaaaacga tccggcagcc
    57301 gggccaaaga atccgtcata aaaaccgacg cagccaccgg caattaacgc gaagggtaaa
    57361 ccgtacatcc ggcgctggcg atcttcttca cccagctttg gcatcagcaa gaaatagagg
    57421 ccgatacaaa tcaccagaat gggcaaaatc tgccgtaaga catcagcctg aacgtactgt
    57481 accaacagtg cgccgctcat tgagccgaca aaggtcatgg caatattgag tttctgatcg
    57541 cttaaactaa ccactttgcg gcgaataaag tagatagtgg cggaaataga gccgccgcag
    57601 gcttgcagtt tattggttgc cagcgcgtta gcgggagaca ttcccgcagc catcaatgcc
    57661 ggaatggtga gtaacccacc gccaccggca atcgagtcga taaatcccgc cagcatggcg
    57721 acaaaaaaga gaactcccag caacagcgga gaaaccataa acaggctatt aaacgtttcc
    57781 attagatcac gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct
    57841 tcttctcagg ctttgttgtt cccggttttg gaggttcaaa ccagctttgc agttctgccc
    57901 cgcaaccatc gcctgatggc ggtaaaggtt gatcttcaca ctccagacta tcggcagggc
    57961 aacgtaatcg tacatgcata tgcgcgcgat gctggaacca gggtcgcact ttgcgcaacc
    58021 agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga ttaacaaaaa
    58081 tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa gctgaaaatt tctggcttcc
    58141 acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct tgcgggcgca
    58201 agagctgcgc ggaggtccag cgagttttcg gcagttgcag aaagatatcg acatccagtc
    58261 cggtctggtg gctggcatga ccgccgttga aacgcccacc agcgggcatc cccatatcgc
    58321 caatcagcac cgtacccatg cccagattgc tcacctggct actcagacgc tggataaaca
    58381 tcaccagatc cgggtgaccg aaatagcgac gctgatcggt acgcatgacc tgataatgtt
    58441 cggactgtat cggcaacgtg tcagcgccga caatacagcc attagaaaaa ctgcctatcg
    58501 attgtgcgct acccggcaca ggttgggtta ttttttgcca cggcgtcgct gccaggctag
    58561 cgctactggc aagcagagcc agcagcgcaa tcgcggtttt gttcattttt taccagcgtg
    58621 gaatatcagt cttcacatcg gcattttgcg cccgctgccg taacaggtga tccattaaaa
    58681 cgatcgccag catcgcttca gcgatcggca ctgcacggat cccgacacag ggatcgtgac
    58741 ggcctttggt gatcatctca acttcttcgc caaagcggtt aatagtgcgc cccggcacgg
    58801 taatgctgga ggtcggtttc agcgccatat gagcaatgat ttgctgcccg ctgctgatac
    58861 cgccgaaaat gccgcccgca tggttgctct ggaaaccctc tttggtgatt tcgtcgcggt
    58921 tctggctgcc gcgcagcgcc actacgtcaa aaccgtcgcc aatttccacg cctttcaccg
    58981 cgttgatgct catcagcgca tgggcgatgt cggcatccag gcggtcaaag accggctcgc
    59041 caagtccggc ggggacacca ctggcaacaa cggtgacttt cgcgccgatg gagtcgccct
    59101 cttttttcag cgcgcgcatc agttcatcta acgcgtcgat tttgtcgggg tccgggcaga
    59161 agaacggatt ttgttcaacc agcgaccagt ctttgatttc cagcggaatg tcgcccatct
    59221 gggtcaggca gccgcggatt tcaataccaa atttctcggc gagatatttt ttggcaatcg
    59281 cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga acgtccaccg ccgcgataat
    59341 cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg gcctggacgg aagacgtctt
    59401 taattgcgct gtaatcctga gaacgctggt cggtattttc gatcaacaag ccaatgctgg
    59461 tgccagtagt aacgccttca aaaacaccgg agagtatttt gacctgatcc ggctcgcggc
    59521 gctgggtggt atagcgcgat gtcccagggc gacgacgatc aaggtcatgt tgcaggtccg
    59581 cttccgtcag cggaatgcct ggcggaacac catcgacgat gcagccgagc gccagcccgt
    59641 gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg
    59701 ctccgttatt gttgtgtttg cgtgtttact taatctttat aaatcgcgaa atgttctcgt
    59761 gcggcaagaa gctgctcttt agtgagcata aacacaccat cgccgccgtt atcaaactcc
    59821 agccaggtga acggaacatc gggatattgt tccataagat gtaccatgct gttgcccact
    59881 tcacaaatca acacgccatc atcagcaagg taatctgccg cgttaccgag aatgcgacgc
    59941 gtcagtttca ggccgtcggt gccagatgcc aggcccagtt ccggctcgtg acggtattcg
    60001 tttggcaggt cggacatatc ttccgcgtcg acatacggcg ggttagtgac aatcagatcg
    60061 tattgcactt tcggcaaatc acggaacaga tcggaacgaa tcggaatgac gttgtggatc
    60121 agaccgtgtt cttcgatgtt ctgttcggca accgccagcg cgtcgggaga gatatctacc
    60181 gcgtcgactt ccgcttccgg gaaggcataa gcacaggcaa tggcgatgca gccgctaccg
    60241 gtacacatat ctaaaatatg ctgcggttgc ttgctgatga gtccggcaaa tttattgttg
    60301 atcagttcac caatcggcga gcgcggcacc agcacgcgtt catcgacgta aaattcatga
    60361 ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa tgcgctcgtt gacgcggcgg
    60421 atcacgcgtt caacaatacg gtgtttttcg ctggaggtca gacgcgcggt gtgcatatct
    60481 tccgggatat ccagcggcag gtagagcgat ggtaacacca gctgtacggc ttcatcccac
    60541 gggttatcgg tgccgtgacc gtaccagata tttgccgcgc tgaagcggct taccgaccag
    60601 cgcaacatgt cctgaatggt ttgcagctca tttactgctt catcaacgaa aattttatcc
    60661 acgtattcct ccagggcatg atcgcaataa tttcggcggc tagtttgcca tgaagatgac
    60721 gataaatcag cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg atcgattgtg
    60781 ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga cagataaatg aaaaagaaaa
    60841 caacacttag cgaggaggac caggctctgt ttcgccagtt gatggcgggg actcgcaaga
    60901 ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa aatcagcgaa gtgccggtga
    60961 aacgcttgat ccaggagcag gctgatgcca gccattattt ctccgatgag tttcagccgt
    61021 tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga tgtcagccat tttgaggcga
    61081 agaaactgcg ccgcggcgat tattcaccgg aattgttttt ggatttacac ggtctgacgc
    61141 agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc ctgtcgccgc gaacatgtgt
    61201 tttgtgcctg cgtgatgcat gggcacggga agcatatttt gaagcaacaa acaccgctgt
    61261 ggctggcaca acatccgcat gtgatggcat ttcaccaggc accgaaagag tacggcggtg
    61321 atgcggcttt gttggtgttg attgaagtgg aagagtggtt gccgccggag ttgccctgaa
    61381 tgctggcagc aaatgccgga gcagcgtaaa cgccttatcc ggcctacaaa tatgtgataa
    61441 ttcaataaat tgcaattcac ttcgtaggcc tgataagacg catcagagca atactacgaa
    61501 cttcagttca ttcaatacat actgaaaacg acacgtcgaa tcaatcggtt gcccactact
    61561 ggtattcaca ttcaccacaa tatcgtgtag tactttatcc gttaccggat gacgagttgg
    61621 cgtaaccgtt accgttgatt tatcacaacc gtaagaggta aaggttaaca attctgatac
    61681 cgcgcggcag tatgctcctc tgccatcatt atcagaaaaa ttactgccat cacagagtct
    61741 gccgccttcc atcaggtagg tcgaaagcgt ggtggtcact gttgaccccg tgaactgaat
    61801 tcgtaacgcc ttattatggc tgacataatt ttcactccag gagatatcgt atgaagcgac
    61861 cggtatcgtt gggcatattg tgttatgcac tgtaggggcc gtatagcttc caaatccggg
    61921 taaatctgta taggaataaa aggatgcgat ccaagggcaa ccataatcat cagatgcaac
    61981 ggtaatttta aacgtcatat atgcacaacg aaaaattttc gtatctatgc cagggcaatt
    62041 tgagttatta attgtatcaa agtcagtcag taccaaagtt ggagctgaac ccgacccaga
    62101 ccaggaaata acccgcaacg tatcgccttt atacgcaaaa gaatcatatt tcccatatgc
    62161 aacctccttt ggcgttatca ccgtaacatt gaaaacactg ctcgctacag caccggttac
    62221 cttgtccatg gcataataaa ctctcacact gtcaaaactc gcactcataa gtttatctcc
    62281 agcataactc ttagatgcgc cgacaacact cagaacgagc agcattaggc tcgcgaaaat
    62341 tatggtccat tttttcattt cagaaatact caaaggttgc cgtcactgtg gcactaaagt
    62401 ctccggacgt tacatctcgc ccgctctttg cctgtaacca ggtattaaaa tttaggctgt
    62461 tatttccctg attcagcggg agcgtgacgc ctgttgtatt gttgattggc accgatgtgc
    62521 ctgccgcagt ttcaatacca atcccagcgc cggatgctga tgacgaggag tcaaaggcca
    62581 gaaagccggg tagagcacta tcttctgttc ctgtcaacgt tactttgaca ttaaacaatg
    62641 tagaactatg acaatctttc aattgaaaca ccaatggcac tcgctctgac tgcccaaggt
    62701 tcattaaatc gctggcggca atggcaggaa aatgtacttc tgccaactgg ctaccattaa
    62761 tcactggcgt acacgattta ctgattaaat tgccggtaaa gtgcaaatta tctcctgcta
    62821 ttgtggttcc actgcccagc aacatcaggc tactaataaa aaacaacgta cgctttttct
    62881 tcattggtaa tctacctgta acgtggccca ggcctcaaag tccgcctctg gcagaatgac
    62941 accactcttt tttactggca cagccgttaa caccggtaaa tcggtttcat tcaccacaag
    63001 tggcgtattg atcgtaaaac tctgccctgc ctgtttgagc tgaatgccta aaccagtgat
    63061 attgctgctt actgcagcaa tatcaaaatc gcttggtgtg ccactccagc tcaaggtcat
    63121 ttccattaat gggtcgcgaa ccgccgtatc gcaggtaatg gaatacggca cggtttgctc
    63181 ataattgctg ccattaatgt catcaataag cacatcctga aaagttattt caatggtttg
    63241 agcgttgcta atcgtgcagt taggcggctg cactaaataa ccgtgaaaag agacgtttga
    63301 gagtgccatc gagcaaaagc cccacagcaa aataagcgag atccgtttca ttggtaatcc
    63361 accacaattg tggcactggc gttaaattca gctgccgcta acgttgtacc actctgtttc
    63421 accggcacag cttccagaac gggaactccc gaggaaaaat taaatggcaa ccatgagcca
    63481 gaggttaagt cgagtattgt gtgatcacta cttttctgaa cccgaatacc aaacccagga
    63541 atacccgtgc tgataactgt ttcaccgtta atcacgaccg ttgtcgcctg catctgtaga
    63601 cgcaggtcat ccattgccag attgttacaa acaagcgagt agtcaatggg acgtttataa
    63661 tcaaccctat tgatcttatt aatgaataca ttaccaaact caactgccgc accatttacc
    63721 gtacaaggag gaggggcatc gacaagcacc cgtaaattca tacttactga cttactcgct
    63781 gcaactgcaa agccgctacc aacacaacat atcagtaacc cagttaccgc ccagacagaa
    63841 caagatcctt tcatactggc tctcccggcc tcagcctttg cggctctgct cttcatcgac
    63901 ctagcaactg ttaccgttgc aagtgaaaaa gagcggcatc cgagcgccat agtcgttgac
    63961 ataggtcagt accggcacgc tacccatctt cacattcagc ggtgcatagg ttttcggcga
    64021 catcactatt ggcgagaaac cggctgccgg attgccattt ttctgtgtgc tggcattact
    64081 gaaaatgacg taatacgacg tcgggttatt gacggtaaat tcctgaccgt tgcgggttaa
    64141 agtaacctta tattgctagg ggtttttcat gctgacgttc tccagctcct tcggtcgcca
    64201 gaataactta atacgcgttt gtagtgctat ttgcagcgtg ttggctttgt ttgattttgg
    64261 cgggatctca cgcacgttga agtagaacag gctttcacga tcggcaggca atttgttgat
    64321 atccggcatt ccctgcactt ttacctgccc gttcatcatt gaatcgatgc gttgcaccgg
    64381 tggcagaaca gttaacggtg aactgatctt gttacccttt tcatcttcga tccagctttg
    64441 ggccagataa ggcagtttcg gatcgttatt gcgcaacgtc acgctgattg atttgtcgct
    64501 ttcattaaat atcaaacggg tacgatctgg cgttacagaa gcctgggcag acaaactggc
    64561 agcgctaagc agtaacgcta gcgtcgtagc acccaatttg gcagagcaaa gtaaatcaga
    64621 catggatcat tccttaatca cgttattggt tagctgaaac agaaacaggg ggattggtgg
    64681 gtgtcacctg ttgcgtctgc tcctgaatga caggctttat gtcatcagga acaaccggcg
    64741 cgacgttgcc tttatgctgg catggcagta acaagccgtt aaacagatcg gcaggcagcg
    64801 ggtccggcag gttgatatcg caatgcgcaa caccactcca gaacacactc atgtgttcgc
    64861 caggtttcac cccagccaga taaacattgc cgtcatcatc gacaaggccc actgtctgct
    64921 cgttatcatt ttttacttct gcgccaaacg gaggatggct gccgtcttgt aaacgcagca
    64981 ccgccatcgc tttttgacca ctgatgacgg caaatttgcg gtagccaatc gccccttcag
    65041 ttagcgtggc ttgtaccacc gactgggttg cttcggcgtt ttccggcagt ttgttgaggt
    65101 cgatatacgc ctgattgcgg taatagttat tgacgtcaga aacgacggct ttaccaaaca
    65161 tattggtata aacagcagca ccgttacctt ctaccggaac atcggcaacg ccatcggcat
    65221 caatcaacaa gcgtgtcccg cccatattct gggtacggtg aagtgcgcca ccgtgggcag
    65281 taagcgttgc gccgccctgt aacgacaagc ccgcagaggt gtattgccct tcatggtagt
    65341 tcgcactgag gtcaacctgc gccagcgaac catcatggct gtaatagccg tcaacgctgg
    65401 tgtgtttgtc actggtgccg acgttcaact gatagtgagt cgcgtcatcg acacggctga
    65461 aataaccgac ctgactgctg tccgtcccac tgccatagta ccgttatagc taacggtact
    65521 gttgtcgccc cacggcatac tgagcgaaat gtacatgcct ttgtcggcct ggttgtcata
    65581 ctcgtaacgg tagccagtca tcgagatgct gacattacga atactgccca tattgaaata
    65641 gtgcgagagc atgatgttgt agtttgtctg ctcctcgcga tcccagtagg tatggcgggt
    65701 atagttgaga taaaccgaaa cacccgcatc gcggaagttc tggttataag tggcggtgta
    65761 catctctttg tcgttgcccg tgcgtaccat tccgctgtca ctggcatcca gatactcgct
    65821 catggtcata aagttctctt ccgagaagcg atatccagca aaagtaacgc ggctgttgag
    65881 ctggtcaaag tctttggaat aactcacacg gaaggagtta ccgtccagcg aacctttgcc
    65941 ataagcggtg tctttatcca gtttggtgtg cgagtgagta acatcaaacg cgaccgcgcc
    66001 gaatgtagac aaatcgcgac cgacaccaag cgccgcagac tgatagtttt catctcccag
    66061 tgcgccgcca tataacgacc agccgttagc gatcccccag gaagcttcag caccagaaaa
    66121 aaattcaccc tcgacatggt gtccccactc ttgcggacgg cccatcatga tcttatagcg
    66181 aacctgacct ggacgagtga ggtatggcat cgaggcggtg ctgatgtcat attcctgcac
    66241 ctggccgttc tgttcttcaa tgcgaatatg caacgtaccg gagacagaat caccaagatc
    66301 ctgaatacga aacggtccag ccggaacctg cgtttcgtaa atcacacgcc ccatctggct
    66361 gacggtcact tttgctgtgg tgtgtgccac gccggaaatg tctggcgcgt agccgcgcag
    66421 attgggaggc aacatttgat cgtcagtact gacactgcca ccaacatagt taaaaccatc
    66481 aaaaatatcg gatctgaggt aatcctcgcc cagcgccagt ttggctttta atgatggtaa
    66541 cgcccgcctg gcatagtagc gactccactc ccattttttt tgagtttcat cgccgctgaa
    66601 ttcatcgtca tcattactgc gagtatgttg atagttagtc tgccagtcag cacgcatacg
    66661 ccacggccct aggttaaccc caaccgtccc gttgccgctg atctcgttac tatcatcacc
    66721 gccattttct tcgtgccggg tttgtgcgtt gatgctgtag tccgcgacga tcccggagat
    66781 gccgtcatcc caacgtgaag gcggatccca gtcgggataa gtatattcga ggtaagcctg
    66841 tggcagtgaa atgactaatg cggactggct taaatcagcc ttaatttcca tgccttccag
    66901 ttgaccggat ttcaggcatt tagcatcgtg gctccattgc agatttttcg ccacgtcttc
    66961 ttttaaacca aactgcgcta ccagttccgg tgtcagacaa gcatagcttt tgctcgcgtc
    67021 atcttcacca gcataccagt aaatatcgta ctcttccgcc aatggctgtt tatttagttg
    67081 aacctgtaaa ttgtattttc cgggctcaac atatccctgg ctggaaaaac gcttcagatc
    67141 aatttttgtg tcgcctttta attccagaaa acgggaatca aactgaatgt cgtcttcagc
    67201 ccagacactg ctataactcc ctgacattgc cagcgcaatg caccacggaa gaatccgtaa
    67261 tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt ttttattctt gttcggtcaa
    67321 aatccattta aatcccccat agcagtgtcg ctatgggggt atgccttaca ggtaagtaat
    67381 ctggaaggtg gtatttgctt caaaattacc cagatctggc gcatcagctg cgccgaccag
    67441 ccaggcttta aagttcagtg tctgcttcgc tttgcctttg ttggtagaag tgtctttcac
    67501 tattttctgg tcaataccca tgccgctttt gtaagaggta cccagagagt caccgatcgc
    67561 caggctaaca ttgttaaatg ccgcaccggt atcggtattg aaaatggtgt aataattgcc
    67621 gctatttgca gaagaaacgg taccagtaaa ggtagtggtc attgtttcct gagtatcaaa
    67681 tacgcaatct tgcagacgaa tctggaaact tttcggcgtg gtggtgccgt tatttttcag
    67741 tttttcggca ccgatatcac ccatatcaac ttccagtttg tgatcgtccg gaacgataga
    67801 acacactgag tcttcaatgg tgccgtaaaa acgtgcagta ccgttgttac ccgcagcgat
    67861 tgttgccgta gaggtgaaca cgcccatcac cattgccgca gcaatagctg ttttaacaaa
    67921 cttactcatt atttgtcctt aaactataaa taattaaaaa tataaacatt gcaatacatt
    67981 gattcagtca atagccaatg tttataccca gccattgaat acggcatgcg acccaaaaag
    68041 aacaggtcgt agaaaattgt attcattggt gtggggccgc atagtgatgt ttccacaccc
    68101 ctgttccaat ttaattttac taaagagtaa atgtagattt aattaatata ttgacagaag
    68161 aaaggttatc ttaaggacat ccttaaaaaa tacggaataa gtatttagaa aaaatagaaa
    68221 gtatatcaat aatgaatatc aacagcttaa aaaacaaaca atgaacatat aatgcgattg
    68281 gcatttatcc acttttgaca atataatata taaacactat agccagaatt taatcaagtt
    68341 tcaattacat cctgcctgat tattcaactc tattttctct attatctgta ttaataaatg
    68401 acactgagtg tcaaacagac attattcatt tcaacatgta actttgggca aaatcaagat
    68461 acgcgcagaa gaataatttc cgaatgaatg tagaaagtgt tttatttata gataaggaga
    68521 gtcttcgata gtagtgaggt tgacagatat ccgctgccta atgagcaatt atgcccaatg
    68581 aatctacctc attgggcata atttggaaca catcagatag cttttgccat cttcagatta
    68641 cacgggctca tctgccagtt gaatgtaccg tttccgcttt catcaagcgt tacgctggca
    68701 atggccgagg tggtaaacat aggcggcgtt tcgcccggac ataactcggc aaccagatac
    68761 ccgactaacg gtaagtgaga gatcaccagt accgaagcaa caccttcatt ggtcaacgcc
    68821 tgcaaatagg cactgaccag accaacatcg ccgcagggcg ttaactccgg cagaacttct
    68881 gcactggaag gcaggttcag acaatcccct acttcttcca gtgtttgctc ggctcgcagg
    68941 aacgggctca ccagaacgcg ttcgatttcc actttttgac ctttcagcca gttcgccatc
    69001 aggcgagatt cgtcacaacc attagtggtc agaggacgaa ccgaatcact ggcggcatcg
    69061 agggctgcgt cgccgtgacg catgataaaa acttgcatat tgcaccgctt ttgttaacca
    69121 gattcgcccg ttttctttca tctgaaacca gagaaagaaa acggtggccg gcattgtgcc
    69181 ttatccattc accgaatgaa acgctgtttt ttacctcaat ggcgtaagta tagtcaatat
    69241 gcgtttacat tttgaccaac actccgccat tcagcgcgga ttcatatagc tttgaccttc
    69301 ttattgcagg tcagttgcag ttgttttcca aaaactttcc ccacgcgcgc ccatctcgac
    69361 caaacgctcg caaggggtaa aacgggaacc atactgcgtg gccagtcgtt gcattattgc
    69421 aaccacttcg cccgcgccga gagaatcgat atagcggaac ggtccaccga gaaatggcgg
    69481 aaaaccaatg ccaaataccg caccaatatc cccgtcacgc acgctacgga taacctgctc
    69541 atcaacacaa cgtactgctt cattcagcat caacatcaca caccgttcag caacctgcgg
    69601 tgcggagagt cgcccctgcc cttgtgcgcc aatgagcggg taaatggcag gatcgacctg
    69661 ttttttgctt ttacgccctt tctgaccata aagatagaaa ccccgaccat tttttctgcc
    69721 tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca ggcgcgctaa aacgttctcc
    69781 ataagcggct tccagtacag gaataatttt agtcccggtg tcgattccta cctcatccaa
    69841 aagttggatt gggcctaccg gaaaaccaaa tttcactagc gcggcatcaa tgtgctctac
    69901 ccgttcacct tcggtcagca tgcggatagc ttcattaatg taaggcgcta agatgcgatt
    69961 gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt ttgccctgtt ttttcgccag
    70021 ttttaccgta gtggcgatgg tttgcgccga tgtacccgca tgaggaataa tctctaccag
    70081 cggcattttt tccaccggac tgaagaaatg caggccaata acttgctcag gtcgcgcggc
    70141 gtgagcggcg atatcaccaa tcggtaaaga tgacgtgttc gaagcaaaaa tggtatgagc
    70201 ggcgcaattt tgctccactt ccgccaccat ctgttgtttt aattcgagat tttcaaacac
    70261 cgcttcaata atcagatcgc gatgggcaaa gccgcgatag tccgtcgttc cggagattaa
    70321 tgccagctgt ttgtcacgtt cgctggcttt gagatgacga cggcgaactt tgccctccag
    70381 ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc gggttgatat ctttaattct
    70441 gaccggaagc cccgctttac aagcagtgac ataagcaata ccgccgccca tcaagccacc
    70501 acctaaaatt cccacgctgt ttaatggcgc aggcggtgca tcactaccgg gatctttctt
    70561 cacgtccgta ctggcaaaaa agatactacg caaagcttgc gattgcggcg tcatcgccag
    70621 ttcgccaaag gcgcgagctt cagcgtcata gccgctgctg gtgccctgcg ctaatcccgt
    70681 ttcaacaacc tccaggatgc gttctgtcgc cggataattg ccttgagttt tgtgttctgt
    70741 tttcttgccg accattttga acagcagcgc acgacctaac ggccccgcca gaatacgctc
    70801 gcgtacaggc agagggcggg aagatgggcg atccttcttc gccagctcaa cagcggcttc
    70861 cagcaaaatg gagtgcggaa caacgtcatc caccagcccc agctttaatg cctgtttcgc
    70921 ccgaagttgt tttccggtga gaatcatctc taatgctgta ctgacgccta tcagacgcgg
    70981 taaacgctgg gtgccgcctg aaccgggtaa caatccaagt tgtacttcag gcaaaccgag
    71041 caccgtttta ggatcgtcag tacaaacgcg accgtggcac gccagcgcca gctccagccc
    71101 accgcccagg caagcgccat gaatagccgc gataaccgga atgggcaaag cattaatctc
    71161 cgccatcaac tgttggccct gccgcgccag tgcttccgct tcttgcgccg ttttgcagtt
    71221 gccaatcatg ttgatgtctg ctccggcgat aaaattgtcc ggtttcgcgg agataaacac
    71281 cacgccgcgc aactctttgt tttcacggag ttgcttaata atggcgcgca cctgcgaggc
    71341 aaattccgcc ttcagggtat tcattttctc atccggtacg tcgatggtga tgatagcaat
    71401 gttgtccaga cgaacattaa gagtaaacgc tgatgccatt tccattattc cgcctccaga
    71461 accattgccg cgccaagccc accggcagca caggcggtaa ctaaaccaaa tccaccgccg
    71521 cgacggcgaa gttcatgcaa tgtctgggta atcatccgcg cgccggtcgc cgcgaaggga
    71581 tgtccgtaag caatcgaacc gccaagcacg ttaaatttgc tatcgtccac ttcgccagtg
    71641 gcatgtgcac gccccagtac ttcacgagca aaacgttcac tacccagcaa ctgaatattc
    71701 gccagtgtct gagctgcaaa ggcttcgtgc atatcgatca atgtcagttc gctcatcgtc
    71761 aaaccggcac gttccagcgc cagcggtgtt gaccaggctg gaccgagcaa catgtcctgc
    71821 cagacatcaa tcgcagtaaa tgcgtagctg cgcagatacc ccagcggcac cagccctaat
    71881 tctttcgccc gggattcagt catcaggatc accgctgccg cgccatcggt cagcggcgta
    71941 ctgtttgccg ccgttaccgt tccgtgtttg cgatcaaacg ccgggcgcag ctttgcgtaa
    72001 tcggcaagcg aggaattacc gcgaatattg ttgtcttcga caagcggttg tttataagga
    72061 gggataaagg cagtcatcac ctcttctttg agttttccgt ctgaccatgc ctgagcggca
    72121 cgctgatgcg aacggtgcgc taatgcatct tgctgttctc gggtgatgcc gtaggttttc
    72181 gccatttgtt cggcggtgtc gcccatccgc aagccagtag aatattctgc taccgcaggc
    72241 ggtacgggca ttaagtcgcg caaacgcagg cgagagaaga gtttcaggcg ctggctcatg
    72301 gttcgagctt tgttgacatc aaccagcacg tgcgccagtt ttttactgac gccaattggc
    72361 aatacggaag aggaatctgc cccaccggca atccccgctc ggatagttcc cgccatcagg
    72421 ctttctgcga cgtttgcaac cgcctggaaa ctggtagcgc aagcgcggct gacgctgtaa
    72481 gcatcggtat gcacattcat tccggtaccg agaacaattt cacgcgcaat gttgggggct
    72541 tcaggcattt gtacgacctg accaaagacc agctgttcaa tcacttcagc ggggatctcg
    72601 ctgcgtgcca gcagttcgcc taccaccatc ttccctaaat caaccgcggg aatgccatga
    72661 aaagccgtcg cctggcgggc aaaaggcgta cgtaaaccgc taacaatggc gatacgatcg
    72721 ccctggcggg taaccagtgg taaaacctga cccataacac tcccctgtaa aaaaataaat
    72781 aaagtggtct gacctgatca tagtattaac cattttttta catttagcca agcggagaaa
    72841 aaggaaagtg ggagctatga cacagtgaag acagaagaaa gaaaagaaaa cgcccctgcc
    72901 agcaactgac aggggcgtac gtacccgaga ggaattaacg cagacccagc tggaagatca
    72961 gcatttcagc ttcgcaggca aaagtgaaat cgatatccag gcgcacaccg tcagactctt
    73021 cagtgaaagt cggggagatt ttgcacggtt cggattccac gctacgggat ttttcagtca
    73081 gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa cacgcggctg taagacgcgg
    73141 tgcagtcgga gttgtccata atggtgccaa catccataca gcagcaaacc ggggtttcat
    73201 cagcactaca tttactcatc gttgatttcc tctgtatgtg cacccaaggt gccagataaa
    73261 cgttgtggat attttacgct tccggaaagt gctgctccag ttgttaattc tgcaaaatcg
    73321 gataagtgac cgaaatcaca cttaaaaatg atctaaaaca aaattcaccc gaatccgcga
    73381 gtgcgcaacc tccaaatttt gccagctgga tcgcgtttct tagatcatat ttgaaaaaag
    73441 atggaaacat acttgcaaca ttctagctgg tccgacctat actctcgcca ctggtctgat
    73501 ttctaagatg tacctcagac cctacacttc gcgctcctgt tacagcacgt aacatagttt
    73561 gtataaaaat aaatcattga ggttatggtc atgagccaga aaaccctgtt tacaaagtct
    73621 gctctcgcag tcgcagtggc acttatctcc acccaggcct ggtcggcagg ctttcagtta
    73681 aacgaatttt cttcctctgg cctgggccgg gcttattcag gggaaggcgc aattgccgat
    73741 gatgcaggta acgttagccg taaccccgca ttgattacca tgtttgaccg cccgacattt
    73801 tctgcgggtg cggtttatat tgacccggat gtgaatatca gcggaacgtc tccgtctggt
    73861 cgtagcctgc aagccgataa catcgcgcct acggcatggg ttccgaacat gcactttgtt
    73921 gcaccgatta acgaccaatt tggttggggc gcttctatta cctctaacta tggtctggca
    73981 acagagttta atgatactta tgcaggcggc tctgtcgggg gtacaactga ccttgaaacc
    74041 atgaacctga acttaagcgg tgcgtatcgc ttaaataatg catggagctt tggtcttggt
    74101 ttcaacgcca tctacgctcg cgcgaaaatt gaacgtttcg caggcgatct ggggcagttg
    74161 gttgctggtc agattatgca atctcctgcc gggcaaactc ctcaagggca agcattggca
    74221 gctaccgcca acggtatcga cagtaatacc aaaatcgctc atctgaacgg taaccagtgg
    74281 ggctttggct ggaacgccgg aatcctgtat gaactggata aaaataaccg ctatgcactg
    74341 acctaccgtt ctgaagtgaa aattgacttc aaaggtaact acagcagcga tcttaatcgt
    74401 gcgtttaata actacggttt gccaattcct accgcgacag gtggcgcaac gcaatcgggt
    74461 tatctgacgc tgaacctgcc tgaaatgtgg gaagtgtcag gttataaccg tgttgatcca
    74521 cagtgggcga ttcactatag cctggcttac accagctgga gtcagttcca gcagctgaaa
    74581 gcgacctcaa ccagtggcga cacgctgttc cagaaacatg aaggctttaa agatgcttac
    74641 cgcatcgcgt tgggtaccac ttattactac gatgataact ggaccttccg taccggtatc
    74701 gcctttgatg acagcccagt tccggctcag aatcgttcta tctccattcc ggaccaggac
    74761 cgtttctggc tgagtgcagg tacgacttac gcatttaata aagatgcttc agtcgacgtt
    74821 ggtgtttctt atatgcacgg tcagagcgtg aaaattaacg aaggcccata ccagttcgag
    74881 tctgaaggta aagcctggct gttcggtact aactttaact acgcgttctg ataacgcgtt
    74941 cgcctggata aagtcacctg ctatgcaggt gactttaact cccccacttc accgaaagta
    75001 gtttcctcat tgcttgcctc ctgagttttg caaacagcct gatggcagct tgccctttca
    75061 aatcagtaag tggtatccac agacaaacct gaagggaaat gcatattttc aagcgttctg
    75121 ctcgtccttc tcaaagagtt tacttttctg catttccagg atactcaacc cccctggcta
    75181 ttgtgcgctt atacactcaa attaaagata ggttctaaat aaatgagcgt tttttgatag
    75241 tctatttcat taggtaatat atatttgtaa caaatcaatc aacatagaat aaaattatgc
    75301 taccatctat ttcaatcaac aataccagcg cagcttaccc agaatccatc aatgaaaata
    75361 acaatgatga aattaatgga ttagtacaag agttcaaaaa cctttttaat ggtaaggaag
    75421 gaataagcac ctgtattaaa catctacttg agcttataaa aaacgccata cgagtaaacg
    75481 acgatcctta tagatttaat attaataatt cctcagttac ttatattgat attggctcca
    75541 atgatacaga ccatattact attggtatcg acaaccaaga accaatagaa ttacctgcga
    75601 actataaaga caaagaactc gtccgtacta tcattaatga caacatagtt gagaagactc
    75661 atgatatcaa taacaaggaa atgatcttca gcgcattaaa agaaatatat gatggagatc
    75721 ctggttttat cttcgataag atatcacaca aactcagaca tacggtaacg gaatttgatg
    75781 agagcgggaa aagcgaacca acggacttat ttacctggta cggtaaagat aaaaaaggcg
    75841 actctctcgc tattgtaatt aaaaataaaa acggaaatga ttacttatct ctcggttact
    75901 acgatcagga cgactaccac attcaaagag gaattcgtat taatggtgat agtctcaccc
    75961 aatattgtag tgaaaacgcc aggagtgctt cagcgtggtt tgaaagcagt aaagctatca
    76021 tggcagaatc atttgcaact ggttccgatc atcaggttgt aaacgagctc aacggggaaa
    76081 gactgagaga acctaacgaa gtttttaaac gtttaggtcg agcaataaga tataattttc
    76141 aagtggacga tgcaaaattt agacgcgata atgtaaaaga aataatttct actttattcg
    76201 ctaacaaagt tgacgttgac catcctgaaa ataaatacaa agactttaag gatctcgaag
    76261 ataaagttga aaaaagactt caaaattgcc aggcgaaata tcaaaatgaa attaatcaac
    76321 tatctgcact aggtgttaat tttgatgata tttaaaatta tttttccagt tttttgattt
    76381 ttcaacgcct gaatatcgga aatagtcagt gacaaac
//
LOCUS       DADMFL010000004        69006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.004, whole genome shotgun sequence.
ACCESSION   DADMFL010000004 DADMFL010000000
VERSION     DADMFL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.004"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..188
                     /locus_tag="JNZ27_000249"
     CDS             <1..188
                     /locus_tag="JNZ27_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8662728.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNZ27_000250"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNZ27_000250"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY8662729.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /locus_tag="JNZ27_000251"
     CDS             1156..3192
                     /locus_tag="JNZ27_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY8662730.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="JNZ27_000252"
     CDS             3150..3545
                     /locus_tag="JNZ27_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY8662731.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="JNZ27_000253"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="JNZ27_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY8662732.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="JNZ27_000254"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="JNZ27_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY8662733.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="JNZ27_000255"
     CDS             complement(4854..5894)
                     /locus_tag="JNZ27_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8662734.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="JNZ27_000256"
     CDS             complement(5967..6602)
                     /locus_tag="JNZ27_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY8662735.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="JNZ27_000257"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="JNZ27_000257"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY8662736.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTNRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="JNZ27_000258"
     CDS             complement(7228..7671)
                     /locus_tag="JNZ27_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY8662737.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNZ27_000259"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNZ27_000259"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY8662738.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="JNZ27_000260"
     CDS             complement(8011..8514)
                     /locus_tag="JNZ27_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8662739.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRRQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="JNZ27_000261"
     CDS             complement(8508..9032)
                     /locus_tag="JNZ27_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY8662740.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="JNZ27_000262"
     CDS             9241..10236
                     /locus_tag="JNZ27_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8662741.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="JNZ27_000263"
     CDS             10245..11123
                     /locus_tag="JNZ27_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8662742.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="JNZ27_000264"
     CDS             11204..12211
                     /locus_tag="JNZ27_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY8662743.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNZ27_000265"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="JNZ27_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY8662744.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /locus_tag="JNZ27_000266"
     CDS             complement(13727..15616)
                     /locus_tag="JNZ27_000266"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY8662745.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNZ27_000267"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="JNZ27_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY8662746.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNZ27_000268"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="JNZ27_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY8662747.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNZ27_000269"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="JNZ27_000269"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY8662748.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNZ27_000270"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="JNZ27_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY8662749.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNZ27_000271"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="JNZ27_000271"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY8662750.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNZ27_000272"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="JNZ27_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY8662751.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNZ27_000273"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="JNZ27_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY8662752.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNZ27_000274"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="JNZ27_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY8662753.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNZ27_000275"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="JNZ27_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY8662754.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="JNZ27_000276"
     tRNA            complement(25945..26021)
                     /locus_tag="JNZ27_000276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="JNZ27_000277"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="JNZ27_000277"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY8662755.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29327)
                     /locus_tag="JNZ27_000278"
                     /pseudo
     CDS             complement(27720..29327)
                     /locus_tag="JNZ27_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29805..29891)
                     /locus_tag="JNZ27_000279"
     tRNA            complement(29805..29891)
                     /locus_tag="JNZ27_000279"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29855..29857),aa:Leu,seq:gag)
     gene            complement(29906..30238)
                     /gene="secG"
                     /locus_tag="JNZ27_000280"
     CDS             complement(29906..30238)
                     /gene="secG"
                     /locus_tag="JNZ27_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY8662756.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="JNZ27_000281"
     CDS             complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="JNZ27_000281"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY8662757.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31796..32644)
                     /gene="folP"
                     /locus_tag="JNZ27_000282"
     CDS             complement(31796..32644)
                     /gene="folP"
                     /locus_tag="JNZ27_000282"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY8662758.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="JNZ27_000283"
     CDS             complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="JNZ27_000283"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY8662759.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="JNZ27_000284"
     CDS             complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="JNZ27_000284"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY8662760.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35523..35816
                     /gene="yhbY"
                     /locus_tag="JNZ27_000285"
     CDS             35523..35816
                     /gene="yhbY"
                     /locus_tag="JNZ27_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY8662761.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35972..36448)
                     /gene="greA"
                     /locus_tag="JNZ27_000286"
     CDS             complement(35972..36448)
                     /gene="greA"
                     /locus_tag="JNZ27_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY8662762.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36696..38129
                     /gene="dacB"
                     /locus_tag="JNZ27_000287"
     CDS             36696..38129
                     /gene="dacB"
                     /locus_tag="JNZ27_000287"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY8662763.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="JNZ27_000288"
     CDS             complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="JNZ27_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY8662764.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39503..40468)
                     /locus_tag="JNZ27_000289"
     CDS             complement(39503..40468)
                     /locus_tag="JNZ27_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8662765.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="JNZ27_000290"
     CDS             complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="JNZ27_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY8662766.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="JNZ27_000291"
     CDS             complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="JNZ27_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY8662767.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41443..42414
                     /gene="ispB"
                     /locus_tag="JNZ27_000292"
     CDS             41443..42414
                     /gene="ispB"
                     /locus_tag="JNZ27_000292"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY8662768.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42642..42920
                     /gene="sfsB"
                     /locus_tag="JNZ27_000293"
     CDS             42642..42920
                     /gene="sfsB"
                     /locus_tag="JNZ27_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY8662769.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42968..44227)
                     /gene="murA"
                     /locus_tag="JNZ27_000294"
     CDS             complement(42968..44227)
                     /gene="murA"
                     /locus_tag="JNZ27_000294"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY8662770.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="JNZ27_000295"
     CDS             complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="JNZ27_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY8662771.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="JNZ27_000296"
     CDS             complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="JNZ27_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY8662772.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="JNZ27_000297"
     CDS             complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="JNZ27_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY8662773.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="JNZ27_000298"
     CDS             complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="JNZ27_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY8662774.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="JNZ27_000299"
     CDS             complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="JNZ27_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY8662775.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="JNZ27_000300"
     CDS             complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="JNZ27_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY8662776.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48008..48973
                     /locus_tag="JNZ27_000301"
     CDS             48008..48973
                     /locus_tag="JNZ27_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY8662777.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48987..49973
                     /gene="kdsD"
                     /locus_tag="JNZ27_000302"
     CDS             48987..49973
                     /gene="kdsD"
                     /locus_tag="JNZ27_000302"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY8662778.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILTVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49994..50560
                     /gene="kdsC"
                     /locus_tag="JNZ27_000303"
     CDS             49994..50560
                     /gene="kdsC"
                     /locus_tag="JNZ27_000303"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY8662779.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPIMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50557..51132
                     /gene="lptC"
                     /locus_tag="JNZ27_000304"
     CDS             50557..51132
                     /gene="lptC"
                     /locus_tag="JNZ27_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY8662780.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51101..51658
                     /gene="lptA"
                     /locus_tag="JNZ27_000305"
     CDS             51101..51658
                     /gene="lptA"
                     /locus_tag="JNZ27_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY8662781.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVIGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51665..52390
                     /gene="lptB"
                     /locus_tag="JNZ27_000306"
     CDS             51665..52390
                     /gene="lptB"
                     /locus_tag="JNZ27_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY8662782.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52438..53871
                     /gene="rpoN"
                     /locus_tag="JNZ27_000307"
     CDS             52438..53871
                     /gene="rpoN"
                     /locus_tag="JNZ27_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY8662783.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53894..54181
                     /gene="hpf"
                     /locus_tag="JNZ27_000308"
     CDS             53894..54181
                     /gene="hpf"
                     /locus_tag="JNZ27_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY8662784.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54299..54790
                     /gene="ptsN"
                     /locus_tag="JNZ27_000309"
     CDS             54299..54790
                     /gene="ptsN"
                     /locus_tag="JNZ27_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY8662785.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPISFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54836..55690
                     /gene="rapZ"
                     /locus_tag="JNZ27_000310"
     CDS             54836..55690
                     /gene="rapZ"
                     /locus_tag="JNZ27_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY8662786.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55687..55959
                     /gene="npr"
                     /locus_tag="JNZ27_000311"
     CDS             55687..55959
                     /gene="npr"
                     /locus_tag="JNZ27_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY8662787.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56172..56804
                     /gene="yrbL"
                     /locus_tag="JNZ27_000312"
     CDS             56172..56804
                     /gene="yrbL"
                     /locus_tag="JNZ27_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY8662788.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="JNZ27_000313"
     CDS             complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="JNZ27_000313"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY8662789.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="JNZ27_000314"
     CDS             complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="JNZ27_000314"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY8662790.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="JNZ27_000315"
     CDS             complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="JNZ27_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY8662791.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60841..61770)
                     /locus_tag="JNZ27_000316"
     CDS             complement(60841..61770)
                     /locus_tag="JNZ27_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY8662792.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62445..66905
                     /gene="gltB"
                     /locus_tag="JNZ27_000317"
     CDS             62445..66905
                     /gene="gltB"
                     /locus_tag="JNZ27_000317"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY8662793.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAAHRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66918..68336
                     /locus_tag="JNZ27_000318"
     CDS             66918..68336
                     /locus_tag="JNZ27_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8662794.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68520..68999
                     /locus_tag="JNZ27_000319"
     CDS             68520..68999
                     /locus_tag="JNZ27_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY8662795.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSAELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16847 a        18751 c        17154 g        16254 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgccagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccaaccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ctacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaaccgggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgg acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aataatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac ccggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ttgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    28261 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    28321 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    28381 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    28441 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    28501 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    28561 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    28621 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    28681 atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    28741 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtatagata
    28801 gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    28861 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    28921 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    28981 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac aacttcatcc
    29041 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    29101 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    29161 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    29221 agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca
    29281 ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt tgttgaatac
    29341 tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt
    29401 atatcagaca tgtttttcaa ctctgaacca tatcagaccg aaacactgtt agttttttct
    29461 ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
    29521 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    29581 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    29641 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca
    29701 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat cagaccaaga
    29761 ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    29821 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    29881 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    29941 cagctggctg agtttgttcg gttttcgccg gtgcactcag attttcccat tcgctacctc
    30001 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    30061 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    30121 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    30181 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    30241 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    30301 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    30361 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    30421 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    30481 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    30541 cacgcgaatt aacggttcag tgccggattt acgcagcaac acgcgtccac gattgcccag
    30601 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    30661 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    30721 gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    30781 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
    30841 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    30901 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    30961 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    31021 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    31081 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    31141 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    31201 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    31261 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    31321 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
    31381 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    31441 cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    31501 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    31561 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    31621 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    31681 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    31741 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    31801 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    31861 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    31921 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    31981 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    32041 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    32101 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    32161 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    32221 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    32281 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    32341 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    32401 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    32461 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    32521 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    32581 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    32641 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    32701 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    32761 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    32821 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    32881 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    32941 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    33001 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    33061 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    33121 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    33181 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    33241 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    33301 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    33361 aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    33421 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    33481 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    33541 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    33601 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    33661 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    33721 ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    33781 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    33841 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    33901 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    33961 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    34021 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    34081 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    34141 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    34201 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    34261 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    34321 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    34381 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    34441 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    34501 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    34561 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    34621 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    34681 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    34741 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    34801 ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    34861 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    34921 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    34981 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    35041 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    35101 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    35161 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    35221 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    35281 aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa catatttatc
    35341 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    35401 actttcccat ggggagaact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    35461 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    35521 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    35581 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    35641 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaacaaaaa
    35701 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    35761 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    35821 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    35881 aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc tttctttaca
    35941 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    36001 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    36061 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    36121 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    36181 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgtttg
    36241 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    36301 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    36361 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    36421 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    36481 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    36541 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatggt
    36601 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    36661 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    36721 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    36781 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg tcggcccccg
    36841 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
    36901 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    36961 aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    37021 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    37081 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    37141 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct gccgccatag
    37201 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt gatatggctt
    37261 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    37321 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaggcgac ctgaaccgct
    37381 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttggcgtgc
    37441 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
    37501 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    37561 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    37621 tgatcgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    37681 ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    37741 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    37801 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    37861 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    37921 gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    37981 gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt tctggctatg
    38041 cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    38101 tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca tggccggggt
    38161 ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac gccttatccg
    38221 gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg ataagtgtag
    38281 cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg aactcaacgc
    38341 cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct tcagcaatct
    38401 cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg
    38461 cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat
    38521 ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcttcc cagcccagcg
    38581 cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt
    38641 tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctcgctga
    38701 taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt
    38761 gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt
    38821 ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca
    38881 ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc actttcggtt
    38941 tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac
    39001 cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt
    39061 tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg cccagaccgt
    39121 gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca cccatggttt
    39181 caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc acgtctttac
    39241 cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca cggaaagatt
    39301 tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac
    39361 caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga
    39421 agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa
    39481 acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat
    39541 gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca
    39601 cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcgggcc
    39661 aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg
    39721 cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca tatcctacca
    39781 aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca
    39841 ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt aaaaacagga
    39901 tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa
    39961 ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata
    40021 tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc aatgccccta
    40081 caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa
    40141 ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc
    40201 tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa atcaaccagc
    40261 gtggcttacg aaacacgcgt aatggcggca acctcttctt caccgcaaga atggcaccaa
    40321 ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca
    40381 gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta agcgccaaaa
    40441 gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tttgcggatt
    40501 aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg
    40561 cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt
    40621 tacggttttt cgggccttta acttcgaatt tcactttacc gtctgcttta gcaaacagag
    40681 tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa
    40741 cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc aggcgtttag
    40801 cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc
    40861 tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg
    40921 gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc
    40981 acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat
    41041 tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt
    41101 tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc
    41161 accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat
    41221 tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt
    41281 gacaagtgct acagtcaata cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc
    41341 tgtgccattt tttcagtaca attaccctat attcctaacc ataaacccta agttgccttt
    41401 gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc
    41461 aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat
    41521 tccgacgtcc aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt
    41581 attcgtccga tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat
    41641 gtcaccattg ctgccctgat cgagtttatc cacacggcga ctctgctaca cgacgacgtt
    41701 gtggatgaat cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc
    41761 gccagcgtgc tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc
    41821 ggttcactca aagtgctgga agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa
    41881 gttctgcaac tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt
    41941 atctatagca aaaccgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct
    42001 ggctgtacgc cggaggagga gaaaggcctg caggattatg ggcgctatct cggcactgct
    42061 ttccagttga tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac
    42121 gtcggcgacg atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat
    42181 ggcacaccag aacaggcaca gatgatccgt accgccatcg aacaaggtaa cggtcgccat
    42241 cttctggaac cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag
    42301 cgcgccgagg aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacacccct
    42361 tggcgagaag cactcatcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct
    42421 cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat tccataaaaa cactaattca
    42481 gcgctataac acctgaaaag aagggcgatg aatctttata tattctgaat attcacactc
    42541 tttacaggaa ctttttagcg caataggcca tcaggagtat agtgatgccc gacagaagaa
    42601 gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg
    42661 actggcatcc cgctgacatc attgcaggtt tgcgcaagaa aggaacgtca atggcggcgg
    42721 aatctcgcag aaatggtttg agttcctcaa cgctggcaaa cgcattatcg cgcccatggc
    42781 cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat
    42841 cacgctacca tgatccgcgg acccatgagt ttatcgacag aacgcagttg atgcgtagct
    42901 acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct
    42961 cagacgatta ctcgcctttc acacgctcaa tatttgcacc taaagcgcgc agtttgtctt
    43021 caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg
    43081 caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg
    43141 caccagaaag tttttcaaca ccatgacaaa taacggtatt gctttcgatt tcggcgtgcg
    43201 cgcccatacg gctcagctct ggcacatgca taaagcggtt ttcaaagacc gtttcggtga
    43261 tgaacccggt cccttcagcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg
    43321 ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat
    43381 ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc agtttcgcca
    43441 gcacggcgtc cagagtatct ggctgcgcgt tacggcagat aattttgcca cgagagatcg
    43501 ccgccgccac caggaaagta ccggtttcga tacgatccgg cagcacgcga tagacaccgc
    43561 cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg
    43621 cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt
    43681 caataatcgt ggtgccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa
    43741 cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa
    43801 cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat
    43861 caaccggacg agcaccgatc gtacaaccgc caggtagtga aacttgcccc tgaccaaagc
    43921 gcgctaccag cggccccagc gcccagatag aagcacgcat ggttttaacc agatcgtaag
    43981 gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt
    44041 tcgcacccag ctggcttagc agtttcattg atgtatcgac gtctttcagt ttcgggacgt
    44101 tctggatctc taccggttct tccgccagta gtgcggcaaa aagaataggc agagcagcat
    44161 ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt
    44221 tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag
    44281 ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg
    44341 acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct
    44401 gttttttaac ccgactcata ccgtcaaaca actcacccac ggcaataacc tgaaagtgac
    44461 tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa
    44521 tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga
    44581 gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga
    44641 cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac
    44701 gaggcagaac atcagcaggc aaattataca atttcgcgag ggtatacact ttgtcgttta
    44761 ccccctgaag cgtcacgttg ttgccctgct ttttcgccag atcgataaga tggagcagca
    44821 gtgccagtcc ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaatcccct
    44881 tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac gtcctgatcc agctctccgg
    44941 ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc
    45001 ttccagagtg attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc
    45061 tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc
    45121 agcaatcatg tcgtaagctt gccagtttcc cgtctgggag tttttacgcc actggaagtc
    45181 cagacgcacc ggcggacggc cattcgggtc aataatggta acgcgaatag gcacaatggt
    45241 tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat acatcgccag
    45301 cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg cttcacgttg
    45361 agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg
    45421 tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat
    45481 ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt
    45541 ataaggattg gtctggtctg ccgcggttgc cgcactcaga ggtgcaatca ccagcaaagc
    45601 gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac
    45661 aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt
    45721 gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga
    45781 cttggtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc
    45841 aaaaccgacg tttaatgcca gatattgttc tcccagcagg ccggaagtac gaatgctcag
    45901 cgaactggta tcaggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgcggcag
    45961 ataggttttc gggtccagcg taatatccgc cacccggccc acaacaacac caccaatgct
    46021 gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt
    46081 cgattcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gcgccgccag
    46141 cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgaactc
    46201 aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt
    46261 ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc
    46321 cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt
    46381 tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat
    46441 caatgccttt ccaactgacg ccgaccagcg atccgcccca gatccccacg gcgacgaaga
    46501 taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac
    46561 gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc
    46621 cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa caacagcgcg gcaaccaccg
    46681 gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcgctat
    46741 aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa
    46801 ctacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc agcagcggcg
    46861 catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc
    46921 cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca
    46981 tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac
    47041 ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgtc
    47101 tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga
    47161 tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc
    47221 gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca
    47281 acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg cgccagcgct
    47341 gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc tccacgcagc
    47401 cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg aagttgggta
    47461 tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg
    47521 gactgaaata acatgctcat ccgtttacgc actgtataca ggcgcgaacg agacatcgcc
    47581 ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc
    47641 agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct
    47701 cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca
    47761 tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt
    47821 gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc
    47881 cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta
    47941 agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg
    48001 acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag
    48061 tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct
    48121 gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct
    48181 tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat
    48241 tatcaatata ttgctgatcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc
    48301 cgatgttctg cgccgtgaat tacccttaat gttgttggtc agcgtggtgg ccggttccgt
    48361 actctatgac ggacaactta gtcgaagcga tggtatcttt ctcctctttc tggctgtgct
    48421 atggctgctg ttcattgtta aacttgcacg tcaggggact gacagcctga ccagagagca
    48481 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    48541 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    48601 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagtct
    48661 gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    48721 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    48781 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    48841 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    48901 ggtattatta actggcggat ttatcatatg gctggcgatg ttgtactggt tatcgccaat
    48961 actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact
    49021 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    49081 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    49141 ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg
    49201 ccagcaccgg tacaccttca tttttcgtcc atcctagtga agccgcgcat ggtgatttag
    49261 gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa
    49321 tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    49381 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    49441 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    49501 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    49561 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    49621 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    49681 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    49741 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    49801 gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctgaccgttg
    49861 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    49921 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    49981 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    50041 cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt
    50101 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    50161 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    50221 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    50281 tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg ctggaaaaat tggcgattgc
    50341 cccggaaaat gtggcttatg ttggcgatga tctcatcgac tgcccgataa tggaaaaagt
    50401 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    50461 gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc
    50521 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    50581 atcattgtgc tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac
    50641 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    50701 acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa
    50761 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    50821 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    50881 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cagactctca acttcgcaga
    50941 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    51001 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    51061 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    51121 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    51181 cgtaatcgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    51241 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    51301 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    51361 cggatacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    51421 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    51481 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    51541 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc
    51601 gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    51661 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    51721 agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg
    51781 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    51841 catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg
    51901 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    51961 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    52021 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    52081 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    52141 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgata ttaaacgcat
    52201 cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    52261 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    52321 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    52381 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    52441 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag
    52501 gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg
    52561 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    52621 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    52681 ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc
    52741 ggtgactaca ttgacgacga gctgccggtc taccagggcg aaacgacgca gaccttgcag
    52801 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    52861 acctctatcg tcgatgccgt tgatgaaacc ggttatctga ctgtcccgct ggaagatatt
    52921 ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg
    52981 atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc
    53041 caactctccc aattcgataa gaccacgccg tggctggaag aggccagatt gatcattagc
    53101 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    53161 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    53221 cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    53281 aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    53341 cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt tatccgcagc
    53401 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    53461 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat
    53521 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    53581 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    53641 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    53701 gcactggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    53761 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    53821 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga
    53881 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    53941 gcgcgaattt gttacagcta aatttgccaa acttgagcaa tattttgacc gaatcaacca
    54001 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    54061 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    54121 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    54181 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    54241 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    54301 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    54361 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    54421 acaacttagc ctaccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    54481 cagtaccggt atcggcaatg gtattgccat tccgcatggc aaacttgaag aagatactct
    54541 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata tctttcgatg ccatcgacaa
    54601 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    54661 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    54721 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    54781 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    54841 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    54901 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    54961 aactctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    55021 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    55081 actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    55141 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgattt
    55201 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    55261 cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgaccat
    55321 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    55381 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    55441 taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    55501 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    55561 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    55621 agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    55681 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    55741 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    55801 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    55861 aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc
    55921 gttatcgccc tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca
    55981 actcccttca agctcccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac
    56041 gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa accttaagag
    56101 gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    56161 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    56221 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    56281 atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt ggtcgccggt
    56341 taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat tgcggaaccg
    56401 gttatgtcta cgatgtgatt gccgattttg acggcaagcc ttccattacg ctgaccgaat
    56461 ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta ctgaaacagc
    56521 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    56581 tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa
    56641 gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag caagagcggt
    56701 tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    56761 ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt
    56821 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctac
    56881 gcacgtagcc gtcaggcgtg gagactttga aacgaagtgg attaggtaat acagctgcca
    56941 gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    57001 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    57061 tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc agcccttttc
    57121 gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga gaaatcgttg
    57181 aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc tcaatggaag
    57241 cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc accgccagtc
    57301 ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg taacgaaaat
    57361 tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag ggaacaggtg
    57421 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    57481 aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    57541 cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    57601 catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    57661 acacggcaca tgctccgcgc ccatctcttc cagtacttct gcggtatcga tatcagtacc
    57721 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    57781 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    57841 aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg cgccaacccc
    57901 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    57961 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc
    58021 agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    58081 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    58141 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt
    58201 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    58261 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt
    58321 gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca
    58381 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    58441 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    58501 tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    58561 gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    58621 tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc atcttctcaa
    58681 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    58741 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    58801 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga accgaaagcg
    58861 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    58921 tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac
    58981 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    59041 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    59101 ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca
    59161 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    59221 ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa
    59281 ccgtaatatc gccgcccata tttttcgcca gacgatgaga aacggccaga ccaataccgg
    59341 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt
    59401 tatccagctc atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat
    59461 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    59521 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    59581 gtaatggcag cgtaggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    59641 agagattttc cagatcggca aggaagctgg tgaaatcaac cggctgatta tcaagctgga
    59701 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    59761 cggccgaaac atggatagtt ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    59821 gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg tgactgatgg
    59881 tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac
    59941 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    60001 acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt
    60061 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg acttttgcgg
    60121 cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac cagttgtttt tcgctttttc
    60181 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    60241 aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct
    60301 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    60361 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    60421 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    60481 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    60541 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    60601 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    60661 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    60721 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    60781 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    60841 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    60901 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gcggagcaag
    60961 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    61021 gcgaatcatt tctccggcag tgagcgtata atcctccagt tcaataccgt tcaaacgccc
    61081 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    61141 gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    61201 gccaggcagg ccgacaatta agtgggaaca caccttcagc ccgcgctgac gtgccagctg
    61261 ggttgtacgc tgataacagg caaaatcatg gccgcggttg atgcgatgca gtgttttgtc
    61321 gtgggcggtt tgtagcccca gctccagcca cacttcgtaa ccctggtcct tatattcgca
    61381 aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac acaaaccgac
    61441 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    61501 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    61561 ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg caaacgaggc
    61621 aacattacag aatgtgcagc caccacgccc gatggtaccg tcacggttag ggcagctaaa
    61681 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    61741 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    61801 caaagcctgc catttttagc tcaattcggc gatgacctag atcaatcgtc ccggcctgct
    61861 tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    61921 ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca aaaacagtag
    61981 catgaaacgt cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg
    62041 caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct ctaacgactc
    62101 tccttttagc cttaaagata aaatctattt taatttcagc catttaataa agaattttgc
    62161 gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata
    62221 agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta
    62281 attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc
    62341 gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg
    62401 gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat
    62461 cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc
    62521 acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc
    62581 tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt
    62641 ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgct gtcgggatgc
    62701 tcttcctgaa taaagatcct gaactcgccg ctgccgcaca ccgcatcgtt gaagaagaac
    62761 tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc
    62821 tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg
    62881 caggctggcg tccacgcgat atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa
    62941 agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct
    63001 ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt gcggacctgc
    63061 gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct
    63121 ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac accatcaccg
    63181 gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg atccctgacc
    63241 tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga ctccagttcc atggataaca
    63301 tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgc ctgttagtgc
    63361 cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta
    63421 actccatgca tatggagccg tgggatggtc cggcgggcat cgtgatgtcc gatggtcgct
    63481 tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc attaccaaag
    63541 acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg
    63601 tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat cgacactcgc agtgggcgta
    63661 ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga
    63721 tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta
    63781 gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg
    63841 aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga
    63901 tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact
    63961 tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg
    64021 ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc
    64081 aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc aaacagctca
    64141 cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacta
    64201 aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa agccgaaaaa atggtacgta
    64261 gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc taaagatcgc ctgccggttc
    64321 cggctccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg
    64381 atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc
    64441 tgggtttcgg cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg
    64501 tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca
    64561 tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc
    64621 gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc ctgtgcttcc
    64681 agggcgcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag gatctgctga
    64741 atctgtcgaa acgtgcctgg ctggcgcgca agccgattag ccagggcggc ctgctgaaat
    64801 acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg
    64861 cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt aatgagcgtc
    64921 cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg
    64981 ctgatgttga accggcaagc gaactgttta aacgctttga taccgccgcg atgtctatcg
    65041 gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaatagc atcggcggta
    65101 actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc
    65161 gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg
    65221 acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg
    65281 gcgacaaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga gtgacgctga
    65341 tctccccgcc gccgcaccac gatatctact ctatcgaaga cttagcgcag cttattttcg
    65401 acctcaagca ggttaacccg aaagcgatga tctccgttaa gctggtgtcc gaaccaggcg
    65461 ttggcaccat cgcgactggc gtggcgaaag cttatgcgga cttgatcacc atcgcgggct
    65521 atgacggcgg caccggcgca agcccgctgt cgtcagtgaa atatgcaggc tgcccgtggg
    65581 aactcggtct ggtggaaacc caacaggcgc tggttgccaa cggcttgcgt cataaaattc
    65641 gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat catcaaagcg gcgattctcg
    65701 gcgcagaaag cttcggcttc ggcactggcc caatggtggc gctcggctgt aaatatctgc
    65761 gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta
    65821 agaatcacta tcacggcctg ccgttcaagg tgactaatta ctttgagttt atcgcccgtg
    65881 aaacccgcga gctgatggca cagctgggag taacgcgtct ggtggatctg attggtcgca
    65941 ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga
    66001 agctgctgga gactgccgag ccacatgcag gtaagtcact ctactgcact gaaaacaacc
    66061 cgccgtttga taacggccta ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg
    66121 atgaacgcca gagcaaaacc ttctggttcg atatccgcaa caccgaccgt tctgtcggcg
    66181 cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccta
    66241 tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg
    66301 tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg gcgggcggct
    66361 taatcgccat tcatcctccg gttggttccg ccttccgcag ccatgaagca agcattatcg
    66421 gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg
    66481 aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg
    66541 gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc gttaacttcg
    66601 gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac
    66661 gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga tctggcgatc catgaagagc
    66721 atctgcgcgg tcttatcacc gagcatgttc agcataccgg ttctcagcgc ggtgaagaga
    66781 ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct ggttaaaccg aagtccagtg
    66841 atgtaaaagc actgctgggt caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc
    66901 agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg
    66961 atccgccaaa gaaaccgctg aagatccgta aaattgagtt tgttgaaatt tacgagccgt
    67021 tttccgaagg ccaggccaaa gcgcaggctg accgctgtct gtcgtgcggc aacccatact
    67081 gcgagtggaa atgcccggta cacaactaca tcccaaactg gctgaagctc gccaacgagg
    67141 ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa cacactgccg gaagtatgcg
    67201 gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagttcg
    67261 gtgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc gaaatgggct
    67321 ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt atcggtgcag
    67381 gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct
    67441 tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg
    67501 aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac
    67561 tcaataccga agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg
    67621 tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg
    67681 atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg
    67741 gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggta gtacttggcg
    67801 gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta
    67861 cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg
    67921 cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt gaagtgaacg
    67981 gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca
    68041 aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg gcagatgcgg
    68101 tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa cacagcgtcg
    68161 agctggattc acaaggccgc atcatcgctc cggaaggcaa cgacaacgct ttccagacca
    68221 gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtcgttaccg
    68281 ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa ctggctggaa gtttaagctc
    68341 tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt ctttctggaa
    68401 atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt gttaccggta
    68461 gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag ggacagataa
    68521 tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac ggcattattc
    68581 atctggtcga tagcgcccga acggaaacgg tacgtagcgt taacgcgctg atgaccgcga
    68641 cgtactggga aattggccga cggattgtcg aatttgaaca aggtggtgag gccagagcag
    68701 cttatggtgc acagttgatt gatcggctat cgaaggattt aagtcgacgg tataaacgtg
    68761 gggtctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg
    68821 aaattcgcca gacagtgtct gccgaattaa caccattggg aattccgcag acaccatctg
    68881 cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga ggctgtttga
    68941 tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca tgcaaataaa
    69001 cgaagg
//
LOCUS       DADMFL010000005        68078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.005, whole genome shotgun sequence.
ACCESSION   DADMFL010000005 DADMFL010000000
VERSION     DADMFL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.005"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..247
                     /locus_tag="JNZ27_000320"
     CDS             <1..247
                     /locus_tag="JNZ27_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662796.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNZ27_000321"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNZ27_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY8662797.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNZ27_000322"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNZ27_000322"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8662798.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JNZ27_000323"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JNZ27_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY8662799.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTDGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JNZ27_000324"
     CDS             complement(3258..3542)
                     /locus_tag="JNZ27_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662800.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNZ27_000325"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNZ27_000325"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY8662801.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JNZ27_000326"
     CDS             complement(4443..5651)
                     /locus_tag="JNZ27_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8662802.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /locus_tag="JNZ27_000327"
     CDS             5735..6271
                     /locus_tag="JNZ27_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8662803.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6455..8131)
                     /locus_tag="JNZ27_000328"
     CDS             complement(6455..8131)
                     /locus_tag="JNZ27_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY8662804.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQISAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWP"
     gene            8401..8778
                     /locus_tag="JNZ27_000329"
     CDS             8401..8778
                     /locus_tag="JNZ27_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662805.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNZ27_000330"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNZ27_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY8662806.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JNZ27_000331"
     CDS             9225..9512
                     /locus_tag="JNZ27_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY8662807.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JNZ27_000332"
                     /pseudo
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JNZ27_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JNZ27_000333"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JNZ27_000333"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY8662808.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JNZ27_000334"
     CDS             11269..11745
                     /locus_tag="JNZ27_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY8662809.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="JNZ27_000335"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="JNZ27_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY8662810.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="JNZ27_000336"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="JNZ27_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY8662811.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="JNZ27_000337"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="JNZ27_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY8662812.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="JNZ27_000338"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="JNZ27_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY8662813.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="JNZ27_000339"
     CDS             16302..16790
                     /locus_tag="JNZ27_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY8662814.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="JNZ27_000340"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="JNZ27_000340"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY8662815.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNZ27_000341"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNZ27_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8662816.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNZ27_000342"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNZ27_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY8662817.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNZ27_000343"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNZ27_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY8662818.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNZ27_000344"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNZ27_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8662819.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNZ27_000345"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNZ27_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY8662820.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="JNZ27_000346"
     CDS             complement(22242..22757)
                     /locus_tag="JNZ27_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8662821.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLNDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="JNZ27_000347"
     CDS             23406..25175
                     /locus_tag="JNZ27_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8662822.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRDVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYSISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="JNZ27_000348"
     CDS             25386..25709
                     /locus_tag="JNZ27_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY8662823.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /locus_tag="JNZ27_000349"
     CDS             25706..26536
                     /locus_tag="JNZ27_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY8662824.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="JNZ27_000350"
     CDS             complement(26533..27546)
                     /locus_tag="JNZ27_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8662825.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="JNZ27_000351"
     CDS             complement(27645..29075)
                     /locus_tag="JNZ27_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8662826.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JNZ27_000352"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JNZ27_000352"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY8662827.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JNZ27_000353"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JNZ27_000353"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY8662828.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGCFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JNZ27_000354"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JNZ27_000354"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY8662829.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JNZ27_000355"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JNZ27_000355"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY8662830.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JNZ27_000356"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JNZ27_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY8662831.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JNZ27_000357"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JNZ27_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY8662832.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="JNZ27_000358"
     CDS             37227..38885
                     /locus_tag="JNZ27_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY8662833.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="JNZ27_000359"
     CDS             complement(38882..39838)
                     /locus_tag="JNZ27_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY8662834.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="JNZ27_000360"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="JNZ27_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY8662835.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="JNZ27_000361"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="JNZ27_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY8662836.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="JNZ27_000362"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="JNZ27_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY8662837.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="JNZ27_000363"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="JNZ27_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY8662838.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="JNZ27_000364"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="JNZ27_000364"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY8662839.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46637..46724)
                     /locus_tag="JNZ27_000365"
     tRNA            complement(46637..46724)
                     /locus_tag="JNZ27_000365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46688..46690),aa:Ser,seq:gga)
     gene            complement(46978..47196)
                     /gene="infA"
                     /locus_tag="JNZ27_000366"
     CDS             complement(46978..47196)
                     /gene="infA"
                     /locus_tag="JNZ27_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY8662840.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47481..48185)
                     /gene="aat"
                     /locus_tag="JNZ27_000367"
     CDS             complement(47481..48185)
                     /gene="aat"
                     /locus_tag="JNZ27_000367"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY8662841.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48227..49948)
                     /gene="cydC"
                     /locus_tag="JNZ27_000368"
     CDS             complement(48227..49948)
                     /gene="cydC"
                     /locus_tag="JNZ27_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY8662842.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(49949..51715)
                     /gene="cydD"
                     /locus_tag="JNZ27_000369"
     CDS             complement(49949..51715)
                     /gene="cydD"
                     /locus_tag="JNZ27_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY8662843.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51838..52803)
                     /gene="trxB"
                     /locus_tag="JNZ27_000370"
     CDS             complement(51838..52803)
                     /gene="trxB"
                     /locus_tag="JNZ27_000370"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY8662844.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53348..53842
                     /gene="lrp"
                     /locus_tag="JNZ27_000371"
     CDS             53348..53842
                     /gene="lrp"
                     /locus_tag="JNZ27_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY8662845.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            53977..58044
                     /gene="ftsK"
                     /locus_tag="JNZ27_000372"
     CDS             53977..58044
                     /gene="ftsK"
                     /locus_tag="JNZ27_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY8662846.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQLVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58199..58810
                     /gene="lolA"
                     /locus_tag="JNZ27_000373"
     CDS             58199..58810
                     /gene="lolA"
                     /locus_tag="JNZ27_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY8662847.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58821..60164
                     /locus_tag="JNZ27_000374"
     CDS             58821..60164
                     /locus_tag="JNZ27_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY8662848.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKTGEKLAWLAEQDQNSPIKRYR"
     gene            60255..61547
                     /gene="serS"
                     /locus_tag="JNZ27_000375"
     CDS             60255..61547
                     /gene="serS"
                     /locus_tag="JNZ27_000375"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY8662849.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61787..64231
                     /gene="dmsA"
                     /locus_tag="JNZ27_000376"
                     /pseudo
     CDS             61787..64231
                     /gene="dmsA"
                     /locus_tag="JNZ27_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64242..64859
                     /gene="dmsB"
                     /locus_tag="JNZ27_000377"
     CDS             64242..64859
                     /gene="dmsB"
                     /locus_tag="JNZ27_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8662850.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64861..65724
                     /locus_tag="JNZ27_000378"
     CDS             64861..65724
                     /locus_tag="JNZ27_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY8662851.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65760..66386)
                     /locus_tag="JNZ27_000379"
     CDS             complement(65760..66386)
                     /locus_tag="JNZ27_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8662852.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66701..67849
                     /locus_tag="JNZ27_000380"
     CDS             66701..67849
                     /locus_tag="JNZ27_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8662853.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16913 a        16826 c        17829 g        16510 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgacgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcatgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctga
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctgtttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa gcgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggataccta gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcatc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcac gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcattcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgtg
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata ttctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttatcaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaaca
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg caaaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttattaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46561 accatgttca ctgccgtaca gacagataaa atgagaaaaa aaagctcgca ctttcgtacg
    46621 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46681 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46741 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46801 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46861 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46921 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    46981 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47041 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47101 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47161 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47221 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47281 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47341 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47401 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47461 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47521 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47581 atttcgcagg caccaagcga tgcagtgtga tcgttaagga cctggcagtc gataagctta
    47641 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    47701 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47761 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47821 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47881 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    47941 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48001 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48061 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48121 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48181 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48241 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48301 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48361 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48421 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48481 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48541 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48601 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48661 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48721 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48781 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48841 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48901 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    48961 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49021 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49081 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49141 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49201 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49261 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49321 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49381 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49441 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49501 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49561 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49621 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49681 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49741 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49801 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49861 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49921 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    49981 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50041 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50101 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50161 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50221 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50281 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50341 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50401 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50461 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50521 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50581 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50641 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50701 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50761 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50821 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50881 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    50941 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51001 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51061 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51121 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51181 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51241 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51301 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51361 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51421 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51481 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51541 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51601 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51661 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51721 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51781 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51841 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51901 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    51961 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52021 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52081 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52141 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52201 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52261 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52321 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52381 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52441 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52501 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52561 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52621 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52681 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52741 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52801 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52861 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52921 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    52981 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    53041 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53101 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53161 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53221 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53281 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53341 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53401 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53461 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53521 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53581 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53641 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53701 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53761 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53821 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53881 tcctgttaat tcatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    53941 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54001 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54061 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54121 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54181 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54241 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat
    54301 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54361 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54421 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54481 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54541 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54601 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54661 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54721 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54781 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac
    54841 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54901 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    54961 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55021 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55081 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55141 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55201 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55261 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55321 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55381 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55441 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55501 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55561 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55621 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55681 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55741 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55801 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55861 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55921 aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    55981 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56041 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56101 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56161 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56221 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56281 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56341 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56401 caaccggttg cgccacagca gcaatatcag cagccgcaac aactagttgc gccgcagccg
    56461 cagtatcagc agccacaaca gccagttgcg ccacagccgc aggataccct gcttcatccg
    56521 ctgttgatgc gtaatggcga cagccgtccg ttgcacaaac cgacgacgcc gctgccttct
    56581 ctggatttgc tgacaccgcc gccgagcgaa gtggagccgg tagatacctt tgcgcttgaa
    56641 caaatggcgc gtctggtgga agcgcgtctg gctgatttcc gtattaaagc cgatgtcgtt
    56701 aattactctc cggggccagt tatcactcgc tttgaactta acctggctcc tggcgtaaaa
    56761 gcggcgcgca tttctaactt gtcacgggac cttgcccgtt cactttcgac ggtggcggtg
    56821 cgtgtcgttg aagttattcc tggcaaaccc tatgtaggtc tggagttacc aaataaaaaa
    56881 cgacaaaccg tttatctgcg cgaagttttg gataacgcca aattccgcga taatccgtcg
    56941 ccattaaccg tggtgctggg taaagatatc gccggtgagc cggtggttgc cgatctggcg
    57001 aaaatgccgc acttgttggt tgcggggact accggttccg gtaaatctgt cggtgtgaac
    57061 gcgatgatcc tgagcatgct ttataaagca cagccagaag atgtgcgttt catcatgatc
    57121 gacccgaaaa tgctggagct ttcggtttat gaaggcattc cgcacctgtt aacggaagtc
    57181 gttactgata tgaaagatgc cgccaacgcg ctgcgctggt gtgttaacga gatggagcgt
    57241 cggtataaac tgatgtctgc gctgggtgtg cgtaatctgg cgggttataa cgaaaaaatt
    57301 gctgaagccg atcgcatgat gcgtccgatt ccagacccgt actggaagcc gggtgacagt
    57361 atggatgccc agcatccggt gctgaaaaaa gaaccataca ttgtcgtgtt ggttgacgaa
    57421 tttgccgacc tgatgatgac ggtaggtaaa aaagtggaag agctgatagc acgtctggcg
    57481 caaaaagccc gtgccgcggg tatccacctc gtactggcaa ctcagcgtcc atcggttgat
    57541 gttattactg gtctgattaa agcgaatatt ccgacccgta tcgcctttac cgtatccagt
    57601 aagattgact cacgtaccat tcttgatcag gctggcgcgg aatcactgct gggtatgggg
    57661 gatatgctct actctgggcc gaactccacg ttgccggtac gtgtccatgg tgcttttgtt
    57721 cgcgatcagg aagttcatgc cgtggtgcag gactggaaag cgcgtggtcg cccacagtat
    57781 gttgatggca tcacctccga cagcgaaagc gaaggtggtg cgggtggttt cgatggcgct
    57841 gaagaactgg atccgttgtt cgatcaggcg gtgcagtttg tcactgaaaa acgcaaagcg
    57901 tcaatttctg gcgtacagcg tcagttccgc attggttata accgtgcagc gcgtattatc
    57961 gaacagatgg aagcgcaggg gattgtcagc gaacaggggc acaacggtaa tcgtgaagtg
    58021 ctggccccac cgccgtttga ctaattaatg cattgccgga taaggcgcgg tagcgtcgca
    58081 tccggcactc tatcaactga aaattcagta ttttcttctt tcctcaagct gattattagc
    58141 ctggaataga gagtagaggg aactcccgat cgggagtgac gtaatttgag gaataatgat
    58201 gaaaaaaatt gctatcacct gtgcattact ctcaagctta gtagcaagca gcgtttgggc
    58261 tgatgccgca agcgatctga aaagccgcct ggataaagtc agcagcttcc acgccagctt
    58321 cacacaaaaa gtgactgacg gtagcggcgc ggcggtgcag gaaggtcagg gcgatctgtg
    58381 ggtgaaacgt ccaaacttat tcaactggca tatgacacaa cctgatgaaa gcattctggt
    58441 ttctgacggt aaaacactgt ggttctataa cccgttcgtt gagcaagcta cggcaacctg
    58501 gctgaaagat gccaccggta atacgccgtt tatgctgatt gcccgcaacc agtccagcga
    58561 ctggcagcag tacaatatca aacagaatgg cgatgacttt gtcctgacgc cgaaagccag
    58621 caatggcaat ctgaagcagt tcaccattaa cgtgggacgt gatggcacaa tccatcagtt
    58681 tagcgcggtg gagcaggacg atcagcgcag cagttatcag ctgaaatccc agcaaaatgg
    58741 ggctgtggat gcagcgaaat ttaccttcac cccgccgcaa ggcgtcacgg tagatgatca
    58801 acgtaagtag aggcacctga gtgagcaatc tgtcgctcga tttttcggat aatacttttc
    58861 aacctctggc cgcgcgtatg cggccagaaa atttagcaca gtatatcggt cagcaacatt
    58921 tgctggctgc ggggaagccg ttgccgcgcg ctatcgaagc cgggcatttg cattctatga
    58981 tcctctgggg gccaccgggt accggcaaaa caaccctcgc tgaagtgatt gcccgctatg
    59041 cgaacgctga tgtggaacgt atttctgccg tcacctctgg cgtgaaagag attcgcgagg
    59101 cgatcgagcg cgcccggcaa aaccgcaatg caggtcgccg cactattctt tttgttgacg
    59161 aagttcaccg tttcaacaaa agccagcagg atgcatttct gccacatatt gaagacggca
    59221 ccatcacttt tattggcgca accactgaaa acccgtcgtt tgagcttaat tcggcactgc
    59281 tttcccgtgc ccgtgtttat ctgttgaaat ccctgagtac agaggatatt gagcaagtac
    59341 taactcaggc gatggaagac aaaacccgtg gctatggtgg tcaggatatt gttctgccag
    59401 atgatacacg acgtgccatt gcagaactgg tgaatggcga cgcgcgccgg gcgttaaata
    59461 cgctggaaat gatggcggat atggccgaag tctatgatag cggtaagcgg gtcctgaagc
    59521 ctgaattact gaccgaaatc gccggtgaac gtagcgcccg ctttgataac aaaggcgatc
    59581 gcttttacga tctaatttcc gcactgcata agtcggtacg tggtagcgca cccgatgcgg
    59641 cgctgtactg gtatgcgcga attattaccg ctggtggcga tccgttatat gtcgcgcgtc
    59701 gctgtctggc gattgcgtct gaagacgtcg gtaatgccga tccacgggcg atgcaggtgg
    59761 caattgcggc ctgggattgc tttactcgcg ttggcccggc ggaaggtgaa cgcgccattg
    59821 ctcaggcgat tgtttacctg gcctgcgcgc caaaaagcaa cgctgtctac actgcgttta
    59881 aagccgcgct ggccgatgct cgcgaacgcc cggattatga cgtgccggtt catttgcgta
    59941 atgcgccgac gaaattaatg aaggaaatgg gctacgggca ggaatatcgt tacgctcatg
    60001 atgaagcaaa cgcttatgct gccggtgagg tttacttccc gccggaaata gcacaaacac
    60061 gctattattt cccgacaaac aggggccttg aaggcaagac tggcgaaaag ctcgcctggc
    60121 tggctgaaca ggatcaaaat agccccataa aacgctaccg ttaatgttat cgttgcggta
    60181 atgttgttac tgtatccctg tggtcgcagg ctgtggccac atctcccatt taattcgata
    60241 agcacaggat aagcatgctc gatcccaatc tgctgcgtaa tgagccagac gcagtcgctg
    60301 aaaaactggc acgccggggc tttaagctgg atgtagataa gctgggcgct cttgaagagc
    60361 gtcgtaaagt attgcaggtc aaaacggaaa acctgcaagc ggagcgtaac tcccgatcga
    60421 aatccattgg ccaggcgaaa gcgcgcgggg aagatatcga gcctttacgt ctggaagtga
    60481 acaaactggg cgaagagctg gatgcagcaa aagccgagct agatgcttta caggctgaaa
    60541 ttcgcgatat cgcgctgacc atccctaacc tgcctgcaga tgaagtgccg gtaggtaaag
    60601 acgaaaatga caacgttgaa gtcagccgct ggggtacccc gcgtgagttt gactttgaag
    60661 ttcgtgacca cgtgacgctg ggtgaaatgc actctggcct cgactttgca gctgcagtta
    60721 agctgactgg ttcccgcttt gtggtaatga aagggcagat tgctcgcatg caccgcgcac
    60781 tgtcgcagtt tatgctggat ctgcataccg aacagcatgg ctacagtgag aactatgttc
    60841 cgtacctggt taaccaggac acgctgtacg gtacgggtca gctgccgaaa tttgctggcg
    60901 atctgttcca tactcgtccg ctggaagaag aagcagacac cagtaactat gcgctgatcc
    60961 caacggcaga agttccgctg actaacctgg tgcgcggtga aatcatcgat gaagatgatc
    61021 tgccaattaa gatgaccgcc cacaccccat gcttccgttc tgaagccggt tcatatggtc
    61081 gtgacacccg tggtctgatc cgtatgcacc agttcgacaa agttgaaatg gtgcagatcg
    61141 tgcgcccaga agactcaatg gcggcgctgg aagagatgac tggtcatgca gaaaaagtcc
    61201 tgcagttgct gggcctgccg taccgtaaaa tcatcctttg cactggcgac atgggctttg
    61261 gcgcttgcaa aacttacgac ctggaagtat ggatcccggc acagaacacc taccgtgaga
    61321 tctcttcctg ctccaacgtt tgggatttcc aggcacgtcg tatgcaggca cgttgccgca
    61381 gcaagtcgga caagaaaacc cgtctggttc ataccctgaa cggttctggt ctggctgttg
    61441 gtcgtacgct ggttgcagta atggaaaact atcagcaggc tgatggtcgt attgaagtac
    61501 cagaagttct gcgtccgtat atgaacggac tggaatatat tggctaatac ccaatttttc
    61561 tgaatctaaa aagcgccttt agggcgcttt ttttgtctcc ctttgatacc gaacaataat
    61621 tactcctcac ttacacgtaa tactactttc gagtgaaaat ctacctatct ctttgatttt
    61681 caaattattc gatgtataca agcctatata gcgagctgct atagaaataa ttacacaata
    61741 cggtttgtta ctggaatcaa tcgtgatcaa gcttgagtga gccattatga aaacgaaaat
    61801 ccctgatgcg gtattggctg ctgaggagag tcgccgtggt ttggtaaaaa cgacagcgat
    61861 cggcggcctg gcaatggcca gcagcgcatt aacattacct tttagtcgga ttgcgcacgc
    61921 tgtcgatagc gccattccaa caaaatcaga cgaaaaggtt atctggagcg cctgtacagt
    61981 taactgtggt agtcgctgcc cgctacgtat gcacgtcgtg gacggtgaaa tcaaatatgt
    62041 cgaaacggac aataccggcg atgacaatta cgacggcctg cactaggttc gcgcctgcct
    62101 gcgtgggcgt tccatgcgtc gccgtgtcta caatccggac cgcctgaaat atccgatgaa
    62161 acgagtcggg gcgcgcggtg aaggcaaatt cgagcgcatt agctgggaag aagcctacga
    62221 catcatcgcg accaatatgc agcgcctgat caaagagtac ggcaacgagt ctatctatct
    62281 gaactatggc accggtacgc tgggcggcac catgacccgc tcctggccgc cgggaaatac
    62341 cctggtcgcg cgcctgatga actgctgcgg cggttatctt aaccattacg gtgactactc
    62401 ttccgcgcaa attgcggaag gtttgaacca tacctacggc ggctgggcag atggtaacag
    62461 cccgtcggat atcgaaaaca gtaaattggt agtgctgttt ggtaataacc caggcgaaac
    62521 gcgaatgagt ggcggtgggg tgacttacta tcttgaacag gcacgccaga aatctaatgc
    62581 tcgcataatc atcatcgatc cgcgctatac cgacaccggt gccgggcgcg aagatgagtg
    62641 gatccctatt cgtccaggaa cagatgccgc actggttaac ggtctggcgt acgtcatgat
    62701 cactgaaaac ctggtggatc aggcattcct cgataaatat tgcgttggct atgacgagaa
    62761 aaccctgccc gccagtgcgc cgaaaaatgg tcattataaa gcttatattc tgggtgaagg
    62821 gccagatggc gtggctaaaa caccggaata ggcctcgcaa atcaccggtg ttccggcaga
    62881 caaaatcatc aaactggctc gtgaaatcgg cagtaccaaa ccggcgttta tcagccaggg
    62941 atggggcccg cagcgtcacg ctaacggtga aatcgcaacc cgtgctatct cgatgctggc
    63001 gattctgacc ggtaacgttg gtattaatgg agggaacagc ggcgcgcgtg aaggttcata
    63061 cagcttaccg tttgtccgta tgccgacctt ggaaaacccg atccagacca gcatttcgat
    63121 gtttatgtgg accgatgcca ttgaacgtgg cccggaaatg acggcgctgc gtgatggtgt
    63181 gcgcgggaaa gataagctgg atgtgccgat caaaatgatc tggaactatg ccggtaactg
    63241 cctgattaac cagcattctg aaatcaaccg tacccatgaa atcctccagg atgataagaa
    63301 gtgcgagctg attgtggtta tcgactgcca catgacctca tcggcgaaat atgctgacat
    63361 cctgctgcct gactgcaccg cttccgaaca gatggacttt gcactggatg catcctgcgg
    63421 gaatatgtct tacgtgattt tcaacgatca ggtgattaaa ccgcgctttg aatgtaagac
    63481 catctatgaa atgaccagcg aactggcaaa acgtcttggc gttgagcaac agtttactga
    63541 aggccgtacc caggaagagt ggatgcggca tctgtatgcc cagtcgcggg aagcgattcc
    63601 tgaactgcca acgtttgaag agttccgcaa gcaggggatc tttaaaaagc gcgacccaca
    63661 agggcatcac gttgcttata aagccttccg tgaagatccg caggcaaacc cactgactac
    63721 gccatcgggc aaaattgaga tttattcgca ggcgctggct gacattgccg ctacctggga
    63781 attgccagaa ggcgatgtga tcgatccact gccgatctac acgccgggct ttgaaagtta
    63841 tcaggatccg ctgaacaaac agtatccgct gcagcttaca ggtttccact ataaatctcg
    63901 cgttcactca acttacggca acgttgatgt gctgaaagcg gcttgccgtc aggaaatgtg
    63961 gatcaacccg cttgatgccc aaaaacgcgg tatccacaac ggcgataaag tcaggatctt
    64021 taacgatcgt ggtgaggttc atattgaggc gaaagtgacg ccacgaatga tgccgggcgt
    64081 ggtcgcactg ggtgaaggcg cctggtatga cccggatgca aaacgtgtcg ataagggcgg
    64141 ttgtatcaac gtactgacca ctcaacgtcc gtctcctctc gctaagggga atccgtcaca
    64201 tacaaacctt gttcaggttg aaaaggtgta aggagtaacc gatgacaacc cagtatggat
    64261 tttttattga ttccagccgt tgcaccggtt gcaaaacctg cgagctggct tgtaaagact
    64321 acaaagattt gacgccagaa gtcagtttcc gccgcattta tgaatatgct ggcggcgact
    64381 ggcaggaaga taacggtgtc tggcaccaga acgtgtttgc ctactatctg tcgatttcgt
    64441 gcaaccactg cgaagatccg gcttgtacta aagtctgccc gagcggtgcg atgcataaac
    64501 gcgaagatgg ttttgttgtg gtcgatgaag atgtatgcat tggctgccgt tactgtcata
    64561 tggcttgccc gtatggcgca ccgcaatata acgaaacgaa aggccatatg accaaatgcg
    64621 atggttgtta tgaccgtgtt gcggagggta aaaagccgat ctgtgttgaa tcttgcccgc
    64681 tgcgggcgct ggatttcggc cctatcgacg agctgcgtaa aaaacatggc gatctggcgg
    64741 ccgttgcgcc gttgccgcga gctcacttta ccaaaccgaa tattgtgatc aaacccaatg
    64801 ccaatagccg cccgacaggg gataccactg gctatctggc aaacccgaag gaggtgtgag
    64861 atgggaagtg gatggcatga atggccgctg atgatcttca cggtcttcgg gcaatgtgtg
    64921 gcgggtggtt ttatcgttct ggctttggcg ctgctcaaag gcgacctgcg agcagaagcc
    64981 cagcagcgtg ttatcgcctg catgtttggc ttatgggtac tgatgggcat tggctttatc
    65041 gcctctatgc tccatcttgg ttcaccgatg cgcgccttta actcgctcaa ccgggtaggg
    65101 gcttcagcac tcagtaacga aatcgccagc ggttcgatct tttttgctgt aggcggcatc
    65161 ggctggttgc tggcaatgct gaaaaagctg tcaccggcat tgcgtacgct atggctggtg
    65221 gtgacgatgg ttcttggcgt cgtctttgtc tggatgatgg tgcgtgtgta taacagcatt
    65281 gataccgttc cgacctggta cagcatctgg acgccgatgg gcttcttcct gacgatgttc
    65341 atgggcggcc cgttactggg ttacctgttg ctgagtctgg ctggcgtcga tggctgggcg
    65401 atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt gatgtcagtg
    65461 atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc agcgctggtg
    65521 ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc cctgtgcttg
    65581 tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc cgtctcattc
    65641 attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt gcatatgacc
    65701 gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc ggttctcttt
    65761 tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc aggaatatgg
    65821 ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg cagctcgcaa
    65881 gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc ccacgccgaa
    65941 tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac atcaaaccct
    66001 tcttcaatgg ctgaaagagc cgggaatgcc acgcaaactt cggttaccac accggcaata
    66061 attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt atcccaggcg
    66121 ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt cagttctaga
    66181 actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg taagttgaaa
    66241 tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg ttcgatatcc
    66301 cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc atcattttta
    66361 tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt ggtacgtctg
    66421 aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg agtttgctgt
    66481 gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc aataaaaaca
    66541 atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat ataaatcgat
    66601 aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt ggcggtggca
    66661 tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt atacccggcc
    66721 tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg tgttaaatac
    66781 actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag gcgttgtcag
    66841 ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg tcattaaacg
    66901 cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg gctgtgccgg
    66961 ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg cgggcgtcgg
    67021 ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga cgtcacgtaa
    67081 ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt ttttaggcca
    67141 gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg ttacaggttt
    67201 gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc aggctgaaaa
    67261 ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc ttggcgtgaa
    67321 tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc ctctgtacct
    67381 caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac tggtcagtgc
    67441 gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc gactgctggt
    67501 tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta gccaggcggc
    67561 gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc cggtggcgat
    67621 ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc aagccttact
    67681 gttgagctat accgtgggaa gtctgcttgg cccgtcattt accgctatgc taatgcagaa
    67741 tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct atttgctgat
    67801 gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa tgaattcaag
    67861 caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct gacttgcttt
    67921 tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt tgcatttcgc
    67981 tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc ccctttaatt
    68041 ttggcgaagg atttgtctat ggctgggaat gttcagga
//
LOCUS       DADMFL010000006        67108 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.006, whole genome shotgun sequence.
ACCESSION   DADMFL010000006 DADMFL010000000
VERSION     DADMFL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.006"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="JNZ27_000381"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="JNZ27_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY8662854.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="JNZ27_000382"
     CDS             complement(1150..1236)
                     /locus_tag="JNZ27_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY8662855.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JNZ27_000383"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JNZ27_000383"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY8662856.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="JNZ27_000384"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="JNZ27_000384"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY8662857.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="JNZ27_000385"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="JNZ27_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY8662858.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="JNZ27_000386"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="JNZ27_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY8662859.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="JNZ27_000387"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="JNZ27_000387"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY8662860.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="JNZ27_000388"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="JNZ27_000388"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY8662861.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="JNZ27_000389"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="JNZ27_000389"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY8662862.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="JNZ27_000390"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="JNZ27_000390"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY8662863.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="JNZ27_000391"
                     /pseudo
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="JNZ27_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="JNZ27_000392"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="JNZ27_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY8662864.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /locus_tag="JNZ27_000393"
     CDS             11798..13453
                     /locus_tag="JNZ27_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY8662865.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRKLEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPTGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="JNZ27_000394"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="JNZ27_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY8662866.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKKPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="JNZ27_000395"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="JNZ27_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY8662867.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="JNZ27_000396"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="JNZ27_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY8662868.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="JNZ27_000397"
     CDS             complement(16982..17746)
                     /locus_tag="JNZ27_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8662869.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="JNZ27_000398"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="JNZ27_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY8662870.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="JNZ27_000399"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="JNZ27_000399"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY8662871.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHAELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="JNZ27_000400"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="JNZ27_000400"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY8662872.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARQIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="JNZ27_000401"
                     /pseudo
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="JNZ27_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="JNZ27_000402"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="JNZ27_000402"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY8662873.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="JNZ27_000403"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="JNZ27_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY8662874.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="JNZ27_000404"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="JNZ27_000404"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY8662875.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="JNZ27_000405"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="JNZ27_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY8662876.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="JNZ27_000406"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="JNZ27_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY8662877.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="JNZ27_000407"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="JNZ27_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY8662878.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="JNZ27_000408"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="JNZ27_000408"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY8662879.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="JNZ27_000409"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="JNZ27_000409"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY8662880.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="JNZ27_000410"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="JNZ27_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY8662881.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="JNZ27_000411"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="JNZ27_000411"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY8662882.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="JNZ27_000412"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="JNZ27_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY8662883.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="JNZ27_000413"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="JNZ27_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY8662884.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="JNZ27_000414"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="JNZ27_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY8662885.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATEIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41828)
                     /locus_tag="JNZ27_000415"
     CDS             complement(40422..41828)
                     /locus_tag="JNZ27_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8662886.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALALALAVLGLIP
                     DMGIWLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTW
                     ALPIFINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQIMGLFV
                     QISLFSESILLTISRVNI"
     gene            complement(41887..42174)
                     /gene="fixX"
                     /locus_tag="JNZ27_000416"
     CDS             complement(41887..42174)
                     /gene="fixX"
                     /locus_tag="JNZ27_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY8662887.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42171..43453)
                     /locus_tag="JNZ27_000417"
                     /pseudo
     CDS             complement(42171..43453)
                     /locus_tag="JNZ27_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(43504..44445)
                     /locus_tag="JNZ27_000418"
                     /pseudo
     CDS             complement(43504..44445)
                     /locus_tag="JNZ27_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44460..45230)
                     /locus_tag="JNZ27_000419"
     CDS             complement(44460..45230)
                     /locus_tag="JNZ27_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY8662888.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45701..47214
                     /gene="caiT"
                     /locus_tag="JNZ27_000420"
                     /pseudo
     CDS             45701..47214
                     /gene="caiT"
                     /locus_tag="JNZ27_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
     gene            47245..48387
                     /gene="caiA"
                     /locus_tag="JNZ27_000421"
     CDS             47245..48387
                     /gene="caiA"
                     /locus_tag="JNZ27_000421"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY8662889.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48516..49733
                     /gene="caiB"
                     /locus_tag="JNZ27_000422"
     CDS             48516..49733
                     /gene="caiB"
                     /locus_tag="JNZ27_000422"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY8662890.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49807..51360
                     /gene="caiC"
                     /locus_tag="JNZ27_000423"
     CDS             49807..51360
                     /gene="caiC"
                     /locus_tag="JNZ27_000423"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY8662891.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51469..52254
                     /gene="caiD"
                     /locus_tag="JNZ27_000424"
     CDS             51469..52254
                     /gene="caiD"
                     /locus_tag="JNZ27_000424"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY8662892.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52260..52850
                     /gene="caiE"
                     /locus_tag="JNZ27_000425"
     CDS             52260..52850
                     /gene="caiE"
                     /locus_tag="JNZ27_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY8662893.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52936..53331)
                     /gene="caiF"
                     /locus_tag="JNZ27_000426"
     CDS             complement(52936..53331)
                     /gene="caiF"
                     /locus_tag="JNZ27_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY8662894.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMLR"
     gene            complement(53593..56814)
                     /gene="carB"
                     /locus_tag="JNZ27_000427"
     CDS             complement(53593..56814)
                     /gene="carB"
                     /locus_tag="JNZ27_000427"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY8662895.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56832..57980)
                     /gene="carA"
                     /locus_tag="JNZ27_000428"
     CDS             complement(56832..57980)
                     /gene="carA"
                     /locus_tag="JNZ27_000428"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY8662896.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58436..59257)
                     /gene="dapB"
                     /locus_tag="JNZ27_000429"
     CDS             complement(58436..59257)
                     /gene="dapB"
                     /locus_tag="JNZ27_000429"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY8662897.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59424..60338)
                     /gene="rihC"
                     /locus_tag="JNZ27_000430"
     CDS             complement(59424..60338)
                     /gene="rihC"
                     /locus_tag="JNZ27_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY8662898.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60404..61354)
                     /gene="ispH"
                     /locus_tag="JNZ27_000431"
                     /gene_synonym="lytB"
     CDS             complement(60404..61354)
                     /gene="ispH"
                     /locus_tag="JNZ27_000431"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY8662899.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61356..61805)
                     /gene="fkpB"
                     /locus_tag="JNZ27_000432"
     CDS             complement(61356..61805)
                     /gene="fkpB"
                     /locus_tag="JNZ27_000432"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8662900.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61892..62386)
                     /gene="lspA"
                     /locus_tag="JNZ27_000433"
     CDS             complement(61892..62386)
                     /gene="lspA"
                     /locus_tag="JNZ27_000433"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY8662901.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62386..65202)
                     /gene="ileS"
                     /locus_tag="JNZ27_000434"
     CDS             complement(62386..65202)
                     /gene="ileS"
                     /locus_tag="JNZ27_000434"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY8662902.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     TDSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGNGEKRKFA"
     gene            complement(65245..66186)
                     /gene="ribF"
                     /locus_tag="JNZ27_000435"
     CDS             complement(65245..66186)
                     /gene="ribF"
                     /locus_tag="JNZ27_000435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY8662903.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66194..66412)
                     /locus_tag="JNZ27_000436"
     CDS             complement(66194..66412)
                     /locus_tag="JNZ27_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY8662904.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66515..66778
                     /gene="rpsT"
                     /locus_tag="JNZ27_000437"
     CDS             66515..66778
                     /gene="rpsT"
                     /locus_tag="JNZ27_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY8662905.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16292 a        18257 c        17266 g        15293 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggcttagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcaacgt
      301 tacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacagaaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa agcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaatgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatgttttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattattt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaat ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaac ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatccta ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatca
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcatctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgta aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca accggtgatc tgatgctaaa aggtccacag ggcgaggaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccgactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaaaccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggcg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccacctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgtctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctttg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt tgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatgc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tgacgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctgcaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggcgatct ggtgcaggtg gtgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gacagatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg actcgtctta tctggcctac
    22321 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttagaa gatctcaaac gctaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgacgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgcctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggttcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacggac
    24661 gcgatgaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cgttatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagccacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 caccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagc ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga caacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tcttgcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cgcccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattt ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattatctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccag cgccagcgcc
    40861 agcgccagcg tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt
    40921 agccacagca tcggcggaat acagccgagc ataaagaaca ggctaatcac cacattccct
    40981 agtgccgcgt ttttgccaac ccccaatccc aacgaaccaa cgatttgcgg gccaaaggtg
    41041 taaatggcga acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa
    41101 ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg
    41161 aaagccaccg gttcgccaaa cagttttatc atcatctctt cgcactcttt tactcgccct
    41221 ttgcgtaata accagcgggg agattctggc agttcgaatc gaccaatcaa aatcaacaaa
    41281 caagggatcg ccgcgctacc cagcatccag cgccaaccac cttccacatc ataaagccag
    41341 tagccgacca gatcggcgca ggtcgcgccg acataccaca tcgcggcaat aaagctgatg
    41401 gaaaacgccc gctgacgggt actggagaac tcggtgatca tcgaagtggc gatgggataa
    41461 tctgcaccga tgacaatgcc gatgagtacc cgcatcacca acagttcgac gggggatgaa
    41521 acacacatcg tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg
    41581 cgccgtccga ctttatcgga gatatagcca aacagcgatg tgccaacgaa cagcccggcg
    41641 agcgttcccg cgcccagcaa gccaatccag tcagcgtcca gtttcagcgc cggagtcagt
    41701 tgctccagcg ccacgccaat cattaccagt acataaccat ccagaaacgg accaccgctt
    41761 ccccacagca aaatgcggcg gtgaatagag gagaatttga gatcgtcaaa gtttctggac
    41821 ggttgcatgg gttattcctg tttttttgca atgagagtca gtacggcgtt acgtatcaaa
    41881 ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc
    41941 cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc
    42001 gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag
    42061 cagctccagc acctgtttat cagcatcggc cttcacaaca atgtgcggat gctcttcatc
    42121 gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg
    42181 gtcactcctt tcatgccatc cttgatcaga ttgatgaagc ccactttctt gccgtggcgg
    42241 aggattttct tgcgcatcag ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc
    42301 acgcccaccg ccagttccgg gtagccgcta aacatgcgtg ggttatcaag gaacgccggt
    42361 agtttctggt acatgcgcat atcgcgcagc gggctgctct ccagatgctg acgatattcc
    42421 gcgagttttt gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca
    42481 gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac
    42541 attccggcgg catcaccggc aatcaatacg ccgtcaccga ccagctccgg cagcatgttg
    42601 atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccgcc cgcgatcagc
    42661 ggtgcaacgg ccggatgctg tttgaaatct tccagcattt gcggcaccga ttttttcgcg
    42721 tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtattttc attggtataa
    42781 aggaagccgc cgcccatcag gccatcggtg ggtgatcccg caaacagaca agccgcgccc
    42841 tgattaccct gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc
    42901 ttcacgccaa ccgccacatc cgtcggttta acgcgttttg ccatccccaa cttttcggca
    42961 aggatggagt tcaccccatc agcaaggagc accgttttcg cttcaatcac atcgccatcg
    43021 gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta
    43081 attaactgcg cgcccgcttc ttcggcctgc tccatcaacc aggcatcaaa tttactgcgc
    43141 aaaacggagt aagaacgctg ggatagcgag gtttcgtcac cgttgcagta gtccatagtc
    43201 atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg
    43261 gcggaatcaa acctggaata atgtgttcca gactgtgggc atagagacgc ccgccagtaa
    43321 cgttcttggc acccgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg
    43381 cgagcaccag tgcggcaacc gaaccggcaa gccctgcacc gacgatgatg gcgtcaaaga
    43441 tatcttcgga cataagagct ccctgtcagc ggcagagccg taatgcagcc ctgccagagt
    43501 ggatcaacgc gctaaagctg cggtcagtgc cggaaggatc ttcacggcat cgccaacaat
    43561 gccgtaatcc gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt
    43621 ttgcgacgcg ttagcgccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta
    43681 cagttcaggt ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt
    43741 ttcgttttct gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg
    43801 ttctgccagt gcaatgttct ctttgctgcc aatgccgcga ccgacgctga ccaccagacg
    43861 ggctttgtcg agatcgacgc tgttgctctg gcgcgcctag gttgccgtgc gggtaatcgc
    43921 cacagccgga gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg
    43981 agccgcatcg aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc
    44041 ttcgccaatc gccagaccgc cgtaaaccat gtgtttcact gttgctttac cgtcctgtac
    44101 gctgacggtg ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc
    44161 cagtaatttg ccgcgacggg tgtttggcaa cagcaccaga ccgtcagcgc cgtgctggcg
    44221 aatagtgtca gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt
    44281 taatttccag acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag
    44341 gacaaaggca ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg
    44401 agagggggta tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat
    44461 taaatgactt tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca
    44521 atcacgatgc gctgacgctc gcgctgtttc ggcgcggcaa cctgttgttc agaccaggcg
    44581 tccacggcgt taaaaccaat atccgccgcc gaccatacct ggactggctt tttcgccgcg
    44641 ccgagaatgg ctttcatcga aggaatttgt ggggagttga tatcagtgga aacagcaaca
    44701 accgcaggca gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg
    44761 agggtatctg ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg
    44821 atttcgccca ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag
    44881 aggatcagat caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc
    44941 ggcagtgcct gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat
    45001 agcacatctt tacgcccttt ggcgttggtc agggctttac cgcccacact taaggctgtc
    45061 acctgcgcct ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttgagatcg
    45121 tattggctta ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc
    45181 gcaatatcct gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca
    45241 gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt
    45301 aatttggaaa taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac
    45361 tttcaatatt gttaagttcc tcaccaatat tgaaaacatg gcgtagcaaa aagaaatttt
    45421 caatattgtt ttatagatca ccaatattga aagtcagatg ctatttttta cccacaaaag
    45481 aacataacaa actgattaat aaccaaaatt aacgaaaaac gtcctctatt cactcgaaac
    45541 aaaaatgtga caccaatcac agaatacagc ttattgaata cccattatga gttagccatt
    45601 aacgcgtcca cgaggttaat aataattata ttaaatgtta acaaaaataa aacaaacggg
    45661 aacgcaaaat aaatattcgt tttcacagtg gaattaactc atgaagaatg aaaagagaaa
    45721 aacgggaata gaaccgaagg ttttctttcc gccattaata atcgtcggca tactttgttg
    45781 gcttacagtc agagatctgg atgcggcgaa tgtcgttatt aatgctgtat tcagttacgt
    45841 caccaatgta tggggatggg catttgaatg gtatatggtg gtgatgcttt tcggttggtt
    45901 ctggctggtg tttggcccgt atgccaaaag cgtttaggta acgaaccgcc agaatttagc
    45961 accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg
    46021 ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga accgaactcg
    46081 acaggagcga aagagttggg gctggcttac agcttgttcc actggggacc tctgccgtgg
    46141 gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg caaaatggaa
    46201 gtgattcgcc ccagctcgac actggtgccg ctggtaggtg aaaaacacgc caaagggttg
    46261 ttcggtacca tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat gggtaccagt
    46321 ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat
    46381 accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc catttgcgtc
    46441 gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc
    46501 ctgatgctgg gttgggtgtt catcgtcagc ggtgccagct tcatcatgaa ctacttcacc
    46561 gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac cgatcccatc
    46621 gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg ggtgatttac
    46681 gccatccaga tgagtatctt cctcgcccgc atcttccgtg gtcgtactgt gcgtgaactg
    46741 tgcttcggca tggtgatggg gctgacagca tccacctgga tcctgtggac cgtgctcggt
    46801 agtaacactc tgctgttgat ggataaaaac atcatcaaca ttccaaatct gatcgaacag
    46861 tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc tgccactcag caccgccacc
    46921 atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa cgcctgctct
    46981 tataccctgg cgatgtctac ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg
    47041 gtgcgtatcg gttggtcaat tctggttggc attatcggta ttgttctgct ggcgctcggc
    47101 ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt cttcgtcaac
    47161 attatggtga cgcactcctt tattaaagac gcgaaacaga actggaaaga ttaattaacc
    47221 cccaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca ggaactgttt
    47281 gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt tgccgagtgc
    47341 gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat gggtatcgac
    47401 agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc
    47461 gtgtggatgg agctgggacg tctgggggca ccaacctatg tactgtacca gctgccgggc
    47521 gggttcaata ccttcctgcg cgaaggcaca caagagcaga tcgacaagat tatggctttc
    47581 cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc gggctccgac
    47641 gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct taatggtagt
    47701 aagtgtttta ttaccagtag cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg
    47761 gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa accgggcatc
    47821 aaagtgacca aacttgagaa gctcggtctg cgtatggata gctgctgtga aatcaccttt
    47881 gacgatgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc
    47941 gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta cggtacggcg
    48001 atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt tggcgaggct
    48061 attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc
    48121 atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat cacctctggc
    48181 gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca
    48241 atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt
    48301 gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa tgcagatcct gacgctgggt
    48361 cgtgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt
    48421 cttatcaggc ctacgggagc acgaatgtaa gccggataag gcgtttacgc cgcatccggc
    48481 agtcatgcgc tacataaatt tttcaggagc taataatgga tcatctaccc atgccgaaat
    48541 tcggaccgct ggccggattg cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg
    48601 ccggacaaat gttcgcagaa tggggcgcgg aagttatttg gatcgagaac gtcgcctggg
    48661 ccgacaccat tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc
    48721 tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca
    48781 ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc attaccgatg
    48841 aagtactgtg gcagcacaac ccgaaactgg ttatcgctca cctgtccggt tttggtcagt
    48901 acggcaccga ggagtacacc aatcttccgg cctataacac catcgcccag gccttcagtg
    48961 gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg tataccgccg
    49021 attacttttc tggcctgacc gccaccacgg cggcgctggc agcactgcat aaagtgcgtg
    49081 aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga agtgatgctg cgtatgggcc
    49141 agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta
    49201 aagatcccta ctacgccggt tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg
    49261 aactggtggg cattacccaa attgaagagt gctttaaaga tattggcctc gcacatctac
    49321 tcggtacgcc ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa tgcccttacg
    49381 gcccactggt agaagaaaaa ctcgatgcct ggctggcggc acataccatc gcagaagtta
    49441 aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta ccggaactgg
    49501 aaagcaatcc acagtatgtt gcccgtgaat caatcactca gtggcaaacg atggatggtc
    49561 gcacctgcaa agggccgaac atcatgccga aattcaaaaa taaccccgga caaatctggc
    49621 gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc ggctacagcg
    49681 aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac taaacgcgtg
    49741 gcgtcagtcg gcttgcctga ctagcgcccg tccaataaca aacgaacaaa aatggataga
    49801 ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa tgtgggacga tctggcggac
    49861 gtttacggtc ataaaacggc gctgatttgt gaatccagcg gcggagtcgt taaccggtat
    49921 agttatcttg agttaaatca ggagattaac cgcacggcaa acctgtttta tacgctgggg
    49981 attcgcaaag gcaacaaggt tgcactacat ctcgacaact gcccggaatt tatcttttgc
    50041 tggttcgggc tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg cctgttacgc
    50101 gaggaaagcg agtggatctt gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa
    50161 ttttatccta tgtatcaaca gattcagcag gaagatgcca ctcaattgcg gcacatttgc
    50221 ctgacagatg tggcacttcc cgctgatgat ggagtgagtt catttactca actgaaaaat
    50281 caacaacctg ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt
    50341 ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg
    50401 cgcttcgctg gatattactc cgcctggcag tgtgcactgc gtgacgatga cgtctacatg
    50461 acggtaatgc ctgcgtttca tatcgattgc cagtgtactg cggcgatggc ggcgttttct
    50521 gccggggcca cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg
    50581 cagaagtacc gcgccaccat taccgaatgt attccgatga tgatccgtac gttgatggtg
    50641 cagccgcctt cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac
    50701 ttgtcggagc aggaaaaaga cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct
    50761 tatgggatga cggaaaccat tgtgggcatt atcggcgatc gccctagcga taaacgacgc
    50821 tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg agatccgcga cgatcacaat
    50881 cgcccgctcc cggcaggtga gatcggtgaa atctgtatta aaggcgtacc agggaaaacc
    50941 atcttcaaag agtattttct caacccgaaa gccactgcga aagtgctgga agccgacggc
    51001 tggctacata ccggcgatac cggataccgc gacgaagaag gcttttttta tttcgtcgat
    51061 cgccgctgca atatgatcaa acgcggtggc gagaatgtct cctgcgtgga gctggaaaat
    51121 atcatcgcca cccatccgaa aattcaggac atcgtggttg tgggtattaa agattcgatt
    51181 cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag
    51241 gaattttttc gcttctgcga acaaaatatg gcgaaattta aagtgccctc ttatctggag
    51301 atcagaaaag atctgccacg taattgctcg gggaaaataa ttagaaagaa tctgaaataa
    51361 atgaaacgtc agggaactac cctgccagct aataattacg ccctaaaaca atatgcattt
    51421 tttgcaggga tgttgtcgtc cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca
    51481 ttacatctga cccgcaatgg atcaattctg gaaattaccc ttgatcgccc aaaagcgaat
    51541 gctattgatg caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat
    51601 ccgcaattac gtgtcgccat tattaccggt gccggagaga aattcttttc cgcgggctgg
    51661 gatttaaaag cggcagcaga aggcgaagca ccggatgctg actttggccc gggtggtttt
    51721 gcgggattaa ccgaaatttt caatctcgac aaaccagtta tcgcagctgt gaacggctac
    51781 gcctttggcg gcggtttcga actggcgctg gcggcagatt ttattgtttg tgccgataac
    51841 gccagcttcg ccctgccgga agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg
    51901 cgtctgccga agatcctgcc gcctaccatc gtcaatgaaa tggtgatgac cggcagacga
    51961 atgggcgcag aagaggcgct gcgttggggg atagtcaacc gcgtggttag ccaggcggaa
    52021 ctgatggata acgcccgcga actggctcag cagctggtta acagcgcccc gctggcgatt
    52081 gcggcgctga aagagatcta ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc
    52141 tatattcgca gcggcgtgtt gaaacactat ccatcggttc tgcattcgga agatgccatt
    52201 gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg
    52261 tgagctatta cgcctttgag gggttaattc cggtggttca cccgacggcg tttgtccatc
    52321 ccagtgccgt cttgattggc gatgtgattg tgggagccgg tgtctacatc ggcccactcg
    52381 cctcactgcg tggtgactac gggcggttga tcgtgcaagc gggagccaat attcaggatg
    52441 gctgcattat gcatggctac tgcgacactg acaccatcgt tggggaaaac ggccatatcg
    52501 ggcatggagc gatcctgcat ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca
    52561 gcgtaattat ggatggcgcg gtcattggcg aagagagcat tgttgccgca atgagctttg
    52621 tcaaagcagg ctttagtggc gagaaacgcc agttgttgat gggtacgcca gcccgcgctg
    52681 tacgcagtgt tagtgacgac gagttacact ggaaacggtt gaataccaaa gagtatcagg
    52741 atcttgtcgg gcgctgtcat gcatcgttac atgaaacgca gccactgagg caaatggagg
    52801 aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc taaacggtga tcttgccgga
    52861 tgcgacctgg ttgctggata agatgctaca acatcgtatc tggcaagcag gacgcccggt
    52921 atcaacgaga taaaattaac gaagcatact ctttgacaac atcatctgcc acttctgctc
    52981 gcggttaagt tccgtgagcg gaacggcagg aatacgcggt gtctttctta cgccgactaa
    53041 tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt
    53101 aaccagtcgc tgacactggc atccccgccc ttccagctta ttagggatca tcttaacttc
    53161 gcagcttatt tctgtgactt ccgacagaat ataagtcagg gtattaaccg ccttgctgtg
    53221 ttcaatatcg aaatgaatag agatctctct tgctatcacc caccgatttt cagccatcat
    53281 ccattcggcg attaacaagt agagtggttt ttcaacatat ccttcacaca tacgaactcc
    53341 agtttcacaa attaaaagtc tggctatgac tcgttttgaa gcaattaaac aaggccatat
    53401 taaaattgtg gatatatcga tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc
    53461 tgcttatgtc atcaaaaata tcgattcccc aaaagattta cttaatgggc aaaaaaaaac
    53521 ggtcctcatc agagaaccgt attagttatt cgatcaaatt agcggatgaa aaatatccgc
    53581 cacgacacgc tattatttga tctgtgcgtg catttcctgc accgaaatta ctttttcagt
    53641 cgcatcggca ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg
    53701 cactttatat tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cacgacggcc
    53761 tgaggtggtg ttgatgatgt aggtatattc gccattcttg atacggtcct gaatgtgcgg
    53821 acggccttca tgcaccttgt ttaccagacg cggattgata cccgcttcgc ccagcacaat
    53881 cgccgtgccg tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc
    53941 caccacgcgt tctttatcgc cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat
    54001 ggtggagttg ctgcccagct gcgctttggc gaacgcttca gcgaaggtgc ggcccacgcc
    54061 catgacttcc ccggtagagc gcatttctgg ccctaacagc gggtcaacgc ctgggaattt
    54121 attgaacggc agcaccactt ctttcaccga gtagtacggc ggaataactt ctttggttac
    54181 gccctgctca gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac
    54241 gccggtggct ttggagacga acggaacagt acgcgccgca cgcgggttta cttcaatcag
    54301 gtaaacttcg ttgtttttca ccgcaaactg cacgttcatc agaccgcgca cctgcaattc
    54361 gaaggccagt ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta
    54421 cgctggcagc gaacatgcgg agtcaccgga gtgcacgccc gcctgctcga tgtgctccat
    54481 gatgccgcca atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctactgc
    54541 gtcatcaagg aaatgatcca gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg
    54601 gaagtagcga cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac
    54661 gtaagacgga cgcaccacca gcgggtagcc aatctctttt gccttctcaa ctgccatttc
    54721 aatagcagta acggtggcgt tcgccggttg tttcagtttc agacggtcaa ccgcatgctg
    54781 gaagcgttca cggtcttccg cacggtcgat agcatccggg ctggtgccga taaccggtac
    54841 gccagcggct tccagtgcac gcgccagttt cagcggggtt tgaccgccgt actgaacgat
    54901 aacgcctttc ggcttctcaa tacgcacgat ttccagcaca tcttccagag ttaccggctc
    54961 gaagtagagg cgatcggaag tgtcgtagtc ggtagagacg gtttccgggt tacagttaac
    55021 cataatggtt tcgtaaccgt cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc
    55081 gaattcgata ccctgaccga tacggtttgg accgccgcca agcaccatga ttttttcacg
    55141 gtcggtagac ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc
    55201 ggtggcaaac tctgccgcac aggtatccac gcgcttgtag accgggtgca ggtcatattg
    55261 gtcacgcagc ttacggattt ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc
    55321 ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac
    55381 ttccgccact ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc
    55441 aatgttagtc aggttgaaga cgccgtccac agacaggccc gcgcggaaag catcggcgat
    55501 gtaccagata cgctctgcgc ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc
    55561 cgggtcatcc aggctcactt tcgggtcgaa gccagttgcg cccacttcca ggccgcgcag
    55621 cgctttttgc agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt
    55681 catctgagtg gtcagacggt cgttagcacc ggcgaatttt tcgaagttga agcgaggaat
    55741 tttagtaacc acatagtcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat
    55801 gtcgttcatc agttcgtcta gggtgtaacc caccgccagt ttcgccgcca ctttagcaat
    55861 cgggaaaccg gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc
    55921 gataacaatc aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt
    55981 ttcaacgccg atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt
    56041 gtcagtcagc gtttgggctg gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc
    56101 atcaaagttt tcgatagagc agacgatgat gcagttgtcg tttttatcac gcaccacttc
    56161 catctcgtac tctttccagc cgatcagcga ctcatcaatc agcaactctt tggtcggaga
    56221 gagatccaga ccgcgggcgc aaatttcttc aaactcttca cggttataag cgataccgcc
    56281 gccgctaccg cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc
    56341 aacagccagc gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat
    56401 tttcttcatc gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcagt
    56461 ggcaccaatc atggtgacac cgaactcttc caacacgccc tgacgttcca gctccagcgc
    56521 gcagttcagc gccgtctgac cgcccatggt tggcagcacc gcgtccgggc gctctttttc
    56581 aataatcttg cgtaccactt cccagtgaat cggctcgatg taggtcgcat cggccatttc
    56641 cgggtcggtc atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc
    56701 gcgcagggct ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac
    56761 aatcgggccc gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct
    56821 tttactcctg attacttagc ggttttacgg tactgctcaa ttaacttgat aaagtggtcg
    56881 aacaacggcg cggcgtcgtg tggaccaggg ctggcttcag ggtgcccctg gaagctgaac
    56941 gccggtttat cgatacgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg
    57001 acacgcaggt ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg
    57061 atcatcacca cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat
    57121 ttcattttga cagtcttcgc accgctcgcc agcgccagca gctgatgacc gagacagatg
    57181 ccgaataccg gaatatcggt ttcgaggaac ttctggatgg cggtaatggc gtaatcgcac
    57241 ggggccgggt cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca
    57301 tcttccgcag aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc
    57361 cgcaggatgt tgcgcttggc accaaaatca taagccacga cgtggaacgg cagctcgtct
    57421 tcttttttcg cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag
    57481 gcttctgcgg tggtcacttc ttttgccaga tccatgccgt tcagacctgg gaacgcgcgg
    57541 gctttttcta acgccagcgc cgcatccggg ttatcgcctg cgataatgca gccattctgt
    57601 gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg
    57661 atgttatggc gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc
    57721 agcggcaggt cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg
    57781 tcattagtgc cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag
    57841 gaaggatcag tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca
    57901 accgccgaac ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc
    57961 aatagcgctg acttaatcaa aacaccctcc atagaatatt cactcacttt atttgcatat
    58021 taattcacag tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac
    58081 ggcggcattc tggagatatg ggcgttaaaa gtcaacttaa tggcaggatt ttttatatta
    58141 ttttgtggct catgcatgtt ttacaggcat taagcgacaa aaacatcatc taacctgttc
    58201 tggaaaacgc ttgcgcagca agaagattgc atgaaataaa aacgagcagg aaaaagtgga
    58261 ccaaatggtc aaaattcaca ttaaaacaac aaaaatagaa gacgaaacat gtaatttttg
    58321 gcagttatag aaaatttaaa agcataacac caaaaataaa agggctaaaa attaaagccc
    58381 tttataatta ataaattaaa tatgttactt ttgcatcata ataaacattt tgtggttaca
    58441 aattattgag atcaagcaca tctcgcatat caaaaagacc actttcctta ccactcaacc
    58501 acaaagccga tcttaccgca ccgttagcaa atgtcatgcg gctggacgcc ttatgggtga
    58561 tctccagacg ctcgccaata tcggcaaaca tcgcggtatg ttcgccaacg atgtcacctg
    58621 cacgcacggt ggcaaaacca atggtaccag gcacacgttc accggtatgg ccttcacgac
    58681 tgtagaccgc gcaatctttc agatctttat caagggcgtg ggcgatcgcc tctcccattg
    58741 ccagtgcggt gcctgacggc gcatcaactt tatgtctatg atgtgcttca ataatttcga
    58801 tatcggtgta gtcacccatc actttggctg ctttctccag cagcttaagc atgacgttaa
    58861 cgccaacgct aaaattggcg gcaaagacaa tcgcaatatc ggcggcggcg tcacgaattg
    58921 cctgtttacc ggcttcgtca aaccccgtag tgccgatcac catccctttg ccatgctgcc
    58981 gacaaaaagc gagatggttc agcgtacctt ccggacgggt aaaatcgata aacacatcaa
    59041 aatcatcttt tatcgcatca agactgcttt gcacggtaac gcctgttttc cctgcaccag
    59101 ccagctcacc ggcgtcgctg cccagtaaag aagatccttc gcgctccagc gccgcgccca
    59161 gctgcacgcc ttctaatgcc agtgccgcct gaatcaactg gcggcccata cgccccccgg
    59221 ctcccgcgat ggcaacgcgg atgtttgcat catgcatagc tattctcttt tgttaatttg
    59281 catagaccgt tttcagagta accagcgcat cgctacgccg ccagccaaaa acgctgataa
    59341 ttagaaatta atgataaaca gggagggata tcagtgaaag gcatgagtcg atgaatgact
    59401 gcatgccgat aacatgtgac aggttacggc accagagcca gcacctcagc cacccactgt
    59461 tggaagcctt ttacattcag atccaatgcc acctgtacat tggctggttt gcccaggcaa
    59521 ccgtcgatat caaccaccgt cgtgcctgag gtaaattcgc cttgagtttc taccgcgaca
    59581 aaacacggct tgagggtgaa taagtccggg cgcaccagcc aggcgatggc gcagagatcg
    59641 tgcattcgca gaccgctttg catactgccg ctacggtagt ggctaaacag ggcgtggagc
    59701 atttttccgg tgcgattcaa ctccggtaaa gtagcgagat agtcaggggt taatattgcc
    59761 tgattggtga catccagacc gcacatgaca atttcgatgc cgctacggaa gacacaggca
    59821 gccgcttctg gatcggcagc gatattaaac tcggcatttg gtgtacagtt gccgcgacca
    59881 gcagaaccgc ccatgatcac cagacgacga atatacggtt tacattccgg gcattgtaaa
    59941 agtaacagcg caatattggt taacgggccg atggccacca gggtaacggg ctctggtgca
    60001 cgcatcaggg catcccgaat cgccagaaac gccggtatcc cgagtggctt tcggttgtgc
    60061 tcaacaaagt cgtagccagc cattcccgat tcgccgtgca cagatgctgc atcacgcggt
    60121 gcgcgtacca gcggcacagc ggctccctgg gcgagcggaa tctccgcatt ccagaaatgc
    60181 agcagttgca gggcattgcg ggtagttttc tcaaccgaga cattacccgc gacggtggtc
    60241 atcagttgca ggtcgagttc gggggcaaaa atcgcggcgg caatggcgac ggcatcgtca
    60301 atgccggggt cagtatcgag gaagataggt aaacgcatgt tttcttcata aaaaatgccg
    60361 gtaacaagac cggcattttc gcataactta ggctgctaat gacttaatcg acttcacgaa
    60421 tatcgacacg cagctctttc ggcacttcga aaacaatatt ttcttcacgg ccttccagcg
    60481 gaatggcttc accaccaccc aactgctgca aacgtgccac cacattctgc accagaatat
    60541 ccggagccga tgcgcccgca gtcacgccga cgcatttaac ctctttcacc cactcttcct
    60601 ggatatcttt cgcatcgtca atcaaaaacg cgtgtttgcc catacgctgg gccagctccg
    60661 ccagacggtt ggagttggag gagtttttcg aaccgaccac caacacaact tccgcctgtt
    60721 ctgccagggc gcgtaccgct tcctgacggt tagtcgtggc gtagcagatg tcatctttgc
    60781 gcggaccgac aattttcggg aagcgtttac gcagcgcatc gatcacatca gacgtatcat
    60841 ccaccgacag cgtggtttgg gtcataaagg agagcttctc ttcgtttttg accgtcagtt
    60901 tccacacatc gtctggcgat tcgaccagat acattccccc ttcggggttg ctgtactgcc
    60961 ccatcgtccc ttccacttcc gggtgcccgg cgtggccgat gagaatagat tcttcgccac
    61021 ggcgactggc gcgggcaact tccatatgca ctttggtcac cagcggacag gtggcatcaa
    61081 acaccgtcaa atcgcggctt ttcgcttcgt tacgaaccgc ctgagaaaca ccgtgtgcgg
    61141 agaaaatcag gatcgcgccg tccggcactt cgctaatctg ctcaataaag atagccccac
    61201 gctcgcgcaa gctatcgacc acgtagcggt tatgcaccac ttcgtgacgg acatatatcg
    61261 gtgcgccgta aatggccagc gcgttttcaa caatgctgat agcgcggtct accccggcac
    61321 aaaaaccacg cgggttggcc aacaggatct gcatgttacg cctccagtgc cggatcgatt
    61381 tccagcactt caatatcaaa atgaacggtc tgcccggcca gcggatggtt gaaatcaacg
    61441 gtaatggagt cgccgttaat ttcgcggatc acaccaggca tctcactgcc atccattgcg
    61501 gtaaaaagca tgattgcgcc aatttctggc tcgcctgcat ccataaattc acggcgggag
    61561 aagtactgaa tcaggtccgg tgacggcacg ccaaacgccg catctggctc cagcgagaag
    61621 gtggttttgt cgcccacttt cagccccagc aggtgttgct ccagcccttc agaaagagaa
    61681 gcatcgccca ggcggaacag cgccggttta ccgttgttgc gagtagactc ggcggtggtg
    61741 ccatcgtcga gttttagcgt gaagtgcacc aggacggcgc tattgctctg tacagattca
    61801 gacatgcagg tttgctctta tttttgagga tttttgccgg atgcggccta cgatttcgta
    61861 gcaatctgta ggccgcatcc ggcagggttt attattgttt tttcgctttg gaaggtaaaa
    61921 acccttccag cacgatcagt gccgcaccga cacaaatggc agtatcggca aggttgaagg
    61981 tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag ccgtgccaca
    62041 ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg atattgttta
    62101 gcttctgcgt ggccttcgag cgatacatca tcactgccag gagcacgcta ataccaatcg
    62161 caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa ctaaacgccg
    62221 cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc gtatccccca
    62281 gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc acgactacca
    62341 ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg caaacttacg
    62401 tttttcaccg ttaccggcga cgttgctgac acagcggccg cagatttctg cgtgttccgc
    62461 caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac cttcggcttt
    62521 actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag caggtgcgtc
    62581 gttatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt catcgcccag
    62641 cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg cttccagcga
    62701 gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt cgccacgcac
    62761 tttcaacagc tcgtcccaga acgcatcgtt cattgcttca ctgtctgtca gaccaaacag
    62821 gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca gatagcccca
    62881 cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg cttcagcgat
    62941 gtgatacagc gcagtctggc agctacgacg cgccacgcta tccgctttgg cggtgtactg
    63001 acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga agcgcatcag
    63061 acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt cttcctgtgc
    63121 cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg gtttcaccat
    63181 atcttttgct ggatcaaaac cattcaggtt tgccagcagg aagcgcgcgg tgttacggat
    63241 acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca tttcaccggt
    63301 gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt tcatcacatc
    63361 ctgcggcgaa acggtgttgc cgatggattt agacatcttg cgaccctgac catccacggt
    63421 aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg tggagatcat
    63481 tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca tgtccgctgc
    63541 gtgaccggca aattccggac gcacgtcaac aacagaagag tgggtagatc cggagtcaaa
    63601 ccatacgtcg agagtatccg gcactttcac atactggtca gcttcgtcgc cgaggatctc
    63661 tttcgcatcg agatcccacc acgcctggat gccgtcaact tcaacgcgtt tagccacttc
    63721 ttccatcagt tcgagggtac gcggatgcag ctcttccgtg tctttgtgca cgaacagtga
    63781 catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt tagcaaccat
    63841 cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga tctctttcag
    63901 tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggtg tcgcgcggaa
    63961 gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt tctcaacgtg
    64021 cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt tgaagacgtt
    64081 cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc caaccgggtt
    64141 agcggtttcc aagccgtatt tctgaccgat cacatagtcg tccgggccgt ggccaggcgc
    64201 ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga ttgccggaac
    64261 gtcgaagccc ataaacggat gggtaaagcg cagcagctca agctctgcac cttttaccgt
    64321 gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa ccagatcttt
    64381 cgccagaatc gcggcctgac cgtcgatctg caccagcgca tagtcgaaat caggtgcaat
    64441 agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca ccagcgagat
    64501 tgggccgtta acgttgctta cgccaaattt cgctttcagc gcatcctgat cgaccgcctg
    64561 gaaagcgacg tcaatggacg gagaagtttt gtcgtaatac tcaacttccg cttctgccag
    64621 tgcagaacgg cagtctacgc accagtgcac cggcttcgcg cctttgtgca ggtgaccatt
    64681 gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt ccatagtcag
    64741 atacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc gttgaccgtc
    64801 aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg tgaatttctc
    64861 acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt ggcagtccca
    64921 gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga taatgtcttt
    64981 cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag gagggccatc
    65041 atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt acagatcatc
    65101 atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac gcatcgggaa
    65161 ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct cggttccgtg
    65221 tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa attcgcgggc
    65281 ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa atcgctgctc
    65341 attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt ccattgcaac
    65401 atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc gtgttccgat
    65461 gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctaccg cataaacccc
    65521 tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac ctatagtgcg
    65581 ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc ccagtaaact
    65641 ttctgccaga gccagattgt catcagccag cgcctgacgc acggcggtgc tgctgatacg
    65701 cacaccacct tcgcaaaaag tttgcgtact ggtgatatcg aagccgtatt ccatgccagc
    65761 tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat catcacctac
    65821 ggcaagaaat tttacgcgca aatgcttcac caaaagatcg ctgatgaaat tttgcgcggt
    65881 taacgccgca aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc cacactctgc
    65941 aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat cggtagcaaa
    66001 cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc gcttgcgccc
    66061 ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt cgaaattacc
    66121 aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc cgcgtatcag
    66181 cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca gcggtcaagg
    66241 ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc atggcgcaaa
    66301 tcacgggaag aaactgaccg cctgctgcaa tttttatcgc ggaaaagctg tattcacacc
    66361 ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac attaacggcg
    66421 cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct cggcctttga
    66481 attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat cagctaagaa
    66541 gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg
    66601 tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg ctgcacagaa
    66661 agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc tgatccacaa
    66721 aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac tggcttaatc
    66781 gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta tgcctgctgc
    66841 ttttgtgatg cgtgagagaa cacactgccg gttgccagat agtcaaaaga gacatgccgt
    66901 tgtactatct gaactcttcg tggcttaaga atcgtagaac catcggctga tacattctga
    66961 acaagaatat ctactcaatc tccagactcc cccacaaaga atatggatat tgtgatacac
    67021 attgaggtat taaacttaag accactctat ttttgaatta aattcctata tactttaacc
    67081 attcataaat atctcctcat tagacctt
//
LOCUS       DADMFL010000007        65615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.007, whole genome shotgun sequence.
ACCESSION   DADMFL010000007 DADMFL010000000
VERSION     DADMFL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.007"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JNZ27_000438"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JNZ27_000438"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY8662906.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JNZ27_000439"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JNZ27_000439"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY8662907.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JNZ27_000440"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JNZ27_000440"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY8662908.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JNZ27_000441"
     CDS             complement(3025..3480)
                     /locus_tag="JNZ27_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY8662909.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JNZ27_000442"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JNZ27_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY8662910.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYNAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JNZ27_000443"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JNZ27_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY8662911.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JNZ27_000444"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JNZ27_000444"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY8662912.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTEEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="JNZ27_000445"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="JNZ27_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY8662913.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="JNZ27_000446"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="JNZ27_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY8662914.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FIWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="JNZ27_000447"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="JNZ27_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY8662915.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="JNZ27_000448"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="JNZ27_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY8662916.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="JNZ27_000449"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="JNZ27_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY8662917.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="JNZ27_000450"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="JNZ27_000450"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY8662918.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="JNZ27_000451"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="JNZ27_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY8662919.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="JNZ27_000452"
     CDS             19305..19370
                     /locus_tag="JNZ27_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8662920.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="JNZ27_000453"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="JNZ27_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY8662921.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="JNZ27_000454"
     CDS             complement(19754..20377)
                     /locus_tag="JNZ27_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY8662922.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="JNZ27_000455"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="JNZ27_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY8662923.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="JNZ27_000456"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="JNZ27_000456"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY8662924.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="JNZ27_000457"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="JNZ27_000457"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY8662925.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="JNZ27_000458"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="JNZ27_000458"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY8662926.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /locus_tag="JNZ27_000459"
                     /pseudo
     CDS             25216..26372
                     /locus_tag="JNZ27_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26461..26847)
                     /locus_tag="JNZ27_000460"
     CDS             complement(26461..26847)
                     /locus_tag="JNZ27_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY8662927.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="JNZ27_000461"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="JNZ27_000461"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY8662928.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="JNZ27_000462"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="JNZ27_000462"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY8662929.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGCLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="JNZ27_000463"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="JNZ27_000463"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY8662930.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DSFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="JNZ27_000464"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="JNZ27_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY8662931.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="JNZ27_000465"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="JNZ27_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY8662932.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="JNZ27_000466"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="JNZ27_000466"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY8662933.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="JNZ27_000467"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="JNZ27_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY8662934.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="JNZ27_000468"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="JNZ27_000468"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY8662935.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="JNZ27_000469"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="JNZ27_000469"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY8662936.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="JNZ27_000470"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="JNZ27_000470"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY8662937.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="JNZ27_000471"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="JNZ27_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY8662938.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="JNZ27_000472"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="JNZ27_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY8662939.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="JNZ27_000473"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="JNZ27_000473"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY8662940.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="JNZ27_000474"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="JNZ27_000474"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY8662941.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="JNZ27_000475"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="JNZ27_000475"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY8662942.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="JNZ27_000476"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="JNZ27_000476"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY8662943.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPSCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="JNZ27_000477"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="JNZ27_000477"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY8662944.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="JNZ27_000478"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="JNZ27_000478"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY8662945.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..50646
                     /gene="dnaE"
                     /locus_tag="JNZ27_000479"
     CDS             47164..50646
                     /gene="dnaE"
                     /locus_tag="JNZ27_000479"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY8662946.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50659..51618
                     /gene="accA"
                     /locus_tag="JNZ27_000480"
     CDS             50659..51618
                     /gene="accA"
                     /locus_tag="JNZ27_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY8662947.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51717..53858
                     /gene="ldcC"
                     /locus_tag="JNZ27_000481"
     CDS             51717..53858
                     /gene="ldcC"
                     /locus_tag="JNZ27_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY8662948.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53915..54304
                     /locus_tag="JNZ27_000482"
     CDS             53915..54304
                     /locus_tag="JNZ27_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8662949.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54369..55667
                     /gene="tilS"
                     /locus_tag="JNZ27_000483"
     CDS             54369..55667
                     /gene="tilS"
                     /locus_tag="JNZ27_000483"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY8662950.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55716..55976)
                     /gene="rof"
                     /locus_tag="JNZ27_000484"
     CDS             complement(55716..55976)
                     /gene="rof"
                     /locus_tag="JNZ27_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY8662951.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55963..56163)
                     /locus_tag="JNZ27_000485"
     CDS             complement(55963..56163)
                     /locus_tag="JNZ27_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY8662952.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56329..56874
                     /locus_tag="JNZ27_000486"
     CDS             56329..56874
                     /locus_tag="JNZ27_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY8662953.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56871..57293
                     /gene="arfB"
                     /locus_tag="JNZ27_000487"
     CDS             56871..57293
                     /gene="arfB"
                     /locus_tag="JNZ27_000487"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8662954.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQNVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57307..58017
                     /gene="nlpE"
                     /locus_tag="JNZ27_000488"
                     /gene_synonym="cutF"
     CDS             57307..58017
                     /gene="nlpE"
                     /locus_tag="JNZ27_000488"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY8662955.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58172..58996)
                     /locus_tag="JNZ27_000489"
     CDS             complement(58172..58996)
                     /locus_tag="JNZ27_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY8662956.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59049..60767)
                     /gene="proS"
                     /locus_tag="JNZ27_000490"
     CDS             complement(59049..60767)
                     /gene="proS"
                     /locus_tag="JNZ27_000490"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY8662957.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="JNZ27_000491"
     CDS             complement(60878..61585)
                     /gene="tsaA"
                     /locus_tag="JNZ27_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY8662958.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="JNZ27_000492"
     CDS             complement(61582..61986)
                     /gene="rcsF"
                     /locus_tag="JNZ27_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY8662959.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="JNZ27_000493"
     CDS             complement(62104..62919)
                     /gene="metQ"
                     /locus_tag="JNZ27_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY8662960.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62959..63612)
                     /gene="metI"
                     /locus_tag="JNZ27_000494"
     CDS             complement(62959..63612)
                     /gene="metI"
                     /locus_tag="JNZ27_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY8662961.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63605..64636)
                     /gene="metN"
                     /locus_tag="JNZ27_000495"
     CDS             complement(63605..64636)
                     /gene="metN"
                     /locus_tag="JNZ27_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY8662962.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64824..65399
                     /gene="gmhB"
                     /locus_tag="JNZ27_000496"
     CDS             64824..65399
                     /gene="gmhB"
                     /locus_tag="JNZ27_000496"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY8662963.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKCTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15513 a        16251 c        18083 g        15768 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgcgttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg cacagttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggaggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagttggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt atcattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg gcagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accatctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgctcaa tctgtacaat ccggtgaatc ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttatct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtttgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgattct cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc ggcggtgatg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga taatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acatatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gcttcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac gccgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttatcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggt gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcggtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggggcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggagatttc gcgggccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgtcg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccggttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtat
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacgggca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctcggc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggtaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctagcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggtgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gcctttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagcgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagcccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaataccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagcagcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaactatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catctttggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat
    34441 gtacgagcaa ttgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctgggctgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttacgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgagtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcaatgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgaag cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagtggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgattt ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctaaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccgggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga ccttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca
    40861 ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 atactgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcactt
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ctgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caagcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43381 gagtccggcg ttgaactggg ggataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc ttcttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctagcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg tttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtagga
    48241 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatccg
    49501 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49561 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49621 ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgccatcaaa
    49681 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49741 cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49801 gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg
    49861 aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49921 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc
    49981 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    50041 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    50101 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacagc tgcggggctc
    50161 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50221 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50281 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50341 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50401 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50461 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50521 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50581 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50641 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50701 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50761 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc
    50821 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca gcgtccttat
    50881 accctagatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgtgcg
    50941 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg
    51001 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    51061 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    51121 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51181 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct tggcgtaccg
    51241 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51301 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51361 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51421 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51481 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51541 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51601 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51661 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51721 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51781 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51841 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51901 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51961 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    52021 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacacca
    52081 acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc tacgtcaaag
    52141 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52201 cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct gatgtctcta
    52261 tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg gaagcggaag
    52321 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaacatcga
    52381 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52441 gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52501 tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt gaatttactc
    52561 gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg gttcatgcgg
    52621 tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc aaacagacgc
    52681 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat tttcatccaa
    52741 tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt ttcttcgaaa
    52801 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc cacattaaag
    52861 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52921 gttatcccat tgttgcttcg gttgagacgg cggcggcgat gctgcgtggt aatccgggca
    52981 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggc
    53041 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    53101 ccgaatgctg gcccgttgcg cctggcgaac aatggcacgg ctttaacgat gcggatgccg
    53161 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53221 gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc gacgaacgtg
    53281 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggcatcg
    53341 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc tcttacgatc
    53401 tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc gatttctacc
    53461 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53521 tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53581 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53641 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53701 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53761 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53821 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53881 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53941 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atattcttgg
    54001 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    54061 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    54121 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54181 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54241 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54301 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54361 gcaccatcat gacactcacg ctcaatagac cacttctcac ctcacgccag attctggtgg
    54421 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54481 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54541 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54601 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg caggcgcgtt
    54661 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54721 acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54781 cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg ttgcttgccc
    54841 gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54901 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54961 tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca ctttgtgctg
    55021 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    55081 aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc gcggcgatta
    55141 ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgca
    55201 tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt ttgggcgcct
    55261 ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc gggcaaagcg
    55321 aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55381 ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55441 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga cgtaagctga
    55501 agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55561 tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55621 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55681 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac caccaccgta
    55741 ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg ggtttcgcca
    55801 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact ggcttttgcc
    55861 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55921 tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat tgacataatc
    55981 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    56041 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    56101 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    56161 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56221 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56281 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56341 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56401 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56461 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56521 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56581 gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga agtggcgctg
    56641 tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg
    56701 cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc
    56761 tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat ctggttatcg
    56821 gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga
    56881 tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg
    56941 gcgctggcgg gcagaatgtt aataagacct caacggctat tcatctgcgt tttgacattc
    57001 gggcgcccag cctgccagag tattacaaag agcgtctgct cgccgccagc catcatttga
    57061 tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc
    57121 gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa gaacaaaaag
    57181 cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac
    57241 aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag
    57301 gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg
    57361 atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc tgccgaactg
    57421 aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga aggaatcgaa
    57481 atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct
    57541 cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg
    57601 gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag
    57661 atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct ggaaccggca
    57721 caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta tatggctgat
    57781 gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag
    57841 ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta
    57901 gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa agtattagcg
    57961 cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg gcagtaaacc
    58021 tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca ggcctacagc
    58081 atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc
    58141 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
    58201 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58261 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58321 gcgccagtgt aagtggatgc ccggctttgg caaatgcatc agtgacaaat tccgaacaaa
    58381 accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc aggtcgctga
    58441 cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
    58501 taaagggaat aaattcgaca atactgcgat agttataacc gctatctttg attttattgg
    58561 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
    58621 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
    58681 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
    58741 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
    58801 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
    58861 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
    58921 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg cggcagtacg
    58981 cctttggttt atccatttta tacaatctat gtaaaaaagg gccctgaaat tcaggaccct
    59041 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
    59101 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
    59161 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
    59221 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
    59281 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
    59341 acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta gttctgctca
    59401 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
    59461 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
    59521 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
    59581 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
    59641 tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
    59701 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
    59761 cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
    59821 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
    59881 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
    59941 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
    60001 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
    60061 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
    60121 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggcc
    60181 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
    60241 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
    60301 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
    60361 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
    60421 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
    60481 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcggct
    60541 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
    60601 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
    60661 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
    60721 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
    60781 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga tttagtttac
    60841 cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc agcgcaaaga
    60901 cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc cagacggcat
    60961 aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc gccagtactt
    61021 cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc tgcttttcga
    61081 cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca tagctggcac
    61141 tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact accggcgtac
    61201 catcgaccag atccagactg ccgagcttca gaatcacgct gtctttatgg caaacaacct
    61261 ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag cgtgttgcga
    61321 aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga cgccagccgc
    61381 cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct tccaggccgc
    61441 gaacggcgtc ggcctggttg taggaagcaa tgagatgcag ttcgccgttg gcgcttttta
    61501 ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga ataacgccta
    61561 tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc cgatacatac
    61621 agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca gtaatacagc
    61681 attggctttc attttagagg cattgatttg catctgctta cgtgcggttg gaatgctcgg
    61741 cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga gatcgcggaa
    61801 cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc
    61861 tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg
    61921 ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga tcggtaaagc
    61981 acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg agcttgtaaa
    62041 aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga aacagcctaa
    62101 aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt cgtaaacttc
    62161 atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt tatcttcacg
    62221 cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac cgtctttcgc
    62281 cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag cgatttgcgc
    62341 gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca gattttttgg
    62401 gttctcaaca acatcaagaa ctgtcggcag taggccaacg ccatctttca gtttgatcaa
    62461 gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt ttggcacggc
    62521 aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt aaccagcaat
    62581 cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt tcagttgctg
    62641 atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc
    62701 gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat atttgtcttt
    62761 cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca cgccgacttt
    62821 aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg atccaatcag
    62881 ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct tcttcaatta
    62941 tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt cgcctgcgaa
    63001 ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt
    63061 gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac cgactgcacc
    63121 acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat tcaccagacc
    63181 cggcaacgct tccggtaaca gcactttacg gacgatctgc attggcgtgg cacccattgc
    63241 gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga ccatacgggc
    63301 aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac caatcgatgt
    63361 accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga acgggatgga
    63421 acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag caataatttg
    63481 ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa acccaaaaaa
    63541 tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca gccacatcat
    63601 cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc caggcaatgg
    63661 cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg aatttaacgc
    63721 caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg
    63781 tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc agcatcggca
    63841 cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg atatccagat
    63901 gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag aacacttcac
    63961 ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa tcacaaatac
    64021 gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga cggcggttaa
    64081 tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg ctggtggctt
    64141 catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc acacgttgtt
    64201 tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga ccaaccaatg
    64261 acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc agctccagcg
    64321 gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc tggaaaatca
    64381 taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc aattcctggc
    64441 catcgaccag cacgctaccc tcggttgggc gctccagcag gtttacacaa cgtataagcg
    64501 tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct ggcacatgca
    64561 ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact ttggtgatat
    64621 tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag catctcgaat
    64681 gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac gtctaaatca
    64741 atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact agcgtcccat
    64801 gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt cttgaccgtg
    64861 atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt gaatttatcg
    64921 acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa
    64981 ccaaccagtc tggcattgct cgcggtaaat gtaccgaagc acagtttgaa acgctgaccg
    65041 agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc tattattgcc
    65101 cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc cgcaaaccac
    65161 atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc gcttcttata
    65221 tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg ggaacaaaag
    65281 tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca gattgggtgt
    65341 taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg gcgcaatgat
    65401 taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg agccgactcc
    65461 ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac agtcggttcg
    65521 gttgaagaga aaaaatcctg aaattcaggg ttgactctga aagaggaaag tgtgatatac
    65581 gccacctcgc gacagtgagc tgaaagctgc gtcgc
//
LOCUS       DADMFL010000008        65048 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.008, whole genome shotgun sequence.
ACCESSION   DADMFL010000008 DADMFL010000000
VERSION     DADMFL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.008"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNZ27_000497"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNZ27_000497"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY8662964.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNZ27_000498"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNZ27_000498"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY8662965.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNZ27_000499"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNZ27_000499"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY8662966.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JNZ27_000500"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JNZ27_000500"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY8662967.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JNZ27_000501"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JNZ27_000501"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY8662968.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JNZ27_000502"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JNZ27_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY8662969.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JNZ27_000503"
     CDS             9708..10559
                     /locus_tag="JNZ27_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8662970.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRQQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JNZ27_000504"
     CDS             complement(10546..10887)
                     /locus_tag="JNZ27_000504"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8662971.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JNZ27_000505"
     CDS             complement(10889..11767)
                     /locus_tag="JNZ27_000505"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY8662972.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JNZ27_000506"
     CDS             complement(11733..14030)
                     /locus_tag="JNZ27_000506"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8662973.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JNZ27_000507"
     CDS             complement(14081..14401)
                     /locus_tag="JNZ27_000507"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8662974.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15471)
                     /locus_tag="JNZ27_000508"
     CDS             complement(14416..15471)
                     /locus_tag="JNZ27_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY8662975.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAIAAVGICIP
                     PLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVIPSIMVGSV
                     CGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVLKSLARKNG
                     SSTDEKEDDLDLDFEIN"
     gene            15780..18281
                     /gene="ptsP"
                     /locus_tag="JNZ27_000509"
     CDS             15780..18281
                     /gene="ptsP"
                     /locus_tag="JNZ27_000509"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8662976.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18293..18955
                     /gene="fsa"
                     /locus_tag="JNZ27_000510"
     CDS             18293..18955
                     /gene="fsa"
                     /locus_tag="JNZ27_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8662977.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18966..20058
                     /gene="gldA"
                     /locus_tag="JNZ27_000511"
                     /pseudo
     CDS             18966..20058
                     /gene="gldA"
                     /locus_tag="JNZ27_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20333..20950
                     /locus_tag="JNZ27_000512"
     CDS             20333..20950
                     /locus_tag="JNZ27_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY8662978.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20977..21915)
                     /gene="yijE"
                     /locus_tag="JNZ27_000513"
     CDS             complement(20977..21915)
                     /gene="yijE"
                     /locus_tag="JNZ27_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY8662979.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21976..24156)
                     /gene="katG"
                     /locus_tag="JNZ27_000514"
     CDS             complement(21976..24156)
                     /gene="katG"
                     /locus_tag="JNZ27_000514"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY8662980.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24485..25375)
                     /gene="metF"
                     /locus_tag="JNZ27_000515"
     CDS             complement(24485..25375)
                     /gene="metF"
                     /locus_tag="JNZ27_000515"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY8662981.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25724..28156)
                     /locus_tag="JNZ27_000516"
     CDS             complement(25724..28156)
                     /locus_tag="JNZ27_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY8662982.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28159..29319)
                     /gene="metB"
                     /locus_tag="JNZ27_000517"
     CDS             complement(28159..29319)
                     /gene="metB"
                     /locus_tag="JNZ27_000517"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY8662983.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29596..29913
                     /gene="metJ"
                     /locus_tag="JNZ27_000518"
     CDS             29596..29913
                     /gene="metJ"
                     /locus_tag="JNZ27_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY8662984.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30097..30705
                     /locus_tag="JNZ27_000519"
     CDS             30097..30705
                     /locus_tag="JNZ27_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY8662985.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30766..30978)
                     /gene="rpmE"
                     /locus_tag="JNZ27_000520"
     CDS             complement(30766..30978)
                     /gene="rpmE"
                     /locus_tag="JNZ27_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY8662986.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31181..33379
                     /gene="priA"
                     /locus_tag="JNZ27_000521"
     CDS             31181..33379
                     /gene="priA"
                     /locus_tag="JNZ27_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY8662987.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33535..34560
                     /gene="cytR"
                     /locus_tag="JNZ27_000522"
     CDS             33535..34560
                     /gene="cytR"
                     /locus_tag="JNZ27_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY8662988.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34652..35611
                     /gene="ftsN"
                     /locus_tag="JNZ27_000523"
     CDS             34652..35611
                     /gene="ftsN"
                     /locus_tag="JNZ27_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY8662989.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35704..36234
                     /gene="hslV"
                     /locus_tag="JNZ27_000524"
     CDS             35704..36234
                     /gene="hslV"
                     /locus_tag="JNZ27_000524"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY8662990.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36244..37575
                     /gene="hslU"
                     /locus_tag="JNZ27_000525"
     CDS             36244..37575
                     /gene="hslU"
                     /locus_tag="JNZ27_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY8662991.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37642..38568
                     /gene="menA"
                     /locus_tag="JNZ27_000526"
     CDS             37642..38568
                     /gene="menA"
                     /locus_tag="JNZ27_000526"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY8662992.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38661..39146
                     /gene="rraA"
                     /locus_tag="JNZ27_000527"
     CDS             38661..39146
                     /gene="rraA"
                     /locus_tag="JNZ27_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY8662993.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39231..39476)
                     /gene="zapB"
                     /locus_tag="JNZ27_000528"
     CDS             complement(39231..39476)
                     /gene="zapB"
                     /locus_tag="JNZ27_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY8662994.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39902..40747
                     /gene="glpF"
                     /locus_tag="JNZ27_000529"
                     /pseudo
     CDS             39902..40747
                     /gene="glpF"
                     /locus_tag="JNZ27_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40787..42277
                     /gene="glpK"
                     /locus_tag="JNZ27_000530"
     CDS             40787..42277
                     /gene="glpK"
                     /locus_tag="JNZ27_000530"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY8662995.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42507..43517
                     /gene="glpX"
                     /locus_tag="JNZ27_000531"
     CDS             42507..43517
                     /gene="glpX"
                     /locus_tag="JNZ27_000531"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8662996.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43614..44360
                     /gene="fpr"
                     /locus_tag="JNZ27_000532"
     CDS             43614..44360
                     /gene="fpr"
                     /locus_tag="JNZ27_000532"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY8662997.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44365..44793)
                     /gene="uspD"
                     /locus_tag="JNZ27_000533"
     CDS             complement(44365..44793)
                     /gene="uspD"
                     /locus_tag="JNZ27_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY8662998.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWAKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44820..45119)
                     /locus_tag="JNZ27_000534"
     CDS             complement(44820..45119)
                     /locus_tag="JNZ27_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY8662999.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45331..45771)
                     /locus_tag="JNZ27_000535"
                     /pseudo
     CDS             complement(45331..45771)
                     /locus_tag="JNZ27_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45872..46471
                     /locus_tag="JNZ27_000536"
     CDS             45872..46471
                     /locus_tag="JNZ27_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY8663000.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46579..47346
                     /gene="tpiA"
                     /locus_tag="JNZ27_000537"
     CDS             46579..47346
                     /gene="tpiA"
                     /locus_tag="JNZ27_000537"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY8663001.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47401..48156)
                     /gene="cdh"
                     /locus_tag="JNZ27_000538"
     CDS             complement(47401..48156)
                     /gene="cdh"
                     /locus_tag="JNZ27_000538"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY8663002.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48263..49252)
                     /locus_tag="JNZ27_000539"
     CDS             complement(48263..49252)
                     /locus_tag="JNZ27_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8663003.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49572..50534)
                     /gene="pfkA"
                     /locus_tag="JNZ27_000540"
     CDS             complement(49572..50534)
                     /gene="pfkA"
                     /locus_tag="JNZ27_000540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY8663004.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50715..51617)
                     /gene="fieF"
                     /locus_tag="JNZ27_000541"
     CDS             complement(50715..51617)
                     /gene="fieF"
                     /locus_tag="JNZ27_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY8663005.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51765..52265)
                     /gene="cpxP"
                     /locus_tag="JNZ27_000542"
     CDS             complement(51765..52265)
                     /gene="cpxP"
                     /locus_tag="JNZ27_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY8663006.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52415..53113
                     /gene="cpxR"
                     /locus_tag="JNZ27_000543"
     CDS             52415..53113
                     /gene="cpxR"
                     /locus_tag="JNZ27_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY8663007.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53110..54483
                     /gene="cpxA"
                     /locus_tag="JNZ27_000544"
     CDS             53110..54483
                     /gene="cpxA"
                     /locus_tag="JNZ27_000544"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY8663008.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54589..55263)
                     /gene="yiiM"
                     /locus_tag="JNZ27_000545"
     CDS             complement(54589..55263)
                     /gene="yiiM"
                     /locus_tag="JNZ27_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY8663009.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55412..56395)
                     /gene="kdgT"
                     /locus_tag="JNZ27_000546"
     CDS             complement(55412..56395)
                     /gene="kdgT"
                     /locus_tag="JNZ27_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY8663010.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56655..57275)
                     /gene="sodA"
                     /locus_tag="JNZ27_000547"
     CDS             complement(56655..57275)
                     /gene="sodA"
                     /locus_tag="JNZ27_000547"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY8663011.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57560..58594
                     /gene="rhaT"
                     /locus_tag="JNZ27_000548"
                     /pseudo
     CDS             57560..58594
                     /gene="rhaT"
                     /locus_tag="JNZ27_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58591..59528)
                     /gene="rhaR"
                     /locus_tag="JNZ27_000549"
                     /pseudo
     CDS             complement(58591..59528)
                     /gene="rhaR"
                     /locus_tag="JNZ27_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(59512..60348)
                     /gene="rhaS"
                     /locus_tag="JNZ27_000550"
     CDS             complement(59512..60348)
                     /gene="rhaS"
                     /locus_tag="JNZ27_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY8663012.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            60636..62105
                     /gene="rhaB"
                     /locus_tag="JNZ27_000551"
     CDS             60636..62105
                     /gene="rhaB"
                     /locus_tag="JNZ27_000551"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY8663013.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62102..63290
                     /locus_tag="JNZ27_000552"
                     /pseudo
     CDS             62102..63290
                     /locus_tag="JNZ27_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            63532..64356
                     /gene="rhaD"
                     /locus_tag="JNZ27_000553"
     CDS             63532..64356
                     /gene="rhaD"
                     /locus_tag="JNZ27_000553"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY8663014.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64366..64680
                     /gene="rhaM"
                     /locus_tag="JNZ27_000554"
     CDS             64366..64680
                     /gene="rhaM"
                     /locus_tag="JNZ27_000554"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY8663015.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            64981..>65048
                     /locus_tag="JNZ27_000555"
     CDS             64981..>65048
                     /locus_tag="JNZ27_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY8663016.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15862 a        17516 c        16642 g        15028 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac accgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa acaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccagcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcataac
    14941 aatgctgccc tgctgcatcc cctgaagcca ctgagtcagg ctgttggtca acgcccctac
    15001 aggctcgccc aagccccaca tcatgatgcc tgcggtaatc agtgtgccga tgataggaat
    15061 aatgaagatg ggcatcaccg agcgcagaac tttatgcacc ggaattttct tcaggtaatg
    15121 caccacgatg ccgccgataa tcccggcgat cagcgcacca aagaagcccg caccaaagct
    15181 gttaccaacc caggcaccga tagcgcacgg agccagcgca gaacgctctg caatggagta
    15241 accaatgtaa gcggcgagga aaggcaccat cagcgtcaaa cctgcaacgc caatatcaaa
    15301 cagttttttc aaattcggat cggctacggc atccggcact gcgcctttgc catacaacat
    15361 gacggaaacc gccagcaaaa taccgcccga taccacgaag ggaatcatgt gtgaaacgcc
    15421 cgtcattaaa tgctgacggg tatttttcag gatctgcacc aactcattca taagctgctc
    15481 ccgggcttgc aaaagcccat gtccatattt ttgatggagg aaacaatagg caatcgcagg
    15541 cggagcaaac agtggataaa aaggccattt actggacttt tgtaatatca gtacaaaaat
    15601 gcgatccgcc tcataacttg cgataatccg gaagcatgcc gcaaaacggc gtcttgtgag
    15661 acgcgtcgca aattgacggt tacattacat ttgtccagct tttggcagat ttgtcacatg
    15721 gcaggccgtt accgtgcgct cttatcctgt aaacagaaaa ccgtaccggg agaaaggcaa
    15781 tggccctgat tgtggaattt atttgtgagc tacctaacgg cgtacatgcg cgtccggcaa
    15841 gccacgttga aacgctgtgt aatacttttt catctcaaat tgagtggcat aacctgcgta
    15901 ctgaccgtaa aggcaacgcc aaaagcgctc tggcgctgat tggcaccgat acgctggtgg
    15961 gtgataactg ccagctactg atttccgggg ccgacgaaca ggaagggcac cagcgtttaa
    16021 gccaatggct gcgcgatgaa ttcccccact gcgatgcgcc gctggcggaa gttaaatctg
    16081 acgaactgga accactgccg gtttcactga ccaatctgaa tccgcaaatt atccgcgccc
    16141 gcaccgtgtg cagcggtagt gcaggcggca ttctgacgcc gatctcttct ttagatctca
    16201 atgcgctgag taatcttccc gcagccaaag acgttgacgc cgagcaatcc gcactggaaa
    16261 acggcctgac gctggtactg aaaaacattg agtttcgtct gctggatagc gacggtgcta
    16321 ccagcgcgat tctggaagcg caccgatccc tggctggcga tacttccctg cacgaacatt
    16381 tactggcagg agtcagcgcc ggattaagct gcgccgaagc gattgtcgcc agtgcgaatc
    16441 acttttgcga agagttcgcc cgttccagca gcagatacct gcaagaacgt gccctggacg
    16501 tacgcgacgt ctgcttccag ttactccagc aaatctacgg tgagcaacgc ttcccggcac
    16561 cgggcaaact gacgcagtcc gccatttgta tggctgatga actgaccccc agccagttcc
    16621 tcgaactgga taaaaatcac ctcaaaggat tgttactgaa aagcggcggc accacctcac
    16681 atacggtgat ccttgcccgt tcgttcaaca ttccgacgct ggttggtgtg gatattgatg
    16741 ccctcactcc gtggcagcat caaacgattt atatcgacgg caacgccggg gcgattgtgg
    16801 ttgagccagg agaagccgta gctcgttatt atcagcaaga agcccgcgta caggacgccc
    16861 tgcgtgagca acagcgtgtc tggctgaccc aacaagcccg taccgctgac ggtatccgca
    16921 ttgaaattgc cgctaacatc gctcactccg tggaagcgca ggccgcattc ggcaatggtg
    16981 cggaaggcgt tggtttgttc cgcactgaaa tgctctatat ggatcgcacc agtgcaccgg
    17041 gcgaaagcga gctgtacaac attttttgtc aggcgctgga gtctgccaac ggacgcagca
    17101 ttattgtgcg cactatggac attggcggcg acaaacccgt tgattatcta aacattcccg
    17161 cagaggcaaa cccgttcctc ggttatcgcg ccgtgcgtat ttatgaagaa tacgcgtcgt
    17221 tatttaccac acaactacgc tcgatcctgc gcgcctccgc tcacggcagc ctgaaaatca
    17281 tgatcccgat gatctcctca atggaagaga tcttatgggt gaaagaaaaa ctggcagaag
    17341 ccaaacagca actacgtaac gaacacattc cgtttgatga gaagatccag ctcggcatta
    17401 tgctggaagt gccgtcggtg atgttcatca tcgatcaatg ctgcgaagag attgatttct
    17461 ttagtattgg tagtaatgac ctgacgcaat atctgctggc agtggatcgc gataacgcta
    17521 aggttactcg tcactacaac agcctgaatc cggcattctt gcgggcgctc gattacgccg
    17581 tgcaggcggt gcatcgccag ggtaaatgga ttggtctgtg cggtgagctg ggagcgaaag
    17641 gttccgtgct gccgttgctg gtcggcttag ggctggatga actcagcatg agcgcaccat
    17701 caattccggc ggcgaaagcg cggatggcgc aacttgatag ccgtgagtgc cgcaagttgc
    17761 tcaaccaggc aatggcctgc cgtacttcgc tggaagtgga acacctgctg gcgcaattcc
    17821 gcatgaccca acaggacgca ccgctggtca ccgccgagtg catcacgctg gaaagtgact
    17881 ggcgcagcaa agaagaagtg ctcaaaggca tgaccgataa cctgctgctg gcgggccgct
    17941 gccgctatcc gcgtaaactg gaagccgact tatgggcgcg cgaggccgtt ttctctaccg
    18001 gtctgggctt tagttttgcc attccacaca gcaaatcaga acacattgag caatccacca
    18061 tcagcgtggc gcgtctgcaa gcgccggtgc gctggggcga tgatgaagcg caattcatca
    18121 ttatgttaac cctgaacaaa cacgctgcgg gcgatcagca catgcgcatt ttctcgcgcc
    18181 tcgctcgccg catcatgcac gaagaattcc gtaacgcgct ggttaacgcc gcctctgccg
    18241 acgctatcgc cagcctgctg caacatgaac tggaactgta aaaggaaaca tcatggaact
    18301 gtatctggac accgctaacg tcgcagaagt cgaacgtctg gcacgcatat tcccgattgc
    18361 cggggtgaca actaacccga gcattatcgc tgccagcaag gagtccatct gggaagtgct
    18421 gccgcgtctg caaaaagcga ttggtgatga gggcattctg tttgctcaga ccatgagccg
    18481 cgacgcgcag gggatggtgg aagaagcgaa gcgcctgcgc gacgctattc cgggtattgt
    18541 ggtgaaaatc ccggtgactt ccgaaggtct ggcagcaatt aaaatactga aaaaagaggg
    18601 tattactaca cttggcaccg ctgtatatag cgccgcagaa gggttattag ccgcactggc
    18661 aggggcaaaa tacgttgctc cgtatgttaa ccgcgtagat gcccagggcg gggacggcat
    18721 tcgtacggtt caggagctgc aaacgctgtt agaaatgcac gcgccagaaa gcatggtgct
    18781 ggcagccagc tttaaaacgc cgtgtcaggc gctggactgt ttactggcag gatgtgaatc
    18841 catcaccctg cccttagatg tagcgcaaca aatgctcaac acccctgcag tagagtcagc
    18901 tatagagaag ttcgaacacg actggaatgc cgcatttggc actactcatc tctaaaggag
    18961 caattatgga ccgcattatt caatcaccgg gtaaatacat ccagggcgct gatgtgatta
    19021 atcgtctggg cgaatacctg aagccgctgg cagaacgctg gttagtggtg ggtgacaaat
    19081 ttgttttagg ttttgctcaa tccactgtcg agaaaagctt taaagatgct ggactggtag
    19141 tagaaattac gccgtttggc ggtgaatgtt cgcaaaatga gatcgaccgt ctgcgtggca
    19201 tcgcggagac tgcgcagtgt ggcgcaattc tcggtatcgg tggcggaaaa accctcgata
    19261 ctgccaaagc actggcacat ttcatgggtg ttccggtagc gatcgcaccg actatcgcct
    19321 ctaccgatgc accgtgcagc gcattgtctg ttatctacac cgatgagggt gagtttgacc
    19381 gctatctgct gttgccaaat aacccgaata tggtcattgt cgacaccaaa atcgtcgcgg
    19441 gcgcacctgc acgtctgtta gcagcgggta tcggcgatgc gctggcaacc tggtttgaag
    19501 cgcgtgcttg ctctcgtagc ggcgcgacca ccatggcggg cggcaagtgc acccaggcgg
    19561 cgctggcact ggctgaactg tgctacaaca ccctgctgga agaaggcgaa aaagcgatgc
    19621 ttgccgccga acagcatgta gtaaccccgg cgctggagcg cgtgattgaa gcgaacacct
    19681 atttgagcgg cgttggtttt gaaagcggcg gctcacgcgg tgcataacgg cctgaccgct
    19741 atcccggacg cgcatcacta ttatcacggt gaaaaagtgg catttggtac gctgacgcag
    19801 ctggtactgg aaaacgcgcc ggtggaggaa atcgaaaccg tagctgcact tagccatgcg
    19861 gtaggtttgc caataactct cgctcaactg gatattaaag aagatgtccc ggcgaaaatg
    19921 cgcattgttg caaaagcatc ttgtgcagaa ggtgaaacca tccacaacat gcctggcggc
    19981 gcgacgccag atcaggttta cgccgcactg ctggtagctg accagtacgg tcagcgtttc
    20041 ctgcaagagt gggaataacc tactccaaac tcccggcttg tccgggagtt tgaacgcaaa
    20101 attgcctgat gcgctacgct tatcaggcct acgcaatctc tgcaatatat tgaatttgcg
    20161 tgcttttgta ggccggataa ggcgttcact ccgcatccgg cactctgtgc cagtaatctg
    20221 aaactcccac gttgttcagg agttttttat ccgcttctgg acattttctc cacagaacaa
    20281 tcgttagccc ggaaggtcga atctgtgact cttattttca cggacgaacc agatgaaagc
    20341 ttcactggcg ctgctcagcc tgctaacggc atttaccagc cactccttaa aatctcccgc
    20401 cgttccacca accgtggtac aaatccaggc caataccaac cttgccatcg cagacggtgc
    20461 cagacagcaa attggcagca cgctatttta cgatccggcg tatatgcagc ttacctatcc
    20521 tggcggtgat gttccgcaag aacgcggtgt atgttccgat gtggtgatcc gcgcattgcg
    20581 cagccagaag gtcgatttgc agaaactggt tcacgaagat atggcgaaga attttgctga
    20641 gtacccgcaa aaatggaagt taaagcgccc ggacagcaac atcgatcacc gtcgggtgcc
    20701 taatctggaa acctggttta gccgccacaa caaaacacgc cccatcagta aaaatgccgg
    20761 tgactatcaa gcgggcgata ttgtctcctg gcgattagat aacgggctgg cgcatattgg
    20821 ggtggcgtca gatggcttcg cccgcgacgg tacgccgctg gtgatacata acattggcgc
    20881 aggcgcgcag gaagaagatg tgctgtttag ctggcggatg gtggggcatt atcgttattt
    20941 tgttaaataa gagatcgccc cacatgaaga aaaaattcag atgcttttta cactgacagc
    21001 ttcttttttc ttacggctca ccagcgccag tgccagcaca atcagcacaa taccgctacc
    21061 ttcaacggcc cccggattct caccgagcag ccaccaggaa aagagtacgc cacaaacggg
    21121 aacggccagt gtgcttaagc tggcaatact ggcaggcaag tttttcagca caaacagcca
    21181 taagctccac gctagcgccg tcgccagaat cgcactgtag gccagcgccc agaacacggt
    21241 gggctgccag tcaatttcac gttgcggcac cagtaaagcg accacactca tcaccagcgc
    21301 cgcatacagc atctgccagg atgttaacga taataaatcc acgcgcggat gacgggcgta
    21361 taggcgttta gcaacaatcg cgctcgcccc ccaactaaca ccggagagga ttgccagcat
    21421 ggcacttttc atcgaagaga aatcgagttg ccacggctgc aacaccaaaa ataagccgaa
    21481 agcggcaatc agaatcgcga aatattgccc acgtcgcagg cgttcaccga gaaacaacgc
    21541 ggcgaaaatc accacccaga acggcatggt atagctcagg atcgccacct tccccgcacc
    21601 tccgctgacc aacgcccact gcgccagacc aaccatcccg caggtttgta acagggcaat
    21661 ggctaaggtg tatttaaacg gcgtcgggcg cattccgcga ccacgtaata aaaggacgat
    21721 gaataaaacg agagcgccga aaatgcagcg taaggcggta aagtcgaagg caccgatgta
    21781 actggtgact tgcttcatga aaatccagct ataactccag ataagtgtga gcacaaccag
    21841 gccactgatt gccagtgggt tgctctttcc tgcggcagac atgagagatc cagtgtgtag
    21901 ttttcctgtg gacactatac gccggtaaag aaacttcagc gactgcctga aagacagtcg
    21961 ctgaagaagg tcagattaca gcaggtcgaa acggtcgagg ttcatcactt tcacccatgc
    22021 cgctacgaag tctttaacaa acttctcgtg ggcatcgctg ctggcgtaaa cttccgctac
    22081 tgcacgcagg acagagttag aaccaaatac cagatccgca cggctggctg tgtatttcac
    22141 ttcgccggtt tcgcggtcac gtccttcgaa cagctctttc gattcgtcag tcgctttcca
    22201 ctcgtaacgc atatccagca agttcacgaa gaagtcattg ctcaatacgc caacgcggtc
    22261 agtgaagacg ccgtttttgc tgccatcgaa gttggcaccc agtacacgca tcccgcccac
    22321 cagcgcagtc atttccggcg cggtcagcgt cagttgctgt gctttgtcga tcagcagtga
    22381 ctcggtggtg gaaacgtcca gacgagcgcg atagttacgg aaaccgtcag caattggctc
    22441 cagcagctca aacatctcaa tgtcagtctg atcctgacgc gcatcaacgc gacccggcgc
    22501 aaacggtaca tgaatgctca aacctgcggc gcttgcggct ttctcaacac caaccacacc
    22561 agccagcacg atgatatccg ccagcgaggc tttaccagac tctttctgga ttttctccag
    22621 aacaggcaga gcacgaacgg ctgcggcgtt cacatcccag tcgcgctgcg gcattaatgc
    22681 cagacgcgca ccgttgacac caccgcgttt gtcgccacca cggaaggtag aagcagatgc
    22741 ccaggccacc gataccagct cactaacaga cagaccagaa tccgcaatcg cgaatttcag
    22801 atcgataatg tcctgctcgg tcgggttgta gatcggctgc ggcagcggat cttgccagat
    22861 cagatcttct ttcggcactt ccggcccgat gtagcgagat ttcggcccca tatccctgtg
    22921 cgtcagtttg aaccaggcac gggcaaaggc ttcgttgaac gcctgcggat cgttgaggaa
    22981 acgacgagag atcttctcga actcaggatc aaaacgcagc gtcaggtcgg tcaccagcat
    23041 tgtcggttta cgtttcttcg acggatcgaa cggatccggg ataatttccg gtgcgtctac
    23101 cgcttcgaac tggattgctc cagccggact gcgggtctgt acccactcat acttgaacag
    23161 attctcgaag aaatagttgc tccactgggt cggcgtctgg gtccagacta cttccagacc
    23221 agaggtaatg gcatctgcgc caacgccgct gccgtaagtg ctcgcccagc ctaaaccttg
    23281 ttcttcaatc ggtgcagctt ctggatcagg acctacgttt gatgtcggac cggcaccgtg
    23341 ggttttaccc agcgtatgac cacccgcaat cagcgccacg gtttcttcgt cgttcatgcc
    23401 catgttgccg aaggtcgcgc ggatagctgc tgccgcagaa agcggttcgc cgctgtgatc
    23461 cgggccttcc gggttaacgt aaatcaggcc catctcggtt gcacccagcg gtgctttcgc
    23521 cagcgcttcc ggatgacggt gagtcagcca ggctttttca tcaccccagt taacatccag
    23581 atccggttcc cagacgtctt cacgaccggc accaaaaccg aaggtacgga agccggagtt
    23641 ttccagcgcc acgttacctg cgaggataaa caggtcggcc caggagattt tctgaccata
    23701 tttctgtttg attggccaca acaggcgacg cgctttatcg aggcttacgt tatccggcca
    23761 ggagttcagc ggtgcaaaac gttgctgacc acgacccgcg ccgccgcgtc catcgattga
    23821 acggtaagta cctgcgccgt gccaagccat acgaataaac agaccggcgt agctgcccca
    23881 gtcggctggc caccacggct gagattctgt cagcagggct ttcagatctt ttttcaggcc
    23941 gtagtaatct aatttgctga attctttgcg gtagtcaaag tcctcaccca gcgggttaga
    24001 acgattagaa tgttggttta acaggtcaac acggagttga tttggccacc agtcgcgagt
    24061 ggttgtgccc gcccccgcgc tctggtcgtg accgccctga tggaacgggc atttgccagt
    24121 ggctgtggtg ttatggatat cgtctgacgt gctcatcaat gtgctcccct ctacagtgtt
    24181 accgttacga tacacagcgt tagagagaag ttataattga attaatccac ggatgcgata
    24241 gttgagatct tacatattgt tggttaaaga gatgtagatc aaattgatcg taattaagct
    24301 tttttgatta tgtccgaatt cggacaaatg ctttataaaa aagggtttta gcttcatatc
    24361 cttcaagtaa tgagggagaa aacaaggctg acaaccttat tttcccgccc tcatttcgag
    24421 gcagcatttt gtgctctgtt taaaatttgt gatcactgtg tgattttcac aaaagccaca
    24481 ctatttataa accaggtcga acacccagcg tatggcaaat cgcgtaactc atttcagcgc
    24541 ggttaagcgt atagaagtgg aaatctttca ctccttcacg gcttaaaatc ttcaccatat
    24601 ccatggcaat attcgcgccc accagtttgc gggtttcggc atcgtcatcc agcccgtcaa
    24661 acatttgcgc catccacgcc ggaatacgca cgttggtcat gtcggcaaat ttcttcgcct
    24721 gtttaaagtt agataccggc aaaatgcccg ggatgatttc cacatcaatg cccgccgata
    24781 cacagcggtc acgaaaacgc aggtagcttt cgacatcgaa gaagaactga gtaatcgcgc
    24841 ggttggctcc ggcatccact ttgcgtttca gattaagcaa atccgcctga gcgctttttg
    24901 cttccgggtg aacttccgga tacgccgcca cggatatatc gaaatctgcc acttctttta
    24961 acagcgtcac caggtcagaa gcatacattt ctggcttacc acttcccggc ggcagatcgc
    25021 cacgcagtgc cacgatatga caaataccgt tattccagta gtcgcgtgca atggtgcgca
    25081 gctcgtcggg cgtcgcatca atgcaagtaa gatgcggtgc cgcttccaga ccagtgcgat
    25141 ctttaatgcc tttaataatg ctgtgcgtac ggtcgcgctc gccggagttc gcgccatagg
    25201 tcaccgatac aaacttcggt ttcaggctgc taaggcgatc gatggagttc cacagggtct
    25261 gctccatttc actggtacgc ggcgggaaaa actcgaacga aacgttaatc tgcccctgga
    25321 cttctgccag gctctgattc agggcatccc gctggctggc gtgaaaaaag ctcatacctt
    25381 acctcatcaa tcgcttgtag ttatatgttg tgttgtgcac atctatccat ttagacgtcc
    25441 agatgtaaag atgaaggaaa agccgaaggg cgtcaaccga aaaatgacca atgatggtga
    25501 ggaaaaatca gggaagatga aaaaacttca ggatggtaag aaaaagaaaa tccgatatgc
    25561 aaagcaaacc ggactgcaaa tataaactaa ccatgtactt attgcttctt gccggattcg
    25621 tcgtaaacgc ttcatccggc ctaattctat gataattcaa tatatttcat tagaaaccat
    25681 agacccgaaa acatgagtac cgggcattat taaatttctg aaattacaac aactgtgcca
    25741 gccggttgat atccgactga atcgccccgg cggtgacgtc gcgcccagcg ccaggcccac
    25801 ggatcaccag cggattatcg cggtaccagc ggctttcgat ggcaaagacg ttatcgcatg
    25861 gcagcagtga tgccagcgga tggtcttcac gcaccgcctc cacgcccaca cgcgctttgc
    25921 cgttggcatc gaaacgcgcc acgtagcgca gcaccagccc catttcgcgg gccgcttcca
    25981 gccgttgcac catctgctcg ttaagttcat cgccattttc aaagaaatgg tcgatgctgc
    26041 caccttcgca atgagcaggc accagcgatt ccacacgtac ctggtccggt tcaatgttgt
    26101 aacccgcttc acgcgccaga atcaccagct tgcgcattac gtctttgccg gagaggtcgt
    26161 cacgcggatc aggctcggtt aagccctgct gccacgcctg atccaccagc tcggtaaacg
    26221 gcacgctacc gtcgaattgc aggaacagcc aggagagcgt gccggagaag atcccactga
    26281 tcgacaaaat ggtatcgccg ctgtcgatca gatcgcgcac ggtatggttg atcggcaagc
    26341 ccgcaccaac ggtggcgttg tatagccagt gacgcccggt tttttcgaag gcgtcgtgga
    26401 tctggcgata tttattgctg tcgcttgctc ccgccagttt gttggcactg atgacgtgga
    26461 aaccgtggct ggcgaaatcg agatactgat cagcaagctg ctggctggcg gtaacgtcca
    26521 acaccactaa atcatcatac ggatgggcgc gcatccacag gaaaagcgac tcttcatcct
    26581 gctcgaccgc ttcatcgttg aagaaggcta acgcgcggct ggcgtccagc ccgtcataac
    26641 tcaacagact gcggcggctg tccaccacgc ctgccagcac aaattcaaag ccagtacgcg
    26701 ccgaaagcgt gctctgctcg cgggcgaaca gttccagcca gcgagagccg atattgcctt
    26761 tgccgaacaa taccaggccg atgcgttttt ctgcgcggaa gacggactga tgcagcccct
    26821 gaatcaggct ttcggtcggg ccggtgcgca gtactgccac cagactgatg ccgtcatcgg
    26881 actgccacgt aaactcgacc ggctggcctt tcagttgctg ccagaagcgg tggcaatgca
    26941 gcgggttacg ggtgacaccc gccccgacca tcgccaccag cgccagcccc tgacgcaggc
    27001 gcagttctcc aggtaatccc gcttcgtcga ggattttcag cgcactgtcg gccacttctg
    27061 aggtgtagca aaattgcagc aactggcgat cgttatgcac gccaaccgcc agtggacgca
    27121 cctgggcacg tttcaggatc tggtcgatct ctttatgcgc cagtttgaaa tcctgactgg
    27181 cgggcacctg aaactcaatc aaacagacat catcgtggct ggtgacaata cgcgcaccag
    27241 taccggaggc cagcacgcgt tcaatgcgcg tggaaccttg atccggcgtg tagctacagc
    27301 gcagctgcag gtcgatttcg ctgccagaaa ctggctgtaa agtacgggcg tgaagaacgg
    27361 gagccgccag acgcgccagt tcgctggcct catccagacg cagcaacggc agcaggcagg
    27421 catctttcac tttacgcggg tcggcactgt ataccccggc gacgtcgctc cagatggtta
    27481 cacgagaaac acccgccagc gcaccgattt gtgtcgcgga atagtcggaa ccgttacgcc
    27541 ccagcaacac cgtttcaccg gcgttgttac ggctgataaa cccggtcacc accagacgtt
    27601 tgctcggatg ttgtaccagt agctgttgca gcaacgggta agaaagccct tcatcaacct
    27661 gcggttgcgc agcgcgttca gcgcgtaaaa actcacgggc atcaagccag gccgctggca
    27721 gcccttgttg attaagtacc gcagacatca ggcgcgccga ccacacttca ccgtggccca
    27781 ccacttccgc atacaccgca tcgttaatac cactgtcgag caacgccgcc aggcgctcaa
    27841 gatcgctgac aaaagcgcta atgaggctat cggcttcttc agcgggtagc agaccgctaa
    27901 tcagatcgca ctgatagcga cgcagcgttt gttgaacctg atgcgcagag agacgatcgg
    27961 tctggcttag tttcaaccag ttaatcaact ggttagtggt gctaccggcg gcggaaacca
    28021 ccatcatatc gtcaggctga gagaactccg ccataatgcc cgcgacacgc aaataacact
    28081 tcacatcagc cagactactg ccaccaaatt tatgcagctg acgacctttc gcccctgcct
    28141 gcgcaatcac actcattttt accccttgtt tgcagcccgg aagccatttt ccaggtcggc
    28201 aattaaatct tcgccatctt caataccggt ggagatacgc agcagcgtct cggagatccc
    28261 ggcggcagca cgcgcttctg gtgccatgcc tgcatgtgtc atggttgcgg cgtgagagat
    28321 taaactttcc actcccccta atgattccgc cagcgtaaac aacgacagcc cgcccaggaa
    28381 acgacacagc gtctgctcat cgccatccag ttcaaaactc aacattgcgc caaagccttt
    28441 ttgctgacgc gcggcaattt catgcccctg attttccggc agcgacgggt gatacagttt
    28501 tttcaccaac ggctgggttt gcagatattt cacaatcgcc tgcgcgttgc gctgcgccag
    28561 ctccatacgc ggcaccagcg ttcgcaaccc gcgtagcagc agatagctgt caaacgcgcc
    28621 gcccgtcacg ccaatattgt ttgcccacca ggcaagttca gtgacaacgt ccgggtcttt
    28681 agcaatcacc acgccagcca ccacgtcaga gtgaccgttc agatatttcg tacatgaatg
    28741 caacaccaga tcggcaccca atgccagcgg attttgtaat gccgggctta agaaggtgtt
    28801 atccaccacg ctcaccgccc cgacttccct tgccagatgg cagattttcg caatatccac
    28861 gacgcgtaac aatggattac ttgggctttc taccagtacc agtttgggtt tttctgccag
    28921 cgctgcccgt aatgcctgtt catcgccttg atcaacaaac aacacgcgat agcaaccgcg
    28981 tttcgccaga ctgtcgaaca ggcgatagct accgccgtag cagtcgtgcg gcgcaaccag
    29041 cagatcgcca ggtttcaaaa agacggtcgt taccaggtga atcgcggaca tgccggtatt
    29101 agtaagtact gcgccagcac cgccttccag ttctgccagc gcacgctgaa ccacatcgcg
    29161 cgttgggttg ccgcgacgcg agtaatcatg cgcgcgcggt tcattaaatc cggtaaagtt
    29221 ataggtgctg gaaagatgga tgggagggac aacgcaacca tactgttcgt cgtcatttaa
    29281 cccgctacgc actgcgatgg tggcctgttt acgcgtcatg tgatgaagtt tcctgggctt
    29341 tgtcggtgaa atgtcaggca ccagagtaaa cattgtgtta atggacgtca atacatctgg
    29401 acatctaaac ttctttgcgt atagattgag caaatcccaa atagccgtta aaattatatt
    29461 cattatcacg ccgacaggtg cattacacga tgtcacggta acgcctgtac ggtaaactat
    29521 gcgggtttac ggtcagtacc cacataaatt gtggggtctg gtctcaattt attgacgaag
    29581 aggattaagt atctcatggc tgaatggagc ggcgaatata tcagcccata cgctgagcac
    29641 ggcaagaaga gtgaacaagt caaaaagatt acggtttcca ttcctcttaa ggtgttaaaa
    29701 atcctcaccg atgaacgcac gcgtcgtcag gtgaacaacc tgcgtcacgc taccaacagc
    29761 gagctgctgt gcgaagcgtt tctgcatgcc tttaccgggc aacctttgcc ggatgatgcc
    29821 gatctgcgta aagagcgcag cgacgaaatc ccggaagcgg caaaagagat catgcgtgag
    29881 atggggatta acccggagac gtgggaatac taaagcggaa aagagcggcg cggagtggaa
    29941 tcgcctgatg cactacgctt atcaggccta cgtcatattg caatttattg aatttgcacg
    30001 aacttgtagg ccggataagg cgttcacgcc gcatccggca taaacaacga gcacgttgtc
    30061 tgcgacccac cgctttttat acatggacgt ttaactatga aaaacaggct gctgatcctc
    30121 agcctgctgg tttctgtacc tgcctttgcc tggcagccgc aaactggcga catcatcttt
    30181 caaatctctc gctcatcgca aagtaaagcg atccaactgg caacccattc cgattacagc
    30241 cacaccggta tgctggtgat gcgtaacaaa aagccctacg tttttgaagc ggtcggcccg
    30301 gtgaaataca ccccgctcaa gcagtggatt gcccatggtg aaaagggcaa ctacgttgtt
    30361 cgccgcgttg aaggcggatt gagtgttgaa caacagcaaa aactgacgca aacggcaaaa
    30421 cgttatctcg gtaaaccgta tgatttcagc ttctcatgga gcgacgatcg ccagtactgt
    30481 tcggaagtgg tgtggaaggt ttaccagaac gcgctgggaa tgcgcgtggg cgaacagcag
    30541 aagttgaaag agtttgatct cagcaacccg ctggtccagg cgaagctcaa agaacgctac
    30601 ggtaaaaata ttccgcttga ggaaacggta gtttcaccac aagccgtttt cgacgcgcca
    30661 caactcacca cggttgccaa agagtggccg ctgttttcgt ggtgatacga ccaaaattca
    30721 ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc cggtcttatt tgctgcccgg
    30781 gatgttgaaa cgcttgttga agcggtcaac acggccaccg gtagcaacat cacgctgttt
    30841 gccagtgaag aacgggtggc acttgctgca cacgtcgagg ttcaggtcat gaccaacggt
    30901 ggagcggatt ttcattacgt taccgcaaga gcagctagca gtaatttctt cgtatttcgg
    30961 gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc gcgtcgctct tccagcccta
    31021 acgccagaca ccacgcgatg ttaaaagtat agcttcaata cgatcatttc gtacgaaggc
    31081 gcgaaatcat acagaaatta accagcgtat gcaaactgat ccgcactctt ctacggcaat
    31141 gtgtatacta acccaccgaa tttcaagtca ggatgatgct atgcccgttg cccacgttgc
    31201 cttgcccgtt ccgcttcctc gtacctttga ctatctgctg ccagaaggca tgacggttaa
    31261 agctgggtgt cgcgtgcgcg tgccgtttgg caaacagcag gagcgcatcg ggattgtggt
    31321 atcagttagc gatgccagcg aactgccgct cactgagcta aaagcggtag tcgaagtgct
    31381 ggatggtgag ccggtgttta ctcactccgt ctggcgattg ctgctatggg cggcagatta
    31441 ctatcatcat ccgattggcg atgtgctgtt tcatgccttg ccgattttac tacgccaggg
    31501 gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc actgaacaag gtcaggcggt
    31561 ggatctgaac agcctgaaac gctcacccaa gcaacaacag gcgctggcag cgttacggca
    31621 aggcaaaatc tggcgcgacc aggttgccac gctcgaattt aatgatgccg cgttgcaggc
    31681 gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca ccagagttta gcgactggcg
    31741 aacgaactat gccgtttctg gtgagcggtt gcgattgaat accgaacagg ccaccgccgt
    31801 tggcgcaatt catagcgcgg cagatacttt ttctgcctgg ctgctggcgg gcgttaccgg
    31861 ttccggtaaa acggaggttt atctcagtgt actggaaaac gtgctcgctc agggcaaaca
    31921 ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa actatcgccc gttttcgtga
    31981 acggtttaat gcgcccgtgg aagttctgca ttccggtctg aacgacagcg agcgtctttc
    32041 ggcgtggctg aaagcgaaaa atggcgaggc ggcgattgtg atcggcaccc gctccgcgct
    32101 gtttacgccg tttaaaaatc tcggcgtgat tgtcattgac gaagagcacg acagttccta
    32161 caagcagcag gaaggctggc gctatcatgc ccgcgacctg gcggtgtatc gtgcgcatag
    32221 cgagcaaatc ccgattattc ttggctccgc aacgcccgcg ctggaaacgt tgtgcaacgt
    32281 ccagcagaaa aaataccgcc tgctgcgcct gacgcgccgg gcggggaatg cgcgtccggc
    32341 aattcaacac gtgctggatt taaaaggtca gaaggtgcag gcaggtctgg ctccggcgtt
    32401 aatcacccgt atgcgccagc atttacaggc caacaatcag gttatcctct ttcttaaccg
    32461 ccgtggcttt gcgcctgcgc tactgtgcca cgactgcggc tggattgccg aatgcccacg
    32521 ttgcgatcac tactacacgc tgcatcaggc gcaacagcat ttgcgctgtc accactgtga
    32581 cagtcagcgt ccggtgccgc gccagtgccc ttcctgcggt tccacgcacc tggtacccgt
    32641 ggggctgggc accgaacagc ttgaacagac gcttgcgccg ctgttccctg atgtgcccat
    32701 ttctcgtatc gaccgcgata ccaccagccg caaaggggcg ctggaacagc aactggcgga
    32761 agtacatcgc ggcggtgcga ggattttgat cggcacacaa atgctggcga aaggtcacca
    32821 ctttccggat gtgacgctgg tggcgttact ggatgtggac ggcgcgctgt tttctgccga
    32881 ttttcgctcg gcagagcgtt tcgctcagct ttacacccag gtcgctggtc gtgccgggcg
    32941 tgcgggtaaa caaggcgaag tggtgctgca aacgcaccat ccggaacatc ctctgttgca
    33001 aacgttgctc tataaaggct acgacgcctt tgccgaacag gcgctggctg agcgacggat
    33061 gatgcaactg ccgccatgga ccagccatgt gattgtgcgt gcggaagatc ataacaatca
    33121 gcatgcgccg ctgttcctgc aacaactgcg taatctgatc ctctccagtc cgctggcaga
    33181 cgacaaactg tgggttctcg gtccggttcc ggcactggca cctaaacgtg gcggtcgctg
    33241 gcgctggcag atattgttgc agcacccttc ccgcgtgcgc ttgcaacaca tcattagtgg
    33301 tacgctggcg ctcatcaata caataccgga ttcccgcaag gtgaaatggg tactggatgt
    33361 tgatccgatt gagggttaaa ccgcttacga tgcgaggcgg atcgaaaaat tcaatattca
    33421 tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc tatctgctaa aaatgttgcc
    33481 gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca ggcgaggagt gagtgtgaaa
    33541 gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg ccctcaaggc aaaagtctct
    33601 acagcgactg tctcccgagc attaatgaat cccgataaag tctcccaggc cacccgtaat
    33661 cgggttgaaa aagcggcccg ggaagtgggt tatttaccgc agcctatggg gcgcaacgtc
    33721 aagcgtaatg aatcccgcac cattctggtg attgtcccgg atatctgcga tcccttcttt
    33781 agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc acggatatct ggtgctgatt
    33841 ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta tcgatttgat catcaccaag
    33901 caaattgatg gcatgttgct gctgggttca aggctgccgt ttgatgccag cattgaggaa
    33961 cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg caccggaact ggagctgcct
    34021 accgttcata tcgacaatct gaccgccgca tttgatgcag taaattattt atatgagcaa
    34081 gggcataaac ggattggctg tatagccggt cccgaagaga tgccactgtg tcactaccgc
    34141 ctgcaaggct atgttcaggc gctgcgtcgc tgcggaatta tggttgatcc acaatacatc
    34201 gcccgtggcg acttcacctt cgaagccgga agcaaagcaa tgcaacagct gcttgatctt
    34261 ccacaaccgc ctactgcagt cttctgccat agcgatgtga tggcgctcgg cgcactttct
    34321 caggcaaaac gccaggggct gaaagtcccg gaagaccttt ccataatcgg ttttgataac
    34381 atcgacctga cgcaattttg tgatccgccg ctgacaacca tcgcgcagcc gcgttacgaa
    34441 atcggtcggg aagctatgct gttattgctt gatcaaatgc aggggcaaca cgttggcagt
    34501 ggctctcgtt taatggactg cgaacttatc atccggggat caacacgcgc gttaccttaa
    34561 agtaaacctt taagactgcc gtgactggtc aaaggcccgc cgcttaagta acatggcggg
    34621 ctgacgaacg aataaataca gcgaaacgat agtggcacaa cgagattatg tacgccgcag
    34681 ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg aaaaagcaac gaaatctgcc
    34741 tgcggtttct cccgctatgg tcgctattgc tgccgccgtt cttgtgacct ttatcggtgg
    34801 tctgtacttc attacgcatc acaagaaaga agagtccgag acgctgcaaa gccagaaagt
    34861 gaccggaaac ggactaccac caaaaccaga agaacgctgg cgctacatta aagagctgga
    34921 aagtcgccag ccgggtgtgc gtgcgcccac agaaccttct gccggtggtg aagtgaaaac
    34981 gccggagcaa ctgacaccag aacaacgtca gcttcttgaa caaatgcagg ctgatatgcg
    35041 ccagcagcca acgcagctgg ttgaggttcc gtggaatgaa cagacgccgg aacagcgtca
    35101 gcaaacgcta caacgccagc gtcaggcgca gcaattagcg gagcagcaac gcctggctca
    35161 gcagtcgcgt acgactgaac aaagctggca gcagcagacg cgtacgtcgc aagccgcgcc
    35221 ggtacaggcg caaccgcgcc agtctaaacc agcttctact cagcagccgt accaggatct
    35281 gctgcaaacc cctgcgcaca cgaccgcgca gtcgaagccg cagcaagctg cgccggttgc
    35341 tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa gacgaacgcc gctggatggt
    35401 gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg gtacgtgctc agctggcgtt
    35461 cgaaggattt gactcgaaaa tcaccaccaa caatggctgg aatcgtgtgg tcattggccc
    35521 ggtgaaaggc aaagagaacg cagacagcac cctcaatcgg ttgaagatgg cgggtcatac
    35581 aaactgcatt cggctcgccg ccgggggttg aaaccctcaa aatccccccc atctataatt
    35641 gcattatgcc ccgtactttt gtacggggtt tgtactctgt attcgtaacc aaggggtcag
    35701 ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg tggtcatcgc tggtgatggt
    35761 caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga aaaaggtccg ccgtctgtac
    35821 aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg atgcttttac gctgttcgaa
    35881 ctgtttgaac gtaaactgga aatgcatcag ggccatctgg ttaaagccgc cgttgagctg
    35941 gcaaaagact ggcgtaccga tcgcatgctg cgcaaacttg aagcactgct ggcagtcgcg
    36001 gatgaaactg catcgcttat catcaccggt aacggtgacg tggtgcagcc agaaaacgat
    36061 cttattgcta tcggctccgg cggcccttac gcccaggctg cggcgcgcgc gctgttagaa
    36121 aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt tggatattgc aggcgacatt
    36181 tgcatctata ccaaccattt ccacaccatc gaagaattaa gctacaaagc gtaaggatct
    36241 cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac tggataagca catcatcggc
    36301 caggacaacg ccaagcgttc tgtggcgatt gctctgcgta accgctggcg tcgcatgcag
    36361 ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata tcctgatgat cggcccgacc
    36421 ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc tggcgaatgc gccgttcatc
    36481 aaagttgaag cgaccaaatt taccgaagtg ggctacgtcg gtaaggaagt tgattctatt
    36541 attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg tccaggctat cgagaaaaac
    36601 cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg acgtgctgat cccacctgct
    36661 aaaaacaact ggggacagac tgaacagcag caggaacctt ccgctgctcg tcaggcattc
    36721 cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa tcgagatcga tcttgccgca
    36781 gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg aagagatgac cagccagctg
    36841 cagtccatgt tccagaacct gggcggccag aagcaaaaag cgcgtaagct gaaaatcaaa
    36901 gacgccatga agctgctgat tgaagaagaa gcggcgaaac tggtgaaccc ggaagagctg
    36961 aagcaagacg ctatcgacgc tgttgagcag cacgggatcg tgtttatcga cgaaatcgac
    37021 aaaatctgta agcgcggcga gtcttccggt ccggatgttt ctcgtgaagg cgttcagcgt
    37081 gacctgctgc cgctggtaga aggttgcacc gtttccacca aacacgggat ggtcaaaact
    37141 gaccacattc tgtttatcgc ttctggcgcg ttccagattg cgaaaccgtc tgacctgatc
    37201 ccggaactgc aaggtcggct gccaatccgc gttgaactgc aggcactgac caccagcgac
    37261 ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg tgcagtacaa agcactgatg
    37321 gcgactgaag gcgtaaatat cgagtttacc gactccggta ttaaacgcat cgcggaagcg
    37381 gcatggcagg tgaacgaatc taccgaaaac atcggtgctc gtcgtttaca cactgttctg
    37441 gagcgtttaa tggaagagat ttcctacgac gccagcgatt taagcggtca aaatatcact
    37501 attgacgcag attatgtgag caaacatctg gatgcgttgg tggcagatga agatctgagc
    37561 cgttttatcc tataatcgcg ttcaatcatt ttcatcattg tttgatgggg ctgaaaggcc
    37621 ccatttttat tggcgcgtat tatgactgaa caacaaatta gccgaactca ggcgtggctg
    37681 gaaagtttac gacctaaaac cctccccctc gcctttgctg caattatcgt cgggacggcg
    37741 ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc tgctggcact aattaccgcc
    37801 gggctattac agatcctttc taacctcgcc aatgattacg gcgatgcggt aaaaggcagc
    37861 gataaacctg accgcattgg gccgctacgc ggcatgcaaa aaggggtcat tacccagcaa
    37921 aagatgaaac gggcgctcat tattaccgtc gtgctcatct gtctctccgg gctggcactg
    37981 gttgcagtgg catgccatac gctggccgat tttgtcggtt tcctgattct tggcgggttg
    38041 tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc cttatggtta tatcggtctg
    38101 ggtgatattt ccgtactggt tttctttggc tggttgagtg tcatggggag ctggtattta
    38161 caggctcata cattgattcc ggcactgatc cttccggcga ccgcatgcgg cctgctggca
    38221 acggcagtac tgaatattaa taacctgcgt gatatcaata gcgaccgcga aaatggcaaa
    38281 aacacgctgg tggtgcgctt aggtgaagtg aacgcgcgtc gttatcatgc ctgcctgctg
    38341 atgggctcgc tggtgtgtct ggcgctgttt aatctctttt cgctgcatag cctgtggggc
    38401 tggctgttcc tgctggcggc accattgctg gtgaagcaag cccgttatgt gatgcgggaa
    38461 atggacccgg tggcgatgcg accaatgctg gaacgtactg tcaagggagc gttactgact
    38521 aacctgctgt ttgttttagg gatattccta agccagtggg cagcataact gacaaatatc
    38581 aattaacaat tgatgatttt gccaacagcc cacatagcgc gatatactga aaattctcgc
    38641 agcaactgaa tgttaagcct atgaaatacg atacttccga gctttgtgac atctatcaag
    38701 aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg cggacgggcg tcgtttggcg
    38761 gacaaataat cacggtaaaa tgtttcgagg acaacgggtt gctgtacgat ctgctcgaac
    38821 agaatggccg tggtcgtgtt cttgtcgtcg atggcggtgg ttctgttcgt cgcgcactgg
    38881 tcgatgctga actggcgcgt ctggcagtac aaaatgaatg ggaaggtctg gtcatttacg
    38941 gcgcggtgcg tcaggtagat gacctggaag agttggatat cggcatccag gcgatggcgg
    39001 caattccggt tggtgccgct ggcgaaggca ttggcgaaag cgatgtccgc gtcaattttg
    39061 gcggtgtcac cttcttctcc ggcgaccatc tttatgccga caataccggg attattcttt
    39121 cagaagatcc gctggatatt gaatgataag aaaggcaccg caaggtgcct tttttctgcg
    39181 ttacctgttg gcctacacag taaagaaatt acgcgaaaga tgaagcgtaa tcagacctct
    39241 tccatgcgac ccagcagggc ctgcagacgt tcctgccagc cgttctgctg ttctttcaga
    39301 tggttgttct cacgctccag ctcttcgcgc tgatgctggg cattttgaac ttcctgcgac
    39361 agtgagttgt ttttttcttt cagctcttcg atttccatct gcaacagagt gatggtatca
    39421 atcgcctgct gtacttttgc ttccagttta tcaaacactt ctaatgacat tgtcatacct
    39481 ctcctgaatt gcaaggcgtt gatggataaa aaatcctcgt cccgattacc ggtgacgcct
    39541 taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc atcgctgtcc
    39601 agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct agtgacataa
    39661 tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca aacatgcatc
    39721 atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacga agcacacaca
    39781 ttttaagttc gatatttctc gtttttgctc gttaacgata agttaacagc atgcctacaa
    39841 gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc aggatccgat
    39901 tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg gtaccgggtt
    39961 gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg cgtcttttgg
    40021 tcagtgggaa atcagtgtca tttggggact gggggtggca atggccatct acctgaccgc
    40081 aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt gcattgtggc tgtttgcctg
    40141 tttcgacaag cgcaaagtta ttccttttat cgtttcacaa gttgccggcg ctttctgcgc
    40201 tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc agactcatca
    40261 cattgttcgc ggcagcgttg aaagtgttga tctggctggc actttctcta cttaccctaa
    40321 tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg ctattctgat
    40381 ggggcttatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc ctttggctcc
    40441 cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat tgacaggttt
    40501 tgccatgaac ccagcgcgtg acttcggttc gaaagtcttt gcctagctgg cgggctgggg
    40561 caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc ttttcggccc
    40621 tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc gccatttgcc
    40681 ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact ccttcagaac aaaaagcttc
    40741 gctgtaatat gactacggga caattaaaca tgactgaaaa aaatatatcg ttgcgctcga
    40801 ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata tcattagcgt
    40861 gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggt tgggtagaac acgacccaat
    40921 ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag ccgatatcag
    40981 ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta ttgtctggga
    41041 aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc gtaccgcaga
    41101 aatctgcgag catttaaaac gtgacggttt agaagattat atcagcagca ataccggtct
    41161 ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc atgtggaagg
    41221 ctctcgcgag cgtgcacgtc gtggtgaatt gctgtttggt acggttgata cgtggcttat
    41281 ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct ctcgtaccat
    41341 gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc tggatattcc
    41401 gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta tacggtcaga ctaacattgg
    41461 cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc aggccgcgct
    41521 gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca ctggctgctt
    41581 tatgctgatg aacactggcg agaaagcggt gaaatcagaa aacggcctgc tgaccaccat
    41641 cgcctgcggc ccgactggcg aagtgaacta tgcgttggaa ggtgcggtgt ttatggcagg
    41701 cacatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct acgattccga
    41761 atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg catttaccgg
    41821 gctgagtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc tgactcgtgg
    41881 ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc agacgcgtga
    41941 cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc gcgtggatgg
    42001 tggcgcagta gcaaacaatt tcctgatgca gttccagtcc gatattctcg gcacccgcgt
    42061 tgagcgcccg gaagtgcgcg aagtcaccgc attgggtgcg gcctatctcg caggcctggc
    42121 ggttggcttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg agcgcgagtt
    42181 ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga aaaaagcggt
    42241 caaacgcgcg atggcgtggg aagaacacga cgagtaatgt caggcacctg aatcgattgc
    42301 ctgatgctga tcgcctgatg cgacgcttac gcgtcttatc aggcctacga gccaatgcca
    42361 ttttgtgtag gcgggataag gcgttcacgc cgcatccggc atcacgcaga ctctgaaacc
    42421 gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct acacttcgcg
    42481 ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa ttttcccgcg
    42541 tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat aaaaacaccg
    42601 cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac attgacggca
    42661 ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt ggtgaaaaag
    42721 tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa ggcacgcgca
    42781 tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat aaaggctgct
    42841 tcctcaatgc gccggatatg tatatggaga agctgattgt cgggccggga gccaaaggca
    42901 ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtagcggcg gcgctcggca
    42961 aaccgttgag cgaactgacg gtaacgattc tggctaaacc acgccacgat gccgttatcg
    43021 ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggacggcgac gttgcggcct
    43081 caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt ggtggcgcgc
    43141 cggaaggcgt ggtttctgcg gcggtgatcc gcgcattaga tggcgacatg aacggtcgtc
    43201 tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt ggcgagcagg
    43261 agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgt ctgggcgata
    43321 tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc gatctgctgg
    43381 aaggtattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc cgcggcaagt
    43441 cacgcaccat tcgccgcatt cagtccatcc actatctgga tcgcaaagac ccggaaatgc
    43501 aggtgcacat tctctgagtg atttgatcga ttgagccttc cagtccttcg ggactggaat
    43561 ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa aacatggctg
    43621 attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg tttagtctca
    43681 ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt taccaagctt ggccttgaaa
    43741 tcgacggcga acgcgtccag cgcgcctact cctatgttaa ctcgcccgat aatcccgatc
    43801 tggagtttta cctagtcacc gtccccgatg gcaaattaag cccacgattg gcggcactga
    43861 aaccgggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt ctggatgaag
    43921 taccggactg cgaaacgcta tggatgctgg caaccggtac agcgattggc ccttatttat
    43981 cgattctgca actaggcaaa gatttagatc gcttcaaaaa tctggtcctg gttcacgccg
    44041 cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa aaacgctacg
    44101 aaggcaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg tcgctcaccg
    44161 gacggatacc ggcattaatt gaaagtgggg aactggaaag cgcgattggc ctgccgatga
    44221 ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc gatacacaac
    44281 agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg ggccatatga
    44341 cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga gcaaatccgc
    44401 actcatctta ttgatcatcc cgcgatatgc cggcatcaaa cggttgataa atgagtgatg
    44461 atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca gtgtttccgg
    44521 catttctccg cgttcaatac gcagttttgt cttcgcccat tgaatatttt tcgtcagttt
    44581 atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg ttgcagggaa
    44641 gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg tcaggtgagc
    44701 gtcattatgt ctggcgagct ccagggcttt attcaccagt aaggcatctt cttcattccc
    44761 ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa gcaactccat
    44821 caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca tgagaaagtg
    44881 aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttataggc cacgctggcc
    44941 ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat aaaagactct
    45001 gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc gttatcaatc
    45061 accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac atctttcata
    45121 gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg cgccgttaag
    45181 ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt gcttaaggtg
    45241 aagagttcag aaggattttc cgggtattgt catttgcatc gtggaaataa caatcccgga
    45301 aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt cggctttata
    45361 cttcacatcc tgagtatctt taccatattt attttcgcct tgtgtgccaa caaacgcacc
    45421 aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacgcccca ccgcccattg
    45481 ccagacaccc ggtaaaatcg ctcagttacc cgccagcagc atccacgcca caatcatcag
    45541 aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacagtta ctgccgctgt
    45601 tggccagagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta gattcttacc
    45661 cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa tccagaaatc
    45721 acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca tgtaagttcc
    45781 ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat atcgttttaa
    45841 tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc tgttttttct
    45901 cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag atacggcaac
    45961 gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca gccagtttcg
    46021 agaagacttt aacagccaga atcccagcct gccactgaac gaatttcgtg ccatcgacag
    46081 cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa attaacgaga acttgtatgc
    46141 ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga cctggctacc
    46201 catccagggg ccagagcaaa aagcagcgaa agcgaaagct caggaataca tggcagcagt
    46261 gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac tgcagtcgct
    46321 actaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac tgcgttatgt
    46381 tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta agctggcgct
    46441 atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt gccgatgaat
    46501 ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt tattcgctta
    46561 taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa actgaacggc
    46621 agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc aggtgttgct
    46681 ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa gcgcgaagct
    46741 gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc cggcgcattc
    46801 accggtgaaa cttctgccgc tatgctgaaa gacatcggcg ctcagtacat catcatcggc
    46861 cactctgaac gtcgtactta ccacaaagag tctgacgaac tgattgcgaa aaaattcgcg
    46921 gtgctgaaag agcagggtct gactccggtt ctgtgcatcg gtgaaaccga agctgaaaac
    46981 gaagcgggaa aaactgaaga agtttgcgca cgtcagatcg acgcggtact gaaaactcag
    47041 ggtgctgcgg cattcgaagg tgcggttatc gcttatgaac ctgtatgggc aatcggtact
    47101 ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg tgaccacatc
    47161 gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg ctctgtaaac
    47221 gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct ggttggcggt
    47281 gcttctctga aagctgacgc tttcgcagtg atcgttaaag ctgcagaagc ggctaaacag
    47341 gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta actcctcgcc
    47401 ttaacgcaaa atctcacact catgatcctg gatttcctcg gctgaagcac ggttcagcgt
    47461 cagtagattt cgttgtgtcg ccagcaacac aaacgaatta tcactctgcc gtaccatcgc
    47521 cagcccgtag cttcccatat gttcccgcgc ctcaggcact tcttctgcaa gcatcataaa
    47581 tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt catgcccgcg
    47641 caaaccacct ggcagtggca accagcggct gctgatgttc gccagatttt tatccagttg
    47701 ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt gcgtacgccc
    47761 ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc cgtatttttt
    47821 gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg aaggatcggt
    47881 caacaaagga ctttcagtac cgttaatacg atacgttgac atcaacagat attgcagtgg
    47941 accattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg cacatggcga
    48001 aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct tacgtaatgt
    48061 atccgactct tcaccggtta atttccagta accaatacca gcagccacaa cggcgataac
    48121 tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt acctcaaaga
    48181 gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga aaccaccggg
    48241 tttgataatt atcctaggga aatcagcgtt tgctgatctg atcgaacgta ccgccgttag
    48301 caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg gtgaataact
    48361 tcagctttgg aaacgcattt tcgtactttt tcgctacttc agcgtcgcgc ggacggtagt
    48421 agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg taggcttccg
    48481 ccacctcttt ggtgcctttt ttctcgacca ctttatcgac caccgacacc gttggctctg
    48541 cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt tcattcgctg
    48601 ccagcagagc ttcgttttcc caggcgatca gtacatcgcc aatcccgcgc tcgacaaagg
    48661 tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac agtgcccgaa
    48721 caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag ccccaggcag
    48781 ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt aggcgtgatc accgaaacac
    48841 ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attgccctta cgcaccagga
    48901 aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc cactctttat
    48961 caatccgccc gcgttccgca atcgcgtcca cgtcataggc cagagccagc gtgacaacat
    49021 cagcttcaat accgttgatt accgacgtcg cttgtttacc tgaaccaccg tgtgactgac
    49081 gaatcaccac gttatcacca gtttgctgtt tccagtgggc gctgaatgcc ttgttgtact
    49141 gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata tcctttgcca
    49201 taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc atcgcccgac
    49261 tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa cattaaagaa
    49321 taaaaaaaga ttggctataa cttgcgggta tatgttgagg gattaaaaag gcggaagaaa
    49381 tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg cgaacgcctt
    49441 atccggccta caaaagtttg caaattcaat aaattgcaga attcatgtag gcctgataag
    49501 cgaagcgcat caggcatttt tgcttctgtc atcggtttca ggctaaagga atctgccttt
    49561 ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcaccttt gaacggacgc
    49621 ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctggatacct
    49681 acacaacgac cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc catacgggaa
    49741 gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt
    49801 gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac
    49861 atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt
    49921 tcgtttacca ggtcttcacg gctgaattca acttccggaa ctacaacgaa ttcacagcca
    49981 ccggcaatgg ccgcagccag ggtcagatcg ccacaataac ggcccatcac ttccaccacg
    50041 gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg
    50101 ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg
    50161 cccggcagac cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa
    50221 ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt ttttcaggtt ttcgatagcc
    50281 acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag gaacgtaccg
    50341 ccacggttga tcatgtcaga cacgctgtaa cggtctagct gtaccatacg gtcttcatac
    50401 agacccagat agccgtcata aatacccatt acttccagac cttctgtcag cgcagaacga
    50461 acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacacct
    50521 attttcttaa tcatgactac ctctgaactt tggaatgcaa aatgaaatct gttgccggaa
    50581 gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg
    50641 ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct
    50701 ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac gacggaacag
    50761 gggtcctgat ggataatcac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc
    50821 tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc
    50881 tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc
    50941 ggccaggaag tcacgatatc aataatttct tgccgttctt catcaggcaa tgcgcgatcc
    51001 agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg
    51061 attcccaatg caaacagagc atcggcgcga tgccagccgt accaagacaa ccccagcgcc
    51121 agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc
    51181 gcctggcttt gcgtccggcg caccacccac cgctgaaacg agacaaggat aatcgtacaa
    51241 attagcgcca caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat
    51301 accagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat aaacatactt
    51361 tgcgccagcg ccgcgaggga ctcagcttta ccgtgaccaa acgagtgatt atcgtcggca
    51421 ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat atccaccagc
    51481 gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta
    51541 atcagcaata gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt
    51601 ccataagatt gattcataaa tactcccgct atcaactgac gctagtataa cggaagcaaa
    51661 tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc acatcatgtg
    51721 ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg ggaacgtgag
    51781 ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac gtcacgcaac
    51841 tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg cgttaacagg
    51901 cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg ctcattcgcc
    51961 attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt gacaaggcga
    52021 tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc ctgttgcata
    52081 agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa catatggctc
    52141 tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt tatcgcctga accgacttca
    52201 gcagcgtggc ttaatgaact gactgccagc gttgaggcca tgacggcagc ggtaactatg
    52261 cgcatcattt gctcccaaaa tctttctgtc gcgattcaac gatagagagt ttacgattca
    52321 ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag tcatggatta gcgacgtctg
    52381 atgacgtaat ttctgcctcg gaggtattta aacaatgaat aaaatcctgt tagttgatga
    52441 tgaccgagag ctgacttccc tattaaagga gctgctcgag atggaaggct tcaacgtgat
    52501 tgttgcccac gatggggaac aggcgcttga tcttctggac gacagcattg atttactttt
    52561 gcttgacgta atgatgccga agaaaaatgg tatcgacaca ttaaaagcac ttcgccagac
    52621 acaccagacg cctgtcatta tgttgacggc gcgcggcagt gaacttgatc gcgttctcgg
    52681 ccttgagctg ggcgcagatg actatctccc gaaaccgttt aatgatcgtg agctggtggc
    52741 acgtattcgc gcgatcctgc gccgttcgca ctggagcgag caacagcaaa acaacgacaa
    52801 cggttcaccg acactggaag ttgatgcctt agtgctgaat ccaggccgtc aggaagccag
    52861 cttcgacggg caaacgctgg agttaaccgg tactgagttt accctgctct atttgctggc
    52921 acagcatctg ggtcaggtgg tttcccgtga acatttaagc caggaagtgt tgggcaaacg
    52981 cctgacgcct ttcgaccgcg ctattgatat gcacatttcc aacctgcgtc gtaaactgcc
    53041 ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt ggtcgcggct atctgatggt
    53101 ttctgcttca tgataggcag cttaaccgcg cgcatcttcg ccatcttctg gctgacgctg
    53161 gcgctggtgt tgatgttggt tttgatgtta cccaagctcg attcacgcca gatgaccaag
    53221 cttctggata gcgaacagcg tcaggggctg atgattgagc agcatgtcga agcggagctg
    53281 gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc tgttccgggc gattgataag
    53341 tgggcaccgc caggacagcg tttgttattg gtgaccaccg aaggccgcgt gatcggcgct
    53401 gaacgcagcg aaatgcagat cattcgtaac tttattggtc aggccgataa cgccgatcat
    53461 ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc cgttctccgt gcgtgatggc
    53521 gaagataatt accaacttta tctgattcgt ccggccagca gttctcaatc cgatttcatt
    53581 aacttactgt ttgaccgccc gctattactg ctgattgtca ccatgttggt cagtacgccg
    53641 ctgctgttgt ggttggcctg gagtctggca aaaccggcgc gtaagctgaa aaacgctgcc
    53701 gatgaagttg cccagggaaa cttacgccag cacccggaac tggaagcggg gccacaggaa
    53761 ttccttgccg caggtgccag ttttaaccag atggtcaccg cgctggagcg catgatgacc
    53821 tctcagcagc gtctgctttc tgatatctct cacgagctgc gcaccccgct gacgcgtctg
    53881 caactgggta cggcgttact gcgccgtcgt agcggtgaaa gcaaggaact cgagcgtatt
    53941 gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc tgttggtgat gtcacgtaat
    54001 cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca accagttgtg gagtgaagtg
    54061 ctggataacg cggcgttcga agccgagcaa atgggcaagt cgttgacagt taacttcccg
    54121 cctgggccgt ggccgctgta cggcaatccg aacgccctgg aaagtgcgct ggaaaacatt
    54181 gttcgtaatg ctctgcgtta ttcccatacg aagattgaag tgggctttgc ggtagataaa
    54241 gacggtatca ccattacggt ggacgacgat ggtcctggcg ttagcccgga agatcgcgaa
    54301 cagattttcc gtccgttcta tcggaccgat gaagcacgcg atcgtgaatc tggcggtaca
    54361 ggattggggc tggcgattgt tgaaaccgcc attcagcagc atcgtggctg ggtgaaggca
    54421 gaagacagcc cgctgggcgg tttacggctg gtgatttggt tgccgctgta taagcggagt
    54481 taaactccgc atttgtaggc aggataaggc gtttacgccg catccggcat ttgagcagga
    54541 tgcctgatgc gacgctgata gcgtcttatc aggcctacac tccgggggtt attttcccca
    54601 caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg cgcttttgca tcgtccgcgt
    54661 ccagcttttc gataacccgg cggcggagag taaacggtga tactgatcgt catcaaacgg
    54721 catatgccat gcgatggcgg cagcctcctg cacggtcaca tcgctgacac gggaaaccaa
    54781 ctccagcggc gcgtccgcag acactttccc tggcgctatc acgctataca accaccccac
    54841 cttacctgtg ttttgcatca actgcgctat atcgctgata tcaaaatggt aattgagttt
    54901 atagcaaggc gagcgcggct ggctgacctg aattaatgcc tctccccagc ggaaaatatc
    54961 gcccatatga acattacttt ccgtgagacc gtcggttgag agattttcgc caaacgcagg
    55021 cgcaacaaac aactccgcct gttccggaaa ttcccgcacc cagtagagat agtgctcacg
    55081 aggataatga cacagcgctc tgtctggccc gccgtgaacc tttttctccg cctgctcgtc
    55141 accttccagc cccagctccg tcagcatcaa ctcaccatca acctggattt tggcaattgc
    55201 gctgggtttg ccttcgggat aagcctgaat cttgcctgtg tatacatcaa ccggatatcg
    55261 catctgttcc ctctccacga gaaatttccc cattagacca tatttatttt atgtcagccg
    55321 tgctgttttc gcggtaagta tccaggcagg aactcaatag caaaccggac gaacgttagt
    55381 tcatccggct gtggggatta aggaagcagg tttatttcac cgtacctaaa atttcgattt
    55441 tcgctgctct ggctttgact ttacgtgacc agatagaggt aagaattggc accagaatcg
    55501 aagtcacaat gaccgccgtc gctaccagcg aagttgctgc cggagccatc ggtttaaacg
    55561 caggcaccat ttctgcaatc agcacaggtg tcgctaccgc ggcccctgcg gaactggaag
    55621 cggcaattcc ggcagtgcca tcgccaccgc caatcaattt atcggcgata atcaacggaa
    55681 taccggtcac gataattatt gccacaccca acaggatccc cagcaaacca gtctgggcaa
    55741 tcacagtcaa atcaatggta ttgcccagcg cgaaggcaaa gaatggaatc agcgtttgca
    55801 ccgctttgct gaaaaattct cgtaattcag ggtcaagatt ccccagggca aagcccacca
    55861 ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa cgaggcaatc ccggcagtgc
    55921 ccagaataat catcgtcatg agcggcccgg actccaacga catcaacaca aatgccccag
    55981 cttcttcttt cgtaccgtac tgctgcatga tggaagcgta aagtccgccg ttggtcatat
    56041 ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa gaatccaact tcaacaccat
    56101 gttccggaat aatgcgcgag gcaattgccg caaccaccca cgcgacggca attttagtta
    56161 ccaccagcgt accggattta cgcagtaccg ttcccgtcgc gcttaatttt attgacgccc
    56221 ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc ggtaatcatc ccgttggtga
    56281 atgatccaaa atatttcccc gcccccggcg agaaggtgtg gcacagtgcg ccaaggaata
    56341 gcgggacgag catcatcccc cccgggattt tctcaatcga gcgttttatc tgcatttcca
    56401 tcacctgtcg gcatattttt aagggctgat gcgtaattcc tcaatggagc atcagtgttg
    56461 atggaaacga agatagacat tcatttaatc aataaaagtg atcaaaacca caccaattga
    56521 aacgctgttt tatttttata ggatcattgt ttttattttg aaattgatca caaaaaaacc
    56581 accgccgttg gcgatggttc attttttaag ctgatatgcg gcctatacgc ctcattgcag
    56641 caggcggcaa atgattattt tttcgccgca aaacgtgccg ctgcttcgtc ccagttcacc
    56701 acgttccaga actctttgat gtagtccgga cggcggttct ggaatttcag gtagtaagca
    56761 tgttcccaca catccaggcc cagaatcggg aagccggaag cgccagaaat agcttcaccc
    56821 atcagcggag aatcctggtt agcagtagaa accaccgcca gtttatcgcc tttcagcacc
    56881 agccatgccc agccggaacc aaagcgggaa gctgccgctt tttcaaattc tgctttgaag
    56941 ttatcaacgg agccgaagtc acgttcgata gccgctttca ggtcaccctg cagggtggtg
    57001 ccttttttca gacctttcca gaacaggctg tggttagcgt gaccgccagc gttgttgcgc
    57061 agtacggttt tcttgtctgc tggcagctgg tccagtttgg tgatcagctc ttcaaccggc
    57121 aggttggcaa attctggcag gctttccagc gccgcgttgg cgttgtttac gtaggtctga
    57181 tggtgtttgg tgtggtggat ttccatggtc tgcttatcga agtgcggttc cagggcatcg
    57241 taagcatacg gcagggatgg cagggtatag ctcatattca tctccagtat tgtcgggcgg
    57301 ccgattgtta atgccgcgta agcagttggt ttattatagt taattaaatg atattgaaaa
    57361 tgattatcaa tgccgcactt ttcgtaaggg tatggttttg caggaaaatg cccgagatgt
    57421 gaagcaaatc acccacttaa tgccgtgatt gccagtaaat cgacaacggc ggcaacaggc
    57481 gaaaggttaa tcgacagcac gatttttaca ctcatctcgt cggagatgtg aagcgacgaa
    57541 aaatgatgag gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat
    57601 cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg
    57661 ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt attctgccgt gggccatcag
    57721 cgccctgtta ctaccggatt tctgggcgta ttacagctcg tttagtctct ctacgctgct
    57781 gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat atcaactatg gcctgaccat
    57841 gcgttatctc ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg
    57901 tacgctgatg acgccaatta tcaacggcaa tttcgatgtg ctgattaaca ccgaaggcgg
    57961 acgcatgacg ttactcggcg ttctggtggc gctgattggc gtagggattg taactcgagc
    58021 cgggcagttg aaagagcgca agatgggcat taaagccgaa gagttcaatc tgaaaaaagg
    58081 gctggtgctg gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc
    58141 cgcaaaaccg atgcatgaag ccgctgccgc acttggcgtc gatccactgt atgtcgctct
    58201 gccaagctat gttgtcatca tgggcggcgg cgcgatcatt aacctcggtt tctgttttat
    58261 tcgtctggca aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct
    58321 gatcattcac aatgtgttac tctcggcact gggcgggttg atgtggtatc tgcaattctt
    58381 tttctatgcc tagggccacg cccgcattcc ggcgcagtat gactacatca gttggatgct
    58441 gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg ctggtgctga aagagtggaa
    58501 caatacagga cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc
    58561 cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc tgcgaattga gatgcgccac
    58621 tggctgggcg tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca
    58681 aagccacatt cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc
    58741 gcatgacaca ctctgacctg ccgcagatat tgattgatgg tcattccagt ctgctggcga
    58801 aattgctgac gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca
    58861 aagggacttt tcaggctagc cgccagccgg gtaatcagct tatccagcaa cgtttcgctg
    58921 gatgttggcg gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc
    58981 ccgaacagca actcagccat ttcgttagca aacggcacat gctgactact ttcatgctca
    59041 agctgaccga taacctgccg cgcctgcgcc atccccacgc tacctaagcg ccagtgtggt
    59101 tgccctgcgc tggcgctaaa tcccggaatc gctccctgcc agtcaagatt cagcttcagc
    59161 cgctccgggc aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt
    59221 ttatcgtcag catgaatata aaagagatcg ccacgggtaa tgcgataagg acgatcgttg
    59281 agtacatgca gaccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt
    59341 tcggcaaaga catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca
    59401 aaaaaatcat ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa
    59461 cgaagttgat tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag
    59521 aaagccatcc cgtccctggc gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag
    59581 cgtcgaaaag tggttactgt cgctgaatcc acagcgatag gcgatgtcag tgacgctggc
    59641 ctcgctgtgg cgtagcagat gtcgggcttt catcagtcgc aggcggttca ggtatcgctg
    59701 aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa
    59761 ttgatccgcc acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag
    59821 aagcaagttg agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag
    59881 caagagcagt aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc
    59941 cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt
    60001 aacgcgccag tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagccc
    60061 ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca ttggtcagac acagattatc
    60121 ggtatgttca tacagatgcc gatcatgatc gcgtacgaaa cagaccgtgc caccggtgat
    60181 ggtatagggc tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc
    60241 atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac
    60301 ggacgcgtta caagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg
    60361 atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac
    60421 ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg
    60481 gtgagcatca catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct
    60541 ggttgccaat ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt
    60601 agactggtcg taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg
    60661 tcgatctcgg cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca
    60721 gcctgacgct gcgcgaaatc catcgtttta acaatgggct gcatagccaa aacggttatg
    60781 tcacctggga tgtggatagc ctggaaagtg ccattcgcct tggattaaac aaggtgtgcg
    60841 aggaagggat tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc
    60901 tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg
    60961 gcctgatgcc gcaggcacaa cagcaactcg gcaaacgcga tatttatcag cgtagcggca
    61021 tccagtttct acccttcaat acgctttatc agttgcgtgc gctgacggag caacaacctg
    61081 aacttattcc acacattgct cacgctctgc tgatgccgga ttacttcagt tatcgcctga
    61141 ccggcaagat gaactgggaa tataccaatg ccaccaccac acaactggtc aatatcaata
    61201 gcgacgactg ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc
    61261 gcccgacgca tccgggtaat gtcattgggc actggatttg cccgcagggt aatgagattc
    61321 cggtggtcgc cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg
    61381 gttcacgcgc cgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga
    61441 cgccatttac caatgacacg gcgctggcag ccaacatcac caatgaaggc ggggcggaag
    61501 gtcgctatcg ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg
    61561 aacggcaaat caacgatctc ccggcgctta tcgccgcgac acaggcactt ccggcctgcc
    61621 gcttcatcat caatcccaat gacgatcgct ttattaatcc tgacgagatg tgcagcgaaa
    61681 ttcaggctgc gtgtcgggaa acggcgcaac cgatcccggg aagtgatgct gaactggcgc
    61741 gctgtatttt cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc
    61801 tgcgcggtga agatttctcg caactgcata ttgtcggcgg cggctgccag aacacgctgc
    61861 tcaaccagct atgcgccgat gcctgcggta ttcgggtgat cgccgggcct gttgaagcct
    61921 caacgctcgg caatatcggc atcgagttaa ttacgctgga tgaactcaac aatgtggatg
    61981 atttccgtca ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca
    62041 gtgaaattgc ccactatgtg gcgcagattc actctacacg acagacaaag gagctttgcg
    62101 catgaccact caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcggtggg
    62161 gattgatgtc gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg
    62221 gcagggcgat gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gggggattca
    62281 ggccacaggc aattatccgg gcaaagcgcg taatgccagt gagctacgtg ccgatctgga
    62341 acaggctatg cggctgattc cggggccgaa acggcttaat ttacatgcca tctatctgga
    62401 atcagatacg ccagtctcgc gcgaccagat caaaccagag cacttcaaaa actgggttga
    62461 atgggcgaaa gccaatcagc tcggtctgga ttttaacccc tcctgctttt cgcatccgct
    62521 aagcgccgat ggctttacgc tctcgcatcc cgacgacagc attcgccagt tctggattga
    62581 tcactgcaaa gccagccgtc gcgtttcggc ctattttggc gagcaactcg gcacaccatc
    62641 ggtgatgaac atctggatcc cggatggtat gaaagatatc accgttgacc gtctcgcccc
    62701 gcgtcagcgt ctgctggcag cactggatga ggtgatcagc gagaagctaa accctgcgca
    62761 ccatatcgac gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg
    62821 ctccaatgag ttttacatgg ggtatgccac cagccgccat gctgtatgtg ccgcagttgc
    62881 tgctgcacgt cagccgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg
    62941 aaacccaggc aattgccagt gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg
    63001 gccttgactt cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca
    63061 atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgagctg cgcaagctgg
    63121 aagcggcggg cgattacact gcgcgtctgg cactgctgga agagcagaaa tcgttgccgt
    63181 ggcaggcggt ctgggaaatg tattgccaac gtcacgatac gccagcaggt agcgaatggc
    63241 tggagagcgt gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa acactgccgg
    63301 atgcggcacg agcgccttat ccggcctacg ggtcgaatgt ccatgcgggc tgtcaccctc
    63361 accccggccc tctccctgaa agggcgaggg agaaaaacat agcctcgtgc ggcaacggag
    63421 ctgaacaccg ctccggacag tcccctcgcc cctacgggga gagggttagg gtgaggggaa
    63481 caagccgcac catgtctaaa caaggcaacc taatttacag gaacacagaa catgcaaaac
    63541 attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc ctggctgaaa
    63601 ggctgggacg agcgcaacgg cggcaacctg acgctgcgcc tggatgacgc cgatatcgcg
    63661 ctttatcacg acaacttcca cccacaaccg cgctatattc cgctcagcca acccatgcct
    63721 ttactggcaa atacgccgtt tattgtcacc ggctccggca aattcttccg taacgtccag
    63781 ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgatggcgc gggctaccat
    63841 attctctggg ggttaaccaa cgaagccgtt ccaacttctg aacttccggc tcactttctc
    63901 tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat gcactgccac
    63961 gccaccaacc tgatcgccct cacctatgta ctggaaaacg acaccgcggt ctttactcgc
    64021 caactgtggg aaggcagcac cgaatgtctg gcagtattcc cggatggcgt tggcattctg
    64081 ccgtggatgg tgcccggcac ggacgaaatc ggccaggcga ccgcacaaga gatgcaaaaa
    64141 cattcgctgg tgttgtggcc cttccacggt gtgttcggtt ccggaccgac gctggatgaa
    64201 gcctttggtt taatcgacac cgcagaaaaa tcagcacaag tattagtgaa ggtttattcg
    64261 atgggcggca tgaaacagac catcagccgt gaagagttga tagcgctcgg caagcgcttc
    64321 ggcgttacac cactagccag tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc
    64381 tttgtcatgc aggtaaaccc cgacgcccac gaagagtatc agcgtcggca taatcccatc
    64441 tggccggaac tggaagcagt gttgaaatct cacggtgtgc ataactacgc catttacctc
    64501 gacaaagcgc gtaatctgct gtttgccatg gtagagattg aatctgaaga acggtggaat
    64561 gcggtagcca gtactgatgt ttgccaacgt tggtggaaat atatgaccga tgttatgccc
    64621 gctaacccag ataacagccc ggtgagtagc gagctgcaag aagtgtttta cctgccgtaa
    64681 ttgcccaatg tgccggagat gcctccggca caatatttta aatttcgatt tgaaaaatta
    64741 aacatattac atctcccggt aagttatatt tccctgatac attgtgagta aatcacaaaa
    64801 ataatgaata acccattaat gattcatgtg gtttatttaa ataaccatta tgtgcattac
    64861 tccgcaaatc tgacctttca ctctgtctca aattgtcatt gtttaccgac atatcgcctc
    64921 tcatcactta atcgctccac cattacaatt gattaaaata tattttatgg agtccgatta
    64981 atggcagctc ttactgcaag ctgtattgac ctgaatattc agggcaatgg cgcttattcc
    65041 gttctgaa
//
LOCUS       DADMFL010000009        63382 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.009, whole genome shotgun sequence.
ACCESSION   DADMFL010000009 DADMFL010000000
VERSION     DADMFL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.009"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNZ27_000556"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNZ27_000556"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY8663017.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNZ27_000557"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNZ27_000557"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY8663018.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNZ27_000558"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNZ27_000558"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY8663019.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNZ27_000559"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNZ27_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY8663020.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNZ27_000560"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNZ27_000560"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY8663021.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQSREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNZ27_000561"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNZ27_000561"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8663022.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JNZ27_000562"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JNZ27_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY8663023.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNZ27_000563"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNZ27_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8663024.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNZ27_000564"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNZ27_000564"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY8663025.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNZ27_000565"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNZ27_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY8663026.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JNZ27_000566"
     CDS             complement(14847..15542)
                     /locus_tag="JNZ27_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8663027.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JNZ27_000567"
     CDS             complement(15665..16702)
                     /locus_tag="JNZ27_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8663028.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="JNZ27_000568"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="JNZ27_000568"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8663029.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNZ27_000569"
     CDS             complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNZ27_000569"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY8663030.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKSGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRGYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDPRSVDDLTAGY"
     gene            19423..19863
                     /locus_tag="JNZ27_000570"
     CDS             19423..19863
                     /locus_tag="JNZ27_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY8663031.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNZ27_000571"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNZ27_000571"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8663032.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNZ27_000572"
     CDS             complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNZ27_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY8663033.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNZ27_000573"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNZ27_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY8663034.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNZ27_000574"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNZ27_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY8663035.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNZ27_000575"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNZ27_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY8663036.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNZ27_000576"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNZ27_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY8663037.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNZ27_000577"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNZ27_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY8663038.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /locus_tag="JNZ27_000578"
     CDS             complement(26601..27878)
                     /locus_tag="JNZ27_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY8663039.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVKLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNZ27_000579"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNZ27_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY8663040.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNZ27_000580"
     CDS             complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNZ27_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY8663041.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="JNZ27_000581"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="JNZ27_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY8663042.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /locus_tag="JNZ27_000582"
     CDS             complement(30964..32067)
                     /locus_tag="JNZ27_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY8663043.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNZ27_000583"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNZ27_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY8663044.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNZ27_000584"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNZ27_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY8663045.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNZ27_000585"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNZ27_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY8663046.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNZ27_000586"
     CDS             complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNZ27_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY8663047.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36805..37401
                     /gene="rsmD"
                     /locus_tag="JNZ27_000587"
     CDS             36805..37401
                     /gene="rsmD"
                     /locus_tag="JNZ27_000587"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY8663048.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37391..37660
                     /locus_tag="JNZ27_000588"
     CDS             37391..37660
                     /locus_tag="JNZ27_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY8663049.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37663..38022)
                     /locus_tag="JNZ27_000589"
     CDS             complement(37663..38022)
                     /locus_tag="JNZ27_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY8663050.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38163..38789
                     /locus_tag="JNZ27_000590"
     CDS             38163..38789
                     /locus_tag="JNZ27_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY8663051.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWMRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38863..41061
                     /gene="zntA"
                     /locus_tag="JNZ27_000591"
     CDS             38863..41061
                     /gene="zntA"
                     /locus_tag="JNZ27_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY8663052.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLLDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNZ27_000592"
     CDS             complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNZ27_000592"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY8663053.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41629..42294
                     /locus_tag="JNZ27_000593"
     CDS             41629..42294
                     /locus_tag="JNZ27_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY8663054.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42367..42924
                     /gene="dcrB"
                     /locus_tag="JNZ27_000594"
     CDS             42367..42924
                     /gene="dcrB"
                     /locus_tag="JNZ27_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY8663055.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42928..44145)
                     /locus_tag="JNZ27_000595"
                     /pseudo
     CDS             complement(42928..44145)
                     /locus_tag="JNZ27_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44277..45326
                     /locus_tag="JNZ27_000596"
     CDS             44277..45326
                     /locus_tag="JNZ27_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8663056.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45381..45968
                     /gene="acpT"
                     /locus_tag="JNZ27_000597"
     CDS             45381..45968
                     /gene="acpT"
                     /locus_tag="JNZ27_000597"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY8663057.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46079..47653
                     /gene="nikA"
                     /locus_tag="JNZ27_000598"
     CDS             46079..47653
                     /gene="nikA"
                     /locus_tag="JNZ27_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY8663058.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47653..48597
                     /gene="nikB"
                     /locus_tag="JNZ27_000599"
     CDS             47653..48597
                     /gene="nikB"
                     /locus_tag="JNZ27_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY8663059.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48594..49427
                     /gene="nikC"
                     /locus_tag="JNZ27_000600"
     CDS             48594..49427
                     /gene="nikC"
                     /locus_tag="JNZ27_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY8663060.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49427..50191
                     /gene="nikD"
                     /locus_tag="JNZ27_000601"
     CDS             49427..50191
                     /gene="nikD"
                     /locus_tag="JNZ27_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY8663061.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50188..50994
                     /gene="nikE"
                     /locus_tag="JNZ27_000602"
     CDS             50188..50994
                     /gene="nikE"
                     /locus_tag="JNZ27_000602"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY8663062.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLEAPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51000..51401
                     /gene="nikR"
                     /locus_tag="JNZ27_000603"
     CDS             51000..51401
                     /gene="nikR"
                     /locus_tag="JNZ27_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY8663063.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51521..51880)
                     /locus_tag="JNZ27_000604"
     CDS             complement(51521..51880)
                     /locus_tag="JNZ27_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY8663064.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51877..52152)
                     /locus_tag="JNZ27_000605"
     CDS             complement(51877..52152)
                     /locus_tag="JNZ27_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY8663065.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52211..53335)
                     /locus_tag="JNZ27_000606"
     CDS             complement(52211..53335)
                     /locus_tag="JNZ27_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY8663066.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNZ27_000607"
     CDS             complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNZ27_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY8663067.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGIDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPFLIAIPLVMGLSILLLKKQEG"
     gene            complement(56067..57134)
                     /locus_tag="JNZ27_000608"
     CDS             complement(56067..57134)
                     /locus_tag="JNZ27_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8663068.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVSQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57500..59122)
                     /locus_tag="JNZ27_000609"
     CDS             complement(57500..59122)
                     /locus_tag="JNZ27_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8663069.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59384..60973)
                     /locus_tag="JNZ27_000610"
     CDS             complement(59384..60973)
                     /locus_tag="JNZ27_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8663070.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMSSSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61469..62388
                     /locus_tag="JNZ27_000611"
                     /pseudo
     CDS             61469..62388
                     /locus_tag="JNZ27_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(62475..62990)
                     /locus_tag="JNZ27_000612"
     CDS             complement(62475..62990)
                     /locus_tag="JNZ27_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663071.1"
                     /translation="MTKLMQFVQRCYYMTNKKMYFILILVFTLLQVCFFALWKARDGS
                     TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFF
                     TYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSI
                     PGLIINTCAPI"
     gene            complement(62944..>63382)
                     /locus_tag="JNZ27_000613"
     CDS             complement(62944..>63382)
                     /locus_tag="JNZ27_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8663072.1"
                     /translation="TISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEM
                     FAVAFKDTYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPG
                     ANFIYDVNSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT        15209 a        17149 c        15765 g        15259 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg tgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggctct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag ttgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaagccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttt tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacgcgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catggctacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36541 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36601 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36661 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36721 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36781 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36841 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36901 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36961 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    37021 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    37081 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    37141 ttcttggcgc aaaaaggcac tccgcataat atcgtgtttg tcgatccacc gttccgccgt
    37201 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37261 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37321 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37381 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37441 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37501 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37561 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37621 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37681 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg
    37741 gtactgctgg gcgtcgaggc gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37801 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37861 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37921 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37981 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    38041 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgccact
    38101 aaactggaag caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta
    38161 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38221 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38281 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38341 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38401 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38461 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38521 ttatctggat gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38581 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38641 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38701 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38761 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38821 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38881 cacggcaaga aagcccctca atttgctgcg ttcaaaccgc taaccacggt acagaacgcc
    38941 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    39001 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    39061 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    39121 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39181 gcgggctatt ccctgctcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39241 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39301 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39361 attgctcatc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39421 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39481 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39541 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39601 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39661 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39721 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39781 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39841 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39901 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39961 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    40021 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    40081 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    40141 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40201 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40261 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40321 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40381 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40441 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40501 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40561 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40621 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40681 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40741 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40801 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40861 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40921 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40981 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    41041 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    41101 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    41161 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41221 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41281 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41341 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41401 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41461 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41521 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41581 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41641 tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct tctggtgatc
    41701 acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca taccacctgg
    41761 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41821 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41881 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41941 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    42001 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    42061 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    42121 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42181 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42241 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42301 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42361 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42421 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct
    42481 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42541 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42601 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42661 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42721 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42781 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42841 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42901 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42961 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    43021 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    43081 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg
    43141 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43201 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43261 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43321 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43381 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43441 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43501 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43561 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43621 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43681 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43741 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43801 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43861 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43921 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43981 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    44041 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    44101 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    44161 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44221 atattgctgg cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    44281 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    44341 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    44401 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    44461 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    44521 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    44581 cgcgcaaact ttttaaattg caggagatgt tgccttttct taatttgcat atgtcgccgg
    44641 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    44701 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    44761 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    44821 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    44881 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    44941 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    45001 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    45061 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    45121 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    45181 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agagtgtgta
    45241 aaatctggat ggaaacgacg aaaggtggca gcaaactagc gattttactg gggccggaca
    45301 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    45361 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    45421 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    45481 ggctggcggg gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    45541 gcgaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    45601 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    45661 tgattcgccc gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    45721 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    45781 cgcgcaaaga agccatcgtt aaacagcgcg gcggcagcgc ctggcaaatc gtcagcgtag
    45841 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    45901 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    45961 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    46021 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    46081 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    46141 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    46201 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    46261 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    46321 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    46381 tgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    46441 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    46501 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    46561 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    46621 gattggcacc ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt
    46681 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    46741 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    46801 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccagaatc cggcttacca
    46861 cacccaactg tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    46921 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    46981 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    47041 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    47101 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    47161 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    47221 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    47281 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    47341 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    47401 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    47461 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    47521 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    47581 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    47641 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    47701 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    47761 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    47821 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    47881 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    47941 ttcctgcccg ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    48001 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    48061 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    48121 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    48181 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    48241 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    48301 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    48361 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    48421 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    48481 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    48541 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    48601 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    48661 tgattgcgct caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccat
    48721 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    48781 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    48841 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg
    48901 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    48961 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    49021 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    49081 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    49141 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    49201 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    49261 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    49321 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    49381 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    49441 tgaactacgt aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    49501 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    49561 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    49621 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    49681 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    49741 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    49801 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    49861 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    49921 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    49981 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    50041 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    50101 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    50161 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    50221 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    50281 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    50341 agcaccctcg cgcggctgct ggtaggctta gaggctccct cacaagggaa tattagctgg
    50401 cgcggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    50461 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    50521 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    50581 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    50641 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    50701 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    50761 cgcctgctga aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    50821 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    50881 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    50941 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    51001 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    51061 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    51121 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    51181 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    51241 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    51301 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    51361 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    51421 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    51481 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    51541 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    51601 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    51661 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    51721 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    51781 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    51841 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    51901 taaccagtca cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    51961 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    52021 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    52081 aacaggtgtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    52141 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    52201 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    52261 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    52321 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    52381 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    52441 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    52501 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    52561 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    52621 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    52681 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    52741 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    52801 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    52861 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    52921 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    52981 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    53041 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    53101 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    53161 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    53221 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    53281 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    53341 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagaaa
    53401 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    53461 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    53521 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    53581 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    53641 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    53701 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    53761 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    53821 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    53881 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    53941 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    54001 cgattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    54061 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    54121 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    54181 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    54241 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    54301 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    54361 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    54421 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    54481 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    54541 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    54601 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    54661 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    54721 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    54781 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    54841 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    54901 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    54961 caggttttgc cgcacggtga gttcgttata gagcgaaaac gactgcgaca tatagcccac
    55021 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    55081 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    55141 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    55201 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    55261 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    55321 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    55381 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    55441 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    55501 gatcagatcc cagaactggg cgcgggagag cgggtcaatc cccgttgttg gctcatcaag
    55561 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    55621 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    55681 ctcattaatt cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    55741 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    55801 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    55861 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    55921 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat
    55981 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    56041 cgggacagga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    56101 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    56161 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    56221 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    56281 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    56341 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    56401 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    56461 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    56521 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    56581 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    56641 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatcgcccct
    56701 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    56761 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    56821 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    56881 tcctgcgaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    56941 tctttcacca gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc
    57001 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    57061 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    57121 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    57181 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    57241 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg
    57301 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    57361 aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg
    57421 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    57481 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    57541 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    57601 caccagggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    57661 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    57721 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    57781 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    57841 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    57901 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    57961 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    58021 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    58081 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    58141 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    58201 attgaacgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    58261 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    58321 ttctgttcag tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct
    58381 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    58441 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    58501 ctgagctatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    58561 aattggagaa ataggttctg tcagcgaact ggggttttat aattgtttcg aatttaatat
    58621 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    58681 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    58741 aattaatatc ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa
    58801 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    58861 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    58921 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    58981 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    59041 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    59101 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    59161 taacatttta tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac
    59221 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    59281 aaagattgta gaggcgtcat cagtatatac ctctacaatc gattaagaaa acctatgaaa
    59341 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    59401 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    59461 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    59521 caaatttctg tttagttttt ttgagcgtct gtccacattt gaagatatct tcaggttttg
    59581 gtctgaaagc agtagtgtta ttaaaataat tttcatatac aggtgtgggt gctatagcat
    59641 tgcttctaat tagatggaga agatgatcat ccatgtgttg tttatgtttt ttattaaata
    59701 tatttgctaa ttcctcagga tcaattgggt ctttcatctg attaaatgga acctgcaaag
    59761 caccgagaca ataacattga ttttcttcat ttcttattat tccgtgatga ctggttaaaa
    59821 ctttagtttc tgagttataa ttacaaacat cgagcgtttt aattaagtta tacgtatcac
    59881 cggcagagag tttataattt tctagtcgtg cagtatagtt accattaaaa ttgatttcat
    59941 gatttccagc cagaacgatg acatttttat taatacggct atctttatta ccctccatat
    60001 ttcgcataga atttagcaaa gtaaggatat atttatcacc aaaaatagta ctaaatctat
    60061 cgcctgtatg atccccaagg gggatacaag gggttatcgc atcgtccttt aaaaaaacat
    60121 gttgatgcaa ctcattcaat atcatgtctc tcgtatttaa taaattgtag agcaatttat
    60181 tatttttatg tatgtatgca gatgaagaca tgtcttcgtg ttttaataat tcacataata
    60241 aactccaacc cttttccctg atgcctatat gccctgaaac gagcaatgaa taaagtacag
    60301 cgcctactct tccatctgta tcgccaaaat aagccgtgtt ggcattttgt gggttaatca
    60361 caccacattg tttataaaag tctattttag aaaacacaga tttaacatag ctatcaaact
    60421 tatccttagg aaacgtctct ttagagacat cccaacgcca gtcagcttta aaattaccgc
    60481 taagaagagt aaagaaattt tcaggtatga tagaaagccc attaacttta taaattatgt
    60541 ctgatttatc tccggagaaa ttaataaaat aataaccatc cttacactca atcttaactg
    60601 taatgggaga cgataatttt atcacatggt tattgtctgg tataattgta aagataaacg
    60661 tgttagtagt attatcggca aaactattat attcttgaat atctatagat ggctcactat
    60721 tgtaaaacca tattttctcc atctgaagac tatgttcatt taccctccag tgaccatgtt
    60781 gcaggaaata gatgatatgc atgccaaaat cgttatctat ttgcgaatca ttatccagtt
    60841 tatcaatttc aactatcttt gaacgatgtg ttttatcaac cttgctatgg tgttgatgat
    60901 tattaacttt atctgaatgc ttatctttat ggtttgaagg gatgtactta aatatattgt
    60961 caattgcttt cataaataac ccgcgatatt tttgacctgt tagtaaaaaa taaatgatac
    61021 atgtctgaaa gacatattta cgacgatata aaacaataat gcttatatgt tcgctgattt
    61081 taagcaccga gactatccgg tcttttacta aataatggaa aacaatccat ccctggaaga
    61141 caattgtaaa aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg
    61201 gccttttgac cgctaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctata
    61261 catttgtctt taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac
    61321 atttcaatat tagcagggat acctgaggag tttaaagtga acatatatat cgggtagctt
    61381 ttcaaattaa tccctttgat tataggctta atttgcatcg cgttaggtgg ctttgtgctg
    61441 gaaagttcag ggcaacgtca cgggtcatgt gctgatttct ctggcggcca tatgcctggc
    61501 attattcact accgcattta ttatcatttc gcagctcacg cgcggcgtta atacgtttta
    61561 caatacattg ttccccatta ttggctatgc ggggtcaatt atcaccatga tatggggttg
    61621 ggcactgtta gcaggtaatg atgtgatggc agacgagttt gttgccggcc atgttatttt
    61681 tggcgttggt atgattgccg catgtgtatc gacggtggca gcgtcgtccg gtcactttct
    61741 gctcattccc aaaaatgcag cggggagcaa gagcgacgga acaccggtac aggcttattc
    61801 ttcattaatc ggtaattgcc tcattgccgt tcccgtttta ctcaccctgc tcggtttcat
    61861 ttggtctatt acgctgttac atagtgccga cataactccg cattatgtcg ccttgggtta
    61921 accgcaatct gtgcctgtct aattggccta gtggccacaa tcgtccatca aacacgtaat
    61981 acgttctcat ccaaagagca ctggctgtgg tgttattggg ttatttttct cggctcagtc
    62041 acggtgctgc aggggatata cgtcttagtc agttccgatg caagcgcccg actggctccc
    62101 ggcattattc tcatttgcct cggaatgatc tgttacagca tattctcaaa agtctggcta
    62161 ctggcactgg tctggagacg tacctgttcg ttagccaaca gaataccgat gataccggta
    62221 tttacctgtc tgttttgctt gtttttgtca tcattcctcg cggaaatcgc acaaactgag
    62281 ctggactact ttattccatc tcgggttctg gttggattaa gcgcagtatg ttttacattg
    62341 ttctctatta tttcaatatt agaggccggt tctgcgaaaa aaaattaaaa tttacataat
    62401 cccagcgtta ttcatatctt aacgctggtt ttttacgaac ttagtgaaat aatgtagaat
    62461 ataaaattat aattttatat tggagcacat gtgttaatga tcaaccccgg gatactaaaa
    62521 atatagacat tattaaactt gcttagcctc gcattgcctt tacctgttaa agatgcatcg
    62581 agaaaattga gtctgaagtt ctcatccttt atctgatttt ccagtcccga aactttggcc
    62641 ttagcattac ctgagatccg tccgttacta tctattttgt aggtaaaaaa atacgaatgg
    62701 gaaaatttcc tcggtgtatc ttcatcagtt aagccaacaa tcgtaaaact accgctacca
    62761 tcttttttta aaatgactga aaggttagca gagtagtata aagaatgatc tgttttagca
    62821 tttcttgtta acgtcgacgt acattcaaga gacgttgtgc taccatcacg agccttccag
    62881 agagcaaaaa aacacacctg tagcaatgtg aaaacaagta ttaatataaa atacattttc
    62941 ttattagtca tataatagca cctctgaaca aattgcataa gtttcgtcat tagaggattt
    63001 tatacatctt gataaatacc gctgcccttc cccaagcaaa taatgcatac tatttacatc
    63061 atatataaaa tttgcacctg gcacacaggg taaatgcttt ttcgaaattt cagcggcctc
    63121 tatttgagat ttagcgaggc caatctcgtt aagattctct gaaagatata aatcacaggt
    63181 gtcaatctta cctgatttaa ccaatggata atccgttata ctgtaggtat ctttaaaagc
    63241 cacggcaaac atttcaataa taacaattac taccacaagc gaaatcaaaa tgcgaaaaag
    63301 aggaatactc cttttgcgct ctactttttc aacaacatct tcttgtaaat gagaatcatc
    63361 ataagattgt ttacttattg tt
//
LOCUS       DADMFL010000010        60673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.010, whole genome shotgun sequence.
ACCESSION   DADMFL010000010 DADMFL010000000
VERSION     DADMFL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.010"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..320
                     /locus_tag="JNZ27_000614"
     CDS             <1..320
                     /locus_tag="JNZ27_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663073.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JNZ27_000615"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JNZ27_000615"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY8663074.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JNZ27_000616"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JNZ27_000616"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY8663075.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2164..3066)
                     /locus_tag="JNZ27_000617"
     CDS             complement(2164..3066)
                     /locus_tag="JNZ27_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY8663076.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JNZ27_000618"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JNZ27_000618"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY8663077.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JNZ27_000619"
     CDS             complement(3724..4953)
                     /locus_tag="JNZ27_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8663078.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5017..5457)
                     /locus_tag="JNZ27_000620"
     CDS             complement(5017..5457)
                     /locus_tag="JNZ27_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8663079.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JNZ27_000621"
     CDS             complement(5562..6332)
                     /locus_tag="JNZ27_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8663080.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JNZ27_000622"
     CDS             complement(6329..7255)
                     /locus_tag="JNZ27_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8663081.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JNZ27_000623"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JNZ27_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY8663082.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNZ27_000624"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNZ27_000624"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY8663083.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JNZ27_000625"
     CDS             8809..11199
                     /locus_tag="JNZ27_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY8663084.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNZ27_000626"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNZ27_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="JNZ27_000627"
     CDS             12961..13308
                     /locus_tag="JNZ27_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY8663085.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="JNZ27_000628"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="JNZ27_000628"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY8663086.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="JNZ27_000629"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="JNZ27_000629"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY8663087.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="JNZ27_000630"
     CDS             complement(15128..15490)
                     /locus_tag="JNZ27_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY8663088.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNZ27_000631"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNZ27_000631"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY8663089.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JNZ27_000632"
     CDS             18618..20471
                     /locus_tag="JNZ27_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY8663090.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="JNZ27_000633"
     CDS             complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="JNZ27_000633"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY8663091.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="JNZ27_000634"
     CDS             complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="JNZ27_000634"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY8663092.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="JNZ27_000635"
     CDS             complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="JNZ27_000635"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY8663093.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="JNZ27_000636"
     CDS             complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="JNZ27_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY8663094.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28333..29706
                     /gene="aroP"
                     /locus_tag="JNZ27_000637"
     CDS             28333..29706
                     /gene="aroP"
                     /locus_tag="JNZ27_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY8663095.1"
                     /translation="MMEGQQHGEPLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="JNZ27_000638"
     CDS             complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="JNZ27_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY8663096.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="JNZ27_000639"
     CDS             complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="JNZ27_000639"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY8663097.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31239..32132
                     /gene="nadC"
                     /locus_tag="JNZ27_000640"
     CDS             31239..32132
                     /gene="nadC"
                     /locus_tag="JNZ27_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY8663098.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32335..32775
                     /gene="ppdD"
                     /locus_tag="JNZ27_000641"
     CDS             32335..32775
                     /gene="ppdD"
                     /locus_tag="JNZ27_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY8663099.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32785..34170
                     /gene="gspE"
                     /locus_tag="JNZ27_000642"
     CDS             32785..34170
                     /gene="gspE"
                     /locus_tag="JNZ27_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY8663100.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34160..35362
                     /gene="hofC"
                     /locus_tag="JNZ27_000643"
     CDS             34160..35362
                     /gene="hofC"
                     /locus_tag="JNZ27_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY8663101.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="JNZ27_000644"
     CDS             complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="JNZ27_000644"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY8663102.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36665..37285
                     /gene="coaE"
                     /locus_tag="JNZ27_000645"
     CDS             36665..37285
                     /gene="coaE"
                     /locus_tag="JNZ27_000645"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY8663103.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37285..38028
                     /gene="zapD"
                     /locus_tag="JNZ27_000646"
     CDS             37285..38028
                     /gene="zapD"
                     /locus_tag="JNZ27_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY8663104.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38038..38235
                     /gene="yacG"
                     /locus_tag="JNZ27_000647"
     CDS             38038..38235
                     /gene="yacG"
                     /locus_tag="JNZ27_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY8663105.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="JNZ27_000648"
     CDS             complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="JNZ27_000648"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY8663106.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38784..41489)
                     /gene="secA"
                     /locus_tag="JNZ27_000649"
     CDS             complement(38784..41489)
                     /gene="secA"
                     /locus_tag="JNZ27_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY8663107.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQHKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41551..42063)
                     /gene="secM"
                     /locus_tag="JNZ27_000650"
     CDS             complement(41551..42063)
                     /gene="secM"
                     /locus_tag="JNZ27_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY8663108.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="JNZ27_000651"
     CDS             complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="JNZ27_000651"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY8663109.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="JNZ27_000652"
     CDS             complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="JNZ27_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY8663110.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="JNZ27_000653"
     CDS             complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="JNZ27_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY8663111.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="JNZ27_000654"
     CDS             complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="JNZ27_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY8663112.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46615..47535)
                     /locus_tag="JNZ27_000655"
     CDS             complement(46615..47535)
                     /locus_tag="JNZ27_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8663113.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47528..49003)
                     /gene="murC"
                     /locus_tag="JNZ27_000656"
     CDS             complement(47528..49003)
                     /gene="murC"
                     /locus_tag="JNZ27_000656"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY8663114.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49057..50124)
                     /gene="murG"
                     /locus_tag="JNZ27_000657"
     CDS             complement(49057..50124)
                     /gene="murG"
                     /locus_tag="JNZ27_000657"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY8663115.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="JNZ27_000658"
     CDS             complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="JNZ27_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY8663116.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51365..52681)
                     /gene="murD"
                     /locus_tag="JNZ27_000659"
     CDS             complement(51365..52681)
                     /gene="murD"
                     /locus_tag="JNZ27_000659"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY8663117.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52684..53766)
                     /gene="mraY"
                     /locus_tag="JNZ27_000660"
     CDS             complement(52684..53766)
                     /gene="mraY"
                     /locus_tag="JNZ27_000660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY8663118.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53760..55118)
                     /gene="murF"
                     /locus_tag="JNZ27_000661"
     CDS             complement(53760..55118)
                     /gene="murF"
                     /locus_tag="JNZ27_000661"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY8663119.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTCPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55115..56602)
                     /gene="murE"
                     /locus_tag="JNZ27_000662"
     CDS             complement(55115..56602)
                     /gene="murE"
                     /locus_tag="JNZ27_000662"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY8663120.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56589..58355)
                     /locus_tag="JNZ27_000663"
     CDS             complement(56589..58355)
                     /locus_tag="JNZ27_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY8663121.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58371..58736)
                     /gene="ftsL"
                     /locus_tag="JNZ27_000664"
     CDS             complement(58371..58736)
                     /gene="ftsL"
                     /locus_tag="JNZ27_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY8663122.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58733..59674)
                     /gene="rsmH"
                     /locus_tag="JNZ27_000665"
     CDS             complement(58733..59674)
                     /gene="rsmH"
                     /locus_tag="JNZ27_000665"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY8663123.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLVPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59676..60134)
                     /gene="mraZ"
                     /locus_tag="JNZ27_000666"
     CDS             complement(59676..60134)
                     /gene="mraZ"
                     /locus_tag="JNZ27_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY8663124.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14529 a        16807 c        14920 g        14417 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg ggcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc caggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggctca tatgattcat attgccgtgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctggcc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagtc cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgtgtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcggcaggtc
    20581 ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg
    20641 cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccagcccgat
    20701 ttcacccagc agctcgccgc cgttggtacc aacaatcgca ccaccgatca cgcggtgaga
    20761 ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc
    20821 agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt
    20881 ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt
    20941 cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac
    21001 acctttgtgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac
    21061 gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc
    21121 tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc
    21181 gtaacgctgc ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc
    21241 tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg
    21301 cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc
    21361 aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat
    21421 accgccaccc attaccagca ggcgttctgg cacttctttc agttccagcg cgtcagtgga
    21481 gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc
    21541 tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag
    21601 ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc
    21661 cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt
    21721 gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc
    21781 ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca
    21841 aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca
    21901 acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat
    21961 ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca
    22021 ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg
    22081 ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac
    22141 cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    22201 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    22261 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    22321 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    22381 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    22441 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    22501 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    22561 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    22621 agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt
    22681 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    22741 ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat
    22801 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    22861 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg
    22921 gatcatcacc cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc
    22981 cacttcttcg atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg
    23041 gataccaccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    23101 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    23161 gttaacgcca aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc
    23221 gttttcagca aattcagatt tgccttccgc tttcgcagcc ggagctgctg ccggagcttc
    23281 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggcgc
    23341 tgcgccttca acttcgaaga tcataatcag cgaaccagtt ttcactttat cgccaacgtt
    23401 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc
    23461 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    23521 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    23581 tgctggtgcg ggggtcgctg ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc
    23641 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac
    23701 gttcactttg atctctttca cggtacctgc aaacggagcc ggaacttcca tagaagcctt
    23761 gtcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag
    23821 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    23881 cgcagccgcg ggtgctgctg ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    23941 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    24001 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagatg gaacttccat
    24061 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    24121 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    24181 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    24241 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    24301 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    24361 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcgatagtc gtcagccggt
    24421 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccggc
    24481 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg
    24541 ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt cacgctataa
    24601 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    24661 aggatggaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    24721 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    24781 ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    24841 tccagaccgt catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    24901 cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca
    24961 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtct
    25021 ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag
    25081 aatgggatca tcggcagatt gttcgtgctg taagaggtcg ccgctgccag ccaggaacac
    25141 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    25201 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    25261 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    25321 agacgatctt tgatcgactt gttcttcagc atcacgttca gagcacgaac gaaagcgata
    25381 gtggtagaga gctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc
    25441 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    25501 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    25561 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    25621 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    25681 ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt ttcctgcgct
    25741 ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    25801 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    25861 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    25921 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    25981 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    26041 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    26101 aaaaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    26161 tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg
    26221 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    26281 cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa
    26341 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga
    26401 ccttccagga aagcacgggc gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg
    26461 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    26521 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    26581 cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    26641 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    26701 cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    26761 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    26821 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    26881 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cagaaagctc
    26941 aataagacag gttctacgtt tagttaccgc gcttttatat gcgcttgatt tacaacatct
    27001 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    27061 acgctcacgg cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    27121 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    27181 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    27241 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    27301 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc
    27361 gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac ggatgcgttc
    27421 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    27481 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
    27541 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    27601 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc
    27661 gaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    27721 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    27781 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    27841 tcatatctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    27901 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    27961 tatgcacaaa tgtagacttt acgtaggaaa gggatttttt aacgtttatt aaactataaa
    28021 aatccgaatt gaaccgattc acttaccaat tttttgattt ttaagtcaat aaaaacgaat
    28081 ttaaattcac tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt
    28141 aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc
    28201 ttgatctgac ggaagtcttt ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata
    28261 caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc
    28321 gcacgaggtt tcatgatgga aggtcaacag cacggcgagc cgctaaagcg cggccttaaa
    28381 aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggacagggct attcctgggt
    28441 agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt
    28501 tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca
    28561 ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc
    28621 tggaactact gggtactgta cgttttagtt gccatggcag agctgactgc cgtgggtaaa
    28681 tacattcagt tctggtatcc ggaaatccca acctgggttt ctgccgccgt attctttgtg
    28741 gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga gttctggttt
    28801 gccattatca aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc
    28861 agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg
    28921 ccgcacggct tcactggact ggtgatgatg atggcgatta tcatgttctc gttcggtggt
    28981 ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa
    29041 gcaactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg
    29101 ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc
    29161 cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg
    29221 ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa
    29281 caggggaacg cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc
    29341 attctggtgt ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa
    29401 tccgcatttg gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg
    29461 atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc
    29521 ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta
    29581 ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg
    29641 ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg
    29701 cattaatctc tctacgccct cacccgtaca gggtgagggc aataatctct acaccaacgc
    29761 cccgtaaatc gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat
    29821 tgaaaccgct gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa
    29881 ctgcgccaga cgcgttaata cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa
    29941 ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg
    30001 aaccggcacc caatccagta catgaagcac tgcatcaatg ccggactgta aacgatgatg
    30061 cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc aagaacgcat accccatcag
    30121 cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg
    30181 aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc
    30241 tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc
    30301 attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat
    30361 acacagcaaa ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg
    30421 caatgcgcgt aacagtaaaa aagtcacacc catcgcaata atggtcatgc ctaacgtgcg
    30481 cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca
    30541 gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt
    30601 catgttgtct ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga
    30661 tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatcggga
    30721 tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac
    30781 gccagagtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcacgc
    30841 ccctgatact gagagactcc cgcatgccag gcacgtttat cgaaaggaac atactggact
    30901 atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca
    30961 aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg
    31021 ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca
    31081 tccaggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt
    31141 tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt
    31201 ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctacaacc
    31261 ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg
    31321 cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa
    31381 aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat
    31441 tttgtggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca
    31501 taatctggca tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg
    31561 aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc
    31621 tttcaggagt tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc
    31681 agttgttgga tacgcgcaaa accttacctg gcctgcgttc agctctgaaa tacgcggtac
    31741 tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa
    31801 accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc
    31861 cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgag gccctgaaag
    31921 ccggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca
    31981 aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc
    32041 gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac
    32101 aagcactcga cctttcaatg cgttttcgct aattgctgac agatttatcc gctccatagc
    32161 gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt
    32221 cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg tttcgttttt tgcccctcgc
    32281 ctgtgctgat ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac
    32341 aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta
    32401 agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg
    32461 ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga
    32521 ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt
    32581 tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg
    32641 ctaagcgtcg tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc
    32701 aactgcaata ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt
    32761 gatgacgcca actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt
    32821 tatcagggag tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc
    32881 ccctcacatg agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc
    32941 tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta
    33001 gctgttcagg agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg
    33061 ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc
    33121 ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca
    33181 ttaacagcca gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag
    33241 gacgggcaat tcactgtcga actggcagga aacaccgtct catttcgtat tgcgacctta
    33301 ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaagcactg
    33361 gatgttaaca cgctgggaat gcagccgtta caactggcgg actttgctca tgccttgcaa
    33421 caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac ggtcacgctt
    33481 tatagtgccc tgcaaacgct gaataccgct gacattaata tttgtagcgt cgaagatccg
    33541 gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cgggctcacc
    33601 tttcagggtg ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag
    33661 atccgcgatg gcgaaacggc agaaattgcc attaaagccg cgcaaaccgg tcacctggtg
    33721 ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg
    33781 gtcgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt
    33841 aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc agacactgta
    33901 tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt
    33961 tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt
    34021 tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg tatgcgtacg
    34081 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
    34141 cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg gcataaccgg
    34201 cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc
    34261 actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca attctgcgca
    34321 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg
    34381 gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta aacaatggca
    34441 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
    34501 attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca cgggtgaact
    34561 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat cccagcgtca
    34621 gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag cgatggcaat
    34681 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
    34741 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
    34801 cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct
    34861 gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat
    34921 gggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca
    34981 aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc
    35041 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
    35101 gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacagg
    35161 agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa
    35221 cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac
    35281 gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc atttaggcga
    35341 tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt gggagattac
    35401 aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc
    35461 agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
    35521 tcacgcgcgg tattttcaac cgggcctcgc agcggcagct tgacggtttt accttctgct
    35581 gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
    35641 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
    35701 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca
    35761 tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca
    35821 tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt
    35881 gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc
    35941 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
    36001 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
    36061 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
    36121 tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
    36181 aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa
    36241 gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc
    36301 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
    36361 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
    36421 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
    36481 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
    36541 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
    36601 ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
    36661 aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga gtaccgttgc
    36721 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
    36781 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg aaaacatgat
    36841 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga
    36901 agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca
    36961 gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctgg tagaaaactc
    37021 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
    37081 gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca
    37141 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
    37201 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
    37261 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
    37321 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
    37381 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat
    37441 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt
    37501 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
    37561 cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
    37621 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
    37681 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc
    37741 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
    37801 gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag
    37861 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
    37921 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
    37981 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg
    38041 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
    38101 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
    38161 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
    38221 gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac gcggacagcg
    38281 tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag
    38341 acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc
    38401 gaccagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc cttcccaact
    38461 ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc
    38521 aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac gcaccaccgc
    38581 ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat
    38641 gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc gaataatacc
    38701 taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg
    38761 ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta
    38821 ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc
    38881 agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa
    38941 cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc
    39001 tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac
    39061 atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg
    39121 tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc
    39181 gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct
    39241 ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc
    39301 agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa atcgttcttc
    39361 agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag
    39421 gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg
    39481 ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg
    39541 tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg
    39601 gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg cttcataccc
    39661 agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc
    39721 atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac
    39781 tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct
    39841 tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg
    39901 gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac aatatctgta
    39961 ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca
    40021 atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg
    40081 gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc
    40141 tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca
    40201 gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg
    40261 acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc
    40321 atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt
    40381 tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg
    40441 gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca
    40501 tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt
    40561 acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg
    40621 ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg
    40681 cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt
    40741 tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct
    40801 tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact
    40861 tcgtccacca gcgcatagtg cagtttatgc tgtacacgtt cttcagggct gaacgccatg
    40921 ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg
    40981 taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac agtcaggcca
    41041 aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg
    41101 gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc
    41161 agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg
    41221 ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc
    41281 acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag acgcgcacga
    41341 aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc cggttccatg
    41401 gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg
    41461 aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg cgttacggag
    41521 ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat
    41581 gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc
    41641 ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag
    41701 cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag
    41761 tgtttgcggt gccattgcga aagaaagatg acggattacc gtgcgaatag catgttgatg
    41821 ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa
    41881 ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc
    41941 gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa
    42001 taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact
    42061 cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct tatcattaaa
    42121 gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta agaaaacagc
    42181 gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc
    42241 cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc
    42301 agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca
    42361 tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga
    42421 ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg
    42481 atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc
    42541 agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca
    42601 cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg
    42661 cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc
    42721 ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc
    42781 cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag
    42841 ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc
    42901 atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg
    42961 agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac
    43021 gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc
    43081 aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg
    43141 actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt
    43201 tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc
    43261 ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc
    43321 ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc
    43381 attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg
    43441 ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc
    43501 aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat
    43561 atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat
    43621 ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac
    43681 cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat
    43741 ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta
    43801 gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatta aacccggacg
    43861 agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa
    43921 cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt
    43981 gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga atgccatacg
    44041 cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc
    44101 ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc
    44161 agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc
    44221 agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttag tgataccgct
    44281 accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac
    44341 cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc
    44401 gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa
    44461 catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa
    44521 aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc
    44581 tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag
    44641 caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt
    44701 cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc
    44761 ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc
    44821 ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc
    44881 ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg
    44941 accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca
    45001 accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat
    45061 atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgca atgccccacc
    45121 ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc
    45181 acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc
    45241 aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca
    45301 tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg gattcttgat
    45361 cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc
    45421 gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc
    45481 ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag
    45541 cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg
    45601 ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga
    45661 cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa
    45721 agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt ccgtcgcctt
    45781 gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag
    45841 ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg
    45901 ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt
    45961 ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag
    46021 gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc
    46081 cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc atacagcatt
    46141 ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct
    46201 accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat
    46261 tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt
    46321 tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccaag
    46381 atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt
    46441 gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc gctcaccaac
    46501 actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg
    46561 cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc
    46621 cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc
    46681 catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa
    46741 ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt
    46801 ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgctttca gacctgcggg
    46861 gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga
    46921 cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg
    46981 cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc
    47041 atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct
    47101 cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag
    47161 gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc
    47221 ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca tcactccgct
    47281 gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat cttcaccgcc
    47341 gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc
    47401 gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac
    47461 cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc
    47521 gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta
    47581 aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg
    47641 ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa
    47701 ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg
    47761 cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg
    47821 cgaaatcatc atacagatcg cgcgtacggg taaaacggtg cggctgaaac agcattacca
    47881 ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt
    47941 ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg
    48001 ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca
    48061 aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca
    48121 gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctgacgca
    48181 gcagcgtaaa gtgcccctgc ggaccaatct gctgataatc ttctacacgc acgtcggcat
    48241 cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat tcgcggatca
    48301 ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc agaaaattaa
    48361 taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga
    48421 tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg
    48481 cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca
    48541 gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg
    48601 cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta
    48661 actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa
    48721 tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt
    48781 tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg
    48841 gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa
    48901 taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg cgacgcattt
    48961 cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact
    49021 tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac
    49081 ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat
    49141 ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag
    49201 ctgtggctgc tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta
    49261 ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat
    49321 ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc
    49381 cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc
    49441 ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc
    49501 cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc
    49561 cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag
    49621 cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg
    49681 aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta agcccgcaat
    49741 accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg
    49801 acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc
    49861 ccgcgcctga cgccaggcgt tgaagatacg cagcggcgca gctatcagtg cttttatacc
    49921 ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa
    49981 gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag
    50041 atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat
    50101 taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga
    50161 cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc
    50221 gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc
    50281 gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa
    50341 ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg
    50401 acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc ttcgccgata
    50461 atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgaatta
    50521 cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga
    50581 tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg
    50641 tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg
    50701 aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc
    50761 accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc
    50821 atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag
    50881 ttggcgatat agcaaaacaa cgacagtttt gtcagctccg caggctggat acgcagcaaa
    50941 ccgagatcga tccaacgcga tgccccttta acagcgctac ctactaccag cacgatcatc
    51001 agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc
    51061 agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc
    51121 gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc
    51181 atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg
    51241 atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag
    51301 atccagacca agatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa
    51361 cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct
    51421 tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac
    51481 gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc acttccgggc
    51541 gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat
    51601 tcaggtaacg cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat
    51661 gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg
    51721 cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca aagcgatgcg
    51781 gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac cctgcggcat
    51841 ctgccagcgc cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct
    51901 ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat
    51961 tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa
    52021 tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc
    52081 gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata tgatcttcag
    52141 tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc
    52201 tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac gcaggcaggc
    52261 caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg
    52321 tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc
    52381 gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg
    52441 atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcagccatt
    52501 catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc aggctaggcg
    52561 gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc
    52621 aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccttgg taatcagcca
    52681 tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca
    52741 gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg
    52801 tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga
    52861 aagcgtttct accacgaaca ccccccccat aatcaccagc aggaattcct gacgtagcag
    52921 tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc ccataaagac
    52981 ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta tcgcggtaca
    53041 gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt
    53101 catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat
    53161 aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac
    53221 gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg
    53281 gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc
    53341 cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt tggtatcttt
    53401 acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac
    53461 caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg agatcacaat
    53521 cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc
    53581 aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg
    53641 cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt
    53701 cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac
    53761 taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc actacgtgaa
    53821 cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt
    53881 aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt
    53941 tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt
    54001 acatggcagg cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg
    54061 tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac attggcgttg
    54121 taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag acggccagga
    54181 acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg
    54241 gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga
    54301 acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg
    54361 atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt
    54421 gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata
    54481 ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag
    54541 ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc cgggcaagtc
    54601 agactcacag tccaggcaat ctcgccctga tggttcgcgc caagttcaat aactgcgtaa
    54661 tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg
    54721 cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg
    54781 gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg ctggcgaacc
    54841 catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg
    54901 atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca
    54961 aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc cggcgtcagt
    55021 tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg
    55081 agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc agcagacgcg
    55141 ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcctcat
    55201 ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca
    55261 ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa
    55321 tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca
    55381 cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc
    55441 caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg
    55501 ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg
    55561 cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat
    55621 cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt
    55681 taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa
    55741 tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt
    55801 tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc
    55861 ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa
    55921 gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc
    55981 ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc
    56041 cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc cccgccagct
    56101 catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact ttccccagca
    56161 gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg
    56221 ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat
    56281 tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc
    56341 tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcatctt
    56401 tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc
    56461 cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt
    56521 cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt
    56581 cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta
    56641 tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc gcccatgatg
    56701 gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg
    56761 ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc ggtataagca
    56821 atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg
    56881 gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag cgccacgctt
    56941 tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc accgggaacc
    57001 ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg
    57061 tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga gaaggtggcc
    57121 ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac
    57181 cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac
    57241 ggcatcgcta acgccagctt ggaaacaccg acgttactcg acttctgtaa taccccggtc
    57301 agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga
    57361 atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc
    57421 atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc
    57481 tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc
    57541 acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt gttaaaggcc
    57601 accgcgttgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc
    57661 aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg
    57721 tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca
    57781 acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg
    57841 ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag tttcagtttt
    57901 ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa gcgccctttc
    57961 gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc
    58021 gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc
    58081 caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg
    58141 ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc
    58201 atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg
    58261 agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga
    58321 cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc
    58381 actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc
    58441 ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca
    58501 atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta
    58561 tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt
    58621 ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg
    58681 tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt
    58741 tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca gccacctctt
    58801 cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt tttttgagct
    58861 gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa
    58921 aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg
    58981 ggaccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta
    59041 cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg
    59101 gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt
    59161 tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaacg ctcttcacca taggttttca
    59221 atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac
    59281 gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag
    59341 catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg atcttgccaa
    59401 taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg atgatggaga
    59461 agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca
    59521 gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa
    59581 aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca
    59641 gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc
    59701 tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc
    59761 caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt
    59821 gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc
    59881 tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac cgggttcatg
    59941 ctcgacagac gcgataattt ttgctcgata atttcccatt caggcagggg gtaaagcagc
    60001 aggcacgggt gatgaatgtc aatggcgcaa accatttgac cggcagcgtt ctcaagcagc
    60061 tgttcccgat aacgggtagg cactgataag cgccctttgc tgtcgagatt gactaacgtt
    60121 gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa tttcccactg
    60181 aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca taccgaggta
    60241 aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc
    60301 aaattaacgt tatgaatagc tgtaagaaaa aatccctcat cgtcatcact gttttaaaat
    60361 aactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc ggcattttag
    60421 gacatatctt ccccggttaa cagtccttgt tacgtctgtg tggcgcttga tgccagcggg
    60481 tttgcgcaaa agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa
    60541 aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag ctggtgagtt
    60601 aattaacggg cgggtttaaa accatttatc aataatagtc aggaatagtc ttatttactt
    60661 taaggatatt gat
//
LOCUS       DADMFL010000011        60057 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.011, whole genome shotgun sequence.
ACCESSION   DADMFL010000011 DADMFL010000000
VERSION     DADMFL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.011"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(61..567)
                     /gene="mug"
                     /locus_tag="JNZ27_000667"
     CDS             complement(61..567)
                     /gene="mug"
                     /locus_tag="JNZ27_000667"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY8663125.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            692..767
                     /locus_tag="JNZ27_000668"
     tRNA            692..767
                     /locus_tag="JNZ27_000668"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Ile,seq:cat)
     gene            complement(821..1567)
                     /gene="nfeF"
                     /locus_tag="JNZ27_000669"
                     /pseudo
     CDS             complement(821..1567)
                     /gene="nfeF"
                     /locus_tag="JNZ27_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            1844..2467
                     /locus_tag="JNZ27_000670"
     CDS             1844..2467
                     /locus_tag="JNZ27_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY8663126.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2621..4141)
                     /locus_tag="JNZ27_000671"
     CDS             complement(2621..4141)
                     /locus_tag="JNZ27_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8663127.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSCLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4447..5889
                     /gene="ygjG"
                     /locus_tag="JNZ27_000672"
     CDS             4447..5889
                     /gene="ygjG"
                     /locus_tag="JNZ27_000672"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY8663128.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASAFACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLDGFR
                     QLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEP
                     PLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(5931..6262)
                     /locus_tag="JNZ27_000673"
                     /pseudo
     CDS             complement(5931..6262)
                     /locus_tag="JNZ27_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            6481..7464
                     /gene="ebgR"
                     /locus_tag="JNZ27_000674"
     CDS             6481..7464
                     /gene="ebgR"
                     /locus_tag="JNZ27_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY8663129.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7646..10722
                     /gene="ebgA"
                     /locus_tag="JNZ27_000675"
                     /pseudo
     CDS             7646..10722
                     /gene="ebgA"
                     /locus_tag="JNZ27_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            10719..11168
                     /locus_tag="JNZ27_000676"
     CDS             10719..11168
                     /locus_tag="JNZ27_000676"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY8663130.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11231..12664
                     /locus_tag="JNZ27_000677"
     CDS             11231..12664
                     /locus_tag="JNZ27_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8663131.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12798..13869
                     /gene="ygjJ"
                     /locus_tag="JNZ27_000678"
                     /pseudo
     CDS             12798..13869
                     /gene="ygjJ"
                     /locus_tag="JNZ27_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            13886..16237
                     /gene="ygjK"
                     /locus_tag="JNZ27_000679"
     CDS             13886..16237
                     /gene="ygjK"
                     /locus_tag="JNZ27_000679"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY8663132.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            16463..18481
                     /locus_tag="JNZ27_000680"
     CDS             16463..18481
                     /locus_tag="JNZ27_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY8663133.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWDGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18526..18942)
                     /gene="higA"
                     /locus_tag="JNZ27_000681"
     CDS             complement(18526..18942)
                     /gene="higA"
                     /locus_tag="JNZ27_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY8663134.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18939..19253)
                     /locus_tag="JNZ27_000682"
     CDS             complement(18939..19253)
                     /locus_tag="JNZ27_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY8663135.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(19538..20674)
                     /gene="rlmG"
                     /locus_tag="JNZ27_000683"
     CDS             complement(19538..20674)
                     /gene="rlmG"
                     /locus_tag="JNZ27_000683"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY8663136.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            20759..21262
                     /locus_tag="JNZ27_000684"
     CDS             20759..21262
                     /locus_tag="JNZ27_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8663137.1"
                     /translation="MSNLTYLQGYPELLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMELQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            21339..22031
                     /locus_tag="JNZ27_000685"
     CDS             21339..22031
                     /locus_tag="JNZ27_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY8663138.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            22092..23108
                     /locus_tag="JNZ27_000686"
     CDS             22092..23108
                     /locus_tag="JNZ27_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8663139.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            23379..24344
                     /gene="alx"
                     /locus_tag="JNZ27_000687"
     CDS             23379..24344
                     /gene="alx"
                     /locus_tag="JNZ27_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY8663140.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            24940..26184
                     /gene="sstT"
                     /locus_tag="JNZ27_000688"
     CDS             24940..26184
                     /gene="sstT"
                     /locus_tag="JNZ27_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY8663141.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(26189..26740)
                     /locus_tag="JNZ27_000689"
     CDS             complement(26189..26740)
                     /locus_tag="JNZ27_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY8663142.1"
                     /translation="MTAYWLVQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(26823..28310)
                     /locus_tag="JNZ27_000690"
     CDS             complement(26823..28310)
                     /locus_tag="JNZ27_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY8663143.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(28325..29737)
                     /gene="uxaC"
                     /locus_tag="JNZ27_000691"
     CDS             complement(28325..29737)
                     /gene="uxaC"
                     /locus_tag="JNZ27_000691"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY8663144.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            30220..31518
                     /locus_tag="JNZ27_000692"
     CDS             30220..31518
                     /locus_tag="JNZ27_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663145.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            complement(31606..32238)
                     /locus_tag="JNZ27_000693"
     CDS             complement(31606..32238)
                     /locus_tag="JNZ27_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY8663146.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(32691..35375)
                     /locus_tag="JNZ27_000694"
     CDS             complement(32691..35375)
                     /locus_tag="JNZ27_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663147.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTCT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(35467..35967)
                     /locus_tag="JNZ27_000695"
     CDS             complement(35467..35967)
                     /locus_tag="JNZ27_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663148.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(35997..36713)
                     /locus_tag="JNZ27_000696"
     CDS             complement(35997..36713)
                     /locus_tag="JNZ27_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8663149.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            37009..37785
                     /gene="exuR"
                     /locus_tag="JNZ27_000697"
     CDS             37009..37785
                     /gene="exuR"
                     /locus_tag="JNZ27_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY8663150.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLRFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            38130..38792
                     /locus_tag="JNZ27_000698"
     CDS             38130..38792
                     /locus_tag="JNZ27_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8663151.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            38796..39179
                     /gene="mzrA"
                     /locus_tag="JNZ27_000699"
     CDS             38796..39179
                     /gene="mzrA"
                     /locus_tag="JNZ27_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY8663152.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            39326..39680
                     /locus_tag="JNZ27_000700"
                     /pseudo
     CDS             39326..39680
                     /locus_tag="JNZ27_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            39718..40023
                     /locus_tag="JNZ27_000701"
     CDS             39718..40023
                     /locus_tag="JNZ27_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8663153.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            40026..40430
                     /locus_tag="JNZ27_000702"
     CDS             40026..40430
                     /locus_tag="JNZ27_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663154.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            40420..40719
                     /locus_tag="JNZ27_000703"
     CDS             40420..40719
                     /locus_tag="JNZ27_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663155.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            40815..41297
                     /locus_tag="JNZ27_000704"
     CDS             40815..41297
                     /locus_tag="JNZ27_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8663156.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            41367..42353
                     /locus_tag="JNZ27_000705"
     CDS             41367..42353
                     /locus_tag="JNZ27_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY8663157.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            42646..43014
                     /locus_tag="JNZ27_000706"
     CDS             42646..43014
                     /locus_tag="JNZ27_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY8663158.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(43221..44117)
                     /locus_tag="JNZ27_000707"
     CDS             complement(43221..44117)
                     /locus_tag="JNZ27_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8663159.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            44222..44923
                     /locus_tag="JNZ27_000708"
     CDS             44222..44923
                     /locus_tag="JNZ27_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8663160.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            44946..45110
                     /locus_tag="JNZ27_000709"
     CDS             44946..45110
                     /locus_tag="JNZ27_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663161.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(45322..46614)
                     /locus_tag="JNZ27_000710"
     CDS             complement(45322..46614)
                     /locus_tag="JNZ27_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY8663162.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(46642..47973)
                     /locus_tag="JNZ27_000711"
     CDS             complement(46642..47973)
                     /locus_tag="JNZ27_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY8663163.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMMISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(48248..49612)
                     /gene="tdcG"
                     /locus_tag="JNZ27_000712"
     CDS             complement(48248..49612)
                     /gene="tdcG"
                     /locus_tag="JNZ27_000712"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8663164.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(49684..50073)
                     /locus_tag="JNZ27_000713"
     CDS             complement(49684..50073)
                     /locus_tag="JNZ27_000713"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY8663165.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(50087..52380)
                     /gene="tdcE"
                     /locus_tag="JNZ27_000714"
                     /pseudo
     CDS             complement(50087..52380)
                     /gene="tdcE"
                     /locus_tag="JNZ27_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(52414..53622)
                     /gene="tdcD"
                     /locus_tag="JNZ27_000715"
     CDS             complement(52414..53622)
                     /gene="tdcD"
                     /locus_tag="JNZ27_000715"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY8663166.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(53648..54979)
                     /gene="tdcC"
                     /locus_tag="JNZ27_000716"
     CDS             complement(53648..54979)
                     /gene="tdcC"
                     /locus_tag="JNZ27_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY8663167.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(55001..55990)
                     /gene="tdcB"
                     /locus_tag="JNZ27_000717"
     CDS             complement(55001..55990)
                     /gene="tdcB"
                     /locus_tag="JNZ27_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY8663168.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(56089..57027)
                     /gene="tdcA"
                     /locus_tag="JNZ27_000718"
     CDS             complement(56089..57027)
                     /gene="tdcA"
                     /locus_tag="JNZ27_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY8663169.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            57217..57561
                     /gene="tdcR"
                     /locus_tag="JNZ27_000719"
     CDS             57217..57561
                     /gene="tdcR"
                     /locus_tag="JNZ27_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY8663170.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            57817..58356
                     /locus_tag="JNZ27_000720"
     CDS             57817..58356
                     /locus_tag="JNZ27_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663171.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            58378..59544
                     /locus_tag="JNZ27_000721"
     CDS             58378..59544
                     /locus_tag="JNZ27_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HAY8663172.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT        15053 a        15417 c        14677 g        14910 t
ORIGIN      
        1 ccgaaaaaga gccagtcagt tgcttaactg actggcatta caccttttcg gtggcgtttt
       61 ttatcgccca cgcactacca gcgcctggtc cagctcgcga tacgcttcaa ccagtttctc
      121 cagtgaaacg cgacttaaac cgctgggatt tggcagcacc caaatctgcg tcgaaccaat
      181 ggtgagcgtt tgtttccccc actgtgcacc gcgctggctg aatccctgtt catatgcttg
      241 tttgcccaga atcgccaacg cctgcggctg ataatcttca attttttcaa tcagcttacg
      301 cccacctgcg tgcagctcct gctttgaaac ttcattggct tgcaccgttg gacggtctac
      361 cagtttggtg acgccacaac gataatccag cagatgctgt gcctcctgcg gcttcaactg
      421 acggtcggta aacccggcct gatatatcac cttccagaag cgatttgccg gatgagcaaa
      481 gggaaaacca gtcccggcgg atgaaagccc agggttgata ccgcaaaaca cgacccgtaa
      541 ccctggagcc aaaatatcct caaccatatt tactcctttc taacatcacc aggaaaagta
      601 taacggattg aaaatacatt gtttataaaa acagcaggcg cgcggtaatg gctggattgc
      661 gacacggagt tactttataa tccgctacca tggcccctta gctcagtggt tagagcaggc
      721 gactcataat cgcttggtcg ctggttcaag tccagcaggg gccaccagat atagcaaagg
      781 ctgacgagaa atcgtcagcc tttttctttt tatatatcag ttactttgcg tgccagtaag
      841 ccgctgcacg tacccgctgc gggtcatact gttccgcttc aaagcggcgg cttaaattct
      901 taacgacttt accttcgccg gttatccaga tgaagtaatc atctggcagg gatttgcatc
      961 tgcgccagac gcgcatctac cgcctgctca tcatgtgcca gccattcgat attaaaacca
     1021 tcaaggtgcg cgagataatc ctgacaggcg ttatcccgca cgctaaccag cgcactaact
     1081 tgcggtttaa cggcaagttt gctcaacgtt tccaggcggc ggcgcagtgc gggcattccg
     1141 gattcatcgc agacatacag ctgatacgcg taatcttccg gcaccaccag cgaaccgcgc
     1201 ggacctgcca ccgtaagttt atcgcccagc cgctggcgac cccaccgtcg tgaataaaga
     1261 aatcgatcgc cagttcatgg cgtagttcgt catacagtgg cgtatagtca cgcgacggtg
     1321 ggcgtggtcc ttccggccag acgatgccct cttccgttac cgttggcggc acaaagtgag
     1381 cgtcaggttg aggaaagaag agtttgctgt gatcgtcaaa gccgcgcgat gtaaaaccgt
     1441 ccaacgcctc gccgccaagg acaatgcgct gaaaaccggc gctgatgcgc tcaacgcgta
     1501 gcacagtcag ttcacggaag cgcagatcat tgcgaacgcg ctgcgggtag cggggggtgt
     1561 tattcattgt tatcgccttc gtgatggtaa tcagatatat ctaaataaaa ttcgcaaatg
     1621 ataatgattg ttaatcatga taaatgcaag cgatttgtag aactgatatg tctgaagtat
     1681 gtgaaggcat tcagtgccat agcaatgctg ccggatgcgg cgtgaacgtc ttatccggcc
     1741 tagatgtgag gtgcttccac gtttaaaaat catttttgta cttgcagtaa tgctcacatc
     1801 agatataaat tagatatatc taattaagca aaaggaggct gatatgagcc atcatcacga
     1861 agggtgttgt aaacatgaag gccagccacg ccatgagggc tgctgcaaag gtgagaagtc
     1921 agaacacgag cactgcggac acggtcacca gcatgaacac ggtcaatgtt gcggtggtcg
     1981 ccacggtcgc ggcggcggtc gtcggcaacg tttctttggt cacggtgaat tgcgtctggt
     2041 gattctggat attctctcgc gcgatgacag ccacggttac gaattgatta aagcgattga
     2101 gaatctaacc caggggaatt acaccccaag cccgggcgtc atctacccga cgctggattt
     2161 tctgcaggag cagtcgctga ttaccatccg cgaagaggaa ggtggtaaga agcagattgc
     2221 gctgaccgaa caaggcgcgc agtggctgga agaaaaccgc gaacaggtgg agatgattga
     2281 agaacgcatc aaagcgcgtt gcgttggcgc ggcgctgcgc cagaacccgc aaatgaagcg
     2341 ggcgctggat aattttaaag cggtgctgga tttacgcgtc aaccagagcg atatcagtga
     2401 tgcacaaata aaaaagatca ttgcggtgat cgaccgcgcc gcttttgata ttacgcaact
     2461 ggattaatcg ccgcatccgc cagtggcgcg gtgcaattgc cggatgcgac gcttgacgcg
     2521 ccttatccgg cctacacccg ctacacaccc cgcaggcctg ataagatgcg ccagcatcgc
     2581 atcaggtatt gtgctccaac cgccggatcc ggcataccga ttaatgcagc accgtcaccg
     2641 cgtcttccag tcggctggcg cggtgtttca ccatcgccga cacctgcgca ctctcttcca
     2701 ccagctcggc atttttctgg gtgatcaggt taagctcatc cactgcacgg gtcaggcagg
     2761 aaagcccatc ggcctgttcc agcgttgaat ggctaatctg ggcgatcaac tgggtgacgt
     2821 ttttcacctg tgccacaata tcttccatcg tccgtccggc ggcgtgtacc tgctgcgaac
     2881 cggattgcac cttatcagca ctggcatcaa tcagcttgcg aatatcgttg gcagcattag
     2941 cgctgcggct ggctaaatga cgcacttccc ctgccaccac tgcaaaacct ttgccctgtt
     3001 cgccggcacg cgccgcttcc accgccgcat tcagggccag aatattggtc tgaaacgcaa
     3061 tatcgttaat cagcgaagta atggtgccaa tgcgctgggt actgtcggcg atatcgtcca
     3121 ttgtcttgat caccgtggtc atcgcctccc caccctgcac tgccgcatta ctggcggtga
     3181 tagaaagttt atcggcagcc gacgccgtgg cagagttctg tttcaccgac gccgccattt
     3241 ggttcatggt cgccaccgtt tgctgaacgt tatcaactgt ctgctgggta tgttcgttca
     3301 gttcatcggt gcctttcgcc agcgtctcac tgccatttct gacactggac acctggcttg
     3361 agacatcgtt aattagccaa cggcacatca ggccaagctg ccctaccgcg cgtaatgtca
     3421 gccccagctc atcgctgcga ttcaggtgct caacactatt acgctctccg gtcgccacct
     3481 tcagtgcctg acgggcgaca ttttctatcg ggcgcacaat ctgccattca aaacaagcgc
     3541 ttgccaacaa taccactaac acacagagga aatacgtcac caccggggca gcaacaaacc
     3601 aaagcatggc cgccagcaag ataaacatca gggtcatcac tccacgcgtc cgccagcgaa
     3661 gcggtaatga aggcagttta cccagccagc ctttacgcac caccaggcct ttatgaatac
     3721 gcttactggt acgtccggag ttcaacgctt tgtacagcgg ctccaccgcc gcaatctctt
     3781 catccgtcgc ccgggtacga atcgacatat agccactgat ttttccctcg cgcaccatcg
     3841 gtaccgcatt ggcccgcacc caataatggt cgccattttt gcggcgattt ttcacgatgc
     3901 cgctccaggg ctcccctttt ttcagggtga accacatatc cgcaaacgcc gcttttggca
     3961 tatccgggtg acgcaccatg ttgtgcggct gcccttgtaa ctcttgcaag gtaaagccgc
     4021 tcacctgcac aaaagtgtca ttagcatgag tgatatagct ttgcagatcg gtagtggaca
     4081 tcagagtggt atcgtccgcc agcggggtat tttgctgggt gacatacgga tgagaagaca
     4141 tgttcgcgtc ctgtgcaggt tatatggttg ttaacttctt gtcagagttt atgtcggccc
     4201 cgctgcggtt atctttaacc gattaatttg atttagatcg caatttgcga ttaaagcaca
     4261 aatctaattc tttgatttaa aatactttca ctctgttact atacgaaaac gttaattatc
     4321 ttgcccaaaa atcaggcaat tattgccctg aaaacgtgca tttgcgcagc aatcatcaaa
     4381 tccatacccg acaaaaccgt gcaaaataac aacaaatgtt aacggatagc attaaatatt
     4441 gcacaaatga taaccgcatt tgtgtttatc ccgattttcg cgatcgcagc cggagtggcg
     4501 caatccctgc aatacttaaa tcggtatcat gtgatacgcg agcctccgga gcatattttg
     4561 aacaggttac cttcgagcgc atcggctttc gcgtgcagcg cccacgccct gaatctcatt
     4621 gagaagcgaa cgctggatca tgaggagatg aaagcactta accgagaggt gattgaatac
     4681 ttcaaagagc atgtcaatcc ggggttttta gagtatcgca aatctgttac cgccggcggg
     4741 gattacggag ccgtagagtg gcaagcggga ggtttaaata cgcttgtcga cacccaggga
     4801 caggagttta tcgactgcct gggaggtttt ggaattttca acgtggggca ccgtaatcca
     4861 gttgtggttt ccgccgtaca gaatcaactt gcgaaacaac cactgcacag ccaggagcta
     4921 ctcgatccgc tacgggctat gctggcgaaa acccttgctg cgctaacgcc cggtaaactg
     4981 aaatacagct tcttctgtaa tagcggcacc gagtccgttg aagcggcgct gaagctggcg
     5041 aaggcttatc agtcgccgcg cggcaaattt acttttattg ccaccagcgg cgcgttccac
     5101 ggtaaatcac ttggcgcgct gtcggccacg gcgaaatcga ccttccgcaa accgtttatg
     5161 ccactgctgc cagggttccg tcatgtgccg tttggcaata tcgaagccat gcgcacggct
     5221 cttaatgagt gcaaaaaaac cggtgatgat gtggctgcgg tgatcctcga accgattcag
     5281 ggcgaaggtg gcgtaattct gccgccgccg ggctatctca ccgccgtgcg taagctgtgc
     5341 gatgagttcg gcgcactgat gatcctcgat gaagtacaaa cgggcatggg gcgcacgggc
     5401 aagatgtttg cctgcgaaca tgagaacgtt cagccggaca ttctgtgtct ggcaaaagcc
     5461 ctcggcggcg gcgtgatgcc gattggcgcg accatcgcca ctgaagaggt gttttcagtt
     5521 ctgttcgaca acccattcct gcataccacc acctttggcg gcaacccgct ggcctgtgcg
     5581 gcggcgctgg cgaccatcaa tgtgttgctg gacggtttcc gtcaactggc gcgggaatat
     5641 cccgatctgg tacaggaagc gcgtggtaaa gggatgttga tggcgattga gtttgttgat
     5701 aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtact ggtggccgga
     5761 acgctcaata acgccaaaac gatccgcatt gaaccgccac tgacactgac cattgaacag
     5821 tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa
     5881 gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca tccgatcttt ttatacgacg
     5941 cgtacgcccg caggcatcat ccgctccggc gttaacaaca cgctttcact gccgtcatcg
     6001 gtttccgcac acaacagcat gcattccgac gtttcaccac gcattttcgc tttttgcaga
     6061 ttgcataaga ccaccaccgt tttccccatc agctcctctt cgctgtagta cggcaccaag
     6121 ctggtcacgg tttgcagtgt tttttcccca catcaacctg tacgatgtac agcttgtcgg
     6181 cgttttcatg gcgtttcact tccacaatct ttccgacgcg catttccaga cgtgcaaaat
     6241 cagcgtaagc cacggtttcc attgcttcct cccttcgggt aaaattttac taaactatag
     6301 caaaagtttt tctcaatcct gtaggctaaa aatggagaat gcaggcgtga tcacattcct
     6361 aagccgctgt gttaccgtta cagcgtcaaa gaaacgcgct ttatttactg aaaacaggtg
     6421 acccgataag cacttcctct acaatggagg cgcacatcag ggaaagtaaa aaaggtaaac
     6481 atggcaacat taaaagacat cgcaatcgaa gctggcgtat ccctggcgac agtatccagg
     6541 gtcttaaatg acgatccgac attgaatgtg aaagaagaga cgaaacatcg cattctcgag
     6601 atcgccgaaa agctggagta caagaccagt agcgcccgta aactacagac aggcgcagtc
     6661 aaccaacacc atattctggc tatctacagc taccagcagg agctggagat caacgatcct
     6721 tactatctgg cgatccgcca cggcattgaa actcagtgcg aaaagctggc gatcgaactg
     6781 accaactgtt atgaacacaa cggattacca gacattaaaa acgtcaccgg tattttaatt
     6841 gtcggcaaac ccacgcccgc cctgcgcgcc gctgccagcg cgttgaccga caatatctgt
     6901 tttatcgact ttcacgaacc cggcagcggt tacgatgcgg tggatatcga tctggcacgc
     6961 atcagtaaag aaatcatcga cttctatatc aaccagggcg ttaatcgtat tggttttatt
     7021 ggcggtgaag atgagcctgg caaggcggat attcgtgagg tcgcctttgc ggaatatggc
     7081 cgactgaaac aagtggtacg cgaagaggat atctggcgcg gcggtttttc cagttcgtcg
     7141 ggttatgaac tggcaaaaca aatgctggcg cgggaagact atccgaaggc actgtttgtt
     7201 gcttccgatt ccattgctat cggcgtactg cgggcaattc atgaacgtgg cctgaacatc
     7261 ccacaagata tttcgcttat cagcgttaac gatatcccca ccgcgcgatt tacctttccg
     7321 ccgctctcca ccgtgcgcat ccattccgag atgatgggaa gtcagggcgt taacctggtg
     7381 tatgaaaaag cccgcgatgg tcgcgcgctg ccgctgctag tcttcgttcc cagcaaatta
     7441 aaactgcgcg gcacgacccg ttaaatcccc ttacacactg tccggcaatc atttttgccg
     7501 gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa atgcatttac ctgctacttt
     7561 tagtaaaaat tttactaacc ccccagcaat tacacaaact accatcacca tgaatggttc
     7621 cgatttctct ctaccgggag gccctatgaa tcgctgggaa aacattcagc tcacccacga
     7681 aaaccgactt gcgccgcgtg cgtacttttt ttcatatgat tctgttgcgc aagcgcgtac
     7741 ctttgcccgc gaaaccagca gcctgtttct gcccttaagc ggtcagtgga atttgaccat
     7801 ccgctgcaag taccagaagc cttcacctct gagttaatgg ctgactgggg gcatattacc
     7861 gtccccgcca tgtggcaaat ggaaggtcac ggcaaactgc aatataccga cgaaggtttt
     7921 ccgttcccca tcgatgtgcc gtttgtcccc agcgataacc caaccggtgc ctatcaacgt
     7981 attttcaccc tcagcgacgg ctggcagggt aaacagacgc tgattaaatt tgacggcgtc
     8041 gaaacctatt ttgaagtcta cgttaacggt cagtatgtgg gtttcagcaa gggcagtcgc
     8101 ctgaccgcag agtttgacat cagcgcgatg gttaaaaccg gcgacaacct gttgtgtgtg
     8161 cgcgtgatgc agtgggcgga ctctacctac gtggaagacc aggatatgtg gtggtcggcg
     8221 gggatcttcc gcgatgttta tctggtcgga aaacacctaa cgcatattaa cgatttcact
     8281 gtgcgtaccg actttgacga agcctattgc gatgccacgc tttcctgcga agtggtgctg
     8341 gaaaatctcg ccgcctcccc tgtcgtcacg acgctggaat ataccctgtt tgatggcgaa
     8401 cgcgtggtgc acagcagcgc cattgatcat ttggcaattg aaaaactgac cagcgccagc
     8461 tttgctttta ctgtcgaaca gccgcagcaa tggtcagcag aatcccctta tctttaccat
     8521 ctggtcatga cgctgaaaga cgccaacggc aacgttctgg aagtggtgcc acaacgcgtt
     8581 ggcttccgtg atatcaaagt gcgcgacggt ctgttctgga tcaataaccg ttatgtgatg
     8641 ctgcacgacg tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt tggaatggat
     8701 cgcgtcgaga aagatctcca gttgatgaag cagcacaata tcaactccgt gcgtaccgct
     8761 tactacccga acgatccgcg tttttacgaa ttgtgtgata tctacggcct gtttgtgatg
     8821 gcggaaaccg acgtcgaatc gcacggcttt gctaatgtcg gcgatatcag ccgtattacc
     8881 gacgatccgc agtgggaaaa agtctacgtc gagcgcattg ttcgccatat ccacgcacag
     8941 aaaaaccatc cgtcgatcat catctggtcg ctgggcaatg aatccggcta tggctgtaac
     9001 atccgcgcga tgtaccatgc ggcgaaagcg ctggatgaca cgcgactggt gcattacgaa
     9061 gaagatcgcg atgctgaagt ggtcgatatt atttccacca tgtacacccg cgtgccgctg
     9121 atgaatgagt ttggtgaata cccgcatccg aagccgcgca tcatctgtga atatgctcat
     9181 gcgatgggga acggaccggg cgggctgacg gagtaccaga acgtcttcta taagcacgat
     9241 tgcattcagg gtcattatgt ctgggagtgg tgcgaccacg ggatccaggc acaggacgac
     9301 cacggcaatg tctggtataa attcggcggc gactacggcg actatcccaa caactataac
     9361 ttctgtcttg atggtttgat ctattccgat cagacgccgg gatcgggcct gaaagagtac
     9421 aaacaggtta tcgcgccggt aaaaatccac gcgctgactc gcggcgagtt gaaagtcgaa
     9481 aataaactgt ggtttaccac gcttgatgac tacaccctgc acgcagaggt gcgcgccgaa
     9541 ggtgaaacgc tcgcgacgca gcagattaaa ctgcgagacg ttgcgccgaa cagcgaagcc
     9601 cccttgcaga tcacgctgcc gcagctggac gcccacgaag cgttcctcaa cattacggtg
     9661 accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac ttatcagttc
     9721 ccgctgaagg aaaacaccgc gcagccagtg cctttcgcac caaataatgc gcgtccgctg
     9781 acgctggaag acgatcgttt gagctgcacc gttcgcggct acaacttcgc gatcaccttc
     9841 tcaaaaatga gtggcaaacc gacatcctgg caggtaaatg gcgagtcgct gctgacccgc
     9901 gagccaaaga tcaacttctt caagccgatg atcgacaacc acaagcagga gtacgaaggg
     9961 ttgtggcagc cgaatcattt gcagatcatg caggaacatc tgcgcgactt tgccgtagaa
    10021 cagagcgatg gtgaagtgtt gatcatcagc cgcacagtta ttgcaccgcc ggtgtttgac
    10081 ttcgggatgc gctgtaccta catctggcgc atcgctgccg atggccaggt taacgtggcg
    10141 ctttccggcg agcgttacgg cgactatccg cacatcattc cgtgcatcgg tttcaccatg
    10201 gggattaacg gcgaatacga tcaggtggcg tattacggtc gtggaccggg cgaaaactac
    10261 gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga tgccatgttc
    10321 gagaactatc ccttcccgca gaacaacggc aaccgtcagc atgtccgctg gacggcactg
    10381 actaaccgcc acgacaacgg tctgctggtg gttccgcagc gcccaattaa cttcagcgcc
    10441 tggcactata cccaggaaaa catccacgct gcccagcact gtaacgagct gcagcgcagt
    10501 gatgacatca ccctgaacct cgaccaccag ctacttggcc tcggctccaa ctcctggggc
    10561 agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg ctttacgttg
    10621 ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga gttcggcgca
    10681 gggttctttt ccacgaattt gcacagcgag aataagcaat gaggatcatc gataacttag
    10741 aacagttccg ccagatttac gcctctggca agaagtggca acgctgcgtt gaagcgattg
    10801 aaaatatcga caacattcag cctggcgtcg cccactccat cggtgattca ttgacctacc
    10861 gtgtggaaaa tgactccgcg accgatgcgc tatttaccgg gcatcgacgc tattttgaag
    10921 tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag acattacagg
    10981 tagtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc ggagaaaccg
    11041 tagaggtcca cgaagggcaa atcgttattt gcgatatcca tgaagcgtat cggtttatct
    11101 gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat cgaagatggt tatttccata
    11161 acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta cacattttcg
    11221 gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct tgctctcgct
    11281 gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg agcttggact
    11341 ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct tctgtctgat
    11401 catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg cgtgggtaaa
    11461 aagttcgctg ggcggacgtt gggcatttat tactgcctac acttactggt tcgtaaatct
    11521 gttctttttt acctcgctgt tgccgcgcgt tattgcttat gcttcgtatg ccttcctcgg
    11581 ctacgaatat attatgacgc cggttgccac caccgttatc agtatggtgc tgttcgcctt
    11641 ctccacctgg gtttccacca acggggcgaa aatgctgggg ccaattacct ccgtcacttc
    11701 aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg cactggttgg
    11761 cgacgtacag cctgccgatc cgatcaccgt tgacgcgatg atcccgaact tcaactgggc
    11821 gttcctcggc gttaccacct ggatctttat ggccgcaggt ggcgcggagt ccgtcgcggt
    11881 gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca tcctcgccgg
    11941 gatttttatc ggcgtactgt attccgtctc ctcggtgctg attaacgtct tcgtcagcag
    12001 caaagagttg aaatttaccg gcggatcggt gcaggtattc cacggcatgg cggcgtattt
    12061 tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta ccgcaatgtt
    12121 cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt ttcttctccg aaatcccgga
    12181 aggcatcttt ggtaagaaaa ccgtcgaact taacgaaaac ggcgttccgg cgcgcgcagc
    12241 atggatccag tttctgatcg tcatcccgct gatgattatc ccgatgctcg gttccaatac
    12301 cgtgcaggat ctgatgaata ccattattaa tatgaccgcc gcagcgtcca tgcttccgcc
    12361 gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc tgccacgcga
    12421 tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga ttgcgatatt
    12481 tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga ccatcatttt
    12541 ttataacgtc ggcggtattg ttatcttcct cggctttgcg tggtggaaat acagtaaata
    12601 tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca gcaatgttga
    12661 ttaagcagac taataaaaaa tgagcaggca ttttccctct tcctggatga ggagagggaa
    12721 aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac tttttcttta
    12781 gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta cttgctctgc
    12841 cgttttgcta cgccttttct gcggcaggag aagaagcacg tccggcagaa catgacgaca
    12901 caaaaacacc cgcaattacc tcgacatctt ctccttcatt tcgtttttac ggcgaattag
    12961 ggcttggtgg atatatggat ttagagggtg agaataaaca taaatacagc gacggcacct
    13021 atattgaagg tggcctggag atgaagtacg gctcctggtt cggcctgatt tacggcgaag
    13081 gctggaccgt gcaggccgac cacgacggta atgcctaggt gcctgaccat agctggggtg
    13141 gtttcgaagg tgggattaac cgtttctatg gcggttatcg taccaatgat ggcaccgaaa
    13201 tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg ggcgacttca
    13261 cacccgattt gggctacgtc attcccaata cccgcgacat tatgaccgcg ctgaaggtac
    13321 agaacttaag cggcaacttt cgttatagcg tcaccgccac acctgccgga catcatgacg
    13381 aaagcaaagc ctggctacat tttggcaagt acgatcgcta cgacgacaaa tacacctatc
    13441 cggcaatgga tgaacggtta catccagtat gaccttgccg aaggcatcac ctggatgaat
    13501 ggtctggaaa ttaccgacgg cacaggacaa ctctatctca cgggcctgtt tacccctaac
    13561 tttgccgctc gcgcctggca ccataccgga cgtgccgacg ggctggacgt accgggaagt
    13621 gaaagcggga tgatggtgag cgccatgtat gaagcgttaa agggcgttta tctctccacc
    13681 gcttacacct acgccaaaca tcgccctgac cacgctgacg atgaaaccac ctctttcatg
    13741 cagtttggta tctggtacga atatggcggc ggacgtttcg ccacggcttt tgatagccgc
    13801 ttctacatga aaaatgcctc taacgatccc agcgaccaaa tcttcctgat gcaatatttc
    13861 tactggtaat aaggactgta atttcatgaa aatcaaaact attttaacgc cagtaacctg
    13921 cgctctgctg ataagttttt ccgctcatgc cgctaacgcc gacaattata aaaacgtgat
    13981 taaccgtact ggtgcgccgc agtacatgaa ggattacgat tacgacgatc accagcgttt
    14041 taatccgttt ttcgatctcg gagcctggca tggtcatctg ttgccagacg gcccgaacac
    14101 catgggcggc tttccgggcg ttgcgctgct gacggaagaa tacatcaact ttatggccag
    14161 caatttcgac cgcctgactg tctggcaaga cggcaagaaa gtcaacttca cgctggaggc
    14221 atacagtatt cccggcgcgc tggtgcaaaa actgacagca aaagatgtgc aggtcgaaat
    14281 gactctgcgc ttcgccacgc cgcgcacgtc actactggaa actaaaatca tcagcaataa
    14341 accgctggat ctggtgtggg atggcgaact gctggaaaaa ctggaagcga aagaagggaa
    14401 accgctttcc gataaaacca ttgctggcga ataccctgac tatcagcgca aaatcagcgc
    14461 cacccgtgat ggcctgaaag tcacctttgg caaagtgcgc gccacctggg atctgctgac
    14521 ctccggcgaa tcggaatatc aggtgcataa atccctgccg gtgcagactg aaatcaacgg
    14581 taatcgcttt accagtaagg cgcatatcaa cggttcgacc acgctctaca ccacctattc
    14641 ccatctgctg accgctcagg aagttagcaa agagcaaatg cagatccgcg atattctggc
    14701 gcgtccggcg ttttatctca ccgcctcgca gcaacgctgg gaagaatacc tgaagaaagg
    14761 gttaaccaat ccggatgcga cgccggaaca gacgcgcgtc gcggtgaaag cgatcgaaac
    14821 gctcaacggt aactggcgct caccgggcgg tgcggtgaat tataacaccg tcacgccgtc
    14881 ggtgaccgga cgctggttct ccggcaatca gacctggccg tgggatacct ggaagcaggc
    14941 gtttgcgatg gcgcatttca atccggatat cgccaaagag aatatccgcg cggtcttctc
    15001 ctggcagatc cagcctggcg acagcgtgcg tccgcaggat gtgggctttg tccccgacct
    15061 gatagcgtgg aaccttagcc ccgagcgtgg tggcgatggc ggcaactgga acgaacgtaa
    15121 taccaaaccc agccttgccg cctggtcggt gatggaagta tacaacgtca cccaggataa
    15181 agcctggttg gcagagatgt acccgaaact ggtggcctat cacgactggt ggttacgtaa
    15241 ccgcgatcat aacggcaacg gggtgccgga atatggcgcg acccgcgaca aagcccacaa
    15301 caccgagagc ggcgagatgc tgtttacggt gaaaaaaggc gataaagaag agacgcagtc
    15361 tgggctgaac aactacgccc gcgtggtgga gaaaggccag tacgacagtc tggaaattcc
    15421 ggcacaggtc gctgcgtcgt gggaatcggg gcgtgatgat gccgccgtct ttggttttat
    15481 cgacaaagaa cagttggata aatatgtcgc taatggcggc aaacgtagcg actggacggt
    15541 gaaattcgcc gaaaaccgca gtcaggacgg aacgttgctg ggctactcgc tattgcagga
    15601 gtcggtggat caggccagct atatgtacag cgataaccat tatctggcgg agatggcaac
    15661 catcctcggt aagccagaag aggccaaacg ctatcgccag ttggcacagc agctcgcgga
    15721 ctacatcaac acctgtatgt tcgacccgac tacccagttc tactatgacg tacgtattga
    15781 agataaacca ctggcgaacg gctgcgcggg caaaccgatt gttgagcgcg gtaaagggcc
    15841 ggaaggctgg tcgccgctgt ttaacggtgc ggcaacgcag gccaatgccg acgcggtggt
    15901 gaaggtgatg ctcgatccta aagagttcaa cacctttgtt ccgctgggaa cggcggcgtt
    15961 aaccaatccg gcttttggcg ctgatatcta ctggcgcggg cgcgtatggg tggatcagtt
    16021 ctggtttggt ctgaaaggga tggagcgtta cggttatcgt gatgatgccc tgaagctggc
    16081 ggatacgttc ttcaggcacg ccaaaggatt aaccgccgat ggcccaattc aggagaatta
    16141 caacccgctg acaggcgcgc agcaaggcgc accaaatttc tcctggagtg ccgctcattt
    16201 gtatatgttg tataacgatt ttttccgtaa gcagtaattt gcaacggctg gcggatgcgg
    16261 cgtgaacgcc ttatccgccc tacatgtgca atcccccaag ccggataaaa cacggcaagc
    16321 gtcgcatccg gcgttaccac cgggcgtatt ctttttgaat cccatcacaa accccgcact
    16381 ccccttttcc cttttctcca tcgacggcta aattagaact catccgacca cataacaatt
    16441 attttacata ctggacaatt ttatgagcta cccgtcgctg ttcgccccgc tggatttagg
    16501 ttttaccacg ttaaaaaacc gcgtgttgat gggttcaatg cacaccgggc tggaggaata
    16561 cccggacggt gctgaacggc tggctgcgtt ttatgccgaa cgcgcccgtc acggcgtggc
    16621 gctgattgtc agcggcggca ttgcaccaga tttaataggc gttggcatgg aaggtggcgc
    16681 aatgctcaac gacgccagcc agatcccaca ccatcgcacc attaccgaag ccgtacatca
    16741 ggaaggcggc aaaatcgccc tgcaaatttt gcataccggg cgctacagct accaaccgca
    16801 tctggtcgca ccgtccgcat tgcaggcccc catcaaccgt ttcgttcctc acgaactcac
    16861 ccatgaagag atcctgcaac tgatcgacga tttcgcccgc tgcgcgcaac tggcgcggga
    16921 ggccggatac gatggcgtcg aagtgatggg ctctgaaggg tatttgatca acgaatttct
    16981 gacgctgcgc accaatcagc gtagtgacca gtgggacggc gattaccgca accggatgcg
    17041 atttgccgta gaagtagtgc gtacggtgcg cgaacgcgtc ggcaacgact tcattattat
    17101 ttaccggttg tcgatgctcg acctggtcga aggcggcggc acttttgccg aaacggtaga
    17161 actggcgcag gccattgaag cggcgggcgc gaccattatc aacaccggaa taggctggca
    17221 cgaagcgcgg attccgacca tcgccacgcc cgtgccgcgc ggcgcattta gctgggtcac
    17281 gcgcaaactg aaaggccacg tctcgctgcc gctggtaacc accaaccgga ttaacgatcc
    17341 gcaggttgcc gacgatattc tctcgcgcgg cgatgccgat atagtatcga tggcgcgacc
    17401 gtttctcgcg gatgcggaac tgctgtcaaa agcgcaatca gggcgagccg atgagatcaa
    17461 cacttgtatt ggctgcaacc aggcctgcct cgatcagatc ttcgttggca aagtcacctc
    17521 gtgcctggtg aatcctcgcg cctgccacga aaccaaaatg ccaatccttc ccgccgtgca
    17581 gaaaaaaaat ctggcggtgg tcggcgctgg acctgctggg ctggcgtttg ccattaacgc
    17641 ggcggcgcgt gggcatcagg taacattgtt tgatgcgcac agcgagatcg gcgggcagtt
    17701 taatatcgcc aaacagatcc ccggcaaaga ggagttttac gaaacgctgc gctactaccg
    17761 ccggatgatc gaagtgacgg gcgtgacgct gaaactcaat cacaccgtga cggcggatca
    17821 gttacaggcg ttcgatgaaa cgatcctcgc cagtgggatc gtgccgcgtg ttccgcctat
    17881 cgacgggatc gatcatccga aggtattgag ttatctcgat gtactgcgcg acaaagcgcc
    17941 ggtaggcaac aaggttgcca tcatcggttg tggcgggatt ggttttgata cggcgatgta
    18001 tttaagtcag ccgggcgaat ccaccagcca gaatatcgcc aggttctgta atgagtgggg
    18061 gatcgacagc agcctacaac aggctggtgg cttaagcccg cagggaatgc agatcccgcg
    18121 tagcccacgg caaattgtga tgctccagcg caaagccagc aaaccgggac aggggttagg
    18181 caaaaccacc ggctggatcc atcgcaccac cctgctctcg cggggtgtga aaatgatccc
    18241 aggcgtaagt tatcagaaga ttgacgatga cgggctgcat gtggtgatca acggcgaaac
    18301 gcaggtatta gcagtggaca atgtggtgat ctgcgcaggg caagagccaa accgcgcgct
    18361 ggcgcaaccg ctgattgata gcgggaaaac tgtgcattta attggcggct gcgatgtggc
    18421 tatggagctg gacgcacgac gggcaattgc ccagggaaca cggctggcgc tggagattta
    18481 aatcgttatt tgaagcgccg gatgcaacgc atccggcacg tcgcgttaat caataaacaa
    18541 ggcgggagaa atgccgaatc gcgttgccaa ttttttagcg tgttccagcg ttaatttcct
    18601 cttcccgctc aaaacgcgtg acaccataga tttactgcca atttccggca gatcggaaag
    18661 ggttaaacca tattgatcca taagggtacg aatcacggcg ataccgccag gcatggcttg
    18721 agccatggca ttaaattccg caaattcggg cgctgattct tcccacgcgg ttattttggc
    18781 acacaccaga tccagcaagg ggttttcagg atcgttgagc agcagatgat ctaccagttc
    18841 cagcgcctgg gtgtattgtt cctcgttctg aatacccgcc agaaaaggtg ccacagcagt
    18901 tagcttttct cctgcttgca agatgtcggc aatcgcaatc attttttccc cttagtacga
    18961 tgaacagcgg taaagaaatc gtattcttta tgcgtcataa cttcacgtat gtagcacttt
    19021 tgcgattcaa aaaagaccat tgctacaaca cgtaattcat tgcccccaac attgaaaaca
    19081 taatgcttat ccagatattt gaagttatcc agagatggga atactgcttt taatgactca
    19141 ggttttttga aatatccctt agcaatcgtg ttccccagag ccaccaactc cgttttatgt
    19201 tgcgggtatt tttccgcagc atctttcaat gctttttgag ttatcaggtg cattcttcat
    19261 cacgtccgtt gacaaattgg caatatgata acatccgttg acagattggc aacggatgaa
    19321 tttatctgtg tgacggaaga ataaacgtag aaaaacgcta aacaagagaa tgtagaggat
    19381 ggtgacaggg attttttgtt ttatggaagc gcgattccag aactgagcag acattgccgg
    19441 atgcgacgct tgccgcgtct tatccggcct gggggtaacg tgctgaacgt aggccggata
    19501 aggcgtttac gccgcatccg gcaggtaact cccaatctta ccgacgacgt cccagcttca
    19561 ccgctttcag caccacaaat ttattattcg tcgcaatagt ggtgcagttg ccgaaaattt
    19621 tcttcagttt atggaagtaa tccagatggc ggttggcaac gatatacagc tcgccgttga
    19681 ttttcaggca gcggcgggcg tggtggaaca tctcccaggc gacgttatcg gtcagcgcat
    19741 gttgttggtg aaacggaggg ttgcagagca cagcattaaa gcggaaaggc tccacgccgg
    19801 agagcgcgtt gttgatcata aactcgctgc gatccaacgc ctctggcatg ttggtttcaa
    19861 cgttcaaacg gctggaagca accgccatcg gcgattcatc gacaaacacc actttcgcct
    19921 gcgggttttt atcaagcagc gtcagaccaa taacgccatt accgcaaccg agatcgacaa
    19981 tctccccttc gagattctct ggcagatgtt gcataaagaa gcgcgcgccg atatcaagcc
    20041 cggtgcggga gaagacattc gcatggttat ggatagtcca gtcagtacct tccagcttcc
    20101 agctaacggt ctgcggcgca tcggccagcg gcggttcatt gaacgtgcaa ttaatcaggc
    20161 gcgctttttt ccatgccagc gtggtggtgg tcgggccgag cactttttcg aacagttcca
    20221 gcgtggaagt gtgaatgtca cgggctttgg caccggcaat aatacgtgta tccgacgtga
    20281 ccactttgcg cagcgcacgc agttgctgtt ccagcaatgc cagtgttttc ggcactttga
    20341 tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgagaaac ttcacgctcg
    20401 actcatcaat cccgtttagg cgtaaattct cgcgcgtcgc cagttcactg atgtatgagt
    20461 cgccaatgct gtacggctta tgttccgcca gcgcgcagct taacgcacca aaggcatcat
    20521 tcaggatcaa caccgggccg gggatttctg tgtcgtccaa ctgttgcagc aaatattcat
    20581 ctgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa cgttgtagtg
    20641 tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga taaaatttcg
    20701 acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt gggcggttat
    20761 gagcaatctt acttatcttc agggttatcc cgagctgctg ctttcgcagg tgcgaacact
    20821 gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgc tatccgggaa cacacgacta
    20881 cgccaccgat aaagctctct ggcaatatac tcaggatctg aaaaatcagt ttctgcgtaa
    20941 tgccccgccg atcaataaag tgatgtatga caataagatc catgtgctaa aaaacgcgct
    21001 cgggttacat accgctgttt ctcgtatgca gggcggcaag ctgaaagcaa aagcagagat
    21061 ccgcgtcgct accgtgtttc gcaacgcgcc ggaaccgttt ttgcgcatga tcgtggtgca
    21121 cgagctggcg cacctgaagg agaaagagca taacaaagcg ttctatcaat tgtgctgcca
    21181 tatggagctg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa cgcagttgtc
    21241 gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta tcttttcttt ctttagcgcc
    21301 taaaatcgac ctcccccctt tcgttaagga taacgaccat gctgcgcgca tttgcccgcc
    21361 ttcttctccg tatttgtttt tctcgccgga ctctaaagat tgcctgctta ctgttacttg
    21421 tggcaggagc gaccattttt atcgccgatc gcgttatggt caacgccagc aagcagctga
    21481 cctggggcga tgtcaacgcc gtcccggcgc gtaacgtggg tttattgctg ggggcgaggc
    21541 cgggtaatcg ctactttacc cgacgtattg ataccgcagc agcgttgtac cacgccggaa
    21601 aagtgaaatg gctactggtg agtggcgata acggacgtaa aaattacgat gaagcatcag
    21661 gtatgcagca ggctttgatc gctaaaggcg taccggcgaa agtgatcttt tgcgactatg
    21721 ccggattctc aacgctggat tcggtagtgc gtgccaaaaa ggtgtttggg gaaaaccata
    21781 tcaccattat ctcgcaagaa tttcataacc aacgcgccat ctggctggca aaacagtacg
    21841 gcatcgacgc tatcgggttt aacgcgccag acctcaatat gaagcatggt ttttatactc
    21901 aactgcggga gaaactcgct cgtgtcagcg cggtgattga cgcaaaaatc ctccatcgcc
    21961 agccgaaata tctcgggcca tcggtcatga ttgggccgtt tagtgagcat ggctgtccgg
    22021 cgaaagaata atgcgtatct gcgcacgtcg aagatgaaaa aggcgtgcta cattgacgac
    22081 agaatccctt tatggagtat ccacgcgtta tgatacgttt cgctgtgatt ggtacgaact
    22141 ggatcactcg ccagttcgtc gaggccgccc atgagagcgg taaatacaag ttaaccgccg
    22201 tatattcccg cagccttgag caggctcagc acttcgccaa tgatttttct gtcgagcatc
    22261 tgtttacgtc gctggaagcg atggcggaaa gcgatgccat tgacgcggtg tatattgcca
    22321 gcccgaattc cctgcacttt tcccagacac aacttttcct tagccataaa attcatgtga
    22381 tttgcgaaaa accgctggcg tcgaatctgg cggaagtgga tgccgccatt gcctgtgcgc
    22441 gcgaaaatca ggtggtgctg tttgaggcat ttaaaaccgc ctgcctgccg aactttcatt
    22501 tgttgcgcca ggcgctgccg aaagcaggca aactgcgtaa agtctttttc aactattgcc
    22561 agtattcctc gcggtatcaa cgttacctgg acggtgagaa tcccaacacc tttaatccgg
    22621 cattctctaa cggttcaatt atggatatcg gcttttactg cctggcgtcg gcggtggcgt
    22681 tatttggtga gccgaaaagc gtgcaggcaa cggcgagttt gctggcaagc ggcgttgacg
    22741 cccacggcgt ggtggtgatg gattacggtg atttcagcgt caccttgcag cactcaaagg
    22801 tcagtgattc tgtcctggga agcgagattc agggcgaagc gggatcgctg gtgattgaaa
    22861 aactgtctga atgccagaaa gtctgctttg tgccgcgtgg cagccagatg caggatctca
    22921 cccagccgca gcatattaat accatgctct acgaagcaga gctgttcgct accctggtgg
    22981 atgagcatct ggtggatcat ccagggctgg cggtcagtcg catcacagcc aaactgctga
    23041 ccgagatccg ccgccagact ggggtgattt ttccggcaga tagcgtaaaa ctatacttgc
    23101 caaagtaaaa cagtgtaaaa ggtatgtaac agaccattga ctgactgaat ggtctgtcat
    23161 actttgttac ctgcaaaggg gagtaacttc attgccggtc gatcgtcatt acgatgtgtg
    23221 aaaaaccaca tccggtcacc gggcaacccg aaaggaatac gcagacgtat tccttttttg
    23281 ttgtaagtga gaccttgccg gaaggcgagg tctatgcata aaaagcagcg gctgacgtct
    23341 tccgacgttg gccgtttttt tatgtgtaag gaacttctat gaatactgtc ggcacgccgt
    23401 tgctatgggg cggattcgct gttgttgtcg ccattatgct ggctatcgac ctgttgttgc
    23461 aggggcgtcg tggggcacat gccatgacca tgaaacaggc tgcggcctgg tcgctggtct
    23521 gggtgacgct gtcgttactg tttaacgccg ctttctggtg gtatctggtg caaaccgaag
    23581 gtcgcgccgt tgccgatcca caggccctgg cctttctcac gggttatctg attgagaaat
    23641 cgctggcggt cgataacgtc tttgtctggc tgatgttgtt cagctatttc tccgttccgg
    23701 cggcattaca acgtcgcgtg ctggtgtatg gcgtgctcgg ggcgattgtt ctgcgtacca
    23761 tcatgatctt cacaggcagc tggctgattt cgcagttcga ctggatcctg tatatcttcg
    23821 gtgccttcct gctgtttacc ggcgtgaaga tggcgctcgc ccatgaagat gaatcaggca
    23881 ttggcgacaa gccgctggtg cgctggctac gcggtcattt gcgcatgaca gacaccatcg
    23941 acaacgagca tttctttgtg cgtaagaatg gattgctgta cgccacaccg ctgatgctgg
    24001 tactgattct ggtggaattg agcgacgtga tttttgccgt ggatagcatt ccggctatct
    24061 tcgccgtgac cactgacccg ttcattgtgc tgacctcaaa cctgtttgca atcctcggcc
    24121 tgcgtgcgat gtatttcctg ctggcgggcg tagcagagcg tttctcgatg ctcaaatatg
    24181 gcctggcggt gattctggtg tttatcggta tcaagatgct gattgtcgac ttctaccata
    24241 ttccaatcgc cgtctcgctg ggcgtggtgt ttggcattct ggtgatgacg tttattatca
    24301 acgcctgggt gaattatcgg catgataagc agcggggaga atgattcgct gtatattaag
    24361 ccattcctag agataactca ttccagacaa aggtgagcta ttaattatgt aatcggctta
    24421 tagctcttta caaaaagacc gcacggatgt tggtggcgtc aaaatcgccg aagcgttccc
    24481 tggaggccga ggcgagggcg catcttacag ggacgttacc ttgcgcccga cccggtagcc
    24541 gcaagggata ggctgaggga accgcgcagc ggcgattttg ttcgccagag cctgggggtg
    24601 cagggggcgg cggcgattgg ccgccccctg cgcgctccct gcgccagtga ctttatatgg
    24661 cataacacgt taaaggagcg caaccgttct catattccaa atagttttgc ccatatgtta
    24721 tctaatcagc cttaatagcc agtccttccc tctgtttatg ttaaataaat gcaaccatta
    24781 agttgcaaaa catgacctct ctttaaaatc cagcattttt cgcttcccga agctgtaact
    24841 ttccttatac tcgaccttgc aaacactttg ttacatcctg aaagatgcgt cgacagaacg
    24901 caccagggat gtgcgacaac acaatgaaag gatcgaaaaa tgactacgca acgttcaccg
    24961 gggctattcc ggcgtctggc tcatggcagc ctggtaaaac aaatcctggt cggccttgtt
    25021 ctggggattc ttctggcatg gatctcaaaa cccgcggcgg aagctgttgg tctgttaggt
    25081 actttgttcg tcggcgcact gaaagctgtt gcccccatcc tggtgttgat gctggtaatg
    25141 gcatctattg ctaaccacca gcacgggcag aaaaccaata tccgtcctat tttgttcctc
    25201 tatctgctgg gcaccttctc tgcagctctg gccgcagtag tcttcagctt tgccttccct
    25261 tctaccctgc acttgtccag tagcgcgggt gatatttcgc cgccgtcagg cattgtcgaa
    25321 gtgatgcgcg gactggtaat gagcatggtt tccaacccca tcgacgccct gctgaaaggt
    25381 aactacatcg ggattctggt gtgggcgatc ggcctcggct tcgcactgcg tcacggtaac
    25441 gagaccacca aaaacctggt caatgatatg tcgaatgccg ttacctttat ggtgaaactg
    25501 gtcattcgct tcgcaccgat cggtattttt gggctggttt cttctaccct ggcaaccacc
    25561 ggtttctcca cactgtgggg ctacgcgcaa ctgctggtcg tgctggttgg ctgtatgtta
    25621 ctggtggcgc tggtggttaa cccattgctg gtgtggtgga aaattcgtcg taacccgttc
    25681 ccgctggtgc tgctgtgcct gcgcgaaagc ggtgtgtatg ccttcttcac ccgcagctct
    25741 gcagcgaaca ttccggtgaa tatggcgctg tgtgaaaagc tgaatctgga tcgcgatacc
    25801 tattcagttt ctattccgct gggagccacc atcaatatgg cgggcgcagc aatcaccatt
    25861 accgtgttga cgctggctgc ggttaatacg ctgggtattc cggtcgatct gcccacagcg
    25921 ctgctgttga gcgtagtggc ttctctgtgt gcctgtggcg catccggcgt ggcggggggt
    25981 tctttgctgc tgatcccact ggcctgtaat atgttcggta tttcgaacga tatcgccatg
    26041 caggtggttg ccgtcggctt tattatcggc gtattgcagg actcttgcga aaccgcgctg
    26101 aactcttcaa ctgacgtgct gttcactgcg gcagcttgcc aggcggaaga cgatcgtctg
    26161 gcaaatagcg ccctgcgtaa ttaattgttt aacccctttc gtctatggcg ggaggggttt
    26221 tctctacttt aaacggatca attccccttt tctgcatccg ccagaaacga atgatattca
    26281 ggccattcat aagcagaaaa ctaccctcaa tcatcgtgcc gcctatcgac cccgcccaga
    26341 agttgtgaat cacccagcaa cacgttgaaa accacatcac gcagcgcatg gtgagccctt
    26401 tacagcggaa cagcgcccag gtactgacaa tcgtaccgat aaccggcaat agttcgacag
    26461 gatgatggaa cttcgcgagg ccaattccgc cagtcagcac aataaaaatc gccattaccc
    26521 ataagctgcg cgtgcgtaag gtaatcaatg tacgaatggc attaaggatg gcactggcac
    26581 cagcgggata ggtgcccaga agaaaaaaat gtacgccaat aacggcgcta tagaccgaaa
    26641 gctgcttttt gaagcgacgt tcgtcacgat tgaaaaatgt tgtgatacca atcagaaagg
    26701 cgatgacacc cacgccctgg accagccaat acgcggtcat gataaatcct tagcaggtat
    26761 ggaaaagcaa acggcgcttc acattatgaa acgccgtttt ttattaacaa ctcatttcga
    26821 ctttatagcg ttacgccgct tttgaagatc gccagttcgc ggaagtcgtt acgctcgttg
    26881 caggtttgct taccgttggc aaactcaacg atggtgtcga taaattcttc cagcaactgc
    26941 ggcatcgctt taccgtggat cagctgaccc gcgtcaaagt cgatccagtg ttttttcttc
    27001 gccgccagtt cactgttggt ggcgattttc accgtcggca caaatccacc gtatggcgtg
    27061 ccgcgaccag tactgaacag caccatatgg cagcccgcac ccgccagggc gctggtcgct
    27121 acggcatcgt tacccggcgc acttaacaag ttcagccctg gcgttttcag acgctcgccg
    27181 taacgcagca cgtcaaccac gacactggaa cccgctttct gggtacagcc aagtgatttg
    27241 tcttccagcg tggtgatacc gcccgctttg ttccccggtg atgggttttc gtagatcggc
    27301 tgatcatggg caataaagta ctgtttgaag tcgttgacca tggtgaccag tttttcaaac
    27361 gttgcttcgt cgcggcaatg gtccatcagc aactgctctg cgccaaacat ctccggcact
    27421 tcggtcagta cggtagtacc gccgttagca atcacgtagt cagagaaacg ccccagcatc
    27481 gggttagcag taataccaga aagaccgtca gaaccaccgc actccagacc aaacttcagt
    27541 tcgctgagtt tgcctggctc gcgtttatcg ttgcgcatca cgttatacag ctgatgcaaa
    27601 tgctcgatcc cggcttcgat ctcatcatcc tgctgttggc agatcatgaa atgaacgcgt
    27661 tcaggatcga tatcgcccaa cgtttcacgg aatgcggcaa cctggttgtt ttcacagccc
    27721 agaccaatca ccagcactgc gcccgcgttc gggtggcgca ccatgttttg cagcatggtg
    27781 cgggtgttga tgtgatcgtc gcccagttgt gagcagccgt aggtgtggct gaagaggaac
    27841 acgccgtcgg taccttcggc gttgttggtc tctttcagga aacggttctg gatttgccgc
    27901 gcgatgccgt tgacacagcc cacggttggc aggatccaca gctcgttacg caccccgaca
    27961 tcgccgttag cgcgacgata gatctgcact tcaggatctg ccgcttgcgc aggcagatcc
    28021 tgaaaatcag gttgatagcg atactgatcc agatcgctca gattcgtgcg cgtattgtgg
    28081 gcgtggacgt gttcccccgc cgcaatatcc gccaatgcat aaccaatcgg caggccatat
    28141 ttgatgacgt tggccccttt tgcgatatcc gttaacgcaa atttatgtcc acgagcaaca
    28201 tcctggcgca gcgtaacagt ctggttatcg acactgactt ctgtgccttc agccaaatct
    28261 gctaaagcga ccgcgacgtt atccagcgca tggatcttga tgtattgcat atcaacccca
    28321 gaccttagtt cagttcaatg gcgaagtagt cacgcgcatt gttaaagcaa atatttttca
    28381 ccatctcgcc cagcaggttg atgtccgccg gtgcttcgcc cgcttccacc cagcgaccga
    28441 tcatctggca cagaatgcgg cggaagtatt cgtgacgggt gtatgacagg aagctacggc
    28501 tgtcagtcag cataccgaca aaacggctca gcagaccgag ctgcgccagt tgggtcatct
    28561 gacgttccat accgtctttc tgatcgttaa accaccagcc ggaaccgaac tgcattttgc
    28621 ccggcatacc ttcgccctgg aagttaccga tcatggtgcc cagcacttcg ttatcgcgcg
    28681 ggttcaggca gtagagaatg gttttcggca gcaggttttc ttcgttctgc ttgctcagca
    28741 gcttagacaa ctcttccgcc atcggacggt cgttgatgga gtcaaagcct acatccggcc
    28801 ccagcagttt gaactgacgc aggttgttat tacgcagcgc gccaatgtgg tactgctgta
    28861 cccagccgcg acgtgcatat tcggcaccaa ggaacaccag taccgcagtt ttgaactgtg
    28921 ccacttcgtg ctcgctcagg gtttcgccag ccagacggcg cgccagaatg ctgtcgagtt
    28981 ccgcttcgtt cgcttcagca aacatcacca cgtccagcgc atggtcagac actttacagc
    29041 cgtgagcggc gaagtgatcc agacgtttgg tcaaggcagt ttgcaggtca gcaaagcggc
    29101 gaatgtcggt atcggaaact tcgcccagct tcgccatgta gtcgttaaag gtcgcctgtt
    29161 cgatattgaa ggctttgtcc ggacgccagc tcggcagcac tttgatggtg aaagagccgt
    29221 ctttggcgat ctctgcgtga tgctccagag aatcgatcgg gtcatcggtg gtgccgacca
    29281 ttttcacgtt catctgctgc atgatgccgc gcgcggagaa gttatcctgc gccagcagtt
    29341 cgttacattc gttccagatt tcatcggcag ttgacggaga aagcaatttg ccagtgatac
    29401 cgaacggacg acgcagttcg aggtgcgtcc agtggtataa cgggttgccg atagtgtgcg
    29461 gaacagtcgc cgcccaggcg tcaaattttt cacggtcaga cgcatcacca gtacacagac
    29521 gctcagccac accgttggta cgcatagcgc gccatttgta gtgatcgccc ttcaaccaga
    29581 tgtcatacag gtttttaaaa cgatagtctt ccgcaatctg ctgcggcggc aaatggcaat
    29641 ggtaatcgaa aatcggctgg tcttttgcgt agtcgtgata cagacggcgg gcaaattcgg
    29701 tatctaacag gaaatcttca gtcataaacg gagtcatttt cgtcttcctc tcaacgagtg
    29761 agctagcttg cttatggtgc gatgctgaca aagttatcac accaatttcc agagtccgaa
    29821 gatattttcg tgagttagat caataaacgt agttaaaaaa attactctca aagtggtaaa
    29881 tctcgctgca ggccgcgcca gtactggcct cgctgtcgtc aggtaatgtc cctacaaata
    29941 ttcgcacatt tgtgatggct ctcacctttt aaggttgtat gacaagttat ctttctgccg
    30001 tcgcaaatca taagtcgacg gaatgcaaat tgccgattca ttcatttgtt agatgaatcg
    30061 ggttaaccgg tacggaagcc gaattagcac gaaactttca tggcaacgtt cggggcgtgc
    30121 cggttttttt tcggttaccc ggtcgtaact aacatcttca gcctctggcg ggatgatggc
    30181 cgcgcttcct gcggatataa caaaacgatg aggttttaca tgcgtaaaat taaagggtta
    30241 cgttggtata tgatcgcact ggtgacgctc ggcaccgtgc ttggttacct gacgcgtaac
    30301 actgtggcgg cagctgcgcc aactctgatg gaagagttaa acatctccac ccaacagtat
    30361 tcctatatca tcgcagccta ttctgctgct tatacggtca tgcaaccggt agcaggttat
    30421 gtactggatg tgctgggtac gaaaatcggt tatgcaatgt ttgctgtgct gtgggccgtg
    30481 ttctgtggtg caaccgcgct ggcaggtagc tggggtggcc tggctgttgc tcgtggtgcg
    30541 gtcggtgccg cggaagcagc gatgatcccg gcgggtctga aagccagctc cgaatggttc
    30601 ccggcgaaag agcgttccat cgcagtaggc tactttaacg taggttcttc gattggtgcg
    30661 atgattgcgc cgccgctggt ggtatgggca atcgtgatgc acagctggca gatggcattt
    30721 atcatctccg gtgcattgag ctttatctgg gcgatggcat ggctgatttt ctataaacat
    30781 ccgcgcgacc agaagcatct gaccgatgaa gaacgcgact atattattaa tggtcaggaa
    30841 gcccagcacc aggtgagcac ggcgaagaaa atgtccgttg gtcagatcct gcgtaaccgt
    30901 cagttctggg gtatcgcact gccgcgtttc ctagcagaac cggcctgggg tacttttaac
    30961 gcgtggatcc cgctgttcat gtttaaagtt tacggcttta acctgaaaga gatcgcgatg
    31021 ttcgcctgga tgccgatgct gtttgctgac ctcggttgta tcctcggtgg ttacctgccg
    31081 ccgctgttcc agcgttggtt tggtgtgaac ctgatcgttt cccgtaagat ggtcgtaacg
    31141 ctgggtgctg tgctgatgat tggcccgggt atgatcggtc tgttcaccaa cccgtatgtc
    31201 gcaattatgc tgctgtgtat cggtggtttt gcccaccagg cactgtctgg cgcgctgatt
    31261 acgctctctt ctgacgtgtt cggtcgtaac gaagtggcga cggcgaacgg tttaaccggg
    31321 atgtccgcat ggctggcaag tacgctgttt gcgctggttg tcggtgcact ggctgacacc
    31381 atcggcttca gcccgctgtt cgcagttctg gcagtatttg acctgctggg tgcgctggtt
    31441 atctggaccg tattgcagaa caaaccggcg attgaggtgg cgcaggaaac acataacgat
    31501 cccgcaccgc aacattaacg tttaacgtaa gttataacga cgaagccgcc ttatcaggcg
    31561 gcttcgtcgt ttatatagaa aaaagcgttt gtccggtttg cagggttaat tgatggttgg
    31621 caaggaaggc gtaaagatta ctgacaattt gcccatgtag tacccggcct gtttatccat
    31681 cacgctgaat ttatcgactg ccagagtcaa gggtgtcggc acacacatca cggcatagcg
    31741 aataccgggc agtaccaccg gacgcacacg tttcaggtta atgctgttcc agaccatatt
    31801 ttcattattg cgcacatcaa tttgcccacc ggtttccggg ttgtacattt tgacccggta
    31861 atcaatgcgt tctccctcgt cggtggtggt ccctccctga cgatagatag agaagtaacc
    31921 gtctctgcgc cctgcagctg gtcgcccttc atctttgagc atgatttcat aactgatggc
    31981 attaccgtta aaaccatcgt acaggcacat gtccaacatg gtcagatctt gcgcgtagtt
    32041 gctgccgtta acgcttcctg ttggatgcaa gtctaactgc acccttggcg tggcgtgggc
    32101 gaactccggg aagaacacgt caatatggtt ggggtcgagc gtattcaggg taatatttgc
    32161 gaaatagtcc tgggccgggc tgttcgaatg taatttaact ttccccttcc agacgccacc
    32221 aatcggcaaa ttcttcatct ctgtctgctc aaggtagagc gtaagaaact ttgtatcgta
    32281 tatataataa tgttgaccat cttctgtaca tgaagatcta ttagaatcct taagattcgt
    32341 ctgaagccct ttgcccccct tgcatcctag cggcgcttca tcgctactta caatggctgt
    32401 tttaaatcct ttgagtttta ccggccaacg catcccactg cgttgttcag ttaaatagat
    32461 ttgtactgtc gatatatttc cattagctgt acaggtttgt tggaggtcag gatacgaaca
    32521 ttcataccac atatatccat tatttcctgc cgacgcttca tccttccaga agtaataatc
    32581 cgctctacca gatgccaaat catacgtaat ttcactggtc acgtcaggag ctgcctgaac
    32641 gccagtgagc gtgccaaaca agcccaatat cgccgcaatc aatcgatttc tcatggtgca
    32701 acctcccttt gcgccgtctg aacgtttcca cgtaatttag gctgtttcaa ctgtgcctga
    32761 acctgaatcg actccggtaa ttgtgaataa gtcaccacac tacaagtaac atctccgctg
    32821 taacgaatgc tggcgcgttt ctttacaaca ctcagtggac acttaaggaa ttgctgcccg
    32881 gaaagcagat agagctcttt ttcgctgccg ttaatttcaa gtgtaaatcc gccatcttca
    32941 gctaccagta acatggcgct gttcagcccc acaatcggga attttttcac cgctgccggc
    33001 agtaataaac gaccgatata gttgtagtta aagctggcag acacttcgcg atagcgcagc
    33061 ttgccaggca acaacattaa ttcacgattc cctgcaccgt taatgatgtt tgttgtccct
    33121 tgctgattgc cctggctact gtcgttaacc tcaacaaaac cagactgaaa tccaggataa
    33181 gggaacaaca cactgtcact ttgaccaagc tgcaaagttc ggctgcctgc cacccgcgct
    33241 tcgagttgca gatcctgact ctcttcatcc tgttcagaga agcctttcac atccaccagt
    33301 accgccccca gcaaatcgtc tgtaccactg gctcccaaat taacgccata gcgactgaca
    33361 gccagcgtag agctataagt gcccgtaaag gccgaaagat gatcatgatt cttacggtca
    33421 taactatcag atacggtagc gtagacattt ccgtattgcc catcccaacg cccgttaacc
    33481 gcgctgttta tggtatcggt gttcaggcct ccgacggtta cgccaagttc tttatggctg
    33541 aatgaatcgt tataaaaggt atgcgataac tgccatgacg tttgatcgcc atcctgttcg
    33601 ctataacgat aatccgtaga cacattggtt gaatggctgt tgttattgct atccatcgtt
    33661 ggcgtgtcag ataacgaaaa cgataagtac aagccgttgt cgtcgttatc gccaccgttg
    33721 tcattacgat ggaaaacacc gactgacgta ctcaaaatcc agtcacgcat cacaatagcg
    33781 cgggaagcgc taagttgtac agtttcagag atactttgcg ctgttgtctg gcgaagatag
    33841 ttattttcaa agctctgctg tttatcccaa cgcaaactat catcagtgtt attttgtgtg
    33901 caggtataac ccagcatcat attccagccg acaaaattca gtgaaactgt cgcggaaata
    33961 ctttcataac agcttaaggc gttatagctc tctctgctgt cggttgtaca agcatcgccg
    34021 tcagagttgg tccgataaaa gcccaacgtc ggccatcccg gattactcca gttggcctgt
    34081 tgcatatcac ctttgccgcc gtcatcgtta cggaacacgc tggcgctgat tgcaagattc
    34141 cccaccccgc ctaaatctgc cgtccagtta ttacctaact cgaaagcact cacatcatcg
    34201 gcattcgcca gccctgcgta gagctcatat tgcggatgta atggcaggcg tactcctagc
    34261 tgataagctg aactttcatc atcagaaacc tgtgatgtag ttttacctgc ctgtaagaac
    34321 cattgcgcat ttccgtcagt cagaccgccg gttttggtaa acggcacaag ctcggtgcag
    34381 gtgagttggt tattttcgta gacttttaat gccacgctgt agctgcctgg cggaaaggaa
    34441 ctggtatcaa taaattgcga accactattg agataaaacg atcccaaaag ttgctcatta
    34501 cgataagcgt caacacgaga attgcgcgaa agcagaacca ttactggggt tccttgctgg
    34561 ctttgcgcct ggtttaaata gctcagtgtc gacccgatac gcatcccatc gattgcaccg
    34621 agtggcagaa agttaaaggt aaagttcccg ccttgtgcat taaatagtgt gcggttgtcc
    34681 atgcgacccg cctgcacata ataacgacgt aaaaaatcat aacgataata gagatcgctg
    34741 acgtcagtat tactgtcact gacatcatct gcatcataac cgttgaaatt ccagtgtgca
    34801 ccaatatagc tattttctgt tattcccagc gcaccgtttc cctgaataga caacgattgg
    34861 taatcatcct gtgccagaac attgatgtct tgctgatgta taaaagcgtt aacagtattt
    34921 ttgtctggat ttaaatataa tgacttcgca tcgaaagctg aattccattg cggattaata
    34981 aacaacgttg ccgaactttc ttcatcatta tagattattt ccagcgtatc ggtattcaga
    35041 aaaccacacc ctgagtcagt acgtacataa ccgcaagcca attcaccgtg gcgagctagc
    35101 ggctgcgata atttttcttt cactatatgc gcgattttct gatcgtctac atccagttcc
    35161 agcttgttat atagacttgc tggatcaaga aatgttacgg tatcaagatt aaccattgca
    35221 taatatttac cgagactttt ccctgaaaaa taaacctcga tatactcctg ctgtccctga
    35281 gcaataacct caaaccctgc gggaattttg gttaatgtcg cctctgccgg gctgagactc
    35341 gccaggatca aaagagccag taatttttta tccatggcaa tcctgaaatc cataacaaat
    35401 actaaaacag ttaaaataag ggagggctac ccctcccttt gttacgtgtt aattctgtta
    35461 tccggattaa atatcctgag acagataaat gcttactaca ccacggtaga tgcctgtttc
    35521 cagaacacct ttggtggttt gagcaaacat caggttttta gccagagaac cgttagttaa
    35581 cgcatcggaa gcaaagattt tggttgcggt aaaagtggtt gccgcatcag ctttaatttc
    35641 ttcaccaccc caggttactg tcagcggaac aatcttgcta gcatccaggc tttgaaccag
    35701 ttgcgctggg gacactaact gcatttttac atctttggta acgtcgttag accagatttt
    35761 ggtcatcaac tggtaagatt gcagaccctg ccccggcaga tattgcattt ctactgcttt
    35821 cggcaacgcg gtgttatcgg tctgtgtcat atccagagcg gcatcaacgt tggccgttac
    35881 agtaatatcc ttttgtactg ccaatgcgga gcctgcaaca gaaaacatcg ctgcgaccag
    35941 aagagatttt gcaaatacct ttttcattac tattccttcg tttattacaa tgataattaa
    36001 tggaccgcca gttcaaactc gccattatct ttggtgatcc agttattata tttgacaacg
    36061 attttgtcgt agccagatat gctttcaaga tttctttcct gaccagggaa gatgttttta
    36121 tttacggtct gtatcttaca gttttctttg ctattatttt tacaataagc gatcgttaaa
    36181 agctttaagc gttgattccc tgcattaatt aatttttggg cagaatttac ttctaactta
    36241 gctataggtt gctgaggtgg aacactaaca acgattcccc aaattaagtt aactgttaac
    36301 tgcatagaga gatctttttt atctgtggca acgtcatcaa tactgggaac cgcttcaaat
    36361 ttaacgcgat aatttttctc tttttgctct ggttccatcg caacaaaacg tacggttttg
    36421 ctactaccgg ctggtaaagc gaatttaggt ggcataacaa ccagatgatt tgcatcaccg
    36481 gatttaatct caacttcatt ttcctgaggc gtcgatggat tatcgatacg gaatactgtc
    36541 gccttgatgt actgaacttc attactttta gatattacgc gaacgttgcc ttcaccctga
    36601 ctattaatgg aaacaggcat aggataaaca gtcatatttg actgtgccaa aaaaggagcg
    36661 aacacgaata aaagcggcgt aagccgtaat aagagattaa ttttcgccac catataattt
    36721 tgatcttaca atatcgtttc aaaaaagttg gtgtattaga gatgtagatt tattgtaagt
    36781 caatataaaa aagcattttt agttaacatt aaatttaaag gctgggaatt aacttactga
    36841 aatttagtag ctttcacaat gacgatactt attttttagg aatttataaa tattcacaat
    36901 attaatttaa tacattaatt caatcatcaa atttatatat gaccaatggc atacccatga
    36961 ataaagtggt atgataactt cgtccgcatt ccactccggg agacgcatat ggaaatcact
    37021 gaaccacgcc gtttgtatca acaacttgct gctgacctga aagagcgcat tgaacagggc
    37081 gtctatctgg tgggtgataa actgcctgcc gaacgcttta ttgccgatga aaagaacgtc
    37141 agccgaacgg tagttcgtga agccatcatc atgctggaag ttgaaggcta tgtggaggtg
    37201 cgtaaaggtt caggtattca tgtggtttcc aaccagccgc gtcatcagca ggcggctgac
    37261 aataatatgg aattcgccaa ttacggtccg tttgagttgc ttcaggctcg ccagctcatc
    37321 gaaagtaata ttgccgagtt cgcggcaact caggtaacga aacaagacat catgaaactg
    37381 atggccattc aggaacaggc gcgcggcgaa caatgcttcc gtgattccga gtgggattta
    37441 cggttccaca ttcaggtagc cctggcgacg cagaactccg ccctggcggc catcgttgaa
    37501 aaaatgtgga cccagcgtag ccataacccg tactggaaaa aactacacga acacattgat
    37561 tcccgtaccg ttgataactg gtgtgatgac cacgatcaaa ttctcaaggc gttaattcgc
    37621 aaagatcctc atgctgctaa gctggcaatg tggcagcatc tggaaaacac caaaatcatg
    37681 ttatttaacg aaaccagcga cgatttcgag ttcaatgccg accgctatct gttcgccgaa
    37741 aacccggtag tgcatctcga tacggccacc agcggcagca aatgaaattc ctgtccgaca
    37801 ggcgctgcaa tgcgcctgtt tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt
    37861 gtaaatcctc tcgccaccct cccctgcaat cagcaaaatc agactccacg gacatgtaat
    37921 tttgataacg aacaacgttg acctttgtta caattagatt caatttgaat ttatgctttt
    37981 gaatgctttc ttatctcacg ttttaacagg gaatagttca ggctgtgttg atgtatcaaa
    38041 cccgcagaac ataccaaaac agcaataaca ttgcggtagt gcatctttta aaaccagcgt
    38101 ggcgttaacc gattcaccag gaataatgaa tggaactttt gacccaattg ctgcaagccc
    38161 tgtgggcgca ggattttgaa accctggcca atccatcgat gattggcatg ttgtattttg
    38221 tcttgtttgt aattttgttc cttgaaaatg gcttgcttcc ggcggccttt ttaccgggcg
    38281 acagtttact ggtattggtc ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa
    38341 cgattctgct gctgaccgtt gccgccagcc tcggttgctg ggtcagctat attcaggggc
    38401 gatggctggg caatacccgc accgtacaaa actggctatc tcatttaccc gcgcattatc
    38461 atcaacgcgc acaccatctt tttcataaac acggtttatc ggcgctgtta attggtcgct
    38521 ttattgcatt tgtcagaaca ctgctgccga cgattgccgg gttatcaggg ctgaataacg
    38581 cgcgctttca gttcttcaac tggatgagcg gtctgctgtg ggtattgatc ctgacaactc
    38641 tgggttacat gctcggcaaa acgccggtat ttttaaagta cgaggaccag ctgatgtcat
    38701 gcctgatgct gctcccggtg gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt
    38761 tatggaaaaa gaaatatgga aatcgggggt aagggatgca aatacctcgc atgtcgcttc
    38821 gccagctagc ctggtccggc actgttttac ttctggtcgg cacgctgttg ctggcctggt
    38881 ccgcggttcg ccagcaagag tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga
    38941 tgccagacgg tttttcgatc tggcatcacc ttgacgctca tggcattcct ttcaaaagta
    39001 tcacccccaa aaacgacact ctgttaatta catttgactc cagcgaccag agcgccgccg
    39061 caaaagcggt cctcgacaga acattgcccc atggctacat cattgcgcag caggacaata
    39121 acagtcaggc tatgcagtgg ctgacccggt tacgggataa ctctcatcgc ttcggataac
    39181 ttcctgttat ccgaaacata tcactcactt tggtgatttc accgtaactg tctatgatta
    39241 atgaggcggt ggttaacact accgcttcgt tgtctggaca atcgttcctt tgtaataggt
    39301 ccgaatcaca atggaaggtt caagaatgaa ataccgcatc gctttagctg tttctctctt
    39361 tgctcttagt gccggtagtt atgccactac cctgtgtcag gaaaaggagc aaaatatcct
    39421 taaggagatc agctatgccg aaaaacacca aaaccagaat cgtattgacg gtctgaataa
    39481 agccctgagt gaagtccggg ccaactgttc agatagccag ctgcgtgccg atcatcacaa
    39541 gaaaatcgca aagcagaaag atgaggtggc ggaacgccag caagatttag ccgaggcgaa
    39601 gcaaaaaggc gatgccgata gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc
    39661 tggaagcgcg cgactactaa ctcacaatag tcactactta ctcacctgga gaaaactatg
    39721 tcgaaagaac acactacgga acatctgcgt gctgagttga aatccctttc cgatacgctg
    39781 gaagaggtgc ttagctcatc tggcgagaag tcgaaagaag agttgagtaa gattcgtagc
    39841 aaagcggagc aggcactgaa acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc
    39901 gccaaacaaa cccgtgtcgc ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg
    39961 accggcgtgg gaattggcgc tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt
    40021 taattatggc ggacactcat cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc
    40081 agcgaattgt ttccatcatg gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg
    40141 agctggaaga ggaaaaagct aatctctttc aacttttact gatgctgggc ctgacgatgc
    40201 ttttcgctgc atttggtctt atgagcctga tggtgctaat tatttgggcg gttgatccgc
    40261 aatatcgcct gaatgcgatg attgccacca ccgtggtgtt gctgctactg gcactgattg
    40321 gcggtatctg gacgttacgt aaatcgcgta aatccacctt gctgcgccat acacgccatg
    40381 agctggcaaa cgatcggcaa ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt
    40441 gaacgacgta aggcgcaact gcttagccag atccagcaac aacggctgga tctttccgcc
    40501 agtcgtcgtg aatggctgga ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta
    40561 agtctgcgct cctgggcgct ggttggcagt agcgtgatgg cgatctggac gattcgccat
    40621 cctaatatgc tggtccgctg ggccagacgc ggttttggcg tatggagcgc ctggcgtctg
    40681 gttaaaacga ccctcaagca gcaacagctt cgcggttaac ttccctctgg ccggagcaat
    40741 tccggcctta tccctcaaat tttttgaata tttttgacag ttttccttgc taacaatcat
    40801 cattcaccac gtttatgatt ctctccatcg acagcaacga cgctaatacc gcgcccttgc
    40861 acaaaaaaac aatcagcagc ctgagtggcg cagtggagag tatgatgaaa aaattagaag
    40921 atgttggtgt actggtagcg cacattttaa tgccgattct gtttattacc gcaggctggg
    40981 gaaaaatcac cgcttactct ggtacccaac aatatatgga agcaatgggt gtcccgggtt
    41041 ttatgctgcc gctggtgatt ctgcttgagt tcggtggtgg tctggcaatc ctattcggtt
    41101 tcctgactcg caccacaacc ctgtttactg cgggctttac gctgctgacg gcatttttat
    41161 ttcacagcaa ctttgctgaa ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt
    41221 ctggcggatt cctgctgctg gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc
    41281 tgaataaaaa gtggtaagca cgcgctatac ttaacgataa aaaacgagga ggaagctcct
    41341 cgtttttgct attggaggag agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca
    41401 cctggtacga taccaaatct accggcggta aatttcaacg ttcagcttcc gcatttcgta
    41461 actggctcac tgccgatggc gcacctggcc ccactggcac aggcggtttt atcgcagaga
    41521 aagatcgtta tcatctctat gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca
    41581 tgcgcaaact caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc
    41641 tggaaaacgg ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc
    41701 aacatgaatt tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta
    41761 ctgttcccgt gctgtgggac aaaaagaacc acaccatcgt cagcaacgaa tcagcagaaa
    41821 tcatccgcat gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc
    41881 caccagccct gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca
    41941 acggcgtgta taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga
    42001 aagtgtttga atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg
    42061 gcaaccagct aaccgaagcc gatattcgcc tgtggactac gctggtgcgt tttgatccag
    42121 tgtatgtgac ccacttcaag tgtgataagc accgcatcag cgattacctg aatctgtatg
    42181 gcttcctgcg cgatatctac cagatgccgg ggatcgccga aactgtcaat ttcgatcaca
    42241 tccgtaatca ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg
    42301 gtccgtggca ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg
    42361 caccttacga ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg
    42421 tgaacgcctt atcaaaccta caaagccttt atattcaata tgttacaatg accgcgtagg
    42481 ccagataagc gtagcgcatc tggcgatttt gcgcttgcca tcagtcttag cgcgccttca
    42541 cggcacttta ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta
    42601 atttgctgct ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg
    42661 aaagtactaa aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg
    42721 tttattctgg tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc
    42781 tggcaacgcg ctggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta
    42841 ccgtggtggg cggttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca
    42901 ctgctattct taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg
    42961 ggtacgccag gcgaaaaccg ctttggacca gatccaaaac tggaacaaga ataatgatta
    43021 agcaccttgt aaaaggtgct tcaaattgtt gacaaagtgc gctttgttta tgccggatgc
    43081 ggcgtaaacg ccttatccag cctacaaatt gccaaaattc aatatattgc agtgactgcg
    43141 taggcctgat aagcgtagcg catcaggcag ttttgcgttt gtcagcagtc agaagcacct
    43201 tgtgaaaggt gcttaataga ttattttcca gcgaacaact ttggaatttc ccgcagacac
    43261 caggatttcg cttcccccat actgtcacga cgccaggcca taataatatc gatctcgctg
    43321 gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa tatctttttc gaccatcgga
    43381 tacggcatcg tcgccacgcc aagccccgcc agtaatgcct gacgtttatc ttcaatcgtg
    43441 ctcaccgtta agcgcggctg tttgtccagc agctgtacgg tcaacaccgg gcgctcacga
    43501 gcggtatccg ctaccgcaat tccacgatat ttcacacgcg tcacttcaga taacggttcc
    43561 ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa cgtatagagc
    43621 ttgcgcgagt tgatctccga cgaggaacga aaatgcatat ccggcgcgat aacgatatcc
    43681 gcccgcccct gctccagccg ttcccacgcc cccgccagca cttctgtgat gattgccagt
    43741 tgggtattgg cttttgccgc cagtttgtcg attaacggga aaaaggcagg tgtcggtacc
    43801 agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc gcgcgagagc ttccgcatca
    43861 gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc gctccagcaa catcctcccg
    43921 acattggtga atttggtgcg atggcccgag cggtcaaaca gcaccacatc cagctcttct
    43981 tccagttttt gcatgatgta gctaagtgcg gaaggcacgc gtcccagctc atccgctgcc
    44041 gccgcaaaac tgccccggcg atcgatcgca tccataaccc gtagtgcttc cagcgttaat
    44101 gccctttctt tggccatttc gttctcattc aggaaatttg aacataccgg gcagaatatc
    44161 tggctaacaa tgtagcgtcc agccccttac cataaaagga agtaaagaga ggtcaagaat
    44221 tatgattact acccgaactg ccaggcagtg tggacaagca gactacggat ggttgcaggc
    44281 ccggtatact ttttcctttg gacactactt cgacccgaaa ttgttaggct atgcctccct
    44341 gcgtgtgctt aaccaggaag tgctggcccc aggtgccgcc tttcagccgc gaacctatcc
    44401 caaagtcgat attttaaatg tgattctgga tggagaagca gagtatcgcg acagcgaagg
    44461 caatcatgtt caggccagcg ccggagaggc gttgctgctc tctacccagc cgggtgtcag
    44521 ctatagcgaa cacaatctca gcaaagacaa accgttaacg cgaatgcagc tttggctgga
    44581 tacctgcccg cagcgagaga atccgctgat tcaaaagctg gcgcttaata tggacaagca
    44641 gcaattaatc gcctcgccag agggtgcgat gggaagcctg caattacgcc aacaagtgtg
    44701 gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc atgggccacg
    44761 cgcgtatttg caatcgattc acgggaaatt tcatgcgctt acgcatcatg aagagaaagc
    44821 ggcgctaacc tgcggtgatg gggcgtttat tcgtgacgag gctaacatta cgctggttgc
    44881 cgattcccca ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt tacagggaga
    44941 tgatgatgag taaaaaatcg accaaaaagc gccagctagc gaagcccgtg gtggcgaaag
    45001 aacctgctcg caccgccaaa aattttggct atgaagagat gttgagcgag ctggaagcta
    45061 tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc taccgcgtaa agacaggttg
    45121 ccggatgcgg cgtaaacgcc ttatccggcc tacaaaacac aacaacatca acaaattaaa
    45181 caccccgtag gccggataag atgcgccagc atcgcatccg gcatcaggta caaaacacaa
    45241 caacatcaac aagttgagca ccccgtaggc cggataaggt gcgccagcat cgcatccggc
    45301 acaatactca aaacctggcg tttatctggc cttgctcgcc ataatctcga taatctgccg
    45361 atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac
    45421 atcatgcgcc acgatccctt cattgccggt cacggcggta tcatccagcg ccattaacac
    45481 cgctttccac gcagccgaag cactggtcga aaccttcgca ccatcgcaaa tcatgccgct
    45541 gacatcgccg atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag
    45601 ccatgccatc ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa
    45661 acgcggtaac tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc
    45721 cagtcgttca tcatccgctc cgaagtgttc tgctacaacc accacaggca ttgttgcggt
    45781 gatcccctgg ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg
    45841 cgcatcggat gccgcgctgg tacgaatcac aatggatgaa gagagatctt tagccagcaa
    45901 accgcgctcg cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa
    45961 accttcctgc gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc
    46021 aaatgggact tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt
    46081 cagcggcgac tcttgctcgc cctctgccac acacgcctgc tgggtaaaca ccacaccatt
    46141 gtgcgtctca atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt
    46201 ctcaccgttc cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt
    46261 aacagagact ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc
    46321 gtctttcagc acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc
    46381 aatcggcagc cccaccattc ccgtgccggg aacggtgatg cccagaccgt tcttcatcag
    46441 atttggcgaa acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac
    46501 cgccgccgcc agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc
    46561 ctgaacggcg aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa
    46621 aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa
    46681 accagtgacg ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt
    46741 gtaaaccagc cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga
    46801 ggtgaagctc aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa
    46861 aatcatgatg ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg
    46921 caggatgttc attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa
    46981 tgcagtcatg acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc
    47041 gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt
    47101 taccgcttcg tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac
    47161 aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat
    47221 tgatttttcc cgcgagcgat aagagatcat catcgggctt aacgtctgga taaacagaat
    47281 cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg
    47341 tagcgaaccg acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac
    47401 taccagcagc ttggtcagca ccatgtcggt cgaaattttg aataacaatt tctcgccgcg
    47461 tgaagagatc gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag
    47521 caacccttcc gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt
    47581 ggaatagacg aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc
    47641 ccagttttta cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc
    47701 tgccagcgta ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga
    47761 tgagagcaaa aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc
    47821 cgcgccaatc gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt
    47881 gatcgcttct cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc
    47941 tgcaatgacg cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc
    48001 ttattatttt tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt
    48061 tattagaata aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagacttta
    48121 aaataacgga gatggataag aattttctac taaattaatc gcaagagaat attcattctc
    48181 tatttgcggc agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac
    48241 agcaatgtca gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt
    48301 tatcgttcat atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac
    48361 gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc acggcattaa
    48421 tggcattacg ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc
    48481 caaggttatg ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca
    48541 gtagttcagt taaccctgcc gctgccattg aacaggccac gccaatctcc ccctgacagc
    48601 cgacctccgc gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg
    48661 ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag
    48721 ccagcactgc cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc
    48781 cagctgcgtt ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca
    48841 tcggatcgtt agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac
    48901 gcggcacatt gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt
    48961 catgcatcac ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg ctgcgcagcg
    49021 ccagctcgtt gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca
    49081 gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc
    49141 cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata
    49201 gcgcttcctg tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt
    49261 cgggatggaa gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca
    49321 ctggcagccg cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa
    49381 catcctgcgg actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt
    49441 tgcccgtcag tgacaacgac ccgtacagat cgaccacaat atggctcgtc gcggttaata
    49501 agccgctact ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat
    49561 gcgaactgga gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac
    49621 cctcggattg ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctaacgag
    49681 gctttacgca ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg
    49741 cgcaacctgc acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt
    49801 atagacttca ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat
    49861 gatatcgccc acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa
    49921 acgcgcctga tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg
    49981 cccggaggtg aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc
    50041 tagggcacgt tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg
    50101 taaaagtacg tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg
    50161 cgtagccaga gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca
    50221 gcagcatttc ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt
    50281 cgtggtggaa atacccatcc agcaggccga caaggttggt tttacgtact ggatcttctt
    50341 tgcccagcgc cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga
    50401 acggcagttt cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca
    50461 tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg
    50521 ttttctggcc gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt
    50581 tgcgataggt tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa
    50641 tgctgtctac gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt
    50701 ccaccgccag gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca
    50761 gggagtccgt cgccaccgac aggcccgcga tgccgcatgc catagtgcga tagacatcac
    50821 gatcgtgcag cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga
    50881 tattcagcgc gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca
    50941 tcactttgtc gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa
    51001 tcttcagctt ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc gccaggttag
    51061 cgcgtgcacc aaagaactgc atttgcttac caatcaccat tgggctgacg cagcaggcaa
    51121 tcgcgtaatc gtcgctgttg aagtcagtac gcatcagatc gtcattttca tactgcaagg
    51181 aagaggtgac gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg
    51241 accaaagaat ggtcaggtta ggttccggtg ccggccccat agtgtgcagg tgtgcaaata
    51301 gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc
    51361 cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac gcaggaagcg
    51421 taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct gctcattgag
    51481 gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg tgcgccccag
    51541 cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt agagccactg
    51601 caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat
    51661 ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt
    51721 ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt gcagttcgcg
    51781 ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg
    51841 cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat
    51901 atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag
    51961 atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt
    52021 aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca ggccaacaat
    52081 tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt
    52141 ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacttt
    52201 ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat cgccttcata
    52261 cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc agtccgtacc
    52321 tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat ctaccttcat
    52381 gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac
    52441 tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacgac
    52501 gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt tagatcgatt
    52561 attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga ccagacgacg
    52621 aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca gccgacgtaa
    52681 tgaagctgca tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag
    52741 ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag
    52801 accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct
    52861 ctggttggtt tggctggcga tccaggacat cgcaccaaag tcgacatcgc cactgcgggt
    52921 acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac tctgaccgtt
    52981 gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc
    53041 ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg
    53101 gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata
    53161 agcttccgga gccatcgtct ggtggaaact ggtatcaaat acggccacct gagttacgcc
    53221 cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat gcaggggtgc
    53281 cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt
    53341 aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt catttaaatt
    53401 ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt ggagagccag
    53461 cggtgctggc tctccaccat ttacggataa gaatgcattt tccgagttaa taccgtcggc
    53521 aatacctgac attaatactt cacagtcgct ggcatcgagt acggaaaact taatcgaaga
    53581 cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag
    53641 ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa
    53701 caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta cggatagcat
    53761 acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt
    53821 cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc
    53881 tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa ccgaacttca
    53941 gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa gatttgaaga
    54001 tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttcg
    54061 taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcactt
    54121 tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca aagaacatca
    54181 ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga
    54241 agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga
    54301 ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca gtgatcagga
    54361 taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt
    54421 tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca
    54481 ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca
    54541 gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc tggttttccc
    54601 agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg
    54661 cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg
    54721 tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc agcgcccggt
    54781 ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc
    54841 caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc
    54901 ctaacgtcca tgtggtatct gatttacgcc aggacgattg tttcgtctgg ctggatacaa
    54961 tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca acaaaaccgg
    55021 tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc
    55081 tgttttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg
    55141 cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt
    55201 cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac
    55261 ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt
    55321 ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg ccaataacac
    55381 gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccaccaccac
    55441 caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg
    55501 ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata cgaccttcca
    55561 tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga
    55621 cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca
    55681 ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc
    55741 ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat
    55801 taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata
    55861 tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc
    55921 gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc agatcgtatg
    55981 taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg taactgacac
    56041 ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaataact aaccaacttc
    56101 tattaattgc cttcgtctac acccattata agatgaatac tctttggcta attccaccaa
    56161 aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac
    56221 aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat
    56281 atcacaagga attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac
    56341 tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag
    56401 ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc
    56461 cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc
    56521 gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc
    56581 aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat
    56641 agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt
    56701 aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcctcag aagacatacc
    56761 gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa
    56821 caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc
    56881 aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc
    56941 ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg
    57001 cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat
    57061 gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca ctcacaaatt aactttcatt
    57121 actttccaag gtatcgttat tacaattaaa tacctttaaa tatcaacaag ttaaagcata
    57181 aaaatcagca taaaccctga tttctatcaa aaaaaaatga cggggataac catattttat
    57241 ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc atatttcaag
    57301 caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa gttctcaaat
    57361 tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat tttttcacgc
    57421 tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa tacaggtatt
    57481 gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac gtcattttgt
    57541 gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag cattttgtgt
    57601 tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga agtcaacaag
    57661 ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc gtttactgta
    57721 acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga aaataagcat
    57781 ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc aattgcacat
    57841 atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa tagaaatatt
    57901 gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga cgttaataaa
    57961 atttacagta taagtaatga actgcggaga gtattatctg caattactgc attgaatttc
    58021 tatcaaggcg atgttccttc tgtcatgatc cgaatccaac cggaaaatat gagtccattc
    58081 atcatagata tttctacagg agaacatgat gattatatca tacaaacatt agatgtaggc
    58141 acttttgcac cttttggtga acaatgtact tgctcagccg tcaataaaaa agagctggaa
    58201 tgtattaagg agacgatttc taagtattgc gcaaagttca cccaaaaaga agccatctta
    58261 acacccccta ggcactttaa taaaacctgc attacttcag attgttggca aattctcttt
    58321 ttttcacccg atcattttaa taatgatttt tattgattat ttataaagga taaaataatg
    58381 ttccctgttt catcaattgg taatgatatt agtagtgatt tagtccgtcg aaaaatgaat
    58441 gaccttcctg aaagccccat aggaaataac ctcgaagcac tagcacccgg tatagaaaaa
    58501 ctaaaacaga cctctattca aatggtcact ttacttaacg cgttacaacc aagtggaaaa
    58561 tgcattatca ctggtgattt tcaaaaagaa ttagcgtact tacaaaatgt aattctttat
    58621 aatgactcgt ctcttcgtct ggatttttta ggttataacg cccaaattat tcaacgatcg
    58681 gacaatacct gcgaacttac cattaatgaa ccgttaaaaa accaggaaat atctacaggc
    58741 aatatcaatg ttaattgccc attaaaagat atttacaatg aaatcaggag gttgaacgta
    58801 atctttagtt gtgggactgg agatatagtt gatctatcct ctctggactt acgtaatgtc
    58861 gatttagatt attatgattt cacagataaa catatggcta atactattct aaatcatttt
    58921 aaattggatt caacaaattt tactaatgcc aacatgtttc aggttaattt tgttagttca
    58981 acacaaaacg ccacaatctc ctgggattat ttactaaaaa taacgcctgt tttaataagc
    59041 attagcgata tgtattctga agaaaaaatc aagtttgtcg aaagttgttt aaatgagctt
    59101 ggagacatta ccgaagaaca attaaaaatt atgagatttg caattataaa atctatacca
    59161 agggcaactc ttacagataa attagaaaat gaattaacaa aagaaatata taaaagctca
    59221 tcgaaaatca acaattgctt gaacagaatt aaattaccag agatgaaaga attctcatca
    59281 aaaaaaatat atgattacat cgatacaatc attgaagatt atgaaaatat caaagaaaat
    59341 gcttatctgg tcgtccccca aattaattat actatggatt taaacataga agactctagc
    59401 tcagaagagt taataccctc gagaaagacg aaaattctcc ggacaatggc tttgaggtcg
    59461 gggaatataa cacatatgaa gcatataact cagagaatca atattttacc agagaggact
    59521 atacgtataa ttacgacctt ttaaatgcaa tatagactta ataacttctt acaaagtcta
    59581 ttattaatac cgtagatatt catcttaccc tgtgcaccaa aagcaaaaac ttatcgacgc
    59641 gggtttgaaa ctgatggcaa ccgcaaaaat gcctgatgcg ctacgcttat caggcctacg
    59701 ccatctctgc aatatattga atttgcgtgc ttttgtaggc aggataaggc gttcacgcag
    59761 catccggcat gaataaagcg cactcaacaa tctgaaaccc gccggaacgg tttattacgt
    59821 acatcaggtg aaactgaccg ataagccgct ttcttttggg tatagtgtcg tggacagtca
    59881 ttcatctttc tgcccctcca aaagcaaaaa cccgccgaag cgggttttta cgtatatcag
    59941 gtgaaactga ccgataagcc gctttctttt gggtatagcg tcgtggacag tcattcatct
    60001 ttctgcccct ccaaaagcaa aaacccgccg aagcgggttt ttacgtacat caggtga
//
LOCUS       DADMFL010000012        59724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.012, whole genome shotgun sequence.
ACCESSION   DADMFL010000012 DADMFL010000000
VERSION     DADMFL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.012"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..158
                     /locus_tag="JNZ27_000722"
     CDS             <1..158
                     /locus_tag="JNZ27_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8663173.1"
                     /translation="HHVNLDKQDNRIKNIRLMPKEKHRRIHKNVRKLIRLGHTQWLQP
                     QLIEQSL"
     gene            176..439
                     /locus_tag="JNZ27_000723"
     CDS             176..439
                     /locus_tag="JNZ27_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663174.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            462..1004
                     /locus_tag="JNZ27_000724"
     CDS             462..1004
                     /locus_tag="JNZ27_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663175.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            1469..1675
                     /locus_tag="JNZ27_000725"
     CDS             1469..1675
                     /locus_tag="JNZ27_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663176.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            2134..2472
                     /locus_tag="JNZ27_000726"
     CDS             2134..2472
                     /locus_tag="JNZ27_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8663177.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            2453..2731
                     /locus_tag="JNZ27_000727"
     CDS             2453..2731
                     /locus_tag="JNZ27_000727"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663178.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            2724..4184
                     /locus_tag="JNZ27_000728"
     CDS             2724..4184
                     /locus_tag="JNZ27_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8663179.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            4206..5027
                     /gene="murI"
                     /locus_tag="JNZ27_000729"
     CDS             4206..5027
                     /gene="murI"
                     /locus_tag="JNZ27_000729"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8663180.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(5147..5589)
                     /locus_tag="JNZ27_000730"
                     /pseudo
     CDS             complement(5147..5589)
                     /locus_tag="JNZ27_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6113..7486)
                     /gene="eutA"
                     /locus_tag="JNZ27_000731"
     CDS             complement(6113..7486)
                     /gene="eutA"
                     /locus_tag="JNZ27_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY8663181.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSSLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(7461..7940)
                     /gene="eutP"
                     /locus_tag="JNZ27_000732"
     CDS             complement(7461..7940)
                     /gene="eutP"
                     /locus_tag="JNZ27_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY8663182.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7953..8288)
                     /gene="eutS"
                     /locus_tag="JNZ27_000733"
     CDS             complement(7953..8288)
                     /gene="eutS"
                     /locus_tag="JNZ27_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY8663183.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(8581..10860)
                     /gene="maeB"
                     /locus_tag="JNZ27_000734"
     CDS             complement(8581..10860)
                     /gene="maeB"
                     /locus_tag="JNZ27_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY8663184.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            11149..12054
                     /gene="tal"
                     /locus_tag="JNZ27_000735"
                     /pseudo
     CDS             11149..12054
                     /gene="tal"
                     /locus_tag="JNZ27_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            12044..13555
                     /gene="tkt"
                     /locus_tag="JNZ27_000736"
                     /pseudo
     CDS             12044..13555
                     /gene="tkt"
                     /locus_tag="JNZ27_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
     gene            complement(13658..14647)
                     /locus_tag="JNZ27_000737"
                     /pseudo
     CDS             complement(13658..14647)
                     /locus_tag="JNZ27_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
     gene            complement(14826..15401)
                     /gene="nudK"
                     /locus_tag="JNZ27_000738"
     CDS             complement(14826..15401)
                     /gene="nudK"
                     /locus_tag="JNZ27_000738"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY8663185.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(15469..17448)
                     /gene="aegA"
                     /locus_tag="JNZ27_000739"
     CDS             complement(15469..17448)
                     /gene="aegA"
                     /locus_tag="JNZ27_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY8663186.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17654..19371
                     /gene="narQ"
                     /locus_tag="JNZ27_000740"
                     /pseudo
     CDS             17654..19371
                     /gene="narQ"
                     /locus_tag="JNZ27_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            19573..22686
                     /gene="acrD"
                     /locus_tag="JNZ27_000741"
     CDS             19573..22686
                     /gene="acrD"
                     /locus_tag="JNZ27_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY8663187.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(22785..22844)
                     /gene="ypfM"
                     /locus_tag="JNZ27_000742"
     CDS             complement(22785..22844)
                     /gene="ypfM"
                     /locus_tag="JNZ27_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY8663188.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            23225..23581
                     /locus_tag="JNZ27_000743"
     CDS             23225..23581
                     /locus_tag="JNZ27_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY8663189.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23585..24712
                     /gene="dapE"
                     /locus_tag="JNZ27_000744"
     CDS             23585..24712
                     /gene="dapE"
                     /locus_tag="JNZ27_000744"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY8663190.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTIKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            24740..24940
                     /locus_tag="JNZ27_000745"
     CDS             24740..24940
                     /locus_tag="JNZ27_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY8663191.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANTPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(25050..25748)
                     /gene="ypfH"
                     /locus_tag="JNZ27_000746"
     CDS             complement(25050..25748)
                     /gene="ypfH"
                     /locus_tag="JNZ27_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8663192.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(25822..27837)
                     /locus_tag="JNZ27_000747"
     CDS             complement(25822..27837)
                     /locus_tag="JNZ27_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY8663193.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(27852..28715)
                     /locus_tag="JNZ27_000748"
     CDS             complement(27852..28715)
                     /locus_tag="JNZ27_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY8663194.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(28883..29596)
                     /gene="purC"
                     /locus_tag="JNZ27_000749"
     CDS             complement(28883..29596)
                     /gene="purC"
                     /locus_tag="JNZ27_000749"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY8663195.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(29809..30843)
                     /gene="bamC"
                     /locus_tag="JNZ27_000750"
     CDS             complement(29809..30843)
                     /gene="bamC"
                     /locus_tag="JNZ27_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY8663196.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(30860..31738)
                     /gene="dapA"
                     /locus_tag="JNZ27_000751"
     CDS             complement(30860..31738)
                     /gene="dapA"
                     /locus_tag="JNZ27_000751"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY8663197.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            31884..32456
                     /locus_tag="JNZ27_000752"
     CDS             31884..32456
                     /locus_tag="JNZ27_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY8663198.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            32456..32926
                     /gene="bcp"
                     /locus_tag="JNZ27_000753"
     CDS             32456..32926
                     /gene="bcp"
                     /locus_tag="JNZ27_000753"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY8663199.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            33179..33796
                     /gene="hyfA"
                     /locus_tag="JNZ27_000754"
     CDS             33179..33796
                     /gene="hyfA"
                     /locus_tag="JNZ27_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY8663200.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            33796..35716
                     /gene="hyfB"
                     /locus_tag="JNZ27_000755"
                     /pseudo
     CDS             33796..35716
                     /gene="hyfB"
                     /locus_tag="JNZ27_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            35727..36674
                     /gene="hyfC"
                     /locus_tag="JNZ27_000756"
     CDS             35727..36674
                     /gene="hyfC"
                     /locus_tag="JNZ27_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY8663201.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            36691..38130
                     /locus_tag="JNZ27_000757"
     CDS             36691..38130
                     /locus_tag="JNZ27_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY8663202.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            38142..38792
                     /gene="hyfE"
                     /locus_tag="JNZ27_000758"
                     /pseudo
     CDS             38142..38792
                     /gene="hyfE"
                     /locus_tag="JNZ27_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            38797..40377
                     /gene="hyfF"
                     /locus_tag="JNZ27_000759"
     CDS             38797..40377
                     /gene="hyfF"
                     /locus_tag="JNZ27_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY8663203.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            40367..42081
                     /gene="hyfG"
                     /locus_tag="JNZ27_000760"
                     /pseudo
     CDS             40367..42081
                     /gene="hyfG"
                     /locus_tag="JNZ27_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            42091..42636
                     /gene="hyfH"
                     /locus_tag="JNZ27_000761"
     CDS             42091..42636
                     /gene="hyfH"
                     /locus_tag="JNZ27_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY8663204.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            42633..43391
                     /gene="hyfI"
                     /locus_tag="JNZ27_000762"
     CDS             42633..43391
                     /gene="hyfI"
                     /locus_tag="JNZ27_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY8663205.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGYPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            43384..43797
                     /gene="hyfJ"
                     /locus_tag="JNZ27_000763"
     CDS             43384..43797
                     /gene="hyfJ"
                     /locus_tag="JNZ27_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY8663206.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            43827..45839
                     /locus_tag="JNZ27_000764"
                     /pseudo
     CDS             43827..45839
                     /locus_tag="JNZ27_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            45861..46709
                     /locus_tag="JNZ27_000765"
                     /pseudo
     CDS             45861..46709
                     /locus_tag="JNZ27_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(46747..47808)
                     /locus_tag="JNZ27_000766"
     CDS             complement(46747..47808)
                     /locus_tag="JNZ27_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8663207.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            48021..49484
                     /gene="bepA"
                     /locus_tag="JNZ27_000767"
     CDS             48021..49484
                     /gene="bepA"
                     /locus_tag="JNZ27_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY8663208.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARNLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            49505..49864
                     /gene="arsC"
                     /locus_tag="JNZ27_000768"
     CDS             49505..49864
                     /gene="arsC"
                     /locus_tag="JNZ27_000768"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY8663209.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(49997..50743)
                     /gene="hda"
                     /locus_tag="JNZ27_000769"
     CDS             complement(49997..50743)
                     /gene="hda"
                     /locus_tag="JNZ27_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY8663210.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(50793..52082)
                     /gene="uraA"
                     /locus_tag="JNZ27_000770"
     CDS             complement(50793..52082)
                     /gene="uraA"
                     /locus_tag="JNZ27_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY8663211.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(52168..52794)
                     /gene="upp"
                     /locus_tag="JNZ27_000771"
     CDS             complement(52168..52794)
                     /gene="upp"
                     /locus_tag="JNZ27_000771"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY8663212.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            53118..54155
                     /gene="purM"
                     /locus_tag="JNZ27_000772"
     CDS             53118..54155
                     /gene="purM"
                     /locus_tag="JNZ27_000772"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY8663213.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            54155..54793
                     /gene="purN"
                     /locus_tag="JNZ27_000773"
     CDS             54155..54793
                     /gene="purN"
                     /locus_tag="JNZ27_000773"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY8663214.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            54965..57031
                     /gene="ppk1"
                     /locus_tag="JNZ27_000774"
     CDS             54965..57031
                     /gene="ppk1"
                     /locus_tag="JNZ27_000774"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY8663215.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            57036..58577
                     /gene="ppx"
                     /locus_tag="JNZ27_000775"
     CDS             57036..58577
                     /gene="ppx"
                     /locus_tag="JNZ27_000775"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY8663216.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(58616..>59724)
                     /gene="pdeF"
                     /locus_tag="JNZ27_000776"
     CDS             complement(58616..>59724)
                     /gene="pdeF"
                     /locus_tag="JNZ27_000776"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8663217.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        14338 a        15400 c        15197 g        14789 t
ORIGIN      
        1 tgcaccacgt taatttggac aaacaggata accgcatcaa gaatattcgc ctgatgccga
       61 aagaaaagca ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac
      121 aatggcttca accgcaatta attgaacaat cactataaac acagtagtaa ataccatgca
      181 ttcattagac agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg
      241 tgaggaattc catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga
      301 aacggttcgt ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga
      361 tatggaaggc actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc
      421 aataagcgct tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac
      481 tggcaaataa aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac
      541 gcgcactgct aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta
      601 atgaagttat cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag
      661 ccgccggact ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgc
      721 acaaacagag cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg
      781 cgcaggttga ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg
      841 gagtggatag ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt
      901 ttgatgccgc cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg
      961 agcaacggaa acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc
     1021 cgggaattac cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca
     1081 ggataagcag tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca
     1141 tcatgttggc actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag
     1201 acctgaaaca ttcgccactg agcaaacagg ccatgaagcg ctaaacgctt cgtctgtggg
     1261 ctgaatattc gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc
     1321 agagcgcgga ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata
     1381 gccagttaca cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca
     1441 agtactatgc gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat
     1501 actcgaaggc cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag
     1561 gaacaacgaa gcagagttac taaccaaata catcagtttc accaattact gcatctacga
     1621 gtgttacaag gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga
     1681 ataacaccac gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt
     1741 tagtgtgcca cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct
     1801 ctcacatatg taggaataga ccaggttggc attgaaacac atctaccatg accataataa
     1861 agtaattttt tgatatgttt atggttttat attaattaaa atatatctaa aattgatagt
     1921 tttaaaattg caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtgccg
     1981 tttattatga aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc
     2041 tcacttgagc tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct
     2101 cgaaatgtat tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac
     2161 ttagctactt tctgtcaaac atggcttgat gatgaaatcc gtgagttgct taatttagct
     2221 attgataaaa acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc
     2281 tgtgtgcgta tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc
     2341 tcaaccattg gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg
     2401 tgtaaaaaac gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat
     2461 atgctggcat aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga
     2521 tcatcacctg ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg
     2581 tgtcgttacg actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa
     2641 gataagtgtt aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt
     2701 taaaaatatt cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa
     2761 tgacagtggg gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac
     2821 cttctgaagc agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt
     2881 ttttagtacc tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg
     2941 gggcatttcg atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta
     3001 tgacctggct acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg
     3061 ttgcatttat tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata
     3121 ttttattttt cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg
     3181 gtattttctc gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta
     3241 tccttgttat tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat
     3301 taggttgggg tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca
     3361 gcatatttct tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata
     3421 atccaactaa aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat
     3481 tcctgctcag tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc
     3541 aaagtttgtt ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct
     3601 ctgtgatggc atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag
     3661 ggcctaatac aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa
     3721 aaaccaatcg tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag
     3781 ttctttctgt tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac
     3841 agttaacagt tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc
     3901 tacgacattc tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg
     3961 caacacttgg attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc
     4021 aaatcccaac tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat
     4081 ttttattaat tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact
     4141 ctgatttcga accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac
     4201 aaaagatgag gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg
     4261 taggtggatt gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct
     4321 attgtggaga taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt
     4381 tgactaaaaa aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat
     4441 gtaatactat atcctcaact ttggaatccg aaaaatacag cggatatgct aagagctttc
     4501 cgtttcctat cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata
     4561 aaaatgttgg tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa
     4621 taaaaaaatt gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt
     4681 taattgagga aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta
     4741 accatttaat ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa
     4801 ttgttcagaa tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg
     4861 atcaggctat ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata
     4921 taggaacttt gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg
     4981 aattgagcat tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtgc
     5041 accgtaaaat cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt
     5101 gcattttatg gacaaaccca gttacatact gggtatgtgc atccaattag tagaacatgt
     5161 gtttttcgat aaacgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga
     5221 ttgttttacg acccaaccgc ttaatgcggg tgcgtagcgt cagattatta cgctcaatgc
     5281 gttgggtgaa tattttgccg gtcagatgct tattcttcgg cacctcccgg ccatagctgc
     5341 cccagtcatc gctggtcagc atgccgatgt tgaagggtgt aagcagtgcc agtagctccc
     5401 ggcaccatta aaggccattt cagttatcag ctctttcata cccggcttac gtgcttgata
     5461 agtataagtg aactgaaaca cacggtggca gtcacggcag cgaagtctgt cacggccttt
     5521 agggttctga ccatggcggt aaacctgagc tgactgacaa cggggacaat gaatattaac
     5581 gctggtcatg agataacctc aaaagcccgt attatacatc agattcaact aattagaggc
     5641 atcccccatc cagcattata aaacatacta acaataacta aattataata atcacaactt
     5701 acaatcaaat aataaatgca taagattaac aatgattttt tcgggttata tttatctaaa
     5761 aaagcagaat attatgtaac ttattatttt ctaagcgaaa atttattgtg cggtcagact
     5821 aacaaacttg cgtaccaccc gaagaacatg cgaagattta agtattcttt cttttttggt
     5881 ttttattttt cattttctct tttcttctct ttccggttct cttctgttct ggttttggtt
     5941 ttttcctttt tatttttatt ctttcttcag ggttatgcta aaacaccaaa acatttttca
     6001 ccacaaaagc atccaatcat cttgccagac caatcacctt aaccggatac actgccggat
     6061 ataatcccac taacacccag ccaccaagca ccatacctga taactaccgt attcaggaag
     6121 gaaatgcgag tgatttcacc gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa
     6181 tgtcgatata gtcccccgcg cggacaatca cttcgtcaat gactgccaac gggagttgtt
     6241 gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa gtcctgcccg gccaccacca
     6301 gcaggggatg cggattcgga aaacgcgcga cgaaatcgac cagcgcgttg atgaccgtca
     6361 gtaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc cagcacgtac acgtcagttt
     6421 tgggatcaag atccagctga agcagcgcct gttgccaggc actcaccaga tccgtttcat
     6481 caatcgggat cgccaccggc aaattgcgca gcggcagttg tacgccctcc agccagattg
     6541 tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat caccgtggcg catacggttt
     6601 gcgccggaaa ctgcacattc atctcacgca ggcgcggatg gtcatgcagc gccgttgcca
     6661 gaagtgggcc aatatcggca aaacagaacg ggtcggcggg ctggtggcga taacattcgc
     6721 ccacgcctcc agaaagcgta atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt
     6781 gcatcaatgc ctgcgcgagc gacgaaagcg ttccgtcaat cacttcgaca atcagcgccg
     6841 ccatccgccg ggtaacctgt accagctgcg cgccggtcag cgaacgggcg tcagtgcctg
     6901 caccgaagca ctcatccaca atcatctgcc ccggtttatg agcgtaaacc acgcgcccct
     6961 ggctgtcggt ttccagcagg cgaccaccga cgttgagaca ggcagtgccg ctgatttttc
     7021 cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat atcgatattc agtacccgac
     7081 acagccgttg ttcagaaagg gtttgcgccc cggctccgtg accggcgatc acggattcga
     7141 ggtgcggccc ggcgctggcg acgacaaaat cgcccagcga ctgtttttcc ttcatcactt
     7201 tttccatcag ctgtgcgacc aggctttcgg taaactggcc ttccggcagc tgggcaatga
     7261 tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg cacgctggcg ggcacggacg
     7321 ccgttaccgg gatcgattca tcacattcgg tgatggtaaa acccagtaga tccgacactt
     7381 cacgggcttc cggggtaata atgctggcgc gcagaaccac ggacattgcc tgttcgccgc
     7441 gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt tttcacctgc ttcctcctgt
     7501 ttggtgagag atgccagata atccaccagt tgctgcacgc tttgcgggtc gtggctgttt
     7561 agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata actttcgcgt tgcggcgacg
     7621 tcagcatctg gcatgtccgt tttactgatg acgacgattt gccgtttgct gacgccaata
     7681 tccagcaacc cggcaggcaa ccgactttcc gggtcatttg cgccatggac atagatcagc
     7741 atgtctacat cctgtagcgt ggtaattaag gcgtgatacc agcggggatg gctaaaatat
     7801 tcacccggcg tatcaatatc gcccttatca ttaaattcca cggcctgtgt ttttctggcg
     7861 agggtataat tcccctgcaa cgcattaaat aacgttgttt ttcctgcccc gacgctaccc
     7921 acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt tggtcatttc acaaagcgta
     7981 taatttaata agcgacctaa accgctgacg gtttgtgata acgcttcttc tactgcgccc
     8041 accgagccat aaatcaccaa tgcaccgctg aagcggtcga gaaaaccgat atgcacatca
     8101 gcggctttca gcgctaaatc accggcgatc atcgccgttt cgccgggagt tagcgtcata
     8161 atgccaattg cgcccgcatc cggaacgccg attttcttcg ccagttcctc accgggatgc
     8221 gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa attcctgaat gatgcgttct
     8281 ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc gtgacaattt ttcgtcagag
     8341 taaataacaa aattcgtcaa gcgcgtttaa aaggtgaggt agatcactaa gaatggagag
     8401 atatttactg ttatgaataa actttcatat gcgacgcatg aatataaata aaataatatc
     8461 gagatatttt atatactgat ttttttatta ttcgcaaact cagtaataag ctttgctaat
     8521 atatttcagg aaaaccgctt accctgaata ttcagggtaa gcgtgagagt taagaatgaa
     8581 ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc accatattga cgatacgacg
     8641 caccgatgcg atcggcgtta acacgtgaac cggtttcgcc acgcccatca gcaccggacc
     8701 gacagtcaca ccttccgagc tggaaacgcg cagtaagttg taactaatgc gggcagcttc
     8761 catgttcggc atcaccagaa tattggcaga acctttcaaa gggctgtccg gcatacggtc
     8821 gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt tcaccatcaa tcatcagatc
     8881 tggcgcacga tccctgacca gttccagcgc ctgacgcatt ttgctcgacg acgggcagtc
     8941 agaagaacca aagttggagt gcgacaacag agcaacgcgc ggctcaatac caaaacgacg
     9001 gacagtttct gccgccatca aggtgatctc cgccagctct tctgcgtctg gttcgtcatt
     9061 gacgtaggta tcggcaacaa aggtgttacc actcggcagc agcagcgcgt tcattgcacc
     9121 tgcggtgtga acgccatcgc gataaccaaa gacatttttc accacgctaa aatgttcatg
     9181 ataatcgccc accgtaccgc agatcattgc atcggcttcc ccacgctgaa ccatgatcgc
     9241 gccgatcact gtcgggttac tgatcagcgc acgctgcgcc tgttcctgag tgacaccgcg
     9301 acgcttcatg atctggaagt attcggtcca gtactcttta aagcgcggat cggattcgtt
     9361 attgacgatt tcaaaatcaa cgcccgcttt gatctgcaag cccagtttct gaatgcgcat
     9421 ttcgatcacg ttcggacgac cgataaggat cggtttcgcc agtcccagcg ttaccagttc
     9481 ctgagtcgca tgcagaacgc gcgcctcttc cccttccggc agaactacgc gcttcggcgc
     9541 tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg gttttgtaaa cgaactcagt
     9601 cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc gtcgccacgc ccgactccat
     9661 cgccgctttt gcgaccgcag gagcgatctt aacgatcaag cgcggatcaa acggttttgg
     9721 aatgatgtat tccggaccaa agctcagatc ctgaacgcca tacgctgaag ccaccacttc
     9781 actctgttct gcatgggcca gttctgcaat cgcgcgtacc gccgccagtt tcatctcttc
     9841 gttgatggcg gttgcgccaa cgtcaagcgc gccacggaag atgaacggga agcaaagaac
     9901 gttgttcacc tggttcgggt agtcggaacg gccggtacag atgatggcat ccggacgcac
     9961 ttctttcgcc agcggcggca gaatttccgg ttccgggttc gccagcgcca ggatcattgg
    10021 tgcccgagcc attttcttca ccatttcctg ggtcagcact ttcgggccgg aacagcccag
    10081 gaaaatatcc gcaccttcaa tcacatcatc gagggtacgt ttgccgtcat ccaccaccgc
    10141 atatgcggct ttggtttccg ccatatttgg ctcacgcccc tgatagataa cgccttttga
    10201 atcgcaaacc acgatgttat gtttttgcag ccccagcgct accagcaggt tcatacaggc
    10261 gattgctgcg gcacccgcgc cggaaaccac catccgcacg tcggagatgt ttttctccac
    10321 cacgcgcaag ccgttgagga tggcggcagt gctgatgatt gccgtgccgt gctgatcgtc
    10381 gtggaatacc ggaatattca tccgctcgcg cagtttctgt tcaatataga aacattctgg
    10441 cgctttaatg tcttcgaggt tgatgccgcc gaaggtcggt tcgagcgcgg cgacgacttc
    10501 aataaatttg tccgggtcga gttcatcaac ttcaatgtca aatacatcaa tcccagcgaa
    10561 tttcttaaat agaacgccct tgccttccat caccggtttg cctgccagcg cgccaatgtt
    10621 gcctaacccc agcaccgccg taccgttaga gatcaccgca accaggttac cgcgcgcggt
    10681 atatttgtag gcttttagcg ggtctttttc gatttcaaga caaggtgcgg caacgcctgg
    10741 tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc ttggttggag aaacctggat
    10801 tttccctgga actggaaatt catggaaatc aagtgcactt tgttttaact ggtcatccat
    10861 ttggttgttc ctttcacgta acgttcacaa ataaagtgtg tgggcaacag cccctgccca
    10921 caacgtggcg cacattatta ccctgccgga gtctacagac tttgagcaag tccaaactct
    10981 caccattaat ataatgtttt ggtaacaatc ctataacact gatgttacct gcttaatcca
    11041 gcaataccat gcctgtctgc tatgcttttt tgatgcgttt agcgaaattt ctcagaagtg
    11101 tgaattaacg cactcatcta acactttact tttcaaggag tatttcctat gaacgagtta
    11161 gacggcatca aacagttcac cactgtcgtg gcagacagcg gcgatattga gtccattcgc
    11221 cattatcatc ccctggatgc caccaccaat ccttcgctgt tactcaaggc tgccggatta
    11281 tcacaatatg agcatttaat agacgatgct atcgcctggg gtaaaaaaaa tggcaagacc
    11341 caggaacaac aggtggtcgc agcgtgtgac aaactggcgg tcaatttcgg tgctgaaatt
    11401 ctcaaaatcg tacccggttg cgtgtcaaca gaagttgatg cacgcctctc ttttgataaa
    11461 gaaaagagta ttgagaaggc gcgccatctg gtggacttgt atcagcaaca aggcgttgag
    11521 aaatcacgca ttttgatcaa actggcttcg acctgggaag gaattcgcgc ggccgaagag
    11581 ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt tttcttttgc ctaggcgcgg
    11641 gcctgtgcgg aagcaggcgt ttttctgatt tcgccgtttg tagggcgtat ttatgactgg
    11701 tatcaggcac gcaagccgat ggacccgtat gtggtggaag aagatccggg cgttaaatcg
    11761 gtgcgcaata tctacgacta ctataagcaa caccactatg aaaccattgt gatgggcgcg
    11821 agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct gcgatcgact gactatcgca
    11881 ccgaatttgc tgaaggagct gcaggaaaaa gtttctccag tggtacgtaa attaatcccg
    11941 ccttctcaga cgttcccacg cccagcaccc atgagcgaag cggagttccg ttgggagcac
    12001 aatcaggatg cgatggcggt agaaaaactg tctgaaggca ttcgtctgtt cgctggcagg
    12061 cacgctggga ctaggcaagc tgattggttt ttacgatcac aacggtattt ccatcgacgg
    12121 tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct atcactggca
    12181 tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc
    12241 gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt acggtgattg gctttggttc
    12301 gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag aagaagtggc
    12361 gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta aagagattta
    12421 tcacgcctgg gatgcccgtg aaaaaggcga aaaagcgcag cagagctgga atgagaagtt
    12481 tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttacccgac ggatgagcgg
    12541 tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt tacaggcaaa
    12601 tccggcgaaa atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt acgggccgat
    12661 gctgcctgag ttgctcggcg gttcggcgga tctggctccc agcaacctga ccatctggaa
    12721 aggttctgtt tcgctgaagg aagatccggc gggcaactac attcactacg gggtgcgtga
    12781 atttggcatg accgctatcg ccaacggcat cgcgcaccac ggcggctttg tgccgtatac
    12841 cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg cggcactgat
    12901 gaaagcgcgg cagattatgg tttataccca cgactcaatt ggcctgggcg aagatggtcc
    12961 gacgcaccag gctgttgagc aactggccag cctgcgctta acgccaaatt tcagcacctg
    13021 gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg agcgccacaa
    13081 cggaccgacg gcactgatcc tctcaaggca gaatctggcc caggtggaac gtacgccgga
    13141 tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg gtaagccaga
    13201 tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg cagagaaatt
    13261 agcaggagaa ggtcgcaatg tacacgtagt ttccctgccc tcgaccgata ttttcgacgc
    13321 ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc gcgtggcggt
    13381 ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg caattgtcgg
    13441 gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct ttggctttac
    13501 cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg cctgatggtg
    13561 atcgccggat gctgtatgtt cccctcaccc taaccctctc cccagaaggg cgaggggacg
    13621 gtccgtgcgc tttttagggc cgcatctgcg attttgttta acgggtgatc cacaacgtgg
    13681 gcctggcgtc tggcccatgc cagttatcgc aggtaggttc tgcggcgtaa cgcaccaggc
    13741 gaaaacgctg accgtcaaag cgtcagcgcg cctgaatgcc acaatcgctt aatccgcgcc
    13801 ctttcgctaa ggtcaccagt tcacgcgatt tctcatcaaa tgttgcgttc atcagttcca
    13861 gttcattcgt ctcctgaccg ttgttgaacg gcaaacgcaa ccgaaccggg cgcgaggcta
    13921 gcggcttttt acgcgacaca atccatgcca aatcaatggt gttataggcc cctgcctcac
    13981 agctaatcat catcagtgct ttatcatcgg tcagcgcagt gacattcacc tcacgacgta
    14041 acggatcaag cgagcagcgc agaccattca tccgccagtt tccataatcc agcaaatcgt
    14101 tacgttcttc gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc tctttcagcg
    14161 caggcgcggg cggtacgctg agaggcggtt cgtccccttt cttgatccac gcggtttcac
    14221 tgccaacgcg cttttgctga gcatcaataa acaacaacgc cgcttttaag ccgctcagag
    14281 aaatggtctg attgccgtcg cgtaaggtga tcgccttccc ttcctgaatt atctgcaaaa
    14341 acgcggtgat ggttgccgta tcatcggtta ctaataacca tggtgaaatc cgccacttgt
    14401 caccacttaa tgccaacggc tcaccatcta atagcagccg tggcgctatc tccccttcag
    14461 acgcatccgg cgacttcaac ccaccgcgct caatacgtaa aacggcatcg gtatgcgccc
    14521 cagcgctgcg gctcagggtc atcaccagtc cattatgatc gcccgtattg cgcgccacac
    14581 aaaaattttg attattacag gtgacctgcc agtcggaaaa cgcccgttgc gctggtgccg
    14641 cccacaccaa agacgtcggc aacaaagcga aaaaaagaga aggaaaatgc gatagcgcat
    14701 ggacggtacg accccagaag ttcaaacaaa cagcacaagt cgtatcttcg ttgcgcaggt
    14761 agcgcggctc aatcggattt gtcggattga gccaataata aacaacgaaa tattattgct
    14821 atttttcagt ccattaaatg tgacatttgc aaatagttaa gcaataacac cgtcttaccg
    14881 tcacgtatct cgccggtttt gatcatctcc agcgcctggc tgaacggcag ctcgagcact
    14941 tcaatatctt catcttcaac accgccaccg gcgttagcgc gctgattgtc actgtattcg
    15001 gcgataaaaa agtggattag ctcagtcaca ccgccaggcg acatatacag ttcaaataat
    15061 ttgcgcactt cgcccacttc atagcccgtc tcttcaatcg cttctttacg aatgcacact
    15121 tccggttcgt cgttatccag cagcccggcg caggtttcaa tcagctgccc gctttcattg
    15181 ccattaaccc aggtagcgac acggaactga cgaatcagaa ccacgctctt tttcttcgcg
    15241 ttgtacagga ggatcgtcgc accattaccg cgatcgtaaa cttcacgttt atggcggata
    15301 acttcaccat ctttgcgcgt gagatcgtaa gtaatgttgt gcagggtgaa atagttatcg
    15361 gagagaattt tgtctttaat gagggtgatt tgttgcgtca taccgactcc acagcgcgaa
    15421 atgaacaatt atattacgct gtgaagtcgg gtttgtctgc gcaggctatc agtgagattt
    15481 gactgatttt acccccagcc agtcaataat cccctgtgcc gcatgacgtc cttctgccat
    15541 tgcggtaacc accagatccg caccacgcac ggcgtcgcca ccggcgaaga ttttcggatt
    15601 ggtggtctgg taacggtact ggctttccac atccgcgatg atgcggcccc atttgtctac
    15661 cgttacaccg tgcgactcca gccacggcat cccgtgtgga ttgaagccaa acgccataat
    15721 caccgcatcg gctggcatga caaattcact gccctcaatc ggtacaggtc gccgacgccc
    15781 ctgggcatcc ggctcgccaa gacgcgtgcg caggaaacga attccgcaaa cgtgaccttg
    15841 ctcattcagc tcaagcgcca ccggctggac gttaaattcg aagttggccc cctcttcgcg
    15901 ggcgttcttc acctctttct tcgagcctgg catgttagct tcatcacgac gataagcgca
    15961 ggtgacgtta ctcgcgccgt ggcgcagcgc agtacgcaca cagtccatcg cggtgtcgcc
    16021 gccgcccagt accacgacgt taagtccggc ggtattgata aacggctctt ccggtagctc
    16081 ttcgagcccc atcacctgtt tagtgttggc aatgaggaac ggcagcgcgt cataaacgcc
    16141 cggcgtatct tcattgggta aacccgcttt catggaacgg taagtgccta cgccaacgaa
    16201 gaccgcgtcg tattgttcca aaagcgaatc caaagagaca tctttaccca cttcacaatt
    16261 gagttcgaag tgaatcccca tcgcgctgaa gatttcccga cggcgtgcca gcagggattt
    16321 atccagtttg aaagaaggaa tgccgaaagt gagcaagcca ccgatttctg gatggcgatc
    16381 gtacaccgtc accgccacgc cattgcgggt cagaacatcc gcacaggcca gccctgccgg
    16441 acctgccccg ataatcgcca cccgcttgtc cactttggtg acatgactta agtcaggacg
    16501 ccaacctttc gccaacgcct gatctgaaat atagcgttca atgttgccga tagttaccgc
    16561 gccgtgctca tcgcgaatag tacaggcacc ttcacacaaa cggtcttgcg gacaaacgcg
    16621 tccggtaatt tccggcaggg tgttggtctg atgagaaagc tcgacggcgg cgtcgatgtt
    16681 tccggctttc accagttcaa tccactgcgg aatatggtta tgcagcgggc aggtccattc
    16741 acaaacgctg tgctcgccga acttaaggca gcgcgaggct tcccgttgtg cctggtcggc
    16801 gcgaaatggc agataaattt catcaaaacc ggttttgcgc gcttcaatcg ccagtttatc
    16861 cggctcgcca cgcgcgggcg ttgcctgcat ttgttcgact ttactcatta ccggcatttc
    16921 ttgtgcagcg gtactggcat gccacggttg atgctcctga cgtgcggtgc gcaagcggcg
    16981 ggatttcgcc atgccggaga gtgcgacgtc agtgaccagt tgcagcgcgt ccgccgggca
    17041 attctcgaca caggcaggac cgttttcgcg ccctgcacaa aggtcgcatt tatgcgccgt
    17101 ggcttttact tttcctgccg cgacgggtgt caggacgatt tgcatcgtac caaacggaca
    17161 ggccaccacg caggatttac agccaataca cttttgctga ttgacctgaa tgctgtcatc
    17221 aacgtgactg attgcgccat tagggcagct acgggcgcag ggcgcatctt cacaatggtg
    17281 acaggtcact gcactacgtt gctgctgatg tttgataacc gtaattcggg gatgaaaatg
    17341 gtgttggctc aggacatgtt gctcatcatt gtgagccatg acacaggcga tttcacaagc
    17401 atgacaaccc agacattgct gactgttggc cataataaaa cgattcataa cgaccttctt
    17461 ttttggttgt aaaaacctta ttctttatat gagtgttgtt attacccgac ttacagggga
    17521 accggcaatg ttcatgtgcc cagaataagt aactatttcg ctataaactg tggcagatca
    17581 aataatccca tcactgacta aattgcgttt cagcgaactg gaacattaat gattttttgt
    17641 ggagaagacg cgtgtgattg ttaaacgccc cgtctcggcc agtctggccc gggccttttt
    17701 ttacattgtg ctgctgtcga ttctttccac gggtatcgct ctgctaactc tggcgagcag
    17761 tttgcgcgac gctgaggcta tcaatattgc cggatcgctg cgtatgcaga gttaccgcct
    17821 gggctacgac ttgcaaagtg gcagtccaca actcaatgca catcgccagt tgtttcagca
    17881 ggcactgcat tcaccggtat taaccaacct caacgtctgg tatgtgccag aagcagtaaa
    17941 aactcgctat gcgcatctga atgccaactg gctggagatg aataatcggc tcagcaaggg
    18001 cgatttgccg tggtatcagg ccaatattaa taattatgtt aatcagatag acctgttcgt
    18061 gctggcttta cagcactacg ctgaacgcaa aatgctgctg gtggtggcga tttccctggc
    18121 tggcggtatc ggtattttca cgctggtctt ttttactttg cgccgcatac gccatcaggt
    18181 ggttgccccg ctgaatcagc tggttaccgc cagtcagcgt attgaacacg ggcagttcga
    18241 ctcgccgccg ctggatacca gtctgccgaa tgagcttggt ctgcttgcaa aaacctttaa
    18301 ccaaatgtcg agcgagctgc ataaattgta cctttcgctg gaggcgtcag tagaagaaaa
    18361 gacccgcgat ctccacgagg ccaagcgtcg tctggaggtg ttgtatcagt gcagtgttcc
    18421 caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt gcagattgtt
    18481 cgcgacaatg aagcagctga atatctggag ctgaacgttg gggataactg gcggattagc
    18541 gaagggaagc caaaccctga gcagccaatg cagattttac cggtgacaat gcaagagacg
    18601 gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc gctgcttaac
    18661 agtgtttcat cgatgctggg acgcggtttg tactttaatc aggcgcagaa gcattttcag
    18721 cagttattgt tgatggaaga acgtgcgacc atcgcccgcg aattgcacga ctcgctggct
    18781 caggtgcttt cttacttacg tatccagttg acgttactga agcgttcgat accggaagat
    18841 aacgccaccg cacaaagtat catggccgat ttttcccagg cgttgaatga tgcttatcgg
    18901 cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga tctcccctcc
    18961 gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgctaaact gaccctcgac
    19021 tgccgtctgc caaccctggc actggatgcg caaatgcagg tgcatttgtt gcaaattatt
    19081 cgcgaagcgg ggctgaatgc gatgaagcac gccaacgcca gcgaaatcgc catcagttgc
    19141 gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat tggtatcggt
    19201 gaaccgaaag aacccgaagg tcattatgga ctgaatatca tgcgcgaacg cgcggaacgg
    19261 ctgggcggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt gagtattagc
    19321 tttcgctccg cagagggtga ggggaagggg aacacgccgg ctgaggtgta aataattacc
    19381 ctcacaccgg ccctctccct ttaaaggcga gagggaaaaa cgtgcccata ttgacctgta
    19441 agcgagtgtg tcagcaatct catgataatt tacattaact cctttttttc tccacgattg
    19501 gctcgtacct tgccgctaca gtgaagcaag tcaagcctac aacaatacgc agaaacacga
    19561 ggtcctcttt taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca
    19621 atcctgttgt gtctgacagg taccctggcg attttttcat tgcccgttga acaatacccc
    19681 gatctcgcgc caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg
    19741 ctggaaaaca ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctgatg
    19801 tatatgtcat ctcagagcag tggcaccggt caggcatctg tcactttaag ttttaaagca
    19861 ggcaccgatc cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga
    19921 aagttaccgc aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaac
    19981 attctgacca ttgccttcgt ctctaccgat ggctcgatgg ataaacagga tattgctgat
    20041 tatgttgcca gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat
    20101 gcctatggtt cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc
    20161 cagatgacgg ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt
    20221 gggcaacttg gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc
    20281 cagtcactgc tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaggac
    20341 ggctcagagg taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat
    20401 tatcttagcc gcttcaatgg taagccagcc tccgggttgg gggtaaaact ggcctccggc
    20461 gcaaacgaaa tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtat
    20521 ttcccgcacg gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc
    20581 attgaagacg tggtgaaaac gctgctggaa gctatcgctc tggttttcct cgttatgtat
    20641 ctgttcctgc aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg
    20701 atgggaacct tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc
    20761 gcgatggtgc tggcgatcgg tctgctggtg gatgacgcca tcgtggtggt ggaaaacgtc
    20821 gaacgtatta tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg
    20881 cagatccagg gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg
    20941 gccttcttcg gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg
    21001 gcgatggtgc tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacgcta
    21061 cttaagccac tgaaaaaagg tgagcatcac gggcaaaaag gtttttttgc ctggtttaac
    21121 cagatgttta accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt
    21181 agcctgcgct ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg
    21241 cgtttgccga cgtcgttctt accgctggaa gaccggggca tgtttactac ctcggtacaa
    21301 ttgcccagcg gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat
    21361 tacttcatcc atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct
    21421 gggggtaacg ggcaaaacgt ggcgagaatg tttatccgcc tgaaagactg gagcgaacgc
    21481 gacagtaaga ccggcacctc gtttgccatt atcgagcgtg caacgaaggc gtttaaccaa
    21541 attaaagaag ctcgcgttat cgccagcagc ccaccagcaa ttagcggtct tggtagttct
    21601 gcagattttg atatggagtt gcaggaccac gctggagcgg gtcacgatgc gctgatggca
    21661 gcacgtaatc agttactggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat
    21721 aacggcctcg atgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg
    21781 ctgggcgttg atatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat
    21841 gtgaatgact ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg
    21901 tatcgcatgc tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg
    21961 gtgcccttct ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc
    22021 tataacagtt attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt
    22081 acggcgatgg atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag
    22141 tggacggcga tgtcgtatca ggagcggctt tccggcacgc aggctccggc gctgtacgcc
    22201 atttccttgc tggtggtatt cctgtgtctg gcggcattgt atgaaagctg gtcggtaccg
    22261 ttctcggtaa tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg
    22321 cgcgggctgg aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg
    22381 gcgaaaaacg ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac
    22441 ctgtttgaag cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg
    22501 ctggcattta tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt
    22561 cagcatgcgg tgggtactgg cgtaatgggc gggatgattt cagctactat tctggctatt
    22621 tacttcgtgc cgctgttctt tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg
    22681 gaataagcaa taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag
    22741 aacctgctca gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata
    22801 acttcgataa agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata
    22861 attatgggta ttctacggaa aaaatatacc gtggtgaagc taatttactc gattgctgcg
    22921 atgactacct ccgggggaca aaccttatgt aaatactatg gtcctgcggt gataatttgt
    22981 atgtgataca cagcaacatt tcgagatatt catacggcat ctaatactta tttaattctg
    23041 gttaaaatac agacagataa caagatgaat attcttaatg tttacgttaa aaatgtttaa
    23101 tattatttaa tagttgttaa tttgaatact tcgataatgt tatattttct gataatcatt
    23161 tgcaggcaaa atgttttcac ccttaaatga gtatttattc tcataaatcg aaaaaggatt
    23221 cattatggtt acactttacg gcatcaaaaa ttgtgacacc attaaaaagg ctcgccgttg
    23281 gctggaagcc aataacatcg actatcgttt tcatgattac cgcgtcgatg ggctggacag
    23341 cgaattattg aacggtttta tcaacgaatt aggctgggaa gcgttactca acacccgtgg
    23401 tacaacctgg cgtaaactgg acgaaactac ccgcaataaa atcaccgatg cggcctctgc
    23461 ggcggcatta atgactgaaa tgcctgcaat tatcaaacgt ccattgctct acgcgcctgg
    23521 taagcctatg ctgctgggtt tcagtgattc cagttatcag caatttttcc atgaggtgta
    23581 gtctatgtcg tgcccggtta ttgagctgac acaacagctt attcgccgcc cttccctgag
    23641 tcctgatgac gcaggatgtc aggctttgtt gattgaacgt ttgcaggcga tcggttttac
    23701 cgttgaacgc atggattttg ccgatacgca gaatttttgg gcatggcgtg ggcaaggtga
    23761 aacgttagcc tttgccgggc ataccgacgt ggtgccgcct ggcgacgccg atcgttggat
    23821 caatccgccg tttgaaccca ccattcgtga cggcatgtta ttcgggcgcg gtgcggcaga
    23881 tatgaaaggc tcgctggcgg cgatggtggt agcagcagaa cgttttgtcg cacaacatcc
    23941 caaccatacg gggcgactgg catttctgat cacttctgat gaagaagcca gtgcccacaa
    24001 cggtacgata aaagtcgtcg aggcgttaat ggcacgtaat gagcgtctcg attactgcct
    24061 ggtcggcgaa ccgtcgagta tcgaagtggt aggtgatgtg gtgaaaaatg gtcgtcgcgg
    24121 atcgttaacc tgcaacctca ccattcatgg cgttcagggg catgttgcct acccacatct
    24181 ggctgacaat ccggtacatc gcgcagcacc tttccttaat gaattagtgg ctattgagtg
    24241 ggatcagggc aatgaattct tcccggcgac cagtatgcag attgccaata ttcaggcggg
    24301 aacgggcagt aacaatgtta ttccgggtga actgtttgtg cagtttaact tccgcttcag
    24361 caccgaactg actgatgaga tgatcaaagc gcaggtactt gccctgcttg aaaaacatca
    24421 actgcgctat acggtggatt ggtggctttc cgggcagcca tttttgaccg cgcgcggtaa
    24481 actggtggat gcggtcgtta acgcggttga gcactataat gaaattaaac cgcagctact
    24541 gaccacaggc ggaacgtccg acgggcgctt tattgctcgc atgggggcgc aggtggtgga
    24601 actcgggccg gtcaatgcca ctattcataa aattaatgaa tgtgtgaacg ctgccgacct
    24661 gcagctactt gcccgtatgt atcaacgtat catggaacag ctcgtcgcgt gatgagtggt
    24721 tctgcaagag gaaataagca tggactggct ggctaaatat tggtggattc tggtgattgt
    24781 ctttttggtc ggcgtcctgc tgaacgtgat taaagatctc aagcgcgtcg accataagaa
    24841 atttctcgcc aacacgccgg agcttccccc gcatcgtgat ttcaacgata agtgggacga
    24901 tgacgacgac tggccgaaaa aggatcaacc gaagaagtaa gctgagtgct caaccgtagg
    24961 ccggataagg cgttaacgcc gcatccggca atggtgaacg atgcctgatg cgacgctcac
    25021 gcgtcttatc aggccaggta atctgtcttt tacatcattt caatcacatc atcatcgcca
    25081 ggcttaccgc cacttagcgc ttggtcgaaa taatgtttcg gaatggtata acgcaagtga
    25141 tccagggcaa attgcatact gcgattgtcg atcgcatggc ctaaatcctc cacgatatcc
    25201 agcgtcacgt caccaccggc actgattaac gcttcctgcg ccgcaactgc atgcgccaga
    25261 tcgataaccg gatcttcacc accatgaatc aagtgaattg tcgtggctgt agacgcggtt
    25321 tccggcaggc tcgcatagcg tccgttaaag gcaatgacgc gcgaagcaag gcccggttcg
    25381 gctttaatgc tctccagcgc cataatcgcg ccttgtgaga aaccaattag cgccgtggcg
    25441 ttagccccta ctccgctctg tttctgccag tagcgtaccg tttcaataaa cgtcggcatg
    25501 atggcatcca cacgcgcctg gcgattatct tccgtaatac cctgcactga aaaccactga
    25561 cgccccgccg gattaccgct cggctccgcg ccaccgacac tcaccaccag cgcatcagga
    25621 aacagcggtg caaaccagct gcctatttcc cccattgcca ccgggttatc cccgacaccg
    25681 tggaaaagca gtaacaactg ttgtgcaggt ttatccgggc tttgaacaac aaaatggtca
    25741 tgtttcatgg cgaactcctt aactgatgtc attaatttta cgccgctgac tcacaatgac
    25801 catgggagaa aattgaagga gtcagtgaaa aaattgccat tgcgttatgc gatcgcgcag
    25861 acgctcagtg cgaacctcat ttagtgcgaa cagcgcctgc gcggcctctt cccgctggcg
    25921 aaccagtaac atcttgcggc ctgaaagttt aagtgtggta cataactgcg cgtcactggc
    25981 gtttttctgt aaacgcccac gcagcgccgg taatgccagt tcactggttt gtagcagacg
    26041 caataagcaa cctaacgatg ttaatagcgg acgatgagcg aaagcaaaac cggccagttc
    26101 aagccagtcg tcgtcagaaa ggatggcatc gtttagtgga tcaacaggaa gcatttcgcc
    26161 attccactgc gcgagagctt gcgcatcgcg acgtaaacgg taatgctcac gttcagccag
    26221 ctgtttaccc gcatcactca tcggtaacag cgccatcgcc gtatagcaac cgctgctggc
    26281 ttcccgatga ttacccatcc gcaccagcac aaaaccgcag cgttgccaga aacgccataa
    26341 ctccccagtg taaccaaaac tcaccgaaag atagtcgagg tcatgcgtat attgcaaagc
    26401 tccagcaata agttgccgcc ctgtgccttc ccgctgacga gccggatgaa ctgctatccg
    26461 gctaacccgc cttccgcgca atgtcgccgc cagtggattg ctgccgtgcg ccgccagcga
    26521 ctgggccacc agattacccc gcgggcgacg aaaacctgcc cataccgcct gactgagttc
    26581 ttgagataat ccgccctcat ccaccagcca caacgccccg gcaatctcgt tttcgccagc
    26641 cgcctgtaaa aaatgttgcc ctggtgcatc catcatccgg cgtaaatcca gcggcgaggt
    26701 ccggtagtgc gcaccagaca ataactgata aacctttaac ggcgtttctg gctcgcttcg
    26761 ccataacgtt tgttcaaatg cggaaatgac gatattgcct tgtggtgtat gggtgaagtt
    26821 ttcatcgtta aaaaccagtg cctcactaac cattttttcc agcgggcatc cctgtgccca
    26881 gcggatcggc tgttgcagtt caaaacggtg taaatgcgga aagcgagcgc aaaatttcag
    26941 caaaaaacca cgtccggtac cttcgtagcc ctgcaccgta gtggttaaca acgttcgagg
    27001 aaaacgcgat accagttgat gcaacaacgg cgcaggtatg gctgcggctt cgtcgaccac
    27061 cagccagtcg gcttgctcat cgctggctaa caaggcatcc ggtgcaataa agcgaaactt
    27121 ctcgcccgca aattgtgcca gtacatccgt tgccgctttt gcgggcgcgg tgacaatcgc
    27181 actgcccgca atacgagaaa tgagttgccc tgccaacgcc gacttaccgc gcccacgcgc
    27241 agccgttacc gctgccacgc ccggcggcat ggtcagtagc tgctgtaaga gttgctgttg
    27301 ttctggttgt ggtgcgccgg tcgcggggtg ccagtcagta cggggagtaa aatgcgccaa
    27361 cgagaacggc tggttttgcc gccagaggat agcgtcgtta tccgccgtaa gtacgcgttt
    27421 gagatgctgg acaaaatgcg gcgtcgcaat agggtcaggg caatcactcc agcgcagcga
    27481 gtcggcatca ggttggtttt cccactcttc ccatacaggg agtaacaaaa ccagccagct
    27541 tcccgctttc aacgttccgc taagtgctgc aaaggcagcg gcatcaaagc cgtggcgggc
    27601 gtcgaatacc gcatgccgga actcgcgccc aagtaaagtt tgtagcgccg agggtgaaca
    27661 gtggttttca gcatctagct gcggcgaaat ccacagccag tcgccaggta aggcatcacg
    27721 caacttaaga gcatgatcaa aacaccaacc ctcttctccg ctcaacacca gcaagcggca
    27781 gatcccttca cgtttcattt gcgctgttaa tgtgtaaagc gcagtcagtt cagccatccc
    27841 tgccccggaa attaaatgct tttaccaaaa gtattgcatt gtgccggatc gccgctgtcg
    27901 aaaccacgtt taaaccagct gcagcgttgc tgagaagtgc catgtgtgaa actgtctggt
    27961 actactcgcc cctgactttg ctgttgtaaa cggtcatcgc cgatggcctg cgccgcattc
    28021 agcgcctctt ccagatcgcc agtttccaga acgccttgct gctgcatact atgcccccag
    28081 acaccggcaa aacagtcggc ctggagttcc atacgcacag ataagcggtt tacttccgcc
    28141 tgcgtcgcgt tttgttgcag ttgacgaact ttcggctcga tgcctaacag tttctgcaca
    28201 tgatgaccga cttcatgggc gataacgtac ccctgggcaa aatcgccatc cgcgccaagt
    28261 ttgtctttca tgtcatcata gaaggagaga tcgatataaa ctgtgccatc cgccgggcaa
    28321 tagaacggcc ccataatgga ctggcccgcc ccgcagccgg tgcgcgtcat tccacggtac
    28381 atgaccagct tcggttgctg ataggtctta cccatctttt cgaactgttg tccccaggtg
    28441 tcttccgtgg ttgccagaat caccgaggtg aattttgcgg cttcgtcttc attagggcta
    28501 attgaccgcg ttgattgttg ttgagaaacc ggctgcccgg tcatcaaccc ggttaaatca
    28561 acgccatagt agcctgcaac cagcacgact atcagtaaaa tcagcccgcc tttaccgctc
    28621 ggcagacgga aaccgggacc gcccattgac ggaccgccag agctgttgcg cctgtcttca
    28681 acattgtcac tttcacgtcg cccttgccaa cgcatagaaa cctcaacaat atattcatta
    28741 tagaaatgat cgtaggtggt tcagaggaag attaccacag gaaagcggga tgaaatggcg
    28801 gaggaatgag cgttcaatgc atatattccg agagcggttg tcttgccgtg ccagctgcac
    28861 ggcaagatga tgaacagaaa aatcagtcca gctgtacacc caggcggcgg gcgacggctt
    28921 cataggcttc gatcaggcca ccgagactct ggcggaaacg gtctttgtcc attttctcca
    28981 gtgtttcttt gtcccacagg cggctgccgt ccggggagaa ctcatcgccc agtaccactt
    29041 cacctttgta cagaccaaac tccagcttga agtcgaccag aatcagacca gcatcatcga
    29101 acagtttttt cagcacgtcg ttcgctttgt aggtcagctc tttcatacgc gccaggttct
    29161 ctttgctcac ccagccaaag gtttcgcagt aagattcgtt gaccatcgga tcgtgcatgg
    29221 catcgttttt caggaacaga tcgaacagcg gcgggttcag ctcaatacct tcttcgattc
    29281 caagacgttt caccagagag ccagcagcac ggttacgcac gacacactca accggcacca
    29341 tatccagctt tttcaccaga cattcggtat cggagagcag acgctccatt tgagtcggga
    29401 tacccgcttc agccagtttg ctcataatga agtagttgaa cttgttgttc aacatacctt
    29461 tgcgatcaaa ctgctcaatg cgtgcgccat cccctgctga cgtatcattg cggaattcga
    29521 gcaccaacag gtccgggttt tccgtgctgt atacggtttt cgctttacca cgatacaact
    29581 cagcttgctt ttgcatcttt atcactcctg ggtgtgaatt aacgttttaa aatcttttgc
    29641 tgtctggtgt gccggatgtt ttttcggatg cggcgtgaac gccttatccg aactacgtcc
    29701 gacaaacaaa tttcgtgcga attaccgcta aaatcgcgtt ttcctgccga cgcacacgtt
    29761 tgcgtatcat atcagaaaaa agggccggat gattccagcc ctgtattttt acttgctaaa
    29821 cgcagcctgg aagacagcta ccagcgcgtc gttctgactc tgagtcagag tatgaccttt
    29881 cggatcgatg aactgtaggc tgctgcggtt atctaaatcg ccaacctgca gtttatagtc
    29941 accggatgcc aggcctggat cgctcgcgcc cagttcctgc cagtcgctgt cagacagcgg
    30001 cttataagtt acggccatgt tgccctgcga acgggtgctg tcggtcactt tcatgcccac
    30061 tttttccagc gacgctggca gacgttgcca aaccacattg aacggcccgc gtacgaccag
    30121 cattggtaaa ccggtgtcat cagctgcact ttgtacgtcc atagtggtgg aggcacgatt
    30181 ttgcgcagcg ttcgcggcgt cagtggcaga tttatccaga ccggcggaaa taacgttcat
    30241 catctccgtg ctgtaacgct gcatggaagc cgcgtctgca accggtttgc ccgcctgttc
    30301 caggttcagc agtttaaccg taaccgcctg ctgataaccc tgcggcttaa cagagatttg
    30361 ataacgacca cgatactgct cgtcttcgtc cagacggttc cattgtaccc aatcgatggt
    30421 cagtgtctga ccagcatcat cacgttgggt gatggtgtag tttttcgcct gcagcacgct
    30481 aaccacctgc ggccacagag tattgccacg accattttcc accagcaatg aagcggtatc
    30541 gcccgtgaac tgggtacgcg cgccagaaac cagtgccagc ggctgggctg gtggacgaat
    30601 gtccagcgcc ttaccgacag caccactacc gttggtcacc gggattgcat aatcaccgga
    30661 ggtcaccggc aaaatcattc cagccggggc atgaagctcc gcaagcggtg ccgcttccag
    30721 gtaggcttca tcaccactga cctgacgctt atagcgtgag tcagaactac aggcagcgag
    30781 taataaaaca agcgaaacac ccgcaacctt tgccaggcgc gacttttgaa cagagtaagc
    30841 catcaaatct ccctaaactt tacagcaaac cggcatgctt aagcgccgct ctgaccgtct
    30901 cacgaccact gtcggtgatt ggtgtcattg gcaggcgcag cgtatcggtc gccacaagac
    30961 ccagttcctt acatgcccat ttcaccggga ttggattggg ttcgacaaat agtttgttgt
    31021 gtaatggcat cagacgctga ttaataacgc gtgcctcggc aaaatgccct tctgctgcca
    31081 gtttgcacat ctgggccata tcacgcgctg cgacgttagc cgtaacggaa ataaccccat
    31141 gaccgcccaa ttgcatgaag tccagcgcgc tcgcatcatc gccgctcagc agaacaaaat
    31201 catctgaaac cagctctttg atctggttta cacgcgttaa gttccctgtt gcctctttga
    31261 ttccgataat attttttact ttcgccagac ggcccaccgt ttccgggagc agatcgcagc
    31321 cagtacggga cggcacatta tacagaattt gcggcaggtc agtatgctca gcgatggctt
    31381 tgaaatgctg atacaaacct tcttgcgacg gacgattgta gtaaggggtt accgtcaggc
    31441 agccgacgat accactgtca ttgaagcgct gcgtcaggct aatggcttcc gcagtagcgt
    31501 tagcgccggt cccggcaatt accggaatgc gcccatcagc cagatccagc gtcatcatca
    31561 ccacatcagc atgttcgtca tgatttaagg tagcggactc gccagtggtg ccaacagaaa
    31621 cgatcgccga agtaccgctg gcgacatgat aatcaatcag ttttttcaag ctagcccgac
    31681 agacattacc tttttcatcc atcggagtaa caatcgcgac aatacttccc gtgaacatgg
    31741 gccatcctct gtgcaaacaa gtgtctcaat ggtacgtttg gtatggcatc aaaagcaagc
    31801 agacagaacc gttctgattg ttgtatgcat gattttttta tgctttcctt aagaacaact
    31861 caccccttaa aggaataacc agtttgacac tgtcatcgca acattatctg gtgatcactg
    31921 cgttgggtgc cgatcgccct ggaattgtga acaccatcac ccgtcatgtc agtagttgcg
    31981 gctgtaatat tgaagacagt cgcctggcga tgctgggaga agagttcacg tttatcatgc
    32041 tgctttccgg ttcatggaat gccatcactc tgattgaatc aacgttaccg ttgaaaggtg
    32101 ccgaactgga tcttttaatc gtgatgaagc gcacgacggc gcgtccgcgt ccgccaatgc
    32161 cagcatctgt ctgggttcag gtcgatgttg cagactcccc gcatttaatt gaacgcttca
    32221 cagcactttt cgacgcgcat catatgaaca ttgcggagct ggtgtcgcgc acacaacctg
    32281 ctgaaaatga acgggctgcg cagttgcata ttcagataac cgcccacagc cccgcatctg
    32341 cggacgcagc aaatattgag caagcgttca aagccctatg tacagaactc aatgcacaag
    32401 gcagtattaa cgtcgtcaat tattcccaac atgatgaaca ggatggagtt aagtaatgaa
    32461 tccactgaaa gccggtgata tcgcaccgaa atttagcttg ccggatcaag acggagaaca
    32521 agttaatttg accgacttcc agggacagcg tgttctggtt tatttctacc cgaaagccat
    32581 gacccccggc tgtaccgtac aggcctgcgg cttacgcgat aacatggatg agttgaaaaa
    32641 agcgggcgtt gatgtgctgg gtatcagcac cgataaaccc gaaaaactct cccgttttgc
    32701 ggaaaaagag ctgcttaact ttacgctcct gtctgatgag gaccaccagg tgtgcgaaca
    32761 attcggcgtc tggggtgaaa agtccttcat gggcaaaacc tacgatggta ttcatcgcat
    32821 cagcttcctg attgacgctg atggcaaaat cgaacatgtc tttgacgact tcaaaaccag
    32881 caatcaccac gacgttgtgc tgaactggct gaaagaacac gcctgattac tttgctccat
    32941 tccgtgctgg ctgcgcttgc ggccagcata cctcacttct cgtgatcaag atcacattct
    33001 cgctttcccc tgcgacacgg gtgtcgaatc cattttttgc tgaacgttaa tgaccatcat
    33061 ttttgtaccg ttcagaatcc agttaataca taacttattg aatagattga gttaatcaga
    33121 atggcaccct ttatgcaata tgaaatgcaa tgtttcatat cattttcaag gagccgacat
    33181 gaaccgcttt gtggtggccg aaccactgtg gtgtacagga tgtaatacct gtctcgctgc
    33241 ctgttcggac gtgcataaaa cgcaaggttt acagcaacac ccgcgcctgg ccctggcgaa
    33301 gacgtcaaca atcactgccc ctgtcgtgtg tcatcactgt gaggaagccc cttgcctgca
    33361 ggtctgcccg gtcaatgcca tctctcagag ggatgatgcg atccaactca acgaaagcct
    33421 ctgtattggc tgcaagcttt gcgccgtggt ctgcccattt ggcgcaatca gcgcttcagg
    33481 aagccgtccg gtgaatgccc atgcgcaata tgtttttcag gctgaaggct cactcaaaga
    33541 cggcgaagaa aacgtgccgc cccaacatgc gttgctgcgc tgggaaccgg gtgtccagac
    33601 cgtcgcggtg aaatgcgacc tgtgtgattt cttgccagaa ggtccggcct gcgttcgcgc
    33661 ttgcccgaat caggcgttac ggctgatcac cgacgatagc ctgcaacgtc agatgaaaga
    33721 aaaacagcgc cttgccgcaa gctggtttgc caatggcggg gaggatcccc tttccctcac
    33781 tcaggagcaa cactaatgga tgccctgcaa ttattaacct ggtcgctgat cctctatctg
    33841 tttgccagtc tggcttcgct gtttttactc ggtctggaca gactggctat taagctttcc
    33901 ggcatcacat cgctggtggg cggcgtgatt ggcatcatca gcggaattac gcaattacat
    33961 gctggtgtaa ctttagtcgc ccattttgcc accccttttg actttgccga tttaaccctg
    34021 cgaatggata gcctctcggc atttatggtg ctggttatct ccttgctggt ggtggtttgt
    34081 tcgctctatt cattgactta tatgcgcgaa tacgagggca aaggcgcggc ggcgatgggc
    34141 ttctttatga atcttttcat cgcatcgatg gttgccctgc tggtgatgga caacgctttt
    34201 tggttcatcg tgctgtttga aatgatgtcg ctgtcttcct ggtttctggt cattgccagg
    34261 caggataaaa cgtcgatcaa cgctggcatg ctctactttt ttatcgccca cgccggatcg
    34321 gtgctgatta tgatcgcctt cttgctgatg gggcgcgaaa gccgggatga tgccgttgca
    34381 cagctggttg ccgcgcgctc accctgccgc accatcgcac gcttcggcgt tgatgtctgg
    34441 cgtaatggtc aaaatcggta ttttcggcat cctgaaagta gcgatggatc tgctggcgca
    34501 aacgggtttg cctctgtggt ggggcattct ggtgatggcg atcggcgcaa tctccgcgct
    34561 cctgggcgtg ctatatgcgc tggcggaaca ggatatcaaa cggctgctgg cctggagtac
    34621 cgtcgaaaac gtcggcatta ttttgctggc agtcggtgtg gcgatggacg gtctgtcact
    34681 gcacgacccg ctgctcaccg tggttggact gctcggcgca ctgtttcatc tgctcaacca
    34741 tgcgctgttc aaagggctgc tatttctcgg cgcggacgcg attatttcgc gtttgcatac
    34801 ccacgacatg gaaaaaatgg gggcactggc gaaacggatg ccgtggacag ccgcagcatg
    34861 cctgattggt tgcctcgcga tatcagccat tcctccgctg aatggtttta tcagcgaatg
    34921 gtacacctgg cagtcgctgt tctcactaag tcgtgtggaa gccgtagcgc tacaacttgc
    34981 gggtcctatt gctatggtaa tgctggcagt cactggtggg ctggcagtaa tgtgcttcgt
    35041 caaaatgtac ggtattactt tctgtggtgc gccgcgcagt acacacgctg aagaggcaca
    35101 ggaagtgcca aatacgatga tcgtcgccat gctactgctc gcggcactct gcgtattaat
    35161 tgcgcttagt gccagttggc tggcaccgaa gataatgcat attgcccatg cgtttaccaa
    35221 tacccctccc gtcactgtcg ccagcggaat agcacttgta cccggcacgt ttcatacacg
    35281 ggtcactcct tcattactgt tgctgttact actggcgatg cctttgctgc caggccttta
    35341 ctggctgtgg tgtcgttcgc gccgcgcagc gtttcgtcgc acaggagatg cctgggcatg
    35401 cggctacggc tgggaaaacg cgatggctcc gtcaggcaat ggcgtgatgc agccgctgcg
    35461 tgtggtcttc tctgcgttat ttcgtctacg acaacagctc gaccctacgc tgaggctaaa
    35521 taaaggtctt gcgcacgtca ccgccagggc tcagagcaca gaacccttct gggatgagcg
    35581 ggtgatccgc cccatcgtga gcgccaccca acggctggtc aaagaaatac agcatctgca
    35641 aagcggcgac tttcgtctct attgcctgta tgtggtcgcc gcactggttg tgctgctaat
    35701 cgctattgcc gtctaaggaa atcaccatga gacaaactct ttgcgacgga tatctggtca
    35761 tttttgcgtt agcacaggcc gtgattctgc tgatgctaac cccacttttt acgggtattt
    35821 cccggcagat acgcgcgcgt atgcactccc gccgcgggcc ggggatctgg caggattatc
    35881 gcgatatcca caaactgttt aaacgccagg aagttgcgcc gacatcttca ggtctgatgt
    35941 tccgcctgat gccgtgggta ttaatcagca gcatgctggt gctggcgatg gccttaccac
    36001 tatttattac cgtttcccct tttgcgggcg gcggcgatct gctcaccctt atctatcttc
    36061 ttgccctgtt tcgttttttc tttgctcttt ccgggctgga taccggaagt ccgtttgcgg
    36121 gagtcggtgc cagtcgcgag ttgacgctcg gcattctggt cgaaccaatg cttattctct
    36181 cactgctggt attggcgctg atagcaggtt ccacgcatat cgagatgatc agcaatacgc
    36241 tggcgatggg ctggaactcg ccgctaacca ccgtactggc gttactggcc tgtggttttg
    36301 cctgcttcat tgagatggga aaaattccct ttgatgttgc tgaagcagaa caggaattac
    36361 aggaaggccc gctgaccgaa tattccggtg ccgggctggc gctagcgaaa tgggggcagg
    36421 ggctgaaaca ggtcgtgatg gcagcactgt ttgtggccct gtttctgccc tttgggcgcg
    36481 cgcaagaact ttctctcgcc tgcctgctga cttcacttgt cgttacgctg ctcaaggttt
    36541 tgctgatttt tgtactggcc tcaatcgcag aaaacacgct ggcacgcggg cgttttttac
    36601 tcattcacca tgtgacctgg cttggcttca gccttgctgc gcttgcatgg gtcttctggt
    36661 taaccggtct gtaaggagca ctgacggaat atggaaaatc ttgctctgac gacgttaatg
    36721 ctgcctttta tcggcgcact ggtcgtttcg ttttcgccac aacgtcgggc cgccgaatgg
    36781 ggggttttgt tcgccgcgct gaccacgctg tgcatgttgt cgctgatctc cgcgttttat
    36841 caggccgata aagttgccgt cacgttgacg ttggtcaacg tgggggatgt ggcattgttt
    36901 ggcctggtca ttgatcgcgt gagtacgctg attctgtttg tggtggtgtt tctcggtttg
    36961 ctggtcacga tctactccac gggttatctg acggataaaa atcgcgaaca cccgcataac
    37021 ggcacgaatc gttattacgc atttttgctg gtgtttatcg gcgcgatggc gggactggta
    37081 ctctcctcga cgctgctcgg tcagttgttg ttttttgaaa ttacgggcgg ctgctcctgg
    37141 gcgttgatca gttattacca gagcgataaa gcgcagcgtt cagcactaaa agcgttgctt
    37201 atcacccata tcggttcgtt agggttgtat cttgccgccg ccacgctgtt tttgcagacc
    37261 ggaacgtttg tgcttagcgc gatgagcgag ttacatggcg acgcacgtta tctggtttat
    37321 ggcggcatcc tgtttgccgc gtgggggaaa tcggcccagc taccgatgca agcgtggcta
    37381 ccggacgcaa tggaagcgcc aacaccgatc agcgactatc tccacgccgc atcgatggtg
    37441 aaagtgggcg tttacatttt tgcccgcgcc attatcgacg gcggcaatat cccgcatgtg
    37501 attggcggcg ttggcatggt catggcactg gtcaccattc tttatggctt tctgatgtat
    37561 ttgccacagc aggatatgaa gcggttgctg gcctggtcga ccatcactca acttggctgg
    37621 atgttcttcg gcttgtcgct ctccatcttc ggctcgcggc tggcgctgga gggtagcatc
    37681 gcctacatcg tcaaccacgc gttcgctaaa agcctgtttt tccttgtagc aggtgcgctg
    37741 agttacagct gcggcacgcg cttgttgccg cgtctgcgtg gcgtattgca caccctgccg
    37801 ttgccaggcg tgggtttctg cgtggcagcg ctggcaatta ccggtgtgcc gccgttcaac
    37861 ggcttcttca gtaaattccc gctgtttgcc gccggttttg cgttgtcagt ggagtactgg
    37921 atcctgctgc ccgccatgat tctgctgatg attgaatcgg tcgccagttt cgcctggttt
    37981 attcgctggt ttggtcgcgt cgtgccgggc aaaccgagcg aggccgtcgc cgatgccgca
    38041 ccgctgccag ggtcaatgcg cctggtgttg attgtactga ttgtgatgtc gctgatttcc
    38101 agcgtaatcg ccgcgacctg gttgcagtaa ggagatgatg aatgaccggt tctatgatcg
    38161 taaataatct ggcgggactg atgatgctga catcgctgtt tgtgattagc gtcaaaagct
    38221 atcgcctgtc atgcggattt taggcctgcc agtcgctggt gctggtgtct attttcgcca
    38281 ctctctcgtg cctgttcgcc gcagagcaac tgctgatctg gtccgccagc gcctttatca
    38341 ccaaagtgct gctggtaccg ttaatcatga cttacgctgc acgaaatatt ccccagaaca
    38401 tcccggaaaa agcgttattc ggtccggcaa tgatggcact gctcgcggcg ttaattgtcc
    38461 tgctttgcgc atttgtcgtt cagcccgtga agctaccgat ggctaccggg ctgaaaccgg
    38521 cgctggcggt agcgttaggt cattttctgc ttggcctgct gtgcattgtc agccagcgca
    38581 atatcctgcg gcaaattttt ggttactgcc tgatggaaaa cggctcccat ctggtgctgg
    38641 cgtttcttgc ctggcgagca ccggaactgg tggaaatagg tatcgctacc gacgccatct
    38701 tcgccgtcat tgtgatggtg ttactggcaa gaaaaatatg gcgtacccac ggcacgctgg
    38761 acgtgaacaa cttgaccgcg ctgaagggat aatgagatga gttattctgt gatgttcgct
    38821 ttactcctgc tcacgccgct gcttttttcg ctgctctgtt ttgcctgccg gaaacggggg
    38881 ctctctgcga cttgtacggt gactgtatta catagcttag ggatcacact gctgctgatt
    38941 ctggcactct gggtggtcca aactgccgct gatgcaggag aaatattcgc tgcgggactg
    39001 tggcttcata ttgatggtct gggcggtttg ttcctcgcca ttcttggtgt gattggcttt
    39061 ctcaccggtg tttactcgat tggctacatg cgtcatgaag tggcacacgg cgagctttca
    39121 cccgttacac tgtgcgatta ctacggtttc ttccatctgt ttttgttcac catgctgctg
    39181 gttgttacca gcaataacct gattgtgatg tgggcggcga tcgaagccac caccttaagc
    39241 tcggcgtttc tggtaggcat ttacggtcag cgttcatcgc tggaatcagc atggaagtac
    39301 atcattattt gtactgttgg tgtcgctttt ggtctgttcg gtaccgtgct ggtatacgcc
    39361 aacgccgcca gcgttatgcc gcaggcagaa atggcgatat tctggagcga ggttcttaag
    39421 caatcgtcct tgcttgaccc aacattaatg ctgttggcct ttgtgtttgt gctaattggc
    39481 tttggtacca aaaccgggct atttcccatg cacgcctggc tgccggatgc tcacagtgaa
    39541 gcgccgagtc cggtcagcgc cctgctctcc gccgtattgc tgaactgcgc gctgttggtg
    39601 ctgattcgct attacatcat tatttgccag gccatcggca gcgatttccc caaccggttg
    39661 ttgctcatct tcggcatgtt gtcggttgcc gtggcggcat ttttcattct ggtacagcgg
    39721 gacattaagc gtctgctggc gtactccagc gtggagaaca tggggctggt cgcggtggcg
    39781 ctaggcattg gcgggccgct gggaattttt gccgcgctgc tgcacacctt aaaccacagt
    39841 ctggcaaaaa cgctgctgtt ctgcggttcc ggcaatgtac tgctcaagta cggcacgcgc
    39901 gatctcaacg tcgtctgtgg gatgctcaaa atcatgccat ttaccgccgt gctgtttggc
    39961 ggcggtgcgc tggcgctggc agggatgccg cccttcaaca tttttcttag cgaatttatg
    40021 accgttaccg caggactggc acgtaatcac ctgctgatta tcgtcctgct gttattgctg
    40081 ttaacgctgg tgctcgcggg cctggtacgg atggctgcgc gggtgttaat ggcgaaaccg
    40141 ccgcaggccg ttaaccgggg tgatctcggc tggttgacca cctcgccaat ggtgattctg
    40201 ctagtcatga tgctggcgat gggaacacat attccacaac ctgtcatcag gatcctggcg
    40261 ggcgcttcca ctatagtcct ctcagggacg cacgacctgc ctgcacaacg tagcacctgg
    40321 catgattttt tgccttcagg caccgcatct gtttcggaga aacacagtga acgttaattc
    40381 atcgtcaaat cgtggcgaag cgattctcgc cgccctgaaa acgcagttcc ccggcgcggt
    40441 gctggatgaa gagcgacaaa cgcctgaaca ggtcaccatt acggtgaaaa tcaatctgct
    40501 gcctgacgtt gtacattatc tttattatca acatgatggc tggcttccag tcctgtttgg
    40561 caacgacgag cggacactta acggtcatta cgcggtttat tatgcccttt caatggaagg
    40621 ggccgaaaaa tgctggatcg tggtgaaggc actggtcgat gccgacagtc gggagtttcc
    40681 gtcagtcaca ccgcgcgtcc ctgccgcggt ctggggcgag cgagaaattc gcgatatgta
    40741 cgggctgatt ccggttggcc tgccggatca gcgtcgcctg gtgttgcccg atgactggcc
    40801 ggaagatatg catccgctgc gcaaagatgc gatggattat cgactgcgcc ctgaaccgac
    40861 gacagattcc gaaacgtatg cgtttatcaa tgagggcaac agcgatgcgc gggtgatccc
    40921 tgtcggcccg ctgcatatca cctcctatga accgggtcac ttccgcttgt ttgtggatgg
    40981 cgagcaaatt gtcgatgctg attaccgcct gttttatgtc catcgcggca tggagaaact
    41041 ggcagaaacg cggatgggct acaacgaagt gaccttctta tcggaccgcg tgtgtgggat
    41101 ttgcggtttt gcccacagtg tggcctatac caactcggtt gaaaatgcac tggggattga
    41161 ggtgccgcaa cgagcacata ctattcgctc gattctgctg gaagtcgagc ggctacacag
    41221 tcatttgctc aaccttggcc tctcctgcca ttttgttggt tttgataccg gctttatgca
    41281 atttttccgc gtgcgggaaa agtcgatgac gatggcggaa ttgctgaccg ggtcgcgtaa
    41341 aacctacggt ctgaatctga ttggtggtgt tcgccgcgat attctcaaag agcaacgtct
    41401 gcaaacgctg aaactggtgc gcgagatgcg cgccgacgtg tcggagctgg tagagatgct
    41461 gcttgctacg ccgaatatgg aacaacgcac tcagggcatt ggcattctcg accgacaaat
    41521 cgcccgtgat tatagccctg tagggccgct gatccgcgca gtggttttgc ccgtgatttg
    41581 cgctttgatc acccctacgc cgactacggc aatattccaa aaacactgtt tacctttacc
    41641 ggcggcgatg ttttctcccg cgtgatggtc cgtgtcaaag agacgtttga ttcgctggca
    41701 atgctggaat ttgccctcga caacatgccg gataccccac tgctgaccga aggctttagc
    41761 tataaacctc acgcattcgc gctgggcttt gttgaagcgc cacgcggtga agacgtgcac
    41821 tggagcatgc tcggtgataa ccaaaaattg ttccgctggc gctgtcgtgc cgccacctac
    41881 gccaactggc cggtgttgcg ttacatgctg cgcggcaata ccgtttctga cgcaccgctg
    41941 attatcggta gccttgatcc ctgctactcc tgtaccgacc gtgtgacgct ggtagatgtg
    42001 cgcaagcgcc agtcaaaaac cgtgctgtat aaagagatcg aacgctacgg cattgatcgt
    42061 aaccgttcgc cgctgaagta aggacagaag atgctgaagt tactgaaaac tattatgcgc
    42121 gccggaaccc cgacggtgaa atatcccttc gcgccactgg aggtcagccc aggctttcgc
    42181 gggaaaccgg acctgatgcc cagccaatgt attgcctgcg gtgcctgcgc ctgtgcttgt
    42241 ccggcaaatg cgctgactat ccagaccgac gaccagcaaa attcgcgcac ctggcagctc
    42301 tatctggggc gttgtattta ctgcggacgt tgtgaagaag tgtgcccgac cagagccatc
    42361 cagcttacca ataactttga actgaccgtc acccataaag ccgatctcta tacccacgcg
    42421 acgttccatc tacaacgttg cagccgttgc gaacgcccgt ttgccccgca aaaaaccgtc
    42481 gcactggctg ctgaattgtt ggcacagcaa caaaatgctc cacaaaaccg cgaaatgtta
    42541 tgggcgcaag cgagcgtctg cccggagtgc aaacaacgcg cgacgctgat caacgacgat
    42601 acagatgtac cgctggtggc taaggagcag ctatgagtcc agtgcttacg caatatgtca
    42661 gccagcccat cacgctggac gagcaaacgc aaaagatgaa gcggcatttg ctacaggata
    42721 tccgtcgctc ggcttacgtt tatcgcatcg attgcggcgg ctgcaacgcc tgtgaaatcg
    42781 aaatttttgc tgccatcacc cccgtattcg acgcagaacg ttttggcatt aaggttgttt
    42841 catcaccgcg tcacgccgat attttgttat ttactggcgc agtcacccgg gcgatgcgta
    42901 tgcctgcact tcgggcgtat gagtctgccc ccgatcataa aatctgtgtt tcctacggcg
    42961 cgtgcggtgt cggcggcggt attttccacg atctctacag cgtctggggc ggcagcgaca
    43021 ccattgtccc cattgatgtt tggatccccg gctacccgcc aacaccggcc gccaccattc
    43081 acggtttcgc cgtggcgctc ggtttgctgc aacagaagat tcacgcggtg gattatcgcg
    43141 atcccaccgg ggtgactatg caaccgttgt ggccgcagat cccgccatca cagcgtatcg
    43201 ccattgagcg agaagcgcgg cggctggcgg gctatcgtca ggggcgagaa atttgcgatc
    43261 ggctcctgcg ccatttaagc gacgatccta caggaaatcg ggttaacacc tggttgcgcg
    43321 atgccgacga tccacgtctc aatagtatcg ttcagcaact ctttcgcgta ctccgggggt
    43381 tacatgactg aagagtgcgg ggaaattgtt ttctggacgc tgcgaaaaaa gtttgtcgcc
    43441 agtagcgacg agatgccgga acacagctct caggtaatgt attactcgct ggctatcggt
    43501 catcacgttg gcgtgattga ttgtctgaat gtcgccttcc gctgcccact gacggaatac
    43561 gaagattggc ttgcactggt cgaagaggag caagcccgac gtaagatgct gggggtgatg
    43621 acttttggtg agattgttat tgacgccagc cacaccgccc tgttgacccg ggcattcgcg
    43681 ccactggcag atgacgcgac gtctgtgtgg caagcgcgta gcattcaatt cattcatctg
    43741 ttggatgaaa ttgtgctgga accggctatc tatctgatgg ccagaaaaat tgcgtgagaa
    43801 ggatttctca ttaataagga ctgttgatgg ctatgtcaga cgaagcgatg tttgcccccc
    43861 cgcaaggaat aacaattgaa gcggtaaacg gaatgctcgc ggagcggtta gcgcagaaac
    43921 acggcaaggc gtctttatta cgcgccttca tcccgctgcc gccgccgttc agcccggtac
    43981 aacttattga actgcatgtt ctcaaaagca acttctatta ccgctaccat gatgatggca
    44041 gcgatgtgac ggcaacaaca gagtatcagg gcgagatggt cgattattcg cgtcacgccg
    44101 tccttctcgg cagtagttga atggcggagc tacgctttat tcgtacccac ggcagccgtt
    44161 ttactcccca agattgcaca ctgtttaact ggctggcacg cattatcacc ccggttctgc
    44221 aatcatggct caatgatgaa gaacagcagg tggcgctgcg tttgctggag aaagatcgcg
    44281 atcatcatcg ggtactggtg gatatcacta atgcagtgct gtcacatctt gatctcgacg
    44341 atctgatcgc tgacgtcgct cgtgagatcc agcatttttt cggtctggct tcagtcagta
    44401 tggtactggg cgatcatcga aagaacgaga agttcagcct gtggtgcagc gatctttctg
    44461 cctcacattg tgcgtgtctg ccacacaata tgcctggcga cagtgtattg ctgacacaaa
    44521 cgctacaaac ccgacaaccg accttgacgc accgtgcaga tgatctgttt ctctggcaac
    44581 gcgacccgtt attactctta cttgcatcta acggctgcga atctgcgctc cttataccgc
    44641 ttacctttgg caaccataca ccgggtgcat tgttgctggc gcatacctct tccactctct
    44701 ttagtgagga aaactgccag ctactacaac acatagccga tcgcatcgct attgccgttg
    44761 gcaatgccga tgcctgccgt agaatgaccg atttgcagga aagtttgcag caagaaaacc
    44821 accagcttag cgagcagctc ctttcgaatc tgggcgtcgg tgacattatc tatcaaagcc
    44881 aggcaatgga agacctgctc cagcaggtag atattgtggc gaagagcgac agtacggtgt
    44941 tgatttgcgg tgaaaccgga accggcaaag aggtgatcgc cagagcgatc catcaactta
    45001 gcccgcgaca cgacaagccg ctggtcaaaa tcaactgcgc tgccatcccc gccagtcttc
    45061 tggaaagtga gttattcggt catgacaaag gggcgtttac tggtgcgatt aatacccatc
    45121 gtggtcgttt tgaaattgcc gatggcggca cgttgtttct cgatgaaatt ggcgatctgc
    45181 cgttagaact tcagcctaaa ctgctgcgcg tattgcagga acgggagatt gagcgtctcg
    45241 gcgggagtag aacgatcccg gtaaatgtca gagtcattgc cgccaccaac cgtgatttgt
    45301 ggcaaatggt tgaagatcgc cagtttcgca gcgatctctt ttatcgcctg aatgtcttcc
    45361 cactggaatt gcctccgctg cgcgaccgtc cggaagatat ccctctttta gcaaagcatt
    45421 tcacgcaaaa aatggcgcgc catatgaatc gttcaattga cgccatcccg accgaggcgc
    45481 tacgccagtt gatgtcgtgg gactggccgg gcaacgtgcg cgagctggaa aacgtgattg
    45541 agcgagcggt actgctgact cgcggtaaca gtctgaattt acatcttaat gtccgacaaa
    45601 gccgtttact gccgacgcta aatgaagatt cagcgcttcg cagttcaatg gcgcagttgc
    45661 tgcacccgac gacgccagag aatgacgaag aagaacgtca gcgcattgtt caggtattgc
    45721 gagaaaccaa tggcattgtt gccgggcccc gtggcgcggc gacacgatta gggatgaagc
    45781 gcaccacgct gctgtcacga atgcagcgtc tgggaatctc ggttcgcgag gtgttgtaat
    45841 ctgcttttgc aggagtatgc atgagaaaca aactctcttt cgacttgcag ttgagcgcca
    45901 gaaaagcggc aatcgctgaa cggattgccg cccataaaat tgcccgcagt aaagtgtcgg
    45961 tctttttaat ggcgatgtcc gctggcgtgt ttatggcgat cggatttact ttttaccttt
    46021 ccgttatcgc cgatgccccg tcttcatagg cattaaccca tctggtgggc ggcctttgct
    46081 ttacacttgg ctttattttg ctggcggttt gcggcaccag cctgttcacc tcgtcggtaa
    46141 tgacggtaat ggcaaaaagt cggggcgtta ttagttggcg aacttggctg attaacgcac
    46201 ttctggtggc ctgcggtaat ctggcaggta ttgcctgttt cagtttgtta atctggtttt
    46261 ccgggctggt gatgagtgaa aacgcgatgt ggggagtcgc gattttacac tgcgccgagg
    46321 gcaaaatgca tcatacattt actgaatctg tcagcctcgg cattatgtgc aatctgatgg
    46381 tttgcctggc gctgtggatg agttattgcg gacgttcgtt atgcaacaaa atcgtcgcca
    46441 tgattttgtc catcaccctg tttgtcgcca gtggctttga gcactgtatc gccaatttgt
    46501 ttgtgattcc gttcgccatt gccattcgcc atttcgcccc tacctccttc tggcaactgg
    46561 cgcacagtag cgcagacaat tttccggcac tgacggtcag ccattttatt accgccaatc
    46621 tgctcccggt gatgctgggt aatattatcg gcggtgcagt gctggtgagt atttgttatc
    46681 gggctattta tttacgtcag gaatcctgaa aaatcagccc ggcgaaacag ttcgtcgggc
    46741 tgaagattat tcttgcgcga tttgcccatc gggccaggcg tggatcacgg ctttgatcag
    46801 cgtcgccaat ggaatggcga agaatacgcc ccagaatccc cacaaaccac cgaagatcac
    46861 caccgataaa ataatcacca gcggatgcag gttaaccgct tcggagaaca acaccggtac
    46921 tagcaggttg ccgtccagcg cctgaataat cagatacacc gcgaagcagc tccagaattc
    46981 agtacctgcg ccaaactgga atagcgccac gccaaccacc ggaatggtca ccacaaatgc
    47041 gccaatgtac ggaataagaa ccgagaagcc gaccagcact gccagcagca gcgaatagtt
    47101 cagcccgaag agcaagaacc ccagccaggt ggcgatcccc accacgatca tctccagcac
    47161 tttgccgcgg atatagttgg tgatttgttg attcatctcc ttccacacct gtcctgccag
    47221 cccacggtta cgcggcagca cccggcgaac ggcgttcagc atctgctctt tgtctttcag
    47281 caggaagaag accatcaatg gcaccagcac cagatagacg gctatggtca gcaaaccgac
    47341 cagcgaggcg agggaaattt tcaccaccga atcgcccatg gtcagcatcc gactgcgcat
    47401 attttcggcc attgcatcaa taatgcccgc gtccattaac accggatagc ggcgcggcaa
    47461 cgtggcggca aagtcagaaa gcttattgag catccccggc atatcgcgga ttaagtagat
    47521 gccctgttgc caggcgatag gcagtaccac gaacgccatc agtagcaata taccgacaaa
    47581 aaccaccaat acaatcgacg tcgcccagcg gcgggagcag ccaatagatt gcaggcgcac
    47641 ggttggccat tccagcaaat aagccagcac aatagccacc agcaacggag caagcaggcc
    47701 actaaagaaa aagataatgc caaatccggc aactaaaata accagcaagg caatcgcttc
    47761 cgggtcgcta aaacggcggc gataccattg catcaacatt tcgagcataa agaccttccc
    47821 tgaacctcaa gagcgggatt gcgatccgca attgtatcga aatgtcacaa aaaagacttc
    47881 gctttttatg acggattcag gaaactgaaa agtcatttga gtgggctaat cttcgccgtt
    47941 acactcaaag gcggcgaggt gggaacgata tttcacagta tcggtcaaat gactatcttc
    48001 agaaatacag gatagaggtt atgttcaggc agttgaaaaa aaacctggtt gcaaccctca
    48061 ttgctgctat gaccattggt caggtagccc cggcgtttgc cgacagcgca gacaccttgc
    48121 cggatatggg aacctccgca ggaagcacgc tttccattgg tcaggaaatg cagatgggcg
    48181 actattatgt ccgccagcta cgcggcagcg cgccgttaat taatgacccg ctgttaacgc
    48241 aatatattaa ttcgctgggg atgcgtctgg tttcgcatgc caattcggtt aagacaccgt
    48301 ttcatttttt tctgatcaac aacgacgaaa ttaacgcctt tgctttcttt ggcggcaacg
    48361 tggtgctgca ctctgccctg ttccgttatt ccgataacga aagtcaactg gcttcagtta
    48421 tggcgcacga aatctcccac gtcacccaac gtcacctggc gcgagcgatg gaagatcaac
    48481 aacgcagcgc gccgctgacc tgggtcggcg cgttaggttc tattttactg gcgatggcca
    48541 gtccgcaggc ggggatggcg gcgctgaccg gtacactggc gggaacgcgc caggggatga
    48601 tcagtttcac ccagcaaaat gaacaggaag cggaccgcat tggtattcag gtgctgcaac
    48661 gctcgggatt cgatccacag gcgatgccga ccttcctgga aaaattgctc gatcaggcgc
    48721 gttactcctc gcgcccgccg gaaattttat tgactcaccc gttgccggaa agtcgtctgg
    48781 cagatgcccg caaccgtgct aatcagatgc gcccgatggt ggtgcagtcg tcggaagatt
    48841 tctatctggc gaaagcgcgc acactgggga tgtataattc cggacgtaac cagctcacca
    48901 gtgatttgct ggatgaatgg gcgaaaggaa acgttcttca gcaacgagcg gcgcaatatg
    48961 gtcgtgcttt acaggcgatg gaagccaata aatacgacga ggcgcgtaaa acgctgcaac
    49021 cgttactggc ggcagaacct ggcaacgcat ggtatctcga tctggctacc gatatcgatc
    49081 ttgagcaaaa caaagccaat gaggcaatca atcgcctgaa aaatgcccgc aatttacgca
    49141 ccaatccggt gttgcagctc aacctggcga acgcttatct gcaaggcggt caaccacaag
    49201 aagcggccaa tattctgaat cgctacacct ttaataataa agatgacagc aacggctggg
    49261 atttgctggc acaggcggaa gccgcgctaa ataaccgcga tcaggagctg gctgcgcgag
    49321 cagaaggtta tgcgctcgcc ggacgactcg atcaggccat ttcgctgttg agtagcgcca
    49381 gttcgcaggt gaaattaggc agcctgcaac aagcgcgtta cgatgcgcgc atcgaccagt
    49441 tgcgccagct gcaggaacgc tttaagcctt ataccaagat gtaattcgca ttaaaggaag
    49501 aacaatgacc aaacaggtaa aaatctacca taacccacgc tgttcaaaga gccgggaaac
    49561 gctgaacctg ctgaaagaaa acggcgtgga gccagaagtg gtgctctacc ttgagacacc
    49621 cgccgatgcg gcaacgctgc gcgatttgct gaaaatgctg gggatgaata gcgcccgtga
    49681 attgatgcgc cagaaagagg atctttataa agaactaaac ctggctgaca gttcgcttag
    49741 cgaagaggcg ctgattcagg caatggtgga taatccgaag ctgatggagc gcccgattat
    49801 ggtcgcgaat ggcaaagcgc ggattggtcg tccaccggag caggtactgg aaatcgtggg
    49861 ttaagatgtg ctccatcgct agttgaagca cattgccgga tgcgacgctt gccacgtctt
    49921 atccggccta cgaatactat ggattaactt cgcgtaggtc aggcgttcgc gccgcatccg
    49981 acaataaaca ccttatctac aacttcagaa tttctttcac aaacggaatg gtcagcttac
    50041 gttgcgcggt aatcgacgca cgatccaact gatccaacgt cataaatagc gtgcgcattt
    50101 ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc cggcagttca aaaccacgca
    50161 aacgcgcgcg taactgtagc gcctgcaact tatcttcatc agaaagtggc tgcaatttgt
    50221 agatctgccc ccagtcgaga cgcgacgcga gatccggtaa tcccagattc aactgccgcg
    50281 gtggacgatc gccggtgatc aacaaccgtg ttttgcccga ttccagaatt cgattgtaga
    50341 gatcgaaaat cgccatctcc cacaactcat cgcctgcaat acactcaatg ttgtcgatac
    50401 agaccagcga caaatgctcc ataccgtcga gcacttccgg aacaaaccag gtgcgtttat
    50461 ccagcgggac atagcccacc gcatcgccac gctgcgacaa ttccgcgcaa gccgcgtgca
    50521 gcagatggct gcgccccgcg ccttcgcgtg cccagagata gatgtaaccg ctatgttcct
    50581 gacgcagcac gttttgcagc gcggccagta aagaggagtt atcccccggc cagaaacttg
    50641 caaaggtttc gtcgtcagga agataaagtg gcaaagagag ctgtgccggt gtgttcagag
    50701 atacctcaac caggatttca caaaatcgcg agaagtttac cacagaatcc catgatgttt
    50761 gaaccgggca gcaacactgc ccggtcggta cattatttgt ctgttatatc cgcgtcttct
    50821 gcgtccagca ccacttcttc agggcgcagc acactgatca gtttgaaaat caaacttaag
    50881 ccgataccga cgatggtcgc cagcgccata ccttttaact ccgccgcgcc gatgtttacc
    50941 ttcgcgccac tgacgccgat gatcaaaatc acggaagtca ggatcaggtt ctgtgctttg
    51001 ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg aagcaccaat gacaccataa
    51061 agcagcagcg aaacgccgcc catcaccggc aatgggatca tctggatagc ggcagccagt
    51121 ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc cgccgataac ccaggtactg
    51181 taaacacggg tgatcgccat cacgccaatg ttttctccgt aagtggtatt tggcgtagag
    51241 ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa acatcgaacg gtgcagacct
    51301 ggatcgcgca gcagatcttt tttgacgata ttagccgtta ctaccaggtg ccctacgtgt
    51361 tcggcaataa ccactaacgc cgctggcaga atagtcagaa tggcaaacca ctcgaagcgc
    51421 ggcgtataga gggttggcag tgcaaaccag tgagcattaa taatcggcgt ggtatcgaca
    51481 attcccattg cgaaagagag cgcgtacccc accagcacgc caattaaaat cgggataatt
    51541 gccaggaaac cacgaaacag cacggaacct aaaaccgtga ccgccagggt ggtaatagag
    51601 atgatgatgg ttttggagtt tggcgtttgc ccttcagccg ggagtaaacc cgccataccg
    51661 gcagctacgc ccgccagctc cagaccgatg acggcaacga ttgcgcccat tgccgcaggt
    51721 ggaaacagca cgtccaacca gccggtcccc gctttcttca cgataaaaga aaccaggcag
    51781 aacagcacgc cgcacataat aaagccgccc agcgcgactt cataccctaa cggcaacagt
    51841 aacaataccg gtgaaataaa ggcaaagctg gaaccaagat aagccggaat tttcccttta
    51901 cagatgaaga gatacagcag cgttccaata ccgttaaata acagtacagt cgccgggtta
    51961 atatgaaata agacgggcac caggacggtt gcaccaaaca tggcgaacaa atgttgcaaa
    52021 ctaagcggga ttgtctgtaa aagtggcggt ctttcactca ccccgatagc acggcgcgtc
    52081 atagtattat cctctgtatt atgtgttata ggcgctttac tcaaaaaaaa gccgactctt
    52141 aaagtcggct ttaattattt ttattcttta tttcgtacca aagattttgt caccggcatc
    52201 gccgaggccc ggaataatgt atccgtgctc gttcagtccc tggtcaatcg atgcagtata
    52261 cagttcgacg tccgggtgcg ctttttccag cgcagcgata ccttctggcg cagcgaccag
    52321 caccagaact ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac
    52381 ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc atacgctcat cgatgttaga
    52441 aaccagtttc tggaagtacg gtaccggctc cagcgtttct tcattacggt acataccgac
    52501 aacgctgatg cgcgcgctcg gaacgttttc cagcacaccg tccatcatac caagacccgc
    52561 acgcagaatt ggcacaacgg taattttctt acctttgatc tggtcgattt ctaccgggcc
    52621 gttccagcct tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag
    52681 caggctaccc acttcggaag cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg
    52741 catcagtccc agcttgtgtt tgacgagtgg gtgtttgact tccacgatct tcatactctt
    52801 tctcctttga ggggcagcca caaaaaaatc gacggattat acctcctttc ttcaaggcgg
    52861 caatattatt ttcgttgact ttagtcaaaa tgataacagt ttgagataaa gttattttat
    52921 attcaaatgg ttatgaaaga agattattcc atccgaaaac taacctttgc cctggcacaa
    52981 gtcttctttc gccgcgcgcc cggggaaaag acgtgcaaaa aggttgtgta aaacagtctc
    53041 gcaaacgttt gctttccctg ttagaattgc gccgaatttt atttttctac cgcaagtaac
    53101 gcgtggggac ccaagcagtg accgataaaa cctctcttag ctacaaagat gccggtgttg
    53161 atattgacgc gggtaatgct ctggttggaa gaatcaaagg cgtagtgaag aaaacgcgtc
    53221 gtccggaagt gatgggcggt ctgggcggct tcggtgcgct gtgtgcattg ccgcaaaaat
    53281 atcgtgaacc cgtgctggtt tccggcactg acggcgtagg taccaagctg cgtctggcaa
    53341 tggacttaaa acgtcacgac accattggta ttgatctggt cgccatgtgc gttaatgacc
    53401 tggtggtgca aggtgcggaa ccgctgtttt tcctcgacta ttacgcaacc ggaaaactgg
    53461 atgttgatac cgcttcagca gtgatcagcg gcatcgcgga aggttgtctg caatcgggct
    53521 gttctctggt gggtggcgaa acggcagaaa tgccggggat gtatcacggt gaagattacg
    53581 atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga aatcatcgac ggctctaaag
    53641 tcagcgacgg cgatgtgctg attgcactcg gttccagcgg tccgcactcg aacggctatt
    53701 cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc gcaaaccacc gaacttgatg
    53761 gtaagccatt agccgatcat ctgctggcac cgacccgcat ttacgtgaag tcagtgctgg
    53821 agttgattga aaaggtcgat gtgcatgcca ttgcgcacct gaccggcggc ggcttctggg
    53881 aaaacattcc gcgcgtattg ccagataata cccaggcagt gattgatgaa tcttcctggc
    53941 agtggccgga agtgttcaac tggctgcaaa cggcaggtaa cgttgagcgc catgaaatgt
    54001 atcgcacctt caactgcggc gtcgggatga ttatcgccct gcctgctccg gaagtggaca
    54061 aagccctcgc cctgctaaat gccaacggtg aaaacgcgtg gaaaatcggt atcatcaaag
    54121 cctctgattc cgaacaacgc gtggttatcg aataatgaat attgtggtgc ttatttccgg
    54181 caacggaagt aatttacagg caattattga cgcctgtaaa accaacaaaa ttaaaggcac
    54241 cgtacgggca gttttcagca ataaggccga cgcgttcggc cttgaacgcg cccgccaggc
    54301 gggtattgca acgcatacgc tcatcgccag cgcgtttgac agtcgtgaag cctatgaccg
    54361 ggagttgatt catgaaatcg acatgtacgc acccgatgtg gtcgtgctgg ctggttttat
    54421 gcgcattctc agcccggcgt ttgtctccca ctatgccggg cgtttgctga acattcaccc
    54481 ttctctgctg ccgaaatatc ccggattaca cacccatcgt caggcgctgg aaaatggcga
    54541 tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa ctggacggtg gcccggttat
    54601 tttacaggcg aaagtcccgg tatttgctgg tgatacggaa gatgacatca ccgcccgcgt
    54661 gcaaacccag gaacacgcta tttatccact ggtgattagc tggtttgccg atggtcgtct
    54721 gaaaatgcac gaaaacgccg cgtggctgga tggtcaacgt ctgccgccgc agagctacgc
    54781 tgccgacgag taatgccccc gtagttaaag cgccagctct gccgctggcg tttttcaatt
    54841 cacctgtaaa tcgcaagctc cagcagtttt tttccccctt ttctggcata gttggacatc
    54901 tgccaatatt gctcgccata atatccaggc agtgtcccgt gaataaaacg gagtaaaagt
    54961 ggtaatgggt caggaaaagc tatacatcga aaaagagctc agttggttat cgttcaatga
    55021 acgcgtgctt caggaagcgg cggacaaatc taacccgctg attgaaagga tgcgtttcct
    55081 ggggatctat tccaataacc ttgatgagtt ctataaagtc cgcttcgctg aactgaagcg
    55141 acgcatcatt attagcgaag aacaaggctc caacgctcat tcccgccatt tactgggcaa
    55201 aattcagtcc cgggtgctga aagccgatca ggaattcgac ggcctctaca acgagctatt
    55261 gctggagatg gcgcgcaacc agatcttcct gattaatgaa cgccagctct ccgtcaatca
    55321 acaaaactgg ctgcgtcatt attttaagca gtatctgcgt cagcacatta cgccgatttt
    55381 aatcaatcct gacactgact tagtgcagtt cctgaaagat gattacacct atctggcggt
    55441 ggaaattatc cgtagcgata gcatccgtta cgcgctgctg gagatcccat cagataaagt
    55501 gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga cgcaagccga tgattcttct
    55561 ggataacatt ctgcgttact gccttgatga tattttcaaa ggcttctttg attatgacgc
    55621 gctgaatgcc tattcaatga agatgacccg cgatgccgaa tacgatttag tgcatgagat
    55681 ggaagccagc ctgatggagt tgatgtcttc cagtctcaag cagcgtttaa ctgctgagcc
    55741 ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg gttgaagtgt tacgcgaaaa
    55801 actgactatt tcccgctacg actccatcgt ccccggcggt cgttatcata attttaaaga
    55861 ctttattaat tttcccaatg tcggcaaagc caatctggtg aacaaaccac tgccgcgttt
    55921 acgccatatt tggtttgata aagcccagtt ccgcaatggt tttgatgcca ttcgcgaacg
    55981 cgatgtgttg ctctattatc cttatcacac ctttgggcat gtgctggaac tgctgcgtca
    56041 ggcttcgttc gacccgagcg tactggcgat taaaattaac atttaccgcg tggcgaaaga
    56101 ttcacgcatc atcgactcga tgatccacgc cgcacataac ggtaagaaag tcactgtggt
    56161 ggttgagtta caggcgcgtt tcgacgaaga agccaacatt cactgggcga agcgcctgac
    56221 cgaagcaggc gtgcacgtta tcttctctgc gccggggctg aaaattcacg ccaaactgtt
    56281 cctgatttca cgtaaagaaa acggtgaagt ggtccgttac gcacacatcg ggaccgggaa
    56341 ctttaacgaa aaaaccgcgc gtctttatac tgactattcg ttgctgaccg cagatgcgcg
    56401 tatcaccaac gaagtacggc gggtatttaa ctttattgaa aacccatacc gcccggtgac
    56461 atttgattat ttaatggtgt caccgcaaaa ctctcgccgt ctgttatatg aaatggtaga
    56521 ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt atcaccctga agctaaataa
    56581 ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc tccagctccg gcgtaccggt
    56641 taatctgctg gttcgcggaa tgtgttcgct gatccccaat ctggaaggca ttagcgacaa
    56701 cattcgtgcc atcagtattg ttgaccgtta ccttgaacat gaccgggttt atatttttga
    56761 aaatggcggc gataaaaagg tctacctttc ttccgccgac tggatgacgc gcaatattga
    56821 ttatcgtatt gaagtggcga caccgctgct cgatccgcgc ctgaagcagc gggtgctgga
    56881 catcatcgac atattgttca gcgatacggt caaagcacgt tatatcgata aagaactcag
    56941 taatcgctac gttccccgcg gcaatcgccg caaagtacgg gcgcagttgg cgatttacga
    57001 ctacatcaaa tcactcgaac aacctgaata accctatgcc aatacacgat aaatcccctc
    57061 gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag ttttcacatg gtcatagccc
    57121 gtgtggtaga tggtgccatg cagattattg gccgcctgaa acagcgggtg catctggcgg
    57181 acggcctggg gccagataat atgttgagtg aagaggcaat gacgcgcggt ttgaactgtc
    57241 tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc cagcgtctgt atagtcggca
    57301 cccatacgct gcgtcaggcg ctgaacgcca ctgactttct gaaacgcgcg gaaaaggtca
    57361 ttccctaccc gattgaaatt atttccggta atgaagaagc ccgtctgatt tttatgggcg
    57421 tggaacatac ccaaccggaa aaaggtcgca aactggttat tgatattggc ggcggatcta
    57481 cggaactggt gattggtgaa aatttcgaac ctattctcgt tgaaagccgc cggatgggtt
    57541 gtgtcagctt tgcccagctt tattttcctg gcggggtcat caataaagag aattttcagc
    57601 gcgctcgcat ggcggcagca caaaaactgg aaactttaac ctggcaattc cgtattcagg
    57661 gctggaacgt tgcaatgggc gcttccggta ccataaaagc cgcccatgaa gtgttaatgg
    57721 aaatgggcga gaaagacggg ataattaccc cggaacgtct ggaaaaactg gtaaaagaaa
    57781 ttttacgtca ccgtaatttc gcatcgctga gtttaccggg tctttccgaa gagcggaaaa
    57841 cagtcttcgt tcctggactg gcgattttat gtggtgtgtt tgatgcttta gccatccgtg
    57901 aactgcgcct ttctgacggg gcgttacgcg aaggcgtact gtatgaaatg gaaggacgtt
    57961 tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct cgccaaccag tatcacatag
    58021 acagcgaaca ggcccgacga gtgctggata ccactatgca aatgtacgaa cagtggcggg
    58081 aacagcaacc gaagctggcg catccgcaac tggaggcgct actgcgatgg gccgccatgc
    58141 tgcatgaggt cgggttgaat atcaaccaca gcggtttgca tcgccactcc gcttatattc
    58201 tgcaaaacag tgacttgccg ggttttaatc aggaacagca gctgatgatg gcgacactgc
    58261 tgcgctatca ccgtaaagcg attaagctcg acgatctgcc gcgctttacc ttgtttaaga
    58321 agaaacagtt cctgccactg atacagctat tgcgccttgg cgtattacta aacaatcaac
    58381 gtcaggcaac caccacaccg ccaacattga cactgattac tgatgacagt cactggacac
    58441 tgcgtttccc gcatgactgg tttagtcaga atgcgctggt actgcttgat ctggaaaagg
    58501 agcaagaata ctgggaaggc gtggctggct ggcggttgaa aattgaagaa gaaagtaccc
    58561 cagaaatcgc cgcttaatgc tttgcgggcc gacgagaaat gtcggcccgc attattcagg
    58621 cactttcgcg aatgggttcg atttcattca gcgtatcaat taacggttgc ggcttaccaa
    58681 taagataacc ctgcatataa tcgatcccca aagagagcac cgcctcgcgg atctcttcgt
    58741 tttcaacgta ctctgccact acccgcattt tcttcattcg cgccagatgg caaatcgacg
    58801 ccacaatctg ataatccaga ctatttgaca caatattgcg gataaagctg ccgtcaattt
    58861 taagcagatc ggcattcaca tttttaagcc gtgcatagct ggcgtagccg gtgccgaaat
    58921 catcaatcgc aatctggcag cctaattcct gaagatgctg caaggtgatt tgcgcctgct
    58981 taacattggt cagagcatta ctttcggtga cttcaaaaat aagttgccac gcttcaatct
    59041 gatatttagc cagcaactga ctgacttcaa caggaaaacg agcctgacat accgaggttg
    59101 gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc tctgttttca gccataaatt
    59161 gcagcgtatg ctcaatgacc cacatgtcga tactcgacga taaaccaaat tcgtgcgcga
    59221 ccggcaagaa gctatcgggg ccgatcagtt catcattctc acctttcatg cgcagaagaa
    59281 tttcatggta aacatcacca cgcataccgg taatcggctg ggccatcagg aaaaaatggt
    59341 tgtgctccag cgcctgctgt agtcgattca tcatcgcgac tttatctttc aattcgcgtt
    59401 gcaaatacat tgccccgcaa cgctgcatat tttccggggc gttggtcacg atggaaagtt
    59461 cggcgaccgt atttagctct cccagcagca ggtagatatg attcactggc gagcgcacat
    59521 agcagtaact gacgccaatc tgcggttgca tcggcatccc atcccaaaag aaacgaaatt
    59581 gcttgagatg actatccagt gcggtaatgc gctcctggtg cgattctgta ttcagtcgca
    59641 gcgcgagatc gttacccgaa agctgataaa catcttcacc cggttccagc aagggtgaca
    59701 gccagtgaga aagtttttgc ttgt
//
LOCUS       DADMFL010000013        59446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.013, whole genome shotgun sequence.
ACCESSION   DADMFL010000013 DADMFL010000000
VERSION     DADMFL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.013"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            545..1834
                     /locus_tag="JNZ27_000777"
     CDS             545..1834
                     /locus_tag="JNZ27_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY8663218.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1892..3259
                     /gene="sdaB"
                     /locus_tag="JNZ27_000778"
     CDS             1892..3259
                     /gene="sdaB"
                     /locus_tag="JNZ27_000778"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY8663219.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNDNSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3280..4125
                     /gene="xni"
                     /locus_tag="JNZ27_000779"
     CDS             3280..4125
                     /gene="xni"
                     /locus_tag="JNZ27_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY8663220.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="JNZ27_000780"
     CDS             complement(4178..5326)
                     /gene="fucO"
                     /locus_tag="JNZ27_000780"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY8663221.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="JNZ27_000781"
     CDS             complement(5354..6001)
                     /gene="fucA"
                     /locus_tag="JNZ27_000781"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY8663222.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6548..7864
                     /gene="fucP"
                     /locus_tag="JNZ27_000782"
     CDS             6548..7864
                     /gene="fucP"
                     /locus_tag="JNZ27_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY8663223.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7897..9672
                     /gene="fucI"
                     /locus_tag="JNZ27_000783"
     CDS             7897..9672
                     /gene="fucI"
                     /locus_tag="JNZ27_000783"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY8663224.1"
                     /translation="MKKISLPKIGIRPVIDSRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEESKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9781..11200
                     /gene="fucK"
                     /locus_tag="JNZ27_000784"
                     /pseudo
     CDS             9781..11200
                     /gene="fucK"
                     /locus_tag="JNZ27_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11202..11624
                     /gene="fucU"
                     /locus_tag="JNZ27_000785"
     CDS             11202..11624
                     /gene="fucU"
                     /locus_tag="JNZ27_000785"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY8663225.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11682..12413
                     /gene="fucR"
                     /locus_tag="JNZ27_000786"
     CDS             11682..12413
                     /gene="fucR"
                     /locus_tag="JNZ27_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY8663226.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="JNZ27_000787"
     CDS             complement(12457..13557)
                     /gene="rlmM"
                     /locus_tag="JNZ27_000787"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY8663227.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13550..13945)
                     /locus_tag="JNZ27_000788"
     CDS             complement(13550..13945)
                     /locus_tag="JNZ27_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY8663228.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13964..14881)
                     /locus_tag="JNZ27_000789"
     CDS             complement(13964..14881)
                     /locus_tag="JNZ27_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY8663229.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15232..15459)
                     /locus_tag="JNZ27_000790"
     CDS             complement(15232..15459)
                     /locus_tag="JNZ27_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY8663230.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15651..16856
                     /gene="csdA"
                     /locus_tag="JNZ27_000791"
     CDS             15651..16856
                     /gene="csdA"
                     /locus_tag="JNZ27_000791"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY8663231.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16856..17299
                     /gene="csdE"
                     /locus_tag="JNZ27_000792"
     CDS             16856..17299
                     /gene="csdE"
                     /locus_tag="JNZ27_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY8663232.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="JNZ27_000793"
     CDS             complement(17350..18156)
                     /gene="tcdA"
                     /locus_tag="JNZ27_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY8663233.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="JNZ27_000794"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="JNZ27_000794"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY8663234.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19701..19777
                     /locus_tag="JNZ27_000795"
     tRNA            19701..19777
                     /locus_tag="JNZ27_000795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19735..19737,aa:Met,seq:cat)
     gene            19811..19887
                     /locus_tag="JNZ27_000796"
     tRNA            19811..19887
                     /locus_tag="JNZ27_000796"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19845..19847,aa:Met,seq:cat)
     gene            complement(19962..21215)
                     /locus_tag="JNZ27_000797"
     CDS             complement(19962..21215)
                     /locus_tag="JNZ27_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY8663235.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21447..22778
                     /gene="argA"
                     /locus_tag="JNZ27_000798"
     CDS             21447..22778
                     /gene="argA"
                     /locus_tag="JNZ27_000798"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY8663236.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22840..24666)
                     /gene="recD"
                     /locus_tag="JNZ27_000799"
     CDS             complement(22840..24666)
                     /gene="recD"
                     /locus_tag="JNZ27_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY8663237.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24666..28208)
                     /gene="recB"
                     /locus_tag="JNZ27_000800"
     CDS             complement(24666..28208)
                     /gene="recB"
                     /locus_tag="JNZ27_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY8663238.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIGSQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28201..31089)
                     /gene="ptrA"
                     /locus_tag="JNZ27_000801"
     CDS             complement(28201..31089)
                     /gene="ptrA"
                     /locus_tag="JNZ27_000801"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY8663239.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31265..34633)
                     /gene="recC"
                     /locus_tag="JNZ27_000802"
     CDS             complement(31265..34633)
                     /gene="recC"
                     /locus_tag="JNZ27_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY8663240.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNPS"
     gene            complement(34646..34969)
                     /locus_tag="JNZ27_000803"
     CDS             complement(34646..34969)
                     /locus_tag="JNZ27_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY8663241.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34954..35361)
                     /locus_tag="JNZ27_000804"
     CDS             complement(34954..35361)
                     /locus_tag="JNZ27_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY8663242.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35358..35921)
                     /locus_tag="JNZ27_000805"
     CDS             complement(35358..35921)
                     /locus_tag="JNZ27_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY8663243.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35912..36382)
                     /locus_tag="JNZ27_000806"
                     /pseudo
     CDS             complement(35912..36382)
                     /locus_tag="JNZ27_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36566..37360)
                     /gene="thyA"
                     /locus_tag="JNZ27_000807"
     CDS             complement(36566..37360)
                     /gene="thyA"
                     /locus_tag="JNZ27_000807"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY8663244.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37367..38242)
                     /gene="lgt"
                     /locus_tag="JNZ27_000808"
     CDS             complement(37367..38242)
                     /gene="lgt"
                     /locus_tag="JNZ27_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY8663245.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALRWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38393..40639)
                     /gene="ptsP"
                     /locus_tag="JNZ27_000809"
     CDS             complement(38393..40639)
                     /gene="ptsP"
                     /locus_tag="JNZ27_000809"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8663246.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40652..41182)
                     /gene="rppH"
                     /locus_tag="JNZ27_000810"
     CDS             complement(40652..41182)
                     /gene="rppH"
                     /locus_tag="JNZ27_000810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY8663247.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLRRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     LLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41867..42556
                     /gene="mutH"
                     /locus_tag="JNZ27_000811"
     CDS             41867..42556
                     /gene="mutH"
                     /locus_tag="JNZ27_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY8663248.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42625..43338
                     /locus_tag="JNZ27_000812"
     CDS             42625..43338
                     /locus_tag="JNZ27_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8663249.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43476..43694
                     /gene="ygdR"
                     /locus_tag="JNZ27_000813"
     CDS             43476..43694
                     /gene="ygdR"
                     /locus_tag="JNZ27_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY8663250.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43802..44842
                     /locus_tag="JNZ27_000814"
     CDS             43802..44842
                     /locus_tag="JNZ27_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY8663251.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44875..46068)
                     /gene="lplT"
                     /locus_tag="JNZ27_000815"
     CDS             complement(44875..46068)
                     /gene="lplT"
                     /locus_tag="JNZ27_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY8663252.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(46061..48220)
                     /gene="aas"
                     /locus_tag="JNZ27_000816"
     CDS             complement(46061..48220)
                     /gene="aas"
                     /locus_tag="JNZ27_000816"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY8663253.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVLLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48609..49640
                     /gene="galR"
                     /locus_tag="JNZ27_000817"
     CDS             48609..49640
                     /gene="galR"
                     /locus_tag="JNZ27_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY8663254.1"
                     /translation="MPTIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49647..50909)
                     /gene="lysA"
                     /locus_tag="JNZ27_000818"
     CDS             complement(49647..50909)
                     /gene="lysA"
                     /locus_tag="JNZ27_000818"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY8663255.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51031..51966
                     /gene="lysR"
                     /locus_tag="JNZ27_000819"
     CDS             51031..51966
                     /gene="lysR"
                     /locus_tag="JNZ27_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY8663256.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51953..52645)
                     /gene="ygeA"
                     /locus_tag="JNZ27_000820"
     CDS             complement(51953..52645)
                     /gene="ygeA"
                     /locus_tag="JNZ27_000820"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8663257.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52774..54187)
                     /locus_tag="JNZ27_000821"
                     /pseudo
     CDS             complement(52774..54187)
                     /locus_tag="JNZ27_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(54502..55263)
                     /gene="kduD"
                     /locus_tag="JNZ27_000822"
     CDS             complement(54502..55263)
                     /gene="kduD"
                     /locus_tag="JNZ27_000822"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY8663258.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55293..56129)
                     /gene="kduI"
                     /locus_tag="JNZ27_000823"
     CDS             complement(55293..56129)
                     /gene="kduI"
                     /locus_tag="JNZ27_000823"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY8663259.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(56449..57597)
                     /locus_tag="JNZ27_000824"
     CDS             complement(56449..57597)
                     /locus_tag="JNZ27_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY8663260.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            57852..58010
                     /locus_tag="JNZ27_000825"
     CDS             57852..58010
                     /locus_tag="JNZ27_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter"
                     /protein_id="HAY8663261.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTY"
     gene            58167..59081
                     /locus_tag="JNZ27_000826"
     CDS             58167..59081
                     /locus_tag="JNZ27_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8663262.1"
                     /translation="MAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHAT
                     IRVMGFLVFPLIAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSH
                     TPIISTFAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAK
                     EEGVTILSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQV
                     GVKKSRAFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLI
                     PALKPWRSIGNLITLIVGILCVSVMFFS"
BASE COUNT        14649 a        15976 c        14490 g        14331 t
ORIGIN      
        1 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
       61 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgcc
      121 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
      181 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
      241 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
      301 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt
      361 attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
      421 aaattattac agcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
      481 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
      541 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
      601 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
      661 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
      721 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
      781 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
      841 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
      901 aatcaccaat accgttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg
      961 tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca
     1021 gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct
     1081 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
     1141 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
     1201 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
     1261 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
     1321 gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc
     1381 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
     1441 ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
     1501 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
     1561 aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
     1621 gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
     1681 cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
     1741 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg ggctgattgc
     1801 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
     1861 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
     1921 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
     1981 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
     2041 cggctcgctc tctctgactg gtaaaggcca ccacacagat atctccatta ttatgggcct
     2101 ggcgggtaac ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt
     2161 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
     2221 tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac
     2281 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
     2341 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
     2401 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
     2461 cctgatgatg aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc
     2521 gaacgtctgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
     2581 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
     2641 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
     2701 agtgaacgaa gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg
     2761 cgggattatc ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgataa
     2821 ctcactggct cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc
     2881 gtcgatttct ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc
     2941 ggcagctggt ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga
     3001 aatcgcgatg gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt
     3061 accgtgcatt gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc
     3121 actgcgccgt accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
     3181 aacaggtaaa gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa
     3241 gatcgttgcc tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt
     3301 tctcaaccag ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag
     3361 ggggttatcg tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt
     3421 catgccgttc agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt
     3481 atgcacagcc agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc
     3541 tggcgtcatc agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg
     3601 cacgacgaga tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca
     3661 gccagcggca acgaagccga tgacttagcc gccacactgg cggtcaaagt gacactggcc
     3721 gggcatcagg caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca
     3781 ttacgtattc gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa
     3841 tttggcgttc aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca
     3901 aaggtaccgg gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt
     3961 cagagtctgg aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa
     4021 ttagaaaccc ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat
     4081 ttgcatatcg acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac
     4141 gccgcaaatc gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct
     4201 ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg
     4261 ccgcctgagc cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg
     4321 gaataccgac atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt
     4381 ccaggctcat accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
     4441 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
     4501 gtggagtgtt ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
     4561 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
     4621 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
     4681 gtgcatcggt tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
     4741 catcgacacc cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa
     4801 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt
     4861 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg
     4921 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
     4981 caaactccgg gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac
     5041 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
     5101 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
     5161 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
     5221 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
     5281 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
     5341 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
     5401 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
     5461 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
     5521 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
     5581 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
     5641 taccgccagc cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa
     5701 ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
     5761 aggctgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
     5821 tgccatcaat aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
     5881 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
     5941 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
     6001 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
     6061 ttagccgttt aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt
     6121 atattttgtg acctagctca actaatcaca gtaaataact gcaagttctc tttttataac
     6181 cccgttaaaa atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc
     6241 tcgccagaaa tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt
     6301 tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat
     6361 aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg
     6421 aaaatgaatc catgagttca tttcagacag gcaaatattc accgatatga agcccgaact
     6481 cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga
     6541 ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc
     6601 agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg
     6661 ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct
     6721 gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc
     6781 aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt
     6841 atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac
     6901 cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc
     6961 aaaccctttt gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc
     7021 gcaaacattt aactcgtttg gtgcaattat cgcggttgtc tttgggcaaa gtcttatttt
     7081 gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag
     7141 tgcgtataaa cacagcctgg tattatcggt acagacacct tatatgatca tcgtggctat
     7201 cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa
     7261 tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg
     7321 tcactggcgc tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg
     7381 gagctatttg attcgctacg ctgtagaaga aattccaggt atgactgcag gctttgccgc
     7441 taactattta accggaacca tggtgtgctt ctttattggt cgtatcaccg gtacctggct
     7501 catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact
     7561 gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag
     7621 cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca
     7681 ggacaccaaa tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac
     7741 tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccacagctg aactgatccc
     7801 cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa
     7861 ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa
     7921 attggtatcc gcccggttat tgacagtcgt cgcatgggtg ttcgtgagtc gcttgaagaa
     7981 caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatacc
     8041 tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc
     8101 gctgcttgcg aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct
     8161 tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg
     8221 ggctttaacg gcactgaacg ccccggcgct gtttacctgg cagcggctct ggcagctcac
     8281 agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac
     8341 acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc
     8401 gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc
     8461 ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg
     8521 gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa
     8581 atggcactgg cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag
     8641 tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg
     8701 tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa
     8761 tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa
     8821 tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg
     8881 cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg
     8941 ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca
     9001 gaagcaattg agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc
     9061 catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa
     9121 ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc
     9181 gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc
     9241 cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc
     9301 ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat
     9361 gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc
     9421 actggtaaag ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac
     9481 ggagttctga ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt
     9541 atcccggtat gtatgcacaa cgttgaagag tccaaagtgt atcgtccttc tgcctgggct
     9601 gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg
     9661 tacaagcgtt aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg
     9721 aacaagcaag agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt
     9781 atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg
     9841 gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg
     9901 atggaaaaca acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga
     9961 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    10021 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    10081 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc
    10141 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    10201 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat
    10261 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatagccgg
    10321 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    10381 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    10441 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    10501 tcacgatacc cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc
    10561 ctccggtaca tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag
    10621 tcagtacgcc ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat
    10681 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    10741 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    10801 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    10861 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    10921 tctacagatg ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg
    10981 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    11041 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    11101 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    11161 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    11221 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    11281 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    11341 gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg
    11401 ccccgccgct ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac
    11461 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    11521 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    11581 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc
    11641 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    11701 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    11761 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    11821 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    11881 ctccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    11941 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    12001 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    12061 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    12121 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    12181 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    12241 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    12301 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggtatctg gatgaggtaa
    12361 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    12421 ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    12481 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    12541 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    12601 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    12661 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    12721 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    12781 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    12841 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    12901 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    12961 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    13021 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    13081 atagcagcat cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    13141 gttcgccagc accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca
    13201 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    13261 cttctctact acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa
    13321 atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    13381 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    13441 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    13501 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    13561 ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga
    13621 agctcacgcc gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc
    13681 aataaaggct gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    13741 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    13801 acgcctggta ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac
    13861 ttaacacatg cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga
    13921 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    13981 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    14041 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca
    14101 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    14161 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    14221 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    14281 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    14341 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    14401 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    14461 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    14521 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    14581 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    14641 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    14701 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    14761 tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta
    14821 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    14881 tggcttttaa acttcgatgt taaatgaatt taacaattag atcacactgt gtaacctatt
    14941 agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat
    15001 agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac
    15061 ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc
    15121 atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat
    15181 taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc
    15241 cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc
    15301 tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag agacgatggt
    15361 acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt
    15421 cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaagcgatt
    15481 aataaatttt aatagtattg attaacaggc taaaattaac gcctaacact attcagcata
    15541 tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc actctccttg
    15601 tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt
    15661 ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg
    15721 acagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca
    15781 gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc
    15841 gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta
    15901 tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc
    15961 cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg
    16021 tcccctggct gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg
    16081 cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt agtcggattc
    16141 tggcgttggg tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta
    16201 cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg gcggtgcatt
    16261 tccccgcgga tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt
    16321 atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc
    16381 cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat
    16441 ctgcgccgtg gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg
    16501 cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct
    16561 tagcaacgct tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc
    16621 aggattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc
    16681 tactggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg
    16741 cagaattagg cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg
    16801 atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac
    16861 aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa
    16921 taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa
    16981 acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga
    17041 aaaccgcgtc tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcga
    17101 cagcgaaggg cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa
    17161 aaccgccgcc gaattacagg cacagtcgcc gctggcattg tttgatgagc tgggattacg
    17221 tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc
    17281 tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga
    17341 cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac
    17401 cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc
    17461 catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg
    17521 gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt
    17581 cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat
    17641 cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc
    17701 ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg
    17761 tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt
    17821 atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat
    17881 gcgctccgcc atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg
    17941 ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc
    18001 cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat
    18061 atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa
    18121 acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg
    18181 aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat
    18241 aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca
    18301 tccggtatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcaccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgttttgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataagc tttagtggtt tgtttttgtt
    19621 caaaatcatg ctaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga
    19681 gtatagtgcg catccacgga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac
    19741 ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatc aaatttgatg aagtaaaagc
    19801 agtacggtga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg
    19861 tcggttcaaa tccggccccc gcaaccaatt attgaacacc ctaacgggtg tttttttgtt
    19921 tctgatctcc cataaaaaaa gcgccattca gcgccttttt atcatcccct tctcgccagc
    19981 gttgccccat cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc
    20041 tggaaagtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg
    20101 accagaatgg agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga
    20161 tttttatgca gcttgttgat ttttccgagc ttattcagta ccgctttacc aaacttaagg
    20221 ctgtcagcaa tagtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca
    20281 ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt
    20341 gccgcagtac tggttgcgcc tttggttgag agcgcaaaca ccgaggaacc gctcggctga
    20401 cgactggtaa aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct
    20461 tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc
    20521 atattgccct ctttctcgat cagagagcgc aaacggcgag ctatttgcaa taccacatct
    20581 ttttcgcgcg ttttgtattt ccccaccgca ccggaatctt cgccaccgtg gccagggtca
    20641 agcataatga caatcggacg atcgcgccct gctttacccg gttgtggacc actttgcgct
    20701 ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg
    20761 tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg
    20821 gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatg
    20881 cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca
    20941 cgaatttgtg ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc
    21001 accacgcgtt cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct
    21061 accgtcacgc gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctaacc
    21121 gcagccaggc tgacctgact tacgctcaat agccacatag cgcccgcgcc ttgcagtaaa
    21181 cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta
    21241 agctgtgtaa caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct
    21301 gtcatctaat aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg
    21361 ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataattt
    21421 caaataatca tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg
    21481 gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc
    21541 tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt
    21601 tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa
    21661 atctggctgc gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca
    21721 aaacactgga actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc
    21781 tgtcaatgag tctcaataac acgccgctgc agggcgcgca tatcaacgtc gttagtggca
    21841 attttattat tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc
    21901 gtatccggcg gattgatgaa gacgcgatcc atcgtcaact ggaaagcggt gcaatagtgc
    21961 taatggggcc ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga
    22021 ttgccactca actagccatc aaactgaaag ctgaaaagat gattggtttt tgctcttccc
    22081 agggcgttac taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag
    22141 cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc
    22201 gtggcgcagt gaaagcctgc cgcagcggcg tgcgccgctg tcatttaatc agttatcagg
    22261 aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga
    22321 tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt
    22381 tgattcgccc gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga
    22441 tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc
    22501 tctatccgtt cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt
    22561 accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga
    22621 gcggcttaag caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg
    22681 gatttacccc ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc
    22741 agcgtaaatc caaagtgttg atggcggatt tagggtaagc gatgaaaatc accggatgcg
    22801 gcaggcgtaa cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa
    22861 cgccgccaga ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc
    22921 atcggcatac agcgacagac gacgacgcgc gcgggtcacc gcggtgtata ccagctctcg
    22981 cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc
    23041 ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg
    23101 cacagactta atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc
    23161 cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat
    23221 caccggtcgg ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg
    23281 cataaactgc tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc
    23341 gcacaaaagc tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg
    23401 cagcagatcc agataacgtc cgtaacccgc aagagcttcc tcaagcatcg caatataatc
    23461 ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt
    23521 cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct
    23581 gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt
    23641 gccggttcct gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc
    23701 ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga
    23761 ggccagttga tcacgatcgc cgagaaagat cactcgcgca tgatctggca aggcgtcgat
    23821 cagtctcgac atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag
    23881 atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa
    23941 tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa
    24001 ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc
    24061 agccagacgg atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt
    24121 cgccacggtg gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt
    24181 cagcgccact gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt
    24241 gtccagggtt tgcgccagta gagcttcatc aacctcaatg gtatgattca cctcattgaa
    24301 gaagcgcgct accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca
    24361 gaggatcatc ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc
    24421 ttcccaattt tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgacgc
    24481 ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact
    24541 taacagtgcc gccgcgaggg tgacagcagg atgttcatct cccgccacgg tcagggcaaa
    24601 ttgcacatcc agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa
    24661 tttcattacg cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac
    24721 ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc
    24781 aggaacagat agataacgcc gccaaagtga agatcatagt cgtaatcagc aatgcgatgg
    24841 cgcagataac gatgcagtgc cagggtataa agctgatatt gcagatcata gcggtgtgcc
    24901 tgcatcgctg ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg
    24961 gatttatagt cgagcaggta ataacgccct tcgtggcgga ataccaggtc gataaagcct
    25021 tttaacatgc cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca
    25081 aactggcgaa ttagtgcatc aagctggctg ccgatgagcg gttcgctaat cggcaggtaa
    25141 aactccatct ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccgtttca
    25201 ttgagaggtg cctggaggac agccgtgatc cactcggtca acaccggttc ccactgcgat
    25261 tcaaagccgc cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa
    25321 tcgaggtctt caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga
    25381 tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc
    25441 cgtggcatca gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg
    25501 acacgccagt tatcgccggg caatcgttgt agtgtcctcg cattcagttc tgcagttaaa
    25561 gcatcattaa cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca
    25621 tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt
    25681 tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga
    25741 cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg
    25801 taaagcaaac gcagatcttc cgccagacgt tcgacctccg cgaggtcgac gctttctggc
    25861 gcagcattaa gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc
    25921 tggacgcgga aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat
    25981 ttatggatcg tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg
    26041 gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca
    26101 ctttccagct gcgttccggc ttcttgaagc agttcgctga tatgcaagat atcggtaaga
    26161 cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc
    26221 gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct
    26281 tcgactaccg catcccacgc atgttcgtca ttattcagcg tttcaatatc cagcgcattc
    26341 agccccatca ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc gggcgtcatc
    26401 accgcctgca acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg
    26461 ttcgaaaggt aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc
    26521 tcctggcggc tgcgcaccag cacgctgatg tccgaagcac gcaccggacg cgcgtcgtcg
    26581 tcgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca
    26641 gcacatacct gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc
    26701 agccacattt tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt
    26761 ttcccggctg atttcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc
    26821 tggctgaaaa gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct
    26881 aaagtgtagt gggcgtgaac ttcactacgc gccttcatat aagtgaagat atccgcaccc
    26941 cggaatgcat atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga
    27001 tggtgccaga tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg
    27061 atcattgcca ccgggaatcg cgtacggatc gccgctgcca acacctcgcc gctttcgcta
    27121 cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt
    27181 ttttcacgcg ctactgtttc gcggatctca gccaatgctc gggtgatcac cagatcgcgg
    27241 atcgacaatg gttctgcaag cagttgatcg atcacctcaa acagtggatg tcgcggggtt
    27301 tcccccccgg ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc
    27361 ggcaactgat aactgtttgt ctcttcttct gcccaagcgc tgatcttttc gatccattta
    27421 gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc
    27481 agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc
    27541 gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcgcttcg
    27601 ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag
    27661 acgacctggg ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag
    27721 gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat
    27781 tcaaaggcat tcaggttgag catgcgctgg caaaagccgt gaatagtaaa gactgccgct
    27841 tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc
    27901 tcttccagca ggcgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc
    27961 aactcgtgga tattgctacg gatacgaccg cgcaattctg ccgtggcagc ctcggtaaag
    28021 gtgaccacca gcagttcttc aacgatcagc gggcggggaa aggcggcgga accgcctagt
    28081 ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag
    28141 gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca
    28201 tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga cattctccca
    28261 cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg
    28321 cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt
    28381 ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt
    28441 gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg
    28501 cagcatctgg gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg
    28561 caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg
    28621 tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa
    28681 cgcaaacacg gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg
    28741 tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg
    28801 tacaaatacc gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga
    28861 ttgtttttta tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa
    28921 ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt tgccgataac
    28981 cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa
    29041 cgtaatggag ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag
    29101 catctgcgcg ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat
    29161 ctggttatac caggacttcg cctgctcaag ctgctcttcc gtagcggtat agctaaagta
    29221 cccctcgagc aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaac
    29281 cataaggccg ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct
    29341 taactgatca agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg
    29401 ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcagctt tgggctcgct
    29461 ggcaaaataa cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc
    29521 tggatggtcg tatttcttct ctgacttaat cagcgagaaa tcatcaggaa tataagggtt
    29581 aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcgg cgaaagtttg
    29641 tgcgctgatt ttatcgacct gatacggcgc atcgacaaag taagccgttt tgttgtgcgg
    29701 ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc
    29761 ctttactgct ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac
    29821 aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgatcga
    29881 cggataacgg aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat
    29941 gcctttttca cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt
    30001 agccaggcct ttatcagtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac
    30061 gataggatcg gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcaga
    30121 aagtgtacct gggctgcgat tgccaatcag ataggtaatc aattcatcgg ttttactacg
    30181 gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc aacactttac gcggcagcgc
    30241 agggacgtaa tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc
    30301 cgtttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa
    30361 ctccggcagc ggtttattac tgtaaataac cgccttcatc aaattggcgg aatagtactt
    30421 ctcgtggaaa tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc
    30481 gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac
    30541 ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcactgcatt
    30601 acgctcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcat cggccaggcg
    30661 gtctaccgca ccgggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg
    30721 cgcagtgctg gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc
    30781 ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg
    30841 gtacgcctcg ggatcttcca gcgacccaac gggcactacc agcgccgaga gcgatttaac
    30901 tgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata
    30961 ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc
    31021 tgcctgactt aatggtgccc agagggcaac aaacaacaat aatgctttga accaggtgct
    31081 gcggggcatt cacggacctc ataatcaact ttattttctg gacagattca acaggttaac
    31141 gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc
    31201 atcagtccgt gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca
    31261 acactcatga cggattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga
    31321 tggcctccat tgtctctggt gttaattgcc gccagagccg ttgataccag atatcatcac
    31381 cttcgccacg caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt
    31441 gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc
    31501 acgcgccgcc actttcaggt aacaccagca atggcgcgga cattccttca cgatacccct
    31561 caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact
    31621 caccgtcttt gcgtagaaaa aggcgacttt cgccattacc accgctggca cagtagacaa
    31681 ggtgttccag ccaaagttgc attccctgcg ccacacttag taaagaggga cgccagcgca
    31741 acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga
    31801 gatcaatttc catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct
    31861 gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc
    31921 ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca
    31981 ataactgctg attgatttga taacggctaa gtccttccag aataaatggc tcggtgtctg
    32041 ggatttcgct gtcttcagta cggaagttca cctgcaaacg catctggaag aacgcccgca
    32101 ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg
    32161 gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt
    32221 cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac ggcatccggg
    32281 tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt
    32341 catcgcccgg tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg
    32401 acgggaaacg ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt
    32461 gctgcgcgga aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt
    32521 cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat
    32581 aaacgccgtc gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca
    32641 tcagagtaca aatattaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca
    32701 gacgctgtgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac
    32761 ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg
    32821 tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt
    32881 ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag
    32941 ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt
    33001 gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc
    33061 gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc
    33121 cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga
    33181 tgtcaaaccg cgccgccagc accggcacat ccagcagcgc cagcacatcc tctgacacaa
    33241 agcgactgtc aggcagtgat aacaggctga taaacgcttc cagtaccgga tgtgactgcc
    33301 gcgcacgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca
    33361 ctgcctgaat aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg
    33421 gagtaagtgt cggggcttcc tccagcatcg ccagcaagcg atcgtgtaaa acttcaactt
    33481 cacgctgcgg gctatggcaa acgtggaatg tgatactgct atccagtgga tcaagcgggc
    33541 gtttgttatc gctacgggaa aactcttcga tgttcacacc cgcaacggcg cggttttcca
    33601 gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtcaca tcgacaaaag
    33661 cgtccagctc ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct
    33721 tcccccatga ggccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct
    33781 gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac
    33841 gctgacgagt cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac
    33901 ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct
    33961 ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg
    34021 gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt
    34081 gccagcgcgg ttgtccgagt tcatgggtat attccaccag cgccttccac aacggggctt
    34141 gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc
    34201 agtccggacg atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga
    34261 acagttttcg cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc
    34321 gctccagcaa ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg
    34381 cgctctcttt ggggatttcc ggtaacaccc ggacgaacat atcccagata aagctcgctg
    34441 gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc
    34501 actgcgccat accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc
    34561 gttcgcgttc gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt
    34621 agacccttaa catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct
    34681 cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg
    34741 atgtctgcat tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt
    34801 gctgccaacc atgccgccag agctgttggt actggtttct gctggcgaga ctgttcatta
    34861 atgttcgctg gatacccgat aacgcagtga caatcatcac catcagcacc atcgcaacca
    34921 ttacctccgg caggctaaag ccttgttgat tcttcaggga agctgacata atgccccctc
    34981 tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa acaatttttc catcaatgac
    35041 ttcgcctgtt cgccacaacg aaacgccttc ataaccggca atcaataagg cttcattatc
    35101 tgccagtaaa cgcaaacaaa cctgggcatc ggttccagcg tactgcgagc actgaactgc
    35161 tggctgcgtc tgccaggagt gcatttttcc ccacgccagc gccgactgaa cgatggcctg
    35221 gcggcgcaat gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgctgact
    35281 cattccttgc aatagcaagc tacccaaaac cagcagcatc aggaccagag ccagtgacga
    35341 aacacctttt tcgcggttca caggttgaat cctgtcacgc tatagctggc atccaccacg
    35401 gtttgcggtt cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg
    35461 ctgacatcct gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact
    35521 ttatcccagc ccttaccttc acaggatgtc gcaccgcgta gcgtttccag cacatgctcc
    35581 ttcagacgaa atccaatctg gtcggactct tttaccggtt cgcgatccca gataccgtta
    35641 ctgttcgcat cccactgcac aatgatacag tcaccctgcc cgacaatttc cagcccttcg
    35701 ccggtacagc tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc
    35761 cgcagccaga tttcatcttc cagtgccagc ttacgagtgc tcgttaaact ttcacgctgt
    35821 aacgcaggca gaaagcgtgc agcccccagc aacaatacgc tactgatcgc catagcaatc
    35881 aacacttcca gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct
    35941 gataaagccg gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt
    36001 tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg
    36061 tcaggtcgct catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat
    36121 gacaggtatt ggccccagca gcggaactca caaggcacca taacgtcccc tccctgataa
    36181 cgctgatact gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt
    36241 cccgcgccta gctggcggtt tgccaaagcc gttgcgactg ctgccagtat tgccagccat
    36301 agagtccact tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg
    36361 tataaccacg ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc
    36421 cagtttctat ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgcaaa
    36481 atttcgggaa ggcgtctcga agaatttaac ggagggtaaa aaaaccgacg cacactggcg
    36541 tcggctctgg cagtatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    36601 gatcgtagcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    36661 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    36721 ccatatggtt gctgtacagg tgcgtatcgc caccggtcca gacaaaatca cccacttcca
    36781 gatcgcactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttgaacggca
    36841 ggccgaggaa gacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    36901 catagaactg gaagaatgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    36961 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    37021 tagtaatctg gtcaatatga cgaccatctg gcgttggcca ggcgcgccac tgtttaccat
    37081 acactggccc gaggtcgcca ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    37141 cgtgtagata agcaatgtta gtatcgccct gcaggaacca cagcagttcg tggatgatgg
    37201 aacgcaggtg gcaacgtttg gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    37261 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    37321 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    37381 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    37441 tggaaagaat ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg
    37501 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagcctg
    37561 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca
    37621 caccttccag cagcagttcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt
    37681 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    37741 ctgggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    37801 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    37861 agacctggaa gaaggaacgt ttagtacggc gggcgaagat aatcatcacc acgataacgc
    37921 caatcaggcc gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat
    37981 cggccataaa ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga
    38041 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccactgcccg
    38101 gacgattcgc ccgacgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    38161 ggccgtacca gcgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    38221 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    38281 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    38341 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    38401 ctccgcgaat cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatgacgaa
    38461 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcaaaat
    38521 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    38581 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    38641 aacggagatc gataccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    38701 ttgcaggatg aagactgtcg taaatgttcg ccacccgggt attgttgcga tcaacggcca
    38761 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    38821 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    38881 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    38941 cgtcaacttc atcgaggctg gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    39001 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    39061 tgcgaatacc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    39121 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    39181 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    39241 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    39301 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    39361 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    39421 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat
    39481 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    39541 ggatccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    39601 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    39661 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    39721 acagcaatcg ctgacctagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    39781 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    39841 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt
    39901 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc tccggcggca aagcgtttgc
    39961 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca
    40021 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    40081 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    40141 gatattgccc aaacagggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    40201 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    40261 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    40321 aacgttcttc ttttatggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    40381 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    40441 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    40501 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    40561 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    40621 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    40681 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    40741 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    40801 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    40861 cgccgcttac cagcagcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    40921 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    40981 ggattcgaac gtctttgcgc cttaatccta cttcttcaaa caattcacgg tacatcgcct
    41041 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    41101 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    41161 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    41221 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    41281 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    41341 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    41401 gagcattctg aataagcccg atatagacat tttaaaatat tcattattag tcaatgcatt
    41461 acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac
    41521 gggttggcga aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc
    41581 actgaaccac taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatga aaacagtagt
    41641 ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat
    41701 tttcagtgac cagatttgga aaacctattg cagtgttgcg caactcgatt accggcagcc
    41761 taaaaagcaa tataaatcag agaattgaac aacacatgtg gaaaaagtta cactgcgaat
    41821 attcggcaca taattgctgt ttgtttttta accaaggcat catgacatgt cccaacctcg
    41881 cccactgctc tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc
    41941 tggttataca ttgggagaac tggcggcact tgccgggctg gttacgccag agaatttaaa
    42001 acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag
    42061 taaacctgag caagattttg ctgctctggg cgtggaactt aaaactatcc ctgtggatag
    42121 tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg
    42181 ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa cgcgtactgt ggataccggt
    42241 tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta ggatcaccgt tgctgtggag
    42301 cccgaatgaa gaggaagacc gacagctacg cgaagactgg gaagaattaa tggatatgat
    42361 tgttctcggt caggttgagc ggatcaccgc tcgtcacggg gaatatttac agataagacc
    42421 gaaagcagcg aatgcaaaag cgcttaccga agccattggt gcccggggcg aacggattct
    42481 gacgctgcca cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca
    42541 ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt
    42601 catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg
    42661 cttgcgctcg gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc
    42721 ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg
    42781 ttggcgggag ccatggttat gcgtctggcg ctgctggcat caatctcttg ggttacgcgc
    42841 ctgacaaatc cgctttttac aatattcagt caggaaattt ctgcccgtga tttgattctg
    42901 cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa
    42961 ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt
    43021 atgctgctgg atattatctt cagcctcgac tcggtgatta ccgctgtggg tctgtcagat
    43081 cacctgttta ttatgatggc agccgtggtg attgccgtag gcgtgatgat gttcgccgcg
    43141 cgctcgattg gtgattttgt cgaacgccat ccttcggtaa aaatgctggc gctctctttc
    43201 ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt
    43261 tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaactt gattcgcaac
    43321 aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactccttt
    43381 taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcgatt tataaacaga
    43441 ctattatcat aggtaggcca aatgaggata accggatgaa aaaatgggca gtaataattt
    43501 ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca
    43561 aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg
    43621 tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa
    43681 ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc
    43741 ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc
    43801 tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac
    43861 gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt
    43921 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
    43981 aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
    44041 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg
    44101 cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
    44161 cctacagact gattacctcg atctttatca ggtgcactgg ccgcagcgcc cgaccaactg
    44221 cttcggcaaa ctcggttata gctggacgga ttctgcgcct gcggtttcgc tgctggatac
    44281 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
    44341 cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg
    44401 tattgtcacc attcagaacc cttacagtct gttaaaccgc agttttgaag taggtctggc
    44461 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac
    44521 gctgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag
    44581 tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat
    44641 cgccagacgt catggccttg atcctgctca gatggcgctc gcgtttgttc gccgtcaacc
    44701 gtttgttgcc agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga
    44761 aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg tacatcaggt
    44821 ttatacttat cctgcaccat aaagattaac gccggttaaa accggcgtta aatattaatg
    44881 acggcgctgc cagatccaca gcgccgttat tgccagcgca aatagcgcac cgaagccaat
    44941 gccaatgggc acgaccggga tgcctaccat taccgccagc gagtaaatgc ccagcatcaa
    45001 caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct
    45061 ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccagcac
    45121 gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg agaaaatcag
    45181 taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac
    45241 cagcttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata
    45301 ggtcggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa
    45361 acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc
    45421 accattgcac catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt
    45481 ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc
    45541 accaccgtag gccagtgcgc atgcggccag ggcgaccagg acatgccagt cagccagcac
    45601 accaccggct acggaaccga gtaaaatcgc cgctatggca gaagcttcca ttaaaccgtt
    45661 agcttttact aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga
    45721 ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca
    45781 gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc
    45841 gaagctatcc gccacctgcc cgacaaacgg cgccaaaaga atgtaagcac ctacaaacac
    45901 catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt
    45961 ggcaaacagt agcgcgttat cgccaaacgc agagagaaac tgcgccacga taaccgcttt
    46021 catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg
    46081 cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca
    46141 tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg
    46201 catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct
    46261 cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa
    46321 gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt
    46381 ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt
    46441 cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac
    46501 ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc
    46561 cgccctcttc gataccaggg accgacaaca ggcgtgcatc cattcctggt agaatacgcc
    46621 ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg
    46681 tcacaccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac
    46741 tttcttgtag tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt
    46801 tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc
    46861 ggtcatacac caagtccggc acaatgcggt aatgcagcgg gcttggataa aggagcactt
    46921 ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg
    46981 gtaatgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga
    47041 cgttcgccag aatgctttta tggctatgga cgacgccttt tggatggcct tcagaaccgg
    47101 aggtaaagag gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc aggcgcggca
    47161 tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt
    47221 ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
    47281 cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata acactggtca
    47341 gcccttttac cccggcagtg tagttcatca ttgcgggaat gcggcgacgc gcgatggccc
    47401 caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
    47461 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
    47521 aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca
    47581 ttgcactcag taaagattcg tacagagttt cacgcgggcg caccgccata cgcgcttcca
    47641 tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat
    47701 ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt aatttgcggg aacaagcgac
    47761 gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag
    47821 gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca
    47881 gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca
    47941 ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga
    48001 taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca
    48061 ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag
    48121 gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat
    48181 acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga
    48241 ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt
    48301 aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag
    48361 cgtaccgaat aatctcacca atcaatacct ctgggatcac cactttagca acctgaagcc
    48421 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
    48481 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgccac
    48541 cccttgaacc aacgggcgtt ttccgtaaca ctgaaacaat gtaagcgttt acccactaag
    48601 gtattttcat gccgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
    48661 tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctg gctgtgcata
    48721 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
    48781 ccactgaaac ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg
    48841 tgaaagcggt cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt
    48901 accacaacga acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg
    48961 cgttggtcgt ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa
    49021 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
    49081 ttgacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
    49141 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
    49201 attacgatgc ccttgctgaa agtggtattc cggccaatga tcggctggtg acatttggcg
    49261 aaccagacga aaacggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt
    49321 tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc gttctcaatg
    49381 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
    49441 cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtaacg atggcgaccc
    49501 aggctgccga actggctttg gcactggcgg ataatcgccc tctcccggaa attactaatg
    49561 tatttagtcc gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc
    49621 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg
    49681 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
    49741 ggacggctat tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc
    49801 agataatcac ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc
    49861 tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggc
    49921 gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac
    49981 attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc
    50041 atttgtttga cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga
    50101 cccggttcaa tttccagttt cacagggtgg cccaaatggc gagcgatttg ctcacgcgcg
    50161 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
    50221 atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gatgacctga
    50281 cgcaccatag caccacacac ctgctccaga tgggcataat caacgccaga accaatgtgc
    50341 atgtgaatgc cgactagctg cagatgatga cgttgtatca cgtccagtgc ggcgggcaga
    50401 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt
    50461 ccgtgaccaa accccggatt aacgcgcagc catacccgat gccctggcga aacctggccc
    50521 agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
    50581 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc
    50641 ggattgtaac ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc
    50701 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
    50761 cgcaccactt caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag
    50821 acccacaccg ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg
    50881 gtatcggtgc tgaacagtga atgtggcata acaaactcca gatgagtgct tttttatgat
    50941 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgaatctat gcaaaaatga
    51001 tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa
    51061 atttttcatg cggtaatgac cgccggaagc ctgactgagg cagcacacct gctacacacc
    51121 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg
    51181 tttgagcgcg tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
    51241 gtgcaacgat cctggtacgg actggatcgc attgtcagcg ccgcagaaag tctgcgcgag
    51301 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
    51361 cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
    51421 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag
    51481 acactccata cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
    51541 gtgttacctc cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag
    51601 ggtgagaatt acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta
    51661 tttactgaac atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
    51721 tgcgcaatgg tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactagat
    51781 tatgcggcaa gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc
    51841 ctgatccgcc ccctgcaccg cccgtcatca gcgctggttc aggcgtttag tgggcattta
    51901 caagcggggt taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca
    51961 gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac
    52021 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
    52081 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
    52141 tccccagaca cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag
    52201 gaataagaca attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
    52261 tggtgtaacg cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc
    52321 cggtggcatc cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca
    52381 ctttatgcat cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa
    52441 gcgccgcctc agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt
    52501 ctatttcatg aaaatcgacg ctatgtagca gcatttgcgc agagtgaagc ccaccaagcc
    52561 gctgtttaat gccttcattt atcagacgat agtaaggaat ggtggattcc cagctcattc
    52621 ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct
    52681 gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa
    52741 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttctc
    52801 gcctgccatc agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag
    52861 ccagaaagta atgcccacaa acgcaatgtt caacgcagtg tagagccaga acgtaccggc
    52921 agcgccaatg ctatcaagca gtgtcaggaa ggccgcgccg ataatcatat tcgacaccca
    52981 gtttgtcgtc gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga
    53041 gcacaggatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
    53101 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
    53161 gcaatagccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg
    53221 tttacgacct gctttatcta ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag
    53281 tcccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
    53341 gatacgcggc gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
    53401 caacataccg agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc
    53461 gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga
    53521 cgtatcgcgc agcatacgca acacttcttc cgcctcaata tgacgcccct tttccgccag
    53581 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc
    53641 aagaacccca acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac
    53701 gccagcacga tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg
    53761 tacttgccat ttcagaaaga tacagaggag cggtgtaaga cgcgatcccg acagcaatgc
    53821 ccagcaccac acgagcggcg attaacatct ctacgctggt cgcaaaagcg gaccctatag
    53881 aaccgagtac aaacaggatg gcccccgcca tcaggctgta tttacgcccc aggcggaacg
    53941 acagccaacc attaaacagc gcaccaattg ctgcgccgag catcatgcta ctaaccaccc
    54001 attcctgcaa acgactggtc agcacaaagt gatcggtaat gaacggcaac gctccggcga
    54061 ttacgccgat atcaagacca aataacaatc ctgcgaccgc agcagctacc gaaacaaaca
    54121 tattcatacg ccgcgtatca cgcaaagaac gtggcgttaa agcagattcc gtattgatag
    54181 taaccatttt ttcctgccag cagagagtaa gacattgtga aaaaatacgt gaacaattca
    54241 cacaggtgtc aggtcggaaa cagcataaat atggattaaa ttgctgcgac atgccgttat
    54301 gtgatggata ttccaatttt aaaattaagt tgaattattg agattattat taaccaccta
    54361 attttaaagt agataaaatt cataaagttc attaattgat aattgatatg gattatttca
    54421 taaccatgat atggattatg atgatctaga gatataaaaa accctgccat gcggcagggt
    54481 cataaaagta agaggaatga attaacgcgc cagccaaccg ccatccacag caatggtata
    54541 accatttaca taatctgaag cgctggaggc aagaaacact accggcccca tcaggtcact
    54601 cggcagtccc caacggccag ctggaatgcg atcgagaatt tccgcgctac gttgttcatc
    54661 tgcccgcagt tgttgggtat tgttggtcgc catgtaaccc ggagctatcg cattaacatt
    54721 aatgttgtgt ttagcccatt cgtttgccat caatcgcgtc acacccatca ctccgctttt
    54781 tgatgcggta taagaaggca cacggatccc gccctggaag gagagcattg acgcgatatt
    54841 gataatcttg ccgccattgc cttgcgcgat aaagtgtttc gccgctgcct gagacatgaa
    54901 gaatacgctc ttgatattca ggttcatgac atcgtcccag tctttttcgc tgaactcgag
    54961 agcgtcttcg cggcgaatca atccggcgtt attcaccagg atatcaatat gaccaaattc
    55021 agctaccgcg cgatccagca gtccaggaat gccatcaatc tttcgcagat cggcggtcag
    55081 gcttaaaaaa cgacgcccca gcgccgtgac ctgctcgatg gtttcagtcg gttcaacgat
    55141 gttaatgcca acaatgtcac agcccgcttg cgccagcccc aacgccatcc cctggcccag
    55201 cccagtatca caaccagtga cgaccgcaac tttaccttgg agagaaaatg cacttaaaat
    55261 cataacaaca ccttattcgt ttatgcccac aactagcgca aatctttaac ggccacatgg
    55321 tccatatcat taaagacctg gttttcaccg accatgcccc agataaaggt ataagctttg
    55381 gttccgacgc cggaatggat cgaccagctc ggggagatca ccgcctgctc gttatgcatc
    55441 acaatgtgac gcgtttcttg cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc
    55501 atattgaaat agaaataaac ttccatccgg cgctcgtggg tgtgacacgg catggtgttc
    55561 cacaagttac ctggagccag ctccgtcagc cccatactca attgacaggt ttccagtaca
    55621 tccgggacaa aatatttgtt aatcgtgcga cggttactgg tgaggttatc gcctaacgtg
    55681 actggagata cttcgtccgg tgtgactttt ttggtgggat acgtcgtatg cgcgggtgcg
    55741 caattgtaat aaaacttcgc cggagtggcg gtatcaatac tggcaaagac aacttctttt
    55801 gcacctttac caacatacag ggcgtcgcgg tgaccgattt catagcattg accatcgaca
    55861 gtaatcgtac cggcaccgcc aatattgata acacctaact cgcgacgttc aaggaaatag
    55921 cttacgccga gttgtttacc aacttccccg ccaacggaaa ccgttttagt tatcggcata
    55981 atgccgccaa caataattcg gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca
    56041 aatacctttt caaccaaaaa ttcattacgc agcccttggg tatccagcgt ttttgcgtgc
    56101 gcaccgtgga tgctctgtct tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa
    56161 atagaaaacg atagtgaaac actgtttcgt tttctctata aagggatgat atgcccatgg
    56221 gctagggttt tcaattaaaa ataaaacaat gttttatttt taatttaagg attaaaaaaa
    56281 atgcagtgtc gatcacgaat atgtcatgag aatggaaaaa tgagggagcc cagaaaatgt
    56341 atgtcgactt gccagccatc attattcaac agattaatga cggcagatat ttatcggtaa
    56401 ggaggaaatg cagagctact cgtcacgttc aatggtcaat gccacgccct agcccccgcc
    56461 gatacaaagc gttgccagcc ctttgcgggc attacgtttc accatttcat gaaccagaga
    56521 aaccaggatt cggcaaccgg aagcgcctat cgggtgaccg agtgcgatcg cgccaccatt
    56581 gacattgacc cgacgctcat cccactcaag catcttgcca accgaaagcg cctgtgcagc
    56641 aaacgcttca ttagcctcga taagatcgac atcagccaac tgccagccta cacgctccag
    56701 gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat gccggatcta caccaacgct
    56761 ggcaaatgcg cggatgcggg ccagcacggg taaattcaac gctcgtgctt tggcttcgct
    56821 catcatcatt accgcagctg cgccatcgtt tatggatgat gcattccccg cggtcacaga
    56881 accgagacta tcaaatgaag gatttaaacg ggctaaacct tctgcactgg cgtcagtgcg
    56941 tggctgttca tcggtatcaa caaccaacgt ctgaccgtta ctttggatca ttaccgggac
    57001 gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag
    57061 tgcgtaagca tcctgcaact gacggctgat gccatattcg cgagccagat tttcggcggt
    57121 gacaccaata tgataatcat tgaaggcatc ccacaaccca tcatgcacca gactgtcaac
    57181 caactggcta ttgccaagct gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg
    57241 gctcatgttt tcctggccac cggcgatgac aatatcagcc tcgccacact gtatcgcctg
    57301 agtagccaga tgcagtgctt taagaccgga accgcaaacg tcattaatcg tgattgcaga
    57361 aacgctgtta ggaagaccac ctttaatagc cgattgcctt gccggattct gccctgcccc
    57421 tgcagtcaac acctgaccaa gaattacttc atccaccgca tatgcaggaa cgccggtacg
    57481 ttctattaac gctttcacga ccagactacc aagttccacg gcggaatgac ccgctaacgc
    57541 accacgaaag cagccgatag gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac
    57601 aacctcgcac caattaacag tgcaatagta aatgattgtt aattacagtt atcttaattg
    57661 tttaaaaaaa gtgattttta tcacaaagga aatatgcctg agcagtagtc agagtcataa
    57721 ctggcacgta aggtttgcaa ccactaaccc accaatagat gggtagatag ggcgttaatc
    57781 tcccatactt aacttggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat
    57841 acgttcagat aatgtctaat atttggtcta aagaagaaac tctgtggagt ttcgcgctct
    57901 acggcacagc cgttggtgca ggcacgctat tccttcctat tcagttaggt tcggcagggg
    57961 ctgtggtcct gtttattact gctctggtcg cctggccttt aacatattag ccacataaag
    58021 ccttatgcca gttcatcctc tcatcaaaaa catcagcagg tgaagggata acgggcgcgg
    58081 taacacacta ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct
    58141 tttttgtcgt cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg
    58201 caaagcatat ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa
    58261 ttctgaatct catttttctg atgggacgtc atgccactat tcgggtaatg ggatttttgg
    58321 tattcccatt gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac
    58381 ctgatctatt aacaacccag gtagagttca atcagaatac ccttcaccag atatggatat
    58441 cgattcccgt gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag
    58501 acagacgtga aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg
    58561 cttatctcat catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc
    58621 catcttcgta tattgaagcg gctaaagaag aaggggtcac cattttatcg gcgctttcta
    58681 tgctgccgaa cgccccagca tggttgtcaa tttccgggat tattgtcgca gtagttgcga
    58741 tgtcgaaatc attcctgggt acgtactttg gcgttattga aggtgccaca gaggtcgtca
    58801 aaacaacact acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccac gcactatcaa
    58861 ttatgttggt atcgctgatt accttcattg tttgttgcat taacccgaac gcgatttcga
    58921 tgatttacgc gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgctgt
    58981 caacgtatct catcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga
    59041 tcgtgggtat cctgtgcgta tcggtaatgt tctttagcta atatccgttg tggatggttc
    59101 aaaattgcta caattttgaa ccattccatt tttcataata ttcatttgtt attattccgc
    59161 cctatcactt ctctcgtcat atcctagaat tatcagttag ttaaacacac aaagagatct
    59221 gaacatctta aataagacta tttaagatgc ataacttaga ttcgcaagat atcttctggt
    59281 cattcaaaaa caatttgcga tatagatatt ctcataccat gagatagtta tctggaccac
    59341 gttcgaccat gcaagattaa atcagtgaaa cataatcata tttaataacg agaaatgcat
    59401 ttttaaatat cttaatcaga attatagcaa atacagatta atcgca
//
LOCUS       DADMFL010000014        57754 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.014, whole genome shotgun sequence.
ACCESSION   DADMFL010000014 DADMFL010000000
VERSION     DADMFL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.014"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(24..278)
                     /locus_tag="JNZ27_000827"
     CDS             complement(24..278)
                     /locus_tag="JNZ27_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY8663263.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JNZ27_000828"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JNZ27_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY8663264.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JNZ27_000829"
     CDS             1228..2694
                     /locus_tag="JNZ27_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY8663265.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDNAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JNZ27_000830"
     CDS             2812..3798
                     /locus_tag="JNZ27_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY8663266.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JNZ27_000831"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JNZ27_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY8663267.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JNZ27_000832"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JNZ27_000832"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY8663268.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JNZ27_000833"
     CDS             5709..7265
                     /locus_tag="JNZ27_000833"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY8663269.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JNZ27_000834"
     CDS             7347..9161
                     /locus_tag="JNZ27_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8663270.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JNZ27_000835"
     CDS             9162..10256
                     /locus_tag="JNZ27_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY8663271.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JNZ27_000836"
     CDS             10256..11281
                     /locus_tag="JNZ27_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8663272.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JNZ27_000837"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JNZ27_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY8663273.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGIRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARSEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JNZ27_000838"
     CDS             complement(12876..13220)
                     /locus_tag="JNZ27_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663274.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNZ27_000839"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNZ27_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY8663275.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNZ27_000840"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNZ27_000840"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY8663276.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JNZ27_000841"
     CDS             15616..17376
                     /locus_tag="JNZ27_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY8663277.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGAPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JNZ27_000842"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JNZ27_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY8663278.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNZ27_000843"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNZ27_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY8663279.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JNZ27_000844"
     CDS             19113..19340
                     /locus_tag="JNZ27_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY8663280.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JNZ27_000845"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JNZ27_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY8663281.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JNZ27_000846"
     tRNA            21195..21271
                     /locus_tag="JNZ27_000846"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNZ27_000847"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNZ27_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY8663282.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JNZ27_000848"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JNZ27_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY8663283.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGVEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JNZ27_000849"
     CDS             complement(25143..26195)
                     /locus_tag="JNZ27_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY8663284.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNZ27_000850"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNZ27_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY8663285.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JNZ27_000851"
     CDS             complement(26746..28689)
                     /locus_tag="JNZ27_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8663286.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNZ27_000852"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNZ27_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY8663287.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPDVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNZ27_000853"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNZ27_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY8663288.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNZ27_000854"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNZ27_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY8663289.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNZ27_000855"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNZ27_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY8663290.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKCGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNZ27_000856"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNZ27_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY8663291.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNZ27_000857"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNZ27_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY8663292.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNZ27_000858"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNZ27_000858"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY8663293.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNZ27_000859"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNZ27_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY8663294.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNZ27_000860"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNZ27_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY8663295.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNZ27_000861"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNZ27_000861"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY8663296.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNZ27_000862"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNZ27_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY8663297.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNZ27_000863"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNZ27_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY8663298.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="JNZ27_000864"
     CDS             37391..37555
                     /locus_tag="JNZ27_000864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663299.1"
                     /translation="MHTPIGVKPAAGSKEWREAWQKRAFAHISNVYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="JNZ27_000865"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="JNZ27_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY8663300.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39359)
                     /locus_tag="JNZ27_000866"
     CDS             complement(38328..39359)
                     /locus_tag="JNZ27_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY8663301.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVSAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39593..39952)
                     /locus_tag="JNZ27_000867"
                     /pseudo
     CDS             complement(39593..39952)
                     /locus_tag="JNZ27_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNZ27_000868"
     CDS             complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNZ27_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY8663302.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNZ27_000869"
                     /pseudo
     CDS             complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNZ27_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNZ27_000870"
     CDS             complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNZ27_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY8663303.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHVLRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNZ27_000871"
     CDS             complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNZ27_000871"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY8663304.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNZ27_000872"
     CDS             complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNZ27_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8663305.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46647..49319
                     /gene="rcsD"
                     /locus_tag="JNZ27_000873"
     CDS             46647..49319
                     /gene="rcsD"
                     /locus_tag="JNZ27_000873"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY8663306.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDIRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49336..49986
                     /gene="rcsB"
                     /locus_tag="JNZ27_000874"
     CDS             49336..49986
                     /gene="rcsB"
                     /locus_tag="JNZ27_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY8663307.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNZ27_000875"
     CDS             complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNZ27_000875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY8663308.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53310..54086)
                     /locus_tag="JNZ27_000876"
     CDS             complement(53310..54086)
                     /locus_tag="JNZ27_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY8663309.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54091..55380)
                     /locus_tag="JNZ27_000877"
                     /pseudo
     CDS             complement(54091..55380)
                     /locus_tag="JNZ27_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
     gene            complement(55388..56110)
                     /locus_tag="JNZ27_000878"
     CDS             complement(55388..56110)
                     /locus_tag="JNZ27_000878"
                     /inference="COORDINATES: protein motif:HMM:NF036531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663310.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEE
                     EMSFTLQPVTSNEIDSDALYLDEITLTCEQDAVLRYGQVEVPLPPGADVERTTWGISV
                     NKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYV
                     RSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(56107..57666)
                     /locus_tag="JNZ27_000879"
     CDS             complement(56107..57666)
                     /locus_tag="JNZ27_000879"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663311.1"
                     /translation="MTTSELTSSLKGKPVSAQLTVGVVDEMIYALQPEIVPNIGKFFY
                     PLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPS
                     LTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVY
                     RVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLS
                     AELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETP
                     QEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRL
                     MQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQA
                     DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILD
                     TPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGG
                     DATQTAAILMV"
BASE COUNT        14117 a        15571 c        14187 g        13879 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccaca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgataatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtacttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat cgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggcaaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtatac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtagcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgcccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcaacagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacaacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacaacctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga acaagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 tggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cgtctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcacttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcgaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgca gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat gtttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgca gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcaactgcg gtttcggcgt actatctgct
    38401 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    38461 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    38521 gcctgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagcag attcaacata
    38581 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38641 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38701 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38761 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38821 tatttcacca gctcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38881 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38941 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    39001 accgacatcg gtggtgcgct aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    39061 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    39121 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    39181 aagacaaatt tggtgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    39241 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    39301 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    39361 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    39421 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    39481 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39541 cggtattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39601 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39661 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39721 tgttccagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39781 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
    39841 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39901 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39961 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    40021 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    40081 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    40141 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacgcgaa
    40201 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    40261 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    40321 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    40381 gccgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcggc
    40441 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    40501 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40561 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40621 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40681 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40741 ttgccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40801 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40861 ggaaacgcat gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40921 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40981 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    41041 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    41101 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    41161 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtt cagagtcagc
    41221 tctttgcgcc cttcaagtac agtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgt
    41281 agggtcgcca tatgtttgta cacccgcgcc accagcacaa aaccgcccca gatggtgatc
    41341 gccatccaga tagccgttac cagcaacatt ttgcccgaca gcatccacag atacgccgat
    41401 gaaccgatag tgagaatgat cccctgcccc agttccggca gacgcacaaa agcaatggtg
    41461 atattgcgca catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg
    41521 cgctcgacgt gggtatccag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag
    41581 tgatgcccca aagtggtgag cgccagttgc gatccgagag tgactgccat caacagcagc
    41641 aataatccca gaaactccgg taacaccagc agactggtat ccgccgtttc gataaggcgc
    41701 tgattgataa aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc
    41761 acactgataa atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa
    41821 acccggacaa tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat
    41881 tcttattttt attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcggtg
    41941 gtgagtggat gaaaccccgc tttcaacggt tgaataccgt gataaaacag ccgcgattca
    42001 ccgccccagg ccaccaaatt gaaaaatcgc gggtaagccc agagaaacag aaacaattgg
    42061 cgcgcgcaga tccggttcgt ctttatcctg atgcagcgac agtttcgcgc caggagcgta
    42121 gcggttgata agacaagcat ctggctggaa atctggatag cccgccgccg tagccgcacg
    42181 ttgacataaa tcatgaaagc tttgtggcat ggcgggccac ggtttatttg tttgcggatc
    42241 aatgggcgaa tagagataac cttgccgatg ggtcgtccag cccagatgcc cacagttggt
    42301 catcgccacc gacatggtat atccgcctgg agcgaccatc tggcgaaacg gcgactggct
    42361 ggcaacgtca ttaatatcgc ggattagttg ctccgcagca ttaaaagcaa aacgccgtaa
    42421 aattaccgca ccagccgcca gtggctcttg ccacggttca gcatcggcaa acagatccaa
    42481 cattacctct cctcattttc agcttcgcgg cgcagcagtt gcgctttacg cgacacgccc
    42541 cagcggtaac cggaaagtgt gccatcacca cggaccactc gatgacaggg tattacgata
    42601 gccagcttgt tggcggcaca ggcgctggca acggcccgta ccgctttcgg tttgccgatg
    42661 gcgttagcca gttgctgata actgacggtt tcaccacaag gtatcgtgcg cagtgcctgc
    42721 cagacttgtt gctgaaaagc agtgccgcga atatccagcg gtaacgtcag cggcgtatcg
    42781 cgttgattga ggctggcgat cacttcacgc acatgttgct gaaacgtcag atcggcaggc
    42841 gcgttgtcgg cagcgggaaa catctgctgc aactcgctga ttaatgtcgc gtcatcatcg
    42901 cccagcaata tcgcgcaaat cccccgctcg ctttctgcca ccaggcaacg acccagctca
    42961 caatcagcca gcgcgtaacg caccgccaga ttttcgccac cgtgacggaa ttgtttagcc
    43021 gtcatgccca gcgtttcatc ggctttgcgg tagtaactgc tgctgtcggg gaatccggcg
    43081 ttaagaatag acgtcgtcac gctctcccct ttcgccagcg attcgcgcaa acggcgagcg
    43141 cgccaggcct gttgccaggc tttaggcgtc attccggtag tagctttaaa caaccgatgt
    43201 agatgaaatg gactcatcgc cacctggtcg gctaaggctt ccagcgttac aggcgtttcc
    43261 tgttccagca gtcgacacgc gtgggtgatt ttatccaacc gatgttgccg gggattggct
    43321 ttgtctggct gacaacgttt gcaggggcga aatccggcgg cgagtgcctc gctggcattt
    43381 gcgtagaagg agacattttc ccgcaagaca tgtctggcgc ggcaagacgg acggcaaaag
    43441 atacccgtgg tgcgcacggc gaaaacgaat tcgccgtcgg cattcgggtc gcgggctaag
    43501 acagattgcc agcgttgatc gtcagttaag catgtggcgt ttttcataat cagctccctg
    43561 gttaaggata gcctttagtc tgcccggtca ccatcacgca aaaaccaaca atcttgcgct
    43621 ttaatttttt tcgctgacaa ggaagctttt aaactgtggt gacatccagg ttttaaagct
    43681 atcgccttct ttggtgatca tataaaccgc cagcccttcc cggcgaacaa cctctttggc
    43741 tttctccggc ccgagtacca tcaagccagt atcccaggca tcggcttcca gcgccgtcgg
    43801 agcaatcacc gtcacggata ccagattgtg ttcgatggga cgcccggttt gcggatcgat
    43861 aacatgggaa agacgtttgc cgtccagttc gtaatagtta cggtagctgc cagaggtgct
    43921 aatcccatga ccgttgatat ccaccacagc ctgaaccgcg ttttctttat cggttggttt
    43981 ttgaatcgct acccgccacg gctggccttc accgttcata ccacggctgt tcagcgcgcc
    44041 gcccaccgac accagatagc gggaaatccc ttcctgctcc atcaagcgtg ccaggtgatc
    44101 cgccgcataa ccttcaccga cggtggagag atcgacatat aaatccggca ggtctttttg
    44161 cagatattgc tgatgcgact gattgatgac cgtcaggtgc tgtaagccgg ttttggcttt
    44221 catcgcatcg atctgttcct ggctcggaat ttgaaccggc tgttgttccg ggccaaagcc
    44281 ccacagattc accagcggcc cgacggtaat atccatcgca ccatcggtcc ttgccccaat
    44341 gcgcagcgag gtggtgacga tatcggccat cgcttcactt accggccacg gcgacaaact
    44401 ttgcgagtcg ttaaagcgca tcagcgcgga atctttttta taggtcgaaa gcagctgatc
    44461 gtcggcgtcc agctgggtct gaatcttttc tttaagttcg gcactgcgtt tggcatcaat
    44521 gcccgggatg ctggcacgcc agaaggtacc catggttttg ccttcaagaa cggtaacttc
    44581 agtagcgtgg gttttggcgg gttgtggttt ttgatcgcaa ccaacaaaga agagcgcggc
    44641 aaccagcagt gccacgcggg taaagcttat ttccatacgt gattatcctc atgcgaacgg
    44701 tcgcaagagt acaccaaaaa actgtgtttg tacgctgaaa acaatgaaaa aagggcccgc
    44761 aggccctttg ttcgatatcc atcgagatta gaactggtaa accagaccca gagctacgat
    44821 gttatcagtg ttgatgccag cgtcacgagt gaactggttg tcgtccagca ggttgatttt
    44881 gtagtcaacg taggtggaca tgtttttgtt gaagtagtag gtcgcaccaa catcaacata
    44941 tttcaggata tcttcgtcgt cgtagttacg accattgatg acacccaggt ttttaccttt
    45001 agactgcagg taagctaggg acggacgcag accgaagtcg aactggtact gagcaacagc
    45061 ttcgaagttc tgtgctttgt tcgcccaacc caagttgcct acgcgagttg cgttgtaggt
    45121 ctgggtgtac tgagcagcta ggtagatgtt gttcgcgtcg tatttcagac caccggtgta
    45181 ggtttcagcg cggtcgccat taccgatgta acgctcatca taacggttca gaccgaagtt
    45241 ctggtcatca gtacgtttgg agctggaaac tgcagcaccg ataccgaagc cttcgtaatc
    45301 ataagtaata gaaccgccaa cgccgtcacc gttctgacgc agagcttcac gaccgttgtt
    45361 ggtcatgcct tcaccttccg ggctaccgtt tttgccctga tactgaacag cgaagttcag
    45421 accgtcaacc agaccaaaga agtcggtgct acggtaggtt gcgaagccgt taccacgctg
    45481 ctgcatgaag ttgtcagaac cgtaagtgtc accaccgaat tctggcagta cgtcggtcca
    45541 ggaagttacg tcgtaaacta cgccgtagtt acgaccgtag tcgaaagaac ctacatcctg
    45601 gaatttcaga cctgcgaatg ccacacgggt ccaggagttg ttttcgtttt cagcgctgtt
    45661 gccctggatc tgatattccc actggccgta accggtcagc tggtcagtaa cctgagtttc
    45721 acctttgaag ccaagacgca tgtaggtctg gtcgccatct acagacttgt cgtcagagaa
    45781 atagtgcagg ccgtctactt taccgtacag atctaatttg ttgccgtctt tgttgtaaac
    45841 ttcagcagcg tttgctgctc ctgctaccag cagagctggg accaggaggg acagtacttt
    45901 aactttcatg ttattaaccc tctgttatat gcctttattt gcttttttat gccactgcat
    45961 actgattaac cctcattaat cagtcggcaa gtccattctc cccaaaaatg cagaataatc
    46021 caacacgaat atgatactaa aacttttaag atgtttcatt tatcgctata gatgtttcaa
    46081 aatgtaaatg caagggaact ttttaagatt attgcggaat ggcgaaataa gcacctaaca
    46141 tcaagcaata ataattcaag gttaaaatca ataacttatt cttaagtatt tgacagcact
    46201 gaatgtcaaa acaaaacctt cactcgcaac tagaataact cccgctatca tcattaactt
    46261 tatttattac cgtcattcat ttctgaatgt ctgtttaccc ctatttcaac cggatgcctc
    46321 gcattcggtt tttttaccct tctttacaca cttttcatta ttctgtgcta ccacagaaaa
    46381 actataacgc ttgttaacta tttcacaaat aattaacatc agcataattt ccagcaatct
    46441 ttgtttattt acaattaatt tcgttgggct ttttgtaggt tatttgtaca gcaaaatggc
    46501 gcttgtacat ctatttcccc ccatacagga tgataaatat cacgggagaa tagagaatca
    46561 tcaatcaggt aagagtctgg aatttcacac tgtacccttt atactgccct atcacttcgc
    46621 gaagttttaa caggtcataa acacgaatgc gtcagaaaga gacaacggcc acgacccgct
    46681 tttcactcct accggggagc attacccgct tctttttact gttgatcatt gtgttactgg
    46741 tgacgatggg ggtaatggta caaagtgccg ttaacgcctg gctgaaagat aaaagttacc
    46801 agattgtcga cattacccac gcaatccaaa agcgcgtcga tacctggcgt tacgtgacca
    46861 ggcagatcta cgacaacatt gccgcgacga cctccccctc ctccggcgaa ggtttacaag
    46921 agacgcgcct gaaacaggat gtctactatc tggagaaacc acgccgcaaa acggaagcgt
    46981 taatctttgg ctctcacgac aactcaacgc ttgagatgac tcagcggatg tccacttatc
    47041 tggatacatt gtggggtgca gaaaatgtac cgtggtcgat gtattacctg aatggtcagg
    47101 ataacagtct ggtgctgatc tcaaccctac cccttaaaga tctcacctcc ggatttaaag
    47161 aatcgaccgt cagtgacatt gttgattcac gtcgtgcaga aatgttgcaa caggccaacg
    47221 ccctcgatga acgcgaaagt ttttctaaca tgcgccgcct ggcctggcag aacggtcatt
    47281 actttacctt gcgtaccacg ttcaaccagc cgggacatct ggcaacggtc gtggcttttg
    47341 atctgccgat taatgatttg atcccaccgg gtatgccgct ggacagtttc cgccttgagc
    47401 cagacgcgac ggcaacggga aacaatgata atgagaaaga agggacggat agcgtcagta
    47461 tccactttaa cagtacgaag attgaaatct cctcggcact caactctacc gatatacgcc
    47521 tggtctggca ggttccttat ggcaccttat tgctggatac gttgcaaaac attctgctgc
    47581 cactgctgct gaacatcggt ttgctggcgc tggcgttatt tggctatacc acattccgcc
    47641 atttctccag ccgcagtaca gaaagcgtcc ccagcacggc ggtcaataac gaattgcgca
    47701 ttttacgggc aattaatgaa gagatagtct cactgttgcc gctcggcctg ctggttcacg
    47761 atcaggaatc gaaccgcact gtcataagta acaaaattgc cgatcatttg ctgccgcatc
    47821 tgaatctgca aaacatcacc accatggcgg aacagcatca ggggattatt caggcgacga
    47881 tcaataacga gctgtatgag atccgcatgt tccgcagcca ggtcgcgccg cgcacacaaa
    47941 ttttcattat tcgcgatcag gatcgcgaag tgctggtaaa caagaaactc aagcaggcgc
    48001 agcgtctgta tgagaaaaac cagcaggggc ggatgacctt tatgaaaaac attggcgatg
    48061 cgctgaaaga acccgcacag tccctggcgg agagtgcggc taaactcaac gccccggaaa
    48121 gcaaacaact ggcgaatcag gcagatgtgc tggtgcggct ggtcgaagaa atacagttag
    48181 cgaacatgct tgcggatgat agctggaaaa gtgagacggt gctgttctcc gtgcaggatt
    48241 taattgatga agttgtgcct tcagtgttgc ctgccatcaa gcgtaaaggt ctgcaactgc
    48301 tgattaacaa tcatctgaaa gcacacgata tgcgccgcgg cgatcgcgat gccttacgac
    48361 gtattttgct gctactgatg caatatgccg tgacctcaac gcaattggga aaaatcaccc
    48421 ttgaggttga tcaggatgag tcctctgaag accgcctgac attccgcatt ctggacacgg
    48481 gagaaggcgt aagtattcat gaaatggata atttgcactt cccgtttatc aaccagaccc
    48541 aaaacgatcg ttatggaaag gcggacccgc tggcattctg gctgagcgat caactggcac
    48601 gtaaactggg cggtcattta aacatcaaaa cgcgggatgg gcttggtaca cgctactctg
    48661 tgcatatcaa aatgctcgcc gctgacccgg aagttgaaga ggaagaagag cgtttactgg
    48721 atgatgtctg cgtaatggtg gatgttactt cggcagaaat tcggaatatt gtcactcgcc
    48781 tgttagaaaa ttggggtgca acctgtatca cacccgatga aagattaatt agtcaagatt
    48841 atgatatctt tttaacggat aatccgtcta atcttactgc ctctggcttg cttttaagcg
    48901 atgatgagtc tggcgtacga gaaattgggc ctggtcaatt gtgcgtcaac ttcaatatga
    48961 gcaacgctat gcaggaagcg gtcttacaat taattgaagt gcaactggcg caggaagagg
    49021 tgacagaatc gcctctgggc ggagatgaaa atgcgcaact ccatgccagc ggctattatg
    49081 cgctctttgt agacacagta ccggatgatg ttaagaggct gtatactgaa gcagcaacca
    49141 gtgactttgc tgcgttagcc caaacggctc atcgtcttaa aggcgtattt gccatgctaa
    49201 atctggtacc cggcaagcag ttatgtgaaa cgctggaaca tctgattcgt gagaaggatg
    49261 ttccaagaat agaaaaatac atcagcgaca ttgacagtta tgtcaagagc ttgctgtagc
    49321 aaggtagcct attacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc
    49381 ttgttcggta ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt
    49441 gaagactcta cagcactgat caacaacctg ccgaaactgg atgcgcatgt gttgattacc
    49501 gatctctcca tgcctggcga taagtacggc gatggcatta ccttaatcaa gtacatcaag
    49561 cgccatttcc caagcctgtc gatcattgtt ctgactatga acaacaaccc ggcgattctt
    49621 agtgcggtat tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat
    49681 ctgccgaaag ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct
    49741 cgcctgttgg aaaaaatcag tgctggtggt tacggtgaca agcgtctctc gccaaaagag
    49801 agtgaagttc tgcgcctgtt tgcggaaggc ctgctagtga ccgagatcgc taaaaagctg
    49861 aaccgcagta ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggcgtc
    49921 gagaacgata tcgccctgct gaattatctc tcttctgtga ccttaagtcc ggcagataaa
    49981 gactaatcat ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcaccgat
    50041 tgccggatgc ggcgtaaacg ccttatccgg cctacgattc ccattatttc aacagattac
    50101 attaaagtag gccagataag acgcgtcagt gtcttatctg gcatttgcac tgaatgccgg
    50161 atgcggcgta aacgccttat ccgtcctacg aatcccgcga tttcctgacc ctctcggcat
    50221 ataccgtcag cgtctgtttt atcacatcca gcgttaccgg cttcgagagg cagctgtcca
    50281 taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta
    50341 ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg
    50401 gcatgttgac gtcgctaagc acgatatcaa tatgattctt actaagtaca ttaagcgcat
    50461 cgacgccatc attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca
    50521 gacgtcggtt aatcggatga tcatccacga ccagaatcat catatcgtcg ttatcgctga
    50581 ccgctttgtc cgtcgacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat
    50641 aaatacgcgc caacaatgcc ggtagctcat gcggagcagc cacactgtgt acccactccc
    50701 ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcact accgctctgc
    50761 cctgccattt tttactcact acctcgtcag tgattaacac atcttcggga gtcggttcct
    50821 gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac
    50881 agagcgacgc attgcggacc gccagccagc agcgtttacc actcaaccct tccacgcctt
    50941 ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc
    51001 ccggttctga atctaccgag atatcgccgt ccatcatgct gatcagtttt tcacaaatcg
    51061 ccagacccag accggtcccc tggaagtttc gctgtacgcc cgttccaacc tggaagaagg
    51121 gatcaaacaa gcgcaccact tctttcgccg gaatccccac gccggtatcg cgaacacgga
    51181 tagagagata atcgccatca gcgcgaacat gcaaaactat acagccggta tcggtgaatt
    51241 ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg tcgccattta
    51301 aggccactgg cacatccggt tcaataaagc agtacaagcc taactgcttg cgtaccacca
    51361 gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt
    51421 cgatcttcaa ctgttccgat tcaatcttcg agaaatcgag aatatcgctg ataattttca
    51481 acaacaggct ggaagagttg ttcattgccg tcaccagacg atcgacgcct ttcggtaact
    51541 ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat
    51601 gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca
    51661 tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg
    51721 ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcagattg gtattgttgc
    51781 tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgcgtc agtcgttggc
    51841 ggtcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga
    51901 cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat
    51961 tgaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgtc
    52021 gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg
    52081 cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc gaatacacga
    52141 tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac ccttccgtat
    52201 agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaatttta ctttctggtc
    52261 cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg
    52321 tacccggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa agcgcctgca
    52381 accggttcgc cagataaact ggcgtcaacg cgtaaaaata cccgacgcca gggcgcggac
    52441 cttcgctgat ccagtagagg ttactgccgc tatcatcttg tggtgcattt cgatatttat
    52501 tgatgcgttc atgcaacgct ttcaacgcgg tatcgcgttc cactggcata tcacgcagac
    52561 cgaaattggc catgcagagg ttatcactgc cgattaaaaa tacccggttg agatcgtaag
    52621 ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc
    52681 gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa cagcggttca aacgcaggca
    52741 catccgcctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat
    52801 tttcggcgat gtacttaagc tctttcatca catcagaggt gcgttgaata aagcgctgag
    52861 cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat
    52921 taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc
    52981 tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg
    53041 cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac
    53101 cgcaatgttc tgtcaccatt gcaataatgc gtatcgctta gaaaccattg ccttaaccag
    53161 gaaagccgga gtagatggac agttctcagt acgaaaaata agaaatgacc aatctgccga
    53221 cgcttgttaa tggttatatc ctggcgtcgg cagatcgccc agcagtctta tctgaaaaaa
    53281 gagagagcca ggttgtgtga agggatggat caccagtcaa aacttttcac cagcgtcagc
    53341 tcgccagcat tacgcatcgg tacaataaat gtttcctgtt tctcattgac cgatccttca
    53401 tcggtgatca gcgtcagttg ggcggtcgtc aattccgttt cgctgcgccc accatagtag
    53461 ttgatataca cctgatagcg cccgtgaact ggtgctggca tggcgaaaat ctcgggtccg
    53521 taccccgtcg tgacatccat atccagtgca ccactgtttt tcagcacggt gttaccgtac
    53581 caggcgtgtt cgccatcggg cgtgacaaca tggagatcga gatcggtatt gtccgtatcc
    53641 cacgagagaa ccagccgtaa acgtgcacga atagttcctg tacccggcgt tgagtaaaac
    53701 tgcatttttt gccggctttg cccatccggg ctgatgacct gcacgctgtt gctgccttca
    53761 gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa ttctctgcgg catactggca
    53821 ccgttgacca ccagccggcc ctgctgaact ttacccgccg cagcataatt ctttattttg
    53881 ccgctgatct gcgctgagat attttgatca tctgccatgt tgacggacga cgccgggtaa
    53941 ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg gatgccagcc agagagcggt
    54001 gcgtcaatct cgacttcctg cacaccttca ctgtgagcga caggcgaaag tgccaccagt
    54061 aacaacggaa gaaaaatctt tcgcatagca ttagtccatt aataattgtc gggtcagcgt
    54121 ttcgatatag ttctcatcaa gcccggtggg ataaccatca aaggccaagt gcagatactc
    54181 atgggttaaa tccagccgat cctgaagcgt aaagaagtta cgaataaaca gccgtttctg
    54241 ctgccgatcg gtatagggga aacctgagac taaacgacag accgcaaaca cgtctggttc
    54301 gttgtacccc gtctcgcctt gtaatatacg ccgccactgc ggcatttttt tcgccagcca
    54361 ggctttcgct ttgggtaata actggcaggt tgagcgtgga gctccccagc ggctaaggct
    54421 gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc tccccctgcc ccgcctgggc
    54481 cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga ctgccatgat aatgaaccgg
    54541 atcacctgca tagatgagct cctgcgtcca ggcggtcatc gttcgcgccc ccgtggtcgc
    54601 tggcgaagca gaaacacgct gcgtggcgct gctgccgggg atggtcaggc aatcaccttc
    54661 ccgattggcg ttttgctgta aaaaagtacg tatcgccaca gtcatggctt tcgctgcctc
    54721 aggtggcgta cttttcgcct cgcgatccag aactctggca acgtactctt cacgatcgag
    54781 atgcgactgc aatttcagtt tgcccttttc gctcagtaag gtcgtctcac cgtggctgac
    54841 aaacgtaatg tggttaccat tagtaaacgt cacacgatag cgcccattaa gtacgctggg
    54901 ttttactgcc gtcgtactct tttccgctgt gattttcttc agcggatagc gggcaaacag
    54961 ctccacctct acacattgcc cactggcaac ctgcgtcggc actggcaaaa cccgattaag
    55021 caccgtcgca taacgggtta gtaccgtttt gctggtgcca ctgccagtaa cccacagcgg
    55081 tgtaccatcg gttaaccagc cagcaaaacc accctgacgg atttcctgtt tatcatcggc
    55141 aaaccagctc caggttttca cccgtacccg actaccgagc atggatgaga caccgatttt
    55201 cgcctcgtcc agcaccacat ccagtaagac ttcctgagct ttgttttgcg caggtaacgt
    55261 cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt tctggcttca gcgttgtaag
    55321 agacgctaac cacgcaggtg cctgacgctg ttgccagtat tgcccccaca catctgcgcc
    55381 agttcactta tttcacctgc atcccggtcc attcactccc tgccgcgaca ctttgctgcg
    55441 caggtgcata ggaacgcaca taacgagcag gaggcagaac gaattgccct ttttgcgaga
    55501 agcgcagcaa atggcggaaa gtgaccgttc ccgtcagttc tttcaccggc accatatagg
    55561 ccagttcgcc catttcatta cgcgcttttt ccagcaattg cccctgctgt ttcgcggcgt
    55621 tgggtttatt gactgaaatg ccccatgttg tgcgctcaac gtcggctccc ggcgggagcg
    55681 gtacttccac ctgaccatag cgcagaactg catcctgctc gcaggtaagc gtaatttcat
    55741 cgagatacag cgcatcgctg tcaatctcat tgctggtcac cggctgcaga gtaaagctca
    55801 tctcttcttc accggggata agccgataca actggcgttc aacggtcacc ggaatgttac
    55861 tttgttgagc cgtttttgcc ggttcacgcc agcggacctg cacattttgc ggcgataatt
    55921 cgtcaccaaa agagagaatg tccggcaccc cctgaccaac ccaacgccag tcttcgcccc
    55981 ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc aggcaaaaca actggaggca
    56041 ttgtcgccat atatttcgcc agccagttca tggccagcgc gcgctcaata gtggattgct
    56101 cagcggtcaa accattaaaa tagcggccgt ctgggtagcg tcaccaccgc tattgagcag
    56161 cacaaccgtg tgcgccagcg gctgattgct gtttgccgct ttatcccacg catattgctg
    56221 aacctgtggc atcaccgtgg acttcaactg cgcttttttc agcaacgtca tggttaagac
    56281 gtttgccacc gcatctgcca gtggagattc cggtgtatcg aggatcaggc tgtcattgat
    56341 atcgcggatg tcttcctcag agaaatcttc atctttagtt ccgcgccggg cgatagcttc
    56401 atccccccct ttcaacaacg ttttgcacgg cagattcatc tcctgtgccc acgccagcac
    56461 cagcgcccga tgcaataacg gcatgttatc cgcctgctcg gcataactgt cgagcatatg
    56521 ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg ctggcctgcc aatcggcgta
    56581 ccatgcccat gccgtaagga aggcgtcacc attgccatct tcaccccacc aggtaaagcg
    56641 cgctccgggc cccgccagtt gcatcagccg cagacggtta tcctgaatca tctgacgaat
    56701 gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc caggcaagac tgagcgggat
    56761 cagacggcta ccggtgttga tcaccccacc ccacggttca tcgactaacg catcaagatt
    56821 gttgcggaaa atctcctgcg gcgtttcact actttgtagc cggatattac tcgcctgctc
    56881 gggcaacatc agcgcgttct cgccaccgcc gagcatgaca tttttctgct gttcaacggg
    56941 ccagctgttg tccacaaaag acagttttgt gctaatgctg tcctgcactt gcccattttg
    57001 ttgcagttct gcacttaaca agccagattg ctgaatattc tgcgtcagcg aaatataatt
    57061 cgcccctttg tgcagcgtca gcgtctggcg catctcagcg cctgcaaatt tagtcaccag
    57121 cgccaccggt tcgttatcct gctgactgaa gataaacagt cctgccgccg gtttgtcgcc
    57181 cacgcgatac accgttggca tactccactt catgtagaga tttttttccg aacgcagata
    57241 agcacgcccc tgcccgacca gcccatcgcc gttcatccca cgcgcggtga tacgccagcg
    57301 ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg ccttgtttat cggttgtgag
    57361 tgacggcatc catgccgcgg tatccacctc ttcacgccgt ggacgttcaa gcatttttac
    57421 tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc tcgctggaga gcgcctggtc
    57481 atagctgata aacgacagac tggagctggt acgcacattg ttacgcccca gcggatagaa
    57541 aaatttgccg atattcggca cgatttctgg ttgcagcgcg tagatcattt catcgaccac
    57601 gcctaccgtt agctgcgcag aaacaggttt acctttcagc gacgaggtta attctgaagt
    57661 ggtcaacaaa aactgtccac cgagttagag tttttccagt atcgattttc cgattcgttt
    57721 gggggtaacc caccgttata ttcgtgcggt ctta
//
LOCUS       DADMFL010000015        49573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.015, whole genome shotgun sequence.
ACCESSION   DADMFL010000015 DADMFL010000000
VERSION     DADMFL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.015"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1224
                     /locus_tag="JNZ27_000880"
     CDS             <1..1224
                     /locus_tag="JNZ27_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8663312.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JNZ27_000881"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JNZ27_000881"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8663313.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JNZ27_000882"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JNZ27_000882"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY8663314.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JNZ27_000883"
     CDS             2714..3427
                     /locus_tag="JNZ27_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY8663315.1"
                     /translation="MKIELTVNRLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VFFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JNZ27_000884"
     CDS             complement(3399..4076)
                     /locus_tag="JNZ27_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8663316.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JNZ27_000885"
     CDS             complement(4070..4747)
                     /locus_tag="JNZ27_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY8663317.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="JNZ27_000886"
     CDS             complement(4735..5118)
                     /locus_tag="JNZ27_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY8663318.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="JNZ27_000887"
     CDS             complement(5134..5442)
                     /locus_tag="JNZ27_000887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663319.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="JNZ27_000888"
     CDS             complement(5443..6021)
                     /locus_tag="JNZ27_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY8663320.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JNZ27_000889"
     CDS             complement(6044..6475)
                     /locus_tag="JNZ27_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8663321.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JNZ27_000890"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JNZ27_000890"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY8663322.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCSGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JNZ27_000891"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JNZ27_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY8663323.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JNZ27_000892"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JNZ27_000892"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY8663324.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JNZ27_000893"
     CDS             10564..11424
                     /locus_tag="JNZ27_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY8663325.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JNZ27_000894"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JNZ27_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY8663326.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JNZ27_000895"
     CDS             12291..13031
                     /locus_tag="JNZ27_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY8663327.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..13707
                     /locus_tag="JNZ27_000896"
     CDS             13198..13707
                     /locus_tag="JNZ27_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8663328.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPL"
     gene            13831..14093
                     /locus_tag="JNZ27_000897"
                     /pseudo
     CDS             13831..14093
                     /locus_tag="JNZ27_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNZ27_000898"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JNZ27_000898"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY8663329.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASINAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNZ27_000899"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JNZ27_000899"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY8663330.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNZ27_000900"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JNZ27_000900"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY8663331.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNZ27_000901"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JNZ27_000901"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY8663332.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /locus_tag="JNZ27_000902"
     CDS             complement(19724..20617)
                     /locus_tag="JNZ27_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY8663333.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="JNZ27_000903"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="JNZ27_000903"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY8663334.1"
                     /translation="MTQDELKKAVGWTALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /locus_tag="JNZ27_000904"
     CDS             21915..23147
                     /locus_tag="JNZ27_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY8663335.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="JNZ27_000905"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="JNZ27_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY8663336.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="JNZ27_000906"
     CDS             23768..23827
                     /locus_tag="JNZ27_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8663337.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="JNZ27_000907"
     CDS             complement(23924..24526)
                     /locus_tag="JNZ27_000907"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY8663338.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="JNZ27_000908"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNZ27_000908"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNZ27_000909"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="JNZ27_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY8663339.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="JNZ27_000910"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="JNZ27_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY8663340.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="JNZ27_000911"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="JNZ27_000911"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY8663341.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="JNZ27_000912"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="JNZ27_000912"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY8663342.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQVHERGEDIGDYGILCRYQQ
                     RRQSDCEATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="JNZ27_000913"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="JNZ27_000913"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY8663343.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="JNZ27_000914"
     CDS             complement(29672..29755)
                     /locus_tag="JNZ27_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663344.1"
                     /translation="MSKNTKSKNHDIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="JNZ27_000915"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="JNZ27_000915"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY8663345.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="JNZ27_000916"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="JNZ27_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY8663346.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="JNZ27_000917"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="JNZ27_000917"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY8663347.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="JNZ27_000918"
     CDS             34812..35555
                     /locus_tag="JNZ27_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8663348.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /locus_tag="JNZ27_000919"
                     /pseudo
     CDS             complement(35612..37045)
                     /locus_tag="JNZ27_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            37090..37401
                     /locus_tag="JNZ27_000920"
     CDS             37090..37401
                     /locus_tag="JNZ27_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY8663349.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="JNZ27_000921"
     CDS             37565..38224
                     /locus_tag="JNZ27_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY8663350.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNZ27_000922"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="JNZ27_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY8663351.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="JNZ27_000923"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="JNZ27_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY8663352.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="JNZ27_000924"
     CDS             39890..40297
                     /locus_tag="JNZ27_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663353.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNZ27_000925"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="JNZ27_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY8663354.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="JNZ27_000926"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="JNZ27_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY8663355.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="JNZ27_000927"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="JNZ27_000927"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY8663356.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVSGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="JNZ27_000928"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="JNZ27_000928"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY8663357.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="JNZ27_000929"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="JNZ27_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY8663358.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="JNZ27_000930"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="JNZ27_000930"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY8663359.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNZ27_000931"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="JNZ27_000931"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY8663360.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="JNZ27_000932"
     CDS             47702..47773
                     /locus_tag="JNZ27_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663361.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="JNZ27_000933"
     CDS             complement(47770..47895)
                     /locus_tag="JNZ27_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663362.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /locus_tag="JNZ27_000934"
     CDS             complement(47897..49345)
                     /locus_tag="JNZ27_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY8663363.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11967 a        12403 c        13345 g        11858 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcgggaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatagg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtgtt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg tgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgctccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc ctggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gttgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatggacggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac taggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccaggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 tgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga ttgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgtcatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaagcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggcatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgcgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc taggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tagtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttt cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggcgaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gagaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg cat
//
LOCUS       DADMFL010000016        48169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.016, whole genome shotgun sequence.
ACCESSION   DADMFL010000016 DADMFL010000000
VERSION     DADMFL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.016"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            154..768
                     /locus_tag="JNZ27_000935"
     CDS             154..768
                     /locus_tag="JNZ27_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY8663364.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            768..1097
                     /locus_tag="JNZ27_000936"
     CDS             768..1097
                     /locus_tag="JNZ27_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY8663365.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1109..1999
                     /gene="cyoE"
                     /locus_tag="JNZ27_000937"
     CDS             1109..1999
                     /gene="cyoE"
                     /locus_tag="JNZ27_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY8663366.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FSANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            2184..2495
                     /locus_tag="JNZ27_000938"
     CDS             2184..2495
                     /locus_tag="JNZ27_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8663367.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            2479..3021
                     /locus_tag="JNZ27_000939"
     CDS             2479..3021
                     /locus_tag="JNZ27_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8663368.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(3077..4012)
                     /locus_tag="JNZ27_000940"
     CDS             complement(3077..4012)
                     /locus_tag="JNZ27_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8663369.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(4023..5525)
                     /locus_tag="JNZ27_000941"
     CDS             complement(4023..5525)
                     /locus_tag="JNZ27_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8663370.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGEGVSKEYTLSVPPFSLNTLGVDMYNNSSY
                     IRKSYIDIPMYNRNLCDFHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            5919..7289
                     /locus_tag="JNZ27_000942"
     CDS             5919..7289
                     /locus_tag="JNZ27_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663371.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADSGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            7471..9625
                     /locus_tag="JNZ27_000943"
                     /pseudo
     CDS             7471..9625
                     /locus_tag="JNZ27_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9678..11408
                     /locus_tag="JNZ27_000944"
     CDS             9678..11408
                     /locus_tag="JNZ27_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663372.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(11468..11959)
                     /gene="yajQ"
                     /locus_tag="JNZ27_000945"
     CDS             complement(11468..11959)
                     /gene="yajQ"
                     /locus_tag="JNZ27_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY8663373.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            12127..13038
                     /gene="panE"
                     /locus_tag="JNZ27_000946"
     CDS             12127..13038
                     /gene="panE"
                     /locus_tag="JNZ27_000946"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY8663374.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            13001..13591
                     /gene="yajL"
                     /locus_tag="JNZ27_000947"
     CDS             13001..13591
                     /gene="yajL"
                     /locus_tag="JNZ27_000947"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY8663375.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(13645..15093)
                     /gene="thiI"
                     /locus_tag="JNZ27_000948"
     CDS             complement(13645..15093)
                     /gene="thiI"
                     /locus_tag="JNZ27_000948"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY8663376.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            15299..15541
                     /gene="xseB"
                     /locus_tag="JNZ27_000949"
     CDS             15299..15541
                     /gene="xseB"
                     /locus_tag="JNZ27_000949"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY8663377.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            15541..16440
                     /gene="ispA"
                     /locus_tag="JNZ27_000950"
     CDS             15541..16440
                     /gene="ispA"
                     /locus_tag="JNZ27_000950"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY8663378.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            16465..18327
                     /gene="dxs"
                     /locus_tag="JNZ27_000951"
     CDS             16465..18327
                     /gene="dxs"
                     /locus_tag="JNZ27_000951"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY8663379.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            18382..19356
                     /gene="yajO"
                     /locus_tag="JNZ27_000952"
     CDS             18382..19356
                     /gene="yajO"
                     /locus_tag="JNZ27_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY8663380.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(19410..19928)
                     /gene="pgpA"
                     /locus_tag="JNZ27_000953"
     CDS             complement(19410..19928)
                     /gene="pgpA"
                     /locus_tag="JNZ27_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY8663381.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(19906..20883)
                     /gene="thiL"
                     /locus_tag="JNZ27_000954"
     CDS             complement(19906..20883)
                     /gene="thiL"
                     /locus_tag="JNZ27_000954"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY8663382.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(20961..21380)
                     /gene="nusB"
                     /locus_tag="JNZ27_000955"
     CDS             complement(20961..21380)
                     /gene="nusB"
                     /locus_tag="JNZ27_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY8663383.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(21400..21870)
                     /gene="ribE"
                     /locus_tag="JNZ27_000956"
     CDS             complement(21400..21870)
                     /gene="ribE"
                     /locus_tag="JNZ27_000956"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY8663384.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(21962..23062)
                     /gene="ribD"
                     /locus_tag="JNZ27_000957"
                     /pseudo
     CDS             complement(21962..23062)
                     /gene="ribD"
                     /locus_tag="JNZ27_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(23066..23515)
                     /gene="nrdR"
                     /locus_tag="JNZ27_000958"
     CDS             complement(23066..23515)
                     /gene="nrdR"
                     /locus_tag="JNZ27_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY8663385.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            23666..24205
                     /locus_tag="JNZ27_000959"
     CDS             23666..24205
                     /locus_tag="JNZ27_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY8663386.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            24504..25388
                     /locus_tag="JNZ27_000960"
     CDS             24504..25388
                     /locus_tag="JNZ27_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY8663387.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(25565..25912)
                     /locus_tag="JNZ27_000961"
     CDS             complement(25565..25912)
                     /locus_tag="JNZ27_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY8663388.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(26041..27012)
                     /gene="secF"
                     /locus_tag="JNZ27_000962"
     CDS             complement(26041..27012)
                     /gene="secF"
                     /locus_tag="JNZ27_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY8663389.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(27023..28870)
                     /gene="secD"
                     /locus_tag="JNZ27_000963"
     CDS             complement(27023..28870)
                     /gene="secD"
                     /locus_tag="JNZ27_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY8663390.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(28898..29230)
                     /gene="yajC"
                     /locus_tag="JNZ27_000964"
     CDS             complement(28898..29230)
                     /gene="yajC"
                     /locus_tag="JNZ27_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY8663391.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(29253..30380)
                     /gene="tgt"
                     /locus_tag="JNZ27_000965"
     CDS             complement(29253..30380)
                     /gene="tgt"
                     /locus_tag="JNZ27_000965"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY8663392.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(30435..31505)
                     /gene="queA"
                     /locus_tag="JNZ27_000966"
     CDS             complement(30435..31505)
                     /gene="queA"
                     /locus_tag="JNZ27_000966"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY8663393.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            31598..32171
                     /gene="acpH"
                     /locus_tag="JNZ27_000967"
                     /pseudo
     CDS             31598..32171
                     /gene="acpH"
                     /locus_tag="JNZ27_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(32176..33993)
                     /gene="malZ"
                     /locus_tag="JNZ27_000968"
     CDS             complement(32176..33993)
                     /gene="malZ"
                     /locus_tag="JNZ27_000968"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY8663394.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(34149..35522)
                     /gene="proY"
                     /locus_tag="JNZ27_000969"
     CDS             complement(34149..35522)
                     /gene="proY"
                     /locus_tag="JNZ27_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY8663395.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(35598..36917)
                     /gene="brnQ"
                     /locus_tag="JNZ27_000970"
     CDS             complement(35598..36917)
                     /gene="brnQ"
                     /locus_tag="JNZ27_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY8663396.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(37324..38619)
                     /gene="phoR"
                     /locus_tag="JNZ27_000971"
     CDS             complement(37324..38619)
                     /gene="phoR"
                     /locus_tag="JNZ27_000971"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY8663397.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(38677..39366)
                     /gene="phoB"
                     /locus_tag="JNZ27_000972"
     CDS             complement(38677..39366)
                     /gene="phoB"
                     /locus_tag="JNZ27_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY8663398.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            39556..40758
                     /gene="sbcD"
                     /locus_tag="JNZ27_000973"
     CDS             39556..40758
                     /gene="sbcD"
                     /locus_tag="JNZ27_000973"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY8663399.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            40755..43898
                     /gene="sbcC"
                     /locus_tag="JNZ27_000974"
     CDS             40755..43898
                     /gene="sbcC"
                     /locus_tag="JNZ27_000974"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY8663400.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            44024..45205
                     /gene="araJ"
                     /locus_tag="JNZ27_000975"
     CDS             44024..45205
                     /gene="araJ"
                     /locus_tag="JNZ27_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY8663401.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(45348..46256)
                     /gene="mak"
                     /locus_tag="JNZ27_000976"
     CDS             complement(45348..46256)
                     /gene="mak"
                     /locus_tag="JNZ27_000976"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY8663402.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRQVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            46381..47292
                     /gene="rdgC"
                     /locus_tag="JNZ27_000977"
     CDS             46381..47292
                     /gene="rdgC"
                     /locus_tag="JNZ27_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY8663403.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKQEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(47370..47825)
                     /locus_tag="JNZ27_000978"
     CDS             complement(47370..47825)
                     /locus_tag="JNZ27_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663404.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(47822..>48169)
                     /locus_tag="JNZ27_000979"
     CDS             complement(47822..>48169)
                     /locus_tag="JNZ27_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY8663405.1"
                     /translation="LQVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPAS
                     LLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRK
                     IRSEARHRLEEKQ"
BASE COUNT        12464 a        12365 c        11705 g        11635 t
ORIGIN      
        1 gggcgcttac ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
       61 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
      121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
      181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
      241 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
      301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
      361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
      421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
      481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
      541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
      601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
      661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
      721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
      781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
      841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
      901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
      961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     1021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     1081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     1141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     1201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     1261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     1321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     1381 cttgctgggt attgctggct ttatgctgct gtggtttagc gcgaatccgc tggcctgctg
     1441 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     1501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     1561 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     1621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     1681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     1741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     1801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     1861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     1921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     1981 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttta
     2041 ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga gcagatgcac
     2101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     2161 ttcagttaat caaagaagtc ataatgcctg gaaaaacagc cgctctatac gatgtcgata
     2221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     2281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     2341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     2401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     2461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     2521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     2581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     2641 gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg tacttttacg
     2701 gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     2761 attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa gcggggcttt
     2821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcacca ggcattacca
     2881 attaaagggg tttatcttga tgccgaccct gccaccatta acttttatgc tcgtctcggc
     2941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     3001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgccttagc
     3061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     3121 ccttgccgtt aatagttgcc gttgcggcaa ctattgtggt ccttacagtt gcacaagatt
     3181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     3241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     3301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga ttggccactt
     3361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct ttatctaaaa
     3421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcc tcaaccgttt
     3481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     3541 ggacaccttt atcaaggtat tccttcgctt tcttacaatc tacctcaaca tactgaccat
     3601 tggcgtacat catgcccaac ctggaccatg ctaaattcat accttgctgc gcggcctgat
     3661 taaatagtgc atacgctttg gcataatcca cccgcacctg ctcaccgtcg tagtagatac
     3721 tccccagcgc taacggagct tctctgtcac cttgtttagc ggctaactcg aaccatttaa
     3781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     3841 ggctctgcgc gtcgatatct cccgattcag ccattttatg cagtgtattt ataacatcgg
     3901 gagcagattt accaccgtat atgctgtcat acaattcatg cgctttctta cttgccacca
     3961 cgcctgatga aactatcagc attaaagaaa ataatattct attaggcaac ataaaatatc
     4021 tcttatttat cttgtacctg aatatgctct tcttttaata aatcacgttg ttttccattc
     4081 agtatcgctg tcgcagattt aatagaataa gtcaaatcat gaaaatcgca taaattacga
     4141 ttatacattg ggatgtctat atagcttttt cttatataac tggaattatt atacatatcc
     4201 acaccgagcg tattcaaact aaaaggaggg acagaaagag tatattcttt actcactccc
     4261 tccccccccc cttttagctc aagcgtcaac tgcaaattag ttatactact aattttatct
     4321 gcattaattt caatactgac atcgcatatt ttgggttttt catttgcgtt aaagccatag
     4381 gtcccgtaat gttttaacgt gatgtaaggt aaagtatcac cctgtgtttc tcgggttttc
     4441 tgatcttgat tacaacgggt taaatattca tccttattga atttcattga atatttcccc
     4501 gcaccttttt cgtaataatc ccatacctta tcacaatcaa gcgtttcacc taatccgttg
     4561 taatacatcc aacttaacca caatgcggct ttttttgccc cttgctgttc agccttgtca
     4621 aaaagcatgc tcgctttaga ataatcaacc tcaccggtca aaccagaata gtatatattc
     4681 cccagggcca ctaatgcttt ttcgtcattc ttatttgcag ctaattcgta ccattttttg
     4741 gcttcgacag catcaatctt taaaccagca ctgccatttt cgtaggtatc gcccatggta
     4801 tactgcgcca tcggataacc tttttctgcc gcagtacgaa accattgcag tgctttcttt
     4861 atggtgtcta aagaacgtct atcatcagaa ggtgaaaaat catcgagata aagatttccg
     4921 acaatataat ccatttcggg gtcgccttcc tgcgcccata gagtcgccag acgcagaatt
     4981 tcaggtttac tgaaaccaat tcgcccccaa aaaagcaaat cattctcggg tgcctgaccg
     5041 acataacgac tgacaaattc aattaaactc gcccgtttcg ttttgtctgt ctcagccccg
     5101 gatttcaatt cataggcctg attgattatt ttattcatca aatcaggcgg aatattaaga
     5161 tcgtcagttt tcgacttact ggcatacttc tgtgctcgtg tcacatctag ggtgacgcct
     5221 ttaccatatt gaaagaaata acgtgcatta taacaaccgt attcgccccc ctggttacag
     5281 gctttctcat accactgaat cgcctttggg atgtcaacag atactgtcac tccgtgttca
     5341 taaataaatc ccagtagcac tagcgcctgc gtgttattgg tatcagctaa ttttttcagt
     5401 tcttttaacg ccgtctgatc tttattgaca atacgttcat acagttgatc aatatctgtt
     5461 gaagcatgac aaaaagaagc cagagataaa cccaatgata ataaaataac tttaccagac
     5521 cacatacatt cctccttgat atattcaacc accaaacatc cacctcatga ataaatacgc
     5581 tgccataacg gcccaggtaa taagtaaaca tttaagctga aacagtataa ccagtgcctc
     5641 caacgcttct atttattgcg ataaaataac accatccttt gataaaaagt cacacgccca
     5701 tcaataaatc caaaaataat gtcattagtt gcaggataaa atcatttgag cttttcattt
     5761 gttatattta acgcatgatg tattaatggc aagaatatga gagaaactca tacctgacgt
     5821 aaaaatcact ctcttatgta atatttactt attaacactc tgtttaccta acatgcgctc
     5881 cgcactacac tatgtgggca attttttaag aggtggtaat gaacgattat aaaatgacgc
     5941 caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt ttcattgcgc atgctgggca
     6001 tgtttattgt actgccggtt ctgactacgt acggtatggc actgcaaggc gccagcgaag
     6061 cattaatcgg tattgccatt ggtatttatg gtctgaccca ggccgttttt cagattccgt
     6121 ttggcctgct ttccgaccgc attggtcgaa aaccattaat tgtcggtggg ctggcagtgt
     6181 ttgccgccgg tagcgttatc gctgcgcttt ctgactccat ctggggaatt attctgggcc
     6241 gggcgctaca aggctccggt gcgattgccg ctgccgttat ggcgctgctt tccgatctca
     6301 cgcgcgaaca aaaccgtacc aaagcaatgg cgtttatcgg cgtgagcttt ggcattacct
     6361 ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt
     6421 tctggatgat cgctattctg gcaacgaccg gcattgcgtt gaccatttgg gttgtgccca
     6481 acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaaggcagt ttcagtaaag
     6541 tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc
     6601 tgatgtcgac gtttgttgcc ctgcccggac aactggctga ttcggggttc ccggcggctg
     6661 aacactggaa ggtctatctg gcgacaatgc taatcgcctt tggctcggtc gtgcctttca
     6721 ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttctgc gtcgggttga
     6781 tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca gttctggcaa ctggtggtcg
     6841 gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc cctgctgccc tcacttatca
     6901 gtaaagagtc gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt
     6961 ttcttggcgt ggcgattggc ggttcgctgg gcggctggat tgacggcatg tttgacggtc
     7021 agcgggtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca
     7081 tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat cccagaaaac gtaatggcag
     7141 atgaaaaact ggaaaaatgc ttactgacaa cgcctggtgt caaagatgta ctgctggcga
     7201 agttagaaca ttgcgtctat gtgaaagtag acagtaaaat caccaatcgt tttgaaatag
     7261 agcgagtaat caacgaagta tcttattaaa taataagcac ggtgctattc atgaaaaata
     7321 aaatagcacc gtacttcgta acaccataat cctttaaacc ctccattatt aatacttcgc
     7381 ttaattccat ataactggac gattacatta gaagtaaact atacttttta tgaaatatca
     7441 gggataaatt attcacatgg aaaatacatc atgtacaaat cattatttac tattatcatt
     7501 ctactgtata gcgccagttc atatgcaaaa agcgaatccc ctcccgcaag cactataact
     7561 ttcattgcac aaatcgatca tgttgagcta aataaaacag cattatctca tgatatgaaa
     7621 ctcgttcttg ccgatcgttt ttatttcaaa accagtaatc cttgcgaaac aaattcaatt
     7681 tctgctcgac cagaatcaat ggatattact gacaaagcct atgccaacaa attaaaaaaa
     7741 ttagaaactc cttttcttga tgatcgctat ttctttatga ccatcaatca gtgtggcggt
     7801 aatggcactc ccatgctgac agacataaaa gtatgtacaa cagaattatg tggtgctgaa
     7861 tatacaaaga aaatgtctac tgtatggctc gatgaaactt taaaaccaac tgctaaacgt
     7921 ctagcaattt attttattca atctccttta ccctatgacg aggagaaaaa acaatggaaa
     7981 gtcaccggat ggtatatctc atcaactgat aatcagtctt caactaaaca gattgcattt
     8041 gaaggatata cagacacaga taacttctcc gattctaaat ttatttcttc atttaaatcc
     8101 tggtttgaaa gtggaaagat aaaaaacatt gctaactata atccaaaagg agaaagagat
     8161 ggaaaatacg aaagttatta caatagcaat ggtaacgtct atgaatctct aagtttttct
     8221 aatggtctta tcaacggtga gtttattaca tattatgaaa atggggccat tgaaagtaaa
     8281 cgtcatttta ataacgacaa aattaaagat ggtgaatgcc ctcattttta tgaagatggg
     8341 aaaatcaaac agcaacatag ctatctgaat agtaaactcg atggtcctgc atatgaatat
     8401 tatcctgatg gcaaactcat gcaggaaaat ttctatcagc aaagtgtgtt gattggaaac
     8461 gataccagtt actaccaatc cggaaaaata agctctatac acaatagaaa ttcccgtggt
     8521 cagtatgatg gagctaacga acgttatagt gaagagggta agttacagtc taaatccgtc
     8581 tataaagacg gaaaacaaat ttctgttcaa acctggtatg aaaatgggca gaaagaagag
     8641 gagaaacatt ttgatgagca aggccaatta aatggtctgg ttaagcaatg gtataaaaat
     8701 ggtaatcttg caaaatcaca aaactataaa catgacatac tcgatggtaa cagtgaagag
     8761 tggtacgaaa atggcattcc tgaaagtttg tacccttata agaatggaaa aaccgacggc
     8821 attgccaaat catggaataa gtatggtaaa ttaacctatt caatcgaata caaaaatggc
     8881 gtagagaatg gggcatatcg taactggagc aaaaacacag gtaaactaac caaagaaacc
     8941 ctgtacgtca atggtatcag acagggagtg gaaaaagagt ttaacgatcg cacaggtaaa
     9001 ctattaacct ccacgcaata tgttaataat aaaagacatg gtacggagga aacttatgat
     9061 caaaacggaa taaaatatat tacatgctat caaaatgatc agaaattatc gtctttagac
     9121 aatccaacac aaataaaaga taatgcaaca acaggcgatt ccagtgctca atttgcatta
     9181 gggaaatacg aattcatctg tgcaaatata gatgaaggga ttaaatggtt aaccaaatct
     9241 gcagagcaaa aaaaacactg atgcgatata ctttttagct acagcctaca aaggcaatgg
     9301 tatacctgca aacaatgaaa aatacctagc atatctgcaa caagctgcaa cactgggcag
     9361 tagtaatgcc caggcggaaa ttggctattt atatctcatc ggaaaagaac taccacagaa
     9421 tcttcctgat gctggtgtct ggttcaaaaa agcagctgca caaggtaatt ttgccgcaca
     9481 tttctatctg ggaaggatgt atcagaatgg tgacggcgtt gagagaaata tggaaaaagc
     9541 caggtttcat ctatctaatg cggcagaagg tggaatagaa cctgcgctta aagcattaaa
     9601 tgaacttgaa catcaaacaa aataaagcgc tcagggacca cgtaaggttc atcaaacaga
     9661 aaaggatata gtttgcgatg aaagcgaaat ctactttgtt attaatgatt ttatctgttt
     9721 tttattctca tcaggtttta tccagcgacc ccactcagcc ggatgtttat tctgtcgtcg
     9781 agaaaaaact aaactctgct ttacctctgg aagaaaatac caccttcaga tcgcaggctg
     9841 aatggtttaa tttgcaatat gaattattag tatctggcta ccctgaacgt gcttacaatt
     9901 tactgagcaa acttgagaca gaatcgaaaa acgctaaata ttatctggat ttaactgcct
     9961 ccagagtcgc tgaagaagaa gcaccagaga cagccttaac ttttttatca aaaattgggt
    10021 taaataaacc ggctactctt acatcctttg agagctatat caatagctgg ataaaaagca
    10081 aacaacccga aaaggctatg cagcttctca gcctcgatga gaatgccctg aattattatt
    10141 taccgactgt gttagtcgca tatcagatcg ttcctgataa ggcaataagt atttataatt
    10201 ctgtatatgg agataatgtt gttataccct atcagcaagt caccatgtta cttacagttg
    10261 cggagggtta tcaaacaaaa ggcgatataa aaaacgccat tcattatgcc gataaggcgc
    10321 tgaatatgtt tgataaagct attgctgtag atagcagtcg tgaatcccat tatagtgatg
    10381 agtatttgaa actcatgaat atctacgccg ccaatggcaa caaagaaaaa gcggtggttt
    10441 tatctcaacg tctacacaaa gccattaccg aatctcaatc gaattactta agtactctgg
    10501 aaggaatttt attgttttat agaaataatg atatgcagca agactatcaa aacagccttt
    10561 caaattacat agtatttatc gatgaaattt ttgccttttc cccatcaaca agaagtgaat
    10621 taagcctcat taatttatta aattcattaa atgaggtgga attgatgaga aaacgtctgg
    10681 cgtttctgat gacagcacca gaatacgctt gttatgatag tcagtattgt tatgaagaca
    10741 aagtcagcgc attaaaaatc ctgtatcgtc atcatgacaa tgagctaatg gacaaatatt
    10801 tcacgactct tcttaaagag atcagtaatc aaccattaga agactgggat ataataataa
    10861 gtaatactgg tgaaaaattt agtgaagtgg gtctaaccaa atatgctcaa catctcgcat
    10921 ctttcgctga agaattttat ataaaagagc aaaaatcaca ttctgaaagt gaaatgagag
    10981 gactattttc atcacttgcc gaattatata gttttggtaa tgatactgcc agcgcaaacc
    11041 gagtgttcca tcagcatgtc cctgccttca atacagatca tatgattcaa tattttataa
    11101 atgccaaaga atggaacaat gccagggaat tattgataaa agaggaaatg ttgggtatgc
    11161 ataatctgat attgttggag aatatttgta tgcaaaaaaa tgcagaatgc atggcacaca
    11221 ttacctttac gcttaataaa ttaacaacac aaccagcaat aacaaaaata gatagcgtgg
    11281 gtaatgagca actttatcag attggcaaaa tctaccataa gttggaaatt aaacctgagc
    11341 cagagcaaca ggttttaatt cagtcgcttt atgaccgggc atcaggaagc gcctctgcaa
    11401 ctcaataaat gcatggtcaa aagggccacc acggtggccc tgacaatcag tagctatatc
    11461 cagaaagtta atcgcggaag tttttgaact ggaacggctg accgagatcg ccaccgcgca
    11521 ccatcgccat gacagcctgc aaatcatcac gagatttgcc agtcacgcgg atctcatcgc
    11581 cctgaatttg cgcctgcact ttcagtttgc tgtctttgat catcttgacg atttttttct
    11641 gagtggcgct ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt ttaccgctat
    11701 gaacgatatt ttccggtaca tccagtgaac tgccttcaat gccgcgcttc agcagcttgg
    11761 cacgcagaat atccagcaac tgattgacct ggaagtcgga ctcgctcaac actttgatgg
    11821 ttttgctggc gtcgttcagc tcaaatgagg cttcaacgtt acggaagtca aaacgggact
    11881 ccacttcgcg actcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca acttcagaga
    11941 caatatcgaa agatggcatc ttttcctctc ccttcatttt tgatgcgaag cataatacct
    12001 gcaaagttaa ataaccaaca gtcctgcacc aggacgtatc ccttaattgt gcatagtcgc
    12061 aacagcatgc acaattaagg gatagcctta ttgaacaccc ggagtggttg tgggtgagga
    12121 ggaacaatga aaattaccgt attgggatgc ggtgccttag ggcaattatg gcttacagca
    12181 ctttgtaaac agggtcatga agttcagggc tggctgcgcg taccgcaacc ttattgtagt
    12241 gtgaatctgg ttgagacaga tggttcgata tttaacgaat cgctgaccgc caacgatccc
    12301 gattttctcg ccaccagcga tctgctcctg gtgacgctga aagcatggca ggtttccgat
    12361 gccgtcaaaa gcctcgcatc cacactgcct gtaactacgc caatactgtt aattcacaac
    12421 ggcatgggca ccatcgaaga attgcaaaac attcagcagc cattactgat gggcaccacc
    12481 acccatgccg cccgccgcga cggcaatgtc attattcatg tggcaaacgg aatcacgcat
    12541 atcggtccgg cacggcaaca ggatggcgat tacagttatc tggcggatat tttgcaaacc
    12601 gtgttgcctg acgttgcctg gcataacaat attcgcgccg agctgtggcg caagctggcc
    12661 gtcaactgcg tgattaatcc actgactgcc atctggaatt gcccgaacgg tgaattacgt
    12721 catcatccgc aagaaattat gcaaatatgc gaagaagtcg cggcagtgat cgaacgcgaa
    12781 gggcatcata cttcagcgga agatttgcgt gattacgtga tgcaggtgat tgatgccaca
    12841 gcggaaaata tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac cgaaatcgac
    12901 tatatcaatg gttttctctt acgccgcgcc cgcacgcatg ggattgccgt accggaaaac
    12961 acccgcctgt ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat cggcactggt
    13021 ttgcctcgcc cctggtagtg aagagactga agccgtcacc acgatcgacc tcttggttcg
    13081 cggcggtatc aaagtcacca ctgccagcgt cgacagcgat ggcaacctgg caattacctg
    13141 ctcgcgcggc gtgaagctgc tggcggatgc gccgctggtc gaagtggctg atggcgaata
    13201 tgacgtgatc gtgctgcctg gtggcattaa aggcacggag tgttttcgcg atagcactct
    13261 gctggttgaa accgttaaac agttccaccg ttccgggcgt atcgtcgcgg ctatttgcgc
    13321 cgcgccagcc accgtgctgg tgccgcacga tatctttccg attggcaata tgaccggctt
    13381 cccgacgctg aaagacaaaa ttcccgctga acaatggcag gacaagcgcg tcgtctggga
    13441 tgcacgggta aaattgctga ccagccaggg gccgggtaca gctatcgact ttggtctgaa
    13501 aattatcgac ctgctggttg ggcgcgagaa agcccatgaa gtggcatcac aactggtgat
    13561 ggcggcaggg atttataatt attacgagta gtgtcggatg cggcaaacgt cgcatctgac
    13621 cagatgcgac gtaaaaacta caaattacgg gcgatatacc ttcacattgt taaagccttg
    13681 ctcgcgcaga tagagcgcct gcagacggct catcaccccg cgctcacacc acagcagcca
    13741 ggttttgttc tggtcgagat cgccaaattt ggtgctcagt ttatagaacg gcagagaaac
    13801 cacatcaatc ccttcgactt tcagtggctt atcttcctgt tcatcgacag aacggatatc
    13861 gagaatcacg tcgttcgggc cgaagccgtt gacggtttcc acttccacca cttcctgctc
    13921 ggtctgctgg gcgatttcgc ggatatcaac gttattcgct tcctcaacca ctttatcgag
    13981 aatactgaag tcgaacttct cttcttccgc ttcaatcttc gatttaaccg ctttcaccgt
    14041 cgggcttttg gagatcacgc cacaatattc cggcatcgtg cgagcaaagt cttcggtgcc
    14101 aatctggcga gccaggttga tgatgtgctc tttgtcgtaa gaaatcagcg gacgcaggat
    14161 cagcgtgtca gagacgttat caatcaggcg caggttggtc agcgtctgac tggacacctg
    14221 gccgagcgct tcgccggtga ccagcgcctg tacgccgtaa cgttcagcca ctttagatgc
    14281 ggcacgcacc atcatacgtt tgaggataac gcccatctga ccgtcgtcga ttttctcgag
    14341 aatttccccg acaaccggtt cgaaattaat ggcgacaaaa cgcacgcggt gggagctgcc
    14401 aaagcggttc cacagataat gcgccacctg acgcacgcca atttcatgcg ctgcgccgcc
    14461 gaggttaaag aagcagtaat gcacgcggca gccgcgacgc atcaacatat aactggaaac
    14521 accggagtcg aaaccaccgg aaatgagcga cagcacatct tcctgggtgc caatcgggaa
    14581 accaccaata ccttcgtagc gacctttaat cagcaggagg cgatcgtctt ccacttccag
    14641 atgaacagtt acatccgggt tagtcagctt cacgcgcgcg gattcaatat gctgatttaa
    14701 accgccgccg acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc cacggcgctt
    14761 cacgcgtacg cagaaggttt tgccttccag ctgatcgcga tactgaacca acgctttctc
    14821 aaaaatatcg tgcatgtcgg taaacggcac gtcttcgact tcgagaatat ggtggatacc
    14881 cggaatacgg gtcagggcgt cgcgaatagt cagacgctgg ttttcatctt tcgcgcgaac
    14941 ttcgatgtta tcccagtggc ggacgacagc gagcgtctca tcatagtgct ttaaaacgtt
    15001 acgaatgttc ccggtaagga tttttataaa gcgcaagcgc acagattggc ttttgatggt
    15061 gatttccggg aacaatttaa tgataaactt catggcggca atggttcgtt ggcaagcctt
    15121 aagcgacttg tatagggaaa aatacagcag cccacacctg cggctgcatc caggcgcgga
    15181 agtataccac taacatcgct ttgctgcgca catcacctta ccattgcgcg ttatttgcta
    15241 tttgccctga gtccgttacc atgacggggc gaaaaatatt gagagtcaga cattcattat
    15301 gccgaagaaa aatgaggcgc ccgccagctt tgaaaaggcg ctgagcgagc tggaacagat
    15361 tgtaacccgt ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg agttcgaacg
    15421 cggcgtgcag ctggcacgtc aggggcaggc caaattacaa caagccgaac agcgcgtaca
    15481 aattctgctg tctgacaatg aagacgcctc tctaacccct tttacaccgg acaatgagta
    15541 atggactttc cgcagcaatt cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt
    15601 tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca
    15661 ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca tatgtttggc
    15721 gttagcacaa acacgctgga cgcacccgct gctgccgtag agtgtatcca cgcttactca
    15781 ttaattcatg atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg tttgccgacc
    15841 tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt acaaacgctg
    15901 gcgttctcga ttctaagcga tgccaatatg ccggaagtgt cggatcgcga cagaatttcg
    15961 atgatttctg aactggcgag cgccagcggt attgccggaa tgtgcggtgg tcaggcatta
    16021 gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat
    16081 aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa
    16141 gggcgtcgtg ctctgccagt actcgacaag tacgcagaga gcatcggcct tgccttccag
    16201 gttcaggatg acatcctgga tgtggtagga gatactgcaa cgttgggaaa acgccagggt
    16261 gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg
    16321 aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag
    16381 tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa
    16441 acaataagta ttaataggcc cctgatgagt tttgatattg ccaaataccc gaccctggca
    16501 ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc gaaactctgc
    16561 gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc
    16621 gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac cccatttgac
    16681 caattgattt gggatgtggg acatcaggct tatccgcata aaattttgac cggacgccgc
    16741 gacaaaatcg gcaccatccg tcagaaaggc ggtctgcacc cgttcccgtg gcgcggcgaa
    16801 agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggt
    16861 attgcggttg ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt gattggcgac
    16921 ggcgcgatta ccgctggcat ggcgtttgaa gcgatgaatc acgcgggcga tattcgtcct
    16981 gatatgctgg tggtcctcaa cgacaatgaa atgtcgattt ccgaaaatgt tggcgcgctt
    17041 aacaaccatc tggctcagct gctttccggt aagctttact cttcactgcg cgaaggcggg
    17101 aaaaaagttt tctctggcgt gccgcccatt aaagagctgc tcaaacgtac cgaagaacat
    17161 attaaaggca tggtggtgcc tggcacgttg tttgaagagc tgggatttaa ctacatcggc
    17221 ccggtggacg gtcacgatgt gctggggctt atcaccacgc taaagaacat gcgcgacctg
    17281 aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa
    17341 aaagacccga tcactttcca cgccgtgcct aaatttgatc cctccagcgg ttgtttgccg
    17401 aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt gtgcgaaacg
    17461 gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg
    17521 gtcgagtttt cacgtaaatt cccggatcgt tacttcgacg tggctattgc cgagcaacac
    17581 gcagtgacct ttgccgccgg tctggcgatt ggtggataca aacccattgt cgcgatctac
    17641 tccaccttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcaat tcaaaagcta
    17701 ccggtcctgt tcgccattga ccgcgcgggc attgttggtg ctgacggtca aactcaccag
    17761 ggcgcttttg atctctctta cctgcgctgc ataccagaaa tggtcattat gaccccgagc
    17821 gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga tggcccgtcc
    17881 gcggtgcgct acccgcgcgg caacgcggtt ggcgtggaac tgacgccgct ggaaaaacta
    17941 ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggtgatcct taactttggt
    18001 acgctgatgc cagaagcggc caaagtcgcc gaatcgctga acgctacgct ggtcgatatg
    18061 cgttttgtga aaccacttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg
    18121 ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gtagcggagt gaacgaagtg
    18181 ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt
    18241 ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc cggtatggaa
    18301 gccaaaatca aggcctggct ggcataatcc ctactccact cctgctatgc ttaagaaatt
    18361 attcatggca ggagtggcag catgcaatac aaccccttag gaaaaaccga ccttcgcgtt
    18421 tcccgacttt gcctcggctg tatgaccttt ggcgagccag atcgcggtaa tcacgcatgg
    18481 acactgccgg aagagagcag ccgtcccata attaaacgcg cgctggaagg tggcataaat
    18541 ttctttgata ccgccaatag ctattccgat ggtagcagcg aagagatcgt tggtcgcgca
    18601 ctgcgggatt tcgcccgtcg tgaagacgta gtcgttgcca ccaaagtgtt ccatcgcgtt
    18661 ggtgatttac cggaaggatt atcccgtgca caaattttgc gctctatcaa cgacagcctg
    18721 cgacgtctcg gcatggatta tgtcgatatc ctgcaaattc atcgcgggga ttacaacacg
    18781 ccgatcgaag agacgctgga agccctgaac gacgtggtaa aagccgggaa agcgcgttat
    18841 atcggcgcgt catccatgca cgcttcgcag tttgctcagg cgctggaact acaaaaacag
    18901 cactgctggg cgcagtttgt cagtatgcag gatcactata atctgattta tcgcgaagaa
    18961 gagcgcgaga tgctgcctct gtgttatcag gagggcgtgg cggtgattcc gtggagcccg
    19021 atggcgcggg ggcgactgac gcgtccatgg ggagaaacta ccgcacgact ggtgtctgat
    19081 gaggtgggga aaaatctcta taaagaaagc gatgaaaatg acgcgcagat cgcagagcgg
    19141 ttaacgggcg tcagtgaaga actcggtgca acacgagcac aagttgcgct ggcctggttg
    19201 ttaagtaaac cgggcattgc cgcaccgatt atcggaactt cgcgggaaga acagcttgat
    19261 gagctgctga acgcggtgga tatcactttg aagccagaac agattgccga gctggaaacg
    19321 ccgtataaac cgcatgcggt cgtaggattt aaataagagt ccaggcctga tgagacgtga
    19381 caagcgtcac atcaggcatc ggtgcacaac tacgacagaa tacccagcgg ccagtgatga
    19441 ccgataaagt acaggatgcc tgcggaaatc accccggcga caatatcgtc gatcatgatc
    19501 cccatgccgc catgtacatt gcgatcaaac cagcggatcg gccacggctt ccacatatcc
    19561 agaatacgga aaatcacaaa cccggcggca acccactgcc agtcattggt cggcagcgcc
    19621 atgagcgtga tccacatacc aataaattcg tcccagacaa tgctgccgtg atcgtgcacg
    19681 cccatatctt tcgccgtttg atgacaaaga tagacgccga tacagatccc caacatcacc
    19741 accagcgagt agagctgcca gggtaaaaag gtcatcagat accagaaagg gatcgccgcc
    19801 agcgatccca tcgtgccagg aacgattggg cttaatccac ttccgaatcc gacagcaagc
    19861 agatgccacg gattactcat cttcaggcga cttttcgcga catctttatg gcgtgacaaa
    19921 atggtcatat cctttccagt caaacgtgac aggttcgccg tcacgaataa aacaaagccc
    19981 ttcgatatcg gcggtcattt gcccgataca ggtaaacggt acgccaaggt gtccgagagc
    20041 aacatccagc gcgccacggt tcagttccgg caccgtgaaa cacaactcgt aatcttcacc
    20101 gccagagagc gcccagcgca gcgcctgttc cggttcaaca tggcgagaaa gcgcatcaga
    20161 aaacggcagc aatgccaggt caatacgtgc gccgcagtcg ctggctttca cgatatgccc
    20221 gagatcggaa atcagaccgt cagagagatc gatagctgaa tttgccagat cgcgcagtgc
    20281 ctgcccctgt aaaatacgcg gcgatggacg gagatgacgt ttgatcaagt agtcagcatc
    20341 tttagcatcg gcaacctgca aacggttttg caaaatcgcc agcccggcgg cgctatcgcc
    20401 cggtgtaccg gtcacataga tccagtcacc cggtttcgcc ccagagcgcg ttaaggctcg
    20461 tcccatcgga acaaagccgt ggatacccaa cgtcattgat aatggcccac gcgtggtatc
    20521 gccgccaatg agttgcatat cgtaataatt gagaagatca aacaaactgt cgctgaagga
    20581 ctcaagccac gcttcgtcta cgtccggtaa ggttaatgcc agcgtcagcc aggccggatc
    20641 ggcccccatc gctgccagat cgcttaggtt caccgccagt gctttataag ccagatcagc
    20701 aggatcgata tcagggagga aatggttgcc cgccaccagc gtatcagtgc tgatcgccag
    20761 ggtctgtttc tcggggatat tgagaagagc gcagtcgtcg ccgatgccca gttcgacatc
    20821 aagacgagaa cttcttacac ggtcaaaata acgggcaatc agggagaact cgccacatgc
    20881 catgcgttat gcctcagcag gtaaaaaaga aaaggccggc gatcatggaa cagtcttccg
    20941 tgaatctacc ggcctggata ttactttttg ttagggcgaa tcacaggtgc tgctttatcg
    21001 agtacgccgt tgacgaactt atggctgtct tctgcgccga acgatttcgc cagttcgatc
    21061 gcttcgttaa tggccacttt gtatggcaca tcactacgtt tagacagttc gtacagcgca
    21121 atgcgcagta ctgctttttc tacctggccc agttcttcca gcaggcggga caggtagggc
    21181 ttcatcagtc cgtcgaggta tgcggtgtta gtcgccaccc cggccagcag ctcacggaag
    21241 tacaggacgt caacgtcttt tacatcctgt tcagccagga actggtattc aacatcagcg
    21301 atgtcgttct gggacaactg ccaggagtag agcgcctgga cggcacactc acgagcgcgg
    21361 cgacgagcag caggtttcac ggatttcccc ttactaattt caggccttga tggctttcaa
    21421 tacattaatc atttcaagcg cggtcagtgc agcttctgca cctttgttgc cagctttggt
    21481 gccagcacgt tcgatcgctt gttcaatgct ttcagtggtc agaaccccaa aagcaaccgg
    21541 aatttcgctg tcctgggcaa catgcgccag gccgttgctt gcaccaccag cgacatattc
    21601 aaagtgggca gtgccaccac gaataaccgt acccagcgca atcaccgcgt cgtatttacc
    21661 ggttttagcc agtgcacccg ccgccagcgg cagctcatag gcaccaggca cccaaacaac
    21721 ggtaatgttt tcatctttta cctgaccgat acgtttcagt gcgtcaattg caccttccag
    21781 caggctgtca ttgataaagt tgttgaaacg cgcgatggtg atggcgacgc gagcgtccgg
    21841 ggtagcaacg ttagcttcaa taatgttcat tttcttcctt cgggttcgag tatggccccg
    21901 caggggggcg gattttagca taatatttcg tgcgctgctt ccctttcgag ccgggagagc
    21961 atgcccccac taaatgcagg caaacatccg ggcctacatg acgtatctct ttgaatttaa
    22021 attggggggc gtcggctaat ttctcaagcc ctggcagcga gcataatcca cgggcgtcgc
    22081 tgcctaatag tttaggtgcg atatagacaa tcagttcatc gactaaaccc gcctgtagca
    22141 atacgccagc gagcgttggc cccgcttcca cccagatgct gttaatctgc tgtttaccca
    22201 gttgcatcat cagtacaacc agatccagat gacctttatg ctctggaatc agcaaggtac
    22261 gcaccgtttc cggccactca cgagaatctt cctgcgtacg cgcgaaccag gtttcgccgg
    22321 gctgctgcac aatgcgatgt tccggcgtca cgcgattttg gctatcaatc acgatacgta
    22381 ctggctggcg gagattttgt tgcgggtaga gcgcctgagt ttgttcatcc agttcagacc
    22441 aacgcactgt taaggctgga tcatccgcca gcaccgtggc gctgctggtt aaaatggcat
    22501 gactttgtgc gcgctgtcgt tgtacgtcgc gccgcgcctg aggcgaagtg atccactggc
    22561 tttcgccgct cgccatcgcc gtgcggccat caagcgatgc gccaagtttt aactgaatat
    22621 aaggaaagcc ggtgcgcatc cgcttgagaa agcctttatt caattgctcg gcttcactca
    22681 tcatcagccc gtggctgacg tcaatgccag cctgttgcag acggtaaagt ccacgcccag
    22741 cgacctgcgg gttaggatct tgcatcgctg cgaccacgcg cgctacgcca gcggcgatga
    22801 gtgcgtcaca gcacggtggc gtacggccat gatggctaca gggttcgagt gtcacatacg
    22861 ccgttgcgcc tttggctttt tcacccgcca tacgcaacgc gtgtacttcg gcatgtggtt
    22921 cacccgctcg ttggtggtaa ccttcaccga caatttcgcc atctttgaca atgacgcacc
    22981 cgacattcgg gttggggtgg gtggtaaaac gtcctcgttg cgccagcttt agcgcccgcg
    23041 ccatgtaata ctcgtcctgc acggcttagt cctccaggcg cgcgatctct tcgccaaatt
    23101 ctttgatatc ttcgaaactg cggtagacag aggcaaaacg gatataggcg actttatcga
    23161 gctttttcaa ttgctccatc accagattgc caatcatctt gctcggtact tcgcgctcac
    23221 cggtggcgcg cagctgcgat ttaatatgat tgattgccat ttcaacgtaa tcggaactca
    23281 ccggacgttt ttccagcgcc cgcagcattc cgctacgcaa tttctcttca ttaaacggtt
    23341 cacgcacgtc gttgcttttt acaacacgcg gcataaccag ctccgccact tcaaaggtgg
    23401 tgaaacgttc attacacacc agacactgcc gacggcggcg tacggatgaa ccctcgccca
    23461 cgagacgaga gtcaattacc ttagtgtcca cggcgaaaca gaatgggcaa tgcatacggc
    23521 gtcctgacca ggtggttaac ggaaatctat tttaccctga actgacgata caacaaaggc
    23581 gcaacgcttt tgagataaag gatcgatgct ttcgctgctt ttaccctcta ccattaggct
    23641 aattgcgacg agaaagggac gacctatgaa tactaacgtt tttcgactgc tcctgctggg
    23701 aagcctgttc agccttagcg cctgtgtgca gcaaagtgaa gtgcgacaga tgaaacacag
    23761 cgtcagcacg ctgaaccagg agatgacgca actcaatcaa gaaacagtca aaatcactca
    23821 gcaaaacagg ctgaatgcaa aatccagcag tggggtttac cttctgcctg gagcgaaaac
    23881 accggcaaga ctggaaagcc agatcggtac tttacgtatg tcgctggtga atattacgcc
    23941 tgatacagat ggcaccaccc ttacactacg tattcagggt gagtcgaatg atcccttgcc
    24001 cgcattcagc ggaactgttg aatatgggca aattcaggga acaatagaca actttcagga
    24061 aatcaatgtg cagaatcaat tgattaatgc ccctgccagc gtcctcgccc ccagcgatgt
    24121 tgatattccg ttacaattaa aggggatttc tgtggatcag ttaggctttg tacgtatcca
    24181 cgacattcaa cctgttatgc agtaaacgta tttcgggacg attttgtgcc tcccgcaaca
    24241 tctttccccg tcattttgtt actctgctta catcacttgg attgatagta aaagtttgca
    24301 acaagggcga aagtcagtac aatccccgcc cgaatgtgtg taaacgtgaa cgcaatcgat
    24361 tacgtaaatg atagaactgt gaaacgaaac atatttttgt gagcaatgat ttttataata
    24421 ggctcctctg tatacgaaat atttagaaac gcaatttgcg cctttttcac tcccgcaagg
    24481 gattttcaaa cagtggcata catatgaaaa aaacattact ggcagccggt gcggtactgg
    24541 cgctctcttc gtcttttact gtcaacgcag ctgaaaacga caaaccgcag tatctttccg
    24601 actggtggca ccagagcgtt aacgttgtcg gaagctatca cacccgtttc ggaccgcaga
    24661 tccgcaacga tacctacctt gagtacgaag cattcgctaa aaaagactgg ttcgacttct
    24721 atggttatgc ggatgcgccg gtattcttcg gcggtaactc cgatgctaaa ggtatctgga
    24781 accacggttc tccgctgttt atggaaatcg aaccacgttt ctccatcgac aagctgacca
    24841 atactgacct tagcttcggt ccgttcaaag agtggtactt cgcgaacaac tacatttacg
    24901 acatgggtcg taataaagat ggtcgccaga gcacctggta catgggtctg ggtaccgata
    24961 tcgacactgg cctgccgatg agcctgtcca tgaacgtcta tgcgaaatac cagtggcaga
    25021 actatggcgc agcgaacgaa aacgagtggg acggttaccg tttcaaaatt aaatactttg
    25081 tgccgattac cgatctgtgg ggcggtcagc tgagctacat cggcttcacc aacttcgact
    25141 ggggttccga tttaggggat gacagcggta acgcaatcaa cggtattaag acccgtacta
    25201 ataactctat cgcttccagc catattctgg ctctgaacta cgatcactgg cactactctg
    25261 tcgtagctcg ttactggcac gacggtggtc agtggaacga cgatgcagaa ctgaacttcg
    25321 gcaacggcaa cttcaacgtt cgctctaccg gctggggtgg ttacctggta gtaggttaca
    25381 acttctgatt atgaaaatgc cgggatttat tcccggcatt tctgattgtt aacaacgtgt
    25441 gctttgttca tgccggatgc ggcgtaaacg ctttgtccgg cccacaaaag cacgtaaatt
    25501 caatatattg ctgaaataac gtaggcctga taagcgtagc gcatcaggca attttacgtt
    25561 ttaatcactt cttcttgttc atcatcgctt tcaggtcagc gaatgggttg tacttcgctt
    25621 caccgacatc tttctgcgca tcttcccctg cgataacggt cgtaccatac tgatccgctt
    25681 cggtatattt cgaatgctca tgatcgtggc aatagagaca caacaattcc cagttactgc
    25741 catcttccgg gttattggta tggtcgtgat caatgtggtg aacggtaagt tcacgcaggt
    25801 tagaataaac aaactcgcgg gaacagcgac cgcagaccca cggatagatt tttaatgctt
    25861 tttcgcgata gccgctttct aaccgtgcat agttttttgg gatgatagcc atgaaaagtt
    25921 acctggatga aatgtagggt tacgcgcagt ttcccaagat gcggggattt tcgcaatctc
    25981 catacatcaa aaaaaatccc gatcttctga ccgggatttt caacatcaac gggaacttga
    26041 ttaaggcaga attgacggct gatccgcccc ttctttttcc actttctgct gcaacatgtg
    26101 ttcgcgcttc atacccagtt tcagagccaa cgcagatgct acgtagatgg aagatgcagt
    26161 gccgatggaa acaccgataa gcatggtcag cgagaagcct tccagtaccg gaccaccgaa
    26221 gagatacagc atcaggataa ccatcaaggt agtaccggat gtgatcaagg tacggtgcag
    26281 cgtctgggtc aaggacacgt taaagatttc gtaaggcgta ccgcgacgga tcttgcggaa
    26341 gttttcacga attcggtccg ataccacgat actgtcgtta agcgagtaac cgataaccga
    26401 catcaacgat gccacaatgg tcaggtcaat ctcgatatgg aataacgaca aaatacccag
    26461 cgtaatgata acgtcgtgcg ccagcgcaat aaccacccct gccgccagtc gccactcaaa
    26521 gcggaaacct acgtacacga ggatagacag cagcgctgcc atcaacgcca tcgcaccggt
    26581 ttgcgcaagg tctgccccca cgctcggacc gacgaactca atacgcttca ctgctgcatt
    26641 ctgattggtg gattcgttaa tcaccttcag aacttggctg cccagcacct gaccgccggt
    26701 ttcgccttca gcaggcggca tacggaccat gatgtcgtgg ctgctaccaa agttttgcag
    26761 cattggctct tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa tttcagccgg
    26821 tttttcgagc gtaatttcga taaccgtacc accggtgaaa tccagccccc agttaaagcc
    26881 gcgcacgccc ataataacga tagcagcgat taacagcaga ccagagatgc cgaaagccca
    26941 gtagtcccag cgcataaagt catagacttt acggccgtgg tttagttgtt caacagtata
    27001 ttcctgtgcc acatcgcact cctcagattg acagcttctt gacgcgcttg ccgccatata
    27061 gcaggtttac gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc gccacaccga
    27121 taccggtagt aatcgcgaac cctttaattg ccccggtacc cactgcgtac aggatgatga
    27181 ctttaatcag cgtggtgatg ttcgcatcga agatagaact gaatgcgcca cgataacctt
    27241 catcaattgc ctgttgaaca gtacgtccgt tgctcaactc ttctttaata cgttcgttga
    27301 tcagtacgtt cgcatcgacc gctaccgcaa gggttaagac gatacccgcg atacctggca
    27361 tactcagtgt tgcgcctggc aacagcgaca taataccgac gatcaagatc aagttggcaa
    27421 tcagagcact ggtcgcaatc agaccaaact tcttatagaa gatgatcatg aacagaatag
    27481 aaaccagcag accggcaagg caagcctcca gcccctgctc aatgttctgc atacccaggg
    27541 tcgggccaat tgtacgttct tcaacaatct gaatcggcgc gatcaacgca ccggcacgca
    27601 gcagcagtga cagctgacgg gcttcgttcg ggttgttgat gccggtgata cggaagctgt
    27661 tacccagacg agactggatg ttggcgatgt taatcacctc ttcctgtttc accagaaccg
    27721 cacgaccatt tgcatctttc ttaccgctgt ctttgtactc cacaaacagg gttgccatcg
    27781 gtttgccgat gttgtccttg gtgaagttag acataatgtt gccaccagcg ctatcgagcg
    27841 agatgttaac ctgcggctgg ttgtattcgt cctggctgga agtggagtcg gtgatatggt
    27901 caccagtcag aattacgcgt ttgtacagca caaccggctg accttcgcgg gtctgtttca
    27961 cttcagagtc gcccggtacg cgaccggatg ccgcagcggc ctgatcaaca ttggtgttta
    28021 ccagacggaa ttccagcgtt gccgtcgcgc ccagaatctc tttcgcacgc gcagtgtcct
    28081 gaataccagg cagttcaaca acgatacggt cagcaccctg acgctgaacc accggctccg
    28141 ccacgccaag ttggtttaca cggttacgca ggatattaat gttctgctgc accgcatatt
    28201 cacgcgcttc actcagacga gcatcgctca ttaccgcacg cagctggttg ctgccctggc
    28261 tgctaatcac caggtccgga tggcgcttgc tcagatacgc aatggcttca tcacgagctt
    28321 tagcatcgcg gaaagtgatg ctcaggccgt agttgttttc tttacgaaca gtggtatacg
    28381 ggatgccctt ttcgcgcagg tcactgcgta ggctatcgat attttgttcc tggagtttgc
    28441 caagcgcggt gtccatatcc acttccatca ggaagtgaac gccgccacgc aggtcaaggc
    28501 cgagcttcat cggctcagca tgaatagctg ccagccagcg cggcgttgcc ggggcaaggt
    28561 taagcgccac gacgtatttg tcacccataa cgcccattaa tgcttcacga gcgcgcaact
    28621 gggtgtcagt ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc acagacttag
    28681 cagttatttt ttcttcttgt aacgttttct ggacctggat cagcgtttgc tcactggcgg
    28741 cgactccgcg cgcaccagtg atctgaacag ccggatcctc accaaacagg ttgggaagcg
    28801 catacagcag accgatgaca atcaccacga tcagcatgac gtacttccac aaaggataac
    28861 ggtttaacac ggcaattccc ttagggaaaa attttaatta cagcgccttc atggtgcctt
    28921 tcggcaggac ggcagctacg aagtcacgtt taataactac ttcagtggtg tcattcagcg
    28981 cgatagcaat gtagccgttt tccgctactt tggttacgcg accaaccagg ccaccgttcg
    29041 tcagaacttc atcacctttg gcaatggagt ccatcagctt tttgtgttct ttggtgcgct
    29101 tctgctgtgg acgcaggatc atgaaataga aaatcagacc gaataccacc agcatcaaaa
    29161 tcaaagacat cgggctacct tgcgccggtg cacccgttgc cgctaccgca tcagaaataa
    29221 aaaagctcat taaatttccc tcattattaa tattaatcaa cgttcaaagg tggtacttct
    29281 cgcccctgac gctggtaaaa atcagttacg aagctctcta atttaccctc ttcaatagcc
    29341 ttgcgtaaac ccgccatcaa acgctggtag taacgaaggt tatgaatggt gttgagtcgc
    29401 gcgcctaata tttcgttgca acggtcgaga tgatgcaagt aagcgcgtga ataattgcga
    29461 caggtgtagc aatcacactc aggatcgagt gggccagtat cgctcttata tttcgcattg
    29521 cggattttca ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt gcgggttggc
    29581 attacgcagt caaacatatc gataccgcga cgtacgcctt caaccaggtc ttccggttta
    29641 ccaacgccca tcaggtaacg cggtttgtct gccggaattt gcggacacac atgctccaga
    29701 atacggtgca tatctgcttt cggctcaccc acagccagac cgccgacagc gtagccatca
    29761 aagccgatat ctaccagacc tttaacagat atatcacgta aatcttcgta aatactgccc
    29821 tgaattatac caaataacgc atttttgtta ccaagactgt caaaacgctc acggctacgc
    29881 ttcgcccaac gcagagacat ctccatagag cgttttgcgt aatcccagtc agcaggatac
    29941 ggcgtacact catcaaagat catgacgata tccgaaccaa gatcgtactg aatctccatc
    30001 gacttttccg ggtcgaggaa aatcgggtcg ccgttgatcg ggttacggaa gtgaacgccc
    30061 tgttcggtga ttttacgaat atcaccaagg ctgaagacct ggaagccgcc ggagtcggta
    30121 agaatcggtc ctttccactg cataaaatcg tgcagatcgc cgtgcagttt catgatttcc
    30181 tggcccgggc gcagccacag gtggaaagtg ttgccgagga taatttgcgc gccagtggct
    30241 tcaacttctt ccggcgtcat cccttttacg gtgccgtagg tgccaacagg cataaaacaa
    30301 ggcgtttcca ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg accgtcggtg
    30361 gtgtccagtt caaatttcat tttttctcca ctacgtcaga aaaacagtcc aacgtttaaa
    30421 ccagcgccgc ggaattactc cccgacgcgc tcattaattg cctgcggatt gtacgtgata
    30481 aacatcgcat caccgtaact aaaaaagcga tatttctctt ctaccgccgc tttataggca
    30541 ttcatggtgt gttgataacc tgcaaaggcc gaaaccagca taatcagcgt cgactctggc
    30601 aagtggaagt tcgtcaccag cgcatcgacc actttgtact ggaagcccgg atagataaag
    30661 atttgtgtat cgtcaaagaa cggttcgata agatcgtttt ttgccgcctg tgccgcgctt
    30721 tccagcgaac gtactgaagt ggtgccaacc gcaatcaccc ggttaccgcg cgctttcgcc
    30781 gccagtaccg cgtctaccac atcctgcggt acttcagcgt attccgagtg catgatgtga
    30841 tcttcaatgg tgtcgacgcg caccggctgg aaggtgcccg caccaacgtg caacgtcaca
    30901 aacgccatct ccacgccttt ggcgcgtaat ttttccagca aaggctcgtc aaaatgcaga
    30961 ccagcggtcg gggctgcaac cgcgcccggt ttttcgctat aaacggtttg ataaagttcg
    31021 cggtcagcgt cttcgtccgg acgatcgata tacggcggca gcggcatatg gccgatgctg
    31081 ttgaggatat ccagtaccga gcgttcatca ttaaattcga cttcaaacag tgcgccgtgg
    31141 cgcgcggtca ttgttgcgtt gatactttcg tcatcgccca gcaacagttc tgcgccaggt
    31201 tttggcgctt tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag catccgctca
    31261 accagcactt caatcttgcc gccgctggct ttacgcccaa acagacgcgc cgggatcacg
    31321 cgggtattat taaaaaccag aagatcgccg gggttgagct tatcgagtaa atcggtgaaa
    31381 gtaccgtgcg tcagcgcacc cgtcggcccg tccagcgaca gtaaacgaca gctactgcgt
    31441 tcaggcatgg gatagtgggc aatcagggat tcgggcaatt caaaggagaa atcggtaacg
    31501 cgcatgacac tgactcagac taaaataaga gtcgggtagt ctagtgccgg ggcgctctcc
    31561 ctgcaacctt tacccccctt tggatagaat ataatcgatg aattttttag ctcacctgca
    31621 tttagcccat ctcgcggaaa gctcgctttc tggcaattta ctggctgatt tcgtacgcgg
    31681 aaatcctgaa gaaagttttc cgcccgacgt cgtggctggc attcatatgc atcgacgtat
    31741 tgactgacaa tctgccggaa gtccgcgaag cacgggagtg gtttcgtagt gaaacgcgcc
    31801 gcgttgcgcc tattacgctg gatgtcatgt gggatcactt tctttcccgc cactggtcgc
    31861 agctgtcgcc ggactttccg ctacaggaat ttgtctgtta tgcccgcgag caagtgatga
    31921 cgattttgcc ggactcaccg ccacgtttta tcaatctgaa caattacttg tggtcagagc
    31981 agtggctggt gcgctatcgc gatatggatt tcatccagaa cgtgttaaac ggcatggcaa
    32041 gccgccgccc acgtctggat gccctgcgtg actcctggta cgatttagac gctcattatg
    32101 acgccctcga aacccgcttc tggcagtttt atccgcggat gatggcgcag gcgtcacaca
    32161 aggcgttata aaacgttagt tcatccatac cgtagccgaa atggcaggca aagccagaat
    32221 cccgtcagtc agttgcccat gcccttcttt gcattgccat tgcacggcat tgagaaacgg
    32281 tgacgcgggt agcaccactt cactggcctc gccacggttg attgccacca gtacacgttg
    32341 ctgattcagc acgcggacaa ataccactac gttatcttcc gcatacagca cctgacagcc
    32401 gccacgacgc agcgcctgac ttttcttacg cagcacaatc attcgccggt acagcgcgaa
    32461 taacgccgta tcctgctttt ccacctgcca ggggaacggt ttacggcaag acggatcgtt
    32521 tttaccatcc agtcccactt catcaccgta ataaatgcac ggtacaccag gccaggtgaa
    32581 cagccagacc accgccagcg gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg
    32641 cgcagtatcg tggctatcga gctgattaaa catacgtaat tgttgttgat gagaaagccc
    32701 tgcgcggtaa ttatccatcc aggccataca ggtttgggcg tcaatttgct gcggatcata
    32761 agagatgtcg gtattggcaa gaaatcccca caacgggaat gtgaagccac gatagttcat
    32821 ggcagcatct tccacatcgg cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac
    32881 aatatacgct tccgactggg tttctttcgc cgcttcggtg atcccggcaa cgtgctgcat
    32941 attattgcgc gccccacccg cctcccccag catatgcacc acatccagcc gccagccgtc
    33001 catattccac ggcgctttca gccagtggcg gacaatactg tcttccccgc gataaatttc
    33061 attcaccaga ctttccgact gataatccag cttcggcaag ctggcatagc caagccagtc
    33121 gagcgccgtg ccatcatcac taaacaagta ccagtcgcgc cagggcgatt cagagttgtg
    33181 acaagctccc cccgtgccaa gattgtgcct gtcaaaccag gcatgggaat cgccactgtg
    33241 gttaaacacg ccgtccagca ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg
    33301 cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg cgataatcct cggtatcgta
    33361 tttatgtacg ctgggagctt taaacaccgg attgagatac agcgctgtca cgccaagctt
    33421 tttcagatac ggcagttttt cgcttatccc gtccagatcg ccgccataga acgttgatcc
    33481 gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag atgatctctt gtccggctgc
    33541 atgatggtaa tagacatgat cctgttcagc ttcacgagga agactacgcg caaaacgatc
    33601 agggaagatc tgataaaaaa tctgatccgc agcccattgt gggccgatat ccggtacatc
    33661 gacggcaaac tgctccagtc gtgccggcgg cattcggctg aagccctgcg gtgtaaacca
    33721 acgctggcga tcgtgccaca gcagtttgaa actgtaacgc cgccggggtt gtccgctgga
    33781 gagatcaatc gccgcacgcc atgcggtgac gcctggctgc ggctgactgc gctgcttatg
    33841 cattggtact gacatttctt cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg
    33901 gtcttcgccc gtcagccaca gtgtaatgag cagttgatct ttgctttgtt taacaaatgg
    33961 gggcaccggc aggtgccatg catttaacat cataattccc ctgtgatgaa aatctgatca
    34021 ccttgccata gcctaatgca ggatcactca tcatctgaag gatatttggg ggaggaggac
    34081 gggggcggat aactttcgga atagccggat caggatttcc tcctgaaccg gcttacagaa
    34141 cgcaagtatt attggttttc agccagctga cgatcgtggc ggcgtttaaa catccagcca
    34201 atcaacagca caacaatcca cgcgaaaccg acatacagcg agatacgcgt atccgggtga
    34261 taaccaatca acccgataat aaagagcagg aaaatcagcc cgccgatggt cgttgctacc
    34321 ccacccggca ctttaaattt cagcgcctta acttcttctg gcggcaaacg gcgacggaag
    34381 gcgatttgcg acagcaggat cataatccac acccataccg tggcgaaggt tgccagcgaa
    34441 gcgatcacca ggaagacgtt ttccggcatg atgtagttca gatacaccgc aaacagcagc
    34501 gcggtagtca tcaccagcac cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa
    34561 attttcggtg cgctgccctg ctctgccata ccgtggagca tacggcctac gccaaatacg
    34621 tcactgttaa ttgccgacag cgaagcggtc agcacaacaa agttaagaat gctggcggca
    34681 aaggtaatgc ccatatgctg gaacgtcagc acgaacgggc taccggcagt gccaacctga
    34741 ttccacgggt agatagacat aatgacgaac agcgtaccga cgtagaacac cagaatacgc
    34801 atcggcacgg agttaatcgc acgcggaatc gatttctcag gatctttcgc ttcaccggcg
    34861 gtaatcccga taatttcgat cccaccgtaa gcaaacatca ccatttgcaa cgacattacc
    34921 atgccaagcc agccgttact gaagaagccg ccgttgctcc acaggttatg aataccggtc
    34981 ggttgcccgc cgttgccaat cccccagatg atgatgccga aaccggcgac aatcatgatg
    35041 atgatggtgg cgactttaaa gaacgagaac cagaattcca gctcaccgaa gaccttcacg
    35101 ctcatcaggt ttacggcgca aatgatcagc accacgctca gtacccaaat ccagtgcggc
    35161 accgtcggga acaagacacc catatagata ccaaaagcgg tcacatcggc gatggcgaca
    35221 ataaggattt caaagcagta ggtccatccg gtaatgtaac ctgccagcgg gccaaggttt
    35281 tcctgcgcat aacgcgagaa agagctggcg gccgggttat gtaccgacat ttcccccagc
    35341 gcacgcataa tgatatacgc cgcgatacca ccgataatat aggccaacaa cacgctcgga
    35401 ccggccattt tgatggcgtc tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc
    35461 agtgccataa agcgtatatg gcgggtactt agcccacgct ttagcttatt cttactttcc
    35521 atcaattcct aacccatcaa cacaataaaa aaccacgggg catcgcagcc ccgtggttaa
    35581 aacaaatgtt cagtgattta gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc
    35641 cagataatgg ccagaaccac catcaccact gttggcatta accacgccag accttgttcg
    35701 gccagcggta aacgctgcgc ccaggacggt aagatatcgc tgaatgcaga tgctttgatc
    35761 ccgtcgagaa taccaaaaag caggctgata aacatcggcg gagcaatcac gcgggacgaa
    35821 ttatgccacc atgagcgtgt aaaacttaat acaaccagtg cgatacacgg cggataaata
    35881 gcggtcagca ccggtacgga gatctggatc agctggctta agccgaggtt agacaccacc
    35941 atcgagaagc cgccgaggat aaacaccagc gtacgataag agagcggtac gtactgggca
    36001 aagaattctg cacaagcaca agtcaggcca accgccgtga ccagacaggc gatgaagatt
    36061 aacgccgcca gcaggaagct accgccgccg ccaaaggtgt gctgaacgta agcatgcaga
    36121 atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc tgtctgaacc cagacagaac
    36181 agcgccaggt acagcaaagt cagaccaaca cccgccatca ggccagccca gacggtataa
    36241 cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg ccgcgttaac aataacgata
    36301 ccaaacacca ttgcgcccag cgtatccatg gtcagatagc cgttaacgaa gccgttagaa
    36361 aatgcagcgt tttgataagc ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc
    36421 gcgacaacag acaggatgac cagcgcgata attttcagcg gcgcaaggaa gttgcccacg
    36481 gtatccagca gcttgcccgg atagagcgaa accagaataa cgatagcgaa atagaccagg
    36541 ctgtagataa acagcggcag cgcggaatca cccgtcagcg gcgcaatccc cacttcaaag
    36601 gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt
    36661 gccagcagta cgccagcgac tttaccgatt ggcgtgctga ggctgtcaac accgccgcca
    36721 acttttgcca gcgccactac cgttaatacc ggcaggccaa cggcagtaat gaggaagccg
    36781 aatgccgcag tccagacgtg ttcgcctgcc tgtaagccga ccattggagg gaaaataatg
    36841 ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat
    36901 cttaattgat gggtcataaa tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt
    36961 tgtgccaatc cctggggatg atacagcggt atatttaaac aattttagcg ggaaatactc
    37021 ccaaccaggc gcggcgaaga atagtttttc gatttaccgc gttaatgcag tcagtatgac
    37081 agcacctgaa gcgcaattta aacgcttata acgttttaaa gcaaggaggg atctaaaaac
    37141 cagaggtata tagcgataag aaatcaccaa ccagtataaa acactatttc cgtgaaaaag
    37201 acatggctaa tcatgcgaac aaaaaatcac caaccgttgt aaaatctaac gcccgtattt
    37261 tgactgcaaa gaaaataagc cagcatcggc tggcttatgg caataaaaga tgacaaaggc
    37321 ggattaatcg ctgtttttgg caattaaacg ttccgggata acaaaactga aacgtgttcc
    37381 ttttcctact gtactctcaa tattcaggcg actttcgtga tgattcaccg catgtttcac
    37441 gatcgctaac cctaatccgc taccgccggt ttgccgggaa cgcgctttat caacgcgata
    37501 aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg cccggtccgt tatcttcaac
    37561 gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg
    37621 cgtatgattc acggcgttat agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt
    37681 gccagacacc ttgaggccgt tatctatctc aaaggtaaat gtctgttttt tctgactcag
    37741 agtctgagcc tcgcgctcaa caacgcgcag catcatcggc acatcaacct tttcattgag
    37801 caattgcgtc ggcgaggctt caattttcga cagcgttagc aactgcttca ccagcccttc
    37861 catccgctgg gtctgctcgc gcatggtgtg caacgctttt tcgcgtaccg cgccttccag
    37921 cggctgctca tccatcatct ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc
    37981 atggctcacg ttggcaaaaa agttacgccg cgccccttcc agttgatgca tttgcgtgac
    38041 atcacgcgcc accatcaaca actgtttgtg ggtataaggc atgacgcgaa tttccagatg
    38101 ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa tcacgcgttt tcagatattg
    38161 cgtaaactcc gggtaacgca gtaggttaag gatgttctgc ccgttatctt ccggccagcg
    38221 caaaccaaga atttgttgcg ccagaccgtt acaccagaaa ataccgccct cttccgtggt
    38281 cagcaccacc gcgtcgggca gcgactccgc gccgctacga aagcgtttaa tcagattgcc
    38341 cagttcacgg cggcgttttt tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg
    38401 ttcccagcta ccacgccctg gtggcggggt catactgcga tccacccaca gccaccatga
    38461 aaggcgcaat aaattccaga aatgccagat aagcagtcct gttaccgatg ccagcaaaaa
    38521 ccagggcagg taaccaaaaa atgcacccag gatgaaagcc gggaggcagc aaagtagcag
    38581 ctccagcacc agccttttcc acgacagccg ttccagcacg taagatactc cagttaagaa
    38641 atcataagcc ctgctctgcg tccgatgagc aaggcgttaa aagcgggttg aaaaacgata
    38701 tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg
    38761 caggcgacga atgtggacat cgaccgtgcg gtcttccaca tacacgttag ttccccagac
    38821 gtggtttaac agctgctcgc ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag
    38881 cagtttaaat tctgtcggcc ccatctccag cggctcttcg cccgccatca ctcggtgaga
    38941 tgtcgggtcg agacttaatc cctgcatctc aatcacctct tccaccgcca ttggcgaaat
    39001 acggcgcatt accgctttga ttcgcgccac cagctccttc ggcgaaaatg gcttggtgat
    39061 atagtcatcc gcgccggttt caaggccgcg cacgcgatct tcttcttccc ctctggcggt
    39121 caacatcacc actggaatat cccgggtcat cgactcgcgc ttgaggtgct tgatgaactg
    39181 gataccggag ccgccaagta acatccagtc aaggagaatt aaatccggcc aaggttcatt
    39241 cagttgattc acagcactgt cataatcttc cgcttcgacc ggctgaaagc cattttgttc
    39301 gagcacgaag cagaccattt cgcgaattgg agcttcatct tctacgacca gaatacgtct
    39361 cgccatgatt tgccctgttg taataaatag gttgcgatca ttaatgcgac gtcattatgc
    39421 gtcagattta tgacagattt atgaaaagct cgtcgcacat atcttcaggt tattgatttc
    39481 cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta taatcgcgac catgcttttt
    39541 cgccagggaa ccgttatgcg catccttcac acctcagact ggcatctcgg ccagaacttc
    39601 tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg actggctgct ggagacagca
    39661 caaacccatc aggtggatgc gattattgtt gctggtgatg ttttcgatac cggctcgccg
    39721 cccagttacg cccgcacgtt atacaaccgt tttgttgtca atttacagca aactggctgt
    39781 catctggtgg tactggcagg aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat
    39841 atcatggcgt tcctcaatac aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg
    39901 cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca ttccgttttt acgcccgcgt
    39961 gacattattt ccagccaggc ggggcttaac ggtattgaaa aacagcagca tttactggca
    40021 gcgattaccg attattacca acaacactat gccgatgcct gcaaactgcg cggcgatcag
    40081 cctctgccca tcatcgccac gggacattta acgaccgtgg gtgccagtaa aagtgacgcc
    40141 gtgcgtgaca tttatattgg cacgctggac gcgtttccgg cacaaaactt tccaccagcc
    40201 gactacatcg cgctcgggca tattcaccgc gcacagatta ttggcggcat ggaacatgtt
    40261 cgctattgcg gttcccccat tccactgagt tttgatgaat gcggtaagag taaatatgtc
    40321 catctggtga cattttcaaa cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta
    40381 actcaaccca tggcggtgct gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag
    40441 tggcgcgatg tatcgcagga gccacctgtc tggctggata tcgaaatcac tactgatgag
    40501 tatctgcatg atattcagcg caaaatccag gcattaaccg aatcattgcc tgtcgaagta
    40561 ttgctggtac gtcggagtcg tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa
    40621 accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg
    40681 gatgaatcgc agcagcaacg tctgcagcat cttttcacca cgacgttgca taccctcgcc
    40741 ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa aaacctgaac tcattaaaag
    40801 gcgaatggaa gattgatttc acccgcgagc cgttcgccag caacgggctg tttgccatta
    40861 ccggcccaac cggtgcgggg aaaaccaccc tgctggacgc catttgtctg gcgctgtatc
    40921 acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa tgatctcatg acccgcgata
    40981 ccgctgaatg tctggcggag gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct
    41041 ggagccagaa tcgggcgcgt aaccagccgg acggtaattt gcaggtgcct cgcgtagagc
    41101 tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt gaaagataag ctggaactga
    41161 cagcgacgtt aaccgggctg gattacgggc gcttcacccg ttcgatgctg ctttcgcagg
    41221 ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt
    41281 taaccggcac tgaaatctac gggcaaatct cggcgatggt ttttgagcag cacaaatcgg
    41341 cccgcactga gctggagaag ctgcaagcgc aggccagcgg cgtcacgttg ctcacgccgg
    41401 aacaagtgca atcgctgaca gcgagtttgc aggtacttac tgacgaagaa aaacagttaa
    41461 ttaccgcgca gcagcaagaa caacaatcgc taaactggtt aacgcgtcag gacgaattgc
    41521 agcaagaagc cagccgccgt cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag
    41581 cgcaacctca actggcggcg cttagtctgg cacaaccggc acgaaatctt cgtccacact
    41641 gggaacgcat cgcagaacac agcgcggcgc tggcgcatat tcgccagcag attgaagaag
    41701 taaatactcg cttacagagc acaatggcgc ttcgcgcgag cattcgccac cacgcggcga
    41761 agcagtcagc agaattacag cagcagcaac aaagcctgaa tacctggtta caggaacacg
    41821 accgcttccg tcagtggaac aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa
    41881 ccagcgatcg cgagcatctg cggcaatggc agcaacagtt aacccatgct gagcaaaaac
    41941 ttaatgcgct tgcggcgatc acgttgacgt taaccgccga tgaagttgct accgccctgg
    42001 cgcaacatgc cgagcaacgc ccactgcgtc agcgcctggt cgcgctgcat gggcagattg
    42061 ttccccaaca aaaacgtctg gcgcagttaa tggtcactat ccagaatgtc actctggaac
    42121 agacgcaacg taatgtcgca cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc
    42181 aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg catcaaaacg ctggaagccc
    42241 agcgtgcaca gttacaggcg ggtcagcctt gtccactttg tggttccacc agccacccgg
    42301 cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca gtctcgatta ctggcgctgg
    42361 aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat
    42421 taacgaagca gcttcagcgc gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag
    42481 cacttactca acaatggcaa gccgtcacgg ccagcctcaa tatcaccttg cagccacagg
    42541 acgatattca accgtggctg gatgcacaag atgagcacga acgccagctg cggttactca
    42601 gccaacgaca tgaattacaa gggcagattg ccgcgcataa tcagcaaatt atccagtatc
    42661 aacagcaaat tgaacaacgt cagcagcagc tcttaacggc attggcgggt tatgcactga
    42721 cattgccaca ggaagatgaa gaagagagct ggttggcgac acgtcagcaa gaagcgcaga
    42781 gctggcagca acgccagaac gaattaaccg cgctgcaaaa ccgtattcag cagctgacgc
    42841 cgattctgga aacgttgccg caaagtgatg atctcccgca cagcgaagaa actgtggcgc
    42901 tggataactg gcggcaggtt catgaacaat gtctcgcatt acacagccag cagcagacgt
    42961 tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa agcccaagcg cagtttgaca
    43021 ccacgctaca ggccagcgtc tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg
    43081 aacaaacact aacgcagctg gaacagctca agcagaatct ggaaaaccag cgccgtcagg
    43141 cgcaaacgct ggtcactcag acagcagaaa cgctggcaca gcatcaacaa caccgacctg
    43201 acgggttggc tctcactgtg acggtggagc agattcagca agagttagcg caaactcacc
    43261 aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg ccagcagctg aagcaggatg
    43321 cagataaccg tcagcaacaa caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg
    43381 ttgaggactg gggatatctg aattcgctaa taggttccaa agagggcgat aaattccgca
    43441 agtttgccca ggggctgacg ctggataatt tagtccatct cgctaatcag caacttaccc
    43501 ggctgcacgg gcgctatctg ttacagcgca aagccagcga ggcgctggaa gtcgaggttg
    43561 ttgatacctg gcaggcagat gcggtacgcg atacccgtac cctttccggc ggcgaaagtt
    43621 tcctcgttag tctggcgctg gcgctggcgc tttcggatct ggtcagccat aaaacacgta
    43681 ttgactcgct gttccttgat gaaggttttg gcacgctgga tagcgaaacg ctggataccg
    43741 cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac catcggtgtg attagccacg
    43801 tagaagcgat gaaagagcgt attccggtgc agatcaaagt gaaaaagatc aacggcctgg
    43861 gctacagcaa actggaaagt acgtttgcag tgaaataact attcagcagg ataatgaata
    43921 cagaggggcg aattatctct tggccttgct ggtcgttatc ctgcaagcta tcactttatt
    43981 ggctacggtg attggtagcc gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt
    44041 atctttggct ctgggcacgt ttggtttggg gatagccgaa tttggcatta tgagcgtgct
    44101 cacggagctg gcgcataacg taggaatttc gattcctgct gccgggcata tgatctcgta
    44161 ttatgcactg gtggtggtgg tcggtgcgcc aatcatcgca ctcttttcca gccgctactc
    44221 actcaaacat atcttgttgt ttctggtggc gttgtgcgtc attggcaacg ccatgttcac
    44281 gctctcttcg tcttacctga tgctcgccat tggtcggctg gtatccggct ttccgcatgg
    44341 cgcatttttt ggcgtcggag cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac
    44401 cgccgccgtg gcggggatgg tttccgggat gacagtcgcc aatttgctgg gcattccgct
    44461 gggaacgtat ttaagtcagg aatgctggcg ttacaccttt ttattgatcg ctgtttttaa
    44521 tattgcggtg atggcatcgg tctatttttg ggtgccagat attcgcgacg aggcgaaagg
    44581 aaatctgcgc gaacaatttc actttttgcg cagcccggcc ccgtggttaa ttttcgccgc
    44641 cacgatgttt ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc catacatgat
    44701 gtttatttcc ggtttttcgg aaacggcgat gacctttatt atgatgttag ttgggctagg
    44761 gatggtgctg ggaaatatgc taagtggcag gatttcagga cgttattcac cactgcgcat
    44821 tgcagcagtg actgacttta tcattgtact ggcactgctg atgctctttt tctgcggcgg
    44881 catgaaaaca acgtcgctta tttttgcttt tatttgttgc gcgggattat ttgccctttc
    44941 agcacctctg caaatattgt tactgcaaaa tgccaaaggc ggagagttat taggtgccgc
    45001 aggtgggcaa atagcgttta acctcggtag cgccgtcggc gcatattgcg gaggtatgat
    45061 gctgacgctg gggctggcat ataattacgt ggcgctgcct gccgccctgt tttcgtttgc
    45121 tgcgatgtca tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg cggatagtcc
    45181 ggtgctggcg aaaccactgg ggtagattac tgtcacggtg cgcgttgctt attgccggat
    45241 gcggcgtgaa cgccttatcc gccctacgcg gttctggcac attttgtagg cctgataaga
    45301 cgcggcaagc gtcgcatcag gcatgatgcg ccaattgcct acgtttttta ctcttgtggc
    45361 cataaccacg cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt actcaccggc
    45421 gtttcacatt cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt ttgatataaa
    45481 cggtctacat tgctcatccc gccccccagg acaatcacat ccggatcgag aatattcacg
    45541 acatgtgcca gcgattttgc cagccgcagc tcgtagcgac gcaatgccag ttccgctacc
    45601 ggatcgcttt cttcaacctg gcggataatt tcactgcctt tcagcgcatg tccgctcaaa
    45661 cgacgataat ccgtcgcgaa tcccgtgccc gaaataaagg tttcaataca accttgttta
    45721 ccgcaataac aagggacttc ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc
    45781 ggattgtgtc cccactcacc tgccgtgcca ttgccgccga tatgcgtccg cccattgaat
    45841 gccacgcccg cgccgcatcc cgtgccgata atcacggcaa ataccgtctg cgctcccgct
    45901 gccgcgccat ctactgcttc tgaaaccgcc agacagttgg catcatttgc cagccgcact
    45961 tcccgctgca acctcgcgct taagtcttta tcgaatggct gaccgttgag ccaggttgaa
    46021 ttggcattct tcaccacacc ggtgtagggc gaaatcgaac ccggaatgcc catacctacc
    46081 gttccgcgct gccctgtcgc ctgctccgcc atatcaacca acgtggcgat cgtttcaata
    46141 gtctgccggt aatcatcacg cggcgtgggc agacgatggc ggtacaactg ctcccctgca
    46201 tcgcccagtg caatcacttc agttttggtg ccgcctaaat cgatacctat acgcactgta
    46261 ctctccttat ttttttcaat atcaatagcg tagagacgga caaccggatt ggcaatgcaa
    46321 ggccgccgac aattcgttat catgcccgct aaatttaacg acaaggccgt ggaaattatc
    46381 atgctgtggt tcaaaaattt aatggtttac cgtcttagcc gcgagatttc gctgcgtgca
    46441 gaagagatgg aaaaacagct agcctcgatg gcatttaccc catgcggcag ccaggacatg
    46501 gcgaagatgg gctgggttcc tccgatggga tcgcacagcg atgcgttaac gcacgttgcc
    46561 aatggtcaaa ttgttatctg cgcgcgcaaa gaagaaaaaa tcctcccgtc tccggtgatt
    46621 aaacaggcgc tggaagcgaa aatcgccaaa caggaagcgg aacaggcgcg taagctgaag
    46681 aaaaccgaaa aagattcgct gaaagacgaa gtgctgcact ctctgctgcc gcgtgctttc
    46741 agccgtttta gccagacaat gatgtggctc gacacggtta acggtttgat tatggtggac
    46801 tgcgccagtg ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag cctggggtcg
    46861 ttaccggttg taccattgag catggaaaac ccgattgaac tgacgctgac cgaatgggtt
    46921 cgctccggta gtgcggcaca gggcttccag ctgcttgatg aagccgagct gaaatcgttg
    46981 ctggaagatg gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga agagatcacc
    47041 aatcacattg aagccggaaa agtggtgact aaactggcgc tcgactggca gcagcgcatt
    47101 cagtttgtga tgtgcgacga tggttcgctc aagcgtctta agttctgcga cgagctgcac
    47161 gatcaaaacg aagatatcga ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc
    47221 ctgatgactg gtgaactggc agcgttaatt caaaacctga ttgaaggatt aggtggcgaa
    47281 gcacaacgtt aattgctgat tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc
    47341 cggcgtacga agccacacca ggcatataat tattcgctac ggcgagcaat aatttttagc
    47401 gcagcaatat tatgcgtttt acgccgtaac tgatctaatc gcctcatccc tattcgcctg
    47461 tcagcagcac gcagaatatt cactaacccc catttatcgc tggtaaattg ttttgttaac
    47521 agctccgctt ttggggtatc aatatattca cgcaataaat cagaaatgga acaaatatcc
    47581 gtcaggtaag gataccagca agtttcaccg tgaaaattac gcactccagc gttacctgca
    47641 ataaagtctt tcgggtaatc ccagatcacg tttttatcca acgtgatcca ataacgcggt
    47701 aaatcggtac ttccaccatt ttggctgcgc atgggataac gggtacagtg aatttgcagg
    47761 ttgatcgttg gcgtcagtaa gtcatagatc tcccgctgta acttgctcca tggacgttgt
    47821 gtcattgttt ttcctcaagc cggtgacgcg cttcactgcg aattttccgc gttgccccgg
    47881 ttttcactaa atcacgtaac tgcgaaagat cgggttgttc gacaaataag agtaatgatg
    47941 gatcctcttt taaccacgcc gttttcacat cggcagccag ctgtggatta ttaatagcca
    48001 gcgaccgatc ctgaggctca gtcaatgtcg ttaacaacga cgccggggta tgtgcgttgc
    48061 gtagtgccgt ttggcgcaaa tgatcattgc gactttgcag caatgcacgc caccattcat
    48121 catctgattt atcttttatc gtagccagtt ccgcaaaaaa tacctggag
//
LOCUS       DADMFL010000017        47458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.017, whole genome shotgun sequence.
ACCESSION   DADMFL010000017 DADMFL010000000
VERSION     DADMFL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.017"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(2..94)
                     /locus_tag="JNZ27_000980"
     tRNA            complement(2..94)
                     /locus_tag="JNZ27_000980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNZ27_000981"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNZ27_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY8663406.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNZ27_000982"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNZ27_000982"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY8663407.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNZ27_000983"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNZ27_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY8663408.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNZ27_000984"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNZ27_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY8663409.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNZ27_000985"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNZ27_000985"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY8663410.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNZ27_000986"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNZ27_000986"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY8663411.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8018
                     /gene="srlA"
                     /locus_tag="JNZ27_000987"
                     /gene_synonym="gutA"
     CDS             7455..8018
                     /gene="srlA"
                     /locus_tag="JNZ27_000987"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY8663412.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8015..8974
                     /gene="srlE"
                     /locus_tag="JNZ27_000988"
                     /gene_synonym="gutE"
     CDS             8015..8974
                     /gene="srlE"
                     /locus_tag="JNZ27_000988"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY8663413.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8985..9356
                     /gene="srlB"
                     /locus_tag="JNZ27_000989"
                     /gene_synonym="gutB"
     CDS             8985..9356
                     /gene="srlB"
                     /locus_tag="JNZ27_000989"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY8663414.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9360..10139
                     /gene="srlD"
                     /locus_tag="JNZ27_000990"
     CDS             9360..10139
                     /gene="srlD"
                     /locus_tag="JNZ27_000990"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY8663415.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10244..10603
                     /gene="gutM"
                     /locus_tag="JNZ27_000991"
     CDS             10244..10603
                     /gene="gutM"
                     /locus_tag="JNZ27_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY8663416.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10670..11443
                     /gene="srlR"
                     /locus_tag="JNZ27_000992"
                     /gene_synonym="gutR"
     CDS             10670..11443
                     /gene="srlR"
                     /locus_tag="JNZ27_000992"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY8663417.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11436..12401
                     /gene="gutQ"
                     /locus_tag="JNZ27_000993"
     CDS             11436..12401
                     /gene="gutQ"
                     /locus_tag="JNZ27_000993"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY8663418.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNZ27_000994"
     CDS             complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNZ27_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY8663419.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAVCARMLETDVANLHRLAKRLGLKD"
     gene            14099..15538
                     /gene="norV"
                     /locus_tag="JNZ27_000995"
     CDS             14099..15538
                     /gene="norV"
                     /locus_tag="JNZ27_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY8663420.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15535..16668
                     /gene="norW"
                     /locus_tag="JNZ27_000996"
     CDS             15535..16668
                     /gene="norW"
                     /locus_tag="JNZ27_000996"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY8663421.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNZ27_000997"
     CDS             complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNZ27_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY8663422.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDDD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQQPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNZ27_000998"
     CDS             complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNZ27_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY8663423.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19877..20890)
                     /locus_tag="JNZ27_000999"
     CDS             complement(19877..20890)
                     /locus_tag="JNZ27_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8663424.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            21147..22604
                     /gene="ascF"
                     /locus_tag="JNZ27_001000"
     CDS             21147..22604
                     /gene="ascF"
                     /locus_tag="JNZ27_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY8663425.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            22613..24037
                     /locus_tag="JNZ27_001001"
     CDS             22613..24037
                     /locus_tag="JNZ27_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8663426.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24152..24571)
                     /locus_tag="JNZ27_001002"
     CDS             complement(24152..24571)
                     /locus_tag="JNZ27_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8663427.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24568..24879)
                     /locus_tag="JNZ27_001003"
     CDS             complement(24568..24879)
                     /locus_tag="JNZ27_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY8663428.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25052..25795)
                     /locus_tag="JNZ27_001004"
     CDS             complement(25052..25795)
                     /locus_tag="JNZ27_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663429.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(25838..26308)
                     /gene="hycI"
                     /locus_tag="JNZ27_001005"
     CDS             complement(25838..26308)
                     /gene="hycI"
                     /locus_tag="JNZ27_001005"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY8663430.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26301..26711)
                     /locus_tag="JNZ27_001006"
     CDS             complement(26301..26711)
                     /locus_tag="JNZ27_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY8663431.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26708..27475)
                     /gene="hycG"
                     /locus_tag="JNZ27_001007"
     CDS             complement(26708..27475)
                     /gene="hycG"
                     /locus_tag="JNZ27_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY8663432.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27475..28017)
                     /gene="hycF"
                     /locus_tag="JNZ27_001008"
     CDS             complement(27475..28017)
                     /gene="hycF"
                     /locus_tag="JNZ27_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY8663433.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28027..29712)
                     /locus_tag="JNZ27_001009"
     CDS             complement(28027..29712)
                     /locus_tag="JNZ27_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY8663434.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDVNKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29730..30626)
                     /gene="hycD"
                     /locus_tag="JNZ27_001010"
     CDS             complement(29730..30626)
                     /gene="hycD"
                     /locus_tag="JNZ27_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY8663435.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30629..32506)
                     /gene="hycC"
                     /locus_tag="JNZ27_001011"
     CDS             complement(30629..32506)
                     /gene="hycC"
                     /locus_tag="JNZ27_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY8663436.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGIILLGLGLGLGLGLGLGAGVTGIALEQPAL
                     IALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVG
                     LMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAK
                     VYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPL
                     EPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITA
                     HGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRG
                     A"
     gene            complement(32503..33114)
                     /locus_tag="JNZ27_001012"
     CDS             complement(32503..33114)
                     /locus_tag="JNZ27_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8663437.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33239..33700)
                     /gene="hycA"
                     /locus_tag="JNZ27_001013"
     CDS             complement(33239..33700)
                     /gene="hycA"
                     /locus_tag="JNZ27_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY8663438.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33912..34262
                     /gene="hypA"
                     /locus_tag="JNZ27_001014"
     CDS             33912..34262
                     /gene="hypA"
                     /locus_tag="JNZ27_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8663439.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34266..35138
                     /gene="hypB"
                     /locus_tag="JNZ27_001015"
     CDS             34266..35138
                     /gene="hypB"
                     /locus_tag="JNZ27_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY8663440.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35129..35401
                     /gene="hypC"
                     /locus_tag="JNZ27_001016"
     CDS             35129..35401
                     /gene="hypC"
                     /locus_tag="JNZ27_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY8663441.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35401..36522
                     /gene="hypD"
                     /locus_tag="JNZ27_001017"
     CDS             35401..36522
                     /gene="hypD"
                     /locus_tag="JNZ27_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY8663442.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36519..37529
                     /gene="hypE"
                     /locus_tag="JNZ27_001018"
     CDS             36519..37529
                     /gene="hypE"
                     /locus_tag="JNZ27_001018"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY8663443.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37603..39671
                     /gene="flhA"
                     /locus_tag="JNZ27_001019"
                     /pseudo
     CDS             37603..39671
                     /gene="flhA"
                     /locus_tag="JNZ27_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39708..40061)
                     /locus_tag="JNZ27_001020"
     CDS             complement(39708..40061)
                     /locus_tag="JNZ27_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY8663444.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40093..40272)
                     /locus_tag="JNZ27_001021"
     CDS             complement(40093..40272)
                     /locus_tag="JNZ27_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663445.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            40348..42909
                     /gene="mutS"
                     /locus_tag="JNZ27_001022"
     CDS             40348..42909
                     /gene="mutS"
                     /locus_tag="JNZ27_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY8663446.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMEEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43015..43667
                     /gene="pphB"
                     /locus_tag="JNZ27_001023"
                     /pseudo
     CDS             43015..43667
                     /gene="pphB"
                     /locus_tag="JNZ27_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(43718..44515)
                     /locus_tag="JNZ27_001024"
     CDS             complement(43718..44515)
                     /locus_tag="JNZ27_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8663447.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            44681..45589
                     /locus_tag="JNZ27_001025"
     CDS             44681..45589
                     /locus_tag="JNZ27_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8663448.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSNNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            45586..46848
                     /locus_tag="JNZ27_001026"
                     /pseudo
     CDS             45586..46848
                     /locus_tag="JNZ27_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            46845..>47458
                     /locus_tag="JNZ27_001027"
     CDS             46845..>47458
                     /locus_tag="JNZ27_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8663449.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
BASE COUNT        11223 a        12960 c        12362 g        10913 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaacga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gatgcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tacatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaagttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttgcgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggaa tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgctggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctaggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacgctga
     7861 acctgccact tggcccactg gcggtgagtt atctgctggt tggtctggtc accaatttct
     7921 tccgcggctg ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac
     7981 ttgaacaaaa agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg
     8041 aacgggtggc tggggcggcc cgcttgagct ggaagctacg ccgggcaaaa aaatcgtcta
     8101 tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg gcacaactta ctggctggca
     8161 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
     8221 cgactgtggc ggcacattac gctgcggcat ctatccgaaa cgacgtattc ccaccattaa
     8281 tatccactcg acgggcaagt ccggtccgct ggcgcagtac attgtggaag atatttatgt
     8341 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
     8401 cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa caaagcgatg gtttactggc
     8461 gaaggtggga atgggcatgg ggtccgccgt tgccgtgttg tttcagtctg gtcgtgacac
     8521 catcgacact gtattaaaaa ccattctgcc gtttatggca ttcgtctcgg cgctcattgg
     8581 catcattatg gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag
     8641 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccactgc tttcaccttt
     8701 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
     8761 tctcggcaat attccgccgc atctggcttt accggcactg tttgccatca acgcgcaggc
     8821 ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
     8881 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat
     8941 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc
     9001 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
     9061 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
     9121 gcgagttgaa aggtgcactc catcccggtt tgcaattttc actcgggcag catcgctatc
     9181 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
     9241 tgcgcttcga tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg
     9301 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
     9361 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
     9421 gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
     9481 atgtggcaca agaaattaac gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg
     9541 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc
     9601 gcgtggattt gctggtctat agcgccggaa tagcaaaagc agcctttatc agcgacttcc
     9661 agctcggcga ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc
     9721 gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact
     9781 cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg
     9841 gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
     9901 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg
     9961 cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    10021 tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct gctgttctac gccagtccta
    10081 aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    10141 caacagcgga gatccgttaa ggatctccgt gagactatag aatgcctgat gcgctacgct
    10201 catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat
    10261 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc aaatttctcg
    10321 ttttaaccgt gccttcgaca cactatgcca gcaagggagg gttggcgtgg gccgttccag
    10381 cgggcacttt aaaccgcggg tcgtggtcgc catcgcgctg gacgatcagc agtgcatcgt
    10441 cgacaccttg tttatgaaag gactgactgt cttcgcccga ccgcaaaaaa ttcccgcaat
    10501 taccggcatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
    10561 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
    10621 cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
    10681 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
    10741 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
    10801 acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga
    10861 tccgcctatc gatcataaaa cgctcatcaa cacccacaag aaagagctga ttgcagaagc
    10921 tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt
    10981 gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca acagcctgca
    11041 tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
    11101 atttcgcaaa aaatcggcct catttcacgg gcaactggca gagaatgcct tcgagcattt
    11161 caccttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
    11221 gttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt
    11281 gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag
    11341 cgtcgataag ctaattaccg acgcaggtat cgatccggcg tttcgtcagg cgctggaaga
    11401 gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
    11461 gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg
    11521 atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
    11581 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc agtaccggca
    11641 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
    11701 gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta
    11761 ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac
    11821 cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
    11881 tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga
    11941 tggcggtcat gcaggcgcgc ggttttaatg aagaagattt tgcccgctcc cacccagctg
    12001 gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgtcgt gatgacgcta
    12061 tcccacaggt agcgttaacc gccagcgtga tggatgcgat gctggaactc agccgcaccg
    12121 gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg
    12181 gcgatttacg tcgctggctg gtaggcggcg gcgcactcac cacgccagtt aatgaagcga
    12241 tgacgacagg cggcaccaca ttacaggcac aaagtcgcgc catcgacgcc aaagaagttc
    12301 tgatgaaacg caaaatcact gccgcaccag tggtagatga aaacggcaaa ctcaccggcg
    12361 caattaacct gcaggatttc tatcaggccg ggattattta atccttcaat cccagacgtt
    12421 tcgccagccg atgcaggttg gcgacgtcag tttccagcat tcgcgcacag actgcccagt
    12481 tgtgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt
    12541 cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
    12601 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg
    12661 ccagcactac cgcccgatga atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt
    12721 agtgttgcag taaatttcgc gccccggcac ttaataccac gcgggagagc ccctgccgca
    12781 aacgacactg ctcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
    12841 gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caaatcggcg cggaatcgcc
    12901 ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc
    12961 gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct
    13021 tggcctgcaa tgccaacgac aactcgccga tctcatcgag aaacagagta ccgttatccg
    13081 ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct ttcacatgcc
    13141 cgaacaactc actttccgcc acactttccg gcagtgcggc acagttgaga tagaccagcg
    13201 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
    13261 ccgtctcacc gctgatcaga acgttgagat cggacgccgc cacaatctca atctcttttt
    13321 tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg
    13381 gcgtggcatc gcctggcagc atattctggc tttccagttg ttcaatcagc aacgcattgc
    13441 ttaacgctcc cgccgccagc gcggcaatca gccgtaactc ttcgtcgctg aaaacatcga
    13501 actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgt ccgacaaaca
    13561 atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc aaaccgtcat
    13621 agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg cgggcaatcg
    13681 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacatcc tttgccagac
    13741 cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc
    13801 actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc
    13861 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
    13921 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
    13981 tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa gaaactggca
    14041 cgcaatctgc aattagcaag acatcttttt agaacacgct gaataaattg aggttgctat
    14101 gtctattgtg gtgaaaaata acattcattg ggtgggtcaa cgtgactggg aagtgcgtga
    14161 ttttcacggc acggaatata aaacgctgcg cggcagcagc tacaacagct acctcatccg
    14221 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
    14281 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta accatgcaga
    14341 agaggaccac gccggggcgc tgaccgaact gatggcgcaa attcccgata cgccgatcta
    14401 ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
    14461 taatgtggtg aaaactggcg acacgctgga tatcggcaac ggcaaacagc tcatttttgt
    14521 cgaaacacca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
    14581 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
    14641 agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
    14701 gttcagccgc ctggtaacgc cgaaaattac cgagatcctg ggctttaact taccagtcga
    14761 tatgatagcg acctcccacg gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct
    14821 gtacctgaag tgggccgctg attatcagga agacagaatc accattttct acgacaccat
    14881 gtcgaataac acccgcatga tggctgacgc tatcgcccag ggaattgcgg aaaccgaccc
    14941 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
    15001 tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg tgatgatgcc
    15061 gaaaatcgcc gggctggtgg aggagatgac cggattacgc ttccgtaaca aacgcgccag
    15121 tgctttcgga tctcacggct ggagcggcgg tgcggtggat cgcctttcca cgcgcctgca
    15181 ggatgcgggt ttcgaaatgt cgctcagcct gaaagccaaa tggcgaccag accaggacgc
    15241 tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
    15301 gccgcagagc acggtgaata cggtagttaa agaagaaacc tctgccacga cgacggctga
    15361 cctcggccca cgaatgcagt gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
    15421 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctctg
    15481 cccggaatgc tccctcggca aagacgtctt tgacgaactg gcatcggagg caaaatgagc
    15541 aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
    15601 aaacaggacg ccactattcc attaaccctg attgccgccg acagcataga tgagtacaac
    15661 aaacccgacc tcagccatgt tatcagtcag gggcaacgtg ccgacgacct tacccgccag
    15721 acggcgggtg aatttgccgg gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg
    15781 gatatcgatg ccgaagccca tgtggtgaaa agccagaata atcagtggca atacgacaag
    15841 ttagtgctgg caaccggtgc cagcgccttt gtcccgcctg tgcctgggcg tgagttaatg
    15901 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga
    15961 cgcgtgttga ttgtcggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt
    16021 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc attaatgcca
    16081 ccagaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgttg
    16141 aaatctcagt tgcaggggct ggaaaaaacg gattctggca ttctggcaac gctggaccac
    16201 cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc
    16261 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa
    16321 accagtaatg ccgatattta cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg
    16381 ccgttcctgc agccgattca acttagcggg atggtgctgg caaaaaatct tctcggcaat
    16441 aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca aaacgccgga attaccgctg
    16501 catctggcag gcgaaaccca gcgtcaggat ttacgctggc agattaatac cgaacgccag
    16561 ggaatggtgg cgcgcggcgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
    16621 gatcggatga aagaggcatt tgtattgttg aaaacgttgc cggtgtaggt gggctactgt
    16681 gcctaaaatg tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta
    16741 ggccggataa ggcatttacg ccgcatccgg caagggtgtc caaatgcaac acgctttatc
    16801 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    16861 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    16921 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    16981 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17041 catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga
    17101 aagtggcgag atccagttga ttgtccaccc gcggcatcgt caccggatgc gtcactccgt
    17161 cgcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17221 ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac gatgatgcca
    17281 gcggcgcgtt aattccacgc tcaatggccc gcgccagtac gctccagttt tgctgttgca
    17341 cacttgccgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17401 ggtttcgcca cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc
    17461 cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc cacaaagcgc
    17521 cattctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg
    17581 gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt accgtttgcg
    17641 tcggcagatt catttcgctc gcccactggc tggagacata gcccggatgc acatcatgca
    17701 caatgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    17761 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    17821 cgcgcaccag gcagaaggta tttttcagat ccgcgccaag acacagcaca ggcggaacat
    17881 ttttaaagcc cggaggcaaa accagcgcat ccggcacata cccccgcgaa cggcgcagca
    17941 tttcgccgct ttcgcgcacc actgaatcat ccatccgctg cacgatgtcg cggttatgta
    18001 gcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18061 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18121 gcaaatgctg gagcgggttc gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga
    18181 tatcatcaca aagctcaggt acgtattttt tatccaccag cacaatcggc gcagcgggcg
    18241 tggtaagcaa ctggcgcgca gcgtctggta aactttctgc cactggcaac atgactgcca
    18301 gcggtttcgc cgggcgatgt ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg
    18361 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18421 actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc
    18481 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18541 gcgggtcgcg gtactctttg tcacaggccg gacatagcgg aaacgccgcc atcacggtaa
    18601 acgggcggtc gtaaggcatc gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt
    18661 tgataaacgg ataacgataa cgccgctcgc ctggggtatt catttcggca aggcaagcag
    18721 ggcaagtggc ggcatccggg acaatttgcg tattcatggt gccgccggtg ctctggcgga
    18781 tggtgaactc ggtgggctgt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    18841 gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa cgtttccgga tcttccagca
    18901 gccggacttc tacgccatcg tcgtcattac atacatcgcc gtgaagattt aattgctgtg
    18961 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19021 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19081 ccgccctgcc ggacacgaca gcgtcgtatc cggcagtctc aggtcggcga taccgcccgc
    19141 tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19201 ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19261 atcgacacaa atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19321 atggttgcac aggtcgcatt tattggcttc ggctttgtca gcccgtacat tcagacccgc
    19381 gccgctgttg cgaatcacag gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19441 cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19501 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    19561 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    19621 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19681 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19741 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttgctgttat
    19801 ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga
    19861 catgtgatga caatgtttat cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc
    19921 cgctaaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    19981 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20041 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20101 cgcgctcgtg caacgctttg atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20161 cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg
    20221 tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc gacagcgcgt
    20281 ctttataccc ggcaagacgt tcaatgctgg tgggggaatc catcgagccg gtaaggaaag
    20341 caatctcctg atgcccggcg tttatcaact ctgccacggc gttaaagctg gtctgtttat
    20401 gatcgcacca gacgctatgg ctgctattat tgcgcaggcg gcgattaagc accattatcg
    20461 gctgactgtg cgcgtcaatg atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa
    20521 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    20581 cgctgtgttt accatctgcc aatagcaact gtcgcccttt ctcttccgcc attcgcgcgg
    20641 catgaaacag taattcacta aaataaatgc cgtgataaag cgtgttggtc actaccagcc
    20701 ccagcgtctg agtactcttc gccgacagat tgcgggccag caaatttggg cggtaactgc
    20761 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    20821 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    20881 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    20941 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21001 gaacaattaa ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt
    21061 cttaattccg tttcagcatc ggtatttttc cgtcacgtcg actgataaca actacatcta
    21121 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21181 tgatagcggc actgggcggc gttgataaca tctcggcagt cacgcactgt atgacgcgtt
    21241 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21301 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt aattatcggc aatacggttt
    21361 cgcaggcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gcacagcccg
    21421 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatcg
    21481 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    21541 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    21601 tgattggtga cggtgctttc ttcttcctgc cgctaatggt cgcggcatct gccgccatca
    21661 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    21721 ttattgaatt aatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgatcccgg
    21781 tcaccgcggt gaaatacacc tacacggtga tcccggcgct ggtgatgacc tggtgcctgt
    21841 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    21901 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    21961 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22021 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22081 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaagga atggtcatgc
    22141 catcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg ccgtggaaaa
    22201 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22261 ggatttccga accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22321 gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22381 caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg aatccaatga
    22441 gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttt atcctcacac
    22501 tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg cgaaagcatc
    22561 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    22621 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    22681 ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg agcatcgaat
    22741 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    22801 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    22861 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc aggacgatga
    22921 aatcacgccc aatcagcagg gcattgcttt ttatcgtcct gtctttgaag agtgtaaaaa
    22981 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23041 cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac
    23101 ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat
    23161 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23221 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat
    23281 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta
    23341 cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc gggaaaacct
    23401 gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc gcgtatttcg
    23461 cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga aaaacaccgt
    23521 cgattttgtc tctttcagct attacgcctc gcgctgcgcc tcggcggaga tgaacgccaa
    23581 caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    23641 ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg
    23701 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    23761 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    23821 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    23881 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    23941 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24001 aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg atgcggcgtg
    24061 aacgccttat ccggcctaca tagttcgtgc gacatgtagg cccgataagc gcagcgcatc
    24121 gggcaattta gcatttggca ttagtcaaaa atcaaagaaa ccattccggc ttaacgccaa
    24181 atcgggctga aagtgcctta atgtgggata tcgtcaggga tcgttgtcca gacaaaatct
    24241 gactgaccag ggatttagag ccgatttcgt tcttcaaatc cgcataggtc agattatgtt
    24301 gatcaatcag cgtgcgaagt aacgccacgc caacaggcat tgccgcaacg gctttgtcga
    24361 actctgcaaa ctcttggttg ttgttttcat attctgcaat gcggctcgcc agaaagtcga
    24421 taagtggatt ggtgtcatct tcttcaatca aatattcgac cagttccagt gcttcacgat
    24481 aatcatcttc tgagtcacta cctcccagaa aggggactgc attgaccagt tctcttgttg
    24541 ctttaacagc gtgtgcagca ttagcagtca ttctttgttc tctctgtagt aacgtgtcag
    24601 tttgtcatac tcggcgtgat tagttatgtg cttcacaaag aaccgcttat taacaaagtt
    24661 tatgtaggca atcaccccta ggttgttccc acctacatcc agaacgtacc atttgttgcg
    24721 atatttaaaa ttgtcgaggt tgggaaacgc cgttcgcatc tcttctggcg tagaaaaatc
    24781 cgtttcctta ataacccgat acaatgcctg aagagccaga gcgtcgttag gatatttacg
    24841 cgcagactct tcaaatggcg ctttagaaat gatgtgcatt tttaagtttt aacttcctta
    24901 tagtaagtaa acagccacat ctgcgagttc tatccttatg tgttcggaaa ctatagctca
    24961 tcaaatccaa gttcacaata tgtaaacact tgtgcgcgat ataattaccc ccaaaatcct
    25021 catagccact ctgcaaaaag tcagttctgg ttcacccctc aagcacatcc ccctttatca
    25081 agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga
    25141 ggacgagttt acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgt
    25201 caaaccagcc gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc
    25261 agtaaccaga attaaactgc atatccagca attgcgtgtg accattgcct tgcaaatcca
    25321 gcaaatcatc tacgccagtt ttgcttgcgg tgtagaatcc cctgggttca aagacacagg
    25381 gacggccttc gcgcgtgaac gtcataagaa ttaaatgtgc ataaggagcc agaccattac
    25441 cggtacttgg taaccagata accagatcct gaatgccgtt acgatcaaga tcggcgcgat
    25501 agagttgccc tttgcccgct atgtttgcca gcatacccac aggcacagtc caggatttac
    25561 cggcgcggtc tttagcggtt aattgccagt aatcttcttc atttggcatc ttctcaagcg
    25621 tcacgccagc aacgcccact ttcctaaaat gccaggtttg ctcggcatca taaggcatgg
    25681 tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct gtcactcatt ggcagatatt
    25741 cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg
    25801 ttctgatgtt caattccctg aattccctga tggagaatta ttcttcttcc accgctaact
    25861 gcgcaaatcc accatttcct tcccagcctt ccagtcgttg ataaacggtt tctaccgcat
    25921 ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat atccggctga atgccgagga
    25981 aaatcacttc accaatgtct tcttttaact ggtcgataag gtaattcaac ggcatgttat
    26041 gggtagtcat cataaacatc tcggcgatat catccgggtc gatgatgcgg atttcgccgg
    26101 ggtttagccc catatccgtg gcgtcgacaa tcagcagtcg tgtcgggcgc agttcacgga
    26161 tggcgacgat gtcgttttcc ggtgcactac cgccgtcaat caccacccag ttacctttcg
    26221 gcgcggcggc gcacttttcc gccagcagcg ggccagcgcc atcatcgccc atcatgctat
    26281 tgccaacaca gagtaaaacg tcagtcacgc agtctcctca ccatcaaata gatggcgttt
    26341 tcctgatgaa tatcatgcag catacttaac agcattgtac tccattcctg ctgctgcgaa
    26401 gtttgcgctg cccgggcttt atcgaatgcg ttggccagca tcggcacatg gttaatgtcg
    26461 atgacgatct cgccatattt cggtacgcct tccattttgt ggcgggcttc gctgcccgcc
    26521 tccagagtgg caatccatgc cagatattca tcccatgggc aggtgagcgc cgcttccagg
    26581 caatcgataa ccccaaggtg gtgaccaatc gccaagctgt aatagaccac ctgctgcgcc
    26641 tcggcgggcg tggcatcgtt ctcatcaata aatttacggc tcagttgact gaacaccacc
    26701 ttttcactca tcggatacgc gcctcttcaa caacatgatt cagatggctg acaatctcgt
    26761 tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac ctgttcttcg ccctgcccta
    26821 actgtgtaag gtaatcatcg gcaatctgac gaccgtaacg ataacccgcc aggcgacgtg
    26881 cttcgcgatc cactttcacg cgcagcggct gcaccatatc gccatgcagg atctccgccg
    26941 gttgttcatc cagttcaccc ggcccacggg cgtgaatttt ctgctccagc aggccgagcg
    27001 ccattgcaaa gccgtacagc gtggcggcag gcgttggcgg gcagccaggg atataaacat
    27061 ccacagggac aattttatcc gtaccgcccc acacgcagta gagatcgtgt aagatcccgc
    27121 cgctgttacc gcaggcaccg tatgagatac agattttcgg gtccggcgcg gactgccacg
    27181 cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt aaaatatccg
    27241 catgacgcgg tgaaggaacg actttaatgc cgaagcgttc tgcatcgaac agcggagaaa
    27301 gcgtggcgaa aatttcgatt tcgcagccgt tgcagccgcc gcagtccacg cggtaaacat
    27361 aggcagaacg tttgattttt ttcagtaacg acgccttcat gctggcgatg gattcatcca
    27421 ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc taataaattg ctcatcagat
    27481 ggcctctttc atatggcgag tcagttcaat acggtcggac ggcaccaggc atttctggcg
    27541 cttacattcc gggcaagtct caaagctttc gcggtggttt tccgcgcggc tgtcaccatt
    27601 gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgt acggcgaaag gacgattgca
    27661 gacgcggcag ttgcacagcg cgaagcggga ctgttgcagg aagtcttctt tcttccacac
    27721 cgccagttcg tactcttgcg acagtttgat cgccgccgtc gggcaaactt cttcgcagcg
    27781 tccacagaag atgcagcgcc cgagattaaa ctcccacgcc agctctcctg tggcgaggtc
    27841 agtttcaacc gttaaggcgt ttgacgggca ggcattgacg caagccgcgc agccgatgca
    27901 ctgctgcggg ttctgctctg gcttaccacg gaagttttta tcaaccgcaa tcggctccag
    27961 cggataagac gaggtcgccg tgccggtttt gatgactttt ttgataaagg taaacatggc
    28021 gattccttat ttcagcggcg agtttttacg ctcaatgctg taacgctcga gttctttgta
    28081 cggcaccact ttgctcttct tcttacgcac atcgactacg gtcatgcggt cggtacagga
    28141 gtagcaaggg tccaggctac cgataatcag cggcgcatcg gaaacggtat tgccacgcag
    28201 catgtagcgc agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc agcggtacag
    28261 cttctggttg tcgccggtca tgctccagtg gatatcgtcg ccgcgcggcg cttcggcaaa
    28321 gcccagcgcg aaacggtgcg gaatgtaggt aaagccttcc accatcagcg gaccgcccgg
    28381 caggttatcc agaccgtagt cgatcatatt cagcgcggta tagacctcgt tgattcgcac
    28441 tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg acttccattg gcagcaggcc
    28501 ataaccgaca aacgggtgat cggcgcgagt atcgcgggcg agaccgctgg cgcgaaccat
    28561 cgggccaacg ttactgaaat cgcaggcgat ttccgggtcc agacgaccaa tgccgacggt
    28621 gcgttgttcc atgttcggcg tactcagcag cacatccacc agctcctgca cttcacgacg
    28681 catttgttgc gccagctggc gggtctggat catgtcatct ttcagcagat cgtgacgaat
    28741 cccgccgatc aggttcaggc cgtaggtttt acgcgcaccg gtaaggatct ctgccatttt
    28801 catggaggtt tcacgcacgc ggaagaactg cataaagccg gagtcgaagc cggtaaagtg
    28861 acaggcgagg ccgaggttga gcagatgcga gtgcaggcgt tccacctcca gcagaatggc
    28921 gcggatcatc tgcgcgcgtt ctggcacctg aatacccatc gcgttttcca ccgacgtggt
    28981 gtaggcggtg ctgtgggcaa agccgcagat cccgcacaca cggtcagaga ggaaggtcac
    29041 ttcgttataa cccatgcggg tttccgccag tttttccata ccgcgatgga cgtagaacag
    29101 acggtagtcg gcgtcgataa tgttttcgcc atcgacgaac agacggaagt gacccggttc
    29161 gtcagaagtg acgtgcagcg gaccaatcgg cacgacgttg tttttcttgt cgcccagttc
    29221 gttgatgaac tcgtaggttt cagcatcggt agtcggtgcc ggacgctgac gataatccat
    29281 gctgtcttta cgcagcggat aaagttcatc cggccagtca tccggcagac caaccggaat
    29341 caaaccgtac atatcgcgca cttcgcgctc gccccacacc gccgccggaa cgcgcggcgt
    29401 cacggacgga tactccggtt tgttgacgtc aacttcgacg cgaaccgtta cccaacactt
    29461 ggtgcccttc tccatcgaca gcacgtaata aacggcgtaa tgaccattca gtttgcgttc
    29521 gtcgttacca aacagcaccg acagccagcc accctgcttg tagtagagaa actccaccac
    29581 ttccggcaga tagttcacct ttacggtgac agtcagctga tctttggtct gccaggcgtg
    29641 gtccagcacg acgcctggaa atgcctcatt cagcgcggcg agataatgtt gacctaattt
    29701 ttcttcagac atgctcaaac tctctttaat cacgccgcca gcaaggagac gaacgctaaa
    29761 aatgcaaagc caaacccagc ccaggtaatg cgcggagtaa tatcaagacg cagacgggcc
    29821 atactgtttt cgaacagcgc gataaccagg acgccgacca ccagtttgac gatggcaatc
    29881 accagcgcca gcagcagtcc accgacggtg aaggtttcca tttgtcccca cggaataaac
    29941 accccgacga acatctgcaa caccaccagc tgtttcaggc tgatacccca tttcatgacg
    30001 ccaaagccgc taccgctgta ttcggagagc ggaccttcct gcaactcctg ctcggcttcc
    30061 gccaggtcga acggcagttt gcccatttcg ataaaagtgg cgaacgcaca ggcgcagagc
    30121 gccagcacca gcgggatgct ttggctcagc ggccagtgat aaacggtgtc ggtaatgttg
    30181 ctgatgttgg tggaaccggc aacctgtgcg gcaacccaca gaccaagcag caacatcggt
    30241 tcgaccagca cgccaagcat cgcttcacgg ctcgcaccga tagcggtaaa cgggctaccg
    30301 gtatccagac cggaaatggc aaagaagaaa cgcgcgatgg cgaagagata cagtaaggtg
    30361 atcaaatcgc ccagttgcgg cagcggagaa ccgatagtca gcatgacgcc caccatcaca
    30421 tacggcgtca ggcggaacat ccagccagag gcatccgggc caacgctctg gcggcccagc
    30481 agtttgataa tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt atgcagacgg
    30541 gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaataa caccagcgcc
    30601 tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca
    30661 gtaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactacct tcgcactgcc
    30721 agcccggcac cagcgacacc ggattcagcc atttgcgcag tttcagcact ggcgcaaacg
    30781 cctgtttaac cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatggtcgt
    30841 agccgcacac ccaggccgca ccgcgggaac gtgacggcag gcggtcgcct ttgcaaatcg
    30901 ccataatgat gaatggcagc agcgggcagg caatcagcag caatgtgatc attggttgag
    30961 aaacggtggt gttagcaggc tccagaggca gaggtacagc agcagagagc atcggcagca
    31021 gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc
    31081 tcatcaggag cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga
    31141 acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta attgccagac
    31201 ccacagcgag cagcggcccc agcagacggg caacaaacgc gccgctattg ctcagtttga
    31261 agaaggattg atagataacc cattccccgg caaacccgtt cagtggcggc agcgcagcca
    31321 ttgccatcaa cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac
    31381 cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc ccgaggaaca
    31441 gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgacc aggccaagag
    31501 caatcagcgc cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcccca
    31561 gccccagccc cagccccagc cctagcagga taatgccgat attttccagg gtgtggtaag
    31621 ccagcaggcg ctggatgttg tgttccatca acgcatacag accaccgacg aacgcggtga
    31681 tcatgccgag caccagcagc gcgatccccc accacagcgg tgcattaccg cccagcagtg
    31741 acagggttaa catgcccagc aggccaattt tcatgactac cgtagaaaac aacgcggcag
    31801 ctggcgcaga ggcgttagca tgtgcctgcg gcacccagcc gtgcagcgga ataatcccag
    31861 ccagcaggcc aaagccaatc acaccgagca gccagatatc ggaaccgagc agccagatat
    31921 cggaaccgag cggcagctgt tgcatacgca tatccagcag gcgcagatcc agcgtgccat
    31981 aacgctgcca cagcagccag caagcaatca ccagcagcag agtgccaaga cgccccagcg
    32041 caaaccacag tttgccctct ttgctgttgc tggtgaggaa caccgcacac aaggccatga
    32101 cttcggccat taccacgaac atgccgaggt tgctggcgat gacggcgcag acggcggcag
    32161 ccatcaacat attgatctgc aagccgttgc acttcacctg cgcgtggcga tgccagtcaa
    32221 tgttatagag gctgacaaac agaccgcaaa ggccgagggt aatcagccaa atcgcgttaa
    32281 gcggggagat ttgcacatcg tagcttacca gcgacagcgc accgctcacg ccaaccgagc
    32341 cagtcagtac agtgaagccc gcggctgccg tatacagact accaaccgcg ccgccaattc
    32401 cggctatcca gccacttagc gctttttgaa aagaaaagag aaatgccaga acagcggcgg
    32461 cgacaaacca tgccacgccg ctattgatca gggaaattgc gctcatttag cctctccact
    32521 ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg ttaaaagtca gctcacgctt
    32581 acgtttgctg acgcgggcga tatcggtgtt atccaccaga tgcagggctt tagtcgggca
    32641 catccgcaca caggccggac cttgttcatc aaagctacaa aggtcacatt tgacggcgat
    32701 cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca cgcgccggag caggcggtgc
    32761 cggtggcgct ttcggggtat tggcgtttgc cggaatatcc agcggacggc tgccggaaaa
    32821 ttcaattgcg ccaaacgggc aggcgatgcc gcacagtttg cagcttacgc acagactttc
    32881 attcaactgc acggccccat caacgcgggt gatggcgtta accgggcaga ccaccgcgca
    32941 gggtgcatct tcacagtggt gacagagctg cggcgcagat tctttttcat tgagcatcac
    33001 tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac aggcggcctc
    33061 acaggtgtgg cagccgatac agagcgtgga gtcggcaatt acaaaacgat tcaccaggca
    33121 ttcctcaggt gattgtcatt tttgacgaaa acatgccgat gaaatgtcat tttcgacact
    33181 catcgacacg cccatcccca aacaggcgta acgcctgcaa aacgggcaaa gcctcagctc
    33241 atgctgccgg gctttgtccc tttaccagtt ggcttaaatt caccggcaca ttgttttcaa
    33301 tggcggtgta taagctgtcg taaacgccag cgattttttc gagatagtgt tccagaacct
    33361 gttcgcgatc gcggatgttg accttgccat caatcatgcc gtcaatgctc agttgcgcct
    33421 gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg atggtgtggc
    33481 tgttaaagcc atccgccagg gtgacgtaaa tacgaaaatg ttcaaaaagg acgaaacaga
    33541 gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat aacgtgatcc
    33601 cctgaaccag cgaattgcag tagatgtgcc actggtcctg taggcgacga tgccgctgtg
    33661 cgatgtaatc ggctttctcg cttatttccc aaatagtcat tgtcaggtta ctcgtttaac
    33721 agagatgcca gctttaagca ttttttgtac caacttttaa tttattgtta ttaaagagat
    33781 ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt catttcgaca tcatcgacat
    33841 tattcaccgc agggataatc aacactggcg caattattgc ttgtagctgg caatagttaa
    33901 tgggaggcga tatgcacgaa ataaccctct gccaacgggc actggaattg atcgaacagc
    33961 aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt ggcgcatttt
    34021 cttgtgtcga aaccagctct cttgcctttt gttttgatct ggtttgccgc ggcagcgtgg
    34081 cggaaggttg taaactgcac ctcgaagaac aagaggccga atgctggtgt gaaacatgcc
    34141 aacagtatgt gacgctactg acccagcgcg tccgccgctg tccacagtgt catggtgaca
    34201 tgctacagat tgtggcagac gacggtttac agattcggcg gatagaaata gaccaggagt
    34261 gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct gtatatcgag ggtgatgaac
    34321 ataaccctca ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc ccgcagatga
    34381 aaatcaccgg cattaaagcg cctgaattta cccccagcca gactgaagaa ggcgacctgc
    34441 attacggtca tggcgaagcg ggcactcaca caccgggcat gagccagcgt cggatgctgg
    34501 aagtcgaaat tgacgtgctg gacaaaaaca accgtctggc tgaacgcaac cgcgcgcgct
    34561 ttgctgcccg caagcaactg gtgctcaacc tggtttccag ccctggttcc ggtaaaacca
    34621 ccctgctgac ggaaacctta atgcgcctga aagacagcgt tccgtgcgcg gttattgaag
    34681 gcgaccagca aaccgtgaac gatgccgcac gcattcgcgc taccggtaca ccagcgattc
    34741 aggtgaacac cggtaaaggc tgccatcttg acgcacagat gattgccgac gccgcaccgc
    34801 gtctgccact ggacgataac ggtattctgt ttatcgaaaa cgttggcaac ctcgtatgcc
    34861 cggccagctt cgatctcggt gaaaaacaca aagtagcggt gctttccgtt accgaaggtg
    34921 aagacaaacc gctgaaatat ccgcatatgt ttgccgccgc ctcgctgatg ctgctcaaca
    34981 aagttgacct gttgccgtat ctcaactttg acgttgagaa gtgcatcgcc tgcgcccgcg
    35041 aagtcaatcc agaaattgaa atcatcctta tttccgccac cagcggcgaa gggatggacc
    35101 agtggctgaa ctggctggag acacagcgat gtgcataggc gttcccggcc agatccgcac
    35161 cattgacggc aaccaggcga aagtcgacgt ctgcggcatt cagcgcgatg tcgatttaac
    35221 gttagtcggc agctgcgatg aaaacggtca gccgcgcgtg ggccagtggg tactggttca
    35281 cgttggcttt gccatgagcg taattaatga agccgaagca cgcgacactc tcgacgcctt
    35341 acaaaacatg tttgacgttg agccggatgt cggtgcgctg ttgtatggcg aggaaaaata
    35401 atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat tgagcatctg
    35461 cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc ctctgcggat tatggaagtg
    35521 tgtggcggtc atacccacgc catttttaaa ttcggcctcg accagttact gcctgaaaac
    35581 gttgagttta tccacggtcc gggttgcccg gtgtgcgtac tgccgatggg tagaatcgac
    35641 acctgcgtgg agattgccag ccatccggaa gtcatcttct gtacctttgg cgacgccatg
    35701 cgcgtaccag ggaaacaggg atcgctgttg caggcaaaag cacgcggtgc cgatgtgcgc
    35761 atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac ccgcaaagtg
    35821 gtgttcttcg gcttaggttt tgaaaccacc atgccgacca ccgccatcac tctgcaacag
    35881 gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc agcacattac gcttatcccg
    35941 acgctgcgca gtttgctgga acagccggat aacggtatcg acgcgttcct cgcgccgggc
    36001 cacgttagta tggttatcgg cactgatgcc tataatttta tcgccagcga ttttcatcgt
    36061 ccgctggtgg tcgctggttt cgaacccctt gatctactgc aaggcgtggt catgctggtg
    36121 gagcagaaaa tagcggccca cagcaaggta gagaatcagt atcgtcgggt ggtaccggat
    36181 gccggtaacc tgctggcgca acaggcgatt gccgatgtgt tctgtgtcaa cggcgacagc
    36241 gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc acctgacgcc ggattatcaa
    36301 cgattcgatg ccgaagcaca tttccgcccg gcaccgcagc aggtctgcga tgacccgcgc
    36361 gcgcgttgtg gtgaggtatt aacgggcaaa tgtaagccgc atcaatgccc gctgtttggt
    36421 aacacctgta atcctcaaac cgcatttggt gcactgatgg tctcctccga aggagcgtgc
    36481 gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt gaataatatc caactcgccc
    36541 acggcagcgg cggccaggcg atgcagcaat taatcaacag cctgtttatg gaagcctttg
    36601 ccaacccgtg gctggcagag caggaagatc aggcacgtct tgatctggcg cagctggtag
    36661 cggaaggcga ccgtctggcg ttctccaccg acagttacgt cattgacccg ctgttctttc
    36721 ctggcggtaa tatcggcaag ctggcgattt gcggcaccgc gaatgacgtt gcggtcagtg
    36781 gcgctattcc gcgctatctc tcctgtggct ttatcctcga agaaggattg ccgatggaga
    36841 cactgaaagc cgcagtgacc agcatggcag aaaccgcccg cacggcaggc attgccatcg
    36901 ttactggcga tactaaagtg gtgcagcgcg gcgcggcaga taaactgttt atcaacaccg
    36961 ctggcatggg cgcaattccg gcgaatattc actggggcgc acagacgcta accgcaggcg
    37021 atgtattgct ggttagcggt acactcggcg accacggggc gactatcctt aacctgcgtg
    37081 agcagctggg gctggatggc gaactggtca gcgactgcgc ggtgctgacg ccgcttattc
    37141 agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt ggtggtgtaa
    37201 acgcggtggt tcatgagttc gcggcagcct gcggttgtgg tattgaaatt tctgaatcag
    37261 cgctgccggt taaacctgcc gtgcgcggcg tttgcgaatt gctgggactg gacgccctga
    37321 actttgccaa cgaaggtaaa ctggtgatcg ccgttgaacg caacgcggca gagcaagtgc
    37381 tggcagcgtt acattcccat ccactgggga aagacgcggc gctgattggt gaagtggtgg
    37441 aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa acgaaccctc gatttaccac
    37501 acgccgaacc gcttccgcgt atatgctaat aaaattctaa atctcctata gttagtcaat
    37561 gaccttttgc accgctttgc ggtgctttcc tggaagaaca aaatgtcata tacaccgatg
    37621 agtgatctcg gacagcaagg gttgttcgac atcactcgga cactattgca gcagcccgat
    37681 ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc gttctgcgct cgccgacaac
    37741 gcggctattg tgttgtggca agcgcagact caacgtgcgt cttattacgc gtcgcgtgaa
    37801 aaagacaccc ccattaaata tgaagacgaa actgttctgg cacacggtcc ggtacgcagc
    37861 attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga aacctggccg
    37921 caggtgggct ataccccaaa tttggtcact attgcctgat gccactggcg gcgggagggc
    37981 atatttttgg tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt
    38041 tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc
    38101 gcgtcgtcaa caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc
    38161 tggttgccat taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag
    38221 tcgccaaaga aatccattac tatttcgaca ttgacgatat cagcattgtc ttacgcagcc
    38281 accgtaaaaa caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc
    38341 acgaacagag cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag
    38401 agatgctgct gatcaatctc tacgagcggg acgatttagc cccctatgaa cgcatgttgt
    38461 tcgacacctg gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca
    38521 ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc
    38581 tgaatttgct gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct
    38641 atcaggaaat ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc
    38701 agctcaacaa tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca
    38761 gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg
    38821 aaactggcac gggtaaagag ctgattgccc gtgcgatcca taatctcagt gggcgtaata
    38881 atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc
    38941 tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg
    39001 aactggcgga taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac
    39061 agccgaagtt gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa
    39121 tcattcagac ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg
    39181 ccgaccgaga gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc
    39241 cgccactacg cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa
    39301 ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga
    39361 gcaacatgga gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcagtat
    39421 tgctaacacg cggcaacgtg ctgcagctgt cattgccaga tattgcttta ccggagcctg
    39481 aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg
    39541 tgcgcgtgct gaaagaaacc aacagcgtgg ttgccgggcc taaaggcgct gcgcaacgtc
    39601 tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg
    39661 cattgattta actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt
    39721 tttttcggac gatatttttc cggcagttct ggcaccggac gcttgtcatc gatgagatga
    39781 cgcacggtta agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt
    39841 ttcatctctt cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt
    39901 ttctcttcgc cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg
    39961 taatgctccg gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt
    40021 ttaatcgtca gtttttcacg agagatacgc ttgccggaca tgctgcctcc acctcattaa
    40081 gatgcattta tattacatct taatcttaaa gggcactatg actctaaaga agaagggttc
    40141 gccaatcgat acaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag
    40201 catctttccc ggaaccagca tcaagaactc gccgttcgct tcttcccctg aaatgattaa
    40261 ctccggtatc atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt
    40321 taatatcagg gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg
    40381 cccatgatgc agcagtatct caagctgaaa gcccagcatc ccgagatcct gctgttttac
    40441 cggatgggtg atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg
    40501 gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt
    40561 ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc
    40621 atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg
    40681 cgtatcgtta cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac
    40741 ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc
    40801 tccgggcgtt ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa
    40861 cgcactaatc cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa
    40921 ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag
    40981 cagttgaatc tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg
    41041 cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc
    41101 ctgccgcata ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc
    41161 gcgacacgtc gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg
    41221 gcttctgtgc tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg
    41281 catatgccag tgcgcgatac tcgcgtgttg cttgagcgcc agcaaactat tggcgcattg
    41341 caggatttca ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt
    41401 ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct
    41461 ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag
    41521 gcgctacgtg agaagatgga agagtttgcc gagctgcgcg atctgctgga gcgagcaatc
    41581 atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa
    41641 gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa
    41701 gtccgcgagc gtgaacgtac cggcctggac acgctaaaag ttggctttaa tgcggtgcac
    41761 ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt
    41821 cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat
    41881 aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagctg
    41941 ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa
    42001 ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc
    42061 ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta
    42121 ctgaatgagc catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatt
    42181 attaccggcc cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg
    42241 ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat
    42301 cgcatcttta cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg
    42361 gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta
    42421 atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc
    42481 gcagaaaatc tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag
    42541 ctgacccagc taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag
    42601 cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac
    42661 ggtctggcgg ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa
    42721 aaactgcgtg agctggaaag catttcgccg aacgccgccg cgacgcaagt ggatggtacg
    42781 cagatgtctt tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc actggaaaat
    42841 ctcgacccgg attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt
    42901 ctggtgtaat aacaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg
    42961 aatcaaataa aaataccctg tataatagga aagcttattt tacagggtaa aaccatgcca
    43021 tctacacgct atcaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat
    43081 attcatggtg aatatcagtt attacaatcc cgcttacatc aactctcttt tttccccgaa
    43141 accgacttac ttatttctgt cggcgataat attgatcgtg ggccggagag tcttgacgtc
    43201 ctgcgcctgc taaaccagcc ctggtttatc tccgttaaag gcaaccacga agcaatggcg
    43261 ctggatgcgt tcgcgaccgg cgatggcaat atgtggcttg ccagcggtgg tgactagttt
    43321 ttcgatttaa atgattgcaa caagaggcaa tagatctgtt gctgaaattc catcaccttc
    43381 cacatattat tgaaatcact aacgacataa taaaatatgt catcgcacat gcagattatc
    43441 cgggtgatga atatctcttt ggcaaagaaa tagcggagag cgaattactc tggcctgttt
    43501 atcgtgtgca gaaatcgctt aatggcgagt tacaacaaat aaacggcgct gattatttta
    43561 tatttggaca tatgatgttt gataacattc agacgttcgc taaccagatt tatattgata
    43621 ccggatcgcc gaaaagtggg cggctgtcat tttataaaat aaggtagtct catgcttctt
    43681 ctgtgaagca tgagtaacca gatgttattg caggccatta ttcatttttc gctaccagca
    43741 aagagagatc ctgcttcgcc agcgcgcgac tggcactctc cggcaaaccg tcgtctgtaa
    43801 taatctgatc aaactcgctt aatggtaacg ccagccatgt cgccacctga ccatatttcg
    43861 tcgcatcaca gaccaaaact cgctggcggc tagcactggc aatcgcccgt ttcaccgtga
    43921 ctttatcttc cgctggcgta gaaatccccc gcacactcca tgacgatgca gaaataaaag
    43981 cctgatcaat catcaggctg cgcagcatgg tcgcagcggc ttccccgaca caggaacggt
    44041 tttcccgaca cactgcaccg ccagtgtgaa taattgtgca attactgttg tcgagcaagt
    44101 agttcgcaat aacgaaatcg tttgtgacca cagtcagtga ctccatgtga atcagatgct
    44161 gtgctatcgc taacgtggtc gttcccgcat ccagatagat acaacttccc ggctgaacaa
    44221 gacttgccgc cagcttgcca atagccgctt tttgcgtcat tgccagcgca gtttttacct
    44281 gatgagaagg ttcatgcgcc acgcgtcccg gagactggac gcctccggac accagcacaa
    44341 cggctccctg ctgctccagt ttttgtaaat cccgacgaat ggtcatatgt gacacattaa
    44401 ttctgtccgt tagttcagca atactgacaa tgcctttttc agctaccatc tcaaggatga
    44461 tttggcgacg ctctacgggt atcaactttt gctccttcct ttgtcctgct gacattctac
    44521 gctatttgcc tgcgaaacgt gcgcggcgca actaacgctt agttcacata aaataacaca
    44581 aaatgttaat ttatgtgaat cagctcacca tatcgttatc tcccagcgct tatattcaca
    44641 atgtcaaaca aaatatcact taaattaaca aggagagcag atgaaaacgg gatctgagtt
    44701 tcatgtcggt atcgttggct tagggtcaat gggaatggga gcagcactgt catgtgtccg
    44761 cgcaggtttt tctacctggg gcgcagacct gaacagcaat gcctgcgcta cgttgaaaga
    44821 ggcaggtgct tgcggggttt ctaataacgc cgcgacgttt gccgaaaaac tggacgctct
    44881 gctggtgctg gtggtcaatg cggcccaggt taaacaggtg ctgtttggtg aaacaggcgt
    44941 tgcacaacat ctgaaacccg gtacggcagt aatggtttct tccactatcg ctagtgctga
    45001 tgcgcaagaa attgctaccg ctctggctgg attcgatctg gaaatgctgg atgcgccagt
    45061 ttctggtggt gcagtaaaag ccgctaacgg tgaaatgact gtcatggcct ccggtagcga
    45121 tattgccttt gaacgactgg cacccgtgct ggaagccgtt gccggaaaag tttatcgcat
    45181 aggtgcagaa ccgggactag gttcgaccgt aaaaattatt caccagttgt tagcgggcgt
    45241 acatattgct gccggagccg aagcgatggc acttgcagcc cgtgcgggga tcccgctgga
    45301 tgtgatgtat gacgtcgtga ccaatgccgc cggaaattcc tggatgttcg aaaaccggat
    45361 gcgtcatgtg gtggatggcg attacacccc gcattcagcc gtcgatattt ttgttaagga
    45421 tcttggtctg gttgccgata cagccaaagc cctgcacttc ccgctgccat tggcctcaac
    45481 agcattgaat atgttcacca gcgccagtaa cgcgggttac gggaaagaag acgatagcgc
    45541 agttatcaag attttctctg gcatcactct accgggagcg aaatcatgat caagattggc
    45601 gttatcgccg atgattttac cggcgcgacg gatatcgcca gttttctggt ggaaaacggt
    45661 ctaccaacgg tacaaattaa cggtgttcca acaggtaaaa tgccggaagc aatcgacgca
    45721 ctggtgatca gcctgaaaac gcgctcctgt ccggtggttg aagccacaca gcaatcgctg
    45781 gcggctctga gctggttgca acagcaaggt tgcaaacaga tctatttcaa atacagctct
    45841 actttcgaca gtacggcgaa aggtaatatt ggcccggtta ccgatgcatt aatggatgct
    45901 ctcgacacgc cgtttacggt cttctctccg gccctgccgg tcaacggacg tacggtttat
    45961 caggggtatt tgttcgtaat gaatcaactg ctggccgaat ccgggatgcg ccatcacccg
    46021 gtaaatccca tgaccaacag ctatcttccc cgtctggttg aagcgcaatc cacagggcgc
    46081 tgcggcgtcg tttcggcaca tgttttcgaa caaggtgtgg atgccgttcg tcaagagctg
    46141 gctcgcttac agcaagaggg ctaccgctac gcggtgcttg atgcgctgac cgaacaccat
    46201 ctggaaattc agggagaagc cttgcgcgat gccccactgg taacgggcgg ttctggtctg
    46261 gcgattggcc tggcccggca gtgggcgcaa gaaaacggta accaggctcg cgaagcaggg
    46321 cgtccgctcg ctgggcgcgg cgtagtgctc tccggttcat gctctcaaat gaccaaccgc
    46381 taggtggcac attaccgtca aattgcacca gcccgtgaag ttgatgtggc acgctgcctc
    46441 tcaaccgaaa ctctggccgc ttatgcactc gaactggcag agtgggttct gggccaggaa
    46501 agtgtacttg ctccactggt ttttgccacc gccagcactg acgcattggc agcaattcaa
    46561 cagcaatacg gtgcacaaaa agccagtcag gcagtagaaa ctctgttttc tcaactagcg
    46621 gcgcggttag cagcggaagg catgacacgc tttattgtcg caggtggtga gacctccggc
    46681 gtagtcacac agagcctggg cataaaaggg tttcatattg gcccaaccat ttcccccggc
    46741 gtgccgtggg taaacgcact ggataagcct gtctcactcg cccttaaatc tggcaacttc
    46801 ggtgatgaag cctttttttc acgagcccaa agagagtttt tatcatgagc gatttcgcaa
    46861 aagtagagca gtctttgcga gaggagatga cgcggattgc cagttcattc tttcagcgcg
    46921 gctatgcaac cggttcggct ggcaatctgt cgctgctttt acctgacggg aatttactgg
    46981 cgacaccgac aggttcatgc ctgggcaatc tcgatccgca gcggctttcc aaagtcgccg
    47041 cggatggcga atggttaagt ggtgacaaac cctcgaaaga ggtgctcttt catctggcgc
    47101 tgtatcgcaa caatccgcgc tgtaaagcgg tggtgcattt gcacagcaca tggtcgacgg
    47161 cgctttcctg cctgcaaggg ctggacagca gcaacgttat tcgtccgttc acaccatacg
    47221 tggtgatgcg gatgggaaat gtcccgctgg tgccctatta ccgaccgggc gataaacgca
    47281 tcgcacagga tctggcggaa ctggcagcag acaatcaggc ttttttactg gcaaatcatg
    47341 gcccagtggt ttgcggtgaa agcctgcaag aagccgccaa caatatggaa gagctggagg
    47401 aaacggcaaa gctgattttt attctcggtg accgcccgat ccgttatctg accgcagg
//
LOCUS       DADMFL010000018        45453 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.018, whole genome shotgun sequence.
ACCESSION   DADMFL010000018 DADMFL010000000
VERSION     DADMFL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.018"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..66
                     /locus_tag="JNZ27_001028"
     CDS             <1..66
                     /locus_tag="JNZ27_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY8663450.1"
                     /translation="TFSGHTVNSSDMATLKIIGDD"
     gene            92..805
                     /gene="msyB"
                     /locus_tag="JNZ27_001029"
     CDS             92..805
                     /gene="msyB"
                     /locus_tag="JNZ27_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY8663451.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGASHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            954..1520
                     /gene="satP"
                     /locus_tag="JNZ27_001030"
     CDS             954..1520
                     /gene="satP"
                     /locus_tag="JNZ27_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY8663452.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1555..2142)
                     /gene="mog"
                     /locus_tag="JNZ27_001031"
     CDS             complement(1555..2142)
                     /gene="mog"
                     /locus_tag="JNZ27_001031"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY8663453.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTAFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2257..3210)
                     /gene="tal"
                     /locus_tag="JNZ27_001032"
     CDS             complement(2257..3210)
                     /gene="tal"
                     /locus_tag="JNZ27_001032"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8663454.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMVGDLL"
     gene            3489..4979
                     /locus_tag="JNZ27_001033"
                     /pseudo
     CDS             3489..4979
                     /locus_tag="JNZ27_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            5049..5825
                     /gene="yaaA"
                     /locus_tag="JNZ27_001034"
     CDS             5049..5825
                     /gene="yaaA"
                     /locus_tag="JNZ27_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY8663455.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(5978..6274)
                     /locus_tag="JNZ27_001035"
     CDS             complement(5978..6274)
                     /locus_tag="JNZ27_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8663456.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(6488..7774)
                     /gene="thrC"
                     /locus_tag="JNZ27_001036"
     CDS             complement(6488..7774)
                     /gene="thrC"
                     /locus_tag="JNZ27_001036"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY8663457.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(7775..8707)
                     /gene="thrB"
                     /locus_tag="JNZ27_001037"
     CDS             complement(7775..8707)
                     /gene="thrB"
                     /locus_tag="JNZ27_001037"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY8663458.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(8709..11171)
                     /gene="thrA"
                     /locus_tag="JNZ27_001038"
     CDS             complement(8709..11171)
                     /gene="thrA"
                     /locus_tag="JNZ27_001038"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY8663459.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAISRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(11252..11317)
                     /gene="thrL"
                     /locus_tag="JNZ27_001039"
     CDS             complement(11252..11317)
                     /gene="thrL"
                     /locus_tag="JNZ27_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY8663460.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11531..12217)
                     /locus_tag="JNZ27_001040"
     CDS             complement(11531..12217)
                     /locus_tag="JNZ27_001040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8663461.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(12617..12757)
                     /locus_tag="JNZ27_001041"
     CDS             complement(12617..12757)
                     /locus_tag="JNZ27_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663462.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            12853..13569
                     /gene="arcA"
                     /locus_tag="JNZ27_001042"
     CDS             12853..13569
                     /gene="arcA"
                     /locus_tag="JNZ27_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY8663463.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(13628..14980)
                     /gene="creD"
                     /locus_tag="JNZ27_001043"
     CDS             complement(13628..14980)
                     /gene="creD"
                     /locus_tag="JNZ27_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY8663464.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(15038..16462)
                     /gene="creC"
                     /locus_tag="JNZ27_001044"
     CDS             complement(15038..16462)
                     /gene="creC"
                     /locus_tag="JNZ27_001044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY8663465.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(16462..17151)
                     /gene="creB"
                     /locus_tag="JNZ27_001045"
     CDS             complement(16462..17151)
                     /gene="creB"
                     /locus_tag="JNZ27_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY8663466.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17164..17637)
                     /gene="creA"
                     /locus_tag="JNZ27_001046"
     CDS             complement(17164..17637)
                     /gene="creA"
                     /locus_tag="JNZ27_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY8663467.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            17847..18716
                     /gene="robA"
                     /locus_tag="JNZ27_001047"
     CDS             17847..18716
                     /gene="robA"
                     /locus_tag="JNZ27_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY8663468.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(18713..19360)
                     /gene="gpmB"
                     /locus_tag="JNZ27_001048"
     CDS             complement(18713..19360)
                     /gene="gpmB"
                     /locus_tag="JNZ27_001048"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY8663469.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19412..19933
                     /gene="yjjX"
                     /locus_tag="JNZ27_001049"
     CDS             19412..19933
                     /gene="yjjX"
                     /locus_tag="JNZ27_001049"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY8663470.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(20018..20344)
                     /gene="trpR"
                     /locus_tag="JNZ27_001050"
     CDS             complement(20018..20344)
                     /gene="trpR"
                     /locus_tag="JNZ27_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY8663471.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(20434..22371)
                     /gene="sltY"
                     /locus_tag="JNZ27_001051"
     CDS             complement(20434..22371)
                     /gene="sltY"
                     /locus_tag="JNZ27_001051"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY8663472.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22582..24249
                     /gene="ettA"
                     /locus_tag="JNZ27_001052"
     CDS             22582..24249
                     /gene="ettA"
                     /locus_tag="JNZ27_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY8663473.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(24557..25789)
                     /gene="nadR"
                     /locus_tag="JNZ27_001053"
     CDS             complement(24557..25789)
                     /gene="nadR"
                     /locus_tag="JNZ27_001053"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY8663474.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(25810..27192)
                     /gene="radA"
                     /locus_tag="JNZ27_001054"
     CDS             complement(25810..27192)
                     /gene="radA"
                     /locus_tag="JNZ27_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY8663475.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(27241..28209)
                     /gene="serB"
                     /locus_tag="JNZ27_001055"
     CDS             complement(27241..28209)
                     /gene="serB"
                     /locus_tag="JNZ27_001055"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY8663476.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            28315..28959
                     /locus_tag="JNZ27_001056"
     CDS             28315..28959
                     /locus_tag="JNZ27_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY8663477.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            28987..30003
                     /gene="lplA"
                     /locus_tag="JNZ27_001057"
                     /pseudo
     CDS             28987..30003
                     /gene="lplA"
                     /locus_tag="JNZ27_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            30035..30298
                     /locus_tag="JNZ27_001058"
     CDS             30035..30298
                     /locus_tag="JNZ27_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8663478.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMSGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(30459..31178)
                     /gene="deoD"
                     /locus_tag="JNZ27_001059"
     CDS             complement(30459..31178)
                     /gene="deoD"
                     /locus_tag="JNZ27_001059"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY8663479.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(31235..32458)
                     /gene="deoB"
                     /locus_tag="JNZ27_001060"
     CDS             complement(31235..32458)
                     /gene="deoB"
                     /locus_tag="JNZ27_001060"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8663480.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(32510..33832)
                     /gene="deoA"
                     /locus_tag="JNZ27_001061"
     CDS             complement(32510..33832)
                     /gene="deoA"
                     /locus_tag="JNZ27_001061"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY8663481.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(33959..34738)
                     /gene="deoC"
                     /locus_tag="JNZ27_001062"
     CDS             complement(33959..34738)
                     /gene="deoC"
                     /locus_tag="JNZ27_001062"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY8663482.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            34996..36537
                     /locus_tag="JNZ27_001063"
     CDS             34996..36537
                     /locus_tag="JNZ27_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY8663483.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            36509..37372
                     /gene="yjjW"
                     /locus_tag="JNZ27_001064"
     CDS             36509..37372
                     /gene="yjjW"
                     /locus_tag="JNZ27_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY8663484.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHVVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(37586..38368)
                     /locus_tag="JNZ27_001065"
     CDS             complement(37586..38368)
                     /locus_tag="JNZ27_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8663485.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(38365..39438)
                     /locus_tag="JNZ27_001066"
     CDS             complement(38365..39438)
                     /locus_tag="JNZ27_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY8663486.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPTAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(39560..39721)
                     /locus_tag="JNZ27_001067"
     CDS             complement(39560..39721)
                     /locus_tag="JNZ27_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY8663487.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(39848..40453)
                     /gene="osmY"
                     /locus_tag="JNZ27_001068"
     CDS             complement(39848..40453)
                     /gene="osmY"
                     /locus_tag="JNZ27_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY8663488.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(40846..42435)
                     /gene="prfC"
                     /locus_tag="JNZ27_001069"
     CDS             complement(40846..42435)
                     /gene="prfC"
                     /locus_tag="JNZ27_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY8663489.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(42526..43203)
                     /gene="yjjG"
                     /locus_tag="JNZ27_001070"
     CDS             complement(42526..43203)
                     /gene="yjjG"
                     /locus_tag="JNZ27_001070"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY8663490.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(43218..43664)
                     /gene="rimI"
                     /locus_tag="JNZ27_001071"
     CDS             complement(43218..43664)
                     /gene="rimI"
                     /locus_tag="JNZ27_001071"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8663491.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(43633..44046)
                     /gene="holD"
                     /locus_tag="JNZ27_001072"
     CDS             complement(43633..44046)
                     /gene="holD"
                     /locus_tag="JNZ27_001072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY8663492.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            44149..45180
                     /gene="rsmC"
                     /locus_tag="JNZ27_001073"
     CDS             44149..45180
                     /gene="rsmC"
                     /locus_tag="JNZ27_001073"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY8663493.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        10701 a        12387 c        11529 g        10836 t
ORIGIN      
        1 accttttccg gtcatacggt gaactcatcg gatatggcca cgctgaaaat tatcggcgac
       61 gactaacgcc agaatataga agccacaaaa aatgaatgtt aattacctga atgattcaga
      121 tctggatttt cttcagcatt gtagtgagga acagttggca aatttcgccc gattgctcac
      181 ccataatgaa aaaggcaaaa ctcgcctctc cagcgtactg atgcgcaacg aactgtttaa
      241 atcgatggaa gggcatcccg agcaacatcg ccgcaactgg cagctgattg ccggagaatt
      301 acagcatttt ggtggcgata gtatcgccaa caaactgcgc ggacacggta aattgtatcg
      361 ggccattttg ctcgatgttt caaagcgatt gaagctgaaa gccgacaaag agatgtctac
      421 gtttgaaatt gagcaacagt tactggaaca atttctgcgt aatacctgga agaaaatgga
      481 cgaggaacat aagcaggagt ttctgcacgc ggtcgatgcc agggtgaatg agctggaaga
      541 gctgctgccg ctgctgatga aagacaaatt attggcaaaa ggtgcgtcgc atttgctttc
      601 cagccaactg acccgcattt tacgcaccca cgcagcaatg agcgtacttg ggcatggttt
      661 gctgcgcgga gcggggctgg gaggccctgt gggcgcagca ttaaatgggg ttaaagcggt
      721 tagcggcagc gcctatcgcg tgacgattcc agccgtactg caaatcgcct gcctgcgccg
      781 gatggttagc gccactcagg tctgataccg gaaaccgggg aaaattttca ttttcccctc
      841 aaaagatcgt agacactgcc ccactggctg attattatgc cgcgccctga aaacactaca
      901 gttattcagg gaaattattt caccattcat tcgatgatga tttttgagga attatgggca
      961 acactaagtt ggctaatccg gcaccgctgg gcctgatggg cttcggcatg accaccattc
     1021 tgcttaacct gcacaacgtg ggttatttcg ctctggacgg tattattctt gccatgggca
     1081 ttttctacgg cggcatcgcg caaatttttg ctggtctgct ggagtacaaa aaaggcaaca
     1141 ctttcggttt aaccgcattc acctcttacg gttctttctg gctgacgctg gttgcgattc
     1201 tgctgatgcc gaaactgggt ctgaccgatg cgccaaatgc acagttcctt ggtgtctacc
     1261 tgggtctgtg gggcgtattt acgctgttta tgttcttcgg cactctgaaa ggcgcacgcg
     1321 tcctgcaatt cgttttcttt agcctgaccg tgctgtttgc cctgctggcg atcggtaaca
     1381 ttgccggtaa tgccgcgatc atccactttg ccggctggat tggtctgatc tgcggtgcca
     1441 gcgcaatcta tctggcgatg ggtgaagtgc tgaacgagca gtttggtcgc accgttctgc
     1501 cgattggtga atcccactaa ttgtcttgag atccccccgc tcggggggat ttttttattc
     1561 gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca accacttctg gtgccgtttc
     1621 aacgtatggc ccttccagca actgaatgca gtacggtacg ctggcaaaaa taccatgcac
     1681 cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc tctttaatag acttcggctg
     1741 accgggtaag ttaaggatca gcgcctgttt acgaatcacg cccacctgac gcgaaaggat
     1801 cgcagttggt acaaaatgca ggctgatctg gcgcatctgt tcaccaaagc caggcatctc
     1861 gcggtccgct actgccagcg tcgcatcggg cgttacgtca cgacgcgccg ggccagttcc
     1921 gcccgtggtg agcaccagat ggcaactcat ttcatccacc agctcacaca acgtttgctc
     1981 gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt tcaaacgccg tggttagcgc
     2041 cgatgtcagc cattcttcca gcgctggaat gcctttatcc tgataaacgc cgctggatgc
     2101 gcgatcagag atggaaacta agccaatacg taaagtattc atcaagattt ttccgtttaa
     2161 acagtctcgt taaacagaat gatacactgc gaagggagtg acagacaggc ttcagaagag
     2221 gtagcgtgac cgacttcccg gtcacgctaa gaatgattac agcagatcgc cgaccatttt
     2281 ttccagtttt tcctggtcaa tagcaaactt acggatacct tccgccagtt tatctactgc
     2341 cattggatcc tggttgtgct gccacaggaa ctcggactca gtgatacgcg ccggacgcgc
     2401 tttcacttcg ccggtgtaag acagtttacg ttcgatagcc ccttcgctct ccgccagctc
     2461 tttcagcagt gccggtgcga tggtcagacg gtcacagcct gccagttcca gaatttcgcc
     2521 gatgttacgg aagcttgcgc ccataaccac ggtttcataa ccgtgctctt tgtagtactg
     2581 gtagatttca gatacagaaa ccacgcccgg atcttctgcc ggagcgtact ctttcttatc
     2641 ggtattcgct ttgtaccagt caagaatacg gccaacaaac ggcgagatca ggaacacgcc
     2701 cgcctccgca caagcacgag cctgagcgaa ggagaacagc agggtcaggt tacagttgat
     2761 accttctttt tccagctgtt ctgcagcacg gataccctgc caggtagaag ccagtttgat
     2821 cagaatacga tcgttgctaa tacctgcatc gttgtagagt ttgatcaggc gttttgcttt
     2881 cgcaattgac gcttcggtgt cataggaaag acgcgcatca acttcagttg agatacggcc
     2941 cggaaccagt ttcaggattt ccagaccaat atttactgcc agtttgtcgg tcgcgtccac
     3001 gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag gcgacagcat catcaatcaa
     3061 cttacggtat tccggaatct gcgctgcgtt aagaatgaga gaagggttgg ttgtggcatc
     3121 ctgcggttga tacagcttca ttgccgcgat gtccccagtg tcggccacta cggtggtgta
     3181 ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt ctctttaaac agcttgttag
     3241 ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa cgtcagcgca accgccgaca
     3301 agacggttat cgccaggcga gactgtttcg gatttctgac actgcgttga tatcgcccgc
     3361 catttttata caaaacctca tgtatgctac gcagaagtta tcaagtacct cgtagcgtat
     3421 atacttctta aacaattggt aacgtttaca caggaaagtc atcgcgaccg gcaataagag
     3481 ggatatgcat gccagatttt ttctccttta ttaacagcgt cctttgggga tcggtaatga
     3541 tttacctgct cttcggcgca ggttgttggt tcacttttcg caccggattt gtgcagtttc
     3601 gctacatccg ccagtttggc aaaagtctta aaaatagcat tcatccacag ccaggcggtt
     3661 taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg cgtgggtagc ggcaacctgg
     3721 ccggcgttgc gctggcaatt accgccggtg gaccaggtgc cgtcttttgg atgtgggttg
     3781 ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc ccttgcacaa ctttataaag
     3841 aacgtgacgt caatgggcag tttcgtggcg gaccggcatg gtatatggcg cgcgggctgg
     3901 ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct catcgcctac ggcataattt
     3961 tcagcggagt tcaggcgaac gccgttgccc gagccctgag tttttctttt gattttcctc
     4021 cgctggtgac gggcattatt ctcgctgtct ttgctctgct ggcaatcact cgcggtcttc
     4081 atggcgtcgc ccggctcatg caggggtttg tcccgttgat ggcgataatc tgggtactga
     4141 ccagcctggt gatttgcgta ataaatatcg ggcaacttcc ccacgtcatt tggtctattt
     4201 ttgagagtgc ttttggctgg caggaagcgg caggcggcgc ggcgggatat accttaagcc
     4261 aggcgattac taacggtttt cagcgcagta tgttttccaa tgaggcggga atgggatcga
     4321 cgccaaacgc ggcagcggca gcggcagcgg cagcggcagc ggcagcggca gcggcagcgg
     4381 cagcggcagc ggcagcggca gctgcatcct ggcctccgca tccggcagca caagggattg
     4441 tccagatgat tggcattttt atcgacaccc tggtcatctg tacggcaagc gccatgctga
     4501 tattactggc gggtaacggc acaacctaca tgccgctgga aggtattcag cttatccaga
     4561 aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt tgttaccctc gtggttattc
     4621 tgtttgcctt cagctccatc gttgccaact acatttatgc cgaaaacaat ctcttctttt
     4681 tacgcctgaa caaccctaaa gcgatctggt gtttgcggat ctgcaccttc gcaacggtca
     4741 tcggcggcac cttgctaagc cttccgctga tgtggcaact ggcagatatc ataatggcct
     4801 gcatggctat taccaatttg accgccattt tactgctctc gcctgtggtt cataccattg
     4861 ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc ggtgtttgat ccgttgcgtt
     4921 atccggatat cggtcgccag ctttctccgg acgcgtggga tgatgtttcg caggagtaat
     4981 cacaactatc gatcaactca ttctcttttt ttgctaaagt cggcataaat ttcctgcaag
     5041 gactggatat gctgattctt atttcacctg cgaaaacgct tgattaccaa agcccgttga
     5101 ccaccacgcg ctatacgctg ccggagctgt tagacaattc ccagcagttg atccatgaag
     5161 cgcggaaact gacgcctccg cagattagca cgctgatgcg catcagcgac aaactggcgg
     5221 gtatcaacgc cgctcgcttt catgactggc agccagattt cacgccggcg aatgcccgcc
     5281 aggcgattct ggcgtttaaa ggtgatgtct acaccggctt gcaggccgaa accttcagcg
     5341 aagacgattt cgattttgcc caacagcatt tgcgaatgct ttccggcttg tatggcgtac
     5401 tgcgcccgct cgatttaatg cagccttatc gtctggaaat ggggatccgt cttgagaatg
     5461 cccgagggaa agatctgtat caattctggg gagatatcat caccaacaag ctgaacgagg
     5521 cgctcgcagc acaaggcgat aatgtggtga ttaatctggc gtcagatgag tattttaaat
     5581 cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc ggtgttcctc gatgagaaga
     5641 acggcaaatt taagatcatc agcttctacg ctaagaaagc acgcggtctg atgagtcgtt
     5701 tcattattga aaatcggctg accaaaccag agcaactgac tggttttaat agcgaaggtt
     5761 acttctttga tgaagattcc tccagcaatg gcgaactggt gtttaaacgc tacgagcagc
     5821 gttaagtttg tagtcaatgc cggatgcggc gtgaacgcct tatccggcct acaaaagcat
     5881 gcagattcaa tatattgcag aattaacgta ggcctgataa gacgcgccag cgtcgcatca
     5941 ggcgctgaat gccggattgt tacccggcat ttgtcattta gcggtgatgt ttgcctggac
     6001 catgaccgcc gtgatgatca tgaggagctt tcttatggtg gcgcggcggt ggcggcggtc
     6061 cgtgtgggtg ccagcgattg cctcgccatt cataatgttg tttccaccag ccgtggtcgc
     6121 gccagtgacc tccatcccag taatagccac gattatcacg atcgcctatc tgtaatttta
     6181 ctgacgggac taacgtaatt tccgcagcct gtgctgccat gggagcgacc agaaccaggg
     6241 aaagtgcgag tacgatagat tgcatctttt tcacaggtta ctccttttat cattgcccgt
     6301 tatgggtcga tatccggaga ttacgcgatg agaacgacgg ttgttattct ccgcaatcct
     6361 aatctctaag ttaccgcatt tattgataat ttctcctttt tccctgtcgt ttttctccat
     6421 aatttcttca taaaaaagcc gggctgcata aaagcaaacc cggcctgatt gagataatga
     6481 atagatttta ctgatgattc atcatcaatt tacgcaacgc agaaaaatcg gcgggcagat
     6541 tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc ttttggcaga tccaacgttt
     6601 caccgagaat cgcttccacg ctctctttaa atttcgccgg atgcgcggtg ccgaggaaca
     6661 agccatattc gcctggattc aactggtcac gcagcgcacg atacgctacg gcagcgtgcg
     6721 gctccgaggt gtagcccagt tcttttaact cacgcattgt ctgttgcgtg gtttcatcat
     6781 ccacggctgc ataacccagc tctttcagtt gccagatttt gcggcggaac aactcttcca
     6841 cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt ggataacgtc gcctgagtcg
     6901 ctttgggtga ccactgaccg tcgtgcagga aacgtggcac ggtatcgttc acgttggtcg
     6961 cagcaataaa acgtttcacc ggcagaccga atgacttcgc cagcagaccc gccgtcaaat
     7021 cgccgaagtt tccgcttggc accgagacaa ccagctggtt gcgcgcctcc tgcggcagct
     7081 gcgcaacagc ttcaaagtag tagcaaatct gcgccagcaa acggctgatg ttaatcgagt
     7141 tagccgagtt taaccccagc gccactttca gttcttcatc atcaaacgcc tgcttcacca
     7201 gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt ttcgatattg ccgcccaacg
     7261 tacagaacag tttttcttgc agtggactga ttttgcctcg tggatagagg ataaccactt
     7321 tcacattcgg caaaccgtag aaagcatgag ccactgcagc tccggtatca ccggaggtcg
     7381 cggtcagaat ggtcactggc ttatcatccg caatatgggt cagcatttgt gccataaagc
     7441 gaccgccgaa atctttaaat gccagcgttg gcccgtggaa caattccaga caaccgacat
     7501 cgctttcaac attggcgacc ggagccggga aggcaaacgc cgcgcgcacg cgctcttcca
     7561 ggatttcctg tgggatttca tcaccaataa acgccgagag gatcttcgca ctgcgggtga
     7621 caaaatccag cttcagcatc tcatcaattt cagtcaggct gaattccggc aggtcgtgcg
     7681 gaaaaaacag cccctgattt ttgcccaacc cctgggttac ggcttgcgca aagctgacct
     7741 gctcgttgtg atctttcaga ttgtagagtt tcatttagtt ttccagtact cgtgcgcccg
     7801 ccgtatccag ccggcaaata tgaacaaaac cttcctgatt ttgcaggtag ttcttaccca
     7861 accagtcggc aacgcgctgg gcggtatccg gcttgtcaca tagagcgaac aaggtcgggc
     7921 cggagccgga gataccgctc gctaccgcgc cgatttccgc gaccgcctgc cgcgcctgcc
     7981 ggaagccagg cagtaaccgt tcacggtagg gttcagcgat aacatctttc atcagcttcg
     8041 cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa gccagccaga tgtcgcccgt
     8101 gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat agcccgggct tctgccgtcg
     8161 agactttaat tcccggatac gccagcaccc acagccactc atcaaaccct ggcacttgct
     8221 ggctgatgat gtcgttttct tcgatcatca actgcatacc accaagaaaa cacggtgcca
     8281 cgttgtcgta atgaatgctg ccggagatac gtccttccag ctcgcccatc aaagccagca
     8341 aacgagtgtc attaagcggc ttgccgcagt gttcattcat cgccatcagc gccgcgacca
     8401 ccgaacaggc gctggagcct aagcccgaac cgatcggcat attcttttcc agagtcatcg
     8461 ccactggaat ttgcttgcca agctcctggc aaaaacgctc ccagcactga taaacgatat
     8521 tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc gaggttgttg agactgaatg
     8581 tctctgccgc ctcaaccgtg actacatctc cgagcaatgc accatcaacg ggtgccaccg
     8641 ccgccccgag cacatcaaac ccgacgctca tattggcact ggaagccggg gcataaactt
     8701 taaccatgtc agactcctaa cttccatgag agggtacgta gcagatcggc aaagacaccg
     8761 gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca ccaacggcag cggctgataa
     8821 tagtggctat aaaaggccag ggcgttttcg ccatttttca ctttgaacag cggatcattc
     8881 ccatccactt cggcaatctt cacgcggcag acaccatctt catcaatatt gccaacatag
     8941 cgcaaaactt ttccttcatc acgggccttc gccacgcgcg cggcaaagag atcgtcgagc
     9001 tgtgacagat tcgccataaa agcggcaaca tcaccctcgg cgttaaactc tgcgggcagc
     9061 acaggttcaa tttcaatatc cgccagctcc agttcacgtc ccgtttcacg agcgagaatc
     9121 aatagtttac gcgccacatc cataccagaa agatcatctc gcgggtccgg ttcggtataa
     9181 cccatttccc gcgccagcgt ggtcgcctcg gagaaactca tgccttcgtc taacttgccg
     9241 aagatataag aaagcgaacc tgaaagaatg ccggagaact tcaccaattc atcacctgca
     9301 ttgagcagat tttgcaggtt ctcaataacc ggtaatccag ccccaacgtt ggtgtcatag
     9361 aggaatttac gccgcgattt ttccgccgca taacgcaact gatgatagta atccatcgac
     9421 gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac cttcgcgcag gaagtcggca
     9481 tattgatccg ccactgcctg gctggaggtg cagtcaacaa tgaccgggtt cagcagatga
     9541 tattctttca cgaggcgaat taagcgcccg agattaaacg gctctttggc ttgtgcaagt
     9601 tcttcctgcc agttttccag attaaggcca tgcacactag tgagcagagc cttcgagttg
     9661 gcaacgccgc agacacgtaa gtcgatatgt ttattcttca gccagctttg ctgacgcttc
     9721 agttgctcca gcagcgcacc gccaacgcca ccgacgccaa tcacaaacac ttcgataacc
     9781 tgatcggtat tgaacagcat ctgatgagta acgcgcacgc cagtggtcgc atcatcgtta
     9841 tttaccacga cagagattga gcgttcagaa gatccctgag caatggcgac aatgttgata
     9901 ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc cacgcaaggt gcgcatacca
     9961 tcacctacca ccgagataat ggccagccgt tccgtcaccg ccagcggctc cagtaagcct
    10021 tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt cagctcgcac acagtcgctt
    10081 tgtggaacgc agaaactgat actgtattcg gaagatgatt gcgtaatcag caccacggaa
    10141 atacgggcgc gtgatattgc agcaaagacg cgcgccgcca tgccaaccat ccctttcatc
    10201 cccgggccgg aaacgctgaa cattgccatg ttattcagat tggaaatgcc cttgaccggt
    10261 aattcgtctt catcacggct ggcaccaatg agcgtacctg gtgcttgagg atttccggta
    10321 tttttaatca ggcaagggat ctggaactgg gcgatggggg taatggtgcg ggggtgaaga
    10381 actttagcgc cgaagtagga aagctccatc gcttcctggt aggacatcga cttcaacaac
    10441 ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc cgtcaacgtc cgtccaaatc
    10501 tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg cggagtagtc ggaaccgtta
    10561 cgtccaagca ccaccagttc gcctttttca ttaccggcgg tgaaacctgc catcaacacc
    10621 atgtgatcag ccggaatgcg gcttgccgca atacggcggg tggactcagc aatatcgacg
    10681 gtagattcga ggtaatgccc cactgccagc agtttttcga ccggatcgat aacggtaacg
    10741 ttgtgaccac gcgcttctaa cacgccggcc ataatggcga tcgacatttt ctcgccacgg
    10801 caaatcagcg cagcgttgat gctatccggg cactgcccca acaaactaat gccatgcagg
    10861 acatgtttta tttgggcaaa ttcctgatcg acgaaagttt tcaattgcgc cagcgggaac
    10921 cccggctggg cggcggcgag tcccgtcaaa agttcggcaa aaatacgttc ggcatcgctg
    10981 atattgggta aagcatcctg gccgctaatg gttttttcaa tcatcgccac caggtggttg
    11041 gtgattttgg cgggggcaga gaggacggtg gccacctgcc cctgcctggc attgctttcc
    11101 agaatatcgg caacacgcag aaaacgttct gcatttgcca ctgatgtacc gccgaacttc
    11161 aacactcgca tggttgttac ctcgttacct ttggtcgaaa aaaaagcccg cactgtcagg
    11221 tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc accgttacct gtggtaatgg
    11281 tgatggtggt ggtaatggtg gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt
    11341 ttatctgtct gtgcgctatg cctatattgg ttaaagtatt tagtgaccta agtcaataaa
    11401 attttaattt actcacggca ggtaaccagt tcagaagctg ctatcagaca ctcttttttt
    11461 aatccacaca gagacatatt gcccgttgca gtcagaatga aaagctgaaa aatacttact
    11521 aaggcgtttt ttatttggtg atattttttt caatatcatg cagcaaacgg tgcaacattg
    11581 ccgtgtctcg ttgctctaaa agccccaggc gttgctgtaa ccagtcgacc agttttatgt
    11641 catctgccac tgccagagtc gtcagcaatg ccatgactcg ttcgcgtaaa gcttgcagtt
    11701 gatgttggtc tgccgttgtg tcacttttcg ccggttgttg tattaatgtt gctaattgat
    11761 agcaatagac catcaccgcc tgccccagat tgagcgaagg ataatccgcc accatcggca
    11821 caccagtaag cacgtcagcc aacgctaact cttcgttagt cagcccggaa tcttcgcgac
    11881 caaacaccag cgcggcatgg ctcatccatg aagatttttc ctctaacagc ggcaccagtt
    11941 ctactggcgt ggcgtagtaa tgatatttcg cccgactacg cgcagtggtg gcgacagtga
    12001 aatcgacatc gtgtaacgat tcagccagtg tcgggaaaac tttaatatta tcaataatat
    12061 cactagatcc atgtgcgacc cagcgggtgg ctggctccag gtgtgcctga ctatcgacaa
    12121 tccgcagttc gctaaaaccc atcgttttca ttgcccgcgc cgctgcccca atattttctg
    12181 ctctggcggg tgcgaccagg ataatcgtta tacgcatatt gccactcttc ttgaccaaat
    12241 aaccgcgaac agggtgatca ctgtcaactt attacgcggt gcgaatttac aaattcttaa
    12301 cgtaagccgc agaaaaagcc ctttacttag cttaaaaaag gctaaactat tttctgactg
    12361 cactaacggt ttaattgtta aaaaatgcta catatccttc tgtttactta ggataatttt
    12421 ataaaaaata aatctcgaca attggattca ccacgtttat tagttgtatg atgcaactag
    12481 ttggattatt aaaataatgt gacgaaagct agcatttaga tacgatgatt tcatcaaact
    12541 gttaacgtgc tacaattgaa cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc
    12601 ggcccctctt agcctgttat gttgctgtta aaatggttag gatgacagcc gtttttgaca
    12661 ctgtcgggtc ctgagggaaa gtacccacga ccaagctaat gatgttgttg acgttgatgg
    12721 aaagtgcatc aagaacgcaa ttacgtactt tagtcatgtt acgccgatca tgttaatttg
    12781 cagcatgcat caggcaggtc agggactttt gtacttcctg tttcgattta gttggcaatt
    12841 taggtagcaa acatgcagac cccgcacatt cttatcgttg aagacgagtt ggtaacacgc
    12901 aacacgttga aaagtatttt cgaagcggaa ggctatgatg ttttcgaagc gacagatggc
    12961 gcggaaatgc atcagatcct ctctgaatat gacatcaacc tggtgatcat ggatatcaat
    13021 ctgccgggta agaacggtct tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg
    13081 ttgatgttcc tgactggccg tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt
    13141 gcagatgact acatcaccaa accgttcaac ccgcgtgaac tgacgattcg tgcacgcaac
    13201 ctgctgtccc gtaccatgaa tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc
    13261 tacaagttca atggttggga actggacatc aacagccgtt cgttgatcgg ccctgatggc
    13321 gagcagtaca agctgccgcg cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca
    13381 ggcaaaattc agtctcgtgc tgaactgctg aagaaaatga ccggccgtga gctgaaaccg
    13441 cacgaccgta ctgtagacgt gacgatccgc cgtattcgta aacatttcga atctacgccg
    13501 gatacgccgg aaatcatcgc caccattcac ggtgaaggtt atcgcttctg cggtgatctg
    13561 gaagattaat cggctttacc accgtcaaaa aaacggcgct ttttagcgcc gtttttattt
    13621 ttcaacctta tttccagata cgtaattcat cgtccgttgt aacttcttta ctggctttca
    13681 ttttcggcag tgaaaacgca taccagtcga tattacgagt cacaaacatc atgccggcca
    13741 gcgccaccac cagcacactg gttcccaaca acagcgcgct atcggcagag ttgagcagtc
    13801 cccacatcac tccatccagc aacaacagcg cgagggtaaa caacatgcta ttgcgccagc
    13861 ctttcaatac cgcctgcaaa taaataccgt tcattaacgc cccaatcaga ctggcgatta
    13921 tccatgccac ggtaaaaccg atatgttcag aaagcgccag caagagcaaa taaaacatca
    13981 ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa acgttgcgcg gtgagcgttt
    14041 caaaaacaaa gaacgccata aaagtcagtg caatcagcag aatggcgtac ttagtcgccc
    14101 ggtccgttaa ttggtattga tcggctggcg tcgtcactgc gacgctaaac gcggggaagt
    14161 tttcccagcc ggtatcattg cctgaagcaa aacgctcacc gagattatta gcaaaccagc
    14221 tgctttgcca ttgcgcctga aaacctgact cgctagcttc ccgtttggct ggtagaaaat
    14281 cacctaaaaa actgggatgc ggccagttgc tggttaaggt catttcgcta ttacgcccgg
    14341 caggcaccac agaaagatcg ccggtaccgc ttaaattcag tgccatattc agctgcaggt
    14401 tctgcttccg ccagtcccct tcgggtaaag ggatatgcac gccctgcccg ccttgctcta
    14461 acccggtacc gggttcaatg gtcagtgccg ttccgttaac ttcaggcgct ttcaccacac
    14521 caataccacg cgcatccccg acgctaatca caataaaggg cttgcctaag atgatatttg
    14581 gcgcgttgag ctcgctaaga cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt
    14641 gccagacctg accggtataa atccctatct tgcgttcttc cacgttctga ttgccatcaa
    14701 ccatcaatga ctcaggtaac caaaaatgga taaaacttcg tttccgctcc acggttttat
    14761 cctcttcctg caccgtataa agctcggtca caggaatagc gataagcggc ccaacgagtt
    14821 tttgcggccc gctggtactt tggcgaatcg catcttccac atcgctacgg taatcggcac
    14881 gttcgacaat cacttgccga atcagcatta tcggaatcaa caacagcaat actgcaccaa
    14941 acaaggtggt cattttccag aacagggggg atttcaacat agtcttctcc tttgcaatgg
    15001 cagcaggata cgaaggctat gtgggaagaa tttgaagcta tgtgaagtta cggtgaagtc
    15061 gtagcgaggc cagcacgcca ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca
    15121 aacgggcgac ctcactgacg aacgccaacc ccagaccgct gcttttttgc ccatttgcac
    15181 gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc gtagtcagga ataccactac
    15241 cggtatccag caccttaagc gtgacgtgtt cctgatccac ttcggcgctt agcgttatgc
    15301 aaccgctctc gggagtaaaa tcgatggtgt tatccagtaa attccccagc gcctgatcca
    15361 gtaacgccgg ttcagcagca acattaacct cggtgggcat aacatgcaaa gtgatgtttt
    15421 tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa
    15481 cagcagtcag aacgacttcc tgacgattct ccagtcttgc ctggcgtagt aacgtttcca
    15541 ccagcgcctg cattcgcgca ttttgtgtca gaatgttgtc agtaaaacga gccactactt
    15601 caggcggcgg accttcgcgt aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt
    15661 ttagctcatg ggttaacgca taaacatact gctcaatata gtttttccct tccagcttca
    15721 cgcgcatact ttccagcgcc tgcgcgagtt tacgcagctc gctactaccg agatcgggaa
    15781 gaggaacggg cttattgtca gtgacggaat cagcatagcg agtgagccgg gcaatggagc
    15841 ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc aatccccaac aaaatggcgc
    15901 tggcccataa aattcgccgc tcgctgcgtt taatgaccgg agccatcgcc gcgttcggtt
    15961 tgcctacgct caaaacgcca ataagccgcg agccgcccat aatcggcgcg gcaacataca
    16021 tcaccgaact ttcgggatcg gcaggatttt gcaacgtgct gcgcgcacca tactgaccac
    16081 gcaacgttag ccagacgtca ttccagcgcg aataatcctg tccaacggct ttatttgccg
    16141 aatcgaacaa tactttgcca tgcgcatcgg tcatatagac atgatattca ttgcgcactt
    16201 tgttaatgcc accaatattg gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg
    16261 ccagttgccc atgcgttggg tccccagaga gcaaatcggg acgcgccagc tccgcaagta
    16321 acgttgccgt gtcgatcaac gtcccctccg ttgctcttcg cacgcccggt ttaacttctt
    16381 tgacaaaaat agccagtacg aaccaggccg ccaccgccac cagtaaaaaa tagcccagca
    16441 gcaaccgcat gccgatacgc attacaagcc cctcaggcta tatcccatgc cgcgatgagt
    16501 attaatcggt gaaagatcgg ggttgatggc gcgcagcttg gcacgcagcg ttttaatgtg
    16561 gatatcgacg gtgcgatcgt aggtgtcctg cgcatcttcc cacacgctat ccattagttg
    16621 ctggcgggac cagacgcggc ctggcgactt gagtaacgtc ttcagcaata aaaactcata
    16681 ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc gccgcgggtt cattcaattc
    16741 aaaatgacca atacggatga cgggagacgg cgtcgagaac ttcttcaccc gacgcagtaa
    16801 ggtgcgcacc ctggcgcaca cttcgcgggg tgaaaacggt ttagccacat agtcgtcaac
    16861 gccaatttcc agcccaagca ggcgatcgac ctcttcactt cgggccgtca ggaacagtac
    16921 aggtaacgcc ggatggagcg ccagtaactg gcggcacaat tcaaagccac tgatatcagg
    16981 caaaccaacg tcgagaatca tgacgtcggg aacctgctgg cgagctttat ccagcaccgg
    17041 caaacctcgc tcaaagacct cgacggcaaa accttcctgc tgcaacatgt agaccagcgt
    17101 gtcggctatc ccttgctcat cttccactaa ccagaccgtt tcccgttgca taaatcgcct
    17161 ctgttattgc cgccacggca tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc
    17221 tacaactttg tcggagtaag ccagataagc gagcgcgttg cgtttggcgt cataaaagcg
    17281 cacgacctgt aacgacttaa agaccaggga cgtgcgtttt ttgaatacta cctcgccctg
    17341 agctttgccg tttttaatac gatccgacag ttcaatcggc ccgacttgct gacaagaaat
    17401 ggccgcatcg gaggtatctt ctgccaggcc caatccccct ttaataccac cggttttcgc
    17461 ccggctcaca taacaggtga catttttcac atcgggatcg tcaaaggctt ccacaacaat
    17521 tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg
    17581 agccaatggc cccagcatta tcaggcttaa agacaggatc aaatgcttgt atttcatatt
    17641 gttaccattt ttatctatta cacgagcaat tagttcgtca cggtaaataa catattgatt
    17701 aagaacaaaa tctcaattct tttatttccg tcaggcccta aaacatactc tactaagaaa
    17761 aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca attacctgat gtcaggtgct
    17821 cgttgttgaa aggatgagga tattttatgg atcaggccgg cattattcgc gaccttttaa
    17881 tctggctgga aggtcatctg gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag
    17941 gttattccaa gtggcactta cagagaatgt ttaaagatgt cactggccat gctattggcg
    18001 cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc
    18061 cgattctgga catcgcgctg caataccgct tcgactctca acagacattt acccgcgcat
    18121 tcaagaagca gtttgcccag actcctgcac tttaccgccg ttctcctgaa tggagcgcct
    18181 ttggtattcg cccgccgcta cgtctgggta aattcacaat gccagagcac aaatttgtca
    18241 ccctggaaga tacgccgctg attggtgtta cccagagcta ctcctgttcg ctggagcaaa
    18301 tctctgattt ccgccatgaa atgcgttatc agttctggca cgattttctc ggcaacgcgc
    18361 cgaccattcc gccggtgctc tacggcctga atgaaacgcg tccgagtcag gataaagacg
    18421 acgaacaaga ggtattctat accaccgcgt tagcccagga tcaggcagat ggctatgtac
    18481 tgacggggca tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc
    18541 tgggaaccgg cgtgcaggag tttatcctga cggtatacgg aacgtgcatg ccaatgctca
    18601 atctgacgcg ccgtaaaggt caggatattg agcgatacta cccggcagaa gacgccaaag
    18661 cgggagatcg cccaattaat ctgcgctgtg aactgctgat tcctatccgc cgttaacgct
    18721 gcagctcatc taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc
    18781 agccagacgc cagccacagg ctttcctgat aatccacgcg cgaaatagaa cagttacgca
    18841 gacgtaagcg gcgctctgcc catgctggta atccgagaat cgtactcacc agacatccca
    18901 gtgcaatacc atggctcacc agcagcggtc ggcttccctg cggtaagtcc cggcaagatt
    18961 ccagtgccgc attgacgcga tcgctgagct cctgcattga ctcgccttca ggaatacgcc
    19021 cgtcaacggt gccattgacc agctgccgac gccagttctc ttcttcttcg gtcagagaat
    19081 cgatatgtct tttttccagc acacccatgt ttaattcacg caggcgagaa tcaaagatga
    19141 tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc
    19201 tactgataat atgcgtaatg ccaagctctt tggcacgggt tgccacctgc atcgcctgtt
    19261 gctcaccttt ggcggtcagc gggctgtcag actggccctg aatacgtcgc tcggcgttcc
    19321 actgcgtttc accgtggcgg actaggtata cctgtaacat gctttttttc cgtatactgc
    19381 tgtaacgtta attttctgag taatgctgat tatgcaccaa gttgtctgtg cgaccaccaa
    19441 tcccgctaaa attcaggcca ttctgcaggc atttcacgag atcttcggcg aaggatcctg
    19501 ccatattgca tccgttgccg tcgagagcgg tgtaccggaa caaccctttg gcagtgagga
    19561 aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc cgtttacttc cagaggctga
    19621 tttttgggtg gcgattgaag ctggcattga tggcgacagc actttcagtt gggtggtgat
    19681 tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact ttgccgttac ctgcggtgat
    19741 tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg atgtcgcgtt ataccggtat
    19801 tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt accgccggga aactcactcg
    19861 cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt ccgtttcaca atgccgtgta
    19921 tcaggcattg taggacggat aaggcgttca cgccgcatcc gacattcggt gcacgatgcc
    19981 tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaatca atcgcttttc agcaacacct
    20041 cttccagcca ctgacgcagc tccacgggcg cggctttcag gctgttagat ccacgcgtaa
    20101 tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg ctgactcatt tcgccacgca
    20161 acagctcttc gacaatacgc acgcgagtcc ccaacgcttc gcgctcatct ggcgtcagca
    20221 tcaggtttaa caacggtaaa tggagatcgt tttggtaggc attcttaagc aggtcgacaa
    20281 aacgtaacca ctcctggtga cgctgttctg ccatcgctgc tgaatagggt gattgttggg
    20341 ccataatatg tcgccattgt tagcggggga agcaaaatga ctcccccggt tgtactcgct
    20401 aaagagtacg atagcatatc ataaacgtgc ggatcagtaa cgacgtcccc attccgtggc
    20461 gctcatcaac gtcggtttat cccccatgaa atagcggtag taagcatcat aagccagcac
    20521 gttcttcaca taaccgcgcg tctcggagaa cggaatactc tcgacaaatg ccacagcgtc
    20581 gatacgcccg gcgctgttgc caagccaggt tcgcacccgt ccaggtccgg cgttataagc
    20641 tgctgaggag aaaatacggt tattgccaaa ctgctgataa acatattgca ggtaactggt
    20701 gccaatgttg atgttcgttt ccggatccag caattgccca gggctgctat aaccaggaat
    20761 agagaacatc ttcaccgtat gggtcgctgt accaggcata atctgcatca ggccgctggc
    20821 ccctaccggt gatttcactt tcggattcca ggcgctctcc tgacgggcaa tcgccatcgc
    20881 atagctttgc gggatctctt tcccactggt gtaacgtttg aaaagatcgt tgtaagccag
    20941 cgggaatcgc tcttccagat gatcccaaag cttcccggcg atcgttgcct gaacgctaag
    21001 atcccaccat tggttattga aagcataccg agccagttga gcctgctctg tttttgactt
    21061 gctcttcacc agattggccc actcgctacg cgcggtatta tcgagattcc agtacatcaa
    21121 ctcgcgcacg cgcgccatct ccggcccctg agtcagggtg ctgtcaacat tctgcggcgc
    21181 tttatcaatc ttcagctcat actcttcacc gatgcgttgt gctgcaacca tcgggtagaa
    21241 accacgctgt tgcatgagtt gatgcaaaat ctctttcgct tcagcttcac gtccgcgttc
    21301 cagcaataaa tccgcctgcc agtaacgcca ttcatctttc tcttttgctt ccatcggcag
    21361 acgcgccagc caggtattca ggccgcgacg atcgccggtg ccaagcgcca ttcgcacacg
    21421 gcgttcaatc aacgacgtgg attgcgagcg cataatggcg tcatcgcgcc atttcgcctg
    21481 ctcatcggtg acatcattgc ccatcaaacg ccaggcgacg atatcgcgca gctcctgaat
    21541 ctgatcttca ttaagctgct gcgcctgggc aagcgacggg atcatcagcc gtgcattttc
    21601 ggcatcctgc cgcgccacac tggcaaacgc caccgccgcc atttgacggg taaaatcggt
    21661 cgcgccggtt gtacgcgcga aggtcagtac cgtattaggg ttgttcgcca gtgaaatgat
    21721 tgccgaggcg atagtctggt aatcggcagg catctgccct gccagcactg ttaccaggcc
    21781 tgtgttaccc gctttcatcg ccagacggat acgctctaaa tacgccagcg gatcttgttt
    21841 acctgacgca cgccagacgc taaataactt gtcacaggcg ttaggctggc tcttgccggt
    21901 tagccacagc tctttcgccc cttgccaggc ttcttcactc tgcccggtgt tccatttcgc
    21961 atagtagtaa ttacattgcg cttcggtagt tccgggcttt tccgggctaa aggctaacaa
    22021 gccacgccag tcttcacgcc gcgccagttc attgacgaaa cgagattgca gcgtgcgagc
    22081 gggaggaagc gtggggttag cgcgaacaaa gttagtgacc gtcaccgccg gttgattcat
    22141 caggtcatcg gtgatctggc ggtattccag gtagggataa agcggataat ccttcagtcc
    22201 aggcatcatt tgttcgacca catccatctg tcgattatcc caggcctgct tgatttgcgc
    22261 gtaacggcta cgctgctcat ccagtgagtc ggctcgcgcc acgctgctga ccgtcagcag
    22321 acagacaccg gcagccaaca gccgccaggt aacttgtttg gctttttcca caagcgcatc
    22381 ctctaagtgt aaatacatca atgcagcgtc ataccgcgta atagatttat gctaaccagt
    22441 cattgccgtt tacgccacgt tacggacact tttttacttt tactgcgaag gtgatcgacc
    22501 aactgggatt aggcggggaa acaggctaca cttcgccttt gaaaactcat catcacgata
    22561 aacatagagg cgaagtccaa cgtggctcaa ttcgtttata ccatgcatcg tgtcggcaaa
    22621 gttgttccgc cgaaacgtca tattttgaaa aacatctctc tgagtttctt ccctggggca
    22681 aaaattggtg tcctgggtct gaacggcgcg ggtaagtcca ccctgctgcg cattatggcg
    22741 ggcattgata aagacatcga aggtgaagcg cgtccgcagc cagacatcaa gattggttat
    22801 ctgccgcagg aaccgcagct gaacccggaa cacaccgtgc gtgagtccat tgaagaagcg
    22861 gtttcagaag tggttaacgc cctgaaacgc ctggatgaag tgtatgcgct gtacgccgat
    22921 ccggatgccg attttgacaa gctggccgct gaacaaggcc gtctggaaga gatcattcag
    22981 gctcacgacg gtcataacct gaacgtacag ctggagcgtg cggcggatgc gctgcgtctg
    23041 ccagattggg acgcgaaaat cgctaacctc tccggtggtg agcgtcgtcg cgtggcgttg
    23101 tgccgcctgc tgctggaaaa accagacatg ctgctgctcg acgaaccgac caaccacctg
    23161 gatgccgaat ccgtggcgtg gctggaacgc ttcctgcacg acttcgaagg caccgtggtg
    23221 gcgattactc acgaccgtta cttcctcgat aacgttgcag gctggatcct cgaactggac
    23281 cgcggtgaag gtattccgtg ggaaggtaac tactcctcct ggctggagca gaaagatcag
    23341 cgtctggcgc aggaagcttc acaagaagcg gcgcgtcgta agtcgatcga gaaagagctg
    23401 gagtgggtac gtcagggaac taaaggcagc cagtcgaaag gtaaagcacg tctggcacgc
    23461 tttgaagagc tgaacagcac cgaataccag aaacgtaacg aaaccaacga actgtttatt
    23521 ccacctggac cgcgtctggg cgataaagtg ctggaagtca gcaacctgcg taaatcctac
    23581 ggcgatcgcc tgctgattga tgacctgagc ttctcgatcc cgaaaggggc gatcgtcggg
    23641 atcatcggtc cgaacggcgc gggtaaatcg accctgttcc gtatgatctc cggtcaggaa
    23701 cagccggaca gcggcaccat cactttgggt gaaacggtga aactggcatc ggttgatcag
    23761 ttccgtgact caatggataa cagcaaaacc gtttgggaag aagtttccgg cgggctggat
    23821 attatgaaga tcggcaacac cgagatgcca agccgcgcct acgttggccg ctttaacttt
    23881 aaaggggttg atcagggtaa acgtgttggc gaactttccg gtggtgagcg cggtcgtctg
    23941 catctggcga agctgctgca ggttggcggc aacatgctgc tgctcgacga accaaccaac
    24001 gacctggata tcgaaaccct gcgcgcgctg gaaaacgccc tgctggagtt cccgggctgt
    24061 gcgatggtta tctcgcacga ccgttggttc ctcgaccgta tcgccacgca catcctggac
    24121 taccaggatg aaggtaaagt tgagttcttc gaaggtaact ttactgagta cgaagagtac
    24181 aaaaaacgca cgctgggcgc agacgcgctg gagccgaagc gtatcaagta caagcgtatt
    24241 gcgaagtaat gcgtaaaatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaacca
    24301 gcaatttcaa tacgttgtaa aactgtaggc ctgataagac gcggtaagcg tcgcatcagg
    24361 cattgagcac cgaatgccgg atgcggcgtg aacacctttt ccggtctaca acagccttag
    24421 catcctcaat ttcgtggcgg gtcacccgtg caggtttttt caaaccttgt ttaatctcga
    24481 cagcctcttc cagagaggtt ttcaactcat caaaaaaact cattttatac ctcgcctttg
    24541 agcagtttcg tcacggttat ccttgctccc ccatcatctc ccgcaccagc tccacgcagc
    24601 gcaggaaacg gctgtcgtaa tcgtcctctt caacccgcac gaattcgata ttgttctctt
    24661 ccagcatctc caccagcaag ttctggaact ctttgcgatc caccgaactg ccgaggctgc
    24721 gtaaaccatc cgccacccac ggcgtgttgt tctccagcag gatcaccaga tcgaaacggt
    24781 attcatcaat cagcgcctgc acgaacggat gctcacgccc ttcgtacttt ttgcagaacg
    24841 cctgggtagt gacaaaatcg gtgtcaataa acgccacttt attggcgtat ttcactgcaa
    24901 aatcaatgta ttgtgcgtgg cccaacgcga ttttatcgta gtcggaatac tgcaatgcga
    24961 tctcatcacc gccgaggtgt gaaaagacat aatcgcggcc atattcccac gcactggtgg
    25021 tgttgaagat attggcaagt ttgtttacca gggtggattt accgctcgac tcgccgccaa
    25081 ggatcgccac ggtacgcaca aaaaacggct tcacttcggt aggaatatat tcccagtagc
    25141 ggaacgggtt ttcgcggatc tgcgcaccgc tgatactcat aaaggtacgt ttcggatcaa
    25201 ccagcaccgt ctcgatcccc agatgttcca tatactgtgg cgcatcggct tcttccgagg
    25261 tgtagatcag gtccggctgg atcccctttt cagccataaa ctttttgatg ccgttgctcc
    25321 acacatccca gccatgcgga tacggctcca tgccctcttc gttgaaagca tgaatacgga
    25381 tatttttctg atatttaaag gtttgcaata gccagcgcag acgatccggc accgtgggct
    25441 gctgcgacat ggcgctgtct tcaaacaacg cgcggtcgcg ggtatcgtca aaacccataa
    25501 tgatatgcag ctcgtcaacc tggctacagg cgcgctggat aaggtagata tgtccggtat
    25561 gcagcgggta gaacttaccg aatacgacac caatggtttt cttctgccgg ggaaattcaa
    25621 gccccaaaaa acggtgcaat gcctccagct tttgcgcgct ggggctttta attttggcat
    25681 tcagtaactg gcttaaatac cctttggtca taccgctggc atcagctacc tgctgtagcg
    25741 tgcagccctg ttgcttgatg gcagttttca ggtaatcaaa tgacgacata tctccctccg
    25801 tatctctcat tataagtcgt cgaacacgct aagcgcgtcg gagagttttt taacgccaaa
    25861 aatctgcatc ccttccggcg cttttttcgg tacgttagcc gccggaacaa tcgcccggcg
    25921 aaaaccgtgt ttcgccgctt cagaaatccg ctcctggcca ctgggcaccg ggcggatctc
    25981 ccctgccagc ccgacttcac caaacaccac cagatcctgt ggcagcggtc tgtcacgcag
    26041 gctggaaacc atcgccagca gtaacgctaa gtcggcgctg gtttcggtta ccttcacgcc
    26101 gccgaccacg ttcacaaaca catcctgatc ggccatttgc agaccaccgt gacggtgcaa
    26161 gacagccagc aggattgcca gacggttttg ttccagcccc actgccacgc ggcgcggatt
    26221 cgccattatc gagtgatcga ccagcgcctg aatctccacc agcaacggac gcgttccttc
    26281 ccacaccacc atcacggagc tgccggaggt cacttcatct ccgcgactta agaaaattgc
    26341 cgaagggttg ctgacttcac gcagcccctg ttcggtcatc gcgaagacgc ccagctcatt
    26401 caccgcgccg aagcggtttt tatggctgcg taaggtgcga aaacgggagt cggcatcgcc
    26461 atccagaagc accgaacagt cgatacagtg ttccagcact ttcgggccag ccagcgaacc
    26521 atctttggtt acgtgtccaa ccatgacaat cgccacaccg cgcgttttgg cgaaacgcgt
    26581 cagataagcc gccgtttcac gcacctgcgc cacgctacca ggcgatgatt gtacatccgc
    26641 catatgcatc acctggatcg agtcaattac catcagcttc ggttgctctt cttcggcaat
    26701 caggcagatc tgctcgatgc tggtttccga caacatattg agattgtcag tcggcaggcc
    26761 aaggcgatga gcgcgcattg ccacctgttg cagcgactct tcgccggtga catacagcgt
    26821 tttcatctgc tgggccagtt tgcacagcgt ttgcagcaac agcgtggatt tccccgcacc
    26881 agggttaccg ccaatcagaa tggcacttcc tggcaccacg ccgccgccta gtacgcggtc
    26941 gaactcttta aatccggtgg aaaaacgcgg cagctcttca aggctgatat cggagagttt
    27001 ctggactttt gccaccccgg cgctaccggc atagccgctg agacgctcgt tacgcgccac
    27061 cgttggcgac gcagcaagac gcacctcggt gatggtgttc caggcatgac aggcactgca
    27121 ctgcccctgc cagcgcggat aatcggcccc gcattcatta caaacaaagg cgcgttttgg
    27181 agcttttgcc acggtgtacc tcgttaatgc tgtgccgccc gcaggatgac gggcaggcaa
    27241 ttacttctga ttcaggctgc cagagaggat gcagaatacc cccatcaggt cagcgtgacg
    27301 gatggtgact tccgcctttt cattcacttt tggcttggca tggtaggcaa tccccagccc
    27361 tgctgctttg atcatcggca ggtcattagc tccatcgcca atcgccacgg tctgcgccag
    27421 cgggatttca tactcctgcg cgaggcgagt cagagttttc gctttgtact gtgcgtctac
    27481 gatgtcgccg atcacattgc cggtaaattt accgtccatg atctccagtt cattggctac
    27541 cacggctgtc aggcgcagct tgtcgcgcag gtattcagca aagaaagtaa agccgccgga
    27601 ggcaatcgcc actttccagc ccagcgtttc cagcttgagc accagctgcg ttaagcctgg
    27661 catcagcggc agattttcac gcacctgttg cagaatattg gcgtcagcgc ctttcagcgt
    27721 cgccacgcgg ctgcgcaggc tggcggtaaa atcgagttcg ccgcgcatcg cccgttcggt
    27781 tacctccgct accatctcgc ccgttccggc cagcttggca atttcatcaa tacattcaat
    27841 ctggatggcg gtggaatcca tgtccatcac cagcaaaccc ggcgtgcgca ggtgcgggat
    27901 tttccccagc ggggcgacat ccagctgcgc ttcgtgggcc aggcgtgtag cccgtgcggt
    27961 gagtgaacct gccagacgaa tcacctgata atcttccacg caccaggcgg caacaatcac
    28021 catcgccgca cccagtttgc tctggtattg ggtcagacgc tgtttatcca gcccacgacc
    28081 atacagcagc cagccgctac gagctgcgtg gtaatccagt ggcatcactt catcaccact
    28141 taatgaaaga ggcagacccg gccataaaga gacatcttca ggcaggtcgc accaggtaat
    28201 gttaggcatt aaggctcctg taaaatcgtt cgaagcaggg aaaataacgc atgaggctac
    28261 cttgtatcca ttgcttctgg caacattaag tctcaaattt tcaaagggtg gaagatggct
    28321 cgcacaaaac tgaaattccg gctacatcgg gcagtgattg tcctgttctg tcttgccttg
    28381 ttagtggcac tgatgcaggg agcgtcatgg tttagtcaaa accaccagcg acagcgtaat
    28441 ccacagctgg aagaactggc ccgcaccctg gcgcgtcagg tgacgctgaa cgttgcaccg
    28501 ctgatgcgta ccgactcacc ggatgaaaaa cgcattcagg cgatcctcga tcagttaacg
    28561 gatgaaagcc gtatcctcga cgcgggtgtg tatgacgaac aaggcgatct tatcgcacgt
    28621 tctggcgaaa gcgtcgaagt gcgcgatcgg ctggcgctcg acggtaaaaa agcaggcggc
    28681 tattttaacc agcagattgt cgagccaatt gcgggtaaaa acggaccgct cggctatctg
    28741 cgcctgacac tcgacaccca tacgctcgcc accgaagccc aacaggtgga taacaccact
    28801 aacattttac gcctgatgtt gctgctctca ctggcaatcg gcgtagtgct gacccgcacg
    28861 cagctacagg gtaaacgcac ccgctggcag caatcgccct tcctgttaac cgccagcaaa
    28921 ccggtgccgg aagaggaaga aagcgagaaa aaagagtgac ccattactac aagaaaggaa
    28981 atcgttatgt ccacattacg cctgctcatc tctgactctt acgacccgtg gtttaacctg
    29041 gcggtggaag agtgtatttt tcgccaaatg cccgccacgc agcgcgttct gtttctctgg
    29101 cgcaatgccg acacggtagt aattggtcgc gcgcagaacc cgtggaaaga gtgtaatacc
    29161 cggcggatgg aagaagataa cgtccgcctg gcgcggcgca gtagcggtgg cggcgcggtg
    29221 ttccacgatc tcggcaatac ctgctttacc tttatggctg gcaagccgga gtacgataaa
    29281 actatctcca cgtcgattgt gctcaatgcg ctgaacgcgc tcggcgtcag cgccgaagcg
    29341 tccggacgta acgatctggt ggtgaaaacc gtcgaaggcg accgcaaagt ctcaggctcg
    29401 gcctatcgcg aaaccaaaga tcgcggcttc caccacggca ccttgctact caatgccgac
    29461 ctcagccgcc tggcaaacta tctcaatccg gataaaaaga aactggcggc gaaaggcatt
    29521 acttcagtgc gttcccgcgt gaccaacctc accgagctgc tgccggggct cacccatgag
    29581 caggtttgcg aggccataac cgaggccttt ttcgcccatt atggcgagca cgtggaagcg
    29641 gaaatcatct ccccggacaa aacgccagac ttgccaaact tcgccgaaac ctttgcccgt
    29701 cagagtagct gagagtggaa cttcggtcag gctccggcat tctcgcatct gctggatgaa
    29761 cgctttagct ggggcggcgt ggaactgcat ttcgacgttg aaaaaggcca tatcacccgc
    29821 gcccaggtat ttaccgacag cctcaacccc gcgccgctgg aagccctcgc cgggcgactg
    29881 caaggctgcc tgtaccgcgc ggatatgctg caacaagagt gcgaagcgct gttggttgac
    29941 ttcccggacc aggaaaaaga gctacgggag ttgtcgacgt ggatagcggg ggcggtaagg
    30001 taactcgctg attaaaatat ccaaggaagg aaatatgatc gatctggaaa accaggaacg
    30061 ggagattatc aatttaatgt tcagccagcg tatttcctgg ctagccgccg tgcgtatcag
    30121 gcataaatta tcgcttgctg aagtaagtaa aatgtcaggt atctcgatta attctctaaa
    30181 acagatcgaa aagacggaac ggcttagctc aaacatcaaa agcaaaatgg cagaaattta
    30241 tggttgtcca ccagagttgc tgatatgccc ttcctggatg acggctgaac ataaatgaat
    30301 agattcgtga atagatactt gaatgtattt tattttctat ccatatgaaa aataatagta
    30361 aatctataaa tcagttgcta ttgatgaata gatactaaaa agccggagca gtcccccact
    30421 ccggcttaac acgacaatcg cctttcagcg aaacacaatt actctttatc gcccagcaga
    30481 acggattcca gtgcgatttt gatcatgtcg ttgaaggtgg tctgacgctc agcggcagtg
    30541 gtctgctcgt gagtgcggat gtggtcagac acagtgcaga tggtcagggc tttcgcgcca
    30601 aactctgcag ccacgccgta gataccagcc gcttccattt ccacgccgag gatgccgtat
    30661 ttttccatca cgtcgaacat ttcgccgtcc ggagagtaga acaggtcagc ggagaacagg
    30721 ttacccacgc gagcatcaac gcccagtgct ttagctgcat ctactgcgtt acgcaccatg
    30781 tcaaagtcag cgatagcggc aaagtcatgg tctttaaaac ggatgcggtt aactttggaa
    30841 tcggtgcagg cccccatacc gataacgacg tcgcgcagtt ttacgtgcgg cagaactgcg
    30901 ccacaggaac caacgcggat aattttcttc acgccgaaat cggtgatcag ttctttggtg
    30961 tagatggagc aggacgggat acccataccg tgacccatta cggaaatttt gcggcctttg
    31021 taagtaccag tgaagcccag cataccgcga acgttgttca cttcacgggc atcttcaagg
    31081 aaagtttcag caatatactt cgcacgcagc gggtcgcctg gcatcaaaac tacgtcagcg
    31141 aaatcgccca tttctgcatt aatgtgtggg gtagccattg ttttatcctt tttgtgacat
    31201 aacaaaggcg gagtcaacgc tccgcccaaa tccatcagaa catggctttg ccatattcca
    31261 tatcagaagt accaaaatat tttgccagag tctggccgat atccgcgaag gtttcacggt
    31321 gacccagtga gcccggtttt actttcgggc catataccag taccggaatg tgttcacgcg
    31381 tgtggtcagt accggtccag gtcggatcgc aaccgtggtc agcggtgagg atcaggatgt
    31441 cgtcatcgcg cagcagagac atcagctccg gcagacggcg gtcgaacagt tccagacccg
    31501 cagcataacc ggcgacgtcg cgacggtggc cccaggaaga gtcgaagtca acgaagttgg
    31561 tgaagacgat ggtgttatca cccgcttctt tcatctcttt gatggtggcg tcaaacagcg
    31621 cgtccaagcc agtcgctttc acttttttgg tgataccgca gttggcgtag atgtctgcaa
    31681 ttttaccgac agaaaccacc tggccgtgtt tttcatcaac cagtttctgc agcacggtcg
    31741 gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt acgctggaag ttaccagctt
    31801 tgtcaccgat aaacggacga gcgataacac gaccgatatt gtagccgccg ttggtcagct
    31861 cttcacgggc gatttcgcac agttcgtaga gtttatccag accgaaagtt tcttcatggc
    31921 aggcaatctg gaacacggag tcagcggagg tatagaaaat cggcttgccg gttttcatgt
    31981 gctcttcgcc cagttgatcc agaatgaccg taccggaaga gtggcagtta ccgaggtaac
    32041 ccggcagatt agcgcgttcg accagtttat ccagcagctc ttgcgggaag ctgttttcgt
    32101 gatcggagaa atatccccac tcaaacagaa ccgggacacc ggcgatttcc cagtgaccag
    32161 acggggtatc tttaccggat gacatttcgt gcgcccatgc gtacgcgccg ataacttcag
    32221 cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc ttcgtgtgct ttcgccagcc
    32281 ccagacggat cagatttggc agattgagcg gccctttacg accattatca gcttcgcctt
    32341 tggcacaagc ttctgcgata tgacccaggg tgtcagcccc gacgtcacca aagcgttctg
    32401 catcttctgt agcgccgatg ccgaatgagt ccagcaccat aataaatgca cgtttcatat
    32461 gttctccgta ctcagtgctt caaatttaaa tgatcagatc agtataccgt tattcgctga
    32521 tacggcgata gacagttggt gtgctttccg gtgcgttatc ggcaagttta attgccgctt
    32581 tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt cgcgaggata accgccagcg
    32641 gacgctgacc gtctacctgg tcgcccagac gcgccatatc agtaaagccg acgctgtaat
    32701 cgatggtgtc agatgcctga cgacgaccgc cgcccattgc aaccactgcc atccccagcg
    32761 cgcgggtatc catttcactg acaaaacctt cggtatcagc atagactgct ttcgtcagca
    32821 tcgctgtcgg cagatacttc gcgtagttct caacgaagtc ggtcgggcct ttttgtgccg
    32881 ctaccatacg accaaagact tctgccgctt taccgttgtc cagcaccgcc tgcaatttcg
    32941 cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat cagcatctcc acgcacagca
    33001 ccatcgtgac atcaaacaga cgcgggttac gatactcacc cgtcaggaac tgcaccgctt
    33061 cacgaacttc aaccgcgtta cctgcactgg aggccagtac ctgattcatg tcggtgagca
    33121 gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac aatcgcttcg gcaagggctt
    33181 cagagagttc gtaggtcggc ataaacgcgc cgctacccac tttcacgtcc atcaccagcg
    33241 cgtccagacc ttccgccagt ttcttcgcca ggatcgaggc agtgatcagc gggatggagt
    33301 ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt atcagccgga gccagtgaac
    33361 tggtctgacc gataatcgcc acgccgacgt ctttaataat ttcgcggaaa cggttgtcat
    33421 ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc gagcgtaccg ccagtatgac
    33481 cgaggccgcg accggagatc atcggaatat agccgccgca ggctgcgacc atcggcccca
    33541 acatcagcga agtgacatcg ccgacgccgc cggtcgagtg tttatcaaca atcgggccat
    33601 tcagatgcag gcttttccag tcgagaacgg ttcctgaatc tcgcatcgcc atggtcagcg
    33661 agacacgctc aggcattgtc atatcgtgga agaaaatggt catcgcgagg gcggcaatct
    33721 gcccttcgga aatagtgttg tcgcgaatac cgttgataaa gaaacgaatt tcttcatcgc
    33781 tcagcgcatg accatcacgt tttttacgaa taatttcttg tgcgagaaac aaggtaacct
    33841 cccgaggaaa agagtatgag gcggcttttg ccggatgcgt ctacgcctta tccggcctac
    33901 gggtagatac gtctcgtagg cctgataagc gcagcgcatc aggcgtaaag catcttactt
    33961 agtagctgct ggcgctctta ccgtcgccgt ggcccagcgc tttcagcagg cttgccagca
    34021 ggctggaagc accaaagcgg tagtgacgcg catcagccca gtcagcaccg aacagttcat
    34081 ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg cacgccgccc gccggtttga
    34141 aaccaacggt tttttctacg cccatatcac ggatcacttc catcatgatg cgagcgcttt
    34201 ccggcgtcgc gttcacagcc actttaccgg tagaggtttt gatgaagtcc gcacccgctt
    34261 tgatggagat ttcagacgct ttacggatca gcgcttcgtc tttcagttcg ccggtttcga
    34321 tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc tttacaggct ttcaccaggt
    34381 caaaaccaac ctgctcgtca cccgccatca gcgcgcggta cgggaacacc acgtcaactt
    34441 catcagcacc gtaggcgatt gccgcacggg tttctgccag cgcgatttcg atgtcgtcgt
    34501 taccgtgtgg gaagttggtt accgtagcga tacggatttc cggggtgccc tgctctttca
    34561 gagttttgcg agcaatcggg ataaaacgag gatagataca gatagcggcg gtattgccga
    34621 ccggagtttt ggcctgatga cacagagcaa ttactttctc gtcggtgtcg tcgtcattca
    34681 gggtggtcag gtccatcaat ttcagtgcac gcaggctgct tgctttcaga tcagtcattt
    34741 cattctcctg gcttgtcgcc aataaaattc accttgcgag tttgttagta ttctaacatc
    34801 tactccgcaa cacacttcga tacacatcac aattaaggaa atctacttac aagtttgcat
    34861 cactgtaatg cgatctggtt caaataattc actttcaaat gaatgcgtca gtggtggcaa
    34921 acgcatcagg atcaaagtga acatcacgaa acttcttaca atggcgcatc accttcggca
    34981 taaaaggaaa agaagatgcc cacttctcat gaaaatgcac tgcaacaacg ttgccagcaa
    35041 attgtcacca gcccagtgct tagcccggag cagaagcgcc attttctggc actggaagca
    35101 gaaaacaatc tgccttaccc acagcttcct gccgaagccc gccgcgcgct ggatgaaggt
    35161 gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac cgcgctatgt cttacccgat
    35221 tacgctcgtt ttctggcgaa cggttccgaa tggctggagc tggaaggcgc gaaagatctt
    35281 gatgacgcac tctctctgct gaccattctt taccaccacg taccgtcggt cacatcgatg
    35341 ccggtctacc tggggcaact ggatgcgttg ttgcaaccgt atgttagaat tctaacacaa
    35401 aacgagatcg atgttcgaat aaaacgtttc tggcgttacc tcgacagaac cctgccagat
    35461 gcctttatgc acgccaatat cggcccgtct gattcgccca ttacccgcgc gatcttacgt
    35521 gcagacgcgc aggtttcacc gaacctgacc tttatctacg atcctgaaat cacccctgat
    35581 gacctgctgc tggaagtggc gaagaacatc tgtgaatgta gcaaaccgca catcgccaac
    35641 ggtccggtgc atgataaaat tttcacaaaa gggggctacg ggattgtgag ctgttacaac
    35701 tcactgccgc tggcgggtgg tggcagcacg ctggtacgcc ttaacctgaa agccattgcc
    35761 gagcgcagcg aatcgctgga tgacttcttt acgcgcactc taccgcacta ctgccagcag
    35821 cagatcgcca tcatcgatgc gcggtgtgaa ttcctctatc aacaatcaca cttctttgag
    35881 aatagcttcc tggtgaaaga agggctgatt aaccctgaac gttttgtgcc aatgtttggc
    35941 atgtatgggc tggcggaagc ggttaacttg ctgtgtgaga aagaagggat tgccgcgcgc
    36001 tacggtaaag aagccgccgc aaatgaagtg ggttatcgca tcagcgcgca actggcggag
    36061 tttgtcgcca atacccccgt gaaatatggc tggcaaaaac gcgccatgtt acacgcacag
    36121 tcggggatca gttccgatat cggcaccacg ccgggcgcgc gtttgccgta tggcgatgag
    36181 ccagatccga tcacccatct gcaaactgtc gccccgcatc atgcttatta ttattccggc
    36241 atcagcgaca ttctgacgct cgacgaaacc atcaaacgta acccgcaggc actggtacag
    36301 ctttgcctcg gtgcctttaa agccggaatg cgtgaattta ccgccaatgt cagcggtaac
    36361 gatctggttc gcgttaccgg ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc
    36421 gaaggttcac gcaccaacac cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt
    36481 ctggaacgcc agccgcgcgt gataagccat gaacagcaga tgcgctttag tcagtaagat
    36541 tatccccttc tcctgcgttg acgggccagg cagtcgtctg gctctgtttt tgcagggctg
    36601 caatctgcgc tgcaaaaact gtcacaatcc gtggacgatg ggacgttgca atgactgtgg
    36661 ggagtgcgtg ccacagtgtc cgcatcaggc gttgcagatt gttgacggca aagtggtgtg
    36721 gaacgctgtg gtctgcgagc aatgtgatac ctgcctgaag atgtgtccgc aacatgccac
    36781 accaatggcg caatccatga gcgtggacga agtgcttagc catgtgcgca aagcggtgct
    36841 gtttattgaa gggataacgg taagcggcgg cgaagtcacg acccaactgc cgtttgtagt
    36901 ggcgctgttt actgctatca aaaacgatcc gcaactgcgc catctcacct gtctggtgga
    36961 cagtaacggt atgttgagcg aaaccggctg ggaaaaatta ctcccggtgt gtgacggcgc
    37021 aatgctcgat ctcaaagcat ggggtagcga atgtcatcaa caattaaccg gacgcgataa
    37081 tcaacagatt aagcgcagca tctatttgct ggcagagcgc ggcaagctgg cggaactgcg
    37141 tttgctgatg atcccaggcc aggtggatta tttgcaacac atcgaagaac tggcggcgtt
    37201 tatcaaggga cttggagatg ttccggttcg cctgaacgcg tttcatgccc acgtcgtgta
    37261 tggcgaggcg caaagctggg cgagcgccac gccggaagac gttgagccgt tggctgatgc
    37321 attaaaggtg cgcggggtga gccggttgat atttccggcg ctctatttgt gacatgccgg
    37381 atacggccta cggttcggca cagacttgta ggcctgataa gacgcgtcaa gcgtcgcatc
    37441 aggcattgtg cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc
    37501 acaaacctgt aggcctgata agacgcgtta agcgtcgcat caggcattat gcgccgactg
    37561 ccggatgctg cctacgaagg gcaaactacc cacgaacgtt aaacaccgca tacgtgttat
    37621 taagcaacac ttcggcaatc tcatccgcgg gttccgggcg taactcgcaa agcacggcaa
    37681 acacacgggc agcctgttcc gggcggttag gctgcccctg aaaaccgttg agcggcatat
    37741 ccggcgcatc ggtttccagc aacaacgatg ccagcggtaa ttttgcgatg acatcgcggg
    37801 ttttactggc gcgtggatag gtgatagtac cgcctacgcc gattttgtag cccagctgta
    37861 caaaccgttc agcctgttgc tggctgccag aaaatccgtg gacaacacca gcacgcggca
    37921 aatcgtggcg tttaagatgc atcgccagtt tgtcgtgcgt gcgccgtgaa tgcaggatca
    37981 ccggcagatc gtagcgtttc gccagtttca gttgttcgtc gagtaaccac tgctgcctct
    38041 caaattgcgg atcgtcgccg aagagatcca gaccgatctc ccccaccgcc accaccttcg
    38101 ccggacgcct ttccagcgcc tgctgtagct gctcaagaga cacatcgcta tgtttttcca
    38161 acataccagg atgcaagccc aatgcggcat acagcggttg ataattttcc gctaacgcct
    38221 gcacgcgggc aaaattttcc gcctctgtcg ccggaacaat gatcttgcct acgcccgctt
    38281 gtgccgcgcg ttgcaggctg gcctcttcat cgccaataaa aggcgggaaa tcaaaatggc
    38341 agtgagtgtc gataaaacgg caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt
    38401 cgcctgcgga gcgtcgctca cttcggcaac cagcgtatcg ttggcgactg gcgcagtcgg
    38461 aatgacaatc gattccggcg tcaccactgg caccagatgg cgggtaagcg gcgctttttc
    38521 agttaatagc ttgccaaccg tggcgaggaa ataacgaccg cataaacgcc caagtttata
    38581 gtcttcacgc agcgccggaa tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg
    38641 ataaatttcg gatatccgca gcttgcccgg tggtttctca ataaattgct gaatgtcacg
    38701 atagctggtt tcatgatgct gcaccagatt gaccagcggc tgcaggctac tgtcacccag
    38761 ccagcgttcc atgcgtttga accactgcgg cgtgtagtac atttgtgacg gcacagtgcg
    38821 gatgacgacc aacgttttag cgccctgcct tgccgcctct ttaaccggaa tcgcatcact
    38881 gatcccgcca tccaggtagt taatgccttc cagcgacact ccgctacgat aaaagccagg
    38941 tatcgcactg gaggcgcgaa tcacatccag ccagttttgt ttggttggta aaaagtaatt
    39001 cggcgcgtag tcatcctgac gacaggcgca catataaaac gatttgccgc tgtcaaacaa
    39061 ccgcgcggcg gtatccattt gcaacggcat ctggcttgcg gtggcctcca ccagccagtc
    39121 gagatcgata agatttcctc cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt
    39181 ggtatagcgc atgatgactt tgcgcgcgta accgggctga ttgcagataa acgccgagag
    39241 gttctgcgcc ccggcagatg tgccgagata aagatcgaaa ggattaaact gcgcgcgcat
    39301 aaactcatcc agcacgccag ccgtgaaaat tccacgctgt ccgccgcctt cgcacaccag
    39361 agctattcgt ccaggctgga acggccttag cgacaacggc gcaatattac caagcgttac
    39421 aggtattcgc tgccccactt ctgccttcct gtttttgttg tgaaagaaag acaaagtaac
    39481 gcaatttatt attcgctaaa accgcaaaag ccagtccgct ggactggctt tggataaaat
    39541 tttcttaatg gtctgaaatc tagggtcgtt ttcggcccat gaacaaactc actaggaaca
    39601 gaataatccc gacgacaaag acaattttag ctgcgcctgc agcggtaccg gccagaccac
    39661 caaacccaag tgcggcggcg attaacgcga taaccagaaa tatgatgccc caacgaaaca
    39721 taagcgtctc ctttaccata gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa
    39781 tgcgatattt ttagtgtggt gcacatcgcg cctcccgaca aagttcggga ggacgaatta
    39841 cgacgaatta cttagttttc agatcatttt taacgctttt cacaccatct accgctttgg
    39901 cgatactttc agcacggtca ctttgtgcct gagaatctac ggtaccggag agctgaacca
    39961 cgccatcggt ggtttcaact ttcactttac gtgaagggac gatatcgtcc gccagcagtt
    40021 tggctttgat ttcactggtg gtggcggtgt cacccgcgta gtccttcacc gagccttctt
    40081 tagcgtcgcg aacgtgcagt ttgtcgctga cagaggtcac gccttcaacg cctttcgcca
    40141 ctttcactgc ctcttcggcc tgggcctgac tttcaacgaa accgctcagg gtcacgactt
    40201 tttgatcggt tttgacagag atatcggtgc tcttgatgtt gtcatgatcc accagcgccg
    40261 ccttcacttt cgcggtgatg gcgctgtcat ccatgaaatt accgacttta ttcatagagc
    40321 tatcgacttt ttgccctgcg ctttcattgg tagtctgcgc gttgttttcc gcgtaggcag
    40381 agccggtcgc gacggcagag gtcaacatta cagccagcag agttttcgaa atcttcagtc
    40441 ttgtcatagt catcgattta ttcctgtatg tttgctcgta atttgagcct agcaacacga
    40501 ggttgcattg ctgaataggg agagacttca ccctctacag aagtcaatgg tcgccatcac
    40561 aaaagcgatg ggtgatgaat aacgaccatt acagtctctg aatcagttat taatatcggt
    40621 agaatgacaa tcgacggctt tagatactga tatctacgca ttgaacggta tttaacgcca
    40681 tcagaaatgt catcactttg ttaaatatag atcacaattt tgaaaccgct cgggatatca
    40741 gcgaaaacat aagcaaaagt gaatgtttta agaacattcc gtaagcggct aataaggaag
    40801 ggaaattgac agggcgcagc ggctaccgcg ccctggaggc aagaattaat gctcgcgggt
    40861 ctggtggaac tgaacgtccg gataacgttc ctgtgccagg cgcaggttga ccatgctggt
    40921 agcgatgtaa gcgaggttat cgccgccatc aagcgccagt tggctttcgt tcttacgctt
    40981 gaactcttcg aatttcttcg cgtctgcaca ttctacccag cgggcagtgg caacgttgac
    41041 tgactcatac actgcttcaa cgttgtattc gctcttcagg cgcgctacca ccacatcaaa
    41101 ctgcagcaca ccaaccgcac caacgatcag atcgttgttg gagattggac ggaacacctg
    41161 caccgcgccc tcttcggaaa gctgtaccag ccctttgagc agctgttttt gcttcagcgg
    41221 atctttcagg cggatacgac ggaacagttc tggtgcgaag ttcggaatac cggtgaactt
    41281 catcatctca ccctgggtaa aggtgtcgcc gatctgaatg gtgccgtggt tgtgcaggcc
    41341 gaggatatcg cccggatacg cttcttcaac gtgcgaacgg tcacccgcca taaaggtcag
    41401 cgcgtcggag atcaccacat ctttcgcagt gcgcacctgg cgcagtttca tgcctttttc
    41461 atatttaccg gacaccacac gcataaacgc cacgcggtcg cggtgtttcg ggtccatgtt
    41521 ggcctgaatt ttaaatacga agccggtaaa tttatcttcg ctcgcttcta cggtacgggt
    41581 atcggtctga cgcggcatcg gcgcaggcgc ccactccacc aggccattca gcatatgatc
    41641 aacgccgaag ttacccagcg cagtaccgaa gaataccgga gtgatttcgc ccgcaaggaa
    41701 cagctctttg tcgaactcgt tagacgcgcc tttaaccagt tccagttcgt cacgcagctg
    41761 ctgtgccaga tcttcaccaa ccgcagcatc gagatccggg ttattcagcc ctttaacaat
    41821 gcggacttcc tgaatggtgt gccctttacc gctctgatag agataggttt catctttata
    41881 aaggtggtaa acgcctttaa acagcttgcc gcagccaatt ggccaggtga tcggtgcgca
    41941 gccaattttc agctcgttct caacttcatc gagcagttcc atcgggtcgc ggatatcacg
    42001 gtcaagtttg ttcataaagg tgaggatcgg cgtgtcgcgc agacgggtaa cttccatcag
    42061 cttacgggta cgatcttcaa caccttttgc ggcgtcgata accatcaggc agcagtccac
    42121 cgccgtcagg gtacgatagg tatcttccga gaagtcttcg tgccccgggg tgtcgagcag
    42181 gttaaccagg caatcgtgat acggaaactg catcacagac gtagtaatgg agatcccacg
    42241 ctgcttttcc atctccatcc agtccgactt agcgtgctgg ttggaaccac ggccttttac
    42301 tgtaccggcg gtctgaatgg cctgtccgaa cagcagcacc ttctcggtga tggtagtttt
    42361 accggcgtcc gggtgagaaa taatggcaaa agtgcggcgc ttcgccacct cttgcaaata
    42421 aggagacaac gtcataattc tttcttcttg agtaagcggc atcgcgccgc gcttgttgaa
    42481 aacgaaaaat tgcggctatt ttacccatca acggggggga ggcaatcagt gtttacacag
    42541 gagctgctcc agttcgtgca acgaagaaac ggtccaggtg ggcgcgatgc cttctggttg
    42601 ctcgcgattg tgcgcattca gccagcaggt cgcaagcccg gcgttgatgc caccgagaat
    42661 atcggactcg gcagtgtcgc caaccatcag cacgcgtgaa cggtcaggat tgcccgcctg
    42721 ttccagcgca taatcgaaaa ttttcttatt cggtttggca acgccaactt cttcggaaat
    42781 caccagcaaa tcgaagtaat cacgcaggcc cgtccgttcc agacgcactt gctgcaaggc
    42841 gctaaaaccg ttggtgatga tgccgatttt agcgttgcca cgaatggcgt taagcagaga
    42901 aaccgcgccc ggcaacggcg tacagatttc cgccatcgca ttaataaagg catcgttaag
    42961 caagcctgct tcaactttca gccgttcggc ccagctctcg aaacgcccgt gctgaagctg
    43021 taatgaagtg atcgcgccgt tttgataatc cacccacagt ggcttgttaa cggcctgata
    43081 gtcctgaaaa tcttcagcgg taaaggtgac gctgtaatca agaaacatcc gctgcaggcc
    43141 ggtgaatgag tcaaaggtaa acagcgtttc atcggcatca aagaaaatcc agtcccactt
    43201 cattattcca ccttgtctta catactgatt ggcaacgcca tgatgatggc gtcttcgcga
    43261 ccgtccgtgg tggggtagta attgcggcga atcgtcgcct cgttaaagcc taaactttcg
    43321 tacagggcaa tggcggcagc atttgaggca cggacttcca gccatagtgt cgccacgccg
    43381 cgtttttcca gttcgtcgat cagatgttcc agcagcgccc tacccagccc ctgacgctga
    43441 tagtcgggat cgaccgcaat attgaacaat gtagcttcat ccaacaccac ttgcgtaatc
    43501 gcaaacgccg ccattttgcc gttttgcgtt aactgaaagt tgagataacg ctcgccctgg
    43561 ttgctggcaa aggttttttc actccacgga aaggcgtggg cgcgttgttc aatgtggtaa
    43621 gccgctggta aatcagtcgt ttcgagggaa gaaatcgtgt tcatatgtgc aaatttgttg
    43681 ccataacgcg gcgcgtgccg ttgggtttgc ccgtaattcg gtgagcgccg gtgatgccac
    43741 ctgagcgcct tccaatgata gcggttcgtc agtacccaac cgccagctgt tgcagcgact
    43801 gccttgcggc agcatcgcga ttttttctgg cgtcagttgc agcacttgat cggggctgac
    43861 ggttaatgcg cgcagaacat cgctcactaa aggatcagtc agggcgggaa gatcgtttgc
    43921 caccatcacc agacggacgt gtgccgggct cgcaatggca atctccccct gcaacgcgcc
    43981 agggcgacgc agcgaccact gggtaatgcc cagttgctgt aactgccagt ctcgtcggga
    44041 tgtcatagcg aatcgctcct gttgtcaggg gcgcaaatat agcaaattcg tcgataccgc
    44101 gccaacatat ggctataatc gccgccagta tcaattgagg agcattccat gtctgcattt
    44161 accccggcaa gtgaagtctt gctgcgtcac agtgatgatt tcgaacaaag ccgtattctg
    44221 tttgccggag acttacagga tgacctgccc gcgcgtttag ataccgcggc cagccgtgct
    44281 catacccagc aattccacca ttggcaggtg ttaagctgcc agatggggga taacgcccgt
    44341 tttagtctgg tcgccacggc gaatgatgtc gcagattgcg atacgctgat ttactactgg
    44401 ccgaagaaca aaccggaagc ccagttccag ttgatgaatt tactttctct gctgccagtg
    44461 gggacagata tttttgtcgt tggcgagaac cgcagcggcg tgcgcagcgc tgagcagatg
    44521 ctggcagatt atgcgccgtt gaataaagtc gacagcgctc gtcgctgtgg cctctatttt
    44581 ggtcgtctgg aaaaacagcc agtatttgat gcggataaat tctggggcga atacagcgtc
    44641 gatggcctga cggtcaaaac gctgcctggc gtgtttagcc gcgacggtct ggatgtcggt
    44701 agccagttgc tgctctcgac gttaactccg cacacgaaag gtaaagtgct ggatgtcggc
    44761 tgtggcgcgg gtgtactttc agttgccttt gcgcgccatt cgccgaaaat tcgtctcacc
    44821 ttgtgcgatg tctctgcccc ggcggtagaa gccagccgcg caacacttgc agccaacggt
    44881 gttgaaggtg aagtctttgc cagcaacgtc ttttccgagg tgaaaggttg ttttgatatg
    44941 atcatctcca acccgccgtt ccacgatggg atgcaaacca gcctggatgc ggcgcaaacg
    45001 ctgattcgtg gcgcggtgcg tcatcttaat agcggcggcg agctgcgaat tgtagcgaac
    45061 gccttcctgc cttacccgga cgtgctggat gagacatttg gcttccatga agttatcgcg
    45121 caaaccgggc gcttcaaggt atatcgcgcc attatgaccc gccaggcgaa gaaaggttga
    45181 gtatctgata tctgtcgccg cataccgtgt cggatgcggc gtgaacgcct tatccgacct
    45241 acaaaacctg cacgttagcc cttcgtaggc cagataagac gcgccagcgt cgcatctggc
    45301 atctgccccc gcatgcctac caaacctgca cgctagtttc ctgatggaca tttttccagc
    45361 aattacacat caggcgataa ttaactattg acgaaaagct gaaaaccact agaatgcgcc
    45421 tccgtggtag caattctttt taagaattga tgg
//
LOCUS       DADMFL010000019        42568 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.019, whole genome shotgun sequence.
ACCESSION   DADMFL010000019 DADMFL010000000
VERSION     DADMFL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.019"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..2158
                     /locus_tag="JNZ27_001074"
                     /pseudo
     CDS             1..2158
                     /locus_tag="JNZ27_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            2149..3219
                     /locus_tag="JNZ27_001075"
     CDS             2149..3219
                     /locus_tag="JNZ27_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663494.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3231..3773
                     /locus_tag="JNZ27_001076"
     CDS             3231..3773
                     /locus_tag="JNZ27_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663495.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            3781..4296
                     /locus_tag="JNZ27_001077"
     CDS             3781..4296
                     /locus_tag="JNZ27_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8663496.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4262..5000
                     /locus_tag="JNZ27_001078"
                     /pseudo
     CDS             4262..5000
                     /locus_tag="JNZ27_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            5111..6121
                     /gene="pyrD"
                     /locus_tag="JNZ27_001079"
     CDS             5111..6121
                     /gene="pyrD"
                     /locus_tag="JNZ27_001079"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY8663497.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6295..6837
                     /gene="zapC"
                     /locus_tag="JNZ27_001080"
     CDS             6295..6837
                     /gene="zapC"
                     /locus_tag="JNZ27_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY8663498.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(6834..7943)
                     /gene="ycbX"
                     /locus_tag="JNZ27_001081"
     CDS             complement(6834..7943)
                     /gene="ycbX"
                     /locus_tag="JNZ27_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY8663499.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8187..10295
                     /gene="rlmKL"
                     /locus_tag="JNZ27_001082"
     CDS             8187..10295
                     /gene="rlmKL"
                     /locus_tag="JNZ27_001082"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY8663500.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10307..12214
                     /locus_tag="JNZ27_001083"
                     /pseudo
     CDS             10307..12214
                     /locus_tag="JNZ27_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12344..13597
                     /gene="pqiA"
                     /locus_tag="JNZ27_001084"
     CDS             12344..13597
                     /gene="pqiA"
                     /locus_tag="JNZ27_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY8663501.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13602..15242
                     /gene="pqiB"
                     /locus_tag="JNZ27_001085"
     CDS             13602..15242
                     /gene="pqiB"
                     /locus_tag="JNZ27_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY8663502.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15239..15802
                     /gene="pqiC"
                     /locus_tag="JNZ27_001086"
     CDS             15239..15802
                     /gene="pqiC"
                     /locus_tag="JNZ27_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY8663503.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16058..16225
                     /gene="rmf"
                     /locus_tag="JNZ27_001087"
     CDS             16058..16225
                     /gene="rmf"
                     /locus_tag="JNZ27_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY8663504.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16295..16813)
                     /gene="fabA"
                     /locus_tag="JNZ27_001088"
     CDS             complement(16295..16813)
                     /gene="fabA"
                     /locus_tag="JNZ27_001088"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY8663505.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(16882..18642)
                     /locus_tag="JNZ27_001089"
     CDS             complement(16882..18642)
                     /locus_tag="JNZ27_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY8663506.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18827..19278
                     /gene="matP"
                     /locus_tag="JNZ27_001090"
                     /pseudo
     CDS             18827..19278
                     /gene="matP"
                     /locus_tag="JNZ27_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
     gene            complement(19355..20401)
                     /gene="ompA"
                     /locus_tag="JNZ27_001091"
     CDS             complement(19355..20401)
                     /gene="ompA"
                     /locus_tag="JNZ27_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY8663507.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVLGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20757..21266)
                     /gene="sulA"
                     /locus_tag="JNZ27_001092"
     CDS             complement(20757..21266)
                     /gene="sulA"
                     /locus_tag="JNZ27_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY8663508.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21485..22114
                     /gene="sxy"
                     /locus_tag="JNZ27_001093"
     CDS             21485..22114
                     /gene="sxy"
                     /locus_tag="JNZ27_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY8663509.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22077..24239)
                     /gene="yccS"
                     /locus_tag="JNZ27_001094"
     CDS             complement(22077..24239)
                     /gene="yccS"
                     /locus_tag="JNZ27_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY8663510.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(24249..24695)
                     /locus_tag="JNZ27_001095"
     CDS             complement(24249..24695)
                     /locus_tag="JNZ27_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY8663511.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            24818..26872
                     /gene="helD"
                     /locus_tag="JNZ27_001096"
     CDS             24818..26872
                     /gene="helD"
                     /locus_tag="JNZ27_001096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY8663512.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFY
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(26904..27362)
                     /gene="mgsA"
                     /locus_tag="JNZ27_001097"
     CDS             complement(26904..27362)
                     /gene="mgsA"
                     /locus_tag="JNZ27_001097"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY8663513.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(27458..28120)
                     /locus_tag="JNZ27_001098"
     CDS             complement(27458..28120)
                     /locus_tag="JNZ27_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY8663514.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEAEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28293..28706
                     /locus_tag="JNZ27_001099"
     CDS             28293..28706
                     /locus_tag="JNZ27_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY8663515.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(28751..29068)
                     /gene="hspQ"
                     /locus_tag="JNZ27_001100"
     CDS             complement(28751..29068)
                     /gene="hspQ"
                     /locus_tag="JNZ27_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY8663516.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29126..30316)
                     /gene="rlmI"
                     /locus_tag="JNZ27_001101"
     CDS             complement(29126..30316)
                     /gene="rlmI"
                     /locus_tag="JNZ27_001101"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY8663517.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            30411..30689
                     /gene="yccX"
                     /locus_tag="JNZ27_001102"
     CDS             30411..30689
                     /gene="yccX"
                     /locus_tag="JNZ27_001102"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY8663518.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(30686..31015)
                     /gene="tusE"
                     /locus_tag="JNZ27_001103"
     CDS             complement(30686..31015)
                     /gene="tusE"
                     /locus_tag="JNZ27_001103"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY8663519.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31106..31765)
                     /gene="yccA"
                     /locus_tag="JNZ27_001104"
     CDS             complement(31106..31765)
                     /gene="yccA"
                     /locus_tag="JNZ27_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY8663520.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(31972..32059)
                     /locus_tag="JNZ27_001105"
     tRNA            complement(31972..32059)
                     /locus_tag="JNZ27_001105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32023..32025),aa:Ser,seq:tga)
     gene            32485..33603
                     /gene="hyaA"
                     /locus_tag="JNZ27_001106"
     CDS             32485..33603
                     /gene="hyaA"
                     /locus_tag="JNZ27_001106"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY8663521.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITKYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            33600..35358
                     /gene="hyaB"
                     /locus_tag="JNZ27_001107"
                     /pseudo
     CDS             33600..35358
                     /gene="hyaB"
                     /locus_tag="JNZ27_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            35377..36084
                     /gene="hyaC"
                     /locus_tag="JNZ27_001108"
     CDS             35377..36084
                     /gene="hyaC"
                     /locus_tag="JNZ27_001108"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY8663522.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36081..36668
                     /locus_tag="JNZ27_001109"
     CDS             36081..36668
                     /locus_tag="JNZ27_001109"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY8663523.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            36665..37063
                     /gene="hyaE"
                     /locus_tag="JNZ27_001110"
     CDS             36665..37063
                     /gene="hyaE"
                     /locus_tag="JNZ27_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY8663524.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37060..37917
                     /locus_tag="JNZ27_001111"
     CDS             37060..37917
                     /locus_tag="JNZ27_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY8663525.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38051..39595
                     /gene="appC"
                     /locus_tag="JNZ27_001112"
     CDS             38051..39595
                     /gene="appC"
                     /locus_tag="JNZ27_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY8663526.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            39607..40743
                     /gene="appB"
                     /locus_tag="JNZ27_001113"
     CDS             39607..40743
                     /gene="appB"
                     /locus_tag="JNZ27_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8663527.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            40755..40847
                     /gene="cbdX"
                     /locus_tag="JNZ27_001114"
     CDS             40755..40847
                     /gene="cbdX"
                     /locus_tag="JNZ27_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY8663528.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            40927..42225
                     /locus_tag="JNZ27_001115"
     CDS             40927..42225
                     /locus_tag="JNZ27_001115"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY8663529.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42340..>42568)
                     /locus_tag="JNZ27_001116"
     CDS             complement(42340..>42568)
                     /locus_tag="JNZ27_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663530.1"
                     /translation="LAVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNI
                     KGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10557 a        10387 c        11349 g        10275 t
ORIGIN      
        1 tttcagtacc gcaaatcgcc atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt
       61 gggatgaagg gatcaacgcg ctattactgg gatattcgtt tagcggggct aacagtattc
      121 atagcagcgc agacagtgat tctggcgaca gctattttct gaatttaaac agtggcgtta
      181 atttaggccc atggagattg cgcaacaatt caacatggag tcgcagtagt ggccaaaccg
      241 cagaatggaa gaatctcagc agctatttgc agcgggcggt tattccacta aaaggcgaac
      301 tgaccgtagg tgatgattat actgcaggcg attttttcga tagtgtcagc tttcgtggtg
      361 tgcagctggc gtcagatgac aacatgctgc cagacagcct gaaagggttt gcgcctgtgg
      421 tgcgtggtat cgccaaaagc aatgcccaga taacgattaa gcaaaatggt tacaccattt
      481 accaaactta tgtatcgcct ggtgcttttg aaattagtga tctctattcc acgtcgtcga
      541 gcggtgattt gttagttgaa atcaaagaag cggacggcag cgtcaatagc tacagcgtac
      601 cgttttccag cgtgccatta ctccagcgtc aggggcgaat caaatacgcg gtgacactgg
      661 cgaaatacag aaccaatagt aatgaacagc aggagagcaa atttgcccag gccacgttgc
      721 agtggggcgg accgtgggga acgacatggt atggtggtgg acaatatgct gaatattacc
      781 gtgccgccat gtttggtctg ggatttaacc ttggcgattt cggagcaatt tcgttcgatg
      841 cgacccaggc gaagagtacg ctggcagagc aaagcgaaca taaaggtcag tcatatcgtt
      901 ttctgtatgc caaaacgctc aaccaattgg gcactaattt tcaattgatg ggctatcgct
      961 attcgacgtc gggtttctac accctttccg acaccatgta taaacatatg gatggctacg
     1021 aatttaatga cggtgatgat gaagatacgc cgatgtggtc gcgttattac aatttgtttt
     1081 acaccaaacg tggcaaactg caggtcaata tctcccagca attaggcgag tacggttcgt
     1141 tttatttaag tggtagccag caaacttgct ggcataccga tcaacaggat cggctattac
     1201 agtttggcta caacacgcaa attaaagatc tctcgctggg ggtttcctgg aactacagta
     1261 agtcccgtgg tcaacctgat gctgatcagg tgtttgcact aaatttttcc ctgccgctca
     1321 atctgttgct ccccagaagt aatgatagct ataccaggaa aaaaattacg cctggatgac
     1381 ctctaacacc agtatcgata acgaagggca cattacacaa aacctgggtt taacggagac
     1441 actactcgat gacggtaacc tgagctacag cgtgcaacag ggatataaca gcgaggggaa
     1501 aacggctaat ggtagcgcca gcatggacta caaaggggcg tttgcagatg cccgagtggg
     1561 ctacaactac agcgataacg gcagtcaaca acaactgaac tacgctcttt caggcagttt
     1621 agttgcccat tcacagggca ttaccctggg gcaatcgctg ggggaaacta acgtgctgat
     1681 tgcagcacca ggcgcagaga atactcgtgt ggcgaacagc accgggctga aaactgactg
     1741 gcgcggatat accgttgtcc cctatgccac ttcttatcgg gaaaatcgaa tcgcacttga
     1801 tgcggcatcg ttaaaacgta acgtggatct tgaaaatgca gtagtcaacg tggttcccac
     1861 caaaggggcg ttggttctgg cggagttcaa tgcccatgcg ggagcaaggg tattaatgaa
     1921 aacatcaaag cagggtatac cgctgcgttt tggcgcgata gcgacgctgg acggcataca
     1981 gactaatagc ggcattattg atgatgatgg ctcgctctat atgtccggtt tgcccgctca
     2041 gggagcgata actgtacgct ggggcgaagc tcccgatcaa atttgtcata tcagttacca
     2101 gcttaccgaa caacaaatta actctgcgat tacgcgaatg gatgccatat gcagataatc
     2161 tttggagaaa aatgcgtgtt attactacga ctattttttg ccgccgtctt aatgctatgg
     2221 tgcgctcaaa ctgctgctta tatcgggcag tgtcatacca ctcaggggaa tccgtatatt
     2281 ggcgtcaatt ttggcgttaa aactctggat gaagaagaaa atacggctgg agtagttaaa
     2341 gacaaatttt atcagtggaa cgaatcgagt gattattatg tttcctgtga ttgcgataaa
     2401 gacaatgtca gaagtggccg atgggcattc gccgcggatt caccgttagt ctatttaggc
     2461 gacaactggt acaaaattaa tgactatctt gccgccaaag ttttattgca ggttaaaggc
     2521 agttctccta ctgcggttcc tttcgaaaac gtgggcacag gggcagatac acgatggcat
     2581 atttgcgatc ccggcggtca acgtttaggt ggccaggggg ctagcggtaa tagcggtagc
     2641 ttttccctga aaatattgca gccgttcgtt ggttcggtcg tcattcctcc tatggcgctg
     2701 gcgcgattat ttgaatgcta caacataccc gcaggtgatt cctgcacgac tactggtaca
     2761 ccggttttag tgtattacct gtctggtacg atcaattcac ttggctcatg ttccgtcaat
     2821 gccggagaaa caatcgaggt tgatctgggc gacgtatttg cggctaactt tcgtgttgta
     2881 gggcataagc ctcttggggc cagaacggca gaacttgcaa ttccggtcag gtgtaacacg
     2941 ggaaacgcag ggttagttaa cgtcaatctg agtctgacgg caacctcaga ccccagctat
     3001 ccccaggcga ttaagacgtc acgtcctggc gtgggcgtgg tggtgaccga tagccagaac
     3061 aacattattt cccctgctgg tgggacacta ccgctctcta ttcctgatga tgcagacagt
     3121 atcgcgcgaa tgaatgtcta tccagtcagc acgacaggcg taccaccaga aaccgggcga
     3181 tttgaagcca cggcaacggt gagaataaat tttgattaac gcgtgaacgt atgaagaaaa
     3241 aaacgatata tcagtgcgtt attttgttct ttagccttct taacatccat gtcgggatgg
     3301 ctgggcctga acaagtcagt atgcatattt atgggaatgt ggtcgatcag ggctgtgatg
     3361 tcgccaccaa aagtgcatta caaaatattc atattggtga ttttaatatc agtgattttc
     3421 aggccgcgaa taccgtaagc actgctgctg atttgaatat tgatatcacc ggttgtgccg
     3481 ctggtattac tggcgcggac gtccttttta gcggcgaggc tgacaccctt gcgccgacac
     3541 tgctcaaact aactgacaca ggcggaagcg gtggtatggc aacggggatt gccgtgcaaa
     3601 ttcttgatgc gcaaagtcag caagaaatcc cgctcaatca ggtccagcct cttacgccct
     3661 taaaagccgg ggataacaca ctcaaatatc aacttcgtta taagtccaca aaggcgggag
     3721 caacgggcgg taatgcgacg gcggttctct attttgatct ggtttaccag tgaaggagtg
     3781 atgttgaaac ggataatctg gattctgttc ttactgggat taacgggggg ctgtgagcta
     3841 tttgcccatg atggcacggt caacattagc ggatcgtttc gccgtaatac atgcgtgctg
     3901 gcacaggata gcaagcaaat caatgtgcag ttaggcgatg tctcgctgac acgttttttt
     3961 catgggaatt atggcccgga gaaatctttc atcattaatt tgcaggattg cggaacagat
     4021 gtcagcacgg ttgatgtcac cttttcagga acccccgacg gagtgcagag cgaaatgctg
     4081 tccattgaaa gtggtacgga tgcagccagt gggctggcaa ttgcgattct ggatgatgcg
     4141 aagatattga ttccgcttaa ccaggcgagt aaagactaca gcctacatag cggtaaggtg
     4201 ccattaactt tttatgcgca attgcgacct gtcaatagcg atgtgcagtc gggtaaagtg
     4261 aatgccagcg cgacatttgt acttcattat gactaatacg tggaacagat tagcgctctt
     4321 gattttcgcc gttttatcgc tgctggtggc gggtgaattg caggcaggag tggtggtcgg
     4381 cggaacgcga tttatctttc cggcagacag agaatcgata tctattttac tgactaatac
     4441 cagtcaggaa tcttggctta ttaacagcaa aatcaatcgc ccaacgcgtt gggccggggg
     4501 gggaagcgtc gacagtgcca gcactattac tggccgctcc gccacttatt ctcctgaagc
     4561 ccggtactac aggcacgttg cgcttgctga gaacggaaag cgacatcttg cctgtggatc
     4621 gcgaaacgct atttgagtta agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga
     4681 gcgtaaaagt ggcgatgcgc tcggtattta aactgttctg gcgacacgaa ggtttgccgg
     4741 gcgacccgct ggaagcttat caacaattac gctggacacg gaattcgcag ggtgtacaac
     4801 tcactaaccc aacgccttac tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt
     4861 taagtaatgc gggagtcgtg ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa
     4921 tagcaccgtg tcatgtcgcc tggcggacga taaatgatta cggcgggttg agtgcaaaga
     4981 aggagcaaaa tctgccctga aacaggttct gaaaacgttt gcgttttttt tgccgcaggt
     5041 caattccctt ttggtccgga ctcgcacata atacgccccc ggtttgcaca ccgggaatcc
     5101 aggagagttc atgtactacc ccttcgttcg taaagccctt ttccagctcg atccagagcg
     5161 cgctcatgag tttacttttc agcaattacg ccgcattacc ggaacaccgt ttgaagcact
     5221 ggtgcggcag aaagtgcctg cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc
     5281 gcttggtctg gcagccggtc ttgataaaga cggggagtgc attgatgcgt taggcgcgat
     5341 gggatttgga tcgatcgaga tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa
     5401 gccgcgtctc tttcgtctgg tagatgccga aggtttgatc aaccgtatgg gctttaataa
     5461 tcttggcgtt gataacctcg tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg
     5521 tattaatatc ggcaaaaata aagatacgcc agtggagcag ggcaaagatg actatctgat
     5581 ttgtatggaa aaaatctatg cctatgcggg atatatcgcc atcaatattt catcgccgaa
     5641 taccccagga ttacgcacgc tgcaatatgg tgaagcgctg gatgatctct taaccgcgat
     5701 taaaaataag caaaatgatt tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa
     5761 gatcgcgccg gatctttctg aagacgaatt gatccaggtt gccgatagtt tagttcgcca
     5821 taatattgat ggcgttattg caaccaatac cactctcgat cgttctctgg ttcagggaat
     5881 gaaaaattgc gatcaaaccg gtggtttaag tggtcgtccg cttcagttaa aaagcacaga
     5941 aattattcgc cgcttgtcac tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg
     6001 catcgactcg gttatcgctg cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat
     6061 ttattctggt tttattttta aaggtccgcc gctgattaaa gaaatcgtta cccatatcta
     6121 attatttctt cgaattcctc tttataagat aatcagggct ttattttcag ccctggttgt
     6181 tttatattca tcgctgttgc ttatttagac attttgtact tttattgttg aggttattaa
     6241 gcgaagcaac aatggattgt gttgctgcgg ttttataggg taggggagag gcagatgcga
     6301 attaaaccag acgataactg gcgttggtat tacgatgaag agcacgatcg aatgatgctc
     6361 gatttagcca atggtatgct atttcgctca cgttttgcgc gcaaaatgtt gactccagat
     6421 gccttttctc ccgcaggctt ttgcgttgat gatgccgcgc tctatttctc ttttgaagaa
     6481 aagtgccgcg attttaattt atctaaagac caaaaagccg aactggtgct gaatgcactg
     6541 gtagcaattc gttatctcaa accgcaaatg ccgaaaagct ggcattttgt ctcccatggt
     6601 gaaatgtggg tgcccatgcc gggcgatgcg gcctgcgttt ggttgagcga tactcacgag
     6661 caggtcaatt tgctggtcgt tgaatctggc gaaaatgccg cgctatgcct gctggcacaa
     6721 ccctgcgttg ttattgcggg tcgcgcgatg cagttgggcg acgccattaa aatcatgaac
     6781 gacaggctga aaccgcaggt gaatgttgac agcttcagcc tcgaacaggc agtctaacgc
     6841 gtcagcttaa gggcagtctt cggtacacag ctacagcaaa gaatggtgcc atcatcgccc
     6901 attgctgatt ttttcagcgg cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca
     6961 caaatgcccg cgcggcaaga ataagggata cgaattccct gattttctaa ttgctccagc
     7021 aacacttgtt ggttatttcc acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg
     7081 tccggttgtt gcgtgatgtt ggcggtatca tcagcggcag ctgcgccgta aatttttgcc
     7141 ggagccgttg ccagaatttc cacctcatcg ccaacgcgaa tcacgccgct attacgggca
     7201 attaaattct gaccaaaatc gacatcgccg ttatcctggg cagtgcggaa agattgcaat
     7261 gtttttaatg gttcgcctgc cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa
     7321 atacagcggc tacaaggttt aaccacatca aacaccacat caccaatgcg aatcactttc
     7381 cagctatctt cttcccaggc tgacgcgccg gaaaccacca gattggggcg gaattgctcc
     7441 atttttacac tggccggaca acgttgttgg agatcacgta acgaggcttc gttagcaaga
     7501 aggtaaggat agccatcagc aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg
     7561 gtcatttgtg gccccaccca gcgtaattgc acttcgcggg agaaaaatcc acttagccat
     7621 ttgttgatcg cgtctggcgc aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt
     7681 gcgtcttgtg tggcgaaatc agcaaaacga acatatgcgc tactgccatc tggtgcggtg
     7741 agatgcaagc catcatgcac aggtgaagga gtaaaccgta ccatctgggg aaactggcga
     7801 gcggtaataa acgtaccgtc aggttccgtg atcataaaga tgcgatcgaa ggccagacca
     7861 ctgacatcag ccagagcatg tgtaagacca atgccgcgca tcgatttaac aggatgaata
     7921 aaaagccggg ttaatgtcgc cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta
     7981 tgacatgacg cgcttattag ctataatgcg caacaatttt cttagcgcct gtcccgttag
     8041 gctattttat ttgtcagttt ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac
     8101 gctccggttt ctccgcgctc tccatgtcca gactgactgc aacaattacg cctactgcgg
     8161 taggctctta gagtaaaagt gacgatatga attctctgtt tgccagtacg gcccgtgggc
     8221 tggaagagct gttaaaaact gaactggaaa atctgggggc cgttgaatgc caggtggttc
     8281 agggtggggt ccatttcaag ggcgacacac ggcttgttta ccagagcctg atgtggagcc
     8341 gcctggcctc gcgtattatg ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc
     8401 tctatctcgg tgttcaggcg atcaactgga cagagatgtt taatcctagc gcgaccttcg
     8461 ctgtccactt cagtggtttg aatgacacca tccgcaacag tcagtacggc gcgatgaaag
     8521 tgaaagacgc gatcgtcgat gctttcacgc ggaaaaatct gccgcgtcca aatgttgatc
     8581 gcgatgcgcc ggatatccgc gttaacgtct ggctgcataa agaaaccgcc agtatcgctc
     8641 ttgatctgag tggtgatggt ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc
     8701 cgatcaaaga aaccctggca gccgctattg tgatgcgatc cggctggcag tcaggaacac
     8761 cgctgctcga tccgatgtgt ggttccggta cgttgctgat tgaagcggcg atgctggcga
     8821 ccgatcgcgc accaggcttg caccgtgggc gttggggctt tagcggctgg gcgcagcatg
     8881 atgaagctat ctggcaggaa gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctgg
     8941 ctgagtatag ctctcatttt tacggttcgg acagcgacgc acgggtgatt caacgtgcgc
     9001 gcactaacgc ccgtcttgcg gggattggtg aactgatcac ctttgaggtg aaagatgtcg
     9061 cgcaactgac caatccgctg ccgaaagggc cgtacggtac agtgttgagc aacccgccat
     9121 acggtgaacg tctggacagc gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa
     9181 tcatgaaaaa ccagttcggt ggctggaatc tctctttgtt tagtgcctcg ccggatctgc
     9241 taagctgcct gcagctgcgt gcagacaaac agtacaaggc gaaaaacggc ccgctggact
     9301 gcgtacagaa aaattaccac gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag
     9361 cggaagatta cgccaaccgc ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc
     9421 aggaagggat tgaatgttac cgcctgtatg acgccgatct gccggaatat aacgttgctg
     9481 ttgaccgtta tgccgactgg gtggtggtgc aggagtatgc gccgccaaaa actattgatg
     9541 cgcacaaagc gcgtcagcgt ctgttcgata ttatcgctgc aaccatttcg gtactgggga
     9601 ttgcgccaaa caaactggtg ctgaaaaccc gtgaacgcca gaagggcaaa aaccagtacc
     9661 agaaactggg cgagaagggc gagtttcttg aagttaccga atataacgct cacttgtggg
     9721 tgaacctgac ggattatctc gatactggtc tgttccttga tcaccgtatc gcccgccgta
     9781 tgctcggtca gatgagcaaa ggcaaagatt tcctcaacct gttttcttat accggcagcg
     9841 ccaccgtgca tgcgggatta ggcggtgcac gcagcaccac caccgtggat atgtcgcgta
     9901 cttatctgga atgggcagaa cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc
     9961 gcctgattca ggccgattgc ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga
    10021 tctttatcga tccgccaacc ttctctaact caaaacgaat ggaagatgcg tttgatgttc
    10081 agcgcgatca tctggcgctg atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga
    10141 tcatgttctc gaacaacaaa cgtggcttcc gtatggatct cgacggcctg gcaaaactgg
    10201 gactgaaagc acaagaaatt acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc
    10261 agatccacaa ctgctggctg attaccgcag cctgaaagga atagtaatgt cattaatcag
    10321 tatgcatggc gcatggctgt cgttcagcga cgcgccgctt ctcgataacg cagaactgca
    10381 tatcgaagat aacgaacgtg tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt
    10441 aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt cgcattattt atgagcaaga
    10501 tttgattgta gctcgtctgc aacaggatcc gccgcgtaac gttgagggta gcgtttatga
    10561 tttcgttgcc gaaggcattg aagaacaagc ggaatatctg aaacgctatc acgatatttc
    10621 gtgcctggtg atgaacgacc cgagcgagaa aaatctcaac gaactggcga aggttcagga
    10681 acagctggat caccacaacc tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca
    10741 actggggtta gatcctaacg ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc
    10801 ggcattagga cgcgcgctgg tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa
    10861 ccatctggat atcgaaacca tcgactggct ggaggggttc cttaaaacct tcaatgggac
    10921 gattattttc atctcccacg accgttcgtt tatccgcaat atggcgacgc gcattgttga
    10981 tctcgatcgc ggcaagctgg tgacctatcc agggaattac gaccagtacc tgcaggaaaa
    11041 agaagaagcc ctgcgcgtgg aagaactaca aaatgccgag ttcgatctca aactggcgca
    11101 ggaagaagtg tggattcgct aggggatcaa agcacgccgt acccgtaatg aaggccgcgt
    11161 acgcgccctg aaagcgatgc gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc
    11221 aaagatgcag gtggaagagg ccagccgctc cggtaagatc gttttcgaaa tggaagacgt
    11281 ttgctaccag gttaacggta agcaactggt gaaagatttt tctgcccagg ttctacgtgg
    11341 cgacaaaatt gccctgattg gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat
    11401 gctcggtcag cttcaagcgg gcagcgggcg tattcacgtt ggcaccaaac tggaagtggc
    11461 ttatttcgat cagcaccgcg cggaactgga tcccgataaa acggtgatgg ataaccttgc
    11521 cgaaggtaag caagaggtga tggttaatgg caagccacgc cacgtattgg gctatttgca
    11581 ggactttctg ttccatccga aacgggcgat gacgccagta cgtgcgcttt ctggcggtga
    11641 gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca agcaacttat tgattcttga
    11701 cgaaccgacc aacgatcttg atgtcgaaac gctggaactg ctggaagaac tgatcgacag
    11761 ctatcagggc acggtattgc tggttagcca cgatcgtcag tttgtcgata acaccgttac
    11821 agaatgctgg atcttcgaag gcggcggtaa aattggtcgt tatgttggcg gttatcatga
    11881 tgcccgtggt cagcaagaac aatatgtggc actcaaacag cctgcggtga aaaaaagcga
    11941 agaagccgcc gcgccaaaag cagaaactgt aaaacgcagc agtagcaaac taagctataa
    12001 attgcagcgc gaactggagc agctaccgca attgctcgaa gatctggagg cgaagctgga
    12061 agccctacag acgcaagtgg cggatgcttc cttcttcagt cagccgcatg agcagacgca
    12121 aaaagtgctt gctgatatgg ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg
    12181 ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag
    12241 acagctgaca acggtaaaat tgcctgatga actgggttta tcaggcatga aaaccaacac
    12301 tttgttagtc atctgtaaaa tagcgcatca ttaaggagta ccaatgtgcg aacatcatca
    12361 tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg gtggcgttac cgcgccttga
    12421 gcatggtcag aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc
    12481 ccctcggcag cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa
    12541 cttgtttcct tttgtgaata tgaacgttgc aggagttacc agtgaaatta cattactgga
    12601 aattcccggc gtgctttttt ctgaggacta cgccagcctc ggcacctttt tcctgttgtt
    12661 tgtgcaactg gttcccgcgt tttgtctgat aaccattctg ttactggtga atcgcgcgga
    12721 attaccggtc cgtttaaaag agcaactggc acgggtgctt tttcaactca aaacctgggg
    12781 aatggcggag attttcctcg cgggtgtgct ggtcagtttc gttaaactga tggcttacgg
    12841 cagcattggg gtaggcagca gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg
    12901 cgcttttcag tgcgttgatc gtcgctggtt atgggacgac atcgccccga tgccagaact
    12961 gcgccagctg ctaaaaccag gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg
    13021 ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta
    13081 cgttcggcgt agaaacagcc tgcagtggac actcgcgctg cttgtaacgt ccatcatgct
    13141 gtatcttccg gctaatattt tgcccatcat ggtgacggat ttattaggct cgaagatgcc
    13201 gtcgacgatt ctcgctgggg tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc
    13261 ggtgatcttt ctggccagta ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct
    13321 gtgttgggat gccaaagggc atggcaagcg cgacagtgaa agaatgcatt tgatttatga
    13381 agttgttgag tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg cggtgctctc
    13441 ggcgctggtg cgtatgggag gtttaatgag tatttatccg gcaatgggtg cattaatgtt
    13501 tgctttagtc gtcataatga caatgttttc tgctatgacg tttgacccgc gtttgtcgtg
    13561 ggatcgtcaa cctgaatcag agcatgagga gtcctgacag tatggaatct aataatgggg
    13621 aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg gatatttcct atcgtcacgg
    13681 cgctcattgg ggcctgggtt cttttttatc attacagcca tcagggaccg gaagtgaccc
    13741 tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac caccattaaa agccgtagcg
    13801 ttgacgtcgg cgtggttgaa agcgccacac tggctgatga tttgacgcac gttgaaatca
    13861 aagcgcggct gaattccggt atggaaaaat tgctgcataa agacaccgtc ttttgggtgg
    13921 tgaaaccgca gattggtcgc gaagggatta gcggcctggg aacgctgctg tctggagttt
    13981 atatcgaact gcagccaggc gcgaaaggca gcaaaatgga taaatacgat ttgctggact
    14041 caccaccgtt ggccccgcct gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag
    14101 ccgggcagct ctcgccagga gatccggtgc tgttccgtgg ctatcgggta ggttcggttg
    14161 aaaccagcac cttcgatacg caaaaacgca atatcagtta tcaactgttc atcaatgcac
    14221 cttatgaccg actggtgacc agcaatgttc gcttctggaa agatagtggc attgcggttg
    14281 atctgacgtc agcggggatg cgtgtggaga tgggctcatt gacaacgctg cttagtggcg
    14341 gtgttagctt tgatgtgccg gaaggtctgg atttagggca gccagtggca ccgaaaacag
    14401 ctttcgtttt gtatgatgat cagaagagca ttcaggattc gttgtacacc gatcacattg
    14461 attatctgat gttctttaaa gattcggtac gcggtctgca accgggagct ccggtagaat
    14521 tccggggtat tcgcctgggt accgtaagca aagtgccatt ctttgcgccg aatatgcgtc
    14581 agacatttaa cgatgattac cgtattccgg tactgattcg tatcgagcca gagcggctga
    14641 aaatgcagct tggcgaaaat gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg
    14701 gtttacgcgg atcgctgaaa accggaaacc tggtcactgg tgcactgtat gttgatctcg
    14761 atttctatcc aaatacgcct gcaataaccg gtattcgtga atttaatggt tatcagatta
    14821 tcccgactgt tagcggcggc ctggcgcaaa tccagcaacg actgatggaa gcgttggata
    14881 agatcaacaa actgccattg aatccgatga ttgaacaggc aaccagtacg ctttctgaaa
    14941 gtcagcgcac aatgaaaaac ctgcaaacga cgctggatag catgaacaag atcctcgcca
    15001 gccagtcgat gcagcagttg ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca
    15061 gcatgcaggg cttccagcct ggctccgcag cctacaacaa gatggtggcg gatatgcagc
    15121 gccttgatca ggtgttgcga gaactgcaac cggtgctgaa aacgctcaat gagaagagta
    15181 acgcgctggt atttgaagcg aaggacaaaa aagatccaga gccgaagagg gcgaaacaat
    15241 gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc ggatgcagct ccggcgaaat
    15301 taataaaaac tattaccagt tacctgtggt gcagagcggt acacaaagta ccgccagcca
    15361 gggcaatcgt ctgttatggg tagagcaggt cactgttcct gactatctgg cggggaatgg
    15421 tgtggtttat caaaccagtg atgtgaagta tgtgattgcc aacaacaact tgtgggccag
    15481 cccgttggat caacagttgc gcaacaccct ggttgccaac ctgagtacgc aactgcccgg
    15541 ctgggtggtt gcctcccagc ctctgggaag cgcccaggac acgctcaatg ttaccgtaac
    15601 ggagtttaac ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc tgttgaacca
    15661 ccagggacaa ctgatcaaac gtccgttccg tctggaagga gtgcaaactc aggatggtta
    15721 cgatgagatg gttaaagtgc tggccggtgt ctggagtcag gaagccgctt ctattgcaca
    15781 agagataaag cgtctacctt aattataaag atttgtaaat ataaccgtct ccggtatgtt
    15841 gcctgaggcg gtttttttgt ttctaacgtg cggaaaaatt tgttcctctt cacatttttt
    15901 gtacaaccga catgcccgtg tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg
    15961 acggcagtta tgattcgcgg tattgcttaa ctgtgattgc acatttagta atcactgttt
    16021 tcttttccac cagaaaccag tatgagggaa acgaggcatg aagagacaaa aacgagatcg
    16081 cctggaacgg gcacatcaac gtggttatca ggccggcatc gccggacgct caaaagaaat
    16141 gtgtccctat cagacgctga atcaaaggtc acaatggctg ggaggctggc gagaagccat
    16201 ggcggacagg gtagtaatgg cctgattctg tctctttaaa aagaaacctc cgcattgcgg
    16261 aggtttcgcc ttttgatact ctgtctgatg ataatcagaa ggcagacgta tcctggaaca
    16321 gaccgacttt caggtcgctg gcggtataga tcagacgaca atcaaccagc acttcgccat
    16381 ctgccagacc cataatcaga cgacgattaa caatgcgttt aaagtgaata cggtaggtca
    16441 cttttttcgc tgtcggcagt acctgaccag tgaatttcac ttcgccaacg cccagcgcgc
    16501 ggcctttacc ttcgccgccc agccagccga ggtagaaccc taccagctgc cacattgcgt
    16561 ccaggcccag gcatcccggc ataaccggat cgccaatgaa gtggcatccg aagaaccaca
    16621 gatccggatt gatatccagt tctgcttcaa catacccttt gtcgaagtta ccacccgttt
    16681 cggtcatttt gaccacacgg tctatcatca gcatgttcgg tgctggcaat tgcgggcctt
    16741 tagcgccaaa cagttcaccg tgaccagagg caagaaggtc ttcttttgta taggattcgc
    16801 gtttatctac catgttctct gtaagcctta ttttattgaa gcacgcagga tagctaacac
    16861 gtgtacgctg aacaagtccg atcagttcgg aataaaccag ttcagccaac gtaatggcca
    16921 tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg atacgttcct ggatggtttg
    16981 catcagcgtc gtttggcctt cgccatccca caccagattt aataataacg gcagtgcgtc
    17041 agtcacatcg tctactgccc agatggtgaa tttgccttct tctaccgctt tcaccagttc
    17101 actgtgaaga cttaaatggc gaacgttagc agtggggata atgacacctt gtttccccgt
    17161 taactcacgt tgctggcaaa tagcaaagaa gccttcgatt ttctcattta aaccaccgac
    17221 cggctggacg cggccgaact gatcgactga acctgtgata gcgatactct gattcactgg
    17281 cacatcagcg agggcgctta tcagggcgca gagttcagcc atcgaggcac tatcgccatc
    17341 cacttcactg tatgactgct caaatgtcag cgatgctgag aaggggatct gttgctcaag
    17401 ctgtagttcc gacatcagga acgcctgcat gatcatcatc cctttcgcat ggatattgcc
    17461 gccaagctcc gctttgcgtt cgatgtcggt gaattcacca tcgccaatat gcacaacgca
    17521 gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag
    17581 ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc aggatttgct cctgaaggat
    17641 ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa
    17701 gtttagctgc tcgccggaga aggtgtcgcc atcacacagg gaggcgacct ctttacactg
    17761 gcggaggatc caatgcgggc taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc
    17821 ttcgcggata agtaccggcc aagcatccgc tcccggggca ggtaagtgat tatgtctggc
    17881 ggtaaatgtc acccagcgac accactggct tactgactcc gcatcgacaa tctgcagagt
    17941 atcttcaaat tcgctataaa tagcctgctc tgaaagctct ggctccatct cctggaaatc
    18001 agccaatgat tcgcgttcgc ctaccagaat gactttcagc ttcaatggca tcgaaggcac
    18061 agagacgggg agagggcgcg actcatcaaa cgcaacccag tcaaaacgct cgcggttaac
    18121 gatatttttc agccgcatcc acagcagagg ttgcgccagc agtgtacgca aagagatgat
    18181 gagaataccg ccatttgcct gatgcaccag accaggctgc agggtaatgt cgccattaaa
    18241 ctggcgcagg cagccaaaga gttgctccgc ttctacccag tcggcagcga caacttgcgt
    18301 taaagtcgca aaattatcat ctgcactcac tgcgtgacgt aagcggatgg agtggccgga
    18361 aacctcatag tgatcgccca ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc
    18421 ggcaataaga ttcagatact cagactcttc cggggcctta gccagcataa aagaggagga
    18481 tgctcgcgta tgcagcaact gctccagcgc aaattgcagt cgcggttgag tatcactgaa
    18541 taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat agctatcggt
    18601 atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg ttttttagtc
    18661 tgttgtcaaa gccgcgatta taccgtaacc ggcactacag cacacgtaga aagcaccgac
    18721 aatactcctg gctgggcgtt aaagctcaca ggatggagat tcttttcttc actggcctaa
    18781 aaagctgata ttctgtaaag agttacacgg taacattgag atcgctatga aatatcaaca
    18841 acttgaaaat cttgaaagcg gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg
    18901 ggagttaatt acccgttaca tagaagccag tgccgcccag gaagccgtcg atgtgttgct
    18961 ctcgctcgaa aatgaacccg tgctggtaaa tggctggatt gacaagcata tgaatccgga
    19021 actggtcaat cgcatgaagc agaccattcg ggccagacgg aagcgccatt ttaatgcgga
    19081 acaccagcat acgcgcaaaa aatcgatcga tctggaattt attgtctggc aacgtctggc
    19141 tggtcttgca cagcgtcgcg gtaaaacgct ttctgaaacg attgttcagc tgattgaaga
    19201 tgcggaaaac aaagagaaat acgcgaataa atgtcttctc tgaagcagga tctgcaggca
    19261 ttgctgggta aggaataact gacgaaagtc agttcaattt actaaaggca aaaaaaaacc
    19321 ccgccgcagc ggggtttttc taccagacga taacttaagc ctgcggctga gttacaacgt
    19381 ctttgatacc tttaacttcg atctctacgc gacgatccgg agccaagcag tcgatcagtg
    19441 cagcacgctg tttcacgttg tcacaggtgt tgccagtaac cgggttggat tcgcccatac
    19501 cacgtgcgga gatcttgtct gccgggatac ctttggagat caggtaatca acaacagact
    19561 gagcacggcg ctcggacaga ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac
    19621 ccagaacaac tacggaaccg tctttcggat ccaggttgct cagctggctg tacagctgat
    19681 ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag
    19741 acttcagagt gaagtgcttg gtctgtactt ccggtgccgg agctggagca actaccggag
    19801 ctgcttcgcc ctgaccgaaa cggtaggaaa cacccaggct cagcagaccg ttgtccggac
    19861 gagtaccgat ggtgtttgcg tcaccgatgt tgttggtcca ctggtattcc agacgggtag
    19921 cgatttcagg agtgatcgcg tactcaacac cacctgcgaa aaccggagaa acgccagtgt
    19981 cgtggtcttt aaaggatgcg ccaccaagta cgttagcctt ggtgtctgca cgccatacca
    20041 taccacccag acgagtgtag atgtccagat cgtcagtgat tgggtaaccc agtttagcgg
    20101 tcagctgaac gccctgagct ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac
    20161 gacctaacca gtcgtaaccc atttcaaaac caacatacgg gttaacctgg taaccaccaa
    20221 aagcacctgc acccagttgg ttttcgtggg tcggaccatt gttaggaata aaaccagtgt
    20281 catggtactg ggaccagccc agtttagcac cagtgtacca ggtgttatct ttcggagcgg
    20341 cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct gtctttttca
    20401 ttttttgcgc ctcgttatca tccaaaatac gccatgaata tctccaacga gataacacgg
    20461 ttaaatcctt caccggggga tctgctcaat attaactcta ccgatatctt cggcttatgc
    20521 cgagcacccc tagcgatgta aagtctacaa cgtagttgaa aacttacaag tgtgaactcc
    20581 gtcaggcata tgaaaaaaaa gtcttgtata aggtatgttt aatctttttg tcagcgacaa
    20641 tttacagaaa aaaaacgcgg aaaccgcttc agacaagcct tcgcaaggaa aattagtcac
    20701 gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa atttacttaa
    20761 tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg
    20821 cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca
    20881 gcatgctctt cttcagtcaa atcatctgtc aaccaaccga tcaccacact gtaattgccc
    20941 gtgcgtaaag cgcgaaccat tgactccact gtgtggcaag gggagagctg gctaatctgc
    21001 attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc
    21061 ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt
    21121 agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct
    21181 gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa
    21241 cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat
    21301 gtgcagtaca tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt
    21361 ttgtcattgg tgacgggaaa acataaatta atcttgcccc ttaagaataa gttgcctatt
    21421 ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt ttaaaaggaa
    21481 ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt
    21541 gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt
    21601 gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta
    21661 ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct
    21721 caattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc
    21781 gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact
    21841 gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagg tggggattaa
    21901 ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt tggttgcgac tgcggcagca
    21961 aaacagtctg gtgacagaaa agattctgtt tatgcttgaa ggtgccatta tcggcattca
    22021 tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca gaatgggctg actctcttac
    22081 gccgaaacag gagtttcctg cggagcttga gtaatctggc gttgcagacg accaatctct
    22141 ggcagtaatg caatcaataa cccaacttgt tgtacgacca gaggttcttt gctatctgcc
    22201 cgtggttcaa gttgctgcat ccgctgtttc aggccagcta atgcctgatt gacgcgttct
    22261 tcatcagcag gttgatgatg taacgcgtca tcaacatagc aaactgcgtc atcaagaaac
    22321 gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga gatatagctg
    22381 gtaaacgtat ggttaaggca cagcaagcga aacgcggctt cgcgaatttg cggggtaacg
    22441 ttcggttcgc tggacatatt tgataccacc gacgccagtt cagcatcacg gttgtgtgca
    22501 tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta ttgctccagt
    22561 atggcatcga gatagcgaca gttggcctct gtggcgcgtt cgagcattcg cggcagattg
    22621 cgaaacttcc agtcaggcca gatgtagctc actgccgccc acgcaatggc acaaccaatc
    22681 agcgtatcga ttacgcgagg taacgctact tcaaaacctt cacccagtaa gttaaaacac
    22741 agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt acggaaggca
    22801 aaaaagagca cgccggtaat aaccagcagc accagctgcc cttccagtga tggcacaaac
    22861 cacagcacag gaatgccaat cgcgatacct accagcgtac caataatcct taacttcagg
    22921 cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag caagatccaa
    22981 tacccgtgat gcattccggt tatttgaatg atggcgtagc cgaagcacaa caccagcgac
    23041 attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct aagacgcagc
    23101 cagatatcac tcaatccgtg cgggctgtca tcagcgagct cattttcgtc attattacgg
    23161 ggtagtgcct gggcctgttc tgattcaatt gttgccagtt gggcatcaat ggcgcgtaaa
    23221 ttgttcagca aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt atcgcgtatc
    23281 cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc atgttgataa
    23341 gactgacgca acaaaataca gcgtgacagt tgctggcacg cctggccctg catcgacatc
    23401 agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa tgtttgatac
    23461 tgaatatgag aagagctggc acgctcgtga atatcctgtg cgacaaagta ataatgcagc
    23521 gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag cgagagtttc
    23581 gtctgattca atgtcgccat cagctgaccg ttggcgagag ccaaatcgta cagcggtgcc
    23641 tggctttcat cttcaatatc aggatcaaac atgcgcgact tgagctcaag ataacgcgcc
    23701 agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa cagcagatga
    23761 ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat cggctgctga
    23821 taccagtgct catacagtga tgttcccaac atagtgtaaa tggcgatcag caatgcaccg
    23881 aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc gctggtagag
    23941 agcgttaagc caatcgcaaa tagccagggc cagggaaaca gcaattctac tgaggccgag
    24001 gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc cagtcggtca
    24061 tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt cagttttaca
    24121 tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat acgcgcgtaa
    24181 tacagccagg cgctgttcca agtatagcgt ttgagcaaag gacttagcat aaaggccata
    24241 ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg cgctgtttct
    24301 accgatacca cgcgacgacc aaccggccat agtgcaatag cggcaatttt aaagttcgca
    24361 atgccgacag gaatgccaat gattgaaata cattgtgcga tgcccgttgc aatgtgcatc
    24421 aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt accgccagta
    24481 ttcagcaaca catttttgcc agccgggttc agttcatcga catgaatagc ttcattgcca
    24541 taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg taaggtaaaa
    24601 atcagcacaa tactgaccag agtcgccaac agccagccca gagtggtggc aaatccgcca
    24661 agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac tgtggttttc
    24721 agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc tgacggttac
    24781 actgataaga aataatttcg tgtggatcta cagagtcatg gaactgaaag cgacaacgct
    24841 tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg ccgggattaa
    24901 agtctccggc gatcgccacg aataccttat tcctttcaat caattactgg cgattcactg
    24961 taagcgcggt ctggtatggg gcgagctgga atttgtactg ccggatgaaa aagtggtgcg
    25021 tctgcacggc acagaatggg gcgagacgca gcgtttttac catcatcttg atgctcactg
    25081 gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc agcaactgga
    25141 tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa cctctggcgt
    25201 tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccggttaacc gactggaaga
    25261 attcgataac tgccgtgagg cgtggcgtaa atgtcaggct tggttgaaag atattgaaag
    25321 cgctcggttg cagcataacc aggcgtatac cgaagccatg cttaccgagt atgcggattt
    25381 tttccgccag gtcgagtctt caccgctgaa tccggcgcag gcccgggcag tcgttaatgg
    25441 cgagcattct ctgttagtgc tggccggtgc aggaagcgga aaaacgtcgg tgctggtggc
    25501 ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct gagcaaattt tattgctggc
    25561 gtttggtcgc aaagccgctg aagagatgga cgagcggatt cgcgaacggc tacataccga
    25621 agacattacc gcacgcacgt tttatgcgct ggcgctgcat attattcagc agggcagcaa
    25681 aaaagttccg atagtcagca aactggaaaa tgataccgct gcccgtcatg aactctttat
    25741 tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct ggcggcaatg
    25801 gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg atgaaaaatt
    25861 acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt ctgatgcgga tgcacggtgg
    25921 tgcacaggca gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt tcagtaaacg
    25981 tatcaagttg atggccccgt tattaaaagc ctggaaaggt gcgctgaagg cagaaaacgc
    26041 tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt ctggagaaag gccgctttat
    26101 cagcccgtgg aagcatattc tggttgatga atttcaggat atctcgccgc agcgcgcagc
    26161 gttgttagcg gcgttacgca agcaaaacag tcagacgacg ttgttcgccg ttggtgatga
    26221 ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg ctttccatga
    26281 aaactttggt gaaggcgatc gctgtgattt agacacgact taccgtttta acagtcgtat
    26341 cggtgaggtg gcaaaccggt ttattcagca gaacccaggc cagctgaaaa agccgctaaa
    26401 cagcttaacc aatggagaca aaaaagctgt cacgttattg gatgagagtc aacttgacgc
    26461 tttgctggat aagctctctg gttatgccaa accggaagag cgcattctga tcctggcgcg
    26521 ttaccatcac atgaggcctg ccagcctgga aaaagcggca acacgctggc cgaagttgca
    26581 aatcgacttt atgaccattc atgccagcaa agggcaacag gcggattacg tcatcatcgt
    26641 tggcttgcag gaggggagtg atggttttcc ggctgcggcg cgggagtcga ttatggaaga
    26701 ggcgctactg ccaccggttg aggatttccc ggacgctgaa gaacggcgtt taatgtacgt
    26761 ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt aacaaagaga atccctctcc
    26821 ctttgtggaa atactgaaaa atctggatgt gccggtggcg agaaaaccgt aagaaacagg
    26881 tggcgtttgc cacctgtgca atattacttc agacggtcag cgagataacg ctgataatcg
    26941 gggataagaa tatcgaccgc gtcgttgaaa tgcggcgact ggataatgaa gtctgccgtt
    27001 gccacgttgg tggcgaccgg aatgttccat accgtcgcca gacgcagcaa ggctttcacg
    27061 tcaggatcgt gcggcacggc atttagtgga tcccagaaga aaatcaatac atcaattttc
    27121 ccttctgaga tcaatgcgcc aacctgctgg tcacccccca ttgggccact caacatcgcg
    27181 ttgacgttca tgccggtcgc gcgggaaatc aagttaccgg tagtgcctgt tgcatacagt
    27241 acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc tcatcagcat ttgtttgcag
    27301 tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt cgtcagttcc
    27361 ataatgtaca tccgtagtta actttcctac agattactgt aagcacttat cgctgcaaga
    27421 taaagaccga aaaagcctgc gcacaggcac aaaaatctca ggaagatggt tgtttttccg
    27481 cccattgcag gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa tacttcagcc
    27541 gttgttctgt gagcacctga gactgcggtg gaatagcatc cagctcggaa acacccgaaa
    27601 gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg cgtttcgcgg
    27661 atttgttata gcgttcaacc tcggcctcat aatcaatcgt ttttgccgtt gaggtaatgg
    27721 cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc atccaacaat tcaaggcgcg
    27781 gcgcggcatc aaaatggtta gcttcccgct cattctccag gcgaggcagg cgaaaattga
    27841 cctggttgat gagctgggta ttaaaactga ccaccagcgg tggggagata tacagccgtt
    27901 cttcactatt ggaaagatga atcgtcttct caacgcgaaa cactaactga tgcggtccat
    27961 tgtccagttc aatgctgtcc gcaccgcgca gcagagagct ggaaaccttt ttgccgtcga
    28021 gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg gataccggca
    28081 aacataatgc aatcaaggtg gtcacgatgc cggttttcat aggtagctcc tgtcaaaaga
    28141 ccgcccgaat aataatgtgt caaaattttt ctttaatcat acttactaac atatagacat
    28201 atttcccgct tttgctctca ttcattcgta ttagctgcat ggttggcatg tcggctttgt
    28261 cgtacacttt gttaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggcattt
    28321 taacgtccac tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgccccagct
    28381 atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag
    28441 ttgccggcaa aacgctgctg ggccagcagg ggtatggcac gctggcagac gttccggaaa
    28501 aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgtg gcacaggaag
    28561 ctattgccat tggcgcgaaa accctctgga tgcaactggg ggtgattaat gagcaagcgg
    28621 cagtactggc acgggatgcc gggttaaatg tggtgatgga tcgctgcccg gctattgaga
    28681 tccctcgcct gggtctggcc aaataaaaaa tccccggaag gcaaaaacct tccggggatt
    28741 tgttcaggga ttagttacgc agacgcgggg cctggagttg tttgcggatg gtctgcgcca
    28801 gctcatccat ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt
    28861 cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg tgataccacg
    28921 gagcggcgcg aagctcgtca ttcaccgcca gctcatcagg cgacggttca gagagcgaat
    28981 aaaccgggtc gatatccacg accactccga gataacctaa cagggaatgg cggacctgct
    29041 ggccgatacc gaatttgctg gcaatcatag tcacctcccg ggaaatcttt acctttgata
    29101 taagggtaaa attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata
    29161 gcccttccgg gtaggtagcg atcaccggat gatcggctgc ctgacggaac tgctctataa
    29221 attgtacatc acgaccggca tcaattgcgg catccgcgat gattttctga aataaatcgc
    29281 tggtcatcag accggaacag gagaaagtca ggagaatacc gccttcattc agcaactgaa
    29341 tcgccagcat gttgatgtct ttataaccac gacacgcgcc catcaactgg cttttattct
    29401 caacaaactt cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga tcgcgataag
    29461 tacgcagcaa tttaaagaca tcatcacgga caaactcagc cttgctcaga tccagtttgt
    29521 tcagctcaac gttctgccgt gcaatatcca gcgcttcctg ggaggtatca acgctgacaa
    29581 cctggctgca accgcccatc agtgccgata cggcgaaacc accggtatag gagaaacagt
    29641 tcagcacacg tttattttca acgtagcggc gggtagccag gcggctgtct cgctggtcca
    29701 gatagtagcc cgttttgtgt ccgtgctgaa tatccaccag cagcttcatg ccgtgttctt
    29761 caatcggcag cagggcaggc ggcaactcgc cggtgacgag gccctgggtc agctccatcc
    29821 cttctttttt acgtaccgca acatcgctgc gatcgtaaat cgcacattcc gggtacagcg
    29881 tttgcagggc actaattaat gccgcgcgct gatattctgc gccagcactc agcagttgca
    29941 gcaccagaaa attaccgaaa cgatcgatag taatacccgg caggccatca gattctccgg
    30001 cgattaaacg atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg
    30061 cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc
    30121 agacgcgcgc ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct
    30181 gatgatcaac aatatcgatg gtttcaccga ggctggcttt accttccatg cgggcaacgg
    30241 ccccggaaaa gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca
    30301 ctaaacgtac actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg
    30361 gagttttcca tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag
    30421 tctgcataat tgcctggatt tacgggcgag ttcagggcgt aggatttcgc tacaccacac
    30481 agtacgaagc gaaaagactg ggcttaaccg ggtacgccaa aaatcttgac gatggcagcg
    30541 tggaagtggt tgcctgcggt gaagaagggc aggtagaaaa attaatgcag tggttaaaga
    30601 gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat catccctcgg
    30661 gggaattaac cgattttcgt atccgttaaa tacattttac cggttttggc aggccagcaa
    30721 ttttggtggc ttgcttagcc gggcctttcg ggaacagtcg gtataaatag cggctgttac
    30781 ctttttcttc accaaattta ttcgccatcg cttttaccag catacgaatc gccggagacg
    30841 tattaaattc caggtagaag tcacgtacaa aacgcaccac ttcccagtgt tctggcgaca
    30901 gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg ttcactccac tggctgcttt
    30961 ctttgagata gccttcggta tccatttcta tctctttacc ttcgaagatc agcataatta
    31021 ttactacctt aatcagactg gcggcagtgt aacaaaaaac aaagccccgc ataaagcgag
    31081 gctatgaaag tgttagcggg tgagattaat cgcggctggc gaagcccaga atgctcagca
    31141 ggctgacgaa gatgttgtac agcgaaacat acaggctaac cgtggcacga atatagttcg
    31201 tttcaccgcc atgaatgatg ttgctggttt caaacaagat agcgccagag gagatcagaa
    31261 tgaagaccgc gctgatcgcc agatgcagag caggcagttg caggaagata ttagcaacca
    31321 taccaatcag taccaccaca atacccgcca tcagcatacc gccgaggaac gacatatctt
    31381 tgcgggtggt cagcacatat gcagagcagc agaagaacac taacgccgtt ccgcccagtg
    31441 ccatagcgat tacgtcaccc attccggcag acagataggt gttcagaatt ggtccgagga
    31501 tataacccag aaaaccggta aaggcgaatg cggagataat cccggtcggc ttattcgccg
    31561 ttttataggt caggaacatc aaaccataca tacccaccag cgtcagaatc agacccggag
    31621 atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc agcgtcaggc
    31681 tcagcagaaa ataggtatta cgcagcacct tatgggtgct aagcagtgat gtacggtcat
    31741 gtgaagaact aacaatacga tccattgagt cactctctat gacagatgta attaattaag
    31801 cagcataatg ataatgcgta agggcaccca gaagttttac ccatctttac gtatttgatc
    31861 tggaacaggt ttaacagcgg attatcaggt cattaagcaa atataacgcc ctgagaattc
    31921 cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc cttatcttat ttggcggaag
    31981 cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc ggtgccttca
    32041 accgctcggc cacacttccg gaatgacgcg cactataaac atcccgatgc ggcgtgtaaa
    32101 cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc gtgaaataac
    32161 agcagattga tcatttcatc accatgaatt ccttctcttt tactcgttta gcaaccggct
    32221 aaacatcccc atcgcccggc taaagaaaaa taggtccatt tttatcgcta aaagataaat
    32281 ccacacagtt tgtgttgttt tgtgcaaaag tttcactacg ctttattaac aatactttct
    32341 ggcgacgtgt gccagtgcag aaggatgagc tttcgttttc agcatctcac gtgaagcgat
    32401 ggtttgcctt gctacaggga cgtcgcttgc cgaccataag cgcccggtgt cctgccggtg
    32461 ttgctaggag aagagacgtg cgatatgaat aacgaggaaa cattttacca ggccatgcgg
    32521 cgtcagggcg ttacccggcg cagctttctc aaatattgta gtctggctgc cacgtcgctg
    32581 ggattaggcg cgggaatggc accaaagatt gcctgggcgc tggagaacaa accgcgcatt
    32641 ccggtggtat ggatccacgg tctggaatgt acctgctgta ccgaatcttt tatccgctcc
    32701 gctcacccac tggcgaagga cgtcatcctt tccctgattt ccctctatta cgacgatacc
    32761 ctgatggctg ccgccggaac ccaggcggaa gaagtctttg aagacatcat cacgaaatat
    32821 aatggcaaat atatcctcgc agtagaaggt aatccgccgc tgggcgagca ggggatgttc
    32881 tgtatcagca gcggtcgacc gtttattgag aaactcaaac gtgccgctgc cggagccagc
    32941 gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg tgcaggccgc gcgacctaat
    33001 ccgacacagg caacgcctat cgacaaagtc atcaccgaca aacccattat caaagtacct
    33061 agctgcccgc cgatcccgga tgtgatgagc gccatcatta cttacatggt gacctttgat
    33121 cgcttgccag atgtcgacag aatgggtcgt ccactgatgt tttatggtca gcgtatccac
    33181 gataaatgct atcgccgcgc tcacttcgac gccggagagt tcgtccagag ttgggatgat
    33241 gacgctgccc gcaaaggtta ttgcctgtac aaaatgggct gcaaagggcc taccacctat
    33301 aacgcctgtt cctccacacg ctggaatgat ggcgtttctt tcccaatcca gtctggtcac
    33361 ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg gttcgttcta cagccgcgtg
    33421 gtcgatattc cgcaaatggg tactcattcc accgccgata ccgtcggttt aaccgcgctt
    33481 ggcgtggtgg cagcagctgt tggtgtgcac gcagttgcca gcgccgttga ccaacgcaga
    33541 cgtcataacc agcaacctac agaaaccgaa catcagccag gcaatgagga taaacaggca
    33601 tgagcactca gtacgaaact cagggataca ccatcaataa tgccggacgc cgcctggtgg
    33661 tcgacccgat tacgcgcatc gaaggccaca tgcgctgcga agtgaatatt aacgatcaga
    33721 atgtgatcac caatgccgtc tcctgcggca ccatgtttcg cgggctggag atcatcctgc
    33781 aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg tatctgcggc gtctgtactg
    33841 gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc catcggtatt aaagtgccgg
    33901 acaacgccaa tatcatccgc aacattatgc tgacaacgct ctggtgccac gatcatctgg
    33961 tgcacttcta tcagcttgcc gggatggact ggatcgacgt gttagatgcg ctgaaagccg
    34021 acccgcgaaa aacctccgaa ctggcgcaaa gtctctcctc ttggccgaaa tcatccccta
    34081 gctatttctt cgacgtacaa aatcgcctga agaaattcgt tgaaggcggg cagttgggga
    34141 tcttccgcaa tggcaactgg gggcacccgc agtacaaatt gccgccagaa gccaacctga
    34201 tgggctttgc ccactatctc gaagctctcg atttccaacg tgaaattgtc aaaattcacg
    34261 cggtctttgg cggtaaaaac ccgcatccaa actggattgt cggcgggatg ccttgcgcca
    34321 ttaacattga cgaaagcggc gcggtcgggg cggtcaatat ggaacgcctg aacctggtgc
    34381 agtcgattat cacccgcacg gcagacttca ttaacaacgt gatgatcccc gacgccttag
    34441 ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac gggtctttct gataaatgtg
    34501 tcctcagcta cggcgcattc ccggatattg ccaacgactt tggtgagaaa agtctgctga
    34561 tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt gctgccagtg gatttggttg
    34621 atccgcagca ggtgcaggag tttgtcgatc acgcctggta tcgttatccc aacgatcagg
    34681 tcgggcgtca tccattcgat ggcattaccg acccgtggta caaccccggc gatgtcaaag
    34741 gcagcgatac caacattcag cagctgaatg aacaggaacg ctactcgtgg atcaaagcgc
    34801 cacgctggcg cggtaacgcg atggaagtgg ggccgctggc acgcacgtta atcgcttatc
    34861 acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat gatgtcggcg ttgaacctgc
    34921 cgctttccgg tatccagtca acgttgggcc gcaggtaagt tgcagtattt cttcgacaag
    34981 ctgatgacca acctgaaaaa tggcaatctc gccactgctt ccactgaaaa atgggaacct
    35041 gcaacctggc cgacagagtg ccgtggcgtc ggctttaccg aagcgccgcg cggagcgtta
    35101 ggccactggg ccgccattcg cgatggcaag attgatctct accagtgcgt ggtgccgacc
    35161 acctggaacg ccagcccgcg cgatcccaaa gggcagattg gcgcttatga agcggcgctg
    35221 atgaacacca aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc
    35281 tttgatccgt gcctcgcctg ttcaactcat gtgctgggcg acgacggtag cgagctgatc
    35341 tccgtgcagg tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt
    35401 gtcagccact atgtctttga agcaccagtt cgtatctggc actggctgac ggtgttatgc
    35461 atggcggtgt tgatggtcac cggttacttt atcggcaagc cgctaccttc cgtcagtggc
    35521 gaggcgacgt atctgttcta tatgggttac atcaggttaa ttcacttcag cgccgggatg
    35581 atttttaccg tggtcttgct gatgcggatc tactgggctt ttgttggcaa tcgttactcc
    35641 cgcgagctgt ttatcgtgcc ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa
    35701 atccgctggt atctgtttct ggcaaaacgt ccgagtgctg atataggcca taaccccatc
    35761 gcccaggcgg cgatgttcgg ctatttcctg atgtcggtct ttatgatcat caccggtttt
    35821 gcgctgtaca gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa
    35881 tttttctact ggacgggtgg caactcaatg gacattcaca gctggcatcg gctggggatg
    35941 tggctgattg gggcgtttgt gatcggtcat gtctacatgg cgctgcgtga agacatcatg
    36001 tccgacgaca cggtgatctc caccatggtc aacggctacc gtagccacaa atttggcaaa
    36061 ataagtaaca aggagcgttc atgagcgagc aacgcgtggt ggtcatgggg ctgggcaacc
    36121 tgctgtgggc cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat gcccattacc
    36181 actggcccga gtatgtggag attgtcgatg gcggtactca gggactgaac ttgctggggt
    36241 atgtcgaaag cgccagccat ctgttgattc tcgatgccat tgactacggg ctggaacctg
    36301 gaaggctgcg aacctatgcc ggagaacgca ttccggccta tctcagcgcg aagaaaatga
    36361 gcctgcatca gaacagtttc tccgaagtgc tggcgctggc agatatccgc ggacatctgc
    36421 cagcacatat tgccctcgtc gggctgcaac ccgcgatgct cgacgactac ggcggcagcc
    36481 tgagcgaact ggcacgggag caactgcccg ctgcggaaca atcggcgctg gcgcagttgg
    36541 ctgcgtgggg aagtgtgccg caaccggcta atgaatcgcg ttgtctcaat tatgactgtc
    36601 tgtcgatgga aaattacgaa ggcgttcgct tgcgccagta ccggatgaca caggaggagc
    36661 agggatgagc aacgacacgc catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg
    36721 gacgccggtc agtgaatccc gtcttgacga ctggcttacg caagcgccag acggcgtggt
    36781 gttattaagc agtgacccga aacgcacgcc agaggtcagc gataatccgg taatgattgg
    36841 cgaattactg cacgagtttc ccgactatac atggcaggtg gcgattgctg accttgagca
    36901 gagcgaagcc atcggcgatc gctttggcgc ctttcgcttt cctgccactt tagtgtttac
    36961 cggcggaaac tatcgcggcg tgctgaatgg tattcacccg tgggcggaac tgataaacct
    37021 gatgcgcggg cttgtcgaac cgcagcagga gcgtgcctca tgagcgaaac ttttttccat
    37081 ctgctggggc caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc
    37141 acctgtcagg tgaatgatga accgagtatg gcggccttgg agcaatgtgc tcacagcccg
    37201 caggtgattg cgctgttaaa cgagttacaa catcaactaa gcgaacgcca gccgccgttg
    37261 ggcgaggtgc tggcagttga tctgttaaat ctcaacgccg acgatcgtca ctttatcaat
    37321 acgcttctcg gggaagggga agtgtcagtg cgcattcagc aggctgacga cagtaaaagt
    37381 gaaatacagg aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg cggcgaaaag
    37441 ttgctggagg acaaactgga ggctggctgc gcgccgctgg cgttgtggca ggcggcaacg
    37501 caaaatctct tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc
    37561 ctaccgttgg cgcatgagtt actggcacat gtacgtaacc ccgacgcgca gccgcacagc
    37621 attaatctga cgcaattacc catcagcgag gctgatcggc tttttctctc acgtctctgt
    37681 ggtccgggaa atattcagat tcgtaccatt ggctatggcg agagctatat caactccacg
    37741 gggttacgcc atgtctggca tttacgctgt acggacacct taaaaggccc gttactggaa
    37801 agttatgaaa tctgcccaat accggaagtg gtgctggcag cgccagaaga tttggtcgac
    37861 tctgcgcagc ggcttagcga ggtatgtcag tggctggcgg aagctgcacc gacgtaaaaa
    37921 gacggtaagt atcgctttca gtcttatgaa tatcgcaatc ggcgaatacc tctggtcgta
    37981 gagtttcagg ataaagaggg agatctaaca ttatcgggtt atttttctct cttcgcctac
    38041 aggagtgcgc atgtgggatg tcattgattt atcgcgctgg cagtttgctc tgaccgcgct
    38101 gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg ctatcatgga
    38161 aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa
    38221 gctcttcggt atcaattttg ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt
    38281 tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg caccactggc
    38341 gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg
    38401 ctggcaacgg ctgaataaat accagcacct gctggtgacg tggctggtgg cgttcggttc
    38461 aaatctctct gcattgtgga tattgaatgc caacggttgg atgcaatacc cgaccggtgc
    38521 gcattttgat atcgacaccc tgcgtatgga gatgaccagc ttcagcgagc tggtctttaa
    38581 cccggtcagc caggtgaaat ttgtgcacac cgtcatggcg ggctacgtga ccggggccat
    38641 gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgtcgcatt
    38701 acgctcgttt gccatcggtt ccgtcttcgg tactctggcg attatcggta ccctgcaact
    38761 cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga
    38821 aggggagtgg caaacggaac ctgctcctgc accgttccat gtggttgcct ggccggaaca
    38881 ggatcaagag cgtaacgcct ttgccatcaa aattcccgcg ctgctaggga tcctcgccac
    38941 tcactcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa cctacccacg
    39001 cttgcaacgc ggacgtatgg cctggctgtt aatgcaggaa atatctcaag gcaatcgtga
    39061 gccgcatgtg ttgcaggcat tccgggagct ggaaggcgac cttggttacg gcatgttgct
    39121 ctcccgctat gcgccggata tgaatcatgt cacagccgca cagtaccagg cggcgatgcg
    39181 tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg
    39241 ttccctgctg ctactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga
    39301 ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccgttgccgt ggattgcgat
    39361 tgaagcaggg tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt
    39421 accgacatac tccgcgcact ccgctttaac cacaggacaa ctggctttct cactgatcat
    39481 gatcgtaggt ctttacaccc tgttcttaat cgccgaagtc tacctgatgc agaaatatgc
    39541 ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag
    39601 aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc
    39661 ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgttt gctgccgctg
    39721 gtggcgcgta atgatgatga acgccggata gtgattaata gcgttggtgc gcactgggaa
    39781 ggcaatcagg tctggttgat cctcgcaggt ggggcattat ttgccgcctg gcccagagtg
    39841 tatgcagcgg cgttttccgg cttttatgtg gcgatgatcc tggtgctgtg ctcgctgttc
    39901 ttccgtccgc tggcctttga ttatcgcgga aaaattgccg atgcacgctg gcgtaaaatg
    39961 tgggatgccg gtctggtcat cggcagtctg gtgccgccgg tagtcttcgg tatcgccttc
    40021 ggcaacttgt tgctcggcgt gccgtttgcc ttcacaccgc aattacgcgt ggagtatctc
    40081 ggcagcttct ggcaactgct gacgccattc cctttattgt gtggtttgct cagccttggg
    40141 atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg
    40201 cgttcacagc tggcgactaa acgcgctgca ctgttggtga tgctgtgctt tttgctggca
    40261 ggttactggc tgtgggtcgg tattgatggc tttgtactgc tcgcccagga tgctaacggt
    40321 ccttccaatc cgttaatgaa actggtggca gtgctacctg gtgcctggat gaataatttc
    40381 gtcgaatcgc ccgttttgtg gatcttcccg ctgctggggt tcttctgccc attgctgacg
    40441 gtgatggcga tttatcgtgg tcgcccgggt tggggatttt tgatggcatc attgatgcaa
    40501 tttggcgtga ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc gtcaagtgtg
    40561 agtccaatct ccagcctgac gttgtgggac agcacttcca gtcagctgac gctgagcatt
    40621 atgttggtaa tcgtgctgat atttttgccc attgtgttgc tctacactct ctggagctac
    40681 tacaaaatgt ggggacgcat gacaacagaa actctccgcc gtaacgaaaa cgagttgtac
    40741 taaggagcag gaacatgtgg tatttacttt ggttcgtcgg cattttgttg atgtgttcgc
    40801 tctccaccct tgtgttggta tggctggacc tgcgtctgaa aagttaacga acgtaggcct
    40861 gatgcggcgc attagcatcg catcaggcaa tcaataatgt tagatatgaa aagcggaaac
    40921 atatcgatga aagcgatctt aatcccattt ttatctcttc tgattccgtt aaccccgcaa
    40981 tctgcattcg ctcagagtga gccggagctg aagctggaaa gtatggtgat tgtcagtcgt
    41041 catggtgtgc gtgctccaac caaggccacg caactgatgc aggatgtcac cccagacgca
    41101 tggccaactt ggccggtaaa actgggttgg ctgacaccgc gcggtggtga gctaatcgcc
    41161 tatctcggac attaccaacg ccagcgtctg gtagccgacg gattgctggc gaaaaagggc
    41221 tgcccgcagt ctgctcaggt cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca
    41281 ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa taaccgtaca tacccaggca
    41341 gatacgtcca gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg ccaactggat
    41401 aacgcgaacg tgactgacgc gatcctttgc agggcaggag ggtcaattgc tgactttacc
    41461 gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc ttaatttccc gcaatcaaac
    41521 ttgtgcctta accgtgagaa acaggacgaa agctgttcat taacgcaggc attaccatcg
    41581 gaactcaagg tgagcgccga caatgtctca ttaaccggtg cggtaagcct cgcatcaatg
    41641 ctgacggaga tatttctcct gcaacaggca caaggaatgc cggagccggg gtggggaagg
    41701 atcaccgatt cacaccagtg gaacaccttg ctaagtttgc ataacgcgca attttattta
    41761 ctacaacgca cgccggaggt tgcccgcagt cgcgccaccc cgttattgga tttgatcatg
    41821 gcagcgttga cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt acccacttca
    41881 gtgctgttta ttgccggaca cgatactaat ctggcaaatc tcggcggcgc actggagctc
    41941 aactggacgc ttccaggtca gccggataac acgccgccag gtggtgaact ggtgtttgaa
    42001 cgctggcgtc ggctaagcga taacagccag tggattcagg tttcgctggt cttccagact
    42061 ttacagcaga tgcgtgataa aacgccgctg tcattaaata cgccgcccgg agaggtgaaa
    42121 ctgaccctgg caggatgtga agagcgaaat gcgcagggca tgtgttcgtt ggcaggtttt
    42181 acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt tgtaatgcat aaaaaagagc
    42241 attcagttac ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta atgcgtagac
    42301 cggaaaaggc gttcacgccg catccggtca ctttcagttt tactctttct cggagtaact
    42361 ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt
    42421 gaggattgcg cctttaatat taacgccagc ctgttccaga cgctgcattg acaagctcac
    42481 ctctttggcg gtgttcaagc caaaacgcgc aaccagcagg ctggtgccga cagagcgtcc
    42541 cacgaccgca gcatcgctca ccgccagc
//
LOCUS       DADMFL010000020        41582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.020, whole genome shotgun sequence.
ACCESSION   DADMFL010000020 DADMFL010000000
VERSION     DADMFL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.020"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(7..300)
                     /gene="ybgE"
                     /locus_tag="JNZ27_001117"
     CDS             complement(7..300)
                     /gene="ybgE"
                     /locus_tag="JNZ27_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY8663531.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(300..413)
                     /gene="cydX"
                     /locus_tag="JNZ27_001118"
     CDS             complement(300..413)
                     /gene="cydX"
                     /locus_tag="JNZ27_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY8663532.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(428..1567)
                     /gene="cydB"
                     /locus_tag="JNZ27_001119"
     CDS             complement(428..1567)
                     /gene="cydB"
                     /locus_tag="JNZ27_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8663533.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1583..3151)
                     /gene="cydA"
                     /locus_tag="JNZ27_001120"
     CDS             complement(1583..3151)
                     /gene="cydA"
                     /locus_tag="JNZ27_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY8663534.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(4000..6633)
                     /gene="mngB"
                     /locus_tag="JNZ27_001121"
     CDS             complement(4000..6633)
                     /gene="mngB"
                     /locus_tag="JNZ27_001121"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY8663535.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(6651..8627)
                     /gene="mngA"
                     /locus_tag="JNZ27_001122"
     CDS             complement(6651..8627)
                     /gene="mngA"
                     /locus_tag="JNZ27_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY8663536.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAVDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            8737..9459
                     /gene="mngR"
                     /locus_tag="JNZ27_001123"
     CDS             8737..9459
                     /gene="mngR"
                     /locus_tag="JNZ27_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY8663537.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(9563..10432)
                     /gene="sucD"
                     /locus_tag="JNZ27_001124"
     CDS             complement(9563..10432)
                     /gene="sucD"
                     /locus_tag="JNZ27_001124"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY8663538.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10432..11598)
                     /gene="sucC"
                     /locus_tag="JNZ27_001125"
     CDS             complement(10432..11598)
                     /gene="sucC"
                     /locus_tag="JNZ27_001125"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY8663539.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(11692..12909)
                     /gene="odhB"
                     /locus_tag="JNZ27_001126"
     CDS             complement(11692..12909)
                     /gene="odhB"
                     /locus_tag="JNZ27_001126"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY8663540.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVMSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(12924..15725)
                     /gene="sucA"
                     /locus_tag="JNZ27_001127"
     CDS             complement(12924..15725)
                     /gene="sucA"
                     /locus_tag="JNZ27_001127"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY8663541.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(16026..16742)
                     /locus_tag="JNZ27_001128"
     CDS             complement(16026..16742)
                     /locus_tag="JNZ27_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY8663542.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(16758..18524)
                     /gene="sdhA"
                     /locus_tag="JNZ27_001129"
     CDS             complement(16758..18524)
                     /gene="sdhA"
                     /locus_tag="JNZ27_001129"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY8663543.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(18524..18871)
                     /gene="sdhD"
                     /locus_tag="JNZ27_001130"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(18524..18871)
                     /gene="sdhD"
                     /locus_tag="JNZ27_001130"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY8663544.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(18865..19269)
                     /gene="sdhC"
                     /locus_tag="JNZ27_001131"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(18865..19269)
                     /gene="sdhC"
                     /locus_tag="JNZ27_001131"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8663545.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            19650..19757
                     /locus_tag="JNZ27_001132"
     CDS             19650..19757
                     /locus_tag="JNZ27_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663546.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            19963..21246
                     /gene="gltA"
                     /locus_tag="JNZ27_001133"
     CDS             19963..21246
                     /gene="gltA"
                     /locus_tag="JNZ27_001133"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY8663547.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            21637..22203
                     /locus_tag="JNZ27_001134"
     CDS             21637..22203
                     /locus_tag="JNZ27_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8663548.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            22254..24713
                     /locus_tag="JNZ27_001135"
     CDS             22254..24713
                     /locus_tag="JNZ27_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8663549.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            24728..25459
                     /locus_tag="JNZ27_001136"
     CDS             24728..25459
                     /locus_tag="JNZ27_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8663550.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTTYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            25456..26517
                     /locus_tag="JNZ27_001137"
     CDS             25456..26517
                     /locus_tag="JNZ27_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663551.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            26624..27715
                     /locus_tag="JNZ27_001138"
     CDS             26624..27715
                     /locus_tag="JNZ27_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY8663552.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMILGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGFMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(27712..28503)
                     /gene="nei"
                     /locus_tag="JNZ27_001139"
     CDS             complement(27712..28503)
                     /gene="nei"
                     /locus_tag="JNZ27_001139"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY8663553.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(28539..29273)
                     /gene="pxpA"
                     /locus_tag="JNZ27_001140"
     CDS             complement(28539..29273)
                     /gene="pxpA"
                     /locus_tag="JNZ27_001140"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY8663554.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPVLVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(29263..30195)
                     /gene="pxpC"
                     /locus_tag="JNZ27_001141"
     CDS             complement(29263..30195)
                     /gene="pxpC"
                     /locus_tag="JNZ27_001141"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY8663555.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(30189..30845)
                     /gene="pxpB"
                     /locus_tag="JNZ27_001142"
     CDS             complement(30189..30845)
                     /gene="pxpB"
                     /locus_tag="JNZ27_001142"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY8663556.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(30868..31611)
                     /gene="ybgI"
                     /locus_tag="JNZ27_001143"
     CDS             complement(30868..31611)
                     /gene="ybgI"
                     /locus_tag="JNZ27_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY8663557.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            31882..33363
                     /gene="dtpD"
                     /locus_tag="JNZ27_001144"
     CDS             31882..33363
                     /gene="dtpD"
                     /locus_tag="JNZ27_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY8663558.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYKPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(33513..34931)
                     /gene="phrB"
                     /locus_tag="JNZ27_001145"
     CDS             complement(33513..34931)
                     /gene="phrB"
                     /locus_tag="JNZ27_001145"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY8663559.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(34928..35437)
                     /locus_tag="JNZ27_001146"
     CDS             complement(34928..35437)
                     /locus_tag="JNZ27_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY8663560.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            35566..35883
                     /locus_tag="JNZ27_001147"
     CDS             35566..35883
                     /locus_tag="JNZ27_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY8663561.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(35938..36318)
                     /locus_tag="JNZ27_001148"
     CDS             complement(35938..36318)
                     /locus_tag="JNZ27_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663562.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(36321..40985)
                     /locus_tag="JNZ27_001149"
     CDS             complement(36321..40985)
                     /locus_tag="JNZ27_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY8663563.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYRFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPW
                     ET"
     gene            complement(41004..41225)
                     /locus_tag="JNZ27_001150"
     CDS             complement(41004..41225)
                     /locus_tag="JNZ27_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY8663564.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
BASE COUNT         9955 a        11423 c        10133 g        10071 t
ORIGIN      
        1 tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg caagcaatgg
       61 gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt gaatcacacc
      121 agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac tggtcttcgc
      181 ggcaaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca tcacgaagga
      241 aagcgcccgc agggggcgct tgtccattac cgcatacaag gtcgcgataa tcttactcat
      301 cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt caagcgccag cgcggtgatt
      361 accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca catttttagc
      421 tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg atacgaccga
      481 acattttcca gtaacaccag gcggtgtaga gcagaatgat cggtaccaga accaccgcaa
      541 cccaggtcat gacgttaagc gtcagctggc tggaagttgc atcccacatt gtcagacttg
      601 cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg gctgtcagga
      661 tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta tccatacgtg
      721 cagtcaggat ggtcagcagc ggcagaacca cacccagtgc cggaatagcc cacagaattg
      781 gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct ttattcagtg
      841 ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg ataccgtaca
      901 tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagcgcagcc acctgagccg
      961 ttgcacgggt acgcaggtgc agttcgcccc cggtacgcat ttgcagatag gttgcgccct
     1021 gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg ttaagcaact
     1081 ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac ggtacgccct
     1141 gcaacaggtt accgaacgct acaccaatta ccagcggcgg aacgaagcta ccaatgaaga
     1201 tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg tagtcaaaac
     1261 cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag aagccggaga
     1321 acgcagcggc atagaccatc ggccaggcag caaagagtgc gccgcccgcg gtgatcagcc
     1381 aaacctggtt accgtcccag tgtggtgcaa tggagttaat cataattcga cgctcggtgt
     1441 cgttacgacc gaggaaacgg gtgagcatgc ccacccccat gtcgaaaccg tcagtgactg
     1501 caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat acttcataat
     1561 cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa gactgctcaa
     1621 agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg aacattaaga
     1681 acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt gagaagatga
     1741 gatcgcctgc ggtcagtgac gaattcgcca cagctgtcgg cagcacttca ccgatagccc
     1801 acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc cacggcagcg
     1861 gaataccgta cagcgcggcg cgcagaagcc attttttctc gccaatgcgg ttgcggatga
     1921 cactccagaa agagagcgcg atgattgcca gcagcaggaa gccacacgcc accatgatac
     1981 ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc tttggttgcc tgttgaatct
     2041 gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga ccgtaaccga
     2101 ggtctttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa ccagaacgca
     2161 gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca tgctgcacca
     2221 tcagctcttt caggccgata accggggtat ccacggaacg cgttgcaatg atgcccagtg
     2281 cgtaagggat ctgaatcgca aatttgttcg tctcctcttc ctgatcagga atgccgaaca
     2341 aagtaaaggc agcaggcgca ggttgcgttt cccactcggc ttcaatagca gccagtttgg
     2401 ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata acagacagaa
     2461 cagcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag gcgaagtcac
     2521 gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca gtcacataac
     2581 cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc agctcggaga
     2641 agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt gggttttgca
     2701 tccagccgtt cgcaatcaga atccacagtg cggacaggtt tgaaccgagc gccaccagcc
     2761 aggtgacaca catatgctga actttaccca gacgatccca accgaagaag aacagaccta
     2821 caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc gcaccgaaga
     2881 tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac tccatggtca
     2941 gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag aacttggtca
     3001 tatctttata aatctgtttg ccggagagga cgtagaccgt ttccataatg gccaggagga
     3061 acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc aaggcaaact
     3121 gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg catgaagact
     3181 ccgagagtga accccgttag aaagggtcac acgcatgccc caatagaaat ttgtttgaac
     3241 actttcatca taccatgtga atgcacacaa aaacggcgta aagaaaggtt gcaaacatgt
     3301 taataaaaac tcaaattgat cccacgtata tattacgccg caaaatcctt acaataaaca
     3361 ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc aatttatagc taaattgccg
     3421 cctttcagcc aatttgatcg agaacaattt atctcttttt gatgcccatt tccaagactt
     3481 atacattgat aaatatcaat ttctacaaat tatgttaatc atgtttcaac aaggtaactt
     3541 tagtgacatt tatgtttaaa atgtgtgaat tataagactg aaattagttt cggttggcaa
     3601 ttaaagataa aaagagacga agcaaaaaca gcacggttga tcttaattct ttttttggtc
     3661 actggctaat acgacgcaga gtttcaggat tattaataaa ggcataaatc tcctccccgc
     3721 cgatattaac atttaattaa cttataatta tattataatt aactcacccg gggttattat
     3781 tttgttaaaa ataaagccac atatacgtag cttgagagtg aagataaacg caaaattacc
     3841 tgatgcgcta cgcttgccag tgtctacatc atctctgcaa tatattgaat ttgcctgctt
     3901 ttgtagaccg aataaagcgt tcatgccgta tccggcatga acaaagcgcc ctttgtcaac
     3961 aatctggccg cgcatatgtg gcttcggttt gattgctatt caggcaagcc ggtaactgaa
     4021 cgtccgtgac tggccgggta aaaaaggccc cgatagattt gaaccttgat tttcctcggt
     4081 ggtaatgtgt tcatccatca tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac
     4141 agtcgcatca caggttgctg attcagccgg attaaacagc cgtaaaatca cttcttgtcg
     4201 gtcttcagct ttcttaagtg cgcttatcag gcatcccact gggggcattt tcaacaaact
     4261 ataactttcc ggcacgttga atccggcttt gttgagcttc atcgcatccc atgggatttt
     4321 gttgtaacac tgtactggag tcagccatgc tcgcgcctgc tgagctacac cagcggcggt
     4381 tggcgtaccg gtataactca ataaacttag gcgacaagaa agcagaccac gtagttgtga
     4441 gtccgggact ggcattttaa ttcccgaagg ccgcctaggc cttaaaagca gatcttcttt
     4501 ccccagtaag cccacgccgc gcagcaacgt aatggcaaag gttttcttct cttcaccgat
     4561 gacttcaaat tcacgtagcc cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa
     4621 ggctgcatag ttgagcatat tccataccgg aaccggcgct tctttccagc cttcttgctg
     4681 ccagttgttc attgcactgt cgttcacggg gcgcgttagc gaaccaaact gggtatctgc
     4741 cagaacacta tcggtgttaa aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc
     4801 ctggttatca aggttgatat ccacatcaat gcgcctgttg ttatgactaa gagtgacaac
     4861 caactcgaca cctactctgc cagtggattg tcttgcgctg cgttgttgca gattacgcgg
     4921 caccgccatt tcatagcgga taacagccct gctctgccag gcctcatgaa taatctcgca
     4981 ttgcggtttc gcattcgccg aagtgattac ccactcttct tttgcgggtg aatagtcata
     5041 ttcatcacca tcatcagagc tttcttcaat ttgcaacacc cggtcatagc gcacaccgct
     5101 atctttatct accagttgta gcgttccatc ctcattgagc gcaatttgcc agaaagcatt
     5161 ttccagtatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct gattcgcttc
     5221 gatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgta tatcaaactc
     5281 cataaaggga tcgtaattac cgtaatgaac tatttgccga tcgattaggc ctggatcgat
     5341 ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctc gtagattaaa
     5401 ctggctggcg cgcagccgca cagtggtgtt gatcacttct tcccgcggcc agggcatcag
     5461 gttaaacagg acgagtttgt cggcgtcgct ctgcggcatg ttgtcggcga ttttacgcat
     5521 atagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg cgacgatttc
     5581 gcgatgaact ttgtcactac agcagcagcc gatgctgtcg tgggcatgat tttttaagat
     5641 ctctttccat attttctcca gcaagccgtg gtggtattca aaacccaacg tccaggccag
     5701 cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt gggcaacttt
     5761 gatatccatg cgcgtggaac cgatggtgcg atgcacgcgc atatatttgc catcaataaa
     5821 ttcccctttc agggttgcca gattatctcg ctgcgcttcg atcttctcaa atacctcttc
     5881 aaagcggctc atcacaaact ttcgttgagg ataaatttcg cgcagtttct ccatcacttc
     5941 gaagatattt tgctgcaatg gcatctgatc atgcccattc ggcagcaaaa tctctttggt
     6001 tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttgcgga atccgttttc
     6061 gtccgcaggt aagtacttac cgatggcata acccaacggc atcacctgcg ccgtcacttc
     6121 gctaccgtca ctgctttgcc acaaaaactc ggttttatcc gtaccgtggc gctcagaaca
     6181 tccacgccag aacatggtgc aggtaatgcc aaatccattg tagatatgcg gcagttgccc
     6241 ggacatgcca aaggaatcgg gtaaataacc tattttcatc ggctcgccaa acgcgaggca
     6301 gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa tcgtggtatc
     6361 ggtctgggta taccaggggc cgataatcaa cttgccggct tctaccagtt tcttcacgcg
     6421 gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag
     6481 tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt ccatattatt
     6541 gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc agtgcatatg
     6601 cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt ttattgcatt
     6661 acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat caggagaatt
     6721 gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc cgccataaca
     6781 ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccagcacc gggtgtcgaa
     6841 aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag cacaaacgaa
     6901 ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc
     6961 agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa caggcgcggt
     7021 gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg
     7081 gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata agcggcttta
     7141 ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc cagcaacagc
     7201 gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat ccaggcgaca
     7261 ggttccccca ccacaaacag catcagactg cccgctccca atgtaccgag caccaggtag
     7321 agataaaaag tcaggaagcc attgaattta ctgctaagac gcaagtgatt tttcatccag
     7381 cgtatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc ggagccgatc
     7441 atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc tgccagcgaa
     7501 taggcggtat aggctgccag caccggtacc atcaaaattc cgagcagccc gccgcccagt
     7561 ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag cccgaagatt
     7621 tgcgacagta ataccgcgac cgccagcacc gtcccccccg cgacaatcaa cggtacggca
     7681 aaagagatcc cgctcaacag tgcctgtttc agctccgttt tgacgctttt caccggttgc
     7741 gtatcttgct gtacggtacg cgtctcatcg ccacgctcga gggtaagcga ttgttggatc
     7801 aacgcttctg catggcgaat cggctcggca acaggcacag aaagcgcagg aatgccgtta
     7861 aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcagt tgcactattg
     7921 agttgatccg ccgttaaacg cccttcaata ccgttagcac cttgcttttc aacgtagaca
     7981 ttcacgccga gtttgcgtcc ggctttttcc agatactccg cagccatgta ggtgtgagca
     8041 atacccgccg ggcaggccgt tacgcaaacg atagttggcg cgttggaaaa agaggcagaa
     8101 ggttgcgtgc ctcccttgtc atccagcgcc gagagcaatt catcaggcgt cgtcgccgac
     8161 tgtatacgcg cccgaatctc atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc
     8221 atgtgcgtag tacccgcttc attgggggga atcgccagca acaccactaa atcaaccgct
     8281 tccgggccat ccacgctttc ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc
     8341 gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcacc taaggcggtc
     8401 gggccaaggc tttcacggcg atacacttct ttcagaaatt gctcagtact ggaaattttc
     8461 cccagagcag caagacgttg agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc
     8521 gcattcagac acaacgcatc gcggtgggtc agagtcgtca ggttcatcat gatcctcact
     8581 tgatcgtttt tatagtcgcg ccagtgtgcc cgataaaaca ataccattgc gtaatcaatt
     8641 tcacttgatt gaaattattt acattgtatt tgtattattt acaggatgaa tttatcgggc
     8701 ataatggcta aattcgtatt aatgagatgc taaataatgg gacacaaacc cttataccga
     8761 cagattgctg atcgcattcg tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg
     8821 ttacctaccg aatcggcatt acaaacagag tttggcgtca gccgggttac ggtgcgtcag
     8881 gctttgcgcc agttagtcga gcaacagatc ctcgaaagca ttcagggcag cgggacttac
     8941 gtcaaagaag agcgggtcaa ttacgatatt tttcagttaa ccagttttga tgaaaaactg
     9001 tcggaccgtc acgtcgatac acacagtgaa gttctgatat ttgaagtgat tccggctgac
     9061 gattttcttc agcaacagct acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc
     9121 gtgcgctatc gcaagcaaaa gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg
     9181 ttcccggatc tcacctggca ggtcatggaa aattcgaaat atcactttat tgaagaagtg
     9241 aagaagatgg ttatcgatcg tagcgaacag gaaatcattc ctctgatgcc aaccgaagag
     9301 atgagccgtc tgcttaatat cagccagaca aagccgattc ttgaaaaagt atcgcgtgga
     9361 tatttagttg atggtcgggt gtttgaatat agccgcaacg cttttaatac cgatgactat
     9421 aaatttaccc tgatagcgca gcgaaaatca tcgcgataag cacaaaaaag gccatcatac
     9481 gatggccaac catgtcgaaa ccgacagccc ttcgccattc ctgtagaggg aagcggcgag
     9541 ggctatttct tattacagat atttatttca gaacagtttt cagtgcttca ccgatatccg
     9601 ccaggctgcg aacggttttc acgcctgcgg cttccagagc agcgaatttc tcatccgcag
     9661 tccctttccc accggcaatg atcgcacccg cgtggcccat acgtttgcct ttcggcgcag
     9721 tcacaccagc gatgtaaccc acaactggct tggtaacatg ctctttgatg tacgctgccg
     9781 cttcttcttc agcgctaccg ccgatctcac cgatcatcac gatcgcttcg gtctgcggat
     9841 ctttttcgaa catttcgaga atgtcgataa agttagagcc agggatcggg tcaccgccga
     9901 taccgacaca ggtcgactga ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg
     9961 tcagtgtacc ggaacgggaa acgataccca ctttacccgg tttgtgaatg tgacccggct
    10021 ggataccgat tttgcattca cccggggtga taacgcctgg gcagttcggg ccgatcatac
    10081 gaacgcctgc ttcgtccagt ttcactttca cggtcagcat atccagcgtc gggatgcctt
    10141 cagtgatggt gataatcagt ttgatgcctg catcgatagc ttccagaatg gagtctttgc
    10201 agaacggtgc tggtacgtaa ataacagacg cggaagcgcc agtagcagca acagcttcac
    10261 gcacggtgtt gaacaccggc aggccgaggt gggtagtgcc gcctttacct ggagttacgc
    10321 caccaaccat tttagtgccg tatgcaatgg cctgttctga gtggaaagtc ccctggctac
    10381 cggtaaagcc ctggcagata accttggtgt ttttatcaat taaaatggac attatttccc
    10441 ctccactgcg gcaacaacct gctgagctgc atccgtcaga ccttttgctg caataatatt
    10501 caggccgctg tcagccagtt tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac
    10561 cacgaccggt acgttaacac ccacttccgc taccgcgccg atgataccgt cagcgatcag
    10621 gtcgcaacga acgataccgc cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga
    10681 gaggatgatt ttgaacgctt cggttacacg ttctttggtt gcgccgccgc caacgtccag
    10741 gaagttagcc ggttcgcccc cgtgcagttt aactatgtcc atcgtaccca tcgccagacc
    10801 tgcgccgtta accatacaac cgatgttacc gtccagcgca acgtagttca gttcccactg
    10861 tgcagcctgt gcttcacgcg gatcttcctg cgactggtca cgcatttcgc gcagatcagg
    10921 ctggcggaac agggcgttgc catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc
    10981 ctgtttggtg atgaccagcg ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat
    11041 ggtcgccagg cccatgaaga ttttggtgaa ctgctgaacc agtttacctt ccagacccag
    11101 tttgaacgcc agctcgcgtc cctgatacgg catcgggcca gtcagcggat caagcgcaac
    11161 tttatggatc aggtgcggag tttcttccgc cactttttcg atttccacgc cgccttcggt
    11221 ggaggccata aagaccacgc gacgggaact acggtctaca acggcaccga gatacagctc
    11281 tttagcgata tcggtcgccg cttcaaccag aatctggtta accggttggc cattggcatc
    11341 tgtttgatac gttaccagac gcttgcccag ccagttttct gcaaaagcac ggatgtcttc
    11401 tttgctgttt acaactttca caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca
    11461 tttcactacc cacggaccgg caccgatttt tgaagcggct tcttctgctt cgcgcggagt
    11521 agtacaggca taacccaccg gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg
    11581 atattcatgt aagttcatgt gttctgtcca tccttcagta atcgttatct tttaaaccgt
    11641 agaccgacac cctatccacg tcggtctaca gtgcaggtga agcttaaact actacacgtc
    11701 cagcagcaga cgcgtcggct cttccagcaa ctctttgatc gttaccagga agcccacgga
    11761 ttcgcgacca tcgatcagac ggtgatcgta ggacagcgcc aggtacatca tcggcaggat
    11821 ctcaacctga ccattcaccg ccatcggacg atctttgata gcgtgcatac ccagaattgc
    11881 gctctgcggc gggttgatga tcggcgtaga catcagggaa ccgaacacac caccgttggt
    11941 gatggtgaag ttaccaccgg tcagatcttc aaccgtcagc ttgccatcac ggcctttgac
    12001 tgccagctct ttgattttct tctcgatgtc tgccatgccg agggtatcga catcacgcag
    12061 aaccggcgtc accaggccgc gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg
    12121 gtaaaccacg tcatcgccgt cgatagacgc gttcacttcc gggtaacgtt tcagggcttc
    12181 aaccaccgct ttcacgtaga aggacataaa gcccagacgg atgccgtggc gtttttcaaa
    12241 cgcttcaccg tactgcttac gcagatccat aatcggcttc atgttgactt cgttgaacgt
    12301 ggtcagcatg gcggtggagt ttttcgcttc tagcagacgc tctgccacac gcttacgcag
    12361 gcgagtcatc gggacacgtt tttcactacg tgcagccaga gccggttgcg ccgccggagc
    12421 agccgctgcc ggagcagact ctttcgccgg ggctttcgcc agatgttttt ccacatcttc
    12481 acgagtcaga cgaccaccca caccggtgcc tttaatggcg ctggcgtcga gattgtgttc
    12541 agccagcagg cgacggatcg ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc
    12601 ctgctggcgt tgcgccggag tggacgcttt ctcttcagat ttggcgctgg tttctttacc
    12661 ggcgctgttg ccttcacgca ggcgaccaag gatctgacga gacattaccg ttgtaccttc
    12721 atcttccaga accgcatcca gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt
    12781 gtcagtttcg atttctacca gcacttcatc acgtacgact gcgtcgccgg gttttttatg
    12841 ccaggttgcg acggtggcat cggctacgga ttcaggcagg tcagggacca gaatatctac
    12901 gctactcatt gtgtatcctt tatttattcg acgttcagcg cgtcattaac cagatcttgt
    12961 tgctgtttct ggtgaacgga catataccct accgccggag aggcggaggc cgggcggcct
    13021 gcataacgca gagaagcccc aaacggaatc acttcacgga aatgatgctg gctgcagtac
    13081 catgcgccct ggttgagcgg ctcttcctgg caccagacaa aatccttgac gtgagcaaac
    13141 tgctgcaaca cttcctgcat cgctttatgc gggaacgggt agagttgctc gatacgcaca
    13201 atggcgacat cgtgttgatt gttcttacga cgctgttcca gcaggtcgta ataaacctta
    13261 ccagaacaca tcactacgcg cttcacgccc ttcggatcaa gttcgtcgat ttcaccgatg
    13321 gctggcagga aggtgccgtt cgccagttct tcgaggctgg aaaccgccag cggatgacgc
    13381 agcagggatt tcggcgacat cacgaccagc ggacgacgca tcccgcgcag cgcctgacga
    13441 cgcagcatgt ggtaaacctg tgccggggta gacggtacgc aaacctgcat gttttgctca
    13501 gcacaaagtt gcagataacg ttccagacgc gcggaggagt gctccggccc ctgcccttcg
    13561 taaccgtgcg gcagcaacat caccagacca cacatccggc cccatttctg ctcgccagag
    13621 gagatgaact ggtcgataac cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc
    13681 cagatggtca gggtgcgtgg ttctgcggtg gcataaccat attcaaacgc cagcacggct
    13741 tcttctgaca gtacggagtc ccagacacgg aacgcgccct gaccgttatg gatgtgttgc
    13801 agcggcgtgt aagtggaacc gttagactgg ttgtggatca ccgcgtggcg gtggaagaag
    13861 gtaccgcgac cggagtcttc acccgacagg cgaaccggaa tgccttcatc aaccagcgtg
    13921 gcgtaagcga ggttttccgc accgccccag tcgaacagtt tctcaccggc tgccatcgcc
    13981 tggcgatcgc cataaatctt ggcaacgcga gactgcattt caactgcttc tggtaccgta
    14041 ctgatacgtt tagccagttc ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg
    14101 tcccattcgt ggttgaggta cggcgaccag gtgaaagagt gcatgttcat cggacgccac
    14161 tctgccacta cgcaatcgcc agcatccagc gcatcgcggt acaggttaac catctcggtg
    14221 gcatcttcca gcgtcgccac tttttcctgc tccagcttgt cagcgtagat tttgcgcggt
    14281 gtcggatgtt ttttgatttt ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct
    14341 tcgttgtggc cgtgacggcg gtagcatacc aggtcgatga agacatcacg tttaaaggtg
    14401 ttacggaaat cgagcgccag acgggtcaca aaggcaacgg cttccggatc atccgcgtta
    14461 acgtggaaaa tcggtgcctg aaccatctta ccgatatcag tacagtacgg tgtagaacgg
    14521 gcatccagcg ggttagaggt ggtgaaacca acctggttgt tgataacgat acgtaccgta
    14581 ccgccaactt cataaccacg cgctttcgac atgttcagag tttcctgaac cacgccctgc
    14641 ccggtcactg cggcgtcacc gtggatggtg attggcagca ctttgttgct gctcggctca
    14701 tcaagtctgt ccagacgggc acgaacagag ccgataacga ccgggcttac aatctcaagg
    14761 tgagacgggt taaacgccag cgccaggtgc accaggccgc catcggtctg gaagtcagac
    14821 gagaagccca tgtggtattt cacgtcgcct gtgccgaggt gttctttatg tttaccggcg
    14881 aactcgtcga acaagtcttg cggtttttta cccagcacgt tcaccagcac gttcagacga
    14941 ccacggtgcg ccatcccgag aaccacttcg cgggtgccgc tgttgccagc gtggcggatc
    15001 atctctttaa gcatcgggat taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca
    15061 gggaattttg cgccgaggta acgctcaagg ccttcagcgg cggtcagttc gcttaagaag
    15121 cgttttttct cttcgctatt gaaagtcgcg cgaccagact cgatacgctg ttggatccag
    15181 cgtttttctt cggtgctggt gatgtgcata tactcggcac caatcgggcc gcagtaggtt
    15241 tgcttgaggg cggaaataag ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa
    15301 ccgacgttga aggtttcctg gaagtctgct tcggtcagat cgtggaaaga cggatccaga
    15361 tcggccactt tatcttgctg ccacagtccc agcggatcta gattcgcatg ctggtgacca
    15421 cggaagcggt atgcgttaat gagctgcagg actttaacct gcttcacatt ggtgtcaggg
    15481 tcggagatcg ttgaagagta acgtgaagcg tctttcgcca ggcggcggaa atattcacgc
    15541 gtttgagagt ggaattgatc cggtttgact cccgtaccag gtaactgctg gaacgtcgaa
    15601 cgccagttag cgtcaaccga gtcaggatcg gttaagaagt cttcatagag ctgttctatc
    15661 cagctctggt ttgcgccaga gaggtaagaa gagtccaacc aggctttcaa agcgctgttc
    15721 tgcatcgtga tcccttaagc atctttttta tgcttacttc gccgtggata ctaccacgca
    15781 cagtgatgtg cgtgccgggg ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg
    15841 aacctttaaa aactgtctta gtgtcagggg cagtttttaa agatttcctg catttctttg
    15901 ttacgcctga tgcgctgcgc ttatcaggcc tgcgggtaat gacttgtagg ccggataagg
    15961 cgttcacgcc gcatccggca ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct
    16021 acagtttacg cattacgttg caacaacatc gacttgatat ggccgatggc gcgcgtcggg
    16081 ttcagcccct tcggacatac actgacgcag ttcatgatgc tgtgacagcg gaatacgctg
    16141 aatgcatcgc tcaaaccgtc gaggcggctg tcagtctcgg tatcacggct atcgatcagg
    16201 aaacgatatg ccgctaacaa gcctgccggg ccgataaact tatcgggatt ccaccagaaa
    16261 gacggacaag aggttgaaca acatgcgcag agaatacatt catacagccc gtcgagtttt
    16321 tcgcgctgct ctggcatctg taaatgctcg cgagctggcg gattttgtcc attattcaac
    16381 aggtaaggct taattttctc atattgcgca tagaattgtc ccatgtctac caccaaatcg
    16441 cggatcaccg gtaaacctgg cagcgggcga atcacaatct tcttgcccgg ctggttgagt
    16501 gccgaaatcg gggtaataca ggccagacca ttcttgccgt tcatgttcag accgtcggaa
    16561 ccgcacacac cttcacggca ggagcggcgg aacgacaggc tgggatcttt ctctttcagc
    16621 tggataagcg catccagcag catcatgtcg cgaccttctt ccgcttccag ggtgtaatcc
    16681 tgcatacgcg gagcatcatc aacatccggg ttatagcgat aaattgaaaa ctcgagtctc
    16741 attttcctgt ctccgcatta gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc
    16801 ggttccatgt tgacgcttcg gcgcgtcatg gattccgact caggcagata cagggagtgg
    16861 cacagccagt tttcatcatc acgatccggg aagtcgaagc ggctatgtgc gccacggctt
    16921 tcggtacgga agttggcaga aacagccgtt gcatacgccg tttccatcag gttatccagt
    16981 tccaggcact caacgcgctg ggtgttgaac tcgctggaag tgtcatccag acgggcattt
    17041 ttcagacgct cgcggatcac tttcaactgc tcaagccctt tcgccatcgc atcaccttca
    17101 cggaagaccg agaagttatg ctgcatacat tcttgcagcg ctttacggat cgccaccgga
    17161 tcttcaccgt tacgattatt gttccaccgg ttcaggcgat ccagagacgc ttcaacatca
    17221 gactcgctgg catcgcgcag tgcgccctgc tcggcgatag actcttgcag atgcagacct
    17281 gccgcgcgac caaagaccac caggtccagc agcgagttgc cgcccagacg gttagcgccg
    17341 tgtaccgata cacaagcgat ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg
    17401 cctttctcat tcacagtcag cgcctgaccg gtaactttgg tcggaatacc gcccatcatg
    17461 tagtgacagg ttgggataac cggaatcggc tctttcaccg gatcgacgtg agcgaaggta
    17521 cgggaaagct ccaggatacc cggcagacgg gattcgagaa cttctttacc caggtgatcg
    17581 agtttcagtt tcgcgtgtgg cccccacgga ccatcacagc cgcgaccttc acggatttca
    17641 atcatgatgg aacgcgccac cacatcacgg cccgccaggt ctttggcgtt cggcgcataa
    17701 cgctccataa agcgttcgcc atgtttgttc agcagataac cgccttcacc acggcaacct
    17761 tcggtgacca gtacgcccgc accggcaatg ccggtcgggt ggaattgcca catttccata
    17821 tcctgcaccg gtacgcctgc gcggatagcc atgccgacac cgtcgccagt gttaatgtgg
    17881 gcgttagtgg tggactgata aatacgccct gccccgccag tcgccagcac ggtagcgcgg
    17941 gctttgaaat aaaccacttc accggtttcg atgcacagtg cggtacaacc caccaccgcg
    18001 ccatcctggt ttttcaccag atccagcgca taccactcgg agaaaatggt ggtgtggttt
    18061 ttcaggttct gctgataaag cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc
    18121 gcagtgcgtg ccgcctgctc gccgccgaag tttttcgact gaccgccaaa cggacgttga
    18181 tagatacgac catcatcgag acgcgagaac ggcaggccca tgtgttcgag ttccagaatc
    18241 gcttccggcc cggttttaca catatattca atcgcgtcct ggtcaccgat atagtccgac
    18301 ccttttacgg tgtcgtacat atgccattcc cagttatctt catgggtatt acccagcgca
    18361 acggtaatgc cgccttgcgc agaaacggta tgggaacggg tcgggaagac tttagagagc
    18421 agcgcacagg tctggccgct ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg
    18481 gcaccaatca caactgcatc aaattctctg actggcaatt tcatcacacc ccccacacca
    18541 caacgaatcc ataaatcacg taaaccacca gtgcaacgac aatcaccagc tgcagcatca
    18601 ggcgcaaggc cagcggttta acgtagtcgg tcaacacctg ccacatgccg atccaggcat
    18661 gaatcaagat agaaaacagc gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga
    18721 aaccgatcca gacttcatat gtcagttcgc cactggtggc gaaaaaaccg accatataaa
    18781 tgatgtagag cgtcaggacg atagcggtag cgcgaacgag gatgaaatca tgtacgccat
    18841 tgcgtcctaa tgcggaggcg ttgcttacca tacgaggact cctgcgagaa gtgaaagcac
    18901 gacagtaata acaaaggaga ttttggcgga gcgtttaccc gcttcgaatg tttcttccag
    18961 atagccaaaa tccatcatca tgtggcgaat acctacgacg acgtgatacg ccagagcggt
    19021 aaggatgccc cacatgataa atttgacgaa gaagctgccc ataatcgcgg aagcttgctc
    19081 gaaaccttca ggggaagaga ggctggtacc cagaagccac agcaggatgc ccactgcaac
    19141 aaaggtgatc acaccggaaa cgcgatggag aatggacgct atcgccgtga tggggaaccg
    19201 gatggtctgt aggtccagat taacaggtct ttgttttttc acatttctta tcatgaataa
    19261 cgcccacatg ctgttcttat tattccctgg ggactacggg cacagaggtt aactttctgt
    19321 tacctggaga cgtcgggatt tccttcctcc ggtctgcttg cgggtcagac agcgtccttt
    19381 ccataactgc gcgtcatgca aaacactgct tccagatgcg aaaacgacac gttacaacgc
    19441 tgggtggctc gggattgcag ggtgttccgg agacttggcg gcagtatagg ctgttcacaa
    19501 aatcattaca attaacctac atatagtttg tcgggtttta tcctgaacag tgatccaggt
    19561 cacgataaca acatttattt aatttttaat catctaattt gacaatcatt caacaaagtt
    19621 gttacaaaca ttaccaggaa aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac
    19681 ctaagattaa cttatgtaac agtgtggaag tattgaccaa ttcattcggg acagttatta
    19741 gtggtagaca agtttaataa ttcggattgc taagtacttg attcgccatt tattcgtcat
    19801 caatggattc tttacctgca agcgcccaga gctctgtacc caggttttcc cctctttcac
    19861 agagcggcga gccaaataac aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa
    19921 gttccggcag tcttacgcaa taaggcgcta aggagacctt aaatggctga tacaaaagca
    19981 aaactcaccc tcaacgggga cacagctgtt gaactggatg tgctgaaagg cacgctgggt
    20041 caagatgtta ttgatatccg tactctcggt tcaaaaggtg tgttcacctt tgacccaggc
    20101 ttcacttcaa ccgcatcctg cgaatctaaa attactttta ttgatggtga tgaaggtatt
    20161 ttgctgcacc gcggtttccc gatcgatcag ctggcgaccg attctaacta cctagaagtt
    20221 tgttacatcc tgctgaatgg tgaaaaaccg actcaggaac agtatgacga atttaaaact
    20281 acggtgaccc gtcataccat gatccacgag cagattaccc gtctgttcca cgctttccgt
    20341 cgcgactcac atccaatggc agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat
    20401 cacgactcgc tggatgttaa caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg
    20461 tcgaaaatgc cgaccatggc cgcgatgtgt tacaagtatt ccattggtca gccatttgtt
    20521 tatccgcgca acgatctctc ctatgccggt aacttcctga atatgatgtt ctccacgccg
    20581 tgcgaaccgt atgaagttaa tccgattctg gaacgtgcta tggaccgtat tctgatcctg
    20641 cacgctgacc atgaacagaa cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt
    20701 gcgaacccgt ttgcctgtat cgcagcaggt attgcttcac tgtggggacc tgcgcacggt
    20761 ggtgctaacg aagcggcgct gaaaatgctg gaagaaatta gctccgttaa acacattccg
    20821 gaatttgttc gtcgtgcgaa agataaaaat gattctttcc gcctgatggg cttcggtcac
    20881 cgcgtgtaca aaaattacga cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt
    20941 ctgaaagagc tgggcaccaa agatgacctg ctggaagtgg ctatggagct ggaaaacatc
    21001 gcgctgaacg acccgtactt tatcgagaag aaactgtacc cgaacgtcga tttctactct
    21061 ggtatcatcc tgaaagcgat gggtattccg tcttccatgt tcaccgtcat tttcgcaatg
    21121 gcacgtaccg ttggctggat cgcccactgg agcgaaatgc acagttacgg tatgaagatt
    21181 gcccgtccgc gtcagctgta tacaggatat gaaaaacgcg actttaaaag cgatatcaag
    21241 cgttaatggt tgattgctaa gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa
    21301 tttttatatg cctaaacaca aaaaattgta aaaataaaat ccactatcgg acattcatat
    21361 attctaaaaa aaaatatatc ggtataaatt ataaacaaat cgatgatttt taatcatcta
    21421 tcttcctctc agttacatga ataaaccacc aaatatagcg cactcctata atttccaata
    21481 ctttattcat caaacattta attcatacca tcaattaatt acttttacat ttaataaaat
    21541 taataatatt tttaagaaaa actaaaaaca ttcgacaatc aaataagata ttcttgcacc
    21601 gatatacttt tttcttttct gttaaaggac tcagttatgt ttaaaggaca aaaaacattg
    21661 gccgcactgg ccgtatctct gctgttcaca gcacctgttt atgcggctga tgaaggatcc
    21721 ggtgaaattc actttaaagg tgaagttatt gaagcaccgt gtgaaataca tcaggatgat
    21781 attgataaag aggttgaact cggtcaggtg accaccagcc acattaatca gtcacatcac
    21841 agcgatgccg ttgctgtcga cctgctctta gtcaactgtg atctggaaaa ctccagcaac
    21901 ggttccggtg gcaagatttc taaagttgca gtcacctttg atagctcagc gaaaaccacc
    21961 ggcgcagatc cgattctcaa caacaccagc acgggtgaag ccaccggcgt cggcgtacgt
    22021 ttaatgaata aagatcaaag caatatcgtt ttaggcaccg ctactccaga tatcgacctg
    22081 gctccgacct ccagcgaaca aacgctgaat ttctttgcct ggatggaaca aattgatcag
    22141 gctacacctg taacgccagg cgcagttacg gcgaatgcaa cctacgtgct ggattataaa
    22201 taagtattac tttggcggcg ctatcgccgc ctttcataaa atatttatca ggtatggaca
    22261 ccgtgaatat ttatcgactc tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat
    22321 tggcggtcga attcaatctg aatgttctcg ataaatcaat gcgcgaccgc attgatattt
    22381 cattattaaa ggaaaaagga gtcattgctc ccggtgagta ttttgttagc gttgcggtaa
    22441 ataataacca aatcagtaac gagcaaaaga ttaactggca caaaaatgac gataaaacca
    22501 ttccgtgcat caatgattta ctggtcgata aatttggctt aaaacctgaa gtccgtcagt
    22561 cattaccatt gataaatcag tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt
    22621 tcgatcaagc caatcagcaa ctgaatatta gtattccgca agcctggctg gcgtggtact
    22681 cagaaaactg gaccccaccc tccacatgga aagaaggtgt cgccggtgtc ctgatggatt
    22741 acaacttgtt tgccagcagc taccgcccac aggatggcag cagcagcact aatcttaacg
    22801 cctatggaac agccggaatc aacacggggg catggcgctt acgcagtgat tatcagttga
    22861 atcatactga tagcgatgat aaccatgaac agtcaggcga aatatcacgt acctatcttt
    22921 ttcgcccgtt accgcaatta ggttcaaaat taaccctcgg cgaaacggat tttagttcca
    22981 atattttcga cggtttttct tataccggcg cggcactggc aagtgacgat cgaatgttgc
    23041 catgggaact acgcggctac gccccacaaa ttagcggtat tgcacagacc aatgccacgg
    23101 tgacgatcag tcaatcaggc cgcgtcattt accagaaaaa agtcccacca ggcccattta
    23161 tcattgacga ccttaatcag tctgttcagg gcacactgga tgtcaaagtg acggaagaag
    23221 atggtcgggt gaacaatttc caggtttcag cagcatcgac gcccttcctg actcgtcagg
    23281 gacaggttcg ctataaactg gccgcgggtc agccacggcc ctccatgtca catcaaactg
    23341 aaaatgaaac cttttttagc aatgaagttt cctgggggat gctgtcaaac acctcgctgt
    23401 acagcggcct gctgctttct ggtgatgact accattctgc cgcaatgggt attgggcaaa
    23461 atatgctgtg gcttggtgcg ctgtcgtttg atgtcacgtg ggccagtagc cattttgata
    23521 ctcagcagga cgagcggggc ttaagctacc gttttaatta cagcaaacaa gtggatgcca
    23581 ctaacagcac gatttccctc gccgcttatc gtttctccga tcgtcatttt cacagctacg
    23641 ccaactatct ggatcacaaa tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca
    23701 gcttatctgt gggccaaccg attaccccac taaacctcaa tctttacgcc aacctgctac
    23761 atcaaacctg gtggaatgca gacgcctcca cgaccgccaa catcacggca ggttttaatg
    23821 ttgatattgg tgactggaga gatatctcta tttctacgtc attcaataca acccattacg
    23881 aagataaaga tcgcgacaac cagatttacc tgtcgatttc gctccccttc ggtaacggtg
    23941 gtcgggttgg ctatgacatg caaaacagta gccacagcac cacacaccgc atgtcgtgga
    24001 acgatacgct ggatgaacgt aatagctggg gcatgtctgc cggactgcaa tccgaccgtc
    24061 ctgacaatgg agcccaggtg agcggtaact atcagcacct gagttcagcg ggtgagtggg
    24121 atatttctgg tacctatgcc gccaatgatt acagttccgt cagcagcagc tggagcggtt
    24181 ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac
    24241 gcctgatggt cagcaccgat ggcgtggcag atattccggt tcagggcaat ctcgactaca
    24301 ccaaccattt tggcattgcg gtggtgccgt tgatttccag ttaccagcct tccaccgtgg
    24361 cggtgaacat gaatgactta cccgacggcg taacagttgc agaaaacgtt atcaaagaaa
    24421 cgtggattga aggcgcgata ggttacaaat cactggcttc ccgttccggt aaagacgtta
    24481 acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct cggagcggat atccgccagg
    24541 atgacagcgg cattagcgtg gggatggttg gcgaggaagg acatgcctgg ttaagcggag
    24601 tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag ccagcattgc tcgctccatc
    24661 ttcctgaaca tatggaagac accgcaaatc gcctgatttt accttgtcat taattaagga
    24721 aatgaaaatg acatttatga aaggactacc ccttttgttg ttggttgcca gtctgtgcag
    24781 ccacgctgca ctacaacccg atcgcactcg tattgtgttt aacgctaatg ataaagctac
    24841 cagtctgcgg gtggataatc gtagcgataa actcccctac ctcgcttatt cctggctcga
    24901 aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc ctgccgccga tacaacgtct
    24961 ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa gcctcgacca caaaattacc
    25021 gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa
    25081 aaacagcgac catgccgttc tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg
    25141 accggctgca ttacgcaaga aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca
    25201 gcagggcaac caactgacac tgaaaaatcc taccacgtat tatctgacca ttgcctacct
    25261 gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc gtgatggttg cgccttttag
    25321 tacggtcaac acaaacaccg gaaattatag cggcagccag ttttatctcg gttatatgga
    25381 tgattacggc gcgttacgca tgacgacgct caactgcagc ggacaatgcc atttacaagc
    25441 cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc tcgtcatctg tctgttattt
    25501 ctgccgttga agtctgcgct tgccctgaac tgctactttg gttcatccgg tggctcggta
    25561 gaaaaatcag aagcgattca accttttgct gtaccaggca atgccaaact cggcgataag
    25621 atctgggaat ctgacgatat taaaattccc gtctattgtg acaacaatac caacggcaac
    25681 tttgaaagcg aacatgtcta cgcatgggta aacccctacc cgggggtgca ggatcgttat
    25741 tatcaactcg gagtaactta caatggcgtt gattatgacg ccaaccaggg aaaaagccgc
    25801 atcgacacta accagtgtat cgacagtaag aacattgaca tttatacccc tgagcagatc
    25861 atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg
    25921 cgtacctttc tcgctcgcat gcggttatac gtcaaaatac gagaaatgcc gccgcatgat
    25981 tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac
    26041 gaagacccta ctgcccaaaa cctgaaatat catattactg gtctggaaaa cattcgcgtg
    26101 ctggattgca gcgtcaattt ttccatttca ccggaaacac aggtgattga ttttggtaaa
    26161 tttaatttgc tggatattcg tcgccacaca atgtcgaaaa cattcagtat taagacgaca
    26221 aaatcacaaa atgaccaatg caccgacgga tttaaagtca gttcctcctt ttataccgaa
    26281 gaaacgttgg ttgaagaaga taaagcattg ttgattggta atggtctgaa gctacgtttg
    26341 ctggatgaaa acgcctcgcc ttataccttc aataaatatg ctgaatacgc cgatttcacc
    26401 agcgacatgt tggtctacga aaaaacctat acagctgaac tttcgtccat tgcaggtacc
    26461 cccatcgagg ctgggccctt cgatacagtg gtgcttttta aaattaacta taactgattt
    26521 gccatgatgc cctttgcagc cggaaagggc atctctttca gaaaattgct gattttcccc
    26581 ttttccttga ttgctgttat tttttcctag cccggctttc tttatgctgt atcgatacac
    26641 caaagagaaa ataatgaggg agcgaaggat gccagttttg cagtggggaa tgttatgtgt
    26701 gctatcactt ctcctttcta ttggtttcct cgcggtacat ctcccggcgg cgctattact
    26761 tgggccgatg atcgccggga tcatcttcag tatgcgcgga attactctgc aacttccccg
    26821 ctccgctttt ctcgccgcgc aggccattct tggctgcatg attgcgcaaa acctcactgg
    26881 ttcgattctc accacgctgg ccgccaactg gccgatcgtg cttgctattt tactggtgac
    26941 gctgctttcc agcgccatcg tgggttggtt attggtgcgc tatagctcac tgcctggaaa
    27001 taccggtgcc tggggttctt ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga
    27061 ttacggcgca gatattcgcc tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc
    27121 gggggccgct gttctggtca cccgcatgat actgggtgat aacgctgaag cggttaacca
    27181 gcaaatcgtc tggttcccgc cagtgagcat taatctcctg cttacgattt tgctcgcagt
    27241 cgttgccggt acggcgggat tcatgctgcg tcttccttcc ggcacgatgc tcatcccgat
    27301 gctggcgggc gcagtgctcc agtctggtca gctcatcacc atcgaactac cggaatggct
    27361 gctggcgatg gcgtatatgg caattggctg gcggattggt cttggtttcg ataaacaaat
    27421 cttactgcgg gcactacgcc cgctaccgca aatcctgctg tcgatttttg ctctgctggc
    27481 tatttgtgca ggtatggcgt ggggactgac ccggtttatg catattgatt ttatgactgc
    27541 ctacctcgcc accagtcccg gcgggcttga taccgtggcg gtcatcgccg cagggagcaa
    27601 tgccgatatg gcgcttatta tggcgatgca aaccctgcgc ctgttcagta ttttgctgac
    27661 agggccagcc attgcgcggt ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg
    27721 gcagcctgga caccagtaaa acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc
    27781 acagcgttcg cacagttcgc catcacgatg aaaaacctta aagcgaaaca gcgccccatg
    27841 atgcttattc tcatccacct gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa
    27901 cgcgtgtgcg agtgcatcca gttgcgccgc attgagatct ttcgctttat gatttccagt
    27961 caacccaacc tgccagagga tctccacccg caaataattg ccaagcccag ccagaaacgc
    28021 ctgatcgagc agtaatcctg caaactgacg gttacgaaag cgcggcgaca ataaccgttc
    28081 ctttaccacc tccggcgtca gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa
    28141 cggatgcgtg gtcagttgtt ccggcctcaa catctcaata tccgaggcgc tataaagcag
    28201 aatggttttg tcagccgttt gcagttttac ccgcaatact cgcgtggtct gcggctcttc
    28261 gccggtatca accacgcgcc agacgccgta aagctgatta tggctgtaga gcgttaaacc
    28321 gttgggaaaa tgagttaaca acgccttacc acgcgtttcc acatgggtaa cgtgttgacc
    28381 gataagttgt gattgataag tttttaactg cgggaaggca aaccagacat cagttagtgg
    28441 tttgcctttg atcgccgcct ccaggttatc cgctgcacgg cggatctccg ggccttcagg
    28501 catggtgata tccttttttc attctttatt aagcggggtt atgctgcgac aacaatcccc
    28561 tttttggcaa atgtagctcg taatcggcgg gcgaacgcca gagcgtgctc gccgtcgcca
    28621 tgcaggcaga ccgtttgcgc cgcgaccgtt gcccattcgc cggtgatact tttgactctg
    28681 ccgtgttgca ccatctccag cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg
    28741 cctggctgac ttcgcggcac cagcgagccg tcagcctgat aaccgcgatc ggcaaacact
    28801 tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa tcagctcgct tcctgccagc
    28861 ccaacgagaa ccaataccgg atcgcaagcg tataccgctc tggcgatggc gtctgccagt
    28921 tgtgtttctt tcgccgcctg gttgtacaac atgccgtgcg gtttgacatg acgcattacg
    28981 ccgccttgcg cacgggcaat cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa
    29041 acggtttccg gcggcagctg catggcgctg cgaccaaaat tttccctgtc gggaaaactc
    29101 gggtgagcgc caatcgcgac accatttttt attgcttcac gcacgcaagc ctgcatggtt
    29161 tgcgcatcgc ctgcatgaaa tccacaagca atattggcag aggaaaccag cgttaatagc
    29221 tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag
    29281 ccgccacgct aattgttcaa aataacgttg ctgatcttgc cgcgctttca gtgcctcttc
    29341 cagtgaacac tggacaaaat gaatcggctg accgagcgga atttgcgcca gatggtacat
    29401 atcagcctca atgatacagg caatacgtgg ataaccaccg gtggtctgtg cgtcgttcat
    29461 caacacaatc ggctgcccgt tatgcggcac ctgcaccacg cccggtaaca aaccgtgaga
    29521 taacagttcg cgatcggtga tgcgttttaa aatttgcccc tgtaaacgat agcccattcg
    29581 gttactttgc gagctaagct gccagggcga acgccagaat gcatcctgcg aggcgcgatc
    29641 gaactcatga tattccggcc ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt
    29701 aacgccctgc gcttccataa aatcacgctt cgatttgcca atcgggagtc ggtcaccatc
    29761 cttcagtaaa cggccttcca gcccgccaat ccccactttg agatcggtgc tgcatgaccc
    29821 cattaccggc ggaacatcaa taccacccgc gaccgccaga taactgcgca tcccgtgctg
    29881 cgggcgttta agcgttaaac gctggcctgc tttcatcggc aatcgccagc cggtccagac
    29941 ggcgttatca tccaaccgcg cttcgcaacc tgcacccgtc agggcaaacc acccatcagt
    30001 ttcgaactca actgttaact gaccgagcgt gatctccagc gcgggggcat tggcgtcatt
    30061 acccaccagc aggttagcaa tgcgcagcgc aggcatatcc agtgcgccac agtggctgat
    30121 acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca gtggtgtaca tgcccgcacg
    30181 aataatcttc agcatattcc ctccttctgc ggtacaaatc gcacgctgtc tcccggacgt
    30241 aataagatgg gttcgtcacg cgccggatca aacaggctga gtgaggtatg accaatcaac
    30301 tgccagccac ccggcgttgc cagcggataa acaccagtct gcggcccgcc gatcccgaca
    30361 gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc
    30421 aaactcccga gatacgggaa gcccggttga aaacctaaaa accagaccac gtattccacg
    30481 gaggagtgca attcaacaac ctgtttttcg ctcaacccgc aatacgccgc gaccaccgcc
    30541 aaatccggtc cgcctgcacc accgtaaacc accggaattt caataaagcg agactcaggt
    30601 tccagggcct cgctctcctc ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc
    30661 gactcaggat tacgcaaaat caccgtgata ttgttcatgc cgggaatggc ttcaaccaca
    30721 ttcggcatat ccaccagacg ctgcgccagt cgccagatcc gcttctggct agccagcgtc
    30781 accggcggtt ccagttccag cactaccgcc gtttcaccta tcagataaca acgcgctcgt
    30841 tgcactttcg atccctctga ttattcgtta tgcaggatta ggaatatcaa taaaggtcac
    30901 atcaagatcg gtattttcat tcagccactc gctcaatgcg cgaataccac cgcgttcagt
    30961 ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg
    31021 ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa
    31081 actttgcccg ccgcccgtgc accaggcgac gcgctggacc acctcaggtc cggtatcgcc
    31141 acaccataac ggcttacgtc ccagacgcgc ttcaatccaa gaggctagtt ccattcccgg
    31201 cacaggcatg gtcagctctc cccacggcac caacggctca atttcgccca tgaccgtgat
    31261 ccccagtaac gccgccagct gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa
    31321 atgccagcca tacaggttga tatcattctc cagcaacgtt tttaaacggt tacgcttcat
    31381 tccgcgaatg accggagact ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc
    31441 cgcgcccaga cgcactgcct catcgagcaa agcctggctg gcggtgacac cggtaacaat
    31501 tttttgcacc gtctctttgc cttccacctg caaaccattc ggcgcgtaat cactaatcgc
    31561 cgcgctgttc agtttttcgt taatcagttg ttccagttcg gtgtttttca tcttcgcgcc
    31621 ttccgtattc tgatagctac accgttaaca tagcgttacc tcgcctgttc gtcgatagcc
    31681 aatcttttga catattaaga aacaaacccg acgccaaaag acgcaaattg tttatatcat
    31741 cacgcttcag ctaatgtata tttatgcaat tagacagcat attgcataaa taatcagatc
    31801 tgtatcaaag ttttaacttt aaggaaacac aagcacgccg cgcaactatt acgtgacgtt
    31861 gccgtcaatt gtggaattat tatgaataaa cacgcatcac aaccgcgcgc tatttactat
    31921 gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg gcatgcgtgc cctgctgatt
    31981 ctctatctca ccaatcaact aaaatacaac gatactcacg cctacgagtt atttagcgcc
    32041 tactgttcgc tggtgtatgt cacgccaatc ctcggtggct ttttggcgga taaagttctc
    32101 ggcaatcgca tggcggtgat gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg
    32161 ggtgccagtg agatccatcc gtcattcctc tatctgtccc tggcgattat cgtctgcggc
    32221 tatggcctgt ttaaatctaa cgtaagctgt ctgctcggcg agctgtataa gccaaccgat
    32281 ccgcgtcgtg atggcggttt ctcgctgatg tatgcggcgg gtaacgtggg gtctattatc
    32341 gcacctatcg cctgtggtta cgcccaggaa gagtacagct gggcgatggg ctttggcctg
    32401 gcggcggttg gcatgatcgc gggtctggtc attttcttat gtggcaatcg tcatttcact
    32461 catacccgcg gcgttaacaa aaaagtactg cgtgcgacaa actttctcct gccgaactgg
    32521 ggatggctgc tggttctgct ggtggcaacg cctgcactga ttaccgtgct gttctggaaa
    32581 gagtggtcgg tatacgcctt aattgtcgcg actattattg gcctgggtgt actggcaaaa
    32641 atttatcgca aagcagaaaa ccagaaacag cggaaggagc tggggctgat tgtgacgctc
    32701 accttattca gtatgttgtt ctgggccttc gcacaacagg gcggtagctc gattagcctt
    32761 tatatcgacc gcttcgttaa ccgcgatatg tttggttata ccgttccgac cgcgatgttc
    32821 cagtcgatta atgccttcgc agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa
    32881 gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa aatttgctct tggccttggc
    32941 ctgatgagcg ccggattctg cattctgacc ttaagcgccc gctggtccgc aatgtatggt
    33001 cactcttctc tgccactgat ggtgttaggc ctcgcggtga tgggctttgc ggaactgttt
    33061 atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa ttccgggcgt gaccggcgta
    33121 ttaaccggca tctacatgct gctttctggc gcgatcgcga actatctggc gggcgtgatt
    33181 gccgatcaga catcgcaggc ttcgtttgat gcttccgggg cgatcaacta ctccatcaat
    33241 gcatatattg aagtatttga tcaaattacc tggggcgcac tggcgtgtgt aggactggta
    33301 ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca
    33361 taataggcca gcaccgatgc ctgatgcgac gcttgacgcg tcttatcagg cctacgtatt
    33421 tcctgcaatt tattgaattt gcacaaattt gtaggctgga ttaggcgttc acgccgcatc
    33481 cggcatctgg gctcgattgc ctgacgcgtc tgttatttcc ccttccgcgc cgcctcatac
    33541 gctgccaacg tttgtactct cgcctctttg tgctcgacta tcggttgcgg ataatccagc
    33601 ttcacacctg ctttctgcgc ccacttccac ggctcatgca ccgctttccc tggtacatcg
    33661 cgcagctccg gtaaccatcg acgaataaac tcgccctcac ggtcaaattt ctcgccctga
    33721 gttataggat tgaaaatacg aaaatacggc gcagcatcag tacccgtaga agccgcccac
    33781 tgccagccac cgttattagc tgccaaatca ccatcaatca gctgcgacat gaaatatcgc
    33841 tcgccttcgc gccagtcgat caacaaatct ttcaccagaa aactggctgt aatcatccgt
    33901 agccggttat gcatccagcc agtgctgtta agctgacgca tagcggcatc aataatcggg
    33961 tatcccgttt tgcctttctg ccaggcctgt aaatgtgcgg gattgctttg ccactgcaca
    34021 cgatccgtcc aggcaataaa tggacgatgt ttacacaacg aggggtaata cgtcatcaga
    34081 tgacggtaga actcgcgcca gatcagctca ctaagccaga cactaccggc cccaccgtcc
    34141 agcgcctgcg gctgttccgc caacaagcga tgcaaacact ggcgaggcga taacccgccc
    34201 gttgccaagc tggcggacaa acggctggtg ccttccactg ccggaaaatc tcgttgttgc
    34261 tcatactctc cagcaccgtt ctcgcaaaac tggcgtaatt gcgcaatcgc cgctttttct
    34321 tccaccggga aatgcgcagt atcgaaagac tgacgaggat aattcagcgt aatggatggc
    34381 gcgggcttta tcgatccgct actacgaact tttggtgcag cgacgcactc cggcatccct
    34441 tcccgcagcc gtttcagcca ggcattctta aaaggcgtaa agactttgta catctcgtga
    34501 ttgccggtca tcaccgcccc cggcggcagg atcacgctgt catcaaatcc ttcacacacc
    34561 acgttacgca gcgctctttc aacgtccaca tcccgcgctc gctcattcac ctcatactga
    34621 tagttataaa aaagatgagt aacgctgttt tccgcgcaca cctgtttaac tatttcgaca
    34681 ctggcgacaa agtcatccac ttcacggaac aataaaggaa tacctttttc cgccagcgcg
    34741 atttgtagcc cattcagttg agcattgata agttcagcct gacgcggcga catgttatgc
    34801 gtctcccact ggcgtggtgt agcgatatac agcgccagca cgcgtgcaga cgaattgcgg
    34861 caggcggcag ccagtgcgag attatcgtgc agacgtaaat cctggcgaaa ccaggccaga
    34921 tgggtagtca taaaactcct gaatggcgca agttaatcac acgcggccaa agttcgaaat
    34981 aacgctgccc tgaaagccag gcgtcaggat aaagcgccat atagtgtttg atacgcatca
    35041 gcggcgcaag aagtggttgc tcgccacggc gataactgtc gataagcgca gccagctgct
    35101 ggcgttctgt ggaatcaata tgcgggcgaa aataaccctg aacgtgcatc aaaacgttgg
    35161 tgtgatcgcg cgggttggcg ggatgagaaa gcaaaacaat tacgcgttgg cggtactggt
    35221 tgtaaaaatc atccagattt tgccactgat gaatatcagc cacaaacggg ccaagtttgc
    35281 ggtactcggg ctgagaatgg gccagaaaga cgagtttata gcggctgtga taatcgagaa
    35341 gcgccccgcg cgttaagcca tgttctttca gtcgatgtaa ctcatgaagg gcgaagatac
    35401 ggattagggt gctgtcatca aatcgttgta gattcattac tgctcctgaa cggtatacag
    35461 gagtaagtgt agacgttggt aaatgatgtt tgtgaagtta atccgctagc agggcaagat
    35521 tacgaaagcg cgctccccgc aagcaactaa tcaaggtagt taatcatggc tgaacacgat
    35581 attacgccag aagtcaccat ttccaaaaca cagcgccgct ataaagtcgg ctatgtcaga
    35641 gcccgccacc aggaccgcaa aaccggtata gcccggtatt atagcctgca ccttaagggc
    35701 gactggctga aagaggccgg atttgatacc ggacggggtg tcacggtgaa gatttcggag
    35761 gggggcctga ccatcatcgc tgacagtgac gaggtgcagg aactgcggga ggaactttat
    35821 caggttaaac aggtagttaa ggggatgcgg gatgggatgt ttagtgtgct gaacgaaagt
    35881 taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc tgtcttatta
    35941 taatgtatac tcatcatctt ccgatgaatt tataaattgc tccaagttta catggagagt
    36001 acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc caacataatc
    36061 atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata ttcgtagttt
    36121 aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta tcatagcata
    36181 ttcctctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa tttcaatcca
    36241 tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc cacttaataa
    36301 tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct aacacttcca
    36361 ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg tccatgacct
    36421 gacactctat atggaggttt tgttacagaa ggatctttta tatcatccca atgatatgaa
    36481 cctgcttttg ttttatttcc agtaagatat tccattaagt tcttagtgct ggggcctgac
    36541 tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa ttgttcagcg
    36601 tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg attcgctttt
    36661 tggttactaa cagcacatgg agttaatcca agtggatcag catattttag aggattaggt
    36721 gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac catgaaccgc
    36781 ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc cctgtcgagg
    36841 tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc ctgtatcgtg
    36901 ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc cgtcatctct
    36961 tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc ctctttgccc
    37021 gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat gtacaggctg
    37081 cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa aaattttgtc
    37141 cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct ccccagtgca
    37201 tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg gtgttccgca
    37261 tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg gccaaattcg
    37321 tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac cacgctgttt
    37381 tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc gtcgtactgc
    37441 cagctcgcca gatacccctc atcctccgaa cgacacgccg tgatgctccc cgtggggtcg
    37501 tagaagtaat ccgtttcccc ccgccggctg tgccgcgtct tcagcagttc atcctcgcca
    37561 ctgtagctgt actgccggtc cgtgaacgtc tccggtacca ccccgcgcat cccgctgtat
    37621 gtctccctgc gcttcagccg cccgccgggg tcgtaaagct gccgcgtgct cagtcgcccc
    37681 tggcttctga gcacttcacg gtgcaggtgg tcgcgcgtga actcgctgat gacctcccgg
    37741 tccagattta tctgctggag atggccgctg ccgtagtaca gatggcgctg cgttctgccg
    37801 tccggaaagg tggtggcagt ccggttgccc agttcatcgt actggtgggt caggatgccc
    37861 tgcgccgtct cctcgctcag catccgcccc agtctgtcgt agctgaagcg gataacttcc
    37921 ggcgcgtcag tttcgtgccg ctggcgacgg atttccagta gctggtctgc cgcatcatgg
    37981 acgtattccg tacggccatc ggctgtctct tttgccgtga gccggcccgc cgcatcacgc
    38041 gccagccggt ggcggatttc accgccccgg tccccggtga acaccacttc cgtgaccgcg
    38101 ttcagcgcgt tgtagcggta cgactggcgg cagccgtccg ggtttatctg ctccgccaga
    38161 cggtccagca catcatggcg gaaccggtag cgttctccgt tctcgttcgt gagcgtgacc
    38221 agacgcccgt aagcgtcgta cccgaatgcc gtcctgcgcc ccatcgcgtc cgtccggctg
    38281 cgcacccggc cctgcccatc ccgctgccag cgcgttatcc tccccatcgg gctggtgtac
    38341 tttaccagcc tgcccgccgc gtctgactgg taattcaccg tgcggccgtc cgccagtatc
    38401 acctccgtca gatgaccgct gtcgtcatac ccgtagcgcg tgctgtggct ctgcgcatcc
    38461 gtcattctga taagcctcgt gcgttcgtcg tagaaccacg ccgtctgcga gccggaacag
    38521 tccgtgtgcc gcatgagctg cccgtcttca ttccactgga ggtatttatt ccccccgcgc
    38581 gcatctgtta tctgcaccac ctggccgtgg tggtcatact gataccgggt gttctgctgc
    38641 agcgggtcgg tcacggcaag aaggttgccg cgttcatcgt acacacagcg ccaggtgttt
    38701 cccgccgcgt ccacctccgt ttccggctgg tgccacaccg ggtgccactg cgcctgctcc
    38761 acccgcccca gcgggtcgtg cgtctccgtg atatgcccct gcaggtcata cacatagcgc
    38821 catttgccgc cctgagggtc ggtcatcccc agcaacagtc gttcttcgtc gctccagcgg
    38881 aatgtcgtca cctgtccggc ttcgtcctgg tacgccgtca cctgattctg cgcatcccac
    38941 tggtgataac gtgtgcgccc cagtccgtcc gtcacgcaca gcctgcgctg cgccaggtcc
    39001 cacgccagcc ggtactgttc accgctgtcc gtgtggtgcc ccgtcacccg ccatgccgcg
    39061 tcatccggac cttcatacca gccccactgg taataatacc cttcgccgcc cggctggcgg
    39121 tgcagcgtca tgcagtggtt ttcgtcgtaa gcaaactcac gcagcaggtg ccccgtgctg
    39181 tcgtacacgc cgttcaggtg gccggcctcg tcatagctgt aacgacgcag cagtaaaggt
    39241 gcagtctcag ggaaatgctg ataaatctgt gtcacccgcc ggggatgcgc cgccagttcg
    39301 taatacagac ggatgcacgg acgctgctgt tcgccgctga tttccacaat gcggccccgg
    39361 tcgtcgtagt acagattcag acagttgctg ttacggtcgc ccagcatttt cagccgctgg
    39421 cgctgcgggt ggtgcgggtc agcctcaaac agaaaacact gcccgtcatc gctgctgata
    39481 agccagtgcc cctgctcacc acgccttacc gccagccctt caccggggct gtaaaacccg
    39541 ccccccggca gtacggcttc catatccagg cgtcgcccca tgcccgacac aaaggtcatg
    39601 cagttttcgt ctgcgctacc cggtgtgcac acgaggctga cttcatacat tacactccag
    39661 ccctgaccaa acattccttc cgtacgttcg tcacgactgc tgtacacgcg ctgccagtcc
    39721 agcggaaagt ggcccggcag actgaaatcg aagtcatcgt catcggccag gtatttggcc
    39781 ccggtggcgg cattgaccgg atgcggaagg gcactggtgg ccttctgcgt cagcatgctc
    39841 atcccgacac tggcagcaaa acaggcgatt ttagaaagca ttttgcccgg gcgaagaaac
    39901 atcagcgcca cggttgccac cagggttatc tgcgacctgc cgcttctgat atccctgacc
    39961 accagtaacg gaccgccaat gcgcacatcc ggtgacacaa acgccccgtt ccccggctca
    40021 ttcaccaccc tggcctcgca ggtacagcgt gccccgctgc gtgtcgccgg ctggctgttg
    40081 atacgcacat cccgcgcgcc ttccgcgata tattccgctg cgggcggctc gtcatcgcct
    40141 ttgaacagat tactgatgct gccaataacc gcattcactc cctgaaaggc ggtttccagc
    40201 ataccgcccg gattcagcag gttaagggcc gttcccaggg ggtcatccag aaaggcggct
    40261 tttttttgtg cgagaaactg cgtcagtgag gaaggatgtt tatggcagtc gattttatcg
    40321 tcaggggtcg gggtggagcc cggggccgca ctggccacag tgggctgcca tagttccgac
    40381 acaaaatgtg tcacgccatc caccatttca gaacgccctt cttccgtact gagctggtcg
    40441 tgcagagccc gggacatcag cacaggccct gtcaccgggt taaccgccag ggctatggcc
    40501 cccgccattt ccagatattc cccggccaca tcccggaacg ttctggcatc ctcctgacgc
    40561 tgcgcttctt cctgttctgc cttcgcctgc gcttcctgcg cctctatgtc ctgccgcgat
    40621 gccgcagtcg ccgctgcccg cgcggcaggt ttgctgttga tccatgtgtc accagagccg
    40681 gtgaggatgt aaccttcaat ctgaggcggg aaaagcatat ttgcgagggc tgaacaggct
    40741 tcgctgacct tttctgtcac gccggtgtac accatcgcca cacccgccac aaaaccggtc
    40801 agcacgacgg ccgtgccccc catcgtgatc cccactgacg tcgccagtaa cgaggttgtg
    40861 ccgacaatga gggcatcaat aacgacattt gcagccacct ccagcaccgc ccccagcaca
    40921 tctgccataa acgacgtatg cagcagggca tccccctgtc ttgccgccca gaatgcctct
    40981 tccatgtccc gttccctgta tgattaattc gtcctgactt cctgccgcat cctgaagcgc
    41041 agattctttt taatttcagc ccagcgcgtc atatctgccg gggtcagcgg tgtggttctg
    41101 gcgtaggtga aggccatcat gacccgctca cccggcaata caaatgccac ctgacactgg
    41161 tacacctgct cgttaccccg gagaaactgg ctctgtattt ccaaaccgcc tcgttgagat
    41221 aatattccgt cataatgtaa atagtccgtt ttaataaagg aattatttat tgacaggttc
    41281 tgttttttta atcatgaatg taatacccat caccgcttta cagcacactt tatctttcca
    41341 gccataatca ctaataatat taagcaatta gcaatattta caacgaccag agcggataca
    41401 tagttatgta tttaacaatc ccaatatatt cactatgcaa ataaaaccac tagtaacaga
    41461 cacgctaccg cctccatata aaaatggaca ccttataaaa ttgtatcttg catagaaaaa
    41521 gctgaaatat cggatgcgtt ttaattatca actataaatc tataagcttt ttattaacaa
    41581 ca
//
LOCUS       DADMFL010000021        40649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.021, whole genome shotgun sequence.
ACCESSION   DADMFL010000021 DADMFL010000000
VERSION     DADMFL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.021"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            231..1664
                     /locus_tag="JNZ27_001151"
     CDS             231..1664
                     /locus_tag="JNZ27_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY8663565.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNZ27_001152"
     CDS             complement(1672..2730)
                     /locus_tag="JNZ27_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8663566.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLALRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNZ27_001153"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNZ27_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNZ27_001154"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNZ27_001154"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY8663567.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNZ27_001155"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNZ27_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY8663568.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNZ27_001156"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNZ27_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY8663569.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNZ27_001157"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNZ27_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY8663570.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNZ27_001158"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNZ27_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY8663571.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGTIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNZ27_001159"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNZ27_001159"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY8663572.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNZ27_001160"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNZ27_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY8663573.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNZ27_001161"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNZ27_001161"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY8663574.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNZ27_001162"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNZ27_001162"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY8663575.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JNZ27_001163"
     CDS             complement(17699..18322)
                     /locus_tag="JNZ27_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY8663576.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELINEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JNZ27_001164"
     CDS             18619..18894
                     /locus_tag="JNZ27_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663577.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHLVR"
     gene            complement(18971..19213)
                     /locus_tag="JNZ27_001165"
     CDS             complement(18971..19213)
                     /locus_tag="JNZ27_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY8663578.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JNZ27_001166"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JNZ27_001166"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY8663579.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JNZ27_001167"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JNZ27_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY8663580.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JNZ27_001168"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JNZ27_001168"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY8663581.1"
                     /translation="MADTRPERRFTRIDCLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /locus_tag="JNZ27_001169"
     CDS             22592..24289
                     /locus_tag="JNZ27_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY8663582.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..25038
                     /locus_tag="JNZ27_001170"
     CDS             24304..25038
                     /locus_tag="JNZ27_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8663583.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25051..25908
                     /locus_tag="JNZ27_001171"
                     /pseudo
     CDS             25051..25908
                     /locus_tag="JNZ27_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25911..28412)
                     /gene="ptsP"
                     /locus_tag="JNZ27_001172"
     CDS             complement(25911..28412)
                     /gene="ptsP"
                     /locus_tag="JNZ27_001172"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8663584.1"
                     /translation="MLTIQFLCPLPMPNGLHARLARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLGKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28437..29474)
                     /gene="ypdE"
                     /locus_tag="JNZ27_001173"
     CDS             complement(28437..29474)
                     /gene="ypdE"
                     /locus_tag="JNZ27_001173"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY8663585.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSGMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29474..30557)
                     /gene="ypdF"
                     /locus_tag="JNZ27_001174"
                     /pseudo
     CDS             complement(29474..30557)
                     /gene="ypdF"
                     /locus_tag="JNZ27_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30572..31859)
                     /locus_tag="JNZ27_001175"
                     /pseudo
     CDS             complement(30572..31859)
                     /locus_tag="JNZ27_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(31881..32207)
                     /locus_tag="JNZ27_001176"
     CDS             complement(31881..32207)
                     /locus_tag="JNZ27_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY8663586.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32426..33391)
                     /gene="glk"
                     /locus_tag="JNZ27_001177"
     CDS             complement(32426..33391)
                     /gene="glk"
                     /locus_tag="JNZ27_001177"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY8663587.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33595..34851
                     /locus_tag="JNZ27_001178"
     CDS             33595..34851
                     /locus_tag="JNZ27_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY8663588.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34966..35292
                     /locus_tag="JNZ27_001179"
     CDS             34966..35292
                     /locus_tag="JNZ27_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8663589.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35433..36671)
                     /gene="mntH"
                     /locus_tag="JNZ27_001180"
     CDS             complement(35433..36671)
                     /gene="mntH"
                     /locus_tag="JNZ27_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY8663590.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            37007..38209
                     /gene="nupC"
                     /locus_tag="JNZ27_001181"
     CDS             37007..38209
                     /gene="nupC"
                     /locus_tag="JNZ27_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY8663591.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38259..40448)
                     /locus_tag="JNZ27_001182"
                     /pseudo
     CDS             complement(38259..40448)
                     /locus_tag="JNZ27_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10824 a        10115 c         9412 g        10298 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg agggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acacggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtagaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctccctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggca ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatattgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgctacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ccttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattgat cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcttgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acaatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaataa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccgt atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt
    24721 aatctggtga aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa
    24781 gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac
    24841 attaccgaat tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt
    24901 tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg
    24961 cgactgaaag atctggattt tgaagtgccg gtcagccgca gcaaagtgaa agaatttcgc
    25021 cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa
    25081 caatttttcg ccgatctgtt cagcggccta gtgcttaacc cgcaactact ggggcgcgtc
    25141 tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt tatgtattga ttttccccgt
    25201 ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca gcagcgaatg
    25261 gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccgat caacaacaaa
    25321 ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttgggttatc gttttacgat
    25381 agccgcacca cgtcgttgtt gcatcctgct cgccagatcc agcttcccag cctgcaacgc
    25441 ggtgaaggtg aagcgatgct taccgccctc acccatctta gccgttcgcc gctggagcag
    25501 aatatcattc agccactggt gttaagtttg ctgcatcttt gccgcaacgt ggtgaatatg
    25561 ccgccgggta attcgcaacc gcgcggcgat tttctctatc acagcatttg taactgggtt
    25621 caggataatt atgcccagcc gctcacccgc gagagcgtgg cgcagttttt taatatcacg
    25681 cccaatcatc tgtcaaaact gtttgctcag catggaacga tgcgttttat cgagtatgtg
    25741 cgttgagtgc gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcaattcat
    25801 gaagtggcac agcgttgcgg ttttccggat agcgactatt tttgtcgcgt tttccggcgt
    25861 cagtttggtc tgacgccggg agagtacagc gcccgttttc agggctaacg tcagaaggtt
    25921 aattctgttt ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa caacgactgg
    25981 cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc
    26041 ccttcgttag cacccagcgt cagcatgatc accagttcga cttcgcccat ttctgactgc
    26101 cagtcaaccg gtttcgccag ccgggcaatg ctgatactgg aatgacggat ccactgagat
    26161 ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc ttcccgctgc
    26221 cagacatctt cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc gaggttgccg
    26281 cacaggaact ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa gatattctcc
    26341 agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt
    26401 tcctgcgcac tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc
    26461 agttgacgaa gctggctttt caccgccgga atacgcgggc tactcatact cagctcatcc
    26521 aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg
    26581 cctacccatt tgccccgctg atgcgcagtg gtaactattt gctgcaacat gcgcaggaac
    26641 gatggcgtaa tcgggttata tagcggcgat acgcgcgggt tattacgatc gaccgcatac
    26701 agatactggg tcatatcgtt ggagccgata ctgaagaaat cgacctcatc gcagaagtgg
    26761 tcgatgatgt agcacaccga cggcacttcc accatgatcc caagcgtaat cgtctctgca
    26821 tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc tttcacccat
    26881 aagatctgat cgaggctgtg aaccatcggg atcatcaact gggcgttgcc gaaactggcg
    26941 gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag caaattccgg gtaaatacgt
    27001 accgcgcgat agccgaggaa tgggttctct tcctggggaa cattcagata agaaatgctt
    27061 ttatcgccgc cgatgtccat cgtgcgaaag ataatcggct tgtcgcccgc cgccagtagc
    27121 acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg atccatatag
    27181 agcatttcgg tacggaacag cccgacacct tccgcgccgt tggcaaacgc gcctggcgct
    27241 tccagagcgg tgccgatatt cgccgcaata tcaatacgct tgttatcacg ggaataggcc
    27301 agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcgacc
    27361 tgataataac cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca
    27421 agcactgcag gttgcccggc ataacgggca atcgcatcca gcggcaagcc actcagtacc
    27481 gggatcgccg aggcacgggc cagaatcagt gtatgcgagg tgcgcccggt tttctccaga
    27541 atcatacccg caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca
    27601 gccaccagaa tggtcggttt tcccagcacc agattgttgc gcggcttcag ttccggccag
    27661 gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa
    27721 tcgctggcag aagcagatag tttggcgcaa acctgctcca tattgctgat gatcgccgcc
    27781 cccagtccct gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc
    27841 tgaatcagcg acaaatgagc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat
    27901 tgctgattca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc
    27961 gcactggcgg ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta
    28021 cccacgccga cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt
    28081 aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg tcggctgaac gctgtcgctg
    28141 tcgataaagc gcaccaggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg
    28201 ccgctaatgt tcaggctgca actgtcatta aataaggtgc cagttccaat caacgccagc
    28261 gagcttttag catcggcctt tgcgttctgg cgatggttga taaaggtgat ttcgctttgc
    28321 cactggctgc actgttcttt aagttcccgc gccagacgag catgtagacc gttaggcata
    28381 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
    28441 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag ggcagataaa
    28501 agttgctcca tctgcaaaat gtcgcggcaa tcggcaatcg atgccgcgca atgtccgtgg
    28561 cgggtggcag gccccattac caatgtgggt acgccagtac cggttaagtg caccgctcca
    28621 ccgtccgtgc cgccgttact gaacatatcc gcctgcaacg gaacgccaat ttctgccgcc
    28681 acggtttcaa tccacgcggt aagttttggc ggcgcaatca gcgatttatc gcttaacacc
    28741 agcatcggcc cgttaccaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc
    28801 cagcaggcgg tatcaagcac aatggcgacg tccggcgaca ccgcgcgggt agcagtttgc
    28861 ccgccgcgta atcccacctc ttcgctggaa cttgccacca gccacacttc cgcaggcagt
    28921 tcggcgtcgt gcagctcacg tagtaacgtc accagcagat agcaaccgag gcgatcatca
    28981 aaggctttgc ccatcacccg ctggtgaggg aggacctgaa aagtggtatc gaaggtgacg
    29041 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat aggtgcgcgc gccaatgtcc
    29101 acgcgcatgc cgctaacgtc attcccctgc cggtcgccgt caagcaggcc tagaattttg
    29161 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg
    29221 ccaaccggca gcacgtcaat agcgccttcg cgggagatgc tgcgcaccat aaatcccact
    29281 tcgtccatat gggcgcagat catcaccttc ggacctgtcg attcattgag gcggatcagc
    29341 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gagccgcttc ttccagcagg
    29401 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
    29461 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
    29521 cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgcccta
    29581 gcaaataaat ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc
    29641 gcggtgaaaa acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac
    29701 cgaaatagtc gccaaatcca gactcggtaa tgacccggcg cgcggcgtcg tcaatctgct
    29761 ggcagcgcac gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct
    29821 gatagacgtc aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
    29881 tgcgcgtcat atcagcagta gccctgatac agcgcaccaa aatcgagagt gacaaactcg
    29941 cccgctgcaa caatcttgtc gctggctttg ccgtgcggcg gcgccccgcg ccagccgctg
    30001 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
    30061 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
    30121 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
    30181 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
    30241 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
    30301 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
    30361 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
    30421 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
    30481 ttctgccgtg aggagagaag caccgcatcc agttgttgca ccttaagcca gttgcgcagc
    30541 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt
    30601 tgcggaacat catcagacgc aggaacacca ccatcagcgc agtaatgatt gcccccagcg
    30661 cgatccccgc catataaacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
    30721 ccgggaacca ttgcactgcg cccagccaga cagctgcggt tgagccgaca atcgcgccga
    30781 ccatatatga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
    30841 taatccccat aaaggccagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
    30901 acaggcgttt gccggttaaa cgacggtcaa taatggtcgc cagacccaga ccaatcggcg
    30961 gaataacgat agcgatagaa cgagcggtga ccggtaatac gtggtcagtg gtaaagctga
    31021 aggcaacgaa acctgcggct ttgttgatcg ggccaccaag gtcgattgct gtcgccgcgg
    31081 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acagtacgga
    31141 taccgccgtt gatccagcca ccaaaggggg tgatgacgta gtacatcgcc agcatgacaa
    31201 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
    31261 actggatttt ctggttcatc catttcacaa ggtaaccggc aacgatagaa ataatcagcg
    31321 caccaatgaa ggtggacggc aacgatagaa ataatcagcg caccaatgaa ggtggacggc
    31381 accggcgaag aggtcgccca ttgcatagtg ctgggctcga agttcagcag ttgcgtcggc
    31441 tgggtagaca tcaacccgcc gataaaacca gccgggaacg ccagtttgcc accgatagag
    31501 ttcgccacaa atgcagcaaa catcggaatg gcaaaaccaa acaacacgcc gccgaaggac
    31561 tgcgacagcc aggcaaattt aagtaaggag aggtcgaaac cggagaattt cccgctatta
    31621 agagcatcca tgatgccgat ctcagcagga attttaagcc agctataagc aatcagctgg
    31681 ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga aatccctgac
    31741 atcacatgct gcgggagttc accccagaag ctgcttttag aggcctgcgg attcttaact
    31801 gccgctgccg caccggatgc gccaggcaca acggttgcac tgcgtttttt aatggccata
    31861 acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc gatttctttg
    31921 atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga tttcataaac gtcgcgtgat
    31981 tcgaaacgtt cgttatcttc cggggtaact gccacggaat ggatgatgat ggtcgcttcg
    32041 gcgatatcct gcgccgtcag gcgattctgt atgccgtccg cgccctgggt ttcaattttc
    32101 acttcataac cggcttctac cgccgcttct tccagcgcct gagcggccat aaaagtgtga
    32161 gccaggccca ttgggcaggc acataaggca atcagtttct tactcatcgt taattcctcg
    32221 ttcagtgata gttgacgagt gaaattatga acgtgcaaaa caaatcgccg ttaccagaca
    32281 aataatgcat ttactggaga attaatcccg ctgtacggaa gtgtgacgca ggtcggcctt
    32341 gtgaggggac acaaagaaaa attgaattaa ctgtatgatt taaaagatta tcgggagagt
    32401 tacctcccga tataacagga aggatttaca gaatgtgacc taaggtctgg cgtaaatgtg
    32461 caccggaacc gagaagaccc ggatagtcat ggacgatgag atacaccgga atatcatgga
    32521 catattcttt aaagcgccct ttatcttcaa atgcggcacg gaaaccggag gctttgaaga
    32581 actcaaggaa gcgcggcacg ataccgcccg caataaacac gccgccaaat gtcccgagat
    32641 tgagcgccag attgccgcca aaacggccca taatgacgca aaacagcgac aatgcgcggc
    32701 ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat atcttttggc ttgagatttt
    32761 ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa attcaccagc ccagggccag
    32821 aaagcacgcg ctccgccgaa acatgaccaa tttccgcacg caatatttcg aggataatgg
    32881 cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc
    32941 aacgcttatc gacatggacc agatgcgcaa ccccaagccc cgttccggca ccgtaaaccg
    33001 caataggctt accttcgacc ggttctgcgc caccaaactg aatcagatgc tcttttttca
    33061 gcatcgggat cgccatcgat acagcggtaa aatcgttaat aatttccaga tggctaaaac
    33121 cgagattctt tttcatttcg gcaattgaga acgcccaggt atggttggtc attgccaccc
    33181 agtcaccggt aattgggcaa gcgatggcaa tacagccgtc tttcacctcg accttatgtt
    33241 cttcaagata aacgcgaatg accgcttcga ggctggggta atcaagccct gaataggtct
    33301 tagcctgcga gatttcacca ctggcaatat cacacagagc aagacgtgcg ttggtgccgc
    33361 ccacatcacc gactaatgca tactttgtca ttcttcaact gctccgctaa agtcaaaata
    33421 attctttctc acactgtaaa tacttggggg cataacaaca acgccggaaa ggcaggctcc
    33481 ctgtaaatat cgatctgggt cacacaatta ctttatcgtt tcagcaccaa ttgcagcgat
    33541 gcctttttgc aagctgggca aactaagtat ctgaccccgc ataaggaata gaacatgctc
    33601 catccgcgag ccagaaccat gttgttatta tcgctccccg ccgtggcaat tgggattgcg
    33661 tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg
    33721 caacgactgc cgggaactct ggggatagcc caggattcac ccctctggat catcggtgta
    33781 ttaacgctaa cgggtattgc ggtggggttg gttatccgtt tcagccaggg tcatgccgga
    33841 ccagaccccg cctgtgaacc gctgatcggc gcaccggttc cgccctctgc gctacctgga
    33901 cttatcgtag cattaattct cggtcttgct ggcggcgtca gcctggggcc ggaacatccg
    33961 atcatgaccg tcaatatcgc ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc
    34021 aaccgaatgg agtggactat tttagcctct gccggaacca tcggcgcgct gtttggtaca
    34081 cctgttgcgg cggcgttgat attttcgcaa accttaaatg gcagtagtga agttccgcta
    34141 tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg gtgcacttac taccggatta
    34201 tttttccatc cccatttttc actgcccatt gcgcattacg gacagatgga aatgaccgat
    34261 attctcagcg gtgcaattgt cgcggcgatt gccatcgcag cagggatggt cgccgtatgg
    34321 tgcttaccac ggttgcacgc gataatgaat caaatgaaaa atccggtgct cgtgctgggt
    34381 attggcggat ttattctcgg aattctgggg gttattggtg gaccagtttc gctgtttaaa
    34441 gggctggatg agatgcagca gatggtggca aatcaggctt tcagcaccag cgattacttt
    34501 ttgctggcgg taattaaact tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt
    34561 gggcgaatct tcccggcagt gtttgtcggc gtggcattag ggttgatgct gcatgagcac
    34621 gttcccgccg ttccagcggc aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg
    34681 gtaacacgcg atggctggtt aagtcttttt atggcggcag tcgttgtacc caataccaca
    34741 ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc tgttattagc aggtaagccg
    34801 atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg ataatgttta gcaaaaaatg
    34861 ctctccttta tgatttaaga gttatggcga tgatacgggg ctttacctcc ccgtaatatt
    34921 gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt caggtcactg
    34981 tttctggcgg ccgccctgat ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc
    35041 ctactgccat cgatcaaatt acaaattggc gatcgcgatc attacggtaa ttactgggac
    35101 ggtggtcact ggcgagatcg tgattactgg catcgcaatt atgaatggcg caaaaaccgc
    35161 tggtggcgtc atgataatgg ctatcatcgt ggctgggata agcgtaaagc atatgagcgt
    35221 ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg
    35281 caccgccatt aaacgcgtcc tccaatggag cacaatgcct gatgcgacgc tagcgcgtct
    35341 tatcaggcct acaaaccggc tctttaacgc gtagggcgga taaggcgttt acgccgcatc
    35401 cgccaatagt gccagatgcg acgctcattc aactacaatc ccagcgccgt ccccaccaac
    35461 aaccagatat tcagcgccac gaccagcact acaatcaccc agcctgtctg ttttacgcgt
    35521 ttgctgttca ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag cagtggaacc
    35581 agcgccaggg cgataccaaa acttaacagc acctgactca taaccagaat ccgtgtcgga
    35641 tctaatccca tcagaatgac aataaatgac ggcaacatgg tgactgtacg acgcacccac
    35701 agcgggatat ggaagcgaat gaatccctgc atcaccacct gccccgccag tgtccccacc
    35761 accgttgagg acagtccggc agcaaccaga cttaacccaa agaccgttgc cgcagcatgg
    35821 cttaacagcg gttgcagcgt cagataagcc tcatcaagat cggcaacacc agtatgacca
    35881 gaaaagtgga acgccgccgc agctgtagcc atcatcgcca gattgacaaa accggcaatc
    35941 gtcatggcga tagccacatc ccatttggtg gcggaataac gttgttgacg cgaaccgcca
    36001 tgtaaatgct gagtgagcga agagtgcaaa taaatcacat gcggcataat cgtcgcccct
    36061 aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta aactcgggat caccattcct
    36121 ttacccagct gcgccaggtt aggctgggag aaaatcaact cgacaatgta agccgcggca
    36181 acaaacaaca gtaacccgcc aatcactttc tccagcggtt tttgcccgcg acgttgcagc
    36241 attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa cgaaacacca
    36301 agaatgagtt taaaaccgat cgccgcacca ataaattccg ccaggtcggt tgccatcgca
    36361 ataatttctg cctgaaccca atagaaccac actacgggac gcggatagtg atcacgaatc
    36421 tgctccgcca gatttttacc ggtggcaatc cctagtttgg cagagaggat ctgaattagc
    36481 atcgccatca ggttggccca aacgacaacc cacagtagct gatagccgaa gctagcaccc
    36541 gcctgaatat tggtcgcaaa gttaccggga tcgatataac caatcgccgc aatgaacgca
    36601 ggtcccatta atgcgagcct catcttgcgc gccgcccgtc cgctgctact ctcaacgcga
    36661 tagttcgtca tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc tatgctaaac
    36721 gagaatgatt atcaaattca tttaaatgga ttgtggtgat ttctctgata gaccaagatt
    36781 atgattacga aaagattgat gacttgaatg ttatggttat gtttaatgtt agcacattta
    36841 cataactttc agcttccata cacaacatag cagaaatgta tgacagatca ctatttttga
    36901 agcctgtcac aggacgtcat tatagtgtgt gtcagatctc gttttcctta accatgttac
    36961 atagaatgtg cacggaaatt taacctgcct catatttgga gcaaatatgg accgcgtcct
    37021 tcattttgta ctggcacttg ccgttgttgc gattctcgca ctgctggtaa gcagcgaccg
    37081 caaaaaaatt cgtatccgtt atgttattca actgcttgtt atcgaagtgt tactggcgtg
    37141 gttcttcctg aactccgacg ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga
    37201 aaaactactc ggatttgcca acgaagggac taacttcgtc tttggtagca tgaatgatca
    37261 aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct ctgcactgat
    37321 cggtattctc cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct
    37381 ctccaaagtc aacggtatgg gcaaactgga atcctttaac gccgtcagct ccctgattct
    37441 gggtcagtca gaaaacttta ttgcctataa agatatcctc ggcaaaatct cccgtaatcg
    37501 tatgtacacc atggctgcca cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata
    37561 catgaccatg ctggaaccga aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac
    37621 ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat gccagtgaag aaaacatcca
    37681 gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat acattctggc
    37741 aggtttcaaa gttgccatta tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc
    37801 cgcgctgaac gcactgtttg ccaccgttac tggctggttt ggctacagca tctccttcca
    37861 gggcatcctg ggctacatct tctatccgat tgcatgggtg atgggtgttc cttccagtga
    37921 agcactgcaa gtgggcagta tcatggcgac caaactggtt tccaacgagt tcgttgcgat
    37981 gatggatctg cagaaaattg cttccacgct ctctccgcgt gctgaaggca tcatctctgt
    38041 gttcctggtt tccttcgcta acttctcttc aatcgggatt atcgcaggtg cagttaaagg
    38101 cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt ctgaaactgg tttacggctc
    38161 taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaaa accatacata
    38221 aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt aatcagctaa tggctgcggg
    38281 cgaccaatca aatacccttg cagatattgc accccgagct tatgcaatag cgcctgctgc
    38341 tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc cagatcggta
    38401 attgatctca caatcatcgc atccagcgta ttcgtgacaa tatctttcac aaagacgcca
    38461 tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc atatccggtg
    38521 ccaaagtcat caatcgcaat ccggaaacca aacttatgca gctgctcgat gttgtacatg
    38581 ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct caagaatgac cgcctgcggg
    38641 gagatgtgat aacgcttaaa cagacgaata atccgcccgg caatattctt ttgcagcagc
    38701 gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc
    38761 agccacttca acaaggattc cagcacttgc aaatcaaaac gcgcgctaag gttaaactga
    38821 gcaataaggg gcagaaactt atccggggtc ataatgccgc cgtcatattt cagtcgcgcg
    38881 aggatctcat cataaccttc accctctttg ttgcgaattg gctgggcgta gagcagcaaa
    38941 tcaccctgat ctaacgccgt gcgaatggta ttcagcaata gcacctgttt agtggtctgc
    39001 ccggaaacca tctcctctct gctatccagc gccagcacat gatgatgtgc gcaggattgc
    39061 tccgccagcc agcttaactg ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa
    39121 cgcccccagg cagcaccata gcccatatcc agcccggtat tgttccagtg aatttgccga
    39181 ctattcagga tgttaaccat atgctggagt cgcccttccg tttccggccc gctcagcact
    39241 aacagcagtt cactacccgg caattgatac aacttttcgt tttcctgcat caacggcagc
    39301 agcgtacggc aaattgagcg gatacagtga acgcgcatca ttaagccgta atgacgactc
    39361 ataaactcaa gattatcaat gcgcaggcag caaaaactct tgcccgcctc ttgctccggc
    39421 gcttgttcca acgcacgaaa gttgggtagg agcgttaacg gatctgttag cgcctgcaaa
    39481 tgccagcggc gattaagcca ttcactgcga tgataaatgc gcaccatata gagcaggcaa
    39541 acgctaaagg aaatcagcac cgcgagaata aatgccagcg aatattcggt ttccacccct
    39601 tgcaaaaagt tctggttgta attcagaagg caaagcgttg aaacagccta ggtgagattt
    39661 aaaaacggat agcgaagatt accgaccccg agggtaaaga tgatgaagaa aacgggtacc
    39721 aggtaaccgg caataaagtc gttttcataa ggtgtgcaca acagaagtag cagcacgctt
    39781 aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt tgcccaacga cggagcgata
    39841 tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa tcatgcgagt gagatagtag
    39901 aaaagcatgt tgtaaatcag cactgcggtg aaaaggctta gtaaatcaac gaccgtgaaa
    39961 atggcatccg catcgccgaa aaaggtggat atctttagcg ggaaatcaaa gaagctcccc
    40021 acaagataca tgctgcattt gatgccgatc ggcgtcacca aaccaagcca gaacagacgt
    40081 tgccagacat atcggctggt caatccataa cgccaacgcg tccccagctg ccagcgtaat
    40141 attgcgcagg cacacaacac gacaaacgtc tggctgccga gcatgaccgc agtctgtaaa
    40201 aaagagagat gaaagttcca cagattggtg caaaacatcc cgcataaaac gggaacgaca
    40261 ccgcgccagc caaagataaa caagattgac agcattacgc acagtggcat ccacgccaga
    40321 aagatataac tggaatggat aatggcaagt ggcgaaataa aacgggatag ctgaataagt
    40381 accacggtga gcgtaaacgc tagtgtgaat atcttgatat tttttatcag gttatgctcc
    40441 acaaacatga gtacactact ctcactgatc ggggttttca ggcacacaaa taataagttt
    40501 attcgcatcg cgaagcaaac aggggccagc ctgttgccga tttttcatcc aatgatgctt
    40561 tcaccaggcg gcgctaaccc ggacgtaaac tgtattacaa aagcggcaaa aagcagagac
    40621 aaaaaacccc cgctttgcag cgagggttg
//
LOCUS       DADMFL010000022        40347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.022, whole genome shotgun sequence.
ACCESSION   DADMFL010000022 DADMFL010000000
VERSION     DADMFL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.022"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            235..579
                     /locus_tag="JNZ27_001183"
     CDS             235..579
                     /locus_tag="JNZ27_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8663592.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            602..973
                     /locus_tag="JNZ27_001184"
     CDS             602..973
                     /locus_tag="JNZ27_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8663593.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(1025..2440)
                     /gene="gltX"
                     /locus_tag="JNZ27_001185"
     CDS             complement(1025..2440)
                     /gene="gltX"
                     /locus_tag="JNZ27_001185"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY8663594.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            2699..2774
                     /locus_tag="JNZ27_001186"
     tRNA            2699..2774
                     /locus_tag="JNZ27_001186"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2732..2734,aa:Val,seq:tac)
     gene            2819..2894
                     /locus_tag="JNZ27_001187"
     tRNA            2819..2894
                     /locus_tag="JNZ27_001187"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Val,seq:tac)
     gene            2899..2974
                     /locus_tag="JNZ27_001188"
     tRNA            2899..2974
                     /locus_tag="JNZ27_001188"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2932..2934,aa:Lys,seq:ttt)
     gene            3005..3130
                     /locus_tag="JNZ27_001189"
     ncRNA           3005..3130
                     /ncRNA_class="other"
                     /locus_tag="JNZ27_001189"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            3250..3591
                     /locus_tag="JNZ27_001190"
     CDS             3250..3591
                     /locus_tag="JNZ27_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY8663595.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(3582..4508)
                     /locus_tag="JNZ27_001191"
     CDS             complement(3582..4508)
                     /locus_tag="JNZ27_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8663596.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4598..5596
                     /locus_tag="JNZ27_001192"
     CDS             4598..5596
                     /locus_tag="JNZ27_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY8663597.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMTRRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5593..5811)
                     /locus_tag="JNZ27_001193"
     CDS             complement(5593..5811)
                     /locus_tag="JNZ27_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663598.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5813..7828)
                     /gene="ligA"
                     /locus_tag="JNZ27_001194"
     CDS             complement(5813..7828)
                     /gene="ligA"
                     /locus_tag="JNZ27_001194"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY8663599.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7899..8786)
                     /gene="zipA"
                     /locus_tag="JNZ27_001195"
     CDS             complement(7899..8786)
                     /gene="zipA"
                     /locus_tag="JNZ27_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY8663600.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            9016..9777
                     /gene="cysZ"
                     /locus_tag="JNZ27_001196"
     CDS             9016..9777
                     /gene="cysZ"
                     /locus_tag="JNZ27_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY8663601.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGTTPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9962..10933
                     /gene="cysK"
                     /locus_tag="JNZ27_001197"
     CDS             9962..10933
                     /gene="cysK"
                     /locus_tag="JNZ27_001197"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY8663602.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11317..11574
                     /gene="ptsH"
                     /locus_tag="JNZ27_001198"
     CDS             11317..11574
                     /gene="ptsH"
                     /locus_tag="JNZ27_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY8663603.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11619..13346
                     /gene="ptsI"
                     /locus_tag="JNZ27_001199"
     CDS             11619..13346
                     /gene="ptsI"
                     /locus_tag="JNZ27_001199"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY8663604.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13387..13896
                     /gene="crr"
                     /locus_tag="JNZ27_001200"
     CDS             13387..13896
                     /gene="crr"
                     /locus_tag="JNZ27_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY8663605.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13938..14789)
                     /gene="pdxK"
                     /locus_tag="JNZ27_001201"
                     /gene_synonym="thiJ"
     CDS             complement(13938..14789)
                     /gene="pdxK"
                     /locus_tag="JNZ27_001201"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY8663606.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14894..15268
                     /locus_tag="JNZ27_001202"
     CDS             14894..15268
                     /locus_tag="JNZ27_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY8663607.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            15301..16072
                     /locus_tag="JNZ27_001203"
                     /pseudo
     CDS             15301..16072
                     /locus_tag="JNZ27_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16228..17139)
                     /gene="cysM"
                     /locus_tag="JNZ27_001204"
     CDS             complement(16228..17139)
                     /gene="cysM"
                     /locus_tag="JNZ27_001204"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY8663608.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17273..18370)
                     /gene="cysA"
                     /locus_tag="JNZ27_001205"
     CDS             complement(17273..18370)
                     /gene="cysA"
                     /locus_tag="JNZ27_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY8663609.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTCLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18360..19235)
                     /gene="cysW"
                     /locus_tag="JNZ27_001206"
     CDS             complement(18360..19235)
                     /gene="cysW"
                     /locus_tag="JNZ27_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY8663610.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19235..20068)
                     /gene="cysT"
                     /locus_tag="JNZ27_001207"
     CDS             complement(19235..20068)
                     /gene="cysT"
                     /locus_tag="JNZ27_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY8663611.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20068..21084)
                     /locus_tag="JNZ27_001208"
     CDS             complement(20068..21084)
                     /locus_tag="JNZ27_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8663612.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(21388..22179)
                     /gene="ucpA"
                     /locus_tag="JNZ27_001209"
     CDS             complement(21388..22179)
                     /gene="ucpA"
                     /locus_tag="JNZ27_001209"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY8663613.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22366..23367)
                     /gene="murR"
                     /locus_tag="JNZ27_001210"
     CDS             complement(22366..23367)
                     /gene="murR"
                     /locus_tag="JNZ27_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY8663614.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            23330..24226
                     /gene="murQ"
                     /locus_tag="JNZ27_001211"
     CDS             23330..24226
                     /gene="murQ"
                     /locus_tag="JNZ27_001211"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY8663615.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPDLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24230..25654
                     /gene="murP"
                     /locus_tag="JNZ27_001212"
     CDS             24230..25654
                     /gene="murP"
                     /locus_tag="JNZ27_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY8663616.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(25834..26733)
                     /gene="yfeX"
                     /locus_tag="JNZ27_001213"
     CDS             complement(25834..26733)
                     /gene="yfeX"
                     /locus_tag="JNZ27_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY8663617.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(26829..27404)
                     /locus_tag="JNZ27_001214"
     CDS             complement(26829..27404)
                     /locus_tag="JNZ27_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY8663618.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27465..27914)
                     /locus_tag="JNZ27_001215"
     CDS             complement(27465..27914)
                     /locus_tag="JNZ27_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY8663619.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(27901..28326)
                     /locus_tag="JNZ27_001216"
     CDS             complement(27901..28326)
                     /locus_tag="JNZ27_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY8663620.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28540..29409
                     /gene="amiA"
                     /locus_tag="JNZ27_001217"
     CDS             28540..29409
                     /gene="amiA"
                     /locus_tag="JNZ27_001217"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY8663621.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29413..30312
                     /gene="hemF"
                     /locus_tag="JNZ27_001218"
     CDS             29413..30312
                     /gene="hemF"
                     /locus_tag="JNZ27_001218"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY8663622.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30318..31370)
                     /gene="eutR"
                     /locus_tag="JNZ27_001219"
     CDS             complement(30318..31370)
                     /gene="eutR"
                     /locus_tag="JNZ27_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY8663623.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31455..31916)
                     /gene="eutK"
                     /locus_tag="JNZ27_001220"
     CDS             complement(31455..31916)
                     /gene="eutK"
                     /locus_tag="JNZ27_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY8663624.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(31929..32588)
                     /gene="eutL"
                     /locus_tag="JNZ27_001221"
     CDS             complement(31929..32588)
                     /gene="eutL"
                     /locus_tag="JNZ27_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY8663625.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32598..33485)
                     /locus_tag="JNZ27_001222"
     CDS             complement(32598..33485)
                     /locus_tag="JNZ27_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY8663626.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPG
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33506..34867)
                     /locus_tag="JNZ27_001223"
     CDS             complement(33506..34867)
                     /locus_tag="JNZ27_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY8663627.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            35056..36261
                     /locus_tag="JNZ27_001224"
     CDS             35056..36261
                     /locus_tag="JNZ27_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8663628.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            36781..37545
                     /locus_tag="JNZ27_001225"
     CDS             36781..37545
                     /locus_tag="JNZ27_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8663629.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAE
                     EDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLY
                     TWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGK
                     CGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            38596..39348
                     /locus_tag="JNZ27_001226"
     CDS             38596..39348
                     /locus_tag="JNZ27_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663630.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            40108..>40347
                     /locus_tag="JNZ27_001227"
     CDS             40108..>40347
                     /locus_tag="JNZ27_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8663631.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEV"
BASE COUNT        10031 a        10729 c        10018 g         9569 t
ORIGIN      
        1 tcacgtaaag cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa
       61 cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga
      121 aaaccgctcg cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa
      181 tgaactacta atagacccac atacattcag ggaattgtta tgtccaagga gaggatgaca
      241 ccagatgaac ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc
      301 aaggaaggct ggacaacgtc accaaaacct ggcgtccagg gtggcaaagc ccgactggtt
      361 cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg aacgtccaga aggtcaggga
      421 gaagcgcctg ccctttctgg tgatgcgccg cttgaagtat tgctggtgac actggcgaaa
      481 gagatgacgc cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc
      541 ggattgttac aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa
      601 aatgaccacc gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg
      661 gatcagagaa aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag
      721 acttattctg gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa
      781 cacaccaatg ttgatggaag ctgaagagag aattgctgaa tacgctcctg gagcacgcgc
      841 cccggcgtat cgacaaatca tcaacgcgat agataatatg acagacattg agcaggaaaa
      901 agtcgcgcaa tttttgtcac gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga
      961 cgaatcagca taaataaaaa acggcaggat attatctcct gccgtttatc tttttacacg
     1021 ctaattactg ctgattttcg cgttcagcaa taaaatccag cgctttgttg atacgctcga
     1081 tactgcgggt cttaccaatt gcgtgaacgg taacatccag tgctggagac tgccccgcac
     1141 cggttacggc gacacgcagc ggcataccaa ctttacccat acccacttcc agctcatccg
     1201 ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt
     1261 tgtcacgaac cacttccagc ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat
     1321 cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca gctctgtgcc atctctttca
     1381 gcgtcttgca gcgttcgccc agcagtttca ccagatcagc cagctgcggg ccgttacggg
     1441 tatcgatatt ttcctgctca atgtgccact gtaagtgagt agcaacatac tccggcggca
     1501 gcgcgttaat gtagtgatgg ttcagccaca gcagcttgtc ggtgttgaac gcactggcag
     1561 atttgctgac ggcattcaaa gtgaagtatt tgatcatctc ttcacgagtg aagatttcct
     1621 gatcgccgtg ggaccagccc agacgcacca gatagttcag tagtgcttct ggcaaataac
     1681 cgtcatcacg atactgcatt acgctgactg ccccgtgacg tttggacagt tttttaccgt
     1741 catcgccatt gatcatagaa acgtgcgcgt aaaccggcac cggcgctttc agggccttaa
     1801 gaatgttgat ctggcgcggc gtgttgttga tatggtcttc gccacggata acgtgggtga
     1861 tttccatatc ccagtcatcg acaaccacac agaagttata ggttggggaa ccatcggtac
     1921 ggcggataat aagatcgtcc agttcctggt tgctgaactc gatcggacca cggatctgat
     1981 cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg tacaacacac ggttcatcat
     2041 cagcatgatg ctcatggctg tggcggcagc gaccgtcata acgcggcttc tcacctttcg
     2101 ccatttgctc ttcgcgcagc gcttccaggc gctctttaga gcaatagcat ttataagcag
     2161 tgccctcttc cagcatctga tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt
     2221 agtacggacc ttcatcccac tccaggctca gccagttcat gccatccata atggcttcga
     2281 tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc
     2341 cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg cgcgccgcca acgtgcagat
     2401 agcctgttgg gcttggcgcg aagcgagttt tgattttcat gaaatggcct tacgtttaga
     2461 aagatgccga caaccggcaa atcctggaaa aattgagtgg gcgatattct atcactcccg
     2521 cctgattcct caatgtagtt cgggtttcta ccttacgcat tattgttttc gttgacaaat
     2581 tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt tacgacgaac gaacaatttc
     2641 tttagaaaat gcgttgactc attttgaact ctccctataa tgcgactcca cacagcgggg
     2701 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     2761 gtcatcaccc accaactact ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg
     2821 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     2881 gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
     2941 ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct
     3001 tttttactat ctgcgatact caaagattcg aacctgcagc aggtttgagt tgagcgcagc
     3061 gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca
     3121 cgacccacca atgcaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag
     3181 attgattttt cttctcaagt tccttgttct tctaccgata ttacctcgta cagtgacgag
     3241 gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt
     3301 aacagggcat cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc
     3361 atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc tgaagacaag
     3421 caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag
     3481 ttgcagaaac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc tctgaatgta
     3541 tcccttctaa atcctgttga aaaaacgact catatcgata tctatatctg atacaacgga
     3601 tccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
     3661 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaacc
     3721 accggcgtaa tgcgcttaac caccagtggg ctaccttcag gcaatggtag cgccagtgca
     3781 ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
     3841 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
     3901 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
     3961 acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa
     4021 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
     4081 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcatg caggacaaac
     4141 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     4201 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     4261 tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc
     4321 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     4381 ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat acgctctcct
     4441 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
     4501 taattcatct gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga
     4561 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
     4621 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
     4681 cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat
     4741 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca
     4801 tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg
     4861 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
     4921 cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc
     4981 ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcgc cattgctggt
     5041 tggtctggtg atgaacgttc acggtgcaag gggcagcctt gagcaggtcg gtaaaattat
     5101 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
     5161 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
     5221 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
     5281 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt
     5341 ctttatgaca cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg
     5401 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt
     5461 gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc
     5521 ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag
     5581 cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt
     5641 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt
     5701 gcgggcaaac cacagcagat actcctccgg caggtcaatt aagcgacgcc ctttgtattt
     5761 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
     5821 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg
     5881 ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc
     5941 ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc
     6001 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
     6061 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
     6121 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
     6181 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
     6241 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
     6301 gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccaattttg
     6361 gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag
     6421 tatggacata ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat
     6481 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca
     6541 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
     6601 ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga
     6661 cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
     6721 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
     6781 cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
     6841 gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgctaccg
     6901 cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct
     6961 taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat
     7021 agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc
     7081 cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
     7141 gaacaccaac gccatagcag aaaaaagtga gcggccgctt cgctgtaata cgcggatcaa
     7201 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc
     7261 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca
     7321 cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg cgaatagtac
     7381 gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta
     7441 aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc
     7501 aagtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc
     7561 tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga
     7621 aagctgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg
     7681 gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa
     7741 tttccggcgc atccatcaca tgataaagat attcatgatg gcaaagcgtc gttcgcagtt
     7801 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
     7861 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact
     7921 tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg
     7981 tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
     8041 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta
     8101 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat
     8161 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
     8221 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
     8281 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
     8341 gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc
     8401 tgttgtgccg gttgcggtgc tgaatgcact ggctgcggcg ctggcgcgac ctgtggcgaa
     8461 actggctgct ggaccggttg acgcggctgc gcagacgcat aaggcggttg gtactggtgt
     8521 tgcggcgacg gacgagcagc ctcatgctcc tgagcgttag ccggggcatg attcacgcgg
     8581 tgaacacgaa cctcaccaac gccctcatca tcttcgacat cctcgtcata agaatcgtcg
     8641 tcacgttttg acttcattcg ttttaatggc cgatcgcgga acatagaaga tcgttcttta
     8701 cggctggtcc agaaaccatg taccagtaaa gcgattatgg cgatcgcgcc aacaatgatt
     8761 aatatcagac gcaaatcctg catcattata ttctctgttg ttctaacacc ttgccaccac
     8821 ggcaaacatt tactcactaa gagtatttgc cgattacctc aagtgcaagt gcactattaa
     8881 ctttcacagc acaaagatag atgaaatcgt gctttttgct gttttttcga acatatccta
     8941 actgtccatt gcgcaattac ccggtaaaat acgcagaatt ttcctgggat tggtcaaaag
     9001 gagctcatcc tgattatggt ttcatcattc acatctgccc cacgcagcgg tttttactat
     9061 tttgcgcaag gctggaagct cgtctcgcaa cctgggattc ggcgtttcgt tattttaccg
     9121 ctgctggtca atattttgtt gatggggggc gcattctggt ggctctttac acagctcgat
     9181 gtctggatcc cgactttcat gagttacgtt ccggactggc tacaatggct gagttatctg
     9241 ttgtggcctc tggctgtcat ctctgtgctg ttagtgtttg gctatttctt ctccacgatt
     9301 gctaactgga ttgccgctcc gtttaacggt ttattggctg aacaactgga agcacgattg
     9361 actggcacta caccgccaga taccgggatt ttcggtatca tgaaagatgt gccgcgaatc
     9421 atgaaacgcg aatggcaaaa atttgtctgg tatctgccgc gcgcaattgt attactaatt
     9481 ctttacttca tccctggtat tgggcaaacc gtcgcgccgg tactgtggtt cctgtttagc
     9541 gcctggatgt tagccatcca gtattgcgat taccccttcg ataaccacaa agtgccgttt
     9601 aaagagatgc gcaccgccct gcgcacacgc aaaatcacca atatgcagtt tggtgcttta
     9661 accagcctgt ttacgatgat cccgctgctt aatctgttca tcatgcccgt tgccgtttgt
     9721 ggcgcgacgg cgatgtgggt cgattgctat cgcgataaac acgcgatgtg gcggtaacaa
     9781 tctaccggtt attttgtaaa ccgtttgtgt gaaacagggg tggcttatgc cgccccttat
     9841 tccatcttgc atgtcattat ttcccttctg tatatagata tgctaaatcc ttacttccgc
     9901 atattctctg agcgggtatg ctacctgttg tatcccaatt tcatacagtt aaggacaggc
     9961 catgagtaag atttttgaag ataactcgct gactatcggt cacacgccgc tggttcgcct
    10021 gaatcgcatc ggtaacggac gcattctggc gaaggtggaa tctcgtaacc ccagcttcag
    10081 cgttaagtgc cgtatcggtg ccaacatgat ttgggatgcc gaaaagcgcg gcgtgctgaa
    10141 accaggcgtt gaactggttg aaccgaccag cggtaatacc gggattgcac tggcctatgt
    10201 agctgccgct cgcggttaca aactcaccct gaccatgcca gaaaccatga gtattgaacg
    10261 ccgcaagctg ctgaaagcgt taggtgcaaa cctggtgctg acggaaggtg ctaaaggcat
    10321 gaaaggcgca atccaaaaag cagaagaaat tgtcgccagc aatccagaga aatacctgct
    10381 gctgcaacaa ttcagcaatc cggcaaaccc tgaaattcac gaaaaaacca ccggtccgga
    10441 gatctgggaa gataccgacg gtcaggttga tgtatttatt gctggcgttg ggactggcgg
    10501 tacgctgact ggcgtcagcc gctacattaa aggcaccaaa ggcaagaccg atcttatctc
    10561 tgtcgccgtt gagccaaccg attctccagt tatcgcccag gtgctggcag gtgaagagat
    10621 taaacctggc ccgcataaaa ttcagggtat tggcgctggt tttatcccgg ctaacctcga
    10681 tctcaagctg gtcgataaag tcattggcat caccaatgaa gaagccattt ctaccgcccg
    10741 tcgtctaatg gaagaagaag gtattcttgc aggtatctct tctggagcag ctgttgccgc
    10801 agcgttgaaa ctacaagaag atgaaagctt taccaacaag aatattgtgg ttattctacc
    10861 atcatcgggt gagcgttatt taagcaccgc attgtttgcc gatctcttca ctgagaaaga
    10921 attgcaacag taatgccagc ttgttaaaaa tgcgtaaaaa agcacctttt taggtgcttt
    10981 tttgtggcct gcttcaaact ttcgcccctc ctggcattga ttcagcctgt cggaactggt
    11041 atttaaccag actaattatt ttgatgcgcg aaattaatcg ttacaggaaa agccaaagct
    11101 gaatcgattt tatgatttgg ttcaattctt cctttagcgg cataatgttt aatgacgtac
    11161 gaaacgtcag cggtcaacac ccgccagcaa tggactgtat tgcgctcttc gtgcgtcgcg
    11221 tctgttaaaa actggcgcta acaatacagg ctaaagtcga accgccaggc tagactttag
    11281 ttccacaaca ctaaacctat aagttgggga aatacaatgt tccagcaaga agttaccatt
    11341 accgctccga acggtctgca cacccgccct gctgcccagt ttgtaaaaga agctaagggc
    11401 ttcacttctg aaattactgt gacttccaac ggcaaaagcg ccagcgcgaa aagcctgttt
    11461 aaactgcaga ctctgggcct gactcaaggt accgttgtga ctatctccgc agaaggcgaa
    11521 gacgagcaga aagcggttga acatctggtt aaactgatgg cggaactcga gtaatttccc
    11581 gggttctttt aaaaatcagt cacaagtaag gtagggttat gatttcaggc attttagcat
    11641 ccccgggtat cgctttcggt aaagctctgc ttctgaaaga agacgaaatt gtcattgacc
    11701 ggaaaaaaat ttctgccgac caggttgctc aggaagttga acgttttctg agcggtcgtg
    11761 ccaaggcatc agcccagctg gaaacgatca aaacgaaagc tggtgaaacg ttcggtgaag
    11821 aaaaagaagc catctttgaa gggcatatta tgctgctcga agatgaggag ctggagcagg
    11881 aaatcatagc cctgattaaa gataaacaca tgacagctga cgcagctgct catgaagtta
    11941 tcgaaggtca ggcttctgcc ctggaagagc tggatgatga atacctgaaa gaacgtgcgg
    12001 ctgacgtacg tgatatcggt aagcgcctgc tgcgcaacat cctgggcctg aagattatcg
    12061 acctgagcgc cattcaggat gaagtcattc tggttgccgc tgacctgacg ccgtccgaaa
    12121 ccgcacagct gaacctgaag aaggtgctgg gtttcatcac cgacgcgggt ggccgtactt
    12181 cccacacctc tatcatggcg cgttctctgg aactgcctgc tatcgtgggt accggtagcg
    12241 tcacctctca ggtgaaaaat gacgactatc tgattctgga tgccgtaaat aatcaggttt
    12301 acgtcaatcc aaccaacgaa gttattgata aaatgcgcgc tgttcaggag caagtggctt
    12361 ctgaaaaagc agagcttgct aaactgaaag atctgccagc tattacgctg gacggtcacc
    12421 aggtagaagt atgcgctaac attggtacgg ttcgtgacgt tgaaggtgca gagcgtaacg
    12481 gcgctgaagg cgttggtctg tatcgtactg agttcctgtt catggaccgc gacgcgctgc
    12541 ccactgaaga agaacagttt gctgcttaca aagcagtggc tgaagcgtgt ggctcgcagg
    12601 cggttatcgt tcgtaccatg gacatcggcg gcgacaaaga gctgccatac atgaacttcc
    12661 cgaaagaaga gaacccgttc ctcggctggc gcgctatccg tatcgcgatg gatcgtaaag
    12721 agatcctgcg cgatcagctc cgcgctatcc tgcgtgcctc ggctttcggt aaattgcgca
    12781 ttatgttccc gatgatcatc tctgttgaag aagtgcgtgc actgcgcaaa gagatcgaaa
    12841 tctacaaaca ggaactgcgc gacgaaggta aagcgtttga cgagtcaatt gaaatcggcg
    12901 taatggtgga aacaccggct gccgcaacaa ttgcacgtca tttagccaaa gaagttgatt
    12961 tctttagtat cggcaccaat gatttaacgc agtacactct ggcagttgac cgtggtaatg
    13021 atatgatttc acacctttac cagccaatgt caccgtccgt gctgaacttg atcaagcaag
    13081 ttattgatgc ttctcatgct gaaggcaaat ggactggcat gtgtggtgag cttgctggcg
    13141 atgaacgtgc tacacttctg ttgctgggga tgggtctgga cgaattctct atgagcgcca
    13201 tttctatccc gcgcattaag aagattatcc gtaacacgaa cttcgaagat gcgaaggtgt
    13261 tagcagagca ggctcttgct caaccgacaa cggacgagtt aatgacgctg gttaacaagt
    13321 tcattgaaga aaaaacaatc tgctaatcca cgagatgcgg cccaatttac tgcttaggag
    13381 aagatcatgg gtttgttcga taaactgaaa tctctggttt ccgacgacaa aaaggatacc
    13441 ggaactattg agatcattgc tccgctctct ggcgagatcg tcaatatcga agacgtgccg
    13501 gatgtcgttt ttgcggaaaa aatcgttggt gatggtattg ctatcaaacc aacgggtaac
    13561 aaaatggtcg cgcctgtaga cggtaccatt ggtaaaatct ttgaaaccaa tcacgcgttc
    13621 tctatcgaat ctgatagcgg cgttgaactg ttcgtccact tcggtatcga caccgttgaa
    13681 ctgaaaggcg aaggcttcaa gcgtattgct gaagaaggtc agcgcgtgaa agttggcgat
    13741 actgtcattg aatttgatct gccgctgctg gaagagaaag ccaagtctac cctgactccg
    13801 gttgttatct ccaacatgga cgaaatcaaa gaactgatca aactgtccgg tagcgtaacc
    13861 gtgggtgaaa ccccggttat ccgcatcaag aagtaattct tgccgcagtg aaaaatggcg
    13921 cccatcggcg ccatttttta tgcttccgcc agcggcggca aaatcaattc atcgctctca
    13981 tgctgctggg tgtagcgcat cacttccagt acgcgcaacc ccgcacggtg caccgcatcg
    14041 tttaacgcct tccctttcag caaaccactg ataagctgag cacaaaacag gtcgccagtc
    14101 cctttcaggt cggtttttac ccgtgaatgg gaaatgacat tcacgctgtc ggcactgacc
    14161 accaccacct gcatctcctg attttcttca ttaccggagg cgctggtaat caccacccat
    14221 tttaatgtgt ctgaaagcag actttttgcg gcagcaatgg cactgtcgag atcgcggcaa
    14281 tttttaccgg tcaggatttc cagctcaaag atattggggg taattccctg cgccagcggc
    14341 agtaaatatt gtcgatacgc ctcgggaagg tcaggtttga cataaattcc gctatcaata
    14401 tcgccaatca ccggatcgac catgatcaat aggtcaggat ggtctttgcg tagcgcagtc
    14461 agccactcgg caaggatttt gatttgcgat gccgttccca tatagcccgt ggttacagca
    14521 cgaagttggc gcagcgcatc acgctcctga agcgcacgca aatagccgct aaaccattcg
    14581 tccggaatcg caccaccgta gaaagtgtca taatgcggcg tattgctcag caataccgtc
    14641 ggcacggcaa agacattcaa gccgttctgt tttatagcag gcaccgcaat gctgttgcct
    14701 acgcttccgt acactacctg cgactgcaca gcgacgatat ccgcctgcag cgccctactc
    14761 ttatcgttaa acaacaacaa actactcatt taattttttc tccttgccga tgatcctcat
    14821 cgtattccaa ccgaaacttt acctgattct ggcagtcaaa tcggctatta caaaacaaag
    14881 ataaggtaat tcagtgaaga aaatcatttg tctggccatt acgctgctaa tgacactccc
    14941 cgcttacgcg aagttaactg ttcatgaaga agcccgcatc aacgccatgc tggagggatt
    15001 agcacagaaa aaggatttga tatttgtgcg caacggtgat gaacatacct gcgatgaagc
    15061 ggtttcccac ctgcgtctga agctcggcaa tacccgtaac cgcattgaca ctgccgagca
    15121 gtttattgat aaggtagctt cgtcgtcatc gattaccggg aagccgtata tcgtgaagat
    15181 ccccggtaag agcgatgaga acgcacagcc ttttttacat gcgttaattg cgcagacgga
    15241 taaaacggtg cctgcggaag gaaattaatc cgctttggta aggcatttac aggaggtaac
    15301 atgaaaaaat gctttattta tcacgatgaa aaatcgaata aattttggtg gatagattac
    15361 gaaggggata gtttagctgt caactatggc aaggtaggta gtattggtaa attccataca
    15421 aaagaattcg ataatgaaga acagtgtctg aaagaagcca gtaaattgat tgccgcaaaa
    15481 atgaaaaaag gatatcaaga aactccagac tttaacttca tggatctcta ctattttgat
    15541 gatgaagaga ttgggttaca tcttaaaacg tcacacccaa acttccagtg ccattttact
    15601 gatccacttt atatgtgttg ctgggatgaa gaatctcctt ttggtagcga tgaaggtgct
    15661 gatgctctaa acgaacttga aaatagcctc cgttctggac tgtgctgatt tccctcaaat
    15721 gttaattgaa actatgtggg gtatgaaata catcgctatg gacagtattc ttgaagagga
    15781 tgtgcgtgcg caattactag ccgatgaaat gagctctatc cagagcaata tgattaccta
    15841 cgcgactgca ttcggtcaga tcaaagtcat gggtaaaatc tcccataaac ttaaaaagat
    15901 gggactcaat gcactagcgc gtcatcagct taccgcaaaa attcttcaat ggggtgacgg
    15961 tcaggactca ccaatacttc aaaaaatgat tgatgacctt actgcttttc cacacgaaaa
    16021 taaaaaacta catttgtcgg cagcaacaac tgccgacaaa tcagattatt gacaaagtgc
    16081 actttgttta tgccggatgc ggcgtaaacg cctgatccgg cataaaaata ttgctaattc
    16141 aatatattgc agaaacattg taggcctgat aagcggagcg catcaggcag ttttgcgttt
    16201 gtcatcagtc tccaatgcta ttaatcctta aatccctgcc ccctgactaa aatgctcttc
    16261 cccaaacact ccggtggaga ggtagcgatc gccacgatcg cagatgatcg ctaccaccac
    16321 cgcaccaggg ttagcttttg ccacccgcag tgctccagca accgcgccgc ctgagctgac
    16381 gccacagaat attccttccc gcaccgccag ttcgcgcatg gtgttttccg catcgcgctg
    16441 atgaatatcc agcacctcat ccaccagcgc agcgttgaaa atccccggca gatattccgc
    16501 aggccagcgg cgaatgcccg gaatactgct gccttcttcc ggttgcaggc caacaatggt
    16561 caccggtttg gattgttcgc gcataaagcg tgaaacgccg gtgatagtgc cggtcgtccc
    16621 catgctggag acaaaatgag tgatgcgccc gccggtttgc tgccagattt ccggcccggt
    16681 ggtggtgtaa tgcgcataag ggttatcggg attattgaac tgatcgagca gctttccttc
    16741 gccacgattc gccatctcca gcgccagatc gcgcgcacct tccatgccct gctctttggt
    16801 gacaagaatc agttccgcac cataagcacg catcgccgca cggcgttcct ggctcatgtt
    16861 gtcgggcatc agcaatttca tgcgatagcc tttcagcgcg gcaatcattg ccagcgcaat
    16921 gccggtgtta ccactggtgg cttcgattaa gacatcaccc ggtttaattt ccccgcgctt
    16981 ttccgcctcg acgatcatcg aaagtgccgc acgatctttc accgaacctg ccgggttatt
    17041 gccttccagt tttaaccaca cttcactgcc gttatccggc cccattcgct gcaacttcac
    17101 cagaggcgta ttgcctattg tttgttctaa tgtactcacg atctctatcc atacgtggtg
    17161 ttgcctgatg cgacgcttgc gcgtcttatc aggcctacag gttacaaacc ttgccattaa
    17221 aaagcccgga tcgcgggaag cgcctccggg cgtttaacat tcactcaacc tatcaggcgc
    17281 tttgtgcgag agcaagttcc tcatcgcggg tttcaatacg ctcgtcgccg ttatacagcc
    17341 gcgcatgttg cagaccaacg aataaacgct cgccacgctg cggggcatcg tcgccatgca
    17401 tcacgaccgt cagcggttcg ttgtaccacc ccagcggctg caccactaat tgggtgtagt
    17461 gacctttcgg gctggcttcc agtacctgta ccggcagcgg cgaatcgaga caggtacggc
    17521 ggctgatatc cacttcccac gggcgcagga agagatccac cggaccctga tacgcaggtg
    17581 tgtagcccag cggccagcga tgcgcgccaa catggaactg cccgccgcga atggttccct
    17641 gcaggcggtt cacttcgccc ataaattcga gtacaaaacg ggtcgctggt tcgcgccata
    17701 cctgattcgg cgcatcagcc tgttcaatat tcccctgact catcaccact acacgatcgg
    17761 ctacttcggt cgcctcttcc tggtcgtggg tcacaaacac gctggtgaat ttcagctctt
    17821 catgcagttg acgcagccag cgacgcagct ctttacgcac ctgcgcatcc agcgcgccaa
    17881 acggttcatc aagcagcaga atttgcggtt ctacagccag cgcgcgcgcc agcgccacac
    17941 gctgtttctg gccgccggaa agctgcgccg gataacgatc cgccagatgc gcaagctgga
    18001 ccatttccag caattttgtc actttcgctt tgatggctgc ggcattcggg cgctcgcgac
    18061 gcggcagcac cgtcaggcca aaagtgatat tgtcgaatac cgtcatatgg cggaacagcg
    18121 cgtaatgctg gaacacgaaa ccgactttac gatcgcgcgc gtgcaggcga ctcacgtcgg
    18181 tgccgtggaa gcgaatatgc ccgctggttt gatgctccag cccggcgata atccgcagca
    18241 gcgtggtttt cccggaaccg gacggcccca gcaacgcgac catctgacca gaaggaatat
    18301 ccagtgagat atcgttcagc acctgggtgc gaccaaaaga cttcttgata ttggcaatct
    18361 caatgctcat gatgctcctc ctgctgcgcg cgtttttcct gattctccag gcgccactgc
    18421 aacatactct ttaaaaacag ggtgataatc gccatcagcg tcaacagcgc ggcggcggta
    18481 aaggagccga cggtgttgta gtcctgctcc aacaattcaa tctgtaacgg cagcgaaagt
    18541 gtttcgccgc gaatcgagcc ggaaaccacc gacacagcac caaactcgcc aatcgcgcgg
    18601 gcgttggtca gcaccacgcc gtaaagcagc gcccagcgaa tattcggcaa tgtgacgcga
    18661 cggaacatct gccagccgga cgcgccaagc aaaatcgccg cttcgtcttc ctggctgccc
    18721 tggcttaaca tcaccggcac cagctcgcgc accacaaacg gacacgtcac aaagatggtg
    18781 accagcacca ttccgggcca ggagaacata atttgcagat tatgttcatc cagccagccg
    18841 ccgagcgggc cgttggagcc gtagaacagc agatacacca gaccggcaac caccggcgat
    18901 acggcaaacg gaatgtccag tagcgtcagc agtaactggc gtcccgggaa gttaaagcgc
    18961 gtcaccagcc aggccagcag aatgccgaac accaggttta ccggtacggc aatcagcgcg
    19021 atcatcaccg tcagccagat ggcgtgcagc atgtccggat cggccagatt ctgtaaaacc
    19081 ggcatcagcc ccttgctgaa tgcctgcacg aagatgtaaa tcatcggcac cagcaggatg
    19141 aacgccgaaa ccagcatccc gatgccaatc agaaaccatt tgccccagtt aatcgggcgc
    19201 gcgtcataac gcttcaattg ggtaacttcc gccattaatg acctaccaca cgccgaccaa
    19261 agcgactttg cagagtgtta attgagaaca gcagcagcag agatgccgcg aggatcaccg
    19321 aagcaatcgc gctcgctgcc gggtaatcaa actcctgtaa gcgcacaaaa atcatcagcg
    19381 acgtcacttc cgtcttccac gcgatatttc cggcgataaa aatcaccgcg ccaaattcac
    19441 caagactacg ggtaaacgac agcgccacgc ccgccaccag cgccggagaa agctccggca
    19501 gcaccacttt gcagaaactc tgccagcgcg ttgcaccaag cgtttccgcc gcttcttcat
    19561 attccgggcc taactcttcc agcaccggct gcacggtacg caccacaaac ggaatgctgg
    19621 taaaggccat cgccaccgca ataccaagcc aggtataggt gactttgata tcaaacttcg
    19681 ccagccattc accgtaaaaa ccgtttacgg aaaagagcga ggccagcgtt aaaccggcga
    19741 ctgccgttgg cagcgcaaag ggtaaatcca tcagcgcatc aagcagcgtg cggcccggaa
    19801 agcgataacg tgttaaaatc cacgccatca gcagaccgaa aacgccgtta aaaatcgatg
    19861 ccacaaacgc cgacagcagc gtcactttgt aggccgcgac cacctgcggg ttggtgatca
    19921 cctcccagta ctgcgcccag ctcatctcag aaagctgcat caccagcgca gagagcggca
    19981 gcagcaatat caggcacaca aacagcagac tggtgccaag gcttaaagta aagcccggca
    20041 gcacgcgtct tgaagaaact gcaaacatta cttacgcccc gccgctaaca gcttgtctaa
    20101 ctcgccgccg ctggtgaagt gggttttcat cacttccggc catgagccaa atttgtcttc
    20161 cacgcggaac agttcggtct gcgggaattt atctttcagt ttgtccatga cttccgggtt
    20221 attcacacgg tagtaatagt cggtgatgat ggtctgcgcc tgtgggctgt agagccagtt
    20281 caggtaggct tttgcggctt tttccgtacc gttggcctgc acatttttat cgacccacgc
    20341 caccgggaat tccgccagaa tattggtttt cgggatcacc acttcaaagc cctgtgcttc
    20401 atactgttta cggatgttgt tcacttccga ttcaaagcta atcagcacat cgcccaagcc
    20461 acgctcggca aaagtggtgg ttgcgccacg accgccggta tcgaacactt caacgttttt
    20521 caggaactgg gtcataaact gttcggtttt ggcttcatcg ccaccgtcag ctttatccgc
    20581 tgcgccccat gccgccagat aggtataacg cgcgttaccc gacgttttcg ggttcgggaa
    20641 aatcagcttc acgtcggagc gcaccaggtc gttccagtcg tggatattct tcgggttgcc
    20701 cttacgcacc aggaagccca tggtggagta gaacggcgag ctattattcg gcaggcgcga
    20761 ctgccagtca gccgggatca gcttgccttt gtcgtgcagg atttgtacgt cggtcacctg
    20821 gttataagtg acaacgtctg cttttaagcc ctgcaaaatc gccagcgcct gttttgatga
    20881 cccggcatga gattgtttta tcgtcagttt gtcgccgccg ttatcttttg cccattgctg
    20941 ttcaaacggc ggattcaggg cggcaaacag ctcgcgggag acgtcataag aactgttcag
    21001 cagttccgtt gcctgtacat ggcccgccag cagcagagaa gcgaccagcg cgagtgagtt
    21061 ctttttcagt aagttaacgg ccattgcgca cccttataaa tttaatgact ttctaatagc
    21121 catcatattt ataacggacg tgaaaggagt aacggtttta tataccgttt ggtgatttgg
    21181 aagttaaaaa gggaataaga acttggttca acaggcgtaa ttgttgcagg cagtttggac
    21241 acggacagcg cggagaaacc ggagcgtaca catagtacgt gaggatttcg agcactgccc
    21301 ggggccaaaa tgacaaatga aatagcctgg tgaacttagt tcaagacgaa atcctcccca
    21361 cacatgcagg gaggaaacag aggtgaatca gataccgacg ctaaccgtct ccggcagtgt
    21421 gctgccgcca tcaatcacat tctgtgtacc ggttaaatag ctggattcat ccgatgcgag
    21481 gaaggccgcc agttcgccga cttccagcgg atcggcgagg cgacacagcg ggattgcttt
    21541 tgccatttca gtcagcaccg attctggatc ttccgggttc gactggcggg caatgctttc
    21601 cgccattggc gtgcggacgt atcccgggca gatggcgtta acgcgaatac cggattgcgc
    21661 gtactccacc gccagcgatt tagtcaggcc aacaatcgcc gctttcgtta aggcatacgc
    21721 cgtttcgcca ggatcggcca ccatatcacc agtgactgaa gacatcatca caatgcgacc
    21781 atctttgcgc gcaatcatct ccggcagcac cgccttcgtg acgttccata cgcctttaat
    21841 attgatatca atatggaaat cgcgatcttc atcgctcata tcgaggaaac tgcccagacg
    21901 acaaacgcct gcgttattca ctaggatatc aatgcgccct tctttttcct tcgcgcgttt
    21961 gatagctgcg gctaccgacg ccgggtcacg cacatcggcg acaaccgccg tacagcgatg
    22021 accacgacca cacagttcgt ccgctagctt ttcgatctca ggggagatat ccagcaagat
    22081 taggttcgcg ccatgacgtg caaaagttct ggcaattcct tcgccaattc cctgcaatgc
    22141 gcccgtaatc agtgctgtct tgcccgtgag tttacccatt ttaatgctct ccttgttaat
    22201 acagagcgtt acactccatt ccctctgaaa atacaggata aacaaaatat tgctgcggat
    22261 cagctcacta attcaggaaa atctcaatcc agtcgctgct ttatgcatca tttcaggcgt
    22321 tgtgttagct cactgctgcg ctgaatcatt ttcaatgacg ccacatcatt gatgttgcac
    22381 aagaccaaca aacagtaaat cggtaacaga attttgcgcc gtgcgggtag acatcgacga
    22441 gctacgccat tcggtttcac cggaaacggt atcgagcgtg aaatgcgcca gccgccgcag
    22501 cggagagtca gccagagaag taatggcaat caccgttgcc ccctgctttc gtgccgcttc
    22561 tgcgcacaag acaatctctt ttttactgcc gctgtaagag atagcgattt gtacatcgcc
    22621 ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg tcggcttcgc aggccacgcg
    22681 atagccaatt ttcatcagtt tgaacgacag atcgcgcccc accagcgccg atccgcccag
    22741 gcccgttatc tggataaacg gtgctttact gatgacctca atgatttttt gcagccgcgc
    22801 gtagtcgaac aacgcacagg tttgctccag cgccagctct ttttcgcgat tcagtttgcg
    22861 ggcaatcact tccagcgaat catcactggt aatcgaactg tgcaggtgca gcgccgtggc
    22921 attcgttttc tcccggctgg cactgtattc gccaattaac gccatacgca attcagtaaa
    22981 tccttgcgcg ccgagcttct gggcaaattt cacaatgctc gactggctga taccgagctg
    23041 tttcgccatc tgccgtgaag agacggattt cagctcgctc actctggccc gcagaaagtc
    23101 agcaattttt tgctcgttcc cggtaaattc actttccgca ttgcgtattt tcgtcaggta
    23161 caacatcttc cactctcctg ggctggcagc aaaaattccg cttaagttct tatgacgctc
    23221 ttcacactct gcgagtgtag actcaatgat tcctttaagt ctgtcaattc gggatatatg
    23281 attccataca tctgcaatta tttgatcgta aatagtaagg tcaccaccga tgcaactaga
    23341 aaagatgatt actgaaggct cgaacaccgc ctcggctgaa attgaccgcg tatcgacgct
    23401 ggaaatgtgc cggattatca acgatgaaga taaaaccgta ccgcttgccg ttgatcgcgt
    23461 cctgccggat atcgccgcgg cgatcgatgt tatccacgcc caagtcagcg gcggcggtcg
    23521 tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg attctggatg ccagcgaatg
    23581 tccgcccacc tacggcgtga aaccggatct ggtggttggt ttgattgctg gaggcgaata
    23641 tgccattcag cacgcggtgg aaggcgctga agatagccgg gaaggtggtg ttaacgatct
    23701 gaaaaatatt aatttaacgg cacaggatgt ggtggttggc attgctgcca gcggtcgcac
    23761 gccgtatgtg attgccggac tggaatacgc acgccagctc ggctgccgca cagtgggaat
    23821 ttcctgtaat ccggggagcg ccgtttcaac caccgctgag tttgccatta caccgattgt
    23881 aggtgccgaa gttgttaccg gttcttcgcg gatgaaagca ggcacagcgc agaaactggt
    23941 gctcaatatg ctttccaccg ggctgatgat taaatccggc aaagtgttcg gcaacctgat
    24001 ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc caggtcaata tcgttaaaaa
    24061 cgccaccgga tgtaatgccg aacaagcgga agcggcgtta attgcctgcg agcgcaactg
    24121 taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc gcagaagcta aaaaacgcct
    24181 ggatcaacac ggcggcttta ttcgtcaggt tttagacaag gaataaccca tggccaaaga
    24241 gatcagcagt gaacttctga acaccattct tacccgtgtc ggcggaccgg gaaatatcgc
    24301 cagttgtggt aactgtatga cgcgcctgcg tctgggtgta catgacagtt cactggttga
    24361 tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt ttgaccagcg accaggtgca
    24421 ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa gcgatgagcg agctgctggg
    24481 cgaggcaccg gtacaggatg ccgcagaaat cgccgcccag aacaaacgtc agttaaaagc
    24541 caaacaaacc tccggcgtgc aacaatttct cgccaaattc gccaccatct tcacgccgct
    24601 gatccccggt tttattgccg ccggtctgct gctggggata gcgacgttaa ttgccacggt
    24661 gatgcacgtt ccggcagatg ctcagggcac gctacccgac gcgctgaatt ttatgaaggt
    24721 gttcagcaaa ggtttgttca ccttcctagt gattctggtg ggctataacg ccgcccaggc
    24781 atttggcggc acgggcgtca atggcgcaat tattgccgca ctctttttgc tcggttataa
    24841 ccccgctgca accaccggtt actacgccgg ttttcacgat ttctttggtc tgcccatcga
    24901 tccgcgcggc aatattatcg gcgtgctgat tgccgcgagg gcctgcgccc gcattgaagg
    24961 catggtgcgc cgctttatgc cggacgatct cgacatgctg ctgacctcgt taatcaccct
    25021 gctgatcacc gctacgcttg cgtacctgat cattatgccg ctgggtggct ggttattcga
    25081 aggtatgtca tggctgttta tgcacctgaa cagtaatccg ttcggttgtg cggttttagc
    25141 cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag ggctttattc ctgtttacct
    25201 cgcgttaatg gacagccagg gattcaacag cttatttccc atcctttcaa tggcaggcgc
    25261 gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg caaccgcaca gtgcgctacg
    25321 cagtcaggta cgcggggcga ttattcccgg cctgctgggc gttggtgaac cgctgattta
    25381 cggtgtcacc ctgccccgca tgaagccgtt tgttaccgcc tgtttaggcg gcgcggcggg
    25441 cggtttgttt atcggattga tagcctggtg gggtctgccg atgggcttaa acagcgcctt
    25501 tgggccgtct ggtctggtgg cgctgccgct gatgacttcc gcacaaggca tccttccggc
    25561 aatggcggtt tatgctggcg ggattctggt ggcatgggtt tgcgggttta ttttcaccac
    25621 gctctttggc tgccgtaacg tcaatctgga ctgatattat gaaacggaca atgctctatc
    25681 tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa ataccctgtt ctgacagaag
    25741 gtagttgatc aagtatatgc ggcgttaaag cagtaataaa aaggccagtc agtaatgtta
    25801 ctgcctggcc tttaatcaat gaatcagaaa cgcttacagc accatcaact tgtccagcga
    25861 cggtgcgaaa taatagccgc cggttaccgg tttggtgaaa cgcaacatcg catcacgttt
    25921 gccatcggta tcgccaaaca tgctcagcag ttgctgctca atgttatgca gacgcgcgca
    25981 gtaggcgcag aagtacagac cgtgagtgcc actggcggtg ccgtacggca ggctctggcg
    26041 aacaatcttc agccctttgc catcttcttt cagatcaacg cgggtgaggt gagaggtttc
    26101 cggacgttcg tcgccgtcaa tctcttcgtt ggcctctttg gtgcgcccga tcatcatctc
    26161 ctgatcgtga acgctcatcc ggttgagctg cttcaggttg tgctcccaac gctgcacaaa
    26221 cacatagctg ccgccggcat ccacgccgtc tttgataacc gccacttcgc ggcgcgtctc
    26281 ttcacccgcc gggttttctg taccgtcaac aaagccgctc aggtcacgct cttccaccca
    26341 acggaagccg tggatctctt ctttcacttc aatgcagtca ccaaaggctt ccatcgccgc
    26401 ctgagcgaca gagaagttta cgtcgtgacg cagagaaaga atgtggatca acacatcgaa
    26461 ctgggtcgtc ggcgcaaggc ctttaccgta gcccggaaaa tctttcagct cttctgcccc
    26521 aacgccgccg ctcagagcgc gccaggtgtt gttaccaaag gcaaccaccg caccaagatg
    26581 cgcgtccggg aatttcgctt caaaagttgc cagtttgtcg gcaaatgttt tactggccgc
    26641 acgcagggcg tcaacttccc ctttcacgtt ggcttcgatc caaatcgccg cgcggcaatg
    26701 ttctggcaaa atgccactct gaacctgaga cattgttcct cctgaaaata agaatgccac
    26761 gcaatccgtg gcattgttga gcgctatttt acgtgttttt tcgcgacggc ggtttgttca
    26821 gacgcaaatt aacgccgcca gataatttta ctgactttcc agtttttcag ggtatcgtcc
    26881 gaaggcatta acccttcagg accacgccat tccccgctga actggtagct aatatgttga
    26941 ctgccttcgg ctttacattc gacggcacga ttatcatcac catcagcttt ttggcaattg
    27001 ccaaatgctt tgctgtaaag gtcgctaaac ggtgtaccga ttttaacacc ggtgtcagca
    27061 ggaatatcgc tatccagcac gtcaatgcga ctgatcgtgc cttgatcgcc gttaatcacc
    27121 atcgccacgt tgtcgccttt catcacttca aaaaagcgca ccacgttgcc gttcgcggtt
    27181 ttcattccgc tgcgcaggcg ataatcgcca tcaagcgcat cagcaatggc ttgttcttgc
    27241 agtggtgtgg acgccgttaa ttcacccacg ccctgctcgc tcactttggt ggacgatcca
    27301 aaccagttcc acgggttagc ggcagaccag ttaactgacg acatcgtgga acagccggtc
    27361 agcatcaacg gcatagcgca taacattaaa cgcagcgatt tcatgtcact tcctttggtt
    27421 attcaataac gttgcttgga gtgcaaattc accaaaaagt gccgttattc tttttcttca
    27481 ttaaaacaag cgcgtaaacg tcgattggtc agcagccaga ttaacgccac aatatccgcc
    27541 actaccagcg ccagaccgat accgctaacg gattcaccgt tcagccacag ccacggttgc
    27601 cagcaaagta aaaccacctg cgccaacagc aacagaaaat agagcacatg ccaggtgcga
    27661 ggaaacgaag cccgccgacc gctcaacaaa aacgccagca ccgccggaat accgggaatc
    27721 agccccagcc agaaattatc gtgatcggga taaaacagat ttagcagcgc agtaccctgc
    27781 tcgcgcgacg caccggcaat gacaaacagc acccaggttc gcgcctgaag caatagcaca
    27841 agccagaaga gcaagggtaa acgcaggcgt ccgtgcgcat cataatggac aggatggaac
    27901 tcagtactct tcatcttcaa tcaaacgctt acccagactc agcacgtcgg cgtgttcata
    27961 tcccaggcgt tcatacattc cgagcaccat gtcgttatct tccggcacgt tgatctgaat
    28021 tttcgggcag ccgcgagcaa tcagcttttt ctccagccgg ttaagcaacg cattggcaat
    28081 tccacgccca cgaaactctg gatgcacgcc aagataataa gcagacccgc gatgcccgtc
    28141 ataaccgccc atcaccgttc cgaccacttc accgtttacc tcagcgacca aaaacaaact
    28201 gacgtcatgg ttcatcttac gctcgatgtc catttccgga tcgttccacg gacgcagcaa
    28261 gtcgcaacgc tcccaaaggg tgatgacctc ttcgaaatct tcctggcgaa atacgcgtat
    28321 ctccatggta ttcgttacct ttttgcgggt taaaaggctg attatggcgt gaacggtcga
    28381 attagccaat atctgacgaa aatcggttga aaaagtggca taatggggag ttgtcaacta
    28441 ttgaaatgaa aagtaaaaca attctcaaca gcaaaccgtc gtaacggatt acgcgatacg
    28501 atataacatc tggaacttta ttattacaac tcaggccgta tgagcacttt taaaccacta
    28561 aaaacactca cttcgcgccg ccaggtgctg aaagccggtt tagctgccct gacgttgtca
    28621 ggaatgtcgc aagccattgc caaagacgaa cctttaaaaa ccagcaacgg acacagcaag
    28681 ccgaaagcca aaaaatctgg cggcaaacgt gtcgttgttc tcgatccagg ccacggcgga
    28741 attgataccg gagcgatcgg acgcaacggt tcgaaagaaa aacatgtggt gctggcgatt
    28801 gctaaaaacg tccgttccat tttgcgtaat catgggattg atgcgcgttt aacgcgttct
    28861 ggcgatacgt ttatcccact ttacgatcgc gttgaaatcg cccataaaca tggcgcagat
    28921 ctgtttatgt caattcatgc cgatggcttt accaacccga aagctgccgg tgcttcggta
    28981 tttgccctct ctaaccgtgg ggcaagtagc gcaatggcga aatacctgtc tgaacgcgaa
    29041 aaccgcgccg atgaagttgc cggtaaaaag gcgactgaca aggatcacct attgcagcaa
    29101 gtactgtttg atctggtgca aaccgatact attaaaaaca gcctgacgct tggctcgcat
    29161 attctgaaaa agattaagcc ggtgcataaa ctgcacagcc gcaacaccga acaagccgcg
    29221 tttgtggtgt tgaaatcgcc gtcggttcct tcggtgctgg tggaaacctc gtttatcacc
    29281 aacccggaag aagagcggct gttaggcacg gcggcgtttc gtcagaaaat cgccacagcg
    29341 attgctgaag gcgtgatcag ttatttccac tggttcgaca accagaaagc acattcgaaa
    29401 aagcgataag ttatgaaacc cgacgcacac caggttaaac agtttctgct caaccttcag
    29461 gatacgattt gtcagcagct gaccgccgtc gatggcgcag aatttgtcga agatagttgg
    29521 cagcgcgaag ctggcggcgg cgggcgcagt cgggtgttgc gtaatggtgg tgttttcgaa
    29581 caggcgggcg tcaacttttc tcacgtccac ggtgaggcga tgcctgcttc cgccaccgct
    29641 catcgcccgg aacttgccgg gcgcagtttc gaggcgatgg gcgtttcact ggtagtgcat
    29701 ccgcataacc cgtatgttcc caccagccac gcgaatgtgc gtttttttat tgccgaaaaa
    29761 ccgggtgcag agcccgtttg gtggtttggc ggcggcttcg atttaacccc tttctatggt
    29821 tttgaagaag acgccattca ctggcaccgc accgcccgtg acctgtgcca gccatttggt
    29881 gaagacgttt atccccgtta caaaaagtgg tgcgacgatt acttctacct caaacatcgc
    29941 aacgaacagc gcggcattgg cgggctgttc tttgatgatc tgaacacgcc agatttcgac
    30001 cactgttttg cctttatgca ggcggtaggc aaaggctaca ccgacgctta tttaccaatt
    30061 gtagagcgac gtaaagcgat ggcctacggc gagcgcgagc gcaattttca gctctaccgt
    30121 cgcggtcgtt atgtcgagtt caatctggtc tgggatcgcg gcacgctgtt tggcctgcaa
    30181 actggcgggc gcaccgagtc gatcctgatg tcaatgccgc cactggtacg ctgggagtat
    30241 gattatcagc caaaagatgg cagcccagaa gcggcgttaa gtgagtttat taaggtcagg
    30301 gattgggtgt aactccctca cccccattcc cgcatccgct gatgcaacgt caacgacggc
    30361 ttctcggcaa acagctgctg gtaatccgtg gcaaattgcc ccagatgcca gaatccccac
    30421 tgcatggcgg cgtcttttac cgttgtgctt tgcgaccacg ggcttatcag ttcgcggcgt
    30481 acggcgttca ggcgaatgcg tttcagccac gcgtttgggc caatgcctaa aatagcgtga
    30541 aacgcgtttt gtagcgtgcg gcgactgaca tgcagttgat tacacaaatc cagcaccgtc
    30601 accggttcgg acatgttttc cagcacatat tcacgggcgc gggaaagcaa ccgacggtaa
    30661 ctctgatgac tgatgctttc cgccgtcacc attggttgcg cttcttccag catcgccccc
    30721 atcgccatta gcaaattatc ccccagcact tttcgcactg caggctgatg gagattttcc
    30781 gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc cccacagcgc ggctttatgc
    30841 tgctctttca cttccagcgc cgactggcta cgcagcatat gtaatacccg atccggattg
    30901 tgcaaaaagt tagcctgaca ggtgatgaca tcttcagaca gcactacgcc caggatcgta
    30961 taatcgtccg gcgtacttaa ttcaaactcg gttccaccgg ggcgcgttgc tatttctgcg
    31021 cttcccagac attgcgaacc gataaatccc tgctcaccgc gcgtcgccgg aatgccaaac
    31081 cagaacgagt tcggccagac caggcacgac tgacgcagcg ccagaccggt gtattcacga
    31141 aaaacctgaa tatcatcgag tagaatttcc gtaaattcac catgaaactt gcccggatgc
    31201 agctgatcgt aaatctgctg ccaggcggta atcgtcaaag catgttcata gacatccgtt
    31261 gtctgtcgtt gatgaacatt atccacttcg accttcggcg tgagcttcag gttttcgggt
    31321 aagggttcat gataaagatg gtgcaaattg gctgtacggg tctttttcat gatgttaatg
    31381 ccgggtgttg taggacaccc ggcacctccg acaggttaat ggggcttgag acgataacga
    31441 ctactgcgtt tacgttacgt cccggcagaa aagagctgtt cgagcgcatt tctggctttt
    31501 tccagcggcc agccaaagtg ggcggccact tctcctgccg tcattccctg acatactgat
    31561 gttaacagcg ccaacaactc gtcagcagat tccgcgacga taactggcgc gtcgggttcc
    31621 tttacgggtt gcttcggctg gcggttaaag ccagtaacca gccactgggt gtcatcgtct
    31681 ggccgaccga tctccttacg gctgatgaca cgcccggtac gcatcgcggc agcacaacct
    31741 gcgtccagcg ccgcacgaca cgccgccaga tcgccttcca ccaccagcgt taagcgacca
    31801 gggtcaagca cttcgtgact gagcagacga acgttagctg ctttgagcat ggcatccgct
    31861 gcatctatcg cggcgaccat gccgtccact tccagcaatc ccagtgcatt gatcatcggc
    31921 aacctccgtt acgcacgctg gattggatta cgcgcgattt ccagcactgc atcggtaaag
    31981 gcgttacagg ctgctttaca cgcggcctgg ctaccggtta aaaatgctgc cgagtagttg
    32041 gtttcagacg gtggcgggac gtaggtcacc agctgtacgt cggcagattt caacgcggca
    32101 tcaatgccgt aggtcgcttc cagcggcggt gccaccagat acgccatcgg atcgccaagc
    32161 gtgatcccgg cggttgatga aagataagaa cctgtacgcg aaaccacatg cgccaggaag
    32221 gcggtgtttt ctgcgtcatt ggcccactgg aaagctgcgc cattttcaat atgtgcaacc
    32281 atcgcatcca gaccggcacg cacttccgcc gggtttggcc caccaagcat aatcagcact
    32341 tcaccggcag tcggtgacgg gccgtgtgcc gctcccgcat acagcgagcg accatatacc
    32401 acttcgacca tcgcttgttt ggtcgcttcg tcagccgcaa tgtaggttac gtcgtcagaa
    32461 tcggcagaaa tcagcccgag gctacgaata tgcggcggca attttagttc acgcgcaaat
    32521 tcagcgttaa cggaggcaat cacccgcatg gcggttaccg acggtcgaat caaatctaaa
    32581 gctggcatga tgcctcctta tcgggtcatg ttgatgccgg atgctttctg ctccagcata
    32641 cgtttcgcca aatccacaat gacggctgcg gcttcaaccg gcggcgtgcc gccctggtga
    32701 atgttagaga tacaggtgcg atcggcctca acggtggtcg ccatacgcgg cgagtaaacg
    32761 gcgtagcagg agaggctttc tgactgccct aaccccggac gttcccccac cagcaggatc
    32821 accactttcg cgccgaggat ctcaccgatc tgatcttcaa tcttcacgcg accataacga
    32881 acaaagaatg gcgtgccgac tttcagcccg gcctgtttca ggcccgccat cagcggcggc
    32941 aggatctctt cgtagttgac ggtgatggca tcagttgaca ggccatcaga aataacgacc
    33001 tgtacgcccg gattagcaac acactgtgct ttcagcgctt caacagcttc tgcacacagg
    33061 cggcggccca tatccgggcg agtcaggtag agatttttgt cgctaatttc agagcgtact
    33121 tccagcaagc cctgcgcttt cacccactct tccggcactt ctttcagtac ggtatctttc
    33181 gaacgagagt gatcggccaa gaaacgcagc agcgcctgag tacgtggacg cggaccggca
    33241 cgaccggtac aaacgcgggc cacggtgctg cggcgcagtt ctgttaatac gtctgcgcga
    33301 tgcgggtttt caacaccaat ccacgctttt gcttcagcgg aacctaaatc cagcgcacag
    33361 ctttccgagg tcaccggtgc cgcacagttg gtggtggcgc actttgcttc tgacggcgcg
    33421 ggggccgctt gtcccattga cgccatcacg ctgcgtacaa tttcttcaat ctgtttttga
    33481 tccatgatgt gttatctccg cgtcatcaga agaacagtga cggatcgccc gcccgtttgg
    33541 tcaggcgacc gtttgccata atgcccatgc tttccagcca gcgttcaaac tctggtgacg
    33601 ggcgcaggtt gagtaactga cgcacagtgg cggtatcgtg gaatgcggtg gtctggtagt
    33661 tgagcatgat gtcatcaccc agcggcatcc ccatgatgta gttgcagcct gcggtggcga
    33721 gcaggatcat caggttttcg ttgaggttct ggtcagagtc agcgtggttg gtgtagcagc
    33781 cgtcacagcc catagagatg ccgctcagct tgcccataaa gtgatcttct aagccagcac
    33841 ggataatctg gcggtcgttg tagagatact ccggcccaat aaagccgacc acggtgttga
    33901 cgataaacgg atcgtaatga cgcgccagcc catagttacg tgcttccatc gtcacctggt
    33961 cagcgccgaa gttagcgcca gcggatagcg cagagccttg tccggtttcg aagtagaggc
    34021 agttttcccc ggcgatacga ttgaactctg cgcccactgc gcgcgcttcg tcgagcatcg
    34081 ccagttccac gccaaactct ttcagccctt tttcgctgcc acagatactc tggaaaatca
    34141 gtccgccagg agcgccgcga cgaatcgctt cgatctgggt ggtgacgtgc gccagtacgc
    34201 agccctgagt tgggatgttg aatttgtcga tcacgccata aatggtatcc agcacgcggc
    34261 ttaagttttc cacgtcgtca gttaccgggt taacgccgat caccgcatcg cccaccccga
    34321 aggaaagccc ttcatagatt tgcgcagcga tactttgcac gtcgtcacgg gtatcgttcg
    34381 gctgcaaacg ggcgctaaag gtgcccggaa taccgatggt ggtattggcc tttttgatta
    34441 ccggcatttt cttcgcgccg tagatcaggt ccgcgttgga gcaaatcttc gctatcgccg
    34501 cgaccacttc cgaggtcagc cctttgcggg taaaggcaat gtcgtccacg ctggtttcat
    34561 cgctcagcac atactcacgc agttcgctga tgctccagtt tttaatctgg ttgtaggccg
    34621 tttcgttaac gtcgtcctga atcagccgcg tcacgcagtc atcttcatag gcaatcaccg
    34681 gattattgcg gatgtccgct acggtcattt ccgacaacac ctgctttgcc gccacgcgct
    34741 cctgtgagct tgccgctgca acgcccgcca gcacatcccc cgaacgcagt tcgttggctt
    34801 tagccagcac ctcttttaca tccttaaact gatatacatt gccgaacaat gtggtcttta
    34861 gtttcataag tcgttccctc aggaagaagt gatgagtaac atttctcaca gcctgcaaac
    34921 aattctattt catcgttagc ccttgtcatg taaggtgatt tccttaacac gcccgttact
    34981 tatcctgccg aacgcaaccg aactcgccgg atacatgagc ggggttatcg agtcgttaga
    35041 tcgaggtatc cggttttgag caaaacacca ctcacagcaa aggccataga cgccgcacaa
    35101 ccaaaggaca agcaatacaa actcaccgct tcactgacgc ccggcctgtt tttgctggtg
    35161 catcccaacg gcagcaaata ctggcgattc cggtactgga tagacaagaa agagcggtta
    35221 caggctattg gggtatatcc gctgattagc ctcaaggaag cccgcaaacg cgccacagag
    35281 agccgtttac tgatagccca gggaattgac ccaatggatt tggcacgcaa ggagaaagcc
    35341 attgatgcac tcaacatggc ggcaggtttt aagacggtag cggaggactg gctggctacc
    35401 agggtaggcg gctggagtga gagctataca aagcaggtca aatcagcact ggagaaagac
    35461 gtttatccgg tacttggcaa gcgttcaatc gttgatataa ccgcccgtga cgtgctggca
    35521 ttactccaga agaaagaacg taccgcaccg gagcaggccc gcaagctacg ccagcgcatc
    35581 ggtgagatct tcaagtttgc cgttatcacc gaactggtaa cgaggaatcc ggttgcagat
    35641 cttgatacgg cactgaaggc ccgccgacct ggtcacaacg cgtggatacc gattagtgaa
    35701 attccggcct tctacaaagc ccttgagagg gccgggagcg tccagattca ggcggcaata
    35761 cgtttgctta tattaacggc attaaggacc gcagaactgc gtttgtgccg ttgggaatgg
    35821 ataaacctgg aggacgcaac aatcacattg cccgctgaag tcatgaaggc ccgccgcccg
    35881 catgtggtcc cgctatcacg gcaggcggtc gagctattgc aggaccagtt cacacgcagc
    35941 ggatacagtg ttttcgtgtt cccgggccga ttcatggata agccattatc agccagcgcg
    36001 atactcaagg cgcttgagcg tatcggatac aagtcaatag ccacaggcca cggctggcgc
    36061 acaacgttca gcaccgcact taacgaaagc ggcaggtata accctgattg gattgagatc
    36121 caactagccc acgtcccgaa gggaattagg ggagtttaca accaagcggc ctatctcaaa
    36181 atgcgccgtc aaatgcttca ggactacgcc gacgcgattg attcgatact ggctgatggt
    36241 atcaatccgc ttgaactgta atgaatgatt aatcacgttt tccgacgact ccaccggagt
    36301 aggccggata aacactggac accttcaaaa taactattca cttttgccgg attcgtccgg
    36361 ctttttttgt ctttgccgat gggatcaatt attccaatta ttccaattat tccaattatt
    36421 ccaattattc caattattcc agtattgttg gaataaatga ataaatatca atgacttaaa
    36481 gccgtgtttg caacagggtg gatcggggat cgaaaatagg atcaacacaa aaacaccctg
    36541 gaggctacag cacactaggg ggacacaaaa acaacacagg aaaaaaatac catgtaccaa
    36601 tcagcaaccg cctacgtggc gaaaccaggc gaaacactgg tcaaacacaa aattgcaaca
    36661 ctccaagcac ttcaaacact ccaagccctt ccggaacagg ttcaggccgc ctttcgtgat
    36721 ggcatgaaca tcatccgctt cgatgggtat cgctgggaat acaagccagc aactggattc
    36781 gtgcgcatag atcaaatctt gtcgccagcc aatgaagagc cagcgctagc caatgcagaa
    36841 ccagcgcagg agcaggagca ggagcaggag caggagcagg agcaggagca ggagcaggag
    36901 caggagcagg agcaggagca ggagcaggaa cagaataaag cggcggctat tgccgagttc
    36961 aaggagctgc aaagacaaca aatcacagcg cagcaagaag aatgccgcca cccgctggca
    37021 atgcccaagc cagtagcgaa agccaacaca gacaagccga cttcatggtt taacgaagaa
    37081 gcggaagaag atttgcgttt tgatagtaaa gaggagcagt tgcctgaaga gcgggaacag
    37141 gtggaaacgg aagaccagca aaacgattat cgcaaccttc cagccgtcaa taacgctatg
    37201 gcttacctct tcaaaagcgg gctgtttcac aagctgaaag gcgatgagtt aaagctatat
    37261 acgtggatgg tatccaagtg caggtatcgc ggtaacgaca aatatgagat ttggtattcc
    37321 cgcaccaaaa tggcaaaagc gttaggtaag agcgttgaca cgatagatcg ccacctggaa
    37381 gcgttgagaa agctgggact tatccaggaa atgcgccagg cactgagggg caaatgtgga
    37441 aaaacctaca gactgcgaat cccgtcaaaa ggcacagcag aagaggcctc aatgacggca
    37501 cagcagcacg aaagagaagc aaaagtcagc tacctggtac actaggcagc gggtcagcaa
    37561 aggggggcaa atgccccttt tttcatgtca gataatcaga tcattacctc aataaactaa
    37621 taatcacatc gcggttacaa ccagtttgat agtgatagct caaacccaga cgtggcgctg
    37681 gctgcaagca aaataagccg caaaaatgcg gtcctatttg ccagaacctt ttattattca
    37741 attggttagt gcaagaaaaa gcgtgattac tgttcaagaa gtatccagaa aggtcatgat
    37801 tttgccatga ttttgacgct gaaaaaaaat aggtgggcat ttttgcccac tcactagccg
    37861 caaatttgcc cactcactag ccgcattttt gcggcccaaa gtaataaata cagagaaata
    37921 ataaatacag atcagcgcca agatttttcg tcttttcggc ggaatcatga gactagtgct
    37981 tacgtcctga taacctccag agcagacctt aacttctgtg cagcaggact acgctcattg
    38041 ccgctcggct aggtcaaatc ctcacgataa agctgttcgg attgtgacca taatgttaaa
    38101 ggactcttta aatcattcct tgacgaatta actagctggc ttaagcggaa tagcatgcag
    38161 gataaaaaca aaaccagctt agcagtgggg agagcttcgc ttcgctcgtc tcctggggcc
    38221 aatgctcaat gaaacgacgt aaggcacgga gcgctcacca taggtagaca cataaacatc
    38281 tgagagcgca ccttctatcg cgctacgcgc tcaataaaaa ccaacgacac cgccacactg
    38341 caagaccaat ggtttttatc gcgtatacag accagacagg aaacagcagc aaagcagcgt
    38401 atagccaggt gcagacagca cactcacacg acgcacagca ggcttaagtg atcactcagg
    38461 aaactttatc cccgccgccg atcaccgcga actatcatga ccgcgccaaa ttcgaaagga
    38521 atggcccata gaacgcgatg aacacacata agcgctaact tgcttacctg aaaacttaac
    38581 gtcgccacag agcgattgag agcgcttaaa atcgatgcga tttcattaaa tttttctcac
    38641 tcgcctgaat ggtggcgatc aggactgatc gcaacattgc cccgcctcga tattgagcgc
    38701 ataatagata catacattaa gcggaaaaga gataagttta ttgtgaaaat cagggggtta
    38761 cacatcatgc gcatatcaga acaacaacgg cagatcctcg actatctggc aaaaaacgaa
    38821 tcaatcaccg cacgacaagc cgcagatctc gtttatggtg gcaatgttac ccgcgtccag
    38881 gttgacgccg cccgccgctc actcatgacg atggttagta atgggctaat caggaaacaa
    38941 ggccgcgctt tcgtagctga aagacatatc aatcacgctt atagccagga cgttgaagaa
    39001 ttgcgccgcc agatcctttg ggtattcagc aaacagggtc aggagtattt cctttctgaa
    39061 cacccaaatt tcgccgccaa agagttaaca gtaagcggat tgtcgtgcgc actggattat
    39121 cttgtcggtg aaggttgcgt tacttacaag cagctatggc acgccctgaa tacgcttgta
    39181 gaagatggtt tactcattgc cacgcgtgag aacatcgaat actggataat gccgcgttca
    39241 ggtcgccgca atcatgcacg gtatatttca ggcaacagat acagaacgcc ggactttaat
    39301 ccgacacagg aagatcggga ccgtgccgaa atgatgtgca gggaatagga gaaaagaatt
    39361 atggataagt tattttcgat gcagtgaaca caagcgggcg caatgcccgt ttttattgcc
    39421 ttattccacg cctgacgaat caatttgatc acacctgagc aaatgcatag ccagaagtta
    39481 aggccgttat attgcgattg ggagcgtttt aaacggtatc aatttaatta attctgatgt
    39541 ggtgatttgc aggcaaaaaa aacccgccgg agaagcggga aatagagaca tatcgttgta
    39601 gttattaaca gatcactttg aggtcgaggg cggctaaccc tcgctaatat tatcccgcca
    39661 ttaccttgcg ggtgcaaata atcttttatt gctttgatga tttaataaat agcacttttt
    39721 gctaacaaaa accgctgtca atatatggcc aaaaatgtca acagaaaaaa taaggtttat
    39781 aatattaagt atcacatggg taatgggttc agggaatcgg aatccaccgc ctcacggaaa
    39841 ttcaaaattt gcttcttctt tcctccgtgg ggcggcttct ttttcgtatc gaggtattta
    39901 tgaaagcaat aacattagaa cagttcgcag caatggcgaa tgtatcgacc agaacgctga
    39961 ggcgcaggct aaaaaaacca tcaaccggag tccctttgcc atttacccca cgcggtactc
    40021 gccaattatt ctgggagcac gaatgcaaag aatggttgta cagaacagaa cgataataat
    40081 cacgcatcac attttgaggt aaacatcatg accaatatcg aaagattaga acgctgtgcc
    40141 gatattatgc ggcgcagatg gatttatgac cctgaagcgg gtttattgtt tagccgtgaa
    40201 acaaaagacg ttatcaaagg cagcatgacc aataaagggc atttggttgt aacggtacat
    40261 gaaggtaaat tcaaagtgac actcacctat cagaaagcct gctatgtata cacatacggc
    40321 gcttatgacg agacattata tgaagtg
//
LOCUS       DADMFL010000023        39587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.023, whole genome shotgun sequence.
ACCESSION   DADMFL010000023 DADMFL010000000
VERSION     DADMFL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.023"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(11..322)
                     /locus_tag="JNZ27_001228"
     CDS             complement(11..322)
                     /locus_tag="JNZ27_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY8663632.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGCTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JNZ27_001229"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNZ27_001229"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JNZ27_001230"
     CDS             701..1054
                     /locus_tag="JNZ27_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY8663633.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNZ27_001231"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNZ27_001231"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY8663634.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JNZ27_001232"
     CDS             complement(2810..3280)
                     /locus_tag="JNZ27_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663635.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNZ27_001233"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNZ27_001233"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY8663636.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNZ27_001234"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNZ27_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY8663637.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNZ27_001235"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNZ27_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY8663638.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JNZ27_001236"
     CDS             complement(5283..8432)
                     /locus_tag="JNZ27_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY8663639.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNZ27_001237"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNZ27_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY8663640.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JNZ27_001238"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JNZ27_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY8663641.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JNZ27_001239"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JNZ27_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY8663642.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNNEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNZ27_001240"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNZ27_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY8663643.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNZ27_001241"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNZ27_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY8663644.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JNZ27_001242"
     CDS             14911..15288
                     /locus_tag="JNZ27_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY8663645.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JNZ27_001243"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JNZ27_001243"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY8663646.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATAKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JNZ27_001244"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JNZ27_001244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY8663647.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JNZ27_001245"
     CDS             18099..18428
                     /locus_tag="JNZ27_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY8663648.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JNZ27_001246"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JNZ27_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY8663649.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JNZ27_001247"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JNZ27_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY8663650.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JNZ27_001248"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JNZ27_001248"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY8663651.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JNZ27_001249"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JNZ27_001249"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY8663652.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNZ27_001250"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNZ27_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /locus_tag="JNZ27_001251"
     CDS             24045..25349
                     /locus_tag="JNZ27_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY8663653.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="JNZ27_001252"
     CDS             complement(25482..27158)
                     /locus_tag="JNZ27_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY8663654.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /locus_tag="JNZ27_001253"
     CDS             complement(27396..28616)
                     /locus_tag="JNZ27_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663655.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /locus_tag="JNZ27_001254"
     CDS             28834..30486
                     /locus_tag="JNZ27_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8663656.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNZ27_001255"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNZ27_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY8663657.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="JNZ27_001256"
     CDS             complement(31206..32000)
                     /locus_tag="JNZ27_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663658.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="JNZ27_001257"
     CDS             32138..32479
                     /locus_tag="JNZ27_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8663659.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNZ27_001258"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNZ27_001258"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY8663660.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="JNZ27_001259"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="JNZ27_001259"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY8663661.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="JNZ27_001260"
     CDS             36395..37687
                     /locus_tag="JNZ27_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8663662.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGVGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMGNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /locus_tag="JNZ27_001261"
     CDS             37812..38219
                     /locus_tag="JNZ27_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY8663663.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="JNZ27_001262"
     CDS             complement(38220..38678)
                     /locus_tag="JNZ27_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY8663664.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..>39587)
                     /locus_tag="JNZ27_001263"
     CDS             complement(38675..>39587)
                     /locus_tag="JNZ27_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY8663665.1"
                     /translation="IFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPGLS
                     LVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVSNL
                     ELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIRDV
                     RPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSAFL
                     QAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVMMP
                     LEASSLMGSIAGIAELVKDSANKRTQP"
BASE COUNT         9704 a        10305 c        10300 g         9278 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgcagcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttta tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgtgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgctgat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg atctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacaacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgctaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccacag cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc agacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggt
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatgggca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagata
//
LOCUS       DADMFL010000024        39560 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.024, whole genome shotgun sequence.
ACCESSION   DADMFL010000024 DADMFL010000000
VERSION     DADMFL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.024"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..186)
                     /locus_tag="JNZ27_001264"
     CDS             complement(<1..186)
                     /locus_tag="JNZ27_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY8663666.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="JNZ27_001265"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="JNZ27_001265"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY8663667.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="JNZ27_001266"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="JNZ27_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY8663668.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="JNZ27_001267"
     CDS             3863..4144
                     /locus_tag="JNZ27_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY8663669.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4481..4978
                     /locus_tag="JNZ27_001268"
     CDS             4481..4978
                     /locus_tag="JNZ27_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8663670.1"
                     /translation="MSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPT
                     TVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTV
                     GYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVM
                     RVAQD"
     gene            5059..5733
                     /locus_tag="JNZ27_001269"
     CDS             5059..5733
                     /locus_tag="JNZ27_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8663671.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5749..8229
                     /locus_tag="JNZ27_001270"
     CDS             5749..8229
                     /locus_tag="JNZ27_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8663672.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLGVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8245..9278
                     /locus_tag="JNZ27_001271"
                     /pseudo
     CDS             8245..9278
                     /locus_tag="JNZ27_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9360..9698)
                     /gene="rcnB"
                     /locus_tag="JNZ27_001272"
     CDS             complement(9360..9698)
                     /gene="rcnB"
                     /locus_tag="JNZ27_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY8663673.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9917..10765)
                     /locus_tag="JNZ27_001273"
     CDS             complement(9917..10765)
                     /locus_tag="JNZ27_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY8663674.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVINWQILLFGLTGGLIPCPATITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            10886..11158
                     /gene="rcnR"
                     /locus_tag="JNZ27_001274"
     CDS             10886..11158
                     /gene="rcnR"
                     /locus_tag="JNZ27_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY8663675.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11250..11346
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11381..12169
                     /gene="thiM"
                     /locus_tag="JNZ27_001275"
     CDS             11381..12169
                     /gene="thiM"
                     /locus_tag="JNZ27_001275"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY8663676.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12166..12966
                     /gene="thiD"
                     /locus_tag="JNZ27_001276"
     CDS             12166..12966
                     /gene="thiD"
                     /locus_tag="JNZ27_001276"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY8663677.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13031..13849
                     /locus_tag="JNZ27_001277"
     CDS             13031..13849
                     /locus_tag="JNZ27_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY8663678.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13901..14647
                     /locus_tag="JNZ27_001278"
     CDS             13901..14647
                     /locus_tag="JNZ27_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8663679.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDSSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14621..15586)
                     /locus_tag="JNZ27_001279"
     CDS             complement(14621..15586)
                     /locus_tag="JNZ27_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8663680.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15583..16586)
                     /locus_tag="JNZ27_001280"
                     /pseudo
     CDS             complement(15583..16586)
                     /locus_tag="JNZ27_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16583..17059)
                     /locus_tag="JNZ27_001281"
     CDS             complement(16583..17059)
                     /locus_tag="JNZ27_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663681.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            17213..18265
                     /gene="fbaB"
                     /locus_tag="JNZ27_001282"
     CDS             17213..18265
                     /gene="fbaB"
                     /locus_tag="JNZ27_001282"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY8663682.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18573..19427
                     /gene="gatY"
                     /locus_tag="JNZ27_001283"
     CDS             18573..19427
                     /gene="gatY"
                     /locus_tag="JNZ27_001283"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY8663683.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19456..20718
                     /gene="gatZ"
                     /locus_tag="JNZ27_001284"
     CDS             19456..20718
                     /gene="gatZ"
                     /locus_tag="JNZ27_001284"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY8663684.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NNSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            20728..21180
                     /gene="gatA"
                     /locus_tag="JNZ27_001285"
     CDS             20728..21180
                     /gene="gatA"
                     /locus_tag="JNZ27_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY8663685.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21211..21485
                     /gene="gatB"
                     /locus_tag="JNZ27_001286"
                     /pseudo
     CDS             21211..21485
                     /gene="gatB"
                     /locus_tag="JNZ27_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
     gene            21489..22845
                     /locus_tag="JNZ27_001287"
                     /pseudo
     CDS             21489..22845
                     /locus_tag="JNZ27_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            22893..23933
                     /gene="gatD"
                     /locus_tag="JNZ27_001288"
     CDS             22893..23933
                     /gene="gatD"
                     /locus_tag="JNZ27_001288"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8663686.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24033..24812
                     /locus_tag="JNZ27_001289"
     CDS             24033..24812
                     /locus_tag="JNZ27_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8663687.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(24894..25793)
                     /gene="yegS"
                     /locus_tag="JNZ27_001290"
     CDS             complement(24894..25793)
                     /gene="yegS"
                     /locus_tag="JNZ27_001290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY8663688.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            26199..26516
                     /locus_tag="JNZ27_001291"
     CDS             26199..26516
                     /locus_tag="JNZ27_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663689.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(26858..28219)
                     /locus_tag="JNZ27_001292"
     CDS             complement(26858..28219)
                     /locus_tag="JNZ27_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY8663690.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28366..28698)
                     /locus_tag="JNZ27_001293"
     CDS             complement(28366..28698)
                     /locus_tag="JNZ27_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY8663691.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(28889..29611)
                     /gene="baeR"
                     /locus_tag="JNZ27_001294"
     CDS             complement(28889..29611)
                     /gene="baeR"
                     /locus_tag="JNZ27_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY8663692.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(29608..31011)
                     /gene="baeS"
                     /locus_tag="JNZ27_001295"
     CDS             complement(29608..31011)
                     /gene="baeS"
                     /locus_tag="JNZ27_001295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY8663693.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(31008..32423)
                     /locus_tag="JNZ27_001296"
     CDS             complement(31008..32423)
                     /locus_tag="JNZ27_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663694.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(32424..35489)
                     /gene="mdtC"
                     /locus_tag="JNZ27_001297"
     CDS             complement(32424..35489)
                     /gene="mdtC"
                     /locus_tag="JNZ27_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY8663695.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIVQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(35490..38612)
                     /gene="mdtB"
                     /locus_tag="JNZ27_001298"
     CDS             complement(35490..38612)
                     /gene="mdtB"
                     /locus_tag="JNZ27_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY8663696.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(38612..>39560)
                     /locus_tag="JNZ27_001299"
     CDS             complement(38612..>39560)
                     /locus_tag="JNZ27_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY8663697.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         9916 a         9689 c        10247 g         9708 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggcgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4201 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta atcaatagat
     4261 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4321 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4381 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4441 aacgttcaat tattgctgcc gctgtctttt cttctttttt atgagcgctg gagtatttgc
     4501 tgcagacgtt gataccggaa cattaaccat taaaggaaat attgcagaat ctccgtgtaa
     4561 attcgaagcg ggtggtgatt cagtaagtat taatatgccg actgtaccaa ccactgtctt
     4621 tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc ggtgtaacca gcagcatgtt
     4681 aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc tcgttgatca caaacgacaa
     4741 aataaccggt aacgataagg cgatagccag tagcaacgat accgtgggtt actatctcta
     4801 tttaggtgat aacagcgatg tcctagatgt ttctgcacct tttaacattg agagttataa
     4861 aacagcagaa ggtcaatatg ctattccgtt taaagcaaaa tacctgaaac tgacagataa
     4921 ctcagtgcaa tcaggtgatg tgttatcttc tctggttatg cgtgtagcgc aggattaata
     4981 aataaaattg caagtttctc caggtaataa acatggccgc tatccccatg gcggcttttt
     5041 aatttaagag gcattaagat gaaaggatta ttatctttac tcattttttc tatggtcctt
     5101 cctgcacatg ccggaattgt tatctacggg acgcgcatta tttacccggc agaaaataaa
     5161 gaagttatgg tgcagttgat gaaccaggga aatcgttctt cgctgctgca ggcgtggatt
     5221 gatgatggcg atacgtcgtt accaccagaa aaaattcagg ttcctttcat gttaacgcca
     5281 ccagtggcaa aaataggggc aaattccggg cagcaagtaa aaatcaaaat tatgccgaat
     5341 aaactgccca ctaataaaga aagcattttt tatctgaatg ttctggatat tccaccaaat
     5401 agtccagagc aagaaggcaa gaatgcactg aagtttgcga tgcaaaacag aattaagttg
     5461 ttttaccggc cagcgggtat tgctccggta aataaagcga cctttaaaaa gttactggta
     5521 aatcgcagtg gcaatggatt ggtgataaaa aatgactcag ctaattgggt gacgatttcg
     5581 gatgtcaaag ctaataatgt taaagtcaat tatgaaacta ttatgattgc ccccttagaa
     5641 agtcagagtg ttaatatcaa aagtaataat gcaaataact ggtatctgac cattatcaat
     5701 gaccacggca actatattag tgacaaaatt taattgcagg agctgcctat gttgagaatg
     5761 accccacttg catcagcaat cgtagcgtta ttgctcggca ttgaagctta tgcagctgaa
     5821 gaaacctttg atacccattt tatgataggt ggaatgaaag accagcaggt tgcaaatatt
     5881 cgtcttgatg ataatcaacc cttaccgggg cagtatgaca tcgatattta tgtcaataag
     5941 caatggcgcg ggaaatatga gattattgtt aaagacaacc cgcaagaaac atgtttatca
     6001 agagaagtta tcaagcggtt aggcattaat agcgataact tcgccagcgg taagcaatgt
     6061 ttaacatttg agcaacttgt tcagggtggg agctatagct gggatatcgg ggtttttcgt
     6121 ctcgatttca gtgtcccgca ggcttgggtg gaagaactgg aaagtggcta tgttccaccg
     6181 gaaaactggg agcggggtat taatgcgttt tatacttctt attatgtgag tcagtattac
     6241 agcgactata aagcgtcggg taacaacaag agtacatatg tacgttttaa cagcgggtta
     6301 aatttactgg agtggcaact gcattctgat gccagtttca gtaaaacaaa taacaatcca
     6361 ggggtgtgga aaagcaatac cctgtatctg gaacgtggat ttgcccaatt tctcggcacg
     6421 cttcgcgtgg gtgatatgta cacatcaagc gatatttttg attctgttcg cttcagcggt
     6481 gtgcggttgt ttcgtgatat gcagatgttg cctaactcga aacaaaattt tacgccacgg
     6541 gtgcagggga ttgctcagag taacgcgctg gtaactattg aacagaatgg ttttgtggtt
     6601 tatcagaaag aggttcctcc tggcccgttt gcgattacag atttgcagtt ggccggtggt
     6661 ggagcagatc ttgatgttag cgtgaaagag gcggacggct cggtaaccac ctatctggtg
     6721 ccttatgcag cggtgccaaa tatgctgcaa cccggcgtgt cgaaatatga ttttgcggcg
     6781 ggccgtagcc atattgaagg ggcgagcaag caaagtgatt ttgtccaggc aggttatcag
     6841 tatggtttta ataacttatt gacgctgtat ggcggcacga tggttgctaa taattactat
     6901 gcgtttaccc tcggaacggg ttggaacacg cgcattggcg caatttccgt cgatgccacg
     6961 aagtcgcata gtaaacaaga caacggcgat gtgtttgacg ggcaaagtta tcaaattgcc
     7021 tacaacaaat ttgtgagcca aacgtcgacg cgttttggtc tggcggcctg gcgttattcg
     7081 tcgcgtgatt accggacatt taacgatcac gtttgggcaa acaataaaga taattatcgc
     7141 cgtgatgaaa acgatatcta tgacattgcc gattattacc agaacgattt tggccgtaaa
     7201 aatagctttt ctgccaatat gagccagtca ttgccagaag gctggggttc tgtgtcctta
     7261 agtacgttat ggcgagatta ctgggggcgt agcggcagca gtaaggatta tcagttgagt
     7321 tattccaata actggcggcg gataagctat accctcgcgg caagccaggc gtatggcgag
     7381 aatcatcatg aagagaaacg ttttaatatt tttatatcaa ttccctgtga ttggggtgat
     7441 gacgttacga cgcctcgtcg gcaaatatat atgtctaact caacgacgtt tgatgatcag
     7501 ggttttgcct caaataatac gggattatca ggaacagtag ggagtcggga tcagttcaat
     7561 tatggtgtca acctgagtca tcaacatcag ggaaatgaaa cgacagctgg ggcgaatttg
     7621 acctggaacg cgccggttgc gacagtgaat ggcagttata gtcagtcgag tacttatcga
     7681 cagactggag ccagtgtttc agggggcatt gtcgcctggt cgggtggcgt taatctggcg
     7741 aaccgtcttt ccgaaacgtt tgctgtgatg aatgcgccag gaattaaaga tgcttatgtc
     7801 aatgggcaaa aatatcgcac aacaaaccgt aatggagtgg tggtatacga cggaatgaca
     7861 ccttatcggg aaaatcacct gatgctgggt gtgtcgcaaa gcgatagcga agcagaatta
     7921 cgtggcaacc ggaaaattgc cgccccttat cgcggcgcgg ttgtactggt taattttgat
     7981 accgatcagc gcaagccctg gtttataaaa gcgttaagag cggatggtca accattaacg
     8041 tttggttatg aagtcaatga tatccatggt cataatattg gcgttgtcgg ccagggaagc
     8101 cagttattta ttcgcaccaa tgaaataccg ccatcggtta atgtagcaat tgataagcaa
     8161 caaggacttt catgcacaat caccttcggt aaagagattg atgaaagtag aaattatatt
     8221 tgccagtaat agtcaggtgg ttctatggaa attcgcataa tgctatttat attaatgatg
     8281 atggttatgc ctgtgagcta tgcggcatgt tatagtgagt tatctgttca gcacaatttg
     8341 gttgttcagg ggaattttgc acttactcaa acacaaatag cgacatatga gcataatttt
     8401 aatgattcgt catgcgtgag tacaaatact atcaccccta tgagcccatc ggatattatt
     8461 gttggacttt ataacgatac cattaaatta aatttacatt ttgaatggac caataaaaac
     8521 aacatcacgt tgtcaaataa tcagagcagt ttcaccagtg gttattcggt aacggtgaca
     8581 cctgcggcca gtaatgcaaa agtgaatatt tctgcggggg gcggtggttc agtgatgatt
     8641 aatggtgttg cgacattatc cagtgcttca tcatcgacac gcgggagtgc cgcagtacaa
     8701 tttctactgt gtttattagg tggcaagtca tgggatgcat gtgtaaatag ctacagaaat
     8761 gcattggcac aaaatgcagg tgtctattcc ttcaatctga cattgtcata caacccgata
     8821 accacaacct gcaaacagga cgatttatta attactttag acagtattcc cgtttcacaa
     8881 ttaccagcca caggtaacaa agcaacaata aatagtaaaa aaagggatat tattatgcgt
     8941 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     9001 agttctgact tgttaaccaa cagcgacaca atactgaaag gtgcggaaga taatgcgtag
     9061 gatttattct tgaaagtaat ggttcgccag tcacactttt aaatatcact aacagcagta
     9121 aaggatacac aaatttaaaa gaaattgcgg caaagtcaaa acttacagat acaacggttt
     9181 caattccgat aacagccagt tactacgtct acgatacaaa caaagttaaa tctggcgcac
     9241 tggaggcaac cgcattaatc aacgtgaaat acgactaata acaagcaaga cgagcaagtg
     9301 gctaataata aaaaataaca aggtgtctgc atttccccct catgatgagg gggctttttt
     9361 tagcgatgat aaaaaatctc accgtcgtag gctttaatga ttttaccgtc ggtgtcgctg
     9421 atcaacacgt acgcgccacc catataggtc cagtgcgtcc cggcatcagg cgcgggcagg
     9481 ttacgtaact gccactgctt aatgttgtac tcatcggtgc ggtacatctc tgggacggta
     9541 tcacctggtt tgaaatgctt aaagtcagca atgaaactac ttaactcata cttagaaatt
     9601 cccgaggtat tggtcgaacc cgcggtggct ggtgcggccc aggcggcact ggtaaccagc
     9661 aaaagcacac ccagcaacat cttattttta atggtcataa actctccact ttggggatct
     9721 gtaatatcag tcgcatatct tccctgccat acaatataac tgacaagcga tattttgtta
     9781 gtgatcccat gatttcgtaa aaagtgtaaa tgaaaggatg ccctaaaagc atccttagcg
     9841 agcagagagt cagcggaaat agctgattta agaatcatcc tgggctgaag ataacatgca
     9901 agcctaaagg attgagttat cgcattatgc ccatgaagcc atgtacgccc atatacacac
     9961 cgactaagcc aatcaacaga ctggaaaaat agggggcgcg tttagcgaga gtgttaaatc
    10021 cgctccagcg ttttgcgacc tgctgaacgc tgattgctgc gccaacgcct acggtgacaa
    10081 gcgttaacgc caggccaatg ctgaaactga cgaccagtgt tgcgcccagt gtcagggctt
    10141 tcaactgaat gcatatcaac agcacggtaa ttgttgccgg gcaggggata aggccaccgg
    10201 ttaagccaaa taacaaaatt tgccagttga tgacctctct accatcaaag cgtcgtttaa
    10261 tgtcattggc atgggctcgt gcatgggcat cctgatactc gccatgttcg tgatggtgat
    10321 gatgtccttg gtcgtggtga tgttcgtggt cttgatgatg ttcgtgatcg tgatgatggt
    10381 gatgatgctc ataatcatgc ccgtgcatat tctccagcca gttgcgttcg ccgcgccagg
    10441 tacgccagaa catccagaac acggtgccaa taatgatcac tgcggaaatc agctggagcc
    10501 acggttctgc tgattgagca gtaaagcgct tactgatcac catcccgcca aaggcaatca
    10561 accagaccac cgcggtatgc gaaatggttg ctgccagtcc gagcatcacc gcctgtttaa
    10621 tggtgccttt gatggcgatg ataaacgccg ccatcatcgt ttttgagtgc cctggttcca
    10681 ggccatgaag cgcaccaagt aagatggcgc tggggatgaa gaaccaggcg tttccttgct
    10741 gaagaagagt tgtaaattcg gtcatgataa taattcttag tattaattcg gcaatctgat
    10801 tctactcccc cccagtacct gatactaccc cccagtagat taatagtgct atgatttttc
    10861 atattcttgt taaccaggtg ttgccatgtc tcatacaatc cgtgataaac agaaactgaa
    10921 agcgcgtgcc agtaagattc agggccaggt cgtggcgctc aagaaaatgc tcgacgagcc
    10981 gcacgaatgc gctgcagttt tacaacagat tgctgctatc cgtggagcgg taaacggtct
    11041 gatgcgggaa gtgattaaag gtcatctgac ggaacacatc gttcaccagg gggatgagct
    11101 aaaacgtgaa gaagatctgg atgtcgttct gaaggtgctg gattcatata tcaaataatt
    11161 tattaacgcg attgtaaaac tgccgttttt cctcgtttac aacgggtgcg ctggacatta
    11221 ccatcctcct ctgcgattta tcatcgcaac caaacgactc ggggtgccct tctgcgtgaa
    11281 ggctgagaaa tacccgtatc acctgatctg gataatgcca gcgtagggaa gtcacggacc
    11341 accaggtcat tgcttcttca cgttatggca ggagcaaact atgcaagtcg acctgctgag
    11401 ttcagcgcaa tctgcgcacg cgttacacct ttttcaccaa cattcccctc ttgtgcactg
    11461 catgaccaat gatgtggtgc aaacctttac cgccaatacc ttgctggcgc tcggtgcatc
    11521 gccagcgatg gttatcgaaa ccgaagaggc cagtcagttt gcggctatcg ccagtgcctt
    11581 gttgattaac gttggcacac tgacgcagcc acgcgcacag gcgatgcgtg ctgccgttga
    11641 gcaagcaaaa agctctcaaa cacgctggac gcttgatcca gtagcggttg gtgcgctcga
    11701 ttatcgccgc catttttgtc atgaactttt atcttttaaa ccggcagcga tacgtggtaa
    11761 tgcttcggaa atcatggcat tagctggcat tgctaatggc ggacggggag tggataccac
    11821 tgacgccgca gctaacgcga tacccgctgc acaaacactg gcacgggaaa ctggcgcaat
    11881 cgtcgtggtc actggcgagg tggattatgt taccgatgga catcgtatca ttggtattca
    11941 cggtggcgat ccgttaatga ccaaagtggt aggaactggc tgtgcattat cggcggttgt
    12001 cgctgcctgc tgtgcgttac caggcgatac gctggaaaat gtcgcatctg cctgtcactg
    12061 gatgaaacaa gccggagaac gcgcagtcgc cagaagcgag gggccaggca gttttgttcc
    12121 acatttcctt gatgcgctct ggcaattgac gcaggaggtg caggcatgaa acgaattaac
    12181 gctctgacga ttgccggtac tgatccgagt ggtggtgcgg ggattctggc cgatcttaaa
    12241 accttctcgg cacttggcgc ttatggttgc tcagttatta ctgcactggt ggcgcaaaat
    12301 acccgtggcg tacagtcggt gtatcgcatt gagcctgatt ttgtcgccgc ccagctcgat
    12361 tcggtgttca gcgatgtgcg aatcgatacc actaaaatcg gtatgttggc ggaaaccgat
    12421 attgttgaag cggtggcaga acggttgcaa cgttatcaga tccaaaacgt ggtactcgac
    12481 accgttatgc tggcaaaaag cggcgacccg ctgctttcac cttcggcggt tgctacgctg
    12541 cgcagtcgat tattgccaca ggtttcatta ataacgccaa acttgcccga agctgccgcc
    12601 ttgctcgacg cgccacacgc gcgcaccgaa caggaaatgc tggaacaagg gcgatcgctg
    12661 ttggcgatgg gctgtggcgc agtgctaatg aaaggtggtc atctggatga tgagcaaagc
    12721 ccggactggc tgtttacccg cgagggtgaa caacggttta ccgcaccgcg cattatgacc
    12781 aaaaacaccc acggcactgg ttgtacactc tctgcggcgt tggctgcact acgcccgcgc
    12841 catacaaact gggctgacac cgtacaggag gcaaaaagct ggctttcatc ggcgttagcc
    12901 caggccgaca cgctggaagt tggtcacggt attggtccgg ttcaccactt ccacgcctgg
    12961 tggtgagcac cgtactatag ctgctttggt tattgtcagc ctttggcact gacattcagg
    13021 gatgaaacca atgcagctaa gaattaccag tcgaaaaaaa ctcaccgctc tattatgcgc
    13081 tcttgggcta atttccatcg ttgcaattta tccgcgtcag acggtgaatt ttttctattc
    13141 gacagcagtt cagattacag actacatcca cttctacggt tatcgcccgg ttaaatcttt
    13201 cgctattcgc attcctgcca gttacaccat tcacggatta gatgtttcgc gctggcagga
    13261 gcgtatcgac tggcagcggg tggcaaaaat gcgcgacaac gatatccgct tacagtttgc
    13321 ttttattaag gctacggaag gcgaaaagct ggtggacccc tatttttcgc gtaactggca
    13381 attaagccgc gaaaatggcc tgctgcgcgg ggcgtatcat tatttctccc cgtcggtatc
    13441 cgcttcagtt caggcgcgat tatttctgca aacggtggat ttctcacaag gcgatttccc
    13501 tgccgtgctg gacgtagaag aacggggaaa attatcggca aaagaattac gcaagcgggt
    13561 aagtcagtgg ctaaaaatgg tcgaaaaacg cacgggaaaa aagccgatta tttactcagg
    13621 agccgttttt tatcacacca atctggcggg ctatttcaat gaatatccgt ggtgggtggc
    13681 tcactattat caacgtcgtc cggacaatga cggcatggcc tggcgcttct ggcagcattc
    13741 cgaccgtgga caggtagatg gcatcaatgg tccggtggat tttaatgtgt ttaatggcac
    13801 ggtggatgag ttgcaggcat tcgttgatgg gattaaagaa acgccttaaa taagaagcag
    13861 tgatatactc ccaccagact ccctggcagg aaaaagacaa atggaacaag cgcataccca
    13921 gctgattgcc caactgaacg agcgtatttt agcggcggat aacacgccgc tttatattaa
    13981 gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg ctggagcatg gcaatatttt
    14041 gcccggtgag cgtgattcaa gtcagttaac cggcgtttcg cgcattacgg tgcgcaaggc
    14101 gatgcaggcg ctggaagaag agggcgtggt gacgcgttcg cgcggttacg gcacgcagat
    14161 caacaacatc ttcgaatact cgttgaaaga agcgagaggt ttttctcagc aggtggtatt
    14221 gcgcgggaaa aagcccgata cgctatgggt taataagcgt gtcgtgaaat gtcccgaaga
    14281 agttgcgcag cagctggcgg tcgaagcggg aagtgatgtc tttttgctta agcgtattcg
    14341 ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg gttccggcgc atttaattca
    14401 tgatgttgat gccatcggaa tttctcttta tgactatttc cgcagccagc acatttaccc
    14461 acaacgtacg cgttcccgcg ttagcgcccg gatgccggat gccgagtttc agtcacatat
    14521 tcagttagat agcaaaatac cggtgctggt gatcaagcaa gtggcgcttg accaacagca
    14581 acggcctatt gagtacagca ttagccactg tcgcagcgat ctatacgttt ttgtgtgcga
    14641 ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg atgcccgaca acccacgacg
    14701 ccactgcatt gcccagtaat acggcatccg ccagtggcag accagaggcc agcccggcaa
    14761 gtacgccacc tgcatgactg tcgcccgccc caatggtgtc tacaacttgc gttggaaatg
    14821 cccgaatgca gccagaagcg tcgttgctga aataccatgc gccttcttta tcgagacgaa
    14881 cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc aagtgttgtt atctctgcag
    14941 ataaagcaaa acgttcggcg gcaatctcag cctcttgacg attgagtgac actaaaggtc
    15001 gacaggccat gatccgcgcc agtaatgcgt ccgggatatc gccaatacgt gggccaaaat
    15061 cgataaacgg cgtcacgtct tgcagctttt ccagccattc cactaacaat tcgccgcagg
    15121 gcgaggccag ttgataaccg gaaaaataga gcaggttgcc aggcgcaacg gttaatcgtg
    15181 ccaaccattg gcgattccat tgattttcca caccgctgaa tgacataaaa gtgcgttcgc
    15241 catccggctc aaccagtgcc agacaccagc cgttatcgct ttcggcgtta tcgatcaggc
    15301 tgattaaccc ctctttcgcc atccggttgc gaataatctc cgcccacacg ccctgaccga
    15361 gcggcaaggc attacccgct tcgatgccga ggcgctttaa cgccacggca atattcagtg
    15421 cgcagccgcc aacgttaacg ctctgctgtt tcagttcgat atcgcaccca cgccagggga
    15481 gggcataagc gtcggcgatc acgtcaatga ccgccgcgcc gacgaccatc accggctgac
    15541 gcgtggttaa ttcaggcagc agtgtgtgta atcgagcgcc gctcataccg cctcccgttg
    15601 ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa tcaagctgat ttaccgcatc
    15661 cagctccgcc tttaacgcag gatcgacagc gttaatgcca tgcaacgcgc cgcaaatcgc
    15721 cgtcgccata gcacctatgg tgtctgtgtc gccgccaaga ttggcgcaca ggacggcgca
    15781 gcggtttggg tcggtttgtg ccagttcaac tagcgcaatg gcgcacggaa cggactcaat
    15841 agtgctggtg cctgcgccaa cgacctggta aagctgttcg ctggcggatt cggtgccgtc
    15901 ggcattacgc acaattttca gcgcaatctc cagacgtgcc gccagtgagg cgctgaacgt
    15961 ggtggtgcgt ttttgctgtg catgtcgcgc aattgaaggc aatgaatcaa caatcgctga
    16021 ccagcgttct ccgtcaatgg cacgagaaat cgcccatgcg atgactaccg cgcctgcaac
    16081 cgccagatcg gatttatggg tcgggctgga cgccagcgcc acatcatcaa taaaggaatc
    16141 aacatcacgc gctggaagca aacaacctaa tggcgagacg cgcatcgctg cgccgttggt
    16201 tacgccgttg ttttccagtt cagcaacggg tttaccgtcg cgaatggcgt taagcgcaat
    16261 cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg aaacgcaacg cccagtcgag
    16321 aatattacgc ccaatcagat tcggatcgat cttgccttca cattccagta acgcatccgc
    16381 cagacacagg ccatcgaggt atcgtcggtg aattcggcgc ggttaaaata acaggccgcg
    16441 ttattctcct ttggtccagg cagaaaacgg tcaatccagc caaagtgtgc tttaacgcgg
    16501 ctgcgtggcc aaagctcgga gggcatcccc atcgcatccc ctaacgcctg cccataaaga
    16561 gcaccgagaa tacgttctgt tttcatttaa cttccccttg tgtcaacgca atatctcgat
    16621 catcgacctt gatagccgta atttcgttgt cggattcgcg gaaaaaaatc atgaacagca
    16681 cggcgataat ggcaatcatc accgcgccga aagtccacat cccggaccag ttgaaagtca
    16741 gtccgtttac cggttcctga taagcgaaca tcttttccat catcacaccg ccaagacgat
    16801 agccgagcaa actgccgaag ccctggcagc agagcgtgat cagcccctgc gcagcggtac
    16861 gcatatgcac gggggctttt ttatcgacat agatgtaagc ggtaacgtag taaaaatcgt
    16921 aacttacgcc gtgaagcaaa ataccgagga acagtaacgc gtaggtgaaa ttgacaggta
    16981 atgtaaggta cgcgatgaca ataccacata ataatacctt ttgaatacct ttgcggtgaa
    17041 aacttgaccg cgctaacatt ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac
    17101 atccaaaaga tggaaaaact cgcccgtaac gaccaggatt atgattgccc gcaaaaggcg
    17161 ggccaggaca atccatagcc gatatccaat cggaatttac gggagcatag taatgacaga
    17221 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgactat
    17281 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtga tgattgacaa
    17341 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    17401 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    17461 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    17521 agcgggctgt aactgtgtgg catcaactta cggcgtgttg gtgtcggtat cgcggcgcta
    17581 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    17641 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    17701 ggttggtgcg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    17761 tgcggccttt gaacgcgcgc acgagctggg catggtggca gtgctgtggg cctatttgcg
    17821 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgactggtca
    17881 ggcgaaccat ctggcggcaa ccataggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    17941 taacggcggc tataaagcga ttaattacgg ttataccgac gatcgcgtgt acagcaagtt
    18001 aaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    18061 ggctgggttg ataaactccg gcggtgctgc aggcggtgaa actgacctca gcgatgcagt
    18121 gcgtactgcg gttatcaaca aacgcgcagg cggaatgggg ctgattcttg gacgtaaggc
    18181 gttcaagaaa tcgatggctg acggcgtgaa actgattaat gccgtgcagg acgtttatct
    18241 cgatagcaaa attactattg cctgatacgc ttcatacgaa cagcaccacc ccgtaggacg
    18301 gataaggcgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    18361 catgcctaca tagcactgcc acgtatgttt acaccgcatc cggcataaaa acacgcgcac
    18421 tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc gaacaatttt
    18481 catattgtcg tttttgtgat cgttatctcg acatttaaaa gcaaataatt tcattatatt
    18541 ttgaaatcga aagcaaacga caagatatga aaatgtacgt ggtatcgaca aagcagatgc
    18601 tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac aatctcgaaa
    18661 cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc atcgccggaa
    18721 cgcctggcac atttactcat gctggtacag aaaatctgtt ggcgctggtc agcgcgatgg
    18781 cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa tttgacgata
    18841 tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt
    18901 ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc tttgatgtca
    18961 gcgtcgaggc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag
    19021 ccgatgcgtt gtacaccaac cccgcacagg cgcgtgaatt tgccgaggca accgggattg
    19081 attccctggc ggtcgccatc ggcacggctc acggtatgta tgccagcgca ccggtgcttg
    19141 atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg ctgcatggcg
    19201 cgtcaggatt atcgactaag gatattcagc aaaccatcaa actggggata tgcaaaatca
    19261 acgttgcaac ggagctgaaa aatgccttct cgcagtcgtt aaaaaattac ctgaccgagc
    19321 accccgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca atgcgcgatg
    19381 tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc acctgccatt
    19441 taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga acatatcggc
    19501 atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc atttgatcgc
    19561 aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca atttggcggt
    19621 tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc cgataaagtt
    19681 gggtttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa ctgctggcag
    19741 caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt
    19801 gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga tcccataccg
    19861 ttagcaccag aaacggttgc ggaacgagct gctgtgcttt gctttgctgc ggaaagtgtg
    19921 gcgacagatt gccagcgtga gcaactgagc tatgtcattg gcactgaagt tccggttccg
    19981 ggcggtgagg ccagcgccat tcagtcagta cacatcaccc atgttgaaga tgccgccaat
    20041 actttacgta cgcatcaaaa ggcctttatt gcccgtgggc tgacagaggc gttaacacgt
    20101 gtgattgcca tcgtggtgca gccgggtgtg gaatttgatc acagcaatat tatccattat
    20161 cagccgcagg aagcgcaggc gctggcgcaa tggatagaaa acacccgaat ggtttatgaa
    20221 gcacattcta ccgattacca gacccggacg gcttattggg aattagtccg cgatcacttt
    20281 gcaatattga aagtcggtcc cgcattaacc tttgctttac gcgaggcgat atttgcactg
    20341 gcacaaattg agcaggaact tatcgccccc gaaaatcgca gcggttgcct ggcggtaatt
    20401 gaagaagtga tgctcgacga accgcaatac tggaaaaaat attatcgcac gggttttaac
    20461 aattcattac tggatattcg ttacagtctg tcggatcgta ttcgttatta ctggccgcat
    20521 agtcggatta aaaatagcgt tgaaacgatg atggtgaatc tgcaaggcgt ggacatccca
    20581 ctgggcatga ttagtcagta tcttcccaaa caatttgaac gcattcagtc cggggaatta
    20641 tcagcaatgc cgcatcagct tattatgaat aaaatttatg atgttttgcg cgcctatcgc
    20701 tacggctgtg cggaataagg acggtatatg actaacctgt ttgttcgtag cggaatttct
    20761 tttgtcgatc gtagcgaagt tttaacctat atcggtaatg agatgctcgc gaaaggtgtg
    20821 gttcatgata cctggccaca ggcattaatt gccagagaag cagaattccc taccgggata
    20881 atgcttgagc agcacgccat tgcaataccg cattgtgagg cgattcatgc taagtcgtca
    20941 gccatttatc tgttaaggcc aacaaataaa gttcattttc agcaagcgga tgatgataac
    21001 gacgtggcgg tatcgttggt tattgcgttg attgtggaaa atccgcagca gcaattgaaa
    21061 cttttacgct gtttatttgg caagttacaa cagcccgata tcgtcgagac actaattact
    21121 cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg ttttagattc agacgaataa
    21181 atccctctgt aacaataaaa aggactattt atgaaacgca agattattgt cgcttgcgga
    21241 ggcgcggttg cgacctctac gatggcggcg gaagaaatta aagagttgtg tcagagtcat
    21301 aatattcctg ttagtgtcgg gttaatgaaa tagaaaccta tatggatggt gtgcatttga
    21361 tatgtaccac tgccagggtg gatcgtagtt ttggcgatat tccgttagtt cacggcatgc
    21421 cttttgtttc tggtgtcggt atcgaagcat tacaaaataa aattctgact atcttacagg
    21481 ggtgacctat gttttcagaa gtcatgcgtt atattctcga cctcggccct acagtgatgc
    21541 tgccggttgt catcattatt ttttctaaaa tattaggcat gaaggcaggc gattgcttta
    21601 aagcgggtct gcatatcggg attggctttg ttggcattgg ccttgtgatt ggcttaatgc
    21661 tggattccat tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg aatctgcatg
    21721 tggtcgatgt cggctggccg ggctcttcac caatgacctg ggcgtcgcaa attgcgctgg
    21781 tggcgattcc gattgcgata ttggttaacg tggcgatgtt actgacccgt atgacgcggg
    21841 tggtaaatgt tgatatctgg aatatctggc atatgacctt caccggcagc gttgctgcac
    21901 ctggcaaccg gttcatggat gatagggatg gcgggtgtgg taattcacgc ggcgtttgtt
    21961 tataagctcg gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggtatt
    22021 gctattccgc acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc
    22081 atcgagaaaa tcccaggcgt taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa
    22141 tttggccctt ttggcgaacc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc
    22201 ctcgcgggtt acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg
    22261 ctgctgatgc cacggatgat taaacccatc atggatggtt taacgcccat cgctaagcag
    22321 gcccgtagtc gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc
    22381 gcattactgc tggggcatac ggcggtggta tcggcaagcc tgatttttat tccgctcacc
    22441 attttaattg ctgtttgtgt tccgggtaat caggtactgc cgtttggcga tcttgccact
    22501 atcggcttct tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta
    22561 atctcgggtg tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg
    22621 cacacccaac tggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg
    22681 gatcagggcg gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt
    22741 cccggtttca ttattatcgg cgcaatttat ctgaccggta ttttcatgac ctggcgtaga
    22801 gcgcgtggtt ttactaaaca agagaaagcc gttctcgcag aataattttt acctgagggg
    22861 gaggttatcc cccttaacaa tcaggagttt ttatgaaatc agtagtgaat gatactgatg
    22921 gtatcgtgcg cgttgcaaaa agcgtcattc ctgaaattaa acatcaggat gaggtgcggg
    22981 taaaaattgc cagctcgggc ttatgtggtt ccgatttacc caggatattt aaaaatggtg
    23041 cacattatta tccaataacg ttaggccatg aatttagcgg ctatattgat gcggtgggat
    23101 ccggtgttga tgatttacac cctggcgatg cggttgcctg tgtgccgtta ttaccctgtt
    23161 ttacttgtcc agagtgtttg aaagggtttt attcccagtg cgcaaaatat gattttattg
    23221 gctcgcggcg tgatggtgga tttgctgaat atattgtcgt taagcgaaaa aatgtctttg
    23281 ctctacccac ggatatgcct attgaggatg gggcttttat tgagccgatt accgttggcc
    23341 tgcatgcttt tcatttagcg caaggttgtg agaataaaaa cgttattatt attggtgccg
    23401 gaaccattgg cctgctggct attcagtgcg ctgtcgcgct gggagcaaag agtgtgacgg
    23461 cgatcgacat tagctcagaa aaactggcac tggcaaaatc tttcggtgcg atgcaaacat
    23521 ttaacagtag cgaaatgagc gcgccgcaaa tgcagagcgt tttacgcgaa ctgcgcttta
    23581 atcagcttat cctcgagacg gctggcgtac cgcaaactgt cgaactggcg gtagagattg
    23641 ccggtcctca tgcccaactg gcgctggtgg gcacgttgca tcaggatctg catttaacat
    23701 cggcaacgtt tggcaaaata ttgcgtaaag agctgacggt tatcggcagt tggatgaact
    23761 attccagccc ttggccgggg caggagtggg aaacggcgag ccggttgctg acagaacgta
    23821 agttaagcct ggatccatta atcgctcacc gtggaagctt tgaaagcttc acccaggcgg
    23881 tgcgtgacat cgctcgtaat gccatgccgg gcaaagtgtt gctcattcca tgaaaccgcg
    23941 ggccagcgtg atgctggccc ggtattgtgc aaaacagatc attcaccaat ggtccccctt
    24001 cgtttacact agccacaatt gaatatgggt aaatgacgat gaattcattc gagcgaagga
    24061 ataagatcat ccaattagtg aatgaacagg gaaccgtgct tgttcaggat ctggcgggag
    24121 tatttgctgc ctcggaagcg acaatccgtg ccgatttgcg ctttctcgaa caaaaaggcg
    24181 tggttacgcg ctttcatggc ggtgcggcga aaataatgtc cggtaatagt gaaaccgaga
    24241 cccaggaagt cgggtttaaa gagcgatttc agctcgccag cgcgccaaaa aacagaatag
    24301 cgcaggcggc agtcaaaatg atccatgaag ggatgaccgt tattctcgac agcggaagta
    24361 ccacaatgct tatcgccgaa ggattaatga ccgctaaaaa tatcacggtg attaccaaca
    24421 gtctcccggc agcgtttgcc ctttccgaaa ataaagacat tactctggtc gtctgtggtg
    24481 gcacggtccg ccataaaacg cgctcgatgc atggttccat tgccgagcgt tcattgcagg
    24541 atattaatgc cgatttaatg tttgtcggtg ctgatggtat tgatgcggtt aatggtatta
    24601 cgacctttaa tgaaggttat tcgattagcg gggcgatggt caccgccgca aataaagtga
    24661 ttgccgttct cgattcatcg aaatttaatc gtcgcggttt taatcaggta ctgccaatcg
    24721 aaaaaattga cattattgtt accgatgatg ctgtgtcaga ggtggataaa ttggcattgc
    24781 agaagacacg ggtaaaatta attacggtgt agtattgttc tgtatgccgg ataaggtgtt
    24841 tacaccgcat ccggcattac ggattctccc tggttcaaga gagaatagtt tgattaacgc
    24901 aataatggac aatctggtgg taatcgacaa cgcaacgccg ccggaagtat ttcaatatgg
    24961 aagttttgcc cactcaacgg ttcgccatca agattaaaag tgatttcgtg tggggcttgt
    25021 atatcaaacc acgacgaagc gccttcgata atattcgggt tatcttcgtc agattttaag
    25081 gttgatacga gagccggaag aatttcatcg ccggtaaaaa tgcgcagttg cagcaagcca
    25141 tcgttaatta acgcgttcgg gcacaattgt tgaccgccac cggcctgacg cccgttacca
    25201 ataccaatga ccagggcgtc accttgccag tgaaagtttt caccgcggat ttcacaacgg
    25261 tccggttgca gagtatccat gcgcattaag ccatgaatga tgtaagagac gccacccagc
    25321 gcggctttta atttttccgg cgtttctgtg gtaatacgcg tcccaaatcc gcctgtcgcc
    25381 atattaataa aacaggtttg tttgttgacc tgcgccatat ctatcgcaat ggcattaccg
    25441 gcaattgcca gtttcagcgc cttatccagt gcctcaggaa tccctacact ggtggcaaaa
    25501 tcattggcgg ttcctaatgg caaaattccc agcgcgggta tgtcatcccc ctcacactga
    25561 atcaacgccg tagaaacttc attaatggtg ccatcgccac caccggcaat caccgttgcg
    25621 acgcccaact tccgggcctc ctctacaaat cgtgcggcat cacctttctc ccaggtgacc
    25681 cgcacatgga tcgtcattcc ttcctcacgc aacagcataa ttgcttcgcg caagggtaga
    25741 ttgtcagtac ttttgccatt aagaatcagt aagctggcgg gaaattctgc catgatcgta
    25801 tgtgccttta tgattggtct gtagatagtg tagagcagaa aacaaaaggt ggagtcagaa
    25861 caggatgaaa gtcggaggat aaaaagtcag ttcaagtatg gaggattcag tggttaccac
    25921 caggagcttg caatggggac agcgaatact aacggctgcc accgataaat ttcaaaaaag
    25981 agcatatacc taatattcaa ctaaacagtg gcatcttcaa tataatatat taaagccccc
    26041 atggagttac cctgaagggc ctcaatgtcc gtaattccta cttatgtagg aaatgttgta
    26101 cagaacattt attataatcc tattcaatta taataatcat gccattatta tatttaaaca
    26161 ctagagagtg tcgttggtat ttaatggggg aaggtgagat gaaaaagata gctgctatat
    26221 cattaattag tatttttctt atatctggtt gtgctgtgca taatgatgaa acaagtatcg
    26281 gtaaatttgg tcttgcatat aaaagtaata ttcagcgtaa actcgataac caatactaca
    26341 ccgaagccga agcttcttta gccaggggca gaatatctgg tgcagaaaat atagtaaaaa
    26401 atgatgcagc ccatttctgt gttactcagg gcaaaaaaat gcagatagtt gacctgaaga
    26461 cagaaggtgc aggattacat ggcgtcgctc gtctgacatt caaatgtgga gagtgagaat
    26521 attttttggt aagcgtcaaa catgcgcgtt ctggctgtgc gtagccggaa cctgggcgag
    26581 cacgataccg ttacgtgaaa ggcattgtgc tatgaagggg gattctatcg atgtggtcaa
    26641 tggaagacgg ttatcaggga tcaatccttc tttacagctt atgtacaaaa aataagcccg
    26701 tgtaagggag attacacagg ctaaggaggt ggttcctggt acagctagca ttttatggat
    26761 tatgtttttc agcgaaacgg atgataacct taataaatgc agctgtatgt gatcggtttc
    26821 taagaatttt ccatccggga aaaataatcg aaattaatca cttaccgtgg gggttacgcg
    26881 tggtttcccc ggagaaatta cgcattagca gcgcgtaatt tagctcaaga tcctgcggga
    26941 ccgggagcca cacagtataa ccatcgcctg gtgcgaccgg catagcttcg cctttggcgt
    27001 tttccatgtg ctcaagggta aagttaatgt tgccttgcgg cgtcatcagc tcaagactgt
    27061 cgccaacgga gaatttattt ttcaccgcta ccgctgcgag gtcccccttg cgctcaccgg
    27121 taaactcacc aacaaactgc tggcggtcag aaactgaata accgtattcg tagttctgat
    27181 aatcgtcgtg agtatgacga cgcaggaaac cttcggtata gccacgatgc gccagacctt
    27241 ccagagtttc cagcaggctg gtatcgaacg gttttcccgc agcggcgtca tcgatagctt
    27301 tgcggtaaac ctgtgcggtg cgtgcgcaat agtagaaaga tttggtacga ccttcgattt
    27361 tcagcgaatg cacgcccatt ttggtcaggc gttctacatg ggcgatggcg cgcagatctt
    27421 tcgagttcat gatgtaagtg ccgtgctcat cttcaaacgc ggtcatatac tcgcccggac
    27481 gctgggcttc ttcgatcata aacactttgt cggttggcgc gccgataccc agcgtcggct
    27541 caacattttg caccggaatc ggctcgtact tgtgtacgat gttgccaacg tcatcttctt
    27601 tcccttcctg gacgttgtac tcccagcggc aggcgttggt gcaggtgccc tggttcgggt
    27661 cgcgcttgtt gatatagcca gagagcaggc agcgaccgga gtaggccatg cacagtgcgc
    27721 cgtgaacgaa gatctcgatc tccatatccg gcacctgatt atggatctct tcaatctctt
    27781 ccagcgacag ctcgcgagag aggatcacgc gggtcagtcc catttgctgc cagaatttca
    27841 ccgtcgccca gttcacggcg ttagcctgca ccgagaggtg gatcggcatt tctgggaagt
    27901 gctcacgcac cagcataatc agccctggat cggacataat cagcgcatcc ggccccattt
    27961 ccaccaccgg tttcaggtca cggataaagg ttttcagctt ggcgttgtgt ggtgcaatgt
    28021 tgaccacgac ataaaacttt ttccccagcg cgtgggcttc attgatgccg agctgaagat
    28081 tttcgtggtt gaattcgttg ttgcgcacac gtagggagta acgcggctgg cccgcataaa
    28141 cagcatctgc gccataagcg aaagcgtaac gcatattttt cagcgttccc gccggggaaa
    28201 ggagttccgg tttaaacata attctctcgt tctgatgaca ggtcagatcc gcttcacctg
    28261 atgaagcggt taggggaggc ccccacttta agggcgggca ttgtagcgct gtagggggga
    28321 tctggtaaag cgccggacgc caacaaccca cgcccggcat cgtaattacg tattgtcttt
    28381 caccgtctgg cttgtgccat tggctttaac ggaggcaatt ccggtttcac gagattgcgc
    28441 ggtggcgtac atctggctgg agccgataat ttgatgatta gcggctttca gattgaaata
    28501 gaatttgccg ttgcttgccg tttttttctc atagcgctct tcttgcgggc tgttgctacg
    28561 caccgacgcg atgccctttt ccgcagaggc ttttgaggta tacagctcgc tggtgaggat
    28621 agtctcacca ttgcccgctt ttagcacaaa ccggaactga ttatcactgc tcttactgag
    28681 ttcaaaccaa ccagccataa tgactccttg attgaaattg cttatcgaaa acagcatcta
    28741 aaggtgctgt ttactgatta aagtatgagc gattttctga tgcctgccag ggcggagaga
    28801 taatgtcagt ttgctgacca tgcgtggaat aatgttgccg gatcgggtct gaacgccatc
    28861 tccggctaac aaagtaatgt cgctaaaact aaacgatgcg gcaggcgtcg gcttcccagc
    28921 ggtaaccaac gccgtaaacg gcgcggataa atgactgttc ggcgtcgaga gattccagct
    28981 tgcggcgcag atttttaatg tggctgtcaa tggtgcggtc ggttactacg cggtagtcgt
    29041 cataaagatg attgagcaat tgctcgcggg agaacacttt tcctggttcg tgagagagcg
    29101 ttttaagcag acgaaattcc gcaggcgtca ggtcaagcat tttaccgcgc catgaagcct
    29161 gaaaacgacc ttcgtcgata atcaacgggc tttcggcatc ctgttgctga agttcgcgtt
    29221 gcggtttaca acggcgcaaa atggttttga cacgcgctac cacttcccgt gggctgtacg
    29281 gcttgcagat gtagtcatcg gcgccaatct ccagccccag caggcggtcg atctcttcga
    29341 tttttgccgt caccatcacg atcggaacat cggaaaaacg acgaatttcc cggcataatg
    29401 tcaggccatc ggtgccaggg agcatcagat ccaacaggat cagatccggt ggtgtctggc
    29461 gcacatacgc cagtacctga tcgccgtggc tgataagcgt cggcgcatag ctcgcagcac
    29521 gcagataatc aatgagcaac tgccccagct tcggttcatc ttccacgatc aaaatacgcg
    29581 gtgtgttttc gtcgattggt aactcggtca tacttctctc tgtaaatccc gttccagcgg
    29641 taactctact gtaatgctta ccccgccaaa aggcgaatgg gcggcaataa tgcggccatt
    29701 atgggcttca acaatgttca ggcaaatcgc cagccccagc ccggaaccgc cgctggcgcg
    29761 gttgcgggaa ccttcggtgc gataaaaacg ttcaaacaat ttttgtagct gatcgtcact
    29821 gacacctggc gcactgtcgg caaaggtcag gcgcaccgtt ttgtcgtgct gctcggcaga
    29881 gattttcagg ctgccgccgc tgtcagtgta gcgcaggctg ttttccagta agttattgaa
    29941 taactgcatt aaacggtcgc gatcgccaaa tacggtgata ctgtctggca gggaaaattg
    30001 cagtttcagg ccacggctgg cgaatcgttc gcgaaatgcg ccgcccgcca cttccagcag
    30061 tgggatcaaa tctaccggtg ctttttgata ggcgagagcg ccttcatcag acatcgacaa
    30121 ctgatgaaga tcgtcaacca gtttggtcag tgtaccgacc tccgcctgta aagacgccac
    30181 cgtctccggc gtgaatttgc gcacgccatc ctgaatggct tccagttcac cgcgcagcac
    30241 cgctaatggc gtacgcagtt cgtgagaaat atcggccata aaatcgcgcc gcatttgctg
    30301 gtttttctcc agcgtgctgg cgagctggtt gaagtcttgc gccagtttgc ccagttcatc
    30361 ttcactggtg ggcgttacgc gggtagtgaa atcgcccgcc gccagtttgt gcgtgccatc
    30421 gataagtcgt tttaccggtg ccagcaaacc gcgcgccagc agaaaggtgg cgagcgccgc
    30481 gagtaacgtt gctagggcga cgatcagcca gctggtttgc cgctgttgtt tatcgaaatt
    30541 gatatcagta ttgcgggtca gccgctcaac aggggaggca atcaccgcgc ccacttccgc
    30601 accgttgacc agaatgggtc gccgtgtacc gtcaggtgga atcggcgctc gcggaccaac
    30661 cagtactttg ttgttttgat cgaccaccca gaactgggta cgccagccgt gcggcggcat
    30721 acccgggccg ggtttatctt ccgaattatc gtgttcaaat gaacgcagga tctgaaagac
    30781 aaagcgatcg ttgttgcgca ggaagcgcca gttaccatgc tgcgcatact gctcgccaag
    30841 cgcatcactt aacagctgta atcgctgttc attgccgtgt ttgatgtaat caataaagcc
    30901 acgctcaaaa ctgatacgca ccgcccagtg catactgatc agcaagacaa tgcaggtggc
    30961 gaaaatcgcc agaaacagtt tgccggtaat accgggtcgc cagaacttca ttgcgcgctc
    31021 ctttttcgcc gcgaaatagc tacattttga tgcgtatcgt tcggcactct ggcaaagatg
    31081 aacgccggaa gggcgatgat aaacgccatg ctaagccagg tgtacataaa gacggtttgt
    31141 gtggtgccgc tgtcgacgct gacatgctgc gaaccaaaaa gccccagcaa cagcccggcg
    31201 atggtgacgc cgatactcat cgacaattgc ataatcatcg acagcaggct gtttccgctg
    31261 ctcgccagat tgtccgggag atctttcagc gtcagggtgt tcatggagga gaaacgggtc
    31321 gagttgacca tcccttgtaa aaacaggacg aacggcaaaa cgtagtacca gcccaacaga
    31381 gcggtagtca taaacaacag ggtgaccagc gacagaccca gcgtggtcgc taccagtacc
    31441 cgacgataac caaagcgatt caccacctgt accacaattc gcttcattcc catgctgcca
    31501 agcaccatcg ggatcatcat cagtccggca tgaaacggcg agaaaccgag gccaatttgc
    31561 aggaaaaccg gtgtcataaa gggcaacatg ccactgccaa tacgtccggc aaagctcccc
    31621 gccaggccca gcgaaaaggt acgagtgcgg aacagtttca gactgaacag ggcgcggttg
    31681 ttatttctgg cgtgcagcag ataaagcacc agtgccacca cgccaactgc gaccaggcca
    31741 gcaatcgcca gcggcgataa acctgtacct ttactgccgt ccagcgccag ggttaatacc
    31801 gccatgccaa ccgccagcaa taaaaagccg gaaagatcaa agcgccgcgt ctgcatggtg
    31861 tagttcggca ttaacatcaa tgtggcgatc gcaccgataa tccccaccgg aatgttgatc
    31921 aaaaagatcc agtgccacga cgcgtattct accagcagac cgccgagcgc cggaccgagc
    31981 agcggaccga cctgaccggg taacgtgaca aaggtcatcg ccgccatata ttgctcgcgc
    32041 ggtacgattt tcatcaccgt caacctgccg accggcacca tcatcgcacc gccaacgccc
    32101 tgtaacgcgc gtgccagcaa cagttcgttc agcgtgccgg aaagcgcgca aaacagtgaa
    32161 ccgagagtaa acagcacgat ggcggtaaag aaaatattgc gcacgccgac tttgtccgcc
    32221 agccagccgc tggcgggcag catcaccgcc acggtcagca cataagagac aatgaccatg
    32281 tgcatatgca acggactttc cccgaggctt tgcgccattg agggaagggc ggtgtttacg
    32341 atggtggtgt ccagcgactg cataaagaag ccgaaagcca caatccataa ttgccagcgg
    32401 gtgctgtcgg gaagatctgt catttactcg gttaccgttt gtttaggttt acgcgaaaaa
    32461 cgcagccgca gacggtcgaa aaagagatac accaccggcg tggtatacag cgtcaggagc
    32521 tggctcatca ccagaccgcc gacaatggtg atcctcaggg gttgccgcag ctccgagccg
    32581 tcaccgcccg acaataccag cggcagcgcg ccaaacagcg ccgccagggt ggtcatcata
    32641 atcgggcgaa aacgcagcag acaggcctgg aaaatagctt cctgcggcgt caggttaccg
    32701 tgccgttggg cttcaagcgc aaaatcgacc atcataatgg cgtttttctt cacgatgccg
    32761 attaatagca tgatccctat cagggcgatt aggctgaacg gggcattgaa cagctccagc
    32821 gccaacagcg ctccaacgcc cgccgagggc agggtggaga gaatcgtcag cggatgtacg
    32881 taactctcgt aaaggatacc cagcacgata tacaccgtgg cgatggcggc gataatcagg
    32941 atcacctgcg agttcatcgt ctcctggaaa acctgcgccg tgccggcaaa actgccgcgc
    33001 accgtcgaag gcacaccaag ctgggtcatt gcgcgatcga tcgccgcact ggcgtccgag
    33061 agcgattttc cggtcggcag gttgaacgag atggtcgagg ccgccgataa tccctgatga
    33121 ttcaccgata gtggggcatt cgccggttgc catttagcga aatacgacag cgggatcgct
    33181 ttgccttcgt tattgataac gaacattttt tccagcgcac tgatgtcctg ggtatagcgc
    33241 ggatccactt ccatcaccac tttatactgg ttcatcggct ggtaaatggt cgagatttgc
    33301 cgctgaccga aggcgttatt taacagactg ttggcggcct gtacgtcgat tcccagccgt
    33361 gccatggtgt cgcggtcgta aaccagattc atctccgcgc cgttatcctg ctgatcggag
    33421 ttcacgtccg ccagttccgg caacgtcgcc agttttttgc ggattttcgg ctcccattcg
    33481 cgcagtgccg ccaggtcgtc ggataacaac gtgtactggt agctggcgtt cgactgacgc
    33541 ccaccaacgc gaatatcttg taccgccatc aggaacagat tcgcccccgg ttctttcgcc
    33601 agcttcacgc gcagacggtc gataatttgc tgcgccgttt cgctgcgttc gtcgcgtggc
    33661 ttgagggtga taaacatcat cccactgtta actcgtgaac cgccggtaaa gccggtgaca
    33721 ttatccactg ccggatcgtc acggataatt ttcatgaaat cctgcaattt gccgcgcatc
    33781 gcctgaaacg aaatactctg atccgcctgt atcccaccca tcagtacgcc ggtgtcctgc
    33841 tccgggaaga aggttttcgg gatcgagata ttcagcgcaa tggtgccaag cagcaccacg
    33901 cccaccagac gggtatgatt gagcacccat ttcagcgact tgccgtagcc ttgttgcagg
    33961 gctaccaaca tgcggccaaa accacgcagt cgcttttgct cacgcggctt gctggctttt
    34021 agcatccagc cgcacatcat tggcgttagc gtcagggaaa ccagcaacga aataccaatg
    34081 gcgacagaaa gcgtcacggc aaactcgcgt aacagtcggc ccggcaatcc gcccatcaac
    34141 agcagcggca ggaacaccgc caccagtgac agactcatcg acagcaccgt aaagccgact
    34201 tcgcgagtac cttgcagcgc ggcctgtagt ggtttcatcc ccgcttccag atgacgggca
    34261 atgttttcca gcaccacaat ggcgtcatcc accacgaaac cagtagcaat ggtgagcgcc
    34321 atcagcgaaa gattattgag actgaatccg cacaggtaca tcgccgcaaa cgtaccaatc
    34381 agcgaaaccg gcaccgcaac ggcgggaata atagtggcgc gacccgagcg caggaataaa
    34441 aagaccacca gaatcaccag cgccaccgag ataatcagcg tttgctcgac ttcttccagc
    34501 gaggcgcgaa tggtggggga gcgatcctgg acaatttgca gatcaatcgc cgccggaatg
    34561 gtctcctgca actctggtaa ttttgcccgg atgctgtcta ccgtctggat aatattggct
    34621 tccggcagtt tgcggatcat cagtaaaata gccggtttgg cgttggtcat cccggcgttg
    34681 cgcacatcct gcactgagtc agtcaccgtc gccacatcgc ccagacgaac cgcgccgccg
    34741 ttgttgtagt gaataatcag cggctgatat tcagcggcgg ttttcagttc atcgttggtc
    34801 tggatctgcc agcggtgagt gccatcttcc agcgccccct gcggtttacg cacgttggca
    34861 ttgctgatgg cagtgcgtac gtcgtccagc gacacgccct gattaaacag cgcctgcggg
    34921 ttcagcccga cgcgcacggc gggcagcgag ctgccaccga catcgacatc accgacaccg
    34981 tcgatttgcg aaatcgtcgg agccagctgc gtcgaggcga aatcgtacag ttcaccctgc
    35041 gaataagtat cggatgtcag cgtgaggatc ataattggcg catccgacgg gttcgctttg
    35101 cgataggtcg ggcggctggg catcccgctg ggcagcaaac tttgtgcagc gttgatcgcc
    35161 gcctgcacat cacgcgctgc gccgttgata tcccggtcaa aatcaaactg caaaataatg
    35221 cgcgtgctgc cgagcgaact gctggaggtc atttcactaa ccccggcaat gcgcccaagt
    35281 gagcgctcca gcggcgtggc aacggaagac gccattgtct ccggcgacgc accgggcagc
    35341 gaggcgctga ccataatcac cggaaaatcg acctgcggca gcggggcgac cggcagcata
    35401 cggaaaccca gtatgccgca cagggtaatg gcgaccgaca gtaaaatcgt cgccaccggg
    35461 cggtaaatga agagggcaaa aaacttcact tacgcctcct cttcatggcg agcaaagcga
    35521 cttttggtcc acaataccag gcggtcgaac agcagataaa tcaccggcgt ggtgaacagc
    35581 gtcagcacct ggctgacaat cagaccgccg accatgccga tacctaaagg acgacgcagt
    35641 tccgcgccga ccccggtact caacatcagc ggcagcgcgc caagcagagc cgccagggtg
    35701 gtcatcagga tcggacgaaa acgcaacaga caagcctggt agattgcatc gcgcggcgac
    35761 atgccttgct cgcgctcagc agccagcgcg aagtcgatca tcatgatggc gttcttcttc
    35821 acaataccga tcagcaaaat aatgccgata atcgcaatca catccagttc gctaccggca
    35881 atcattaatg ccagcagtgc gccgacccct gcggtgggta gcgtcgagag aatggtgatc
    35941 gggtgaataa agctctcgta cagaatgccg agcacgatat acatcgccac caccgccgcg
    36001 acaatcagcc agacagtgct gcccagcgcc gactggaagg cgagggtgct gccctggaat
    36061 tgcgtggtga tatccaccgg cagattcagc gtcttttcgg tgtccataat cgcctgcacc
    36121 gcatcgccca gcgaatagtt atccggcacg ttaaaggaga tggttgttac cgggaactga
    36181 tccagatggt tgatggaaag cggcgcaaaa cgctgctcaa ttttggcaat tgagcttaac
    36241 ggcaccacgc caccgtcgct gctggtcagg cgaatggtat ccagcgccgc gaggcctggg
    36301 gtattttcgg tattgtgctc cagcaccacg cgatactggt tagcctgagt ataaatagtg
    36361 gaaatcagtc gctgaccaaa cgcgttgtac agggcgttat cgacgtccgc catgctgatc
    36421 cccagacggc tggcgctgtc gcgatcaaca ttgacatacg ccaccagccc tttgtcctgc
    36481 cagtcgctgg agacatcaga aagctgtggc agttgctgga gtttttccat caactgtggc
    36541 acccaggtac tgagcgcatc cagtgacgtg gcctgcaagg taaactggta ctgggtgcgg
    36601 ctgacctgag tatcgatggt caggtcctgc gttggttgca ggaagagatc gacgcccggc
    36661 actttatcta ccgccgtttg cagacgggcg atgacttttt gcacccgatc atcacgttca
    36721 tccaacggtt tgaggttgat ttgtaaacgt gcactattca gcgacgggtt agtgccatca
    36781 acgccaacaa atgaggtcag gctttgcact gccggatcct gcaaaatcac gtccgcgacc
    36841 tggcgttgtc gctgggccat attggcaaag gagctggatt gcggtgcctg caaagtgccc
    36901 tgaataatgc cgttgtcctg taccgggaag aaacctttcg gaatgaacac ccacagcagc
    36961 acgctaagca gcagcgtgct gagtgccacg cttaaggtca gccacggatg attcagcact
    37021 ttcgccagtc cacgaccata ggcggcgatt atcctgtcga acattttttc cgaggcacgg
    37081 gagaagcggt tctgtttacg caacgactcc tggctgagca tccgcgcgca catcatcggt
    37141 gtcagggtca gcgacaccac cgctgagatc aaaatcgcta ccgccagggt aatagcaaat
    37201 tcgcggaaca gtcgcccgac gatatcgccc ataaacagca gtgggatcaa caccgcaatc
    37261 agtgagaagg tcagcgagat aatggtaaag ccgatttcac ctgcgccctt gagcgccgcc
    37321 gccaacggtt tttcgccttt ttcgatatag cgggaaatgt tttcgatcac cacgatggcg
    37381 tcatcgacca cgaatccggt ggcgatagtt aacgccatca gtgtcaggtt attgattgaa
    37441 aaatcgagaa acaccataac cgcgaaagtg ccgattaacg acagcggtac agcaacaccg
    37501 ggaatgatgg tcgccggaat attgcgcaaa aacaggtaga taatcatgac taccagcgcg
    37561 atagccatca tcaattcaaa ctgagtatca tcgacggatg cgcggatatt ggtggtgcga
    37621 tcggaaagca ctgtcacctt caccgatttc ggcagactct cagtgagctg tggcagcatc
    37681 tgccgaatgc tgtcggcggt ggagataatg ttagcaccgg gctggcgctg aacattcatc
    37741 acaatggcct gttctttgtt cgcccacgcg ccgagccagc tgttttctgc accttgctct
    37801 acagttgcta catcgcccag acgaattggc gcgccgttct ggtaggcgat gattagctgg
    37861 cgatactctt cggcggattg catctggtcg ttcgcggaaa gcgtgaccgc acgggaaggg
    37921 ccgtcgaggc taccttttgc cgagttaacg ttagcgccgg taatggcggt gcgcacggtt
    37981 tcgctggtca ggccgagggc ggcaatcgcc tgagcgttaa gtttgacgcg aacagccgga
    38041 cgctgaccgc cggaaagcgt caccaggccg acgccggaaa tctgcgagat tttctgcgcg
    38101 acgcgggttt ccaccatatc ttccacttgc gtcatcggca tggcggttga ggtgacggcg
    38161 agcgtcatga tcggcggatc tgccgggttc actttgctgt aaaccggcgg gttaggcaga
    38221 tcgctcggca acaagttggt cgcagcgtta atcgcggcct gcacttcctg ctcggcgaca
    38281 ttgagcggta atgttagctg gaactgcaaa gtgataactg acgcaccgcc ggaactttgc
    38341 gacgacatct gtttcaggcc agacatctgc ccgaactggc gttcgagcgg cgcggtaacg
    38401 gcagaggtca tgacatccgg gctggcacct gggtagagcg tgaccacctg aatggtcgga
    38461 tagtccactt ccggcagcgc cgaaacgggc agggcgcgat aaccgataat cccggcgagt
    38521 aagatcgcca ccatcagcag cgtggtggcg acaggacgca taataaacag gcgcgacggg
    38581 ccgcctgtgc tgctcggggg taacacctgc atcaggagcg tgctcctttt ttcgcgtatt
    38641 cgcggctggt ggctttctct tccggagtgg tggcgctctg ggcttccacc acttccactt
    38701 tctgactgtc ctgaatgccc ggcgtcacca gatgtttgct gaccttgttt tcgctattca
    38761 gcacccagac aaaatggcct tcattgccca tttgcagggc ggctgttggg atcactacgg
    38821 cgttttgttc ggtgtcgact aacatgcgcg cgttaacaaa ctgattggga aacagcgcat
    38881 catcctgatt attaaagcgt gctttcactt taatcgtacc ggttgtggca tcgatttggt
    38941 tatcgaggct taacagcgtg ccttcactta atttcttcga attggtgcga tcccaggctt
    39001 ctaccatcag cggttttccg gctttttgcg cctgcactac agtagcgata tcgctttccg
    39061 gcagggtaaa gactaaatcg ataggatgcg tctgggtgat caccacaatt ccggtggtat
    39121 caccactgga gatttggtta ccaacatcaa cttgcttgag accaacgcga ccatcgactg
    39181 gtgcggtgat gcggctccag tcgagttgca actgcgcgct ggcgacgctt gcttcatcag
    39241 ccttaatggt gccttcggtt tcactgacca gcgcctgttg ggcatccagc tcctgacggg
    39301 agacgagatt ggtttttgcc agttgctgat aacgcgccag atcacggcgg gcgttagcaa
    39361 gcgtggcttt atcttttgcc agttggccct gggtttgtgc taatgcaact ttgaactggc
    39421 tggggtcaat ttctgccagt aaatcgcctg ctttgacctg ctggccttcc tggaaatgta
    39481 acgccatcag ttgaccgtcc acgcggctgc gcaccgtaac agtattagcg gcggtaatgg
    39541 tgccaagccc ggtgaggtaa
//
LOCUS       DADMFL010000025        37643 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.025, whole genome shotgun sequence.
ACCESSION   DADMFL010000025 DADMFL010000000
VERSION     DADMFL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.025"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..174)
                     /locus_tag="JNZ27_001300"
     CDS             complement(<1..174)
                     /locus_tag="JNZ27_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY8663698.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNZ27_001301"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNZ27_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8663699.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNZ27_001302"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNZ27_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY8663700.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNZ27_001303"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNZ27_001303"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY8663701.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNZ27_001304"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNZ27_001304"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY8663702.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNZ27_001305"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNZ27_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY8663703.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JNZ27_001306"
     CDS             complement(5178..5420)
                     /locus_tag="JNZ27_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY8663704.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNZ27_001307"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNZ27_001307"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY8663705.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNZ27_001308"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNZ27_001308"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY8663706.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JNZ27_001309"
     CDS             complement(8337..9311)
                     /locus_tag="JNZ27_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8663707.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JNZ27_001310"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JNZ27_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY8663708.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /locus_tag="JNZ27_001311"
     CDS             11708..12751
                     /locus_tag="JNZ27_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY8663709.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JNZ27_001312"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JNZ27_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY8663710.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JNZ27_001313"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JNZ27_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY8663711.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JNZ27_001314"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JNZ27_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY8663712.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JNZ27_001315"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JNZ27_001315"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY8663713.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /locus_tag="JNZ27_001316"
     CDS             16537..20337
                     /locus_tag="JNZ27_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY8663714.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JNZ27_001317"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JNZ27_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY8663715.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNZ27_001318"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNZ27_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY8663716.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JNZ27_001319"
     CDS             23178..23381
                     /locus_tag="JNZ27_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY8663717.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JNZ27_001320"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JNZ27_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY8663718.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JNZ27_001321"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JNZ27_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY8663719.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFLGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JNZ27_001322"
     CDS             26386..26658
                     /locus_tag="JNZ27_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663720.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JNZ27_001323"
     CDS             complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JNZ27_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY8663721.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JNZ27_001324"
     CDS             complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JNZ27_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY8663722.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28247..29185
                     /gene="mdh"
                     /locus_tag="JNZ27_001325"
     CDS             28247..29185
                     /gene="mdh"
                     /locus_tag="JNZ27_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY8663723.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JNZ27_001326"
     CDS             complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JNZ27_001326"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY8663724.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JNZ27_001327"
     CDS             complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JNZ27_001327"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY8663725.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31925..32323)
                     /locus_tag="JNZ27_001328"
     CDS             complement(31925..32323)
                     /locus_tag="JNZ27_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY8663726.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32517..33644
                     /locus_tag="JNZ27_001329"
     CDS             32517..33644
                     /locus_tag="JNZ27_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY8663727.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33863..34291
                     /gene="rplM"
                     /locus_tag="JNZ27_001330"
     CDS             33863..34291
                     /gene="rplM"
                     /locus_tag="JNZ27_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY8663728.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34307..34699
                     /gene="rpsI"
                     /locus_tag="JNZ27_001331"
     CDS             34307..34699
                     /gene="rpsI"
                     /locus_tag="JNZ27_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY8663729.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35094..35732
                     /gene="sspA"
                     /locus_tag="JNZ27_001332"
     CDS             35094..35732
                     /gene="sspA"
                     /locus_tag="JNZ27_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY8663730.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35738..36235
                     /gene="sspB"
                     /locus_tag="JNZ27_001333"
     CDS             35738..36235
                     /gene="sspB"
                     /locus_tag="JNZ27_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY8663731.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36278..37606)
                     /gene="dcuC"
                     /locus_tag="JNZ27_001334"
     CDS             complement(36278..37606)
                     /gene="dcuC"
                     /locus_tag="JNZ27_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8663732.1"
                     /translation="MGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGVLDPAKSSGYL
                     IVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYI
                     ILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGI
                     LETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQA
                     EQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEF
                     FRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSG
                     LGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATT
                     LARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9114 a         9416 c         9907 g         9206 t
ORIGIN      
        1 tctgtcctga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgcggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaga ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacagt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac gagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagtgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga cgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccaaaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatggaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 tttgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gagccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcaccgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcatat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ttgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggt gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcctagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    26461 ggtctggcta aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    26701 aaacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    26761 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    26821 gtatatccat tggcgaagac gccacaccac ttacggatac ggttccgatt gcttcacgat
    26881 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    26941 gtacgcttaa tgcagctaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27001 ttagctgggc ctttgtttcg tgacccatat cacaaaatca agtacacact aattattaag
    27061 ctaattaata ccacgctaac tatttcagtt gatactaagt gttaaaaatg tgaattttaa
    27121 taacaaaaaa gtataaaaaa cacggttatt tattattaat tatagtattt tcaattagat
    27181 aatgaatttt gcataatgtg ctttttttac cgtatgtatt cattttgtga atgatgtgtc
    27241 gcggtaaaac gcactattcg ttaaatgtat cgcaggagaa agaaggggaa tgagagggat
    27301 aagcaacatt ttccccgccg ccagaagcga cggggcagag attaaagctc ctggtcgaac
    27361 agctctaaaa tcgcttcgta caggtctttg acggtgaaac cgttagcagg ggtggtaaag
    27421 atggtgtcat cgccagcgat ggtgcccaga ataccttctg ctttgcccag tgagtccagc
    27481 aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa tcacgacaac tgcatcgttg
    27541 tagtcgatat ccagcaccag attcttcaat ggactggagg tggttggtac acccagttca
    27601 gctggcaggc agtaaaccat ttccattttg gcattgcgtg tacgtacagc accaaacttg
    27661 gtcaacatcc gcgagacttt agactgatta atattgtcaa agccttgctc ctgcaacgcg
    27721 gcgacgattt cgccctggga gctaaatttc tcttctttaa gtaatgcttt aaatgcttta
    27781 actagttctt cttgcttagc cgagcttcgc ataagtcacc cgatatggtg gttgatacaa
    27841 cattattgtg catacagatg aatttttatg caaacagtca gccctgaaga aggctgaaat
    27901 aatgttatga aagagcggga ttttatcaaa tttcgttatt gagaaacatg cctgcgtcac
    27961 ggcatgcaaa ttctgtttaa aagtaaatta attgttatca aattgatgtt gttttggctg
    28021 aacggtaggg tatattgtca ccacctgttg gaatgttgcg ctaatgcata agcgactgtt
    28081 aattacgtaa gttaggttcc tgattacggc aattaaatgc ataaacgcta aacttgcgtg
    28141 actacacatt cttgagatgt ggtcattgta aacggcaatt ttgtggatta aggtcgcggc
    28201 agcggagcaa catatcttag tttatcaata taataaggag tttaggatga aagtcgcagt
    28261 cctcggcgct gctggcggta ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc
    28321 ttcaggttca gaactctctc tgtatgatat cgctccagtg actcccggtg tggccgtcga
    28381 tctgagccat atccctactg ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc
    28441 ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggc gtagcgcgta aaccgggtat
    28501 ggatcgttcc gacctgttta acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt
    28561 tgcgaaaaac tgcccgaaag cgtgcattgg tattatcact aacccggtta acaccacagt
    28621 tgcaattgct gctgaagtgc tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg
    28681 cgttaccacg ctggatatca ttcgttccaa cacctttgtt gcggaactga aaggcaaaca
    28741 gccaggcgaa gttgaagtgc cggttattgg cggtcactct ggtgttacca ttctgccgct
    28801 gctgtcacag gttcctggcg ttagttttac cgagcaggaa gtggctgatc tgaccaaacg
    28861 tatccagaac gcgggtactg aggtggttga agcgaaagcc ggtggcgggt ctgcaaccct
    28921 gtctatgggc caggcagctg cacgttttgg tctgtctctg gttcgtgcac tgcagggcga
    28981 acaaggcgtt gtcgaatgtg cctacgttga aggcgacggt cagtacgccc gtttcttctc
    29041 tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag
    29101 cgcatttgaa cagaacgcgc tggaaggtat gctggatacg ctcaagaaag atatcgccct
    29161 gggcgaagag ttcgttaata agtaattgat tagcgaataa taaaaaaccg gagcacagac
    29221 tccggttttt gttttgagca ctcgacttaa ttggttgccg gatattcctg aatggtgacc
    29281 tgcagcgtta actgcttatc atcacgcatc actacaaccg ggatcaccga accagggcga
    29341 atttccgcca cctgatccat cgtctccaga gcagagatgg ccggtttgtt atccaccgaa
    29401 ataatcagat cgttgacctg aatacccgca ttcgccgctg ggccgtcagg tgacacttca
    29461 ttaaccacga tcccttgcag ttgatctata ccaccgctct gcgcgtgcag tggtgcgatc
    29521 tcgcgtccgc cgataccaat gtagccgcgg atcacgcgac catcgcggat cagcttatcc
    29581 ataattttgg ttgctaactg gaaaggaatc gcaaagccga taccttccgg cgtttcgcca
    29641 tcgttactct tatcaaacga cagcgtgtta atgcccatca gttctcccag cgagttcacc
    29701 agcgcgccgc cagagttacc gtggttaatg gaagcatcgg tttgtaggaa gttttgccgc
    29761 ccggtcgggt tcagaccgat tcgacccgtg gcactaataa tcccttgggt aatggtctgc
    29821 ccgaggttgt acgggttacc gatcgccagt actacgtcgc caatgtgcgg tacgcgacgt
    29881 gcattaattg gaatggtagg taaaccgcca gtggcattaa ttttaagtac cgccagatcg
    29941 gttagagagt cagatcccac cagcaatgct tcaaatacac gtccatcctg taaggcgacg
    30001 atgatctgat cggcgtcgtt gatgacgtgt ttattggtga tgatataacc gcgttgatcc
    30061 atgattacac cggatcccag ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa
    30121 ccacggttgt aaacgttaac caccgctggc gcggcgcggc gaaccgccag attatagctg
    30181 gcaggcgtct catcggtact gtcaaattgc ggagtggaaa gcgggttaag gctgcgcagc
    30241 gaaggcatgg caaccagcag aatagcgccg acaattaatc caatcgcaac ggaacgtaag
    30301 agcttcacaa acatgatgga ggcgtcatta aaaaagggaa cggcagcagc ataccacgag
    30361 ttaaccggac atcacacgtc agcctgatgc ccggtttacg acattaacgc atcagcagat
    30421 agatgctttc attgccgcgt acaatttgca gggcgatgat ggccggtttt gccgccagca
    30481 ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt gacgccaatg atcacatcgt
    30541 ctttttgcaa gccagcctga gcagctgggc ttcctttgac aacttcatct atcttaatac
    30601 ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc accttccagc gctggcgtga
    30661 tcatttcagc gctggccgac gaagaggtgc tggtatcaag cgtcacttct acttccagtg
    30721 gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac
    30781 gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta ataatatcgc
    30841 ccgctttgac gcccgctttc gctgagccag aacttggcaa cacttcgctg acaaacgcgc
    30901 cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctcg gtgcctttga
    30961 tgcctaacaa accgcgtttg atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg
    31021 ccatattact ggggatggca aatccaatcc cgacgctccc gccgccaggc gcaaggattg
    31081 cagtgttgat gccaattaac tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc
    31141 ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc aacccgctgc
    31201 ggcctaatgc ggaaacaatg ccagaggtgg cggtttgccc aaggccaaat gggttaccga
    31261 ccgctacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta
    31321 atttgctcgg gttttgaatt tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca
    31381 gttttgcatc aaactcgcgc ccatcattga gctgaatact gattttctgt gcctgattaa
    31441 tcacatggtt gttggtcagc acatagcctt tactggcgtt gatgatgaca ccggaaccta
    31501 aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac tttttgaatt
    31561 cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc accactgccg
    31621 gaagcacttt ttccagcatt ggagccagac tggggagagg ggcctgatcg gcaacctggc
    31681 ctggaatcga cgcgacggcc tgaaatgacg ccgagagagt taacccggca cttaacgcta
    31741 atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct gaatgataag
    31801 aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca atgagacacc
    31861 agattaaatg atagtcgggg agggagagaa gaggagggcg caatggctgc gcccgaaaaa
    31921 taaattagtc gcgcttcgcg ccagtacgca gcaggccgga tgcgccttca gaatagtcgc
    31981 gaggcatctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga cgattacgga
    32041 acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttt gccatgtgct
    32101 gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt
    32161 ggctaaccag ctcttcgcga tactcgtcca gttcagcttt attcttttcc agttcgtact
    32221 gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca gcaccaataa
    32281 tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc tcccgttgtc
    32341 ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta tgccactata
    32401 accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag aacggccttt
    32461 ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat aagatcatgc
    32521 aaagcgttac cccaacatcg caatacctga aggcgctcaa tgaaggcagc catcaacccg
    32581 acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaagaa ctcatcaata
    32641 gcaggccacc agcccccagg acgagtgggc taatggcgcg ggtcggtaag ctgtggggta
    32701 aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc ggtgtaggac
    32761 gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag cggaaacagc
    32821 gcctgcactt tcaccgtttt atgctgcggg tgcacgaaga gctaactgcc ttacaggggc
    32881 agaccgatcc gctggaaatt attgccgatc gctttaaagc cgaaactgac gtgctctgtt
    32941 ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggt ctgatgaaag
    33001 ccctgttcac ccgcggtatt accctggtag cgacgtcaaa tattccgccg gacgaacttt
    33061 atcgaaatgg cctgcaacgt gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt
    33121 gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc actcaggcgc
    33181 atctatggct ttcgccactc aacgatgaaa cccgggcgca gatggataaa ctatggttgg
    33241 cgctggcggg ggtgaaacga gaaaattcac cgacgttaga aatcaaccat cggccattgg
    33301 cgacaatggg cgtcgagaac cagacgctgg cggtttcttt tactacgctg tgcgtcgacg
    33361 cccgcagtca gcatgactat attgcgctct cacgcctgtt tcacacagta atgttgtttg
    33421 atgtgccagt catgacgcgg ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg
    33481 atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa
    33541 tttatcaggg tgagcggctg aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga
    33601 tgcaaagcga agagtatctg aagcgcgagc atttggcggg ttaaaacctg ccacaaatca
    33661 caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc acgttacaag
    33721 aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg
    33781 ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa
    33841 ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg
    33901 cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta ctgaactggc
    33961 tcgtcgcctg cgtggtaagc acaaagcgga atatactccg cacgtagata ccggtgatta
    34021 catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt
    34081 gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg aagagatgat
    34141 tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc
    34201 gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc
    34261 ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg ctgaaaatca
    34321 atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa
    34381 cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg aaactgcccg
    34441 catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg acctgtacat
    34501 caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg gtatcacccg
    34561 cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg gcttcgttac
    34621 tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc
    34681 gcagttctcc aaacgttaat tggcttctgc tccggcagaa aacaatttgc gaaaaaaccc
    34741 gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt
    34801 tttgctgccg tctgaaagac aggtaaaaca aaaaccacat cgccaataag ggtctaagtc
    34861 aactatttca gactaaagcg catctctttt tccccatttc cggcatccac tcaccacaaa
    34921 ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg tattgctcat
    34981 tttttgtttg attttcgaac aaagagagtc gttccttatt gggtaacaca acttctgact
    35041 ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt ttcatggctg
    35101 tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac atctatagcc
    35161 atcaggtccg cattgtgctg gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa
    35221 aggacaatcc gcctcaggat ctgattgacc tcaacccgaa tcagagcgtt ccgaccctgg
    35281 tggatcgtga gctgaccctg tgggaatctc gcatcattat ggaatatctg gatgagcgtt
    35341 tcccgcatcc gccactgatg cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca
    35401 tgcatcgcat cgaaaaagac tggtacacgc tgataaacac tatcatcaac ggttcagctt
    35461 ctgaagcaga tgccgcacgt aagcaactgc gcgaagaact gctggcgatt gcgccggtct
    35521 tcggtcagaa gccgtacttc ctgagcgatg agttcagcct ggtcgattgc taccttgctc
    35581 cgctgctgtg gcgtctgccg caactgggca tcgagttcag cggcccgggt gcgaaagagc
    35641 tgaaaggcta tatgacccgc gtctttgagc gtgactcttt ccttgcttct ttaactgaag
    35701 cagaacgtga aatgcgtctg ggccggagtt aatctgtatg gatttgtcac agctaacacc
    35761 acgtcgtccc tatctgctgc gtgcattcta tgagtggttg ctggataacc agctcacgcc
    35821 gcacctggtg gtggatgtga cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga
    35881 cgggcaaatc gtactcaaca ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga
    35941 tgaggtgcgc tttaacgcgc gctttggtgg cattccgcgt caggtttctg tgccgctggc
    36001 tgccgtgctg gctatctacg cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc
    36061 tgcctacgat gaagatacca gcatcatgaa tgatgaagag gcatcggcag acaacgaaac
    36121 cgttatgtcg gttattgatg gcgacaagcc agatcacgat gatgacactc atcctgacga
    36181 tgaacctccg cagccaccac gcggtggtcg accggcatta cgcgttgtga agtaatacaa
    36241 aacaggccca ggcggcctgt tttgtctttt taatgactta ataaaatagc gtgattgtgg
    36301 caatcatatt caccacgaaa ccgactgcca tggggatcgc tgtccgcttc accaccgcaa
    36361 aaggcgaaac gcctgcaatt cctgacgtaa cgaccaccac cgcagtaatc ggcgaaaccg
    36421 cgcgcgctag cgtcgtggca aaatgcatcg gcataatcat tacaaccgct ggtacatgta
    36481 gtccggctgc gatattcgga ataagactgg caaatgacat aaacggcgca ttgccagagc
    36541 ccatcacaat ggcacaaatg gcaatgacta gcgccataat aatcatcacg ccaataccgc
    36601 ccagaccaga atgctccgca cccctgataa ccgcatcaac agtgccaatc gtcgttaagc
    36661 ctttcgcaaa tatttcaccc gcgaccacca gcgttaccac gttgacaaac tgcgtaccca
    36721 tgccgtcaaa aaacgcctgc acatcgtcca ttgtttcgcg caagttatgc ttgcggaaga
    36781 actcaacaaa catcgtcacc gtcaaactta gtaacatcac caccaccaga tgcagttctg
    36841 acttcatcag cccgacgtgg gcgaggaaca gcgagccgag catcaggatt aacggcatca
    36901 caggtaaaat ggcgtaatag agcggcggga cattatcgag agttttttgc tctgcctgtt
    36961 cgtgattgat atttttatct tttttgtcaa aagcgcgttg cacgaaaaag tgggagatcg
    37021 ccaccgagat aatgacgcaa gaggcgaccg gaagctggta gtggaagaag tatgtggcaa
    37081 ttttcattcc cgcgacctgg gctgcaaaaa tggagttcgt ttccagaatc ccccactcaa
    37141 tggacatcgt ggttgcgata accgccaccg cagagaggcg acttactccc agactcacca
    37201 gcgtcggaaa taaggtgacc atcagcaaca tacccagacc ggaggcgctg gtaataaact
    37261 gcgccatgat ttggccgatg acgtaagttg ccgacagaat aatatacggc gagcgaatga
    37321 gttttaacgg gcggcttagc aagctcacca tcgcgcgact ggctcctatg cgctccatgt
    37381 agcgggcata accgcccacc gccataatcg acagccccaa tccggcaatg cggttggaca
    37441 gcatgcgcag gatttcatta taaatgtcga cgatcaagta gccgctgctt ttggcgggat
    37501 cgagtacacc accgaaccct aaccagactc cgcacatcat caggaagaca cctgcggcag
    37561 ccagcaccac ctgaggtttg tagtttttca ggatcaaata gcccatcgta attaatacga
    37621 tgacagatat aattatgccg aac
//
LOCUS       DADMFL010000026        36549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.026, whole genome shotgun sequence.
ACCESSION   DADMFL010000026 DADMFL010000000
VERSION     DADMFL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.026"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(155..1390)
                     /locus_tag="JNZ27_001335"
     CDS             complement(155..1390)
                     /locus_tag="JNZ27_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY8663733.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1537..1613)
                     /locus_tag="JNZ27_001336"
     tRNA            complement(1537..1613)
                     /locus_tag="JNZ27_001336"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1577..1579),aa:Pro,seq:tgg)
     gene            complement(1656..1742)
                     /locus_tag="JNZ27_001337"
     tRNA            complement(1656..1742)
                     /locus_tag="JNZ27_001337"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1706..1708),aa:Leu,seq:cag)
     gene            complement(1763..1838)
                     /locus_tag="JNZ27_001338"
     tRNA            complement(1763..1838)
                     /locus_tag="JNZ27_001338"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1803..1805),aa:His,seq:gtg)
     gene            complement(1897..1973)
                     /locus_tag="JNZ27_001339"
     tRNA            complement(1897..1973)
                     /locus_tag="JNZ27_001339"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Arg,seq:ccg)
     gene            complement(2076..3461)
                     /locus_tag="JNZ27_001340"
     CDS             complement(2076..3461)
                     /locus_tag="JNZ27_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8663734.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="JNZ27_001341"
     CDS             complement(3652..4392)
                     /gene="rffM"
                     /locus_tag="JNZ27_001341"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY8663735.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="JNZ27_001342"
     CDS             complement(4395..5747)
                     /gene="wzyE"
                     /locus_tag="JNZ27_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY8663736.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="JNZ27_001343"
                     /gene_synonym="wecF"
     CDS             complement(5744..6823)
                     /gene="rffT"
                     /locus_tag="JNZ27_001343"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY8663737.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="JNZ27_001344"
     CDS             complement(6820..8070)
                     /gene="wzxE"
                     /locus_tag="JNZ27_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY8663738.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNSDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8072..9202)
                     /locus_tag="JNZ27_001345"
     CDS             complement(8072..9202)
                     /locus_tag="JNZ27_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY8663739.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="JNZ27_001346"
                     /gene_synonym="wecD"
     CDS             complement(9207..9881)
                     /gene="rffC"
                     /locus_tag="JNZ27_001346"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY8663740.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="JNZ27_001347"
     CDS             complement(9859..10740)
                     /gene="rfbA"
                     /locus_tag="JNZ27_001347"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8663741.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="JNZ27_001348"
     CDS             complement(10759..11826)
                     /gene="rffG"
                     /locus_tag="JNZ27_001348"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8663742.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="JNZ27_001349"
     CDS             complement(11826..13088)
                     /gene="wecC"
                     /locus_tag="JNZ27_001349"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY8663743.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="JNZ27_001350"
     CDS             complement(13085..14215)
                     /gene="wecB"
                     /locus_tag="JNZ27_001350"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY8663744.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="JNZ27_001351"
     CDS             complement(14271..15317)
                     /gene="wzzE"
                     /locus_tag="JNZ27_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY8663745.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASVDQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="JNZ27_001352"
     CDS             complement(15329..16432)
                     /gene="wecA"
                     /locus_tag="JNZ27_001352"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY8663746.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16672..17931)
                     /gene="rho"
                     /locus_tag="JNZ27_001353"
     CDS             complement(16672..17931)
                     /gene="rho"
                     /locus_tag="JNZ27_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY8663747.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="JNZ27_001354"
     CDS             complement(18016..18117)
                     /gene="rhoL"
                     /locus_tag="JNZ27_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY8663748.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="JNZ27_001355"
     CDS             complement(18258..18587)
                     /gene="trxA"
                     /locus_tag="JNZ27_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY8663749.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18718..19983
                     /gene="rhlB"
                     /locus_tag="JNZ27_001356"
     CDS             18718..19983
                     /gene="rhlB"
                     /locus_tag="JNZ27_001356"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY8663750.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20117..21601
                     /gene="gppA"
                     /locus_tag="JNZ27_001357"
     CDS             20117..21601
                     /gene="gppA"
                     /locus_tag="JNZ27_001357"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY8663751.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21648..23669)
                     /gene="rep"
                     /locus_tag="JNZ27_001358"
     CDS             complement(21648..23669)
                     /gene="rep"
                     /locus_tag="JNZ27_001358"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY8663752.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFLSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23756..24037
                     /gene="ppiC"
                     /locus_tag="JNZ27_001359"
     CDS             23756..24037
                     /gene="ppiC"
                     /locus_tag="JNZ27_001359"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY8663753.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="JNZ27_001360"
     CDS             complement(24083..25558)
                     /gene="ilvC"
                     /locus_tag="JNZ27_001360"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY8663754.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25708..26598
                     /gene="ilvY"
                     /locus_tag="JNZ27_001361"
     CDS             25708..26598
                     /gene="ilvY"
                     /locus_tag="JNZ27_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY8663755.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="JNZ27_001362"
     CDS             complement(26595..28139)
                     /gene="ilvA"
                     /locus_tag="JNZ27_001362"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY8663756.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="JNZ27_001363"
     CDS             complement(28142..29992)
                     /gene="ilvD"
                     /locus_tag="JNZ27_001363"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY8663757.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDVMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="JNZ27_001364"
     CDS             complement(30057..30986)
                     /gene="ilvE"
                     /locus_tag="JNZ27_001364"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY8663758.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="JNZ27_001365"
     CDS             complement(31006..31269)
                     /gene="ilvM"
                     /locus_tag="JNZ27_001365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY8663759.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31266..32942)
                     /locus_tag="JNZ27_001366"
     CDS             complement(31266..32942)
                     /locus_tag="JNZ27_001366"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY8663760.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGIPKDIQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVG
                     MAQAVPALREFLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLL
                     IAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQP
                     LNINDWQQHCAQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWA
                     AQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGT
                     VKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHI
                     TRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32945..32995)
                     /locus_tag="JNZ27_001367"
     CDS             complement(32945..32995)
                     /locus_tag="JNZ27_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663761.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33081..33179)
                     /gene="ilvL"
                     /locus_tag="JNZ27_001368"
     CDS             complement(33081..33179)
                     /gene="ilvL"
                     /locus_tag="JNZ27_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY8663762.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33531..35051
                     /locus_tag="JNZ27_001369"
     CDS             33531..35051
                     /locus_tag="JNZ27_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY8663763.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35076..35414)
                     /gene="maoP"
                     /locus_tag="JNZ27_001370"
     CDS             complement(35076..35414)
                     /gene="maoP"
                     /locus_tag="JNZ27_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY8663764.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35533..36372
                     /gene="hdfR"
                     /locus_tag="JNZ27_001371"
     CDS             35533..36372
                     /gene="hdfR"
                     /locus_tag="JNZ27_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY8663765.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36468..36543)
                     /locus_tag="JNZ27_001372"
     tRNA            complement(36468..36543)
                     /locus_tag="JNZ27_001372"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36508..36510),aa:Trp,seq:cca)
BASE COUNT         8929 a        10001 c         9203 g         8416 t
ORIGIN      
        1 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca cagaacgctg
       61 caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc gcatccggca
      121 atcaaccgca ggcggcttcc aaatggccgc ttttttactt attcactacc atcaaatgtg
      181 cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt gctttaagat
      241 atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc agccccggtt
      301 tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccaacat gcttttaaca
      361 cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca aacacctgct
      421 gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc agccgatatt
      481 gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca atcaccgagc
      541 gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa ttggcaaacg
      601 cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag tggtcaaaaa
      661 tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc agttccccct
      721 gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca tcagccaggc
      781 gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa aaatcataaa
      841 cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta tagtcgacat
      901 gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt ctgccgcctt
      961 tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac ccaacaagaa
     1021 aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg ttagtctgga
     1081 aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg gcacggcggt
     1141 aaaactccag cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc acttcgtttt
     1201 gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca tcgtccatat
     1261 gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag tagtcacagt
     1321 tcagattaca atcggaaccg ctcggtttcg ccatcacatg aaaagcacgc gttggaacct
     1381 gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg gcgaaatcat
     1441 accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac gaaatgcaca
     1501 gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga ggattcgaac
     1561 ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgaaatact
     1621 gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg gacttgaacc
     1681 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     1741 gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac gacaactgga
     1801 atcacaatcc agggctctac caactgagct atagccacca ctacaaatct tgttacgcgg
     1861 tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca ggattcgaac
     1921 ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg cgcttagcgc
     1981 ctttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt tttaaataaa
     2041 ttgctcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt atgcgcttta
     2101 ccgtggcgat taagcccaaa aactttataa atcgccgtca ccgccagcat aaagatgatg
     2161 ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa aacgcaaatc
     2221 aggaatgcca ttgttatgta gttggcccac gggaacagga ttgagcggaa cggatggctg
     2281 gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat cacaaaccat
     2341 ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg ctgcggattg
     2401 ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac tgccacaccc
     2461 gcaaccggta cgccgtgacg ggaaactttt gccattgccg ccggtaactg acggtttttc
     2521 gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc agagagcgca
     2581 gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat tttggcaaaa
     2641 gtcagtacga acgggctgcc gttgctgcca atttcattcc acgggaagat ggtgacgata
     2701 acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcactttgcc tacggcactg
     2761 cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag ctccacgccc
     2821 tggtaggacg ccaccacaat acacagagcg gtcaggaacc ctttccagcc acccgcaaag
     2881 aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt gccaaagcca
     2941 aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac tttgatcatc
     3001 gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc caacgccacc
     3061 agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccggggacca aaactggaca
     3121 taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa ccagtaagac
     3181 caggcggtga gatagccaaa aaacgggctc atataacgat gcgcataaac ggcgaacgaa
     3241 ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa aaaaacgaac
     3301 agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag ggtactggcg
     3361 gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc gatatgtcga
     3421 gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg tgttgtgttt
     3481 gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc gaatgttttc
     3541 gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt gtaataaaaa
     3601 agcagacagg cgacgaagtg accactccgt cgctttacaa agagaggaaa atcagaggtt
     3661 gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa tgcggctcgg
     3721 ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt tcggtgcgcg
     3781 ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca gcgcatctgg
     3841 atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca tcgcaacggt
     3901 gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct gctcgggttt
     3961 aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca gtttagcttc
     4021 agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc cttctttgcc
     4081 tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa cctgcgcctg
     4141 cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt taaattcggc
     4201 agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt tttcggcatt
     4261 aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat cgagggcatg
     4321 ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg gtgccgtggt
     4381 gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt acgtttatga
     4441 atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag acatgcgccg
     4501 aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc acgtgccagc
     4561 acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc cttatagcga
     4621 ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat cagtccaacc
     4681 acaatcgccc cgagtgggat gaacaacgcg ccgcccatca ccaccagtga gcctataagc
     4741 gtaggcgaga tcgccagtcc ggagtggtta ttcagtactt cccaggtaaa gtaattggct
     4801 gagttcagca ccatactcgg gcgccccggc cacagccagg aagggataaa gacatagaaa
     4861 tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg caacagcaac
     4921 gccagattct cccacggtga aaaggtgtcg cgagtgagat agagaaacgt atagaacgct
     4981 tcatcgccgc tcacattcat tccatagcgt tttaacgcca gccagaacat gccaacaatc
     5041 cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat aatgccaata
     5101 aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac aatcatataa
     5161 gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt gctgtcctgg
     5221 cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg ttttaacgcc
     5281 acgccggaga cttcactgga aaagatctga ctgtaggagt taagccggaa cagcaaaaag
     5341 ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag gatcacccac
     5401 gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac atcagcaacg
     5461 cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca gcccgcagaa
     5521 agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg aaataccagc
     5581 acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga aaagaagaca
     5641 ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat aaacagcgtg
     5701 cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg acctccccgg
     5761 cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag aaggcaatgg
     5821 tgtttttatc cgccgacgcc aactggcgct gcgcttcacg cacaatatcc tcgttgagat
     5881 cgtcggtagt aaacagcact ggcaaatgct gttccgtcat atcctgccag aacggatttt
     5941 ccctgttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta ccaatgccct
     6001 gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc agataggcgt
     6061 caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac tccagccccg
     6121 cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc ggcaccacca
     6181 cttttaccgt atcgccaaat tgctgatgaa cggcacgtaa agcggcgaca tgctcattgc
     6241 tgcggtcgcc ggagttcccc accagaatgg tcatttttcc ttcacgttgc cgatcgttcg
     6301 ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttct ccccgcacct
     6361 ttgggtgcgt tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca cagccgactc
     6421 gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca ctggaaagct
     6481 catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg ccaccactca
     6541 gcagagccag ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc tgctgacggt
     6601 tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg aaaaattgca
     6661 cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc ataaactcgc
     6721 acgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg gttcggttat
     6781 ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc tacgccagag
     6841 taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat atgcctgcgc
     6901 cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca ataaagtgaa
     6961 ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca ccagataacc
     7021 aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa agagatcgcg
     7081 cataacggta aatttattcg acaacagcaa ccagatagca aaatcacgca gcaaccagac
     7141 ggtaaaactc gctgccgcca ctgccggtaa gacgaatttc agcgatttaa ccacttcccg
     7201 ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata cgctgaacga
     7261 tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc agatccccac
     7321 ttcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag caacaggcaa
     7381 ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg ccagaccgtt
     7441 atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttaatca acatgatggc
     7501 ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc cttcataacc
     7561 gcccaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga caatcagcga
     7621 taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca ggttgcccca
     7681 ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt cggaattacc
     7741 aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca tcagcgtaga
     7801 gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt gcggattatc
     7861 atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagccccgg caagcacgcc
     7921 gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg gaccaaatga
     7981 caccgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg tactggccgc
     8041 cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac aaagtcgcaa
     8101 ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc agcaggcgct
     8161 cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagtgt tcccccgcag
     8221 ggcaaccgtg cagcggaatg taatgaaaca ccgccatgat ttccgcttct ttcagaaagt
     8281 taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata tgcgcgttct
     8341 gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc agaggcgcta
     8401 acgtatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga tccgctgctt
     8461 ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg gagccaatat
     8521 cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta gtgccctttt
     8581 cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc gcgccgcctt
     8641 caccacccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata tgaccaatgg
     8701 ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct tccaccacaa
     8761 acaaattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc acacccgcgt
     8821 aatggaccgg cacgataacg cgcgttttgt cggtgatcgc tgcttcaatc aacgtttcgt
     8881 cgatgttcat ggtgtccggg cgaacatcca caaagacgat tttcgcgcca cgcagcacaa
     8941 aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg ccaggctgga
     9001 tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc agtaacactt
     9061 tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa ccgccatcgc
     9121 cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt cccaccaccg
     9181 gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt gctttctaca
     9241 ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg ggtcgccacc
     9301 cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat cagctcagca
     9361 cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag ttcccgtaaa
     9421 gagacatagc cgcgaatatc gccggacgcc gcacgtagaa tcagacattg atgatcaaag
     9481 gtgccgcgca cggcattttc aatccactgt gcataaaagc gaccgctggc gtcaggcgca
     9541 taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tagctaactg acgtaatgcg
     9601 ggaatatcgg tctcttgtgc cacgacagca ccgctatcac tgacattgtt cacgggtagc
     9661 gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc atccagttcc
     9721 cccgtatttg atgccgcaat tttggcctgc acccgtgacc atggcgctaa ggcgtctgac
     9781 gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc aaagaaggcg
     9841 ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact ccagcagata
     9901 ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct cgtcatcgag
     9961 ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct ggcgtttttc
    10021 tactgtctgc acaaaagtgc tggcctcaat caggctgtcg tgagtgtcgg tatccagcca
    10081 ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt acatctggtt
    10141 gatggaggta atctccagtt caccgcgctc cgacggcttc acctgctttg cgtactccac
    10201 gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg gctgttttgg
    10261 cttttcttca agcgagatag cgcggaaatt gtcgtcaaat tccaccacgc caaagcgttc
    10321 cgggtccatc acctgatagt caaaaaccgt cgccccttcc gtgcgcgccg caacatgacg
    10381 cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca gacaagaagg
    10441 ttcaccatta aggaaggttt caccgatgat aaaggcctgc gccagaccgt ccgggctggg
    10501 ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca ggcgctggaa
    10561 ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg ccagcatcag
    10621 caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt gcttcgatac
    10681 gccgcgcgta atcggatgca atcgggtacc ggaaccgccc gccaggataa tacccttcat
    10741 gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcaccctgat agctgccgtc
    10801 ctgcacctgc ttccaccagc tttcattagc cagataccac tgaaccgttt tacgcattcc
    10861 actttcaaag gtttcctgcg gcagccagcc aagttcacgg gcaattttcg aagcatcaat
    10921 ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt cacgatagtg
    10981 cgccacgccg tgcggcttgt tcgaagccag ttcttccagc agctcgcaaa tggtttccac
    11041 aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac cgactttccc
    11101 ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc agtcacggat
    11161 ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga ggatcatcag
    11221 cgggatcagt ttttccggaa agtggtaatg accgtagtta ttcgagcagt tggtgatcag
    11281 cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc tggcttttga
    11341 cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat catccgtcga
    11401 gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg ctgatttttt
    11461 atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat aagtcccgac
    11521 aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat ggctttcggc
    11581 tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca gttctgcccg
    11641 atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg gtgccagcga
    11701 catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc tcgtttcgtt
    11761 gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta tcagaatttt
    11821 tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac attgtcgcca
    11881 ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc cgttgccagc
    11941 gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa ctggtggatg
    12001 ttaggctcaa caaccagcgt ttcgccgctg tgccactggg cgatcagttc agcgatttcc
    12061 atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc aaagcaggcg
    12121 attttcagtt cactggcgcg tttatcggta gcagcaaggc agtcggcaac cgccgctttc
    12181 acctgatcga taacccagaa cggtttgtga tcgttaactt cgcgcgcggt acggataagc
    12241 cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat gcagtgaccg
    12301 cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc caggcgaatc
    12361 agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt agcaaaagcg
    12421 atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt ccgcgagtta
    12481 gtgacgacac actcaccttc gaggaaaatt ttgtacaatt cgctggcgcg ggccgaacaa
    12541 accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat aacctggccc
    12601 ggtaatacac gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc cacctgctgt
    12661 gggaaagtca gatccgggcg catctctgct aaccattcag ccatcttctc ggttgacccc
    12721 accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg cgcaatggag
    12781 cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa cggcgtaggt
    12841 acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa aaaaccgcct
    12901 tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat ttcgccacga
    12961 ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt ttgccgtgag
    13021 gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga aatggtcgca
    13081 aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag catgcctgac
    13141 catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg tcttttaaaa
    13201 gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc accgtacccg
    13261 ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt ttccccagcg
    13321 aaggcgcttc ttcctgaatg ccgcctgagt cagtcaaaat cagccaggcg tggttcatca
    13381 gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc acatgcccca
    13441 gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag acaatctgga
    13501 tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca gatttcttca aagccacgac
    13561 cgaaactctc acgcctatga ccggtcacca gaatcatctt tttatcgggg gcgataaacg
    13621 ggtaatttgc cgccagttct gaacgcagcg tgtcgctgct catcacctgg tcacgcaccc
    13681 ataacagtgc atcaatgact gtattaccgg taatgaagat tcggctatcc gcaacgtttt
    13741 cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc gccagatgcc
    13801 cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc gtgcgcagac
    13861 cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg gttgccagcg
    13921 tcgtcgtcgt atcgccgtga accagcacaa cgtctggttt gaactcggca agaataggtt
    13981 ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc tgcattatgt
    14041 tgagatcgta gtcaggtaca atggaaaaga gtttcagtac ctgatcaagc atctcccgat
    14101 gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac gcatgcacca
    14161 acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact ttcacatcga
    14221 ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg ctatttcgag
    14281 caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc ccacataatc
    14341 atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt acgcaaatag
    14401 cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt taacatggct
    14461 cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt ttccagtcga
    14521 gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc ctcggcaggc
    14581 acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc ctgctcaatg
    14641 ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt cacctgagct
    14701 ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag atggctggct
    14761 gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc aggcgcggtt
    14821 tcggcaataa gcttcacgct gtcattgacc gcgcgagtaa agtctccggg gataaactgg
    14881 atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc caccatccgc
    14941 tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga ggccagttgc
    15001 ataacaaact ctttataggc ttcgtccatg accgatggtt gatcgacaga agccatgttt
    15061 gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc cagcatattc
    15121 accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa aaaagtatac
    15181 gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt cccagcccac
    15241 aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc cggtttccca
    15301 ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg ccaccacgat
    15361 ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag gcacgcttaa
    15421 tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc cactccggga
    15481 caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg gcaagggtaa
    15541 tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg tgaatatgct
    15601 gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata atcgccacca
    15661 tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg ggatgggttt
    15721 tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc gtactgcccg
    15781 catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc atgatgtatg
    15841 gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc ccgtcgaacc
    15901 acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc aagccatcaa
    15961 tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc gtcaggaagt
    16021 aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg ctactgagat
    16081 aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg gcacggattt
    16141 ttacgctgat atcaaaacga tcatccagcg cgccaatgaa aacaagcaca ccggcacaag
    16201 cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg aagcaaatcc
    16261 ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt ttgcggaagt
    16321 ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac agaaacagtg
    16381 tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc acaatgcatg
    16441 ctctcagaga aaattattag cagaagtata accacgaaga cctttattca gaagggaaag
    16501 tccgttccga acccaccagc cctgtgcaaa aattaaccat ttgatatgca aattgcataa
    16561 catgccagca aattccagga attttcctaa catcgccagc gcgacattaa gattacagat
    16621 ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa tttatgagcg
    16681 tttcatcatt tcgaagaaat cgtcattggt cttggtcatg gccagtttat taatgaggaa
    16741 ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc acattttctg
    16801 cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt tgtagtcgat
    16861 agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca tgttgcctgt
    16921 acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga taagcgccgt
    16981 cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccaa agaagcgttt
    17041 cggacgatga agggcattgg cgtccacacc accggtcaac actttacctg acgccggaac
    17101 aacagtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga taacgtcttt
    17161 cttgtgctca accaggcgtt tggccttctc gatcaccatt tccgcaacct gaacgtggcg
    17221 agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac gctgcatctc
    17281 ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat ccgggtggtt
    17341 gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt ttaccggctt tcggcggtgc
    17401 cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta cgcgagcagt
    17461 taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg cgtgcagcgg
    17521 ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga agttaacttc
    17581 gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa tcttaccaga
    17641 gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa cgtagatgtc
    17701 atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc catcctgcaa
    17761 tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct gcttcaggat
    17821 ggcaaaaata atgtcctgct tacgcatgcg agccaggttt tccagcccca tattttcgcc
    17881 gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca tagtggtgtg
    17941 agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg caggatcatc
    18001 catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag gagagtacgc
    18061 agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac ttcgcattta
    18121 agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta tgtttaatac
    18181 gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc gacggggcac
    18241 ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa ctgaccttta
    18301 gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg
    18361 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca
    18421 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg
    18481 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca
    18541 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata taactccaca
    18601 ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact aaaggttgac
    18661 tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta ccacactatg
    18721 agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc gaaggttgta
    18781 gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact ggcccttccg
    18841 ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg gaaaacgatg
    18901 gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc cgatcgcaag
    18961 gtgaatcaac cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt gcagatccac
    19021 gccgacgcgg aaccgctggc ggaagctacc ggcctgaagc tgggcctggc ttacggtggt
    19081 gatggctatg acaaacagct gaaagtgctg gaaagcggcg ttgacattct gattggcacc
    19141 acgggtcgtt taattgacta cgccaagcag aaccacatta acctcggtgc cattcaggta
    19201 gtggtactgg acgaagccga tcgcatgtac gatctaggct ttattaaaga tatccgctgg
    19261 ctgttccgcc gtatgccgcc tgcaaatcag cgcctcaaca tgctgttctc cgccacgctt
    19321 tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata tattgaagtg
    19381 gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc ttctaacgaa
    19441 gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag agcgattatt
    19501 ttcgccaata ccaaacaccg ttgtgaagag atctggggcc acctggcggc agatggtcat
    19561 cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat tcttgatgaa
    19621 tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg tggtttgcat
    19681 attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga agattacgtt
    19741 caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag cctggcgtgt
    19801 gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc aattccggta
    19861 agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg cctcacgcgc
    19921 ccgcgcacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg tcgttcaggt
    19981 taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag agaatgaatc
    20041 ccgatgagct tactgtagta agtgattcgg gtgaaagaat gcagccaaca cagagacaga
    20101 ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg atctcggttc
    20161 gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga cgctgacgcg
    20221 aattaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgacc tctctaatga
    20281 agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc aagatatccc
    20341 tccctcgcaa attcgcgttg tcgctacggc gacattacga cttgccgtca atgcgggtga
    20401 ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta caggtgatca gcggtgaaga
    20461 ggaagcgcgt ctgatttatc agggcgttgc tcacaccact ggtggtgccg atcagcgcct
    20521 ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg cacaaaccac
    20581 ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt ttgccgatcg
    20641 taatctgggg caagaaaatt ttgatgctgc agaaaaagcg gcacgcgaag tgttacgtcc
    20701 ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt ccggcaccgt
    20761 gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta ccctggaaaa
    20821 gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac tggagattga
    20881 cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc tgatcgccat
    20941 ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc gtgaaggcct
    21001 ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta cactgcgtaa
    21061 tattcaacgc cgctttatga tcgatattga tcaggcacag cgcgtagcca aagttgcggc
    21121 taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa gcaataagcc gcgatttgct
    21181 catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac aagctccgca
    21241 acacgctgct tatctggtgc gtaacctgga tcttcccggt tttacccccg cacagaagaa
    21301 actgctggcg acgctactgc tcaaccagac taacccggtc gatctctcat cgctgcatca
    21361 gcaaaatgcc gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc gcctggctat
    21421 catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac aggctaacca
    21481 tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc tgggtaaaga
    21541 gattattgct caggaaaacc agtggcagag ctatgtccac tggccgctgg aagtgcatta
    21601 atgagtaagt gccggatgcg atgctgacgc atcttctccg gccttgatta tttccctcgt
    21661 tttgccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg catccgttct
    21721 tcggcactga ccactttgcg ctcctgttcc caaatcagat catcctgcgg cagctccagc
    21781 aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact gacggcgttc tttacacagc
    21841 gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg ccgctcctca
    21901 tcgatattat cttcatcgat actgctctgg tgcggcaaaa acccttcttc cataccgacc
    21961 atgtagacat aaggaaactc cagccctttt gacgcatgga gagtcatcag ttgcacctga
    22021 tccagctctt cttcactctc accacgctcc atcatgtcgc gtaaagtaaa gcgcgtcacc
    22081 acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc cgtcatccag
    22141 ctaaacagtt ggttgacgtt cttcatgcgc atttcggcag ctttcgggct gggcgatgtt
    22201 tcgtacagcc aggattcata atccatgccg tggatcagat cgcgcaccgc ggcaatcggc
    22261 tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt caatgcttca
    22321 taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt aaacatgctt
    22381 ttattgcgcg tcatcgccca ctcacccagc tttttcagcg tcgccgggcc aatctctcgc
    22441 ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt cagcacgcgc
    22501 agataagcca gcaagtcctt gatttcagga cgagagagaa acgacgtacc accagaaatt
    22561 ttgtacggga tgcggttttg catcaggaat ttttcaaaca cccgcgactg atggttaccg
    22621 cgataaagaa tagcgtaatc tttgtactgc gttttattga cgaagtgatg cgcaatcagt
    22681 tcaccagtga cgcgctcggc ttcatgttct tcgttattcg cgcttaaaac ttttagctct
    22741 gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt attggcgatc
    22801 aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc aagcttaatc
    22861 accttcagcg ccggaaaatc ctggctcagc agcaccaggt tttgcggacg tgcgccgcgc
    22921 caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg gctgcccacc
    22981 agcagtttca ccagttcata ctggctggtg ttggtgtcct gatattcgtc caccagcagg
    23041 tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag aagtagcgtc
    23101 ggcaataaaa tcagatcatc aaagtcgagc acattacagg ctttcaggtg tgcatcatac
    23161 agcccataac aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc tgccgcctgg
    23221 gacggtgttt tgagatcgtt cttccagtta gagatggtcg aaatcaactg ttgcaggaga
    23281 actttgtcat cttcaatcag cccttcggtc aactctttga gcaacgccag ctgatcggta
    23341 tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg catactcacg cttgatgata
    23401 tccagcccca gcgtgtggaa ggttgagatc atcagcccac gcgcctcttt gcgacccagc
    23461 gtctgtccga cacgctcttt catctcgcgc gccgccttat tggtaaaggt caccgccgcg
    23521 atatgtcgcg cctgataacc gcacccgcgg atcagatggg cgattttatt ggtgataaca
    23581 cgagttttac cggaacccgc acccgccagc accaggcagg ggccggtaac gaattcgaca
    23641 gcttgttgtt ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag tcgggagggg
    23701 aaaataacag cgtggtagta tagccagcct catccttacc actcaaggca cgatcatggc
    23761 aaaaacagca gcagcactgc atatccttgt aaaagaagag aaactggctc tggatcttct
    23821 cgtgcagatt aagaacggtg ccgatttcgg caagctggcg aagaaacact ccatttgccc
    23881 atcaggcaaa cgcggcggtg atttaggtga attccgccag ggtcagatgg ttccggcgtt
    23941 cgataaagtt gtgttctctt gtccggtact ggagccaacc ggcccgctgc acacccagtt
    24001 cggttaccac atcattaagg tgctgtaccg taactaatag caaggccttc tccgggagaa
    24061 ggccttgaac tctgaatcgc tattaacccg caacagcaat acgtttcata tccgtcatat
    24121 agccgcgcag tttcttacct acttgctcaa tcgcatggct gcgaatcgct tcatttacat
    24181 cacgcagctg cgcgttatct accgcacctt ccggaatagc tttacccaaa tcgcccggtt
    24241 gcagttctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac agatagttac
    24301 cgtactcggc agtatcagag ataaccacgt tcatttcata cagacgctta cgggcgatgg
    24361 tgttggcaat caacggcagt tcgtgcagtg attcatagta agcagattct tcgatgatgc
    24421 cggaatcaac catggtttcg aacgccagtt caacgcccgc tttcaccatc gcgatcatca
    24481 gtacgccttt atcgaagtac tcctgctcac cgattttgcc ttcatactgc ggcgcggttt
    24541 cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc
    24601 agtccaccat cataccggag gagaattctc cggagatgat gtcgtccata tgtttctgga
    24661 acagcggtgc catgatctct ttcagctgtt cagacaacgc gtaagcacgc agtttcgccg
    24721 ggttagagag acggtccatc atcagtgtga taccgccctg cttcagcgct tcggtgatgg
    24781 tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc tcttccacca
    24841 gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg gtttgctcac
    24901 ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc gcacggtgac
    24961 caccggttgc tgccgcccat gctttagcga tcgccatgcc ttcgcctttc ggatcgtttt
    25021 ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgcactt
    25081 cggtgccagg gcatttcggc gcaaccatga ctacggtgat gtctttacgg atctgctcgc
    25141 ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg
    25201 gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat
    25261 ccgcctgtgg gatcagttct tcgtaagtac ccactttaaa accattttcg gtcgctttac
    25321 gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatcgagac
    25381 cagaatcacg catgttcaga ccctggttca gaccttgtgc gccacagccg acgatgacta
    25441 cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc ataaagcgac
    25501 atttgcccag ctgtgccagc tgctgacgca gattcagtgt attgaagtag ttagccatgg
    25561 tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac
    25621 tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct
    25681 gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat
    25741 ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg
    25801 ctctcacggc agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat
    25861 aaccgcacgg tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg
    25921 ctgttgcagt atcagcagct gcgccacacc atcgaccagc aagggccgtt gctctatggc
    25981 gaattacata tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc gattctggat
    26041 cgcttccgcg cggaacaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat
    26101 gcaatggaaa aagtcgtcac aggtgaagct gatctggcga ttgccgggaa gccagagact
    26161 ctgcccggcg cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc
    26221 gcattgccct gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta
    26281 ccgtttatca tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc
    26341 aataaaatca gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg
    26401 atggtggcgc tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc
    26461 gaaccggtgc gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag
    26521 ctgggcgtgt gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag
    26581 attttgccga accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg
    26641 tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt
    26701 tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa
    26761 atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg
    26821 aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag
    26881 cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg
    26941 ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca
    27001 aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg
    27061 ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac
    27121 aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag
    27181 ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg
    27241 atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca
    27301 tcttcgaata aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg
    27361 tcgagatact cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct
    27421 tcggcgaaca gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc
    27481 aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg
    27541 atcagcaccg ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg
    27601 agatgggagt cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc
    27661 ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta
    27721 gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc
    27781 gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac
    27841 cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg
    27901 tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag
    27961 ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac
    28021 agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc
    28081 actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt
    28141 attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc
    28201 tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt
    28261 tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta
    28321 actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa
    28381 tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc
    28441 cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga
    28501 agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga
    28561 tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct
    28621 ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcag
    28681 ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt
    28741 tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat
    28801 cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga
    28861 acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg
    28921 gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt
    28981 caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg
    29041 tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca
    29101 tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag
    29161 agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat
    29221 tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc
    29281 gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt
    29341 tgcccggctg cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca
    29401 tcccggagca ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt
    29461 cagaaacttt cgggtctgcg ccctggatca tcacatcaac cagatcgagt ttgattatct
    29521 gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa
    29581 tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc
    29641 agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt
    29701 cgcgagatgg aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg
    29761 caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt
    29821 taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg
    29881 cgataatcgg cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag
    29941 cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt
    30001 tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat
    30061 tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag
    30121 aaggcttgct gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg
    30181 tctacgctgc gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc
    30241 aagtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc
    30301 agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg
    30361 aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg
    30421 ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc
    30481 agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg
    30541 cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac
    30601 gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact
    30661 cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt
    30721 ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgtga aaccgggaag
    30781 cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc
    30841 ggtcctttgt gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc
    30901 gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa
    30961 atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg
    31021 ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa
    31081 ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt
    31141 ttgtgcatcg ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac
    31201 cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg
    31261 ttgcatcatg ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc
    31321 cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc
    31381 atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg
    31441 aaggcgctgg ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt
    31501 tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag
    31561 acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg
    31621 aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca
    31681 actgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc
    31741 gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc
    31801 acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata
    31861 gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc
    31921 tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct
    31981 tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg
    32041 ataacactgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt
    32101 gcacccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg
    32161 tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc
    32221 agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc
    32281 tgcgccatcc ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc
    32341 tggcgcgctt gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac
    32401 cacggttcca gatcgccgct ggctaactgg atatcttttg ggatgccgct ggctaactgg
    32461 atatcttttg ggatatcgac cagaaccgga ccaggacgac ctgagctggc aacgtcgaat
    32521 gcttcagcca tgatgcgcgg caactcttcc agagactgca ccagaaagct gtgcttggta
    32581 caggctaacg acaatcccag gacatccact tcctgaaatg cgtcggtgcc gataaacggt
    32641 gcggacacct gaccagtgat ggcaacgaca gggatggaat ctaacagtgc gtccgcaagc
    32701 ccggttatca ggttggttgc gcccggacca gacgtggcga tacatacgcc agttttaccg
    32761 gtagcacggg cataaccgat agccgctatt gccgcaccct gctcatgtcg gcacagcaag
    32821 tgctccacgc cgccgtcata caatgcatcg taaaccggca taattgcgcc acccggataa
    32881 ccgaaaacgg tgttcacacc ctgtgcccgc aacgcatgta ccacccactg tgcgccattc
    32941 atagttagtt ccccgtcctg aatcttgaga aacagaattt tgtgctgtta ttcattgtct
    33001 gctcctcggt tatgttttta aggtcaaaaa aacccccgga cctttcggtg cgggggtctt
    33061 agttcgttaa ggcttgatct ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg
    33121 ataataatca ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt
    33181 ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa
    33241 tagcacaaga tatttttggg cctattttta tgatggcttt aataatttca atagaaattg
    33301 ttgtttttta aaaagaaaat agtgagtgaa aaaattcaat ttcagtataa atgttgttcc
    33361 cttcctcgta gttcatcctt tgtccgctgc atttttgcga gcatcattcc accgccagaa
    33421 caactcaact tcacttgcac aaataaacag gaatcagcca ctaaaattca cgaattagcg
    33481 ggctacagca gcgatatctt atgcacataa tggtgactca aggagggttt atgtcactgt
    33541 caattgttca tacccgcgca gccctggaag taaatgcgcc cccaatcact gttgaggtac
    33601 atatcagtaa aggtctaccc ggcttaacga tggtgggctt aacagaaaca acggtgaaag
    33661 aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg atatgaatat ccggcgaaaa
    33721 aaatcaccat caaccttgct ccagccgatc tgccaaaaga agggggacga tatgatttac
    33781 ctatcgccat tgcgttgctg gcggcctcag aacagcttac agccaataag ttagatgaat
    33841 atgaattagt cggagaacta gcgcttacag gcgctctgcg tggcgttccc ggtgcaatct
    33901 ccagtgcaac tgaagctatt aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag
    33961 atgaagtggg gctaattaac ggtgaaggat gcctgatagc cgatcatctg caggctgtct
    34021 gtgcgtttct ggaaggtaag aacactctcg aacgcccgaa accaactgat gcagtatccc
    34081 gggcgctaca acatgatctc agtgatgttg tcggtcagga gcaaggaaaa cgaggactgg
    34141 aaattaccgc cgctggtggg cacaaccttt tactgattgg gccgccggga acaggtaaaa
    34201 caatgctcgc cagccgtatt aatggcctgt tgccagattt aagcaatgaa gaggcactgg
    34261 agagcgctgc gatattaagt ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc
    34321 gcccgttccg ctcacctcat cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa
    34381 ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt gctttttctt gatgagctac
    34441 ctgaatttga acggcgtaca ctggatgcct tgcgagagcc gattgaatcc gggcagatcc
    34501 atctttcacg cacacgagca aaaataacct atccagcccg tttccagctt gttgcggcga
    34561 tgaatcccag ccctaccgga cattatcagg gaaaccataa ccgctgcacg ccagaacaga
    34621 cattacgtta tctcaaccgg ctctcggggc cctttctcga ccgcttcgat ctctcactgg
    34681 agatcccatt accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg
    34741 ccaccgttaa acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac
    34801 tgaatgcctg gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg
    34861 cgatgtggtt ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt
    34921 tactgaaagt tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc
    34981 atttgcagga ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac
    35041 tactgacata aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct
    35101 tcagcacctt caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta
    35161 atacgcgtca tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca
    35221 cggcatactg ctacgaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc
    35281 aactcattga aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca
    35341 ccatgacgag agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta
    35401 aagctttccg ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt
    35461 attagttacg cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt
    35521 gcggagagca ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt
    35581 cactttggtc gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc
    35641 agacaactgg aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt
    35701 ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag
    35761 gccgcccgca aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc
    35821 gcctcgttgt gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat
    35881 gcccatacag gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag
    35941 ctgcatgaac gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt
    36001 agtagtcagt tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta
    36061 aagggagatc ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca
    36121 ggtttgatcg gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag
    36181 caacagattg cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa
    36241 ggcggcctgc ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg
    36301 ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat
    36361 gaagtgtatt aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg
    36421 attatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga
    36481 gagactcgaa ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc
    36541 cctaattag
//
LOCUS       DADMFL010000027        36360 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.027, whole genome shotgun sequence.
ACCESSION   DADMFL010000027 DADMFL010000000
VERSION     DADMFL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.027"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNZ27_001373"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNZ27_001373"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY8663766.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="JNZ27_001374"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="JNZ27_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY8663767.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNZ27_001375"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNZ27_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY8663768.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNZ27_001376"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNZ27_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY8663769.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNZ27_001377"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNZ27_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY8663770.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="JNZ27_001378"
     CDS             5281..5739
                     /locus_tag="JNZ27_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY8663771.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCGQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="JNZ27_001379"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="JNZ27_001379"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY8663772.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="JNZ27_001380"
     tRNA            6791..6875
                     /locus_tag="JNZ27_001380"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JNZ27_001381"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JNZ27_001381"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNZ27_001382"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNZ27_001382"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY8663773.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNZ27_001383"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNZ27_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY8663774.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNZ27_001384"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNZ27_001384"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8663775.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JNZ27_001385"
     CDS             complement(10156..13899)
                     /locus_tag="JNZ27_001385"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY8663776.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNZ27_001386"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNZ27_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY8663777.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JNZ27_001387"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JNZ27_001387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY8663778.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JNZ27_001388"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JNZ27_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY8663779.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNZ27_001389"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNZ27_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY8663780.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWES"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JNZ27_001390"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JNZ27_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY8663781.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /locus_tag="JNZ27_001391"
     CDS             complement(20438..21133)
                     /locus_tag="JNZ27_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8663782.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNZ27_001392"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNZ27_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY8663783.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEVFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JNZ27_001393"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JNZ27_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY8663784.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23296..23403
                     /locus_tag="JNZ27_001394"
     CDS             23296..23403
                     /locus_tag="JNZ27_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY8663785.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(23552..24406)
                     /gene="kdsA"
                     /locus_tag="JNZ27_001395"
     CDS             complement(23552..24406)
                     /gene="kdsA"
                     /locus_tag="JNZ27_001395"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY8663786.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24442..25251)
                     /locus_tag="JNZ27_001396"
     CDS             complement(24442..25251)
                     /locus_tag="JNZ27_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY8663787.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25255..25647)
                     /locus_tag="JNZ27_001397"
     CDS             complement(25255..25647)
                     /locus_tag="JNZ27_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY8663788.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25644..26477)
                     /gene="prmC"
                     /locus_tag="JNZ27_001398"
     CDS             complement(25644..26477)
                     /gene="prmC"
                     /locus_tag="JNZ27_001398"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8663789.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26477..27559)
                     /gene="prfA"
                     /locus_tag="JNZ27_001399"
     CDS             complement(26477..27559)
                     /gene="prfA"
                     /locus_tag="JNZ27_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY8663790.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAEQPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27601..28857)
                     /gene="hemA"
                     /locus_tag="JNZ27_001400"
     CDS             complement(27601..28857)
                     /gene="hemA"
                     /locus_tag="JNZ27_001400"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY8663791.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29071..29694
                     /gene="lolB"
                     /locus_tag="JNZ27_001401"
     CDS             29071..29694
                     /gene="lolB"
                     /locus_tag="JNZ27_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY8663792.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29694..30545
                     /gene="ispE"
                     /locus_tag="JNZ27_001402"
     CDS             29694..30545
                     /gene="ispE"
                     /locus_tag="JNZ27_001402"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY8663793.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30696..31643
                     /locus_tag="JNZ27_001403"
     CDS             30696..31643
                     /locus_tag="JNZ27_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY8663794.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31768..33447
                     /gene="dauA"
                     /locus_tag="JNZ27_001404"
     CDS             31768..33447
                     /gene="dauA"
                     /locus_tag="JNZ27_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY8663795.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33502..33780)
                     /gene="ychH"
                     /locus_tag="JNZ27_001405"
     CDS             complement(33502..33780)
                     /gene="ychH"
                     /locus_tag="JNZ27_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY8663796.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34058..34642
                     /gene="pth"
                     /locus_tag="JNZ27_001406"
     CDS             34058..34642
                     /gene="pth"
                     /locus_tag="JNZ27_001406"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8663797.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34759..35850
                     /gene="ychF"
                     /locus_tag="JNZ27_001407"
     CDS             34759..35850
                     /gene="ychF"
                     /locus_tag="JNZ27_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY8663798.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         8977 a         9453 c         8898 g         9032 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgagcgta
     5521 tttgaacatt gtgggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagctggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg catcgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cagtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcataacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacgattc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacac ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgcaaaat gttgtcatta
    23281 caacaaggcg gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca
    23341 gcaccgatcc tggcggggat tattaccgca gcgattgtca gctggtggcg taaccggaag
    23401 taaggtgaaa acgggcgtca ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag
    23461 gggtaacagc ctgcggcctt ttttgtatct ggcacccatg aaaaaagtct taacgcagaa
    23521 cgctaagact ttatttttca agcaaaaaag attacttgct ggtatccagt tcttcgaaac
    23581 ctttcaccag atcatcaatc gctttcatct gcttgaggaa cggttccagt ttagccagcg
    23641 gcagcgcgga tggaccatca catttcgcat gttccggatc cggatgcgct tcaataaaca
    23701 gccccgccag acctaccgcc ataccggctc gtgccagctc agccacctga gcacgacgac
    23761 caccggaagc tgcgccaaac ggatcgcggc attgcagtgc gtgggtcacg tcgaaaatca
    23821 ccggcgagtt accagacact ttcttcataa tgctgaagcc cagcatatcg acaaccaggt
    23881 tgtcatagcc gaagttagca ccacgatcgc aaagaatcac tttttcgttg ccgccttctt
    23941 tgaatttatc aacgatatta cccatctgtc ccgggctgac aaactgtggt ttcttgacgt
    24001 taattaccgc accggttttc gccatggctt caaccaggtc agtctggcga gcaagaaacg
    24061 ccggcaactg aatcacatcc acgacatcag caacgggctg tgcctgactt ggttcgtgaa
    24121 cgtcggtgat aattttcacg ccaaaagtct gcttcaactc ctggaagatt ttcatccctt
    24181 cttccaggcc cggtccacga taagagtgga tggaggagcg gttggctttg tcaaaagagg
    24241 ctttgaacac gtaagggata cccagtttct gggtcacagt tacgtagtgc tcgcaaatgc
    24301 gcatcgccag atcgcgagat tccagcacgt tcataccgcc aaacagtacg aacggcaggt
    24361 catttgctac gttgatgtcg ccaatgctaa ccactttttg tttcatagga tcgccttaat
    24421 cttgagtaaa atgtcgatta attaatgcag cacaatatgt ttatgcgcga tgttatttat
    24481 ttgcgcacgg atcatttcgc tgatcgggtc ttccggacac tgttcaacga aataacttaa
    24541 atcgttcaac gcaacgtgtt cgcaatccag ttgcgcataa atcaacccgc gatcgcgaat
    24601 ttcataggga tcttcagggt tgaattgtaa taaagcttcg ctggtgcgta acgccagctc
    24661 catctgattt tcttccatca acgaggcttt gagtgtatcc agcaatttgc ggattacctc
    24721 aatgttatca gcttcatcaa ggtcttcata aaacagttcc gccgacgggc tgatatttcc
    24781 ctttaaccat acgtccagca tatgttcgct taacgattca ccgttaaaag gattaatcag
    24841 ccaaatttcg ccatccggac attcaatgcg caatatcagc tgcgtaggga aaatcaccgg
    24901 cagcagcggc aaatcgagac gattcgcgac ccataataaa accgcaccta atgataccgc
    24961 actgccctgt cgattcttta acacctggtc cagccataat gcatcggaaa gacgataaac
    25021 gccgcgtgag gctttaaatc cccagtcgcc gtaaaacagc gcgatcagtt tttccagctg
    25081 ctcttctaaa ggcagaagct ggctgatttc ttccttcgcc agactaacga gacgctccag
    25141 ttcgtcgtaa acatcttgcg agggaaaatc gcggcggatt gcttcgcaag ccaggatcat
    25201 gccttcgcac aatggcgctt tattaaattc gaaatcagct aacgatctca tgacttaccc
    25261 cagtaacggt acttttgtgg tggcgagttt aatgatgatg tacagcacca ccaacgccag
    25321 cgggaaggca ataatgcgcg cctgctgact gtgcatacga cgataatcga gtgcaataaa
    25381 acccaaaacg atataaatga taactccaaa cagcttttca gtcagccact gtgcctgccc
    25441 ggaaaatggc aggatgtgcg ctttagccat caacgcaatg ccgcttaaca ataacaccgt
    25501 atcgacaacc ggcggcacaa tgcgcgtcca gcgagcaaat gcctgtggat gcttctgata
    25561 acgtagccag aaacgtaagg ttaatagccc aacagaaagc gcgatactaa taagatgaac
    25621 actaagcagt gtagaaaaac ttgtcattga taatagcggc cgagcgttac gcgctcgtta
    25681 tcaccatagt cacggcaggt ttcgacgtca tgataccccg cgaggataaa tgcttgtcgc
    25741 accgcttcgc cctgctgcca gccatgttcc agaagcagaa agccgccgga taccagcgcg
    25801 ttacgcgact gttcgatgat atgcacgatg tctgccattc cactgtctgc cgcaaccagc
    25861 gcagtgagcg gctcaaagcg gacatcgcct tgttgaagat gtgggtcctg ctcgtcaata
    25921 tacggcggat tgctgacaat catcgcaaac tgctgcccgg ctagcgcgct aaaccagtcg
    25981 ctttgcagaa tgtggatatt tttgatcgcc agatgctggg cattacgttg tgccagggag
    26041 acagcatcag gcatacgatc tacagcgata atttcgcagt ccgggcgctc gctagccagc
    26101 gcaagcgcaa tcgccccggt acccgtcccg agatcgagaa tacggcaagg ttgttcaggc
    26161 aaccgcgcca gtgcctgctc caccagacac tccgtatccg ggcgcggaat taaggtcgct
    26221 ggagaaacaa ataacggcaa cgaccagaat tctcgcaccc cggttaaatg agcaatgggt
    26281 tcaccatcgc gacgacgtgt cagtagcgca tcaagttgct gacattgttc gtcagtcagc
    26341 tgcgtttcac caaaggcgag aataaaagta cgccctctgc cggtaacatg ctccagcagg
    26401 atttcagcat cacgccgcgg gctttcgctc gcctgaagtt ggcttattgc ttcacgtaac
    26461 cagtgttgat attccattat tcctgctcgg acaacgccgc cagttggtcg gcctgatgtt
    26521 cctggataat cggttcaatc agcatatcca gcttaccttc catcacttca tccaggcggt
    26581 agagcgtcag gttgatgcgg tgatcggtaa cgcgcccctg cgggaaattg taagtacggt
    26641 tacggtcgct gcgatcgcca ctccccagca ggttacgacg ggtagacgct tcggcctgtt
    26701 ggcgttttgc catttcagca gcgtggatgc gagcaccgag cactgaaagc gccttcgctt
    26761 tgtttttatg ttgtgaacgt tcgtcctgac attcaacaac aatcccggtc ggcaagtgag
    26821 taatacgaat tgccgaatcg gtggtgttaa cgtgctgacc acccgcccct gacgagcgga
    26881 aagtatcaat gcgtaaatct gctgggttga tgtccggctg ttctgcgtca ggcagttctg
    26941 gcattaccgc aacggtacaa gcagaagtat gaatacgacc ctgcgattcc gtagcaggaa
    27001 cacgttgcac gcgatgaccg ccggattcaa atttcagacg accatacaca ccatcaccgc
    27061 taattttggc gatgatctct ttataaccac catgttcacc ctcgctggcg ctcatgattt
    27121 ctacccgcca gcggcgggct tcggcgtaac ggctgtacat acggaacaga tcgcctgcaa
    27181 acagcgctgc ttcgtcgccg ccggttccgg cacggacttc gaggaaggcg ttacgttcgt
    27241 catcaggatc ttttggtagt aatagaacct gtaattgctg ttccagttgc tcgctttttt
    27301 ctttagcttc gcgcagttca tcctgcgcca tctcacgcat ttcaggatcg tcgagcatca
    27361 tctgtgcggt ttcgatatct tcctgaacct gttgccagtc ggtaaaacag cgcgaaacat
    27421 cacttaactg cgcatattcg cgtgataatg cgcgaaaacg ttcctggtcg gcgatagttt
    27481 gcgcgtcacc cagcaacgcc tgaacttctt catggcgttc atgcagggct tccagtttgg
    27541 caacgataga aggcttcata ggcgtaaatg caccctgtaa aaaaagaaaa tgatgtactg
    27601 ctactccagc ccgaggctgt cgcgcagaat attcaggcgt tcgttatccc cgtcacgggc
    27661 ggcctgttga agtgatttcg ttggcgcatg gatcaagcgg ttagtcagtt tccatgccag
    27721 atcctgcata atggcttgcg cgtcgccgcc ctgctcaagg gccgctaacg ctttggcggt
    27781 taactcatcg cgaacgtgct ctgcctggct gcgatactcg cgaatggttt cgctggcgct
    27841 ttgtgctcgc agccacgcca taaattcgct ggtttcctga gcgacaatag tttccgcctc
    27901 aaccgctgcg gctttacgct gcgccaggtt gtgcgaaatg atgctttgca gatcgtccac
    27961 actataaaga taagcattcg ccagtttgcc aacttccggc tcaacatcgc gcggaacggc
    28021 aatatccacc aacagcattg gttggttacg acgacttttt aatgcgcgct ccaccatgcc
    28081 tttcccgata atcggtaacg ggctggcggt ggaactgatg atgatatcgg cttcgcgcag
    28141 acgttcgtcg atctcactca gggcaatcac ttccgcgcca acttcatctg ccagaatttg
    28201 ggcacgttcg cgagtgcggt tggcgataat catcttctgt actttatgtt cgcgcagatg
    28261 acgcgctacc agctcgatgg tttcgcccgc gcctaccagc aacactgtga ccgtagagag
    28321 cgattcaaag atctgccgcg ccagcgtaca agccgcaaaa gcgacagaca cagcactggc
    28381 accgatatct gtttcagtgc gaacgcgttt cgctacagag aaagatttct ggaacatgcg
    28441 ttccagttcg ctggccttca tatgaccttt ttgcgaatcg gcaaacgctt ttttaacctg
    28501 accgaggatc tgcggctccc caagaaccag tgaatccagg ccgctggcaa cacgcattaa
    28561 atggctaacc gcgtcgttat cctgatgcca gtagaggttt ttacgcagat cttcttcatt
    28621 aagattgtga taatcgcaaa gccagcggat taacgcctct tgcaggttat cctgctcttc
    28681 aacgctaaga taaagttccg tgcggttgca cgtcgacagc accacgccgc cctgcaccat
    28741 cggctgcgca agcaggctgt caagcgcctg atcgagctta tccggcgaaa acgatacacg
    28801 ttctcgcagc gatacaggtg ccgttttatg gttgataccg agtgctaaaa gggtcatgtc
    28861 tgcgggaaat aataccaacg ttgatagggt tagtctgctt gcatcataca ggatgcgtaa
    28921 gatcaataaa agagagcgcc cccttttgga gtaattgccg aaagccgtta gattctggca
    28981 attaagacaa cttgaacata gacgatagcg gacggtaacg ctagcattaa gggttataac
    29041 tgcaacgtat ctcaaggact tgtcatcact atgcccctgc ccgattttcg tcttatccgc
    29101 ctgctaccgc tggctgctct tgtgctcact gcctgttccg ttaccacgcc caaaggtcct
    29161 ggcaaaagcc cggattcgcc acaatggcgt cagcatcagc aagacgtgcg caatcttaat
    29221 cagtatcaga ctcgcggcgc gttcgcttat atttctgacc aacaaaaagt gtacgcccgc
    29281 tttttctggc agcaaaccgg ccaggatcgc taccgtctgc tgctcactaa cccattgggc
    29341 agcacggaac tggagctgaa tgctcaaccg ggtaacgtgc agttagtcga caataaaggt
    29401 cagcgttata ccgccgatga cgccgaagag atgattggca aattgaccgg aatgccaatt
    29461 ccgctcaaca gcttgcgcca gtggatttta ggtttaccgg gtgatgcaac cgactacaaa
    29521 ctggacgacc agtaccgcct gagcgaaatt acctacagcc agaatggcaa aaactggaag
    29581 gttgtttatg gtggttatga caccaaaacg caacctgcga tgccagccaa tatggaactc
    29641 accgacggtg gtcaacgcat caagttaaaa atggataact ggatagtgaa ataatgcgga
    29701 cacagtggcc ctctccggca aaacttaatc tgtttttata cattaccggt cagcgtgcgg
    29761 atggttacca cacgctgcaa acgctgtttc agtttcttga ttacggcgac accatcagca
    29821 ttgagcttcg tgacgatggg gatattcgtc tgttaacgcc cgttgaaggc gtggaacatg
    29881 aagataacct gatcgttcgc gcagcgcggt tgttgatgaa aactgcggca gacagcgggc
    29941 gtcttccgac gggaagcggt gcgaatatca gcattgacaa gcgtttgccg atgggcggcg
    30001 gtctcggcgg tggttcatcc aatgccgcga cggtcctggt ggcattaaat catctctggc
    30061 aatgcgggct aagcatggat gagctggcgg aaatggggct gacgctgggc gcagatgttc
    30121 ctgtctttgt tcgggggcat gccgcgtttg ccgaaggcgt tggtgaaata ctaacgccgg
    30181 tggatccgcc agagaagtgg tatctggtgg cgcaccctgg tgtaagtatt ccgactccgg
    30241 tgatttttaa agatcctgaa ctcccgcgca atacgccaaa aaggtcaata gaaacgttgc
    30301 taaaatgtga attcagcaat gattgcgagg ttatcgcaag aaaacgtttt cgcgaggttg
    30361 atgcggtgct ttcctggctg ttaaaatacg ccccgtcgcg cctgactggg acaggggcct
    30421 gtgtctttgc tgaatttgat acagagtctg aagcccgcca ggtgctagag caagccccgg
    30481 aatggctcaa tggctttgtg gcgaaaggcg ttaatctttc cccattgcac agagccatgc
    30541 tttaagccgg gcaagctgag tttcggtgac aacgtcacct tgttccagac gttgcatcgc
    30601 gctctttaat acaccgcctg gaaaggatca tgcctggccc gcacagtttt cggcagattc
    30661 tttccaccaa tggacgcatg cctgaggttc ttctcgtgcc tgatatgaag ctttttgctg
    30721 gtaacgccac cccggtacta gcacaacgta ttgccaaccg cctgtacact tcactcggcg
    30781 acgccgctgt aggtcgcttt agcgacggcg aagtcagcgt acaaattaac gaaaatgtac
    30841 gcggtggtga tattttcatc atccagtcca cttgtgcccc tactaacgac aacctgatgg
    30901 aattagtcgt tatggttgat gccctgcgtc gtgcttccgc aggtcgtatc accgctgtta
    30961 tcccctactt tggctatgcg cgccaggacc gtcgcgtccg ttccgctcgt gtaccaatca
    31021 ctgcgaaagt ggttgcagac ttcctctcca gcgtcggtgt tgaccgtgtg ctgacagtgg
    31081 atctgcacgc tgaacagatt cagggtttct tcgacgttcc ggttgataac gtatttggta
    31141 gcccgatcct gctggaagac atgctgcagc tgaatctgga taacccaatt gtggtttctc
    31201 cggacatcgg cggcgttgtg cgtgcccgcg ctatcgctaa gctgctgaac gataccgata
    31261 tggcaatcat cgacaaacgt cgtccgcgtg cgaacgtttc ccaggtgatg catatcatcg
    31321 gtgacgttgc aggtcgtgac tgcgtactgg tcgatgatat gatcgacact ggcggtacgc
    31381 tgtgtaaagc tgctgaagcg ctgaaagaac gtggtgctaa acgtgtattt gcgtacgcga
    31441 ctcacccgat cttctctggc aacgcggcga acaacctgcg taactctgta attgatgaag
    31501 tcgttgtctg cgataccatt ccgctgagcg atgaaatcaa atcactgccg aacgtgcgta
    31561 ctctgaccct gtcaggtatg ctggccgaag cgattcgtcg tatcagcaac gaagaatcga
    31621 tctctgccat gttcgaacac taatcgaacc cggctcaaag acccgctgcg gcgggttttt
    31681 ttgtctgtaa tatccatttg tatgacctat gcctccttca cctgccattt agttgacaga
    31741 tgatgcgctc acggatgaaa cattattgtg aacaaaatat tttcctcaca tgtgatgcct
    31801 ttccgcgctc tgatcgacgc ttgctggaaa gaaaaatata ctgccgcacg gtttacccgt
    31861 gacctgattg ccgggataac cgtcgggatt attgctatcc cgctggcgat ggcgttggct
    31921 attggtagtg gtgtggcacc ccagtacggt ttatataccg cagctgttgc ggggattgtc
    31981 attgctctaa cgggtgggtc acgctttagc gtttccggtc cgactgcggc atttgtggta
    32041 attctctatc ccgtttcgca acagtttgga ctggcaggac tgctggttgc gaccttgttg
    32101 tcgggaatct ttttgattct gatggggctg gcacgctttg gtcgtctgat tgagtatatt
    32161 ccggtttccg tcaccttagg tttcacctcg ggtatcggga tcaccatcgg taccatgcag
    32221 attaaagatt ttctcggtct gcaaatggcc catgtcccgg aacattatct acaaaaagtc
    32281 ggcgcattat ttatggcgct gccgaccatt aatgtgggtg atgctgccat tggcattgtg
    32341 acgcttggca ttctggtttt ctggccgcgt ctgggcattc gtttacccgg tcaccttccg
    32401 gccttgctgg ctggttgcgc ggtgatgggg attgttaacc tgctcggcgg acatgttgct
    32461 accatcggtt cgcaattcca ctacgtcctg gccgatggtt ctcagggtaa cggtattccg
    32521 caactgctgc cacaactggt gctgccgtgg gatctgccta attcagaatt cacgctaacc
    32581 tgggattcta ttcgcaccct gctgcctgcg gcattctcaa tggcaatgct cggggcgatc
    32641 gaatctctgc tctgcgctgt ggtactggat ggtatgaccg ggactaagca caaggcgaat
    32701 agtgaactgg tcggtcaggg gctggggaat atcatcgctc cattctttgg tggtattacc
    32761 gctactgctg ccatcgcgcg ttctgccgct aacgtccgtg ccggggcaac ttcccctatc
    32821 tcggcggtga tccactctat tctggttatt ctcgccctgc tggtactggc accactgctc
    32881 tcctggctgc cgctttccgc catggcagcc ctgctgttga tggtggcgtg gaacatgagt
    32941 gaagcgcaca aagtggtcga cttgctgcgt catgcgccga aagatgacat catcgtcatg
    33001 ctgctgtgta tgtcactgac cgtgctgttt gatatggtta ttgccatcag cgtggggatc
    33061 gtgctggcat cgctgctgtt tatgcgtcgt atcgcacgta tgactcgcct ggcaccggta
    33121 gtcgtagatg ttccagacga tgtcctggtt ctgcgcgtta ttggcccgct gttttttgct
    33181 gctgctgaag gcttattcac ggacctggag tcacgtcttg aaggcaaacg gattgtgatt
    33241 ctgaagtggg atgccgttcc ggtacttgat gctggtggtc ttgatgcgtt ccagcgtttt
    33301 gtgaagcgtc tgcccgaagg atgtgaactg cgcgtgtgca acgtggaatt ccagccactg
    33361 cgaactatgg ctcgcgcagg cattcaaccg atcccgggac gcctcgcgtt cttcccgaat
    33421 cgtcgcgcgg cgatggcgga tttataagta cgagattgac cagtcagcgc aaactgactg
    33481 gtcagcaaac tgcattattt gttagctatg acggcgatta tcgctacggc ggcaacgttt
    33541 gtcatagtga cgaacccacc agtaacggtc gcacacctgt tcatgcccgc caacacgggc
    33601 acccgccagc cagagaatgg caccgacgaa aatacttagc actgcaccat gtgcgaaata
    33661 ctggggcata ttaaactgtg gtaactggtt gaggattgaa taccccacgc cgaccaccat
    33721 taccaccaga cccaacccca tgagcacgtt accgagtatc gaagcgtttt tgcgtttcat
    33781 atgtcacctc cggaactttc tgggttgtaa cagggaatac ccctcttcct tatgtgtaaa
    33841 gtatagacaa caccctgtgg gttatgtgcg ggcgtaatca caattacaac ccttatttca
    33901 acaaaacttt acaaataaac gcctgaacac cttgactttc taatagtcca ctcaggcaat
    33961 tattacgtta tctgcatttg atacgcagtt ttttgtcaag cggggccgca accagtaaac
    34021 tacgcgccag ttatgtacac actcaggaca aaaaaacgtg acgattaaat tgattgtcgg
    34081 cctggcgaac cccggtgctg aatacgccgc aacgcgacat aatgctggtg cctggttcgt
    34141 tgacttactg gcagagcgtt tgcgcgctcc gctgcgcgaa gaggctaaat tctttggtta
    34201 tacttcgcga gtcacccttg gaggcgaaga tgtccgcctg ttagtcccga ctacatttat
    34261 gaatctcagc ggcaaagccg ttgcggcgat ggccagtttt ttccgcatta atccggacga
    34321 aattctggtg gcccacgacg aactggatct gcctcctggc gtcgccaaat ttaagttggg
    34381 cggtggccat ggtggtcaca atggactgaa agacatcatc agtaaattgg gtaataaccc
    34441 taactttcac cgtttacgca tcggaatcgg tcatccgggc gataaaaata aagttgtcgg
    34501 ttttgtgtta ggcaaaccgc ctgttagtga acagaagtta attgatgaag ccatcgacga
    34561 agcggcgcgt tgtactgaaa tgtggtttac agatggcttg accaaagcaa cgaaccgatt
    34621 gcacgccttt aaagcgcaat aagtcgttgt ctgcggcatt tttgccgagt gccgtgtata
    34681 ataggcaaag ttatttccat ttctgcaatc tgttagcaat aacaggttga ttattaagat
    34741 attaaggtga tttaaatcat gggattcaaa tgcggtatcg tcggtttgcc caacgtcggg
    34801 aaatctaccc tgttcaacgc gctgaccaaa gccggtattg aagcggccaa ctttccattc
    34861 tgcaccattg agccgaacac aggcgtcgta ccaatgcctg accctcgcct ggatcaactg
    34921 gctgaaatcg taaaaccgca acgtacgctt cccacgacca tggaatttgt cgatatcgcc
    34981 ggtctggtaa aaggcgcatc gaaaggcgaa ggtctgggta accagttcct gaccaacatc
    35041 cgtgaaaccg aagcgatcgg tcacgttgtt cgctgctttg aaaatgacaa catcattcac
    35101 gtttccggca aagttaaccc ggctgacgat attgaagtta tcaacaccga actggcgctg
    35161 gcagacctcg acacctgcga acgtgcgatt catcgcgtac agaagaaagc caaaggtggc
    35221 gataaagacg cgaaagctga gctggcggtc ctggaaaaat gcctgcccca gctggaaaac
    35281 gcaggtatgc tgcgcgcgct ggatttaagc gctgaagaga aagcggctat tcgttacctg
    35341 agcttcctga cgctgaaacc aacaatgtac atagccaacg tcaacgaaga cggttttgaa
    35401 aacaacccat atcttgacca ggtgcgtgaa atcgcggcga aagaaggttc tgttgtggtt
    35461 ccggtttgtg ctgctgttga agcagacatt gccgaactgg acgacgaaga acgtgacgag
    35521 tttatgcagg agcttgggct ggaagaaccg ggcctgaacc gtgtgatccg tgccggttat
    35581 aagctgctga acctgcaaac ttacttcacc gctggggtga aagaagtgcg tgcatggacc
    35641 attccggttg gagcaaccgc gccgcaggca gcgggcaaaa tccatactga ttttgaaaaa
    35701 ggctttatcc gtgcacaaac catctcgttt gaagatttca tcacttacaa aggtgaacaa
    35761 ggcgcgaaag aagcaggcaa aatgcgtgca gaaggtaaag attacatcgt gaaagatggc
    35821 gatgtgatga acttcctttt caacgtctaa ttaatttatt gtctcatgag gtttcgctaa
    35881 atctcatgaa gagcgcaaaa gtatataaaa tccacgcaat tgcgtggatt tttcatttca
    35941 ccgagtccta atcattcttg taacatccta cacttttgtg cttattgcct acagtgaacc
    36001 acgagaataa ttaaagacat aagtgttcag atgactgata tctcattagc agtgatataa
    36061 cgtcagtttt tgttccacta cacatcagcc ctcttcacag ctgatcaata ttgatctcgc
    36121 tccaatatgc tggcgagaaa aggacttacc tgaagggtaa tatcccgcag ataaagtccc
    36181 cgaataacgt gattacgtgc gatcctggca agatgaaaat gataaccagt agcacaataa
    36241 taacgatagg ccagaaaatc cattgaccaa aaatgaatat cgatgttttt tgctttttct
    36301 tattagtaat gcaaaaatgg cgcatattgt ttttgttatg cagatacatc aagcgaaaat
//
LOCUS       DADMFL010000028        35995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.028, whole genome shotgun sequence.
ACCESSION   DADMFL010000028 DADMFL010000000
VERSION     DADMFL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.028"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="JNZ27_001408"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="JNZ27_001408"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY8663799.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RALLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPLGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /locus_tag="JNZ27_001409"
     CDS             2619..3380
                     /locus_tag="JNZ27_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8663800.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="JNZ27_001410"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="JNZ27_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY8663801.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="JNZ27_001411"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="JNZ27_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY8663802.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="JNZ27_001412"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="JNZ27_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY8663803.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="JNZ27_001413"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="JNZ27_001413"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY8663804.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFTCYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILSSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNZ27_001414"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="JNZ27_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY8663805.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNZ27_001415"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="JNZ27_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY8663806.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="JNZ27_001416"
     CDS             complement(10310..11188)
                     /locus_tag="JNZ27_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663807.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNZ27_001417"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="JNZ27_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY8663808.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="JNZ27_001418"
     CDS             13815..14150
                     /locus_tag="JNZ27_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY8663809.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="JNZ27_001419"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="JNZ27_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY8663810.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15503
                     /locus_tag="JNZ27_001420"
                     /pseudo
     CDS             14884..15503
                     /locus_tag="JNZ27_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            15528..16544
                     /gene="phnD"
                     /locus_tag="JNZ27_001421"
     CDS             15528..16544
                     /gene="phnD"
                     /locus_tag="JNZ27_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8663811.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16650..17429
                     /gene="phnE"
                     /locus_tag="JNZ27_001422"
     CDS             16650..17429
                     /gene="phnE"
                     /locus_tag="JNZ27_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY8663812.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17450..18168
                     /gene="phnF"
                     /locus_tag="JNZ27_001423"
                     /pseudo
     CDS             17450..18168
                     /gene="phnF"
                     /locus_tag="JNZ27_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
     gene            18169..18621
                     /gene="phnG"
                     /locus_tag="JNZ27_001424"
     CDS             18169..18621
                     /gene="phnG"
                     /locus_tag="JNZ27_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY8663813.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18618..19202
                     /gene="phnH"
                     /locus_tag="JNZ27_001425"
     CDS             18618..19202
                     /gene="phnH"
                     /locus_tag="JNZ27_001425"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY8663814.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19202..20266
                     /gene="phnI"
                     /locus_tag="JNZ27_001426"
     CDS             19202..20266
                     /gene="phnI"
                     /locus_tag="JNZ27_001426"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY8663815.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20259..21104
                     /gene="phnJ"
                     /locus_tag="JNZ27_001427"
     CDS             20259..21104
                     /gene="phnJ"
                     /locus_tag="JNZ27_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY8663816.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21101..21859
                     /gene="phnK"
                     /locus_tag="JNZ27_001428"
     CDS             21101..21859
                     /gene="phnK"
                     /locus_tag="JNZ27_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY8663817.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            21970..22650
                     /gene="phnL"
                     /locus_tag="JNZ27_001429"
     CDS             21970..22650
                     /gene="phnL"
                     /locus_tag="JNZ27_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY8663818.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22647..23783
                     /gene="phnM"
                     /locus_tag="JNZ27_001430"
     CDS             22647..23783
                     /gene="phnM"
                     /locus_tag="JNZ27_001430"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY8663819.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVTSGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23783..24340
                     /gene="phnN"
                     /locus_tag="JNZ27_001431"
     CDS             23783..24340
                     /gene="phnN"
                     /locus_tag="JNZ27_001431"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY8663820.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24327..24761
                     /gene="phnO"
                     /locus_tag="JNZ27_001432"
     CDS             24327..24761
                     /gene="phnO"
                     /locus_tag="JNZ27_001432"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY8663821.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24763..25521
                     /gene="phnP"
                     /locus_tag="JNZ27_001433"
                     /pseudo
     CDS             24763..25521
                     /gene="phnP"
                     /locus_tag="JNZ27_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25668..25997)
                     /locus_tag="JNZ27_001434"
     CDS             complement(25668..25997)
                     /locus_tag="JNZ27_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663822.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26066..26698)
                     /locus_tag="JNZ27_001435"
     CDS             complement(26066..26698)
                     /locus_tag="JNZ27_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8663823.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26779..28994)
                     /locus_tag="JNZ27_001436"
                     /pseudo
     CDS             complement(26779..28994)
                     /locus_tag="JNZ27_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29108..30628
                     /locus_tag="JNZ27_001437"
     CDS             29108..30628
                     /locus_tag="JNZ27_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8663824.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQSSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMACKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30625..31599
                     /locus_tag="JNZ27_001438"
     CDS             30625..31599
                     /locus_tag="JNZ27_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY8663825.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31630..32571
                     /locus_tag="JNZ27_001439"
     CDS             31630..32571
                     /locus_tag="JNZ27_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8663826.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32626..33309
                     /locus_tag="JNZ27_001440"
     CDS             32626..33309
                     /locus_tag="JNZ27_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY8663827.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33324..34184
                     /locus_tag="JNZ27_001441"
     CDS             33324..34184
                     /locus_tag="JNZ27_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY8663828.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34181..35182
                     /locus_tag="JNZ27_001442"
     CDS             34181..35182
                     /locus_tag="JNZ27_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY8663829.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35179..35885)
                     /locus_tag="JNZ27_001443"
                     /pseudo
     CDS             complement(35179..35885)
                     /locus_tag="JNZ27_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         8367 a        10068 c         9583 g         7977 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggctct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ctaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggccatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tacctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tctcctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaagcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgact ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg catcgtgatg gacaccctgc
    15301 gcgacatcaa ccagaacgac ggcatcaccg tggtcgtcac gctgcatcag gtggattacg
    15361 ccctgcgcta ctgcgaacgc atcgtcgccc tgcgtcaggg gcacgtcttc tacgacggca
    15421 gcagccaact gtttgataac gaacgttttg accatctcta ccgcagcatt aaccgcatcg
    15481 aagagaacgc gaaagctgcc tgatatcccc atcattgagg aaaacgaatg aacgctaaga
    15541 taattgcctc gctggccttc accagcatgt tcagcctcag caccctgtta accccggcac
    15601 acgccgaaga gcaggaaaag gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa
    15661 acctgaaacc gcaatggacg ccgtttttac aggatatgga gaagaaactg ggcgtgaagg
    15721 tcaacgcctt ctttgccccg gactacgcgg gcattatcca ggggatgcgc ttcaataaag
    15781 tggatattgc ctggtacggc aacctgtcgg cgatggaagc ggtggatcgc gccaatggcc
    15841 aggtcttcgc ccagacggtc gcggcggatg gatcgccggg ttactggagc gtgttgatcg
    15901 tcaacaaaga cagcccgatc aacaacctga acgatctgct ggcgaagcgg aaagatctca
    15961 ccttcggcaa tggcgatcct aactccacct ctggcttcct cgtccccggt tactacgtct
    16021 tcgctaaaaa caatatctcc gccagcgact tcaagcgcac cgtcaacgcc gggcatgaaa
    16081 ccaacgcgct ggccgtcgcc aacaagcagg tggatgttgc caccaacaac accgaaaacc
    16141 tcgacaagct gaaaacctcc gcgccggaga agcttaaaga gctgaaggtg atctggaaat
    16201 cgccgctgat cctaggcgat ccgatcgtct ggcgcaagaa tctgtccgaa actaccaaag
    16261 acaagatcta cgacttcttt atgaactacg gaaaaacgcc ggaagagaaa gcggtgctgg
    16321 aacgcctggg ctgggcgccg ttccgcgctt ccagcgacct gcaactggtg ccgattcgcc
    16381 agctcgcact gtttaaagag atacaaagcg tgaaaggcaa taaagggctg aatgagcagg
    16441 acaagctggc gaaaaccacc gagattcagg cgcagctgga tgacctggac cgcctgaaca
    16501 acgcgttaag cgcgatgagt tcggtgagta aagcggtgca gtaaatcgta ggtcggataa
    16561 gacgccccgg cgtcgcatcc gacaatgtgc aggcgttgat gccggatgcg gtgcaagcac
    16621 cttatccggc ctacagaccg gagccaaaca tgcaaaccat caccatcgcc ccacccaagc
    16681 gtagctggtt ctcgcttctg agctgggccg ttgttctcgc cgtgctggtc gtctcgtggc
    16741 agggcgcgga aatggccccg cttacgctga ttaaagacgg cggcaacatg gcaaccttcg
    16801 ctgccgactt cttcccgccc gatttcagcc agtggcagga ttacctcacc gaaatggccg
    16861 tcacgctgca aatcgccgtc tggggcaccg cgctggcagt ggttctctcc atccccttcg
    16921 gtctgatgag cgccgaaaac ttggtgccgt ggtgggttta ccagcccgtt cgccgcctga
    16981 tggacgcctg ccgcgccatt aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg
    17041 gcctcggccc gttcgctggc gtgctggcgc tgtttatcca caccaccggc gtgctctcca
    17101 agctgctttc cgaagcggag gaagcgatcg aacccggccc ggtggaaggc attcgcgcca
    17161 ccggcgccaa caaactcgaa gagatccttt acggcgtgct gccgcaggtg atgccgctgc
    17221 tgatctccta ctccctctat cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca
    17281 tggtcggcgc gggcgggatc ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc
    17341 agcaaacctg cgccctgatg gtgcttatca tcgtcacggt cagcctgctg gatttcctct
    17401 ctcaacggtt gcgtaagcac tttatctgat aagcgaggca ttgatatcta tgcacttgtc
    17461 tacacatccg accagctacc caacacgcta tcaggagata gccgcaaaac ttgagcagga
    17521 gcttcgtcaa cactaccgct gcggcgacta tcttcccgcc gagcagcaac tggcggcgcg
    17581 ctttgaggtg aaccgccaca ccctgcgccg cgccatcgac caactggtgg aaaaaggctg
    17641 ggtacagcgc cgtcagggcg tcggcgtgct ggtgctgatg cgcccgttcg attacccgct
    17701 caacgcccag gcgcgtttta gccagaacct gctggaccag ggcagccatc ccaccagcga
    17761 aaaactgctg tcggtattgc gtccggtcac gttgctgacg cgctggggat taccgagggc
    17821 gagaacgtta tccatttgcg caccctgcgc cgggtcaacg gcgtcgcgct ctgtttaatc
    17881 gaccactact ttgcggacct caccctttgg ccgacgctgc aacgcttcga cagcggttcg
    17941 ctgcacgatt ttctgcgcga gcaaaccgga attgcgctgc gccgcagcca gacgcggatc
    18001 agcgcccgcc gcgcccaggc caaagagtgc cagcgccttg aaatcccgaa tatgtcgccg
    18061 ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc ggagtactcc
    18121 gtcagcctga cgcgcgccga catgattgaa ttcactatgg agcactgaat gcacgcagat
    18181 accgcgaccc gccagcactg gatgtccgta ctggcgcaca gccaaccggc tgaactggca
    18241 gcccgcctga acgcgctgaa catcaccgcc gactatgaag tgatccgcac cgccgaaacc
    18301 ggcctggtac agattcaggc gcgaatgggc ggcaccggcg aacgcttttt tgccggcgac
    18361 gccacgctga cccgcgccgc cgtacgcctg actgacggca ccctcggcta cagctgggtg
    18421 ctggggcgtg ataaacagca caccgaacgc tgcgcgctga ttgatgcgct gatgcagcaa
    18481 tctcgccact ttcaaaactt atcagaaacc cttattgccc cgctggacgc tgaccgtatg
    18541 gcacgcattg ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt ctttacgatg
    18601 gttcgcggag acaacgcatg accctggaaa ccgcttttat gcttcccgtg caggatgccc
    18661 agcacagttt tcgtcgcctg ttaaaggcca taagcgagcc gggcgtgatt gtcgccctgc
    18721 atcagctcaa gcgcggctgg cagccgctga atatcgccac caccagcgtg ctgctgacgc
    18781 tggctgataa cgacacgccg gtgtggcttg ccgcgccatt gagtaacgat atcgtcaatc
    18841 agagcctgcg ttttcatacc aacgcgccgc tggtcagcca gccggaacag gcgaccttcg
    18901 cggtgacgga tgacggaatt tccagcaaac agctcaacgc cctttccacc ggcaccgccg
    18961 ttgcgccgga agcgggcgcg acgctgattt tacaggtcgc cagcctgagc ggcgggcgca
    19021 tgttgcgtct caccggcgcg ggtattgccg aagaacgaat gatcgctccg cagctgccgg
    19081 agtgcattct gcacgaactc accgagcgcc cgcacccgtt cccgctcggc atcgacctga
    19141 tcctgacctg cggcgaacgc ctgctggcta ttccgcgaac cactcatgtg gaggtgtgct
    19201 gatgtacgtt gccgtgaaag ggggcgaaaa ggcgatcgac gccgcccacg ccctgcaaga
    19261 gagccgacgc cggggcgata ccgatttgcc cgaactgagc gtcgcccaaa ttgaacagca
    19321 gcttaatctc gcggtggatc gcgtgatgac cgaaggcggc gttgccgacc gcgaactggc
    19381 ggcgctggcg ctgaaacagg ccagcggcga taacgttgaa gcaattttcc tgctgcgcgc
    19441 ctaccgcacc acgctggcga aactggcggt aagcgagccg ctcgacacca ccgagatgcg
    19501 tctcgaacgg cgtatttcgg cggtttataa agacattccc ggcggccagc tgcttggccc
    19561 aacctacgac tacacccatc gcctgctcga tttcaccctg ctggcaaacg gtgaagcgcc
    19621 gacgctgacc accgccgaca gcgaacaaca gccgtcgccg cacgttttca gcctgctggc
    19681 gcgtcagggg ctggcgaagt ttgaagaaga tagcggcgca cagccggatg acatcacccg
    19741 cacgccgccg gtttacccct gctcacgctc ctcccgcctg caacaattaa tgcgcgggga
    19801 cgaaggctat ttgctggcgc tggcctactc cacccagcgt ggttacggac gcaatcaccc
    19861 gttcgcgggc gagatccgca gtggttacat cgacgtgtcg attgtgccgg aagagctggg
    19921 atttgcggta aacgtcggcg aactgctgat gaccgagtgt gaaatggtca acggttttat
    19981 cgacccgccg gatgagccgc cgcacttcac gcgcggctac gggctggtat tcggcatgag
    20041 cgagcgcaaa gcgatggcaa tggcgctggt tgaccgtgcg ttgcaagccc cggaatacgg
    20101 cgagcacgcg acaggcccgg cgcaggatga agagttcgtg ctggcgcatg ccgacaacgt
    20161 cgaagccgca ggctttgtct cgcacctcaa actcccccac tacgtcgatt tccaagccga
    20221 actggagcta ctcaaacgtc tgcaacagga gcagaaccat ggctaatctg agcggctaca
    20281 actttgccta cctcgacgag cagaccaaac gcatgatccg ccgcgccatc ttaaaagcgg
    20341 tggctatccc cggttatcag gtgccgtttg gcgggcgcga gatgccgatg ccctacggct
    20401 ggggaaccgg cggcattcag ctcaccgcca gcgtgattgg cgaaagcgac gtgttgaagg
    20461 tgattgacca gggcgcagac gacaccacta acgccgtgtc gattcgcaac ttcttcaagc
    20521 gcgtgaccgg ggtgaacacc actgaacgta cggacgatgc gacgcttatc cagacgcgcc
    20581 atcgcatccc cgaaacgccg ctgaccgaag atcagatagt tattttccag gtgccgatcc
    20641 ccgaaccgct gcgctttatc gagccgcgcg aaacggaaac ccgcactatg cacgcgctgg
    20701 aggagtacgg cgtgatgcag gtgaaactgt atgaagatat cgcccgcttc ggtcatatcg
    20761 ccaccaccta tgcttatccg gtgaaggtga acgggcgcta cgtgatggac ccgtcgccga
    20821 tcccgaaatt cgataacccg aaaatggaca tgatgcccgc cctgcagctg ttcggcgcag
    20881 ggcgcgagaa gcgcatctat gcggtgccgc cgtttacccg cgtggaaagt ctcgatttcg
    20941 acgatcaccc gttcaccgtt cagcagtggg atgagccatg cgccatctgc ggatcgaccc
    21001 acagctatct cgacgaagtg gtgctggatg acgccggaaa ccgcatgttt gtctgctccg
    21061 ataccgatta ttgccgccaa cagagcgagg caaaaaacca atgaatcaac cgttactttc
    21121 ggtcaataac ctgacccacc tttacgcgcc gggcaaaggc tttagcgatg tctcttttga
    21181 tttatggccg ggggaagtgc tgggcatcgt cggggaatcc ggctccggga agaccacgct
    21241 gctgaaatca atctccgcgc gcctgacgcc gcagcagggg gagattcgct acgagaatcg
    21301 ttcgttgtat gcgatgagcg aggccgaccg ccgtcgcctg ctgcgcaccg aatggggcgt
    21361 ggtgcatcag catccactcg acggcctgcg tcgccaggtc tcggcaggcg gcaatatcgg
    21421 cgagcggttg atggcgaccg gggcccgtca ttacggcgac atccgcgcca ccgcgcagaa
    21481 gtggctggaa gaggtggaga ttcccgccaa ccggatcgac gacctgccga ccaccttttc
    21541 cggcggcatg cagcaacgtt tgcagattgc ccgcaacctg gtgacgcatc cgaagctggt
    21601 gtttatggac gagccaaccg gtgggctgga tgtgtcggtg caggcacgcc tgctcgatct
    21661 actgcgcggc ctggtggtgg agctgaacct ggcggtggtg attgtcaccc atgatttagg
    21721 cgtcgcccgc ctgctagcgg accgtttgct ggtgatgaag caggggcaag tagtggagag
    21781 tgggttaacc gaccgcgtgc tcgacgaccc gcaccatccg tatacccagc tgctggtgtc
    21841 atcggttttg cagaattgag ccggtgccgg atgcggcgta aacgccttat ctggcctaca
    21901 aatgcgctcc ccgtaggtcg gataagacgc atcagcgtcg catccgacac ccgaaccacg
    21961 aggcgaaaaa tgattaacgt acaaaatgtc agtaaaacct tcatcctgca ccagcaaaac
    22021 ggcgtgcgcc tgcccgtcct caaccgcgcc tcgctcaccg tcaacgcggg cgaatgcgtg
    22081 gtgctccacg gccactccgg cagcggcaaa tcaaccctgc tgcgctcgct gtacgccaac
    22141 tatctgcccg atgaagggca aatccagatc aaacacggtg acgagtgggt agacctggtc
    22201 accgcgccag cgcgcaaagt ggtggagatc cgcaaaacca ccgtcggctg ggtgagccag
    22261 tttctgcgcg tcatcccgcg tatttcagca ctggaagtgg tgatgcagcc gctgctcgat
    22321 accggcgtac cgcgtgaagc ctgcgccgct aaagccgcgc gtctgctcac ccgcctgaac
    22381 gtgccggaac gcctgtggca cctggcgcca tcaaccttct ccggcggcga acagcagcgc
    22441 gtcaacatcg cccgcggctt tatcgtcgac tacccgattc tgctgctcga cgaacctacc
    22501 gcctcgctgg acgccaaaaa cagcgccgcg gtggtggaac tgatccgcga agccaaagct
    22561 cgtggcgcag ccattgtcgg catcttccat gacgaagccg tacgtaatga cgttgccgac
    22621 cgcctgcacc caatggaagc ctcttcatga ttatcaataa cgtcaaattg gtgctggaaa
    22681 acgaagtggt acacggttca ctggaggtgc aggacggcga aatccgcgcc tttgccgaaa
    22741 gccagagccg cctgccggag gcgatggacg gcgaaggcgg ctggctgctg ccggggctga
    22801 ttgagctgca taccgataat ctggataaat tcttcacccc gcgcccgaaa gtcgactggc
    22861 ccgcccattc ggcgatgagc agccacgacg cgctgatggt gacaagcggc atcaccaccg
    22921 tactggacgc cgtggcgatt ggtgatgtgc gcgacggcgg cgatcggttg gagaatctgg
    22981 agaagatgat caacgccatc gaagagacgc agaaacgcgg cgtcaaccgc gccgagcacc
    23041 gtctgctcct gcgctgcgaa ctgccgcatc acaccacgct gccgctgttt gaaaaactgg
    23101 tgcagcgcga gccggtgacg ctggtgtcgc tgatggacca ctcgccgggc cagcgccagt
    23161 tcgccaaccg cgagaagtat cgcgaatatt atcagggcaa atactcgctc accgatgcgc
    23221 agatgcagca gtacgaagaa gagcaactgg cgcttgccgc acgctggtcg cagccgaatc
    23281 gcgaatccat cgccgccctg tgccgcgcgc gacaaattgc gctcgctagc catgatgacg
    23341 ccacccacgc ccacgttgcc gaatcccacc tgcttggcag cgtgatcgcc gaatttccca
    23401 ccacgtttga agcggcggaa gcctcgcgca agcatggcat gaacgtactg atgggcgcgc
    23461 cgaatatcgt gcgcagcggt tcgcactccg gcaacgtggc ggccagtgaa ctggcgcagc
    23521 ttggcctgct ggatatcctc tcttccgact actaccccgc cagcctgctg gatgcggcat
    23581 ttcgcgtcgc cgatgaccag agcaaccgct ttacgctgcc gcaggcggtg aagttagtga
    23641 cccaaaatcc ggcgcaggcg cttaatcttc aggatcgcgg ggtgattggc gagggcaaac
    23701 gcgcggacct ggtgctggca catcgcaagg acaatcatat tcatatcgac cacgtctggc
    23761 gtcagggtaa aagggtgttc tgatgatggg aaaactgatt tggttaatgg ggccgtccgg
    23821 ctccgggaaa gacagcctgc tggcggaaat ccgcctgcgg gaacaaactc agttactggt
    23881 ggcgcatcgc tacattacgc gcgacgccag cgccggaagt gaaaaccata tcgccctgag
    23941 cgagcaggag ttttttaccc gcgcggggca aaatctgttg gccttaagct ggcacgctaa
    24001 cagtctgtat tatggcgtcg gcgttgagat tgatctctgg ctgcacgccg gattcgacgt
    24061 gctggtcaac ggctcacgcg cccatctgcc gcaggcgctg gcgcgctatc aatcggcgct
    24121 gctgcccatt tgcttacagg tttcgccgga gatcctgcgc cagcgcctcg aaaaccgtgg
    24181 tcgtgaaaat gccagtgaaa ttaacgcccg actagcacgc gctgcccgct atactcctca
    24241 ggattgtctt acgctcaata atgacggcag cctgcgccag tcggtcgaca agctgctgac
    24301 gctgattcat cagaaggaga aacaccatgc ctgcttgtga gcttcgcccg gctacgcagt
    24361 acgacaccga cgcagtttac gcgctgattt gtgagctaaa acaggcggag tttgaccacc
    24421 atgcgtttcg cgtgggcttt aacgccaacc tgcgcgaccc aaacatgcgc taccatctgg
    24481 cgctgctgga tggcgaagtt gtcagcatga tcggcctgca tttgcagttt catctgcatc
    24541 atgtcaactg gatcggcgaa attcaggaac tggtggtgat gccgcaggcg cgcggtctga
    24601 acgtcggcag taaattactg gcatgggcag aagaagaagc ccgccaggcc ggggccgaaa
    24661 tgaccgcgct ttcgaccaac gtgaaacgcc acgacgcgca ccgtttctat ctgcgagaag
    24721 gctacgagca gagtcacttc cgcttcacca aggtgctgta acatgagcct gaccctcacg
    24781 ctcaccggca ctggcggcgc acagggcgtt ccggcatggg gctgcgagtg cgcggcctgc
    24841 gaccgagcgc agcgctcacc gcagtatcgc cgccagccat gcagcggcgt agtgaagttt
    24901 aacgacgcaa tcaccctgat cgacgccggg ctgcacgatc tcgccgatcg ctggtcgccc
    24961 ggatcgttcc agcagttttt gctgacgcat tatcatatgg atcacgccca ggggctgttt
    25021 ccgctgcgct ggggcgtggg cgatccgatc ccgatctacg gcccgccgga tgaccagggt
    25081 tgcgacgatc tgtttaaaca tccgggcctg cttgatttca gccacacagt ggaaccgttt
    25141 gtggtgtttg atttgtaggg gttgcaggtc acgccgctgc cgcttaacca ctcaaaactg
    25201 accttcggtt atctgctgga aacggcacat agccgggtgg cgtggctatc tgacaccgct
    25261 ggcctgccgg aaaaaacgct gaaattttta cgcaataatc agccacaggt aatggtgatt
    25321 gattgcagcc acccgccgcg cgcggatgcg ccgcgtaatc actgtgattt aaataccgtg
    25381 cttgcgctga atcaggttat ccgctcgcca cgggtgattc tgacccatat cagccaccag
    25441 tttgatgcgt ggctgataga aaacgcactg ccgtcggggt ttgaggcggg gtttgatggg
    25501 atggagattg gggtggcgtg atgagaggga atgtgcgcgc tggtcccctc accctaaccc
    25561 tctccccaga ggggcgaggg gagcgattgt gctcgatatt gaatattgcg ctggttttct
    25621 ccctctcccc attggggtga ggggcgatgc ctgctccata cccaacctca tcgcccatac
    25681 tcatcttcca ttctccgctc ttcatcttct agctgccgac gctcctgatc aagctggcgc
    25741 tggcgatcgt ccagctgcct gcggcgatct tcaaactggc agcggcggtc gtcatattgt
    25801 ctgcgccgat cgtcgctcac ttcacgctgc cagccgtcgt cgcgcgaatc ttcatagtct
    25861 cgcccacggt cagagttata agcgtcatta atcgcctgct gaatattacc aatagtatcg
    25921 tcgataatat cggcctgggc cggaacggtg gacagcgtga gcagggtgaa aagaaaaagc
    25981 gtggagtaag gtttcataag ccagtctctg cgatggactg gcttaacgtt agttgagtgt
    26041 gaatgaccgg agtagcggag taatctcaaa ttcggtaagg cgcagactct tccattagct
    26101 ggttagctcg ctgtttattt tccgccacca atacaatgcc atgacatttt tgatccggtg
    26161 aaatactcca gatacgctct tccggaatgc agccgccatc gtagatcact ttcgtgccat
    26221 cgtagggcca aaatgcccct tgttcagtgt ctaatacaaa cacggaaatc tgatcggact
    26281 cgcgcgcgat cttctgccag agcatcttcc cgccatcata ttgatcaaaa tcgctgatta
    26341 gcgtgatacc gcattgcagg attagcgctt catacagccg agttgccaga cccttatcgc
    26401 gctcacctgg agagacacaa ataccttcca cctgtaccag atccgagtct tcccaccagc
    26461 gttcaacggc aatgatatcg ctgccgaact cttttttacg ctgcagatcg agagcggcaa
    26521 gtacccgatg acgcggcacg cctgcaaaaa cttcaatgtc cgcttcaatg atggccactc
    26581 ttggcgtctg gttgtcataa attacctgta acagacgtcc agaaagctcg atctcttcca
    26641 ccacgcgaaa agaacgagca accgtcatag ggctataggt ggttggcatc acgatcatat
    26701 gtaaatcact tcaattcaga acagaattgc tcacaatagc agcacgtttg cttcgagcct
    26761 cttgataaat tctgaaaatc atccctcaat ttgccccagc acctgcgcca gctgcgcgcg
    26821 ggtaaagggt ttgcgcagca gcgccacgtc cggcagcgcg gggttatggc tggggcgtaa
    26881 atcctgaccg ctgatgagta gcagcgtcag gtgcgggtaa agtttgcggg ccgcattgac
    26941 cacttctgcg ccgctcatgc cgccagcagg tccagcgcct gttcgccgct gctcgcttcc
    27001 agtgtcaaat agcccagcag gtgcaactgc tcgcagatgg tctggcgcac cgcggcttca
    27061 tcttccagca ctaacaccag tttatcgcca ttaacagctg tctgttctac cgccgctggc
    27121 gataggtttt tcgccgccgt cagcgcgcgg ggaagctgta atctgaccgt tgtcccctgc
    27181 cccggcgcgc tttcaatttc cacgcgcccg ccggactggc gcacgaaacc atagaccatc
    27241 gacaagccca gcccgctgcc gctccccgtc tgtttggtgg taaagaacgg ctcgaacacc
    27301 tggcttttca ccgcctgcga cataccgctg ccatgatcga tgacctccag tgccaccatg
    27361 tcctgacgct gcccactgct gcgggtaaca cgctgattcc aggtacgaat tttgataatt
    27421 cccgtctgcc cttccatcgc gtcgcgggcg ttcatcacca ggttgatgat ggcgttttcc
    27481 agttggctga cgtcaatcca cgcggaccac gccggagact gcgcttcaat ctcaatcgtc
    27541 agcgtcgccg gtaaggagtg gcgcattaat tcgcttaact cttccagcag tgtttttagt
    27601 tcaacggctt gcggatgtaa ggactgcttg cgggaaaatg ccaacaatcg ctgggtcagt
    27661 agcgcgccac gttcggcggc ttgctgcgcc cgctgtattc gcggcgcgtc gggtgagttc
    27721 gtgttcacca gttccaggct accaataatt accgccagca gattattaaa atcatgggcc
    27781 agcccgcccg tcagttgcct gaccgctttt aacttctggc tgtgcaatag cgcctcttcc
    27841 agcccctgcc gctcggtttg ggcaatctcc atctgcgtgg ttttctcttt caacagccgt
    27901 gacgttcgtt caagtgatgc ggtattgcgg gcaaaaacgt taaaggcgcg cgccagttca
    27961 cccaactcat cgcgccgctg taacgccgga aaagtgacgt ccgcttcgcc gtgcgccagg
    28021 cggctcatcg cgcgggaaat cgccgtcagg ttagagccga gattgcgata gatataccaa
    28081 cctgcaaacg cggtgatcac caccgctaac agggcgaaaa tcatgatcag cctctcgccg
    28141 gagcgcaaat cctggtaact ttgctccacc cgctgaccag agagcgtcgc cacctgttcg
    28201 gcgtaacggt tgatgtcgcc attaaggatc gccaccagcg ctttgatgtg aaacatatac
    28261 cagctgattg ccagatcgct ttgctccaga gccgccgaaa gtggcgcgag tttacgcagt
    28321 tcacgattaa aatcagcgag tagcgtatta accaatggcg tatcgctgtg agttggcaag
    28381 gcagacatca ctccgaccag ttgatgaata atcgcctgcg gcgcgggggt ggcaatggcg
    28441 gcaacaatca gtcggttcat ctcgttaagc aaaatattat cttgcgcgtg agtgactttt
    28501 tgcagatgcc gcagataact taagttttga tacagcgaac ttaacagcgc gttacgctcc
    28561 agatgccgcc tttgcccgcg ttccagcatt ccgccgacgc tggtttgcag ttcgttgcta
    28621 cgcgtgatga tgcgcgtcac cagcccggct tcctgctgcg ctaaaggcgc gttcgccagt
    28681 tgtgcaagcg agctttttaa tgcccgctgt gtctcctgca accgctcggc ttcgccttta
    28741 tattccagtg ctcccaccac ctgcgacaat cgcactgccg ccgttgccac gtttgccgtg
    28801 tcgcgcgcca gattcaggct gccggtcata tcatctacag tttgctgctg caccagctct
    28861 tgcatctggc tggcatgacg aaagcccatt accgccacgc cgctgaccat caacgtcacc
    28921 gccaccacca gcaaattcaa aagcagcaat cgcccgcgcg cgctggcaaa aaagtgacgt
    28981 ccggggtgcg ccatatcacg ctccgtgaag aaaaatcgca atatgacaaa tatgaacggc
    29041 ttctgacatt ttgcatttac tttcctgtga tggagtgctg atatcgacaa cctcgcagga
    29101 gtccaccatg aacagcacgc cggttcttga gatgcgcaat attgccaaag ccttcggcaa
    29161 attttatgcg ctcaaagggg tggatttgac ggtctaccct ggcgagatcc atgccctgat
    29221 gggggaaaac ggcgcgggaa aaagcacgct gatgaaggtg ctggcggggg cgtataccgc
    29281 caccagcggc gagatcctga tagacggcaa gccctttcac attcgcacgc caaaagatgc
    29341 cttaagcgcc ggtattacgc tgatttatca ggagatgcag ctggcaccca atttgacggt
    29401 ggcagaaaat attttcctcg gcagcgagct ttcccacggc gggctggtgc agcgaaaaga
    29461 gatgctggtg caggcgcaaa gagtgatcga ccgcctcggc gcgcagttta acgccagcga
    29521 taaggtcatg acgctgacca ttgccgaaca acagcaggtc gaaatcgccc gcgcgctaca
    29581 tcgcaacagc cgcattctgg tgatggacga acccaccgct gccctctcct cccgtgaaac
    29641 tcaccgtctt tttgaactga ttatgcggct gcgcgatgaa gggatggcga ttatctacat
    29701 cagccaccgt atggcggaag tgtatgagct ttccgatcgc gtcagcgtgc tgcgcgacgg
    29761 gcaatacgtt ggcagcctga cccgcgataa cctcaatgcc ggggagctgg tgcggatgat
    29821 ggtcggcagg ccactgagcg atctgttcaa taaagagcgc gatatcccgc tcggtaaagc
    29881 ccgcctgaat gttcaccacc tgaccgacgg cggcaaagtc cagtcgagct gcctgcaggt
    29941 gctttccggc gaaattgttg gcctcgccgg gctggtggga gccggacgtt ccgaactggc
    30001 gcagttaatt ttcggcgtgc ggaaagcgac aggcggaatg attgaagtcg atggcgaacc
    30061 ggtggtgatc cactccccgc gcgaagccat cgatcttggc attggttttc tcaccgaaaa
    30121 ccgcaaagaa caaggcttat tccttgaaat ggcggcagtc gaaaacatca ctatggcaac
    30181 cctggagcgc gatgccagct ggggaatgct caatcgcaaa aaagcgcaaa ccatttccga
    30241 tgacgccatt aagttgctca acattcgcgt gcctcatgcc caggtacgcg cgggcgggct
    30301 ttccggcggc aatcagcaaa aactgttgat ctcccgctgg gtggcgattg gcccgcgcat
    30361 tttactgctc gatgaaccca cccgcggcgt ggacgttggc gcaaaaagtg agatctaccg
    30421 gatcatgaac gagatggcgt gcaagggcgt ggcgatcctg atgatctcca gcgaactgcc
    30481 ggagatagtg ggaatgagcg atcgcgtcca tgtgatgcgc gaaggcagca ttgccggtga
    30541 gctaaatggc aaaaacatca cccaggaaaa cattatgacc ttagcgactg gcgtcaacga
    30601 cgcccattcc caggcggtga cctcatgacg acctctgcaa aaacctctgc caaaaaaatg
    30661 ctgatgagcg atctgatgca aacggtcggc attttgccca ttttgatcct gatcgtagcg
    30721 gtatttggct ttatcgcgcc aaactttttt accgaaagca acctgctaaa tattacccgc
    30781 caggcgtcga tcaacattgt gctggcggcg gggatgacct tcatcatttt gaccggtggg
    30841 atcgatcttt ctgtaggttc aatcctcggc accacggcgg tggcggcaat ggttgtctcc
    30901 cttagcccgg agttcgccat gttgtctatc ccggcggcgc tgatgctcgg cctgctgctg
    30961 gggctgttca acggcgcgct ggtggccttt gccggattgc cgccgtttat cgtcacgctt
    31021 ggcacctata cggcgctgcg cggggcggcg tatctgctgg ctgatggcac cacggttatc
    31081 aactccaaca ttaatttcga gtggataggc aataactatc tcgggccggt cccgtggctg
    31141 gtggtgattg cgctggcggt gatcgtgctg tgctggttta ttctgcgtcg caccacgctt
    31201 ggcgttcata tctacgccgt cggtggcaat atgcaggcgg cgcgtcttac cggcattaag
    31261 gtctggctgg tgctgctgtt tgtttatggc atgagcggcc tgctttcagg gcttggcggg
    31321 attatgagca cctcgcggct gtacagcgcc aacggcaact taggtatggg ttacgaactg
    31381 gatgccattg cggcggtgat cctcggcggc accagttttg tcggcggcat cggcaccatc
    31441 accggcacgc tggtgggggc gctaattatc gccacgctca acaatggcat gaccttaatg
    31501 ggcgtctctt acttctggca gctggtgatc aaaggggcgg tgatcatcat tgcggtgctt
    31561 atcgacaaat accgtacccg acatcatcaa agtgcataac aacaataccc tacacatgag
    31621 gaatacagca tgagattgaa gcccttagtg accgcgctct gtgcaggcgc gctgctggcc
    31681 gcagcaccgt tcgcccaggc gaaagatctg aaatcgatag gcgtaacggt aggtgacctg
    31741 gcgaacccgt tcttcgtaca gatcaccaaa ggcgcggagc tggaagcacg caaactggcg
    31801 ggcgataacg tcaaagtgac gctggtttcc agcggctacg atctgggtca gcaggtctcg
    31861 cagatagaca actttatcgc cgctaacgtc gatatgatca tccttaacgc tgccgattca
    31921 aaagggatcg gcccggcggt gaaacgggca aaagacgccg ggatcgtggt ggtggcggtt
    31981 gacgtggcgg cagaaggtgc cgatgccacc atcacttccg ataacaccca agcaggcgaa
    32041 atggcctgta aatacatcac cgatcgcctg aaaggcaaag gcaacgtggt gatcatcaac
    32101 ggaccgccgg tgtccgccgt gcaaaaccgc gtcgaaggct gccagaccga atttaaacgc
    32161 catcctgaca tcaaggtgct ctctgacaac aagaacgcca aaggcagtcg tgagggcggg
    32221 ctggaggtga tgaccgggct gctggcggca aatccgaaga tcgacggcgt atttgcgatt
    32281 aacgatccga cagcgatcgg tgccgatctg gcagcaaaac aggcgcaacg taacgagttc
    32341 tttattgtcg gcgtggatgg aagcccggac gccgaagagg cgctcaagcg tgagaactcg
    32401 ttgtttgtcg ctactccggc gcaagatcca caggtgatgg cagcgaaagc ggttgagatc
    32461 agttatgaca ttttgcaggg caaacctgcg ccgaaagagc cggtgctgat cccggtgacg
    32521 atgatcgata aaaacaacgt cggcaattac aaaggctgga cggtgaaata agtccacacg
    32581 ccgggcggcg atgctgcccg gccaccgcta aggagaacgc tttcgatgaa acgctccgct
    32641 ataaacaaca ttctcggcca tacacggcaa ttcttttcgc aacatgacgt acatctgccg
    32701 ccctttgcca gtttctctcc tgctcaatgg cagcagctcg acaccgccgc gtgggaggtg
    32761 gtttttgatc tcaaactcgg ctgggatgtt accgcttttg gtggtaacaa ctttgcagca
    32821 caggggctga cgctgtttac cctgcgtaac ggctcagcaa aaggaatgcc gtatgtgaaa
    32881 tgttacgccg agaagatcat gcatgtgcgc gatgcgcagg tcacgccaat gcattttcac
    32941 tggcgtaagc gtgaggacat catcaatcgc ggcggcggca atttgattgt tgaactgtgg
    33001 aatgccgata gcaacgagca aacagttgat accgacgtta cggtggtgat cgacggctgc
    33061 cgccagactc atgtcgcagg cagtcagtta cgcctgtccc ccggcgaaag tatctgcctg
    33121 ccgcccggcc tgtatcacag cttttgggcg gaaaccggtt ttggcgatgt tctggttggc
    33181 gaagtctctt ctgtcaatga cgacgaccac gataaccact ttttacaacc gctggaccgc
    33241 tacaacctga ttgacgaaga cgaaccggcc cagctggtgt tgtgtaacga ataccgccag
    33301 ttcctctgaa taaggataac gttatgccgt tgatttctct cgctgaaggg ctggcacatg
    33361 cccgcgaaca tcactatgcg ttaggtgcgt ttaatgtgct ggactcacac tttttacgcg
    33421 ccttgtttgc tgctgccaga caagaacgtt cgccctttat tatcaacatt gccgaagtac
    33481 attttaaata tatctcgctg gattccctgg tcgagacagt gaaatttgaa gcggcgcgtc
    33541 aagcgattcc ggtggtatta aatctcgatc acgggctgca ttttgaatcg gtggcgcaag
    33601 cgttgcggct gggattcagc tcggtaatgt ttgatggctc cacgctgggg tatgaagaga
    33661 atatccgcca gacgcgggaa gtggtgaaaa tgtgccatgc cgttggcgta tcggtagaag
    33721 ctgaactggg cgcggtgggt ggtgacgaag gcggtgcgct ttacggtcat gccgatgaat
    33781 ctctgtttac cgatccgcag ctggcgcgtg attttgtcga ccgtaccggc attgacgcgc
    33841 tggccgtggc gattggcaat gcgcacggca aatatcgcgg cgaaccgaaa ctcgatttcc
    33901 cgcgcctcga cgctatccgc cagcaggcgg ctatcccgct ggtattacac ggcggttcag
    33961 ggattagaga tgccgatttc cgccgcgcca tcgaactggg tatccacaaa atcaatttct
    34021 acaccggcat gtcacaagcg gcgctggctg ccgttgaggc ccgaatggcg caccgtcagc
    34081 cgatgtatga cgagtttgcc gagttactac tagcagtgga agccgccatc agcgatacgg
    34141 ttgcacaaca gatgcgtatt ttcggcagtg cggggcaggc atgatggaac gtaaagggat
    34201 tatcgccgca ggcaatatgc tggttgacca cgtccaccag attatccagt ggccagagcg
    34261 cggctggctg gcggagatta cccacagcga acgctccacc ggcggcgcgc cgcttaacgt
    34321 cctgctgaca ctggcgaaaa tgcgcaccgg gttgctgtta caggcggtcg ggctaatcgg
    34381 tcaggacagc gacggcgatt acattatggc gatgcttgag caatatcacg tcaatcgtca
    34441 gcatgtgcag cgcactacct ttgccccgac gtcaatgtcg caggtaatga ctgatccaag
    34501 cgggcagcgg acgtttttcc attcgccagg tgcaaacaga cttttggatc ttccggcgtt
    34561 cgatcagctc gataacacca tgaagatttt ccatctcggc tatttactgt tgctcgacag
    34621 cctggatatg ccggacgatg tttacggcac ccgtagcgca cgcctgctgg cgcaaatgcg
    34681 tgatactggc tttgaaacct cactcgacct ggtctcccgc aagggcgatc cgcgttatca
    34741 gccgctggtg ttgcccgcgt tgcgccatct ggattacctg gtaattaacg aactggaggc
    34801 cggggagttt agcgggctgg aaattcggtt acccaacggc gaaccgcata tcgcgcatat
    34861 tgctgccgcc gcgcgtgaac tgttagatgc gggcgtgcgc cagcgtgtgg tgatccactg
    34921 cccggaaggg gcgtggggag agaccccgga acagtgcggc gtgtggatcc cttcgcaaaa
    34981 gttagaacaa cgggaaatta tcggcagcgt cggtgcgggc gatgtatttt gtgccggatt
    35041 tttatatggc tgccatgaac gctggacgct taccgagagc atcaaactgg cacacgcctg
    35101 cgcgcgagca agcctgttat gcgccaatgc catcgacggg gcgaaaacgc tggaagagct
    35161 gcaaacagaa atgtcggatt aatgcacgtc ggcagcgaag acatacccca gaccgcgcag
    35221 agttttaatc aacatcggct gatgcgggtt gagttcgatt ttgcgtcgta agcgcatgat
    35281 taatacgtcg atggtgcgat caaacacctc cacgctgtcg ctatgagtga acgccagcag
    35341 ctgttcgcgg cttaataccc gacgggcatt ttgagccagg gccagtaaca agccatattc
    35401 gccctgagtc agctcgacat gttgttgtag cggactatgc agttggcagc gaatggtatc
    35461 gagataccag ccattgaatg acagcccggc gtttttctcc tcattaaccg ccagcgcgcc
    35521 aacccgtcgc aacacgcttt cagtcgtgcg accaccaccc gtggggtgaa cggcttgcag
    35581 atgtaatcat ccgcgcccat ttccagcccg accaccacat cagactcact ccccattccg
    35641 gtcaacatca ccacaggcaa tgccggacgc agtttctgga tttgctgcaa taccctcagg
    35701 ccgttggtat cgggcagcat catatccagc accaccagcg caatgtcagg ctcgccctca
    35761 atacgcgata tggcctcctg tccggtatgg ctgactgaaa ccgtaaagac atgttcactt
    35821 aacacgtcct gcaacagtgc gcagatagcc gtgtcatcat ccaccaccag cacgacgggt
    35881 ttcatggtta cactccgcat cattcatcta attaatcatt gtgcgtactt cttgtcgaag
    35941 tgaaagcgga aagtaatata aataataaaa atataagtca ggtcacatta ttgat
//
LOCUS       DADMFL010000029        35845 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.029, whole genome shotgun sequence.
ACCESSION   DADMFL010000029 DADMFL010000000
VERSION     DADMFL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.029"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..218)
                     /locus_tag="JNZ27_001444"
     CDS             complement(<1..218)
                     /locus_tag="JNZ27_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8663830.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(399..1874)
                     /locus_tag="JNZ27_001445"
     CDS             complement(399..1874)
                     /locus_tag="JNZ27_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8663831.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3304)
                     /locus_tag="JNZ27_001446"
     CDS             complement(2021..3304)
                     /locus_tag="JNZ27_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY8663832.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSSESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIEHVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(3417..5351)
                     /gene="yeaG"
                     /locus_tag="JNZ27_001447"
     CDS             complement(3417..5351)
                     /gene="yeaG"
                     /locus_tag="JNZ27_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY8663833.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5787..6533
                     /locus_tag="JNZ27_001448"
     CDS             5787..6533
                     /locus_tag="JNZ27_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY8663834.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6623..7384
                     /locus_tag="JNZ27_001449"
     CDS             6623..7384
                     /locus_tag="JNZ27_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY8663835.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7435..8319)
                     /locus_tag="JNZ27_001450"
     CDS             complement(7435..8319)
                     /locus_tag="JNZ27_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY8663836.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8403..9398)
                     /gene="gapA"
                     /locus_tag="JNZ27_001451"
     CDS             complement(8403..9398)
                     /gene="gapA"
                     /locus_tag="JNZ27_001451"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8663837.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9740..10153
                     /gene="msrB"
                     /locus_tag="JNZ27_001452"
     CDS             9740..10153
                     /gene="msrB"
                     /locus_tag="JNZ27_001452"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY8663838.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10195..10467
                     /locus_tag="JNZ27_001453"
     CDS             10195..10467
                     /locus_tag="JNZ27_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY8663839.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10837..11913
                     /locus_tag="JNZ27_001454"
     CDS             10837..11913
                     /locus_tag="JNZ27_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8663840.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11940..13319
                     /locus_tag="JNZ27_001455"
     CDS             11940..13319
                     /locus_tag="JNZ27_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663841.1"
                     /translation="MEQITKPHCGVRLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESCGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13336..14382
                     /locus_tag="JNZ27_001456"
                     /pseudo
     CDS             13336..14382
                     /locus_tag="JNZ27_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14403..15239
                     /locus_tag="JNZ27_001457"
     CDS             14403..15239
                     /locus_tag="JNZ27_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY8663842.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15244..16192
                     /locus_tag="JNZ27_001458"
                     /pseudo
     CDS             15244..16192
                     /locus_tag="JNZ27_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16202..17182
                     /gene="ydjG"
                     /locus_tag="JNZ27_001459"
     CDS             16202..17182
                     /gene="ydjG"
                     /locus_tag="JNZ27_001459"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY8663843.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17319..18077
                     /locus_tag="JNZ27_001460"
     CDS             17319..18077
                     /locus_tag="JNZ27_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8663844.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18195..19553
                     /locus_tag="JNZ27_001461"
     CDS             18195..19553
                     /locus_tag="JNZ27_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663845.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTPALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="JNZ27_001462"
     CDS             complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="JNZ27_001462"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY8663846.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="JNZ27_001463"
     CDS             complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="JNZ27_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY8663847.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="JNZ27_001464"
     CDS             complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="JNZ27_001464"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY8663848.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23498..24049
                     /locus_tag="JNZ27_001465"
     CDS             23498..24049
                     /locus_tag="JNZ27_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY8663849.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24166..25209
                     /gene="selD"
                     /locus_tag="JNZ27_001466"
     CDS             24166..25209
                     /gene="selD"
                     /locus_tag="JNZ27_001466"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY8663850.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25214..27175
                     /locus_tag="JNZ27_001467"
     CDS             25214..27175
                     /locus_tag="JNZ27_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY8663851.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27302..28342
                     /locus_tag="JNZ27_001468"
     CDS             27302..28342
                     /locus_tag="JNZ27_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8663852.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="JNZ27_001469"
     CDS             complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="JNZ27_001469"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY8663853.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30038..30310
                     /locus_tag="JNZ27_001470"
     CDS             30038..30310
                     /locus_tag="JNZ27_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY8663854.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="JNZ27_001471"
     CDS             complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="JNZ27_001471"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY8663855.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30770..31453
                     /locus_tag="JNZ27_001472"
     CDS             30770..31453
                     /locus_tag="JNZ27_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY8663856.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGY
                     LTLKSLQRQ"
     gene            complement(31462..32769)
                     /locus_tag="JNZ27_001473"
                     /pseudo
     CDS             complement(31462..32769)
                     /locus_tag="JNZ27_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(32836..33489)
                     /locus_tag="JNZ27_001474"
     CDS             complement(32836..33489)
                     /locus_tag="JNZ27_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8663857.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33489..35023)
                     /locus_tag="JNZ27_001475"
                     /pseudo
     CDS             complement(33489..35023)
                     /locus_tag="JNZ27_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34996..>35845)
                     /locus_tag="JNZ27_001476"
     CDS             complement(34996..>35845)
                     /locus_tag="JNZ27_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8663858.1"
                     /translation="LKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGG
                     AQLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDP
                     AALKEAPDDATFARVTAPLWQYLDALYPYLWREGKDFPPSPARMDALLKAGTLRLSLT
                     FNPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPD
                     AQLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWL
                     RRYGTH"
BASE COUNT         9083 a         8477 c         9180 g         9105 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttagggataa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gcgtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggctg gcctggccct
     3001 gaccactgcc ggaacctccg ccgccaccct ggggacgttc aattcggtcg ttctgaacga
     3061 aatggtcatt gcccggatgc acgcggtggc gcagaccgcc acgcccctga tgaaacattg
     3121 gttcgctaat atcttccgtg ggaatggata cggattcgct gctgtcgacg tcagtcaccg
     3181 aacgcttatt aatggcctcg gagatcgact gtttaatttg cgctttataa cggcgtaaaa
     3241 aacgctggcg attcaccatg cttttgtttt tgccgttcag acgccggtca ataaaccagg
     3301 tcatatgccc cccgtactac atttgccaac tgtatgctac gcctgtgttg ccgcttctca
     3361 ggcccacggt tcgctatcgt tgtaggcccg gtagcatgtg taccgggcca gggttgttaa
     3421 gacgatttac gtacgcgcaa ataccattcg cacagtaaac gcacctgttt acgggtgtag
     3481 cctttttcca tcatacggtc gacaaagtcg tcgtgtttct tttgctcgtc ggttgacgtt
     3541 ttggcgttaa acgagataac cggcaacagc tcctcggtat tggagaacat tttcttctcg
     3601 ataaccgtgc gcagtttttc atagctggtc cagttcggat tgcgtccgct gttattcgct
     3661 ctggcgcgca gtacgaagtt aacaatctcg ttgcggaaat cttttggatt actgatcccc
     3721 gccggtttct cgattttctc cagctcggcg ttaagagact cgcggtcaaa cagctgcccg
     3781 gtatccggat cgcgatactc ctgatcctga atccagaaat ccgcgtaggt aacataacgg
     3841 tcgaaaatgt tttgcccata ttcggaatag gattcaaggt aggccgtctg gatctctttg
     3901 ccgataaact cggcatattt cgggatcaga taacctttca ggaactccag atagcgttct
     3961 gcctgctctt gcgggaactg ctcgcgctcg atctgctgtt ccaggacgta gaacagatgg
     4021 accgggtttg ccgccacttc tacatgatcg aagttgaaca cgcgggagag gatcttaaac
     4081 gcaaaacgcg tcgacagacc gttcatccct tcatcgacac cggcgtagtc acgatattcc
     4141 tgatacgact tggctttcgg atcggtgtct ttcagacttt cgccatcata aacccgcatc
     4201 tttgaataaa tgctggagtt ttctggctct ttcaggcgcg aaagaatgga aaaacgtgac
     4261 agtgtttcga gcgtgccagg ggcgcatggg gcgtgagtca attcactgtg attaagcaat
     4321 ttctcgtaga ttttgatctc ttcggaaatg cgcaagcaat acggcacctt cacgatgtaa
     4381 acacggtcga ggaaggcttc gttgtttttg ttattacgga aagtgaccca ttcggactcg
     4441 ttcgagtggg cgagaataat cccgttgaac ggcagggcgg agataccttc cgtcccgttg
     4501 tagttatctt cctgagtggc ggttaacaag ggatgcagca cttttatcgg tgctttaaac
     4561 atctcaacga attccatgat cccctgattg gcgcggcaca gcgcaccgga atagccgtag
     4621 gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa tatcgacttt cccaaccagc
     4681 gcggagatgt cctggttgtt ctcatcaccg ggttccgttt tggcgatagc aatttgttgc
     4741 agaattgacg gccagacctt cactacccgg aacttagtga tatcgccacc aaattcatgc
     4801 aggcgttttg ccgcccatgg cgacatgatg gtgccgagat aacggcgagg aatgccatac
     4861 tctttttcca gaatctgagc atcttcctgc ggattgaaaa gacagaacgg atgatcgttg
     4921 accgggctac gctcaccgtt ggcgctcaat acataaatcg gtacgagctg cattaatgat
     4981 ttcagtcgct cagcaagcga tgatttaccc ccacccacag gccccagcag atacaggatt
     5041 tgtttcttct cttccagccc ctgagccgcg tgtttcagat aagagacaat ctgttcaatc
     5101 gcgtcttcca tgccgtaaaa ctcttcaaat gccggataac gtgcaatgac ccggttagaa
     5161 aagagtcgag aaagtctggg ttcctgggct gtatcgacca tgacaggctc accgatagcc
     5221 atcaatagcc gctcagccgc gttggcataa gcactgcgat cttgccgaca agtggtaaga
     5281 aactcctgca gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg atagtgatcg
     5341 aatatattca tggtatgccg tcctttcgtt ttttagcaca ggttaagagc cgttcatatg
     5401 aatagtagag gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa acacggcaac
     5461 tctcatgcca ttttatcgac gatgagaaca acgtgcccgg tgatacacct tcaaaattaa
     5521 gcgtagatgg caaatggaaa acttgcctgc acggctttct taatttcaat ggcatatcaa
     5581 caactcatcc ataaattttg cataattaat gtaaagacca ggctcgccag taacgctaaa
     5641 tccatttggc tgtaagcgcg gtgtcatccg cggcaggaaa attaaacagt tactttaaaa
     5701 aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg gtaaactttg tcgcccgcta
     5761 aacatttgtt taaggaatga ttaattgtga ccaaactcaa acttctggca cttggagtgc
     5821 ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg ggcgcaggcg
     5881 taggtgtcgt tgagcaccca tataaagatt acgataccga tgtttaccca gtaccggtaa
     5941 tcaactatga aggcgataac ttctggttcc gtggcttagg tggtggttac tacctgtgga
     6001 atgacgcaac ggataaactt tcaattaccg cttactggtc gccgctttac ttcaaagcga
     6061 aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg taagagcacc atgatggctg
     6121 gtctgtctta tgctcacttt acccagtacg gttacctgcg taccaccctg gctggcgata
     6181 ccctggataa cagcaacggc attgtctggg atatggcttg gttgtatcgt tacaccaacg
     6241 gtggcctgac cgtgactccg ggtattggtg tgcagtggaa cagcgaaaac cagaacgaat
     6301 actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggg tataacccga
     6361 atgacagctg gagcccttac cttgagctga gcgccagcta caacttcctc ggcgactgga
     6421 gtgtttacgg taccgcgcgc tacacccgtc tgtctgatga agttactgac agcccgatgg
     6481 tggataaatc ctggactggc ctgatttcta ccgggatcac ctacaaattc tgataatgca
     6541 tctttccagg gcattttgtg cataaaaata gggcgtcacg ccctgttttg cattacggtg
     6601 gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt caatttagtg gcgatgtctc
     6661 actgccagcc atagggcagg gaacatggta tatgggcgaa gatgccagtc agcgcaaaac
     6721 agaagttgct gcactacgcg caggcattga actcggttta accctcattg ataccgccga
     6781 aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa gcattaaccg gtctgcgaga
     6841 gaaggtcttt ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa aagcgataaa
     6901 tgcatgcgaa gccagtttac gccgtctcaa tactgattat ctcgatctct acttattaca
     6961 ctggtctggc agtttcgctt ttgaagagac tgttgctgcg atggaaaaat tgatcgccca
     7021 gggaaaaatc cgtcgctggg gcgtttctaa ccttggttat gccgatatgc aggaactctg
     7081 gcagctgccg gggggaaatc agtgtgccac taatcaggtg ctttaccatc tcggttcacg
     7141 agggattgag tacgatctac tcccctggtg ccagcaacag cagatgccgg tgatggctta
     7201 cagtccatta gtccaggccg ggcggttgcg caatggactg ttaaaaaacg cggtagtcaa
     7261 cgaaattgca catgtccaac aaaatgcagc tgcgcttgag gtcgaacttt cgtcagcgga
     7321 attagctatg ctggataagg catatccggc accaaaagga aaaactgcgc tggatatggt
     7381 gtgatgttga tacgtgccgg atgcatcgca cccggcacac aacattaacg caaattaacg
     7441 tttggcaacg cgaatggatt gcgccagatg tgcaggtttc tctttggtga ctttttgcgt
     7501 ttctgaagcg taagccgttt ctacacaaac aaaggtcttg tagccatcgt ccggcatgtc
     7561 gcccatgcta actgaaagcg ccggtcccgg gttccagccg acaacgttca gatgatgctg
     7621 gtggcctacg gcgataatac gattcagcgc ttcatcatta atcacgctgc aatcttgcgg
     7681 attcagatac acgcgatcgg tacggtcagg gaaggtctga ataccgtcgg tcagtacatc
     7741 ttctttcgcg tcattcactt tatcaatgaa gcgatcgccc agcccactga cgcttacctt
     7801 agcgatatca cccacgttaa agtaggtatg cagggcagag gtggtttcaa attcgccatg
     7861 tgattcaaga tcgatttcac aggttttacc cacgcggaaa tgcgctaaca gcgtaaagtc
     7921 gtgcggccag aattttttcg tctcttcgct ttgtgtcaat tcaaaagtca gcgctacgcc
     7981 atcagcatct tcatgatgtg atttcagcgt ccacggcagg ttgcgggcaa aaccgtgcgc
     8041 aggcagacct tgttgtgccg ccggaccaaa ccacggccag caaaccggta cgctaccgcg
     8101 aatagcgacg ccatttttga acggtgtgtt gttgctcaac cacagaactt cttcttcacc
     8161 cgcaggtttc cacgagagaa ggtgtgcgcc ctgtaatgca aaagaggctt ttacctgggg
     8221 atgatcgacc acaatgaggt ccagttcatc cagtttacga cgggagagga caggggagat
     8281 ttgttcgatg accggaaggg caaaaatttt cttaatcatg acgcagtcct ttaacttcat
     8341 tttatcaggt aaaaaaaaga gcgaccgaag tcgctctttt tagatcacag tgtcatctca
     8401 acttatttgg agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg
     8461 tcgtaccaag ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg
     8521 aacacggaag tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag
     8581 cccagaacgc ctttcatttc gccttcagca gcagctttaa cggcagcttt gatctgctcg
     8641 taagttgcag ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga
     8701 acgcggaacg ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct
     8761 ttagcagcac cggtagagga cgggattatg ttctgggaag cgccgcggcc gccgcgccag
     8821 tctttgtgag acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc
     8881 atcagacctt cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag
     8941 ttggtggtgc aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg
     9001 cctttaacga acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca
     9061 ccagcggtga tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg
     9121 acaacgtcaa cacccacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca
     9181 cggatttttt taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa
     9241 cggccgtgag tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg
     9301 ttgattgcaa cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg
     9361 atacggccaa aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta
     9421 gtgaataaaa ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg
     9481 tcaatcattg cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc
     9541 ttcctttggg cttgagacca catggggtcg gcgccccgaa ttttaaaggg caattacgat
     9601 aaaaatgtga ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac
     9661 aaaattttgc cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat
     9721 taaagtgaga tgtgagtaaa tggcgaataa accttcggca gaagaactga aaaaaaattt
     9781 gtccgagatg cagttttacg tgacgcagaa tcatgggaca gaaccgccat ttacgggtcg
     9841 tttactgcat aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt
     9901 tcattccgaa accaagtatg attccggctg tggctggccc agtttctacg aaccggtgag
     9961 tgaagaatcc attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg
    10021 ttgcggtaac tgtgatgccc atctggggca tgtctttccc gacgggccgc aaccaacagg
    10081 cgaacgttat tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga
    10141 aatcaacggt tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat
    10201 cttgatgaca ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt
    10261 gaactgggga aatggcctga tggcgttgcg ttgaccgagg aacaaaaaga gaattgcctg
    10321 caactggtga tgctgtggca agcccgccat aataccgaag cacagcatat gacaattgac
    10381 acaaatggtc agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca
    10441 gcaaagccta ttgccatgtt taagtaagtg gttcatcccc tttttgccgc ggctatgaag
    10501 gggatagcca acaatgaccc cacgactcgt ctcaattatc tcgacccgac ctggctaagt
    10561 cccagtccga tctcttgcag tcgcgttcac gcctgaatga cttctcctga acttaagtct
    10621 cagacctatt tggccggtaa tccctctcga atttgctctt tttgcgtccc gccttccacc
    10681 aacctgtaac agacccagcc attttctgcg taattgtgat ctaagttgta aaatgcaggt
    10741 cattatctgt gttgtgcaga tttaatgttg acctcaaaaa tcatgtgggt aagtatgatg
    10801 gccatctaag caaaacaaaa cacagaggat gacgcaatga aagcactggc tcggtttggc
    10861 aaggcctttg gcggctacaa gatgattcat gtcccacaac ccatttgtgg cccggaagac
    10921 gtagtgattg aaattaaagc cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc
    10981 gatagcggtt ctgatgagtt taactctatc cgcggccatg agttcgcagg ttgtattgcg
    11041 caggttggtg aaaaagtcaa agactggaaa gtggggcaac gcgtcgtgtc ggataacagc
    11101 ggtcacgttt gcggtgtttg tccggcctgt gaacagggtg attttctgtg ttgtacagaa
    11161 aaagtaaacc ttggtctgga taacaatacc tggggcggtg gtttttccaa atattgtctg
    11221 gttcctggtg aaattctcaa aattcatcgt catgcgttgt gggaaatccc tgatggtgtt
    11281 gattatgagg acgcagccgt acttgaccct atctgtaatg cctataaatc catcgcgcag
    11341 caatcgaaat tccttcctgg tcaggatgtt gtcgtcattg gcactggccc actcgggctg
    11401 ttttccgtac aaatggcgcg gattatgggg gcggtaaata tcgtcgtcgt tggtctgcaa
    11461 gaagatgtgg cggtccgctt cccggttgca aaagaactgg gggcgacggc agtagtaaat
    11521 ggttctaccg aagatgtggt ggcgcgctgc cagcaaattt gtggcaaaga taatctggga
    11581 ctggtgattg aatgctccgg tgccaatatc gcactgaaac aagccatcga aatgctccgc
    11641 ccgaatgggg aagtggtacg cgttggaatg ggcttcaaac ctcttgattt ctcgattaat
    11701 gacattaccg cctggaataa aagcatcatt gggcatatgg cctatgactc cacctcatgg
    11761 cgtaacgcta tccggctatt agccagcggc gctatcaaag tcaaaccgat gatcacgcat
    11821 cgtatcggcc tgtcgcaatg gcgcgaaggg tttgatgcga tggtcgataa aaccgcaatc
    11881 aaagtgatca tgacttacga ctttgatgaa taactactct cctgaaggaa cgttatcgca
    11941 tggaacagat aacaaaaccg cattgtggtg tacggctgga tcgcttaccg gattgccgct
    12001 ggcattcgtc aatgtttgcc atcgtcgcgt ttggcttgct tgtctgctgg agtaatgccg
    12061 ttggtggctt gatcctcgcg cagctgaaag cgttgggctg gacagataat tccaccacag
    12121 ccacattctc agcaatcacg accgccggaa tgtttctcgg tgcgctggtg ggcggcatca
    12181 ttggcgacaa aaccggtcgc agaaatgcgt tcatcctcta tgaggccatt catattgcct
    12241 cgatggttgt cggtgctttc tcaccgaata tggattttct tattgcctgc cgttttgtga
    12301 tggacgttgg gctgggagct ttactggtta cactgtttgc tggtttcacc gaatatatgc
    12361 ccggtagaaa tcgcggaacg tggtccagtc gggtttcctt tattggcaac tggtcatatc
    12421 cgctctgctc actgattgcg atgggactca cgccgctgat tagtgcagag tggaactggc
    12481 gggtacaact gcttattcct gcaatattgt cgcttatcgc tacggcgctg gcctggcgct
    12541 actttcctga atccccgcgc tggctggaat cgtgcggacg gtatcaggaa gccgagaaag
    12601 tcatgcggag tatagaagaa ggcgtcatac gccagacggg taaacctttg ccgcctgtgg
    12661 ttattgctga tgacggtaaa gcgccacaag cggtgccgta ttcagcctta ctgacaggag
    12721 tattactgaa acgcgtgata ttaggctctt gtgtgctgat taccatgaac gttgtgcagt
    12781 acacactaat taactggttg ccaacaatat tcatgaccca ggggattaat ttaaaagact
    12841 cgattgtttt aaataccatg agtatgtttg gcgcgccatt tggtattttt attgccatgc
    12901 tggtgatgga taaaattccg cgtaaaacaa tgggtgtggg gctattaatc ctgattgcgg
    12961 tgctcggata tatctattca ctgcaaacca gtatgttgct cataacgctg attggtttct
    13021 tcctgattac tttcgtctat atgtacgttt gctatgcctc ggcagtgtat gtccctgaaa
    13081 tctggccgac agaggccaaa ctccgtggct ccggtctggc gaatgcggta gggcgaatca
    13141 gtggtattgc cgcaccttat gccgttgcag tgctgctcag tagttatggt gtaacgggag
    13201 tctttattct tctgggggct gtttcaatta ttgtcgcaat tgctatcgcc accattggaa
    13261 ttgaaaccaa aggtgtctcc gttgaaagtt taagtattga tgcagtagcc aataaataat
    13321 acgggtggat tcaaaatgaa aaattcaaaa gcaatattgc aggtgccggg cacaatgaaa
    13381 attatttcag cagaaatacc agtgcctaaa gaagatgaag ttttgattaa agtagaatat
    13441 gtcggtattt gtggttcaga tgtacatggt tttgaatcag gcccgtttat tccgcctaaa
    13501 gacccaaatc aagaaattgg cctgggtcat gaatgcgccg ggacggttgt ggctgtggga
    13561 agccgcgtgc gcaaatttaa accgggggat cgggtaaata tcgaacctgg cgttccttgc
    13621 ggtcactgtc gttactgtct ggaaggcaaa tataacatct gcccggacgt tgattttatg
    13681 gcgacacaac ccaactaccg cggcgcatta acgcactatc tgtgtcatcc ggagagcttt
    13741 acttacaaac tgcccgacaa tatggacacg atggaagggg cgctggtgga gcctgccgca
    13801 gtcgggatgc atgccgcgat gctggcagat gttaaaccgg gtaagaagat aattattctg
    13861 ggagcaggtt gtattggttt gatgacgttg caagcgtgca aatgcctggg cgcaacggaa
    13921 attgccgtcg ttgatgtact ggaaaaacgt ctggcaatgg gggaacagct tggtgcgaca
    13981 gtggttatta atggcgcaaa agaggacact attgcacgct gtcagcaatt taccgaagac
    14041 atgggcgcag atattgtttt cgaaacagcg ggttctgcgg tcaccgttaa acaggcacct
    14101 ttaatctggt aatgcgcggc ggtaaaatta tgattgttgg tactgtaccc ggcgattcgg
    14161 caatcaattt cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca
    14221 atcgttatcc ggtcacgatt gaagctattt cttcagggcg attcgatgtg aaatcgatgg
    14281 tgacgcatat ttacgattat cgggatgtac aacaggcaat ttgaagagtc agttaacaac
    14341 aaacgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    14401 acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacat tatgcaattg
    14461 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    14521 cgaaatcccc ggttattatt tcttttggta caggttttgt cggtaacacc tcatttgaag
    14581 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    14641 gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt atgaattcat
    14701 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    14761 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    14821 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    14881 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    14941 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    15001 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    15061 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtcg
    15121 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    15181 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    15241 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    15301 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    15361 accggggggc gatgcaataa atgaagcaac aattatttct cgtctggggc atcgtaccgc
    15421 attaatgagc cgcattggta aagatgcctc agggcaattt attctcggcc attgtcgcaa
    15481 agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat ctattaacgt
    15541 tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg
    15601 gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat tattatcact
    15661 ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa tttttacgca
    15721 agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac
    15781 gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa attttgccga
    15841 ggcaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct ttcttgcgtg
    15901 cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca agcgtggtga
    15961 catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca ttggcgcggg
    16021 cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg
    16081 cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg ccaccaccgg
    16141 cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat aatgaaggca
    16201 aatgaaaaag atacctttag gcacaacgga tataacgctt tcgcgaatgg ggttggggac
    16261 atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa tatgtattga
    16321 tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc taggatataa
    16381 ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt
    16441 tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt
    16501 tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag aggtagaagc
    16561 cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact ggcagtcggt
    16621 gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt taaaggctga
    16681 agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct
    16741 gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc gggcaatgga
    16801 aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt attccccgct
    16861 agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg gcgctcgggc
    16921 aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg
    16981 gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg cgtggatatt
    17041 aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac aggtacgcga
    17101 aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga tgagggaaat
    17161 ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct gtgaaaactg
    17221 tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt atgcgataat
    17281 cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc
    17341 aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat ttgagtggga
    17401 tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa gatgaaggct
    17461 ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg gagaatatcc
    17521 atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca cgcaaggcac
    17581 taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc gtaatggaat
    17641 tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg gcggaagcga
    17701 ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa
    17761 atacactttc cctgcaagga agaattacta aagagatcat cagccgctac catgtcgata
    17821 tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac tctaatgaag
    17881 cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg ttattggttg
    17941 atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt catattaatt
    18001 atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa gacaataata
    18061 tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct gcgcctggaa
    18121 ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct tttatagcaa
    18181 atctgaggca cccaatggaa caatatgatc aaattggcgc aagactggac cgcttgcctt
    18241 tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta ttaacggggt
    18301 ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga tggtcaaata
    18361 atttcctcaa tgccgccttt accccggcat taatgtttgg ttatttcatc ggctcactta
    18421 ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata aatcttctca
    18481 tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg ctcattttct
    18541 tgcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat gcctcattta
    18601 cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca tttgttggta
    18661 actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt tttagttggc
    18721 gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc tcaggtaaat
    18781 actttatcga gtcgccacga tggctggcag ggaaagggca aatcgccggt gcagaaagcc
    18841 aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta ccccagctta
    18901 ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg ctcctgttta
    18961 aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca atgaacattt
    19021 cgctttatac cattaccgta tggataccga ccatatttgt taactccggc attgatgtcg
    19081 ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga atatttattg
    19141 cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta cttattatta
    19201 ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt ttaatctatg
    19261 gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc
    19321 cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat gccgtcggac
    19381 ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat tatgggtcga
    19441 tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt
    19501 ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat taaatctttc
    19561 gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct acaatgccag
    19621 tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct gccagcgtat
    19681 atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc tggggctgga
    19741 tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac tgtaacgcgt
    19801 ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc aattcatcga
    19861 tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa
    19921 aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct gcgatctctt
    19981 tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc
    20041 agaaagtttg ccgcaggccg tcgagttggc ctggcgtata aggctctacg ccgtgctggc
    20101 tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc gcttctccgc
    20161 gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca ccttccggta
    20221 cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac agggcgcgat
    20281 ggggcattgc agtctcctta atcatccggc gtcagttcgc cgcgcaggtt ttggctcatg
    20341 gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag tttggttagc
    20401 gtggcttcta cagtcatatc tgcaccgcca ataacgccgg catgggcgag gacgttaccg
    20461 gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt gaccaccaca
    20521 ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta
    20581 cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg
    20641 acgtcagcag aaatccctgg ataaatcgtc actacgccaa ttggttgtgg cgtgattgga
    20701 tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg acgaatatgg
    20761 ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa accatcggca
    20821 tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag cgttacttcg
    20881 ttgatcggat aattcgccgc aacgtacaac gcattcagca gattaatttg tccgtcagag
    20941 cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc gagattctcg
    21001 agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca
    21061 aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg ccagtcttcc
    21121 ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa atctggcatc
    21181 tccaggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc tgacaccgga
    21241 atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt
    21301 gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta tatacgtgag
    21361 gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga cagcgggaag
    21421 aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc agtacaagac
    21481 ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc
    21541 ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa
    21601 catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt tgtccatcac
    21661 tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt tcacttttgc
    21721 gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga ccagcccgtt
    21781 agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa tttgctccgg
    21841 cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat tctcaatgct
    21901 taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa catccgccag
    21961 cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat tttctacggt
    22021 ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag ccacaatgta
    22081 attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca tcgatacaac
    22141 cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt cagacgcggt
    22201 aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg ggtcaaggcg
    22261 agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag tttcctcgcc
    22321 atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat ctgccggcgt
    22381 tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct tactccagcc
    22441 gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat cgaccagctt
    22501 gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct caagcaaccc
    22561 ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa cagtattcag
    22621 atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg ccggtgacat
    22681 atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga acacatgggt
    22741 ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag caaaaccgtg
    22801 cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg cgagataata
    22861 ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt cacgaaactc
    22921 tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga agtttttcag
    22981 atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat
    23041 atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct ggcggcttaa
    23101 tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa tgtccagcag
    23161 cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca tccagatccc
    23221 cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac ggacgaaatt
    23281 cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta
    23341 ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat cgtaatgacc
    23401 caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt
    23461 aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg aactattgat
    23521 caatcgccgt agcgcctccc gcttggctga acccgcgcca acgggtgaac aactgcaaaa
    23581 catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt
    23641 tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg gggcgattgc
    23701 tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct
    23761 catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat gggaacagga
    23821 aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc agggatttgg
    23881 cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag cattcggttg
    23941 ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt tgaaagcatc
    24001 tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat aattgtcgcg
    24061 aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt atcacttcac
    24121 ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag cgagaactcg
    24181 attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc cccaaaagtg
    24241 ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt gcttgtgggt
    24301 aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag cgttatcagt
    24361 accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg cattgcggcg
    24421 actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc gattgcgatc
    24481 ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac cgaaggtgga
    24541 cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat cgatgcgccg
    24601 gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac
    24661 agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat cggcgttctt
    24721 accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc gacggaagtg
    24781 atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg
    24841 accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca gggggctggt
    24901 gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga agagtacatt
    24961 aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg tcatctgatg
    25021 ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg
    25081 ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc cgagtttggc
    25141 attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag
    25201 atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc
    25261 tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg gtaatggtca
    25321 ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg cctacgacag
    25381 ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt ggcaattaca
    25441 gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag
    25501 cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg atgaagtgct
    25561 ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct tgataaacga
    25621 cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt
    25681 gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat
    25741 gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac tttccgtggg
    25801 acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt
    25861 cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg atgagcggtt
    25921 taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt
    25981 gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac cggctattgt
    26041 caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt cgctttcggc
    26101 gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg
    26161 ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt gtcgctattt
    26221 gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc
    26281 ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt gggatgacaa
    26341 aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga tcaacctgac
    26401 ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc agttctgccc
    26461 ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca aatttgtcgc
    26521 taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca aagagcgcga
    26581 tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga
    26641 aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg atgcaacact
    26701 gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct
    26761 tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg aactgctgtt
    26821 caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg ccggaaaagc
    26881 gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc actgggaatc
    26941 ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc agccgctggt
    27001 ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg
    27061 cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg
    27121 tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag cgtaagcgag
    27181 cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa gaaaattcgt
    27241 tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa cagggaatgt
    27301 gatgaaaaaa gttttattac aaaaccatcc tgggagcgag aagtattctt ttaatggctg
    27361 gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca tgctgctttg
    27421 taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg cagcgataac
    27481 atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt
    27541 aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactacatta
    27601 ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat
    27661 cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa
    27721 gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat
    27781 catcagtaaa agacgcgagt ctttagagaa tcaatggggt atcgaagata gcgaaagtta
    27841 ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa
    27901 catggaggaa gccccagagc aggttatcgc cgtgttgaat aaatttgctg tttttccctc
    27961 agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg
    28021 ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat
    28081 tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt
    28141 cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg
    28201 ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca
    28261 gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc
    28321 atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg
    28381 cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat aagcgtagcg
    28441 ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac
    28501 aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc
    28561 aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc
    28621 tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc
    28681 agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc
    28741 ttcgatggtg gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag
    28801 ctgatgcgcg gtgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac
    28861 cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc
    28921 tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt
    28981 gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc
    29041 aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac
    29101 gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
    29161 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
    29221 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
    29281 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
    29341 cagttcagtc atcagcgcct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
    29401 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
    29461 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
    29521 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
    29581 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
    29641 cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag
    29701 tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg
    29761 gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa aaaccttata
    29821 tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac
    29881 catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag
    29941 agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt
    30001 tatcagtagc agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt
    30061 agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt
    30121 aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg
    30181 ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg aaggaatact
    30241 gctgcaatgc cagcgtgatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt
    30301 aaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata ttgcagcgcc
    30361 tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag
    30421 tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg
    30481 gcaacatact cacccacagt tgcttcgatg tccaattctt cgtttaactc acgcaccagc
    30541 gcctgctgct ggctttcatc cagctcgact ttacctccgg caaactccca taatcccgcc
    30601 tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg
    30661 gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa
    30721 tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg
    30781 ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc
    30841 gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc
    30901 gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct
    30961 tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg gcggctttct
    31021 tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc
    31081 accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag
    31141 cagttttctc gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc
    31201 acataagtcg ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt
    31261 tgtgttggga tgtttattcc ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc
    31321 gtggtttgcg tggtttgcgt ggtttgcgtg gtttgcgtgg tttgcgtgga tcttcggtgc
    31381 gttgtgctgg atgacgacct ttacgcgggt gtggagtggg tatctgacgc tgaagtcgct
    31441 ccagcgtcag taattgcaat gttatttgct actgcccggg ccgcgttcac cggttgcaac
    31501 cggatttttt ggatcgctgc tccattcgta ccagccgccg tcatacacgg aaacattctt
    31561 ccaacccatg gcgcgtgcgt acataaaggt ttcggacgcg cgccagccgg tgccgcagta
    31621 gaatgaaact tgctgatctg gtttgatatt ccatgctttc cacatagcgg taatatcatc
    31681 ggcgctacgc atggtgccat ccgggttatg gaaatcttcc atatgcgtcg agtcgctacc
    31741 agcgtgtccc caacgtgctc cggctatttc accttttggt ttaatatagc tgtaaccgct
    31801 ggtcgtaccg ataaattctg gccacgaacg aatgctcacc agcgatgcat cctggcgatg
    31861 cagcagtcca cgggcctgtt ccatatcgag catcaactgc ggttgtgccg ggatcttcac
    31921 gccgaaatct ggttccgctt tcactttcgg tggcgttcct cgctcaacag gcagtcccgc
    31981 gtcggaccag gtttgccagc cgccatccag cagacgcaca tctttcacgc ctgcgtagag
    32041 cataatttgc gccacacgcg ctccagcgta tacgtcacgc ccgtacagaa tgaccgtggt
    32101 gtcatggcga atgccgtgtt ttgccagcat cgcttttagt tgttcatcag aaactttgtt
    32161 ccacagcggt tcgctttcca cttcgttggt atcgatgtag tcagcgccag gaatatggct
    32221 gataaggtaa agcttaggag cgccccaggc cgcttcaatg actttccagc caccgacagg
    32281 tttcgccgta acctctttac cttgttgcag gtcatgcagc cattgcggat aaaccagctg
    32341 ctcaaagtgc ggcagttttt gtagacggga aggttcgctt agcgcgtcac tcagaatgga
    32401 gatatgcgta aaacctgctt tttgcagtcg cgttttgacg gcgtcgacat ctttgccatt
    32461 accgtacagc gccaccgggg catcggcttt caggttatgt tgcttgatcc acgcgttgag
    32521 ctgttcggtg ctcattttgt cgagccagct agcagagagg tttaaggcgg caggttcatg
    32581 accagaaggg ccatttaagg tttgtggcca gccgttataa aacgcgctgg ggcgagtatc
    32641 tatcgctttg ccattttgtt gctgaagttg gtcaagtgta agaggcttcg ccagttcagc
    32701 ggcctacgaa gaagcgcaag ccagccctaa agccattgcc agcgcggtca tttgagaaac
    32761 acgtttcatc gaaaatcccg acattatagt tttgagtgcg ccattctgaa ctgacgcatt
    32821 gagaaaaact ttgcgttatc gcagtttgtt gtaattttct gaccactgcg ccatatccag
    32881 aacagaacta tcagcaggaa catcctggag atcgtgcgtt acctgaacga cggggatcgc
    32941 cagggcgcga acttcgctga acacccactg gcgaaaatta tcgcgcagag ccacatcaag
    33001 acggctgaat ggctcatcca ggagtaacgc ttttggctgg gcgagaaggg cgcgtagtag
    33061 agcaacgcgc gctcgctgac cgccagacaa agtggcagga tcctgatgga aagctccgtc
    33121 aaggcctgaa cgctcaagtg catcattcac ggcattacgt cgggcattcc ccttaagtgt
    33181 cgccggtagc gccagcagta aattttgccc gacgctgaac tggtcaaata acagtgcatc
    33241 ctgaaaaaga atgccaatct gacgctgtgc ggtgggtagc atgtcaatcc gttgctcatt
    33301 gagccatagc tcacctgtac aagaaaactg tccggccagt gcaccaatca tccatgaaaa
    33361 cagagtggat tttccacagc cagatggccc cattaacgtg acgatgtcac ctttatcaac
    33421 cgtaaagtta acgtttgtca gcaagcggct ttctggtaaa cgtagcgaaa catttttcac
    33481 gcagagcatt agcggattcc ttgtctgaca taacctaccc attttgcgac taacgcggtc
    33541 agggcaaaaa taataagcgg taacaacagt tgccataaag cctgggcggc gagaataccg
    33601 ttgctgccgc cgctgcttaa tgccaccgcc tcagtggtga gcgtcggaaa acgccccgcg
    33661 cccagccaca gcgtcggcat atactgcgca atactgactg aaaatcccac cgcgaaggca
    33721 atcagcacag ggcgcaacat gagtgggcat ttcacgtaaa agaagatttt ggcccgcgac
    33781 cagcccagtg tttgcgcaat caaaattaac cgtgaatcaa tgcgctgcca ggcgggttgc
    33841 aggataaaca gcatccacgg cattacccac agcagatgcc cccagaccac cgccgtccag
    33901 cttccatcca ggttcagcca tagcgccagc gtgtactggc ccgccaccag tggcagagcg
    33961 ggcaatagaa tgggcaacca tagccacaac tggcgacgct gtgcccccca ttccagccac
    34021 agcagtaaca ggagcaaagc gatgaatgtt gctaccagcc ccattgtcag actgttgatg
    34081 agtgcttcac tattgatcgt cgactgatcc gcgaggatcg ccagcagaac cacacacagc
    34141 acaccggtta agggtaaaaa actcgccagc gtagtgcctg gtaataaagg tgtggcaggc
    34201 ttgcgaatgc catctacgcg gggaatagtg cgccgccagc tgcgccatag cagatagctc
    34261 agcaaaacgt aggcggcgag taacagcatc agcagcagac tggcgagtgc gcctttcgtt
    34321 tgttgatccg cgttgccctg agttaaccac tgccagctaa ttactgccag cgtcggtgga
    34381 ttacctggcc caagaataat cgccacatcc acgaccgaaa gcgaccaggc aacaatcgcc
    34441 agcatcgcca ttgccagcga aggcgcgacg gagggcaaca gcagccagtt caggcattgc
    34501 cagcggctgt agcccagcga atccagcaca atgacctgct gcaacagccg tttttcgctc
    34561 aatactgcca ctaagatcca cagcagaaat gcgctttctt tcactgcaag ggtgaggccc
    34621 agaccgatcc aaatcggtcc attggcggag tgaaatacgg gaaatagtca tatagcagcc
    34681 ctccgtcagc aaagagcagc agggcgctgg tggcaaaagc cacatgggga atggcgagca
    34741 gccacggcag acgggcgcac atacgctgcc atttcggccc aggccatagc gccacaatca
    34801 ccagcagggc aatcaacaat gccccgaccg ccgcgatggt tgtcgacacc agcgttgcca
    34861 gtaatgcctg cggtaactgt ggatcggcaa ataacgcctg ccagcgtgtc aacgacaacg
    34921 caggcgatat cagcgtgaga gctgccggga tcagcggtgc ataaatcacc gccaccatcg
    34981 cccacagcaa aaagattaat gcgtaccgta acggcgtaac cattcttgtt ccagtgcatt
    35041 cacccatcct gcgtgcggtt cagccagtac cggcggcaga tcctgcggca ttcttgattg
    35101 caatatttcg cgctgcccgt caggcagttt ttgcggatcg agaacagaag gatcgcccca
    35161 gacaacggga tctgctttac gcagttgcgc atcgggtgag agcaggaaat tggcaactac
    35221 cttcgccgca gcactggcat tcgcgttggc tggaatggtg acgaaatgca cgttgccaat
    35281 catcccctcg cgaaagccaa aactgtaact gcttgccggc aaatcgccgc tggcgatttt
    35341 ttgctgcgca tgcgcggggt taaaggtcag cgacaggcgc aatgtgccgg ctttcagcag
    35401 agcatccatc cgcgcgggcg aaggcgggaa atcctttcct tcgcgccaca aatacggata
    35461 cagtgcatca agatattgcc acaagggcgc agtgacacgg gcgaaagtcg catcgtccgg
    35521 cgcttctttt aatgcggcgg gatctggcgt cagcataatc agcaactgtt caagaaacgc
    35581 cgtgccggta aagtccggtg ggcgcggata agtaaccgtg ccgggattgg ctttagcaaa
    35641 ctccagtaag gcttgcggcg tttgtggtgg ctgtggcgta acatcgcggc gggcgataaa
    35701 cgtcagttgt gcgccgcccc agggcgattc cgcaccttgt gtcggcactg aaaaatcttc
    35761 ccgcaccggc agctgtgtgt cgacatagcg ccagttgggc agagtctccg cccagcccgt
    35821 ttgtaataaa ttggcctctt ttaag
//
LOCUS       DADMFL010000030        35255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.030, whole genome shotgun sequence.
ACCESSION   DADMFL010000030 DADMFL010000000
VERSION     DADMFL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.030"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNZ27_001477"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="JNZ27_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY8663859.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /locus_tag="JNZ27_001478"
     CDS             1519..2649
                     /locus_tag="JNZ27_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY8663860.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="JNZ27_001479"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="JNZ27_001479"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY8663861.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="JNZ27_001480"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="JNZ27_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY8663862.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="JNZ27_001481"
     CDS             5719..6660
                     /locus_tag="JNZ27_001481"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY8663863.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6648..7586
                     /locus_tag="JNZ27_001482"
     CDS             6648..7586
                     /locus_tag="JNZ27_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY8663864.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="JNZ27_001483"
     CDS             7680..8930
                     /locus_tag="JNZ27_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8663865.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGGFGFANASLESIFGYVLVPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9001..9699)
                     /gene="yeiL"
                     /locus_tag="JNZ27_001484"
     CDS             complement(9001..9699)
                     /gene="yeiL"
                     /locus_tag="JNZ27_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY8663866.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9830..10771
                     /gene="rihB"
                     /locus_tag="JNZ27_001485"
     CDS             9830..10771
                     /gene="rihB"
                     /locus_tag="JNZ27_001485"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY8663867.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10871..12121
                     /locus_tag="JNZ27_001486"
     CDS             10871..12121
                     /locus_tag="JNZ27_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8663868.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFGVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGCVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12177..13265)
                     /locus_tag="JNZ27_001487"
     CDS             complement(12177..13265)
                     /locus_tag="JNZ27_001487"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8663869.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13268..14125)
                     /gene="nfo"
                     /locus_tag="JNZ27_001488"
     CDS             complement(13268..14125)
                     /gene="nfo"
                     /locus_tag="JNZ27_001488"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY8663870.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14199..15248)
                     /locus_tag="JNZ27_001489"
     CDS             complement(14199..15248)
                     /locus_tag="JNZ27_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY8663871.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15347..16228
                     /locus_tag="JNZ27_001490"
     CDS             15347..16228
                     /locus_tag="JNZ27_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8663872.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16434..17903
                     /gene="lysP"
                     /locus_tag="JNZ27_001491"
     CDS             16434..17903
                     /gene="lysP"
                     /locus_tag="JNZ27_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY8663873.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18195..20186
                     /gene="cirA"
                     /locus_tag="JNZ27_001492"
     CDS             18195..20186
                     /gene="cirA"
                     /locus_tag="JNZ27_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY8663874.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20218..21054)
                     /gene="yeiG"
                     /locus_tag="JNZ27_001493"
     CDS             complement(20218..21054)
                     /gene="yeiG"
                     /locus_tag="JNZ27_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAY8663875.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="JNZ27_001494"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="JNZ27_001494"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY8663876.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23154
                     /locus_tag="JNZ27_001495"
     CDS             21997..23154
                     /locus_tag="JNZ27_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY8663877.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23296..24264
                     /gene="galS"
                     /locus_tag="JNZ27_001496"
     CDS             23296..24264
                     /gene="galS"
                     /locus_tag="JNZ27_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY8663878.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNTNAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            24615..25613
                     /gene="mglB"
                     /locus_tag="JNZ27_001497"
     CDS             24615..25613
                     /gene="mglB"
                     /locus_tag="JNZ27_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY8663879.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25674..27194
                     /gene="mglA"
                     /locus_tag="JNZ27_001498"
     CDS             25674..27194
                     /gene="mglA"
                     /locus_tag="JNZ27_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY8663880.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27210..28220
                     /gene="mglC"
                     /locus_tag="JNZ27_001499"
     CDS             27210..28220
                     /gene="mglC"
                     /locus_tag="JNZ27_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY8663881.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28256..29491)
                     /gene="preA"
                     /locus_tag="JNZ27_001500"
     CDS             complement(28256..29491)
                     /gene="preA"
                     /locus_tag="JNZ27_001500"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY8663882.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29485..30724)
                     /locus_tag="JNZ27_001501"
                     /pseudo
     CDS             complement(29485..30724)
                     /locus_tag="JNZ27_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(30918..31157)
                     /locus_tag="JNZ27_001502"
     CDS             complement(30918..31157)
                     /locus_tag="JNZ27_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY8663883.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31160..31879)
                     /gene="sanA"
                     /locus_tag="JNZ27_001503"
     CDS             complement(31160..31879)
                     /gene="sanA"
                     /locus_tag="JNZ27_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY8663884.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32029..32913)
                     /gene="cdd"
                     /locus_tag="JNZ27_001504"
     CDS             complement(32029..32913)
                     /gene="cdd"
                     /locus_tag="JNZ27_001504"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY8663885.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSAEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33043..33738)
                     /locus_tag="JNZ27_001505"
     CDS             complement(33043..33738)
                     /locus_tag="JNZ27_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY8663886.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNTMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33735..34133)
                     /locus_tag="JNZ27_001506"
     CDS             complement(33735..34133)
                     /locus_tag="JNZ27_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY8663887.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34372..>35255
                     /gene="dusC"
                     /locus_tag="JNZ27_001507"
     CDS             34372..>35255
                     /gene="dusC"
                     /locus_tag="JNZ27_001507"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY8663888.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8609 c         9307 g         8677 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ctacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg gggctgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgtatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacttggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6661 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcaaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggcgggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggtacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcatcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatt gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     8941 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9001 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgtca gtcctgacaa
     9061 ctgctttctg tttttaatta gatatccttt cttgctttta attaataaac cgtcgtgaat
     9121 gaattgcgcg agaacatata aaagatgtcg ataagaaacg cctaaatact ctgccgcttg
     9181 cgtatgcttt tcgtgataaa gatcaccttc ttgtgagagt aatataaatg ctgccaggcg
     9241 attcattaac ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg
     9301 acttaaggtg acgcaaagtt ttcgtaaaaa tagggtgtcg tttaataaca gcggacggta
     9361 atgtttcata gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc
     9421 atggtctttg tcgattaact caatctcgcc aataaaacag ggagcagcaa agaaatcgat
     9481 cagcgacacg cgaccattag caagcgtggc gtaaagcctg gcgcggcctc gcgtcagata
     9541 aaacagccat gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa
     9601 cagccgcgta tcggctgaaa catccgttga aaagcaatcc ttaaacgcag attcactcat
     9661 caattgctgc ttaaggtcat tattgtggat ttctttcacg atgtttatcc gctatgagat
     9721 ttctcatatt tagcatacgt acgctgttgt taagattcaa tcatcagccc ggtcgaattt
     9781 tctttgctgt agaaaaaaat aacgcaactg gaaacagagg aaataaacaa tggaaaagag
     9841 aaaaattatt ctggattgtg agccgggtca tgatgatgct attgcaatga tgatggcagc
     9901 gaaacatccg gcaatagatt tattaggcat cactattgta gcgggtaatc agacgcttga
     9961 taaaacatta attaatggcc tgaatgtttg ccagaaactg gagattaatg ttccggttta
    10021 tgcggggatg ccgcaaccca ttatgcgtaa acaaatcgtt gccgataata ttcacggtga
    10081 aaccggactg gatggcccgg tattcgagcc gctgacccgc caggcagaaa gtacccatgc
    10141 ggtgaaatat atcatcgata ccctgatggc aagcgatggc gatatcactc tggtgccggt
    10201 tggtccgctt tcaaatatcg cggtggcaat gcgtatgcaa cccgcaatcc tgcccaaaat
    10261 ccgtgaaatt gtgctaatgg gcggcgctta cggtacaggc aacttcaccc catctgccga
    10321 gttcaatatc tttgccgacc cggaagccgc ccgcgtggtg ttcacctccg gcgttccatt
    10381 agtgatgatg ggcctcgatc ttaccaacca gaccgtttgc accccggatg taattgctcg
    10441 gatggaaagg gtaggcggcc ctgccggaga gctgttcagc gacatcatga acttcactct
    10501 caaaacgcag ttcgaatact acggcctggc tggcggcccg gtgcatgacg ccacctgcat
    10561 tggttatctg atcaaccctg atggcattaa aacccaggat atgtacgtcg aagtggacgt
    10621 caacagcggc ccttgctacg ggcgtacggt ctgcgacgaa ctgggcgttc ttggcaagcc
    10681 cgctaatacc aaagtcggca tcactattga taccgactgg ttctggggct tagtcgaaga
    10741 gtgcgtgcgc ggctacatca aaacccatta agtgttgata acaagccggg acagtatcgc
    10801 aagatgcagg cccggctatt tctgatgggc agaaagccgt cgcccaaaat gacatgaaga
    10861 gataatcact atggatgtca tgagaagtgt tctgggaatg gtggtactgc tggcaattgc
    10921 gtttttactg tcagtaaaca agaagaagat cagcctgcgt accgttggcg cggcgttagt
    10981 gttacaggtc gtgattggcg ggattatgct ttggttaccg ccagggcgtt gggtcgcaga
    11041 aaaagtcgct tttggcgtcc acaaagtgat ggcgtacagc gacgcaggta gcgcatttat
    11101 cttcggttct ctggtcggac cgaaaatgga taccttattt gatggcgcag gatttatctt
    11161 tggtttcggg gtattaccgg caattatctt cgtcactgca ctggtgagta ttctctacta
    11221 catcggtgtg atggggatct taattcgcat tctcggcggt atcttccaga aagctttaaa
    11281 tatcagcaag atcgagtcat tcgtcgcggt caccaccatt ttcctcgtgc aaaacgaaat
    11341 tccggcgatc gtcaaaccct ttatcgatcg tctgaatcgc aatgaattat ttacagcgat
    11401 ttgtagtggc atggcctcga ttgctggttc gacaatgatt ggttacgccg cactgggcgt
    11461 acctgtggaa tatttgctgg cggcatcgtt aatggcgatc cctggcggga tcttgtttgc
    11521 ccgcctgtta agcccggcta cggaatcttc gcaggtttct tttaataacc tctctttcac
    11581 cgaaacaccg ccaaaaagca ttattgaagc cgatgcgaca ggggcaatga ccgggctgaa
    11641 aatcgccgcc ggtgtagcga cagttgttat ggcatttgtc gccatcattg cgttaattaa
    11701 cggtattatc ggctgcgttg gcggctggtt tggttttgaa catgcctcgc tggagtccat
    11761 tttaggttac ctgctggccc cacttgcgtg ggtaatgggt gttgactgga gtgatgcaaa
    11821 tcttgccggg agtttgattg gacagaaact ggcaataaat gaatttgtcg cttatctcaa
    11881 tttctcaccc tatctgcaaa cggctggcac tctggatgct aaaaccgtgg cgattatttc
    11941 cttcgcgttg tgcggtttcg ctaactttgg ttctatcggg gtggtggtgg gggcgttttc
    12001 tgcggttgcg ccacaccgtg cgccggaaat cgcccagctt ggtttacgcg cgctggcggc
    12061 ggcgacactt tctaatctga tgagtgccac cattgccggg ttctttattg gtttagcgta
    12121 gtgtgcaatt tatgagccgg attagatgcg gtcaagcgtc gaatccggca acattgttac
    12181 accattggca ctaatgacag cgcgttatcg gcagacaggg tagggttgtt taagctgccg
    12241 ctggcgcgtg aaattgccgc gcaggccatc gcaaaacggg cgctgtcacg gaaattgttt
    12301 ccctccagaa agctatatac cagacccgcc ataaaaccat cgtcagcacc aaaactgtct
    12361 accgtcgtat gcgctggcac agtcagcaaa aattgttcac catccttttc gctgcaataa
    12421 actgactcat cgggcaaata aacaaacagt tgctgaacac cttgctgatg caatgcattc
    12481 actgcggcat tacggtcagc atcgctggtg atcgcctgtc cccataaaat ttccagctcc
    12541 ggtaaagtgg gtttcagggt gtgaatatgc gccagccagt gtttgatttt gcccgctttg
    12601 aattctgaaa cggtatcgac aaacaccggg atctcatcag cgagggtaaa gacccattcc
    12661 agcgcctcgg ctgtcaggtt gcaatcaacc agtaccacgc ccgcatgacg aagtaaatcg
    12721 cgcgacccgt tcagtagttg cggtgtcaac tgttccagca gatgggtatc gttaatcgcc
    12781 agcacggttt gatcgtctcg attggcaatt gccagatacg tcgatgtgct ttgaccatgc
    12841 aaacgaacgc agccggagac attcacgccg gcacggcgcg tttcttccag gagcatttcg
    12901 ccataaaagt catcgccaat cactgaaagc aaatggacgt cacggcctaa cagcgccaga
    12961 ttgtgggcga tattgcgtcc aacgccgcct gccgaacaat gaattgtacc gggatgagaa
    13021 gccgcttgcg ggtaatggat atccgccatc ccgcgaatat ccatattgat tgtccccacc
    13081 actacgcagt attcctgctc ggtgagaatg taacctttgc ctttaatccg gcctttgcgc
    13141 attaaatcca taatatgcgc cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt
    13201 tcgttctgct gaatcagcgg gttacgccgt aaaattgcaa ggatctcctt ttcccggtta
    13261 ttcatcttca ggctaccgct ttttcagttt gttgcgcttt cagccaggcg atctcttctg
    13321 cccagatatc cgggttgatg gtttcaagga tcagcggaat gccgtcgaaa cggtcgttct
    13381 gcataatcca gcggaacgca tcatgaccga tattgccttc accgaggcta tgatggcggt
    13441 caacgcggct gccaaaggta cttttcgcat cgttaaggtg catcccgcgc agatacttaa
    13501 agccgacaat acgggcaaaa tcagcgaacg ttttttcgca ttcggctgga gtacgcaaat
    13561 catacccggc agcgaaagca tggcaggtat caatgcagac gccgacgcgg gatttatctt
    13621 ccacgccgtc gataatcgcc gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac
    13681 cggcggtgtt ttcaatcacc gctgttacac cttgagtttt atccagcgca atgttgatgg
    13741 attcggcaat acgcgcaagg caatcctctt ctgaaatctg catcagatgg ctgccagggt
    13801 ggaagttgag caaagaaagc cccagttgtt cgcaacgctg catttcatct ataaaggcat
    13861 cgcgcgattt ttccagagct tcagtgaccg gatgtccgag gttgatcaga taactgtcgt
    13921 ggggaagaat ttgcgccgat gtgtagtgat atttttcaca ggcggctttg aattcatcga
    13981 tggtttgcgt cgtgagcggt gcggcacgcc actgacgttg gtttttggtg aacaaggcaa
    14041 acgcggttgc gtcgatttcg gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac
    14101 taacgtgcgc tccaatgtat ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg
    14161 gtaatgatag cgggttaaca ggagcaagaa gtagtggttt atgcgatgac gctttgaatc
    14221 acatagttaa tcgcaccacc accaacaatc agccaggcaa acagtaccag tgccatcagc
    14281 agaggtttcg ccccagcttt tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc
    14341 atcgccattg ccagcaggaa ggtatccagc gttaccagca tgttcaccac gctctgcggt
    14401 aacaggtgga acgagttaaa gatggcaact acgatgaaca agatggcaaa ccacggaata
    14461 gtgattttgc ttttctcgcc gctgttcgcc ccagacagct gtttaacacg cgccgccagc
    14521 aggatgagga acggagccag catcatcacg cgcagcattt tagaaataac tgctgcgttt
    14581 tctgcatccg ggctgatggc atgacctgcc gccaccacct gcgccacttc gtgcacagta
    14641 gaaccaatgt agataccgaa agtttccgga ctaaaccatt gagacatcag cggatatatc
    14701 gccgggtaga ggaaaatcgc gacggtcccg aagataacaa cggttgcaac agccacggtt
    14761 actttactgg cttccgcttt cactaccggc tcagtcgcca gtaccgcggc agcaccacag
    14821 atactgctac cggcaccgat caaccaactg gtgtgcttat ccagaccaaa cactttctgc
    14881 cccaggaagc aagccagcag gaaggtactg gacagcgtca gcacgtcaat gatgatccca
    14941 ctgataccga catcggcaat ttgcgagaac gtcagacgga agccataaag aatgataccc
    15001 agacgtaata aatattgctt ggcaaacagc acaccaccgt cacagctttt ccagatgtgc
    15061 ggatagatgg tgttgcctaa aaccatcccc aacaagattg cgagggtgag ggcactaaac
    15121 ccggcacccg caaccgcggg aatggaacca ccccacaggg cgaccccggt gataactgca
    15181 ctcagggcta accccggaat aaaatgccac agtgtacgat gttgtttctg taaggtgata
    15241 ttcgtcataa ccctctcctt taaccgggca taaggttacg actaactggt ttaaaaataa
    15301 aattgattat atatttataa ttaatcttta taagtggtaa gcgactatgc acatcaccct
    15361 ccggcagttg gaagtttttg cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt
    15421 gatgctggcg ttgtcgcaat cagcagtgag cgcagccttg actgacctgg aagggcagct
    15481 tggcgttcaa ctgtttgatc gcgtggggaa aagactggtt gttaatgaac acgggcggct
    15541 gctctatccg cgagcgttgg cactgcttga acaggcggtt gaaatcgaac aactgtttcg
    15601 cgaagacaac ggcgcgattc gtatctatgc cagtagtacc atcggtaact acattctgcc
    15661 tgcagttatc gcccgttatc gccatgatta tccgcagttg ccgattgaac ttagcgttgg
    15721 gaacagccag gacgtgatgc aagcggtgct ggatttccgc gttgatattg gctttattga
    15781 aggaccgtgc cacagcactg aaatcatttc tgaaccgtgg ctggaagacg agctggtggt
    15841 tttcgccgcg ccgacttcgc cgttggctcg tggtccggtc accttagaac agctggccgc
    15901 tgcgccgtgg atcctgcgtg aacgcggttc cggcacgcgg gagattgtcg attatctgtt
    15961 gctgtcacat ttaccgaagt ttgagatggc gatggaatta ggtaactccg aggcaatcaa
    16021 acatgcggtg cgtcatgggt tgggaattag ttgcctgtcg cgacgtgtga ttgaagatca
    16081 attgcaggca ggcacattaa gtgaagttgc ggtccctctg ccgcgcctga tgcgtacgtt
    16141 gtggcgtata catcatcggc aaaaacacct ttccaacgcg ctacggcgct ttctggacta
    16201 ttgcgatccc gcaaatgtgc cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc
    16261 gatttcgtga cgcagcgcct tcagcatgca ttcgccagaa aagagattgg ctgctttact
    16321 tataatccct gggcgatcat gaaggtgtct tataaccgtg tatttctgcc ggaaggattg
    16381 ccaatcgtct gctacaatcg cgccatcatt tttaagatgg atagcatttt tgtatgggtt
    16441 ccgaaactaa aactacagaa gcgccgggct tacgccgtga attaaaggcg cgtcacctga
    16501 cgatgattgc cattggcggt tccatcggta caggtctttt tgttgcctct ggcgcaacga
    16561 tttctcaggc aggtccgggc ggggcattgc tctcgtatat gctgattggc ctgatggttt
    16621 acttcctgat gaccagtctc ggtgaattgg ctgcatatat gccggtttcc ggttcgtttg
    16681 ccacttacgg tcagaactat gttgaagaag gctttggctt cgcgctgggc tggaactact
    16741 ggtacaactg ggcggtgact attgccgttg acctggttgc agcacagctg gtcatgagct
    16801 ggtggttccc ggatacaccg ggctggatct ggagtgcgtt gttcctcggc gttatcttcc
    16861 tgttgaacta catctctgtt cgcggctttg gtgaagcgga atactggttc tcactgatca
    16921 aagtcacgac agttattgtc tttatcatcg ttggcgtgct gatgattatc ggtatcttca
    16981 aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg cgaagcgccg tttgctggtg
    17041 gttttgcggc gatgatcggc gtagctatga ttgtcggctt ctctttccag ggaaccgagc
    17101 tgatcggtat tgctgcaggc gagtccgaag atccggcgaa aaacattcca cgcgcggtac
    17161 gtcaggtatt ctggcgaatc ctgttgttct atgtgttagc gatcctgatt atcagcctga
    17221 ttattccgta taccgatccg agcctgctgc gtaacgatgt taaagacatc agcgtcagtc
    17281 cgttcaccct ggtgttccag cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag
    17341 ttattctgac ggcggtgctg tcagcaggta actccggtat gtatgcgtct actcgtatgc
    17401 tgtacaccct ggcgtgtgac ggtaaagcgc cgcgcatttt cgctaaattg tcgcgtggtg
    17461 gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc cggtttgtgc ttcctgacct
    17521 ccatgtttgg caaccagacg gtatacctgt ggctgctgaa cacctccggg atgacgggtt
    17581 ttatcgcctg gctggggatt gccattagcc actatcgctt ccgtcgcggt tacgtattgc
    17641 agggacacga cattaacgat ctgccgtacc gttcaggttt cttcccactg gggccgatct
    17701 tcgcattcat tctgtgtctg attatcactt tgggccagaa ctacgaagcg ttcctgaaag
    17761 ataccattga ctggggcggc gtagcggcaa cgtatattgg tatcccgctg ttcctgatta
    17821 tttggttcgg ctacaagctg attaaaggaa ctcacttcgt acgctacagc gaaatgaagt
    17881 tcccgcagaa cgataagaaa taagttttct cccttccttg ctaagccctc tcaaccgaga
    17941 gggctttttc aattccattt ccctgacaaa tcatgcggat ataaaattta acatttggat
    18001 tgataattgt tatcgtttgc attatcgtta cgacgcaatc aaaaaaggct gacaaatcag
    18061 aggctgttcc ggctttctgg gatgatcacc tgcatacaaa taagtccacc gcgatgctgc
    18121 cgtacgcaag gggacgtaaa gaagatgtga gcgataccca ttttattttc gtagttacct
    18181 catggagata tggaatgttt aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg
    18241 ctatttcttg tgcatggcct gtgttagcgg tcgatgatga tggcgaaacg atggttgtca
    18301 ctgcatcttc cgtggaacaa aatcttaaag atgcacccgc cagtatcagc gtcattaccc
    18361 aggaagacct gcagcgaaaa ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg
    18421 gcgtacaact gacgaacgaa ggggataacc gtaagggcgt tagtattcgt ggtctggaca
    18481 gcagctacac cctgattctt gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc
    18541 gccacaatga tttcgatctg aactggatcc cggtcgattc catcgaacgt attgaagtgg
    18601 ttcgtggccc gatgtcgtcg ctatacggct ccgatgcgct cggcggtgta gtgaatatca
    18661 tcaccaaaaa aatcggtcag aaatggtcgg gtaccgttac cgtcgatacc accattcagg
    18721 aacatcgcga tcgcggtgat acctataacg gtcagttctt taccagtgga ccattaattg
    18781 atggtgtgct gggaatgaaa gcttacggca gcctggcaaa acgtgaaaag gatgacccgc
    18841 aaaactcaac gaccaccgat accggagaaa cgccgcgtat tgaaggattc tccagccgcg
    18901 acggcaatgt cgaatttgcc tggacaccga atcaaaatca cgattttact gccggatacg
    18961 gtttcgaccg tcaggatcgt gattccgact cgctggacaa aaaccgcctg gaacgccaga
    19021 actactgcgt cagccataat gggcgttggg attacggcac cagcgaactg aaatcctacg
    19081 gtgagaaagt cgagaacaaa aaccctggca acagcagccc gataacttcc gaaagcaata
    19141 cggtcgacgg caaatacacg ttgccgctga cggcgattaa tcagtttctc acggttggcg
    19201 gtgaatggcg tcacgacaaa cttagcgatg cggtgaacct gaccggggga accagctcca
    19261 aaacgtctgc cagccagtac gcgctgtttg tggaagatga atggcggatc ttcgagccgc
    19321 tggcgctgac gaccggcgtg cgtatggacg atcacgaaac ctacggtgaa cactggagtc
    19381 cgcgtgccta cctggtttat aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga
    19441 cggcatttaa agcaccttct ctgttgcaac ttagccctga ctggacgagc aattcctgcc
    19501 gtggcgcatg taagattgtg ggtagcccgg atctgaaacc agaaaccagc gaaagttggg
    19561 agctggggct ttactacatg ggtgaagaag gctggctgga agggggtgaa tccagcgtta
    19621 tcgttttccg taacgatgtg aaagatcgta tcagcatcag ccgtacgtct gacgtcaacg
    19681 ctgcaccggg ctaccaaaac tttgttggtt ttgagacggg cgctaacgga cggcgcatac
    19741 cggtatttag ctactacagc gttaacaaag ctcgtattca gggcgtggaa accgaactga
    19801 aaattccgtt caacgatgaa tggaaactgt cgatcaacta cacctacaac gatggtcgtg
    19861 atgtcagcaa cggtgaaaac aaaccgctat ccgatctgcc gttccatact gctaacggta
    19921 cgctggactg gaaaccgctg gcgctggaag actggtcatt ctatgtttct ggtcactata
    19981 ccgggcagaa acgcgccgac agcgcgacgg ctaaaacacc gggcggttat accatctgga
    20041 ataccggcgc ggcctggcag gtgactaaag acgtcaaact gcgcgcaggc gtgctgaacc
    20101 ttggcgacaa ggatctcagt cgtgacgact acagctataa cgaagacgga cgtcgttact
    20161 ttatggcagt ggattatcgc ttctgatgag aagatgcccg gcgaaccggg cggactttca
    20221 cttcagtaaa tactgcgcat ggaagcgcag gtgatcctct ataaaagagg cgatgaagta
    20281 gtaactgtga tcatatcccg gctgaatacg cagcgtcatc ggccacgctt tctggcgcgc
    20341 ggcttctgcc aataccgcag gttgcaactg gtcggcaaga aactgatcat tatcgccctg
    20401 atcgataagc gtcgggatcg catcctgcgc gttactggca tacatcagcg cgcaactatc
    20461 ccattccagc catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatacgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ttggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctagctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac gctggatcca gtcacggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg cttaatctgc tggtggctgg tgcgcgatgc gccatcggta
    22381 aaaagcctgt ttaataccgg cgtcatgctt tatctggtgg ggcttggcgt tttgctgtta
    22441 ttggggctga tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt
    22501 ttgtatgaaa aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat
    22561 ggtgttggca acagtttact ggcactgggc gcacagtatg gctggcaact ggcagggatg
    22621 atgctcattg gtgcggcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt
    22681 cactatcgtc gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc
    22741 atcgccctgc aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg
    22801 ccgcgggaac tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc
    22861 tggccgcaat tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg
    22921 ctgaccaact atctattgca aacgctgatt tgtaccacgc ttttttacca cctcggtttg
    22981 tttatgcatt ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat
    23041 atcctcttct ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg
    23101 cgtcagttaa ctttgcgtgc tgccggactg gcaatatcta aaacatcaag ataacgatct
    23161 ggatcacatt cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga
    23221 agtcctgtat tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca
    23281 taacaggtag tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg
    23341 gcaacggttt cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca
    23401 gtaatgaaag ccgtgagtga gctggattat cggccaaaca ccaatgccca ggcgctggca
    23461 actcaggtta gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc
    23521 gcgctggtaa aagcggtgga tctggtcgcc cagcagcatc agaaatacgt gctaatcggc
    23581 aatagctatc atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt
    23641 tgtaatgcgt tgattgttca ctcaaaagca ttgagtgacg atgaactggc acaatttatg
    23701 gataacattc ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc
    23761 gtttgcctgg ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt
    23821 catcaacgta ttggttatct ttcttccagc cacggcattg aagatgacgc catgcgtaaa
    23881 gcaggctgga tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc
    23941 actggtacgc cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat
    24001 ctacaactta ccgctgtatt tgcttataac gacaatatgg ccgctggcgc actgacagca
    24061 ttaaaagata atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt
    24121 cccatcgccc gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg
    24181 gctaaattag ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgcc
    24241 gccactgttt tatgccgacg ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg
    24301 cggcgatcac taactcaaca aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc
    24361 acagtatctt aacaatgtga tagctatgat tgcaccgttt taatgttgta acccgtatgt
    24421 aacagtgaat aatcactttc accgaggtaa tagcgtcata acaacaatta aagccgtttt
    24481 ctggagcgtt accgggcatg gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa
    24541 cacttcatta actctactgc cccgccgagc atttatctca agcactaccc tgcataagaa
    24601 aaaccggaga taccatgaat aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt
    24661 tattcggtgc cgctgcacac gctgctgata ctcgcattgg tgtaacaatc tataagtacg
    24721 acgataactt tatgtctgta gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag
    24781 atgttcagct gctgatgaat gattctcaga atgaccagtc caagcagaac gatcagatcg
    24841 acgtattgct ggcgaaaggg gtgaaggcac tggcaatcaa cctggttgac ccggcagctg
    24901 cgggtacggt gattgagaaa gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag
    24961 aaccgtctcg taaggcgctg gatagctacg acaaagccta ctacgttggc actgactcca
    25021 aagagtccgg cattattcag ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt
    25081 gggatctgaa caaagacggt cagattcagt tcgtactgct gaaaggtgaa ccgggccatc
    25141 cggatgcaga agcacgtacc acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa
    25201 ctgaacagtt acagttagat accgcaatgt gggacaccgc tcaggcgaaa gataagatgg
    25261 acgcctggct gtctggcccg aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg
    25321 cgatggcaat gggcgcggta gaagcactga aagcacacaa caagtccagc attccggtgt
    25381 ttggcgtcga tgcgctgcca gaagcgctgg cgctggtgaa atccggtgca ctggcgggca
    25441 cagtactgaa cgatgctaac aaccaggcga aagcgacctt tgatctggcg aaaaacctgg
    25501 ccgatggcaa aggtgcggct gatggcacca actggaaaat cgacaacaaa gtggtccgcg
    25561 taccttatgt tggcgtagat aaagacaact tggctgaatt cagcaagaaa taagactgat
    25621 tgggcgcagt cgtttactgc gcccttattg tatggccaac aaggtaaaat tatatggtca
    25681 gctcaacgac tccgtcttcc ggggaatact tgttggaaat gagcggtatc aacaagtcct
    25741 ttcctggtgt taaggcactt gataacgtta atttaaaagt ccggccacat tctatccatg
    25801 cattaatggg agaaaacggt gcaggaaaat cgacattatt aaaatgcctg tttggtattt
    25861 atcaaaaaga ctccggcacc attttattcc agggtaaaga gatcgatttc cattctgcca
    25921 aagaggcact ggaaaatggt atttcgatgg tacaccagga gttaaacctg gtattacaac
    25981 gttcggtgat ggataacatg tggctggggc gatatcccac caaaggcatg tttgtcgatc
    26041 aggacaaaat gtaccgcgaa accaaagcga tttttgatga actggatatt gatatcgatc
    26101 cgcgtgcgcg cgtcggcaca ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag
    26161 cgttttccta taacgcgaaa attgtgatta tggatgaacc gacttcttcg ttaaccgaaa
    26221 aagaggtcaa tcatctgttc actattattc gtaaattaaa agagcgcggc tgcggtattg
    26281 tttatatctc gcataaaatg gaagaaatct tccagttatg tgatgaagtt accgtattgc
    26341 gcgacggtca gtggatcgcc accgaaccgc tggcaggact gacgatggac aagatcatcg
    26401 gcatgatggt tgggcgttct cttaatcagc gttttcctga caaagaaaac aagccgggcg
    26461 aagtcatcct cgaggtgcgt aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct
    26521 cgtttgatct gcataaaggg gagatcctcg gtattgccgg gctggtgggg gcgaaacgta
    26581 ccgatattgt tgagacgtta tttggtattc gcgagaaatc ggctggcacc attacgttgc
    26641 acggcaaaca gatcaataac cataatgcca acgaagccat aaaccacgga tttgcactgg
    26701 tgactgagga gcgccgctca acgggaattt atgcctatct ggatattggt tttaactcgt
    26761 taatttccaa tattcgcaac tacaaaaata aagttggttt actggataac tcgcggatga
    26821 aaagcgatac ccagtgggtg attgattcga tgcgggtaaa aacgccgggt catcggacgc
    26881 aaattggttc gctctccggt ggtaatcagc aaaaggtgat tattggtcgc tggctactaa
    26941 cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt
    27001 ttgaaattta tcagttaatt gccgaactgg cgaagaaagg caaggggatt attattatct
    27061 cctctgaaat gcctgagttg ttagggataa cagaccgtat tctggtcatg agcaatggtc
    27121 tcgtttccgg aattgtcgat acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt
    27181 ctttgcacct ttaagattag gggcttccca tgagtgcgtt aaataagaaa agttttctta
    27241 cttacctgaa agagggcggt atttacgtcg ttcttttagt tttgctggcg attattattt
    27301 tccaggaccc aacattttta agtctgttga acttaagtaa tattctcacc cagtcatcgg
    27361 tgcgtattat tatcgcgctc ggtgtggcag ggttaattgt cacccagggg accgatcttt
    27421 ctgctggtcg tcaggtaggg ctggcggcag tggtggctgc gacattattg cagtccatgg
    27481 ataacgccaa caaagtgttc ccggaaatgg cgacgatgcc gattgcgctg gttattctga
    27541 ttgtctgtgc cattggtgcg gtgatcggtt tgatcaacgg tctgattatc gcttatctca
    27601 acgtgacgcc gttcattacc acgctcggca cgatgatcat cgtctatggc atcaactcgc
    27661 tctattacga ctttgtcggg gcgtcgccaa tttctggttt tgacagtggc ttctctacct
    27721 ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc ttacatcacc ttctacgcgt
    27781 tgattgcggt ggcgttcgtc tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt
    27841 ttgccattgg cggtaacccg gaagcggcaa aagtatctgg tgtcaacgtc ggcctgaacc
    27901 tgctgatgat ctacgcgttg tctggcgtgt tctatgcctt tggcgggatg ttagaagccg
    27961 gacgtatcgg ctctgccacc aacaacctcg gctttatgta tgagctggat gctatcgcgg
    28021 cgtgcgtggt aggcggtgta tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga
    28081 ccggggtaat tatttttacc gtcatcaact atggcctgac gtatatcggc gtaaacccat
    28141 actggcagta catcatcaaa ggggcgatta ttatcttcgc cgtagcgctg gattcactga
    28201 aatacgcgcg taagaagtga tgtgattgcg gatattgccg gatgcgtctc tggttttaca
    28261 acgttaccgg gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac
    28321 ccaccgggca gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg
    28381 gtgtgcgggt tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat
    28441 aacagcgtcc acagccaaca catttgtcaa gattgatacg gggatagaca atataactgc
    28501 ggtctaaatc ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcaacg
    28561 aatcaaatcc ctgatcggcg agataatgcg ataacccgct cgccatatct tctacgatgc
    28621 gatagccgta ctgcataatg ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa
    28681 actccgccgc atcttcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca
    28741 gttctggatg ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac
    28801 cggaatatcc ggaaatactc gatttaccgt taacgatcgg cataccgatt ttctgattaa
    28861 gatcgatatt ggtgatggat ttaacggtgt taattgccgc aatgccatct gcgccgccgc
    28921 gcttcgccgc cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca
    28981 ttggcagcgt ggaaccccgt ttcactgccc gacaatattt ttttaccagc tccgggcttt
    29041 gcccgacatc gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga
    29101 tcatatccgc gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt
    29161 cccccatgat cgaagcgatc aatactttgt ccgggtaatc ttccttcagc cgacgcaggg
    29221 cggtcagatt ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa
    29281 aaccggtatc ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa
    29341 agccgatcgt tttaaacacc acaccgcccc aacctgtgtc gtaggctttg gcacacatct
    29401 catagcagtt gcctaccggc gaagaagaga ggcagaacgg gttgggaaag ttcacgccgc
    29461 aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct ccctctaata tagtgatgaa
    29521 tcgcctcggc ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa
    29581 caatatcgcc agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg
    29641 tgttacgctg ctgctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga
    29701 gaataatttt atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc ttaaacgtga
    29761 ctttattgcc ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc
    29821 gggcgctggc aaactctttt tcgcttgccg gaaactcatc taactcttca cgtgctacgc
    29881 aagttaccgc ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac
    29941 cgccgccgat aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc tgtcgtacac
    30001 gttgcaggaa gtcgacggca atctcaacgt cgctatgctc aaacagcggt agcccggaac
    30061 cgcttgataa ccccacggtg accagtaccg cgcggttttc cgctttcagt tgctccaggg
    30121 tgagtgtttt accgatttcg ttattgcact taatggtcac gcccattttt tcaatacggg
    30181 cgatctctgc atccagcact gattgcggta accggaattg cggaataccg ttacgcaacc
    30241 agccaccggg gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg
    30301 tcacactggc ctgcaatccg gcaggacctg cgccaataat cgcgactttg ccgagcgttt
    30361 tagtaccggg ctgataaatt tccatcccgg tttgttgttc aaaatcagta acaaaacgct
    30421 gtaagcggcc gatatcaatg ggagcatcaa caccggcacg ggtacaaccg ctttggcata
    30481 atttctccgt cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg
    30541 tctcggcagc gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg
    30601 tctgtgccgg gcaagcctga ctacagggag cgtcgtgaca taataaacag cgagaggctt
    30661 ctttaatcgc cagtaacggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg
    30721 gcatgtgatc ttccttaatg ctatgagtaa ttatggcgaa cgctaaataa atattgcgtg
    30781 ctgatattaa ggctatgcgc gtggggaaat ttaaactgtg aaaggattca caaatagctg
    30841 cggttcagaa tgttatatcc gcagcttatt gagtgaagta ataattattc tcaataatta
    30901 acagaaaaga gaaaatatta acgatagaaa ataagataaa caatcatgcc gatagataaa
    30961 attccgaagc caatatatgc caccatataa gcgaagaaga gtccgggatt ttttgctcgc
    31021 cagacataca ccggttccgg tgcattttta acccgtttat caatcgcgcc tgcgcgccag
    31081 cctttccatg cttcgcggaa acaaaatatc gggataagga aaaaaacggc aacgacaaaa
    31141 aactgctgta catccataac tactttcctt gtttcttttg taattcaagt aactgttcgg
    31201 gtgtaacggc gggatacccc tgcgcatctt ccggcacctg gtgcatagcc ggaataggga
    31261 ccagcggccc taaaaaacgc ggttcacgtt taaaaatata aaggtcagcc agcgcaccga
    31321 aacgggcggc aaattcgcga atacgtactg acagcatatc tttcggtgac ggtacggcat
    31381 aacactgagc ctgaatcccc atatgcagcg caataaataa tgctcgctca cagtggaaac
    31441 gttgggtgat aataatgaaa tcattagtat cgaaaacttt gcgtgtacgc acgatggaat
    31501 ccagcgtacg aaagcctgcg taatcgagaa caatatctga tgggtcgaca ccagcagcga
    31561 ttaaatcttt gcgcatggtc atcggctcgt tataactttg caatgcgtta tcgccgctca
    31621 gtaatagata atttacctta ccgctgttat aggcattaat cgctccttga atgcggtagc
    31681 gataatactg attaataacg ccggtacgat aatattttgc tgtgccgagc accacgccga
    31741 cctggcggta ggggagatcc tgcaattcgt cgtagatata aggcgcggtt ttccagctca
    31801 tccagcgatc gaggccgagc acagtcaaca gcagcaagcc gatcaggact aacaggctga
    31861 ggaacacgcg ctttaacatg caggagactc gagctaagga aaatttccat caggctactt
    31921 tacccgctgg ttttgcgcaa gaaacggcaa actgtttgcc gggaatttcg acaccaggag
    31981 agggctggca agccggacaa ggcgttcacg ccgcatccgg caccaggctt aagcgagaag
    32041 cacgcggtcg atactgtgac agccgagagc tttcagcgtt gcggaggtgg catcccactg
    32101 aatcaacggc gcatcggctt tttctgccag aaccgcgcgc tggatatccg ggtaatcata
    32161 acccttgaga ttcaacagaa ttaacgcccc ctgcaacggc ggcagagtcg ggttgaatgc
    32221 ggcgttttca gcgtagctgc cgctgaaaat acgaccgtct ttacattcca gcgcgacacc
    32281 gcttggtgac ttactgtaag gcatgtgcga acggtttgcc gctgcaatcg ccgcctgaga
    32341 aagcgcatca ccagtcagcg catagccgtg atcctgctcg tccatcagca gcgttttaat
    32401 ctccagatct ttcggcccaa aggcatccgg cagatagtca cgcagcgcgt gtgcctcgcg
    32461 gcccggcaaa tgaatacgca gatccagacc gctgttcagt tcattcataa actgacggca
    32521 gtgaccacaa ggcgtatagt taacggtgat ggctgcaagc gctttttcag cactcaacca
    32581 ggcgtggctg atcgcgcttt gttcggcatg aacggtttgc tgcattgttg caccaataaa
    32641 ttccatattg gcaccaaaat accaggttcc gctcacaccg cgcgcaattg cgccaacatt
    32701 aaaattcgac aatggcgtac gcgcacaggc cgccgccagc ggaagtagtg cgaatgccag
    32761 cgcgtcttcg tccagccccg ttgcgctctt cagcgatgag acttgctccc cggtcaacaa
    32821 agcggggaag tacttgtctg ccagaatagg ttccagtgca gattgcaagt tatccgcaag
    32881 ttgggcaaaa gcggtttgaa aacgtggatg catggcgttg cctcataatc atgtaatggg
    32941 ataacagttt acgaacccgt tatggcttta tatgtgatct gaatctcatt aattatgcaa
    33001 cacgtgaaac ttacatcaaa aatgcgcgac gtatcgcaaa ttttagccca ttactgccag
    33061 aataatcggg aaaaggaacg gtgcgatcag cgaagtaatt atcccgcata acaccagcgc
    33121 tagcgaacta aatgcacctt cctgataatc cagctcggcg cagcgcgccg taccgagggc
    33181 gtgcgaggca gtccccattg ccagaccgcg cgcagctttg gtacgaatac gcatcgtatt
    33241 aagcaatgta tggccaaata ccgcgccgag gatgccgacg aaaatcacgc aaactgcgct
    33301 gattgccgga ataccgccaa tactgccgcc aaccgccatt gcaataggcg tggtgactga
    33361 tttcggcagg attgacgcgg cgatttccgg tgaagcgccc atcaacaatg ccacggaagt
    33421 ccccgtcacc atcgcaacca cgctgccgat aaaacagatg gtgatgatcg atttccagcg
    33481 cgcgcggatc tggtgtagct gctcatataa aggataggcc agcgcgacga ccgccggttg
    33541 cagcagatcg tttagcactt cgctaccttt aaagtaactg tcgtaagaga taccggtcag
    33601 cattaaaaaa ggaatgatca ccaccattgc taccagcaac gggttaagca ggggaaactt
    33661 ataccgtgcc gccagtttgc gggcggcaaa aaagacaatc aacgtcagcg gtaatgacca
    33721 ccagatattc gccatcattc ttctgatcct ttctgaccta cgactttacg ttcaccgtgt
    33781 accagttgtg aactccagct caccaccaga aaaaccacca gcgtactgac agcacaggaa
    33841 accactaccg ggccaaactg cgcgcggagc aaatcaaaat attgcatgac gcctacgcca
    33901 atcggcacaa acaatagcgc catatagcga atcagtacgt agcaccccgg attgacccat
    33961 ttcgccggaa gaatttgcaa ggccagcagg acaaacagga tcagcatccc gatgatgctg
    34021 cccggaatgg ttaccggtag cagggaagca atgaaaatgc ctgcatacag gcaggcataa
    34081 atcaggacga aagcgcgtaa atattgccag ataatgttca gtgtcttact cataatcaac
    34141 accccagatg agatggattc atcatacaat taattcgtaa aacgtgctac cgatcacatt
    34201 aagaaatcag cgcttatttt ttcgctaccc ggtattacgc aatgagcagc gaactcaggc
    34261 atacatcaag catagagagg gattgcagtt tgcagcaatc cctctttgca gacctttaat
    34321 ccgctgacca taggcgctaa catagcgcct catttttttg cgggtgatga tatacgtgtg
    34381 ttactggcac cgatggaggg agtgcttgac tctctggtgc gtgaattgct gaccgaagtt
    34441 aacgactacg atctgtgcat caccgagttt gtccgcgtgg tggatcaact gctgccggta
    34501 aaagtctttc atcgcatttg ccctgagcta caaaacgcca gccggacacc atctggtacg
    34561 ctggtgcgcg tgcagttgtt aggtcagttc ccacaatggc tggcagagaa cgccgcccgt
    34621 gcggtggagt taggttcctg gggcgtggat ctcaattgcg gctgcccgtc gaaaacggtt
    34681 aacggtagcg gcggcggggc gacgttactc aaagatcctg aactcatcta ccagggggca
    34741 aaagcgatgc gtgaagctgt accggcgcat ttgcctgtca gcgtgaaagt gcgtctgggc
    34801 tgggacagcg gtgagaagaa atttgaaatc gccgatgcgg ttcaacaggc tggcgctacg
    34861 gagctggtgg tgcatgggcg gacgaaagag cagggttacc gcgcggagca tattgactgg
    34921 caggcgattg gcgagattcg ccagcggctg aatattccgg tgattgccaa cggtgaaatc
    34981 tgggactggc agagcgcgca acaatgcatg gcgatcagcg gctgcgacgc agtgatgatt
    35041 ggtcgcgggg cgctcaatat tcccaacctg agccgggtgg taaaatataa cgaaccgcga
    35101 atgccgtggc cggaggtggt tgctttgctg caaaaatata cccgtctgga aaagcagggc
    35161 gataccgggt tatatcacgt tgcgcggatt aaacagtggt tgagttattt gcgtaaagaa
    35221 tacgatgaag caacggaatt atttcagcat gttcg
//
LOCUS       DADMFL010000031        35102 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.031, whole genome shotgun sequence.
ACCESSION   DADMFL010000031 DADMFL010000000
VERSION     DADMFL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.031"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            617..2902
                     /gene="nrdA"
                     /locus_tag="JNZ27_001508"
     CDS             617..2902
                     /gene="nrdA"
                     /locus_tag="JNZ27_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY8663889.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3136..4266
                     /gene="nrdB"
                     /locus_tag="JNZ27_001509"
     CDS             3136..4266
                     /gene="nrdB"
                     /locus_tag="JNZ27_001509"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY8663890.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4266..4520
                     /gene="yfaE"
                     /locus_tag="JNZ27_001510"
     CDS             4266..4520
                     /gene="yfaE"
                     /locus_tag="JNZ27_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY8663891.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4574..5224)
                     /gene="inaA"
                     /locus_tag="JNZ27_001511"
     CDS             complement(4574..5224)
                     /gene="inaA"
                     /locus_tag="JNZ27_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY8663892.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5388..5630
                     /locus_tag="JNZ27_001512"
                     /pseudo
     CDS             5388..5630
                     /locus_tag="JNZ27_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5672..6748)
                     /gene="glpQ"
                     /locus_tag="JNZ27_001513"
     CDS             complement(5672..6748)
                     /gene="glpQ"
                     /locus_tag="JNZ27_001513"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8663893.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6753..8111)
                     /gene="glpT"
                     /locus_tag="JNZ27_001514"
     CDS             complement(6753..8111)
                     /gene="glpT"
                     /locus_tag="JNZ27_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY8663894.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8384..10012
                     /gene="glpA"
                     /locus_tag="JNZ27_001515"
     CDS             8384..10012
                     /gene="glpA"
                     /locus_tag="JNZ27_001515"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY8663895.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10002..11261
                     /gene="glpB"
                     /locus_tag="JNZ27_001516"
     CDS             10002..11261
                     /gene="glpB"
                     /locus_tag="JNZ27_001516"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY8663896.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11258..12448
                     /gene="glpC"
                     /locus_tag="JNZ27_001517"
     CDS             11258..12448
                     /gene="glpC"
                     /locus_tag="JNZ27_001517"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY8663897.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12641..13591
                     /locus_tag="JNZ27_001518"
     CDS             12641..13591
                     /locus_tag="JNZ27_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8663898.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13632..14102)
                     /locus_tag="JNZ27_001519"
     CDS             complement(13632..14102)
                     /locus_tag="JNZ27_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8663899.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVSVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14157..15359)
                     /locus_tag="JNZ27_001520"
     CDS             complement(14157..15359)
                     /locus_tag="JNZ27_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY8663900.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15446..15988)
                     /locus_tag="JNZ27_001521"
     CDS             complement(15446..15988)
                     /locus_tag="JNZ27_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663901.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16267..16692
                     /gene="nudI"
                     /locus_tag="JNZ27_001522"
     CDS             16267..16692
                     /gene="nudI"
                     /locus_tag="JNZ27_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY8663902.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16731..17333)
                     /locus_tag="JNZ27_001523"
     CDS             complement(16731..17333)
                     /locus_tag="JNZ27_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY8663903.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17641..18780
                     /gene="arnB"
                     /locus_tag="JNZ27_001524"
     CDS             17641..18780
                     /gene="arnB"
                     /locus_tag="JNZ27_001524"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY8663904.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18784..19752
                     /gene="arnC"
                     /locus_tag="JNZ27_001525"
     CDS             18784..19752
                     /gene="arnC"
                     /locus_tag="JNZ27_001525"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY8663905.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTGLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19752..21743
                     /gene="arnA"
                     /locus_tag="JNZ27_001526"
     CDS             19752..21743
                     /gene="arnA"
                     /locus_tag="JNZ27_001526"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY8663906.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPSCPK"
     gene            21731..22621
                     /locus_tag="JNZ27_001527"
     CDS             21731..22621
                     /locus_tag="JNZ27_001527"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY8663907.1"
                     /translation="MPKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22621..24273
                     /gene="arnT"
                     /locus_tag="JNZ27_001528"
                     /pseudo
     CDS             22621..24273
                     /gene="arnT"
                     /locus_tag="JNZ27_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24270..24605
                     /gene="arnE"
                     /locus_tag="JNZ27_001529"
     CDS             24270..24605
                     /gene="arnE"
                     /locus_tag="JNZ27_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY8663908.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24605..24991
                     /gene="arnF"
                     /locus_tag="JNZ27_001530"
     CDS             24605..24991
                     /gene="arnF"
                     /locus_tag="JNZ27_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY8663909.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24985..25257)
                     /gene="pmrD"
                     /locus_tag="JNZ27_001531"
     CDS             complement(24985..25257)
                     /gene="pmrD"
                     /locus_tag="JNZ27_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY8663910.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25362..26717)
                     /gene="menE"
                     /locus_tag="JNZ27_001532"
     CDS             complement(25362..26717)
                     /gene="menE"
                     /locus_tag="JNZ27_001532"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY8663911.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26714..27676)
                     /gene="menC"
                     /locus_tag="JNZ27_001533"
     CDS             complement(26714..27676)
                     /gene="menC"
                     /locus_tag="JNZ27_001533"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY8663912.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27676..28533)
                     /gene="menB"
                     /locus_tag="JNZ27_001534"
     CDS             complement(27676..28533)
                     /gene="menB"
                     /locus_tag="JNZ27_001534"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY8663913.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28548..29306)
                     /gene="menH"
                     /locus_tag="JNZ27_001535"
     CDS             complement(28548..29306)
                     /gene="menH"
                     /locus_tag="JNZ27_001535"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY8663914.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29303..30973)
                     /gene="menD"
                     /locus_tag="JNZ27_001536"
     CDS             complement(29303..30973)
                     /gene="menD"
                     /locus_tag="JNZ27_001536"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY8663915.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31062..32357)
                     /gene="menF"
                     /locus_tag="JNZ27_001537"
     CDS             complement(31062..32357)
                     /gene="menF"
                     /locus_tag="JNZ27_001537"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY8663916.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32436..32741)
                     /gene="elaB"
                     /locus_tag="JNZ27_001538"
     CDS             complement(32436..32741)
                     /gene="elaB"
                     /locus_tag="JNZ27_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY8663917.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32796..33257)
                     /locus_tag="JNZ27_001539"
     CDS             complement(32796..33257)
                     /locus_tag="JNZ27_001539"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8663918.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33322..34239
                     /gene="rbn"
                     /locus_tag="JNZ27_001540"
     CDS             33322..34239
                     /gene="rbn"
                     /locus_tag="JNZ27_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY8663919.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34430..>35102
                     /locus_tag="JNZ27_001541"
     CDS             34430..>35102
                     /locus_tag="JNZ27_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663920.1"
                     /translation="MVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLILYNLAHTATNS
                     HLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGTN
                     FWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDPS
                     WPNEVQ"
BASE COUNT         8618 a         9394 c         8663 g         8427 t
ORIGIN      
        1 attacccccg atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata
       61 attcaagtta ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat
      121 aattttctta taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat
      181 agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact
      241 gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa
      301 gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga
      361 acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc
      421 taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa
      481 gagggtcatt ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt
      541 ctaagcagct tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa
      601 aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga
      661 gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa
      721 cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac
      781 ctctgacatc cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc
      841 ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg
      901 ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata
      961 cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat
     1021 cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata
     1081 tctggtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct
     1141 tgttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg
     1201 tttttacgac gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt
     1261 gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga
     1321 ttccatcaac gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg
     1381 catcaacgcc ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca
     1441 taccggctgt atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg
     1501 cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag
     1561 cctgctggtg ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta
     1621 cggggtacaa atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct
     1681 gttcagcccg tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt
     1741 tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc
     1801 cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca
     1861 gaacgttgac cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca
     1921 gtctaacctg tgcctggaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga
     1981 gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct
     2041 ggatgaactg gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta
     2101 tcaggattac ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat
     2161 tggtgtgatt aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag
     2221 cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc
     2281 taatgagctg gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa
     2341 agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct
     2401 gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac
     2461 gctttctgcc ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat
     2521 tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt
     2581 ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa
     2641 cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc
     2701 taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct
     2761 gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg
     2821 tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga
     2881 aagcggcgca tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg
     2941 cctacggctc ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc
     3001 cggatgcagc gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg
     3061 cgccagcgtc gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc
     3121 caacaggaca cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa
     3181 gaaccgatgt tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac
     3241 atcttcgaaa agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac
     3301 gtctcccgcg accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc
     3361 agcaacctga aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg
     3421 ctattgccgc ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca
     3481 gaaacgattc attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct
     3541 gttgtgtttg acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc
     3601 agctattacg atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc
     3661 cacaccgtta acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat
     3721 ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt
     3781 tcttttgcct ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt
     3841 gcccgcgatg aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc
     3901 ggcgcggatg atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac
     3961 ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt
     4021 tcgatgattg gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc
     4081 cgcatgcagg cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg
     4141 atcaacacct ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt
     4201 tcttatctgg tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag
     4261 ctctgatggc ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac
     4321 acccttccct tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg
     4381 aaggttactg cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg
     4441 aaccgttagc ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg
     4501 atattgaaat cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc
     4561 tggcctacaa cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag
     4621 ggatcggctc cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac
     4681 gcaagcggcg acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt
     4741 tcacatagat atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct
     4801 taaacgccag tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata
     4861 ctgcatgccg ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca
     4921 gcaacgcgcg ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga
     4981 tgacaccggc ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc
     5041 cgaacggata acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt
     5101 tgccgttgcg ctcgacgcat tgcaccctgc tcataccatt acggcgatag ttaggctctt
     5161 caacccagtc gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg
     5221 ccatacccta tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga
     5281 aaatcttaat gaaacgtgtc gtattccgct acaggtgtga caccgctttt gcccttcatt
     5341 tgcagcgcat agattccacc agccactacc actaatagtg cgcccagcag ataaaatgtg
     5401 aatttaatca gtcggataca gcgatgaatt ttattcgaca gggattaggc atcgccttac
     5461 aaccagagtt aacgctgaaa agcattgcag gtgaattgtg ttccgttcct cacgaaccaa
     5521 ccttctatcg acagatttcg ttgctgacta aagaaaagcc ggtagaaggc agtccactgt
     5581 ttttactaca aatgtgcatg gaacaattag tggcgattgg aaaaatttga tatgagacag
     5641 gatggcgcat gaacgccatc ctgcagtaac attactcttt attaagaaat tttactgcct
     5701 tatcagggaa atcagtaaac agcccattta cacccgcttt gttatacaga gcatcatata
     5761 actgattcac atcaggagtg tattcaggca gtttatctga ccgcacggta taaggatgca
     5821 ctaccagttt attctgctga gcatcttgca ccatgccagt gagtttgata ttacccggct
     5881 gcgatgtctc ctcaatcaac atatggtaat ccggaccaat accatctgca tattccgcca
     5941 cctgtttcat ggcacccggc ttaaacatcc agtcgtagtt gtaattaacc cagcttccat
     6001 ccggctgttt ctgctgcgtt tcattccagt cggtataggc aatcagctgt accagattga
     6061 gctccatgcc cattttgggt tccagctcat tcttaatacg cttcagctca tcagcatcaa
     6121 aacattgcaa ataaacttta tcgtctttac cggtgtaacc atatttcttc agcacttcca
     6181 gcgtttttgc cgcaatatcc ttcccttcct gatgatggaa ccacggcgct ttgatttctg
     6241 gatagatacc gatatttttc ccggtagagt gatttaaccc ctgaacaaat tcaatctctt
     6301 cttcaaaggt gtgcacccgg aagtcggact tacccattgg gaaacgcccc ggataagtct
     6361 gtactttttt accgttttca atatcgaaac cttcggtaaa tttcagcgac ttaatttcat
     6421 ccagcgtgaa atctatcgcg tagtaacgac cgtctttgcg cgcccgatcc gggaaacgat
     6481 cggcaacatc agtaacacga tcgagataat ggtcatgcag aacaaccaga tggtcgtctt
     6541 tggtcatcac caaatcctgt tccagataat ccgctccctg cgcatacgcc atcgcttttg
     6601 ctggcagcgt atgctccggc aaatatccac tggcaccgcg atgggcgatg actatttttt
     6661 cgttgctgtc cgccgccatt gcactgcttc ccatgactat agtgctcatc atgatcgcca
     6721 tgctaaggtt tttcagcttc aatttcatgc cattagcctc cgttgcgttt ttgcagtaat
     6781 tgttcatggc gacgtttttc gccaatcatc acaacaatca acaagataac cgccagaatg
     6841 ctgccgccaa tcattaccat aaagccgcca tcccagccga agaagtccac ggtgtagcca
     6901 acaatcgcgc tcgccgccac cgaaccgccc aggtaaccaa acagcccggt aaagcccgct
     6961 gccgtacctg ccgctttttt cggtgccagt tccagcgcat gcagaccgat cagcatcaca
     7021 ggaccgtaga tcaggaagcc gataacaatc atacaaatca tatcgacggt tgggttacct
     7081 gccgggttca tccagtaaac gatagtcgcg atggtcacca gtgtcataaa gaagacgccg
     7141 gttgccccac ggttaccacg gaagactttg tccgacatcc agccgcacag cagggtgccc
     7201 ggaatacccg catactcata gaagaagtag gcccaggagg atttatccag cgcgaaatgc
     7261 ttaacctctt tcagataagt cggtgaccag tcgaggatgc cgtaacgcag cagataaacg
     7321 aacacgttgg cgatggcgat gtaccacagc agtttgttcg gcagcacgta ctgcatgaag
     7381 atttgcttcg cggtcagctc ctgttccgct ttttcgttat agtcgtccgg ataatcattt
     7441 ttgtactctt cgatcggcgg caagccacag gattgcgggg tatcgcgcat catcgcaaag
     7501 gcgaataatg ccaccagaat ggcgcagaaa gcaggcatat agagcgccgc atgccagtca
     7561 ttaaaccagg ccatccccag caggaacagc agcggcggaa taccaccacc gacgttgtgc
     7621 gcacagttcc acactgacac aatgccgcca cgttctttct gcgaccacca gtgcaccata
     7681 gtacgaccac acggcggcca ccccatcccc tggaaccaac cgcagaggaa caacagtaca
     7741 aacatcaccg caatgctcga cgtcgcccat ggcacaaagc ccataaacaa catcactgcc
     7801 gccgccagaa tcaaacctgc gggcaggaaa acgcgcggat tcgagcgatc cgataccgaa
     7861 cccatgatga atttcgaaaa tccataagca atcgagatcc ccgaaagggc aaaacctaaa
     7921 tcaccgcgtg agaatccctg ctcaaccaga taaggcatag caagcgcaaa gttcttacga
     7981 accaaatagt aagccgcata gccaaagaat atccccagga aaatttgcca gcgcaatcga
     8041 cgataagtcg gatcgatctc cgcggcaggt aagcgcgctt tgtgtggcgc tggtttaaaa
     8101 atactcaaca ttgatagcct ccgtggcccg tggtcttatt tatgattaac agcctgattc
     8161 agtgagagaa cctgccgttt cttgagttgc cgcgatgtta agaaaacatt cataaattaa
     8221 atgtgaattg ccgcacacat tattaaataa gatttacaaa atgttcaaaa tgacgcatga
     8281 aatcacgttt cactttcgaa ttatgagcga atatgcgcga aatcaaacaa ttcatgtttt
     8341 tactatggct aaatggtaaa aaacgaactt cagagggata acaatgaaaa ctcgcgactc
     8401 gcaatcaagt gacgtgatta tcattggcgg cggcgcaacg ggagccggga ttgcccgcga
     8461 ctgtgccctg cgcgggctgc gcgtgatttt ggttgagcgc cacgacatcg caaccggtgc
     8521 caccgggcgt aaccacggcc tgctgcacag cggtgcgcgc tatgcagtaa ccgatgcgga
     8581 atcggcccgc gaatgcatta gtgaaaacca gatcctgaaa cgcattgcac atcactgcgt
     8641 tgaaccaacc aacggcctgt ttatcaccct gccggaagat gacctctcat tccaggccac
     8701 ttttattcgc gcctgcgaag aagcagggat cagcgcagaa gctatagacc cgcagcaagc
     8761 gcgcattatc gaacctgccg ttaacccggc actgattggc gcggtgaaag ttccggatgg
     8821 caccgttgat ccatttcgtc tgaccgcagc aaacatgctg gatgccaaag aacacggtgc
     8881 cgttatcctt accgctcatg aagtcacggg gctgattcgt gaaggcgcga cggtgtgcgg
     8941 tgttcgtgta cgtaaccatc tcaccggcga aactcaggcc cttcatgcac ctgtcgtggt
     9001 taatgccgct gggatctggg ggcaacacat tgccgaatat gccgatctgc gcattcgcat
     9061 gttcccggcg aaaggatcgc tgctgatcat ggatcaccgc attaaccagc atgtgatcaa
     9121 ccgctgccgt aaaccttccg acgccgatat tctggtgcct ggcgatacca tttcgctgat
     9181 tggtaccacc tctttacgta ttgattacaa cgagattgac gataatcgag tgacggcaga
     9241 agaggttgat attctgctgc gtgaagggga aaaactggcc cccgtgatgg cgaaaacgcg
     9301 cattttgcgg gcctattctg gcgtgcgccc gctggttgcc agcgatgacg acccgagcgg
     9361 acgtaacgtc agccgtggca tcgtgctgct cgaccatgct gaacgcgatg gtctggacgg
     9421 atttatcacc atcaccggtg gcaaactgat gacctatcgg ctgatggctg aatgggctac
     9481 cgacgcggta tgccgcaaac tgggcaacac gcgcccctgt acgactgccg atctggcact
     9541 gcctggttca caagaacccg ctgaagttac cttgcgtaaa gtcatctccc tgcctgcccc
     9601 gctgcgcggt tctgcggttt atcgtcatgg cgatcgcacg cctgcctggc tgagcgaagg
     9661 ccgtctacac cgtagcctgg tatgtgagtg cgaagcggta actgcgggtg aagtgcagta
     9721 cgcggtagaa aatttaaacg ttaatagcct gctggattta cgccgtcgta cccgtgtggg
     9781 gatgggcacc tgccagggcg aactctgcgc ctgccgcgct gccggactgc tgcaacggtt
     9841 taacgtcacg acgtccgcgc aatctatcga gcaactttcc accttcctta acgaacgctg
     9901 gaaaggcgtg caacccatcg cctggggaga cgcactgcgc gaaagcgaat ttacccgctg
     9961 ggtttatcag ggactgtgtg gtctggagaa ggagcagaaa gatgcgcttt gatactgtca
    10021 ttatgggcgg cggcctcgcc ggattactct gtggcctgca actgcaaaaa cacggcctgc
    10081 gctgtgccat tgtcacgcgt ggtcaaagcg cgttgcattt ctcatccggt tcgctggatt
    10141 tgctgagcca tctgccagat ggtcaacccg tggcagacat tcacagtgga ctggaatctt
    10201 tgcgtcagca ggcacctgca catccttact ctcttctcgg gccacaacgc gtgctcgatc
    10261 tcgcttgcca ggcgcaggca ttaatcgctg aaagcggtgc gcaattgcag ggcagcgtag
    10321 aactggctca ccagcggatt acgccgctcg gcactttgcg tgcaacctgg ttaagttcgc
    10381 cagaagttcc cgtctggccg ctacccgcga agaagatatg tgtagtgggg ataagcgggc
    10441 tgatggattt ccaggcgcat cttgcggcgg cttcgttgcg cgaactggat ctttcggttg
    10501 aaaccgcaga aatagagctg ccggaactgg atgtcctgcg caataacgcc accgaatttc
    10561 gcgcggtgaa tatcgcccgt ttccttgata atgaagaaaa ctggccgctg ttacttgatg
    10621 cgcttattcc tgtcgccaat acctgcgaaa tgatcctgat gcccgcctgc ttcggcctag
    10681 ccgatgacaa actgtggcgt tggttgaatg aaaaactacc ttgttcactg atgcttttgc
    10741 caacgctgcc gccttccgtg ctgggcattc gtctgcaaaa ccagttacag cgccagtttg
    10801 tgcgccaggg tggagtgtgg atgccgggcg atgaagtgaa aaaagtgacc tgtaaaaatg
    10861 gcgtagtgaa tgaaatctgg acccgcaatc acgccgatat tccgctacgt ccacgtttcg
    10921 cggttctcgc cagcggcagt ttctttagtg gcggactggt agcggaacgt aacggcattc
    10981 gcgagccgat tctcggcctt gatgtgctac aaaccgccac gcggggtgaa tggtataagg
    11041 gcgatttttt tgcgccgcaa ccgtggcagc agttcggtgt aaccactgat gagacgctac
    11101 gcccgtcgca ggcagggcaa accattgaga acctgtttgc catcggttcg gtgctgggcg
    11161 gatttgatcc catcgcccag ggatgcggcg gcggtgtttg tgccgtcagt gctttacatg
    11221 ccgctcaaca gattacccaa cgcgcaggag gccaacaatg aacgacacca gcttcgaaaa
    11281 ctgcattaag tgcaccgtct gcaccaccgc ctgcccggtg agccgggtga atcccggtta
    11341 tccagggcca aaacaagccg ggccggatgg cgagcgtctg cgtttgaaag atggcgcact
    11401 gtatgacgag gcgctgaaat attgcatcaa ctgcaaacgt tgtgaagtcg cctgcccgtc
    11461 cgatgtgaag attggcgata ttatccagcg cgcgcgggcg aaatatgaca ccacgcgccc
    11521 gtcgctgcgt aattttgtgt tgagtcatac cgacctgatg ggtagcgttt ccacgccgtt
    11581 cgcaccaatc gtcaacaccg ctacctcgct gaaaccggtg cggcagctgc ttgatgcggc
    11641 gttaaaaatc gatcatcgtc gcacgctacc gaaatactcc ttcggcacgt tccgtcgctg
    11701 gtatcgcagc atagcggctc agcaagcaca atataaagac caggtcgctt tctttcacgg
    11761 ctgcttcgtt aactacaacc atccgcagtt aggtaaagat ttaattaaag tgctcaacgc
    11821 aatgggtacc ggtgtacaac tgctcagcaa agaaaaatgc tgcggcgtac cgctaatcgc
    11881 caacggcttt accgataaag cacgcaaaca ggcaattacg aatgtagagt cgatccgcga
    11941 agctgtggga gtaaaaggca ttccggtgat tgccacctcc tcaacctgta catttgccct
    12001 gcgcgacgaa tacccggaag tgctgaatgt cgacaacaaa ggcttgcgcg atcatatcga
    12061 actggcaacc cgctggctgt ggcgcaagct ggacgaaggc aaaacgttac cgctgaaacc
    12121 gctgccgctg aaagtggttt atcacactcc gtgccatatg gaaaaaatgg gctggacgct
    12181 ctacaccctg gagctgttgc gtaacatccc ggggcttgag ttaacggtgc tggattccca
    12241 gtgctgcggt attgcgggta cttacggttt taaaaaagag aactacccca cctcacaagc
    12301 catcggcgca ccactgttcc gccagataga agaaagcggc gcagatctgg tggtcaccga
    12361 ctgcgaaacc tgtaaatggc agattgagat gtccacaagt cttcgctgcg aacatccgat
    12421 tacgctactg gcccaggcgc tggcttaaac tcccttctga tgcccggtaa gcatgtggtt
    12481 accgggcatt tttgcgtaca cgattccgtg cccaatgtat gcgttgcaac gcagtgaaaa
    12541 ttcctctgaa aacgtctcgc aaaggctgaa actggcagat gtcaaaggcc tgagataacc
    12601 gtaatgtcgc gtcatcataa atatcaggtg acggacaacc atgaccgaat caacaacctc
    12661 ctccccgcat gatgcggtat ttaaaacctt tatattcaca cccgaaaccg cacgggattt
    12721 tctcgaaata catttaccag aaccactgcg caagctttgc aacctgcaaa ccttacgcct
    12781 ggaacccact agttttattg aaaaaagttt acgcgcttac tactcggatg ttttgtggtc
    12841 cgtggaaacc agcgacggtg acggctatat ctactgcgtg attgaacatc aaagctctgc
    12901 agaaaagaat atggcttttc ggccaatgcg ctatgccact gccgccatgc agcgtcacct
    12961 agataaaggc tatgacagag ttccgctggt ggtgccattg ctgttttatc atggcgaaac
    13021 atcgccctac ccgtactcac ttaactggct ggatgagtct gacgatccgc aacttgcccg
    13081 gcagttgtac accgaagctt ttccgttggt ggatatcacc atcgtacctg acgatgagat
    13141 catgcaacat cggcgtatag ctctgctgga actgattcaa aagcatattc gcgaccacga
    13201 tttaatcggc atggtcgaca ggatcaccac gcttttggtt agaggcttca ctaatgacag
    13261 ccagctacaa acactgttta attatctgct gcaatgcggc gatacctccc gtttcacccg
    13321 ttttattcag gagattgccg aacgttcacc actacaaaag gagagattaa tgactattgc
    13381 tgaacggcta cggcaggaag gacatcaaat tggctggcaa gaaggtaaat tagtaggttt
    13441 gcagcaaggt aaattagaag gtttgcagga aggtatgcat gaacaagcca ttaaaattgc
    13501 tttgcgcatg ctggaacagg gcattgatcg tgaccaagtg ctcgcggcca cccagctaag
    13561 cgaagccgat ctggcagcga ataaccacta attaacacag gcccacagcc gatccccatg
    13621 ggcctttgat atcaataact accttttatg cgtggcccat ttttgcctga tttaaacatc
    13681 gccagtcgtt gatccagggc atcgctgtag agcatcgtgt caacgctaac agcgacaaag
    13741 ttcgctcccc acgccaggca ttgctgcgcc atatcaggag ccacagccag aaaaccagcc
    13801 gctttacccg cagcacggat ccgccgaatg ctggtttcaa taattcgctg cacttccggg
    13861 tgcccggcgt tatccgggta gcccaacgac gcagaaagat ccgctggtcc aataaacacg
    13921 ccatcaatcc cttcgacgtc gaggatctcg tccaggttat ccagtgccgt tttactttcc
    13981 acctgcacta acagacaaag cgaatcgttg acttgcgcca tgtaattctc aatgcgtccc
    14041 cagcgcgcag cccgtgccac actggcccca acaccacgct caccgtaggg agggttggac
    14101 aaattttgcc ggagggggaa tgtgccggtg cagtaaaccg gcacagagtg gcgaaatcag
    14161 acggataaag tcatggactc aacaacctca atccagccat gctcactagc gatgtcctgg
    14221 ccgtttaacc aacggcgtag catattcagt gccatcattg cgcacacttc ctgacggata
    14281 gccaggctgt agcgtgtagt gctgaaacgc acacgcagag cgaaagtgcc gtctggcgtg
    14341 gctagcgcaa agttgagatg ctcgttctcg aaacccgaaa cagccagtgc cagcccggca
    14401 aaatggttgg cccgccgttc tgtaatccag tgcgcagttt gcgccagggt ttcttcctgt
    14461 gaaggaatca cttcacacgc cagcaatgga gcacctgcgc gagaaagttg caaagccaat
    14521 aaaccaccgg tgaattgctc gctcaacgtc aggctgaact ggcggttttg caattcgcga
    14581 ctgatctgcg cgggcagtcc ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt
    14641 ttaacgtcca gccacagttt ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc
    14701 agttcgatga tcggcattga ggagcgatag cccattgtta cgcccggcgg cagttgtaga
    14761 gtgtccaggc tttgtgccag atcgctttcc gaacgaccaa aggtagttaa acgcagacaa
    14821 accggcggct gtggtaaaga aaaacgctcg cgcaggcgcg gcaggatttc gtgctcgacc
    14881 atcaccttaa attctgacgg tacgccggga gtaaagaaca tcaggcaacg attaagctgc
    14941 acggcaaaac cacaggcggt acctaccggg ttattgataa attcagcact ggcaggcagc
    15001 tccgcttgtt tacggttgct cggtgccatt actcgtccac gttcgtggaa atagcgttcc
    15061 atctctttga gccaggcttc atgcagcaca aggccttcac cttttgctgt cgcagcggcg
    15121 agtgcgctta aatcatcgct ggtcggtccc agcccgccgt taacgatcag cacatcggcg
    15181 tgctgactac gttcgcgaag aatggtgact aagtcatcaa ggttatcccc caccgtattg
    15241 cggcgagata atggcagccc ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg
    15301 tcaacgattt gcccgtataa cacttcatcc ccggtggata gcatttccac ttttaacatg
    15361 gttatctcct gcttttgtta tcgaatcact ataacgcaaa gggagataag gcgtagtaag
    15421 aaactgaatg ccgcgccgga agggatcaga aactggcgtt aaccccaaca tacaggccgt
    15481 cagccacagc gttgtcgcgg ttaccgtctt tacccgacag attcaggtag cgataacccg
    15541 cctcaatact gactggacgc ataatggtgt aacgcgcgcc agcattcgct tcctcataac
    15601 tttgaatacc gctggagagc gaatccggag agtagtaata ctcgccaaac aaacggaagc
    15661 tgttgccaat tttccactgc aaaccacctc ctaccgctgc ggcataacct tcatcgccgt
    15721 aattcgggtt ggtgtacacg ccttttccgc caacggtcgc cattaacggc ccgagaggca
    15781 gattcaaccc cagccccacg cccgcgacgt caccgtcgtc gtcgttatgt gtccagttac
    15841 cgcttaaagc caggcccgtc gattcagtcc caaaaccgac accaatattg gtgtattctt
    15901 tacccgcctg gccgctgatg ctcattgcat tgaccgatgc agaaaccaac agcattccgg
    15961 ccagacctgc tagcgcaatt tttttcatta tcccctttcc cacaagcaat taatcaaaat
    16021 tcccaaagct gcgggattgt actgcctggc acagaggatt caatgagcga aaaaagggat
    16081 ctagtcactt attgtaacta aatacttctt aaataattaa acgccgaaaa aataaacaca
    16141 ctttaccgat acacacaaaa ataataataa gtgcagttat cattgcaaaa gagattactg
    16201 tcactttcac atatttctga gttaccgtat tctcatggtt tctttttatt tataaggaat
    16261 acccctgtgc gacaacggac tattgtatgc cctttgattc aaaatgatgg tgcttatttg
    16321 ctgtgcaaaa tggccgacga tcgcggcgtt ttccccggtc aatgggcgct ttcgggtggc
    16381 ggcgtggagt ctggcgaacg aattgaagag gcactacgcc gcgaaattcg cgaagaactg
    16441 ggagaacagc tgcttttgac agaaatcacg ccgtggacct tcagcgatga tattcgcacc
    16501 aagacatatg cagatggtcg caaggaagag atttatatga tttacctgat ttttgactgc
    16561 gtttctgcca accgagaagt gaaaataaac gaagagtttc aggactacgc gtgggtaaaa
    16621 cctgaagatc tggtgcatta tgatttaaat gttgccaccc gaaaaacgtt acgtttgaaa
    16681 ggtcttcttt aacaatgatg acagccatca ctggctgtca gattgaaaat ttagtggtta
    16741 acgaattccc catccagata aactttgcct ttttccacat gcatgactaa accatccaga
    16801 taatcaggtt taaatatcgc atcacgctta tttttagcaa tatatgtcag gcaatgatta
    16861 tgggtgaaaa taacgatatt tttatcaggc gctttgcttt gtaagtcctt aattgcacta
    16921 taaatctcat taccgcactg aagaagtcgt ttatctaccg tcagcttttt acccgcggaa
    16981 aaccaggtcg ccgactgaat ggtccggacg gtattactgg aataaagatc gaaatcaggg
    17041 atgtcagcgc taaaagcgtt gcccagttca cgggcatcct gggtaccttt aaccgtaata
    17101 cctgttttat ctgacaagca ttgattggtt gaacgatcgc aacgttcagc atggcgaaac
    17161 aaaacgacaa ccgggtgctg ctgtgccagt ctggccaatg ttttattgtc gatacgtggc
    17221 aaaccattgc tactccaggc ggcatgagta cccagtccgg caattgcagc gagcgccagt
    17281 aaaatgatga aatatttttt tgacttcaat gatgagcggc aaaaagctaa cacgtaaact
    17341 ccacctgtag acaagcgcaa ccagacaatt accgtgaaat tgagcgacat ttctggcgat
    17401 aattagcagt tggtgtaata ttaaaaatcc tacgatgtcg gcaaaatgcc tcaaattttt
    17461 gccaaatgca aagcctaaat aagaaaaaat ataaaaattt caatatttac gtctaatatt
    17521 agtttcttaa ggttaagtta atattctatc cttaaaattt cgctccaaat ggcaaaatat
    17581 acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga aggaaaagca
    17641 atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact cgctgcagtt
    17701 aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgagcaa
    17761 gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga
    17821 atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc
    17881 ctgacctggg tttcaaccct caatatgatt tccttgctgg gtgcaacgcc ggtaatggtg
    17941 gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgaatcagc cattacgcca
    18001 cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacaccatt
    18061 cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt
    18121 acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc atttcatgcc
    18181 attaaaaata ttacctgtgc tgaaggtggc ctgattgtaa ctgataatga aaaccttgcc
    18241 cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacaaacc
    18301 tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa tctgaccgat
    18361 attaacgccg cgattgccct gacacagtta gtcaaattag agcacctcaa cacccgtcgg
    18421 cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc
    18481 cttcccgcct ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa
    18541 cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg cattggtacc
    18601 gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg
    18661 tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt cgttgccgct gttcccggat
    18721 atgactaccg ccgatgccga ccgcgtcatc acagcccttc agcaactcgc aggacaataa
    18781 gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag
    18841 caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc gttggggaaa
    18901 gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc
    18961 gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg caattacggg
    19021 caacattcag cgattatggc gggattcagt cacgttactg gcgacttaat tattaccctt
    19081 gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa agccgatgaa
    19141 ggttacgacg tggtagggac tgtacgccag aaccgccagg acagctggtt tcgtaaaaca
    19201 gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat gggtgactac
    19261 ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa
    19321 cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat tgaaattcca
    19381 gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg cctgattaat
    19441 ttgatgtacg acctggtgac cggccttacc acaacgccgc tacgtatgct gagtctgctc
    19501 ggcagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat tttacgcctg
    19561 accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact
    19621 tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg caggatctac
    19681 accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag
    19741 gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg
    19801 aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc gataatcccg
    19861 gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagagac attccggttt
    19921 atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa ctgtcaccag
    19981 aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttt cagctcgctc
    20041 ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt cgcgcgccgc
    20101 tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac cgaatggtaa
    20161 aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg ccagacgata
    20221 tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat
    20281 tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct
    20341 gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg gcatccgtac
    20401 tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca
    20461 atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg
    20521 ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg
    20581 tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagatgctgg
    20641 gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga cgccgtaccc
    20701 gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc
    20761 gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgttttc
    20821 tgaatcatcc acattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg
    20881 agtatcacgt caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc acgccgattg
    20941 aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat ctgcgcatta
    21001 tccgctactg cgttaagtac cgtaagcgaa tcatcttccc gtcaacttca gaagtttatg
    21061 ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga
    21121 ataaaccacg ctggatttat tcggtgtcaa aacaattact tgatcgggtg atctgggcct
    21181 atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg atgggaccac
    21241 gactggataa ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc
    21301 tcaatctggt agaaggttca ccaattaagc taattgatgg cggaaaacaa aaacgctgct
    21361 ttactgatat ccgggacggt atcgaggcgt tgtaccgcat tatcgaaaat gcgggaaatc
    21421 gctgcgacgg cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaggaac
    21481 tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat ttcccaccgt
    21541 ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat caggacgtag
    21601 agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag ccgaaaattg
    21661 atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg
    21721 ataaaccatc atgcccaaag taggcttacg cattgatgtc gatacctttc gtggcacccg
    21781 tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt
    21841 tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga agccacagtt
    21901 tttgtggaag atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc
    21961 aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca ttcgtgaagc
    22021 ggctaaacat cacgaagtcg gtctgcacgc ctgggatcac catgcctggc aagcccgtag
    22081 cggtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc gcactctgga
    22141 agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg ccgaccagaa
    22201 ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggccat
    22261 gccgttccgt ccactgctcg aatcaggaaa ccctggcact gcgcaaattc cggtgacctt
    22321 acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt
    22381 actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg cagaagttga
    22441 aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc aggaaggcgt
    22501 gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac aagttgttcg
    22561 cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcgctg
    22621 atgaaatcgg tacgttacct tatcggcatc ttcgcgttta ttgcctgcta ttacctgtta
    22681 ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga aatcagtcga
    22741 gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg ttatttcgaa
    22801 aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac
    22861 tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg
    22921 tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt aatttatctc
    22981 tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgcattc
    23041 tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc
    23101 aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt
    23161 tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac gcaaaaacgc
    23221 tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc
    23281 ccctggggac tggcgatagc gcagcgggag cctgacttgt ggcactattt tttctgggtt
    23341 gagcatattc aacgctttgc actagatgat gcccaacata gagctccgtt ctggtactac
    23401 gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac
    23461 acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg
    23521 ccgctgctgt ttttctccgt cgctaaaggc aaattgccca cctatattct ttcttgcttt
    23581 gcacctctgg caatgctgtt ggcgcattac gctttgctgg cagcaaaaaa taatcctctg
    23641 gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca
    23701 tttgtgatct ccccctgggg accaatgaac acgccggtgt ggcaaacctt cgagagctat
    23761 aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg gtacacctta
    23821 acaaacgtcg aaaagaccta gtctttcgcc gcgctttgcc cgctggggct ggcgttgctg
    23881 gtaggatttt caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag
    23941 atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc
    24001 gcaggtctgg catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag
    24061 ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg tgatgagttc
    24121 gcaaactggc ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac
    24181 cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcgt tcgtcaggag
    24241 cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc tttgccagct
    24301 tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg gcgataaaca
    24361 aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggtcttgcca
    24421 tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa
    24481 gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa ccggtatcgc
    24541 cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc gggagtacgg
    24601 tgtaatgggc ctgatatggg gattattcag cgtcattatt gcctcagttg cgcaattaag
    24661 cctgggtttt gcggcaagtc atctgccgcc gatgacgcac ttatgggatt ttatcgcggc
    24721 tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct
    24781 ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag cctatgcctt
    24841 gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg
    24901 gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat
    24961 tttcctgccc acgacaaaac aacgttactg agttttccct gccgctgtac actgccgttc
    25021 ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca gcttttcacg
    25081 attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa
    25141 gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataagtac
    25201 atgtctgtta tcttgtttat tgcagcacga ttttttgtgc cagccattcc attgcattat
    25261 cctgtttgct aagagttttc acatcaattt acagattaac agataaactc tttatcaacg
    25321 gcaatggaat ggcagaaatg agaagtgaaa cggagggtta attattgctg acgttgcacc
    25381 cactctttta gcgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggtaaa
    25441 gtcagccagc gcaccggttg ttgaaaacga gccagcttat ctttcaccca ttcgccaaga
    25501 tcaacggttt gctggtcata ctccaccacc gctaccggcc gatgaccaaa ctccttgtcg
    25561 gcaacggaaa cgataaacac ctgcaaaacc gcaggatgtg cagcaataac acgctcgact
    25621 tcttccggct gaataccctc accgccactg aagaacagat tgtctaaacg tccgacaatg
    25681 gtcagcttgc cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta
    25741 accagtgaaa ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc
    25801 cacacttcat tattaacgat tttcacttcc cgacccggca gcggcaaacc aacgtctgcc
    25861 aggccgtcgg cttctttcgc acataccgtg gaggcaaact cggtcagacc atagccgcaa
    25921 aagcaacgaa tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg
    25981 ccaagtaaca ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt
    26041 gttggcacca gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca
    26101 cgcaccgtca tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg
    26161 tgaaacagcg gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac
    26221 acaccttgcg cactggcaag atgggcctga taagtatgga cagcggcttt cggcaaaccg
    26281 gtagaacccg aggtcaacgt cattgagcac agacgcgtcg gctgccacgc agcggcatgt
    26341 acgccttcaa ccagctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc
    26401 ggcaccagcg caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc
    26461 agctgagggt tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaggcgagc
    26521 agcgtttgcg gcgtattcca cgcccgcaac atcacgccgc tgccctcaac cacaccctgc
    26581 gccgcaaagc ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca gttgagttgc
    26641 tcgtcattaa gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc
    26701 cagtcagaga agatcataac aactgctcca gtacatcagc gtccactaac ggcagtgggc
    26761 tccccggcca gcgacgaacc tgctgcgcct gcatcagatc cagcgtgtcc agtcccggaa
    26821 tggtgtctgg cgttaaccag gcagcaatcc gcgccagttg cgttagacct aagctcgact
    26881 caatggaaga actgatcacc gccgtcagcc ccagcgcatg cgccgcctgt acctgctcgc
    26941 gtactttttc cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt
    27001 cagccacaaa ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg gcaatgccgg
    27061 tttcacgggc aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga
    27121 tgcggtggcg ataatccggg ttaacatatt tggcaaactg ctgacctttc agcggtgtcc
    27181 aggcgcgatt tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattca
    27241 ccaccatgcc gtcgcgcacc gcttcgtaca atcccacttt gaccttcgcc actttctcgc
    27301 ccggcatatc ggcaagtttg aggatcagat cgtccggatc gccattacac agcggtgccg
    27361 cacggtagtt ggctgcttgc ggcaacgtat ctgtcagttc tgccaatgca cagcttacgc
    27421 caaaggccac ggaaggcatc tgcggtagct cgcaatcgcc tgccagccag ttatttaccc
    27481 aggcaagcag cacgctttgc gcctcttccc agttttcctg gctgaagccc ggcagtgggg
    27541 agatctcccc ccacccttcg cgctcgccct cacgcaggca aacgtacagc ccgtcgcggg
    27601 tttttaaccg cctgtcgcgc agaaccaccc ccgcgtccat ggggatctgc cagcggtata
    27661 cctgcgcgct acgcattacg gattccgttt aaatttgctg aaatcaggct gacgtttctg
    27721 gttgaaagcg ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt
    27781 gcccgccagc tcctgcagcc ccgcctgccc gtcacagtcg gcgttcagcg cagctttcag
    27841 gcagcgcagc gccatcgggc tgttttgcag catttcgcgg caccaacgga cggtttcttt
    27901 ttccagatcc gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc
    27961 gtcgtactga cggcacagga accagatttc tcgcgctttt ttctgcccga cgatacgagc
    28021 catgtaggaa gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa
    28081 gatggcatta tctgctgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc
    28141 gatggagtag ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg
    28201 gaagtccagc acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc
    28261 acgcactttc tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat
    28321 cacgccgata ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt
    28381 cagaggacgg aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc
    28441 ggtggatttt tcataacgaa tgtcctcgaa accttcggag cagtcgtgcc attcaaccgg
    28501 tgcgtaaggc attgcttcat caggataaat catattgtgt cctttagtca aaaacgcaag
    28561 atctgcgcca gacttgcgat tacgccagcg ggattttccc gatgcgcgtt atgtccggcg
    28621 cgaggaatga catggcagtc ggcagccagt tccgccgcca gggcgcggaa tttgctgtca
    28681 cgttcaccac ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca
    28741 ggctggacgg cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgttg
    28801 ctgcgcagcg ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt
    28861 tgataccagt cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg cgcccattgg
    28921 cgatcggaac gctgacgttc cgcacgttgt tcagcatttt gcagccccgg atgcccgcct
    28981 tcgataataa ccccacaaag ccccgccaac ccctggcaag ccgccatcat cgccacccgt
    29041 ccgccaagcg agtaccccac cagccagaag tcaaggatgt tgtaactaac caaggtttta
    29101 cgcagtaagt cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccatga
    29161 cctgggagat caacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat
    29221 tcgtggcaat cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggttttccg
    29281 tgttttgcct gcgcgtgcag gatcataaat ggcttacctg cgccagaagt tgctggagcg
    29341 tttgcgcacc atcggtgtcg ttaaccacca tttcaatcac cgtggtggtt ggcgtgcgcc
    29401 aggcgtcggc aaatgccgtt tcaagttcct gccagttttg cggacgatga tatttcagct
    29461 cgaacatcgc ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcac
    29521 gctcgctttg cggcgttggc aacagcgaga aaatttgccc gccgttgttg ttcaccacaa
    29581 ttaataccag cggcgcagaa acctgacgca ataacgccag cgcgttgaga tcgtaaagtg
    29641 cggagagatc gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgcctgcgg
    29701 cggtcgaaag cagtccgtcg ataccgctgg cccctcggtt gctgtacacc gggtaacctg
    29761 ccggaagttg cgaaagcgca tcaatcagac gtaccaccag gctgttacca acaaacaatt
    29821 gcccctgttc aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat
    29881 cacggcgggc aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc
    29941 agggctggcg tttttctgcc ggatgcagct ccagccagtc ggcaatattg ccaattaagc
    30001 gacgtccgcg atggtgtgcc ggatcaagtc gcccttcaat gtcatcaaca atccagtact
    30061 cttctggttc acagcttgcc tgccattgca ggagccgttt ccccgtcagg ctgcttccca
    30121 gttgcaccac aatttgtgcc tgctgcagct cgctggtcgc tttggcattg cctaaccaaa
    30181 gatcggcaca cggcagcggc tgcccggttt gtgacagcac atcgccaatc agcggccagc
    30241 caagagtttg cgcccacagg gcaacttttt tgccctcttc cgcactcatg cgcccggcaa
    30301 ccaccacgcc gcgcttttgt cgccagaaga accagtcgcg ctgtttttca ctttccagac
    30361 gaggcgcttc acgcagccac ggtttgtcgt cctgccacca gtcacccaga cgctgttgcc
    30421 agctaagccc ggtatcgtcc atttcgccat acagcggttc agcaaacggg cagttgatat
    30481 ggactccccc cgcatgaagc gtaccgagag cgtggtcgat ggtagaaacc agccaacgtg
    30541 cggggatatc ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga gaggcgaaca
    30601 ttcccggctg gcgaattgcc tgattcgcgc cgcagtcaat tagctccggc gggcgatcgg
    30661 cggttaagag aatcagtttt tctccggtta acccggcttc aatcagtgcc ggatagagat
    30721 ttgccaccgc cgtgccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca
    30781 gccccagcgc cagatgcccc aacccacgct catcgaaatg ggtgtggtga atgaatgcgg
    30841 aattctccgc cgccgctaac gttaacggtg tagaacgcga gcctggggcg atacagatgt
    30901 gtctgacgcc gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg
    30961 cgcttactga catgagtttg tccagtatca atattgcggc taagtataag gagctacaaa
    31021 aatagattat tgatatgaat cggtaatgat gcgactcatt actattccat ttgtaataaa
    31081 gtacgcagcc ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca
    31141 cggacaatgc ccgcgccagc atataatcgc acgacattgc cgctaatttt tgctgagcgc
    31201 agggaaacgc agaattcgct ttgttgtaat gagagatagc ccgcagaacc ggcgtaccat
    31261 tctcgggtga acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca
    31321 gcaactgctg ccgttggctg caactgatgt aaacagatca catcatccgc tttgttgagt
    31381 gaagtccaga tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt
    31441 aaaacatcca gcgtctgggt atcggcctgt aatcgttggc agatatcttc caccaccagc
    31501 atgttctcgc gctggttttt atcatccgcc atcagcctct ctcctaactg ctgcgcctgc
    31561 ttatcatcag gatgatttgc tactgttccc gccagcgctt cagtacgcag cgctttgtca
    31621 cgccgccgcc ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc
    31681 atgtaaaaat ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgcg
    31741 ttgaccggac ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt gtcgagctca
    31801 cctttggcga tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc
    31861 cagtgttgtt ctcgcgtggt ggttaaatgt aacccaggca agggcttgat actcaccagt
    31921 gtggcgataa attcttttgc ctgaatcgca tcgtgctgaa gggagctttc gctgaatagc
    31981 gtcaaccgca gcgtggcttt accgccacag cgtcgccatt ccaggcgggg taaaagtaaa
    32041 ttgccctgcg acgggtcaaa agcattcagc ccccaaatgc gtaagtcggc gtgttccggg
    32101 tgctggcgaa gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg
    32161 acggcagctt cttcatcacc attacgttgt tgccagtaga attgcgggta cgtttgctga
    32221 ctggccagcc agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc
    32281 cgaatgccgg gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc
    32341 gtagtaagtg attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact
    32401 acaaaaaaaa tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct
    32461 agtaccagcc cgacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag
    32521 tcatcagcac gataaacagc ctgcttcgcc cgatagtaat aactgtctga agcctggcta
    32581 acccgttttt tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc
    32641 tgatcggcgg gctcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc
    32701 aggtcgtcat cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt
    32761 aaaacctgtc actaactata gacaagagtt gttgattacg cctgaattac ctcgcgtgcc
    32821 atcccaatgt gtgggatacc atcttcttca tagacctccg tcacagggat aaaaccgaaa
    32881 ctctggtaga agttttgcag atgcgcctgc gccccgaggt aaacaggctt atcaggccag
    32941 tgatgcgtac atgtttcgag tgttttactc atcagctgct gccccacttt ttcaccacgc
    33001 aacgcttcac tgacaatcac ccgacctata acgaccggct caagatcatc atcacttttc
    33061 agaatcctcg catacgccac cagttcatca tttttccaac cgagaatatg gcgattatcc
    33121 cccgtcaaat catcaccatc gatatcctga taagggcaat tctgctcgac aacaaacacc
    33181 gcgcaacgca gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga
    33241 tcttgccatt caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat
    33301 tttttgaaac gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca
    33361 acccgcacgc gcaatgtcac ggcaatattg cttaacctgc aacatccgac ccagagcgga
    33421 ctttggttgt ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac
    33481 cctggaaaac tggacaagat ttttatcagt caccttcatg gcgatcatct ttttggttta
    33541 cccggcttgc tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt
    33601 ccgcaaggta tccgtgaatt tgtggaaacc gcgctgcgga ttagcggctc atggaccgat
    33661 tatccgctgg aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa
    33721 gtaaccgctt atccgctgga acacccactg gaatgttatg gctatcgtat tgaagaacat
    33781 gataaaccgg gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc gcctggccca
    33841 ctgtttcagg aattaaaagc gggcaaaaca atcacgctgg aagatggaag gcagattaac
    33901 ggcgcagatt acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc
    33961 ggcccctgcg atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg
    34021 acgctggata taaccatgga agccaaagcc aatagtcgcg gccatagctc tacacgccag
    34081 gctgcgacac tagcccgtga ggctggagtc ggcaagctaa tcattaccca cgtcagctcg
    34141 cgctatgatg acaaaggttg tcaacacctg ttacgtgaat gcaggtcaat tttcccggcg
    34201 actgaactgg cgaatgattt caccgtgttt aacgtttaac tctgcttatt attaaaaaca
    34261 gggtgaaact tgccctgtta tagcaacccg cgctacatat atccacccac acaggcatat
    34321 tgagcgattt ttgatattta ttcatctcct tacgcactat attcagagca ataagttgcc
    34381 tcttttcatc ttgattcagc acaacctttg caactaaaag gagcccatga tggttacagt
    34441 tgtcagcaat tattgtcaat tatctcaaac ccaactcagt cagacatttg cagaaaaatt
    34501 tactgtgacc gaggaattac tgcagtcttt aaaaaaaaca gcgttatccg gagatgaaga
    34561 aagtattgag ttactgcata atattgcgtt aggttatgat gaatttggaa aaaaagctga
    34621 agatattctt taccatattg ttagaaaccc aacaaatgag accctatcga ttatccgact
    34681 tattaaaaat gcctgtttaa tattatataa tttggcacat accgcaacca actcccacct
    34741 taaaccaact ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc
    34801 gaaattaatg acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc
    34861 aaaggtgctt ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa
    34921 aaatcgtcaa ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc
    34981 aaaaaatact caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca
    35041 cctggaagac gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca
    35101 gt
//
LOCUS       DADMFL010000032        34281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.032, whole genome shotgun sequence.
ACCESSION   DADMFL010000032 DADMFL010000000
VERSION     DADMFL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.032"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(93..2182)
                     /locus_tag="JNZ27_001542"
                     /pseudo
     CDS             complement(93..2182)
                     /locus_tag="JNZ27_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(2184..2435)
                     /locus_tag="JNZ27_001543"
     CDS             complement(2184..2435)
                     /locus_tag="JNZ27_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8663921.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2588..3151
                     /locus_tag="JNZ27_001544"
     CDS             2588..3151
                     /locus_tag="JNZ27_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY8663922.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3427..3849)
                     /locus_tag="JNZ27_001545"
                     /pseudo
     CDS             complement(3427..3849)
                     /locus_tag="JNZ27_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(3849..4442)
                     /locus_tag="JNZ27_001546"
     CDS             complement(3849..4442)
                     /locus_tag="JNZ27_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8663923.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVILCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4589..4996
                     /locus_tag="JNZ27_001547"
     CDS             4589..4996
                     /locus_tag="JNZ27_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY8663924.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5116..6108)
                     /gene="rpoS"
                     /locus_tag="JNZ27_001548"
     CDS             complement(5116..6108)
                     /gene="rpoS"
                     /locus_tag="JNZ27_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY8663925.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6171..7310)
                     /gene="nlpD"
                     /locus_tag="JNZ27_001549"
     CDS             complement(6171..7310)
                     /gene="nlpD"
                     /locus_tag="JNZ27_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY8663926.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNILKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7450..8076)
                     /gene="pcm"
                     /locus_tag="JNZ27_001550"
     CDS             complement(7450..8076)
                     /gene="pcm"
                     /locus_tag="JNZ27_001550"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY8663927.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8070..8831)
                     /gene="surE"
                     /locus_tag="JNZ27_001551"
     CDS             complement(8070..8831)
                     /gene="surE"
                     /locus_tag="JNZ27_001551"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY8663928.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8812..9861)
                     /gene="truD"
                     /locus_tag="JNZ27_001552"
     CDS             complement(8812..9861)
                     /gene="truD"
                     /locus_tag="JNZ27_001552"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY8663929.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9858..10337)
                     /gene="ispF"
                     /locus_tag="JNZ27_001553"
     CDS             complement(9858..10337)
                     /gene="ispF"
                     /locus_tag="JNZ27_001553"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY8663930.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10337..11047)
                     /gene="ispD"
                     /locus_tag="JNZ27_001554"
     CDS             complement(10337..11047)
                     /gene="ispD"
                     /locus_tag="JNZ27_001554"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY8663931.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11066..11377)
                     /gene="ftsB"
                     /locus_tag="JNZ27_001555"
     CDS             complement(11066..11377)
                     /gene="ftsB"
                     /locus_tag="JNZ27_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY8663932.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11571..11894)
                     /locus_tag="JNZ27_001556"
     CDS             complement(11571..11894)
                     /locus_tag="JNZ27_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY8663933.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11944..12549)
                     /gene="cysC"
                     /locus_tag="JNZ27_001557"
     CDS             complement(11944..12549)
                     /gene="cysC"
                     /locus_tag="JNZ27_001557"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY8663934.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12549..13976)
                     /gene="cysN"
                     /locus_tag="JNZ27_001558"
     CDS             complement(12549..13976)
                     /gene="cysN"
                     /locus_tag="JNZ27_001558"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY8663935.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13978..14886)
                     /gene="cysD"
                     /locus_tag="JNZ27_001559"
     CDS             complement(13978..14886)
                     /gene="cysD"
                     /locus_tag="JNZ27_001559"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY8663936.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15138..16175
                     /gene="iap"
                     /locus_tag="JNZ27_001560"
     CDS             15138..16175
                     /gene="iap"
                     /locus_tag="JNZ27_001560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY8663937.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVSKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16261..17412)
                     /locus_tag="JNZ27_001561"
     CDS             complement(16261..17412)
                     /locus_tag="JNZ27_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY8663938.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(17606..17758)
                     /locus_tag="JNZ27_001562"
     CDS             complement(17606..17758)
                     /locus_tag="JNZ27_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8663939.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18022..18756)
                     /gene="cysH"
                     /locus_tag="JNZ27_001563"
     CDS             complement(18022..18756)
                     /gene="cysH"
                     /locus_tag="JNZ27_001563"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY8663940.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18830..20542)
                     /gene="cysI"
                     /locus_tag="JNZ27_001564"
     CDS             complement(18830..20542)
                     /gene="cysI"
                     /locus_tag="JNZ27_001564"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY8663941.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20542..22341)
                     /gene="cysJ"
                     /locus_tag="JNZ27_001565"
     CDS             complement(20542..22341)
                     /gene="cysJ"
                     /locus_tag="JNZ27_001565"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY8663942.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22657..23022
                     /gene="queD"
                     /locus_tag="JNZ27_001566"
     CDS             22657..23022
                     /gene="queD"
                     /locus_tag="JNZ27_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY8663943.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            23100..24371
                     /locus_tag="JNZ27_001567"
     CDS             23100..24371
                     /locus_tag="JNZ27_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8663944.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLLALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24362..24622
                     /locus_tag="JNZ27_001568"
     CDS             24362..24622
                     /locus_tag="JNZ27_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8663945.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24639..25214
                     /locus_tag="JNZ27_001569"
     CDS             24639..25214
                     /locus_tag="JNZ27_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY8663946.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25362..26255)
                     /locus_tag="JNZ27_001570"
     CDS             complement(25362..26255)
                     /locus_tag="JNZ27_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8663947.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(26219..26998)
                     /locus_tag="JNZ27_001571"
     CDS             complement(26219..26998)
                     /locus_tag="JNZ27_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY8663948.1"
                     /translation="MNILLAFKAEPDAGMLAEKESQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26976..28391)
                     /locus_tag="JNZ27_001572"
     CDS             complement(26976..28391)
                     /locus_tag="JNZ27_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663949.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAE
                     FSPRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLR
                     WGTPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTA
                     FNSVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLA
                     HRKFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTD
                     IRSLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPET
                     KALPLVAAGNVGGANEHSVSV"
     gene            complement(28413..29867)
                     /locus_tag="JNZ27_001573"
                     /pseudo
     CDS             complement(28413..29867)
                     /locus_tag="JNZ27_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29937..30723)
                     /locus_tag="JNZ27_001574"
                     /pseudo
     CDS             complement(29937..30723)
                     /locus_tag="JNZ27_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31042..32319
                     /locus_tag="JNZ27_001575"
     CDS             31042..32319
                     /locus_tag="JNZ27_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8663950.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIDHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32346..33824
                     /locus_tag="JNZ27_001576"
     CDS             32346..33824
                     /locus_tag="JNZ27_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8663951.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVIGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8285 a         9069 c         8422 g         8505 t
ORIGIN      
        1 tcatcaatat cagtcatagt atttcctcgg ttatttcaaa tcagctttgt ttcctttatt
       61 taacagtccg ttcccgcgga ctgttttttt attcaggcca gcggatcatc ttcttcctgc
      121 atggcaaaaa gtttttgcat ccccaaccgg aaatcgcgtt caaattcttc gtcatgctct
      181 tcttcatatt ccggttgctg aactgccacc acattaccaa ccgggcgata aacattactc
      241 agccagggtt cagcctgttt gtgttccaga atctgaatac cttcatcttc agcatcacga
      301 attttttcct cagcgcgttt gcgcattcct ttaatacgct gctgttgttt gtgatattca
      361 gcactgacag caaaggctgc gcgtttattg ccgttccata tcgcctcaca gataaagctg
      421 ccgtccttgc gacgtaccgt aattcgctcg gcgtcatgaa tgtcataact gataagcact
      481 ttgcgaccgt gttcgtcacg caactccggc gcgtagtaaa tattgttcag ccactgaatt
      541 tcgcagcgtc ttaccggtcg ttccaccatt gggcggaaca tgtcgcgcag ctcaagatca
      601 ctcagccact caatttcggt gtcttcttct gccaggcgtt tttttctgaa ctctgtcggg
      661 ctgtaatgcc gtccgttcgg tttcatcggc agctcagaat gcgggcggtt gttgtaccat
      721 tccacgcctt cacgaatagc ctcaataagc tccgcccacg atggcagctc gcgcattgcc
      781 gctttctggc ggtcgttcag aggcttgtcc tgttgcaggg cgttaaacgc agatcacggt
      841 taagtacacg caacgattcc cggtcagcac ccttgccaaa ataggtgcga tacctcctgg
      901 ctatacgcat cggcagtgtc cggttcaggc gttcaataat gccgcgtccc tgtggattac
      961 cggcaatacc ggtagggtgt ttaatcccca ggcgtggcag aatacccacg acctctttgt
     1021 ccagcatgtc agccgtttca ccggaaccgt tatccgagta atacaggaac ggttttccgt
     1081 ggttgcgaat gccgtgctgt atcgcaccgg caacggcgaa cacgttttcg gcaaggtcaa
     1141 gactccagcc aacaacaaac cgtgttccac catcgatcac aaacgtgact tccggtgaga
     1201 atggacgccc gtggatcgga tgcgcacatt tcagcttcat accgtgaccg tcaccaatcc
     1261 agacataatt caccggcata gctgtccagt cgcggcgggt gaatccttca agctgacggt
     1321 attcactgcc ggtaacgcgc cctttttgtt tcaccacttc cggcagtttt ttcatggcat
     1381 accggacaac atcataggaa ggcatcacct ccagcatgta cggttcgtcc gcatggcgac
     1441 gctgccattc tgcgacaaaa tcctcgtagg cttctgacat gggtctgccg ttcacctggc
     1501 gatactgcgc cagaaattca ggcagccagc tgatttcttc cggttttatt tcgtcgcgtt
     1561 ttcccggtgc cagtaacagc agccgttcgg cggcgttctg cgactggttg tatttcagaa
     1621 cccactggta aagtggatcg cggctgatag tacgattagc gccacgtttt gcattggcgt
     1681 taactgcggc ctgttgcagg cgctccggca gctcaccttt acgggctttt tcaacaagaa
     1741 agttaatggc tttcagtcgc ccgaggttac cggcgctttc cagcttcagc acttccacca
     1801 ccagcgcagc tcttgcttca gatacccagc gctggttttc ggtcagcgca ttcaggcgtt
     1861 cttccatcag ttgtggagaa ccgcgatatg cttcgattgc ctggacagcg tcaggattgc
     1921 gtgtttttct ggtaacagct ttcacttcag aaacattgtt ctctgtagcc accagctgtt
     1981 ccacatagcg ttcacgcagt gcctttcgtg ttatttcagg cagacaatca atgtggtact
     2041 caatggcttt tgtgccttcc cgacggcgac taacgtcacc aagattacga gaaaaacgct
     2101 gtaacgcctg tcggatgcct tttgtggtgg ctggcattcc tggtacgccg agcaggtcat
     2161 ttacggtaac gtacgactgc atatcacgac gccttcctga taaatccatc gttacgatac
     2221 cggctgggcc agatgatttc tggcggtaca cctatagcgt cagaaattac gcgttcgtat
     2281 tttttgtttg gtgtgcgaac taccgctttc agtgaatcct gagcataacc agcatggagt
     2341 gaaagctccc ggaaggaaga ccacccgtta tctttgagcg ccagctgaat cttcagcgaa
     2401 ggccaatcgc ggtcactatc ttctctttgt ctcatctttc ctttaaccta caaaataacc
     2461 cgcgcggata tccgtatgga taaagattaa gataccaaag attccccata aagggttctt
     2521 tggtgctttt atggtttatt ttttaatttc attatttatc aatgaattag atagaaagga
     2581 accaagaatg agaaaagcac cccaaggttc ttttcctgcc gatggaaaag aaccgattat
     2641 cgaaagaatc tttaggttag tagaacgata cccttcaaga aatgcggctg ctcgggcatg
     2701 ggggatcaat gaaggcactt taaaaaatta ttacaacagg cgggattacg caccgatacc
     2761 acgcccgcat cagttaaaaa agatagcaga aagcgaaaat gttaccttgg aatggctcat
     2821 gacgggagag ggtgatgttg aactaaaaga ttccaaagaa cccaaaaaag agaatgcgga
     2881 taatgttgat gctcaaattc taatgttctt gtcatttctt aaaccacaag aaaaacaaca
     2941 agtagctgat gttcttggga gaaaaggagc ggagcaatta ctcattcttc tggatgagca
     3001 catccaagag ttgcacgctc tagttggagt tcgtagagcc atagcgttaa gccttggtaa
     3061 tttgccagac gaaaaggtta gagagattta cgcgttgtcc gaagctaagg gcgatcactt
     3121 aaatctacct cagaaagcag ccagcgcata acccagtgca agtgcctagc tgtgctaaaa
     3181 ttacattggt tttggcgctt gcacccatca gcgctataat aaacacctga atcgtttttg
     3241 aacggtcttt aaaggctatt gtaagaacct gtgagttatc ttgaatgttt tattgatatc
     3301 gaaccgttta acatttttgg actcgcttta tcttgaaaac cttgagttcc taacttgttg
     3361 attccaatct attcccgtct tttttaatct cttcccggtt atcttgaata aattacctag
     3421 tcatacgaga tagaaaccgc catcgccatc attcaggcga aacagcggca gaatatcgaa
     3481 caggttgatc tcttcgccat cgacggtgtt ctgcgcccag gctggattgg cgcggcgctc
     3541 cggggcaatc gggaagttat cccagcggcg gataaactca tcaatctgct ttttgaccgg
     3601 ggtgtttggt ggcaggccaa gggaaatcgc gtggttctgc caggaaccga tggtattcat
     3661 cgccacgcgg gcatcggtaa agccgcgaat attatcaaac cacagcgcgg gcgcgccgtc
     3721 gccgatacgc ccggtggcgt tagctgctgc tgccagatcc ggctcagcgt tcacttcttc
     3781 gctgattttc agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc gtaaatcatc
     3841 aaatgccatt attcattctc ctgagaaaaa ttccgggcct gcggcaatcc ttgccagcgc
     3901 ctggcgtggg gatgttcgag gccaaattga tccagcacgc gggctaccac atggtggaca
     3961 atgtcatcta ccgtttcggg atggttataa aaggcaggca tcggcggcac catcgccacg
     4021 cccatgcgtg aaagtgcgag catattttcg agatggatgg tgctaagcgg catttcacgc
     4081 ggcaccagca ccagtttgcg gccttctttg agcacgacgt ccgccgcgcg ccctaccagg
     4141 ccatcggcgt aaccagcgcg gataccggcg agcgttttca tactgcacag aataacgatc
     4201 atgccgtcag tacgaaaaga accggatgag atgatcgccg cctgatccgc cgggttatgg
     4261 ctgaagtctg cgagggcagc aacatcgcga gcgctgtaag gcgtttccag ttcaatgatg
     4321 gttttcgccc acttcgacat caccagatga gtctcgacat taggcatctc acgcagcgct
     4381 tgcagtaatg ccacaccaag aggcgcaccg gtagcccctg tcatcccgac gatcagtttc
     4441 attgcttatt ctcttaattg ttcgtatacg aacgttatta ttaacatact ccttcatttc
     4501 cacagcggca aggattgaaa agtaacaaca tcttcttcgt cactaaaaaa ccgctatgat
     4561 agcgacagag aattagcccg gagtttacat ggcgttacga aataaagcgt ttcatcagtt
     4621 acgacagctt tttcagcagc acacggctcg ctggcagcac gagttacctg acctgaccaa
     4681 accacagtac gcggtgatgc gcgccattgc tgataaacct ggtattgaac aggtggcact
     4741 gatagaagca gcagtcagca ccaaagcaac gctagcagaa atgttggcaa gaatggaaaa
     4801 tcgtggctta gtcagacgtg aacatgacgt cgctgacaag cggcgacgct ttgtctggct
     4861 gactgccgaa ggagaaaagc tacttgctgc ggcgatacct attggtgaca gcgtggatga
     4921 ggaatttttg gggcgtttga gtggagaaga gcaggagctg tttatgcagt tggtgcgcaa
     4981 gatgatgagc acatagaatg ctgattgccg ggtaggacgc tgacgtgtct tatccaggcg
     5041 acaaaaacac aagcttccca aacaaaagaa aaaggccagc ctcgcttgag actggccttt
     5101 ctgacagatg cttacttact cgcggaacag cgcttcgata ttcagcccct gcgtttgcag
     5161 gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg cgaacacgtt cacgggtgag
     5221 gccaatttca cgacctacat cttccagtgt tgccgcttcg taccccagca aaccgaatcg
     5281 acgtgccagt acttcacgct gtttggcgtt cagctcgaac agccatttga cgatgctctg
     5341 cttcatatcg tcatcttgcg tggtatcttc cggaccgttc tctttttcat cggccaggat
     5401 gtccagcaac gctttttcgg aatcaccacc cagcggggtg tctaccgagg taatgcgctc
     5461 gttaagacga agcatacggc tgacgtcatc aactggctta tccagttgct ctgcgatctc
     5521 ttccgcactt ggttcatggt ccagcttatg ggacaactca cgtgcggttc gcaggtaaac
     5581 gttcagctcc tttacgatgt gaatcggcaa acgaatagta cgggtttggt tcataatcgc
     5641 ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtt gagaagcgga aaccacgttc
     5701 cgggtcaaac ttctctaccg cgcggatcag ccccaagttg ccctcttcga taaggtccag
     5761 caacgccaga ccacgattgc cataacggcg ggcaattttt accaccagac gcaagttact
     5821 ctcgatcatc cggcggcgag aggcgacatc tccacgcagt gcgcgacgcg caaaataaac
     5881 ttcttcttcg gccgttaaca gtggtgaata accaatctca ccaaggtaaa gctgagtcgc
     5941 gtccaacaca cgctgtgtgg ctccctgcga taacagttcc tcttcggcca aatcgttatc
     6001 actgggttcc tcttctacta aggccttttc gtcaaaaacc tcaactccgt tctcatcaaa
     6061 ttccgcatct tcatttaaat catgaacttt cagcgtattc tgactcataa ggtggctcct
     6121 acccgtgatc ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc
     6181 ggcaaataac gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt
     6241 gttgaactgg ttccggtgct acccatggtt gctatttttt gccccgcctt cacttcttgt
     6301 tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac tcaggtaatc atcattatgt
     6361 ttgatgataa tcagattacc gtagccgcgc agcgcgttac cagcataaac aacgcggcca
     6421 tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga tatcaatccc cttgttgccc
     6481 ccctcagaag cgccaaaggt ttcgatcact ttgccctcag tcggccagcg ccaggtggag
     6541 ataggcgtac tggttgatgt actgctgaca gtcggctcgg ttgtgcttgc tgttggtacc
     6601 gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt tcggcaacat tttgttagca
     6661 ctctgttcac ccgaagactc agaatacgta attgtcggtt gcgacgcaac agcaacggtg
     6721 gaattttgtg caggcttgat cacaactcct tgctctgctg cgtcggcctg ggtaatagca
     6781 tttccgccag tgattggcgt accggaagca ttacccacct gcaaggtctg accaacgttc
     6841 agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt cacggaaatc gttgccagta
     6901 atccaggcga tatagaaaag tgtgtcacct tttttcacgg tataggtact gccgctataa
     6961 ctgcctttca gaatgttccc atactgacgg ttatagacga tgcgtccgtt ttccatctgt
     7021 accggctgct gagctactgg ctgcactggc tggatttgcg gttgttgagt agcctgaatt
     7081 tgtggctgct gtaccggctg aatttgcggt tgctgcgctg tagacgtcgt ccctattttc
     7141 ggcggcggcg taatcaacat accagaatta gtatttgcag gcgcattgcc attaacggag
     7201 ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg ccagccatag cgaaaccagt
     7261 gacaaagccg caatgcggcg aacggtgaat tttgggcttc ccgcgctcat ttatccccca
     7321 ggaaaaattg gttaataacc agtgacataa ttaccgtgca aggcacccta ctgaacactg
     7381 gaaaagatgt tcacgatacg ctgacctacg gcaaaataac caggaaaaat ccaggtattt
     7441 cctcacgttt taagccagct cacccttcac taaagggaca aagcgcacgg cctccacggt
     7501 atcgataata aattcgcctc cccgacgacg cacccgtttc aaatactggt gctcctcccc
     7561 tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc attagcgcag ttggaatttc
     7621 cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca cgtgcctgcc aaccttgcca
     7681 tccatcacca tgacgggttg aaacattatg taaatcaaga tttttcaggc ggcgacgtgc
     7741 ctgccactgc aagcctttaa tccgttcaac cgagcaaaca tgctggacaa gatgcgccag
     7801 gattgccgtt tgatatcccg aaccagtgcc aatttccagc acccgcgact gcggcgtcag
     7861 ctcgagtaat tcggtcattc gcgccaccat atacggctgc gaaattgtct gcccctgacc
     7921 tatcggcaag gcgatattgt cccaggcttt ttgttcaaac gcttcatcaa cgaatttttc
     7981 acgcggcacg gcggcaagtg cattcagcac ctgctcatcc tgaatacctt gcgcacgtaa
     8041 ttgatccaga agtgcttgta cgcgtctgct taccattgcg tgccaactcc cacgctgttt
     8101 aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta aatccacatg cagcggcgtg
     8161 atggagacat agccctcatc taccgcagca aaatcggtcc ccggaccggc atcacattta
     8221 ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg gatcttgctg cgggatcacc
     8281 tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa tacctttgat ttgatccaag
     8341 ggtaaatccg gaacgttaat attaagaata cgtccggtgc gcagcggctc tttacacagt
     8401 gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt aatgtttatg cccgtcaagc
     8461 gagacggcca gcgccggaaa acctaaatga cggccttcca tcgcggcggc taccgtaccg
     8521 gaataaataa catcatcccc cagattcggc ccggcgttaa ttccggacac aacaatgtcc
     8581 gggcgcggac gcatcagagc attcacgcca agatagacac aatcggtcgg ggttcccatt
     8641 tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca gggaggattc cagtgtcaga
     8701 gaatttgaag cgctgctgcg gttacgatcg ggggcgacca cctgaacgtc agcaaactca
     8761 cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta ccccgtcatc attactcagc
     8821 aatatgcgca taatcacctg ttgtgttgat aagttccctt acaacgctgg ttgcaaaact
     8881 acccgccgga agccagaaac ggatctctac ggtgacgtca tcccaccagt tccagcttaa
     8941 ttgttgcgga tacagcagca tcgctctgcg cgcggcttca actttttcgc gcaccagtaa
     9001 agcttgtaat tcagtttctg cggcgacagc tgcttgttcg aatgccagcg cttcacgctg
     9061 agttccccat tcgccactgc ccggcaatgc ggcggttatc atcagctctt tatcgttgac
     9121 gcgacgctgt aattccgcca gttcttcggt ggttgctaca aaccagctac cacgtccggc
     9181 taattgtagc gcatcgccgt caacaacttg attaacgtct gcttttttga ggcgctcagc
     9241 aacaatctga ttaaacaacg cactgcgggc tgccgacaac caaaaactcc gtttattgcg
     9301 atcgcgcacc ggagtattgg tttgcgccca gcgcagcgcg ccctgcaagt tgctaccgcc
     9361 aatcccaaaa cgttgggcac cgaagtagtt cggtacacct tttacgcaaa tatcgatcag
     9421 acgttgttca acgtcatcgc gattgctcac ttcgcgcaga accagggtaa aggcgttacc
     9481 tttcagcgcc cctaaacgca gcttgcgctt gtgccgcgca tactccagca cctggcagcc
     9541 ttccagttga aaggcgctca gatcgggcat ttccttgccc ggcacgcgag cacataacca
     9601 ctgttccgtt acagcatgtt tgtctttttg cccagcgaag ctgacttcac gggaatgaat
     9661 tttcaggaat ttcgccagtg catccgccac aaaacgggta ttgcagccgt ttttgaggat
     9721 tctaaccaga atatgctcac cttcaccatc aggctcaaag cccaaatctt ccaccaccac
     9781 aaagtcttcc ggattggctt tcagcagccc ggtgccttgc ggtttaccgt ggaggtaagt
     9841 gagattatca aactcaatca ttttgttgcc ttaatgagta gcgccaccgc ttcacaggca
     9901 atcccttccc cacgtccggt aaatccaagt ttttccgtag tagtggcttt cacgttaaca
     9961 tcatccatat ggcagccgag atcttcggca ataaacacgc gcatttgtgg aatgtgcggc
    10021 aacatcttcg gtgcctgagc gatgatagtg acatcgacgt tgccaagggt ataacccttc
    10081 gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc tgtcggcacc tttaaatgcc
    10141 ggatcggtat ccgggaacag tttgccgata tcccccagcg ccgccgcgcc aagcaatgca
    10201 tcggtcaacg catgaagcgc cacgtcgcca tcagaatgcg ccagcaatcc tttttcgtaa
    10261 ggaatgcgta cgccaccaat gataattggg ccttcaccgc caaaggcgtg tacgtcaaaa
    10321 ccgtgtccaa ttcgcattat gtattctcct gatggatggt tcgggtgagg taaaactcgg
    10381 ccagtgccaa atcttccggg cgcgtgactt taatgttatc cgcgcggcct tcgaccaact
    10441 gcggatgaaa tccgcaatat tccagcgccg aggcttcgtc ggtaatggta gcgccttcat
    10501 ttagagcgcg cgtcagacag tcatgtaaca gctcacgagg gaaaaattgc ggcgtcagcg
    10561 cgtgccataa gccgttgcga tcaacggtat gagcaatggc atttttgccc ggttcggcac
    10621 gtttcatagt atcgcgcact ggtgcggcga ggatccctcc cgtgcggctg gtttcgctca
    10681 acgccaacaa tcgcgcgagg tcatcctgat gcaaacaagg acgagcggcg tcatgcacca
    10741 atacccactg cacatcgcca gcggctttca gacctgccag cacggaatcg gcacgctcat
    10801 caccgccatc tacaacggtg atttgcggat gattcgccag aggaagttgt gcaaaacggc
    10861 tatcgccagg acttatggca atgacgacac gtgtcacccg gggatgcgcc agcagcgcat
    10921 gcaccgagtg ttcaagaatg gtttgattac cgattgagag atattgctta ggacattccg
    10981 tttgcattcg acggccaaat ccggccgccg gaaccacggc gcaaacatcc aaatgagtgg
    11041 ttgccatgtt aattcccggg ctgatttatc gattgttttg ccccgcagac tgtgcgcgct
    11101 tcgacgcgtc aggcaccaga cgataaaaag tttcgcccgg cctggtcatg ctgagttcat
    11161 tacgcgcacg ctcttcgagc gcctcctggc cgccattgag atcgtcaatt tcggcaaaaa
    11221 gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg cagtgccgcc acatcatcat
    11281 tgacgcgggt atagtcatgt ataccgttct taccgaacca cagcgaatac tgtagccaga
    11341 ccagaatagc cagcaacagc agcgttagtt tacccatcct gccccctgaa aaactgcatc
    11401 atcatcccat gcatccgaag acgactctac atcctctgtt ggggataccg cgacaacgcg
    11461 ggcaaatgta ccacatttgt ccattgttac gtatacccag ggcgtgcaga acataatctc
    11521 attattagtt acggtttgaa ttatgaacag aggagacaag aaagtacaaa ttagcccagt
    11581 agccacataa acagtgcgcc aaacataatg cctactgtca ccagggtgaa aacaatactg
    11641 tagcgtagct ttccgtccat caatgaatgc agcgcaatcc ccaccactac cgcgacaggc
    11701 atcagtgcca gaaagaaagg ccaggtgtag ataaagaaga acagcgtgtt agagccataa
    11761 atcaacatcg gcatcgccag cgcaaataac caggagataa aaccgaccac ggcaccaggc
    11821 agtgaccatg tagtttcttc atcctcagta aggctgtcgt tatttgttag tgtaatgtta
    11881 tggctattac gcatatttga tcctgttact ttgacgaacc gggcatggaa acccggtggt
    11941 gtctcaggat ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt
    12001 tgttactaat tgttcaccat tgagatgaat ttctgccgat tcaggctctt cgtaaacgga
    12061 atctattccc gtaaagttgc gcagttcacc ggcacgcgct ttcttatata agccttttgg
    12121 atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac acttcaataa agcgcccttc
    12181 tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag
    12241 caccaccagt ccggctgcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc
    12301 tttacgatcg gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cattgtcgcc
    12361 atccagcaga tacgtactga cgccgagttt atgtaacgcc tcctccagcg ccccggcgac
    12421 cgttgattta ccggacccgg agaggccggt aaaccacagc actacaccac gatgaccgtg
    12481 gtgtagttcg cgttgttgca cagtgaccgg atgactatgc cagacgacgt tttcgtcatg
    12541 cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc agtgcggaaa gtggcgacga
    12601 accagggcat tcaattccag ttcgaatgca ctgaattcag atgacgcagc tgttgcctgg
    12661 ctaactggct cgtgcaccat accggcaccc acggtcacat tgctcaggcg atcgataaaa
    12721 atcagcccac cggtaaccgg attttgctga tagcgatcta acaccagtgg ctcgtcaaaa
    12781 gtgagatcca ccaggccgat gccgttcagc ggcaggtttt caacttcgcg ttgggtaagg
    12841 ttattaatat caacctgata acgaatgcca tcaacacgag cacgcgtctt cttaccggca
    12901 attttgatgt cataactctg gcccggggaa agcggctgtt ccgccatcca taccacatcc
    12961 accgacgcac tctgcaccgc cggtaacgct tcgtctgccg ccagcagcag atcaccacgg
    13021 ctgatatcga tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa gacttcttcg
    13081 cgatcaccat caaaagtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcact
    13141 tttacacgtt gcccgacttc cacgcgaccg gatgccagca ttccggcgta gccacgaaaa
    13201 tcgagatttg ggcggttaac gtactgcacc gggaagcgca ttggctgggc atccaccact
    13261 cgctggatct ccacggtttc cagcacttcg agcagtgtca gaccgctgta ccacggcata
    13321 ctttcacttt gcgaagccac gttgtcacct tccagtgcgg agagcggcac aaagcggata
    13381 tccagattac ccggcagctg cccggcaaag gtcagataat cttcacgaat acgggtgaac
    13441 gtctcttcac tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc
    13501 cccaacagtg tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca
    13561 tcaatcagta agatcgccag ttcacatgtc gatgcgccag tcgccatatt gcgagtgtac
    13621 tgctcgtgcc ctggggtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg
    13681 taggccacat caatagtgat gccctgttcg cgctcagctt gtaggccgtc caccagcaga
    13741 gccagatcca gcttttcgcc ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag
    13801 agctgatctt cgtagatttg gcgggtatcg tgcagcagac gaccaatcag ggtacttttg
    13861 ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc
    13921 atccaggctt cgacgccgcc ttcattggcg atttgttgtg caagtacggt gttcatctta
    13981 aaaatacccc tgacgttttt tcagctccat ggatcccgcc tggtcgcggt caatcacgcg
    14041 accctgacgt tcactggtgg tggaaaccag catctcttcg atgatttccg gcagtgtttg
    14101 tgcatttgac tccaccgcac cggtcagcgg ccagcagccc agcgtacgga aacgcaccat
    14161 ccgttttttg atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa
    14221 cataccgtcg cgttccagaa ccggacgttc cgcagcgaga tacagcggca caatgtcgat
    14281 attttccagc cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaagac
    14341 gcggatgctt tcgcctttgt taatttgccc gttgtagttg tgccacagct ccgggcgctg
    14401 atttttcgga tcccagcgat ggaagcggtc acggaaagag tagattcgct ctttagcgcg
    14461 ggatttctct tcgtcacggc gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag
    14521 cgcctgtttc aggccttcag ttttcataat atcggtatgt ttcgcgctgc cgtgcacgaa
    14581 tggattaatc cccatcgcca cgccttccgg gtttttatgc accagcagct cgcagccgta
    14641 ggctttcgcc gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac
    14701 atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat
    14761 gacgctggaa tctttaccga tagagtagag catcaccgga tttgagaatt ctgccgccac
    14821 ctcgcgaata atgtggatgc tttccgcctc cagttgccgc aggtgagtaa gtcgtttttg
    14881 atccataacc gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag
    14941 ggagcgtaag agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac
    15001 gatttggcaa agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaag
    15061 atttgaaatt gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg
    15121 gtatttaagg actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg
    15181 gcgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata
    15241 cccaggcacg acatattgct actttctttc cgggacgcat gaccggaact ccagcagaaa
    15301 tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc agtgatattc
    15361 ggacatttaa tagtcggtat atttataccg cccgcgataa tcgcaagaac tggcataacg
    15421 tgacaggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcag atcatcatta
    15481 tggcgcatct ggatacttac gccccgctga gcgatgctga cgcagatgcc aatctcggcg
    15541 gactgacgtt acaaggaata gatgataacg ccgcaggttt aggtgtcatg ctggaactgg
    15601 cagaacgcct gaaaaatacg cctaccgagt atggtattcg atttgtggcg accagcggcg
    15661 aagaggaagg aaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga
    15721 aaaatacgct gctggtgatt aatctcgata acttaattgt tggcgataaa ttgtatttca
    15781 acagcggtgt aaaaacccct gaagcagtaa gtaaattaac gcgcgacagg gcgctggcaa
    15841 ttgcacgcag tcacggaata gccgcaacga ccaatccggg tttgaataaa aattatccga
    15901 aaggcactgg gtgttgtaat gacgcagaaa tattcgacaa agcgggcatt gctgtacttt
    15961 cggtggaagc gactaactgg aatcttggaa ataaggatgg ttatcagcaa cgcgcaaaaa
    16021 cacctgcctt cccggcggga aatagctggc atgacgtaag actggataat caacaacata
    16081 ttgataaggc acttccagga agaatagaac gtcgctgccg tgacgttatg cggataatgc
    16141 taccgttggt gaaggagctg gcgaaggcgt cttgatgggt ttgaaaatgg gagctgggag
    16201 ttctaccgca ggggcgggga attctaagtg atacccatca tcacatccag tgcgtccggt
    16261 ttacttccgg gaaaaccgca ccgtattgcg ccggtttatc tccgctggag cggggaacac
    16321 ctctggcgaa tagaaagcaa gccaggtttt gaccagtcga tcgtacatgg cattggccgt
    16381 tgccattgtt ggcaggccaa aaaacagccc ctgagcgtta cccgcagcca tcaacctgtg
    16441 cgccaggata agcgccgctt ctgttttacc tgcgccggtc acatcttcca gaataaacaa
    16501 ctgtggtcct ggctgattga tatccagatc cagcaccttt tgctgtaacg gtgtcgtatt
    16561 ctcaataaaa ggaaacagcg tattaattcc ggtgaaaggc gcggtttctg cttgtggcgg
    16621 aaagacggtt aatgcgtttt gagcctgaac taaagccttt tgccagtaat cttcaatatc
    16681 cattgcctgt gcgacgcgtg gaaaaaatcg cgttgacgac cctgtccagt ctgcaagtac
    16741 gacggttgca gagatatacc aggaaagttg ttttataagt tcaacgccct cgtcatcatc
    16801 ccagaatgtg gggatctcta ttcgcggaaa cagtggcttg atttcaagga gaaaatctcg
    16861 tgctgcagct ttgtcttcag gcagaaaatt atccaattca tccatacgat cagggggccg
    16921 accatgatgc cctgtcgtca cgggcatcca catttctatc actcgcctaa gtttgcgcgg
    16981 agagagcgaa gatgaaggaa gtaactcctg acattcactt aaataatgat tccacaacca
    17041 gtagcccagc gttgaatgag agattttttc gtaatttttt ctggcacctt cctgaatctt
    17101 gaattcaggg ggcaggtaaa gttgttgaaa agagcgggca aattttccaa tatcgtgcca
    17161 gcacagcaac caggcgaaaa attgagctgc ctgttccttg tcagaaatcc ctaattgacg
    17221 aaaataatca gccagcccga agcaatttct tttaaccatt aaatagccca ttgcggccac
    17281 atccagcaaa tgccagcaaa gaaggtgata gtcgtcgcta ccctctttct cgccacgtcg
    17341 gttttttccc cagaaatcaa agaaagtcac aatattttta tccttcagta aacttaaagg
    17401 atatttacgc atattttaaa aactatctgt gatatatatc agccaataaa acatttacat
    17461 tatgaataaa ttaatgattt tcatttgaaa ttcataataa taaatagaga ctatatattc
    17521 acataaaaaa tataaatttt aaaaaagggt gcaggagacc aaaccatcac aattcagtac
    17581 gcggggtttt acccccgcgt ggtggctact tcttcgattc gtaagcgaga actgccttaa
    17641 actccatacc acgttctctg atactcaact cacataacga gtcacctacc atcaacgtga
    17701 atcccagtac tgtacaacac agtacgatga ttgccacaag ggcatatttt gtcagcatgt
    17761 cttgcctccc tgcaaagaag agactaacat ccgaattgct acgttcgaac gcgagcctca
    17821 ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa tgcttaacgc
    17881 cagacagcct caagcaccca acgccattct atacctgtta attttccctg cgatgttcac
    17941 ctttaaatgc cggaatgctg ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc
    18001 gtttaattta tccggcaata tttacccttc gtgtaatcca cattcccttt ttaagccaaa
    18061 gaaacgcgtt tcttcttccg ccatgccggg ttcccattta cgggttgtat gggtatcgcc
    18121 caccgataaa tatccttcgt cccataatgg gtgatatttc aggccatgtt tttgcaggta
    18181 ctgataaata gttcggttat cccagtcgat aatcggcagc actttaaata cgccacgctg
    18241 aattgccagc accggtaaat tggcacggct gccggattgt tcacgccgca ggccagcaaa
    18301 ccacgtttgc gcattcagtt ctttcagcgc ccggttcatc ggttcgactt tgttgatgtc
    18361 attgtacttt tcaatgcctt caacgccctg ttcccacagt ttgccgtagc gtgcttcctg
    18421 ccaggcggcg ctttcggtag cgcggtacac tttcaggttg agcttgagtt tgtccgttaa
    18481 ctcgtcaata aagcggtagg tttccgggaa cagatagccg gtgtcggtga ggatcaccgg
    18541 aatatccggg tgaatctgat tcaccagatg caggctcacc gccgcctgaa tgccaaagct
    18601 ggaagaaagt acatattcac cgggcagatt atccagcgcc caggcaacgc ggccttcagc
    18661 gtccagtttt tccagttcgg cgttagtttc tgccagcgcc agaatgcgat ctaccttcgg
    18721 cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt ttgccttgcc
    18781 tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt taatcccaca
    18841 aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga
    18901 agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca
    18961 ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt
    19021 tgccgccaag atgcaggttg tagcgacccg gtgctttacc caccaggccc acttccgcca
    19081 gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc
    19141 tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct
    19201 ccgcttccgc catcgccagc gggcaagtcg ggaatgacac gcaggccatc gagttttcac
    19261 gctgcggcgt gacggcattc attaacccgc tctctttggc aattttctcg atcttcgctt
    19321 tctcgctttc cggcacaccg gcgatgatca gattctggtt cgccgtaatg cggaaatcac
    19381 ctttgtggat cttcgcgatc tccagcaggc cagttttcag cggacgcccc ggataatcaa
    19441 ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc
    19501 agccaatacg atcgccgcgt cctgtaaatt catacgggcg gatcggttca aatttgatcc
    19561 ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc agtgtgtatt
    19621 ttgttttggc atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg
    19681 cttccgccac cgctagcgta tgctctagcg gcagatagcc aaactcactc gccgtgcggg
    19741 cgtaggtttt cttgttaccg tgttcgatag aaagcccacc gcccaccagc aggttgaagc
    19801 ccaccagctt gccgttttcc gcgatcgcca cgaagttcat gtcgttggcg tgcagatcaa
    19861 tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg taggtctgac
    19921 caaggatcgg ttcttcatcg gtggtggcga ctttttcctg atcgagccag atctccgcat
    19981 acgcgcgggt acgcggcagc agatgttcag aaatcttctt cgcccactcg tacgcttctg
    20041 cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttag
    20101 cagtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct
    20161 ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg ctgccataga
    20221 tggtgttttc accggcaaat ttatcgatcg cctgccactg tttggtggtg ataactccac
    20281 ccggcagacg gcagcgcaac agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt
    20341 cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt
    20401 tatcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca
    20461 ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag
    20521 gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag
    20581 ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc
    20641 cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg
    20701 ggcaccgtca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt tatcttgtac
    20761 gtaaactttt tctttttggt cgcgcgacca ggcgagatcg atccgcgtca gcacaccctc
    20821 tttgacgtag cgctgccact ccacctgata caggaagtct tccgtaaaat gcggattgcc
    20881 aaagaacagc cagtttttac ctggcgcttc gtcagcggcg cgttgctgca taaaggcgcg
    20941 gaacggcgca ataccggtgc ctgggccaat cataatcacc ggggtttcca ggttagcggg
    21001 caggcggaag ttatcgttat gttcgataaa tacgcggact tcgccctctt cttccacgcg
    21061 gtcagcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt cgtaacgcac
    21121 cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg caatggaata
    21181 gagacgcggg gtcagcgggc gcagcagatt aattagcgct tcggcatcaa gctgtgccgg
    21241 ggagaaacgc accatgtcaa caatcagcgt cgtcgcggcg taatgctgta acttcgcttt
    21301 atcgcccacc agcggcagca gtgtttcact gcgggtaagc gtggcgtaat tctcaacaat
    21361 gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt
    21421 tttgccctcg acagtgacag gttcatcgcc tgtcagccac agcagttcga caagttcttt
    21481 caccagtgcc ggatcgttct gataccagac gcccagcgcg tcacccggct ggtaacgcag
    21541 gcccgagtcg cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt tacgcccagt
    21601 aattttctgg ttaacagaga ggctagccac cagcggcgcg tctttgctgt acgggctggt
    21661 gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg
    21721 cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac
    21781 atcagaatcg acacggtcga gcaggcgttc accacccagt tccgccagct tgctgtcaaa
    21841 atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa acacggcaaa
    21901 cgcggtgttt tccagctttg gcgctttttt ggagaacagg aacttatgca gcgcgacggc
    21961 ttcttccggc ggttcccctt ccccttgcgt tgacgtcact acgatgagca gtttttcgct
    22021 ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgctgc
    22081 taataaatca tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt gcgaagcaga
    22141 gataatcgtt atacccggca tttctgcggc gggcgctggc gtcactgcaa gcgcaacagg
    22201 ctgctggttg agcacgcccc agaaatagcc agaaacccag gcaagctggg taggcgttaa
    22261 atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga
    22321 aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc
    22381 ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg gtgtggatta
    22441 aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca atagtcttta
    22501 acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa tcattaagat
    22561 aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg
    22621 tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac gttatttaaa
    22681 gatttcacct tcgaagccgc tcaccgctta ccacatgtcc cggaagggca taaatgtggt
    22741 cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat
    22801 acgggctgga ttatcgattt cgctgaactg aaagcggcgt ttaagccaac ttatgagcgc
    22861 ctcgatcacc attatctcaa tgatattcca ggtctggaaa acccaaccag cgaagtttta
    22921 gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta
    22981 aaagaaacct gcaccgcagg gtgtatctat cgcggcgaat gataagagtg tgtcggcggt
    23041 caatttccct ttagtaacgc tatgttaggg tgttgtgttc tggatatctg gggcatgaca
    23101 tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc gcttgcgcgt
    23161 tacgctgcgc gcgagcggat ttatccgttt tgttactcga acgcgctgaa atccccggca
    23221 gcaaaaatct ttccggcagg cggttatata cccatgcact cgcggaactc ctcccgcaat
    23281 ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc ctgttaacgc
    23341 cggatggcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac
    23401 gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc
    23461 ccggagcgac ggtggatgca ctgtatgaag aaaacggcag agtctgtggc gttatttgtg
    23521 gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg
    23581 ctgaacgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg gggatcaaag
    23641 aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag aataacgaag
    23701 gcgcagcgtt gctgttcagc ggcggaatct gtgatgactt acccggcggc gcatttcttt
    23761 atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa
    23821 gtcgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca gtgcgcacgc
    23881 ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc
    23941 acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca
    24001 gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact ggcgcacagg
    24061 cggcggcaca aacgctaata agcgcctgcc agcaccgcga gccgcaaaat ctgcttgcgc
    24121 tttatcatca caacgtagag cgcagcctgc tatgggatgt tctacaacgt tatcagcatg
    24181 ttccggcgct tttgcaacgc cctggctggt atcgggcgtg gcctgcgtta atgcaggata
    24241 tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc cagttattct
    24301 ggcgtcattt acgtcgtcat ggcctgtggc atctggcggg cgatgttatc aggagtctgc
    24361 gatgtctgta gcccgtaatc tctggcgcgt tgctgatgcg ccgcacattg ttccggctga
    24421 ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggtag gtcttttttc
    24481 tctcacacag gaaggtggct tacgtattga ctatcgcggc tgcctggagt gtggcacctg
    24541 ccgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt ccggattcgg
    24601 catcacctac cgctttggat aaataataag gataatttat gcccctctta cacctgctcc
    24661 gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg gccatcgatt
    24721 ctgaatgcca gtttatttcc gtgttgtacg gcaatatctg caccatcagt aatatcgtta
    24781 aaaagattaa gaacgccggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt
    24841 caaataaaga agtggtgatt cagtttttga aactggtgac cgaggcggat ggaattatca
    24901 gcaccaaagc atcgatgcta aaagcggcaa gagcggaagt ttttttctgt attcatcgcc
    24961 tgtttattgt tgattcgatt tcgtttcaca acattgataa gcaagttgcg caatcgaatc
    25021 cggattgtat tgagatcctg ccaggctgca tgcccaaagt gctgggctgg gtgacggaaa
    25081 aaatccgcca accgctgatt gctggcgggc tggtatgcga tgaagaagat gcgcgtaatg
    25141 cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggagtctgg acgttagcga
    25201 aaaaattgct ttgacgggat aatcataacc aattgaattt gttttgattt gttggccgca
    25261 cgccacatcc gacattcagc gcctgatgcg acgcttaacg cgtcttatca ggcctacagg
    25321 tccccagtcc agaaccgtag gcaggatcat ccggcagcac gttattggta ttctgcgtga
    25381 atgtttgtca ccagtgcctc cagcaccact ttccagtcat caaccacgtc aatatctgcc
    25441 tgtgaaaata ccgctgcgct ggcgtcatga ttaatcgcca ccataaactt gctattgcgt
    25501 acgcccgcca ttaacgctgc tgcgccggaa gcgccaacaa caatgcacac ctcaggtgcc
    25561 agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt catcactcgc
    25621 gcccgactgt agcccacttc ggttcccagc ttttctgcca gcataacgat ttcctggtta
    25681 tctgcctctc cgccctgccc caccaccagc acccgtctgg cctcagctaa tgggtcgcgg
    25741 ctaacatttt tcagatcctc tgtgctaacc agccagtcgg gaagcgcacc tgggacaatg
    25801 ttcagttgct gcattccaga gggcaacgtg gcgtttttcg ctgctcccgc ctgacgcgcc
    25861 agggaaaggc ataacgggcg tttttctgtt tgcagcgtcg ccgttaacgc atttccccag
    25921 tgggatttgc gcatgcttac ggttggaata tctaacgaag tcacctgaca gatgcttgca
    25981 ccatgtaaac gccaggccag ccgggtcgac agttcatcgc caaacgttcc cggcggaaag
    26041 agaacaacgt ccgcaggcgt tcgctgccac tgttccacca tcgcatccag cacctgttcc
    26101 gccactacgg gctgaggttc tatttgccag tgcgccagcg tacaaccgct gaaatcctgc
    26161 gccgccagcc agctcgctat tgcggcattc tcctgattta ttgtcacaat tgcgatattc
    26221 atggctgcat cctttggcgt aaatagtcct gccatagctt ttgagctttt tctgctaccg
    26281 tttgcccatc aatcagcgtc gctccgcatc gttgctctgc tcttgccagt tgcagacact
    26341 gcatcgctgg catttcggca gcaacagttt tacggataat ttctgctttc cccgcagcca
    26401 ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc acggcaatca
    26461 ccgcaggcaa gcgaacccgg caacagcgca acccatgttc agtacgctgt tcgagggtga
    26521 taaacagcgc gtcgagagtg aaacgctcca cctgggtgaa gcagggccag cccagcattt
    26581 ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag ccggtaatta
    26641 tcagatccag cggattctga cgctgccatt cggcaatatg gcgagcgaca aattccggcg
    26701 caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca agagccatga
    26761 gatagcgcag ccagtgcaac gcccgttcat cccccatgct caacgcggtt aaagacatcg
    26821 gcgtaccgtt ttttctctgc gctaacagca gcgcggcagc ggcctgttca tcagcaccga
    26881 gtaaacttcg cagtagcgaa acatccggtc cgctattacc ctgagccgcc gcctgcgact
    26941 ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga atgttcattc
    27001 gcacctccta catttcccgc cgccaccagc gggagggctt tagtttctgg tgcccatagc
    27061 caggtcacaa caaaaccaac caacaacact gtcgccagga gcaataaggt gacttgcatt
    27121 ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcgcc aagtcgactc
    27181 atggcggtgg caaaaccaac gcccagcgag cgaatgtcag taggaaaact ttccgcaggc
    27241 aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac aaaaagcagc
    27301 agcgttaatg aactgccgga aggcaaacag gccattacta ccagcgttgc cgccagcagc
    27361 aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac taatcccagc
    27421 agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc atcttccaga
    27481 ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg gattacgagg
    27541 cagacaaaga agacgctgtt aaacgccgtg cgccgccagt aacgcgaaga gaacaaggtt
    27601 ttgatatgtt tatgggtcgc cgttaccact tcatcgccca gtaaaacatg gggaccaaaa
    27661 tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcacagtag ccaacgtggc
    27721 gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc tgccgatgcc
    27781 agcaaccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc aatacttgcc
    27841 aacacatagc ctacggtcca caccacgctg aatgcgccca gcaaaatacc gcgatggcgg
    27901 cgcggggaaa attcagccag caaggtgtga cctactgagt aatcgcctcc cagaccaatg
    27961 ccgatcaaaa tgcgcagccc aataagatgc tctggcgtgg tggcaaaaaa ttgcaagaac
    28021 gaagcgagcg taatcagcaa aaagctgaag gtgaaggtga agattttttg ccgaccaata
    28081 tggtcggaga tccacccaag aaccaggctg ccaagaaaca aaccaaggag cgccgagccg
    28141 ccgatcattc ccgccataaa cggcgtcagt tgcatggcgg gcgtaagttg aataatggcg
    28201 taaccaatga cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca
    28261 attcgacagt gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc
    28321 tttacccctt cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa
    28381 cgccccgtca tcgcggaacg ctgcctgata cattattttt caatcggata gatagtaccc
    28441 gtattcataa tgccgttagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg
    28501 ctgccgtgtt ccagcttgct ccagtgaacg cgatgtttac caatgccatg gtggtgcacc
    28561 atcgaaccgc cgaggcgaat ggtttcttcg cagatgattt tgttgagcgg attgtagtac
    28621 ttgtcgattt cctcttccgg cttacagtca acaacgttgt agtcatagac gaagtacatg
    28681 ttggtgccgt tctgatagct atgagaggag tgaccgccca gcatggtgat gtcgtcggcg
    28741 tgcgggaact cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc
    28801 cagcagccgg acacttcggt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt
    28861 tcggcagcca ctttatccgg tccccagttc aggttgttaa accaggtttc aatcagtttg
    28921 ctgtccacgc gctggcactg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc
    28981 gccttcgcaa tgcgagggtt accttcagcc ataaagatca gcacccattt tccgtcggca
    29041 aaatgggtga agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga
    29101 tacccttcca ccatgatttc acgcaggatg ttgaagccgg ttttcatgtc ttccaggata
    29161 tagccgtaga agaggttgtt ttccggggtg tatttaaaga ttttcactgt tacttcagtg
    29221 atatagcaca atgcaccttc gttgccgatg atgatgtgac gaatgtccgg gccagccgcg
    29281 cggcgtggca cgtttttaat gcgtgtgacg gtgccatctg ccaacactgc ctccagacca
    29341 acgaccatat cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct
    29401 accaggccgc ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct
    29461 ttttcacgca acgcgttttc cagcacttcc agcggaacac cacattgcgc cgtcgcctgc
    29521 atattctcaa tatcaatatt aatgatttga ttcatggcgg agccgtcgag caccaccgag
    29581 ttttctacaa cagtttccag cccaccttcg gtggcggaag caccggtacg cggcacaccg
    29641 ttaattttgt gcgcattcat aaaattcagc acacgggata cttactctgt ggaaccgagt
    29701 tttacgaccg ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga
    29761 aaacggtcaa tactgttttt ctttaatact gtttcatcgg taattacgcg atctgcacca
    29821 acaatttcct ttagctggtc gacaatcgct gcgcgagata aagacatagt aattccttcc
    29881 tgataataaa aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag
    29941 cgcactaaat aaccgccatc gaccactaat aaatgcccgt tgacataatt cgatgcctga
    30001 cttgcgagaa acacggctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga
    30061 atatgatcaa gaacgcgctg attggtttct ggattactgc gtgtcgccag cataatatgc
    30121 tgttgcataa tagccagggg cgataccatt tacctgaata ttatattgac ctagttcatc
    30181 acaataagct ttggtgaacc cggcaagagc atgtttagtg gcagaatatg caggtgacca
    30241 ttgtccacct aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg
    30301 gatcataatt tttgccgctt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat
    30361 catcggatcc cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc
    30421 attgttaacc agaatatcga cagtaccgaa gcgctcacag caggcagcga taatcttctg
    30481 cggtgcgcct tctgcggtaa tatccacctg catgaagtcc acttcaacac cctgtttttc
    30541 aatcatttcc tttgtttcgc cgttatcttt gacgaagcta ggaataaaaa tatttgcgcc
    30601 agctttggcc aacgccattg caaatgcctg acctaaaccg ctattcccgc cggtaacaat
    30661 tgctgtttta cctttcaggg agaaaaaatc cattgagaac gcattgagag attcgattga
    30721 catagttagc tccaatttcc gtaaggcaaa aaaaaagaaa ggtaacttcc cccgcgagag
    30781 ggaagttacc tttctcatta tctctggtaa ctggagataa tttttagcat aacggcataa
    30841 ctgctaatgt gatcgtaatc acagtgtgat attcgtattg tcaaatagtt ttaaaaagca
    30901 gaagcgtgat tactctcaca caacacctct gctttccatg ttatttctta ttgcaattac
    30961 tagagacctg agaaaagtaa tccacctgaa agggcggctt acttttctct tttttttgtc
    31021 tattacaaat gggaatacga tatgcaacac aactcatatc gccgttggat aaccctcgcg
    31081 ataattagtt ttagcggcgg cgttagtttt gacctggctt atttacgtta tatttatcaa
    31141 attcccatgg cgaaatttat gggattcagc aataccgaga taggtttaat aatgagtacc
    31201 tttggtattg cggctattat tctttatgct cccagcggcg ttattgccga taaattttca
    31261 caccgcaaaa tgattacttc cgcgatgatc attaccggat tactgggtct gttaatggca
    31321 acgtatccac cgctgtgggt aatgctctgt attcaggtcg cctttgcgat aacgacgatt
    31381 ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc ttggcgatca tagcgagcaa
    31441 gggaaaatta tgggctggat ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg
    31501 tttaccatgt gggtcttttc tcgctttgca ccggatgaca gcgccagcct gaaaacagtc
    31561 attatcatct acagtgtggt ttacatcttg ttggggattc tgtgctggtt ttttgttagc
    31621 gataacaaca acctgcgcag tgccaataac gaagaaaaac agtcattcca gcttagcgac
    31681 atccttgccg ttttgcgtat cagcaccacc tggtattgca gcatggtgat ttttggtgtc
    31741 ttcacaatct acgccattct gagttactcc accaactatc tgaccgaaat gtacggcatg
    31801 tcgctggtgg cagcgagcta catggggatt gtgatcaaca aaatcttccg cgcgctgtgc
    31861 ggcccacttg gaggcattat caccacctac agtaaagtga aatcccctac ccgcgtgatt
    31921 caaatccttt ccatcatcgg tttgctggcg ttgacagccc tgctcgtcac gaactctaac
    31981 ccgcaatcgg tcgcgatggg gattggcctg attttactgc tgggattcac ctgttacgcc
    32041 tcacgcgggc tgtactgggc ctgccccggc gaagcgagaa caccgtctta cattatgggc
    32101 accacggtag gtatttgttc ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt
    32161 atcgaccact ggcaagacac cctgcccgct gcagaagcct accgcaatat gtggctgatg
    32221 ggcatggcgg cgcttgccat ggtgatagtc tttacctttt tgctgttcca aaaaattcgt
    32281 actgctgata gcgcccccgc aatggctagc agcaagtaag ccaggaaaat tacaggagaa
    32341 aaatcatgtc gaaaaaatac atcataggga ttgatggcgg aagtcagagc acaaaagtgg
    32401 tgatatacga tctggaaggt aatgtggttt gcgaagggaa aggcttattg cagccgatgc
    32461 acacgccaga tgccgatact gcagaacatc ctgacgacga tttatgggca tcattatgtt
    32521 ttgccggtca cgatttgatg agtcagtttg ccgggaataa agaagatatt gtcggtattg
    32581 gtctgggatc catccgttgc tgccgtgcgt tattgaaagc cgatggcacg cccgctgcgc
    32641 cgttgattag ctggcaggat gcacgcgtaa cgcgtcccta cgaacacact aatcctgacg
    32701 tggcatatgt cacctctttt tcgggttatc tgacgcatcg cttaaccggc gagtttaaag
    32761 acaatatcgc caactatttt ggtcagtggc cggtggatta taagagctgg gcatggagcg
    32821 aagatgctgc ggtaatggag aagtttaata tcccccgtca tatactgttt gatgtgcaaa
    32881 tgccaggcac tgttattggg catatcacac cacaagccgc gctggcgaca catttcccag
    32941 ctgggctgcc ggttgtttgt accaccagtg ataaaccggt agaagctctg ggggccggat
    33001 tactggatga tgaaacggcg gtgatttcac tcggtactta tatcgcgctg atgatgaacg
    33061 gcaaagcctt gccgaaagat ccggtggcgt actggccgat tatgtcttct attccgcaaa
    33121 cattgctgta tgaaggttac ggtattcgca aaggtatgtg gacggtgagc tggctgcgcg
    33181 acatgttagg cgagtcgtta attcaggatg ccagggcgca ggatctttca ccggaagatt
    33241 tactcaacaa gaaagcctct agtgtgccac ctggctgtaa tgggctgatg acggtgctgg
    33301 attggctgac caatccgtgg gaaccgtaca aacgcgggat tatgatcggc tttgattcca
    33361 gcatggatta cgcatggata tatcgttcga tactggaaag cgtggcgctg acgctgaaga
    33421 acaattacga caatatgtgt aatgaaatga atcactttgc gaagcatgtg atcattactg
    33481 gcggcggttc gaacagcgat ctgtttatgc agatttttgc cgacgtgttc aaccttccgg
    33541 cacgacgtaa cgccattaac ggttgtgcaa gcctgggggc agcgattaat acagcggtag
    33601 gtctggggct atacccggat tacgcaacgg ctgtcgacaa aatggttcgc gtgaaagata
    33661 tctttatgcc tgttgagagc aatgccaaac gctacgacgc gatgaataaa ggtattttca
    33721 aagacctaac caaacatact gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg
    33781 aattggggaa tgcggattcg atccagagct ggtcgaatgc gtaagtgagg aagaccgggc
    33841 gggaaactgc ccggcctgaa tatacctgaa tggttatccc cgctgacgcg gggaactcga
    33901 ataactgacc aacgtctggc tctctaaatc cggtttatcc ccgctggcgc ggggaacact
    33961 ctaaacataa cctattatta attaatgatt ttttaagcca gtcacaatct accaacttta
    34021 caggatcaca caaacaacac atccattatg ttaaagagca cttaatccat tgattaaaaa
    34081 ggtaaatatt taaaataact ctatacaaac taaaatctac caaacttcgc cgcaataatt
    34141 ttcactccag caaaaaatta acgccgcaga atttgtagag aatgataatg attagtccgg
    34201 gatgggcaag atggatgagt tttcaggggc gacaaataat acaaatgagt ggaatttttt
    34261 acaagttcca cccatttata a
//
LOCUS       DADMFL010000033        33788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.033, whole genome shotgun sequence.
ACCESSION   DADMFL010000033 DADMFL010000000
VERSION     DADMFL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.033"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..2003
                     /gene="speF"
                     /locus_tag="JNZ27_001577"
                     /pseudo
     CDS             1..2003
                     /gene="speF"
                     /locus_tag="JNZ27_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2000..3319
                     /gene="potE"
                     /locus_tag="JNZ27_001578"
     CDS             2000..3319
                     /gene="potE"
                     /locus_tag="JNZ27_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY8663952.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3375..5015)
                     /gene="pgm"
                     /locus_tag="JNZ27_001579"
     CDS             complement(3375..5015)
                     /gene="pgm"
                     /locus_tag="JNZ27_001579"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY8663953.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5041..5586)
                     /gene="seqA"
                     /locus_tag="JNZ27_001580"
     CDS             complement(5041..5586)
                     /gene="seqA"
                     /locus_tag="JNZ27_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY8663954.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5771..6535
                     /gene="ybfF"
                     /locus_tag="JNZ27_001581"
     CDS             5771..6535
                     /gene="ybfF"
                     /locus_tag="JNZ27_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8663955.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6606..6968
                     /gene="ybfE"
                     /locus_tag="JNZ27_001582"
     CDS             6606..6968
                     /gene="ybfE"
                     /locus_tag="JNZ27_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY8663956.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7108..7638
                     /gene="fldA"
                     /locus_tag="JNZ27_001583"
     CDS             7108..7638
                     /gene="fldA"
                     /locus_tag="JNZ27_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY8663957.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7848..7934
                     /gene="uof"
                     /locus_tag="JNZ27_001584"
     CDS             7848..7934
                     /gene="uof"
                     /locus_tag="JNZ27_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY8663958.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7927..8373
                     /gene="fur"
                     /locus_tag="JNZ27_001585"
     CDS             7927..8373
                     /gene="fur"
                     /locus_tag="JNZ27_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY8663959.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8457..8783)
                     /locus_tag="JNZ27_001586"
     CDS             complement(8457..8783)
                     /locus_tag="JNZ27_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8663960.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8820..10226)
                     /gene="chiP"
                     /locus_tag="JNZ27_001587"
     CDS             complement(8820..10226)
                     /gene="chiP"
                     /locus_tag="JNZ27_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY8663961.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10805..12469)
                     /gene="glnS"
                     /locus_tag="JNZ27_001588"
     CDS             complement(10805..12469)
                     /gene="glnS"
                     /locus_tag="JNZ27_001588"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY8663962.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12672..14618)
                     /gene="nagE"
                     /locus_tag="JNZ27_001589"
     CDS             complement(12672..14618)
                     /gene="nagE"
                     /locus_tag="JNZ27_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY8663963.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKCLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14951..15751
                     /gene="nagB"
                     /locus_tag="JNZ27_001590"
     CDS             14951..15751
                     /gene="nagB"
                     /locus_tag="JNZ27_001590"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8663964.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15811..16959
                     /gene="nagA"
                     /locus_tag="JNZ27_001591"
     CDS             15811..16959
                     /gene="nagA"
                     /locus_tag="JNZ27_001591"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8663965.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16968..18188
                     /gene="nagC"
                     /locus_tag="JNZ27_001592"
                     /gene_synonym="nagR"
     CDS             16968..18188
                     /gene="nagC"
                     /locus_tag="JNZ27_001592"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY8663966.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18236..18978
                     /gene="nagD"
                     /locus_tag="JNZ27_001593"
                     /pseudo
     CDS             18236..18978
                     /gene="nagD"
                     /locus_tag="JNZ27_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19375..21039
                     /gene="asnB"
                     /locus_tag="JNZ27_001594"
     CDS             19375..21039
                     /gene="asnB"
                     /locus_tag="JNZ27_001594"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY8663967.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21419..21495
                     /locus_tag="JNZ27_001595"
     tRNA            21419..21495
                     /locus_tag="JNZ27_001595"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21453..21455,aa:Met,seq:cat)
     gene            21504..21588
                     /locus_tag="JNZ27_001596"
     tRNA            21504..21588
                     /locus_tag="JNZ27_001596"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21538..21540,aa:Leu,seq:tag)
     gene            21612..21686
                     /locus_tag="JNZ27_001597"
     tRNA            21612..21686
                     /locus_tag="JNZ27_001597"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21644..21646,aa:Gln,seq:ttg)
     gene            21721..21795
                     /locus_tag="JNZ27_001598"
     tRNA            21721..21795
                     /locus_tag="JNZ27_001598"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21753..21755,aa:Gln,seq:ttg)
     gene            21811..21887
                     /locus_tag="JNZ27_001599"
     tRNA            21811..21887
                     /locus_tag="JNZ27_001599"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21845..21847,aa:Met,seq:cat)
     gene            21936..22010
                     /locus_tag="JNZ27_001600"
     tRNA            21936..22010
                     /locus_tag="JNZ27_001600"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21968..21970,aa:Gln,seq:ctg)
     gene            complement(22164..23339)
                     /gene="ubiF"
                     /locus_tag="JNZ27_001601"
     CDS             complement(22164..23339)
                     /gene="ubiF"
                     /locus_tag="JNZ27_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY8663968.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23485..24909
                     /gene="miaB"
                     /locus_tag="JNZ27_001602"
     CDS             23485..24909
                     /gene="miaB"
                     /locus_tag="JNZ27_001602"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY8663969.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25023..26102
                     /locus_tag="JNZ27_001603"
     CDS             25023..26102
                     /locus_tag="JNZ27_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY8663970.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            26099..26566
                     /gene="ybeY"
                     /locus_tag="JNZ27_001604"
     CDS             26099..26566
                     /gene="ybeY"
                     /locus_tag="JNZ27_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY8663971.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26656..27534
                     /gene="corC"
                     /locus_tag="JNZ27_001605"
     CDS             26656..27534
                     /gene="corC"
                     /locus_tag="JNZ27_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY8663972.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27559..29097
                     /gene="lnt"
                     /locus_tag="JNZ27_001606"
     CDS             27559..29097
                     /gene="lnt"
                     /locus_tag="JNZ27_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY8663973.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29494..30401
                     /gene="gltI"
                     /locus_tag="JNZ27_001607"
                     /pseudo
     CDS             29494..30401
                     /gene="gltI"
                     /locus_tag="JNZ27_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30571..31311
                     /gene="gltJ"
                     /locus_tag="JNZ27_001608"
     CDS             30571..31311
                     /gene="gltJ"
                     /locus_tag="JNZ27_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY8663974.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31311..31985
                     /gene="gltK"
                     /locus_tag="JNZ27_001609"
     CDS             31311..31985
                     /gene="gltK"
                     /locus_tag="JNZ27_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY8663975.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31985..32710
                     /gene="gltL"
                     /locus_tag="JNZ27_001610"
     CDS             31985..32710
                     /gene="gltL"
                     /locus_tag="JNZ27_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY8663976.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32828..33763
                     /gene="rihA"
                     /locus_tag="JNZ27_001611"
     CDS             32828..33763
                     /gene="rihA"
                     /locus_tag="JNZ27_001611"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY8663977.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
BASE COUNT         8202 a         8673 c         8642 g         8271 t
ORIGIN      
        1 atgaagaacg tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg
       61 aatcacggaa agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc
      121 aactgcgccc gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt
      181 ttgactgccc gggtcattag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt
      241 tcgtggagta cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa
      301 tgggcgatct gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag
      361 tgtttaatgc cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg
      421 ttttaaacgc cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat
      481 ctaaccacca cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac
      541 gtaacccgta tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc
      601 gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg
      661 ctgtgattca gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata
      721 aaattggtca tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat
      781 ttatcccgat gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg
      841 gtattctagt tacgcaatcc gtacataaac agcaggcagg tttttctcaa acttcacaaa
      901 tccataaaaa agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga
      961 acaacgcctt tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg
     1021 atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg
     1081 taaatggcat taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg
     1141 tacctgaact ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg
     1201 atctgcgctt cttccagttt gtactagggg aacactggca ttcttttgaa ggctatgcag
     1261 agaatcaata ctttgtcgat ccttacaaac tgttgctgac aaccccaggt attgatgcac
     1321 gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg
     1381 aaaatggcgt agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg
     1441 cagaagatat ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc
     1501 ttgagtccga cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc
     1561 gctacgccgg ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc
     1621 acaacgtgaa acaactgaaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca
     1681 tgaatccgca agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc
     1741 cggatgccga aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt
     1801 gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag
     1861 aagggatcaa cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac
     1921 atgatggtcg taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc
     1981 tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt
     2041 aaccatactg acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct
     2101 tgccgaagtt gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc
     2161 actggcatgg gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg
     2221 ttatgccgag tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt
     2281 ctcactgctg attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact
     2341 gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgcagtggat
     2401 ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt
     2461 gtggggggtc attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc
     2521 gacgctttac gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc
     2581 ttccatcgct atgacgctgt gggcttttct tggtctggag tctgcgtgtg cgaatactga
     2641 tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc
     2701 ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct
     2761 ggcaaattca acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg
     2821 taaagtcatt atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt
     2881 caccattgcc caggtgttta aatcttcatc tgatgaaggc tacttcccta aaattttctc
     2941 ccgtgtaacc aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag
     3001 cgggttgccg ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa
     3061 cctggccgtg gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat
     3121 tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt
     3181 tggcgcgatg tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg
     3241 ttccatcgtg accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact
     3301 gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt
     3361 tttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc
     3421 tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct
     3481 tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgcca
     3541 atagaagcac cgttgcccgg agcagcagtc aggcgcgcgg tgatcgggtc acctgccagg
     3601 gtgctggcgc tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc
     3661 gcagctgcct gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag
     3721 tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacataatg
     3781 atgccgtcct tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg
     3841 ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa
     3901 cctaccggta cttctaccag cttacggccc aagtcattga ccacacggtc gatcatcgca
     3961 gaagaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac
     4021 aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg
     4081 ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt
     4141 gccagcagac ccgccatagc acactcggag gagcagtcca tacggatcgc accgtcttta
     4201 tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg
     4261 ttgtaatact cgccaatgcg cttccagtat tcgataccgg aaccgcccaa cggatcaacg
     4321 cccagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc cagcccttcc
     4381 acgaacggct gcaccagatc ctgctctttc acatagccgg atgccatcgc ttcgtcgagg
     4441 gagatacgct tcacgccttt caggccatcg gccatcagtg cgttggccct gtcttccacc
     4501 actttggtga cgttagtatc agccgggcca ccatttggcg gattgtactt gataccacca
     4561 tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg gccacctttt
     4621 ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc
     4681 tgcacaatga catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc
     4741 tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca
     4801 cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc
     4861 gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct
     4921 tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt
     4981 tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt
     5041 ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat
     5101 gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt
     5161 gcctggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc
     5221 cgcaaagtac acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc
     5281 gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc
     5341 tgcgtattcg tccgaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt
     5401 gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc
     5461 agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc
     5521 gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt
     5581 tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaagag tatatgaata
     5641 aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt
     5701 tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa
     5761 gaactttgcc atgaaattaa atatccgcgc gcaaactgca caaaaccagc acaataattc
     5821 tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg
     5881 cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc
     5941 aagagagccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata ccctggatgc
     6001 actgcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc
     6061 acttactgca ctggcccccg atcgcatcga taaactggtg gcgatcgata tcgcaccggt
     6121 cgactatcac gtacgccgtc atgatgagat tttcgcagct atcaacgcgg tcagtgaatc
     6181 ggacgcacaa actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg
     6241 ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta acgtaccggt
     6301 attgtgggat cagtatccac atattgtggg ttgggagaaa atcccggcat gggatcaccc
     6361 tgccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt
     6421 actggcacaa tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca
     6481 tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa
     6541 ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg
     6601 cgctgatgta ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg
     6661 aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc
     6721 acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta
     6781 ttaataaacg caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga
     6841 agctgaacgc ggaagctgtc gaggcgctga acgagctggc ggaatcgcgt aatatgagcc
     6901 gtagcgaact gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca
     6961 tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg
     7021 caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc
     7081 aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac
     7141 accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc
     7201 gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcttacga cattctgctg
     7261 ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga cttcttcccg
     7321 actctcgaag agattgattt caatggtaaa ctggttgcgc tgtttggttg tggtgaccag
     7381 gaagattacg ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg
     7441 cgcggtgcaa ccatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa
     7501 ggtctggcag atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa
     7561 ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac
     7621 gaaattctta atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat
     7681 aatgccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt
     7741 cccagtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa
     7801 tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta
     7861 tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga
     7921 ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct
     7981 cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat
     8041 ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta
     8101 ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc
     8161 gtatttgaac tgacacagca acatcaccac gatcacctga tttgcctcga ctgcggcaag
     8221 gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat
     8281 ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc
     8341 cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa aagccaacct
     8401 gcgggttggc ttttttatac aagggaaata agaaaagcga gagttacagc tctcacttat
     8461 ttgttattac tgcgtatttc ctgccagacc ttatcacaat cggctttcac cgcctgatca
     8521 tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcgtac actttcctgg
     8581 tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct ctggcaggct
     8641 tcaatttttt ctggcgaacc ttgcgcggtg ttgatacagg cgctgtatgc ctctttcaga
     8701 cggctgtctt cctgtggtgc ggttgattgc gcacaagcta ccagccccga tgccatcatg
     8761 gcgatgagaa tcagtttttt cattgaatgc ggcaggcaca aaacctgccg cgtcgggcat
     8821 cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg
     8881 ttaccgtaac cgccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc
     8941 agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg
     9001 taggcagact cttcaatagt ccggtttttg tcgttgtacg catccggatt gctctgccag
     9061 gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga
     9121 ttccagtttt tcaggtcata catcgcaccg aagaagaccg ctttttcgcc gttagcgttg
     9181 aagtaggaac ggttatccca ccagatatcc aggcggccgt tggaggaagc gtaggttggg
     9241 gtcatacgtt gcaggaagta tccctgctga ctgtcagcct taacccaggt gccttcgagg
     9301 cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag ccaagcggtg
     9361 ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgggtacc atagaactgg
     9421 tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat
     9481 tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt atttttgaag
     9541 tcgtatttcg caccgatgga gtgcagataa tcaactttgg tggttttatc gttctgataa
     9601 aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat gtaggagaaa
     9661 ctcaaggcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta
     9721 cccggcataa agctccagtg cggcgctaac agcgtttgac cggttggctg aatgtaacct
     9781 gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag gcttataccg
     9841 cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
     9901 ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc agcaatatca
     9961 aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt ccaggtggag
    10021 tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc
    10081 tgccagtaat agataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc
    10141 tccggcattg ccataaagcc cgacattgct gtaacaccgg caatagccag cgccagcgta
    10201 ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa attgctgcac
    10261 caaaggtgaa cagcgaaaaa aaataaaaat taaaaaagta gcgttgagaa aagtctcgct
    10321 gactacagga acagactatt tttcgcgacg cgaattatca atctctttct gtaaccagaa
    10381 tgtaagaaca tgagcaaccg cacatatttt gatgattttt gttacaaggc ttaagtttgc
    10441 ggttgaaagg cttgcagaag aataaaaacg tgaaattcca gcaagatgaa agcggttaca
    10501 ttttatttaa ttatgttttg tgattttaaa tttattttat ggataaattg cgaagcggaa
    10561 ttaattcgcg aagaaaagca gaaaaaacgc cgcagagcgc ggcgtttggg attgtttgct
    10621 atttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaagcacg caaattcaat
    10681 atattgcaga gatcatgtag gcctgataag cgaagcgcat caggcaattt agcgtttgaa
    10741 atttattgtc tatttattgc cggatgcgac gtaaatgcct cacccggctt ggtagaactt
    10801 aaaattactc gcctactttc gcccaggtat cacgcagccc aacggtgcgg ttaaataccg
    10861 gtttttcggc cgtggaatgg cggctgtcga ggcagaagta accttcacgc tcaaactgga
    10921 atgctttacc cgctaccgca tctttcagcg acggttcagc aaagccctgt ttgatcacca
    10981 gcgattccgg gttaatcacc gacaggaaat catccgcagc acctgggtta ggcacgctga
    11041 acagacgatc atacaaacgg atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa
    11101 taacaccttt gactttacga ccatctgccg gatctttgct taaggtgtcg gcgtcataag
    11161 tacagaagat ggtggtgata ttaccttcgg catctttctc cacgcgttca gccttaatga
    11221 cgtaagcatt acgcagacgc acttctttac ccagcaccag acgtttgtac tgcttgttag
    11281 cttcttcgcg gaaatcggcg cgatcgatcc aaatttcacc gctaaacggc acctgacggc
    11341 tccccatttc cggtttgttc ggatggttcg gcatggtgac catttcgcct tcgccctgat
    11401 agttttcgat aaccagtttc accggatcga taactgccat tgcgcgcggc gcattttcgt
    11461 tgagatcttc acggatgcag gattccagcg acgccatctc aatggtgttg tcctgcttgg
    11521 tcacgccgat gcgtttgcag aactcacgaa tagaagccgc agtgtaacca cgacgacgca
    11581 gaccggaaat ggtcggcata cgcgggtcat cccagccttc aacgtgcttg tcggtcacca
    11641 gcaggttcag cttacgcttg gacatcacag tgtattccag attcaggcgc gagaattcgt
    11701 actggcgcgg atgaacagga atggtgatgt tatccagtac ccagtcgtac agacgacggt
    11761 tgtcctggaa ctcaagcgta cacagagagt gcgtaatacc ttccagcgca tcgctgatgc
    11821 aatgggtgaa gtcgtacatc gggtagatgc accacttgtt gccagtctgg tggtgttcag
    11881 caaatttaat gcggtacagc accggatcgc gcattacgat aaatggcgac gccatgtcga
    11941 ttttcgcacg caggcaggct ttaccttctt caaaaccacc ggcacgcatt ttttcgaaca
    12001 gcgccaagtt ctcttcaacg ctgcggtcgc ggtacgggct gtttttaccc ggctgcgtca
    12061 gggtaccgcg gtattcgcgg atctgttccg gtgtcagttc atcaacgtac gccaggcctt
    12121 tattgatcag ttcgatcgca taggcgtgga gctgatcaaa ataatcggag gagtaacgga
    12181 cgttaccaga ccagtgaaaa cctaaccact ctacgtcgtt tttgatcgaa tcgacatact
    12241 cgatatcttc ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt
    12301 cctgggcgat cccgaagttc aggcagatag atttcgcatg gccaatatgc agatagccat
    12361 tcggctccgg cgggaaacgg gtgtgtactg tggtgtgctt accactggcc agatcttcat
    12421 cgatgatctg acggataaag ttagtcgggc gggcttctgc ctcactcatc gtggattcct
    12481 caaagcgtaa acaacgtata acggcgtatg atcttataag cgggacaggc tgacaactgt
    12541 tagtttttta aaattgataa tctaacaagg attatgggga tgattgatga aaaaaaaagc
    12601 ggcagagaag atctccaccg cttgtcattg ttggatgcga cgctgccgtg tcgcatcagg
    12661 cataaagcag attacttttt gatttcatac agcggtgttt gacccgccac aacatggccc
    12721 tgagctttaa tgatcaagcc actgaaatcg tcgatattgc tgcaaaccac cgggctaatc
    12781 atcgagcggg cgttagcgtt caggtaatcc agatccattt ccagaatcgg ttgccctgcg
    12841 cttacctgcg caccctcttc caccagacgt ttaaagcctt taccttccag cgctacggtg
    12901 tcgataccca tatggacgac gatctccgcg cctttttcgg tttccaggca gaacgcgtgg
    12961 ttggtgttga agattttcac gattgtccct gcggctggtg atacgacgat tttatctgtc
    13021 ggtttcaccg ccacaccgtc acccaccgct ttgctggcga atgcttcgtc aggaacctga
    13081 tccagtgcca cgacatcacc ggtaatcggc gataccagct ccgcgataga taccgcgttt
    13141 ggtacagcct gcggttttgc tacaggcgcg gcagttgccg gagttgcttc agctgacgca
    13201 gcggctaccg gaccacgggc aacgactttc ttcatcgcat cgccgatgga ttctgctttc
    13261 gcgccaacaa tcacctgaat agtctgtttg ttcagtttca ctacaccaga agcacccaga
    13321 catttacaca tcgtatcgtt gacgcgggca gagtcagcca cggtcaggcg cagacgggta
    13381 atacaggcat caatcgcttt caggttgtca gtaccgccaa ccgcagcaat atagttggtt
    13441 gccagttgag tcagaccttc ttcagtattg ctgttggctt cttcagtaac gatctcgtct
    13501 tctttatctt cacgacccgg cgttttcagg ttgaacatgc ggataaccaa actgaacacc
    13561 acgaagtaga tagcgaagaa gataacgccc atcaccagca gcatccagac gttctggctg
    13621 gcggccggca ggttatacat caacgcgtag tcgatagcac ccgcagagaa agagaagccg
    13681 gcatggatac ccagcagcgt tgccacaaac aggctgatac cggtcagcag tgcgtgcagg
    13741 aggtacagca gcggcgcaag gaacatgaac aggaattcca gcggctcagt cacaccggtc
    13801 aggaacgcag taacagcaac agaaagcagc atcccgccaa ccatcggacg acgctctttc
    13861 ggtgctgcga agtacatcgc cagcgccgca cccggcagac cgaacatcat gatcgggaag
    13921 aagccggaca tgaacatccc cgcggtgccg tcaccagcgt agaaacggtt gatgtcgccg
    13981 tggaaaaccg tacccgccgc gttggtgaat tcaccaatct ggaaccaggc gatggtgttc
    14041 agcacctgat gcagaccggt tgggatcagc agacggttga tgaaaccaaa gataccggaa
    14101 cccagcgcgc ccgcagaaac gatccactcg ccgcctgcat ggatagcgtg ctgtaccggc
    14161 ggccagacgt aaccaaaaat ggccgccagc accagacaga agaagccggt ggcgatcggc
    14221 acaaagcgtt tgccgccgaa gaagctcagg aagtccggca gtttaatatc ggaccaacgg
    14281 ttataggctg cgccaccaac cagaccggta atgatacccg ccagtacacc catgttaatt
    14341 tctgggttga tgatcaccat cgctttggtt aacacaaagt aacctaccgc acccgccagc
    14401 gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca caccgatggc gaagattaat
    14461 gcgaggttat caaaaattgc accgcccgcc tgggcaataa acgcaacgtt aagtaaatct
    14521 ggctgaccga atcgcagcaa cagcgccgcc accggcagca ccgcgatagg gagctgtaac
    14581 gccctaccga gtcgctggaa aaaacctaaa atattcatct tattccccct acgagaaccc
    14641 tatttggctc gtttaaagcc gtatttttat tttgctgcaa attttactgc cgatgttctg
    14701 taatcagatt gttagatcat ctgctacaga gtgtgtgaaa atttaattcg tatcgcaaat
    14761 taaacgcgtg tcttttgtga gttttgtcac caaatatcgt tatcatcact cccttttact
    14821 ggctaaacca gaaaacttat tttatcattc aaaaaatcaa gtcggattga cgcctgtctg
    14881 cgcaaatcca ggttacgctt aaagatgtct aatccgccaa cggcttacat tttacttatt
    14941 gaggtgaata atgagactga tccccctgac taccgctgaa caggtcggca aatgggctgc
    15001 tcgccatatc gtcaatcgta tcaatgcgtt caaaccaact gccgatcgtc cgtttgtact
    15061 gggcctgccg actggcggca cgccgatgac cacctataaa gcgttagtcg aaatgcataa
    15121 agcaggccag gtcagcttta agcacgttgt caccttcaac atggacgaat atgtcggtct
    15181 gccgaaagag catccggaaa gctactacag ctttatgcac cgtaatttct tcgatcacgt
    15241 tgatattcca gcagaaaaca tcaaccttct caacggcaat gccccggata tcgatgccga
    15301 gtgccgccag tatgaagaaa aaatccgttc ttacggaaaa attcatctgt ttatgggcgg
    15361 tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg tcttctctgg cttctcgtac
    15421 tcgtatcaaa accctgactc atgacactcg cgtcgcgaac tctcgtttct ttgataacga
    15481 tgtcaatcag gtgccaaaat atgccctgac tgtcggtgtt ggtacactgc tggatgccga
    15541 agaagtgatg attctggtgc tgggtagcca gaaagcactg gcgctgcagg ccgccgttga
    15601 aggttgcgtg aaccatatgt ggaccatcag ctgtctgcaa ctgcatccga aagcgatcat
    15661 ggtgtgcgat gaaccttcca ccatggagct gaaagttaag actttaagat atttcaatga
    15721 attagaagca gaaaatatca aaggtctgta attgttatcc ctgccctggt tccttgctca
    15781 gggcaatatt ttttaaaatc gggggtcaga atgtatgcat taacccaggg ccggatcttt
    15841 accggccacg aatttcttga tgaccacgcg gttgttatcg ctgatggcct gattaaaagc
    15901 gtctgtccgg tagcggaact gccgccagag atcgaacaac gttcactgaa cggggccatt
    15961 ctctcccccg gttttatcga tgtgcagtta aacggctgcg gcggcgtaca gtttaacgac
    16021 acagctgaag cggtcagcgt ggaaacgctg gaaatcatgc agaaagccaa tgagaaatca
    16081 ggctgtacta actatctgcc gacgcttatc accaccagcg atgagctgat gaaacagggc
    16141 gtgcgcgtta tgcgcgagta cctggcaaaa catccgaatc aggcgttagg tctgcatctg
    16201 gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata atccgaattt tgtgcgtaag
    16261 cctgatgccg cgctggtcga tttcctgtgt gaaaacgccg acgtcattac caaagtgacc
    16321 ctggcaccgg aaatggttcc tgcggaagtc atcagcaaac tggcaaatgc cgggattgtg
    16381 gtttctgccg gtcactccaa cgcgacgttg aaagaagcaa aagccggttt ccgcgcgggg
    16441 attacctttg ccacccatct gtacaacgcg atgccgtata ttaccggtcg tgaaccgggc
    16501 ctggcgggcg cgatcctcga cgaagctgac atttattgcg gtattatcgc tgatggcctg
    16561 catgttgatt acgccaacat tcgtaacgct aaacgcctta aaggcgacaa actgtgtctg
    16621 gttaccgacg ccaccgcgcc agcaggtgcc aacattgaac agttcatttt tgcgggtaaa
    16681 acaatatact accgtaacgg actttgtgtg gatgagaacg gtacgttaag cggttcatcc
    16741 ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt gcggtatcgc actggatgaa
    16801 gtgctgcgta tggcgacgct ctatccggcg cgtgcgattg acgttgaaaa acgtctcggc
    16861 acgctcgccg caggtaaagt agccaacctg accgcattca cacctaattt taaaatcacc
    16921 aagaccatcg ttaacggtaa cgaggtcgta actcaataag agaaagtatg acaccaggcg
    16981 gacaagctca gataggtaat gttgatctcg taaaacagct taacagcgcg gcggtttatc
    17041 gcctgattga ccagtacggg ccaatctcgc ggattcagat tgccgagcaa agccagcttg
    17101 cccccgccag cgtaaccaaa attacgcgtc agcttatcga acgcgggctg atcaaagaag
    17161 ttgatcagca ggcctccacc gggggccgcc gcgctatctc catcgtcacc gaaacccgca
    17221 atttccacgc aatcggcgta cggcttggtc gtcatgacgc caccatcact ctgtttgatc
    17281 tcagcagcaa agtgctggca gaagaacatt acccgctgcc ggaacgtacc cagcagacgc
    17341 tggaacatgc cctgctgaat gccattgctc agtttattga tagctaccag cgcaagctgc
    17401 gcgagctaat cgccatttcg gtgatcctgc cagggcttgt tgacccggac agcggcaaaa
    17461 ttcattacat gccgcatatt caggtagaaa actgggggct ggtagaagca ctgaaagaac
    17521 gttttaaagt gacctgtttc gttggtcacg atatccgtag tctggcgctg gcggagcact
    17581 acttcggtgc aagtcaggat tgcgaagact ccattctggt gcgtgtccat cgcggaaccg
    17641 gggccgggat tatctctaac gggcgcattt ttattggccg caacggtaac gtcggtgaaa
    17701 ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca ctgcggcaac tttggctgcc
    17761 tggaaactat cgccgccaac gctgccattg aacaacgggt gttgaatctg ttaaagcagg
    17821 gctaccagag ccgtgtgcca ctggacgact gcaccatcaa aactatctgc aaagccgcga
    17881 acaaaggcga tagcctggcc tcggaagtga ttgagtatgt cggtcgtcat ctgggtaaaa
    17941 ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat tgttattgct ggtgaaatca
    18001 ccgaagccga taaagtgctg ctccctgcta ttgaaagctg cattaatacc caagcgctga
    18061 aggcgtttcg tactaatctg ccggtggtac gttctgagct ggatcaccgc tcggcaatcg
    18121 gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat tttgctccag catttgctgg
    18181 aaaattaatg tgcttttata gtggcgctta ttgttgtcaa tattctgggt agtccatgac
    18241 cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg cacgataacg tcgccgtacc
    18301 gggtgcagcg gaatttttgc acgggattat ggataaaggc ctgccgctgg tgttgctgac
    18361 caactatcct tcgcagactg ggcaagatct ggcgaaccgc tttgccactg ccggtgtcga
    18421 tgtgccggat agcgtgtttt atacctctgc gatggcgacc gccgatttcc tgcgtcgcca
    18481 ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg atttatgaac tgtacaaagc
    18541 cggtttcact attaccgatg tgaaccctaa tttcgtgatt gttggcgaaa cacgttccta
    18601 caactgggac atgatgcata aagcagccta tttcgtcgct aacggtgcac gctttatcgc
    18661 caccaacccg gacacggttt ttatcccgct tgtggcgcgt tgtgtgcagg gattgagaaa
    18721 atctccgggc gcaaaccgtt ctatgttggt aagcccagcc cgtggatcat ccgcgcggca
    18781 ttaaacaaaa tgcaggcgca ttcggaagaa acagtgattg tcggcgataa cctgcgtacc
    18841 gatattctgg cgggcttcca ggccggtctg gagacgattc tggtgctttc tggtgtttca
    18901 tcgctcgacg atatcgacag tatgccgttc cgcccaagct ggatttaccc gtcagtcgct
    18961 gaaatcgacg ttatctgaat accacccacg tctgcggacg tggttggctc tgatgacaaa
    19021 cgccaaactg cctgatgcgc tacgcttatc aggcctacgc agctcctgca atatattgaa
    19081 ttttcatgct tttgtaggcc gtataaggcg ttcacgccgc atccggcatg aacaaatcgc
    19141 actttatcag caatttggcc cccgtaaaat ataaccattc aataaaaccc gctaaaaatt
    19201 atcgattcat caataaatac atcctcacac cagacccttt ttcaccaatc gacaatcacc
    19261 attacgtttt ttatttttgc actgccaaat agcttgcgca cagtgctgct ttgcggcatt
    19321 tttttaaaca agcaaacaca acaagcaaca aataccaggt taacggagaa ggttatgtgt
    19381 tcaatttttg gcgtattcga tatcaaaaca gacgcagttg agctgcgtaa gaaagcactc
    19441 gagctgtcac gcctgatgcg tcatcgtggc ccggactggt ccggtattta tgccagcgat
    19501 aacgccattc tcgctcacga acgtctgtca attgttgacg ttaacgcggg ggcgcaacct
    19561 ctctacaacc aacaaaaaac ccacgtactg gcggtaaacg gtgaaatcta caaccaccag
    19621 gcattgcgcg ctgaatatgg cgatcgttat cagttccaga cagggtctga ctgtgaagtg
    19681 atcctcgcgc tgtatcagga aaaagggccg gaatttctcg acgacttgca gggcatgttt
    19741 gcctttgccc tgtacgacag cgaaaaagat gcctacctga ttggtcgcga ccatctgggg
    19801 atcatcccac tgtatatggg gtatgacgaa cacggtcagc tgtatgtggc ctcagaaatg
    19861 aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc cggcggggag ctatttgtgg
    19921 agtcaggacg gcgaaatccg ttcttattat catcgcgact ggttcgactt tgatgcagtg
    19981 aaagataacg tgaccgacaa aaacgagctg cgtcaggcac tggaagattc cgttaaaagc
    20041 catctgatgt ctgatgtgcc ttacggtgtg ctactttctg gtggtctgga ttcctcaatt
    20101 atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg aagatcagga acgctctgaa
    20161 gcctggtggc cgcagttaca ctcctttgct gtaggtctgc cgggttcacc ggatcttaaa
    20221 gcagcccagg aagtggcaaa ccatctgggc acggtgcatc acgaaattca cttcactgta
    20281 caggaaggtc tggatgccat ccgcgacgtg atttaccaca tcgaaactta tgatgtgacc
    20341 actattcgcg cttcaacacc gatgtattta atgtcgcgta agatcaaggc gatgggcatt
    20401 aaaatggtgc tgtccggtga aggttctgat gaagtgtttg gcggttatct ttacttccat
    20461 aaagcgccca acgctaaaga actgcatgaa gagacggtgc gtaaactgct ggccctgcat
    20521 atgtatgact gcgcgcgcgc caacaaagcg atgtcagctt ggggcgtgga agcacgcgtt
    20581 ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgca ttaacccgca ggataaaatg
    20641 tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat gttttgagtc atacctgccc
    20701 gcaagcgtgg cctggcggca gaaagagcag ttctccgatg gcgtcggtta cagttggatc
    20761 gacaccctga aagaagtggc ggcgcagcag gtttctgatc agcaactgga aactgcccgc
    20821 ttccgcttcc cgtacaacac gccgacctca aaagaagcgt atctgtaccg cgagatcttt
    20881 gaagaactgt tcccacttcc gagtgccgct gagtgcgtgc cgggcggtcc ttccgtcgcg
    20941 tgttcttcag ctaaagcgat cgaatgggat gaagcgttca aaaaaatgga cgatccgtct
    21001 ggtcgtgcgg ttggcgttca ccagtcggcg tataagtaag aaatgcggta ctgccccgga
    21061 gaaatccggg gctgttttag ttattgcgct gcttgttgat tctgatatgc agtaaggaaa
    21121 tgttccgggt ttgccacatc actgtttgac aactcatcac tgtaggccag tatttcatag
    21181 attaatttcc caccactatt gttgaattcc gaatttcatc ctgtacgaca aaagaatggc
    21241 tgtagggtgg ggaaaaaaca caaagtaaac aataattgac gaatatagcg ccacgctgtt
    21301 cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg ttgacgctgc
    21361 aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa aaaaagatgg
    21421 ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca ggttcgaatc
    21481 ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca ccagatttag
    21541 gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt caccagaaag
    21601 cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc
    21661 tggttcgaat ccaggtaccc cagccatctt cttcgagtaa gcggttcacc gcccggttat
    21721 tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct ggttcgaatc
    21781 caggtacccc agccatcgaa gaaataatct ggctacgtag ctcagttggt tagagcacat
    21841 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat tctgaatgtg
    21901 tcgaatacgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag cggtaaggca
    21961 ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca cattaaaaaa
    22021 gctcgcttcg gcgagctttt tgcttttctg cgttcattca atgtcgaatg cgatgtcgac
    22081 acgtcttatc cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc tacggttggc
    22141 acgcatccgg caatgttgta aggctacaac cctaacgcat atttcagcgc ctgacgtttc
    22201 aacacgccag cacgctctgc cgccattaac ccgagattac gcacaaaacg cagcggcggc
    22261 agattattac tgaatccggc ataaaacaga tccataccac tttgcataat gaagttatcc
    22321 gccatgcgcc gcatctggta acgcttgagg acaggataac tggcccacgc ttcaccgtag
    22381 ctgcgggcat tcaccagaac atcaatcagg gcatcgacat cacgataacc aagattcacc
    22441 ccctgccccg ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc aagccccggc
    22501 tgcacatact gcaacgcatg tcggcgcgtc agcggaaacg caccagcggc aagcggtgta
    22561 acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg cctggagttg cgccatattc
    22621 atattctgca actggcgaat acgcgccgga gagtcatacc acaccagcga tgcccagtta
    22681 tcaaacaacg gcagaaacgc acgcggtccg tccggggtaa attgctgcca ggtgctgtcg
    22741 ccgggatcgt tctcacactg tacgctaatc aacatgcacg actgcgcata ctgccaggca
    22801 tgaacgccaa ttcccgccat ctgccgcacc tgagaatttg cgccgtcggc accaatcacc
    22861 agtttcgcgc ggatcgtttc accgcccttc agttccagct cctgaagatc attatctcga
    22921 tgtaatgcaa tcagcgaggt tggcacacgt aacgttactt tcggatgcgc ttccagcgcc
    22981 tgccacaacg cctgttgcag gactgtgttt tccaccatgt agccaagcaa cggcaacttt
    23041 aattcggctg cgtcaaatac cacatgtgcc gtttcccact cccaggtttc cagtctgcgg
    23101 taaggatggc aacgcatagc ctgtactgca tcccagaccc ctaccccttt aagcaatgat
    23161 accgaagccg cgctgatcgc cgagatccgc acgtccggtt ggctgtcagc gacaaacggt
    23221 gcaggttctg cgtgctcgat caccgttacc gaaaatccgt gctgtgccag ccccagcgcc
    23281 agtgcgccgc cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg
    23341 gtcaatcatc ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga
    23401 taacgctcat actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg
    23461 ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt
    23521 cagatgaacg agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat
    23581 caactgaccg acgtggcgga agaagcggat gtgctactgc tgaacacctg ctcaatccgc
    23641 gagaaggctc aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag
    23701 aatccagacc tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt
    23761 cgccagcgcg cccactatgt cgatattatt tttgggccgc aaacgctgca ccgtctgccg
    23821 gagatgatca actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa
    23881 atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc
    23941 atcatggaag gctgcaataa atattgcacc tactgcgtgg tgccttacac ccgtggtgaa
    24001 gaggtaagcc gtccgtctga cgatattctg tttgagattg cccagcttgc ggctcagggc
    24061 gtgcgtgaag tcaacctgct cggtcagaat gtgaatgcct ggcgtggtga gaactacgac
    24121 ggcaccaccg gatcgtttgc cgatctgttg cgtctggttg ctgcgatcga cgggatcgat
    24181 cgtattcgct ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtac
    24241 cgcgacacgc ctgagctggt gagcttcctg catctgccgg tacagagcgg ttccgatcgc
    24301 attctgaacc tgatgggacg tacccatacg gcgctggagt acaaagcgat catccgtaaa
    24361 ctgcgtgcgg cgcgtccgga tattcagatc agttctgact tcattgttgg cttccctggc
    24421 gaaaccaccg aagacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg
    24481 agctacagct ttatcttctc tgcacgtcca ggaacgcctg ccgccgatat ggttgatgat
    24541 gttccggaag aagagaagaa gcagcgtctg tatattctgc aagaacgtat caatcagcaa
    24601 gcgatggcat ggagccgtcg gatgctcggc accacccagc gtattctggt agaagggact
    24661 tcgcgtaaga gcatcatgga gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc
    24721 gagggcacac cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctacccg
    24781 aactctctgc gcggtaaagt ggtgcgaact gaagatgaaa tgggtctgcg catggcagaa
    24841 acaccggagt cggtgattgc ccgcacccgc aaagaaaacg accttggcgt gggttattat
    24901 cagccgtaat tctcaggcct gccgttccgg tgggccttgt tttttctctc tttatcccca
    24961 tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt
    25021 gaatgttcag cttccggcgg catacagata agaggaacgg tttgaacata gacactcgcg
    25081 aaatcaccct ggagccagca gacaatgcgc gtctgttgag cctgtgcggc ccgtttgatg
    25141 acaacatcaa gcagctcgaa cgccgtctcg gcatcgagat caatcgccgc gataaccact
    25201 ttaaactgac cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt
    25261 atgtcgatac tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc
    25321 ttgcgattaa agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg gagtacggca
    25381 aagcggtcaa tatcaaaact aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc
    25441 agtacatcgc caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg
    25501 gtaaaaccta cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gaaattcgcc
    25561 gtattctgct gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg
    25621 atttaagcca gaaagtagac ccgtatctgc gcccactgta cgacgcgctg tttgaaatgc
    25681 tgggctttga gaaagtcgag aaactgattg agcgcaacgt tattgaagtc gcgccgctgg
    25741 cctatatgcg tggtcgtacg ctgaacgacg cgtttatcat tctcgatgag agccagaaca
    25801 ctaccatcga acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta
    25861 tcaccggcga cgtcacacag atcgacctgc cgcgtaatac taaatcaggc ttacgtcatg
    25921 ccattgaagt gctggccgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg
    25981 tggttcgtca cccggtggtg gcgcgtatcg ttaacgctta tgaagccagg gaagaagcag
    26041 aacaaaaacg taaagcggcg ctggcagcag aacgcaagcg cgaagaacag gaacaaaaat
    26101 gagtcaggtg atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga
    26161 gagccagttt caaacatggc tgaatgcggt gatcccgcag tttcaggaag agtcggaagt
    26221 gactattcgc gtggtcgata ccgccgaaag ccacagtctg aatctgacct accgcggtaa
    26281 ggataagccg accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggagatgtc
    26341 gctactgggc gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg
    26401 caaaccactg gaggcgcact gggcgcatat ggtggtgcac ggcagtctgc atttgttagg
    26461 ttacgatcac attgaagatg acgaagcaga agaaatggaa gccctcgaaa cagagattat
    26521 gcttgctctg ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt
    26581 gactggcaga cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag
    26641 aacccatacg acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag
    26701 aagggatttt tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac
    26761 gaactgctgg cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc
    26821 gatatgctcg aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc
    26881 cgctcccaga tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc
    26941 atcgagtccg cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg
    27001 attctgatgg cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcggcatg
    27061 gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg
    27121 aaagagtttc gctctcagcg ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt
    27181 tccggtctgg tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag
    27241 tatgacgaag aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc
    27301 gcactggctt ccattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagaa
    27361 gtcgacacta tcggtggtct ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa
    27421 actatcgaca tcgacggtta ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt
    27481 caggttcatg tcaaaatccc ggatgactca ccccagccaa agctggatga ataaaaccga
    27541 aactggatag ataactacat ggcttttgcc tcattaattg aacgccagcg cattcgcctg
    27601 ctgctggcgt tattattcgg tgcctgcgga acgctggcct tctctcctta cgacgtctgg
    27661 cctgcggcga ttatttcgct gatggggctt caggcgttga cctttaaccg tcgcccactc
    27721 cagtctgccg ctattggctt ttgctgggga tttggcctct ttggcagcgg tattaactgg
    27781 gtctatgtca gcatcgcgac ctttggcggg atgcctggcc cggttaacat cttcctggtg
    27841 gtgctgctgg cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgt
    27901 ctgtggccga aaaccacctg gctgcgcgtg gcgattgccg cccctgctct ctggcaagtg
    27961 accgagtttc tgcgcggttg ggtgctgact ggcttcccgt ggttacagtt cggctatagc
    28021 cagattgatg gcccgttaaa agggctggca ccgctaatgg gcgtggaagc cattaacttc
    28081 ctgctgatga tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa ctggcgtccg
    28141 ctggtggtgg ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat ccaatggttt
    28201 accccgcaac cggagaaaac cattcaggtt tcgatggttc agggcgatat tccgcaatcg
    28261 ctgaaatggg acgaaggcca gcttcttaat acgctaaaga tttactacaa cgcaacggca
    28321 ccgctgatgg gcaaatcatc gttgattatc tggccggagt cggcgataac cgatctggaa
    28381 attaatcagc aaccgttcct caaagcactg gacggtgagt tgcgtgataa aggtagctcg
    28441 ctggtaaccg ggattgtcga cgcgcgtctc aataagcaga accgctacga tacctacaac
    28501 accatcatca cgctgggtaa aggtgcaccg tacagctacg aatcagccga tcgctataac
    28561 aaaaaccatc tggtgccgtt tggcgagttt gtcccgctgg agtcgattct gcgtccgtta
    28621 gcaccgttct ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg
    28681 ctgtcggcaa atggtattga gcttactgcg gctatttgct acgagatcat tctcggcgag
    28741 caagtgcgcg ataacttccg cccggatacc gactacctgc tgactatctc caacgatgcg
    28801 tggtttggta aatctattgg tccatggcaa cacttccaga tggcgcgaat gcgtgcgctg
    28861 gagctggcgc gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg
    28921 cagggtgaga ttcaggcgat gatcccgcag ttcacccgcg aggtgttaac cactaacgtg
    28981 acgccgacca ccggactcac accatacgca cgtaccggca actggccgct gtgggtgctg
    29041 acggcactgt ttggttttgc tgctgtgttg atgagtctgc gtcagcgacg taaataatcc
    29101 cttcttatcg tgccggaata catcccatat ttcggcacgc tcaccggatg cggcatcagt
    29161 gcattatccg gataacaata ttctcctatc caactttggc acatctattg ctttgttata
    29221 caaggcaaag cttgaaccag catcagtaca acccggttgc accacagcaa tccaccggta
    29281 gcaccaaaag agtgcatttt aagaattttg cttcagaatg gtgcggtgca cctcgcaaaa
    29341 acaaacaata aaacatcaat atctttacat ttagccaaac taaatgttac ctaacaggca
    29401 caacactgca caataaagtt gcagacgata acaacacaaa cactcacaac gggtatccat
    29461 gcgttcttaa cgcagaagat aaaggagttg gatatgcaat tacgtaaacc tgccacagca
    29521 atcctcgccc tggcgctttc cgcaggactg gcacaggcag atgacgccgc cccggcagca
    29581 ggcagtacgc tggacaaaat cgccaaaaac ggtgtgattg tcgtcggtca ccgtgaatct
    29641 tcagtacctt tctcttatta cgacaatcag caaaaagtgg tgggttactc gcaggattac
    29701 tccaacgcca ttgttgaagc agtgaaaaag aaactcaaca aaccggactt gcaggtaaaa
    29761 ctgattccga ttacctcaca aaccgtattc cactgctgca aaacggcact ttcgattttg
    29821 aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc tctgacacta
    29881 ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa gattttgccg
    29941 acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt ttgctcaaca
    30001 aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat cacggtgact
    30061 ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac gctctgctgg
    30121 ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc aagccgcagt
    30181 ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa aagctgatgg
    30241 atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt gataaatggt
    30301 tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca gacgaaatga
    30361 aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa ccaggggcgg
    30421 aaattctagc cctctcgatt gttacgtagc acggacagac tatacgcctg atggtcgttc
    30481 cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta gcagttaacg
    30541 ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg gggtattttt
    30601 ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag tggttttcag
    30661 gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg ttcatttttc
    30721 ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt gtatgtcgaa
    30781 ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt gatcccggag
    30841 ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa tattcagttt
    30901 ttcctttcat ccatgctctg cctggggctg tttaccgcct cccgtgtttg cgaacaggta
    30961 cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc gatgggcctg
    31021 acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt tatcgtcccg
    31081 ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc gaccatcggt
    31141 ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca cgcctgggag
    31201 tcgtttaccg ccatcacgct tacgtatgtg ttgattaacg cttttatcat gctggtgatg
    31261 acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata atgtacgagt
    31321 ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg gtgatcaccc
    31381 tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg ctggcggtga
    31441 tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt aacgtattcc
    31501 gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg ggatttctgc
    31561 aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg atggtggcgt
    31621 tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt caaagtattt
    31681 cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag tcgatgaaac
    31741 tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact cagggcatcg
    31801 tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc ttccgtaccg
    31861 cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt gccggatttg
    31921 tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa agaaggacag
    31981 cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg tgctgaccga
    32041 ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt ctggttccgg
    32101 caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag gtgaaatcac
    32161 cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc gctcccgcgt
    32221 cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg aaaacctgac
    32281 cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa aagcattgaa
    32341 attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc agctttccgg
    32401 cggtcagcag cagcgtgtgg caatcgctcg cgcattgtgt atggctccta ttgcgatgct
    32461 gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc tggacgtgat
    32521 ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa tgggctttgc
    32581 ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg aagactcgcc
    32641 gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc tcgcgaaaat
    32701 cctgcattaa tcactctggc gcgcataccc aattgcgcgc catctcacgc tttatcgttt
    32761 cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca cacaacaagg
    32821 agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg acgctatcgc
    32881 aatagttctc gccctcgcct caccagagct tgatgtcaaa gcaattacgt cttccgccgg
    32941 aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct tgcttaatcg
    33001 caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt tgattatcgc
    33061 ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac cgacgttcgc
    33121 accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa gtgcggaacc
    33181 tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca atagccatcc
    33241 ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg ggcttggtaa
    33301 ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag aaattgtctt
    33361 ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag cgcaaatcca
    33421 cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca ttgttgccga
    33481 actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg tcggcgcacc
    33541 gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta ccactgttga
    33601 gcgctgggtt ggcgtggaaa cacaggggaa atataccctg ggtatgacgg ttgttgatta
    33661 ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg atcgtcaggg
    33721 ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt cattgattaa
    33781 gatcacca
//
LOCUS       DADMFL010000034        33442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.034, whole genome shotgun sequence.
ACCESSION   DADMFL010000034 DADMFL010000000
VERSION     DADMFL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.034"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="JNZ27_001612"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="JNZ27_001612"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY8663978.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /locus_tag="JNZ27_001613"
     CDS             complement(2992..3921)
                     /locus_tag="JNZ27_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY8663979.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="JNZ27_001614"
     CDS             complement(4138..4374)
                     /locus_tag="JNZ27_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8663980.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JNZ27_001615"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JNZ27_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY8663981.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGKSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTIPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JNZ27_001616"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JNZ27_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY8663982.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGHGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JNZ27_001617"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JNZ27_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY8663983.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JNZ27_001618"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JNZ27_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY8663984.1"
                     /translation="MNNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JNZ27_001619"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JNZ27_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY8663985.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB"
                     /locus_tag="JNZ27_001620"
     CDS             complement(10720..11346)
                     /gene="ipgB"
                     /locus_tag="JNZ27_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY8663986.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JNZ27_001621"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JNZ27_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY8663987.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JNZ27_001622"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JNZ27_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY8663988.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="JNZ27_001623"
     CDS             13562..15178
                     /locus_tag="JNZ27_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY8663989.1"
                     /translation="MHITNLGLHQVSYQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="JNZ27_001624"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="JNZ27_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY8663990.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="JNZ27_001625"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="JNZ27_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY8663991.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16017..17123
                     /gene="mxiG"
                     /locus_tag="JNZ27_001626"
     CDS             16017..17123
                     /gene="mxiG"
                     /locus_tag="JNZ27_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY8663992.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEKIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFFR"
     gene            17141..17392
                     /gene="mxiH"
                     /locus_tag="JNZ27_001627"
     CDS             17141..17392
                     /gene="mxiH"
                     /locus_tag="JNZ27_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY8663993.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17405..17698
                     /gene="mxiI"
                     /locus_tag="JNZ27_001628"
     CDS             17405..17698
                     /gene="mxiI"
                     /locus_tag="JNZ27_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY8663994.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17704..18429
                     /locus_tag="JNZ27_001629"
     CDS             17704..18429
                     /locus_tag="JNZ27_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY8663995.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18426..18953
                     /locus_tag="JNZ27_001630"
     CDS             18426..18953
                     /locus_tag="JNZ27_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY8663996.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18895..19590
                     /locus_tag="JNZ27_001631"
     CDS             18895..19590
                     /locus_tag="JNZ27_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY8663997.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19583..19990
                     /locus_tag="JNZ27_001632"
     CDS             19583..19990
                     /locus_tag="JNZ27_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY8663998.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLVSVFEAGREADDSTW"
     gene            19974..20402
                     /gene="mxiM"
                     /locus_tag="JNZ27_001633"
     CDS             19974..20402
                     /gene="mxiM"
                     /locus_tag="JNZ27_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY8663999.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20532..21164
                     /gene="mxiE"
                     /locus_tag="JNZ27_001634"
     CDS             20532..21164
                     /gene="mxiE"
                     /locus_tag="JNZ27_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY8664000.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21151..22851
                     /gene="mxiD"
                     /locus_tag="JNZ27_001635"
     CDS             21151..22851
                     /gene="mxiD"
                     /locus_tag="JNZ27_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY8664001.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22870..23937
                     /gene="mxiC"
                     /locus_tag="JNZ27_001636"
     CDS             22870..23937
                     /gene="mxiC"
                     /locus_tag="JNZ27_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY8664002.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23950..26010
                     /gene="mxiA"
                     /locus_tag="JNZ27_001637"
                     /gene_synonym="virH"
     CDS             23950..26010
                     /gene="mxiA"
                     /locus_tag="JNZ27_001637"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY8664003.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26023..26424
                     /gene="spaK"
                     /locus_tag="JNZ27_001638"
     CDS             26023..26424
                     /gene="spaK"
                     /locus_tag="JNZ27_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY8664004.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26428..27720
                     /locus_tag="JNZ27_001639"
     CDS             26428..27720
                     /locus_tag="JNZ27_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY8664005.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27813..28151
                     /gene="spaM"
                     /locus_tag="JNZ27_001640"
     CDS             27813..28151
                     /gene="spaM"
                     /locus_tag="JNZ27_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY8664006.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28138..29016
                     /gene="spa32"
                     /locus_tag="JNZ27_001641"
     CDS             28138..29016
                     /gene="spa32"
                     /locus_tag="JNZ27_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY8664007.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29010..29891
                     /gene="spaO"
                     /locus_tag="JNZ27_001642"
     CDS             29010..29891
                     /gene="spaO"
                     /locus_tag="JNZ27_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY8664008.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29896..30546
                     /gene="spaP"
                     /locus_tag="JNZ27_001643"
     CDS             29896..30546
                     /gene="spaP"
                     /locus_tag="JNZ27_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY8664009.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30561..30821
                     /gene="spaQ"
                     /locus_tag="JNZ27_001644"
     CDS             30561..30821
                     /gene="spaQ"
                     /locus_tag="JNZ27_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY8664010.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30821..31591
                     /gene="spaR"
                     /locus_tag="JNZ27_001645"
     CDS             30821..31591
                     /gene="spaR"
                     /locus_tag="JNZ27_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY8664011.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31598..32626
                     /gene="spaS"
                     /locus_tag="JNZ27_001646"
     CDS             31598..32626
                     /gene="spaS"
                     /locus_tag="JNZ27_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY8664012.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32639..32881
                     /locus_tag="JNZ27_001647"
     CDS             32639..32881
                     /locus_tag="JNZ27_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664013.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10450 a         4850 c         6691 g        11451 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccctgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc tggtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctaca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atattttagt cactcgttgt aattggagta tgctttattt tatttgaaaa
     2341 tagatgtatt ttatacccgc aatttaaact gtaagcccta tgaggggtat ggatattata
     2401 agttactctt tgactaagca taacttttgg ggttagaaaa ataaatctct tgttaataat
     2461 taatatgatt ttaaaatcaa acttgtttat aattataaaa taatgtaagt cggtcatttt
     2521 atgggaagat taggagaata aaacagtaat tatatttgag aaatctgcta atatattctt
     2581 aaagcttttt tgatactgtg aacgaagtct gttaatggtg gattttttgt ttatcaccat
     2641 gtaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattggcaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgcagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttgtgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttattttat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc
     4681 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt atttatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg gggatggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgactttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca attaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgccat gtccacctag attatctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatactga caacatatcc cccgtttttt tgagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagttcttcc aatgcctttt taagcgagtt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat
     6901 atcatttgat ctccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatattc gttcctggaa agaatatcca atagttgagc ggatttattt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg aaagctcttt ctttaatgcc ttatttgttg ttctgatatt atgaagggtg
     7141 tcgttaagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gccgaattcc
     7381 ttgattgggt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtttcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7621 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7741 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tttattttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8161 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct
     8401 gggttggttt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8641 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9661 tttttctttt ctggtgactc tggggcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtgt taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgctgg gacttccaag cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10021 tgtgcattag ttgtattaat attttggtta gcagtaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcttgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggaaa accagtggtt gtggtgctta cattattcat aataattact
    10201 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttagcttt taagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aatactggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcagtaa tatttaaaga cataaggtct cctttttgtc
    10681 tgctttactt taataagtat aagatttaat ataaaagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tatagcgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataaga
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgtgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcat acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaagaat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgatctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caataatttt
    11881 tattgatttt tcgataattt cttctttttt atctgttgct tcggttttta attcgtttaa
    11941 tacacgagtc atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cacatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttggtg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatttc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 atcgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt ctttttgcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgc ttataggtat cgacactgta acctaagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat ccccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact agtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaaaacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 acgctctgcg gtattcaaca ttaatttgat gtctctacct gtgaaagagt gttgaattga
    12961 gtttttagct gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 attgaggaaa agcaataagg atttttgatt ctctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta tcaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg aggctcatac tttattaact
    13261 ctccattact tggtgattgt ggggatgtaa aatgtatttg gcatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctcgttt catcatgaaa tcccacaaga taaagtgcct
    13441 gatgtatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcatat caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagaa agaaataagg ggatcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga gctgaagttt tttacgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac gattagatac
    13861 ctataaactt ggtgaagtta ataaaagaca tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaaacga aaataaaaca
    13981 aatatttact gattatttaa ataataagaa ttggggaccg gtcgacaaga atattagtca
    14041 tcatgggaaa aattatggtt ttcaattaac acctgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 tgagcatatc gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
    14341 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
    14461 cgcattaaaa gggttaaact ctaaaagggg gaagccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attataaaca agaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 tcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
    15121 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
    15241 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgctta gctactgatg atgaaggcgg
    15421 aaatctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggatg tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt ttatcccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaatattc catcaagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tctgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tattctgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgatgat gtatggaaga gggtgggagg ctgttggagc ctataatgca
    15901 ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctatagacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gacaatttcc cagaaaatat tattcctgta acagatagta
    16201 aatcggatgg tataatttat ttgaccataa gcaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga gaaaattgat atcaattcac aatttaattc atttgaatat gacgggattt
    16321 cttttcacct taaaaatatg agggaggata aatcgagagg ccatatacta aacggaatgt
    16381 ataagaacca ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacactgga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcgtc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 caaacaaaga aataaataga attcaacaat atatcaatca acgattccca tttatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtgttttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgata aattgaaaaa tgctctgatt gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg ggggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaaat aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgaacataa aaaaatatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgaa
    17161 tgatgattgg acattgagtt cattatctga aacttttgat gatggaactc aaacattaca
    17221 aggtgaacta acattggcac tagataaatt agctagaaat ccttcgaatc cacagttgct
    17281 ggctgaatac caaagtaaat tatctgaata tacattatat aggaacgcgc aatccaatac
    17341 agtgaaagtg attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc
    17401 attcatgaat tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca
    17461 atctcaagag atatcaagtc tggaagacgt cgtgtcggct aaatatagtg atattaagat
    17521 ggatacagat attcaagtat cacaaataat ggagatggta agcaatccag aatcattaaa
    17581 cccagaatct ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt
    17641 agctggcacg ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt
    17701 tatatgatta ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag
    17761 caacgtgaag agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg
    17821 ctagaacgcc ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta
    17881 caagtcgaaa aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca
    17941 aatccggaga gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca
    18001 agagctgaaa aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt
    18061 tctattggtg gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat
    18121 atatcttcaa aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct
    18181 gaactattag ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat
    18241 gaaaatatat ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct
    18301 gttaaggaag ttaaatcgga atttttaaca aatgaagtaa tatatttatt tctcgggatg
    18361 gctgtactag ttgtcattct tttggtatgg gcattcaaaa cagggtggtt caagagaaac
    18421 aaaatatgat aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag
    18481 cgttctatat aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa
    18541 tcagaagtga gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac
    18601 ctttaagcgg ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt
    18661 attttatcgg tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt
    18721 atgggggcaa aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta
    18781 ttgcgtggaa ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc
    18841 caattgcttt ggagggaaat tatacgcctg tagaaagaaa tttgtcccga ttaaatgaag
    18901 gtatgcaata tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt
    18961 gttataaaga gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt
    19021 aagaagaaag caatttgtgt tataaaggag gctacgaaaa aagctgaatc actgcgtata
    19081 gatgctgtat gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac
    19141 tatatttgtg aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata
    19201 actagtttgc tttcagaaaa tttacatgat gagaggatca ttagcacttt attggagcag
    19261 tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc caagtgtaat
    19321 ttagcactga gtaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt
    19381 attttaaaat attctgaagg gaataattat atattctgta gtggtaatca agtagtggag
    19441 tttagtcctc aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa
    19501 aatgataaaa aatatttcaa agagttagca cataagaaac tgagacaaat tgcagaagac
    19561 ttgttaaaag agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca
    19621 aagattaaat agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt
    19681 tgatgaggat ataatctatg aaataatgca atatttctca cagagtgaat taaattccat
    19741 agataatgat gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct
    19801 tacagctgca caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga
    19861 tttgcatgag ggattttatg catcattgtc catatataac aatattgatt tctatattaa
    19921 gactacaacc ttcgattcat tagttagtgt ctttgaagca gggagagagg cagatgattc
    19981 gacatggtag taataagttg aaaatattta ttttaagtat attgctatta acactgagtg
    20041 ggtgtgcttt aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta
    20101 aggattattt ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta
    20161 taaatgttta ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat
    20221 tcatgggcaa ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag
    20281 ctatagataa actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa
    20341 atgataccat ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat
    20401 aattgtattg tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct
    20461 agtggacatg aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataattgag
    20521 ctggctccag catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata
    20581 atgtgaacta tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg
    20641 actatttcag tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc
    20701 atgagaatga tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa
    20761 gattcatctt ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg
    20821 agtcatatca tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa
    20881 taaagagtct gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa
    20941 aagctttagg ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat
    21001 tagatgtatt tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat
    21061 caacatccca tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat
    21121 cgaatattac ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact
    21181 ctcttgattg tattgttacc attgatagtt aatgctaata atattgactc tcatttatta
    21241 gaacaaaatg acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgaa
    21301 cgtttttctg cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga
    21361 atatctgggg agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt
    21421 gtgggactca tatggtataa agatggaaat gcattataca tatatgactc tggagaatta
    21481 ataagcaaag ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag
    21541 gatgctaacc tttatgatca cagatatcca ataagaggaa acattagtga taaaacattc
    21601 tatatatctg gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat
    21661 aagcaggttt cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat
    21721 acatttgtta gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg
    21781 gctactgttg tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagcccaa
    21841 aatgatccga tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt
    21901 tcatttagtt cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca
    21961 gaaaccaatt ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt
    22021 atcactcagt tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt
    22081 gataaatctg aattaaataa ccttggtgtt aattggcaag gaacggcttc atttggtgat
    22141 tcgtttggtg cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat
    22201 aagtttattg cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg
    22261 ccggtgattt tgacacaaga gaatattccg gctatatttg ataataacag gactttttat
    22321 gtgtcattag ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata
    22381 aatgttatac ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat
    22441 ggaacaggaa atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca
    22501 gaagttggaa ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg
    22561 ataggtggtt atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc
    22621 tctattcctg taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta
    22681 agggtttttc tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa
    22741 tataaaagcc tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa
    22801 actactttgc tggaagacga aaaatcattg gtttcatact taaattacta actataaagt
    22861 aggtgatgta tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag
    22921 ttgaatgcaa tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct
    22981 ggcttggcta atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca
    23041 tttataaaca gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt
    23101 attttagatg gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacgtta
    23161 aaagataacc ttcctctaga tcgggatttt atagacagac taaagagata ttttaaagat
    23221 ccaagtgatc aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa
    23281 caagtcgaat tattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt
    23341 aatgctggaa taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa
    23401 ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat
    23461 cagtatatag aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt
    23521 gtagagcagt cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag
    23581 tttggttttg tgttatctaa attaattgca attaagatga ttagaacttc agacgtaatt
    23641 tttatgaaga aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag
    23701 ctattgctaa ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact
    23761 tctgttatag aagtatcaca agccagtcat gaggattctg tagtgtatca aacatatcta
    23821 tcttctgtta atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg
    23881 atcaatattc tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag
    23941 gagataaaag tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata
    24001 ttagtgctta tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt
    24061 gattttttaa tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat
    24121 attgaaagaa tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt
    24181 cgtctcgctt tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa
    24241 attattacta catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt
    24301 ttttcaatag taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg
    24361 gaggtagctg ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg
    24421 gatttaaagg ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa
    24481 cgtgagagtc aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca
    24541 attgctggca tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt
    24601 caacatggaa tgtccctatc tggtgcatta tctacttata ccatactaac tattggtgac
    24661 ggattggtct ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca
    24721 agagttaatg gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat
    24781 ccttttgttc tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt
    24841 ccgttttttg tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag
    24901 gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt
    24961 gatattgata atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt
    25021 tcagaaaccg ttcctttaat tttattattt gccgaaaata agataaatgc aaatgatatg
    25081 gaaggtctta ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca
    25141 actattttat atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat
    25201 gaggtgcgtg ccgatagttt taatatatat tttgacaaag tgtgtattac agatgaaaat
    25261 ggagatatag atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt
    25321 tcttgggtag acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt
    25381 gctcaagatg aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt
    25441 tttggtatac aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta
    25501 ttaaaggaag tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg
    25561 cttggagaaa atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg
    25621 gctcccagag aaaaagatgt cataacatta gttgaacatg tccgtgcatc actttctagg
    25681 tatatttgta gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat
    25741 attgaggatg caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg
    25801 gatatagagg tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga
    25861 aatgcaaaaa aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga
    25921 cttatagata acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca
    25981 tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta
    26041 gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat
    26101 ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat taatatagct
    26161 cttgtcaatg agcaggttat gttatgggcg aattttgatg caccaagtga tgtaaaactt
    26221 cagtcatctg cttataacat attaaattta atgttaatga actttagtta tagcattaat
    26281 gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat
    26341 gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg
    26401 gaaatcttaa atgaggtctt ataatcaatg agctatacaa aattgctcac tcaattatct
    26461 tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt
    26521 gagatttgta acattcaggc tggaattgaa agtaatgaaa ttgttgcaag agctcaggtt
    26581 gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca
    26641 cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta
    26701 ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac
    26761 agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca
    26821 attgagaagc cttttttaac aggtattaag gttattgatt ctttactcac gtgtggtgaa
    26881 ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg
    26941 ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga
    27001 gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta
    27061 gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact
    27121 gctatagccg aattttttag gactgaagga cataaagtag cgctttttat tgattcatta
    27181 acaaggtatg ccagagcatt acgtgatgtg gccttagccg ctggagaatc acctgccaga
    27241 agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag
    27301 ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt
    27361 gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga
    27421 aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta
    27481 tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt
    27541 tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat
    27601 gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat
    27661 tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa
    27721 ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt
    27781 aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
    27841 acttcctgca actggagaat gacttagaac ctgtaggcgc tcagagcgtt agtcagcttt
    27901 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
    27961 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
    28021 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
    28081 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
    28141 gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
    28201 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
    28261 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
    28321 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
    28381 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
    28441 caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat
    28501 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
    28561 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
    28621 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
    28681 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
    28741 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
    28801 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
    28861 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
    28921 gctgagggta attacagatt tgatagcagt caacataata aggggaataa aaataatagt
    28981 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
    29041 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
    29101 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg
    29161 taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt
    29221 ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat
    29281 tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg
    29341 caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca
    29401 agatgccggt attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt
    29461 gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgcact ggtgatataa
    29521 tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt ggggattata
    29581 ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagaac
    29641 acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta
    29701 ttcttttaga aaaaaatatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat
    29761 tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg
    29821 ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg
    29881 taaaggagta atctcatgct gagtgacatg tccctcatag ctacactttc tttttttacg
    29941 ctattaccat ttttagttgc tgctggtacc tgttatataa agttttctat tgtttttgta
    30001 atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt
    30061 gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat
    30121 ctaaatggcc ctcaaaaatt tgatacaata tctgatattg tccgtttttc tgattctggc
    30181 ttgatggaat ataagcaata cttgaaaaag catacagatc ttgagttagc acgttttttc
    30241 caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg
    30301 ttttccctct tgcctgcata tgcgctaagt gaaatcaaag atgcgttcaa aataggtttt
    30361 tatttatatt tgccatttgt tgttgttgat cttgttattt caagcatttt acttgcactt
    30421 ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt
    30481 gcgcttgatg ggtggggtat cctgtctaag gccttgattg aacaatatat caatattcct
    30541 gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt
    30601 taatccttat cttttcctta tggccagtag gtatagctac ggttattgga ttatctattg
    30661 gtttgttaca gacagtgact caactccaag agcagacact tccttttggt ataaagctta
    30721 taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt
    30781 tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt
    30841 cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt
    30901 atttttcttt cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt
    30961 gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt
    31021 ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt
    31081 ttgcttgtct cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg
    31141 tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc
    31201 aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggta tggtctttat
    31261 tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg
    31321 tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag
    31381 tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc
    31441 tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat
    31501 tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca
    31561 taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc
    31621 gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt
    31681 aacgactgtt gttattattc tggtagggac atttactata atatcattct tttccttaag
    31741 tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa
    31801 atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg
    31861 tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc
    31921 tatcaaaatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat
    31981 aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac
    32041 ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga
    32101 tggcttatat cttatttggg gggggctgtt taaggatata atattatttt tcttggcatt
    32161 ttctattttt gttattattc ttgactttgt gattgagttc attttataca tgaaagatat
    32221 gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac actttgagac
    32281 aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat
    32341 acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa
    32401 tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc
    32461 tgtcagaaaa tatgcaaatg aagttggtat accgactgtg cgtgatgtga aattagctag
    32521 aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt
    32581 cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat
    32641 gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat
    32701 agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag
    32761 caataaggat ataataaatg ttttactcag aaagatttca tctgaatgtg acctggttag
    32821 aaaagacatt tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata
    32881 atcattcata ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc
    32941 acaggattaa ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg
    33001 ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga taagagagac
    33061 tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta
    33121 aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt
    33181 aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga
    33241 atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat
    33301 tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat
    33361 gtttactcca acaacggaga caggtaaatt atggacgaaa agaagcttaa agcccttgcg
    33421 gctgaactgg ctaaaggcct ca
//
LOCUS       DADMFL010000035        33049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.035, whole genome shotgun sequence.
ACCESSION   DADMFL010000035 DADMFL010000000
VERSION     DADMFL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.035"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            15..4592
                     /locus_tag="JNZ27_001648"
     CDS             15..4592
                     /locus_tag="JNZ27_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8664014.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4960..6615
                     /gene="lldP"
                     /locus_tag="JNZ27_001649"
     CDS             4960..6615
                     /gene="lldP"
                     /locus_tag="JNZ27_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY8664015.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6615..7391
                     /gene="lldR"
                     /locus_tag="JNZ27_001650"
     CDS             6615..7391
                     /gene="lldR"
                     /locus_tag="JNZ27_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY8664016.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7388..8578
                     /gene="lldD"
                     /locus_tag="JNZ27_001651"
     CDS             7388..8578
                     /gene="lldD"
                     /locus_tag="JNZ27_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY8664017.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8774..9247
                     /gene="trmL"
                     /locus_tag="JNZ27_001652"
     CDS             8774..9247
                     /gene="trmL"
                     /locus_tag="JNZ27_001652"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY8664018.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9300..10121)
                     /gene="cysE"
                     /locus_tag="JNZ27_001653"
     CDS             complement(9300..10121)
                     /gene="cysE"
                     /locus_tag="JNZ27_001653"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY8664019.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10201..11220)
                     /gene="gpsA"
                     /locus_tag="JNZ27_001654"
     CDS             complement(10201..11220)
                     /gene="gpsA"
                     /locus_tag="JNZ27_001654"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY8664020.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11220..11687)
                     /gene="secB"
                     /locus_tag="JNZ27_001655"
     CDS             complement(11220..11687)
                     /gene="secB"
                     /locus_tag="JNZ27_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY8664021.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11750..12001)
                     /gene="grxC"
                     /locus_tag="JNZ27_001656"
     CDS             complement(11750..12001)
                     /gene="grxC"
                     /locus_tag="JNZ27_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY8664022.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDGQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12143..12574)
                     /locus_tag="JNZ27_001657"
     CDS             complement(12143..12574)
                     /locus_tag="JNZ27_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY8664023.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12819..14363
                     /gene="gpmM"
                     /locus_tag="JNZ27_001658"
                     /gene_synonym="pgmI"
     CDS             12819..14363
                     /gene="gpmM"
                     /locus_tag="JNZ27_001658"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY8664024.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14373..15656
                     /gene="envC"
                     /locus_tag="JNZ27_001659"
     CDS             14373..15656
                     /gene="envC"
                     /locus_tag="JNZ27_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY8664025.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15660..16619
                     /locus_tag="JNZ27_001660"
     CDS             15660..16619
                     /locus_tag="JNZ27_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY8664026.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16606..17640)
                     /gene="waaH"
                     /locus_tag="JNZ27_001661"
     CDS             complement(16606..17640)
                     /gene="waaH"
                     /locus_tag="JNZ27_001661"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY8664027.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17879..18904)
                     /gene="tdh"
                     /locus_tag="JNZ27_001662"
     CDS             complement(17879..18904)
                     /gene="tdh"
                     /locus_tag="JNZ27_001662"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY8664028.1"
                     /translation="MKALSKLKAEEGIWMSDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18914..20110)
                     /gene="kbl"
                     /locus_tag="JNZ27_001663"
     CDS             complement(18914..20110)
                     /gene="kbl"
                     /locus_tag="JNZ27_001663"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY8664029.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKALEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMAEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20385..21242)
                     /gene="yibB"
                     /locus_tag="JNZ27_001664"
     CDS             complement(20385..21242)
                     /gene="yibB"
                     /locus_tag="JNZ27_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY8664030.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21547..22479
                     /gene="rfaD"
                     /locus_tag="JNZ27_001665"
     CDS             21547..22479
                     /gene="rfaD"
                     /locus_tag="JNZ27_001665"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY8664031.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22489..23535
                     /gene="rfaF"
                     /locus_tag="JNZ27_001666"
     CDS             22489..23535
                     /gene="rfaF"
                     /locus_tag="JNZ27_001666"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY8664032.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23539..24531
                     /gene="rfaC"
                     /locus_tag="JNZ27_001667"
     CDS             23539..24531
                     /gene="rfaC"
                     /locus_tag="JNZ27_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY8664033.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24528..25736
                     /gene="rfaL"
                     /locus_tag="JNZ27_001668"
     CDS             24528..25736
                     /gene="rfaL"
                     /locus_tag="JNZ27_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY8664034.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25772..26914)
                     /locus_tag="JNZ27_001669"
     CDS             complement(25772..26914)
                     /locus_tag="JNZ27_001669"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY8664035.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26923..27936)
                     /locus_tag="JNZ27_001670"
     CDS             complement(26923..27936)
                     /locus_tag="JNZ27_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY8664036.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDSVFTAIKYNV
                     MKG"
     gene            complement(27961..28668)
                     /gene="rfaY"
                     /locus_tag="JNZ27_001671"
     CDS             complement(27961..28668)
                     /gene="rfaY"
                     /locus_tag="JNZ27_001671"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY8664037.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28694..29701)
                     /gene="waaO"
                     /locus_tag="JNZ27_001672"
                     /gene_synonym="rfaI"
     CDS             complement(28694..29701)
                     /gene="waaO"
                     /locus_tag="JNZ27_001672"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY8664038.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29744..30541)
                     /gene="rfaP"
                     /locus_tag="JNZ27_001673"
     CDS             complement(29744..30541)
                     /gene="rfaP"
                     /locus_tag="JNZ27_001673"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY8664039.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30534..31658)
                     /locus_tag="JNZ27_001674"
     CDS             complement(30534..31658)
                     /locus_tag="JNZ27_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY8664040.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31655..32713)
                     /gene="rfaQ"
                     /locus_tag="JNZ27_001675"
     CDS             complement(31655..32713)
                     /gene="rfaQ"
                     /locus_tag="JNZ27_001675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY8664041.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVILAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8734 a         8087 c         7800 g         8428 t
ORIGIN      
        1 agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt
       61 atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt
      121 taatttctgc gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg
      181 ggaatgacaa cggtcagggt gttgattacg gtagtggatc agctggcgac ggctgggttg
      241 ctataggcaa aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg
      301 tgggttatga cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg
      361 cgattggtgg tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta
      421 tagcactggg tgcctcttcg tattcattgg gccaatactc aatggccctg gggcgtagca
      481 gtgtagctag cggtactgac agcctcgcat ttggcagaca atcacttgcc agcgcagcga
      541 acgctattgc gataggtgct gagaccgaag ccgctgaaaa tgcaactgct attggcaata
      601 atgcgaaggc aaaagggact aatagcatgg caatggggtt cggaagcctt gccgataaag
      661 tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca atcgccatag
      721 gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt agccagtcga
      781 gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat aagagtcttg
      841 cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc gcagattcca
      901 ttgcggattt agacaatacc gtctctgtcg gcaatagttc attaaaacgc aagatcgtta
      961 atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt tcacagcttt
     1021 atgccattag cgactcggta gcaaaaaggc ttggaggagg ggctgcagta gatgttgatg
     1081 acggtactgt tacagcacca acctacaatt taaaaaatgg tagcaaaaat aacgtagggg
     1141 ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca
     1201 gcgctgctca tggtactagt agcccaactg ccagcgtaat caccgatgtt gcggatggca
     1261 cgatttcagc ctccagtaag gatgcggtta acggttccca actgaaagct accaatgacg
     1321 atgtcgaagc caacaccacc aatatcacca acctgacgga ttccgttggt gaccttcagg
     1381 ctgatgccct gctctggaac gaaactaaaa aggcattcag tgcagctcac ggccaggata
     1441 ccaccagcaa aataaccaac gttaaagatg ccgacctgac ggctgacagc actgatgctg
     1501 ttaacggctc tcagctgaaa accaccaacg atgctgtggc gacgaatacc accaatatcg
     1561 ccaataacac ttccaatatt gccactaaca ccaccaacat ctctaacctg actgagacgg
     1621 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgta ttcacggcag
     1681 ctcatggcac cgagaccacc agcaaaataa ccaacgttaa agatggcgac ctgacgactg
     1741 gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc gtggcgacga
     1801 ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag acggtgacta
     1861 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact gcagctcatg
     1921 gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact gcaaccagtt
     1981 ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc acctacttcg
     2041 gcggcaatgc ttctgtgaat actgacggcg tgtttaccgg tccaacctac aaaatcggtg
     2101 aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca tttagcacgt
     2161 ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc aaacacggta
     2221 ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca gactccagtt
     2281 ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt gatgcgctgg
     2341 ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac accattgcta
     2401 atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact cctgacgacg
     2461 ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct cacggaaaag
     2521 ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct gccagcagcg
     2581 acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat gcgctgggtg
     2641 gtgacgcaga agtcaacgct gacggcacca tcaccgcacc gacttacacc attgcgaacg
     2701 ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg
     2761 ataagactgc caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca
     2821 tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt
     2881 ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg
     2941 ctggtaacac cgacgcaacc tatatcgaag aaaatggcgc tggtatcaac tacgttcgta
     3001 ctaacgacaa cgatttagcc tttaacgatg caagcgcctc tggtgtcggc gctacagctg
     3061 taggttataa cgctgtcgcg tctggtgcca gcagcgtagc cattggtcag aacagcagca
     3121 gcaccgttga taccggtatt gcgctgggta gcagctccgt ttccagccgt gtgattgcca
     3181 aaggttctcg tgacactagc gtaacggaaa atggcttggt gattggttat gacaccactg
     3241 acggcgaact gctaggtgca ttgtcaattg gtgatgacgg taaataccgc caaatcatca
     3301 acgtagctga tggttcagaa gcccatgacg ccgttacggt tcgccagttg cagaacgcta
     3361 ttggagcggt cgccactacg ccaaccaagt actttcacgc caactcaacg gcagaagact
     3421 cactggccgt tggtgaagac tcgctggcaa tgggtgcgaa aactgtcgtt aatggtaatg
     3481 caggtattgg tatcggtttg aacactctgg ttctggctga tgcgatcaac ggcattgcta
     3541 tcggcagtaa cgcacgcgca aaccatgcaa acagtatcgc aatgggtaac ggttctcaga
     3601 ccacccgtgg tgcacagact ggctacaccg cctacaacat ggacgcaccg cagaactctg
     3661 taggtgagtt ttctgtcggt agtgaagacg gtcaacgtca gatcacaaac gtcgcagctg
     3721 gttcagcgga taccgatgcg gttaacgtgg gtcagttgaa agtcactgat gagcgcgtag
     3781 cgcaaaatac ccagagcatt actaacctga acaatcaggt cactaatctg gatactcgcg
     3841 ttactaatat cgaaaacggt attggcgaca ttgtcaccac cggtagcacc aagtacttca
     3901 agaccaacac cgatggcgta gatgccaacg cccagggtaa agatagcgtt gctattggtt
     3961 ctggttccat tgctgccgct gacaacagcg tcgcactggg taccggttcc gttgcagagg
     4021 aagaaaatac aatctctgta ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg
     4081 ccagtgttaa tgccaccgat gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg
     4141 gagtgcgcta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg
     4201 gtggcaacgg cagtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg
     4261 cggtgaacta cgcgcagttg aagcaaagcg cgcaggaaac gaagcaatac accgatcagc
     4321 ggatggttga gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg
     4381 cttctgcaat ggcaatgacc ggtctgccgc aggcttatac accgggtgcc agcatggctt
     4441 ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga
     4501 gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact
     4561 ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg
     4621 caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa
     4681 caatctaagg gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca
     4741 caagaattgg ccctaccaat tcttcgctta tctgacctct ggttcacaat tcccaaatta
     4801 aaactcacat caatattgct aatacataac atttagttaa ccattcattg tcattatccc
     4861 tacacaacac aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag
     4921 actcattaca cgatgtgcgt ggactctagg agacctgcaa tgaatctctg gcaacaaaac
     4981 tacgatcccg ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt
     5041 ttcttctttg cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta
     5101 gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc
     5161 tcggtggttt atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca
     5221 gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt
     5281 ctttcgataa cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct
     5341 ttccttgaag gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc
     5401 ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca
     5461 gtggcatttg gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac
     5521 agctttgaga ttggccagat ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg
     5581 ttctggatca tggcgattat ggacggctgg cgcggtatca aagagacgtg gcctgcggtc
     5641 gtggttgcag gcggctcgtt tgccatcgct cagtacctta gctctaactt cattgggccg
     5701 gagctgccgg acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa
     5761 cgctggcaac cagttcgcgt attccgcttc ggtgacttgg gggcgtcaca ggttgatata
     5821 acgctggccc acaccggtta caccgcgggt caggtgttac gtgcctggac accgttcctg
     5881 ttcctgaccg ctaccgtaac actgtggagc atcccgccgt ttaaagccct gttcgcatcg
     5941 ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc cgtacctcaa taaactggtt
     6001 gcccgtatgc caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac
     6061 tggttctctg ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg
     6121 aagatgaaac cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta
     6181 cccatctact ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg
     6241 tcatcaacac tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg
     6301 ttcctcggct ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc
     6361 gccgcgctgc aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc
     6421 gccaatacca ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc
     6481 tgtgcggcgg taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac
     6541 agcctgatct tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg
     6601 tggatgattc cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg
     6661 tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc
     6721 aactggcgat gcaactcggc gtatcacgta attcactacg cgaggcgctg gcaaaactgg
     6781 tgagcgaagg cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg
     6841 acacatggtc ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg
     6901 attacagttt cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg
     6961 cggcaatgcg cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc
     7021 taagtgaaga cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg
     7081 aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat
     7141 cctcagtgaa gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga
     7201 ccgaacaaca tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta
     7261 aagcaatgat ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc
     7321 aggctcgcca cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga
     7381 aaaacgcatg attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc
     7441 gccgttcctg ttccactata tggatggtgg tgcatattct gaatacacgc tgcgccgcaa
     7501 cgtggaagat ttgtcagaag tggcactgcg ccagcgtatt ctgaaaaaca tgtccgactt
     7561 aagcctggaa acgacgctgt ttaatgagaa attgtcgatg ccggtagcac tggcaccggt
     7621 gggtttgtgt ggcatgtatg cgcgtcgtgg cgaagttcag gcagccaaag cggcggacgc
     7681 gcatggcatt ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc
     7741 agccatcaag cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg
     7801 taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat
     7861 gccgacaccg ggcgcacgct accgtgatgc gcattcaggt atgagcggcc caaatgcggc
     7921 aatgcgccgc tacttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa
     7981 cggtcgtcca catgatttag gtaatatctc agcttatctc ggcaaaccga ccggactgga
     8041 agattacatc ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga
     8101 gtggatccgc gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga
     8161 tgcgcgcgat gcagtacgtt ttggtgctga tggaattgtg gtttctaacc acggtggccg
     8221 ccagctggac ggtgtactct cttccgctcg tgcgttgccc gctattgcag atgcggtgaa
     8281 aggtgatatc gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat
     8341 gattgcgctc ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac
     8401 agcgggccag gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc
     8461 gatgacgctg actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaggg
     8521 gctgggtaaa gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc
     8581 gtttgcgccc cctcacccta accctctccc tcagggagag gggaccgttc ggcgctgtat
     8641 gtactccctc actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggag
     8701 ggccggggtg aggggaaaag gccgcacggt tcaccaattt tctgctatcc tgcccccgca
     8761 ctaagggggc agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg
     8821 caacatcatc cgtctttgcg ctaataccgg ctttcgtcta catatcatcg aaccgatggg
     8881 atttgcctgg gacgacaagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc
     8941 cgttacgcgt catcatgact atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt
     9001 tgccctcacc acgaaaggta cgcctgctca cagcgccgta agctatcagg atggcgatta
     9061 tctgatgttc ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc
     9121 tgaacaaaaa attcgcattc cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc
     9181 ggtgtcggta gtggtgtatg aagcctggcg gcagctggga tatccgggag cgttgttgag
     9241 agattagtta ctgtatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat
     9301 tagatcccat ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc
     9361 attgatggct tatcgctgtc tggtttaccg acaatacgag ccggaacgcc agcggcggtt
     9421 gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcgacca
     9481 acttcaatat tgccgaggat tttcgcgccc gcgccaatca tcacaccttc acgaattttc
     9541 gggtgacggt caccaccaga tttacccgta ccgccaagcg tcacagattg cagaatcgat
     9601 acgtcgtttt caattaccgc cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata
     9661 atgccacggc caatttttgc tgccgggtga atatcgacct ggaacgtcac agaaacctgg
     9721 ttttgcagaa agattgccaa tgcgcgacgc ccctgattcc acaaccagtg accgatgcga
     9781 taggcctgca aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact
     9841 gccgggtcgc gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg
     9901 tcagcggcgt aggcttcttc aaccacttca cggatagcaa tagcaggcat aattggcgat
     9961 gacagcttgt tcgccagcat gtagctcagt gcactgccaa ggttttcgtg cttgagtagc
    10021 gtcgcgtggt aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct
    10081 ttaatattgt tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga
    10141 gataatgacc ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg
    10201 ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat aatgtcaatg ctgcctcgcg
    10261 cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc
    10321 gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc
    10381 aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatgc caaaacggcg
    10441 gttacgcgac tggttgtcgg tacaggtaag caccagatcg cccagccccg ccatgcccat
    10501 aaaggtggca ggatcggcac ccagcgccgc accaagacgc gacatttcag ccagcccacg
    10561 ggtgatcagc gccgtacgcg cattcgcacc aaaaccgata ccgtcagaca tccccgcgcc
    10621 aatggcaatg acgtttttca ccgcgccgcc aagctgcacg ccaatgaaat ccgggttgct
    10681 gtaaacgcgg aaacttttgc cgcagtgcag cagctgctgg agatcatcgg caaaggtctg
    10741 atcggtcgag gccagcgaaa tagctgtcgg taaacctgcc gccagttctt tcgcaaacgt
    10801 tgggccagag ataaccgcca gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg
    10861 taacagacgt ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg
    10921 acgcatcagc ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac
    10981 gagaatatta cgactggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc
    11041 gggaaaaggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat
    11101 atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtaatggc
    11161 aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat
    11221 caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac
    11281 agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg
    11341 ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa
    11401 tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca
    11461 cacaggaacg cggtttcttc gcccaaggag gccgttacgg taacacgcag taccacttcg
    11521 tatacgtcat ctgccagttg ggaagatgcc gtatccagat caagtttaac ttctggttgc
    11581 caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagagatatc cttggtataa
    11641 atacgttgga tctggaaagt catttcagtg ttgttttgtt ctgacatgtg tagaaaaccc
    11701 ttaagtgttg tccttaaata ctgcgcaatg taatgccaac gttcacacgt tatttcagca
    11761 ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct
    11821 gtccgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac
    11881 gcttggcggc attgccatcg atcggcaact cctggaaact cacgcccttg ctgctcagca
    11941 gtgcttttgc acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca
    12001 taatttagct cctgtttttt taccctgaaa tttcatgttg cagggcggct gcaaaatgga
    12061 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    12121 atcatgaaag acgacaggta aattatttgc cgcgcaccag aggcaagttt tcgccagccc
    12181 agccagcaac gccctctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcgt
    12241 ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccgat ttgtctttgt
    12301 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac
    12361 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    12421 ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc
    12481 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    12541 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc caacgtagtg
    12601 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    12661 actggaatgc aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc
    12721 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    12781 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    12841 tggtactggt gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt
    12901 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    12961 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    13021 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    13081 tcaaagatcg cgctttcttt gctaatccag tgctggctgg cgcggtacat aaagcgaaaa
    13141 acgcaggtaa agcagtacac attatgggtc tgctctccgc aggcggcgta cacagccacg
    13201 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    13261 tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa
    13321 aatttgaaga gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    13381 actacgcgat ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga
    13441 ctctggcgca gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg
    13501 ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    13561 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    13621 gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg
    13681 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    13741 acccacccgc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    13801 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    13861 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    13921 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggccatca
    13981 agagcggcaa atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca
    14041 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    14101 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    14161 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    14221 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg
    14281 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    14341 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    14401 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    14461 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    14521 ctattcaggc cgatatcgcc gcaaaagagc acgcggtacg ccaaaagcaa caacaacgcg
    14581 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    14641 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    14701 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg
    14761 atgccgcatt ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa
    14821 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    14881 ccattgccca gttgaagcaa acgcgtgaag aagtcaccat gcagcgtgct gaactggaag
    14941 agaaacaaag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga
    15001 ctcaggttct gaacgagcgt aaaaagacgc tggcggggct ggaatcttcc atccagcaag
    15061 gtcagcagca gctaagtgaa ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc
    15121 gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc
    15181 gcgagcgcca gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa
    15241 aatcgctgat gtcccgaact ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg
    15301 ttcgcgggcc gacgctgcat cgctatggcg agcagctaca gggtgaatta cgctggaaag
    15361 ggatggttat cggtgcctct gaaggtactg aagttaaagc aattgccgac ggtcgagtga
    15421 ttctggctga ctggctgcaa ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg
    15481 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    15541 cgggccagcc aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    15601 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    15661 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    15721 tacttgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    15781 accaggtgct ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    15841 ccagagagat ggcgaccaaa gcgcataaca gcggtcacga agtgttgatt catctcccga
    15901 tggcaccgtt gagtaaacag ccgctggaga aaaatacgct acgcccggag atgagcagcg
    15961 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    16021 acaaccacat gggcagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    16081 cgctggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaggcga
    16141 tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt
    16201 cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca
    16261 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    16321 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    16381 cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt
    16441 tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    16501 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    16561 agtggcaagg ctggggcaaa cagcacgaag cggcgaagtt taacgctagc gcaaattaaa
    16621 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    16681 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    16741 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    16801 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    16861 ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    16921 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    16981 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcgtt
    17041 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    17101 gttattttta acaataacat cacggcgata aacccccatc cagacaacgt gagtccaacg
    17161 gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacaccgg ttgagcgaag
    17221 gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaagacc agtcagcgtt
    17281 gcactgcgcc acgtcgaggt cgtcctctaa cgccatggtc atcagcgttt cgtacatggt
    17341 gggatagacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg ccacctcgat
    17401 cccacgatta cgcgctaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    17461 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    17521 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    17581 atcatcgccc gcattatata acggaataat aacactaagt ttatttgtgc tgttcatcat
    17641 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    17701 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    17761 gagaacagct atataaaaga tcagcgcaat tacgaactct ttattacact gacaggattt
    17821 tacataatta acagattatc ctcagataaa agggctggaa taccagccct tgttcgtgtt
    17881 aatcccagct cagaataact ttcccggact ggcccgaacg catagcgtca aagcccttct
    17941 ggaaatcatc gatagagaaa cgatgggtaa tgatcggcga gagatcgagg ccagactgaa
    18001 tcagcgccgc catcttgtac caagtttcaa acatctcacg accgtaaatg cctttaatga
    18061 acaagccttt aaagatcact ttggtccagt cgatagacat atcagacggt ggaataccca
    18121 gcatcgcaat acgaccgccg tggttcatgg tatcgagcat ggtacgaaac gctggcggcg
    18181 cgccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttca gccatcacat
    18241 cattaaggtt ttctttcgca acgttaaccg cacgggtgat acccattttg cgcgccagct
    18301 caaggcggta ttcgttaaca tcggtgatca ccacattgcg tgcaccaacg tgtttcgcca
    18361 ccgccgcagc cataatacct atcggacctg caccagaaac cagcacatct tcgcccacca
    18421 gatcgaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccaggt
    18481 cgtcggagat attgtcgggg attttgaaag cgttgaacgc cggaatcacc agatattcgg
    18541 cgaagcagcc cggacggtta acaccgacac caatggtgtt gcggcacaga tgggtacgac
    18601 cgccacgaca gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc
    18661 cgattttgaa gcctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    18721 ccacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    18781 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    18841 ccggtacagg gacgtcgctc atccagatgc cctcttccgc tttcagtttg gataacgctt
    18901 tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    18961 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    19021 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatgccctct ttttgcaact
    19081 ctcgggcaaa tttctgcgcc actaccgcat caccaagcat gaccggaata atggcgtgat
    19141 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga cgcgcgttcg
    19201 cccacagacg gtcacgcaac tcgctacccg cttcagccat ctccagcact ttgatggaag
    19261 cggcgacaat cgctggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca
    19321 gccactcaac cacttctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt
    19381 taccgagcgt gccggtgatg atgtcgacac gccccatcac atcgcagtat tcatgggaac
    19441 cacgaccatt ttcaccgaca aaaccgaccg cgtgggagtc gtctaccatc accagggcat
    19501 cgtatttatc tgccagatcg caaacgccct tcagattggc aatcacgccg tccatcgaga
    19561 acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    19621 gagcttcaag ctcctgcata tcgttgttgg catagcgata gcgtttagct ttacacagac
    19681 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    19741 ccagcagcgt ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    19801 cttccatccc caggaatgcc gccagttttt gttcaagcgc tttatggctg tcctgagtgc
    19861 cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgctg
    19921 ccgcaatcag atccggatga ttcgccagcc cgagatagtt gttggcacaa aagttaatga
    19981 cgtggcttcc atcagccacg gtgatatctg cttgctgcgc agacgtaata atgcgctctt
    20041 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    20101 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    20161 ggtcgcgacg agatgatgcg ttaacacttc tgcaaaattc aggataaata acgtgtatct
    20221 tgatgtctct caacacctac agatagtaga aaaactgtac agttttggct gagcataatg
    20281 atgagtgatt ttactgcatt gatataaaaa gaaaaaatga tgccaaagca tcatttttcc
    20341 tatctcagtt ggcatcaaaa cgctacccac taatggaggg aaacctattt tcttgaaagg
    20401 aaaattctca gcgcctgcat ttttgcccct aaagtgttac tcctgaagca gcgaaacaaa
    20461 tcgaaccatt ttcctctccc cagatagttt aggttgaaga gatcggaacg tttgtaatag
    20521 cacatgacaa aaatgccctg gtcatcgtcc acaatgttat tgtttagtgt tattttttga
    20581 ctttccagaa ctaatttata gaattctttc catttatgct gtgaaccaac aattgcgccc
    20641 ccaataatat atacatgatt gccaatcatg aaatcaaaag cctgttgttg agatgtaaca
    20701 gtaagccctt tttttatagt gaaaagatgc atcttatttt catcaaatgg gaaatcccaa
    20761 attttcaatc ctcgagttac atttggctta cggcaatagc caaaatctat ccacgcaaca
    20821 agaggcgttt tcactaaacc catgttgata gctttattta caaaataggc cttaaggtta
    20881 cataccaaaa catactctgg tgaccagtac tctgggtttt ttaattgtcg aggctcaagt
    20941 ctatttgtaa acgactcatc tttttgaatt ttttcgatac gacttctgat atatctaaat
    21001 ttctttttta tatcgataac gataaccgtt gttggttttc cgttgcgaat cgcttcaatc
    21061 cttggtttca aatcgggaga agtgaaaata atcatctcat tttcaagtgc ggccagacgc
    21121 tcaaagtaac taaaataaac atcaactgaa cgagctagtt tttcacggaa ccctttatta
    21181 gcagtccaat cccctctacc aatgtcaaaa tatgcagtga taattgtggt ggatgatttc
    21241 attactaata atgaccatta atataaaaaa agaattatat cataaatatt aacagaatgt
    21301 atactgtata ttatccattc aaatgattcc gttatttatc atacatcgga atattgatac
    21361 taaagcacta ttcacatgca aaaccaacat ccgccatgaa ggactagcta aaacccaaac
    21421 tagtttgttg caattagcat ccttgcacct ctatgtaaag ggctgaaggg attcggatgt
    21481 gatggtatga ttacagacat tcgtgtctga gattgtctct gactccataa ttcgaaggtt
    21541 acagttatga tcatcgttac cggcggcgcg ggctttatcg gcagcaacat cgttaaagcc
    21601 ctgaatgata aaggcatcac cgatattctg gtggtggaca acctgaaaga cggcaccaag
    21661 tttgtgaacc tggtggatct ggatatcgcg gactatatgg ataaggaaga cttcctgatc
    21721 cagattatgg ctggcgaaga gttcggtgat gtcgaagcga ttttccacga aggcgcatgc
    21781 tcttccacca ccgagtggga tggcaagtat atgatggata acaactatca atactccaaa
    21841 gagctgctgc actactgtct ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc
    21901 acctacggcg gacgcacctc cgactttatt gaatcccgcg agtacgaaaa accgttgaat
    21961 gtctacggtt actcaaaatt cctgtttgat gaatatgttc gtcaaatcct gccagaagcg
    22021 aactcgcaga ttgttggctt ccgctatttc aacgtttatg gaccgcgtga aggccataaa
    22081 ggcagcatgg cgagcgtcgc tttccatctc aacacccagc ttaataacgg tgaatcaccg
    22141 aagctgttcg aaggtagcga gaacttcaaa cgtgacttcg tctatgtggg cgacgtggca
    22201 gatgtaaacc tgtggttcct ggaaaatggc gtttccggta tcttcaacct cggcaccggt
    22261 cgtgcagaat ccttccaggc agtagcagat gctacgctgg cttatcacaa gaaaggccag
    22321 atcgaataca ttccgttccc ggataagctg aaaggccgct accaggcgtt cactcaggca
    22381 gatctgacaa atctgcgcgc ggcgggttac gacaaaccgt tcaaaaccgt tgctgaaggt
    22441 gtaacggaat acatggcctg gctgaatcgc gacgcataag agctctgcat gaaaatactg
    22501 gtgatcggcc cgtcttgggt tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc
    22561 caggcgcgct atccccaggc gataatcgat gtgatggcac cggcatggtg ccgtccatta
    22621 ttatcgcgga tgccggaagt taacgaagct atccctatgc ctctcggtca cggagcgctg
    22681 gaaatcggcg aacgccgcaa actgggtcat agcctgcgtg aaaagcgcta cgaccgcgcc
    22741 tacgtcttac caaactcctt caaatctgca ttagtgcctt tcttcgcggg tattcctcat
    22801 cgcaccggct ggcgcggcga gatgcgctac ggtttactca acgatgtacg cgtgctcgat
    22861 aaagaagcct ggccgctaat ggtggaacgc tatgtcgcgc tggcctatga caaaggcatt
    22921 atgcgtaccg cacaagatct gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa
    22981 ggtgaaaaat catatacctg taatcaattt tcgctttcat cagaacgtcc gatgattggc
    23041 ttttgcccgg gtgcggagtt tggtccggca aaacgctggc cacactacca ctatgcggaa
    23101 ctggcaaagc agctgattga tgaaggttat caggtggttc tgtttggctc tgcgaaagat
    23161 catgaagcgg gcaatgagat tcttgccgct ttgaataccg agcagcaggc atggtgtcgg
    23221 aacctggcgg gggaaacaca gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc
    23281 attgtcacta acgattctgg cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt
    23341 gccctgtatg gtccaagtag cccggacttc acaccgccgc tatcccataa agcgcgcgtg
    23401 atccgtctga ttaccggcta tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac
    23461 cagagcttga tcgacattac tccccagcgc gtactggaag aactcaacgc gctattgtta
    23521 caagaggaag cctgacggat gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt
    23581 ctccatacgt tgcccgcact cactgatgcc cagcaggcaa tcccagggat taagtttgac
    23641 tgggtggtgg aagaagggtt cgcacagatt ccttcctggc acgctgccgt tgagcgagtt
    23701 attcctgtgg caatacgtcg ctggcgtaaa gcctggttct cggcccccat aaaagctgaa
    23761 cgcaaagcgt ttcgtgaagc gctacaagca gagaactatg acgcagttat cgacgctcag
    23821 gggctggtaa aaagcgcggc actggtgaca cgtctggcgc atggcgtaaa gcatggattg
    23881 gactggcaaa ccgctcgcga acctttagcc agcctgtttt acaattgtaa gcatcatatt
    23941 gcaaaacagc agcacgccgt agaacgcacc cgcgaactgt ttgccaaaag tttgggctat
    24001 agcaaaccgc aaacccaggg cgattatgct atcgcacagc attttctgac gaacctgcct
    24061 acagatgctg gcgaatatgc cgtatttctt catgcgacga cccgtgatga taaacactgg
    24121 ccggaagaac actggcgaga attgattggt ttactggctg attcaggaat acggattaaa
    24181 cttccgtggg gcgcgccgca tgaggaagaa cgggcgaaac gactggcgga aggatttgct
    24241 tatgttgaag tattgccgaa gatgagtctg gaaggcgttg cccgcgtgct ggccggggct
    24301 aaatttgtag tgtcggtgga tacggggtta agccatttaa cggcggcact ggatagaccc
    24361 aatatcacgg tttatggacc aaccgatccg ggattaattg gtgggtatgg gaagaatcaa
    24421 gtagagtgtc gttctacaag tatgtctctt gcagatttgc cagctcaaac cgtttttcaa
    24481 aatttaaatc ttgaaataat aaccaataag ttgacatcgg agataagatg acctcaacat
    24541 tatttttctc tctcgagaaa aaaaactgga tagcgtactg gaacagagct ctcgtattct
    24601 tattcattac cacctatttt ttgggtggga ttacaaggta taaacatctt attgttattc
    24661 ttatgacaat aacgacaatc gtctatctct gcaaacggcc aaaacactat ctctcactat
    24721 ttaaaacatt tctttttggt agtgttgcca tattaactat tgctgcattg ctgtcacttc
    24781 ttcaatcccc tgatgcaggt gctagcatga aggaaatttt caaagctatt attgagaata
    24841 ctttactatg cacaatagca ataccggtca tattgagaga cgagaaaaga gaagatgtcg
    24901 aaaaaatcgt tttcttctca tttattagtg cgttgggctt acgctgtttt tctgaattga
    24961 ttacctatta taaggactat caacaaggga taatgccatt cgcagattat agacaccgta
    25021 gcatttctga ctcgatggtc tttttattcc ctgcattgtt aaatctctgg cttatcaaat
    25081 cagcaaaata ccgcatttct tttgtggttc taagcgttat ttttattttt ctgatattag
    25141 gaactttatc cagaggggcc tggctttccg tgttagtcat tggattaata tggattctga
    25201 tgtttaaaca atggaagtta ctattagtag gagtaatggt ttccatcatt gcattgtcgg
    25261 ttattttcac acataaggag atgaccgcaa agctaacgta taaacttcaa caaactaata
    25321 gttcttatcg ctatgcaaat ggtactcaag gcagcgcact cgatctaata ttagaaaatc
    25381 ctgttattgg ttatggttac ggtaacgttg catataaaga tgtctataat aaacgtgtca
    25441 ttgattatcc agaatggacc tttagacaat caatagggcc acataatttt gcgctattca
    25501 tctggtttgg cactggttta ttagggctgg taagtcttat gatgctatac tgtgcaatat
    25561 tgaaagagtg tataaaaaat ggcgtcaaga ataaatatcg ctcaccatat aatgcatatt
    25621 atataatctt actatctttt ataggttatt ttgttatccg tggaaacgta gaacaaattg
    25681 aaccaaattt attaggcgtt tacgccggct tattattagc gatgaaaaac aagtaaaaaa
    25741 taaaaaaggc tgcataatgc ggccttttta tttattcaaa ccaattatga ataacctctt
    25801 cgaatctttg agttacgcca tcccaactgt agtgatcaaa cacaaagtcc tgaccttgct
    25861 ttgcaactgc tgttaattca ggattcgcta acgtttttaa aatatcgctg cttatggagt
    25921 ctgcagtcat aggttctttt aaatggaatc ccgtagtatt ttctttgaca aattccgtca
    25981 tccccccacg cgtactcact aaaacaggct taccagcacc catagcttca attgccacca
    26041 tgcaaaatgg ttcctggaac tgggaaggaa tgacgactaa atctgccagt gggtaataac
    26101 aatgcatctt ttcaggcggt atgcttccta acataatgca acgatccttc aaacgttttg
    26161 caatttccct gacattgcgt tgatatgcac ctttgtcact tttactcatt tctgtataat
    26221 caccaatcac aactaatttt aaatttttgt gagcagcggc caatttttcg aatgattgca
    26281 tcaacagcaa tatgcctttg tcagggacga tacgcccagc ataaaaaata attttctctt
    26341 caggggagat attaagttcg cttctagtga tcggctgaaa attagattga taggtttcta
    26401 aatcaattcc atttggtaca atctcaatat ctgcatttgc taaataagat tggtaatact
    26461 ttttcaagta taaactaggg actatcattt taacattttg ctcaagcaat tttggttcaa
    26521 atgcattatg catatgtata accactcttg cctgaggagc tcgcttacga atttgagtgt
    26581 atagcttcat gctattatga actacaataa cactttcttt cgttatagga aaatcatgtg
    26641 caatattcaa tattctctgt gaatatggaa gaggatcaag tcttgtccat ttttgaaata
    26701 accgcttata tatgcgactg aatccaaccc tatgaatact acaattatca ttaacaaagg
    26761 tgtaatttga gtatcctgga tttttgatac aaacaatacg atttggaaaa ttggtacgct
    26821 gagcaacctg atacatccac gtttcaacag cagccgctcc gcgaggagga attgaaaaaa
    26881 taggagtaac cgtaaatatt attttatcaa ccatctcgta tattaacctt tcataacatt
    26941 atatttaatt gctgtaaaaa cactatcgag aaattttttc tggtaaagca agtgtttata
    27001 tttttctttg tgctcatgtt ttttaacagc ttttttataa ggtatatttc tccatggtga
    27061 tatattataa atattacgaa aataatctgc agaggcgtag ttcgcccaat catgccatgg
    27121 cttagttata ccagtataat gtatgaagac agtgtcatca ttaataaacc gggtgtaata
    27181 ttcactattt ttttcttcaa attctgactt tatagtataa atacaattat attttctcgg
    27241 taatatttta gcatgatgta gaaacattaa gtttaaaata tcttgatccg ggtactttaa
    27301 tttttttata attgactcat ctgataatag atcaaagaat ttttccgtta gtctttgttt
    27361 taaccattcc cgtaaattaa tatacattac accagagtta aaatagctac cgttaaaatc
    27421 ctcattacac aaacgctcag cgcttttact ttgcatagca tctacatcaa ttaccactgc
    27481 cccatattca tctttaaact ctaatgctat taactcgttc aatgaaccct tacagacgat
    27541 atcagcatca agatataata atgaatccaa tcgcgcagaa aaataatcaa atgaaagcaa
    27601 acgataataa atagatactg gccagatatt tgattgaggt agcgccttca atggctcaga
    27661 atcaatatga tataatttaa ttactgtttg atacttcgtt gctaattgtg ataataattc
    27721 aatatactct ttgtctaagt aatcagcgat tatgtgaaag gtaaatttgt tttgtttatt
    27781 cttttcaaga attgaagcaa ttgaaatcgc agccccaacc tggtagttct catcaatacc
    27841 ccatgagaca ttaaacgttt cccgttcatc aagtttaacg gggcgcttgt ccagttcaat
    27901 tatatcttta aattgagtaa gatgtttaaa atccaattta attacccttc atcataatca
    27961 ttatttgctc ttcaatccta gtttgacttt tacatccctg attacttttt taattttctt
    28021 tttgaatata agatatgtat agccaaagtc tttcaattca ttttttatat tatagtgccg
    28081 ttcaagatca atgcgatctt ttgcttttaa aacagcggtt gtttttttac ctgaaaggtc
    28141 aattagtctt aaaccttttt cggatactat aaaatttcct ttatgaggat cgccagacac
    28201 cattccatgc tgatgcaact cttttatcga ttctgataat tgatccttca gatcttcaga
    28261 aatttccaaa tactcattta acccgacacc ttcaatatac tcaatgagca taatataata
    28321 atgagcgaaa tttaatgttt tacgttcagc tagcaaaaaa tagtcattaa ttgattggat
    28381 gccttcacca cgtacccgat cggtttgata aattaaattt tcataataat ctttttttat
    28441 gcaagatttt aagaaacgtt cggtcatttt atgctttggc gcataaactt taagtacaag
    28501 aggtccacga gcagtgtcaa taagaatgac tttagtatca tcaatagaac gaaaaacttt
    28561 taatgtttta accctgcaag ttaaaaagtc atttagaact tgctcataaa agggatcatt
    28621 atcttttatg aaaactttca acccatttat cgttttatta tatatcataa tgaattattt
    28681 taaccttaaa tctttagaag catttttctt tataatactt taaataatta aaaatgcctg
    28741 ccatataatg cttttgttta aatttatgtt ttgcacaata ccgatactga ttgctattta
    28801 caggtttaag taaatcttct ttactccacg gagaagctgc tttggcgatc aaaaaactac
    28861 gtgaaaccgg ataatcagcc cactcatgcc agggttttgt aggtcccaca tagtgtataa
    28921 acacggtgtc atcattgact ggattatcaa ctttgtcttt taattcatag ttaatactat
    28981 atcgggtatt atatttctcc gaaataaact tcactttacc attcaataat acattcagta
    29041 catcttgatc aaggtgggtg attttactta cccaatctgg gtcacgcaac atttcaatag
    29101 cttttgacga aatgttattt agattccact catcaatatt tatcagtaaa aaaccagcat
    29161 tgaaatatcc agaagctaac tgtggtgtag ttaataccga ggctcgattt tgccaccatt
    29221 caatatctct ttcagctaca actgcggcaa tttcattagt agaaaattga taatctaaga
    29281 gttctttaat actacccttg caagcaatat ctgcatcaag atatagtatt ttttcatgtt
    29341 tatgatagaa ataatctgca attataaatc gaaaatatgt tgcgtaagtc cagtttttcg
    29401 tgcttggtaa tgacttcagc ttatcacaat tgataacata aatattaatt cttgaattat
    29461 attgttttgc taaatcagaa aaatataatt tgtctttatc actgatataa tctgtgaaaa
    29521 catgaaattc acaagagatt tctctattgt ttaatagaat agatgcgatg gctacaccac
    29581 atccaaaaag aaaatttctg tcaataccaa atgctatatc aagagtattt ttagatctta
    29641 tattttgata atgaaaatta tactcaaaaa ggatgatgtc actatcattg agttgagaca
    29701 tatttattct gtatccaaaa ttttggcaaa cataagttac gggttacaac gattttcgaa
    29761 tcgttctttc cctgattttt gtggcttttg cttctgcttg cgacagcagt ccttgttcct
    29821 gcttgagaat gtctttaagc ggggcggcaa aatacacctt cataaagcgc cagatatccc
    29881 gctgagtcag gccaatattc attgaagaaa aataaagccc tataagatct ttatcgcgcc
    29941 aacgacctgg aacgcgcgtg cgaagctgcg cccggtgtag gtcaattacc gaaattttta
    30001 actcctcttc cttaccggaa aaaggcaagt gcagcaggaa atgacagata taacagtcac
    30061 gatggttaat gcccgcagca tgcatatcgc gcaccatcgt cgcgacacgc ttaataagca
    30121 tacgctttac gcgaacatct ggtgggttgg tcgcccagtc agcacagtaa tcttccagac
    30181 tgatggttgg tgtcagatct tcggtaataa taaacgaggt tctggtcagc ggattcatgc
    30241 ctttttcgcc gaatgccacc ccatacatag tatcaacgcc gacatcccgc agtcgatgaa
    30301 tcgcattcca ttcgcggtct gcgcctaata ctggcatccg caatgagagt aaatttttga
    30361 ttatctcttt cagggtcgtg ccgcgatgcc atttgagaaa atagcttttt cccgccattt
    30421 caaagcgcag agtacggcga gtttccagtt cacgaaatac ctcaccctgc aaggttttaa
    30481 cttcctcaaa aggatctttg ccgcgccata acgtggcaaa cggctcttta agttcaacca
    30541 tccagaccac ccgttatgat atccgccgct ttctctggca gactgtataa atcttgtgta
    30601 tcagcataat gtcgcgcatt ttccgcccaa gcctggcgca atgaagattg cgttaacgct
    30661 ttgcgtaaaa tctcattcaa tgtttcctgg cggaatggct cagcaatagc ctcgccgcaa
    30721 ttagcgtcga caatataatg cgcatagcca caaacggcag ttgttagtac cggtaatcct
    30781 gcagtaatcg cttccagcag cacaattccc gccgcttcct ggtaggcagg atgcagtaat
    30841 aaatccgccg ccgccattaa ttccgagaca tcgttgcgcc ccgagaagaa gtgaacatta
    30901 ctgcgcacgc cgcgtttttc tgccagtgcc tcaaattttc gcggtttatc ctgcccaaca
    30961 acatatagca atgtgttgtg gcgcagcgaa tccggtaacg aagcaagtgc ttcaatggaa
    31021 cgatcgacac ctttacgcgt gaagtctgaa ccgacctgca acaataaata ttgttgttcg
    31081 gttattccat tcttcttacg gaagatttca cggctattgg ctggctgctg gctatattta
    31141 cgatcaggat aaatccccgg tggcagaata tgaaaacgct ccgcttcagt ctgataatgt
    31201 ttctggaaat cggcgatttg cttatctgtc agcatcagca gctgtgtcgg cttgccctgt
    31261 tcgaaggttg cccgctcaaa ggcggcataa tggcgataac gtgacgtcag gcgatagaaa
    31321 aagccttttt cctgcgctac tttctcggca taacaaacat cagcggcata ataaacgtcc
    31381 agccccggca ttttgttgaa tccaacgact ttatcgacgg gatgttcgcg taaatgtttt
    31441 tgcacccagg caaaatactc cgcattgcgc ccgtgattgg tatgcgattt aaccggcact
    31501 ttgatcagtt caaatacatc agggcattcg ccttcccacg actgggtata aacccgaaca
    31561 tgatgacctc gggcggcgac tgtctgagca atacgcataa aatcgcgctg caaaccgcca
    31621 aagggaaaat atttatataa acaaaaagca acgatcataa cgacgtacct gtcgtggagg
    31681 agggcagtaa tttatcgaca gcagcaatga catctgccgc cagaataacc gaaagataca
    31741 tctcatttcg gtcacgctga tcgcgcgttg gcatttcccg gtaatctccc gcccagaatt
    31801 gaatcatgtt atttgaccag ggacgccaga aaatatgatc tgttgcgcca aacagcgata
    31861 tcagcggcgt attaactgca gcggcaatat gcgccggtgc ggaatcaacg ccaataaaca
    31921 gctgcgcatg atcgattaac gcaccaagtt ccgggaaggt cacctttcca gccagcgccg
    31981 ttactggtgg cgtctggcat ccctgcgcaa tttcattgac gcaggccagg tcgtctttat
    32041 ctgggccgga cgtcagaacg acttcataac cacgagcatg taaggcatca atcacagcgg
    32101 aaaacttggc gttgtcccag catttgaaga tttgccgcgc cgtaggttgg ataaccacat
    32161 aattttgtcc aacaccagcg tgatcaagtt cgcgacgcat acgtttccag cttgcaggcg
    32221 ggtaactcat ggttgtctgc ttcaccaacg aatcaagtcc caatggtgtc agcacggata
    32281 agttactttc caccacattt ccaccctgca acggcaccaa atgggtgaaa cttttacgcc
    32341 aaaaagcaga ctgccgatga tgataatcct gggaaatttt cacacgagca tttaataagc
    32401 gaaccagtat agcaaccatc cattgatcgg taagattgac gataaggtca tacttattgg
    32461 cacgtaatac cttgatgaga tgaaaaaagt tggcaatttt ttctgaggct tttgcttttt
    32521 tattttttat gccgtagagc gcgttaatct ctggattttc agacaggatc gggatggtgt
    32581 cctgataaag cagcacatcg atttttgcgt cagggtaatt ttttttcaga gaactaatga
    32641 cgggagtagt taataacata tccccatgaa aacgcatctt aatgagcaaa attcttcgaa
    32701 atggcttatc cacaagtaac tctttttgtt gagattatcc gattaaggtg aagcagaaaa
    32761 agcacgctac cgccccaggc tcaacagcta cctgaatact gataccgcac tgatattgac
    32821 gcgctagtgt atcacttctt tcgcggcaat ccgacccgaa tgatttttaa gcacattgtt
    32881 gattttcaat aaagaaatca atcaaataaa atcattagca accatattct ttcactgttg
    32941 ctctgcgatt ccttcctgca cgtacagcag aaatcaatga atcgcataaa aatacaaaaa
    33001 aagtatagat gttcagcaag aattggcggg taaattgcta tttaagtca
//
LOCUS       DADMFL010000036        33007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.036, whole genome shotgun sequence.
ACCESSION   DADMFL010000036 DADMFL010000000
VERSION     DADMFL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.036"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..1159)
                     /locus_tag="JNZ27_001676"
     CDS             complement(<1..1159)
                     /locus_tag="JNZ27_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664042.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAII"
     gene            complement(1337..2248)
                     /gene="gsiD"
                     /locus_tag="JNZ27_001677"
     CDS             complement(1337..2248)
                     /gene="gsiD"
                     /locus_tag="JNZ27_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY8664043.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(2251..3171)
                     /gene="gsiC"
                     /locus_tag="JNZ27_001678"
     CDS             complement(2251..3171)
                     /gene="gsiC"
                     /locus_tag="JNZ27_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY8664044.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3189..4727)
                     /gene="gsiB"
                     /locus_tag="JNZ27_001679"
     CDS             complement(3189..4727)
                     /gene="gsiB"
                     /locus_tag="JNZ27_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY8664045.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKTNLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4747..6618)
                     /gene="gsiA"
                     /locus_tag="JNZ27_001680"
     CDS             complement(4747..6618)
                     /gene="gsiA"
                     /locus_tag="JNZ27_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY8664046.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6605..7570)
                     /gene="iaaA"
                     /locus_tag="JNZ27_001681"
     CDS             complement(6605..7570)
                     /gene="iaaA"
                     /locus_tag="JNZ27_001681"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8664047.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7774..9009
                     /gene="moeA"
                     /locus_tag="JNZ27_001682"
     CDS             7774..9009
                     /gene="moeA"
                     /locus_tag="JNZ27_001682"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY8664048.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            9009..9758
                     /gene="moeB"
                     /locus_tag="JNZ27_001683"
     CDS             9009..9758
                     /gene="moeB"
                     /locus_tag="JNZ27_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY8664049.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9937..10599)
                     /gene="fsa"
                     /locus_tag="JNZ27_001684"
     CDS             complement(9937..10599)
                     /gene="fsa"
                     /locus_tag="JNZ27_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8664050.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10730..11629
                     /locus_tag="JNZ27_001685"
     CDS             10730..11629
                     /locus_tag="JNZ27_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY8664051.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11635..14067
                     /locus_tag="JNZ27_001686"
     CDS             11635..14067
                     /locus_tag="JNZ27_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY8664052.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            14213..15028
                     /gene="ybiV"
                     /locus_tag="JNZ27_001687"
     CDS             14213..15028
                     /gene="ybiV"
                     /locus_tag="JNZ27_001687"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY8664053.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKYYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            15180..16445
                     /locus_tag="JNZ27_001688"
     CDS             15180..16445
                     /locus_tag="JNZ27_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY8664054.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16686..18278)
                     /locus_tag="JNZ27_001689"
     CDS             complement(16686..18278)
                     /locus_tag="JNZ27_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY8664055.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18497..19417
                     /gene="ldtB"
                     /locus_tag="JNZ27_001690"
     CDS             18497..19417
                     /gene="ldtB"
                     /locus_tag="JNZ27_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8664056.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19475..20593)
                     /locus_tag="JNZ27_001691"
     CDS             complement(19475..20593)
                     /locus_tag="JNZ27_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY8664057.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20590..21057)
                     /locus_tag="JNZ27_001692"
     CDS             complement(20590..21057)
                     /locus_tag="JNZ27_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY8664058.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21243..21371
                     /gene="mntS"
                     /locus_tag="JNZ27_001693"
     CDS             21243..21371
                     /gene="mntS"
                     /locus_tag="JNZ27_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY8664059.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21643..23226
                     /locus_tag="JNZ27_001694"
     CDS             21643..23226
                     /locus_tag="JNZ27_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY8664060.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(23274..23789)
                     /gene="ompX"
                     /locus_tag="JNZ27_001695"
     CDS             complement(23274..23789)
                     /gene="ompX"
                     /locus_tag="JNZ27_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY8664061.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23842..23907)
                     /locus_tag="JNZ27_001696"
     CDS             complement(23842..23907)
                     /locus_tag="JNZ27_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664062.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            24142..25029
                     /gene="rhtA"
                     /locus_tag="JNZ27_001697"
     CDS             24142..25029
                     /gene="rhtA"
                     /locus_tag="JNZ27_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY8664063.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKEGKIKELDIN"
     gene            25328..25831
                     /gene="dps"
                     /locus_tag="JNZ27_001698"
                     /gene_synonym="pexB"
     CDS             25328..25831
                     /gene="dps"
                     /locus_tag="JNZ27_001698"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY8664064.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            26235..26981
                     /gene="glnH"
                     /locus_tag="JNZ27_001699"
     CDS             26235..26981
                     /gene="glnH"
                     /locus_tag="JNZ27_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY8664065.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            27120..27779
                     /gene="glnP"
                     /locus_tag="JNZ27_001700"
     CDS             27120..27779
                     /gene="glnP"
                     /locus_tag="JNZ27_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY8664066.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27776..28498
                     /gene="glnQ"
                     /locus_tag="JNZ27_001701"
     CDS             27776..28498
                     /gene="glnQ"
                     /locus_tag="JNZ27_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY8664067.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28920..30983
                     /gene="ybiO"
                     /locus_tag="JNZ27_001702"
     CDS             28920..30983
                     /gene="ybiO"
                     /locus_tag="JNZ27_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY8664068.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(30980..31906)
                     /gene="rlmF"
                     /locus_tag="JNZ27_001703"
     CDS             complement(30980..31906)
                     /gene="rlmF"
                     /locus_tag="JNZ27_001703"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY8664069.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            32182..32442
                     /locus_tag="JNZ27_001704"
     CDS             32182..32442
                     /locus_tag="JNZ27_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8664070.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32707..>33007
                     /locus_tag="JNZ27_001705"
     CDS             32707..>33007
                     /locus_tag="JNZ27_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY8664071.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         8109 a         8805 c         8146 g         7947 t
ORIGIN      
        1 caataatcgc catattttta ataaaaccgc tttcctgatc gtaagagatc agtgagagca
       61 atttatttga ccgttcaatt tcggcatctt tcatttccgc gatcgaattt ttgatgctga
      121 taatctcttt cgcatcgcct tcgcgaattt tcagagcctc gatctgatcc gttgagtcgg
      181 gcaaaaacgc cagcgtatta accagctcgg tcatttcaga aaattgccga cggaacacac
      241 gagtggtcat gaaatatacc accagcaaca cgataataat gaacgcaaac ggtagataaa
      301 aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag atttcgaaca
      361 atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca gaatcaataa
      421 tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt tgattcttgg
      481 caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg atttgatcaa
      541 gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc gctaacaaat
      601 cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc ggacgatcaa
      661 agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc gaccacggtc
      721 gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca agcggtccat
      781 ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa aagagataat
      841 atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttca
      901 gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg
      961 tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa
     1021 agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atcatgaaaa
     1081 aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct
     1141 tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa cgctgtgccg
     1201 ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag
     1261 cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaaa
     1321 caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc
     1381 ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat
     1441 gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg
     1501 gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa
     1561 tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata tgacgcaata
     1621 aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca
     1681 acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa atggcgactg
     1741 cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa
     1801 tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt
     1861 cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata aacacggcaa
     1921 acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc
     1981 ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat
     2041 caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca
     2101 ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga
     2161 attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacaccg
     2221 cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc
     2281 ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat
     2341 ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta agcgtccaag
     2401 tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa agccaaactg
     2461 taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt tgaggacaac
     2521 ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacgtc
     2581 gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc cgagcgtcag
     2641 ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc agcccagttc
     2701 aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg agatccccga
     2761 caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat
     2821 tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt
     2881 tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc
     2941 aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc
     3001 caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat
     3061 caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca ccgagacgat
     3121 aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca ttccactccc
     3181 tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg catgatccaa
     3241 aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac
     3301 ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt ttccgccgga
     3361 tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg
     3421 gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag tgcccagtcg
     3481 gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc
     3541 cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta
     3601 atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg
     3661 ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat
     3721 tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactt
     3781 ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt caccagcgcc
     3841 ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc
     3901 gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt
     3961 ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca
     4021 ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg
     4081 tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg
     4141 gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa accaatctcc
     4201 ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg ggcaagaata
     4261 ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgcc
     4321 tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc cggatcgctg
     4381 gcccggtcca gattcgtttt taccgccact gcgttgaaat cggtgccatc ctggaattta
     4441 attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt ataactctcc
     4501 gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat
     4561 ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt ggtgaaattc
     4621 gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc
     4681 gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc
     4741 tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg
     4801 cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg
     4861 aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac
     4921 tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcaccgtga
     4981 accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac
     5041 cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag
     5101 caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc
     5161 aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc
     5221 gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag
     5281 caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta cacgcagcgg
     5341 ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa
     5401 aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg tatcgattcg
     5461 ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc
     5521 ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag
     5581 atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct
     5641 gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc catccaccac
     5701 cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata tcaatgggaa
     5761 acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc
     5821 acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc
     5881 ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg
     5941 agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat
     6001 ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg
     6061 gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg
     6121 cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt
     6181 cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat
     6241 ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa atatcatcgc
     6301 catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac
     6361 atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc
     6421 caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgcctacaat
     6481 tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag ctattttctg
     6541 ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac cggcatcaag
     6601 ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat
     6661 cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc
     6721 cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta
     6781 ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt
     6841 catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa accgccacac
     6901 tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta
     6961 atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct aaatccaacg
     7021 ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga
     7081 ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga
     7141 aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc
     7201 caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac
     7261 gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg
     7321 cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg
     7381 gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgccggctt
     7441 ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag cgtaattcct
     7501 gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaatgactg
     7561 ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa
     7621 tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc atgtaaaggc
     7681 aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg acataatagg
     7741 caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg
     7801 ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac ccaggaaacg
     7861 ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccactggat
     7921 gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc cgatattgcc
     7981 tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata ccatggtgaa
     8041 tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga aggctgcgaa
     8101 gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa
     8161 gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg tgcggttgtt
     8221 ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc actggggatt
     8281 gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc
     8341 cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg tctcgccgta
     8401 cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat ccgcgacgat
     8461 ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt agtgatcagt
     8521 tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga agagctgggg
     8581 gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt cggtaaactc
     8641 agcaatagct ggttctgcgg cctgccgggc aacccggttt cagcgacgct gaccttctat
     8701 caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg cctgcccgcg
     8761 cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct tgatttccag
     8821 cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac cggacatcag
     8881 ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct ggaacgcgat
     8941 cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga
     9001 ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc
     9061 tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg
     9121 gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc
     9181 tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag acactgcaca
     9241 gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg acgcggatca
     9301 acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa cttgcagcat
     9361 tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta cgtaatcaac
     9421 tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg
     9481 aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc
     9541 gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg
     9601 gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt tatggcaaac
     9661 cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac
     9721 tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca ggcctgataa
     9781 gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg tgaacgcctt
     9841 atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg cactccgcca
     9901 cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc
     9961 tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat agctaatcat
    10021 ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta agcagtccag
    10081 cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg catgcatttt
    10141 caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag catcaatacg
    10201 attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc cttgtgccgc
    10261 gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct taatcgctgc
    10321 cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag aacgcagctt
    10381 aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa acagacgccc
    10441 ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt ttttacccgc
    10501 ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgcctt
    10561 caccgcaaca acgtctgaag tatccagata cagttccata accatcctca aaatgtactt
    10621 tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcta
    10681 actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta tgattttcaa
    10741 tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat ttcttaaagg
    10801 ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta cgcaggatct
    10861 gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg ccgcgccgga
    10921 agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa ccccggagca
    10981 tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg gtgaagtgaa
    11041 aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg atcgcagcgg
    11101 cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg cgacggcgct
    11161 gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc tacatgtgcc
    11221 gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg atttaaaaca
    11281 cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg tgctggataa
    11341 cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg
    11401 ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg acgagctgca
    11461 cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat atcacttact
    11521 taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc tcgattttgc
    11581 ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc
    11641 acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat
    11701 attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa
    11761 catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca cctggcgaat
    11821 cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag cgaagttcgc
    11881 gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat tgatgatctg
    11941 gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt tctgcatgaa
    12001 gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat gtttaccgat
    12061 gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat gacctccggc
    12121 gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga tggtctgcgt
    12181 gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga tttgcacggc
    12241 gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt
    12301 ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg tcgcgatgaa
    12361 ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg
    12421 caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc taacggtcac
    12481 tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg cgacgttgaa
    12541 ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg ctggttgaaa
    12601 ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc gggaagtccg
    12661 ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc aatggacgcg
    12721 gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc cactcagcct
    12781 aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc ctgcgtacag
    12841 gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt gatcccggaa
    12901 tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat tggttgtata
    12961 gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc
    13021 gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg caaagtgttc
    13081 ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga agtgatggcc
    13141 gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga atatgtcgtc
    13201 gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt ggatgactgt
    13261 attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg
    13321 caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact ggtgtttgaa
    13381 cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt cgatggcctg
    13441 actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat
    13501 gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga actgaaacag
    13561 taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc gggtacgtcg
    13621 tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga cgggcgtaaa
    13681 gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca tctcggccct
    13741 actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg cggcgtgttg
    13801 ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca gaaactgatg
    13861 atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta caacatcgtt
    13921 tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga tctggtagtg
    13981 cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca ggacgatatc
    14041 atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt cgccgggcta
    14101 aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt gtgctctgcg
    14161 tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac ctatgagcgt
    14221 aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa cgtacaacca
    14281 accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt tcgttgtcgc
    14341 cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg atgagatctc
    14401 ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt tccacggcga
    14461 actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata agcaactcaa
    14521 ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg aagcatttgt
    14581 cgcactgatg gcaaaacact accatcgcct gaaacctgta aaatattatc aggagattga
    14641 cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt tagtgatcga
    14701 caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt ttggctttat
    14761 cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac tgaaacgctg
    14821 ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg cggagatgct
    14881 gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc
    14941 ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc aggctgtgct
    15001 ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc tccggttttc
    15061 ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa cttatttata
    15121 acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc gaggtggtta
    15181 tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct atccgtcaga
    15241 tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat cagctaagcg
    15301 atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc gatgccgtct
    15361 ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag cagcgcgaac
    15421 agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa caagcgctag
    15481 gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag gtctacaaag
    15541 gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt taccccatct
    15601 actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat gcgcagtcgt
    15661 ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac ccggatgtga
    15721 gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc aaaggtcttg
    15781 gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat cagcacgttt
    15841 tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg gcacatcgta
    15901 cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt cggacattcc
    15961 agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac gtcgtgccca
    16021 ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg ccggaggatg
    16081 aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg catccactgt
    16141 taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc tggtggcact
    16201 gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc aacgtgatgt
    16261 acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg
    16321 cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa acaaactggg
    16381 aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg ggcatggatg
    16441 tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg acgttactca
    16501 gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt atccggccta
    16561 caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag cgtagcgcat
    16621 caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg gtgacggata
    16681 atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct aaagtcgatc
    16741 acgcgttccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa ctcacggtcg
    16801 tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag tgactcgatg
    16861 gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg cttctgcatc
    16921 attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt agctggcttt
    16981 ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc ctgctcgtca
    17041 tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt ttcaaactca
    17101 tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt tacggtgccg
    17161 ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc gacgccgttg
    17221 gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt tttaaacagt
    17281 ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg gaacagtttc
    17341 ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac ctcttccagt
    17401 ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga ggcgttggcg
    17461 ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt ggcgttatcg
    17521 gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta gttacccgga
    17581 taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat gttaaggaag
    17641 tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac ctgttccagc
    17701 cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag aatatccggc
    17761 tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc aacttcactc
    17821 atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc accggcgcga
    17881 gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc cactttatag
    17941 ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg cttcacttcc
    18001 cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc aaaggcaaac
    18061 tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt acccagcgtc
    18121 ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc gttcgcgcca
    18181 atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa caacggctta
    18241 ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg aaaagagata
    18301 tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg cttaaggtct
    18361 acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg
    18421 aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc ctgccaggca
    18481 caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt
    18541 tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg acgggagtcg
    18601 cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc cgctggagta
    18661 ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc cgggtgtgga
    18721 taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga tcctgccgga
    18781 taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt actatccgaa
    18841 agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca aagatacgcc
    18901 tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga cgccgaccgc
    18961 caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg ttccggcagg
    19021 tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg ctatccatgg
    19081 caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc gtctacgtaa
    19141 cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac agtttattga
    19201 tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag tccataaccc
    19261 gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta ccctgacgaa
    19321 gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg atgaagcgat
    19381 caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta aaacaaaggc
    19441 ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc ataactaaca
    19501 aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga tggaaacgcc
    19561 accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc gatcctggta
    19621 ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga ggtacataat
    19681 tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg actgccgtta
    19741 accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc aacgctggaa
    19801 gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc aacgtccagt
    19861 ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca atcaccagtc
    19921 cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc cccagacaat
    19981 tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat tgcagcgcct
    20041 ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg ccagccagcg
    20101 gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag ataagaatat
    20161 tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc gcctcaaaaa
    20221 taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc agcggaacaa
    20281 caataaacag cgcgacatcg ttggtctgaa aggtagaaag cagcgccgcc gccagcacca
    20341 taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc cccagcacat
    20401 caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt aaggtgatga
    20461 tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg aaaaagctta
    20521 atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa
    20581 aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt cgagcgtctc
    20641 ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc
    20701 caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct gcgccagctt
    20761 ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca gccccatggt
    20821 tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac gagcagccat
    20881 gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct caacgtaatc
    20941 atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa cgtgctcttc
    21001 cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac gactcattgt
    21061 gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca ttgtaaacca
    21121 gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat agcctgtgct
    21181 atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag tcaggaggtc
    21241 ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt
    21301 taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa gataaacctt
    21361 cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca ggcataacgc
    21421 gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc aaaagatttg
    21481 ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc tgcgtatatt
    21541 gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt attgaccctt
    21601 gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt aaccctcaaa
    21661 gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta ctttttacag
    21721 tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac cgctgcgttt
    21781 acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt
    21841 gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg cgcgcctaat
    21901 ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga aatcctgacg
    21961 atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg cgtaatagca
    22021 atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg
    22081 gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg gggtggcgta
    22141 tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt catcgtcaat
    22201 aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat gaaagatacc
    22261 tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat cggtgaaagc
    22321 gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc gtttgccagc
    22381 agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac gcagaaatcg
    22441 cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga taactttgtc
    22501 accctagcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg ccaaatcggc
    22561 gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa
    22621 gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga tatgaccgct
    22681 caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct gatgggctcg
    22741 catccgcagg cctgcgacag gacacaaggc aaatacaaaa cctttgtgca atcgaaagaa
    22801 acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa gctgtacgat
    22861 cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca cggtctggcc
    22921 tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata tcagcaaaat
    22981 ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt gattaaagcc
    23041 cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat taaagcgaag
    23101 gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat atacatcacc
    23161 aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt cgacccgaaa
    23221 ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag tgattagaag
    23281 cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg
    23341 taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag
    23401 aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca
    23461 taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca
    23521 gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt acggcttttc
    23581 tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat
    23641 ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg
    23701 ccagttacgg tagaagtcgc agctacggaa gtacctgcgg tgaaagccag aactgcggcc
    23761 agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct
    23821 aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg
    23881 ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt
    23941 tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa
    24001 tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt
    24061 ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc
    24121 tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca
    24181 atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca
    24241 ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg
    24301 atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg
    24361 ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag
    24421 acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg
    24481 ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg
    24541 ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca
    24601 ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa
    24661 catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt
    24721 ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct
    24781 gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg
    24841 ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg
    24901 atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc
    24961 gccgcttcaa tggggtctac gctgacagta cgcaaagagg gcaaaataaa agaattagac
    25021 attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt ctcgctactt
    25081 ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc
    25141 tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg aatctttatt
    25201 agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga
    25261 acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata
    25321 tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg
    25381 caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat
    25441 ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa
    25501 cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga
    25561 taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca ctcaagttat
    25621 caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct
    25681 gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga
    25741 agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt
    25801 cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt
    25861 acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca
    25921 cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt gtatccacat
    25981 catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta aataatcact
    26041 gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg
    26101 tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca atgagttgca
    26161 aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga
    26221 aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg accctggctt
    26281 ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg
    26341 ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg
    26401 ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca
    26461 ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg
    26521 agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg
    26581 tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg
    26641 tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc
    26701 gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg
    26761 ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca
    26821 aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg
    26881 acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca
    26941 acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac acctgtaaag
    27001 cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc gcaaacccgc
    27061 tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata
    27121 tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa
    27181 tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag
    27241 gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc tttattgaag
    27301 tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc ctgccgatgg
    27361 cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc atgatcaact
    27421 ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac aaaggttttc
    27481 gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac gtcattttac
    27541 cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc agcattaaag
    27601 acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg caagaaatta
    27661 ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc tatctgatta
    27721 ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa atcctgtgat
    27781 tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca atatcgattt
    27841 gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg gtaaatcgac
    27901 cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga ttgtcgatgg
    27961 cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag caggtatggt
    28021 gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca tgtttggtcc
    28081 gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg agctgctggc
    28141 gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg gtggtcaaca
    28201 gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc tgtttgatga
    28261 accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta tgcaggatct
    28321 ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg ccgagaaagt
    28381 agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca atccgcaggt
    28441 gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg tctcttaata
    28501 agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc aacttattaa
    28561 aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa aaaagcccgc
    28621 acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg gaggcacacc
    28681 agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt
    28741 tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca
    28801 cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact
    28861 gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca ctggcggatg
    28921 tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact gtcgccgcga
    28981 cgccccctgc ggaacctgtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg
    29041 tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg
    29101 ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca
    29161 gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga
    29221 ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg caaaaaaatc
    29281 gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt attatcgacc
    29341 tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag
    29401 gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat
    29461 ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca
    29521 cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta agcagcctga
    29581 ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag
    29641 gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct
    29701 tccgcaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggctt
    29761 ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt
    29821 ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa tttatgatgg
    29881 gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt
    29941 cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac tatccggagc
    30001 tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg
    30061 tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc
    30121 tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca ctcattcttt
    30181 tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg
    30241 atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta
    30301 acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg
    30361 tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt
    30421 cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa aacggcatga
    30481 acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc
    30541 gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa
    30601 ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc
    30661 gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg gaattgatgg
    30721 aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg attgtcggct
    30781 taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga
    30841 cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg
    30901 cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct gaaccgctgc
    30961 cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc tgctcgtcgt
    31021 tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta
    31081 ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac
    31141 gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca
    31201 tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc tgaccaccaa
    31261 agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc
    31321 gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt
    31381 ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc agacgaatgg
    31441 cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc
    31501 tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac
    31561 agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt
    31621 cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga
    31681 gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat
    31741 tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct gcgggtgtaa
    31801 gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc
    31861 tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta
    31921 agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat
    31981 tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg
    32041 catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa gcatatctct
    32101 gtcaattttt tgcgtcattt gcatcgcgca ggcaacctac acttatcagg cactacccgt
    32161 tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg
    32221 agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag
    32281 ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat
    32341 aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc
    32401 ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc
    32461 attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc
    32521 aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gtcaagtgat
    32581 aatgcttatc aaaattatta tcacttgcac aaacactatc acgggattaa cagtggcatc
    32641 gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc
    32701 tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt
    32761 gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga agggcaagct
    32821 aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa
    32881 tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt
    32941 tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgtc
    33001 cccggcg
//
LOCUS       DADMFL010000037        32690 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.037, whole genome shotgun sequence.
ACCESSION   DADMFL010000037 DADMFL010000000
VERSION     DADMFL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.037"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..223
                     /locus_tag="JNZ27_001706"
     CDS             <1..223
                     /locus_tag="JNZ27_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8664072.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            397..1167
                     /locus_tag="JNZ27_001707"
     CDS             397..1167
                     /locus_tag="JNZ27_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664073.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCKDGISITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSV
                     KNGESTFTASANERASKVLKSGNALRKYSVNPNKKGTY"
     gene            complement(1200..2720)
                     /locus_tag="JNZ27_001708"
     CDS             complement(1200..2720)
                     /locus_tag="JNZ27_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8664074.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2811..3530
                     /gene="tonB"
                     /locus_tag="JNZ27_001709"
     CDS             2811..3530
                     /gene="tonB"
                     /locus_tag="JNZ27_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY8664075.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="JNZ27_001710"
     CDS             complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="JNZ27_001710"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY8664076.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4073..4612)
                     /locus_tag="JNZ27_001711"
     CDS             complement(4073..4612)
                     /locus_tag="JNZ27_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY8664077.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4642..5385)
                     /locus_tag="JNZ27_001712"
     CDS             complement(4642..5385)
                     /locus_tag="JNZ27_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY8664078.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5413..5502)
                     /locus_tag="JNZ27_001713"
                     /pseudo
     CDS             complement(5413..5502)
                     /locus_tag="JNZ27_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5742..6380
                     /gene="ompW"
                     /locus_tag="JNZ27_001714"
     CDS             5742..6380
                     /gene="ompW"
                     /locus_tag="JNZ27_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY8664079.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6440..6946)
                     /locus_tag="JNZ27_001715"
     CDS             complement(6440..6946)
                     /locus_tag="JNZ27_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8664080.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(6992..7492)
                     /locus_tag="JNZ27_001716"
     CDS             complement(6992..7492)
                     /locus_tag="JNZ27_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8664081.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7578..7757)
                     /locus_tag="JNZ27_001717"
     CDS             complement(7578..7757)
                     /locus_tag="JNZ27_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY8664082.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="JNZ27_001718"
     CDS             complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="JNZ27_001718"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY8664083.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="JNZ27_001719"
     CDS             complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="JNZ27_001719"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY8664084.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10149..11507)
                     /gene="trpCF"
                     /locus_tag="JNZ27_001720"
     CDS             complement(10149..11507)
                     /gene="trpCF"
                     /locus_tag="JNZ27_001720"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY8664085.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="JNZ27_001721"
     CDS             complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="JNZ27_001721"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY8664086.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="JNZ27_001722"
     CDS             complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="JNZ27_001722"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY8664087.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            14941..15822
                     /gene="yciV"
                     /locus_tag="JNZ27_001723"
     CDS             14941..15822
                     /gene="yciV"
                     /locus_tag="JNZ27_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY8664088.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15819..16439
                     /locus_tag="JNZ27_001724"
     CDS             15819..16439
                     /locus_tag="JNZ27_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY8664089.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16540..17412
                     /gene="rluB"
                     /locus_tag="JNZ27_001725"
     CDS             16540..17412
                     /gene="rluB"
                     /locus_tag="JNZ27_001725"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY8664090.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="JNZ27_001726"
     CDS             complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="JNZ27_001726"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY8664091.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18039..18797)
                     /locus_tag="JNZ27_001727"
     CDS             complement(18039..18797)
                     /locus_tag="JNZ27_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY8664092.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19017..20066
                     /gene="sohB"
                     /locus_tag="JNZ27_001728"
     CDS             19017..20066
                     /gene="sohB"
                     /locus_tag="JNZ27_001728"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY8664093.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20102..20353)
                     /locus_tag="JNZ27_001729"
     CDS             complement(20102..20353)
                     /locus_tag="JNZ27_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY8664094.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEAIGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20733..23354
                     /gene="topA"
                     /locus_tag="JNZ27_001730"
     CDS             20733..23354
                     /gene="topA"
                     /locus_tag="JNZ27_001730"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY8664095.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLI
                     DPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACT
                     NEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSR
                     ETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKA
                     TGWSAFYVDGKWVEGKK"
     gene            23564..24538
                     /gene="cysB"
                     /locus_tag="JNZ27_001731"
     CDS             23564..24538
                     /gene="cysB"
                     /locus_tag="JNZ27_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY8664096.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24833..24997
                     /locus_tag="JNZ27_001732"
     CDS             24833..24997
                     /locus_tag="JNZ27_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY8664097.1"
                     /translation="MLISDGDYMRLAMPSGNQEPHRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            25000..25167
                     /locus_tag="JNZ27_001733"
     CDS             25000..25167
                     /locus_tag="JNZ27_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664098.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25281..25376
                     /locus_tag="JNZ27_001734"
     CDS             25281..25376
                     /locus_tag="JNZ27_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664099.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25540..28134
                     /gene="acnA"
                     /locus_tag="JNZ27_001735"
     CDS             25540..28134
                     /gene="acnA"
                     /locus_tag="JNZ27_001735"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY8664100.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAESFERIHRSNLIGMGILPLEFPQGVTRKTL
                     GLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCRIDTATELTYYQNDGILH
                     YVIRNMLK"
     gene            complement(28198..28788)
                     /gene="ribA"
                     /locus_tag="JNZ27_001736"
     CDS             complement(28198..28788)
                     /gene="ribA"
                     /locus_tag="JNZ27_001736"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY8664101.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            28958..29722
                     /gene="pgpB"
                     /locus_tag="JNZ27_001737"
     CDS             28958..29722
                     /gene="pgpB"
                     /locus_tag="JNZ27_001737"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY8664102.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29871..30179
                     /gene="lapA"
                     /locus_tag="JNZ27_001738"
     CDS             29871..30179
                     /gene="lapA"
                     /locus_tag="JNZ27_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY8664103.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30186..31355
                     /gene="lapB"
                     /locus_tag="JNZ27_001739"
     CDS             30186..31355
                     /gene="lapB"
                     /locus_tag="JNZ27_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY8664104.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31548..32285
                     /gene="pyrF"
                     /locus_tag="JNZ27_001740"
     CDS             31548..32285
                     /gene="pyrF"
                     /locus_tag="JNZ27_001740"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY8664105.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32285..32611
                     /gene="yciH"
                     /locus_tag="JNZ27_001741"
     CDS             32285..32611
                     /gene="yciH"
                     /locus_tag="JNZ27_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY8664106.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         8281 a         7894 c         8328 g         8187 t
ORIGIN      
        1 tccacagttt tcacctgaag cattaaataa tctgaaaggt cgagtggaaa cattcacaca
       61 agatttaaag gttatcaaac cgtcagtaaa tattcagcct cagacaatca atatcacaac
      121 tcagacggta cttgatggaa aggttattga tgaaagaacc acatctcata ttaatcgtat
      181 gcaggaagat accttgatta gctcagctta cccagaggaa taaacaatgt tagaactact
      241 aatatttatt gccacacatg gcaatctatt aatctttatc tagtatgacg attcaactat
      301 gaatagtcga atgctacgtg atttcttctg agcagatacc aacatcaaaa attagatttc
      361 acgtagctca cgcttaacag aataggaata acaacaatgt ttgacacaaa gcaaattaga
      421 tactcaggta aagatggtat ctattttcac cttagagata atgtagaatc ttttcttgcc
      481 ttaacagcta ctgaacagat ggactatgac tcaccgatga tggtgacaac tcaggctatg
      541 caatcggggc aaactatcac tgataatgtt cagagagcac ctaagaccat ctctattagc
      601 ggtgttgtag tggttgattc gacgggttca gttctattaa ccagacaggg gcaacttgta
      661 gagaccttta tttctacggt tgaacaatgg cgagaccaga agcaaatcat cactattgtt
      721 tgtaaagatg gaatatctat cactgatagt atcattactc agtttaaagc gagtaaagag
      781 cataccattg ctaatggtct tcgtattcag atgaccttcc aaggtgtaga atttcgttca
      841 atcgtagggc aaactgatat ttcagcagct actggcaaga ccgctaccac gaatgatggt
      901 ggtgctacca gtaaaaagaa cactggcaac actacaacga gtcttggaag ccctatgctg
      961 acctgtaaag agctttttag ctattcggca agtgagttat cagatgaagc gctaaaagcc
     1021 cgtgtaacgt gctctaagag cgttagcgtt aaaaatggtg agagcacctt cacagcctca
     1081 gctaatgagc gagcttcaaa agtgcttaag tcgggtaatg ctttacggaa gtactctgtg
     1141 aatccgaata agaagggcac ttattgagac ataaaaaggg gcttcatagc ccctgaaaat
     1201 tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa gattggtgac
     1261 ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt ctgtagcgtt
     1321 atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa aattaattga
     1381 cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg tagttcctta
     1441 tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc tgtgcgcttc
     1501 tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc aagaagtagg
     1561 gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa cgctgcaatc
     1621 ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt atcaatcatc
     1681 catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc taagtggtta
     1741 ttgcacctgt agaccccagc atacttattc tttgttccgg taggatggac actagcccca
     1801 cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc acttgcaggt
     1861 gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt tgcagcatct
     1921 atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga tgctggtagt
     1981 gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct aaatccatta
     2041 gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg tgcaatgtta
     2101 tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc agcatgttca
     2161 ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa acgttgttca
     2221 ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa agacttctgt
     2281 ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat tcctaaatca
     2341 tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat ctcgatacca
     2401 tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg gcctatgcgg
     2461 tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc aatgcgggag
     2521 agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag accttcatca
     2581 ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt gaatagctca
     2641 ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga gaatctcaag
     2701 tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca tttaaagatg
     2761 aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca atgccccttg
     2821 atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat ggtgctgttg
     2881 tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg cctgcgcaac
     2941 caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc gttcagccgc
     3001 caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa ccgccaaaag
     3061 aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg aagccggtga
     3121 aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt ccggcatcac
     3181 cgtttgaaaa tacggcaccc gcgcgcccca catcaagtac ggcaacggct gcaaccagca
     3241 agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag ccgcagtatc
     3301 cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt gacgtcacgc
     3361 cggatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac atgtttgagc
     3421 gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca ggcagtggga
     3481 ttgtggtgaa tatcctgttt aaaattaacg gcaccaccga aattcagtaa gcagaaagtc
     3541 aaaagcctcc gaccggaggc ttttgactat tactcaacag gtaaggcgcg aggttttcct
     3601 tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg ttgcccgatt
     3661 ggttcagacg ctactttttt cacccacact tcaatattaa tgctgaccga tgtcgtccct
     3721 ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg ccgtaagaaa
     3781 gtcattcctt caacccgcac agtcactacg cgaccgtggg caatttcttt tgccagaata
     3841 gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc attggcattg
     3901 gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac gttatgtgtt
     3961 gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac tatgattttc
     4021 ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct ggttaggatt
     4081 tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg aagattaagg
     4141 taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc agccagaatg
     4201 cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc agatttagct
     4261 tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga attagcggct
     4321 ttttcatcac ccattggctg actaacaggg ctaccgcaaa cagagcataa atgactgtaa
     4381 ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg aagacgacca
     4441 ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc cagctataga
     4501 taagcacaat cgccgtggcg acgatcagcg ctgcagtagc cgcatagatg tcataaatct
     4561 tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc attttacgat
     4621 tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga acagatagat
     4681 gagcaatatg gcagaaatca ggttgctcaa ggtgttcgcc agtacggcac caatttccgg
     4741 ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca gcaaccagct
     4801 cagtactgcg ggtgccacca gacgcatatt cgcccaagtc agccgcatac tgctacgcat
     4861 cgaggcaaaa atgcccattt tgtcctgtac cagcatcacc ggtgccaggg ccagtaaaat
     4921 ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta aaagggtagt
     4981 cagaaaaatc agaataaata acttgggcaa tatcggcgca ctggcaccaa tagctcgcag
     5041 cgcacagact ctctgacccg cagacaccag ctggataatt aatattacgc ctccggcgag
     5101 aatggcgtta ccgattaatt ctgaaaaagt ggacgccgct gaagcctgca gcaaaatttg
     5161 ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc cgctaacggg
     5221 cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat gccctaacac
     5281 cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt gattacggaa
     5341 gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact ctccttgagt
     5401 tttatgcagg tgttaattag cgggcaattg taccctggtt aagcctcggg tggcagcatc
     5461 aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc cttcaggcgt
     5521 gggcaaaccg cggcaagtgg cgatcgagtt aatatttgcg tagagaaaat atttaaaaat
     5581 tgatttaaat cacattaacc aggattctca atgcaacttc taaattaatc cagatcaata
     5641 aatggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact ccaatgtagg
     5701 tatattcgtc acgtttttat aaccataacg acggagcgga tatgaaaaag ttaacagtgg
     5761 cggctttggc agtaacaact cttctttctg gcagtgcctt tgcgcatgaa gcaggcgaat
     5821 tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt agtacgttag
     5881 gaagtctggg tggattcagc gtgaccaata acactcaact gggcttgacg tttacttata
     5941 tggcgaccga caacattggt gtggaattac tggcagcgac gccgttccgc cataaaatcg
     6001 gcacccgggc gaccggcgat attgcaaccg ttcaccatct gccgccaaca ctgatggcgc
     6061 agtggtattt tggtgatgcc agcagcaaat tccgtcctta cgttggggcc ggtattaact
     6121 acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcgggg ctttccgatc
     6181 tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat ctgattaacc
     6241 gtgactggtt ggttaacatg tcagtgtggt acatggatat cgataccacc gccaattata
     6301 agctaggcgg tgcacagcaa cacgatagcg tacgcctcga tccgtgggtg tttatgttct
     6361 cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg gggtcaataa
     6421 ggacatggta taaagggtat tatttcttcg cttctacgcc atcagtttca gaacgaatta
     6481 aaaatttctc agttgtttgc ggaatatgct gaatcagcca gtcggccatt tgcttttcct
     6541 catttaaaat cgcttcgatg atcggaattg agactgtatc accggcattt tttgctgctg
     6601 ctaatagtga ggtgtaacag gcgatttcaa attgctcgaa gacatatccg ctaatagagc
     6661 ctttaactat ttcatcagaa gggaatatac caccgattga ctgcccaagc gcagccattt
     6721 tactcatgga atctttaatg actgaacgtg aaatgtcatt acgatcaaga atagtttcca
     6781 gttgaacaat ctggtttttg gtttcactaa gatgttgttc aatacgagcg cgtagttcag
     6841 gataattatc tatacggctg gccatggatt caagcataga ttcggcttgc ttttccattg
     6901 cgtgggcgtc acgtaaccag tcatgataat gttcaatacg attcatgaaa atgtcctctt
     6961 tataaaatga ctaaaagtta aaattcatat ttcaggcttt attttcggct ttcttattta
     7021 cgttattaag ggccagatca gtcagtttga tgtcggtggc cttttcttct tccagggttt
     7081 ctttcagaag cttcgctgct ttacggtaac ctaattgttc agccagcgtc gctaatgtcc
     7141 cgtaactggc aatctcataa tgctcgactt tctgtgctgc ggcaatcagt gcggcatcac
     7201 gcacttcgtt tttctcggta ctttcgatga cctcattagc ttcttcaata agaccttcca
     7261 ttgccacaca tttcatgcgc ttaattttca gatttgattc tgattccaca acttgatcaa
     7321 ttcgttcaat ctgtccatga gtttcctcga ggtgcgcatg aaaagcctga cttaattttt
     7381 catttgatgt tgctcttgcg agttttgcca gtgcccgggg taattgtttt tctgcgctgt
     7441 aggtatctga aagcagttga ataaatacat cttcaatggt cttcatattc atatttttct
     7501 ccagtgaaat cactgcgagc catacggccc gcagatgcaa ttatctttat cagctgatat
     7561 taataattaa cggtgaatca ggatttgtct gatttattac caccgctttg ttgaccgcct
     7621 tttttacccg cttcagatgc gcgttgcgga tcatttttaa aattaccgcc gctatgctga
     7681 ccgcctttac ggcctgcgtc ggatgccttc tcacggtctt cggcgaaatt tcctgaacca
     7741 ccacgatgtt cggccatgtt atttctcccg ttgcgttgca ttgtttcatt aatatgagtg
     7801 ttgtgtgtcg acactcatta aaattagtcg ctaatgagaa ttagtcaaat taagcgcaac
     7861 gagaagatag agggaaaata tattttgagg aacattctgg atatattaac aattacctga
     7921 ggaataaatg acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt
     7981 gtttgattat attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag
     8041 cgaaacggta aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa
     8101 agttaaaatg ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca
     8161 tcggttgtac aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct
     8221 cgatgatttt aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg
     8281 cttttacctg atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact
     8341 ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca
     8401 cgcccgctcg tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagca
     8461 ggtcgtcatc ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg
     8521 cctggcggaa gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc
     8581 cgactttctc gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat
     8641 acatcaacag gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa
     8701 aacattgtgc cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg
     8761 ggccatccgc cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt
     8821 caattagcgt atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga
     8881 aaggaacgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt
     8941 ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc
     9001 gcgaccggaa aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt
     9061 cagcgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca
     9121 cagcgttttg aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc
     9181 agtgctgtta agatacgcgt gttgtgggcc gacagacggg aaatccagtc cggcggagat
     9241 ggagtaagat tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa
     9301 ataaatgccc acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg
     9361 accaccaggc tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat
     9421 gccgatggca ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc
     9481 tctttccaga atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat
     9541 ggtcggataa ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact
     9601 gccggaccag tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg
     9661 taccgggatc acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc
     9721 aacgtctttg gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc
     9781 cgacgccacg ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg
     9841 cttcgccagc aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa
     9901 atcttcacgc ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt
     9961 cagtgcggtt ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc
    10021 aggatctttt tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat
    10081 ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt
    10141 tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg
    10201 atgcccggtt gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc
    10261 gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga
    10321 ccatttaata gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca
    10381 tatttatcaa cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc
    10441 cagatggcaa catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca
    10501 ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca
    10561 atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc
    10621 gcctgttcaa cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc
    10681 gcgcccgcgt cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc
    10741 tcacccagca acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa
    10801 ccaatcagaa aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg
    10861 ccggattcgc tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg
    10921 ttgagatcaa tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct
    10981 aatgcaatgg cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc
    11041 agactgtgag cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt
    11101 aataagcagg catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg
    11161 aagtctttac ataaaattgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag
    11221 ctcccctgaa aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa
    11281 atggcggcaa tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct
    11341 tttttacact ccagaataaa cgccgtgcgt gcaccctgta gcgcatcata aaaatgtcgt
    11401 gtgctcggct gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct
    11461 acccaaatcg ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt
    11521 gcagccagcg cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt
    11581 tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca
    11641 gcgacggctg cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca
    11701 cggttttctt caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa
    11761 tcttcagcgg tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc
    11821 ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac
    11881 cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt
    11941 aacgccagcg gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg
    12001 gttttcagtt gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca
    12061 aagaggaaac atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca
    12121 agattaatac cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg
    12181 ttaccgtgtt tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa
    12241 atattgatac tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc
    12301 gggcgcggga acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc
    12361 gggtgctcac cgcgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc
    12421 tcgccacgca ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc
    12481 tgatacagtt tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc
    12541 caggccagcg tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg
    12601 aactggaatc cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa
    12661 tgggcgttga tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc
    12721 accggcagcg ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac
    12781 gctttaccgt gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt
    12841 gcctgatgtc cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt
    12901 tccggcatac aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga
    12961 ttgctcatcg tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc
    13021 accacgttat gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata
    13081 ttatcgagca gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa
    13141 tagcgcgcag tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag
    13201 aatcaaggac tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg
    13261 agcggatcac aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc
    13321 cgtagctgcc gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg
    13381 gcgcaccgct taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat
    13441 cgtgacgcag ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa
    13501 ctttggtgag atcggcgacg tagcggctgc caggggtgca aatgcgtgcc agatcattac
    13561 gggcgagatc aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca
    13621 gttcaatacg gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc
    13681 gtgttccggc aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc
    13741 tttccggcga cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc
    13801 tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga
    13861 aacggcgaga tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac
    13921 gcactacgcc accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg
    13981 aaaccactgg cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag
    14041 cagtgagacg ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc
    14101 tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc
    14161 agttattttc cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga
    14221 acaggccgcc gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca
    14281 ataaacggaa agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg
    14341 gactgacagg ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg
    14401 caggcagggc gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa
    14461 ttgtgacggt gtcacctaat gctgtaatgt gcagcgcact gtctatcagc agcaggcttt
    14521 ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc
    14581 cacacaactg gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca
    14641 gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc
    14701 cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt
    14761 caggaagtgg ccaccaacct ttcagtacga aaattgcttt cattgtcgat accattttta
    14821 cgtgaacttg cgtactagtc aactggttcg atgattaatt gtcaacagct catttcagaa
    14881 tatttgccag acccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc
    14941 ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac agcttccgat
    15001 ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg
    15061 gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga aatttcacgt
    15121 tcgggactgg cgctgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat
    15181 gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc
    15241 gcgcagcaga cagaacggcg caatcagcgg gcgcagcaaa ttgctgaaag acttgaaaaa
    15301 gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg
    15361 cgcggtcatt ttgcgcgttt ccttgtggag tgcggcaaag ccagttcaat ggcagatgtc
    15421 tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata
    15481 gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg
    15541 cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac
    15601 ggcgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgcac ccagctggcc
    15661 acacttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccatgc
    15721 ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg cgtctggcag
    15781 ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt ttttatattc
    15841 atcctgataa cccgcagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg
    15901 gggtgattgt ctatccaact gattccggct atgcgctcgg ctgtaaaagt gaagataaaa
    15961 acgccatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga
    16021 tgtgtcgcga tctttctgaa ctctcgacct attcattcgt ggataacgtt gcgtttcgtt
    16081 tgatgaaaaa caacacgccg ggcaactata ccttcatcct gaaggggacg aaggaagtgc
    16141 cacgccgtct gttgcaggag aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga
    16201 ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg ctaatgctgc
    16261 caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg gaaaaacagg
    16321 tagatttgat tattcacggc ggctatctcg ggcagaaacc aacaacggtt attgatctta
    16381 ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag
    16441 tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca ttgattctcc
    16501 tgttcgacgc ctgtgaaggc gacaccaaaa ggaagctcta tgagcgaaaa attacagaaa
    16561 gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt
    16621 cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg
    16681 ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt
    16741 cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg
    16801 cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt
    16861 cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac
    16921 cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag
    16981 gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca
    17041 gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact
    17101 ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg
    17161 agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt
    17221 ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt ggagcttccg
    17281 ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggctaatcag
    17341 attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcgggcgt
    17401 aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag attaataatc
    17461 aatccctatt tgcgccttaa caccggcatc aaacgcatgt ctgatggggc gcagctcact
    17521 taccgtatcc gccagttcaa gaatatcccg atgacagcct cgacccgtga taatcaccgt
    17581 ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata
    17641 cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat
    17701 ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt
    17761 atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt
    17821 gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcccactt tttttccgtg
    17881 accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt
    17941 aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc
    18001 tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta
    18061 cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc
    18121 cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg
    18181 aaggcgcttg cgcgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg
    18241 cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct
    18301 gcatatgcac cccagttggc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt
    18361 gaaccggcgt ccgatttgag taataaagga agcagtgcct gggtgagcat aaaggtggca
    18421 ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa
    18481 acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata attaacgaca
    18541 atgcgctgtg ccagttgttg gcaattttcg gacgtgcagg tcagcaaatc gagaataaac
    18601 cactgtggct gacgcccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct
    18661 tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca
    18721 cgaccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt
    18781 tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact
    18841 ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata
    18901 acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct
    18961 taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg
    19021 aattgttgtc tgaatatggt ttgtttttgg ctaaaatcgt taccgttgtg ctagcgattg
    19081 cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt
    19141 tacgggtcaa caatctcagc gaacagtata aggagatgaa agaagaactg gccgcggcgc
    19201 tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag
    19261 aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc
    19321 gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg
    19381 aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg
    19441 aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc
    19501 gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca
    19561 tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg
    19621 gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg
    19681 aactgcacac cgccgggcag tataagcgca cgctgacctt gctgggtgaa aataccgaag
    19741 aagggcggga gaaattccgc gaagagctga acgaaacgca tcagttattt aaagattttg
    19801 tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg
    19861 gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc
    19921 ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca
    19981 ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc
    20041 agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc
    20101 tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa
    20161 acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa
    20221 ataccagcac gccattacgc tgcgtgacgc cgatggcttc aatccctgcg agcgtatcct
    20281 catgctcacg aatgattttg ttagcctctt tcaacagcgt ttcgcggtcg atgggttgtg
    20341 tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg
    20401 cagacaaata taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg
    20461 ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca
    20521 atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac
    20581 ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggg tgatatccgc
    20641 agagagcgag tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca
    20701 ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt
    20761 ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca
    20821 gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact
    20881 ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc
    20941 gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctggtaaag
    21001 agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa
    21061 ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg attggtggtg
    21121 atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg
    21181 catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct
    21241 ttatggaccg cgtggtgggg tatatggttt cgccgctgct gtggaaaaag atcgctcgtg
    21301 gtctgtctgc cggtcgtgtg cagtcggtgg cggtccgcct ggtggtagag cgtgagcgtg
    21361 aaattaaagc gttcgtaccg gaagagttct gggaagtcga tgccagcacg actacgccat
    21421 ctggtgaagc gttggcgttg caggtgactc atcagaatga caaaccgttc cgtccggtca
    21481 acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg
    21541 aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc
    21601 aacaagctgc cagcacccgt cttggttttg gcgtgaaaaa aaccatgatg atggcgcagc
    21661 gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg
    21721 acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg
    21781 aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc
    21841 cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac
    21901 tgtaccagct aatctggcgt cagttcgttg cctgccagat gaccccagcg aaatatgact
    21961 ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc
    22021 gctttgatgg ctggacaaaa gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct
    22081 taccggcagt cgataaaggc gatgctctga cgctcgttga actaacacca gcccagcact
    22141 ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagaactg gaaaaacgtg
    22201 gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctatg
    22261 tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc
    22321 ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg gaaaacagcc
    22381 tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg
    22441 atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga
    22501 accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca
    22561 cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt
    22621 gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgccg gaaaacgaag
    22681 tgctgaacgt gctggaaggc gaagatgctg aaaccaacgc gctgcgcgca aaacgtcgtt
    22741 gcccgaaatg cggcacggcg atggacagct atctcatcga tccgaaacgt aagttgcatg
    22801 tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggcgaa ttccgcatta
    22861 aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ctctgaaatg cacctgaaaa
    22921 tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga
    22981 ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc ggtgccatta cctgagctgc
    23041 cgtgcgaaaa atcagatgct tatttcgtgc tgcgtgacgg tgctgccggt gtgttcctgg
    23101 ctgccaacac tttcccgaaa tcgcgtgaaa cgcgtgcgcc actggtggaa gagctttatc
    23161 gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca cagcaggatc
    23221 cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa tatgtctctt
    23281 cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc aaatgggttg
    23341 aaggaaaaaa ataaccttta attctgtcag gtttttataa acaaagggtc gcgaaagcgg
    23401 ccctttttta ttgcatatta ttttttcttc acacctatac actaaggcta taaatgatat
    23461 agtggttata gttagcacct tttttattat taaatcgtat tagtcacccg ccaggtgtga
    23521 cgaaaaaacg atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact
    23581 tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact
    23641 ttacacatca caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat
    23701 tcaaattttt tcccgaagcg gcaagcacct gacgcaggta acgccagcag gacaagaaat
    23761 aattcgtatc gctcgcgaag tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggtga
    23821 gcacacctgg ccggataaag gctcgctgta tatcgccacc acgcataccc aggcacgcta
    23881 cgcgttacca aacgttatca aaggctttat tgagcgttat ccccgcgttt ctttgcatat
    23941 gcaccagggc ccgccgacac aaattgctga tgccgtctct aaaggcaatg ccgatttcgc
    24001 tatcgccaca gaagcgctgc atctgtatga agatttagtg atgttaccgt gctaccactg
    24061 gaatcgggct attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat
    24121 tgaagaactg gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga
    24181 actggatact gcctttaacc gcgcagggtt aacgccgcgt atcgttttca cggcaacgga
    24241 tgctgacgtc attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat
    24301 ggcggtggat ccggtcgccg atcccgacct tgtgcgcgtt gatgctcacg atatcttcag
    24361 ccacagtaca accaaaattg gttttcgccg tagtactttt ttgcgcagtt atatgtatga
    24421 tttcattcag cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt
    24481 gcgctccaat gaagaaattg aggtcatgtt taaagatata aaactgccgg aaaaataatt
    24541 tcttgcgtta ttttcggcac cttttatgta gcgaaggtgc cggaatatat tctcttttgt
    24601 tacttatttc tataccgtgc acaatatttt tatccctcat atttataggg taaattacct
    24661 gtaaagtagt gtctaattac ccgcctcgca aattccagtt ttcaaatatc tatttcaggt
    24721 caaaagattg aattattcac tggagacgat tcgtaaattc gctggatttt tgactaaagt
    24781 ttctttagga tttgtctcat cgtatgatta attacaccaa tcattcaacg tcttgctgat
    24841 aagtgatggc gattatatga ggttagcaat gccttctgga aatcaggaac cccaccgtga
    24901 tcctgaatta aaacgtaaag cctggctggc ggtttttctt ggttctgcac ttttctgggt
    24961 ggttgtcgca ctgctgattt ggaaagtgtg gggataacta tggtcggtca ggagcaactg
    25021 gagtcgtcac cattatgcca gcatagtgac aatgaaccag aaacgaaacg ggaatgttcc
    25081 gtcgttattc cagacgactg gcaactaaca tcgcagcagc aagcctttat agaactgttt
    25141 gctgaagatg atcagccgaa acaataatta tcatcattct tattacccat ttttaatgaa
    25201 ttaaagggct tttaatacac cgcagcaata acagcttgag ttatctcaac acaaaataat
    25261 aaccgttaag ggtgtagcct atgatcaaca caaatatgaa atattggtcc tggatgggcg
    25321 cgttttctct gtcgatgctc ttctgggccg aactcctctg gatcattact cactgatcct
    25381 tgaccccgct gcggcggggt tgtcatttgc tttgccacaa ggtttctcct cttttatcaa
    25441 tttgggttgt tatcaaatcg ttacgcgatg tttgtgttat ctttaatatt caccctgaag
    25501 agaatcaggg cttcgcaacc ctgtcattaa ggaggagcta tgtcgtcaac cctacgagaa
    25561 gccagtaagg acacgttgca ggccaaagat aaaacttacc actactacag cctgccgctt
    25621 gctgctaaat cactgggcga tatcacccgt ctacccaagt cactcaaagt tttgctcgaa
    25681 aacctgctgc gctggcagga tggtaactca gttaccgaag aggatatcca cgcactggcg
    25741 ggatggctga aaaatgccca tgctgaccgt gaaattgcct accgcccggc aagggtgctg
    25801 atgcaggact ttaccggcgt acctgccgtt gttgatctgg cggcaatgcg cgaagcggtt
    25861 aaacgcctcg gtggcgatac tgcaaaggtt aacccgctct caccggtcga cctggtcatt
    25921 gaccactcgg tgaccgtcga tcgttttggt gatgatgagg catttgaaga aaacgtacgc
    25981 ctggaaatgg agcgcaacca cgaacgttat gtgttcctga aatggggaaa gcaagcgttc
    26041 agtcggttta gcgtcgtgcc gccaggcaca ggcatttgcc atcaggttaa cctcgaatat
    26101 ctcggcaaag cagtgtggag cgaattgcag gacggtgaat ggattgctta tccggataca
    26161 ctcgttggta ctgactcgca caccactatg atcaacggcc ttggcgtgct ggggtggggc
    26221 gtgggtggga tcgaagcaga agccgccatg ttaggccagc cggtttccat gcttatcccg
    26281 gacgtagtgg gctttaaact taccggaaaa ttacgtgaag gtattaccgc cacagacctg
    26341 gttctcactg ttacccaaat gctgcgcaaa catggcgtgg tggggaaatt cgtcgaattt
    26401 tatggtgatg gtctggattc actaccgttg gcggatcgcg ccaccattgc caatatgtcg
    26461 ccagaatatg gtgccacctg tggcttcttc ccaatcgatg cagtaaccct cgattacatg
    26521 cgtttaagcg ggcgcagcga agatcaggtc gagttggtcg aaaaatatgc caaagcgcag
    26581 ggcatgtggc gtaacccggg cgatgaaccg atttttacca gtatgttaga actggatatg
    26641 aatgacgttg aagcgagcct ggcagggcca aaacgcccac aggatcgcgt tgcactgccc
    26701 gatgtaccaa aagcatttgc cgccagtaac gaactggaag tgaatgccac gcataaagat
    26761 cgcctgccag tcgattatgt tatgaacgga catcagtatc agttacctga tggcgctgtg
    26821 gtcattgctg cgataacctc gtgcaccaac acctctaacc caagtgtgct gatggccgca
    26881 ggcttgctgg cgaaaaaagc cgtaactctt ggcctcaagc ggcaaccgtg ggtcaaagcg
    26941 tcgctggcac cgggttcgaa agtcgtttct gattatctgg caaaagcgaa actgacaccg
    27001 tatctcgacg aactggggtt taaccttgtg ggatacggtt gtaccacctg tattggtaac
    27061 tctgggccgc tgcccgatcc tatcgaaacg gcaatcaaaa aaggcgattt aaccgtcggt
    27121 gcggtgctgt ccggcaaccg taactttgaa ggccgtatcc atccgctggt taaaactaac
    27181 tggctggcct cgccgccgct ggtggttgcc tatgcgctgg cgggaaatat gaatatcaac
    27241 ctggcttctg agcctatcgg ccatgatcgc aaaggcgatc cggtttatct gaaagatatc
    27301 tggccatcgg cacaagaaat tgcccgtgcg gtagaacaag tctccacaga aatgttccgc
    27361 aaagagtacg cagaagtttt tgaaggcaca gcagagtgga aggaaattaa cgtcacacga
    27421 tccgatacct acggttggca ggaggactca acctatattc gcttatcgcc tttctttgat
    27481 gaaatgcagg caacaccagc accagtggaa gatattcacg gtgcgcggat cctcgcaatg
    27541 ctgggggatt cagtcaccac tgaccatatc tctccggcgg gcagtattaa gcccgacagc
    27601 ccagcgggtc gatatctaca aggtcggggt gttgagcgaa aagactttaa ctcctacggt
    27661 tcgcggcgtg gtaaccatga agtgatgatg cgcggcacct tcgccaatat tcgcatccgt
    27721 aatgaaatgg tgcctggcgt tgaagggggg atgacgcggc atttacctga cagcgacgta
    27781 gtctctattt atgatgctgc gatgcgctat aagcaggagc aaacgccgct ggcggtgatt
    27841 gccgaatcgt ttgaacgaat tcaccgttcg aatttaattg gcatgggcat cctgccgctg
    27901 gaatttccgc aaggcgtgac gcgtaaaacg ttagggctaa ccggggaaga gaagattgat
    27961 attggcgatc tgcaaaatct acaacctggc gcgacggttc cggtgacgct tacgcgcgcg
    28021 gatggtagcc aggaagtcgt accctgccgt tgtcgtatcg acaccgcgac ggagttgacc
    28081 tactaccaga acgacggcat tttgcattat gtcattcgta atatgttgaa gtaacaagta
    28141 tttgcttgcc ggttattttg cttccggcaa gcaaatgaat tacacaatgc aagagggtta
    28201 tttgttcagc aaatggccca ttttctcggc tttggtatcg agataatgtt cgttattggg
    28261 gttacgacct acaatcaatg gtacgcgttc aacaatatta atccctgctt cggtcagaat
    28321 ttcgactttt ttcgggttat tggttaacaa gcggacttca ttgacgccaa ggagtttgaa
    28381 catatcagcg caaagagtga agtcgcgctc atcagcggcg aagcctaact ggtggttagc
    28441 ctctacggta tcgtaacctt gatcctgcag tgcgtaagcg cggattttat tcagcagacc
    28501 aatgttacga ccttcctgac ggtgatacag caaaatacca cggccttcct cggcaatttg
    28561 cgtcaatgcc gcttcgagct ggaagccaca atcgcagcgc aagctgaaca gggcgtcacc
    28621 ggtcagacat tcggaatgga cgcgcgcaag taccggggta tgcccggaaa tatcgccata
    28681 gactagcgcg acatgatcgt gtccggttgc cagttcttca aatcccacca tcaggaaatc
    28741 gccccatggg gttggcagtt tggcttctgc cacacgttta agctgcatga aattctccag
    28801 ataatgctgg ttctgtattg gcttattttg ccataacgag aaggggttca cctaatcacg
    28861 acgcgtcgat cgttcacgga atggcacaaa tctgtcaatt tttcttggaa ctggcatttt
    28921 cagttatgat tgtgggactt atcaaaaagg agaggccatg cgttcgattg cccgacgtac
    28981 cgcagtggga gctgcactat tgtttgtcat gccagtagcc gtatggattt ctggctggcg
    29041 ttggcaacct ggagaacaaa gttggcgact aaaagcggct ttttgggtta ctgaaactgt
    29101 cacccagccc tggggcgtca ttacacattt gattttgttc ggctggtttc tctggtgtct
    29161 gcgttttcgc attaaggctg cctttgtatt attttccatt ctggcggcgg caatccttgt
    29221 tggacaaggc gttaaatcct ggatcaaaga caaagtacag gaaccacgac cttttgttat
    29281 ctggttggaa aaaacacatc atattccggt tgatgagttc tacactttaa agcgagcaga
    29341 acgcggaaat ctagtaaaag aacagttggc tgaagagaaa aatattccac aatatttgcg
    29401 ttcacactgg cagaaagaga cggggtttgc ctttccttcc ggtcacacga tgtttgctgc
    29461 cagttgggca ctgctggccg ttggtttgct gtggccgcgt cggcgaacgt taaccattgc
    29521 tatcttgttg gtctgggcaa cgggagtcat gggaagccgc ctgctactcg ggatgcattg
    29581 gccacgcgat ctggtggtag ctacgttgat ttcgtgggcg ctggtggcgg tggcaacgtg
    29641 gcttgcgcaa cgaatttgtg ggccattaac accacctgcg gaagaaaatc gcgaaattgc
    29701 acaacgagaa caagaaagtt aacgctggtt gattttccga atttaggcct taaatcatca
    29761 acaatgcgcg tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa agggctattg
    29821 cggtaagttg accataattt attcgctcta accacataac gggaagtaat gtgaaatatt
    29881 tactcatttt cttactggtg ttagcgatct tcgtgatttc ggtcacgttg ggtgcgcaga
    29941 acgatcaaca ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt atttccacat
    30001 tgctggcggt attgtttgct gcgggggttg ctatcggttg gttgatttgt ggcctgttct
    30061 ggctgcgagt tcgtgtttcc ctggcgcgcg ctgaacgtaa aataaagcga ctggaaaacc
    30121 agctttcacc cgcgactgac gtggctgtag tgccgcactc gtcagcggcg aaggaataac
    30181 tttctatgct ggagttgttg tttctgcttt tgcctgtagc cgctgcctat ggctggtata
    30241 tgggccgcag aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg tcgcgtgatt
    30301 acgtagcggg ggttaacttc ctgcttagta atcaacagga taaagcggta gacctgtttc
    30361 tcgatatgct taaagaggat acaggcaccg ttgaagccca ccttacgctc ggaaacctgt
    30421 tccgttcgcg tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta atggaaagcg
    30481 cctcgctgac ctatgaacag cgtctgttgg cgattcaaca actggggcgt gattacatgg
    30541 ccgccgggtt atacgaccgc gcggaagaca tgttcaatca gctgaccgat gaaactgact
    30601 tccgcattgg cgcgctgcaa cagttgctac aaatctacca ggctaccagt gagtggcaga
    30661 aagcaattga tgttgccgaa cgcctggtga agctgggtaa agataaacag cgcgtcgaaa
    30721 ttgcccattt ctactgtgag ttagccctgc agcatatggc cagcgacgat ctcgatcgtg
    30781 ccatgacctt gctaaaaaaa ggggcggcgg cagataaaaa cagcgcccgc gtatccatca
    30841 tgatgggacg cgtgtttatg gcgaaaggag aatacgccaa agccgtcgaa agtttgcaac
    30901 gcgtcatatc ccaggacaga gaactggtca gcgaaacgct ggaaatgttg caaacctgct
    30961 atcagcagtt gggtaaaact gccgaatggg cagaattcct gcaacgcgcg gtggaagaga
    31021 acaccggtgc cgatgctgaa ttgatgcttg ctgatatcat cgaagcgcgc gacggtagtg
    31081 aggctgcaca ggtctatatt acacgccagc ttcagcgtca tccgaccatg cgtgtattcc
    31141 ataagctaat ggattaccac ttaaatgaag cggaagaagg gcgcgccaaa gagagtctga
    31201 tggtgctgcg tgacatggtt ggcgagaagg tgcgtagtaa gcctcgttat agctgccaga
    31261 aatgtggttt taccgcatac accctctact ggcattgtcc gtcttgtcgt gcctggtcaa
    31321 ctattaaacc gattcgcggt cttgatggcc tgtaattttt aaaaaaatcc gactttagtt
    31381 acaacatact agttattgat gtcccattgt gctccggcaa cgacggagca gaaaaagcct
    31441 gctaggggaa caatcgcaac tgttaatttt ttatttccac gggtagaatg ctcgccgttt
    31501 acctgtttcg cgccacttcc ggtgcccatc atcaagaagg tctggtcatg acgttaactg
    31561 cttcatcttc ttcccgcgct gttacgaatt ctcctgtggt tgttgccctt gattatcata
    31621 attgtgatga cgcgctgtcc tttgtcgaca agatcgaccc acgcgattgt cgtctgaagg
    31681 tcggtaaaga gatgtttaca ttgtttgggc cacagtttgt gcgcgaactt caacagcgtg
    31741 gttttgatat ctttcttgac ctgaaattcc acgatatccc caacactgca gcgcacgctg
    31801 tcgctgctgc agctgactta ggcgtgtgga tggtgaatgt tcatgcctcc ggtggggcgc
    31861 gtatgatgac cgcagcgcgt gaggcactgg ttccgtttgg caaagatgca ccgcttttga
    31921 ttgctgtgac agtgttgacc agcatggaag ccagcgacct ggccgatctt ggcgtgacac
    31981 tgtcacctgc agattatgca gaacgtctgg cagcactgac gcaaaaatgt ggccttgatg
    32041 gtgtggtgtg ttctgctcag gaagctgtgc gctttaaaca ggtattcggt caggagttca
    32101 aactggttac accgggcatc cgtccgcagg ggagtgaagc tggtgaccag cgccgcatta
    32161 tgacgccaga acaggcgttg gcggctggtg ttgattatat ggtgattggt cgcccggtaa
    32221 cgcaatcggt agatccagcg cagacactga aagcgatcaa cgcctcttta cagcggagtg
    32281 catgatgagt gattccaaca gccgtctggt ctactcaacg gaaaccgggc gtattgatga
    32341 gcccaaagcg gccccagtac gccctaaagg cgatggagcg gtgcgtattc agcgtcagac
    32401 cagtggacgt aaaggtaagg gcgtttgcct gattaccggt atcgatctcg atgatgccga
    32461 actgacaaaa cttgcagcgg aactgaagaa aaaatgcggc tgcggcggag cagttaaaga
    32521 tggagttatt gaaatccagg gcgataagcg tgatttatta aaatcactac ttgaagcgaa
    32581 agggatgaag gtaaaactcg caggcggtta acataaaaag ccacggatat atccgtggct
    32641 ttcgaatatt ttactgtgtg tattattcag ttatttcgta ctgaacagaa
//
LOCUS       DADMFL010000038        32311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.038, whole genome shotgun sequence.
ACCESSION   DADMFL010000038 DADMFL010000000
VERSION     DADMFL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.038"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNZ27_001742"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNZ27_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY8664107.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JNZ27_001743"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JNZ27_001743"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY8664108.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JNZ27_001744"
     CDS             complement(3736..4092)
                     /locus_tag="JNZ27_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8664109.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JNZ27_001745"
     CDS             complement(4096..4512)
                     /locus_tag="JNZ27_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8664110.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="JNZ27_001746"
                     /pseudo
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="JNZ27_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
     gene            5699..6223
                     /locus_tag="JNZ27_001747"
     CDS             5699..6223
                     /locus_tag="JNZ27_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664111.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICTLCCFAE
                     EVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNKAKHILE
                     DIEIFYRNKPDTYY"
     gene            complement(6446..7639)
                     /gene="tyrB"
                     /locus_tag="JNZ27_001748"
     CDS             complement(6446..7639)
                     /gene="tyrB"
                     /locus_tag="JNZ27_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY8664112.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7892..8971)
                     /gene="alr"
                     /locus_tag="JNZ27_001749"
     CDS             complement(7892..8971)
                     /gene="alr"
                     /locus_tag="JNZ27_001749"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY8664113.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9024..10439)
                     /locus_tag="JNZ27_001750"
     CDS             complement(9024..10439)
                     /locus_tag="JNZ27_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY8664114.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10522..11505
                     /locus_tag="JNZ27_001751"
     CDS             10522..11505
                     /locus_tag="JNZ27_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY8664115.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVS
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11671..11913)
                     /gene="pspG"
                     /locus_tag="JNZ27_001752"
     CDS             complement(11671..11913)
                     /gene="pspG"
                     /locus_tag="JNZ27_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY8664116.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12047..13084)
                     /gene="dusA"
                     /locus_tag="JNZ27_001753"
     CDS             complement(12047..13084)
                     /gene="dusA"
                     /locus_tag="JNZ27_001753"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY8664117.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13447..14154)
                     /locus_tag="JNZ27_001754"
     CDS             complement(13447..14154)
                     /locus_tag="JNZ27_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY8664118.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDSTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14178..14432)
                     /locus_tag="JNZ27_001755"
     CDS             complement(14178..14432)
                     /locus_tag="JNZ27_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664119.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14750..15265
                     /gene="zur"
                     /locus_tag="JNZ27_001756"
     CDS             14750..15265
                     /gene="zur"
                     /locus_tag="JNZ27_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY8664120.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15307..15516)
                     /locus_tag="JNZ27_001757"
     CDS             complement(15307..15516)
                     /locus_tag="JNZ27_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY8664121.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15632..17011)
                     /gene="dinF"
                     /locus_tag="JNZ27_001758"
     CDS             complement(15632..17011)
                     /gene="dinF"
                     /locus_tag="JNZ27_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY8664122.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17030..17638)
                     /gene="lexA"
                     /locus_tag="JNZ27_001759"
     CDS             complement(17030..17638)
                     /gene="lexA"
                     /locus_tag="JNZ27_001759"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY8664123.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17748..18116)
                     /locus_tag="JNZ27_001760"
     CDS             complement(17748..18116)
                     /locus_tag="JNZ27_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY8664124.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18287..20710
                     /gene="plsB"
                     /locus_tag="JNZ27_001761"
     CDS             18287..20710
                     /gene="plsB"
                     /locus_tag="JNZ27_001761"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY8664125.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20865..21737)
                     /gene="ubiA"
                     /locus_tag="JNZ27_001762"
     CDS             complement(20865..21737)
                     /gene="ubiA"
                     /locus_tag="JNZ27_001762"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY8664126.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVRRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21750..22247)
                     /gene="ubiC"
                     /locus_tag="JNZ27_001763"
     CDS             complement(21750..22247)
                     /gene="ubiC"
                     /locus_tag="JNZ27_001763"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY8664127.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22470..24050)
                     /locus_tag="JNZ27_001764"
                     /pseudo
     CDS             complement(22470..24050)
                     /locus_tag="JNZ27_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24279..25199)
                     /gene="malM"
                     /locus_tag="JNZ27_001765"
     CDS             complement(24279..25199)
                     /gene="malM"
                     /locus_tag="JNZ27_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY8664128.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25351..26691)
                     /gene="lamB"
                     /locus_tag="JNZ27_001766"
     CDS             complement(25351..26691)
                     /gene="lamB"
                     /locus_tag="JNZ27_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY8664129.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26763..27878)
                     /gene="malK"
                     /locus_tag="JNZ27_001767"
     CDS             complement(26763..27878)
                     /gene="malK"
                     /locus_tag="JNZ27_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY8664130.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLEAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     ALVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28243..29433
                     /gene="malE"
                     /locus_tag="JNZ27_001768"
     CDS             28243..29433
                     /gene="malE"
                     /locus_tag="JNZ27_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY8664131.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29587..31131
                     /gene="malF"
                     /locus_tag="JNZ27_001769"
     CDS             29587..31131
                     /gene="malF"
                     /locus_tag="JNZ27_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY8664132.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31146..32036
                     /gene="malG"
                     /locus_tag="JNZ27_001770"
     CDS             31146..32036
                     /gene="malG"
                     /locus_tag="JNZ27_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY8664133.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8226 a         8287 c         7799 g         7999 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatctta gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttatac aaagcggaaa aaaagagttc tctttaacat tagaatttaa
     6121 cgatactata attgaatctc agaaagtcac aggcaataag gcaaagcata ttcttgagga
     6181 tatagaaata ttctatcgta acaaacccga tacttattat taaatttaat aatggttaga
     6241 tgtgcagaaa ttattactcc acacgaaaaa atagggaaag gaattccaga taattgggat
     6301 aaacttaaca ataaaagaac tgacaacgtt ttattatctc tcacgtagaa caacggcatc
     6361 aaaaaaatca tctacaaaga aatagtgaaa gcgacgacag tttaatttca ctgcaggctg
     6421 ggtaactcca gcctgctttc ctgcattaca tcaccgcagc aaacgccttt gccacacgtt
     6481 gtacatttgc cgtatttaac ccggcgacac acatgcgacc gctggcgatg agatagacac
     6541 caaattcttc acgtagtcgg tcaacctgag cggcacttaa accggtataa ctgaacatgc
     6601 cgcgctgatt aagcagataa tcgaaattgc gttctggcat ctctgtgctt aataccttca
     6661 ccaattcctg acgcattgcc agaatgcgag tacgcatctc ttctacttcc gccagccagc
     6721 tggctttcaa tgcctcgtca ttcagcactg cagccaccac ctgcgcacca aaattcggcg
     6781 ggctggagta gttgcggcga actgttgctt tcaattgccc cagtacgcgg ccagcggctt
     6841 cggcatcttc acacagaaca gaaagtccgc cgacgcgctc gccgtaaagg gagaaaattt
     6901 tcgagaacga attgctcacc agagcgggta atccagcgct ggcaatggcg cgaatagcgt
     6961 aggcatcctc ttccataccg gcactaaatc cttgataggc aatatcgagg aatgggataa
     7021 gctcgcgggc tttgagaatt tcaatcaccg catcccactg atcattagtg agatcggcac
     7081 ccgttgggtt gtggcaacat ggatgcagca acacaatact gcgggcaggt aatgttttca
     7141 gcgtcgccaa caggtcatta aagcgcacgc tgttagtcac ttcgtcatac caggggtaag
     7201 tactcacttc gaatccagcc ccggcgaata ttgctacgtg gttttcccaa gtaggatcgc
     7261 tgacccagac gcctgattcc gggaagtagc gtttcaggaa atccgcgccc actttcaatg
     7321 cccctgagcc gccaagggtt tgaatggttg ctacgcgctg ttgttgcagt accggatggt
     7381 ccgcaccaaa cagcagcggc gcaatggcat ggcgatagct gttaagccct tccatcggta
     7441 aataaagcga agcgccatgg ggctgcgcat tcaggcgcgc ttccgcctcc gccacggctt
     7501 tcaattgtgg aataattccg tcttcgttgt agtacagacc gatacttaaa ttcactttgt
     7561 cgctgcgagg gtcttcttta aaacgctcca taagcgtaag aatcgggtcg ccagcgtagg
     7621 cgtcaacttt ttgaaacacg cgatggttct ccaggtttac gggcaggtgg ttaaaacaca
     7681 ataaaccgga agaaggcgaa gatcgagtgg atgttcagga gcgaacggca attagcaaca
     7741 gagtgagact aatgataaac gtacatccgc cagaggcacg acctttataa gagcatgaaa
     7801 gaacatcaac ttattgaatt tttaggattt tattggccgg ataaggcatt cacgccgcat
     7861 ccggcacaga caatcaaata ttacagaacg attaatccac gtatttcatc gcgacccttg
     7921 aagtcaggcg cgtaataagt tcgtaagcgc ttactttcgt catttcagcg atacgttcta
     7981 cgggcaaacc ttcgccccat aaaatgaccg gatccccggc tttgtcctgc gcctgtggac
     8041 ctaagtctac gcagatcata tccatcgcca cgcgcccgac aatcggtact tcgcgaccgt
     8101 tcaccagcac tggcgtaccg gacggcgcgg cgcgcggata accatcgcca tagcccatcg
     8161 cgactacgcc aagacgggta tcacgttcgc ttacccaggt tccaccataa ccaacaggct
     8221 ctccggcttt atgctcacgc acggcaatca ggctggaggt tagtgacatc actggctgac
     8281 agccaaaatc ggcaccggtg gagcgatctt ccagcggcga gacgccataa agaatgatgc
     8341 ccgggcgcac ccagtcaaaa tgcgactgtg gccacagcag aatgccaccc gacgcggcaa
     8401 tggaacgttg accaggtttg ccttcgcaaa aggtattaaa gatagcgagt tgtttctcgg
     8461 ttgcgccaca ttttggttca tccgcgcgcg caaaatggct gacgatattc accggctgac
     8521 gaacgttttt gcactgggtc aggcgatgat aaaacgcctc agcctgttcc ggccttacgc
     8581 ccagacggtg cataccggta tcgagtttca tccagacggt aaccggctcg tccaagctag
     8641 cctcttccag cgcagccagc tgttcttcgt tatgcacggc ggtatgaaaa tgttgcgcag
     8701 aaatcgtcgg cagatctctg gcatcaaaaa agccttcgag taacagtaca ggtttggtga
     8761 ttccccccgc acgcagtcgc agagcttctt cgagacgggc tacgccaaag gcgtcagcat
     8821 cggggagcgt tcgcgcggtc tcaagaagac cgtgaccata agcgttcgct ttcaccaccg
     8881 caaccatttt actggcaggt gccagttcac gaagacgttg caggttgtgt cgcagagcgc
     8941 ggcggttaat cacaacagtt gccgcttgca tttgtgttcc ttgataattg tttgctttaa
     9001 ttacctaatt cataaaataa ttattattcg tcgtcgtact gcggccccgc atagttgtcg
     9061 aagcgcgacc attgaccgtt aaaggtcagg cgtaccgtcc cgattgggcc gttacgttgt
     9121 ttaccgataa taatttccgc gatgcctttt aaatcactgt tttcgtgata cacctcatca
     9181 cgatagataa acatgatcaa gtccgcatcc tgctcgatag agccagattc acgcaggtcg
     9241 gagttgaccg gacgtttgtc ggcacgttgt tccagagaac ggttcaactg ggacagcgcc
     9301 accaccggca cgttcagttc tttcgccagt gctttcagcg agcgggagat ttctgcaatt
     9361 tccagcgtac ggttatcgga aagcgccggt acgcgcatca gttgcaggta gtcgatcatg
     9421 ataagcccga tgccgccgtg ttcacgggca atacggcgtg cgcgggaacg cacttccgtt
     9481 ggcgtcaggc cggaagagtc atcgatatag atattgcgtt tttcgagcaa aatacccatg
     9541 gtgccggaaa tgcgcgccca gtcttcatca tcgagctgcc cggtacggat tttagtctgg
     9601 tcaacgcgcg acagcgacgc cagagaacgc atcatgatct gttctgatgg catctccagc
     9661 gagaagataa gtactggttt atcctgcaac attgccgcgt tttcgacgag gttcatcgca
     9721 aatgttgttt tacccatcga cggacgcgcg gcgacgatga tcaaatccga cggctgcaag
     9781 ccagcggttt ttttgttgag atcgtcataa ccggtgttta ccccggtaac gccatcgtgt
     9841 ggctgctgaa acaactgctc aatacgcgcc acggttgcgt cgagcacatc ggcgatgttc
     9901 ttcggccctt cgtctttgtt tgcacgactt tcggcaattt taaagacgcg ggattcagca
     9961 aggtccagca gatcttcgct ggtacgcccc tgcggatcaa aaccggcttc agcaatctca
    10021 ttcgcaaccg agatcatctc acggacaacg gcacgttcac gcacgatgtc cgcataagca
    10081 ctgatgttcg ccgcacttgg cgtatttttt gacagctctg ccagataagc aaaaccaccg
    10141 acgctatcga gttgcccctg gcgttccaac gattccgcaa gggtaatcag atcgatagga
    10201 ctaccgcttt cctgcaaacg cgccatttca gtaaagatat gacggtgtgg gcgggtgtaa
    10261 aaatcgtctg ctaccacacg ctcggctaca tcatcccagc gttcgttatc cagcattaaa
    10321 ccgcccaaca ccgactgctc cgcttcgatc gagtgcggag gcactttcag cccggcaact
    10381 tgtggatcgc gttcgcgggg ttcagcctgc tgtttgttga agggtttatt tcctgccata
    10441 gtgaatggag ttaccgaaat aaagaatggg tcgaaacttt accatatgaa gcagaccctg
    10501 acgatacgtt ctggaggaca catggcaaca cgaattgaat ttcacaagca cggtggcccg
    10561 gaagtacttc aagccgtaga gttcactcct gccgatccgg cggagaatga aatccaggtc
    10621 gaaaataaag ccatcggcat caattttatc gacacgtata tccgcagcgg cctttacccg
    10681 ccgccatcgc tacccagcgg attaggtacc gaagcggcag gcatcgtgag taaagtcggc
    10741 agtggtgtaa agcatattaa ggctggcgat catgtagtct atgcgcagtc ggcgttaggc
    10801 gcttacagct ctgtgcataa cattaatgcg gataaagcgg cgattctgcc tgcggcaatt
    10861 tcttttgagc aagctgcggc atccttcctg aaaggcttaa cggtttatta tctgctgcgc
    10921 aaaacctatg aaattaaacc cgatgaacag ttcctgttcc acgcagcggc tggcggcgtt
    10981 ggcttaattg cctgccagtg ggcaaaagcc ctgggcgcga aactcatcgg caccgtagga
    11041 accgcgcaaa aagcgcagag cgcgctaaaa gccggcgcgt ggcaggttat taactatcgt
    11101 gaagagaatc tggtcgagcg attgaaagag atcaccggcg gtaagaaagt gcgcgtagtg
    11161 tacgattccg tgagcagaga cacctgggaa cggtcgctgg attgcctgca acgccgcggc
    11221 ttaatggtca gttttggcaa ctcatcaggt gcggttaccg gtgtgaactt aggcattctc
    11281 aatcaaaaag gctcgttgta tgtgacacgc ccttccctgc aaggctatat caccacgcgg
    11341 gaggaattaa ccgaggccag taatgaactg ttctctttga ttgccagcgg tgtgattaag
    11401 gtcgatgtcg ccgagcagca gaaatatccg ctgaaggatg cgcagcgtgc gcatgagatt
    11461 ctggaaagcc gggcgacgca aggttccagc ctgctgattc cataaaagaa atagggcttc
    11521 cacctgggaa gccctttctt tttatagttc ggctgtatgt agggtacagc acgatgaatc
    11581 tgttagaggc gcaataatga cagatttgat tatcaattcc tattttgttc taaggataaa
    11641 actttaggtt gtgatcatcc gcacaatccc ttagtaacgc cagcggtcat aacgctgata
    11701 tttcggcatt tttggtgctt taatcgcctt aataacccac accaccgcaa tcgccagtag
    11761 taaccacggc agcaacttaa tcatcaatgc cagcataccg ccgaggaaca taatggccgt
    11821 cgccacaacc agcgcggcga taatgcccag caacgaaacg ccggtgacca tcagcatgac
    11881 aaaaaagcca atcacaaaaa gtagttccag catgatgctc tcccaaatat gaaatctctt
    11941 gctggcatta caagaatcat gccaaaaata atctattgat ttaacagcaa aacgccccgc
    12001 gacggtgcgc agggcgtggt gaatttgact actttttggt gaaaagttaa cgcttatccg
    12061 ccaccagttt gagcgcgtgt tccagcacgt taatgtcagc acccgcttta tgggcatttt
    12121 cacttaaata acgccgccac tgccgcgcgc caggaatacc ctggaacaaa cccagcatat
    12181 gccggataat atggccgaga tacgtcccct ggctgagttc atgctcaatg tacggataca
    12241 tggcacgcac tactgccacc ggatcggcat cggtatccga ggaaccaaag atctcccggt
    12301 ctaccgccgc cagaataccc ggattctgat acgcctcgcg cccgaccatc acgccatcca
    12361 tatgttgcaa atgcgcttta gcttcttcca gcgacttgat accaccgtta atcgacattg
    12421 tcagatgcgg gaagtcacgc ttcagttgat acacacgcgg ataatcgagc ggcgggattt
    12481 cacggttttc tttcgggctt aaccccgaaa gccaggcttt acgtgcgtgg atgatgaaca
    12541 tctcacactc gcctttgccg gaaacggtat tgatgaaatc gcagagaaat tcatagctgt
    12601 cctggtcgtc gatgccaata cgcgttttca ccgtcaccgg aatcgacacc acatcgcgca
    12661 tcgctttcac gcagtcggca accagctgcg cattacccat cagacacgca ccaaacatgc
    12721 cgttctgcac ccggtcagac gggcagccga cattcaggtt gatctcatca tatccgcgcg
    12781 cttctgccag ctttgcacac tgcgccagcg ccgccggatc gctaccgccc agttgcaacg
    12841 ctaccggatg ttcttcttca ctgtacgcca gataatcacc tttaccgtga ataatcgccc
    12901 ctgtggtcac catttcggta taaagcaacg tattgcggga aagcagacgc aagaaatagc
    12961 ggcaatgtct gtccgtccag tcgagcattg gtgcaacact aaaccgacca ctccagtgaa
    13021 cgtcagtttt ttcaggcatt gcgctggttt ggttgatttt ttgcatttca gaattaccgt
    13081 gcattttcaa atgtagagat tattttatcg atatatcatg gggttatgtt attcagcttc
    13141 actgttcagg aggctcaata gcggggtact ataccataac aacaggaagc gcctgtctca
    13201 ttgcaaaaga aaattgagat cctctcaagg catgaagctc tcacgaagtg atggaaataa
    13261 tcttattagc cgttaacttt gttaaggcca atgataaaca atccaggttc gacgataaat
    13321 aaaaaatcac acattaaact ctggtgatat atctccctgc taatagcatt gatagagaaa
    13381 aaagaaccca ataaatattg ggtcctttta tataatgcct tccatactat cgaagaactt
    13441 cacatattat ttctccgatt taaccccgaa caagtcataa attaatttag aagtgtctgt
    13501 aagtatttta atctcttcct ttgaggttga gtcaaacgac ttcgcgaagc taattaattg
    13561 tggtgcagca tctcgcattt tgcttaaaat atcaggatcg agcgttcctt cattcaccag
    13621 atgtgacatc ttatccagat agccatcaaa attcatttct cccccatctg ccagacgctt
    13681 catctctccc aggtacttct tcatatcacg ttgatataat ttttcaaact gagctttcag
    13741 gtagctctca ttattttcat taatatacac tgtttctgat aaatctgcgt aggcactaga
    13801 atatgaaagt gaaattaaca gcgaagcaaa taattttaag ctgttgtcat catgttcctg
    13861 gcaggcatta acaaacgtta aaaaaccaga acctattgct tgaaaatgta tattcgctaa
    13921 aggttcatta tccttagatt cctcataaaa tagctgaacc gtggagggaa ggccattctt
    13981 agtttctaca tcaaaagtac attgttcaat gggcattgca tctttattat ccccaggatt
    14041 acatatacta ttaatcgcga aatgaaaaat agcccggtca acaactgatg caataaagtc
    14101 acgatcgttt aaatcctcat cagtgcaatc gtcagtatac ttattgaccc acattttgag
    14161 atcatttatt tgctcaatta aaaacacatt tttttacgtg catgccataa attatgcgtg
    14221 attcgttgaa taaacaaatc tatttttgca ataattatat cccgatgacg cagcgctgca
    14281 taatcaatag atttaccata tatattattt aatttacctt tgattgaacc tctattatcc
    14341 aatttatctt tggttgcatc tttattttcg aattgatttg aattgagtgt tttattaaag
    14401 tatccagttg caccgggtat tatcttcaac atgattcatc tacctttttt gtcgtaccgt
    14461 gtcctcctta taattctttt tccatctggt cactatcaaa aatcgctcat aatttctgaa
    14521 ctacaggtat gcgtttaaac ccagttaaat aaatagcgag ttaacttgaa gcaatttatt
    14581 taaaaatgaa ttattaatta ttatcgtgtt gataatggta cgacaaggca acacaacacc
    14641 ttacctacaa aacagaaaat caacattacg gcaacaataa gggttctcgg tgtttttcct
    14701 ggaacatggt aaagtaagga cattcttaac ccccactttg aggtgcccga tggaaaagac
    14761 cacaacgcag gagttattag cgcaggctga aaaaatctgc gcgcagcgta atgtgcgcct
    14821 gaccccacag cgcctggaag tgttgcgcct gatgagtctg caggatggcg ctatcagcgc
    14881 ttatgatctg cttgatttac tgcgcgaagc tgaaccgcaa gccaagccgc caacggttta
    14941 tcgcgcgctg gattttctgc ttgagcaagg ttttgtgcat aaggtggaat ccaccaacag
    15001 ttatgtgctc tgtcatctgt tcgaccagcc cacgcatacg tcagccatgt ttatttgcga
    15061 tcgctgcggc gcggtgaaag aagagtgtgc agaaggcgtg gaagacatta tgcatacgct
    15121 ggcggcaaaa atggggtttg ccctgcggca taatgtgatt gaagcacatg ggctctgtgc
    15181 ggcatgcgtg gaagtggaag cgtgtcgtca tcctgaacag tgccagcatg atcactctgt
    15241 gcaggtgaaa aagaaaccgc gttaagaggg cgtacatcct tgtacacgtc gggcaggagg
    15301 gattaattac cagcgatatt cattgcgggt ttcccaatcc acgacctctt tttctgcctg
    15361 atctttctga taaccataac gttcctggat tttaccgacc agttgatcac gtttaccttc
    15421 aatgatcgtc atatcatcat cggtcagttt gccccattgc tctttcactt tacctttaaa
    15481 ctgtttccag ttaccgccgg cttcatcttt attcataatc aagacctcat cgttaggttg
    15541 tgaatgagag tacgttcact tttcttctga acgtgagatt aagtgtagtc agcaatttgg
    15601 cttaggataa tttattcaga atttttaacc gtcacgttgc ggcaaaccag gtaccgttgc
    15661 gccagtgacg ccgccagata gccgccagag aaagcccgcg caacgccaga aagacggtta
    15721 atgccagcca caaaccatga ttacccagcc acggcagcgt aaggagcgtc agcgcaaaac
    15781 ctgcggcggc caccgccata ctgttacgca tttcggcggc acgcgttgcg cctataaaca
    15841 tgccgtccag cagataacac cagacgccaa ccaacggcaa aatcacctgc cagataagat
    15901 agcggtcagc cagctgctga atctgggtta acgacgtcag caacgcaatg atgtgttccc
    15961 ctgccagcaa ataaaccacc gaaaacagta acgctacaat ccccgactgg cggcacgctg
    16021 cccgccatac atccagcaac tggctaccgt cgcgcgcacc gtatgcctga ccggagtgcg
    16081 cttcaaccgc gtaggcaaaa ccatcaagcg cataggcggt aaaggtgagt agcgtcatca
    16141 gaaccgcgtt aacagcgata atgtcactcc ccagtcgcgc accaagtacg gtgatcgcgc
    16201 cgaaacagag ttgcaacaac agcgaacgca gcatgatatc gcggttaagc gccagcaagc
    16261 gacggaagtt tcctcgccag gcagttttca gcatttcgcc ggaaattccg cgtagtttga
    16321 ggattttacg caccattagc agaccaatca gcaatgttgc atattccgca ataaccgtcg
    16381 ccagcgccgc gccctgcacg ttcatatgca gccccatcac cagccagaca tccagcacaa
    16441 tgttgaggat attgccgacc actaacaaaa ttactggcgc acgggcatat tgcacgccga
    16501 gtaaccaacc gagtaatacc agattcgcca gcgacgccgg agcgcttaac cagcggattt
    16561 caagaaagcg ccgcgcctgt tccaggaccg cttcactacc gccaacaata tgcagcgcca
    16621 gatcgataat cggcgtacgc aacagcgcaa ttaacgcccc ggcccccaac gccaacagca
    16681 acggttgcac cagcgcacgg gctaatgcct gaggattttt ggcaccataa gcctgcgcag
    16741 tcagcccggt ggtgctcatg cgtaaaaaca gcaacagcat aaagagaaag ctggtcgccg
    16801 ttgcaccaac cgccacgccg cccaaataaa ccggactatc aagatgacca attaccgccg
    16861 tatcgaccag tcccagcaac ggaacggtga tattggagaa aatcatgggt aaggcgagat
    16921 gccagagtgc tttatcagat gaagtgagga atgccatgca gacaagcctg atgaagagag
    16981 atgaaaaaca aaccgcgacg ccaggcggca tcgcggtctc agagatatgt tacagccagt
    17041 cgccgttgcg aataacccca accgccagcc cttcaatggt gaagctctgc tgacgaaggt
    17101 caacgacaat tggtttaaac tcgctatttt ctggcaacag ttcgacttta ttgccctgtt
    17161 ttttcaggcg cttaacggta acttcgtcat caatacgtgc gacaacgacc tgaccgttac
    17221 gtacatcctg agttttatgc actgccagca agtcgccatc cataatgccg atatctttca
    17281 tcgacatccc gctgacgcgc agcaggaaat cagcattcgg cttgaacaag gaaggatcga
    17341 cctgataatg accttcaata tgctgttgcg ccagaagcgg ttcaccggca gccacacgac
    17401 ctaccagcgg caacccttct tcctcttcct gcaacagacg aatcccgcgt gatgcgccgg
    17461 aaacaatttc aataacgcct ttgcgtgcca gcgccttcag atgttcttca gccgcgtttg
    17521 gggaacggaa ccccaaacgc tgcgcgattt ccgcacgcgt cggcggcata cctgtctggc
    17581 tgatgtgatc acggatgaga tcaaacacct cttgttgcct ggccgttaac gctttcattc
    17641 cgccccctgg gtgtatatac agttatactg tgagtatata cagcaaaagg cgattttgga
    17701 accataaact gcacaataaa ccagagattt atcgaaatct ggaagggtta tccaaaatgc
    17761 gaccataaca ggatgcacca ggtaatcacg gcgacgataa tggcaatcag caccgcggcg
    17821 gatcccatat cttttgcgcg tccggaaagc tcatggtatt cagagccaat tcggtcaacc
    17881 actgcttcga tggcgctatt gaggatttcc acaatcatca ccagcatcac ggagctgata
    17941 agcagcacgc gggtaattgc gtccacatcc agccagcagg cgatgaccac cgccaacaat
    18001 accgctacgc cttcctgacg gaatgccgct tcgttgatcc atgcagcgcg taaacctttc
    18061 caggaatagc cagcagcttt gataattcgg gtgaatccag tggtattatt ggccattgaa
    18121 agaacctttt tacattatga gcgtcaatat cagtgtaccg ttaaatccga tgattcgggt
    18181 ttgcgtaacg ccgcgcgaaa catgagcgga taccacagaa tttcccatga ctttctgcta
    18241 tccttgccgc gcatttgcat tattaaccag aggctttaca tcgtttatgt ccggctggcc
    18301 acgaatttac tacaaattac tgaatttacc attaagcatc ctggtaaaaa gcaagtctat
    18361 tccggcagat cctgccccgg aactggggct ggatacctct cgtccaatta tgtacgtttt
    18421 accgtacaac tcgaaagcag atttgctgac gttgcgcgcc cagtgtctgg cacatgattt
    18481 gcctgacccg ttagagccgc tggaaatcga cggcacgcta ctgccgcgct atgtgttcat
    18541 tcacggcggg ccgcgtgtat tcacctatta cacgccgaaa gaagagtcta ttaagctttt
    18601 ccacgactat ctcgatttgc accgtagcaa cccaaatctg gatgtgcaga tggtgccagt
    18661 atcggtgatg tttggtcgcg cgccggggcg tgaaaaaggc gaagtgaacc cgccgctgcg
    18721 tatgcttaac ggcgtacaga aatttttcgc tgtactgtgg ctcggtcgcg acagttttgt
    18781 gcgtttctcg ccgtcagtgt cgctgcgccg tatggcggat gaacacggca cggataaaac
    18841 tatcgctcag aaactggcgc gcgtggcgcg tatgcacttt gcccgtcaac gtctggctgc
    18901 cgtaggccca cgtctgcctg ctcgtcagga tctgtttaat aagctgctcg cctcccgcgc
    18961 cattgccaaa gcggtagaag atgaagcgcg cagcaaaaaa atctcccatg aaaaagcgca
    19021 gcagaacgcg attgcgctga tggaagagat tgcggcgaat ttctcttacg agatgatccg
    19081 cctgactgac cgcattctgg gcttcacctg gaaccgattt tatcagggca tcaacgtcca
    19141 taacgccgag cgcgttcgcc agctggccca cgacggtcat gagctggtgt atgtgccttg
    19201 ccaccgcagt cacatggact acctgctact ttcttacgtg ctgtaccacc aggggctggt
    19261 gccgccgcat atcgccgccg ggatcaacct gaacttctgg ccggccgggc cgattttccg
    19321 ccgtctgggg gcgttcttta ttcgccgtac ttttaaaggc aataaacttt attccaccgt
    19381 tttccgtgag tatctcggcg aactgttcag ccgtggttat tccgtcgagt acttcgtgga
    19441 aggcggtcgt tcccgcacgg ggcgtttgct ggatccgaaa accggtacgc tgtcgatgac
    19501 cattcaggcg atgctgcgtg gcggcacgcg tccgattacg ctgattccga tctatatcgg
    19561 ttatgagcac gtcatggaag tgggtactta cgccaaagaa ctgcgcggcg cgacgaaaga
    19621 gaaagagagc ctgccgcaga tgctgcgcgg tttaagcaag ctgcgtaatc tcggtcaggg
    19681 ttacgtcaac ttcggtgaac caatgccgtt gatgacctac cttaaccagc acgtaccaga
    19741 ctggcgtgaa tctatcgatc ccatcgaagc ggtgcgtccg gcctggttaa cgccgacggt
    19801 caataatatt gctgccgacc tgatggtacg cattaacaac gcaggcgcgg caaacgccat
    19861 gaacctgtgc tgtactgcgc tactggcatc acgtcagcgc tcactcaccc gcgagcagtt
    19921 aaccgagcaa ctcaactgct acctggatct gatgcgcaac gtaccttact ccacggactc
    19981 taccgttcct tcagccagcg ccagcgagct tatcgatcac gcgctgcaaa tgaacaagtt
    20041 tgaagtcgag aaagacacta tcggcgacat catcattctg ccacgcgagc aagcggtgct
    20101 gatgacctac tatcgcaaca acattgcgca tatgttggtg ctgccttcgc tgatggcggc
    20161 aattgtcacc cagcatcgcc acatctcccg cgacgtattg atggagcacg tcaatgtgct
    20221 ttacccaatg ctgaaagcgg agctgttcct gcgctgggat cgcgacgagt tgccggacgt
    20281 tattgatgcg ctggcaaatg agatgcaacg tcaggggctg attaccctgc aagatgatga
    20341 gttgcatatc aacccggcgc attctcgcac cctacagctg ctggccgcag gcgcgcgcga
    20401 aacgctgcaa cgttatgcca tcaccttctg gttgttgagt gccaacccgt cgatcaaccg
    20461 cggtacgctg gagaaagaga gccgcaccgt tgcgcaacgt ctctccgtgc tgcacggcat
    20521 caacgcgccg gagttcttcg acaaggcggt gttcagttct ctggtgctga cgctgcgtga
    20581 tgaagggtat atcagcgata gcggcgatgc cgaaccagca gaaacgatga aggtttatca
    20641 gttgctggcg gagttgatta catcagacgt gcgtttgacg attgagagtg cgacgcaggg
    20701 cgaagggtaa tcagagagaa ttgccggatg cggcgaaaac gccttatccg gcctaccatg
    20761 acctgcaaat tcaataaatt gcgattcacc gggtaggcct gagaagcgca gcgcatcagg
    20821 caacccagaa gaaagccgga tgatcatccg gcttttttac atcatcagaa atgccagtaa
    20881 ctcattgcca gtcctaaaaa cagtaccaga ccaacatagt tattattcat aaatgcttta
    20941 aagcaggctt cacgctcgcg gttggcaatc agtttttgtt gataaacaaa cagcgcgcca
    21001 gccaccacaa ttgaccagta atatccccag cctaagccat ttaactcacc gatgatcgcc
    21061 atcagtgcca gtacgccaat ctgcaaaata ccaataatca atttatcgta ttggccgaac
    21121 aggattgccg tggatttaat gccaatcttc acatcatcat cgcggtcaac catcgcatac
    21181 tgcgtgtcgt aagccaccgc ccagagaata ttggcgagga acattaacca gcaactcaat
    21241 ggcaccgact cactcacagc ggcaaaagcc attggaatcg accagccaaa cgccgcgccc
    21301 agcaccactt gcgggagatg ggtataccgc ttcataaacg ggtacaccca cgccaatgcc
    21361 agagcggcaa tcgacaacag aatggtcatc gtattcagcg tcagaaccag taaaaacgaa
    21421 atcagtacca gcactacaaa cagcgcgcgc gcctctttct ctgttaccgc gccgctggga
    21481 agtggtcggt tcgccgtgcg cctaacatga ccatcaaact tgcgatcagc ataatcattc
    21541 accacgcaac cagcagcgcg catcagccag acacccgcga caaacaccgc caggatccag
    21601 agctgaggaa cgcccggtgt cgccacccac aacgcccata atgttggcca gagcagcagt
    21661 aacgcgccaa ttggcttatc cgtacgcatt aagcgatgaa acgccagcag cttattctgc
    21721 gtcagactcc actccatatt tttttcctct tagtacaacg gtgacgccgg taaaaacagt
    21781 tctgttagca acagcggttt accgcttaat cgcaggcggg aacgtcgccc ccacagtccg
    21841 gcatcacggc ctatctcgat aaaatcccgg gttagtgtcg atgatgtgaa cagatagcgt
    21901 cctaacggcg ttttacccaa tttttgtaac gctagttccg gcccgcttaa tgttgataca
    21961 ggaacgacgg tacgaccggc aagccacggt tcaccatcgg cacataacaa aatttcacgt
    22021 aaccagtaac gagactcttt cggcagtagc ggcagttctt cggggatttc attctgctcg
    22081 acaaacccct cgcggatcat cgtcacactc accgtttttc cctgctgttc aaaacgtttt
    22141 gtcatggaat cctccagcaa cagccagtcg aacagttgcg gatccagggc agggatctct
    22201 ttaaaatagc gcagcgcacg cagttgcgtt aacgcggggt gtgacatgcc gaactctccg
    22261 ttacataacg taaaggcatt gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt
    22321 aagtgatcta atgcgcaaca aaaacgcaac aatcgcatga agtggtaaaa cgctgttgaa
    22381 ccttacgctt tgccgatgcc cgtgctggcg agggcaacca taggggcatc accgcccatg
    22441 atgctaggaa cggtgatgtt ttcctttact taaatacctt tattctttat acgagtagct
    22501 cgccaactcg cagtaaaaat tgggaaaagc gcatatttac catgagattt ggagctgaaa
    22561 agtgtttcct gagtaagatt aataggcaac atcttatgtc tggatttata gatacacgtg
    22621 gaattccaat tggtatcttt tgtaggattc aacatatcat gtaagcgctg ggagcttacc
    22681 accataacag gcccatcatc gacagaggag aacaggataa atatattatc gtttcgtgtt
    22741 aatttattag tgttaaaggt tcttaacttt tcttcagttt ctataaatat aggtgaaaca
    22801 tcatcactac gaatccactc tttataaaga taccaggcat ttgctttgat catatcatcc
    22861 gttgatcgta aggcctgatc gataagttgg ataaaaaaat tgttattttt atcaaccagc
    22921 cctttattca tattaaatct gttaataaaa tggatcatct gttgggtatc gaaattcacc
    22981 ttactgaatc gatgttgacg cataaaatta tcgaacttgt catcttcccc agaatgtata
    23041 taactacgta taattggatc ttttatataa ggacaatggc taaatgtttt cttcatattc
    23101 tcaataaagt ttttattaac aatattgaga atttttaatt gcatcaccag ttttactgcc
    23161 agaacggatt tcaatatcat tgcattatct ggcaatgtat caatcatttt tataatgtta
    23221 ttgatataac catcgagatc ttcattccca aatatatttg aataatcaat agttaattcc
    23281 aattgttctg taatgcaatc ttaaaaattc cctgtcgaca ttcgcacatg atcaaacata
    23341 acagctgtca aattcgattt aataaatgat atttctttgg cagacagaaa ctctgctctg
    23401 gtgaagtgcg aagcgtaaag ataacaatta tcgaagtttg aatttctaat ctgacattcg
    23461 cgaaaattac tatgaccaag ttgactctgc gagaagtcaa ctgtatcaag aatattttgg
    23521 ctaaaatcaa cacattcgcc actcgttttg agaaaaatca cattgtgtaa acgtcgacat
    23581 ttaaatatga cattatgaac tgttgcctta gtaaaattgc aattctcaat ttctttacat
    23641 tcgaaagtta catgttgtag atttgatgat aaaaaagaca ccttagaaat aacgtcacct
    23701 ttaaaatgac aatttgttaa atcaaaatca gacaggtcga tatacccctg cgcatcttta
    23761 tccatcagaa aattacaagc gcattctatt ttttcatttc tgtagaattt tacacttata
    23821 gtatcattac taattgagac ttcacgtaac atatttattg catgattttt ttcgttccgt
    23881 tgttttatat ttaaaaatat atcactgata taaataaagt tatctctgtt taaacctgtc
    23941 accccattga aattacgtag taaatcaaga tctactatat tatttccttg caccacatca
    24001 tgtttcatca aagagttatt gccgccagaa tttgtgatat gacctatcat aaaacctctg
    24061 taatacagaa ataaaataac ctggaaaagt agcctgagcg tccagacact accggcgaaa
    24121 ggaaaagaat ctctgataat gcattgaaat aatggacatt cttagtgaag aagaagtgaa
    24181 gtactataaa aaatcgctca tatcccagga aggaagcata aaaaaaggtg cgccaggaga
    24241 cgcaccagtt gttgcaaaat cagcactgtg gggcgtaatt accccttgcc ttttacactg
    24301 ctgataaagg tggaacgggc agatgtcgat cccaggcgtt cagcttcatc aagcagtttt
    24361 aacgccttat caacatcacc tttcgcgaca gcgtttttga tcgcggtatt aaaataactt
    24421 tccgtgtcgt tgagcatcgg ctcgcttttc ttcaccggca ccggagcagg tgcagccaca
    24481 gctggtgccg ccgtgttgcc taccgtaacc ggagctggag cggaagaacc aaatagtggt
    24541 cctaccaaca cgctggagct ggagttcgtt ttgactttca gtttcagtaa gccatcgctg
    24601 gtatgacgag caaccggatc gggaatatcc gggatcgagt taccgacgcc tttggcatag
    24661 gctttagccg ggtcgagcag ttgggtcgtc tgctggagat ctttttccat agtaaagacc
    24721 agaacataaa gtttttgctg ccccaacgcc ggtgtcaggc gcataacgcc ttccagccga
    24781 tctgcactca tcacgcctag ttcctggtag gtgaaataac tgctggggaa gaaggctgat
    24841 ggggtcatgt tctgatcaat aatcagcacg ttcggcgcaa aaacgctggt ttgtttgttc
    24901 acttcgctgg tcagcgtcag ggtcagttcg ccaatgtttg ccgggacgct gtacgcagca
    24961 accggaccac tgatgccggg aacgttcagt tgttggccgc cggtcgccag ttgggtggtc
    25021 tgggttttag attgatcgac cggtgtccag gtgagttgtt gcagcgcggc agatggaatg
    25081 gctggcgcgt cgctggtgtt ttgcggtacg tagttaacat cggcaaggct aattccaggc
    25141 gcgcttgcca gtaaccctgc tgataaacag agggcgatga gacttttatt cattttcatt
    25201 gttttcacct caaaatctgg agctcagcgg tagccaggca atagcgcgct aaacccgata
    25261 atcagagggg ctttcgcccc tttcagataa tgacaacctg tttttatgcc ggatgcggcg
    25321 taaacgcctt atccggccca ggttttgcta ttaccaccag atttccatct gggcaccgaa
    25381 ggtccactcg tcgctgtcgc cacgaccgaa gctgccgccg ttgaaatcag caggaacggc
    25441 tttgccgtag ttcgggttaa ccttgctatc gccgttgtag tcgtaacccc atttctcatc
    25501 ccacttggcg taggttgcga agacacgaat agccgggcgt gaccagatgc tgtcgccagc
    25561 ctgccattgt tgtgcgaggg taattttgta ctgattgttc ttgtcgccgg tgcgctggga
    25621 ttcgacgttg tcgtagccga tttccatcac ggtgctcatg attggcgtcc acttgtacat
    25681 cgggcgaata ccaacggtcc accacttggt gccgttgtcg ttatcccagt tgatatcctg
    25741 gtacataccc acgtacatca tgtcccagtt gtcgcccatg gagatcgcac cgtggtcgag
    25801 gatacgcagc atgtgaccgt tgttgttgat attgtaggca aatttttcgt tatcaaacgc
    25861 gacgccagaa ccctgcgaca gacctttacc ctgcgaggtc atcgagtcag tagcgtactg
    25921 aacaacaaac ttgttaaagc ccttcaggac actctgagta tgttcagcag tgaataacca
    25981 gccgtctttc gatgcgccat caaccagacg atagttatca cgcaggttgg cacgaccgta
    26041 gtcgacaccc agttctaatg tgccgcccgg gttgatttcc atctgcgcta aacgcacatc
    26101 gaaaacgtcg ttcgcggttt cgttggtata gtcataaata ttgttgctgg caaaagagga
    26161 agaaccacca gcttcagagg agcgggttgc tgccagagag agtttaccga agccaacatc
    26221 gatgttttcc agaccggcac caggaccaga aatatcccag tagtagaagt cgatcatatg
    26281 aacgtcatga cgttggtaga agcgcttacc tgcccagatg gtggaacctg gcagccattc
    26341 gatcaggttt tttccctgca cgtttgcttc acggaaggcc ggatcggtag cttcccagtc
    26401 attctgttgc gcgacggaat aggccacgtt agtgtcgaaa tagaagctct tatcgccctc
    26461 tttccacact tcctgaccca attttaattc agcataagtt tcacattcgt tgccaagacg
    26521 gtatttactt tgagcaccgg tagtctggaa acactgttgt tcaccgccgc tacctgtcca
    26581 gccaataccg gaacgtgcat agccgtggaa atcaacagcc attgcctgag cagacattac
    26641 gcccgctgcg acggcaaccg ccagaggaag tttgcgcaga gtaatcatca ttctatctcc
    26701 tgagtcattg cttttctttt ttcacatcac ctgtgacagg ctttgtgtgt tttgtggggt
    26761 gcttaaacac ccggctcctt atgcagtcga cgacatgcag tgccatcctc acggaacaga
    26821 tggcaacgct ctggcggcag gccgatagcg aatgtggcac cttcttctac caacgccacg
    26881 tcgttctggc ggtacaccag gttttgacga atggaaggga tctggatata gatttgggtt
    26941 tcgttgccga gttgctcgac gacctgaact tcaccctcaa ggatgacgtc agcgatatca
    27001 ctcggcagta gatgttccgg gcgaataccc agcgacatat tggctccaac ctggacatca
    27061 cggctttcaa caggcagcca gacttgttga cgatttggca tcggcagctc cacctgcact
    27121 tgatcgattg cggtggcggt cacttttacc ggcaggaagt tcatctttgg cgaaccgata
    27181 aatccggcga caaaacggtc tgccggatag tggtacagtt ccagcggttt cccaacctgt
    27241 gccacgcgac cggcctccag caccacgatt ttgtcggcca gcgtcatcgc ttcgacctga
    27301 tcgtgggtga cgtaaatcat tgtgcggccc aggcgtttat gcagacggga gatttcgata
    27361 cgcatttgca cacgcagtgc agcatcgagg ttggagagcg gttcatcgag caaaaatacg
    27421 cttggctcgg ccaccagcgt acggccaatc gccacgcgct gacgctgacc accggagagc
    27481 gctttcggtt tgcgatccag caaatgcgcc agttgcagca cttccgccac ctggttgacg
    27541 cgttggttaa tcacctcttt ttttgcgcca gccagtttca ggccaaatga catgttttct
    27601 gctactgaca ggtggggata gagcgcgtaa gactgaaaca ccataccaac gccgcgttct
    27661 gctggcggag tgtcattcat ccgtttctca ccgatgaaca ggtcgccgct ggtgatcgtc
    27721 tcaagcccgg caatcatgcg cagtaaagtc gatttaccgc agccagacgg tccgacaaac
    27781 accacgaatt caccttcatg gatatcgaga ttgatatctt tcgataccac gacctcgccc
    27841 caggctttcg ttacattttg cagctgtacg ctcgccatgc ccttctccct ttgtaacaac
    27901 ctgtcatcga cagcaacatt catgatgggc tgactatgcg tcatcaggag atggcttaaa
    27961 tcctccaccc cctggctttt ttatggggga ggaggcggga ggatgagaac gcggcttctg
    28021 tgaactaaac cgaggtcatg taaggaattt cgtgatgttg cttgcaaaaa tcgtggcgat
    28081 tttatgtgcg catccccaca ttaccgccaa ttctgtaaca gagatcacac aaagcaacgg
    28141 tggggcgtag gggcaaggag gatggaaaga ggttgccgta taaagaaact agagtccgtt
    28201 taggtgtttt cacgagcact tcaccaacaa ggaccataga ttatgaaaat aaaaacaggt
    28261 gcacgcatcc tcgcattatc cgcattaacg acgatgatgt tttccgcctc ggctctcgcc
    28321 aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc
    28381 gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg
    28441 gataaactgg aagagaaatt ccctcaggtt gcggcaactg gcgatggccc tgacattatc
    28501 ttctgggcgc acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc
    28561 ccggacaaag cgttccagga caagctgtat ccgtttacat gggatgccgt acgttacaac
    28621 ggcaagctga ttgcttaccc aatcgctgtt gaagcgttat cgctgattta taacaaagac
    28681 ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa
    28741 gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg
    28801 attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac
    28861 gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa
    28921 aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc
    28981 gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg
    29041 aattatggtg taacggtact gccgaccttc aagggtcaac catctaaacc gttcgttggc
    29101 gtgctgagca ccggtattaa cgccgccagt ccgaacaaag agctggcgaa agagttcctc
    29161 gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt
    29221 gccgtagcgc tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccacc
    29281 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
    29341 tatgccgtac gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
    29401 ctgaaagacg cgcagactcg tatcaccaag taatgctgtg aaatgccgga tgcggcgtga
    29461 acgccttgtc cggcctacaa aactgaaacg catgtaggcc tgataagacg catcagtgtc
    29521 gcatcaggca gttgttgtcg gataaggcgt gaaagcctta tccgtcctgg aatgaggaag
    29581 aaccccatgg atgtcattaa aaagaaacat tggtggcaaa gcgacgcgct gaaatggtca
    29641 gtgctaggtc tgctcggcct gctggtgggt taccttgttg ttttaatgta cgcacaaggg
    29701 gaatacctgt tcgccattac cacgctgata ttgagttcag cggggctgta tattttcgcc
    29761 aatcgtaaag cctacgcctg gcgctatgtt tacccgggaa tggctggaat gggattattc
    29821 gtcctcttcc ctctggtctg caccatcgcc attgccttca ccaactacag cagcactaac
    29881 cagctgactt ttgaacgtgc gcaggaagtg ttgttagatc gctcctggca agcaggcaaa
    29941 acctataact ttggtcttta cccggcgggc gatgagtggc aactggcgct cagcgacggc
    30001 gaaaccggca aaaattacct ctccgacgcg tttaaatttg gcggcgagca aaaactgcaa
    30061 ctgaaagaaa cgaccgccca gcccgaaggc gaacgcgcga atctgcgcgt gattacccag
    30121 aatcgtcagg cgctgagtga cattaccacc attctgccgg atggcaacaa agtgatgatg
    30181 agctccctgc gccagttttc tggcacgcag ccgctctaca cactcgacgg tgacggcacg
    30241 ttgacgaata atcagagtgg cgtgaaatat cgtccgaata accaaattgg cttttaccag
    30301 tccattaccg ccgacggcaa ctggggggat gaaaagctaa gccccggtta caccgtgacc
    30361 accggctgga aaaactttac ccgcgtcttt accgacgaag gcattcagaa accgttcctc
    30421 gccattttcg tctggaccgt ggtgttctcg ctgatcactg tctttttaac ggtggcggtc
    30481 ggcatggttc tggcatgtct ggtgcagtgg gaagcgttgc gcggcaaagc ggtctatcgc
    30541 gtcctgctga ttctgcccta cgcggtgcca tcgttcattt caatcttgat tttcaaagga
    30601 ttgtttaacc agagcttcgg tgaaatcaac atgatgttga gcgcgctgtt tggcgtgaag
    30661 cccgcctggt tcagcgatcc gaccaccgct cgcacgatgc tgattatcgt caatacctgg
    30721 ctgggttatc cgtacatgat gatcctctgc atgggcttgc tgaaagcgat tccggacgat
    30781 ttgtatgaag cctcagcaat ggatggcgca ggtccgttcc agaacttctt taagattacg
    30841 ctgccgctgc tgattaaacc gctgacgccg ctgatgatcg ccagcttcgc ctttaacttt
    30901 aacaacttcg tgctgattca gctgttaacc aacggcggcc cggatcgtct tggcacgacc
    30961 acgccagccg gttataccga cctgcttgtt aactacacct accgtatcgc ttttgaaggc
    31021 ggcgggggtc aggacttcgg tctggcggca gcaattgcca cgctgatctt cctgctggtg
    31081 ggtgcgctgg cgatagtgaa cctgaaagcc acgcgaatga agtttgatta agggagataa
    31141 caaaaatggc aatggtccaa ccgaaatcgc aaaaagcacg tttgtttatt actcacctgc
    31201 tactgctact ttttatcgcg gcgattatgt tcccgctgct gatggtcgtc gctatctcgc
    31261 tgcgtcaggg aaacttcgcg accggtagcc tgatcccgga gcaaatctcc tgggatcact
    31321 ggaaactggc gttaggtttt agcgttgaac aggctgatgg tcgcattacg ccaccgccat
    31381 tcccggtact gctgtggctg tggaactcgg taaaggtcgc tgggatttcc gcgattggca
    31441 ttgtggcgct ctccaccacc tgcgcctacg ctttcgcccg tatgcgcttt ccaggcaaag
    31501 cgacgctgct gaaaggaatg ctgattttcc agatgttccc ggcagtactt tcactggtcg
    31561 cgttgtatgc gttgtttgat cgtctgggtg agtacattcc attcattggc ctgaatactc
    31621 acggcggcgt aattttcgcg tatctgggtg ggattgcgct gcatgtctgg accatcaaag
    31681 gctatttcga aaccatcgac agttcgctgg aagaagcggc tgcgctggat ggtgcgacac
    31741 cctggcaggc cttccgcctt gtcctgttgc cgctgtcagt accgattctg gcggtggtat
    31801 tcatcctgtc gtttatcgct gccattactg aagttccggt cgcgtcgctg ttactgcgtg
    31861 acgtaaacag ctacaccctg gccgtgggga tgcagcaata cctcaacccg caaaactacc
    31921 tgtggggtga ctttgccgcc gctgccgtga tgtctgcatt accgatcacc atcgtcttct
    31981 tgctggctca acgctggctg gtcaacggcc tgacggcagg tggtgtgaaa ggttaaagat
    32041 gttgttctgc caatgttatg ccgctgcacc ctcaacttac gttatcccaa cttgtgacta
    32101 ttattcggcg ctccacggag cgcctttttt tctttcgtct gcaatctgaa tcgttcgccg
    32161 gttaatattc ccatcataga acttattatt tttacgttat ttgttttccc acttacgata
    32221 attctctttc gtgctctgag tcacggcaat agtattgttt tcatcaattt ttgataatta
    32281 tcacaattaa gatcacagaa aagacattac g
//
LOCUS       DADMFL010000039        31895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.039, whole genome shotgun sequence.
ACCESSION   DADMFL010000039 DADMFL010000000
VERSION     DADMFL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.039"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            204..1892
                     /locus_tag="JNZ27_001771"
                     /pseudo
     CDS             204..1892
                     /locus_tag="JNZ27_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            1907..2803
                     /locus_tag="JNZ27_001772"
     CDS             1907..2803
                     /locus_tag="JNZ27_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY8664134.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            2836..3084
                     /locus_tag="JNZ27_001773"
     CDS             2836..3084
                     /locus_tag="JNZ27_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8664135.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            3084..4256
                     /locus_tag="JNZ27_001774"
     CDS             3084..4256
                     /locus_tag="JNZ27_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8664136.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4292..5371)
                     /gene="lptG"
                     /locus_tag="JNZ27_001775"
     CDS             complement(4292..5371)
                     /gene="lptG"
                     /locus_tag="JNZ27_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8664137.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5368..6438)
                     /gene="lptF"
                     /locus_tag="JNZ27_001776"
     CDS             complement(5368..6438)
                     /gene="lptF"
                     /locus_tag="JNZ27_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8664138.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6469..7029)
                     /locus_tag="JNZ27_001777"
     CDS             complement(6469..7029)
                     /locus_tag="JNZ27_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8664139.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7041..7865)
                     /locus_tag="JNZ27_001778"
     CDS             complement(7041..7865)
                     /locus_tag="JNZ27_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664140.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(7865..9214)
                     /locus_tag="JNZ27_001779"
     CDS             complement(7865..9214)
                     /locus_tag="JNZ27_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY8664141.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9260..10018)
                     /locus_tag="JNZ27_001780"
     CDS             complement(9260..10018)
                     /locus_tag="JNZ27_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664142.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(10050..10763)
                     /locus_tag="JNZ27_001781"
     CDS             complement(10050..10763)
                     /locus_tag="JNZ27_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8664143.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            10937..11629
                     /locus_tag="JNZ27_001782"
     CDS             10937..11629
                     /locus_tag="JNZ27_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8664144.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11678..13177)
                     /gene="pitB"
                     /locus_tag="JNZ27_001783"
     CDS             complement(11678..13177)
                     /gene="pitB"
                     /locus_tag="JNZ27_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY8664145.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13469..15328)
                     /gene="gss"
                     /locus_tag="JNZ27_001784"
     CDS             complement(13469..15328)
                     /gene="gss"
                     /locus_tag="JNZ27_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY8664146.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15533..16399
                     /gene="yghU"
                     /locus_tag="JNZ27_001785"
     CDS             15533..16399
                     /gene="yghU"
                     /locus_tag="JNZ27_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY8664147.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16522..16770)
                     /gene="hybG"
                     /locus_tag="JNZ27_001786"
     CDS             complement(16522..16770)
                     /gene="hybG"
                     /locus_tag="JNZ27_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY8664148.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16904..17245)
                     /gene="hypA"
                     /locus_tag="JNZ27_001787"
     CDS             complement(16904..17245)
                     /gene="hypA"
                     /locus_tag="JNZ27_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8664149.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17238..17726)
                     /gene="hybE"
                     /locus_tag="JNZ27_001788"
     CDS             complement(17238..17726)
                     /gene="hybE"
                     /locus_tag="JNZ27_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY8664150.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(17719..18213)
                     /locus_tag="JNZ27_001789"
     CDS             complement(17719..18213)
                     /locus_tag="JNZ27_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY8664151.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18213..19916)
                     /gene="hybC"
                     /locus_tag="JNZ27_001790"
     CDS             complement(18213..19916)
                     /gene="hybC"
                     /locus_tag="JNZ27_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY8664152.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19913..21091)
                     /gene="hybB"
                     /locus_tag="JNZ27_001791"
     CDS             complement(19913..21091)
                     /gene="hybB"
                     /locus_tag="JNZ27_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY8664153.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(21081..22067)
                     /gene="hybA"
                     /locus_tag="JNZ27_001792"
     CDS             complement(21081..22067)
                     /gene="hybA"
                     /locus_tag="JNZ27_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY8664154.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(22070..23188)
                     /gene="hybO"
                     /locus_tag="JNZ27_001793"
     CDS             complement(22070..23188)
                     /gene="hybO"
                     /locus_tag="JNZ27_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY8664155.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23377..23664)
                     /locus_tag="JNZ27_001794"
     CDS             complement(23377..23664)
                     /locus_tag="JNZ27_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY8664156.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23782..24708)
                     /locus_tag="JNZ27_001795"
     CDS             complement(23782..24708)
                     /locus_tag="JNZ27_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8664157.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            24826..25866
                     /gene="gpr"
                     /locus_tag="JNZ27_001796"
     CDS             24826..25866
                     /gene="gpr"
                     /locus_tag="JNZ27_001796"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY8664158.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25906..26400)
                     /locus_tag="JNZ27_001797"
     CDS             complement(25906..26400)
                     /locus_tag="JNZ27_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY8664159.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFMMGYLDRLT
                     RHNH"
     gene            26591..27475
                     /gene="yghA"
                     /locus_tag="JNZ27_001798"
     CDS             26591..27475
                     /gene="yghA"
                     /locus_tag="JNZ27_001798"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8664160.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27747..28172)
                     /gene="exbD"
                     /locus_tag="JNZ27_001799"
     CDS             complement(27747..28172)
                     /gene="exbD"
                     /locus_tag="JNZ27_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY8664161.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28179..28913)
                     /locus_tag="JNZ27_001800"
     CDS             complement(28179..28913)
                     /locus_tag="JNZ27_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY8664162.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29165..30352
                     /gene="metC"
                     /locus_tag="JNZ27_001801"
     CDS             29165..30352
                     /gene="metC"
                     /locus_tag="JNZ27_001801"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY8664163.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30492..31151
                     /locus_tag="JNZ27_001802"
     CDS             30492..31151
                     /locus_tag="JNZ27_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8664164.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31191..>31895)
                     /locus_tag="JNZ27_001803"
     CDS             complement(31191..>31895)
                     /locus_tag="JNZ27_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8664165.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT         7932 a         8439 c         7910 g         7614 t
ORIGIN      
        1 ttcataaatt aaatatcatt cataataaaa ataaaaaact caaactcatt atttaaatca
       61 atgttcattt tcacaccgat gaaaatacta tctcatttat cttttatgcc tatataatcc
      121 accgcattat tgtcatttta tttttgatat ccaatttttt cgtggtgtat atgtctaata
      181 aaatctttac gcattcccta cctatgcgct atgccgattt tccaacgctg gttgatgctt
      241 tggactacgc cgctctgagt agagccggaa tgaattttta tgacagacgt tgccaacttg
      301 aagatcaact ggaatatcag acattaaaaa cgcgtgccga agctggtgcg aagcggttgt
      361 tatcgctgaa cctgaaaaaa ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt
      421 ttgtagaggc tttttttgcc tgtcagtatg ctggcttagt cgccttcccg ttggcgattc
      481 caatgggcgt cggtcagcgg gattcctgga gcgccaaact ccagggttta ctggcaagtt
      541 gccagcccgc agccattatc actggtgatg agtagttgcc actggtcaat gccgcgacgc
      601 ataacaaccc cgaattacat gttttaagcc acgcttggtt taaggcatta ccggaagccg
      661 atgttgcgct ccagcgtcca gttccgaacg atatcgccta cctccagtac acctccggca
      721 gcacccgttt tccccgtggc gtcattatca cccatcgcga agtgatggct aatctacgtg
      781 ctataagcca cgatggcatt aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt
      841 tctaccatga tatgggactg gtcggctttc tcctgccccc cgtcgccacg caactttcag
      901 tagattattt gcgcactcag gattttgcca tgcgtccttt gcaatggctt aaattgatca
      961 gtaaaaatcg cggcaccgtt tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc
     1021 gcgtgaatga aaaagatctc gctgaactgg atctttccta ctggcgcgtc gctggtattg
     1081 gtgctgagcc gatctcagca gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta
     1141 actttgacaa taaaactttc atgccgtgct acggactggc agaaaatgcg ctggctgtca
     1201 gcttctctga tgaagcctcc ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat
     1261 atcagggtaa agccgtcgcg ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact
     1321 gcggcaaagc gttgccggaa catggtattg aaatccgcaa tgaagcaggt atgccggtcg
     1381 cggaacgtgt ggtaggccat atttgcatct ccggtcccag tctgatgagc ggttactttg
     1441 gcgaccaggt ttcgcaagac gagattgccg cgacgggctg gttagacacc ggcgacctcg
     1501 gttatctgct ggacggttat ctgtatgtca ccggacgcat taaagatctg attattattc
     1561 gtggccgtaa tatctggccg caggatattg aatatatagc ggaacaggaa ccggaaattc
     1621 attctggcga tgcgattgct tttgttaccg cccaggaaaa aatcattttg cagatccagt
     1681 gtcggatcag cgacgaagaa cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc
     1741 aaagcgaatt tggcgtgacc gcggctatcg atctgttgcc gccccacagt attccccgaa
     1801 cgtcctccgg caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg
     1861 ccagtcttaa tgtgcaggaa tccctggcat gaatcaaact gtcgcggtga cgggcgctac
     1921 cgggtttatc ggtaaatata ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc
     1981 attgacgcgt actgcccgcg ctcacgtcaa tgataatctc acctgggtgc gcggttcgct
     2041 ggaagatact cattcactta gcgagctggt tgccggagcc agcgtagtgg tccattgcgc
     2101 cgggcaagtg cgcgggcaca aagaagagat tttcacccgc tgtaacgttg acggcagcct
     2161 gcgcctgatg caagcagcaa aagagagcgg cttttgccaa cgttttctgt ttatctcttc
     2221 gctggcggcg cgccatcccg agctctcctg gtatgcaaaa tccaaatacg tcgccgagca
     2281 acgactggca gcaatggcgg acgaaattac gctgggcgtt tttcgcccga cagccgtgta
     2341 tggtcccggc gataaagagt taaaaccgct gtttgactgg atgctacgcg gcctgctgcc
     2401 acgacttggt gcgccagata cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc
     2461 agtgggtcag tggttaagcg ccgaaactgt acagacgcaa acctatgaat tatgcgatgg
     2521 cgtcgccggt ggctatgact ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg
     2581 ttccgtgcga atggttgaca ttcctctgcc ggtactcaca ggccttgcgg atatcagtac
     2641 cgcgttgagt cgcctggcag gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt
     2701 aacccacgcc gactggtcgg caagtaataa ccgtatttct gaagatatca attggtttcc
     2761 cgggattagc ctggaacacg cattacgcaa cgggctattt tgattgtctt gttttaaagg
     2821 tgtgttaacg tttctatggt aaatcgtgaa atagtaatgg attacatcct ttcctgttta
     2881 caggatttgg tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt caacgatctt
     2941 ggcctggagt cgatcaaagt gatggatctc ttaatgatgc tggaagacag attcgatatt
     3001 tccattccta ttaatattct gctgaatgtc aaaccccctg cgcaattaat ggaaactcta
     3061 cttccatggc tggagaataa ataatggggc tatacgataa atatgcgcgc cttgctggcg
     3121 agcgtctgca atttagtgac aacgggttaa caccgtttgg cacctgtatt gatgaagtgt
     3181 attccgccac cgaaggccgg atcggcaata aaaaagtgat tctggccggg accaataatt
     3241 atctcggttt aacctttaat catgatgcca tcagcgaagg tcaggccgcg ttagcggccc
     3301 aaggcaccgg caccaccggt tctcgtatgg caaacggcag ttatgcgccg cacctggcgt
     3361 tagagaaaga gattgccgag tttttcaatc gccctaccgc tatcgttttt tcaaccggtt
     3421 ataccgctaa tttaggcgtc atcagcgcac ttgccgacca taacgccgtg gtgttactgg
     3481 atgccgacag ccacgccagt atttatgacg cctgttctct cggtggcgcg gaaattattc
     3541 gtttccgcca taacgatgct aaagatctgg aacggcgcat ggttcgcctc ggtgaacggg
     3601 cgaaagaggc gattatcatc gttgagggca tctacagcat gctcggtgac gttgcgccgc
     3661 tggcggaaat tgttgatatc aaacgtcgtc tcggtggtta tttgattgtc gatgaagcgc
     3721 attcgtttgt cgtgttaggc gcaacggggc gcggactggc tgaagctgtc ggcgttgaag
     3781 atgatgtgga tattatcgtt ggtactttca gtaaaagtct ggcctcgatt ggcggttttg
     3841 ctgtcggttc ggaagcgatg gaagtgctgc gctacggcag ccgcccgtat atttttaccg
     3901 cctctccttc tccgtcctgc attgcgaccg tgcgctcgtc gttaagaact attgccacac
     3961 aaccagagct gcggcaaaag ctgatggata acgccaacca tctgtatgac ggtctgcaaa
     4021 aactgggtta tgagttaagt tcacatatta gcccggtagt gccagtaata atcggttcga
     4081 aagaggaagg tttacgcatc tggcgcgagc ttatctcgcc gggcgtgtat gtgaatctta
     4141 tcctgccgcc tgccgcgcct gcaggcatca cgctgctgcg ctgtagcgta aatgctgccc
     4201 acagtcgtga gcaaatcgat gccatcattc aggcatttgc tactctgaag cagtaatgtg
     4261 agcccgccgt ggtgtttgcg ccacggcggg tttaatgttg tctgtcatac caatacacca
     4321 ggctcaacgc caccaacaca aacgctaccg gtaaaccgag cgcggtaaca ggtgcgctta
     4381 acgcaaatag caatcccaga ttaaccatga tttgatagct gatccaggtg agtaagccca
     4441 cgatgacgcc tacagcgaga cggctaccca tcccaggcga acgcggggca ctaaaggtaa
     4501 acggcacagc cagcaaaatc atcgccaggg tcaggatggg gtgccccagt ttttcccaca
     4561 gagcaaggcg gtattcgctg ctgggttgcc cggtttcctg caaataatgg atgtaatgat
     4621 taagctgacg gacagaaaaa gtattccccg gcattgataa ctcttcaaga ttcatactgg
     4681 tgaaggccga ttgccaggca agattatcta atgtttccag cgtttctttg ccattcagcc
     4741 attttttatg attcacacca tgcaggaccc acgttttgtc gtcttcaatg gtggcactgc
     4801 gtgcataaat gtaggattca agcgagagat cgtcgcgata atgaaatatc tccacgccca
     4861 ccggctggcc ctgctcgttc agcgatttca ctgtcacaaa ttcattacca cgcctggccc
     4921 acagcatatt gccggtaatg tcatcgtcct cccccaacgc ggtggcggtc gatttgattt
     4981 gcaacgcctg ttgctgcaat ggcgacgcca cccactcatc tatcgcgcct aacgaaacag
     5041 tccacaatat cccggcaacc agcgccacca gtgcaatacg gaagatagaa aaccccgtgc
     5101 tgcgaatggc ggtaagctca ctgtttttcg ataactgccc cagtccaaca ataccaccca
     5161 ataacgcgat aaacggcgaa agttcgacca gtgtgcgcgg taaggtcatt agcaccacca
     5221 gcaccgcctg agtccagcga taaccgcccg ggctgacacc atccagttcg ttaatcaggt
     5281 tgaaggtggt aaaaagcggc agcaatagcc ctgcagcggc ggcaaaaccg agaaagagat
     5341 gacggattaa atagcgactg aaaacattca tcgggaaaat ttctgcagca aagagaagtc
     5401 acgtgcgacc agtatcagca ggcccattag catgagtccg ggcactaacc acaaaccagg
     5461 aatgagggga atcgcgccgt tagccaccag tgtacggcag acgtcgccgc cataaaaaat
     5521 ggcaataaac agcaacgtca gtggcaataa cgttgaaaat cgcccttgcc gcggttttac
     5581 ccgacttaat gaaatggcca gcagcgccat caacaatgcg gtcaggccac ggctttgtcg
     5641 ccattgcagt tcggcatgat cggcaggaaa ggcggagcgc gccagctccg tgaccgattt
     5701 cgctttgcgt ttgacgttcg ggctttgatc cagcggattc agatgtaatt gcagattacg
     5761 gtagatctgt tcgttgtcgt cacgcccctg atgatcgaga agataggcag tgccgttgtg
     5821 caacataacg gtcggtttct cgggtgatgg gtcaaccaca tcaaccgaac gggcgcggaa
     5881 aatacgggtt cgattggcag tagaagtata aatcagcgcg tcagtcaggc gattattatc
     5941 ctgatcaacc gtctgcgaaa ggatcattcg tccgttatcg ttagtgttaa atttctttgc
     6001 ccgcaactgg cgaacatcca gctccgactg cgactgctgc tccagttgat aaatctgcgc
     6061 ataagcccac ggacgaccat acatcgacag aagggtgaca aaaatactta gcggtatcgc
     6121 cagataaaga acggctttgt acaagcgtcc cggactgccc cccgccgcag agatagcggt
     6181 aatttccgag tcggtataca tctgccctag cgtcacgcca actgacacat acagaccaac
     6241 aggtaacaac atctccagtg caatcagtac tttgtagaaa acgatatcca gcacaacatc
     6301 aagagctaat gtcccatttg ccgcttcggt cagataacgc tgtgcggagt agctggcgaa
     6361 aataaagatc agaaaaccga cgataatcag caccagacgg cgcgtaccac gcatgatgta
     6421 gtgttcaacc agcttcatct cactaacgcc ctcaatcctt tgagtttttt attcattgat
     6481 cagtttctgc gccaacgcaa tgacatgatc tacctcttcc ggcgtcaggc tgccgtcctt
     6541 tgcccactga atttgcccgg ctttattcag caccagaatg gttgaactct gctccggcaa
     6601 gcgccaggca acgcgtccct gtccattgcc gtcaataacg aattgcgccc acggatagcg
     6661 gcgtttattt ttttcgattt tgccaacaac aaaatagcca gtaccaaaga tggcatcatc
     6721 ggtgttcaca atggtggtgg gctgaaaacg atcctgcgga aagtttgccg cttcaaccgc
     6781 tttaattaac agtgagttct ttttcttcgc cgacttacgc ccggcgatat actgaataat
     6841 gcggactttt cccgctaatt gtgtgctgtt ccacttttgg tagtgaattt cgtcgtcgct
     6901 attcagaagc agctcgccgc cttcctggat ataaacaggg gtaaccgttt tgccagtgac
     6961 gaaattgtgt gcctgcacac ccggggctaa gagcatgata atcaacgcaa taatcaaacg
     7021 agatgacatc attgctccgg ctatagcgga aaacgcgaaa tcagcagctc taagcgctga
     7081 caaatatcgc tatccagcgc caacggtgca taatacgact ggcggttttc catatcatcc
     7141 gccaccatgt cgtcgtgttt atctcccatc aggccattaa tggcggcgag gaaacgggaa
     7201 atctgtaacg cctctttatt ttcaggatca agcgatctta aggtgtcgta ataagaaacc
     7261 tgctggctga tgatttttcg cacatcatga aattccctgc cggttagtcg cggtttggca
     7321 ataatctctt tcagggtatg catttgcttt tgccgatagg caataaaact gcttacgctt
     7381 tctaactggc tatggcgtaa tgaatagttc accaacgacc agaccggaga gcttaaataa
     7441 atccgtaaca ggttgccgta atacgacaca atatttttct taccgttacg aaagccgtcc
     7501 tgaaaacgcc ccagaaaaac ggtcgttttg ccgaataaaa atccggcctg atgcttcttt
     7561 aaatacagcc gttgcgcaaa acgcaggtgc ttgtagcgcg ctcgcatgta tttaaattgt
     7621 ttgcgctcat cagcagtcag ctcatcgcct ttggcttgtt tgcgaactaa acgcagtaat
     7681 tcctgacgat caacgccatt gagccagaat tgcaggcata acgcgtaatt atcatgaatg
     7741 atttcggtgg tacaacttag tgcaatagtc ggcggcaaac taacctgtgc atcaacgaca
     7801 tcatcttctt caatcgcaac aaatagacgc tcgctcgcct caagcgggaa atattttctc
     7861 atcatcagga tttctctccg gctgcgttat aaagcaagga atggcgctga aaaaatgcct
     7921 ttaaggttgg aattaacgag agcgccacgt ccagacattg cccaccgaag taagcaaacg
     7981 ctgctcccag cacaccaaac agatgacaga acatgaacag caatacgatg tagccgattg
     8041 aggcgatggt ttgcgccacg agaaaagcgc gctgttttcc cgccatcaat agcaatgact
     8101 cctgcgggaa gcccagcatc gaaatcacaa ttgcgcccag catcacctga atcaggtcat
     8161 acgcttcgag atacttaacg ccaaacacca gcgaaatgag cggcttgccg acgatcaaca
     8221 ccgcgagcgc caccagaata ccgattccgc ccgccagtaa accggacttc acacctaaca
     8281 accacggtct ggtggtgcgc ggatctaaac gcatcacctc tgggtaaaaa cttttaccca
     8341 gcaaacccgc tggcgttccg gcggcgtcga agaatgtcat ggcgatttta aataacccag
     8401 cggcagcggg tcctaacacg atccccacca acactgtgct gcacgagtta cgcgccgacc
     8461 agatggagtg ggcaatgttg gttgaccaga caaaactcca cgcgccttta atgtgtcggg
     8521 cagactcaaa cagattcaat ttgaaggcgt tatggatatt tctacggcgt aattcgcgcg
     8581 cggcaaacca ccagtacatg gtgccgccaa ccagattcga cacgtaccag gcaataacaa
     8641 aacccgcaaa accaaagtca aaataccagg ctgcgacgct ccccgctgcg cgcagaaaag
     8701 gtttcgtcgc ctgctgtaca gcaatcaaat caaagcgatc taccgcacgc agaatgccgg
     8761 tcggcgtgga ggaagccatt gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc
     8821 tttggtcatc cagacctaat gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa
     8881 tcgccaccgc gccgctgacg atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt
     8941 gctgcggatt attgttggtc aaggctggcg ttccgtactg aaccaccagc tgccatgtct
     9001 gaaacttaat aaaatcgctg atcgacttgg cgtacgattg cacaatcacc agtacaccaa
     9061 acatggcggg cgtcatccct ttaccggcac acgagagcgc caacagcccc agcaaggcgc
     9121 tctcgacatt actggagcct aaccaggcgc tattgcgaat aatggtgcga aacgcgccat
     9181 ctgcaaacca atgtttgatg ttaaaacccg ccaattcagc ctgaccttat cgttgatagt
     9241 aaaaaatatc ccgccagcct taagttaaac ttcggcggtc agaaacgatg gcaaccagag
     9301 aaaccgcctt ctgtacctgt tccagcactt cgctgtaggg cgctctggaa tcaatctcaa
     9361 gaatttttgt gccgttatag ccaatcgtcg acatgacgcc gattttgtcc tgcagctcgg
     9421 catactcatg gtcaggcttg cgggagatgg cagtatcaat atcaatcccc aaacgaatga
     9481 ttaattcagg acggtattgc gccatttttt ggtataaact ccgttcgcgc tgcgccagaa
     9541 acatgctgat tttcccggtc gcacgttcga cgccgatccc cggtccgtca taataaaagc
     9601 ccgaaatttc agcctgcggg aagcgatcgc tgaccaccag aacgccactt tgcgccagtc
     9661 gctgaacctt gcgtagattc gccattcttc gcagcgagaa gcagtacata atcaccgccg
     9721 cccatagcgc cggagatttg gttttcatgc tttgggtttt cgaggatttg gccgccagtc
     9781 gccgttccag ccagacgcca accaacggca atcgtttgat tttgtcgccg tcttcgccgg
     9841 agagcagccc cagatagcgg cgctcggttt gccagtgttg ttgcagcgat ttcaccaggt
     9901 cggtggtcag cgtggattta ccggtgccat cacacccgac caccgcaatc agccccggaa
     9961 tgtagttggg ctgcggcgcg gttgtactat taacagtttg tgtttgtagt gcatccattc
    10021 gttaaatcat cccctatcca ttttggtgat caggaaagcg cggcgtgaat tgcgcgtagt
    10081 gacgcttgca taatttcatc ggctggttgc cgaccatcca gttcaaggat cttggcgcca
    10141 ttgaatgtca gttgcggcgt aacggcgatt ttttcctgta acgctgccag ttggtggtca
    10201 ggtttacgcg caaacgcggt ttgttcatca atgccaagac gaatcaacaa tacgggtaaa
    10261 taagacgcca tccattggta cagcttcaac tcgcgctgcc ttaacatttt tatccaaccg
    10321 ttaccgcccg tggtttttgc caattgcggg ccatcaaagc gaaaacccgg cacttcaacc
    10381 tgcgggtagc ggtcggtgat gagcagatag ccttgctggc ttttacacaa cattttgcga
    10441 aacttgtacg cccgccagca ggaaagcaga tagataacca gcgcagtaat attgccaggc
    10501 ggtgttgagg gcttttcgtg tacatgtgcc gctttacttc gcagataaca cccacaaggt
    10561 gcgccaataa cagggagctg tgaaatccat tcgccaattc gcccggacga ttgcccgaga
    10621 taaatgtgtt ctgttggcat tcttgctgcc agttcattta ccaggcttgc cgtgagggtc
    10681 gatttacctg aaccatcaca tcctacaatg gcaataacac gcactggcgt ggaattaatt
    10741 attgacatat tacgttgatt cacgaaaaac ccggcagtaa ataatgtatt gaatattacg
    10801 tggtccgtgc tcagaatatc cgttcaacct tattgtgaca atggttaatt gtgacattga
    10861 cctgaattac gctttcataa aaacatatta accaaataaa tatttttaat ggatatttaa
    10921 attaaaggat atattcatgc agtcaataac acctccatta attgccgtta ttggtagcga
    10981 tggttcaggc aagtcaacgg tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc
    11041 tgccgaaaga gttcatttag gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt
    11101 acctttgatg ggaaaatcct tacataaaac aattgaacga aatcaggtga aaacagcaaa
    11161 aaaattgcct ggaccagttc cggcgctggt aattacagcg tttgtcgccc gtcgcttact
    11221 gcgctttcgt catatgcttg cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta
    11281 tcctcaggac caaattcctg gcgcttacga tggtacggtg ttcccaccta acgttgaagg
    11341 tggtcgtttt gtctcatggc tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag
    11401 ccataagcct gatctggtca tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa
    11461 acccgaccat aaacgggaat cgctggcgag gaagattgcc ataacgccac agttaacctt
    11521 tggtggtgca caactggttg atatcgatgc caatcagcca ctggaacagg tgttggttga
    11581 tgcagaaaaa gcgattacgg attttatgac cgcgcgtggt tatcactagt caaaaatgga
    11641 aatgcccgat cgccaggacc gggcattttc aggaaggtta aatcaactgc aatgctatcc
    11701 agtacagccc accagaaaga aaaattgccg ccggtaaagt aaacacccac gccatcagga
    11761 tgctggttac cgttttacgc tgtaacccac cgccgtccac caccatcgtc cctgctaccg
    11821 cagacgagag gacgtgtgtt gtggagacag gcatcccaat ataactggca aggccgatag
    11881 acactgccgc cgtcatttgt gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa
    11941 tcttctcacc gatggtcatc gctacacgac gccagccaat catggtgcca atgccgagcg
    12001 ccagtgctac cgccatgatg atccacaccg gagcgtactc aatggtgctt aacatatcgc
    12061 tgcgcagttt tttcagcagg ttctggtctt ctttactgac gcctggcagt ttcgccagct
    12121 tcgcggaggt atcagagatg cacagcataa tgcggcgcag ctggctgcgc tgactcacgc
    12181 ttaacggctc gtaactttcc atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat
    12241 caaaggtatt tgccggatga cagtgaaact ctgttacttg cgtgccatca gtcgatgctg
    12301 caggcaatgg aggttccatc gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt
    12361 agtgttcgaa gttggtaacg gcatcgcggg tacgggtaat ttcatagccg gacgcattca
    12421 tattgacgac gaagccagca ggggcaatcc ccaccagtac cagcattacc aggccgatcc
    12481 ctttctgccc gtcgttcacg ccgtgcgaaa acgccacgcc tgcagcggaa acaatcagcg
    12541 caatacgcgt ccagaatggc ggtttacgtt tgcccttttt ctttttgcga tcttccggaa
    12601 tgcggtgaat acggtcacgc tttttcgtcc cactccagaa acggcgcagc aggaaaatca
    12661 gaccacccgc aatgaccagg ccgacgatag gggaaacaat cagcgaggag aaaattttgg
    12721 ttacttcacg caggtttaac gcatccatca ccgatgagcc ggttaacagc gcgttggtta
    12781 aaccgatgcc gataatcgca ccaatcaagg tgtgcgaact ggaggccggt aaaccaaaga
    12841 accacgttcc caggttccag ataatcgccg ccagcagcat ggaaaagacc atcgccagac
    12901 cgtgagttga ccccatattc agcaacaaat cggttggtaa catatggaca atggcatagg
    12961 caacgctaag tccgcccaat aacacgccaa aaaagttaaa aaatgccgcc atcaccacag
    13021 caagttgtgg ctgcatggca cgagtataaa taacggttgc caccgcattc gccgtgtcat
    13081 gaaaaccatt gattgcttcg tagaacaaca caaatgccag agcaagtaat aacaaaagcc
    13141 ccgtatatat atcaaggcca acaaataaat ttagcatata ttagattacg ccattttgaa
    13201 tttacgaacg gacgcattat cagtgacttt aacggcatgg gcaaagtgaa atatcatttt
    13261 ttaacgataa aaagagtgtt atttcttgtg tattagacaa taagcattca ttcaaaacct
    13321 ttagaaataa tcaaaacaca ggtattccac tggttaatat attcacatat gaaatgaata
    13381 aatattggaa tatataaata ttgaatattt tgattaatcc ccgtactaat tattcttcat
    13441 aatcagtacg gggttgctac aacatgtatt actttttcac cacaattaac ggttcaatat
    13501 cactctcttt tttgatgacc agtgattcat caccgcgcaa acacgtccca ccgtagttgc
    13561 cgccaacggt gaaggtacat acctgaatgt atttaccgtc cactttcggc aaacaccaca
    13621 gttgctgata gatgtttttc tgctcggcaa atttaccgct ggttttgtcc agcacctctt
    13681 catgatggcc gaccaggtcg atattgctac cacagcgacc ggcgatcggt ttcactgcgt
    13741 aacctgtttt caccaattca tcattaacag tgaaatcggt atccagcagg taacgatggt
    13801 gcgggaacag cgaccagagg atcggcagaa tcgctttgtt gccggggatc accgtccaca
    13861 gcggctcaaa gaccagcact tccgggcgca gcaatacgtc gataagacgc acttcgtttt
    13921 gcggatgacc ggtacggatt ggcaccgcag caaactcacg gtcgctaact tcacgaatct
    13981 gatcaaacgc ggtttcccac gcccaggttt tccacacgca gttaaccagt cgcccttccc
    14041 catcaatcag ttgcccggca gcatcccagc ccagttcatc cagcccgcgc aagatacgcg
    14101 tttcaaagcc cgcctggtgc agcgcctgct ccataaactg cgcgtgatag ttttcctcga
    14161 tatctttgtc ctgcatgata tggacaaacg gacgtgcacg actgtgtttc caggcaccag
    14221 ccagttcgtt aatcagccct tccgccggat tgaagccgtt gcctttatag ccctgctccg
    14281 cccaacgttc gaggatcaag cccgcttcgg tatgacagga ggcggagtcg gcgttgtatt
    14341 cgtaaacctt caggccacgc tcatccatgc agaaatccat acgaccagtg atcatatggt
    14401 gacggcgacg ctgccaggag agacgcaaac gtggccagag gattttcgga atgtcgaaca
    14461 gcgccagcag gttgtcatct ttcagcacct tgtcggttgc gtgaagatac atcagatgca
    14521 gctcgttggt ggctttaatt agctcctgct cggcactctc ggtaatggtg tagtaatgat
    14581 acggatcctg attgatcacc tgaccgttgg cctgcacata ggcgttttgt agcggatctt
    14641 tttcatccag ccatttaccg tcaaactggc ctttgttttc cagacgcgct ccactgattt
    14701 tcagcagctc gcctgcaatt tccggctgcg gtaagctgta ttcagtatct tccgtctgga
    14761 tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt aagggtatag cagccgtttt
    14821 cgaccaccat ctccagctcg cgcgtccact gttgcccttg cggcaacggg gaatgaatca
    14881 cgttctgttc cgcaatacgg actttgttgc catgcaattg ggtaatgatg gcgacatggc
    14941 cagtgtcttt aaattcaccg cctttatccc agataagaag cgcacccgcg accggcgcac
    15001 gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt gtcattaacc acttctcgca
    15061 ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc agtaaagacc acaccgtaat
    15121 tcagaaagag aaaacggcga gcaaattcaa cgcattgcca cttgtggccc atatattcgt
    15181 cgtcgatata gctacggaat acggcgtcat cttcgtattc ctgcggatcg agagaactgt
    15241 aatctgaaga gtagattgct accccacctg gggcgtagcc caataatgtc ccgaacgggg
    15301 catcctggct ggtcgttcct ttgctcatca ctttaccttt aacaatacaa cctaagcagt
    15361 tggcgtaatt gttgtgctct gattacctgc tcagcgacat taaccggaca gaggtcagac
    15421 taatcataca cctcatcgca gcggctggcg caaagggtta aaaaattcac attctgtaca
    15481 gcaagtgacc tgctacactg cttcaacact accactcaga aggcaactca ctatgacaga
    15541 caatacttat cagcccgcga aagtctggac gtgggataaa tccgctggcg gcgcgttcgc
    15601 caatatcaat cgcccgtttt ctggtccgac gcatgaaaaa acgctgcccg ttggcaaaca
    15661 tccattgcaa ctttattcgc tgggaacgcc gaacggtcag aaagtaacga ttatgcttga
    15721 ggagctactg gcgctgggcg ttactggtgc agagtacgac gcctggctga ttcgtattgg
    15781 cgatggcgat caattctcca gcggctttgt cgaagtgaac ccaaactcga agatcccggc
    15841 gctgcgcgat catacgcata atccgccgat ccgcgtgttt gaatctggtt cgatcctgct
    15901 ttatctggcg gagaaatttg gctacttcct gccgcaggat ctggcaaaac ggactgaaac
    15961 gttgaactgg ctgttctggt tacagggtgc cgcgccgttc ctcggcggtg gttttggtca
    16021 cttttaccat tacgcgccgg tgaaaattga gtacgccatc aaccgcttta ccatggaagc
    16081 caagcgcctg ctcgacgtgc tggataagca actggcgcag cataagtttg ttgcgggcaa
    16141 tgagtacacc attgcggata tggcgatttg gccgtggttt ggcaacgtgg tgctcggcgg
    16201 cgtgtatgat gccgcagagt ttctggatgc gggaagttat aaacatgtgc aacgctgggc
    16261 gaaagaagtg ggcgaacgtc cggcagtgaa acgtgggcgt attgttaacc gcaccaacgg
    16321 gccgctgaac gagcagttgc atgagcgcca tgacgccagt gattttgaga caaatacaga
    16381 agataagcgt caggggtaag ggttggtgtt cgtcgcagca agccatccag gccggataag
    16441 gcgttcacgc cgcatccggc aatcgtgcac aatgcctgat gcgacgctgc cgcgtcttat
    16501 caggcctaca aaagctaatc atcacgcgct ggtaatgtcg taatccattt ggcgcagtgc
    16561 gtctaatgtg gctttggctt catcttcgtc gatgatgctc atggcaaatc cgacgtgtac
    16621 cagcacccac tggcccagca gatcggcagg gttaccttcg caaatcaggg cgatattcac
    16681 gtcgcgcttg ataccacata cttcaacctg cgcaagctgg tgaatatctt caccgacagc
    16741 cagcacctgg cctggaacgc caatacacat tgttaactcc ggttatttcg attgccggac
    16801 aaacgcgaaa aagaaccttt agcgggtgtt ccctctcacc ctaaccctct ccccggaggg
    16861 gcgaggggac cgtccgtgcg ctttttaggc gttgtcctca gtcttattca acttcaatac
    16921 ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga cagagcggac
    16981 actgcgcatc gtgctgatga atctccacca cctggctgca atcccagcac caggcctggg
    17041 cgggtttata gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt
    17101 caaaactaaa acggacggcg ctctcctcaa cgcaggagag cgcgccaatt tccagccaca
    17161 cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcgc
    17221 tctggcaaag cgacaactca tgcattttcg cccctcctgc ggccaaacag cagggcgcga
    17281 cgccctgcgt gtggtacatc cggattggtg actggcaacg aaaggatcat tcgtgcgcag
    17341 tcatccgtca ggcgttggcc ttcttcaatc gacatgctgt gcgaaagcgg cgacatcagc
    17401 gaacaggaga ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt catggtgcca
    17461 tacggcagtt gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg
    17521 ccggggaaga taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct
    17581 tcgaacagcg taaaatcaga aacatacacc ggcattgaag gatgcagaaa agagagatcg
    17641 tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt cggggaggtc
    17701 tggaaacctg ctatctcttc agtcatgaat cgcctcccgt gggatggctt ccacgcctga
    17761 ttcgcgcagc gcagccagaa ccagctcaag cgcaggttca atcattgctt caaccgtcgg
    17821 cgttaagccg atgtgtggct ccagcgattc cgggatcacg ccgaccaggg tcagcttttt
    17881 cggaaactca ccggtgaagc gcagggccga caatacgtcg gccaggccaa gctgatgcgg
    17941 agagattttg ttggtaaaca acgccggaac ttcttcatcc cgcaggatca tcatcgttcc
    18001 cggcgtgttc tttttcgaca caatggcatc cgcaataatc agatgatcgc gatttgccat
    18061 gtcgccaagc agctccattc ccgccgtgcc gccatcgagg atctcaacat aatccggcag
    18121 aatgtatcgt tgctctaacg cttcgacaat ccgcacgccg atggcttcat cggtcagcaa
    18181 aatattgccg acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg
    18241 ttgccgtcgg catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg
    18301 gtacgcacca cttccagcgg tttattcgga tcggcaaccg gtgtacccac cagcgactgc
    18361 tcgtaaggac cgacgtcatc attgaagtta cgcggaccag agttccaggt tgatggaaca
    18421 accgcctggt agttgctgat gataccgtct ttaataacca tccagtgaga gagcataccg
    18481 cgcggcgctt cgaggaagcc aacacctttg aactcacccg ttgccggaat gttcggcttc
    18541 acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttggttttgc
    18601 aggatatcct gcaattcaca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag
    18661 tgcagctgtg ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca
    18721 ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt tcaccagcat attagccagc
    18781 ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga atatttcccg
    18841 tcgtcagacc aaccatcata agccggaatg gtggtacctt cccatggcgc ctgtggcgct
    18901 tcgtctttat accaggagtg cttcgcgctt tcctgaatgc ctttgatcag gtattcatcg
    18961 gaatgagaag tgatcggacg atacgaggac agatccgcat tctcaatgta gccgcccggg
    19021 aacaggaagc tgccgttttt gctgtcggtc gggaattccg gcacgctcag gtagttcacc
    19081 gcacctttac cgcgcgtcag ccattccggg tagaacgcgg cgataaccgc ggtatcaacc
    19141 ttataaacct gctcaacaaa gtcgctcagt ttgtcgatga aagacttgat gtacatcagg
    19201 cgctcaaggt tcaacacgcc caacccgtcg aggttgattg ggttcgcgac accactcact
    19261 gccaggttct gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg gttagcgtca
    19321 cgctggcact ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc
    19381 agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg accgctggca
    19441 accagatctt tgatcttgtt ctgaacttta gtgaactctt ccggactgtt cagatgccag
    19501 gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt cagcttgcag tgcagaagtg
    19561 atgtccaccc agtccagcgc cgaaagctga tagaaatgaa cgatatggtc atgcgtggtg
    19621 tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagc
    19681 gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac gccacagata
    19741 cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg
    19801 cgccacatgg taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg
    19861 atgcgtaaat gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt
    19921 tgctcgcctc atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac
    19981 gaatgagtac gatgtaagcg caaatctcaa tagccacaaa accaatagaa atcaacagtt
    20041 cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc aacgaatagg
    20101 tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac agcatgcggg
    20161 aatcattacg cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt
    20221 caatccagaa catcacggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat
    20281 agataagctc gccaaagcgc agcccgatga aaatcgccag caacacactg atggtgttgg
    20341 tcagcttgac gaacaggctc ttttcatccg gaccgttgcc acgcagaccc gcctgcacca
    20401 gtgaaccttc aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca
    20461 gcggcaacat ttcatagctc tgccagagcg gatgcacctt gtagcccgcc gagatcatca
    20521 gcgaccccat tgaagactgg tgcatggtcg gcagcagcgc accgagcgcg atgatgaaga
    20581 acatcacctt gttcagacgt tgtagcgaca ccttccagcc cagacgttca aacagtgccg
    20641 gagcaaactc cagagccatc acgccgatgt agatggtcat acagaccgcc gtctcgaaca
    20701 gtaccgagtt cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac
    20761 ccacgtcgat agtgatcgac aagccaccca gtgagtaacc aaacagactc gccaacagcg
    20821 ccggacgcac cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg
    20881 cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc cacacgcccc
    20941 acgggaagcc gccgttcagg tcagagacag agcccagacc gaacaccaga cgcttcacaa
    21001 tcaggagcat acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt
    21061 tgccgcccag cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt
    21121 ttggtgttgc gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg
    21181 cctttataca gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt
    21241 ttcggcaggt cgagattttc ataaggcaca cccgtcagta ccagaacctg agtaccgccg
    21301 ccctctttct cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg
    21361 ccagatttca gcgtctgacg cggatagtgg tattcgctgc caggcttcag cgccagacgt
    21421 tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc cgccgggcac
    21481 acttctacgc agccaggcag accgccttta tcgagacgtt ccacaccttt ctggttgcac
    21541 agctcgcact tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg cacgttgtac
    21601 ggacaggcga ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta atggacaatg
    21661 ccggttttcg gatctttttt cagtgcagag accgggcaca cagagacaca gttcggatcg
    21721 acgcagtgca tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg
    21781 acccctgtgc cgctggtcca cacctgaatg atgttattgg tatacggcga cagtttgtcg
    21841 ttgttcgacc aggtctgttc cccttgcggg ttacgttcag ggaagttgat atcctgacac
    21901 ttggtgacgc aagcctggca gcctacgcac aaggtcgagt cgtacaacat ccccagcgat
    21961 cccggaattg gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc
    22021 agcaatgccc cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc
    22081 gtgagtcagc gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga
    22141 caccggcaac cagcccaacc acaccgccga gcaaaccaat agcgcctgca gagacgttgc
    22201 cgccctcttt agcgttaaca tccggtttct gtgaacgcgg agtctgattt tcgacgttgg
    22261 caagctgatg gatgccttta tggaagccga tacctttttc gttacagcca tagcacgggt
    22321 gaccaatcgc caccggccac acaccgccaa catcgcagaa ttgcagcgtt gagcagttgc
    22381 cgtaagtttc tggcccttta cagccgaggt ggtacaggca ccagccttcg cggtggcctt
    22441 catcaccgaa ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt
    22501 cgtgaatcag acggccatag gcgaaggtcg gacggttttt gtcatccagt ttcggcggtt
    22561 tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc
    22621 ccggaatatt gataacggtt ttgcctggca gaacttcttg caagctgact gcgccagttg
    22681 ggttaactcc agctgcggca acaccgcccc acgcggagca ggaaccaata gcgataatgg
    22741 ctgctgcgcc ttccgccgct ttgcggatgt gatccacaat cggctcaccg gcaaccatgc
    22801 aataaatacc gttatctttt aatgggatgg aaccatccac cactaacaca tactgccctt
    22861 tgtacttctc gagagcgtta tgtttgttct cttcgacctg atgaccgaag gcggcggaaa
    22921 gcacttcgtg atattccaga gagatagtct ccagcacgag gttttctacc gttggatgcg
    22981 ttgcacgaag cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg
    23041 gcggacgctg cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc
    23101 ccatggtggc ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag
    23161 aatggatgag ggtgttatct ccagtcattt atagttattc cgttgcgagg acctggcata
    23221 tattttgcct caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat
    23281 tatggttatc gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag
    23341 tgtaattaat taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc
    23401 cataaaaaag cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc
    23461 tttatccagt ccatagcggc gcgtcagaat acccgcttcc caggcgctaa gctggcgatc
    23521 tccggtcttt tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt tataatcggc
    23581 acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa
    23641 gcctttacca aaatggttat tcatcgttca cccattcgtt atatatcgcc atatatagcg
    23701 atattttagg caaagaacga cttttgcgca cgctattttt tcatacttgt ttaagacgta
    23761 atcccgtaag agataaacct atcaggagag atatttatcg aaccatttca gtgtcctttg
    23821 ccaggcaaga tcggcggcag atttgtcata acggggcgtg gaatcattat ggaatccgtg
    23881 attaaccccc ggatagatat aagcctcata aaccttatta ttgtctttca acgccgcctc
    23941 gtaaggaggc cagccctcgt tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg
    24001 cgcttcaatc ttcgccacat cggcagtggg tgcctgacga ccataaaacg gcaccgcgca
    24061 ggccagttcc ggatacgcga cagccgccgc attcgatacg ccaccgccat agcaaaatcc
    24121 ggtaataccc actttacctg tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa
    24181 gaaatcattc atcagtttgg ttggatcaac ctgctgttgc agctcacgac ctttatcatc
    24241 atttcccgga taacctccaa cggaacttaa tccgtcaggt gccagggcga tataccccgc
    24301 cttcgccact cgccgtgcca catcttcgat atacggattc agtccacgat tctcatgcac
    24361 cacccccacg gctggcgttt tgccgctcat ttttgcgggt ttcaccagat aaccccgtac
    24421 ctcgccgtga ccatttggcg aaggatacgt gatgtactca gcaacaatct ccgggtcggt
    24481 aaactctacc tgagtcgcca gcgcataatt tggcttgagc aaatcaaaca acgctaatgc
    24541 cgtcatcccg cccaccgcat acttcgccgc aaggttgagg aactcacgtt tcgaaatttt
    24601 cccgtgaacg taatagtcgt agtaatccaa caactcttgt gggaaatctt tggcggttaa
    24661 acgcggcatc gttgcattcc tcaactggtg tttttttaag caaagcataa gcgcgtgttt
    24721 ttgcccagtt tttcgtcact ctgtgagcca gactacggga tacgcgctgg cgaatcgcta
    24781 aactagaaac attgtttcga aattgaacgg tggaaaggag aggtcatggt ctggttagcg
    24841 aatcccgaac gttacgggca gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg
    24901 cccgcgttat cgctcggttt atggcacaat ttcggtcacg ttaacgcgct ggaatcacag
    24961 cgtgcaatcc tgcgtaaagc gtttgattta ggcattacgc actttgattt agccaacaat
    25021 tacgggccgc ctccaggaag cgcagaagag aactttggtc gcctgctgcg cgaggatttt
    25081 gctgcttatc gcgatgaact gattatctct accaaggctg gctacgatat gtggcccggc
    25141 ccgtacggct ctggcggttc acgtaaatac ctgctcgcca gcctcgacca aagcctaaag
    25201 cgtatggggc ttgagtatgt cgatatcttt tactctcatc gcgtcgatga aaatacgccg
    25261 atggaagaaa ccgcctctgc gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc
    25321 gggatctcct cttactcgcc agagcggacg caaaaaatgg tcgagttgct gcacgagtgg
    25381 aaaattccgc ttttaattca tcaaccttcg tacaatttac tgaaccgctg ggtggataaa
    25441 agcggcctgc tggataccct gcaaaataac ggcgtgggct gtattgcctt tactcctctg
    25501 gctcagggat tgctgaccgg aaaatatctc aacggcattc cgcaagattc acggatgcat
    25561 cgtgaaggga ataaaattcg tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac
    25621 agcctgcgct tattgaatga aatggcacag cagcgtggac aatcaatggc gcaaatggcg
    25681 ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg
    25741 gagcaactag aggagaacgt gcaggcgctg aataatctga catttagcac cgaggagctg
    25801 gcgcagattg atcagcatat cgccgatggc gaactgaatc tgtggcaggc gtcgtccgat
    25861 aaatgacctg ttaataacgg gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg
    25921 agtcagtcgg tcaagatagc ccatcataaa tgcagaaagc acaaacgtca gatggataat
    25981 gacgtaccac atcagtttgt tatccgggac atttttcgca tccataaaga cgcgcagtaa
    26041 gtgaatggaa gaaattgcca caatcgacgc tgctactttg tttttcagcg acgttgcgtc
    26101 cattttcccc agccagttca gcttctcttt gttctcggag atatccagct gtgagacgaa
    26161 attctcataa ccggaaaaca tcaccatcac cagcaaaccg ccaaccagcg tcatatccac
    26221 cagcgacagc aacacgagga tcaaatctga ttccgccatc gagaagatat tcggcagtac
    26281 gtgaataatc tcctggaaga acttcagcgc cagggcaact aacgccagcg aaaggccaaa
    26341 gtacacgggg gcaagcagcc agcgagaagc atacattgca ttttcaagaa aacgttccat
    26401 acattcctgt cgtgtagtta aaacgggcaa cagtatatcg caattgagta aacagcacgc
    26461 aacagcaaat caacatatac gttaaacctt tgtcgcataa ttctgaggtt tagggatctt
    26521 cctggttccc ttatcaacgc atcgagagcg tctaccctta aagggcgttg aacctgaagg
    26581 gagaaaaacg atgtctcatt taaaagaccc gaccacgcag tattacactg gtgaatatcc
    26641 caaacagaaa caaccgacgc caggcatcca ggcgaagatg acaccgatac cggattgcgg
    26701 cgagaaaacc tatgttggta gcgctcgcct gaaagatcgt aaagcactgg tgacaggggg
    26761 cgattccgga ataggtcgcg ctgccgccat cgcttacgcg cgagaagggg ctgacgtggc
    26821 gatcagttat cttcccgtgg aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga
    26881 atgcggacgc aaagccgttc tgctgccagg cgatttaagc gatgagaaat tcgcccgttc
    26941 gctggttcac gaagcgcaca aggcgttagg cgggctggat attatggcgc tggtcgccgg
    27001 gaaacaggtt gccattccgg atattgcaga cctcaccagc gaacagtttc aaaagacctt
    27061 tgccattaac gttttcgcgc tgttctggct aacccaggaa gcgatccccc tgctaccgaa
    27121 aggtgcaagt atcatcacca cttcgtcaat ccaggcatac cagccaagtc cgcatttact
    27181 ggactatgcg gctacgaagg cggcgattct gaactacagc cgtggcctgg caaaacaggt
    27241 cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc cctatctgga cggcactgca
    27301 aatttccggc ggacaaacgc aggataagat cccgcagttt ggtcagaaaa cgccgatgaa
    27361 gcgtgcgggg caaccggcgg aactggcccc tgtatatgtt tatctggcaa gtcaggagtc
    27421 gagctacgtc accgcagaag tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa
    27481 tgcccggttg tgaaaagcaa ccgggcatca ttgtgaattc actcttacct gatgtggcga
    27541 aaacgcctta tccgcctaca aacgcatgaa gattcaacat gttgcaatag attttgtagg
    27601 cctgataagc gtagcgcatc aggcattgag cacctaatgc cggatgcgtc gtgaacgcct
    27661 tatccggcct acaaacgcac gaagattcaa catgttgcaa tagattttgt aggcctgatg
    27721 agcgtagcgt atcaggcaat tctactttac ttcgctttgg cggtttcttc gccgaccaga
    27781 cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa
    27841 tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca
    27901 ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg
    27961 tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa
    28021 gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc
    28081 agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta
    28141 tcgtcgaggt tttcgttaag atgcattgcc atcggacatt atcctgcgcg taatttttgt
    28201 gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc
    28261 aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca
    28321 aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac
    28381 agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata
    28441 ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc
    28501 gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc
    28561 cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct
    28621 gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca
    28681 aaatcagcgg cgatatcgtt ggcctggttt aaggaacgtg cttcagccag cagttgctgc
    28741 tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc tcttactgaa gaagattgcc
    28801 caggtgacta cggaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg
    28861 tgctgataca taccccagac ggaaaggtcc gtctgcatta aattattacc cactgtgtat
    28921 ctccaggacg caagtcacaa aatctgcgca taataatatc aaaacgacgt cgaattgata
    28981 gtcgttctca ttactatttg catgctgccg tacctttgct ttcttttcct tgcgtttacg
    29041 cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca
    29101 ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc
    29161 cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata
    29221 cactcttggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtat ttgagagtat
    29281 ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg
    29341 cggaacgtta acccattttt ccttacaaca agcgatgtgt gaactggaag gtggcgcagg
    29401 ctgcgcgcta tttccctgcg gggcggcagc ggttgctaat tcgattcttg cttttgtcga
    29461 acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag
    29521 caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga
    29581 tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc cgggttccat
    29641 cactatggaa gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga
    29701 tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt
    29761 tggcatcgat gtttctattc aggccgccac caaatatctg gttgggcatt cagatgcgat
    29821 gattggcact gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa atgcctatct
    29881 gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt
    29941 aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggccgaat ggctggcaga
    30001 acatccgcaa gttgcgcgag ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt
    30061 ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact
    30121 caatgatgaa gagctggcga actatctgga taacttcagt ttattcagca tggcctactc
    30181 gtggggcggg tatgaatcgt tgatcctggc aaatcaacca gaacatatcg ccgccattcg
    30241 cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga
    30301 tgtcgacgat ctgattgccg atctggacgc cggttttgca cggattgtat aacattgcca
    30361 cttttggaca attttgcaga cattttattg tgaaaagtct taaattgttg cgtccgggat
    30421 caaggcgtcc cggacgattc aggagtacaa taggcagata aaggcttaaa cgctgttcca
    30481 caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg
    30541 ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt
    30601 ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg
    30661 caggcgcatt gattgcccaa ggggttatgg attttctgcc tacgattgcg attctgaccg
    30721 ccgcagcaag tctgggctgc tggctaagtt atattcaggg gcgctggtta gggaatacca
    30781 aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca
    30841 tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta
    30901 cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca
    30961 actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta
    31021 tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa
    31081 ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact
    31141 gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc
    31201 tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac
    31261 tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgctgctgc gctggccttc
    31321 atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc
    31381 aacggcgagg tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg
    31441 gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc aattcgtttc
    31501 agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg
    31561 ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata
    31621 cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgaa taaaatctct
    31681 tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct
    31741 tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct
    31801 gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc
    31861 agcaggtatt cattggcatc ataacgaaac acgcg
//
LOCUS       DADMFL010000040        30227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.040, whole genome shotgun sequence.
ACCESSION   DADMFL010000040 DADMFL010000000
VERSION     DADMFL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.040"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..63
                     /locus_tag="JNZ27_001804"
     CDS             <1..63
                     /locus_tag="JNZ27_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8664166.1"
                     /translation="FAIREGGRTVGAGVVAKVLS"
     gene            293..676
                     /gene="secE"
                     /locus_tag="JNZ27_001805"
     CDS             293..676
                     /gene="secE"
                     /locus_tag="JNZ27_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY8664167.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            678..1223
                     /gene="nusG"
                     /locus_tag="JNZ27_001806"
     CDS             678..1223
                     /gene="nusG"
                     /locus_tag="JNZ27_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY8664168.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1382..1810
                     /gene="rplK"
                     /locus_tag="JNZ27_001807"
     CDS             1382..1810
                     /gene="rplK"
                     /locus_tag="JNZ27_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY8664169.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1814..2518
                     /gene="rplA"
                     /locus_tag="JNZ27_001808"
     CDS             1814..2518
                     /gene="rplA"
                     /locus_tag="JNZ27_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY8664170.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2931..3428
                     /gene="rplJ"
                     /locus_tag="JNZ27_001809"
     CDS             2931..3428
                     /gene="rplJ"
                     /locus_tag="JNZ27_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY8664171.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3495..3860
                     /gene="rplL"
                     /locus_tag="JNZ27_001810"
     CDS             3495..3860
                     /gene="rplL"
                     /locus_tag="JNZ27_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY8664172.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4180..8208
                     /gene="rpoB"
                     /locus_tag="JNZ27_001811"
     CDS             4180..8208
                     /gene="rpoB"
                     /locus_tag="JNZ27_001811"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY8664173.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8285..12508
                     /gene="rpoC"
                     /locus_tag="JNZ27_001812"
     CDS             8285..12508
                     /gene="rpoC"
                     /locus_tag="JNZ27_001812"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY8664174.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12750..13883)
                     /gene="thiH"
                     /locus_tag="JNZ27_001813"
     CDS             complement(12750..13883)
                     /gene="thiH"
                     /locus_tag="JNZ27_001813"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY8664175.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13880..14725)
                     /gene="thiG"
                     /locus_tag="JNZ27_001814"
     CDS             complement(13880..14725)
                     /gene="thiG"
                     /locus_tag="JNZ27_001814"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY8664176.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14652..14852)
                     /gene="thiS"
                     /locus_tag="JNZ27_001815"
     CDS             complement(14652..14852)
                     /gene="thiS"
                     /locus_tag="JNZ27_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY8664177.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14836..15591)
                     /gene="thiF"
                     /locus_tag="JNZ27_001816"
     CDS             complement(14836..15591)
                     /gene="thiF"
                     /locus_tag="JNZ27_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY8664178.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15584..16219)
                     /gene="thiE"
                     /locus_tag="JNZ27_001817"
     CDS             complement(15584..16219)
                     /gene="thiE"
                     /locus_tag="JNZ27_001817"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8664179.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16219..18114)
                     /gene="thiC"
                     /locus_tag="JNZ27_001818"
     CDS             complement(16219..18114)
                     /gene="thiC"
                     /locus_tag="JNZ27_001818"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY8664180.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWYLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGSQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18195..18295)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18348..18824)
                     /gene="rsd"
                     /locus_tag="JNZ27_001819"
     CDS             complement(18348..18824)
                     /gene="rsd"
                     /locus_tag="JNZ27_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY8664181.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18919..19692
                     /gene="nudC"
                     /locus_tag="JNZ27_001820"
     CDS             18919..19692
                     /gene="nudC"
                     /locus_tag="JNZ27_001820"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY8664182.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19732..20796
                     /gene="hemE"
                     /locus_tag="JNZ27_001821"
     CDS             19732..20796
                     /gene="hemE"
                     /locus_tag="JNZ27_001821"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY8664183.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20953..21624
                     /gene="nfi"
                     /locus_tag="JNZ27_001822"
     CDS             20953..21624
                     /gene="nfi"
                     /locus_tag="JNZ27_001822"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY8664184.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21667..22257
                     /locus_tag="JNZ27_001823"
     CDS             21667..22257
                     /locus_tag="JNZ27_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY8664185.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22444..22716
                     /gene="hupA"
                     /locus_tag="JNZ27_001824"
     CDS             22444..22716
                     /gene="hupA"
                     /locus_tag="JNZ27_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY8664186.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22729..23424
                     /locus_tag="JNZ27_001825"
     CDS             22729..23424
                     /locus_tag="JNZ27_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY8664187.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQDAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLYNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="JNZ27_001826"
     CDS             complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="JNZ27_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY8664188.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24089..25465
                     /gene="zraS"
                     /locus_tag="JNZ27_001827"
     CDS             24089..25465
                     /gene="zraS"
                     /locus_tag="JNZ27_001827"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY8664189.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQRGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25462..26787
                     /gene="zraR"
                     /locus_tag="JNZ27_001828"
     CDS             25462..26787
                     /gene="zraR"
                     /locus_tag="JNZ27_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY8664190.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26784..28073)
                     /gene="purD"
                     /locus_tag="JNZ27_001829"
     CDS             complement(26784..28073)
                     /gene="purD"
                     /locus_tag="JNZ27_001829"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY8664191.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28085..29674)
                     /gene="purH"
                     /locus_tag="JNZ27_001830"
     CDS             complement(28085..29674)
                     /gene="purH"
                     /locus_tag="JNZ27_001830"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY8664192.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7093 a         7873 c         8310 g         6951 t
ORIGIN      
        1 ttcgcaatcc gtgaaggcgg ccgtaccgtt ggcgcgggcg ttgtagcaaa agttctgagc
       61 taattgccga taacatttga cgcaatgcgc actaaaaggg catcatttga tgcccttttt
      121 gcacgctttc gtaccagaac ctggctcatc agtgattttc tttgtcataa tcattgctga
      181 gacaggctct gttgagggcg tataatccga aaagctaata cgcgtttcga tttggtttgc
      241 ctcgcgatcg cggggtgaaa atgtttgtag aaaacttctg acaggttggt ttatgagtgc
      301 gaataccgaa gctcaaggaa gcgggcgcgg cctggaagcg atgaagtggg tcgttgtggt
      361 ggcattgctc ctggcggcga ttgtcggcaa ctacctttat cgcgacatta tgctgccgct
      421 gcgtgcgctg gccgtagtaa ttctgattgc tgcagcgggt ggtgtcgcgc tgttaacgac
      481 aaaaggtaaa gctaccgttg cttttgcccg tgaagcgcgt accgaagtcc gtaaggtcat
      541 ttggccgact cgccaggaaa cattgcacac cacgctgatt gtggctgcgg ttaccgcagt
      601 aatgtcactg atcctgtggg gactggatgg tattctggtt cgcctggtat cctttatcac
      661 tggcctgagg ttctgagatg tctgaagctc ctaaaaagcg ctggtacgtc gttcaggcgt
      721 tttccggttt tgaaggccgc gtagcaacgt cgctgcgtga gcatatcaaa ttacacaaca
      781 tggaagattt gtttggtgaa gtcatggtac caaccgaaga agtggttgaa atccgtggcg
      841 gtcagcgtcg caaaagcgaa cgtaaattct tccctggcta cgtcctcgtt cagatggtga
      901 tgaacgacgc gagctggcac ttggtgcgca gcgtaccgcg tgtgatgggc ttcatcggcg
      961 gtacttccga tcgtcctgcg ccaatcagcg ataaagaagt cgatgcgatt atgaaccgcc
     1021 tgcagcaggt tggtgataag ccgcgtccga aaacgctgtt tgaaccgggt gaaatggtcc
     1081 gtgttaatga tggtccgttc gctgacttca acggtgttgt tgaagaagtg gattacgaga
     1141 aatctcgtct gaaagtgtct gtttctatct tcggtcgtgc gaccccggta gagctggact
     1201 tcagccaggt tgaaaaagcc taacccagcg atcaaaaaag cggcgattta atcgttgcac
     1261 aaggcgtgag attggaatac aatttcgcgc cttttgtttt tatgggcctt gcccgtaaaa
     1321 cgatttttta tatcacgggg agcctctcag aggcgttatt acccaacttg aggaatttat
     1381 aatggctaag aaagtacaag cctatgtcaa gctgcaggtt gcagctggta tggctaaccc
     1441 gagtccgcca gtaggtccgg ctctgggtca gcagggcgta aacatcatgg aattctgcaa
     1501 agcgttcaac gcaaaaactg attccatcga aaaaggtctg ccgattccgg tagtaatcac
     1561 cgtttacgct gaccgttctt tcactttcgt taccaagacc ccgccggcag cagttctgct
     1621 gaaaaaagcg gctggtatca agtctggttc cggtaagccg aacaaagaca aagtgggtaa
     1681 aatttcccgc gctcagctgc aggaaatcgc gcagaccaaa gctgccgaca tgactggtgc
     1741 cgacattgaa gcgatgactc gctccatcga aggtactgca cgttccatgg gcctggtagt
     1801 ggaggactaa gaaatggcta aactgaccaa gcgcatgcgt gttatccgcg agaaagttga
     1861 tgcaaccaaa cagtacgaca tcaacgaagc tatcgcactg ctgaaagagc tggcgactgc
     1921 taaattcgta gaaagcgtgg acgtagctgt taacctcggc atcgacgctc gtaaatctga
     1981 ccagaacgta cgtggtgcaa ctgtactgcc gcacggtact ggccgttccg ttcgcgtagc
     2041 cgtatttacc caaggtgcaa acgctgaagc tgctaaagct gcaggcgcag aactggtagg
     2101 tatggaagat ctggctgacc agatcaagaa aggcgaaatg aactttgacg ttgttattgc
     2161 ttctccggat gcaatgcgcg ttgttggcca gctgggccag gttctgggtc cgcgcggcct
     2221 gatgccaaac ccgaaagtgg gtactgtaac accgaacgtt gctgaagcgg ttaaaaacgc
     2281 taaagctggc caggttcgtt accgtaacga caaaaacggc atcatccaca ccaccatcgg
     2341 taaagtggac tttgacgctg acaaactgaa agaaaacctg gaagctctgc tggttgcgct
     2401 gaaaaaagca aaaccgactc aggcgaaagg cgtgtacatc aagaaagtta gcatctccac
     2461 caccatgggt gcaggtgttg cagttgacca ggctggcctg agcgcttctg taaactaatg
     2521 cctttacgtg ggcggtgatt ttgtctacaa tcttaccccc acgtataatg cttaatgcag
     2581 acgtatatcc gagatattcg ggttgtggca aggcggcaac tgagtgagtc gccaggagca
     2641 tagctaacta tgtgactggt gcgaatgaag gaagccaacg ccgccacaag ttgaatagcg
     2701 acggatagaa aagatttgtt cgttggagcc tggcctatcc aggcctccgt cgaagaccgc
     2761 aggagtttcg caagaaactt aatcccctgc gtagacggtg acagaacgct aagattattc
     2821 ttttatattc tggcttgttt ctgctcaccg taattaagac gctctctccg tttggaggag
     2881 tgaagtgagt tccagagatt ttctctggca aacatccagg agcaaagcta atggctttaa
     2941 atcttcaaga caaacaagcg attgttgctg aagtcagcga agtagccaaa ggcgcgctgt
     3001 ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa aatgactgaa ctgcgtaaag
     3061 caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa caccctgctg cgccgtgctg
     3121 ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt tggtccgacc ctgattgcat
     3181 actctatgga acacccgggc gctgctgctc gtctgttcaa agagttcgcg aaagcgaatg
     3241 caaaatttga ggtcaaagcc gctgcctttg aaggtgagct gatcccggcg tctcagatcg
     3301 accgcctggc aactctgccg acctacgaag aagcaattgc acgcctgatg gcaaccatga
     3361 aagaagcttc ggctggcaaa ctggttcgta ctctggctgc tgtacgcgat gcgaaagaag
     3421 ctgcttaatc gcagttatct ttttaacgca ttcgcttacg tataaactta ttctgatatt
     3481 caggaacaat ttaaatgtct atcactaaag atcaaatcat tgaagcagtt gcagctatgt
     3541 ctgtaatgga cgttgtagaa ctgatctctg caatggaaga aaaattcggt gtttccgctg
     3601 ctgctgctgt agctgtagct gctggcccgg ttgaagctgc tgaagaaaaa actgaattcg
     3661 acgtaattct gaaagctgct ggcgctaaca aagttgctgt tatcaaagca gtacgtggcg
     3721 caactggcct gggtctgaaa gaagctaaag acctggtaga atctgcaccg gctgctctga
     3781 aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa agctctggaa gaagctggcg
     3841 ctgaagttga agttaaataa gccaaccctt ccggttgcag cctgagaaat caggctgatg
     3901 gctggtgact ttttagtcac cagccttttt gcgctgtaag gcgccagtag cgtttcacac
     3961 tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc gacgacttaa tatactgcga
     4021 caggacgtcc gttctgtgta aatcgcaatg aaatggttta agcgtgatag caacaggcat
     4081 tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt gcacaaactg tccgctcaat
     4141 ggacagatgg gtcgacttgt cagcgagctg aggaacccta tggtttactc ctataccgag
     4201 aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag ttctggatgt accttatctc
     4261 ctttctatcc agcttgactc gtttcagaaa tttatcgagc aagatcctga agggcagtat
     4321 ggtctggaag ctgctttccg ttccgtattc ccgattcaga gctacagcgg taattccgag
     4381 ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg acgtccagga atgtcaaatc
     4441 cgtggcgtga cctattccgc accgctgcgc gttaaactgc gtctggtgat ctatgagcgc
     4501 gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag aagtctacat gggcgaaatt
     4561 ccgctcatga cagacaacgg tacctttgtt atcaacggta ctgagcgtgt tatcgtttcc
     4621 cagctgcacc gtagtccggg cgtcttcttt gactccgaca aaggtaaaac ccactcttcg
     4681 ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg gttcctggct ggacttcgaa
     4741 ttcgatccga aggacaacct gttcgtacgt atcgaccgtc gccgtaaact gcctgcgacc
     4801 atcattctgc gcgccctgaa ctacaccaca gagcagatcc tcgacctgtt ctttgaaaaa
     4861 gttatctttg aaatccgtga taacaagctg cagatggaac tggtgccgga acgcctgcgt
     4921 ggtgaaaccg catcttttga catcgaagct aacggtaaag tgtacgtaga aaaaggccgc
     4981 cgtatcactg cgcgccacat tcgccagctg gaaaaagacg acgtcaaact gatcgaagtc
     5041 ccggttgagt acatcgcagg taaagtggtt gctaaagact atattgatga gtctaccggc
     5101 gagctgatct gcgcagcgaa catggagctg agcctggatc tgctggctaa gctgagccag
     5161 tctggtcaca agcgtatcga aacgctgttc accaacgatc tggatcacgg cccatatatc
     5221 tctgaaacct tacgtgtcga cccaactaac gaccgtctga gcgcactggt agaaatctac
     5281 cgcatgatgc gccctggcga gccgccgact cgtgaagcag ctgaaagcct gttcgagaac
     5341 ctgttcttct ccgaagaccg ttatgacttg tctgcggttg gtcgtatgaa gttcaaccgt
     5401 tctctgctgc gcgaagaaat cgaaggttcc ggtattctga gcaaagacga catcattgat
     5461 gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg aagtcgatga tatcgaccac
     5521 ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg aaaaccagtt ccgcgttggc
     5581 ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc tgggcgatct ggataccctg
     5641 atgcctcagg atatgatcaa cgccaagccg atttccgcag cagtgaaaga gttcttcggt
     5701 tccagccagc tgtctcagtt tatggaccag aacaacccgc tgtctgagat tacgcacaaa
     5761 cgtcgtatct ccgcactcgg cccaggcggt ctgacccgtg aacgtgcagg cttcgaagtt
     5821 cgagacgtac acccgactca ctacggtcgc gtatgtccaa tcgaaacccc tgaaggtccg
     5881 aacatcggtc tgatcaactc tctgtccgtg tacgcacaga ctaacgaata cggcttcctt
     5941 gagactccgt atcgtaaagt gaccgacggt gttgtaactg acgaaattca ctacctgtct
     6001 gctatcgaag aaggcaacta cgttatcgcc caggcgaact ccaacctgga tgaaggaggc
     6061 cacttcgtag aagacctggt aacttgccgt agcaaaggcg aatccagctt gttcagccgc
     6121 gaccaggttg actacatgga cgtatccacc cagcaggtgg tatccgtcgg tgcgtccctg
     6181 atcccgttcc tggaacacga tgacgccaac cgtgcattga tgggtgcgaa catgcaacgt
     6241 caggccgttc cgactctgcg cgctgataag ccgctggttg gtactggtat ggaacgtgct
     6301 gttgccgttg actccggtgt aactgcggta gctaaacgtg gtggtgtcgt tcagtacgtg
     6361 gatgcttccc gtatcgttat caaagttaac gaagacgaga tgtatccggg tgaagcaggt
     6421 atcgacatct acaacctgac caaatacacc cgttctaacc agaacacctg tatcaaccag
     6481 atgccgtgtg tgtctctggg tgaaccggtt gaacgtggcg acgtgctggc agacggtccg
     6541 tccaccgacc tcggtgaact ggcgcttggt cagaacatgc gcgtagcgtt catgccgtgg
     6601 aatggttaca acttcgaaga ctccatcctc gtatccgagc gtgttgttca ggaagaccgt
     6661 ttcaccacca tccacattca ggaactggcg tgtgtgtccc gtgacaccaa gctgggtccg
     6721 gaagagatca ccgctgacat cccgaacgtg ggtgaagctg cgctctccaa actggatgaa
     6781 tccggtatcg tttacattgg tgcggaagtg accggtggcg acattctggt tggtaaggta
     6841 acgccgaaag gtgaaactca gctgacccca gaagaaaaac tgctgcgtgc gatcttcggt
     6901 gagaaagcct ctgacgttaa agactcttct ctgcgcgtac caaacggtgt atccggtacg
     6961 gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa aagacaaacg tgcgctggaa
     7021 atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt ctgaagaact gcagatcctc
     7081 gaagcgggtc tgttcagccg tatccgtgct gtgctggtag ccggtggcgt tgaagctgag
     7141 aagctcgaca aactgccgcg cgatcgctgg ctggagctgg gcctgacaga cgaagagaaa
     7201 caaaatcagc tggaacagct ggctgagcag tatgacgaac tgaaacacga gttcgagaag
     7261 aaactcgaag cgaaacgccg aaaaatcacc cagggcgacg atctggcacc gggcgtgctg
     7321 aagattgtta aggtatatct ggcggttaaa cgccgtatcc agcctggtga caagatggca
     7381 ggtcgtcacg gtaacaaggg tgtaatttct aagatcaacc cgatcgaaga tatgccttac
     7441 gatgaaaacg gtacgccggt agacatcgta ctgaacccgc tgggcgtacc gtctcgtatg
     7501 aacatcggtc agatcctcga aacccacctg ggtatggctg cgaaaggtat cggcgacaag
     7561 atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac tgcgcgaatt catccagcgt
     7621 gcgtacgatc tgggcgctga cgttcgtcag aaagttgacc tgagtacctt cagcgatgaa
     7681 gaagttatgc gtctggctga aaacctgcgc aaaggtatgc caatcgcaac gccggtgttc
     7741 gacggtgcga aagaagcaga aattaaagag ctgctgaaac ttggcgacct gccgacttcc
     7801 ggtcagatcc gcctgtacga tggtcgcact ggtgaacagt tcgagcgtcc ggtaaccgtt
     7861 ggttacatgt acatgctgaa actgaaccac ctggtcgacg acaagatgca cgcgcgttcc
     7921 accggttctt acagcctggt tactcagcag ccgctgggtg gtaaggcaca gttcggtggt
     7981 cagcgtttcg gggagatgga agtgtgggcg ctggaagcat acggcgcagc atacaccctg
     8041 caggaaatgc tcactgttaa gtctgatgac gtgaacggtc gtaccaagat gtataaaaac
     8101 atcgtggacg gcaaccatca gatggagccg ggcatgccag aatccttcaa cgtattgttg
     8161 aaagagattc gttcgctggg tatcaacatc gaactggaag acgagtaatt ctcgctcaaa
     8221 caggtcactg ctgtcggggt aaaacccggc agcggattgt gctaactccg acgggagcaa
     8281 atccgtgaaa gatttattaa agtttctgaa agcgcagact aaaaccgaag agtttgatgc
     8341 gatcaaaatt gctctggctt cgccagacat gatccgttca tggtctttcg gtgaagttaa
     8401 aaagccggaa accatcaact accgtacgtt caaaccagaa cgtgacggcc ttttctgcgc
     8461 ccgtatcttt gggccggtaa aagattacga gtgcctgtgc ggtaagtaca agcgcctgaa
     8521 acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg acccagacta aagtacgccg
     8581 tgagcgtatg ggccacatcg aactggcttc cccgactgcg cacatctggt tcctgaaatc
     8641 gctgccgtcc cgtatcggtc tgctgctcga tatgccgctg cgcgatatcg aacgcgtact
     8701 gtactttgaa tcctatgtgg ttatcgaagg cggtatgacc aacctggaac gtcagcagat
     8761 cctgactgaa gagcagtatc tggacgcgct ggaagagttc ggtgacgaat tcgacgcgaa
     8821 gatgggtgcg gaagcaatcc aggctctgct gaagagcatg gatctggagc aagagtgcga
     8881 acagctgcgt gaagagctga acgaaaccaa ctccgaaact aagcgtaaaa agctgaccaa
     8941 gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac aaaccagagt ggatgatcct
     9001 gaccgttctg ccggtactgc cgccagatct gcgtccgctg gttccgctgg atggtggtcg
     9061 tttcgcgact tctgacctga acgatctgta tcgtcgcgtc attaaccgta acaaccgtct
     9121 gaaacgtctg ctggatctgg ctgcgccgga catcatcgta cgtaacgaaa aacgtatgct
     9181 gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc ggtcgtgcga tcaccggttc
     9241 taacaagcgt cctctgaaat ctttggccga catgatcaaa ggtaaacagg gtcgtttccg
     9301 tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt tctgtaatca ccgtaggtcc
     9361 atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg gcgctggagc tgttcaaacc
     9421 gttcatctac ggcaagctgg aactgcgtgg tcttgctacc accattaaag ctgcgaagaa
     9481 aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg gacgaagtta tccgcgaaca
     9541 cccggtactg ctgaaccgtg caccgactct gcaccgtctg ggtatccagg catttgaacc
     9601 ggtactgatc gaaggtaaag ctatccagct gcacccgctg gtttgtgcgg catataacgc
     9661 cgacttcgat ggtgaccaga tggctgttca cgtaccgctg acgctggaag cccagctgga
     9721 agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc ccggcgaacg gcgaaccaat
     9781 catcgttccg tctcaggacg ttgtactggg tctgtactac atgacccgtg actgtgttaa
     9841 cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa gcagaacgtc tgtatcgctc
     9901 tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc accgagtatg aaaaagatgc
     9961 taacggtgaa ttagtagcga aaaccagcct gaaagacacg actgttggcc gtgccattct
    10021 gtggatgatt gtaccgaaag gtctgcctta ctccatcgtc aaccaggcgc tgggtaaaaa
    10081 agcaatctcc aaaatgctga acacctgcta ccgcattctc ggtctgaaac cgaccgttat
    10141 ttttgcggac cagatcatgt acaccggctt cgcctatgca gcgcgttctg gtgcatctgt
    10201 tggcatcgat gacatggtca tcccggagaa gaaacacgaa atcatctccg aggcagaagc
    10261 agaagttgct gaaattcagg agcagttcca gtctggtctg gtaactgcgg gcgaacgcta
    10321 caacaaagtt atcgatatct gggctgcggc gaacgatcgt gtatccaaag cgatgatgga
    10381 taacctgcaa actgaaaccg ttattaaccg tgacggtcag gaagagaagc aggtttcctt
    10441 caacagcatc tacatgatgg ccgactccgg tgcgcgtggt tctgcggcac agattcgtca
    10501 gcttgctggt atgcgtggtc tgatggcaaa gccggatggc tccatcatcg aaacgccaat
    10561 caccgcgaac ttccgtgaag gtctgaacgt actccagtac ttcatctcca cccacggtgc
    10621 tcgtaaaggt ctggcggata ccgcactgaa aactgcgaac tccggttacc tgactcgtcg
    10681 tctggttgac gtggcgcagg acctggtggt taccgaagac gattgtggta cccatgaagg
    10741 tatcatgatg actccggtta tcgagggtgg tgacgttaaa gagccgctgc gcgatcgcgt
    10801 actgggtcgt gtaactgctg aagacgttct gaagccgggt actgctgata tcctcgttcc
    10861 gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg gaagagaact ctgtcgacgc
    10921 ggtaaaagta cgttctgttg tatcttgtga caccgacttt ggtgtatgtg cgcactgcta
    10981 cggtcgtgac ctggcgcgtg gccacatcat caacaaaggt gaagcaatcg gtgttatcgc
    11041 ggcacagtcc atcggtgaac cgggtacaca gctgaccatg cgtacgttcc acatcggtgg
    11101 tgcggcatct cgtgcggctg ctgaatccag catccaggtg aaaaacaaag gtagcatcaa
    11161 gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa ctggttatca cttcccgtaa
    11221 caccgaactg aaactgatcg acgaattcgg tcgtaccaaa gaaagctaca aagtgcctta
    11281 cggtgcggta ctggcgaaag gcgatggcga acaggttgct ggcggcgaaa ccgttgcaaa
    11341 ctgggacccg cacaccatgc cggttatcac cgaagtaagc ggttttgtac gctttactga
    11401 catgatcgac ggccagacca ttactcgtca gaccgacgaa ctgaccggtc tgtcttcgct
    11461 ggtggttctg gattccgcag aacgtaccgc aggtggtaaa gatctgcgtc cggcactgaa
    11521 aatcgttgat gctcagggta acgacgttct gatcccaggt accgatatgc ctgcgcagta
    11581 cttcctgccg ggtaaagcga ttgttcagct ggaagatggc gtacagatca gctctggtga
    11641 caccttggcg cgtattccgc aggaatccgg cggtaccaag gacatcaccg gtggtctgcc
    11701 gcgcgttgcg gacctgttcg aagctcgtcg tccgaaagaa ccggcaatcc tggctgaaat
    11761 cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa cgtcgtctgg ttatcacccc
    11821 ggtagacggt agcgatccgt acgaagagat gattccgaaa tggcgtcagc tcaacgtgtt
    11881 cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac ggtccggaag cgccgcacga
    11941 cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac atcgttaacg aagtacagga
    12001 cgtataccgt ctgcagggcg taaagattaa cgataaacac atcgaagtta tcgttcgtca
    12061 gatgctgcgt aaagctacca tcgttaacgc gggcagctcc gacttcctgg aaggcgaaca
    12121 ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg gaagcgaacg gcaaagtggg
    12181 tgcaacttac tcccgcgatc tgctgggtat caccaaagcg tctctggcaa ccgagtcctt
    12241 catctccgcg gcatcgttcc aggagaccac tcgcgtgctg accgaagcag ccgttgcggg
    12301 caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc gtgggtcgtc tgatcccggc
    12361 aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc cgtgctgcgg gtgaagctcc
    12421 ggctgcaccg caggtgactg cagaagacgc atctgccagc ctggcagaac tgctgaacgc
    12481 aggtctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
    12541 tcggcgggtt tttttatggg tgttattcag agggcaaatt cttcacggaa aatattaaga
    12601 aaggcagggc gaaacgagga agaagctttt cgccctggac aacattcctg ctgacggcac
    12661 taccataaaa aaacacaacg ctttcaacaa ccttgcgttt ttcatcttcc gtagcggctc
    12721 ggaataaatt tacgttctta cattccgtct catagtcttt gcgaggcgcg tcccagatag
    12781 ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg ttaacgcggc agcaaccgct
    12841 tccggtctgc gatcgtcgtg cggtgagaac tgttccaact cggggtgatt atcggcatag
    12901 ccacctggct gcgttttcga gaaggcgctg acgttattga tcgccagcgg aataacgcga
    12961 tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt caatctccgg tgcaagcagt
    13021 cggaaggcgc agatggtttg cactaactgg cgttcatcca taatcgacgc aggctcaatg
    13081 ccgccagtac acgggcgcag gcgcggaaag gagacagagt aacggctttg ccagtaatgc
    13141 tgttgcagcc atagcaaatg ttcggcaacc atatagcagt caacgcgcca gttgtcggaa
    13201 aggccaatta gcgcgccgag gcctatctta tcaatccccg cacgccccag ccgatccggc
    13261 gtttccagcc gccagaagaa gtcctgtttt ttgcctttca aatgatggcg ggtataagtc
    13321 gcctcgtgat atgtctcctg ataaaccatc accccatcca gaccaagttg ctttaactcg
    13381 gcgtattccg tctccgccag cggttgcact tccatttgta gtgaagagaa ctgttcacgc
    13441 agggcgggga gatgacgacg aaagtaatcc atccccactt tcgcctgatg ttcaccagtg
    13501 actaatagca gatgttcaaa gcccatctcc cgtatagcgg cgctttccct ggcaatatcc
    13561 gcttcatcca gcgttttgcg cttgatgcga ttactcatgg aaaatccgca gtacgtgcag
    13621 ttgttagcgc aaagattgga aagataaagc gggacgtaga aactaactgt gttgccaaat
    13681 cgctgacggg tcagacgctg cgcctgttgg gccagttgtt ccagatagcc actggcggca
    13741 ggcgataaca gcgccatcat gtcgtcgcgg gtgagttgcg aggcatttag cgcccgctct
    13801 acgtcagcag ccgttttgcc gttgatacgc aggcggatgt cgtcccagtc cagttgtcgc
    13861 cagcgattgc tgaaggtttt catgccgatg cctccagaaa tccggtcagc gggctggtgg
    13921 catgagcaaa ataactgcgg ctgcccggtc cggactgacg tgccagcagg cctgcttcta
    13981 ccgccagacg aaatgccttc gccatgttga cgggatcgtc cgcgacggca atcgccgtat
    14041 tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc ggcatggctg ggaacgccga
    14101 tgccagcatc gacaaccacc ggaactgtgg cctgctggat aataatctcc agcatggcac
    14161 gggtttccag tccctgattc gagccaatcg gcgcgccgag cggcatcacc gctgcacagc
    14221 cgacttcttc cagacgttta cacaataccg gatcggcccc gcagtaaggc agcacgacaa
    14281 atccctgttg taccagcgtt tcggcggctt tcagggtttc gatgggatcg ggcaacagcc
    14341 agcgggcgtc agggtgaatc tccaatttta gccagtttgt gcctaacgct tcacgtgcca
    14401 gatgggcggc gaaaatggct tcttccgctg ttttcgcccc ggaagtattc ggcagcaggg
    14461 ccacacccgc cgcgataagc ggttcgagga tagcgtcgtt gtgctggcgc aagtcgacac
    14521 gtttcatcgc cagtgtcacc agctggctgc cggaagcgcg gatcgcctcc accatcagtt
    14581 gtgaagaagc gaatttccct gtgccggtaa acagatgtga atcaaacgtt ttgtccacaa
    14641 tacgtaacat ctcaaccccc tgcaataacc tgaaaaagca ggatctggtc gccatcctgc
    14701 acgatatgtt gcgtccactg ctcacgcggg acgatttgct gattaatcgc cagagccgcg
    14761 cccgcttgtc gttggtcgag ttgctccagt agttcgtgaa cagtttgccc ggcggcgcac
    14821 tgcatcggtt gatcgttaaa caggatctgc attgcttcct ccgcataccg ggcaaccact
    14881 ggcgcggcgc aacgccaggc tgcgccactg gctcgactta ccgtcgaaca gtcggagttc
    14941 tcccgcaggc gtctctatac cgcttaataa cttaatggct tccagtgcct gcaaagtgcc
    15001 catcaccccg accaccgggc caaccacgcc cgccgtgcgg cagttgcgtt ctggctgctg
    15061 gttatctggc cacaggcagc ggtaacaccc ctgctcccag ggcggtgtca gtaccatcaa
    15121 ctgaccgcca aatccgaccg cgctggcggt gataagcggc gtgttgagtg ccacgcaggc
    15181 ggcattaatc tcctggcgag tcgccatatt gtcggtacag tcgagcacca catcggcttg
    15241 tgcaaccgca tctttcaacg cctcacccgt taaccgttgt tgtaatgctg tcagttgaat
    15301 gtcgggattc aactgtgtca gtcgctgttg gctgacctgc gatttcgggc tgttgatatc
    15361 ttcagtggta aagaggattt gtcgttgcag attgcttaaa tgcacatcgt cgtcatctgc
    15421 cagtaccagc gtcccgacgc cagcccctgc caggtatagc gcagcaggtg tacccagccc
    15481 gccaagacca ataatcagca cctggctgtc gagcagtttt tgctgcccgt caagagcgat
    15541 atcgtcgagc agaatttggc ggctataacg cataaagtca cggtcattca tcgccaactc
    15601 ctgcaatttc cagcaactgt gccgttgcca aacgccagtc tgcggcttga gtaatggcgc
    15661 tgacgacggc gatactgccg acacccgttg ctatcaccgc aggcgcgcgt gccagactga
    15721 taccgccaat cgccacggtg ggataatccg ccaggcgctc aacatgccgt gccaactgtt
    15781 ccagcccctg cggtgcagaa ggcatctgtt tggtttgcgt cgggaacaca tgtcccagcg
    15841 cgatataaga ggggcgtgct gccagcgcga cgtcgatttc catatcgtca tgtgtcgaaa
    15901 cgcccagccg caggcctgcc gcgcggatgg cattgagatc ggtggcttgc aaatcttcct
    15961 gccccaaatg gacgccatac gcctgatgct tgatcgcgag ccgccagtaa tcgttgataa
    16021 acaatcgcgc gttatagcgg cggcccagcg caattgccgc cacgacatcg gcttccacct
    16081 cttcatcgcg ccgatctttg atgcgtagct ggagagtacg tacgcctgca tccaacagac
    16141 gttcgatcca ctgtacgctg tccaccaccg ggtacagtcc taaacgaaaa ggtacaggag
    16201 gaaaatcagg ctgatacatc acgcttcctc cttatgcagg tagatttctc cgcctctggc
    16261 acggaagttc tccgacatat ccgccattcc cacttcaatg gtttgcgcgg cggcgtaatc
    16321 gcgtacttcc tggctgattt tcatcgagca gaattttggc ccacacatgg agcaaaaatg
    16381 ggcgactttg ccggactctt ggggcagggt ttcatcgtga taagcgcggg cggtaaacgg
    16441 gtcgagggcc agattaaact ggtcttccca gcgaaattcg aagcgggctt tcgacatggc
    16501 gttatcgcga atttgcgagc ccggatgccc ttttgccagg tcagcagcgt gggcggcaat
    16561 cttataagtg ataagcccct gcttaacatc ttctttattg ggcagaccca gatgctcttt
    16621 tggcgttacg taacagagca tcgcgcagcc aaaccagcca atcatcgccg caccaatccc
    16681 cgacgtgaag tggtcatagc ccggtgcaat atcggtagtt agcggcccca gagtgtaaaa
    16741 cggcgcttcg tggcagtgct ctaactcctc ggtcatattg cggcggatca tctgcatcgg
    16801 cacatggcct gggccttcaa tcatcacctg tacgtcatat tcccaggcga ttttggtcag
    16861 ttcgcccagc gtatgcaatt cggcaaactg cgcttcatcg ttggcgtcct gaatagaacc
    16921 ggggcgcaga ccgtcgccca gcgacagcga aacgtcataa gcggcacaga tttcacaaat
    16981 ttcgcggaag tgttgataga ggaaattttc ctgatgatgg gagaggtacc atttcgccat
    17041 aatcgaaccg ccgcgagaga cgataccggt caggcgtttc gcggtcatcg gcacatagcg
    17101 cagcagtacg cccgcatgga tagtgaagta atccacacct tgctcggcct gttccagcag
    17161 cgtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc ccgttaacct tctccagcgc
    17221 ctggtagatc ggcactgtac cgatcggcac cggactgtta cgcaaaatcc actcgcgggt
    17281 ttcgtgaata tagcgaccgg tggagagatc catcaccgta tccgctcccc agcgcgtgga
    17341 ccataccagc ttttccactt cttcttcgat ggaagaggtg accgccgagt tgccgatatt
    17401 ggcgttaact tttaccagga agttacggcc aataatcatc ggctccgatt ccggatgatt
    17461 aatgttggcc gggataatcg cacgtccggc agcaacttca tcacggacaa attccgcagt
    17521 gatattttcc ggcagatgtg cgccaaagct cattcccgga tgctggtggc gtaaaacctc
    17581 gctacggatg cgctcgcggc ccatattctc gcggatggcg atgaattcca tttccggcgt
    17641 gatgatgccc tggcgggcgt agtgcagttg ggtgacacgg cgtcctgttt tggcgcgttt
    17701 tggcgttaat aagccgctaa aacgtagctc gtcgagacca tcatctgcca gccgcgttct
    17761 ggtgtaatcg gaactgcgca cggtaagttc ttcggtatcg gcgcgcgcat cgatccacgg
    17821 ctggcgtagt tttgccagcc cttgctgcac gttaatggcg atctgcggat caccatacgg
    17881 gccggaggtg tcgtagaccg gaatcgcttc gttttcttcg tactgcggct gttctttgct
    17941 accgccaatt agcgtcgggc taagctggat ctcacgcatc ggcacgcgca cgccggggtg
    18001 tgtgccagtg atataaatgc gttttgagtt gggaaaggcg gtgccttcca gggtgtcgat
    18061 aaaatgttgg gcccgggcgc gttgttcgcg gcgggtcagt tttgttgcag acatagctca
    18121 ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg aagcaagaga cgatcgccgc
    18181 aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc aggtattagc ctgatcaggt
    18241 tccgcggatc ccgaataaac ggtctcagcc agttaaggca ctccgacaag aaattagccc
    18301 ccacaaacgg ggcgttgaat gtaaattacg cgttaacagc gcagaactca agcaggatgt
    18361 ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac aaagcgcgct
    18421 tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt atcatgatcg
    18481 atggcggttt ccaggctgga atcgtagtaa tccataatct gttgggtatt ggcttcgagt
    18541 tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt atgaagaatg
    18601 cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg acaaaaatca
    18661 tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat gccaaccaga
    18721 ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac cagtttgtta
    18781 cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga ctccgcaagt
    18841 ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg ccagtgatgc
    18901 aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg ctggtgggtc
    18961 gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg cgaagcggca
    19021 aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg ggaacctgtt
    19081 tggttaatac aacagcagcg gcgtcacgat atggggtcgg tacgtcaggt cattgatctc
    19141 gatgttgggc tgtttcaact ggccggacgt ggcgtgcaac tggcggagtt ttaccgatcg
    19201 cataaatact gtggttactg cgggcatgaa atgtatccga gcaaaaccga atgggcgatg
    19261 ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat tattgttgcc
    19321 atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa cggtgtccat
    19381 acagtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcagt cgcgcgggaa
    19441 gtgatggaag agagcggaat taaagttaaa aacttgcgtt acgtgacttc tcagccgtgg
    19501 ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg cgacatcgtg
    19561 atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt gccgttactc
    19621 ccgccgcccg gcaccgtagc gcgccgtctg atagaagata ctgtggcgat gtgtcgggca
    19681 gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa aatgaccgaa
    19741 cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt cactccagta
    19801 tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg cgcccaggcg
    19861 ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gcgaagtgac tttgcaaccg
    19921 ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac cgtgccggac
    19981 gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac ctcgccagtc
    20041 acctgcaaag ccgacgtcga taaactgcca attccggacc cggaagatga gctgggttac
    20101 gtgatgaacg cggtgcgtac cattcgtcac gaactgaaag gcgaagtgcc gctgattggt
    20161 ttttccggca gcccgtggac gctggcaacc tacatggtgg aaggcggcag cagcaaagcc
    20221 ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc tctgctcgat
    20281 aaactggcga aaagcgtcac tttgtatctg aatgcgcaga ttaaagccgg tgctcaggca
    20341 gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca acagttctcg
    20401 ctctattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg tcgccgcgtg
    20461 ccagtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc tgaaaccggt
    20521 tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg ccgtgtgggc
    20581 aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc gcctgcccgc
    20641 attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg tcatgtcttt
    20701 aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt gttcgtggag
    20761 gcagtgcatc gactgtctga acagtatcac cgctaagagc ctgatccatc aggctatttt
    20821 atttgccatt ttgaggccgg gcagtgctca aaatcctcac gtactacgtg tacgctccgg
    20881 tttttccgcg ctgtccggcc tcaaactgcc ttcaacaatt acgcctgttg aaccaggctc
    20941 taaggagcga ttatggatct cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct
    21001 gtgatctgcg aggatcgact cgataaagat ccaccggatc tgatcgccgg agccgatgtc
    21061 gggtttgagc agggcggaga agtgacgcga gcggcgatgg tgctgctgaa atatccctcg
    21121 cttgagctgg tggagtataa agttgcccgc atcgccacca ccatgcctta cattccaggt
    21181 tttctttcct tccgcgaata tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag
    21241 ccggatttag tgtttgtcga tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc
    21301 agccattttg gcttattggt ggatgtgccg accattggcg tggcgaaaaa acggctctgc
    21361 ggtaaattcg aaccgctctc cagcaaaccg ggcgcgctgg ccccactgat ggataaaggc
    21421 gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc
    21481 ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat
    21541 cgtctgccgg agccaacgcg ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg
    21601 cgctatacag caaatcagcc ctaattcagg taaactgcgg ataatttccg tatttgagaa
    21661 ctcaacatgt tacaaaaccc aattcatctg cgtctggagc gcctggaaag ctggcagcat
    21721 gtcactttca tggcttgctt atgcgaacgc atgtacccca attacgccat gttctgccag
    21781 caaaccggtt ttggtgatgg gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg
    21841 ctgaccgtta aagacgcaaa agtaaatttc gacagccaac tggagaaatt tgaagaagcg
    21901 attccttcag ccgacgattt cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg
    21961 gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa
    22021 gtgagtaaga cctccatcac gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa
    22081 atgagcgatg aagagcttaa agaaaaccca gctgtagagc aagaatggga cattcagtgg
    22141 gaaatattcc gacttttagc tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt
    22201 agggcagacc tgcgtgagtc gggtgagagc aatattggta taatttttca gcaataagac
    22261 cagaaaacgt gatttaacgc ctgatttgtc gtacctggag tcttcccttt cgccccccgt
    22321 ctggtctaca tttggggggc gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc
    22381 tattctggca tttccgtcgc actcgatgct tagcaagcga taaacacatt gtaaggataa
    22441 cttatgaaca agactcaact gattgatgta attgcagaga aagcagaact gtccaaaacc
    22501 caggctaaag ctgctctgga gtccactctg gctgcaatta ctgagtctct gaaagaaggc
    22561 gatgctgtac aactggttgg tttcggtacc ttcaaagtga accatcgcgc tgagcgtact
    22621 ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg cagctaacgt accggcattt
    22681 gtttctggca aggcactgaa agacgcagtt aagtaagatt gcgtggcagt gaacagtttt
    22741 aacgaagggg tggtttcacc ccttttgtct ttctggcgtc gatcattgat gctggcaggt
    22801 gcgctgtttc tcactgcctg tagccacaac tcttcacttc ctccctttac cgccagtgga
    22861 tttgctgaag accaggacgc ggtacgcatc tggcgaaaag acagcggcga taatgtgcat
    22921 ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg
    22981 cagggcgata acctcacgct catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt
    23041 cgcgcgcgtt ttgacgatcg cggcgatctg agctttatgc aacgtgaatc cgatggggaa
    23101 aagcagcagc tttataacga ccaaatcgat ttataccgtt atcgtgctga tcagatccgc
    23161 cagattagcg atgccttacg tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg
    23221 atggaacaga ccgtgaccac ctgcgaaggg caaaccatta aacctgattt agattcgcag
    23281 gcgatagcgc atatcgagcg ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg
    23341 ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa actctgattt ctgtcgctgg
    23401 caacccaacg agaaaacgtt ctgatttacc agtggcccat acccatatga ccgccaccac
    23461 cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg aatacccgct tcagccatcg
    23521 cgatatctcg tttcacccgt aactcatcta acgactgacg caaattctcc atctctttgg
    23581 cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc taacagggca ttgtattcat
    23641 aacgcttcgt caccagttgc tgttgcagtg cgctgctttg agcgtaaaag tcattatgga
    23701 ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac
    23761 cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc cattgctgaa agcgccatca
    23821 ttaccagggc aattttcgtg ttccgtttca tggttaatcc tccagtggtt gtcttacttc
    23881 gggtattgca tcttccgtgc caacgatgaa acgctgatat gacgggtaat ctggcatgat
    23941 aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc atttttactc attgaaactt
    24001 aagcctttgt gttacagcgc agggtaagcg ctgataaaag atggcatgat ttctgctgtc
    24061 agaaagggat gagcaggcaa agaagaagat gcgttttatg caacgttcta aagactcctt
    24121 agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt gggctggtag ggttgtttgc
    24181 ggtgacggtg attcgcgatt atgggcgcga gactgccgcc gccagacaaa cgctgctgga
    24241 aaaaggcagt gtacttattc gcgctcttga atccggctcg cgcgtcggca tggggatgcg
    24301 catgcatcat gcgcagcagc aggccttact ggaagaaatg gccgggcagc ctggagtacg
    24361 ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg catagcaact ccggcatggt
    24421 gggaaaacag ctttattccc ctcaggaaat gcagcagtta catccgggag atgaagaagc
    24481 gtggcggcgg atcgatagcg cagacggcga gcctgttctg gaaatttatc gccagtttca
    24541 accgatgttt gctgctggaa tgcaccggat gcgccatatg cagcagtatg ccgcgacacc
    24601 acaagcaatt ttcatcgctt tcgacgccag taatattgtg agtgccgaag atcgtgagca
    24661 gagaaacacc ctgattattc tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc
    24721 attcttctgg tatcgccgct atctgcgctc gcgccagctt ctacaagatg aaatgaagcg
    24781 caaagagaag ctggtggcgc tagggcatct tgcggcaggc gttgcccacg aaatccgtaa
    24841 cccactttcc tcgattaaag gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg
    24901 agaagcgcat caactggcgc aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt
    24961 aagcgagttg ctggaactgg ttaagccaac gcatctggct ttgcaggcgg tggatctcaa
    25021 cacgctgatt aaccactcat tacagctggt aagccaggat gcaaacagcc gggagatcca
    25081 gttacgcttt accgccaacg acacattacc ggaaattcag gccgatccgg acaggctgac
    25141 tcaggtcctg ttgaatctct atctcaatgc tattcaggcg attggtcagc gtggcgtgat
    25201 tagcgtgacg gccagcgaaa gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa
    25261 gggaattgcg gcagatcagc ttgaagccat cttcactccg tacttcacca ccaaagccga
    25321 aggcaccgga ttggggctgg cggtcgtgca taatattgtt gaacaacacg gtggtacaat
    25381 tcaggtcgca agccaggagg gaaaaggctc aacgttcacc ctctggcttc cggtcaatat
    25441 tacgcgtaag gacccacaag gatgacgcac gataatatcg atattctggt ggtggatgat
    25501 gacattagcc actgcactat tttgcaggct ttactgcgcg gctggggcta taacgtcgcg
    25561 ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg aacaggtttt tgatcttgtg
    25621 ctttgcgatg tgcgaatggc ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg
    25681 ttaaacccgg caattccggt gctgattatg actgcgtact ccagcgtcga gacggcggta
    25741 gaggcactga aaactggggc gctggattat ctcatcaagc cgctggattt cgataaccta
    25801 caggcgacgc tggaaaaggc gctcgcgcat acgcacagta ttgatgctga aacacctgcg
    25861 gtgactgcca gccagttcgg tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt
    25921 gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga tccacggcga ttccggcacc
    25981 ggtaaagagc tggtcgccag ggcgattcac gccagtagcg cacgtagcga aaaaccgctg
    26041 gtaacgctca actgtgcggc actcaacgaa tccttgctgg aatctgaatt gttcggtcac
    26101 gaaaaagggg cgtttactgg agccgataaa cgccgggaag ggcgctttgt tgaggcggac
    26161 ggcggcacgc tgtttctcga tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg
    26221 ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta gcaaccagac tatctcggtt
    26281 gatgtccggc tgattgcggc gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt
    26341 tttcgccagg atctctacta tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg
    26401 caacggcggg aagatatccc tctgctggct ggccattttc ttcagcgctt tgccgagcgt
    26461 aatcgcaagg cggtaaaagg ctttacgcca caggcgatgg atttgctgat ccactacgac
    26521 tggccgggaa atattcgtga actggaaaac gcggtggaac gggcggtggt gctgctgacc
    26581 ggggaatata tttccgaacg cgagctgccg ctggcgattg ccagtacgcc gatcccgctg
    26641 gggcaaagtc aggatattca gccgctggtg gaagtggaaa aagaggtgat tctggcggcg
    26701 ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa
    26761 acgctattgg caaaactgtc gcgttagttc tgctcgcgtt cgatggcgcg ccaaccgata
    26821 tctttccggc agaagcaatc atcccagtgg atatcggtca ttaaggcgta ggcgcgtttc
    26881 tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca gtacgcgccc gccgctggtt
    26941 accacctgct cgtcatccgc cagttttgtg cccgcgtgga acactttgcc gtcagccact
    27001 tcttccagcg gcaggccgtg gatcacatcg ccggtgcggt aatcgcccgg atatccaccc
    27061 gcagccatca ccacgctgag agaagcacgt tcatcccact ctgacgtttt ctcgtccagt
    27121 ttgccttcac aggccgccag gcagagctca accagatcgg acttcatgcg cagcataatc
    27181 ggctgggttt ccggatcgcc aaagcggcag ttaaattcaa taaccttcgg attgccctgt
    27241 ttgtcgatca tcaggcccgc atagagaaaa ccggtgtagg tgttgccttc cgccgccatg
    27301 cctttcacgg ttggccagat gatacgttcc atggtgcgct gatgaacttc atcggttact
    27361 accggcgcgg gggagtaagc gcccatcccg ccggtgtttg gtccgttatc tttatcgcct
    27421 acgcgtttgt gatcctggct ggtagccatc ggcagcacat gctcgccgtc caccatcacg
    27481 ataaagctcg cttcttcgcc atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg
    27541 ccaaaagcgt tgcccgccag catatcgtga acagccgctt ccgcttcttc cagcgtcatc
    27601 gccacgataa cgcctttccc ggcagccaga ccgtccgctt taatgacgat tggcgcgcct
    27661 ttctcacgca gatacaccag cgcaggttct acctcggtga agttctggta ttccgccgta
    27721 gggatcttat ggcgggccag gaaatctttg gtaaacgctt ttgagccttc cagttgggcc
    27781 gcacctgcgg ttgggccgaa gattttcagc ccggcggcgc ggaaggtatc gaccacgcct
    27841 ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa
    27901 tccaacagcg ccgggatatc ggtcacgccg atagcaacgt tttgcagcgc gggttccagc
    27961 gcagtgcctg cattacccgg agcaacaaaa acagtctcaa ccagcggcga ctgggccgct
    28021 ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca ctaatacttt catctattgc
    28081 tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg
    28141 cggcggcaat cacttcgtca tcacggatgg aaccgccagg ctggattacg caggtcacgc
    28201 ccgcagcggc ggcggcatca ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg
    28261 aggaaccttt cacttccagg ccttcatcgg cagctttaat accggcgatt ttcgcggagt
    28321 acacgcggct catctggccc gcgccaatac cgatggtcat attgtttttg gcatagacga
    28381 tagcattgga tttcacgaat ttcgccactt tccagcagaa cagcgcatca cgcagttcct
    28441 gttcggtcgg ctgacgttgg gtgacgacgc gcagttcttc cgcaccgacc atccccaggt
    28501 cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct
    28561 cgccccactg accgcaggtc agaacgcgta cgttctgctt ggcggcggtg attttcaggg
    28621 cttcttcgct ggcggaaggc gcaataatca cttcgacaaa ctgacgagaa atgatggcct
    28681 gtgcggtttc cgcatccagc tcgcggttaa aggcaatgat gccgccgaat gcggaggttg
    28741 ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga attgccgata gctacaccgc
    28801 aagggttggc gtgcttcaca atcacacatg ccggctcggc gaactctttc acgcactcca
    28861 gcgccgcatc ggtatcggcg atgttgttat aagagagggc tttaccctga acctgggttg
    28921 cggtagcaac ggaggcttct ttcacattct cttctatata gaaggcagcc tgctggtggc
    28981 tgttctcgcc gtaacgcata tcctgcttct taatgaagtt caggttcagc gtgcgtggga
    29041 agcgaccggc ggcttctttg ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt
    29101 tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac
    29161 gggttgcaag cgtcagcgat ccttcgttgt catccatctc tttaataatg gcgtcgtagt
    29221 cgctgctctt caccacgatt gcgacatctt tatggttctt agcggcggag cgcaccatgg
    29281 tcgggccgcc gatatcgatg ttctcaaccg catcttccag cgagcagcct tcacgagcaa
    29341 cagtctgggc gaacggatac aggttaacaa ccaccatatc gataggctgg atctgatgtt
    29401 cttccataat ggcatcgtcc tggccgcgac ggcccagaat gccaccatgt actttcggat
    29461 gcagggtctt cacgcgtcca tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg
    29521 ttaccggcag acctttttct gctaacagac gggcagtgcc ccctgtagac agcagctcca
    29581 caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc ggctttgtca gaaacactga
    29641 gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa tcccctgggt ttgactatta
    29701 cagagagcgt tagctgaatt tttcgcgaaa aactcagcta acgcccctaa cggggcatcc
    29761 ttattttttc gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt
    29821 tcaatggtga tcacaatttt gacagtggtt accgtgggaa aaatacagaa attacattga
    29881 tgattgtgga taactctgtg cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag
    29941 cagtcagtca tttttctgca atttttctat tgcggcctgc ggagaactcc ctataatgcg
    30001 ccttcatcga cacggcggat gtgaatcact tcacacaaac agccggttcg gttgaagaga
    30061 aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg
    30121 acagtgagct gaaagccgcg tcgcaactgc tctttaacaa tttatcagac aatctgtgtg
    30181 ggcactcgaa gatacggatt cttaacgtcg caagacgaaa aatgaat
//
LOCUS       DADMFL010000041        29734 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.041, whole genome shotgun sequence.
ACCESSION   DADMFL010000041 DADMFL010000000
VERSION     DADMFL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.041"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            347..640
                     /locus_tag="JNZ27_001831"
     CDS             347..640
                     /locus_tag="JNZ27_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664193.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JNZ27_001832"
     CDS             712..1371
                     /locus_tag="JNZ27_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY8664194.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JNZ27_001833"
     CDS             1448..1909
                     /locus_tag="JNZ27_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY8664195.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNZ27_001834"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNZ27_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JNZ27_001835"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JNZ27_001835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY8664196.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQYPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /locus_tag="JNZ27_001836"
     CDS             3808..5076
                     /locus_tag="JNZ27_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY8664197.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSTSVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNZ27_001837"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JNZ27_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY8664198.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNZ27_001838"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JNZ27_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY8664199.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLILTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="JNZ27_001839"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="JNZ27_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY8664200.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="JNZ27_001840"
     CDS             complement(8331..9863)
                     /locus_tag="JNZ27_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY8664201.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /locus_tag="JNZ27_001841"
     CDS             10193..11491
                     /locus_tag="JNZ27_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY8664202.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="JNZ27_001842"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="JNZ27_001842"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY8664203.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14165)
                     /locus_tag="JNZ27_001843"
                     /pseudo
     CDS             complement(12739..14165)
                     /locus_tag="JNZ27_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14775..16511)
                     /locus_tag="JNZ27_001844"
     CDS             complement(14775..16511)
                     /locus_tag="JNZ27_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY8664204.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNZ27_001845"
     CDS             complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="JNZ27_001845"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY8664205.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17620..18231
                     /gene="emtA"
                     /locus_tag="JNZ27_001846"
     CDS             17620..18231
                     /gene="emtA"
                     /locus_tag="JNZ27_001846"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY8664206.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18233..18967)
                     /locus_tag="JNZ27_001847"
     CDS             complement(18233..18967)
                     /locus_tag="JNZ27_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY8664207.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19168..19422
                     /locus_tag="JNZ27_001848"
     CDS             19168..19422
                     /locus_tag="JNZ27_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY8664208.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19472..20932)
                     /locus_tag="JNZ27_001849"
     CDS             complement(19472..20932)
                     /locus_tag="JNZ27_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY8664209.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNWVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20869..21426)
                     /locus_tag="JNZ27_001850"
     CDS             complement(20869..21426)
                     /locus_tag="JNZ27_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HAY8664210.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21452..22306)
                     /locus_tag="JNZ27_001851"
     CDS             complement(21452..22306)
                     /locus_tag="JNZ27_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY8664211.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22306..23115)
                     /locus_tag="JNZ27_001852"
     CDS             complement(22306..23115)
                     /locus_tag="JNZ27_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY8664212.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23125..23883)
                     /locus_tag="JNZ27_001853"
     CDS             complement(23125..23883)
                     /locus_tag="JNZ27_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8664213.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23880..24860)
                     /locus_tag="JNZ27_001854"
     CDS             complement(23880..24860)
                     /locus_tag="JNZ27_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY8664214.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24860..25882)
                     /locus_tag="JNZ27_001855"
     CDS             complement(24860..25882)
                     /locus_tag="JNZ27_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY8664215.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26948..27388
                     /locus_tag="JNZ27_001856"
     CDS             26948..27388
                     /locus_tag="JNZ27_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664216.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27467..29164)
                     /locus_tag="JNZ27_001857"
     CDS             complement(27467..29164)
                     /locus_tag="JNZ27_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY8664217.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29484..>29734)
                     /locus_tag="JNZ27_001858"
     CDS             complement(29484..>29734)
                     /locus_tag="JNZ27_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8664218.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7457 a         7222 c         7638 g         7417 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aataatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc tttgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagtatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcac gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaatg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca gcaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgtt
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtat gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacccctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgtttttg
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa acccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agcggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgcccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttaatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctatt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg cgtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctagaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggta ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct
    12541 ggcgttacgc gtcccggttg tgacagtgta acttgttgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attattgact cggagtcgac
    12781 ttagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tacagttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattta gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag gcgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag ttccaccgac aggaagagcc gccctggcgg gcgtcgtacg
    14101 tctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta
    14161 gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt
    14221 ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact
    14281 ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag
    14341 ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa
    14401 aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt
    14461 gtgtgggcta tgtcagtgcg gcaggttgcg tggggaatat gttcaggcga tgtcaggcag
    14521 gggcccctga tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa
    14581 aaagccctta cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa
    14641 tggtttgtca tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg
    14701 aaatgacaag tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag
    14761 atgctgcatt aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac
    14821 acttcattat cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca
    14881 ccgacaaccg gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta
    14941 tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg
    15001 ctggcttcga gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg
    15061 ctgaacagtt taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg
    15121 ccttcgcgca gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct
    15181 gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta
    15241 tcagcactca attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca
    15301 acgcgtgaca ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac
    15361 gagagtgatg ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag
    15421 aacagtcgcg cattctccag ccccgccatc atcgggaaca cggccaggat gattggcacc
    15481 gcgccgcgta atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa
    15541 gggagcaatc cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa
    15601 atgagcgccg gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc
    15661 aggaacatgg cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag
    15721 cggttgcgaa tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg
    15781 ctgccttcca gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat
    15841 ggatataatc cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg
    15901 ccaagaccga tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa
    15961 ctgacactgc tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc
    16021 attggatcat tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc
    16081 ttaccaccca gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt
    16141 aataagcctt caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca
    16201 gaggtgataa gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag
    16261 gaactggcct gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt
    16321 accatgtagg cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc
    16381 agcatgccga tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa
    16441 ctaagtaata tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt
    16501 gtggcatcca acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat
    16561 tagcggcaga gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac
    16621 aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg ttatttagaa tggcatgtgc
    16681 gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg
    16741 aatcgacagg cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata
    16801 atcattgggc gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat
    16861 tcccgcatga tagagctgca acagcatacg ttcgacgcgg aaagggtgct cgttaatatc
    16921 ttccagcacc agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat
    16981 cagcatcgca agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc
    17041 ttgccattca atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa
    17101 ggcgttcagt tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac
    17161 attgccttgc gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag
    17221 taacgggtca tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg
    17281 actggcaccg taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc
    17341 aagggaattg agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg
    17401 ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg
    17461 cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat
    17521 ggcaattcct tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg
    17581 tcacctcaac ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc
    17641 tttttgattg tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg
    17701 aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt
    17761 gcagcctggg gcgtcgatcc gcaattgatc acggcgatta tcgctatcga atcgggtggt
    17821 aatccgaacg cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc
    17881 tccggacgtg atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg
    17941 aaaaatccag agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc
    18001 ccgctggcag gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct
    18061 aacggggcag gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa
    18121 atcaacgatt tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg
    18181 caggctccgc gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc
    18241 gcactttgtc cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt
    18301 gccgctccac cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg
    18361 tttcattctt gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt
    18421 gaaaaacacc ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta
    18481 attcggcagg ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca
    18541 agcggaaacg taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga
    18601 gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta
    18661 cggtaataaa tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact
    18721 cgactttcgc accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga
    18781 tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta
    18841 ttttgctgat cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca
    18901 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
    18961 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac
    19021 aaaacttaat acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac
    19081 gcccagcatc gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga
    19141 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
    19201 atagccggca ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
    19261 acctgtattc tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
    19321 attggcggct ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct
    19381 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
    19441 agcgatacag atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga
    19501 aattgcgacc ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat
    19561 tgaccgacgc attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
    19621 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
    19681 cttccgacag cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg
    19741 tctgcgttgg aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca
    19801 gagcgtagct caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca
    19861 gaccgctgta atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt
    19921 gcgagaggat ggcatcactc acccagttat agtaaacgct gacctcaaat ccccagtcgc
    19981 gcgtgaaggc accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg
    20041 ggttaacaat cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg
    20101 tcggaaagcg tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt
    20161 atttccccat cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc
    20221 tgccatcttt ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg
    20281 ccacgacatc gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac
    20341 gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt
    20401 tccagttaac ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc
    20461 cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag
    20521 ccagcgagtt gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca
    20581 gggtaaaacg gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact
    20641 ctggccactg ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt
    20701 tttcaccatc ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac
    20761 cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc
    20821 ctgcaatatc attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac
    20881 cgctgacttg caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg
    20941 cattgtcctg actacggctc catccctggc gatatcccag acttgcttct tcagggtggt
    21001 gatattaatg gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga
    21061 aacttcgact gcccccagat tgttggtcag aacccgcgcc agatcgaggt tgccgtcata
    21121 gggaacataa atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg
    21181 aacttttacc tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac
    21241 acttaatgcc tgagcgacat tctgtttatc caaggctttc atggtgggtt gatcgagaac
    21301 ggtggtcgtc gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc
    21361 cgctacggcc tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag
    21421 tctcatagaa taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga
    21481 ctttaatgtc agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc
    21541 agtcgaggta atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt
    21601 gaggagccag cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt
    21661 cgagctgaag tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg
    21721 cttcaacaac aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc
    21781 agccctgatt gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac
    21841 atccggcacg atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg
    21901 tgcccggaat tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca
    21961 atgccagcat ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga
    22021 ctgccttcca tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac
    22081 ccgcgttcgc ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta
    22141 acatcttaca cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta
    22201 tatagccagg ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat
    22261 cttccagcag taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg
    22321 cggtacgcta tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc
    22381 atcttgttgt tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag
    22441 actccagctg acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg
    22501 tccacggata aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa
    22561 gatatagttg ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac
    22621 gaacgaggtg ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt
    22681 gctcattgca tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca
    22741 gcgtggcagg tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc
    22801 cttcagtgcg gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc
    22861 ataaaccgta gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat
    22921 atcgaataag gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct
    22981 gtccgttggc gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg
    23041 cgttttttct gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat
    23101 atcatcaatg agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg
    23161 gtcggcagaa acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa
    23221 gccttgtgtt actcgtccat ctggctctac ctggataatg ctgtcggcaa tggcgttggc
    23281 atggagcgga tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag
    23341 cgtgtccaga agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa
    23401 caacaagcgc ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc
    23461 gctaagcgta ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc
    23521 cgtggctttc aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag
    23581 gcccatcaac accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac
    23641 ccaggcaata gcctgtgcgc gctggcgatg gctgagtgtc tgcacaggga ggctatccag
    23701 taggatctcg ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt
    23761 gccgcaacca ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac
    23821 gtcgcgtagc actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt
    23881 cactggcgac ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc
    23941 agaatgccaa gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg
    24001 agcagaatag cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc
    24061 aacaagcggg caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct
    24121 accgtgctgg cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg
    24181 ttcagtccca gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg
    24241 agaaaaaaca ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt
    24301 aaggtcacgg tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa
    24361 ggatcggcca gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg
    24421 cctaccagca ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag
    24481 acgcccgcga ccaccatcag ctcgccgcaa aatgcgtaca gctggatgta aacaatgggt
    24541 aatccccaca ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata
    24601 tccggtgaaa ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc
    24661 gctgcgccca gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt
    24721 tgttcctgca cagacacatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg
    24781 agatgataag ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata
    24841 catccgttta cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg
    24901 aacaatgcca gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg
    24961 cgggatccag ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg
    25021 catcgagcca gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt
    25081 tcacgccctg ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca
    25141 gaataatatc cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc
    25201 gatcggctat ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg
    25261 aaccctgtaa gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag
    25321 cggcgggtga ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt
    25381 gttctgcggc gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg
    25441 attgcgccag ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc
    25501 gtgcctggga gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta
    25561 acgccataag cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg
    25621 gtaactggcg aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg
    25681 aaaaaccgac cattttttct ggcgcgacag ccagcaatag caaatcggcg ggcgcgcctg
    25741 cgctgacgat gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa
    25801 ctgtttgtga aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa
    25861 acgtccgtct ggttatgggc atggtgaaca tcctgaaaag caaaatcgat atatactttc
    25921 atatatatcg ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg
    25981 tgacgacttc cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc
    26041 taatattatt taaatcattt aaggctaaat catttaaata tgaatgacat tattgttttt
    26101 tatcgtccac ggttacgcga cgttgaagtt acttgtttta caatgagcga tttttgatat
    26161 ctcgccgaga aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga
    26221 ggtgaaaaga atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct
    26281 tgctgatagt aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa
    26341 cgacgttatt aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt
    26401 actgaatgcg tttattcgaa cgtgcaaaaa aagagatgat aacagttacg gaaatatgat
    26461 atttacgctt tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa
    26521 cggcggagag ttgcagttat taagcacagg gtgtagcaat gataacattc tccattttat
    26581 taatgcattt ctcattcggg tgaaatttcc gctacctgga cgcataaaac cggaactctc
    26641 gccaaaagcc gggcactccg aaggaattga cgcgttgcgc tgctctgtaa cggcatttac
    26701 cgcaagccat ttatttcgcc agcgacaatc cgcagagaac caaacgcatc aggaaacgac
    26761 tgaacacaat aaatactgta ttgatcaaca tattatactg gcacctccag cacgcagtga
    26821 ggatacactg tatcatcagc gaatcttatc actgcggagc tggttgaacg actttctgag
    26881 cgaaataagt cggttcccgg taacaacaac agaatccttt tttaattatt gtttcgttgt
    26941 ttttggtgtg attcagaaaa taaatatttt agacagtagc cagtcaggcg ttaaaaatgg
    27001 ggcggatagt gaggcggttt tggctgcgct tattgaacat ttagaattga tcaatccttc
    27061 tggcagatta agccaaaata cccgttcggc aatgcttcag ttacgcgaag aggaatggtc
    27121 tgaattcttt ttttggttgc taaattctct ggagtgtctg gattatgtga ttattaattt
    27181 aactccagaa agcaaaaaaa cgttgatgag tgaacaccgt aacaatattc aggtagcaat
    27241 tgatgcttta tatagccaac gtcggcgtaa atcgccgggt gatgaaagtg aaacacttac
    27301 ccgtagaaat gatgctatct tcggcaacca cgtatggcaa acatttgcgc aatacttccc
    27361 gccaggactt gaaaaaccgt ctgtgtagaa aaaaccatag atatggtgtt atctgccgac
    27421 aacgccatcc ggagaagccg aatgacgttg tcggagtaag cgctggttaa ggtgtgggtt
    27481 gtgcctcttt ggttgagggt tgcgtcgttg ctgacttaac ggtcggacgc gtcgccggaa
    27541 cattgtcaca cggttgctct ttcgggcaga tcaaatccag cattttcagc gtcacgccat
    27601 tggtccagcc aaagccatcc tgtaatggat attcgccacc gccgcccccc gttccggtgg
    27661 tgctgacatc atatttttcc accagctttt tctcccggtc ataggtgtgc tgaacattgg
    27721 tcaggaagtg ccagctaatg tccatcgcca cctctttttg cccgtagttt tgtaatcctt
    27781 ctgtcgcgac ccactgtaac ggtgcccagc catttggcgc atcccattgt tgcccacttt
    27841 tcaccgacgt ggtgttcagg ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca
    27901 ttttgttggc gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg
    27961 ttaactgatt gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat
    28021 cgttccacag gtatttttcg atcccttttt gacgggcatt cgccagcgtt tcgtactggt
    28081 ttgccatcgc gttatctcct gcagctttgc tggcgcgggc gaggattttt tccattttaa
    28141 acatcaggct gttcagatcg accggtacga tgctggtggt gcgtaaggta tttaactgct
    28201 gcgggttgtc catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt
    28261 cgcggtaaat ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca
    28321 cccatgactc tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat
    28381 cctgaagttt tacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca
    28441 tccagtaagc atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt
    28501 catgctgcgc cagtaactct accatcaggg caaagaaggg cggttgcgag cggcttaaat
    28561 agtaactgcg gttgccgttg ggaatatgac cgtaagtgtc tatttcatga gcaaaattgg
    28621 ccaccatatc cgcgacttta tcccagtgac cgctttcggc aagtcctaac atggtgaagt
    28681 aactgtccca gtaatatacc tcgcgaaaac gtccgcccgg cacgacataa ggttccggca
    28741 gcggtaacag agaatcccat ttttcggtgt tttcggtaga acgcgttaat accggccaaa
    28801 gtccgtcaat atgttcgcgc agtgactgcc cctctggcgg aacatatttc tcgccttctt
    28861 tcggcagggt gaaattgacg ttaacgaaat ggcgcagatc aaatccgctc tggttttgct
    28921 gcatccgata atcagcaagg atcatcagcg gatcgctgtt cggcacggca tcggcaaagg
    28981 ttttttggtc cggaaaaagt ttggcgtttt gcacatcatt aaacagcggc cctaataaaa
    29041 tatcaggcgg ctgtggtgtt accggtgttt cttctgcctg caccgatagc gcagcgaaac
    29101 acaaaaagat acaggctgga attaacgcca ttttttgcgg gcgagaaggt gcgggggatt
    29161 tcatcaatca ttctcctttg gcgaaaccga ataaaacggt attcaagtat cagaaaacct
    29221 tagttcagga tcgcactagc tgcatgatcg aaatctcatt ctgcgaacgg tcaggcattt
    29281 tagaaaaggg aattaaaggg ataaataacg gcgaaataga gtgatcaaat aacgtctgtt
    29341 tattagccag ccagcgacat ttggttcgcg attagcgaag aaaaatcatc ataaattggt
    29401 aaaaaatata acaggtagtt aaaaaacatc agtgctgggt aaattgccgg atgacatcag
    29461 aacgatgcca tccgaacggt ggcttaaccc tgacggttga aacgttgcgt tttaacgtcc
    29521 agcgttagcg tttcttctgg ttgtatcgca tacagtttct caccctgctg gcaaatccag
    29581 ccaatcccca gttcacgggc gattagggcg ctgtgggata ccggacttcc ggcgctaagg
    29641 cagatacctt ttacaaccgc cggatccagt tgcagtactg tggaaggata aatgttctcc
    29701 gccagtagaa tagttggcga gttaaactgc ggga
//
LOCUS       DADMFL010000042        29417 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.042, whole genome shotgun sequence.
ACCESSION   DADMFL010000042 DADMFL010000000
VERSION     DADMFL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.042"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..109)
                     /locus_tag="JNZ27_001859"
     CDS             complement(<1..109)
                     /locus_tag="JNZ27_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY8664219.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1167)
                     /locus_tag="JNZ27_001860"
                     /pseudo
     CDS             complement(179..1167)
                     /locus_tag="JNZ27_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
     gene            1964..2467
                     /locus_tag="JNZ27_001861"
     CDS             1964..2467
                     /locus_tag="JNZ27_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY8664220.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2547..2798)
                     /locus_tag="JNZ27_001862"
     CDS             complement(2547..2798)
                     /locus_tag="JNZ27_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY8664221.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2913..2999)
                     /gene="azuC"
                     /locus_tag="JNZ27_001863"
     CDS             complement(2913..2999)
                     /gene="azuC"
                     /locus_tag="JNZ27_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY8664222.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3262..3585
                     /locus_tag="JNZ27_001864"
     CDS             3262..3585
                     /locus_tag="JNZ27_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664223.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3756..4253
                     /gene="ftnA"
                     /locus_tag="JNZ27_001865"
     CDS             3756..4253
                     /gene="ftnA"
                     /locus_tag="JNZ27_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY8664224.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4290..4529)
                     /locus_tag="JNZ27_001866"
     CDS             complement(4290..4529)
                     /locus_tag="JNZ27_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY8664225.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4719..5930
                     /gene="tyrP"
                     /locus_tag="JNZ27_001867"
     CDS             4719..5930
                     /gene="tyrP"
                     /locus_tag="JNZ27_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY8664226.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5992..6657)
                     /gene="yecA"
                     /locus_tag="JNZ27_001868"
     CDS             complement(5992..6657)
                     /gene="yecA"
                     /locus_tag="JNZ27_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY8664227.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6853..6939)
                     /locus_tag="JNZ27_001869"
     tRNA            complement(6853..6939)
                     /locus_tag="JNZ27_001869"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6903..6905),aa:Leu,seq:taa)
     gene            complement(6952..7025)
                     /locus_tag="JNZ27_001870"
     tRNA            complement(6952..7025)
                     /locus_tag="JNZ27_001870"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6991..6993),aa:Cys,seq:gca)
     gene            complement(7307..7855)
                     /gene="pgsA"
                     /locus_tag="JNZ27_001871"
     CDS             complement(7307..7855)
                     /gene="pgsA"
                     /locus_tag="JNZ27_001871"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY8664228.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AALTLWSMLQYLSAARADLLDQ"
     gene            complement(7912..9744)
                     /gene="uvrC"
                     /locus_tag="JNZ27_001872"
     CDS             complement(7912..9744)
                     /gene="uvrC"
                     /locus_tag="JNZ27_001872"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY8664229.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRMAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9741..10397)
                     /gene="uvrY"
                     /locus_tag="JNZ27_001873"
     CDS             complement(9741..10397)
                     /gene="uvrY"
                     /locus_tag="JNZ27_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY8664230.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10693..10869
                     /locus_tag="JNZ27_001874"
     CDS             10693..10869
                     /locus_tag="JNZ27_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664231.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10856..11080
                     /locus_tag="JNZ27_001875"
     CDS             10856..11080
                     /locus_tag="JNZ27_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY8664232.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11113..11733)
                     /gene="sdiA"
                     /locus_tag="JNZ27_001876"
                     /pseudo
     CDS             complement(11113..11733)
                     /gene="sdiA"
                     /locus_tag="JNZ27_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11963..12715)
                     /gene="yecC"
                     /locus_tag="JNZ27_001877"
     CDS             complement(11963..12715)
                     /gene="yecC"
                     /locus_tag="JNZ27_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY8664233.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRSRQFLEKLLLQ"
     gene            complement(12712..13380)
                     /gene="tcyL"
                     /locus_tag="JNZ27_001878"
     CDS             complement(12712..13380)
                     /gene="tcyL"
                     /locus_tag="JNZ27_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY8664234.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13395..14381)
                     /gene="dcyD"
                     /locus_tag="JNZ27_001879"
     CDS             complement(13395..14381)
                     /gene="dcyD"
                     /locus_tag="JNZ27_001879"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY8664235.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14486..15286)
                     /gene="tcyJ"
                     /locus_tag="JNZ27_001880"
     CDS             complement(14486..15286)
                     /gene="tcyJ"
                     /locus_tag="JNZ27_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8664236.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15374..15925)
                     /gene="fliZ"
                     /locus_tag="JNZ27_001881"
     CDS             complement(15374..15925)
                     /gene="fliZ"
                     /locus_tag="JNZ27_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY8664237.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15971..16690)
                     /locus_tag="JNZ27_001882"
     CDS             complement(15971..16690)
                     /locus_tag="JNZ27_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY8664238.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16855..18507)
                     /locus_tag="JNZ27_001883"
     CDS             complement(16855..18507)
                     /locus_tag="JNZ27_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY8664239.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18773..20179
                     /gene="fliD"
                     /locus_tag="JNZ27_001884"
     CDS             18773..20179
                     /gene="fliD"
                     /locus_tag="JNZ27_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY8664240.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20204..20614
                     /gene="fliS"
                     /locus_tag="JNZ27_001885"
     CDS             20204..20614
                     /gene="fliS"
                     /locus_tag="JNZ27_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY8664241.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20614..20979
                     /gene="fliT"
                     /locus_tag="JNZ27_001886"
     CDS             20614..20979
                     /gene="fliT"
                     /locus_tag="JNZ27_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY8664242.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21057..22544
                     /gene="amyA"
                     /locus_tag="JNZ27_001887"
     CDS             21057..22544
                     /gene="amyA"
                     /locus_tag="JNZ27_001887"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY8664243.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22578..22991)
                     /locus_tag="JNZ27_001888"
     CDS             complement(22578..22991)
                     /locus_tag="JNZ27_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8664244.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23178..24383
                     /gene="yedE"
                     /locus_tag="JNZ27_001889"
     CDS             23178..24383
                     /gene="yedE"
                     /locus_tag="JNZ27_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY8664245.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24380..24613
                     /gene="yedF"
                     /locus_tag="JNZ27_001890"
     CDS             24380..24613
                     /gene="yedF"
                     /locus_tag="JNZ27_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY8664246.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24720..25390
                     /locus_tag="JNZ27_001891"
                     /pseudo
     CDS             24720..25390
                     /locus_tag="JNZ27_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25430..26638)
                     /gene="tnpB"
                     /locus_tag="JNZ27_001892"
                     /pseudo
     CDS             complement(25430..26638)
                     /gene="tnpB"
                     /locus_tag="JNZ27_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            26706..27321
                     /gene="tnpA"
                     /locus_tag="JNZ27_001893"
                     /pseudo
     CDS             26706..27321
                     /gene="tnpA"
                     /locus_tag="JNZ27_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27649..28731
                     /locus_tag="JNZ27_001894"
     CDS             27649..28731
                     /locus_tag="JNZ27_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY8664247.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29220..>29417)
                     /locus_tag="JNZ27_001895"
     CDS             complement(29220..>29417)
                     /locus_tag="JNZ27_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8664248.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         7416 a         7406 c         6887 g         7708 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgaaag
      301 catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac cgtagaagcc
      361 ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac caataccgat
      421 gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa gtacggtgct
      481 gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca gcattgagtt
      541 ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat aaatttgttt
      601 ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt
      661 atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc agccacgttt
      721 ctgcatctct ttatacagtt cctggccctg acgttcgcca attttagtcg ccgccatcat
      781 caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt catccactgc
      841 aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc
      901 cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa
      961 tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat ccttcccggc
     1021 tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct tcaccagaaa
     1081 accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca gaccaatggc
     1141 tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag
     1201 ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc ttttatctaa
     1261 aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat aggagagcaa
     1321 tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa gacataagga
     1381 aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata agattagcct
     1441 ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc agtcaatagg
     1501 ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca aagatgtgga
     1561 aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta acagaacacg
     1621 tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc acttcatcat
     1681 gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat taaaattcat
     1741 tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat gatttaagca
     1801 ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac
     1861 gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga
     1921 tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat
     1981 gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca
     2041 cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca
     2101 ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc
     2161 tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact
     2221 gttccaaaaa acgatggaag aatacgagca acgttcaagc acgctggcgc agttagccga
     2281 tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga
     2341 acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact
     2401 tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct
     2461 gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag aaaccgctgc
     2521 ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt aaacttgagt
     2581 tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcagcggga
     2641 tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac ttcaacgggc
     2701 gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc taccacatca
     2761 gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt
     2821 gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg
     2881 acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg
     2941 tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc
     3001 gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata atatgacaac
     3061 catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt aatgttataa
     3121 aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca agaaatagcg
     3181 cctgccaggc atcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
     3241 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
     3301 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
     3361 gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg ttatgtcagt
     3421 cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat cccctttggt
     3481 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
     3541 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
     3601 aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat tcgtattttt
     3661 aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc
     3721 cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa
     3781 aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg
     3841 cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg
     3901 aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc aatttaccgc
     3961 gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg
     4021 aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct catgctgcaa
     4081 tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct gagcagcatg
     4141 aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg
     4201 aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc
     4261 cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact ctgatcggtc
     4321 gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc taccagctct
     4381 cccgctgtca tcccttctgc cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct
     4441 ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat catcatattt
     4501 aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg
     4561 attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttat
     4621 atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa cctgcgccgc
     4681 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     4741 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     4801 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     4861 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     4921 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     4981 tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa ttgttggcct
     5041 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     5101 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     5161 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     5221 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc
     5281 cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc agctatatgg
     5341 atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc cccctggtgg
     5401 catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat
     5461 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtgccct
     5521 ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     5581 tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg
     5641 ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     5701 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     5761 cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     5821 accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     5881 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     5941 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct cttaatgcag
     6001 gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac gccccggttt
     6061 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtatgc
     6121 atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     6181 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
     6241 tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
     6301 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
     6361 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
     6421 agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca catagtcagc
     6481 cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag aactcaacac
     6541 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
     6601 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
     6661 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
     6721 tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca
     6781 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt gattttattc
     6841 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
     6901 ttttaaatcc cttgtgtcta ccgattccac catccgggct tgggaagaaa gtggaggcgc
     6961 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
     7021 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
     7081 ggagcgggaa acgagactcg aactcgagac cccgaccttg gcaaggtcat gctctaccaa
     7141 ctgagctatt cccgtattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
     7201 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
     7261 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
     7321 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt gccgcagcca
     7381 caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg
     7441 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg gccacgctac
     7501 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
     7561 tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt accagcacca
     7621 tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca aaccgggtac
     7681 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
     7741 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
     7801 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
     7861 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
     7921 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
     7981 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
     8041 acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg
     8101 ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac
     8161 atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct caaagaacag
     8221 cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt
     8281 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
     8341 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
     8401 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
     8461 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
     8521 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
     8581 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
     8641 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
     8701 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
     8761 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
     8821 gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc
     8881 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
     8941 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc
     9001 ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
     9061 acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc taatgagttg
     9121 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
     9181 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
     9241 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
     9301 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
     9361 atattcacct ttggcatgct ttgcaccacg atgcatcgcc atacgcgggt gggtatcgcc
     9421 actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg
     9481 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt
     9541 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
     9601 gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
     9661 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
     9721 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
     9781 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
     9841 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
     9901 ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
     9961 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatatcagaa
    10021 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
    10081 gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg taaagggttt
    10141 tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc
    10201 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
    10261 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata
    10321 ccctttatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
    10381 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
    10441 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
    10501 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
    10561 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
    10621 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
    10681 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
    10741 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
    10801 gccgccttaa catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag
    10861 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
    10921 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
    10981 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
    11041 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
    11101 tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    11161 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    11221 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    11281 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    11341 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    11401 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    11461 gtcctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    11521 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    11581 tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    11641 ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    11701 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    11761 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    11821 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    11881 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    11941 cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac tggcgggagc
    12001 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    12061 ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa
    12121 tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact tcgcccacca
    12181 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    12241 gtgcacgcgc aatcgcaaca cgctgctgtt gacctccaga caaacgacgt ggatagctgg
    12301 tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt
    12361 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    12421 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    12481 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    12541 ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt ttgccggaac
    12601 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    12661 gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg
    12721 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    12781 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    12841 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    12901 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    12961 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    13021 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    13081 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    13141 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    13201 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    13261 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    13321 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    13381 gagcgttccc ggagttaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    13441 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    13501 cccgccatcg cttttccggt atacacagga tcaagcagaa taccttccag acgcgccagc
    13561 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    13621 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    13681 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    13741 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    13801 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    13861 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    13921 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg ggcattggga
    13981 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg
    14041 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    14101 tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga ctggatcgcg
    14161 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    14221 ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt gatgaaaatt
    14281 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    14341 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgtgtgt
    14401 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    14461 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    14521 aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc accgctttca
    14581 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    14641 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacgatcaa
    14701 cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg tcatcatcat
    14761 aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    14821 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    14881 cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt
    14941 tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    15001 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    15061 ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    15121 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgcgc tctttgactt
    15181 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgc
    15241 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    15301 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    15361 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    15421 cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    15481 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    15541 cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga acgacatatt
    15601 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta aagccgataa
    15661 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    15721 cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa
    15781 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    15841 ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    15901 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    15961 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc tggctgtgta
    16021 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    16081 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    16141 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    16201 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca tcgtaggaga
    16261 agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata tcgatcccta
    16321 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    16381 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    16441 gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta aatgccgttc
    16501 cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc gcctgtagca
    16561 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca
    16621 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    16681 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    16741 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa
    16801 aagccgaatg taaaaaaccc cgtcggtggc ggggtttgag aggcattcag acgattaacc
    16861 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    16921 ggcctgcagg ataatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    16981 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    17041 ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga tggagctgat
    17101 ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt cagcatctgt
    17161 cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag tcactgaacc
    17221 atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt tgttatcaac
    17281 ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc tataacctgc
    17341 agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag ctgcctgatc
    17401 aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat taccggtaaa
    17461 ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt
    17521 ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc gcccagttgt
    17581 atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc tgatctgaac
    17641 atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa ctcccggggt
    17701 caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt atgacttact
    17761 tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt tattaatggt
    17821 tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt tagcagcatc
    17881 gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag ctaccaagtc
    17941 agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa acccattcag
    18001 ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc cgtcattcgc
    18061 accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt
    18121 ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct gaatggagtc
    18181 cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac ggatacgctg
    18241 taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc
    18301 gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat
    18361 cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag acagacgctc
    18421 gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga tcagcgagag
    18481 gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag tcgttgatta
    18541 cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa
    18601 acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga aatactcttt
    18661 tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt gcgcaattca
    18721 ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc gcatggcaag
    18781 tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg atagcctcac
    18841 cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt ttaccgctaa
    18901 acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg ccaatactgc
    18961 attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca ccgcgttcag
    19021 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
    19081 gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg cgatcgccac
    19141 cagcgacagc aaactcacca ttcaacaagg cggcgacaaa gatccgatta ccattgatat
    19201 cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcggg
    19261 cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag tcacatcaaa
    19321 tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg cgctacaaag
    19381 ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg tcgccgccca
    19441 gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca ctatcagcga
    19501 cgcgctggaa aacatcaccc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
    19561 catcactcag gacacctcca aagcgcaaac ggcgattaaa gactgggtga atgcctataa
    19621 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
    19681 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
    19741 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgctggcgca
    19801 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
    19861 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
    19921 aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
    19981 tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta aagactacaa
    20041 cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat ttacccaact
    20101 ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc agttcgaaaa
    20161 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
    20221 cacccaggcc tatgcgcaaa ttggcgtcga aagcaccgta atgagcgcca gccaacagca
    20281 gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gactgtttat
    20341 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
    20401 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc aaaacttaat
    20461 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
    20521 cgcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga aagagtcttt
    20581 actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca tttgtatttc
    20641 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
    20701 tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac
    20761 ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc gatgctgcac
    20821 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
    20881 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta tggcgatcag
    20941 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag ccgatggctc
    21001 gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt cgaagaatgc
    21061 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
    21121 ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg gtctggttgc
    21181 cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc tatgatttat
    21241 ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt gataaagcac
    21301 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg
    21361 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg
    21421 ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca
    21481 ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa tgttttagcg
    21541 gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt aacgactata
    21601 ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    21661 gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    21721 gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa catattccgg
    21781 catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    21841 ttgtggctga atactggtcg catgaagtcg ataaactgca aacgtatatt gatcaggtgg
    21901 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    21961 tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa gccgatcctt
    22021 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    22081 cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg
    22141 ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc ggtggtgacg
    22201 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    22261 gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac tgcattgcct
    22321 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    22381 atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg cgtgattttc
    22441 tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca
    22501 acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat ccccggcggc
    22561 aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg
    22621 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    22681 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    22741 gacatcaatg tcaacttacc cggcacggca atccctcgct gccactgacg gcaatccaag
    22801 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac cagcgcacgt
    22861 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    22921 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    22981 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagctctga
    23041 tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt ttgtgaatta
    23101 ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca aaccttctga
    23161 gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg attaaattct
    23221 gggctcccat ccctgcggtc atcgcggcgg gtattctctc cacttactat tttggcatta
    23281 ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag ctcctgcagc
    23341 tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg gaaggatcgc
    23401 cattaacccg catcgacggc atgatgatcc tcggtatgtt tggcggctgc tttgctgctg
    23461 cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc attatgcagg
    23521 ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc tgtaacctgg
    23581 cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt gccatcgcca
    23641 ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc cgtattcccg
    23701 tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat caggcgcggc
    23761 gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg ctgctcacgg
    23821 cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt ggtttactga
    23881 ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc accggacgta
    23941 cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg atcttcagtt
    24001 acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg gtaattggtg
    24061 gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc tggatgtacc
    24121 gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg atcggctcaa
    24181 cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac tgggacaaaa
    24241 tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat ttgctgctgt
    24301 ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt gcggcaccgc
    24361 agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg atatggtggg
    24421 tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat taaaaaaagg
    24481 tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc cactggatgc
    24541 gcgtaatcac ggctacactg tgctggatat tcagcaagac gggccgacca ttcgttattt
    24601 aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg ttacctctga
    24661 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggttgcta
    24721 tgtgtggacg ctgtgcccaa tcccaaacgc gtgaggatta ccttgcgctt ctcgcggaag
    24781 atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat gtcgcgccgg
    24841 gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    24901 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    24961 cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    25021 atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta tctatcgcgc
    25081 tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc gtggtgacga
    25141 agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag atattcatga
    25201 ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc aagacattgg
    25261 tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc agttcacctg
    25321 gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt taattcaacc
    25381 tgtttgttga catcctccac gccctgaatg acgaggcccc ctgctacgtt caggctgtcg
    25441 cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc atctctccac
    25501 aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc gccatttaca tcggcgttcg
    25561 ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt tctttcgctg
    25621 tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca agcacctgac
    25681 cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag ccctgatcca
    25741 gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg ctgaccgtac
    25801 ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc attacgtggt
    25861 ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt gcgatacagg
    25921 aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat ttgaccttgc
    25981 ggcttaactg tcgctgaagt ctcgccagct tcttctggtt tttctgaaaa ctgtttacag
    26041 gctcaaagac tgtgccatct gacagcgagg caagtttagc cacgccagca tccagcccga
    26101 ccattgatgc tgaagggtga accggagttg atacttcact ttctgcctga atactgatgt
    26161 accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg acctgacggc
    26221 tgttccggta gcgcatccag ccaagttttg gcagaaaaat acggctgttt tcctgatcga
    26281 gcttaacgcc ctgcggatag cggaatgcat cattctgtca ccgctttttg aatcagggga
    26341 acgccgaccg cttctggaaa aagtttttgc aggcctgctc agggtctttc agtgactgtt
    26401 gcaatggctg tgacggagca tctttaatcc attgcgtttc agtggcattt ttccactcaa
    26461 ccaatcagga agccattttc gcgtaaggga tgtctttgtt accggcctta tgattctcat
    26521 tctgacgtgc cagagtacga ctgaaaacga aacgacatgc gcctgcgaag cgcctcattt
    26581 cacgcgcctg ttgaccaccg ggtcttaacc ggaatttgaa tacctgtagt cgtttcacag
    26641 ggcaaattat actttggtct gtgaaaaaaa ctgatatccg acgcggaagg cactgtgttt
    26701 tcctgatgca tgctcaccgg gtctttgtta ccaagtaccg acgccagatt tttgaccatg
    26761 acgcgacaga aaaactacgt acttactttt caaatgtatg tgctgatttt gaagctgaac
    26821 tggttgaaat ggatggcgaa ccagagcacg tccatctgtt aattaactat ccccccaaac
    26881 tggcgatatc cagtctggta aacaaacagc ctcaaaggcg tatcgggtag gttactgcga
    26941 cgggatcggc cagatattgc tgccaggtat taccgcaaag atgttttgtg gcgtcccggc
    27001 tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa
    27061 cagcaaacac ctggtcaggt gaaaaaacga gccttatatc cccgccctga aggacgggct
    27121 tttacggtgc actggataag cctggagatt atctacacct gcatggacat cattgtacta
    27181 tgccccgtac atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt
    27241 atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc
    27301 gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa ataaatatca
    27361 tgcaaataat ttccatggtt atttttaggc tatttaaatt aaaaaaataa attatgagtc
    27421 aatcataaat aacaaccttt aatataattt gactaccaaa tttatattcg ttcacaaaat
    27481 agtcatgcat tgtgtgcaat agaaacagtt cagataaaaa tagggatcta cttcacaatt
    27541 taaatgagaa actaaaactt acatcttgaa ataatctcat tgattggatg aatatttgta
    27601 gtgcagtgac atcattttta aataatagtt caaataaaag ggctcacgat gaaaaaatta
    27661 acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc tcaggcagca
    27721 gagatttaca ataaagacag taacaagctg gatctgtacg gaaaagttaa tgctaagcac
    27781 tacttctctt ctaacgatgc agatgatggc gacacaactt atgttcgtct gggcttcaaa
    27841 ggcgaaactc aaatcaacga tcaactgact ggtttcggtc agtgggaata tgaattcaaa
    27901 ggcaaccgtg ctgaatctca aggttcttcc aaagataaaa cccgtcttgc cttcgctggc
    27961 ctgaaattcg gtgactacgg ctccatcgat tatggccgta actacggtgt agcatacgat
    28021 atcggtgcat ggaccgacgt tctgccagaa ttcggtggcg atacctggac ccaaacagat
    28081 gtgttcatga ctggtcgcac cacaggtgtt gcaacctatc gtaacaacga cttctttggt
    28141 ctggttgatg gtctgaactt tgctgctcag tatcagggta aaaatgaccg cactgacgta
    28201 actgaagcta acggtgatgg tttcggtttc tccactactt atgagtatga aggattcggt
    28261 gtaggtgcaa cctatgctaa atctgaccgc actaatgatc aggttatcta cggtaacaac
    28321 agcctgaatg catctggtca aaatgctgaa gtatgggcag ctggtctgaa atatgatgcg
    28381 aacaacatct atctggctac cacctattct gaaacccaga acatgactgt ttttggtaat
    28441 aaccatattg ccaacaaagc acaaaacttc gaagtagttg cacaatatca gttcgacttc
    28501 ggtctgcgtc cgtccgttgc ttacctgcaa tctaaaggaa aagacttggg tgcgtggggt
    28561 gatcaggacc tgattgaata tattgatgta ggtgcaacct attacttcaa caaaaatatg
    28621 tccacttttg ttgattacaa aatcaacctg attgataaga gcgatttcac gaaagcatct
    28681 ggcgttgcta ccgatgatat cgttgctgta ggattggttt accagttcta atctgattac
    28741 tcaaagatat gttgtgggag gctttgcctc cccaacatat aagtggcgcc ctcaagccac
    28801 tttccttaga agcactatct tgcttctgac tatataaatt accctttatt ttctgggacg
    28861 tttcaacgcc ccatttttaa taatactcag tgaacaatta acgtatcaat tagatttaat
    28921 aacaacgata tccgtaccta acctgatatc taatgtcata agcatccctt taattatccc
    28981 ctcaacctct tgtaaatttc ccactcttga gctccacata gtccaaacgc cggatgcaaa
    29041 aaaaatcaca cgtgcatctc tgaaattgtg atggaggaag tgagtatcaa ttatatgtgc
    29101 ttataaacgt tcccgattgg cacagaagtc accggattgt tcaggctcca ataggttgag
    29161 tatgtcgtat ttattttcca aaataaaaat cgatgctgtt gctccttaat aaaatacttt
    29221 tactctctgg acgcttttct tagctctctt ggtgttatct taaaacgatt atggaacctt
    29281 tcagtaaaac gagaaggaca cttgtaacca ttttccctgg caatctcgct gatgggtttt
    29341 actgtcgttt gtatagcaga caacgcatta tttaacctca cgtcgtccaa tatactttga
    29401 aaacttattc cctcgct
//
LOCUS       DADMFL010000043        29348 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.043, whole genome shotgun sequence.
ACCESSION   DADMFL010000043 DADMFL010000000
VERSION     DADMFL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.043"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..105
                     /locus_tag="JNZ27_001896"
     CDS             <1..105
                     /locus_tag="JNZ27_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'') family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HAY8664249.1"
                     /translation="QAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            181..678
                     /locus_tag="JNZ27_001897"
     CDS             181..678
                     /locus_tag="JNZ27_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664250.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            739..1110
                     /locus_tag="JNZ27_001898"
     CDS             739..1110
                     /locus_tag="JNZ27_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664251.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            1520..1897
                     /locus_tag="JNZ27_001899"
     CDS             1520..1897
                     /locus_tag="JNZ27_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664252.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            1920..2072
                     /locus_tag="JNZ27_001900"
                     /pseudo
     CDS             1920..2072
                     /locus_tag="JNZ27_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2128..3744)
                     /locus_tag="JNZ27_001901"
     CDS             complement(2128..3744)
                     /locus_tag="JNZ27_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY8664253.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3745..5271)
                     /locus_tag="JNZ27_001902"
     CDS             complement(3745..5271)
                     /locus_tag="JNZ27_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY8664254.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(5274..6941)
                     /locus_tag="JNZ27_001903"
     CDS             complement(5274..6941)
                     /locus_tag="JNZ27_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8664255.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(6938..9046)
                     /locus_tag="JNZ27_001904"
     CDS             complement(6938..9046)
                     /locus_tag="JNZ27_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY8664256.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(9033..9854)
                     /locus_tag="JNZ27_001905"
     CDS             complement(9033..9854)
                     /locus_tag="JNZ27_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HAY8664257.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(10014..11843)
                     /gene="glmS"
                     /locus_tag="JNZ27_001906"
     CDS             complement(10014..11843)
                     /gene="glmS"
                     /locus_tag="JNZ27_001906"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY8664258.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(12005..13375)
                     /gene="glmU"
                     /locus_tag="JNZ27_001907"
     CDS             complement(12005..13375)
                     /gene="glmU"
                     /locus_tag="JNZ27_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY8664259.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPLGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(13727..14146)
                     /gene="atpC"
                     /locus_tag="JNZ27_001908"
     CDS             complement(13727..14146)
                     /gene="atpC"
                     /locus_tag="JNZ27_001908"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY8664260.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(14167..15549)
                     /gene="atpD"
                     /locus_tag="JNZ27_001909"
     CDS             complement(14167..15549)
                     /gene="atpD"
                     /locus_tag="JNZ27_001909"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY8664261.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(15576..16439)
                     /gene="atpG"
                     /locus_tag="JNZ27_001910"
     CDS             complement(15576..16439)
                     /gene="atpG"
                     /locus_tag="JNZ27_001910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY8664262.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(16490..18031)
                     /gene="atpA"
                     /locus_tag="JNZ27_001911"
     CDS             complement(16490..18031)
                     /gene="atpA"
                     /locus_tag="JNZ27_001911"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY8664263.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(18044..18577)
                     /gene="atpH"
                     /locus_tag="JNZ27_001912"
     CDS             complement(18044..18577)
                     /gene="atpH"
                     /locus_tag="JNZ27_001912"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY8664264.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(18592..19062)
                     /gene="atpF"
                     /locus_tag="JNZ27_001913"
     CDS             complement(18592..19062)
                     /gene="atpF"
                     /locus_tag="JNZ27_001913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY8664265.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(19124..19363)
                     /gene="atpE"
                     /locus_tag="JNZ27_001914"
     CDS             complement(19124..19363)
                     /gene="atpE"
                     /locus_tag="JNZ27_001914"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY8664266.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(19410..20225)
                     /gene="atpB"
                     /locus_tag="JNZ27_001915"
     CDS             complement(19410..20225)
                     /gene="atpB"
                     /locus_tag="JNZ27_001915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY8664267.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(20234..20614)
                     /gene="atpI"
                     /locus_tag="JNZ27_001916"
     CDS             complement(20234..20614)
                     /gene="atpI"
                     /locus_tag="JNZ27_001916"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY8664268.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(21231..21854)
                     /gene="rsmG"
                     /locus_tag="JNZ27_001917"
     CDS             complement(21231..21854)
                     /gene="rsmG"
                     /locus_tag="JNZ27_001917"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY8664269.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(21918..23807)
                     /gene="mnmG"
                     /locus_tag="JNZ27_001918"
     CDS             complement(21918..23807)
                     /gene="mnmG"
                     /locus_tag="JNZ27_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY8664270.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(24186..24629)
                     /gene="mioC"
                     /locus_tag="JNZ27_001919"
     CDS             complement(24186..24629)
                     /gene="mioC"
                     /locus_tag="JNZ27_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY8664271.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(24719..25177)
                     /gene="asnC"
                     /locus_tag="JNZ27_001920"
     CDS             complement(24719..25177)
                     /gene="asnC"
                     /locus_tag="JNZ27_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY8664272.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            25329..26321
                     /gene="asnA"
                     /locus_tag="JNZ27_001921"
     CDS             25329..26321
                     /gene="asnA"
                     /locus_tag="JNZ27_001921"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY8664273.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(26326..27777)
                     /gene="viaA"
                     /locus_tag="JNZ27_001922"
                     /pseudo
     CDS             complement(26326..27777)
                     /gene="viaA"
                     /locus_tag="JNZ27_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(27771..29255)
                     /gene="ravA"
                     /locus_tag="JNZ27_001923"
     CDS             complement(27771..29255)
                     /gene="ravA"
                     /locus_tag="JNZ27_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY8664274.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWRLLQASAFFSGRSAVAPVDLILL
                     KDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTL
                     IRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGE
                     IRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELE
                     SDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         7368 a         7556 c         6856 g         7568 t
ORIGIN      
        1 caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
       61 gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct aacaattcgt
      121 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagagagct ggggaagact
      181 atgcgcgatc tgttgaaggt ggttctaagc ctcgtacttg cgatggcatc ggggcaggca
      241 cttgctgacc tgccaattgt tttagtggat gaagctcgtc ttccctatga ctactcccca
      301 tccaactacg acatttctcc aagcaactac gacaactcca taagcaatta cgacaatagt
      361 ccatcaaatt acgacaactc tgagagcaac tacgataata gttcatccaa ttacgacaat
      421 agtcgcaacg gaaatcgtag gcttatatat agcgcaaatg ggtctcgcac tttcgccggc
      481 tactacgtca ttgccaacaa tgggacaacg aacttctttt ccacatctgg caaaaggatg
      541 ttctacaccc caaaaggggg gcgcggcgtc tatggcggca aagatgggag cttctgcggg
      601 gcattggtcg tcataaatgg ccaattttcg cttgccctga cagataacgg cctgaagatc
      661 atgtatctaa gcaactagcc tgctctctaa taaaatgtta ggcctcaaca tctagtcgca
      721 agctgagggg aaccactagt gtcatacgaa cctccaagag acggttacac aaacgggtac
      781 attgttgatg tcatgtatga caatcgccca agtaagtatc cagctgtgtt cagaacgtac
      841 gtccgaattc cagactatgt cgaactagat aacgcggagg agctggccga cgcaatcatt
      901 cgagaggttg agttaaagga ggcatggctc acaagggtct tggggaactc agccgatcct
      961 gacgcttctg tgggcaagtg ggaccggcgc ctagcgcagt gcggtggtat tgatgagatg
     1021 tcaagtgggg tgcaggcaac atacttcagt ccaaacggtc agctggtcgg cttagtggca
     1081 tacattgatg ccgaatggat ggatgaatag ctctgacgtt acgattcagg tgagacaacc
     1141 agatgggcat tgcgtacaag ctcagttgcc accgatgtgg ctacgagtct gatcttcttt
     1201 acctaggcca aggtatggcg atgcttcctg agcaaatagt cggaacgtgc acatgctgcg
     1261 acaatctaac gacgatttct ataaatgaaa gctacggagt atgctcactt tgtggggcta
     1321 aggatcgtgt gatatttaca agctatcagc atgaggcaag gccaagaaat ccctgctatc
     1381 tagagagaag tttcaaatat caatgcccaa gatgccatgc cttcagtatg gagcctccgg
     1441 atctaccgga gatgctttgg gactaatgct ccgctttgcc tcgggtatcc gttcgaccta
     1501 tgtttaagag gaaacgcacg tgtctgccgc caaagctctt gcctatgcac tttgtgacaa
     1561 gttggaaagc aaaaaagttg atgaagtcgt acgaaatctt gtttctagcg gctggaatat
     1621 tagggaagtg gcctgggaag cttcgtcctt tgatcatgaa atgccctact ttgacacggg
     1681 gcgggacttc gatattggat ttcctgacac gctgtcatcc aagattgagt ttttgaccgc
     1741 gaatggatca atcgtccaag cacgtgctcg cctgcacttc aagcaggctc taatttttgc
     1801 caaggcgtcc aaacatgtcg ccgcgctcaa gaactcattg gattactact atggatcgtc
     1861 tgtaatcccc cctcaaaacc acggaggcta tttgtagaaa attccattaa tatgaactca
     1921 aggtgttccc tacggacgaa tcagcgatga aggtggtgta cctggctatc caggcggcga
     1981 ccaagaaatg gacgatgccg atccgcaact ggaagccggc catgaaccgt tttatgatcg
     2041 aatttggtga ccgtttaaat ggccaccttt aactgaccgg caaaagcact tacacaaaag
     2101 ggtttacagg ctctttcagt cattccttta atgcgtaaat tgctctctaa ccactctctc
     2161 agcccaccct tccatatcct ctctcgatac ttgcagctcc cccaacccct ttggatgatt
     2221 cacccctcta tgcccgtctt gtccatataa ttgatcaaac aaactatttg gccaattcaa
     2281 tgagctcttc accactccac gccgtatctt ttcaaaatca ttccgccatg tttcgctatc
     2341 aacgccactc aacactttgg tagaaagcat tttcacccca tccgatgcat ccacctctag
     2401 caatacaaat tcactgccat tacgttgcac cccaacggct ttaatcactc ttggtgagcc
     2461 atctttacac agatgtaatc ggctacgccc aacttttggc aaaaccaagg tttcttcaaa
     2521 aagcacccga catgcaaatt tagtttttag aattgaaagt agctcatcaa aagcagcaaa
     2581 tcgattagca aaaatagagt tgtaattggt tgcatcctgc ttcccaccca catctgccgc
     2641 tgctaggaca ccacctaagt gtggctcatc agtactaacc aaattactat tttcacttgt
     2701 tgttgcttct tctttttcta aaatagtttg gctacttttt cttgccctgc gagatttttc
     2761 aattcgacta ggtctactaa aacttatcca tgtcggctca gcctctatga ctcgtgtgtc
     2821 tgtgtctata ttggctgttt cctcatcgtc caactgtaga tgctcatcat caacaactga
     2881 ctctgttgaa accgctatgt gctgcgtact accatcacct gcctcctttt cctgaaaaga
     2941 ggcatggcta atagctgttg tgctaggaag catagcgttg atttctaaac ctactatttc
     3001 ctcaactaaa taatccttat cctcgttaga agaacgtcct tttacatgta atttccaacc
     3061 ctgcatgggc ggagggtcaa agctaaagca ccagctttca acgccgttct taatttctct
     3121 attttgttga tagtgcctaa aaatactttc atacgaatcc ataacatctt gatccgaaaa
     3181 caaccaaacc aaaagctgca ctacggccga ctgctctaac gcccctttag gaaacgatga
     3241 actgggtaag atccttatct ctaaatgatc tcgctcaacc tcatactgaa cgtcgaactc
     3301 ttgctgtaaa gcggtactgc tcaaacagct tcgacagaaa taggagttaa ttaaaaataa
     3361 tgaccgagct agctctaact gtgggatgtg ataacgagtc ttgctatatg gtgtttcagc
     3421 ttcgtaaata aatgaactct gcgctccatc cttgttgcgt acggaagcaa aggacgagag
     3481 attaggaaaa tcgattattt tggctcgttg aagatccgat gcttcaaact caatcacacg
     3541 atccgcctta tttatttgct ttgttgtact gttaaccacc ttagctcgac ttaataaagg
     3601 caaatgagta aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa caaaaatacg
     3661 ccactcctta tgacccgagt tacggaataa atgaccaata tgtacaacct gcacattgtc
     3721 attaaatgta gctagcctaa ccactcactc ttccccataa accatttcca agaatctttg
     3781 tgcttcctca gttatccgct ctttagataa cgttgcactt cttaataatc gccagcgcct
     3841 aagctcaaca tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc taatttgata
     3901 atcttccaca ctctctgagt aacgctttaa gcaaagcgct accaaaggca gtttctgtag
     3961 attttttgca agagaggttc cgttaggagt ttgctttaac agccagctcg atgtcgctct
     4021 tgggtgatca aggctactat ctagacgctt tatgattctt aatagttgtc gtacagcaat
     4081 tcgatctctt tggttccaat caactctagg ggcgggtgcc agacgctctt gttgatgctg
     4141 ttgattccag tgaactagcc aatccctgtc atgtcggtaa agccaagcgt atagcacccc
     4201 accctctaaa gactgtcttg ccgccttaat tccttggtat ttatgcacta gttgctgcca
     4261 gtctttatgc ttaacagata aatcttcaga gttaggttgc acagactggc taacaggtct
     4321 tgtcgttata gagtgctcag taagagcact tgcctgctgt agcgcttcta taaccgttag
     4381 ttttggcaat aaggcttgcc acacaatact atgctgtaaa taactaaagg cttttctatg
     4441 cttacggaat atacttttca gccaacacgt gtccttgttc tctgccaact ttaaatccag
     4501 tttctctagt gcctcatcac taaaagtctg cctcactctc tccgccacca agtcatgacg
     4561 aatgtgcttg cttttggtta gccctagatc ctgcgctaag cgctgataaa acagcgtcca
     4621 ctgctcaagg cttggggaaa gctcttgcgc tcgtggagca tctaacagag gggctatata
     4681 agcagctagt gctgttaatt gagataggga gtctttgggg tagtctgaaa gcagctcagt
     4741 atgacccaaa gcccaaaatt gatgtcggtg atcatctaca gctctatcaa agaagactaa
     4801 agcaccgtgt tttggacaat atggcaaagc gggcaaatac caatctcgtt gccaaaaggc
     4861 ttccccatac ctatttagct gaagagcaac gcaatcaggg cagtatctaa agcggttatc
     4921 gctcttaact ctagaagcag cgactcctag cattaaatgc accgcacctt gcgcttggta
     4981 ctccattaac cgaatagctt cgtctcggcg ctccttgcct acaaacggag catataaagg
     5041 gaataaggta tgctcataaa taagctgctg aacagcgtaa cgtcctgttt gatgtagatg
     5101 tcttgctatc acacctaaat gcgagggcag acctaaggta gcgaccacct tgcggttgcc
     5161 atacacctca tccaacagct gcttaggact aacaatccct tgataaacgc ctgcccgtgc
     5221 aatagtgcta taaatcagct cattcgagta cggaacagga aagtttctca tggctaacct
     5281 gcttgtttaa ataagctcgc catatccact attacccctt tcccttttaa ccgttcatgc
     5341 atggtttttt caggttggcg ttgtgaatag atataacgta aatccgtatc aggcaagcta
     5401 tcccagtcgc tgggcttgac tacctttata gccgaaacct ttttactctt ggagggcttt
     5461 tctagttctg acactaccgt ttcgccttcc atcaaccact gcaaaacaag aggcagtaac
     5521 ttttgtcttg tcatcgttgg attctggcta aacgcttttt taatagtggg aattaacagg
     5581 cttgaatcgt aatcctcttt cagcatcaga tataaatgac gctgatcttc ggtatctaac
     5641 tcttgaaggg ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc tagctgaagt
     5701 tggattaacc gtttatcaat ctcgggaacg actagatcag aataacgagc aatgcgttct
     5761 gggatacccg agcgtaatgc ctctagcatg gggtgcacag gctttaactc atcttgatac
     5821 acttgccgca ataaaccagc ggtaatacgc tcattgccta aagctagcgc acggagctga
     5881 gcgagtacaa aaagttttac tacaatgtcc atcactcctt ggcttagctc ataccacaca
     5941 tcacggacct catccgataa cagcgcatct ttgcgttgta aaagctgtaa ttgccaaaga
     6001 ttatccgtaa aagcgatcca ctcttgattg ggctttccac gttgcgtttg ttgtatagga
     6061 tcccagaata tagctccaaa ccctgcccct ctacgtgcag accgcaaatc agcctcaaaa
     6121 atctctcgtg ctttaggggt accaatcaac atcactggta cgccaataat attcaccatc
     6181 gtcacaaaaa agttcagcat ctcttgagat ccacccgaac gagagcggct taaatgctga
     6241 atttcatcaa taaccaacaa ccctaaagca tgtgcattgg ctatttgcga catcaaagcc
     6301 aacatggttt ctataccatg acgttttaag ccataacgac gctcatagtt cgagcccaag
     6361 gctcgatcca acgctctgaa aaaattcaag cagatttctt ttagcgaacc attatgcgag
     6421 cagtctattt tcaaatacac cacctgctct acattgagtt cacgatggta aatcacctga
     6481 ggatacgtgg ctagaatacg atgaagagag gtcgtcttcc cactaccaga acaaccaatt
     6541 aacaataagc tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc caactctccc
     6601 gtttgaacac gctcataacc attttgtaaa tgcttttgta aatctcctgt tttaggattt
     6661 ctgcctacgt agccacctcg aatcatgacc gaaatacgct cacttagtag caaatgcgta
     6721 cctaatggct gaaaatagtc atctggaata cgacaaatgg tatgagctct gataacacgg
     6781 gacttttgca agtcagagga agtaagctgt aaagaggatt tcagtgatgc agcactattc
     6841 actgactctt gtaatggtgg taaggcctcg ataaaaggat tatcacgata agcctctacc
     6901 cccgtatcac gataaactgc ttgaatccgg gtagcactca tgactcatcc ttttctggtg
     6961 gatcctgaaa taattcaggc acgtatgtgg gtaggctgta atcttcttga tcatccgctt
     7021 ccactgcgtt gaaaggaata actttagcct catcacctga agagcttggc ttcaaatgct
     7081 ccgcacgttt tttacgctcc gaggtcacgg cttctttttt attagtttta atctgcttaa
     7141 tgcgtgttga tttgggctca gtagtactgg gcgttaactt attcgctttc tgaatggttt
     7201 gctgaataaa cgcctcaagc tccctgcgtt tagttaactc atcctgcttc gcattggctt
     7261 tattgtgttt ttcttgtgct tgtatatccc aaacctccca aaatgagaga cctttaaact
     7321 gccgactacg ttccgtcaga ttacagcgcc aaaatacacg gctgccaact tgcggaaaca
     7381 aataaatcgt atcaaccagc actgggtcat aagccgcttc taaatgttga ggtctagcta
     7441 tatcagtgct ccgctgcaac caaccctcac gcagaatctc tgaccccgag taatacaaac
     7501 cccacaaatt aacgccaaat gaagaaatag agacctttcg gcgaggcagt aacgctactc
     7561 gcaactgctc ttgctccaca gcccttaaac tacctgtacg atgctgcata ccccattgcc
     7621 atagctggac aggaatagac ggtaaatctg taggaaaatc agcatctcga tcgtatttat
     7681 ccatcaccag atggttattt ctgaataaga tcgtacgcaa aataatttgt gtgaactcaa
     7741 ataccgacag agatgcatct aacctatagt ctgtttcacc atggcttttg atccgactgc
     7801 cctctacaat gccaggtgca aaggacttaa actcggcttg tagtgttcta aaagtgcttt
     7861 ccactatgcc tttagcatcg ccacgtctag gtggagcact ttccactcgc acattaaaac
     7921 tagaaactaa ggcttcgacc tgatgactca ttaattcgcc acggtccgct agcaacacat
     7981 ctggcaaacc tacacacggc cagtctgagc tactaatctc aatatcatgc tgggcacaaa
     8041 tggccgtttt gtcagagcaa gcatttacaa aagcctgcat cgccaccaca taagacggat
     8101 tttcaaagcc gatataaaag cccgtgatca tccgactaaa cacatcaatc acaatgtaaa
     8161 gcgttggtct tcctatgatt ttttggcgat catgatgatc cactaaataa atatcagcaa
     8221 tcgtggcatc aatctcataa cgactcccag ggcctaacgc ctgagaagtg gctgtactac
     8281 ttaagggtcg tacgtctttt ttatataccc ctgctttaac tctagacttt aagcgctgag
     8341 ctttagggta ttctcgatca taaaaataac gaaactgacg tagtgttggg taatcctctt
     8401 ggggaatgcg aggaaaatac tgagcaaaca agtccacaaa tcgtctatag gcaacggtgg
     8461 tctttgtacc tttttgattt aacaggtgct tttctatggt caacctaaaa aggcgttcaa
     8521 tctcgggcgt taccttggtt ccttcaccct taccatattc tctggctcgg ccaatctttg
     8581 ctgttcctgt cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag tcaggaatta
     8641 atgcattagg cgtttgacca cgctgccagt aacggcgtaa caacttataa actgtagcct
     8701 tagtaacctt atgttcttgg accacatgct caacgagttc gcttctgact ttagggtcga
     8761 aacgatcctt actattaata ataggaagaa tttttcgata atcctcctcg cgcttctgga
     8821 agctgacaga atctacagac ggctcttcta aatcaagatg tacataaggg tcatcagttc
     8881 taactaagcg gccttcatct aaatactgca ttagcaacaa ctcagcccta gcttgtggaa
     8941 ctcctttatc agcgcttatt tgcatccaga caacttggcc atcctctata gccaaaatgc
     9001 gatacggatc attatcaaat agcacaacct cattaatttg ccacatagcg caactcctcc
     9061 atattcacta cttggctaat acagagatct gcacacttat ttgccctgaa agacttataa
     9121 atattgaact taataaaacc atttgcggtt aaggctcgta tctcactcaa tgttttgcct
     9181 aactccaaat cataagcaat atcaacctgc ttacagacat tgataatgtt ttcatctcct
     9241 ttttcttgca ggatatgggc caatggggat agttgtgcta aaagctccgc agaaacttct
     9301 tctgttttca ctgaataaag ccattcaata ttttctttta ctacgggatt tatttcttta
     9361 tcagtaaaaa tgaaccaagg aatttgcttt tgctgccaat agcgacgctc tagttctagt
     9421 ttttctaagg tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat agcaaactgc
     9481 tcaaaaggac catctttgca gtccactaaa aaatcagtag acataacctg atctacacca
     9541 cgaataacag gatgcttaat accactatct attgcaatct gcctggtatc actaggtaat
     9601 aaggggaact gctcgcgtat atctagcacg ctgctctccc actcaagact gagaaaaaca
     9661 gcaagctcta agtcagatag caaatgatgg actcgtcccg tcttatgaga ataaatacgg
     9721 tgggaacgac ctgaagaagg aacttcttgt actgttagcc atggaatata gtctttacca
     9781 tgcccttggc cacgcccctc tttaatacga cgggcaattt gcacttcaga aaatgaagag
     9841 tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg gactgatttc
     9901 agtttacaac tattctgtct agtttaagac tttattgtca tagtttagat ctattttgtt
     9961 cagtttaaga ctttattgtc cgcccacaag ccctttttta tctatgatag tgattactca
    10021 accgtaaccg attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg
    10081 acatggtaag ccagcagctg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc
    10141 acatgcggca tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg
    10201 gcgaagacat acaattgacc gccacgcgcg cgaacttctt caatgttgga tttcagtttt
    10261 tccaacagtt cgttgttcgg tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc
    10321 agcgggccgt gtttcagctc gcctgcagcg taggcttcag cgtgaatgta agagatctct
    10381 ttcagcttca atgcgccttc cagcgcgatt gggtactgat cgccacggcc caggaacagc
    10441 gcgtgatgtt tgtcagagaa atcttctgcc agagcttcaa tgcgtttatc ctgagacagc
    10501 atctgctcaa tacggctcgg cagcgcctgc agaccatgca caatgtcatg ttcaatggag
    10561 gcatccagac ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactga
    10621 gtggtgaatg ctttagtgga cgccacgccg atttccgtac ccgcgttggt catcagcgcc
    10681 agatcggatt cgcgcaccag agaagaaccc ggaacgttac agattgccag cgaaccaagg
    10741 taacccagct ctttcgacag acgcaggccc gccagggtat ccgcggtttc gccagactgt
    10801 gacaaggtga tcatcaggct gttacgacgc acggcagatt tacgatagcg gaattcagag
    10861 gcgatttcga cgtcgcacgg aatacctgcc agcgattcaa accagtagcg ggaaaccata
    10921 ccggagttat aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt
    10981 tcgtcggcgt tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta
    11041 agggtgtttt ttatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa
    11101 atacctttat cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc
    11161 gcgccagttt tatcgaagat gtttaccgaa cggcgagtga tttccgcaat atcgccctct
    11221 tcaaggaaga taaagcgacg ggtcaccggc aacagcgcca gctggtcaga agcgataaag
    11281 ttttcgccca tacccaggcc aatcaccagc ggactaccag aacgtgccgc cagcagggta
    11341 tccgggtgac gggagtccat gatcactgta ccgtacgcac cgcgcagctg cgggatagca
    11401 cgcagaacag cctcacgcag agtcccgcct tgtttcagct cccagttcac cagatgggca
    11461 atcacttcgg tgtcggtttc agaaacgaag gtatagccac gcgcttttaa ctcttcacgc
    11521 agcggttcat ggttttcgat gatgccatta tgcaccacca caatgtgttc agaaacatgc
    11581 ggatgcgcgt tagcttctga aggttcaccg tgggtcgccc agcgagtgtg agcaataccg
    11641 gtgccgccat gcagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg
    11701 cgacgcaggc gagtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat
    11761 ccgcggtatt ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg
    11821 atcgcgccaa caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc
    11881 tgtatgcccg ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt
    11941 actgcttgtg ggagggagat tatgttatct cctcatccca gatgaccgga ttacccggcc
    12001 agaattactt tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc
    12061 tgatagctaa tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg
    12121 cgcctttgcc tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt
    12181 cgccgataat ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc
    12241 cgatgttaac gttatcgcca atttccgcat cgcctaggta agtcagatgc ccggctttcg
    12301 tgcctttacc cagacgcgct tttttcatct caacgaagtt accaacgtgt gcaccttcca
    12361 gcaactcagc accaggacgc agacgggcaa acgggccaat agtacaggcc gctgccagat
    12421 tcgcgtcttc cacaacggta tacggactga tttcgcaatc atcgccaatc acgctgtttt
    12481 taatcacgca accggtgccg attttcacgc gatgaccgag agtcacgttg ccctcgatga
    12541 taacgttagt atcaatttca acatcgcgcc cgtgagtaag cgtaccgcgc agatcaaaac
    12601 gcgccggatc gcgcagcata acgcctgcta acagcagttt ttcagcctgt tcggactggt
    12661 aaacgcgctc cagacgggag agttgcaggc ggttattcac accttctact tcgcttaaac
    12721 gttgcggatg aacggcgacg atttcacgcc cttcctgata cgccagcgca ataatgtcgg
    12781 tgatgtagta ttcgccctga gcattattgt tggtcagctt cgccagccag cgtttcatat
    12841 ctgcgccgtt ggcaatcaga atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg
    12901 tggcatcttt gtgctcaaca atgccggtaa ctttgccatt ttcacgggtg atacgtccat
    12961 aaccggtcgg atcatccagt ttcaccgtca gcagaccaat gccacccaga ggtttagcat
    13021 cacgcaggcg ctggagtgtt tcgacagaga tcagcggcac gtcgccgtag agcattaaaa
    13081 tgtcttcatc atcggcaaag aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct
    13141 gctctgcctg aagcacccag ttaaggttgt cgtctttcag cgcctgttta agcagatcgc
    13201 cgccgtgacc gtacaccagg tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga
    13261 catgctgaac catcgctttc ccggcaaggg tatgcagcac tttcggaaga tcggaataca
    13321 tgcgcgtgcc tttgcctgcg gcaaggatca ctacgctcat agcattattc aacatacgcg
    13381 tcctgactgt aatttgagaa cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt
    13441 tcatcgtgaa atggacagag gataaattgt tcaataacgg attatccccg ctgacgaaag
    13501 tgccattttc gaccatcgtc acgccttttg tctgcagaaa attaaggcag taccttcatt
    13561 taagcgtcag gtggatgttt ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg
    13621 gtgttttata tcagcaggat ctatgtgaac gctcttcagg acgggtcaac gcgtaaaaaa
    13681 aagccagcct gtttccagac tggcttttgt gcttttcaag ccggtgttac atcgcttttt
    13741 tggtcaactc gataacgcgc agctgcgcga tcgctttggc cagttccgca gacgcctgag
    13801 cgtaatctac gtcgccgtga gagctgctaa tgtgctcttc agccttacgt ttcgcttcca
    13861 tggctcgcgc ttcgtcgaga tcctggccgc gaattgcggt gtcggccaga acggtcacgt
    13921 tgccaggctg cacttcaaga atgccgccag acagatagat aaactcttcg tgaccgtgct
    13981 gtttcacgat gcgaatcata ccaggcttaa tggcggtgag cagcggtgcg tggccagggt
    14041 agatccccag ttcaccttcg ctacccgtta cctggatttt ctcgaccaga ccagagaaca
    14101 tttgttgctc tgcgctgacg acgtccaggt ggtaagtcat tgccatatca ccctccgatt
    14161 aaggcgttaa agttttttgg ctttttccac agcttcttcg atggaaccga ccatgtagaa
    14221 cgcctgctcc ggcaggtgat cgtattcgcc ttccatgatg cctttaaagc cacggatggt
    14281 gtctttcagg gagacgtatt tacccggaga accggtgaat acttctgcca cgaagaacgg
    14341 ctgggacagg aagcgctgga tcttacgagc acgcgctacc accagtttgt cttcttcaga
    14401 cagttcatcc atacccagga tggcgatgat gtctttcagt tcctgataac gttgcaggat
    14461 ggactgaacg ccacgcgcgg tgtcgtagtg ttcctgacca accaccagcg ggtccagctg
    14521 acggctggtg gagtccagcg ggtcaacggc cgggtagata cccagagacg cgatctgacg
    14581 gctcagtacc acggttgcgt caaggtgcgc aaaggtggtt gccggagacg ggtcagtcaa
    14641 gtcatccgca ggtacgtata ccgcctgtac ggaggtgata gaaccagttt tggtggaggt
    14701 gatacgttcc tgcagaacgc ccatctcttc cgccagggtc ggctgataac ctaccgctga
    14761 aggcatacgg cccagcagtg cggatacttc cgtaccggcc agggtgtaac gatagatgtt
    14821 gtcaacgaac agcagaacgt cacgaccttc gtcacggaat ttctcagcca tggtcagacc
    14881 ggtcagagca acgcgcagac ggtttcccgg cggctcgttc atctggccat acaccaggga
    14941 tactttgtcg ataacgttgg agtcggtcat ttcgtggtag aagtcgttac cctcacgagt
    15001 acgttcacct acgcccgcaa acacagagta accggagtgc tcgatcgcga tgttacgaat
    15061 gagctccatc atgtttacgg ttttacctac acccgcacca ccgaacagac caactttacc
    15121 gcccttagcg aacggacaca tcaggtcgat aactttgata ccggtttcca gcagttcctg
    15181 agagtttgac agctcttcgt aggaaggtgc tgcgcggtga atcgcccaac gctcttcttc
    15241 accgatctcg cctttcatgt cgaccggttc acccagtacg ttcatgatac ggcccagagt
    15301 cgctttacct accgggactt cgatcgggtg ttcgaggtct tttacatcca gaccgcgacg
    15361 cagaccgtcg gaggaaccca ttgcgatggt acgcacgata ccgccgccga gctgctgctg
    15421 aacttccagc accagacgct cattaccatt ttgcacctca agagcatcgt acacgcgcgg
    15481 tacggcatcc tgagggaatt cgacgtcaac tacggcgccg attacctgga caatctttcc
    15541 agtagccatc ttaaatcctc tacgaaataa cctgtttaaa ccgcggcggc ccccgagacg
    15601 atctcggtga gttcctgagt aatgctggcc tgacgagctt tgttgtatac caactgcagc
    15661 tctttaatca ggctgccgcc attgtcggtc gcggctttca tcgccaccat acgggcggcc
    15721 tgctcgctgg ccaggttttc aaccacgccc tgataaacct gagattcgac ataacgacgc
    15781 agcagggtat ccagcaacgc cttcggatcg ggttcgtaca ggtaatccca ggatttatgt
    15841 ttcagatcat catcatctga tgccggtaac ggcagcagct ggctgatggt cggaacctga
    15901 gacatggtgt taataaattt gttgctgaca atgtaaagct tgtccagacg gccttcgtcg
    15961 taggcctgca acatcacttt taccggaccg atcagttcgg acagggaagg gttatccccc
    16021 atgccggtga cctgggcaac aacattgccg cccacggagt tgaagaacga cacgcctttc
    16081 gagccgatca ttgcgaggtc gcattgaacg cctttgtcgg tccaggtctt catttccgcc
    16141 agcagttttt tgaacaggtt aatgttcaaa ccaccgcaca aaccacggtc ggtcgacacc
    16201 accaggtagc ccacgcgttt aacgtcgcgg tcttccaggt aagggtgctt atattccaga
    16261 ttaccgtgtg caaggtgacc aatcactttg cgcatggttt ctgcataagg acggctggcc
    16321 gccatgcgat cctgcgattt acgcattttg gaagcggcga ccatctccat cgctttagtg
    16381 atcttttgcg tgttctggac gcttgcgatc ttactacgta tctcttttgc gccggccatg
    16441 agcttctcct caatgccttg cggcctgccc taaggcaagc cgccagacgt taccaggatt
    16501 gggttgcttt gaaggaatcg aggatgcctt tcagcttgcc ttcgatttcg tcgttgtagc
    16561 caccggtctg gttgatctct tgcatcaacg gagcgtgatc acggtcgacg taagccagca
    16621 gagcggcttc gaagctgcca attttcgaca gttcaacatc cgccaggtaa ccacgttctg
    16681 ctgcgaacag aaccagagac tgctgcgcaa cggacatcgg cgcatactgt ttctgtttca
    16741 gcagttcggt cactttctga ccgtggtcaa gctgcttacg tgttgcatcg tcaaggtcgg
    16801 atgcaaactg agagaacgct gccagttcac gatactgtgc cagagcggta cggataccac
    16861 cggacagttt tttcatgatc ttggtctgtg ctgcaccacc aacacgggat acggaaatac
    16921 ccgggttaac cgcaggacga ataccggcgt tgaacaggtt ggtttccagg aagatctgac
    16981 catcggtaat ggagattacg ttggtcggaa cgaacgcaga aacgtcaccc gcctgagttt
    17041 cgataatcgg cagtgcggtc agagaaccgg ttttcccttt cacttcacct ttggtgaagg
    17101 cttcaacgta ttcggcgtta acacgtgcag cacgctccag cagacgagag tggaggtaga
    17161 aaacgtcgcc cgggaatgct tcacgtcctg gcggacgacg gagcagcagg gagatctgac
    17221 ggtaagcaac agcctgttta gacaggtcat cgtaaatgat cagcgcatct tcaccgcggt
    17281 cacggaagta ttcgcccatt gcgcaaccgg catacggtgc caggtattgc agtgcagcgg
    17341 attcagacgc ggttgctacc acaacgatgg tgttagccag tgcgccgtgc tcttccagtt
    17401 tacgtaccac gttagaaatg gtggacgctt tctggccgat agcgacatag atacatttga
    17461 taccggaatc gcgctggttg atgatggcat cgatagccag tgcggtttta cctgtctgac
    17521 ggtcaccgat gatcaattca cgctgaccac gaccgattgg gatcatggag tcaacggctt
    17581 tataaccggt ctgtaccggc tgatctacgg actgacgttc gataacaccc ggagcgattg
    17641 cttctacagc agagaagccg tcgtgatcca gcggaccttt accgtcgatt ggtgccccca
    17701 gagtgttaac cacacggccc agcaggccac ggccaaccgg aacttccagg atacggccag
    17761 tacacttaac tttcatgcct tcggcaaggt cagcgtacgg acccataaca accgcaccta
    17821 cagagtcgcg ctcgaggttc agtgcgatag cgtaacggtt acccggcagg gagatcattt
    17881 caccctgcat acaatcggcc aggccgtgaa tgcggataac accgtcactt acagaaacaa
    17941 tagtaccttc gttgtgagct tcactcacaa cattgaactg agcaatgcgc tgcttgatca
    18001 gttcgctgat ttcggtggaa ttcagttgca tgctccagtc cccttaagac tgcaagacgt
    18061 ctgcaaggcg ctcaagacga ccgcgtacgc tgccatcaat gaccatatca cccgctcgga
    18121 tgataacgcc tgccattaca gacttatcga ttttgcaatt cagcttaact ttgcgtgaca
    18181 gacgtttttc catcgcagca gaaattttcg cgagctgttg ttcactcagt gcggcagcgg
    18241 aaatgacgtc tacctcagcg gtagcctcac tcacggcacg aaggtgaata aactgctcca
    18301 gaacatccgg gagcgcgtta agacgaccat tttcagccat tacccgaatc aggttctgac
    18361 cgttttcgtc cagttgctca ccacaaactg cgataaacga ctcggcgagc gtttctggcg
    18421 caagcgcgcc agagagaagc tctgccattt gttcgttttt ggttacctcg gcggcaaacg
    18481 ccagcatgtc ctgccagcgt tctacacttt ggtgttcgac ggcaaagtca aaagctgctt
    18541 tggcgtaggg gcgagctacc gtaataaatt cagacatcag cccctccctc cttacagttc
    18601 agcgacaagt ttatccacga tgtcgctgtt agcagcttca tccacggaac gttcgatgat
    18661 cttctcggcg ccagcaacag ccaggatagc aacttgctta cgcagctctt cacgggcacg
    18721 tttacgctcg gcttcaattt ctgcctgcgc ctgggccacg attttagtac gttcctgttc
    18781 tgcctcagct ttcgcttcgt ccagaatctg cgagcggagt ttattcgcct gctcgatgat
    18841 tacctgggct tccgctttcg cttttttcag ctggtcggtc gcgctggcct ttgcaaggtc
    18901 aaggtcctta tgtgctcgtt ctgcggaagc aaggccgtca gcaatttctt tttgacgttt
    18961 ttcgatggct gccattaatg gcggccatac gtacttcatg cagaacagaa cgaacaggac
    19021 aaacgcgatg gcctggccga ggattgttgc gttaagattc acagcacaat gcctctattt
    19081 agttaacgtt ctgatattgc tctttaaata aaagcaacgc ttactacgcg acagcgaaca
    19141 tcacgtacag acccagacct acagcgatca tcgggatagc atccaccaga cccataacga
    19201 taaagaactg agtacgcagc agaggaatca gatcaggttg acgcgctgcg ccttccagga
    19261 atttaccccc gaggatgccg ataccgatcg cagcaccgat tgccgccaga cccatcatca
    19321 cagcggcagc catgtacagc agatccatat tcaggttttc catgacagtc tccagtttgt
    19381 ttcagttaaa acgtagtagt gttggtaaat taatgttctt cagacgccat cgacagatag
    19441 acgatcgtca gaaccatgaa gatgaaggct tgcagcgtaa tgatcaggat gtggaaaatg
    19501 gcccacggca cattcaggat ccactgtgac caccacggca acagaccagc aatcagaatg
    19561 aaaatcagct caccggcata catgttaccg aacagtcgca aaccgagtga aactggtttg
    19621 gacagcaggc ttaccccttc aaggattaag ttgacaggaa tgaacgccca gtgattgaac
    19681 ggctgcagcg tcaactcttt cgtgaagccg ccgatgcctt tcattttgat gttgtagaac
    19741 agaatcagga taaatacgcc cagtgccata gacagcgtta cgttcacgtc cgcagacgga
    19801 accacacgca gtgcaggcag acccagtaca tgttcagcaa tgtacggcag caagtcgata
    19861 ggcagtaaat ccatcagatt catcaggaat acccagacga agatcgtcag ggccagcgga
    19921 gcaatcagct tgcttttgcc atggtacatg tctttcacgc taccattaac aaagccgatc
    19981 accagctcaa tcgcggtctg aaacttacct ggcacaccgc tggtcgcctt tttggctacg
    20041 ctacggaata aaaccaagaa caacagaccc agcaccaccg agaagaacat ggagtcaata
    20101 ttgattgtcc agaaggtggc tggggggttt tgtggatcca ccagcgagaa tgtacgcagg
    20161 tccagctgaa ggttattcag gtggtgtcct atgtaatcct gcggcgtcat attttctgaa
    20221 gccatgatgc cttttaccct ttgttgttaa ttacagccgg tgccagtatc tgaaccacca
    20281 gcaccaaaac ccacgtaacg atcagcggca agaataccgc ctttaaaacc gccaacgcca
    20341 ccaccagtaa caccaacatc gccagaactt tgaaagcttc gccaaatgcg aatgtccaag
    20401 ccacccggcc tttcgctggt gtatgcgcct ggtgacgcca ggcaaatatc ataaacaaaa
    20461 cgttaggcag aaagactgcc aggcccccgc ttattgcaga gacgccccag aaggggtctt
    20521 tgaggctgaa cagcaatcca cttgctatca ccaccagtaa ctgaacgagc agaagcttcc
    20581 gagcaacgtt tcgactcacg agcgacacag acatcacgtt tttcactcct gctcccttcg
    20641 aggtatgccg cgtgtcgtat aaaactttct ttaaggctta gagtcaagca tcaaaaagcg
    20701 gtcaaattat acggtgcgcc cccgtgattt caaacaataa gtagccaaaa ggtgaataaa
    20761 tgtttaaata tttttccagt gcatacaatt gcgacttttc cgctacccct gttcgatcat
    20821 gaaaaactgt aaataacgcg taaacactgg tgataaagcg tgcttcagat cacatattgc
    20881 gcatgttcgc gcacagcata tttatttact tggcaaatga tgcctttgca agtttatgat
    20941 atttcagtct aaaaacagat actgttttaa taaatgacat ttacacaaca aaaaccaccc
    21001 attgacattt ttaataatgt tttaacagcc aatgatggtt cttagcgccg atttttagca
    21061 gactgatatt ttcactaatg acttattttc tgcttaccaa aaaaagccac gttatcttgt
    21121 tgatgcaaaa gagtgaacgt ggcgttaaat gtaaccagtt atatcagtag aaaacctggt
    21181 tgttgttaac agtctaaccg gtcaattttt tatgattttt ttgataaaaa ttaaatttta
    21241 tttgctttaa tcaccaccag atgacgttcg ccatccaggg ctggaacctg aagtttaacc
    21301 actgattcga cctgatattc ttcgggcaac aaagcgattt catcttccgg catttgccct
    21361 ttcagcgcgt agaaacggcc ttgctcacca ggaagatggt ggcaccagct caccatatcg
    21421 ttcagagagg caaaagcgcg gctaattacg ccatcaaatg gcggctctga aggaaactct
    21481 tctaccctgc tctgtactgg ttcaatattc tccagtttaa gctcatgttg cacctgacga
    21541 aggaaacgca cgcgtttacc aaggctatcc aacagagtga aatgggcttc aggacgcacg
    21601 atagagagtg gaatgcctgg cagtcctggt ccggtgccga catcgataaa ccgttcacct
    21661 tgcagatacg gtgccaccac aatgctatcg agaatatggc gtaccagcat ctcattagga
    21721 tcgcggaccg aagtcaggtt gtacgctttg ttccatttat gcagcatatt cacgtaggca
    21781 ataagctggt ttttctggtg atcagtaagc gaaatacctg cgtctttcag cagtaaggag
    21841 agtttgttga gcacggtgat tacctgttct tgatgcgttg cctggtaagc gggtgcttac
    21901 caggcatttt taatgcgtta tgcgctacga cgcagcatac cctgtttttt cagccacacc
    21961 agcagaatgg agatggccgc aggcgtgacg ccagaaatac gcgaagcctg gccgatagag
    22021 gccggtttgt gatcgttaag tttggcgatc acttcgttag aaagaccgga tacctggcgg
    22081 taatccagtg tcgcgggtag cagggtgttc tcgttacgca gctgcttttc gatctcatct
    22141 tgctggcgcg cgatataacc ttcgtattta acctgaatct caacctgttc cgccgcctgt
    22201 tcgtctgtca acgcaggggc aaacggcgtc agcgtggtta atttttcata agtcatttcc
    22261 ggacgacgca gcagatcttc accactggct tcacgggaaa gcggcgcagt caggtgagca
    22321 ttcacttcgg ctgcagcttc cgccgacggg gttacccagg tcgatttcag acgctgacgc
    22381 tcacgctcga tattctcaag tttctcgtta aagcgcgccc aacgttcgtc atccaccagg
    22441 cccagttcac gaccgatttc agtcaaacgc agatccgcat tatcttcgcg cagcatcaga
    22501 cgatattctg cgcgcgaagt aaacatacga tacggttctt tggttcctaa agtgcacagg
    22561 tcatcaacca gtacgccgag atacgcctga gaacgtgccg gagcccaacc ttctttgtca
    22621 gcagacagac gggcagcgtt aagaccggcc agcaaacctt gcgcagcggc ttcttcgtaa
    22681 ccggtagtgc cgttaatctg accagcaaag aacagcccct ggataaactt gctctccagc
    22741 gtcggtttca ggtcgcgagg atcgaagaag tcatactcaa tggcataacc cggacgcacg
    22801 atcttcgcgt tttccatccc ctgcatagag cggacgattt gcatctgcac atcgaacggc
    22861 aggctggtgg agataccgtt cggataaatt tcattagagg tcagtccttc cggttcaagg
    22921 aagatctgat gctgatttct gtctgcgaag cgcatgactt tgtcttcgat cgacgggcag
    22981 tagcgtgggc cgacaccttc gatcacccct gcatacattg ggctacgatc gaggttactg
    23041 cggatcacat catgggtttt ctcgttggta tgagtgatat aacacggcac ctgctgggga
    23101 tgcagggacg cattgcccat aaacgagaat accggcattg ggttatcgcc atgctgttgc
    23161 gccagcacgc taaagtcgat ggttcgagca tcaatacgcg gcggagtacc ggttttcaga
    23221 cgaccaacgc gcagcggcag ttcacgcaaa cggcgagaga gcggaatgga tggcggatcg
    23281 ccagcacggc caccgctgta attatccaga ccgatatgaa ttttgccgtc gaggaacgtc
    23341 ccaacggtga gcaccacagc tttggcacgg aacttcagtc ccatttgggt aacagcaccg
    23401 accacgcgat cgttttcgac aataagatct tcaaccgcct gctggaagat catcaggttc
    23461 ggttggttct ccagcgccgt acgtaccgcc tgacggtagc gcacacgatc cgcctgagct
    23521 cgggtagcgc gaaccgccgg tcctttgctt gcgtttagta tcctaaactg gatacccgct
    23581 tgatcgatcg ctttcgccat cagaccgccg agtgcatcca cttcttttac cagatgtccc
    23641 ttcccaatac cgccgatcgc cgggttgcag ctcatctgcc ccagagtgtc gatattgtgt
    23701 gtcaaaagca gagtctgttg acccatacgc gccgcagcca tcgcggcctc ggtgcctgca
    23761 tgacccccgc caatgatgat gacgtcaaaa ggatccggat aaaacatggt gattgcctcg
    23821 cataacgcgg tatgaaaatg gattgaagcc cgggccgtgg attctactca actttgtcgg
    23881 cttgagaaag acctgggatc ctgggtatta aaaagaagat ctatttattt agagatctgt
    23941 tctattgtga tctcttatta gaatcgcact gccctgtgga taacaaggat ccgactttta
    24001 agatcaacaa cctggaaagg atcattaact gtgaatgatc ggtgatcctg gaccgtataa
    24061 gctgggatca gaatgagggg ttatacacaa ctcaaaaact gaacaacggt tgttctttgg
    24121 ataactaccg gttgatccaa gcttcctgac agagttatcc acagtagatc gcacgatctg
    24181 tatacttatt tgagtaaatt aatccacgat cccagccatt cttctgccgg atcttccgga
    24241 atgtcgtgat caagaatgtt gatcttcagt gtttcgcctg tctgttttgc accggaattt
    24301 ttgagttctg cctcgagttt atcgatagcc ccacaaaagg tgtcatattc acgactgcca
    24361 ataccgattg cgccaaagcg gaccgcagaa agatcgggct tctgttcctg caatgcttca
    24421 tagaaaggag aaaggttgtc cggaatatct ccggcaccgt gggtggagct gataaccagc
    24481 cagatccccg aggcagataa atcttccaac agcggaccgt gcagcgtttc ggtggtaaaa
    24541 cccgcctctt ccagcttttc agccaggtgt tctgctacat attcggcacc gccgagggtg
    24601 ctgccgctga taagagtgat atctgccata aaccgccacc tttattaaga gtggcgtatt
    24661 gtacgctgtg aacgcgttgg gatctacctg tggaaaagta tgggattaaa aaagccgatc
    24721 agggcttgat ggtacgcatg atcgggtttt gcaggacgat caatgtctcg gtggactgaa
    24781 tttcatcaat tgtttggatc ttgttgataa gtacatgctg gagagcgtcg atcgaacggc
    24841 acataacttt tataaagatg ctgtagtggc cggttgtgta ataggcttca gtgacttcat
    24901 caaggctttc cagctttgcc agcgcggaag ggtagtcttt ggcgctcttt aatataatgc
    24961 cgataaagca gcctacgtca taaccgagct gcttcgggct gacatcaata cgcgccccgg
    25021 taatgatccc cgcctgcttc attttctcta ctcgaacgtg aatcgtcccc ggactgacgc
    25081 caaattgttt cgccagttcg gcgtaagcgg tgcgcgcatt gcccattaat gcttccagga
    25141 tgccacggtc cagattgtcg atcagataat tttccatagg attttcttat gcagattgat
    25201 gattcattct attttagcct tcttttttaa tgaatcaaaa gtgtgttagg ctttttattg
    25261 aatgattatt gcatgtgtgt cggtttttgt tgcttaatca taagcaacag gacgcaggag
    25321 tataaaaaat gaaaaccgct tacattgcca aacaacgcca aattagcttc gtgaaatctc
    25381 acttttctcg ccaactggaa gaacgtctgg ggctgatcga agtccaggcg ccgattctta
    25441 gccgtgtggg ggatggcacg caggataact tgtcgggctg tgaaaaagcg gtgcaggtaa
    25501 aagtgaaagc tctgcctgat gcccagttcg aagtggttca ttcactggcg aagtggaaac
    25561 gtcagacctt agggcaacac gacttcagcg cgggcgaagg gctgtacacg cacatgaaag
    25621 cccttcgccc cgatgaagac cgtctttctc cgttgcactc ggtctatgtt gaccagtggg
    25681 actgggaacg cgtaatgggc gacggtgagc gtcaattctc gactctgaaa agcacggtag
    25741 aggcgatctg ggcgggaatt aaagcaaccg aagctgcggt taacgaagag tttggcctgg
    25801 caccgttcct gccggatcag attcacttcg tacacagcca ggagttactg tctcgttatc
    25861 cggatctcga tgccaaaggg cgtgagcggg cgattgcgaa agatcttggc gcggtattcc
    25921 tcgtcgggat tggcggcaag ttgagtgatg gtcatcgtca cgacgtgcgc gcaccggatt
    25981 atgatgactg gagcaccccg tcagagctgg gccatgcggg cctgaacggc gatattctgg
    26041 tgtggaaccc ggtactggaa gatgcgtttg agctttcttc catggggatc cgtgtggatg
    26101 ccgacacgct gaagcatcag ctggcgctga ccggtgatga agatcgtctg cagctggaat
    26161 ggcatcaggc gctgctgcgc ggtgaaatgc cgcagaccat cggcggcggt atcggccagt
    26221 ctcgtttgac tatgctgctg ctgcaactgc cgcatatcgg ccaggttcag tgtggagtat
    26281 ggccagttgc tgttcgcgag agcgtccctt ctctgctgta ataatttatc gccgccagcg
    26341 tctgagcagg cggcttcgca tcccggtatc aaagcgccag atatgatcga aaatgcgcat
    26401 gatgccgggt ttgccgtgtg ccgacatcgc cacggcatga aagcgatgct gatgtacccg
    26461 ctgcagctct ttcactttac tcgtcacgtc gtcaggcaac cgctgagcga taaaatcaga
    26521 aatcaccacc gcatcggcat caaaccattc cctgctttgc aagcgttcca taatggcgcg
    26581 aaaacaactg gcaagatcgg tgccgccacg aaactgctgg cttaaaaaac ggattgcttg
    26641 ttcgatgcct tgtgggcctg aaagctcata acggacgatc tcggtggaaa atagcataat
    26701 ataacagcgc cggttttcag cgagagcaat gcgcatcaaa gccaggcaga acgctttcgc
    26761 acactgttca ttaaagccgc ccattgagcc ggaagtatcc acacagacaa taaacggccc
    26821 gcgcggctgt tcatcgtaat ctttatgtac caccggacgt tcgagcactt tttcacgcca
    26881 cgactcaccg tgcaggcgat aggtgagcaa ctgtttttcc accagccgac ggtaaaactc
    26941 atactccagt tccgttatcc ctagtgtcgc cagttctggc ggcaggagac gtaaaatatc
    27001 atcgctttgt tgcagaccat caacctgctc aggaaccgtc gccggttcgc gcaccatggt
    27061 gcggaaggtt tccatctacg catcgttgcg cggtattgat ttggcttccc gagaacgccc
    27121 cagctgctct gccaggcgtt tcagttccgg ctgttcgtta agaaattcac cgtatttcac
    27181 aataaactga tagtcgccac gtttaagctg accggcgctc atatcccaca gacgaccagc
    27241 tgcggtattg ttatctgcga gaatcggttc aagttgtccg ctcagcgtca tgcgttcctg
    27301 aacttcactt aacagttgtt cgcgttcttc ttctaatagc tgttgattta acgtcgttgc
    27361 ttgcacgatc agacttaaac gccaacgctg gagaaaaagc gtgtgtaacg ctgaagttat
    27421 tgtgctgtta gcatcaacca actgccgggc ttgttctgcc catggggaat tcagacgatg
    27481 cagtaagtct aggatctgtg gtagctgcac aataaactgc ggcgtggaga ggagctggct
    27541 ttgctgatag cacatcacct cttcggtgag ttccggcggg actcgggcat ctttcagccg
    27601 actgcgcagc gcctcacgcc agcggggaac atcatcagtg attgccgcct tcagtcgtgg
    27661 gaatttttca aagaagaccg ccagctgcgg tgaggccagc agtgcgatga tcatctcttc
    27721 gatcaatccc tcttcgctga cggccagcat cacattaagc gtatccagcg ttagcattgt
    27781 tgtgcctggc gaatctgtgc accgacatcc tgtaggctgg cttcaatgcg acctaaccag
    27841 tcgccaggga taaacagaca tttttgctgt tcgctgaata aagcgtgttg cttacgccag
    27901 tcgctttcaa gctcttccag ttgttgtttt atttcacctg gcagaccttc agccgatgaa
    27961 ccgggaagtg ccagcgtact gccttgtaaa cttacatcgc gtacaacaag atgttgggtg
    28021 ctatcaactt ccagattcag tttctgggca aagccgatac cgttcagttt gccgcgaatt
    28081 tcaccacctt tactcagcca ctgttccagc gcgctacgct caaaggagat atgaaccact
    28141 tccatatcat gcagttttaa cggtttttgc agcagcagag tcagagtgga agcagtaacg
    28201 ttaacaggaa gttgatactg ctgacgacgg ctgaaaatgc cgcccagacg aattaacgtt
    28261 aaggctgttt tatcgctttg ttgctgctgg agttgcaggt gacgttgcac aatcgcgccc
    28321 aggcgggtca acatcccttg ctgttgccag gcatgaccgg tcatcaatac atcaatttgt
    28381 tgttgtatca aattcaggct ttgcgcgtca taccacaggc aatctttcag caaaatgaga
    28441 tcaaccgggg caacagcact gcgaccgcta aaaaaggcgc tggcctgcaa taagcgccag
    28501 cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg cagcataaaa
    28561 atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg ccagcgttca
    28621 tattcttcat ctgtgacctg caaggcatca ggaaccggat tgtcgttttc atcctgttga
    28681 ctggtcagca tggagcggaa attcgcttta tcttgcactt tatctaacca tagacgaatc
    28741 agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc gttggaggcc
    28801 gccaccagta gacgcatcgg gattttttct acgtgtgcac cgttgcggaa ctggcgctcg
    28861 ttaatggcgg tgagcaaggt attaagaatt gccgggcccg ctttccagat ctcatccaga
    28921 aagacgattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg cccttcatct
    28981 tttagcgcct gaatagaaag gggaccaaaa acttcttccg gcgtggagaa gcgggtcatc
    29041 agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc gatcaaactt
    29101 ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc cgctaataaa
    29161 cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact gctcaggcgg
    29221 gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc tttcgtcaaa
    29281 agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata gtctcaaagg
    29341 ggggtaat
//
LOCUS       DADMFL010000044        29081 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.044, whole genome shotgun sequence.
ACCESSION   DADMFL010000044 DADMFL010000000
VERSION     DADMFL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.044"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="JNZ27_001924"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="JNZ27_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY8664275.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="JNZ27_001925"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="JNZ27_001925"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY8664276.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNZ27_001926"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JNZ27_001926"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY8664277.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNZ27_001927"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JNZ27_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY8664278.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNZ27_001928"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JNZ27_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY8664279.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /locus_tag="JNZ27_001929"
                     /pseudo
     CDS             complement(3421..4089)
                     /locus_tag="JNZ27_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="JNZ27_001930"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="JNZ27_001930"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY8664280.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="JNZ27_001931"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="JNZ27_001931"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY8664281.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="JNZ27_001932"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="JNZ27_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY8664282.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNZ27_001933"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JNZ27_001933"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY8664283.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNZ27_001934"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JNZ27_001934"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY8664284.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="JNZ27_001935"
     CDS             8207..9070
                     /locus_tag="JNZ27_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY8664285.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="JNZ27_001936"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="JNZ27_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY8664286.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="JNZ27_001937"
     CDS             10406..11023
                     /locus_tag="JNZ27_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY8664287.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTIIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="JNZ27_001938"
                     /pseudo
     CDS             complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="JNZ27_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            12960..13583
                     /gene="gmk"
                     /locus_tag="JNZ27_001939"
     CDS             12960..13583
                     /gene="gmk"
                     /locus_tag="JNZ27_001939"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY8664288.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTPPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13638..13913
                     /gene="rpoZ"
                     /locus_tag="JNZ27_001940"
     CDS             13638..13913
                     /gene="rpoZ"
                     /locus_tag="JNZ27_001940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY8664289.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13932..16040
                     /gene="spoT"
                     /locus_tag="JNZ27_001941"
     CDS             13932..16040
                     /gene="spoT"
                     /locus_tag="JNZ27_001941"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY8664290.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16047..16736
                     /gene="trmH"
                     /locus_tag="JNZ27_001942"
     CDS             16047..16736
                     /gene="trmH"
                     /locus_tag="JNZ27_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY8664291.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16742..18823
                     /gene="recG"
                     /locus_tag="JNZ27_001943"
     CDS             16742..18823
                     /gene="recG"
                     /locus_tag="JNZ27_001943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY8664292.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="JNZ27_001944"
     CDS             complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="JNZ27_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY8664293.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20342..21733
                     /gene="xanP"
                     /locus_tag="JNZ27_001945"
     CDS             20342..21733
                     /gene="xanP"
                     /locus_tag="JNZ27_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY8664294.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21854..23563
                     /locus_tag="JNZ27_001946"
     CDS             21854..23563
                     /locus_tag="JNZ27_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8664295.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="JNZ27_001947"
     CDS             complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="JNZ27_001947"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY8664296.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNAQTITL"
     gene            complement(25944..27327)
                     /locus_tag="JNZ27_001948"
                     /pseudo
     CDS             complement(25944..27327)
                     /locus_tag="JNZ27_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27620..27714
                     /locus_tag="JNZ27_001949"
     tRNA            27620..27714
                     /locus_tag="JNZ27_001949"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27654..27656,aa:Sec,seq:tca)
     gene            27986..29081
                     /locus_tag="JNZ27_001950"
                     /pseudo
     CDS             27986..29081
                     /locus_tag="JNZ27_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7181 a         7793 c         7308 g         6799 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacgcgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gccgctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccattgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc attatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccggcaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacggaatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctagc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 gttggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cgctacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccttccagt agtcatcgtc ccactgtttt atttgctgtt gcaggcggga
    12601 aatttcttcc tgtgctctgg ctggcgacca ggccggacag accgcccaca cagatgattg
    12661 ccagcacaag atacttatta atatcgccat ccatactttc atcttcacct ccgctgtagt
    12721 caggctggca gatatacaac gtggtgaagg caaagccgag tggcaaaaac ggagtctgcg
    12781 aggacgcttc ctgagaatcg tctttattgc agtaaatcac aggcaaatgc ggaaacagct
    12841 acgcaaaacg caacaacttt gcgcaaaaag tgtgagcaag ggctacgtca catggccgcg
    12901 ccgtgtataa taagctcgta tgtaggcttt atttcgctaa tcacatacga aagatactca
    12961 tggctcaagg cacgctttat attgtttctg cccccagtgg cgcgggtaaa tccagcctga
    13021 ttcaggcttt attaaaaacc ccaccgttgt atgacaccca ggtttctgtt tcacacacca
    13081 cacgccaacc gcgtcctggt gaagtccacg gtgaacatta tttctttgtt aatcatgatg
    13141 aatttaaaga aatgattagc agagatgcgt tcctcgaaca cgcagaagtt tttggtaatt
    13201 actatggcac ttcgcgtgag gccattgagc aagtactggc gaccggtgtc gatgtttttc
    13261 tcgatatcga ctggcagggc gcgcagcaaa ttcgccagaa gatgccgcac gcgcggagta
    13321 tctttatttt accgccgtcc aaaattgaac tggaccgccg tctacgcggt cgcggtcagg
    13381 acagcgaaga ggtcattgca aagcgtatgg cgcaagctgt tgcagaaatg agccattacg
    13441 ccgaatatga ttatctgatt gtgaatgatg acttcgatac cgcgttgacc gatttgaaga
    13501 ccattattcg cgccgaacgt ctgcgcatga gccgccaaaa gcagcgtcat gacgctttaa
    13561 tcagcaaatt gttggcagac tgaacctgat ttcagtatca tgcccagtca tttcttcacc
    13621 tgtggagctt tttaagtatg gcacgcgtaa ctgttcagga cgctgtagag aaaattggta
    13681 accgttttga cctggtactg gtcgccgcgc gtcgcgctcg tcagatgcag gtaggcggaa
    13741 aggatccgct ggtaccggaa gaaaacgata aaaccactgt aatcgcgctg cgcgaaatcg
    13801 aagaaggtct gatcaacaac cagatcctcg acgttcgcga acgccaggaa cagcaagagc
    13861 aggaagccgc tgaattacaa gccgttaccg ctattgctga aggtcgtcgt taatcacaaa
    13921 gcgggtcgcc cttgtatctg tttgaaagcc tgaatcaact gattcaaacc tacctgccgg
    13981 aagaccaaat caagcgtctg cggcaggcgt atctcgttgc acgtgatgct cacgaggggc
    14041 aaacacgttc aagcggtgaa ccctatatca cgcacccggt agcggttgcc tgcattctgg
    14101 ccgagatgaa actcgactat gaaacgctga tggcggcgct gctgcatgac gtgattgaag
    14161 atactcccgc cacctaccag gatatggaac agctttttgg taaaagcgtc gccgagctgg
    14221 tagagggggt gtcgaaactt gataaactca agttccgcga taagaaagag gcgcaggccg
    14281 aaaactttcg caagatgatt atggcgatgg tgcaggatat ccgcgtcatc ctcatcaaac
    14341 ttgccgaccg tacccacaac atgcgcacgc tgggctcact tcgcccggac aaacgtcgcc
    14401 gcatcgcccg tgaaactctc gaaatttaca gcccgctggc gcaccgttta ggtatccacc
    14461 acattaaaac cgaactcgaa gagctgggtt ttgaggcgct gtatcccaac cgttatcgcg
    14521 taatcaaaga agtggtgaaa gccgcgcgcg gcaaccgtaa agagatgatc caaaaaatcc
    14581 tctcggaaat cgaagggcgt ttgcaggaag cgggaattcc ctgccgcgtc agtggtcgcg
    14641 agaagcatct ttattcgatt tactgcaaaa tggtgctcaa agagcagcgt tttcactcaa
    14701 tcatggacat ctacgctttc cgcgtgatcg tcaatgattc cgacacctgt tatcgcgtgc
    14761 tcgggcaaat gcatagcctg tacaagccgc gtccgggtcg cgtgaaagac tacatcgcca
    14821 ttccaaaagc gaacggctat cagtctttgc acacctcgat gatcggcccg cacggtgtgc
    14881 cggttgaggt ccagatccgt accgaagata tggatcagat ggcggagatg ggtgttgccg
    14941 cgcactgggc ttataaagag cacggcgaaa ccagcaccac cgcgcaaatc cgcgcccagc
    15001 gctggatgca aagcctgctg gagctgcaac agagcgccgg tagttcgttt gaatttatcg
    15061 agagcgttaa atccgatctc ttcccggatg agatttacgt tttcacaccg gaagggcgaa
    15121 ttgtcgagct gcctgccggt gcaacgcccg tcgacttcgc ttatgcagtg cataccgata
    15181 tcggtcatgc ctgcgtgggc gcacgcgttg accgccagcc ttacccgctg tcgcagccgc
    15241 ttaccagcgg tcaaaccgtt gaaatcatta ccgcaccggg cgctcgcccg aatgccgcgt
    15301 ggctgaactt tgtcgttagc tcaaaagcgc gcgccaaaat tcgtcagttg ctgaaaaacc
    15361 tcaagcgtga tgattctgta agcctgggcc gtcgtctgct caaccatgct ttgggtggta
    15421 gccgtaagct gaatgaaatc ccgcaggaaa atattcagcg cgagctggat cgcatgaagc
    15481 tggcaacgct tgacgatctg ctggcagaaa tcggacttgg taacgcaatg agcgtggtgg
    15541 tcgcgaaaaa tctgcaacat ggggacgcct ccattccacc ggcaacccaa agccacggac
    15601 atctgcccat taaaggtgcc gatggcgtgc tgatcacctt tgcgaaatgc tgccgcccta
    15661 ttcctggcga cccgattatc gcccacgtca gccccggtaa aggtctggtg atccaccatg
    15721 aatcctgccg taatatccgt ggctaccaga aagagccaga gaagtttatg gctgtggaat
    15781 gggataaaga gacggcgcag gagttcatca ccgaaatcaa ggtggagatg ttcaatcatc
    15841 agggcgcgct ggcaaacctg acggcggcaa ttaacaccac gacttcgaat attcaaagtt
    15901 tgaatacgga agagaaagat ggtcgcgtct acagcgcctt tattcgtctg accgctcgtg
    15961 accgtgtgca tctggcgaat atcatgcgca aaatccgcgt gatgccagac gtgattaaag
    16021 tcacccgaaa ccgaaattaa tgttttatga acccaacacg ttatgcacgc atctgcgaaa
    16081 tgctcgccag gcggcagcct gatctgaccg tctgcatgga gcaggtccac aaacctcata
    16141 acgtttctgc gattattcgt accgcagatg ccgttggcgt acatgaagtt cacgccgtct
    16201 ggcctggtag ccgcatgcgc accatggctt cggcagcggc gggtagtaac agctgggtac
    16261 aggtgaaaac acaccgcacc attggcgatg ccgtcgctca tctcaaaggc cagggcatgc
    16321 agattctggc aacccatctt tctgataatg ctgtcgattt ccgcgaaatt gattacactc
    16381 gcccgacctg cattttgatg ggacaggaga aaacgggcat cacgcaggaa gcattggccc
    16441 tggcggatca ggacatcatc attccgatga tcggcatggt gcagtcgctg aatgtttccg
    16501 ttgcctcagc cctcattctt tacgaagccc agcgtcagcg gcaaaatgca ggcatgtacc
    16561 tgcgtgaaaa cagcatgttg ccggaagcag agcaacaacg cctgttgttt gaaggcggct
    16621 atccggtgct ggcgaaagtc gcaaaacgca aaggcctgcc ttatccccac gtcaatcagc
    16681 aaggcgagat cgaagctgat gccgactggt gggctactat gcaggctgca gggtaagtgc
    16741 catgaaaggt cgcctgttag atgctgtccc actcagttcc ctaacgggcg ttggcgcagc
    16801 acttagtaac aaactggcga aaatcaacct gcataccgtg caggatctac tcttacacct
    16861 tcccctgcgc tacgaagatc gcacccatct ctaccccatc ggagaactac tgccgggcgt
    16921 ttatgccacg gtggaaggcg aagtgctgaa ctgcaatatc tccttcggcg gtcggcggat
    16981 gatgacctgc cagatcagcg acggttccgg catcctcacc atgcgctttt tcaatttcag
    17041 cgcggcaatg aaaaatagcc tggcgacggg ccgccgtgta ctggcttatg gcgaagcaaa
    17101 gcgcggtaaa tatggtgcgg agatgatcca cccggaatac cgcgtgcagg gcgatctcag
    17161 cacgccagaa ttacaggaaa cgctcacgcc ggtttatcca acaacggaag gcgtaaagca
    17221 ggccacgctg cgtaaattaa ccgaccaggc gctggatctg ctcgacacct gcgccattga
    17281 agaactcctg ccgccggaac tgtcacaagg aatgatgacg ctaccggaag cgttgcgcac
    17341 tttgcaccgc ccgccaccga cgctacagct tagcgatctg gaaaccgggc agcatccggc
    17401 gcaacgtcgt ctgattctgg aagaactgct ggcgcacaac ctcagcatgt tagccttacg
    17461 tgccggagca cagcgttttc atgcccagcc gctgagcgcc aatgacacgc tgaaaaataa
    17521 actcctcgcc gccttaccgt tcaagccaac gggcgcacag gcacgcgtag tggcggagat
    17581 cgagcatgat atggcgctgg atgtgccgat gatgcgcctg gtacagggcg atgtaggttc
    17641 cggtaaaacg ctggtcgccg ccctcgccgc gctgcgtgcg attgcccacg gcaaacaggt
    17701 ggcactgatg gcaccaaccg aattactcgc cgagcagcac gccaataact tccgcaactg
    17761 gtttgcaccg ctcggtatcg aagtgggctg gctcgccggt aagcagaaag gcaaagcgcg
    17821 actgtctcag caggaagcca tcgccagcgg tcaggtgcag atgattgtcg gtacacacgc
    17881 catcttccag gaacaggtgc agtttaacgg cctggcgctg gtgattatcg acgaacagca
    17941 tcgttttggc gtgcatcagc gtctggcatt gtgggagaaa ggccagcagc agggcttcca
    18001 tccgcatcag ttgatcatga ccgccacgcc gatcccccgc acgctggcaa tgacggcgta
    18061 tgccgatctc gatacctcgg tgatagatga gctgccgcca ggccgcacgc cagtgactac
    18121 ggtcgctatt cctgataccc gccgtaccga catcattgac cgcgtgcgcc acgcctgcat
    18181 aactgaaggt cgtcaggcgt actgggtttg tacgttgatt gaagagtcag agttactgga
    18241 agcgcaggcg gcggaagcta cctgggaaga gttgaaactg gcgctaccag aattgaatgt
    18301 tggcctggta cacgggcgga tgaaaccagc cgagaaacag gcggtgatgg cgtcgtttaa
    18361 acaaggtgag ctacatctgc tggttgccac taccgttatt gaagtcggtg ttgatgtgcc
    18421 taacgccagc ctgatgatta tcgaaaaccc tgagcgtctg ggtctggcgc agttacacca
    18481 gctgcgtggg cgcgtaggtc gtggcgcggt ggcttctcac tgcgtgctgc tctacaaaac
    18541 gccgctttct aaaacggcgc aaattcgcct gcaagtgctg cgcgacagta acgacggttt
    18601 tgtgattgcg caaaaagatc tggagattcg cggccctggc gaattgttag gcacgcgtca
    18661 gacgggtaat gctgaattta aagtggcgga tttactgcgc gatcaggcga tgatcccgga
    18721 agttcagcgc ctggcgcgac atattcacga acgttaccca caacaggcaa aagccttgat
    18781 agaacgctgg atgccggaga cagaacgtta ctcgaatgcg taaaaggcgg cggtgagaag
    18841 accgccgctt catcggttaa ccggcaaaaa tcggcaacat taaatacagc ttaatcacca
    18901 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    18961 gcgacgggcc aaagcgttca gtgatcgcct gcatgttggc gattgccgtt ggcgttgcac
    19021 cgaggccaaa accacagtga cccgcagcca gcactgccgc atcgtagttt ttgcccatca
    19081 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    19141 ccagcatcgg cagtgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    19201 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    19261 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    19321 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    19381 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    19441 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    19501 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    19561 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    19621 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    19681 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    19741 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    19801 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    19861 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    19921 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    19981 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    20041 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    20101 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    20161 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    20221 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    20281 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    20341 aatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    20401 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    20461 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    20521 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    20581 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    20641 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    20701 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    20761 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    20821 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    20881 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    20941 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    21001 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    21061 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    21121 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    21181 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    21241 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    21301 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    21361 gatccagttg actggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    21421 cgtgctgggt ctgttcccgg cagtgagcga ttttgtacaa cacattccag aaccggttct
    21481 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    21541 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    21601 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    21661 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    21721 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctctttc
    21781 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    21841 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    21901 agtggtgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    21961 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg atcaccgttt
    22021 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gtcagcccgc
    22081 gcccctggtg gcaaaaagtg tcgacattgc gctaagcagt cgccaactga ccgaaccacg
    22141 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    22201 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    22261 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    22321 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    22381 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    22441 gactaacctg ggtgccgaca tcgcccgcgg gacattaacc ggaaacgcgc agcgtaacgc
    22501 cgacggcagc tggcaagtgg aaaatctgcg catggcggat atccgtctac aaagcgaaaa
    22561 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgctt
    22621 ggaagtcatc gatgctcgtc tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    22681 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caggaaggca aactgtcgat
    22741 gaacgccagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    22801 attttccccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    22861 cagaacgtca gggaactggc tgcgtgacgg taaaacgttg atccttgatg atgcggcaat
    22921 tgccgggctg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    22981 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    23041 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    23101 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    23161 cgcgacattc aatcgtgttg atgttcgtcg cccgtcgctg gcgctgaccg ccaacagcag
    23221 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    23281 aagtgtttca caaacgccac aacgtcagac ccatatcagc ctgaatggac gcggtgtgcc
    23341 ggtgaatatt ttgcaacaat ggggatggcc tgaattacag ttgactggcg acggcaatat
    23401 tcagcttacc gccagtggag atattcaggc caatgccccg ctgaaaccta cggttagcgg
    23461 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcat
    23521 cgtttccagc ggtgaagtta catcgacgga gccggtgcgg taaacatcaa tctcgccggg
    23581 tacgactcag acgtacccgg cattccgtca atagattaca acgtaattgt ctgcgcattc
    23641 ccttgaggcg tcaccaccag cccctgctca ctttcagcct gcgaaccgcc ttgcagacca
    23701 tttactttca caacattgcg caagcacagc gtccagttct tcgcctcgcc cgcaccagtc
    23761 acagtaatcg tgttgccagt acgtgctgct tttaaagtaa agatcaccga tccgtcagca
    23821 gcaggcactt cacagacggc ttcatgcccg tcttgcagat taaagaggtg gaatgccgtg
    23881 ccttcgtgcc acacgtaatc gggacgttga tcgttgttgc ccagcgccag tagagtgtta
    23941 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactgccg
    24001 tcgagttcat cgttgtgcca caggtgtgtc cagcgacctt ccggcaggta gaactgcaca
    24061 tcgcccgctt cagtgaacac cggcgcaacc atcacgttgt cgcctaacat gtattgacgg
    24121 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    24181 gtaccccgcg cgttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    24241 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    24301 taagatttgc taccgtgtaa acggctatgg ctggagagca aaccaaacgc gcaccagcgt
    24361 ttgtaaacgt gcgccggagc ggtattttca aagccgccga tatcgtggct ccagaagcca
    24421 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    24481 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    24541 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    24601 ttgtagatgt acgcataatg gttatgcatt ttctgcggat cggaaccgtc aaaccactga
    24661 acatcagttg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    24721 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    24781 gccagacctg gctgccattt atcccactgc catagcgaac cgtccgggcg tttgagtaaa
    24841 tagcctttct cttgtaactc tttaaagacg ggggattttt gaccgatata ggggttaatc
    24901 cagacgcaga ttttcagccc tttcgctttc aggcggcgga tcatcccttc cgggtcaggg
    24961 aaagtcagcg ggtcccactc aaaatcgcac cactggaagg ctttcatcca gaaacagtca
    25021 aagtggaaaa catgcagcgg cagattgcgt tccgccatac catcgataaa gctgtttacc
    25081 gtcgcttcgt cgtagttggt ggtaaatgaa gtggttagcc acaggccgaa ggaccacggg
    25141 ggcggcagcg ccggacgacc agtaaagcgg gtataacgat caagtaccgc tttcggcgtc
    25201 gggccgtcga taacaaagta ttcgagatat tcactctcaa cgctgaactg cactttggag
    25261 actttctccg atcccacttc aaaagagaca cactggggat gattgaccag taccccataa
    25321 ccacggttag tcatgtagaa cgggatattt ttatacgcct gttcagtact tgtgccgccg
    25381 tcccggttcc aggtctctac cgtctggcca ttgcgcacca gggcagtaaa gcgctctccc
    25441 agaccgtaaa ctgtttcgcc aacgccaaga tcaagccgct caaacatata attgcgttga
    25501 ttattcgtgt cctgcacgta gccattattt ttcacctgac taccggtaat acgttcgccg
    25561 ttgcgcagaa aatccagtga ccagaactca cctttgctga cacgcgcgct taagttgcca
    25621 cttttaaact cagcataacg ttctgtgttt tcgattgtga ccttcacgtc ctgcaaaata
    25681 ttgagcggat aatgaggacc gttattcagc gccccctgaa aatactcaat ccgcacaccg
    25741 acaatacctt cctgtgggga gaaaaagcgc agcgtaaata aaggcgtatc aagctgccag
    25801 gtacgttcac gcacatcacg gggggcagca tagaccacca tttcattatc ctgctgttca
    25861 acctcgaata cctgaagcgg gtgaatcaaa ttgaggccag gttgaatcaa ccagtttcca
    25921 tcgctaattt tcattgccgt tccttagttc tgcacttctt gagaggtgaa ttgttgctgg
    25981 caacggcgtt taccctgagc cagctgttcc ataacggttt tcagattgtg cgtcgtgagt
    26041 gagtagtagc gtttagcgat aatcgcgctc agcaaataac agatcgccgg aacaatcgtg
    26101 aatagcgcaa taatgatgct aatcgtggcg ctgttctgcg ctttttctgc cgcatcatat
    26161 ccgccataag ccagcatcca gccgataaga gcgccgccga aggccaaccc cagtttgagc
    26221 acaaacagcg tgccagcaaa actgatcccg gtcaggcgtt taccattgca ccactcgccg
    26281 tagtcgacgg tatcgaacat cattacccac tggataggtg tcaccagttg atgcaacaca
    26341 ccaatcacga agatgaagac aaacatagtg atgctggcct gcatgggaac aaagaacatc
    26401 gcgaggctaa tcactgccag cagggcgttc gtccaccaga agatagtgac tttacatttc
    26461 cagtcggtca gaggttttgc cagtgcggaa ccaatcaggt tacccacgca ataagtggtg
    26521 agaaaagcga caaacacttc cggcgtgccc aaaatccatg tgacgtaata catcatcgcc
    26581 ccaccgcgta cgcacaccgc caggatattg aaaatggtta gtaaaccgac aatccgccac
    26641 tggtcgtttt gccagatatc acgtaaatct tcccgcatag acgttgttgt aggtggtgct
    26701 tcaacgcgtt ctttagtggt gaagaaacaa aatgccagca tcatgaatgc caccacggaa
    26761 aggaccacga taccgccctg gaaaccgagt ggtttattat caccgccaat taaattaacc
    26821 agtggcatca tcagaacagt agaaagcatg cctcccgcgg tcgccagcac aaaacgccag
    26881 gattgcagcg agatacgctg agtcgggtca ttggtgatta caccacccaa tgcgcagtaa
    26941 gggatattga cgacggtata aagtaaggta agttagggtg taagtaattg ctgcatagat
    27001 cattttgccg ttcatactga gatctggcgt gctataggcc agtacacaga cgatcccgaa
    27061 tggcagtgcg ccaaacagta cccacggacg aaatttaccc cagcgagagc gcgttcgatc
    27121 ggccaacaac cccatgcaag gatcggaaat cgcatccagt gaacgagcga ccaaaaacat
    27181 ggttccgaca aatccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa
    27241 cattacgtta tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc
    27301 tttaacggac aacacttcac tcttcatctg cttttccttg atgctatagg ggtgctgaga
    27361 ccgataacgc attttgtaaa ccatccctgt gagagcgaat attccgatat ctggttatta
    27421 atttatgatt cttgttttat gtgatcgtgg tagcgttaat tccgctcata tatcattgta
    27481 aaatatgggt tttatataaa ttataatgct ttcgtgataa tacgctgcgt ttattaggcg
    27541 gaaaaaactg atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc
    27601 catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc
    27661 cagttggggc cgccagcggt cccgggcagg ttcgactcct gtgatcttcc gccagtttac
    27721 ctcttctgaa ctctcccaat gtcaataaat ctcttctaat cccttgtata tcagtattta
    27781 tttgtatcct gaggtcaacc tacttcaccc cgtatcaagt ttcttttggg ggtactttag
    27841 ggggtgttat ttcctattga aatacaagca cccccataat aggaaaaatg tgatacataa
    27901 caacggcttt taacaatatc tttgttatac aaggggtgta ttgggggtat caggttagtt
    27961 actggcagaa atccccctca ggaacatgaa ttcatcaaaa gccggaggat gtcatggcat
    28021 taaccgatat caaagtgaaa accgcaaaac ccaaagacaa accctataag cttgctgatg
    28081 gcggcggtat gtatctactg attaacacta atggttctaa atactggcgc atgaagtacc
    28141 gctttgctgg taaagaaaag atgctttcta ttggcgtata ccccgatgta acgctagccg
    28201 atgctcgcga gaaacgcagt gaagccagga aactcctcgc tgctggaggc gatccagggg
    28261 aggcaaaaaa ggaagaaaaa atcgcgcagc aaatgagtct gaaaaatacg ttcgaggcta
    28321 ttgctcgcga atggcaccag ttaaaagctg atcggtggtc attacgctat cgtgatgaaa
    28381 tcattgatac ctttgaaaaa gacatttttc cctacattgg caagcgccct attgccgaaa
    28441 tcaaaccaat ggagttactg gaagcactgc gtaaaatgga aaaacgtgga gctctggaga
    28501 aaatgcgcaa ggtccgccag cgctgtggtg aggtgttccg ctatgcaatt gttactggcc
    28561 gggcagacta taacccagcc cccgatcttg ccagtgcttt agctacaccg aaaaaagtac
    28621 attttccctt tcttactgct aatgaacttc cccacttcct caacgatctg gcgggttata
    28681 ccggaagtat aatcactaag acagctactc agatcattat gttaaccggc gtacgaacac
    28741 aagaactgcg ttttgcacgc tgggaagata tcgattttga gacaaaattg tgggagatcc
    28801 cggcagaagt aatgaagatg aagcgacctc atattgtgcc gctctctgag caggtcatta
    28861 tgctatttaa gcagcttgaa ccaatctcaa aacatcatcc cctagtcttt atcggtagaa
    28921 atgatccccg caaaccaatt agtaaggaaa gcattaacca agtaattgaa ttacttggat
    28981 ataagggaag actcacaggg cacggtttca gacacaccat gagtacaatt ctgcatgaac
    29041 aaggctttaa ttccgcctgg attgaaatgc agttagctca t
//
LOCUS       DADMFL010000045        28748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.045, whole genome shotgun sequence.
ACCESSION   DADMFL010000045 DADMFL010000000
VERSION     DADMFL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.045"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            353..1711
                     /locus_tag="JNZ27_001951"
     CDS             353..1711
                     /locus_tag="JNZ27_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAY8664297.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="JNZ27_001952"
     CDS             complement(1950..3335)
                     /locus_tag="JNZ27_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8664298.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JNZ27_001953"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JNZ27_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8664299.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="JNZ27_001954"
     CDS             complement(3729..4109)
                     /locus_tag="JNZ27_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY8664300.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="JNZ27_001955"
     CDS             complement(4464..4898)
                     /locus_tag="JNZ27_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664301.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="JNZ27_001956"
     CDS             complement(4886..5287)
                     /locus_tag="JNZ27_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664302.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="JNZ27_001957"
     CDS             complement(5547..6116)
                     /locus_tag="JNZ27_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY8664303.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7037)
                     /locus_tag="JNZ27_001958"
     CDS             complement(6861..7037)
                     /locus_tag="JNZ27_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAY8664304.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
     gene            complement(7338..7604)
                     /locus_tag="JNZ27_001959"
     CDS             complement(7338..7604)
                     /locus_tag="JNZ27_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664305.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="JNZ27_001960"
     CDS             7745..7951
                     /locus_tag="JNZ27_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8664306.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="JNZ27_001961"
     CDS             8046..8648
                     /locus_tag="JNZ27_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664307.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9848..10765
                     /locus_tag="JNZ27_001962"
     CDS             9848..10765
                     /locus_tag="JNZ27_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664308.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="JNZ27_001963"
     CDS             10850..11722
                     /locus_tag="JNZ27_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY8664309.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12094..14940
                     /gene="ag43"
                     /locus_tag="JNZ27_001964"
     CDS             12094..14940
                     /gene="ag43"
                     /locus_tag="JNZ27_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAY8664310.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            15011..15169
                     /locus_tag="JNZ27_001965"
     CDS             15011..15169
                     /locus_tag="JNZ27_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY8664311.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            15324..16142
                     /locus_tag="JNZ27_001966"
     CDS             15324..16142
                     /locus_tag="JNZ27_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY8664312.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            16484..16957
                     /locus_tag="JNZ27_001967"
     CDS             16484..16957
                     /locus_tag="JNZ27_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY8664313.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            16973..17449
                     /locus_tag="JNZ27_001968"
     CDS             16973..17449
                     /locus_tag="JNZ27_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY8664314.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            17512..17733
                     /locus_tag="JNZ27_001969"
     CDS             17512..17733
                     /locus_tag="JNZ27_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY8664315.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            17896..18270
                     /locus_tag="JNZ27_001970"
     CDS             17896..18270
                     /locus_tag="JNZ27_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY8664316.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            18317..18691
                     /locus_tag="JNZ27_001971"
     CDS             18317..18691
                     /locus_tag="JNZ27_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY8664317.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            18688..19179
                     /locus_tag="JNZ27_001972"
     CDS             18688..19179
                     /locus_tag="JNZ27_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664318.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            19191..19388
                     /locus_tag="JNZ27_001973"
     CDS             19191..19388
                     /locus_tag="JNZ27_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY8664319.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            19473..20033
                     /locus_tag="JNZ27_001974"
                     /pseudo
     CDS             19473..20033
                     /locus_tag="JNZ27_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            20105..21109
                     /locus_tag="JNZ27_001975"
     CDS             20105..21109
                     /locus_tag="JNZ27_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HAY8664320.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            21391..21672
                     /locus_tag="JNZ27_001976"
                     /pseudo
     CDS             21391..21672
                     /locus_tag="JNZ27_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(21822..21909)
                     /locus_tag="JNZ27_001977"
     tRNA            complement(21822..21909)
                     /locus_tag="JNZ27_001977"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21873..21875),aa:Ser,seq:gga)
     gene            22143..23081
                     /gene="ghrA"
                     /locus_tag="JNZ27_001978"
                     /pseudo
     CDS             22143..23081
                     /gene="ghrA"
                     /locus_tag="JNZ27_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            23136..23873
                     /locus_tag="JNZ27_001979"
     CDS             23136..23873
                     /locus_tag="JNZ27_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY8664321.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            23897..24451
                     /locus_tag="JNZ27_001980"
     CDS             23897..24451
                     /locus_tag="JNZ27_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8664322.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            24553..25044
                     /locus_tag="JNZ27_001981"
     CDS             24553..25044
                     /locus_tag="JNZ27_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY8664323.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(25108..25883)
                     /locus_tag="JNZ27_001982"
                     /pseudo
     CDS             complement(25108..25883)
                     /locus_tag="JNZ27_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(25946..26326)
                     /gene="csgF"
                     /locus_tag="JNZ27_001983"
     CDS             complement(25946..26326)
                     /gene="csgF"
                     /locus_tag="JNZ27_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY8664324.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTI"
     gene            complement(26351..26740)
                     /gene="csgE"
                     /locus_tag="JNZ27_001984"
     CDS             complement(26351..26740)
                     /gene="csgE"
                     /locus_tag="JNZ27_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY8664325.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(26745..27395)
                     /gene="csgD"
                     /locus_tag="JNZ27_001985"
     CDS             complement(26745..27395)
                     /gene="csgD"
                     /locus_tag="JNZ27_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY8664326.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            28149..28604
                     /gene="csgB"
                     /locus_tag="JNZ27_001986"
     CDS             28149..28604
                     /gene="csgB"
                     /locus_tag="JNZ27_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY8664327.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            28645..>28748
                     /locus_tag="JNZ27_001987"
     CDS             28645..>28748
                     /locus_tag="JNZ27_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY8664328.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         7120 a         7257 c         7336 g         7035 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggtgt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgagctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt acggttctgc gtgccgtccg tgacacggtg gtctctgttg cccgcgctgt
    11701 atggaactgg attttcttct gaacagtctg ccagggaaat gcctgataac attacgttaa
    11761 ccaactgaaa acatgacaca aacccctcat actcctcctg tttctcttgc gtgaccgcct
    11821 tgtcgttaat agaataaaac tatcgataaa acagatatcg atcgttgcta tcgatcgttt
    11881 atagtggtta atggtctgcg aaaaggcagc tgaatctctt catcatgcag aacggaattg
    11941 cacacaacgg actgatactt ctctggcgga tgagagaggg gaagcattta tgtccgggaa
    12001 aaaccacttt atctgaccat gctgttttgt acctgccggt atccactttt gtgggtaccg
    12061 gcttttttat tcaccctctg taaggaaaag ctgatgaaac gacatctgaa caccagctac
    12121 aggctggtat ggaatcacat tacgggcacc ctggtggtgg cttccgaact ggcccgctca
    12181 cggggaaaac gcgccggtgt ggcggttgtg ctgtctcttg ctgctgtcac atcagtcccg
    12241 gcactggctg ctgacacgat tgtacaggcg ggagaaaccg tgaacggcgg aacactgaca
    12301 aatcatgaca accagattgt tctcggtacg gccaacggaa tgaccatcag tagcggtctg
    12361 gagtatgggc cggataacga ggccaatacc ggcgggcaat ggatacaaaa tggcggtatc
    12421 gccaacaaca ctactgtcac cggtggtggt cttcagagag tgaatgccgg aggaagcgtt
    12481 tcagacacgg ttatcagtgc cggaggcgga cagagccttc aggggcaggc agtgaatacc
    12541 actctgaacg gcggtgagca gtgggtacat gaaggcggga ttgcaacggg taccgtcatt
    12601 aatgagaagg gctggcagac cgtcaaatcc ggcgcaatgg caaccgacac ggttgtgaat
    12661 accggcgcgg aagggggacc agatgcagaa aatggtgata ccgggcagtt tgttcgcgga
    12721 aatgccgtac gtaccactat caatgaaaat ggtcgtcaga ttgtggctgc tgaaggaaca
    12781 gcaaatacca ctgtggttta tgccggcggc gaccagacgg tacacgggca tgcgctggat
    12841 accacactga atggcggtta ccagtatgtg cacaacggcg gtacggcgtc tggcactgtt
    12901 gtaaacagtg acggctggca gattatcaag gaaggtggtc tggcggattt caccaccgtt
    12961 aaccagaaag gcaaactgca ggtgaacgcc ggtggtaccg ccacacatgt caccctgaaa
    13021 cagggtggag cactggttac cagtactgcg gcaacggttc ttggcagcaa ccgtctgggc
    13081 aatttcacgg tggaaaacgg taaggctgac ggagttgttc tggagtccgg cggtcgcctg
    13141 gatgtactgg aaggccattc agcgcagaaa acacgggtgg atgacggcgg aaccctggca
    13201 gtgtctgccg gtggtaaggc aacaggtgtc accatgacat ccggtggtgc cctgattgca
    13261 gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat tgatggcaca
    13321 tccggtcagg ccagcggact gctgctggaa aatggcggca gctttacggt taatgccgga
    13381 ggactggcca gcaacaccac tgtcggacat cgtggaacac tgacgctggc tgccggggga
    13441 agtctgagtg gcagaacaca gctcagtaaa ggtgccagca tggtactgaa tggcgatgtg
    13501 gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca gacgacaccg
    13561 gatgccgcgc tgagccgtgc tgttgcaaaa ggcgactccc cggtaacgtt ccataaactg
    13621 accaccagta acctcaccgg tcagggtggc accatcaata tgcgtgttcg ccttgatggc
    13681 agcaatacct ctgaccagct ggtgattaat ggtggtcagg caaccggcaa aacctggctt
    13741 gcgtttacaa atgtcggaaa cagtaacctc ggggtggcaa cctccggaca gggtatccgg
    13801 gttgtggatg cacagaatgg cgccaccaca gaagaaggtg cgtttgccct gagtcgcccg
    13861 cttcaggccg gcgcctttaa ctacaccctg aaccgtgaca gcgatgaaga ctggtacctg
    13921 cgcagtgaaa atgcttatcg tgctgaagtc cccctgtata catccatgct gacacaggca
    13981 atggactatg accggattct ggcaggctcc cgcagccatc agaccggtgt aaacggtgaa
    14041 aataacagcg tccgtctcag cattcagggc ggtcatctcg gtcacgataa caacggcggt
    14101 attgcccgtg gagccacgcc ggaaagcagc ggcagctatg gcttcgtccg tctggagggt
    14161 gacctgctga gaacagaggt tgccggtatg tctctgacga caggggtgta tggtgccgca
    14221 ggccattctt ccgttgatgt taaggatgat gacggttccc gcgccggcac ggtccgggat
    14281 gatgccggca gcctgggcgg atacctgaat ctgacacaca cgtcctccgg cctgtgggct
    14341 gacattgtgg cacagggaac ccgccacagc atgaaagcgt catcggacaa taacgacttc
    14401 cgtgcccggg gctggggctg gctgggctca ctggaaaccg gtctgccctt cagtatcact
    14461 gacaacctga tgctggagcc acaactgcac tatacctggc agggacttac cctggatgac
    14521 ggccaggata acgccggtta tgtgaagttc gggcatggca gtgcacaaca tgtgcgtgcc
    14581 ggtttccgtc tgggcagcca caacgatatg agctttggtg aaggcacctc atcccgtgac
    14641 actctgcgcg acagtacaaa acaccgtgtg agtgaactgc cggtgaactg gtgggtacag
    14701 ccttctgtta tccgcacttt cagctcccgg ggtgacatga gcatggggac agccgccgcc
    14761 ggcagtaaca tgacgttctc accgtcccgg aatggcacgt cactggacct gcaggccgga
    14821 ctggaagccc gtgtccggga aaatatcacc ctgggcgttc aggccggtta tgcccacagc
    14881 gtcagcggca gcagcgctga aggttataac ggtcaggcca cgctgaatgt gactttttga
    14941 taatcaggtc actctccttc aggccccggt catcatgacc ggggcttttt actttcaggg
    15001 agcaacagat atgccgggtt gtacatcacg tttattgccc gaaggccctt tctcacgcga
    15061 acaggctgtt gccgtcaaaa cagcttaccg caatgtgttt actgaagatg accagggaac
    15121 atattcccgg ctggttatcc gtaatgctga aggtcagttg cgctggtgat gccggaacac
    15181 agcttaatac tcagctcgcc agttaagggc ttctcagaca ataaacgtca ttcatttcat
    15241 ctgtcaggcc gcgtcttctc cgggagacgc agctttttca tttattacac cagtaaatcg
    15301 taaccaccga taaggagcaa attatgcgac tagccagtcg ttttggttat gcgaaccaga
    15361 tacgccgtga ccgtccgctg acacacgaag aactgatgca ctatgttccc agtattttcg
    15421 gtgaagaccg gcatacttcc cgcagtaaac gatatgcgta cattcccacc atcaccgtac
    15481 tggaaagcct gcagcaggaa ggttttcagc cattcttcgc ctgccagacc cgcgtgcgcg
    15541 acccgggccg ccggggatac acaaaacaca tgctgcgtct gcggcgggcc ggagagataa
    15601 acggagaaca tgtccctgaa attattctgc tcaactctca tgacggtacc tccagctacc
    15661 agatgctgcc gggttacttc agattcgtct gccagaatgg ctgcgtctgc ggtcagtctc
    15721 tgggggaagt gcgtgttcca caccggggaa atgtggtgga gaaagttatc gaaggggctt
    15781 acgaagtggt gggggttttt gaccggattg aggaaaagcg tgatgccatg cagtcgctgg
    15841 tcctgccgcc accggcacgc caggcgctgg cacaggcggc actgacttac cgttatggtg
    15901 acgaacatca gccagtcacc accgctgaca ttctgacacc acgacgcagg gaggattacg
    15961 gtaaagacct gtggagcgca tatcagacca tccaggagaa tatgctgaaa ggcgggattt
    16021 ccggccgcag tgcaaaagga aaacgtatcc acacccgtgc cattcacaac atcgacaccg
    16081 atattaagct caaccgcgca ttgtgggtaa tggcagaaac gctgctggag agcctgcgct
    16141 gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg ccccggaggc
    16201 gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta cccgtctgct
    16261 ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg catctggcca
    16321 tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc gaatatcatt
    16381 catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc cagctcccga
    16441 cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa cattgtctca
    16501 gaacaccacc gcttcggcct gtgcaccgga gactggcctg caacaactgg ttgccactcc
    16561 cgtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca ttccacagtg
    16621 ggtcaccctg gaaccccgtg tcttcggctg gatggaccgt ctgtgtgaag actactgcgg
    16681 tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggcac ccgaaccgga
    16741 tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc gcgctgaaat
    16801 gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc atgcctgtcg
    16861 tacggagtgt tatgccatga cggtccatta ttaccggctg cgggattacg ccctgcagca
    16921 tccggaatgc agcgccatta tgcgcatcat cgactgaatg gaggcaggga caatgcagca
    16981 gctttccttt ctgcccggaa acatgacgcc cggcgagcgc agtctcattc tgcgggccct
    17041 gcaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca cccgtgcggc
    17101 acgggaatgg ctgattctga acatggcggg actggagcgg gaagagttcc gggtgctgta
    17161 tctgaacaac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca ccatcaaccg
    17221 tacggaagtt catccccggg aagtgattaa acgcgccctg taccacaatg ccgctgccgt
    17281 ggtgctggcg cacaatcacc cgtccggtga agtcacaccc agtaaggcag accggcttat
    17341 caccgaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat
    17401 agtcggtggc aaccaggttt tctcctttgc cgaacatggt ctgctttaac ccgtcacaac
    17461 cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacat catgagaatc
    17521 atcacccgtg gtgaagccat gcgtattcac caacagcatc ctgcatcccg tctttttccg
    17581 ttctgtaccg gtaagtatcg ctggcacggc agtgctgaag cgtatactgg tcgtgaagtg
    17641 caggatattc ccggcgttct tgccgtgttt gcagaacgcc gtaaggacag ttttggcccg
    17701 tatgtccggc tgatgagtgt caccctgaac tgatgttccg tcacccggga aacgagtacg
    17761 accagatgac ctcagacgcc gctgtggatg aggccattct catcaatgca tacatcttca
    17821 ccgaaaccgg tcagcgcgcc ggctgacctg tcatcgttaa accattctta accacttttc
    17881 agagaggatt ttatcgtgtc agacaaactc tccgggataa ctcatcccga tgacaatcac
    17941 gaccgcccct ggtgggggct tccctgcaca gtgaggccct gttttggcgc tcgtctggtg
    18001 caagagggta accgcctgta ttaccttgcc gaccgcgccg gtatcagagg ccgcttcagc
    18061 gacgcagatg cataccatct ggaccaggcc tttccgctgc tgatgaaaca actggaactc
    18121 atgctcacca gcggtgaact gaatccccga tatcagcata ccgtcacgct gtatgcaaaa
    18181 gggctgacct gcgaagccga caccctcggc tcctgtggtt acgtttatct ggctgtttat
    18241 ccgacaccag cacccgcaac cacctcataa ccccgaagct ctcaccttta gcactaccag
    18301 ttacagagaa cttaagatga aaacattatc cgacacacat gtacgggagg tatctcgctg
    18361 cccgtctccc gtcaccatct ggcagacact gctcatccga ctgctggacc agcattatgg
    18421 cctcacactg aatgacacac cgtttgtcga tgaacgtgtg attgagcagc atattgaggc
    18481 aggcatttca ctgtgtgatg cagttaattt tctcgtggaa aaatacgcgc tggtgcgtac
    18541 cgaccagccg ggattcagca cctgtccccg ctctcagtta ataaacagta ttgatatcct
    18601 ccgggctcgc cgggcgaccg gcctgatgac ccgcgacaat tacagaacgg taaataacat
    18661 taccctgggt aagtatccgg aggcgaaatg atgaaactgg ccctgacact ggaagccgac
    18721 agcgttaacg tacaggcact gaacataggg cgcattgtcg ttgacgtcga tggtattgag
    18781 ctcgctgaac tgattaacat ggtctgcgat aacggctact cccttcgtgt tgttgatgaa
    18841 tctgaccaga cctcagcaga atgcacgcca ccatttgcta cccttaccgg catacgctgc
    18901 agtaccgcac atatcacgga aacggacaac gcctggttgt actcgctgtc acaccagacc
    18961 agtgacttcg gtgaatcaga atggattcat tttacaggta gcggatatct gttacgtacc
    19021 gatgcgtggt catacccggt tctgcggctt aaacgcctgg ggctgtcaaa aacgttccgt
    19081 cgtctggtcg tcacactcat ccggcgttat ggcgtcagtc tcattcatct ggatgccagc
    19141 gctgaatgcc tgccgggttt acccactttc gactggtaac caggaacaac atgaaatcat
    19201 taaccacgga aacagcgctg gatattctga ttgcgtggct gcaggacaat atcgactgcg
    19261 gatcgggaat tatctttgac aacgatgagg ataaaacgga ttcagcagca ctgttgccct
    19321 gtatcgaaca ggcccgggag gatgtccgta ccctgcgtca tctgcagctt ctgcaccaga
    19381 accggtgaag ctcactcacc agacttcatt cctctgacgc cagcctgcac agggctggcg
    19441 ttttcattta tctgcaaaaa ggaatatcga ttatgtcaga tatcacaatc tcccgtccgg
    19501 aagtggtcaa cgggcatacg gacgttatct gctcaaccag cattcgccac attctggctg
    19561 tacgaaagag tacactgttg caaatcgaca cacttatccg acaactggct gaaatctcgg
    19621 tgctgacgga aagcatcgga ggtaaaactg ccccggactg ggccatgaaa caggattttc
    19681 gctgcggctg ctggctgatg gagaaacccg aaacagcaat gaaagccatt acccggaatc
    19741 ttgatcgcga aatctggcgt gacctgatgc aacgttccgg gatgctgtca ttaatggata
    19801 cgcaggcccg tgatacatgg taccggtcac tggagtacga taattttccg gaaatcagcg
    19861 aagcgaacat tctgagcaca tttgaacaac tgcaccagaa taaggatgag gtgtttgagc
    19921 gaggggtgat caacgtcttc agagggctga actggaatta caaaaccaat tgcccctgta
    19981 aatttggcag taaaattatc gtcaacaatc tggtgagatg ggaccggtgg ggataatgat
    20041 atggtcaccc ccaccttaac agcagtacgc tgttaaggta aatcccttta acggagagac
    20101 catcatgcag aacatcacac ttattggtat cgatctcgga aagcactcct tccacattca
    20161 ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa tttacccgta ctaaactgat
    20221 gcagtttctg gcgacatgtc cgccctcagt tgttgtaatg gaagcctgtg cgggtgcaca
    20281 ctttatggct cgctgtatct ctgattttgg gcatgaaact aagctgattt ctccacagtt
    20341 cgttcgcccg tttgtcaaaa gtaataagaa tgattttgtt gatgcagaag ccatatgcga
    20401 agcggcttca cgaccatcaa tgcgttttgt ccagccaaga actgagaccc agcaggccat
    20461 gagggcgtta caccgggtca gagaatcgct catcagagat aaggtgaaaa caaccaacca
    20521 gatccacggt ttcttgctgg aatttggaat cagcctgccg ataggtgatg ctgtaataaa
    20581 acggttgtct ctcgtgttgg ctgaacacga aatccccgaa tatctgagcc gccttttggt
    20641 gaggttgcat acccactact tttaccttgt tgagcaaata gcagagctgg aatcagaatt
    20701 aagtcagtca atcaacgctg atgataccgc tcagcgcatt atgacaattc ctggtgttgg
    20761 tccgattact gccagcttac tatcgtcgca acttggtgac gggaaacagt tctcatgcag
    20821 cagggatttt gcagcttcca caggacttgt tccccggcag tatagcactg gaggaaaatc
    20881 gactctgttg ggtatcagta agcgaggtga taaaaattta cgccggttac tggttcagtg
    20941 tgcccgctca ttcataatgc gacttgaacg caagcatgga aaacttgctg agtgggttcg
    21001 ggagcaactc aacaagaagc attcgaatgt ggttacctgc gctctggcaa ataaactggc
    21061 tcgaattgcc tgggcaatca ctactcagaa aaaagaatat caggcctgat atagatgctg
    21121 atataactca attagtttat tttaaacaat aagatacaag ttacctttct ggttttgcga
    21181 agactgatta ttgatgacat gaacggcaaa tcgacctgat taaaaaccta acagatgaaa
    21241 tggctcactg aagccgaggg gatttttagg attatcaggt gcggaactca tcggggcgcg
    21301 ggcatccttg cccacagaga cgccggatag atttaagcaa gccaaccatt aatcaaaaat
    21361 cagtgttgca aaaacggggg tgaccataga tttcatctta tcaccggaca acaggctgac
    21421 cgactcgccg atctggaaag aatgttgcac ctgttcagcg gtaaaccaat ccccgacaac
    21481 cggggaaaca tcacccttcg tctggatgat cacattcagt ctgttcaggg taaagagagc
    21541 tatgaagatg agatgttcag catcagatac tttaagaagg gctccgcgca tatcacgttc
    21601 agaaaaccag aactggttga cagactgaat gatattatcg caaaacacta tcctgatatg
    21661 ctggcagtat agctttgcca gtatgctcaa agatcggaaa ttgtccgata agatatgatc
    21721 gcggggcata taaaccggtt tcagatggca tacccgatgg catactgtct ggaaggaaaa
    21781 ataaaaaagc gcatgttaaa caataacatg cacttataat ctggcggtga gggggggatt
    21841 cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga
    21901 cacctcacca aattgtcgtt cctgtcttgc tggaacgggc gctaatttag ggaaatcatg
    21961 acctgaggtc aacaaacttt ttgaaagaat cgcgcgttta ttcaaacttc aatcaatgtg
    22021 tggttttaat aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg
    22081 gcctttgtag atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca
    22141 gtatggatat catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc
    22201 gcaaagctat tcctcaggca agagtcaggg catggaaaag cggagataat gactctgctg
    22261 attatgtttt agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg
    22321 tgttcgcact cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa
    22381 tgctgaaccc ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc
    22441 aggaatatgc tgtcagtcag gtgctgcatt ggtttcgacg ttttgatgat tatcgcatcc
    22501 agcaaaatag ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg
    22561 gcattttggg cgcaggcgta ctgggctgta aagttgctca gagtctgcaa acctagcgct
    22621 ttccgctgcg ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg
    22681 gacgggaaga actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga
    22741 atacccctga aaccgtcggc attattaatc aacaattact cgaaaaatta cctgatggcg
    22801 cgtatctcct caacctggtg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc
    22861 tggatagcgg gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc
    22921 ctgaaagtcc gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta
    22981 cccgtcccgc tgaagctgtg gagtacattt ctcgcactat tgcccagctc gaaaaagggg
    23041 agagggtctg cgggcaagtc gaccgcgcac gcggctacta ataaagcatc aggattcctg
    23101 ctatccttgg cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc
    23161 ataccgttgc cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac
    23221 aaaagggcat taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc
    23281 atcactggca cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc
    23341 tgcgcggcat tgaagctaac attaaaaatg ttgatggtga aattgactgc agcggtaaaa
    23401 tgtttgactc gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg
    23461 acaaagcgac caatacacaa gcgatgatcg ccactatcgc cagcggcaat gtgcatataa
    23521 taagccatcc cggaaatccg aaatatgaaa tagatgttaa agccgttgct gaagcagccg
    23581 cgaaacatca ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca
    23641 gtgaagacaa ctgtcgtgcg gtagctgcag cggtacgtga tgctggaggt tgggtggcat
    23701 taggctcgga ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc
    23761 tcgacgcggt agattttccg ccagagcgca ttttgaatgt ttctcctcgc cgcttactga
    23821 acttccttga atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag
    23881 taatggaaat atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta
    23941 ttaccgccaa ccgcaagatc ctttactggt gccgctgttt actctgattc gtgaggggaa
    24001 actggctgcg aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg
    24061 tgatatgacg caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt
    24121 gccgccatat cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct
    24181 ttccgaacga gggatgccac tggcggatac gccagccgat cacatcggca cattgctgct
    24241 tgcggcttcc tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact
    24301 gttcagtgag tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc
    24361 aaccacgcct ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg
    24421 ggacgagctg gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac
    24481 agataatttg ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct
    24541 atcatgcgcg gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt
    24601 atctggggat gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt
    24661 acggcctact ttgcttgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc
    24721 ctgcttagtg gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat
    24781 ctggaaattc tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc
    24841 aaacagctgc tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc
    24901 gggcagggtg actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac
    24961 gcaatgaaaa acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag
    25021 caggaaatca aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaatgccg
    25081 gattattaat tccggcattt ttatctgtca ggattccggt ggaaccgaca tatggcggta
    25141 tttcaccaga atgtcattct gccgttctgc tttattttgc aaatcccaca gaccacggtc
    25201 gataccatca ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat
    25261 aacgggttcg tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa
    25321 gcggaaaacc ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga
    25381 gaggatctca ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg
    25441 gtattgcgtg tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt
    25501 gctttcataa ccaataatcg aaccttcaac cataatattt gccgccgtta aagattgcag
    25561 cgggattcgg ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taaccttgcg
    25621 ctcgttaagc aggttttgta agccctggcg ctttgcggaa cagcagtgga gaagttactt
    25681 gccgggtagg gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc
    25741 ttacccgtcg gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat
    25801 gtcggtctgg cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca
    25861 accaaaagaa ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg
    25921 ttgagttatt ttgtaaaccc gaaacctaga tggtcgaggt ttgtccggtt ttacgatctg
    25981 tcacgttcaa ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca
    26041 ccatgcggcc cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact
    26101 ggatggcctg agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat
    26161 agctcggatc tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat
    26221 ttgggttacc accaaagttt ggattacgga actggaaagt catggttcca gcccaactta
    26281 atggcgaaat aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac
    26341 aattttttat ttagaattca tcatgcgcca aatcgcccgt acttaataac gcctgattta
    26401 tctggcgacg atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcga
    26461 agtctctttt caacgggaat aaaaaagtct ggaaaataac atcctgattg accgttattg
    26521 tgatccagct tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt
    26581 cactttccca tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat
    26641 ggtcagttaa caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca
    26701 gaaattctgc cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta
    26761 tcgtttgccc aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta
    26821 taaagatgcg ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta
    26881 gacgcgccga tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat
    26941 tccgtgctgt tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc
    27001 gtaaagtagc attcgccacg caggacgcct tgcaacccat tgacaacacg ttcttgatcc
    27061 tccatggcat aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa
    27121 tcttcaggcg tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc
    27181 tgccaataat ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct
    27241 gaagagatat cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgctagcgat
    27301 tgtttaaggt gctgcaagag agctgtcgcc tgcaaagaag gtttagtgat caacaataat
    27361 gtatgaccat gaatactatg gacttcatta aacatgatga aaccccgctt ttttattgat
    27421 cgcacacctg acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact
    27481 gctgtgtgta gtaataaatc agccctaaat gggtaaaata taaaactaat ggattacatc
    27541 tgatttcaat ctagccatta caaatcttaa atcaagtgtt aaacatgtaa caaaatgtag
    27601 ctcattatat taaaatgtta atattaaggt tttattaagt ttagaaatga tagaaaagtt
    27661 gtacatttgg tttttattgc acaattttaa aaaatcatac aaatggtgct aacttactaa
    27721 taatacatat aaaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt
    27781 tctccacacc tccgtggaca atttttttac tgcaaaaaga cgaggtttgt cacggcttgt
    27841 gcgcaagaca tatcgcagca atcagcgacg ggcaagaaga atgactgtct ggtgattttt
    27901 gatagcggaa aacggagatt taagagaaaa caaaatattt tttgcgtaga taacagcgta
    27961 tttacgcggg ttttaatact ttggtatgaa caaaaaaaga aaaatacaac gcgcgggtga
    28021 gttattaaaa atatttccgc agacatactt tccatcgtaa cgcagcgtta acaaaataca
    28081 ggttgcgtta acaaccaagt tgaaatgatt taatttctta aatgtacgac caggtccagg
    28141 gtgacaacat gaaaaacaaa ttgttattta tgatgttaac aatactgggt gcgcctggga
    28201 ttgcagccgc agcaggttat gatttagcta attcagaata taacttcgcg gtaaatgaat
    28261 tgagtaagtc ttcatttaat caggcagcca taattggtca agctgggact aataatagtg
    28321 ctcagttacg gcagggaggc tcaaaacttt tggcggttgt tgcgcaagaa ggtagtagca
    28381 atcgggcaaa gattgaccag acaggagatt ataaccttgc atatattgat caggcgggca
    28441 gtgccaacga tgccagtatt tcgcaaggtg cttatggtaa tactgcgatg attatccaga
    28501 aaggttctgg taataaagca aatattacac agtatggtac tcaaaaaacg gcaattgtag
    28561 tgcagagaca gtcgcaaatg gcaattcgcg tgacacaacg ttaattacca ttcgactttt
    28621 aaatcaatcc gatgggggtt ttacatgaaa cttttaaaag tagcagcaat tgcagcaatc
    28681 gtattctccg gtagcgctct ggcaggtgtt gttcctcagt acggcggcgg cggtaaccac
    28741 ggtggtgg
//
LOCUS       DADMFL010000046        28594 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.046, whole genome shotgun sequence.
ACCESSION   DADMFL010000046 DADMFL010000000
VERSION     DADMFL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.046"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            243..1136
                     /locus_tag="JNZ27_001988"
     CDS             243..1136
                     /locus_tag="JNZ27_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8664329.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(1133..1947)
                     /locus_tag="JNZ27_001989"
                     /pseudo
     CDS             complement(1133..1947)
                     /locus_tag="JNZ27_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(1947..3563)
                     /locus_tag="JNZ27_001990"
     CDS             complement(1947..3563)
                     /locus_tag="JNZ27_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY8664330.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3858..4532
                     /locus_tag="JNZ27_001991"
     CDS             3858..4532
                     /locus_tag="JNZ27_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8664331.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(4571..6232)
                     /locus_tag="JNZ27_001992"
     CDS             complement(4571..6232)
                     /locus_tag="JNZ27_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY8664332.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6428..6856)
                     /gene="ibpB"
                     /locus_tag="JNZ27_001993"
     CDS             complement(6428..6856)
                     /gene="ibpB"
                     /locus_tag="JNZ27_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY8664333.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6968..7381)
                     /gene="ibpA"
                     /locus_tag="JNZ27_001994"
     CDS             complement(6968..7381)
                     /gene="ibpA"
                     /locus_tag="JNZ27_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY8664334.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7686..8018
                     /locus_tag="JNZ27_001995"
     CDS             7686..8018
                     /locus_tag="JNZ27_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY8664335.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8020..9193)
                     /locus_tag="JNZ27_001996"
                     /pseudo
     CDS             complement(8020..9193)
                     /locus_tag="JNZ27_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            9258..10276
                     /locus_tag="JNZ27_001997"
                     /pseudo
     CDS             9258..10276
                     /locus_tag="JNZ27_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(10237..11574)
                     /locus_tag="JNZ27_001998"
     CDS             complement(10237..11574)
                     /locus_tag="JNZ27_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664336.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11649..12797)
                     /gene="dgoD"
                     /locus_tag="JNZ27_001999"
                     /pseudo
     CDS             complement(11649..12797)
                     /gene="dgoD"
                     /locus_tag="JNZ27_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12794..13411)
                     /locus_tag="JNZ27_002000"
     CDS             complement(12794..13411)
                     /locus_tag="JNZ27_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY8664337.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQAMKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13395..14273)
                     /locus_tag="JNZ27_002001"
     CDS             complement(13395..14273)
                     /locus_tag="JNZ27_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY8664338.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14270..14959)
                     /gene="dgoR"
                     /locus_tag="JNZ27_002002"
     CDS             complement(14270..14959)
                     /gene="dgoR"
                     /locus_tag="JNZ27_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY8664339.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15237..15893
                     /locus_tag="JNZ27_002003"
     CDS             15237..15893
                     /locus_tag="JNZ27_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664340.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15939..16751)
                     /gene="yidA"
                     /locus_tag="JNZ27_002004"
     CDS             complement(15939..16751)
                     /gene="yidA"
                     /locus_tag="JNZ27_002004"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY8664341.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16865..17272)
                     /locus_tag="JNZ27_002005"
     CDS             complement(16865..17272)
                     /locus_tag="JNZ27_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY8664342.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(17503..19917)
                     /gene="gyrB"
                     /locus_tag="JNZ27_002006"
     CDS             complement(17503..19917)
                     /gene="gyrB"
                     /locus_tag="JNZ27_002006"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY8664343.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19946..21019)
                     /gene="recF"
                     /locus_tag="JNZ27_002007"
     CDS             complement(19946..21019)
                     /gene="recF"
                     /locus_tag="JNZ27_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY8664344.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(21019..22119)
                     /gene="dnaN"
                     /locus_tag="JNZ27_002008"
     CDS             complement(21019..22119)
                     /gene="dnaN"
                     /locus_tag="JNZ27_002008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY8664345.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLQQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22124..23527)
                     /gene="dnaA"
                     /locus_tag="JNZ27_002009"
     CDS             complement(22124..23527)
                     /gene="dnaA"
                     /locus_tag="JNZ27_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY8664346.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23824..23904
                     /locus_tag="JNZ27_002010"
     CDS             23824..23904
                     /locus_tag="JNZ27_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664347.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            24134..24274
                     /gene="rpmH"
                     /locus_tag="JNZ27_002011"
     CDS             24134..24274
                     /gene="rpmH"
                     /locus_tag="JNZ27_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY8664348.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24291..24650
                     /gene="rnpA"
                     /locus_tag="JNZ27_002012"
     CDS             24291..24650
                     /gene="rnpA"
                     /locus_tag="JNZ27_002012"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY8664349.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24614..24871
                     /gene="yidD"
                     /locus_tag="JNZ27_002013"
     CDS             24614..24871
                     /gene="yidD"
                     /locus_tag="JNZ27_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY8664350.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24874..26520
                     /gene="yidC"
                     /locus_tag="JNZ27_002014"
     CDS             24874..26520
                     /gene="yidC"
                     /locus_tag="JNZ27_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY8664351.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26626..27990
                     /gene="mnmE"
                     /locus_tag="JNZ27_002015"
     CDS             26626..27990
                     /gene="mnmE"
                     /locus_tag="JNZ27_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY8664352.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            28232..28306
                     /gene="tnaC"
                     /locus_tag="JNZ27_002016"
     CDS             28232..28306
                     /gene="tnaC"
                     /locus_tag="JNZ27_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY8664353.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
BASE COUNT         7015 a         7619 c         7189 g         6771 t
ORIGIN      
        1 tgcgaacctt ccactaggtg atcaccgcca ccagcagcgt aaaaccgaca aaactcaaga
       61 tttgtaacga attcatctca tagcccttat ttatgtttat gtcccggacg catgaatata
      121 gggagaggta aaatgtgtcg ggtcaggcag tcagattctt aaaatacaaa cgtcgtatcc
      181 ctgaacggat tgttttctgt taagttcagg ttgtgagcat aattcgccgg agggatatta
      241 aaatgaatgg aaaattgcaa agttcagatg taaaaaacga aactccgtac aatattccct
      301 tattgattaa cgaaaatgtg atctccagcg gaatttctct gatctcgctc tggcatacct
      361 acgccgacga gcattaccgg gtgatctggc cgcgggacaa gaagaaaccg cttatcgcca
      421 actcatgggt tgcggtgtat accgtacaag gatgtgggaa aattctttta aagaatggcg
      481 aacaaataac tctgcatggc aactgtatta tatttttaaa gccaatggat attcactctt
      541 atcactgtga aggtttagtc ttggaacagt actggatgga atttaccccc accagtatga
      601 tggatattcc cgttggtcag caaagcgtta tttataatgg cgaaatttat aatcaggaac
      661 tcaccgaagt tgctgagtta ataacttcac cagaagcaat aaaaaataat ctggcagtcg
      721 cttttctgac gaaaattatt tatcagtgga tttgtcttat gtccgcagat ggtaaaaaag
      781 atccacaacg gcggcaaatt gaaaaattaa ttgccacttt acatgccagt ctgcaacaac
      841 gctggagcgt agctgatatg gctgccacga tcccctgtag cgaagcctgg ttgcgtcgtc
      901 tgtttttacg ctataccggc aagacgccga aagaatatta cctcgatgcg cgtctggatc
      961 tggcgctatc gctattaaag caacaaggaa actcggttgg cgaagtcgct gatacgctca
     1021 acttcttcga ctcctttcat ttcagcaaag cctttaaaca taaatttggt tatgcgccgt
     1081 cagccgtgct aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta
     1141 actctggcca gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg
     1201 aggcgctcgg taccgttttc gacaacgtaa tcgcctgcca caggtgctga tatgaacgct
     1261 gttcccaggc gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct
     1321 gaaactgcgg gatatcgccg accaccagtg gttcaggccg tagtagcgca cgcgcatctg
     1381 tttgcgatct tgcagcccaa caatttgcgc catccggctt tcaataccga ttggcatgtc
     1441 acagatgttg aggattttcg cattcgggcg cagacgacgc gtggcttctg cgacaattgc
     1501 tgccgggttg gagtagttga gcatctaggc gtttggtgaa tatttttcca tataatccac
     1561 cagttccagg acgccgccaa tggaacgcat gccgtacgct attccgccgg gtccgcaagt
     1621 ttcctgacca acaacaccgt ggcgcagtgg gattttttca tccagttcgc gcatcgggta
     1681 cttgccgacg cggatgtgcg ccatgacaaa atcaacgtcg ctgaacgcca cttcaggatc
     1741 ggtggtgtaa ctaaacgcaa tgtccggcgc tttttcttta aggatgactt tacaggcttc
     1801 ggcaatcact tcctgccgcg cgccatcgtt atcataaaat ttcagtgcac gaagcgggaa
     1861 acggtcctga ttcgccagga gcatcaacac gatgcctggc gtaaaggtgc ttccaccgcc
     1921 tgcgacaacc actgagaatt tggtcattat actgcctccg taatggcaac attttctgct
     1981 gattgatgag aattaattaa gctatcgagc tgttcacgca attgggatac atgcagacca
     2041 atgatcacct gaatggcatc gccactacgg aagacgccgt gcgctcccag ctttttaaag
     2101 acttcgtcat ccagcgtttg tgacatgtca tgcagtgcaa tacgtagacg cgtcgcgcaa
     2161 ttgttaatgc tggagatatt gccgaccccg cccagggctt gcaggatacc ggcagcctga
     2221 tccagctctt tttttggctc tgcagcggtg gtttggcctc gcgaggcttt gtattcggct
     2281 tttgagtaga gtttcacttc cgcatcttca cgtcccggcg tgcacatgtt gaactgcaga
     2341 atcagggtgc ggaaaaccac gaagtacagc agggtaaagc acaacccaat ggcgatttgg
     2401 gtcagcatca tatccgcgtg gttgctgaac atcgggatcc agttttgcgg taaaacctgg
     2461 tcaatcagac ctccgcccat gttgcccacc acaccaaaga gatacattac ggtcgacatt
     2521 gaggccgcca gcacggcgtg taccgcaaac agcaacggtg aaatgaacag gaaggtaaat
     2581 tccagcggtt cggtaattcc caccagcatg gcggttaagg ttgcgggaat cagcaagccc
     2641 gccactttta cccgattttc cggtgcggca gtgaagtaca tcgctaaaga aatgcccacg
     2701 gcaccaaaga tttttgagtt accgtgcagg gcaaaaccgc cttccgggaa caacgatttc
     2761 agcggctcgg cgctcagact gaactcttgc agatgctgcg cccagtacat ctgaatgccg
     2821 ccttcaacag ctgccggacc aaagataaac ggtccgtaga tgaagtggtg taaaccggtt
     2881 gggatcagaa tacgttcgag gaaggtgtaa acccacaccc caagcgcacc cgccgaacgc
     2941 aggaacgctt gcagagattc aatccccatt tgtacttttg gccagccgag caatgtcagc
     3001 caggcacagg ggatcatcac caggaaggcg ataatcacca cataagacgt cccctggaaa
     3061 atgccgagaa aaaccggcag ttttttatcg aacagacggt tatgcagcgc cgtcacaatg
     3121 ccggaaatga taattgcgcc gataatgctg gtatcgaggg ttttaatccc ggccatcatt
     3181 gtcagaccgc tacctgccac cgcgtcctga gtgaaatcga cgccgaagta gcttccccag
     3241 gtcattccca tcgcattgat gaaatagttc caggtcagga aactcaccat caccgccaga
     3301 caagcacgtc cctgcgcttg cttagcaagg ccaatgggta aaccgacagc aaaaatcagc
     3361 ggcatattac ggaataccgt ccaaccgccc tcttcaataa tgtgtacgat ttgcgcgaat
     3421 aaactgttcg gatcggtcag tgattccccg acaaacatcg ggttttgcag caagatggca
     3481 agacccacca caatcccggc gaagggaaac agcagcactg gcgtgaacat cgcgccgcca
     3541 aagcgttgaa tttgactgag catttccaca tcctcttttc tcaattctgt aagtaggaag
     3601 cctgattggt tatgcggcag acctggagcg aggataggaa gatgcgatcg gcagaacatg
     3661 acgacccaat gggattcgtg atcgctttca tgcttttcgt tgggcggtag agatctacgt
     3721 tttcgcgaaa aagtagacat ctgtgagcac aggtacacag catcgaacac atctttaaaa
     3781 aaaagatttt tttcaatcga ttaagcagaa cttgtgggcg cattacccgt gcttgcaggc
     3841 aaaaaagaga gctagagatg atctacaaaa gcattgcgga gcggttaaga attcgactta
     3901 attccgcgga tttcacgcta aacggccttc ttcccggtga aaaaaagctg gcggaagagt
     3961 ttgcggtatc gcggatgact atccgtaaag ccattgacct gctggtagcg tgggggctgg
     4021 tggtccgccg ccacggcagc ggcacttacc tggtgcgcaa agatgtgctg catcaaaccg
     4081 ccagcctgac cggactggtg gaggtgttaa aacggcaggg aaaaacggtc accagccagg
     4141 tgctgatttt tgaaatcatg cctgcgcctc cggccattgc cagccagtta cggattcaaa
     4201 tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt ggaagggaaa ccgctaatgc
     4261 tggaagacag ctatatgccg gtaaaactgt tccgtaacct ttcactgcaa catctggaag
     4321 ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt gattggcggt aattatgaaa
     4381 gcctgatgcc ggtgctcgcc gatagactgc tggcgcggca aatgaaggta gcggaacaca
     4441 cgccactgct gcggatcacc tcgttgtcat atagcgagag cggggagttt ttgaattatt
     4501 cagtgatgtt cagaaatgcc agcgaatact aggtggagta ccatttacgg cgactccacc
     4561 cggaaaagag ttaaccgata ctccagaaga gcaccgccag taattggggg gtgataatgc
     4621 gcaggaacat cactaacgga tagacagtgg cgtaagagag cgccgccgca ccactggttg
     4681 gatgaagatt attagcaaac gccagcgccg gaggatcggt catggaacct gccagcatcc
     4741 cgcacatggt caggtaattc attttggcta acatccgcgc cagaatgcca acagtaatca
     4801 gcggaacggc ggtgatcaga gcaccataac caatccagct tagcccttcg ccattgacca
     4861 gggtattcac aaaatcccca ccagatttca gaccaacgac cgagaggaac agcacgatcc
     4921 ccaactcccg cagcgcgagg ttggcacttg gcggcataaa ccagtacagc ttgccgatac
     4981 tgccgatacg cccgaggatc aacgccataa tcagcggacc gcccgccagc cccagtttca
     5041 acgcggccgg gaatcctggc acaaagacgg gaatagaacc taacaatacg ccaagcccga
     5101 tgccaataaa cactggcagc atctgaacct gttgcagttt ttgctgcgca ttccccagca
     5161 cattggcaac ggcatcaatt gcggacggac gccccaccag gttgaggata tcgccgaact
     5221 gcaggctgat atcgccactg gcgaccagtt cgaccccggc acggttcagg cgcgagataa
     5281 caacgtcata gcgttcttta aagtgcaggt cgcgaatacg ttttccgagc acgttttcat
     5341 tggtgaccac cacacgctcg acgcgcaaat cagtgccttt cgtagacagc gaggtatcga
     5401 cctcctgacc aatcaccagt tgcgcattat gtaaatccgc tggctgcccc accagatgca
     5461 gcaaatcgcc cagttggata atggtatctg gcgaaggtac ttttagggtt tcttctcgtt
     5521 tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg tacatcttta atggctaaat
     5581 catgcaggtt agggttctca acgcgaatat tgatagtctt gatcagcgcg ccgccattgg
     5641 tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt gacgcggaaa ataacccgca
     5701 acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg atacgccatc gcgtaactca
     5761 tccccatctg atcgaccatt tccattggtg tacccaggtc gcgcaagatc tgctgccctg
     5821 cccccagcgc tggcgtattg gttaccgcac cggagaaaat ccccagcact accggcagtg
     5881 gaatatcaaa cagtttatgc aggatggcgg taaccagacc accgatgatg acgatcagaa
     5941 cagcaaacag gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag cccggcccta
     6001 cctgaatccc gatggtataa acgaacagga tcaggccaaa ttcctgaata acatgcagca
     6061 tatcgctact taatgtcatc cccgcctgag aaacaaaatg gccgacgatg atcccaccaa
     6121 acagcacgcc gccaatacct aatcctatgc cgcgaaattt gacgttgccg ataaacaaac
     6181 cgacgactgc caccaaagcc agaatactga ccgttaatgc tatatcactc atcgtccatt
     6241 tctcttgaga taacatttta attgctacgg attatggctg aatcggtgaa gcagatttgc
     6301 cttatagcgc acattatcac gggaaagaac gtgccgaaat atcttaaaca gtcgaaattt
     6361 aacgcattgt tgaagaagaa agacaaaaaa agccccacca gaatggcggg gcaaagagaa
     6421 tagctagtta gctatttaac gcgggacgtt cgctgatagc gatacgctgc gctgcgatgg
     6481 gttcaggctc attacgaatt aaatcaatat gcagtaaacc gtttacgaag gttgcgccag
     6541 agacttccat attttcagcc agcgtaaaac tcaggctaaa tggctgattc ataagccctt
     6601 gatgcagcca ttttttctct tcttttggct gctccggcgt gccttttacg ctcaggcgcg
     6661 taccttccag ttgaatctct aaatcttcct gacggaaacc tgccagcgca agggtaatgc
     6721 ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg gaagctctgg ctttcaccgg
     6781 cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc agtggggata
     6841 aatcgaagtt acgcatagtc atttctcctt ctaagaagcg agtaagtacc tgcaaatccg
     6901 aagattcgca tatgctccct gacggcgagc atggagatgt caggccgcgc caggcggcct
     6961 tagggaatta gttgatttcg atacggcgcg gttttttcgc ttccggaatc acgcgttcga
     7021 gatcgatata cagcaaacca tttaccaggt tagccccacg aacatgaatg ttctcagcta
     7081 actggaattt gcgttcaaag ttgcgttcag cgatgccctg gtacagatag gtgcgctctt
     7141 tttgttcgtc ggcgtgagca cctttcacca ccagcagatt atcctgggcg gtaatttcca
     7201 gttcgctctc agcaaaacca gccacagcga tagcaatgcg gtaatggttt tcgtctacca
     7261 gttcaacgtt atacggaggg tagccgccat tactctggct ctggttgttt tctaagtggt
     7321 taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg ggataaatca aagttacgca
     7381 taatcaatag ctcctgaaat cagcgagaat gtaagacctt ccacaatgga caggtcaggt
     7441 agccagaaca cccatcaggc gcgtcctcat cgactacgat gtaaaatggg tctggaaatg
     7501 aacttttcaa gccttatcag cggacttttt tttgcagttt atggtctatt gcatagactg
     7561 agggggcagc acgaaacgtt aaagtgcgat agccgccaca gtgcgacgta atgatgatgt
     7621 tattttcagc aagggatcgc tataactcat caggccaaca cccacgatca acagatgaat
     7681 ttattatgat aagaaatgtg ttgttagcgt tcatgatatg cagcggaatg acattactcg
     7741 gaggatgctc cagcgtgatg tcccataccg gggggaaaga aggaacgtat cccggcaccc
     7801 gcgccagcgc caccatgatt ggcgacgatg aaaccaactg gggcactaag tcgctggcga
     7861 ttctcgatat gccgtttacc gctgtgatgg acacgctttt actgccgtgg gatgtgttcc
     7921 gcaaagacag ctcggtgcgc tcgcgcgttg aaaaaagcga agccaacgcg caagccacca
     7981 atgcggttat tccaccggcc agaatgcctg ataactgatt tagcgatcgg tttcggtgat
     8041 ccacagctgg ccaccttcca tccacgccag ccattgacca tccggcgaga agaccacggc
     8101 gtccgcagaa ggtggattgg cgtggttttc ggttaaatac tcaacctcgc cgctttgcgc
     8161 atgggcacaa acaattcgat tatccagcac aaagcccaac cattctcctg acggatgcca
     8221 gttaaatgca gactgaatat ccgttttgtt atgggttaac ttgcgcggtt cgccgccctg
     8281 tggcgagata agccacagtt gcacaatgcc gttatcatca cgcattaaaa acgcgatttg
     8341 cgtaccctgc ggattacagc gcacccagtg gcgcgggtag ggttgtttcc gttccactta
     8401 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
     8461 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
     8521 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
     8581 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
     8641 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
     8701 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
     8761 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
     8821 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
     8881 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
     8941 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
     9001 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
     9061 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtga
     9121 gcaggtgatt acggggagca aaggttatct gtttcatggc gcggtcctga tacaaaacgg
     9181 ttcccgccat catacttcac aaggacttca ctttcagaca atagtctttt gccatcgtga
     9241 tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat tattggcctc ggcccggcag
     9301 ggtcggcgtt ggcacgaaag ttagccggca aaatgcaggt gatcgcgctg gataaaaagc
     9361 accagtgcgg tactgaaggt ttcagcaaac cttgcagatt ttcagcgtca aaactgtcga
     9421 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9481 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgatagcct
     9541 gtgccgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9601 ggagcaacat attaccgccc gctatctggt cggtgccgat ggcgcaaact cgatggtgcg
     9661 gcgacatctc tacccggatc atcaaatccg taaatatgtc gctatccagc agtggttcgc
     9721 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
     9781 ctcatggagt atcagcaaag atggttattt tatctttggc ggtgcctatc caatgaaaga
     9841 cggtcagacg cgtttcacga cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg
     9901 caaagcggtg aaaagtgaga aatgcacggt gttgtttccc tcacgctggc aggattttgt
     9961 ctgtggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10021 gctggagggg attagctatg cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa
    10081 acagccggag aagagcaacg ccgcttactg gcgcgccaca cgcaaactgc gtttaaaact
    10141 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10201 gcgcagtggt gtggcgcata ttccacagtt gaaagattat ccaacgcgct tcacatcacc
    10261 caccagcagg atgtaagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10321 aggcgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10381 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10441 agaagagacc agcgaccagg taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat
    10501 caggcacata atcatcatcg gatcgttggt gtagttagcg cccataatgc aggtggagat
    10561 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10621 cagcagatcc gcgacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10681 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10741 gaaccaggtt aagaaaaacc acagtgcaga agccaccgca aattgcccaa gataaacgcc
    10801 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgcgcctc
    10861 tttcttcacc ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    10921 agctttgctg atacctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    10981 ccagataatg ccgataccac cagtgacaat gaacacccag tgccagctca acatctcctg
    11041 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11101 accaacggcg gaagcgcgtt catgttccgg aaaccagctg gtcaccatcc ggttattggt
    11161 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11221 tcccgtggca aagccctgga ataaagtcgc cactgaccaa ccaaatatcg caataaaata
    11281 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11341 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11401 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11461 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11521 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11581 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11641 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagcgg attacgccag
    11701 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac acctaagccc
    11761 ggtttcgtta acggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    11821 aagtcgagta actccgcgcc tttgttgtaa tgaatgccca tactttgtta ctgaagtacc
    11881 gcgttatagg aaacaaagtc gatatgcagg caagccgcca gtgcaatcgg tccgaacgga
    11941 cagtgcggcg caagggtcac atcataggct tctgccattc cagcaatttt gtagcattcg
    12001 gtaataccgc ctgcatggga gagatccggt tgcagaatcg aaataccgcc tgcctccagc
    12061 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12121 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12181 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12241 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12301 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12361 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taatgccgtc gataacatcc
    12421 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12481 atcagttgcc agactggcgc attcagcact ttgcctttga tatcccataa cgcctggtcg
    12541 atcccggcga tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12601 acttgccata agtcgttgat gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg
    12661 tgaactgccg cttccaccgt acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc
    12721 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    12781 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    12841 gcgcggtgcg ttctacggac tgcccggcgc gataaagatc gctgcctaag cccgccccaa
    12901 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    12961 ctgcgatgtc cggtggtaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13021 atgacggaaa tattttcatc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13081 ccgtcgcgca gccggggcaa acggtcatgc cgtagcccac cgcgcggcgg atcacttcac
    13141 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13201 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13261 cgggaatgct ctgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13321 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg
    13381 cgatcagcgg gagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13441 gccgtgtcgc ccgccaccgc cgccacgtcg caacccatcg cctgaaaggc ttgctggtag
    13501 cgcgcggtca gcgatgttcc ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg
    13561 cgcatactgg caacctctgc gccaatcaac aaaccagaga gaaattcgct gacctgttcg
    13621 cggagaagtg ttcccagcac atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg
    13681 ggcgcattaa ggccgcgctc aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc
    13741 ggcaaacctg cgccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    13801 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    13861 ggcataacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg
    13921 ccgcgcatca cattgtggtt atcgtcatga gagacgcata atccgggaat aatccagata
    13981 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aacgggcagg aacagataaa
    14041 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccatcac cactggcgta
    14101 ttttcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14161 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14221 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14281 cctttaacct tcgtgtcgag ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc
    14341 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14401 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14461 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14521 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14581 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14641 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14701 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    14761 acgccccgcg ataacgtttc atttcaatca gccgcttcgc catcagcgaa cggaacacct
    14821 cacggatgat gttgcgcgag gttgcaaact cttcacagag ctccgcctca gccggaagtg
    14881 gcgagccagg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat
    14941 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15001 cgatcgatgc ataacgcggc aactttgtag taccagcgtg atgacgttcg cgtttgccgt
    15061 gcgtgtaatg tagtacaaac ttatattgtc gtgctacaat ttagatcaaa aaaagaacaa
    15121 tgcataaaaa aatgacatgc gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca
    15181 ggagggaaag aagagtatgc tttcaacggc ttagctactc gtttaaaaga ttaatcatga
    15241 agttgaattt taagggattt tttaaggctg ccggtttatt cccactggcg ctgatgcttt
    15301 caggctgtat ctcgtatgct ctggtttccc ataccgcaaa gggtagttca ggaaagtatc
    15361 aatcgcagtc agacaccatc actgggctat cgcaggcaaa agatagtaat ggaacaaaag
    15421 gctatgtttt tgtaggggaa tcgctggatt accttatcac tgatggtgcc gatgacatcg
    15481 ttaagatgct caatgatcca gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa
    15541 gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac aatatcgctt tactactact
    15601 ggaataacga agaagaaaag gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac
    15661 actgtaccag gtcactggaa aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg
    15721 attactcaaa ggtgatggcg ttctaccatc catttaaagt gcgattttat gaatactatt
    15781 cacccagagg cattccggat ggtgtttccg cagcattact gccagtgact gttacgctgg
    15841 acatcattac tgcaccgctg caatttctgg ttgtatatgc agtaaaccaa taatcagtaa
    15901 gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt aattcagcac atacttctca
    15961 atagcaaacg ccacgccatc ttcaaggttg gattttgtga caaagttcgc catttctttc
    16021 accgaaggaa tagcgttatc catcgccaca ccgacgcctg catattcaat cattgcgatg
    16081 tcgttttcct gatcgccaat cgccatgatt tcttccggtt taatacctaa cacgtcggcc
    16141 agtgatttca cacccgtacc tttgttaacg cgtttatcga ggatttcgag gaagtacggc
    16201 gcacttttca gcacggtata tttctctttc acttcctgcg gaatacgcgc gatagcctgg
    16261 tcgaggatgg cgggttcatc aatcatcatc actttcagga actgggtatt ggggtccatt
    16321 ttctccgctt cgcagaacac cagcggaatt gtggcaacga aggattcatg caccgtgtag
    16381 tagctgatat cgcggttagc ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa
    16441 ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt catagctgag agcggtttgc
    16501 gccacggtgc taccatcagc ggccttctgt accagcgcgc cgttataagt aatgcagtag
    16561 tcgcccggct gttccatatg cagctctttc aggtagttgt gcacacctgc atacggacga
    16621 cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg cggcaatcgc atttttaacg
    16681 gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat ccatatcgat agcaatgagt
    16741 ttgatagcca tgagttccca aggtagatta gttcctgacc catgctaacg cgattccgct
    16801 caaaaatcag tacaacaccc gagggaaaag gggatgcaac gcgggttgca tgctccccat
    16861 ttgcttagcg gaagagtttc cctttcagca gttccatgcc tgcggaaagc agatcgttat
    16921 tggcttgtgc tgacacttca ccttgcggtg agagcgcatc aataatcttc ggcaattgct
    16981 ctgccagtaa actggaagcc gtactggtat ccacgccaag tttttgcccg agatcggaca
    17041 ccgcatttgt gccgagcgcc gattccagtt gctcgccgct aactgattga tttctctgtt
    17101 gattactcaa ccaagttgag agaatggccc ctaagccgcc actttgcagt ttttccagca
    17161 gcacctgaat gccaccctgc tcctcaaccc aacttaaaat cgcctgatat ttccccgcat
    17221 cgcctttcag aaaggcaccg acaacttcat caaaaagccc catgataatc acctgtaaag
    17281 cgttacgtgt tgacccaaaa agtatagatc tgcgggatga taattgcgga ttgcagaaat
    17341 aaaaagggcg gagatgatct ccgccctttt cttatagctt cttgccggat gcggcgtgaa
    17401 cgccttatcc ggcctacaaa atcatgcaaa atcaatatat tgcaggagtc tcgtaggact
    17461 aataagcgta gcgcatcagg cacgctcgca tggttaacgc cattaaatat caatattcgc
    17521 cgctttcagg gcgttctctt caataaacgc acgacgcggt tctacggcgt cgcccatcag
    17581 cgtggtgaac aactggtcag cggcaatcgc atccttaacg gtaacgcgca gcatacgacg
    17641 gctttccggg tccatggtgg tttcccacag ctgttccggg ttcatctcgc ccagaccttt
    17701 ataacgctgg atggagaggc cacgacggga ctctttcacc agccagtcca gcgcctgctc
    17761 gaagctggct accggctgac gacgctcgcc acgttcgata aacgcatctt cttccagcaa
    17821 gccacgcagt ttctcaccca gcgtgcagat acgacgatat tcgccaccgg tgataaactc
    17881 gtgatccagc ggatagtcag tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa
    17941 caggttttgt tcggcattgg tgtggacatc aaacttccac tggctgccgt gctgttcttt
    18001 gtcgttcagt tcgctgacca gcgcgttcac ccagcgggta acggtctgct catcagaaag
    18061 gtcagcttcc gtcaacgtcg gctgatagat aagctctttc agcattgctt tcggataacg
    18121 acgctccatg cggttgatca ttttctgcgt cgcgttgtac tcagatacca gtttctctaa
    18181 cgcttcgcca gccaatgccg gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc
    18241 gatagagatc tggtactgat ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc
    18301 tttcttcact ttgtacagcg gcggctgagc gatgtagacg tgaccgcgtt caacgatttc
    18361 cggcatctga cgatagaaga aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc
    18421 cgcatcggtc atgatgatga tgctgtgata acgcagtttg tccgggttgt actcgtcacg
    18481 accgatacca cagccaagcg cggtgataag cgtcgccact tcctgagaag agagcatctt
    18541 atcgaagcgc gctttctcga cgttaaggat tttacccttc agcggcagaa tcgcctggtt
    18601 cttgcggtta cgcccctgct tcgcagagcc gcccgcggag tccccttcca ccaggtacag
    18661 ttcggaaagc gccggatcgc gttcctggca gtctgccagt ttgcccggca ggccagctaa
    18721 gtcgagcgca cctttacggc gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg
    18781 ggcagcatcg ataattttgc caaccacgat tttcgcatca gttgggtttt ccagcaggta
    18841 ttccgccagc agttcgttca tctgctgttc aaccgccgat ttcacctcag aagaaaccag
    18901 tttgtctttg gtctgggagg agaatttcgg gtccggcact tttacggaaa cgaccgcaat
    18961 caggccttca cgcgcatcgt caccggtggc gctgactttg gcttttttgc tgtagccttc
    19021 tttgtccatg taggcattca gggtacgggt catcgccgca cggaagcctg ccaggtgagt
    19081 accgccgtca cgctgcggaa tgttgttggt aaagcagtag atgttttcct ggaagccatc
    19141 gttccactgc agcgccactt caacgccaat accgtctttt tcggtggaga agtagaagat
    19201 atttgggtgg atcggcgttt tgttcttgtt cagatattca acgaacgctt tgatgccgcc
    19261 ttcatagtgg aagtggtctt ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc
    19321 ggagttgagg aacgacaact cacgcagacg tttcgccaga atttcatatt cgaactcggt
    19381 cacattggtg aaggtttcga ggctgggcca gaaacgcacc atggtgccgg ttttttcagt
    19441 ctcgccggta accgccagcg gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat
    19501 tttaccctcg cgctgaataa ccagctccag tttttgcgac agggcgttta ctaccgaaac
    19561 accaacgccg tgcagaccgc cggacacttt ataggagtta tcgtcaaatt taccgcctgc
    19621 gtgcagaacg gtcatgatca cttccgccgc cgatacgccc tcttccgggt gaataccggt
    19681 cggaatgccg cgcccgtcat cctgtacaga gacagagtta tcggcgtgaa tggtgacgat
    19741 aatttcttta cagtgacccg cgagcgcttc gtcgatagcg ttatctacca cctcgaatac
    19801 catgtggtgc agaccggtgc cgtcatccgt gtcgccgata tacatacccg ggcgcttacg
    19861 caccgcatcc agccctttca ggactttgat actggaggag tcataagaat tcgacatcaa
    19921 cgtttctcgc tcatttatac ttgggttaat ccgttatttt acccttttcc acggtaaaca
    19981 tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc actgatcgcg ctgacaaaga
    20041 cctgtgattg cgtcgctttt aagcggctgg caagcaaccc gcgacgctca tcatcaagct
    20101 cagaggcaaa atcatctatc aggtagagac accgccgccc gctttcacga gtgaggaact
    20161 ctccttgcgc cagacgtaag gcgcacatca acagcttaag ctgcccacgc gataaggtat
    20221 cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg
    20281 tgtaggttag ctggcgatcg cgttcaaaat tacgttccag cacctcagcg tactctgtct
    20341 ctttctccca gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga aattgcttac
    20401 aggtatcatc catatcggcc gcgataccgg cgctatactc cgcgcgccag gtgctgattt
    20461 gctccgccag cgggatcagt tctttatccc acgggcgtag ctgttcgtaa cgagtcacct
    20521 ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag attgctccag gcggtgaaaa
    20581 atccaggttc gttgtgaaag catccccagt cgaggaatgc tcttctgtat ttggggccgc
    20641 cgttgagtaa agtaaaccct tctggcgtta tcaactgcat cggcatcagg tgcgccagtt
    20701 ccgcgacttt atgcccgtcc gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt
    20761 tggttaagcc aatcgccgtc tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg
    20821 cctcctgctc atggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc
    20881 cgagcgtata gatggcttcc agcacgctgg ttttgccact gccgttggca cctaccagaa
    20941 agttaaagcc gggagataaa gcgagatccg cggtttcaat attgcggaaa tcgcggatca
    21001 acaagcgggt gagggacatt acagtctcat tggcatgaca acataggcag ccgactgtga
    21061 tgcggcatct tcaatctgca cgctggaaac cgaatcggtc agcatcatgc ggacgttttc
    21121 gcatttcagc gcgttcagaa catccagcac atagctgacg ttgaagccga tttccatctc
    21181 cgcaccgcta taggtaacgt cgaggatctc ttccgcttct tcctgttccg ggttgttggc
    21241 agtaattttc agctggtttt cgctgacata caggcgcaca ccgcggaatt tctcgttaga
    21301 gagaatcgcc gcgcgagcaa acgcctgctt gagcagatcg cagcctgctt ccagatgttt
    21361 gtccgggttc ttcggcagaa cgcgacggta atccgggaag cgaccatcca ccagtttcga
    21421 ggtgaaaata aagtcgccaa cgtgggcacg aatgttattg ctgccaatct gcacacgcag
    21481 cagattgtcg ccgccgtcga gcatacgcat cagttcaatc acgcctttac gcggtacgat
    21541 caccgaatgg cttggcaaag attgaccaat tggcattgaa cagaccgcca gacggtggcc
    21601 gtcggttgcc acggtgcgca gttcttcacc ttcggtttca aacagcatac cgtttaagta
    21661 atagcgaaca tcctgatgcg ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt
    21721 cgcctgctgc agggtaaatt cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc
    21781 cgctggcagg gtagacagcg aaaaacggct acgcccggag cgcaccagca tccgttcacc
    21841 ttccagctgc acggcaattt ccgcgccttc aggcagacca cggcagatat caaagaattt
    21901 gcgcgccgga acggtcgtcg ctcctggctc gtgtggctga accagcgcaa cgcgtgccac
    21961 catttccatc tcgagatcag taccggtcag cgacaacgta ccgtcagcaa cctgtaacag
    22021 cagattaccg agaatcggta gcgtaggacg accacctaac ggaccgctca cctgttgtag
    22081 cggttttaat aaatgctcac gttctacggt aaatttcata ggtttacgat gacaatgttc
    22141 tgattaaatt tgaaaaatct tctttgatat cgtggctctc ttcacgcaac tgctcgatct
    22201 tacggcaggc atgaagcacc gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg
    22261 gcagactgtg gttagtcagc tctttcgcta gcgccatcgc catctggcgc ggacgcgcca
    22321 ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt gatcttgtag tactccgcca
    22381 ccgtcttctg aatattgtcg atggtgacca gtttttcctg caatgccagc aagtcgcgca
    22441 gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt aaagttggcg ttagcaataa
    22501 cgcggttcag cgccccttcc agctcacgta cgttagatcg tagacgcttg gcgataaaga
    22561 acgccacttc acccggcaaa cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca
    22621 cacgggtttc cagctctggc ggttcgatcg ccacagtcag tccccaaccg aagcgggatt
    22681 tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg atccgaggtg agaatgatct
    22741 gttgattacc ttccagcagg gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt
    22801 tattagcaaa aaactgaata tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt
    22861 taaactcttc gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg
    22921 agtgcatata aaccactttg gcattcggct tgcgcgccat aatgccgtta cccaccgcat
    22981 gcagcaggtg agttttaccc agacccgtgc cgccataaag gaacaacggg ttataggcac
    23041 cgccagggtt atccgccacc tggcgagccg ccgcgcgcgc cagttggtta gatttacctt
    23101 caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga acgataggtc ggttctgccg
    23161 gggccgggac gttatcccaa cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt
    23221 gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca
    23281 ccggtttggt gccgacttca aaacgcagct gtggggcatc cgctccgcag aaactggtta
    23341 gcagtccatt gatattatta aggtacttgt cccgtaccca atcgaggaca aaacggtttg
    23401 gcgcgtacag ggacagcgtg ttatcgctca gttccgcctg caatgggcgt atccacatac
    23461 tgaattctgt ggctggtaac tcatcctgca atcgggcaag acactgctgc caaagcgaaa
    23521 gtgacacggc ggactccact cgaacaaaag tcgataatga ctaaggctga aacattcatg
    23581 attgttgacg tacgtcgaaa agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg
    23641 ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg cggatcatag cctaaactgc
    23701 gcaagagatc ttctgtttct cacagatttt ccctatttat ccacaggact ttccagaact
    23761 cgctaagtgt aaacgatcct gccgcgaggc gggcacgatt tacgccgcat attggaaaat
    23821 ttaatgacca tagacaaaaa ttggcttaat cgatctaata aagatccagg acgatccttg
    23881 cgctttaccc atcagcccgt ataatcctcc acccggcgcg ccatgctggt ttccactggt
    23941 gtgaggtcgt acattttccc tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag
    24001 aattgactcc ggtgtgtaca attattacaa tccggcctct ttaatcaccc atggcttcgg
    24061 tgtccatcgt ttcatttttc ggcggatatc caataaagcc attgaattta ttcaagttta
    24121 ggtagaaatc gccatgaaac gcacttttca accgtctgta ctgaagcgca accgttctca
    24181 cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc
    24241 taaaggccgc gctcgtctga ccgtttctaa gtaataaagc taacccctga gtggttaagc
    24301 tcgcatttcc cagggagtta cgcttgttaa ctcccagtca attcacattc gtcttccagc
    24361 agccacaacg ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc
    24421 atccccgtat cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc
    24481 ggattaaacg tctgacgcgt gaaagcttcc gtctgcgcca acatgaactc ccggctatgg
    24541 atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga taaccgtgct ctctcggaag
    24601 cgttggaaaa attatggcgc cgccactgtc gcctggctcg cgggtcctga tagccctcat
    24661 tcgggtctat caacgcctga ttagtccgct actcgggccg cattgtcgtt tcactccaac
    24721 ctgttcaagc tacggaattg aggcattgcg caggtttgga gtgataaaag gcagttggtt
    24781 gacggtgaaa cgcgtattaa aatgccaccc tttacaccct ggtggtgacg atcccgtccc
    24841 gcccggacca tttgatacca gagaacacta acgatggatt cgcaacgcaa tcttttagtc
    24901 atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct gggagcagga taaaaacccg
    24961 caacctcagg cccaacagac cacgcagaca acgaccaccg cagcgggtag cgccgccgac
    25021 cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat
    25081 ctgaccatca acacccgtgg tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa
    25141 gagctgaact ctacccagcc gttccagctg ttggaaactt caccgcagtt tatttatcag
    25201 gcacagagcg gtctgaccgg tcgtgatggc ccggataacc cggctaacgg cccgcgtccg
    25261 ctgtataacg ttgaaaaaga cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg
    25321 ccgatgacgt ataccgacgc ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt
    25381 ggtgattacg ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa accgctggaa
    25441 atctcctcgt ttggtcagtt gaagcaatcc atcactctgc caccgcatct cgataccgga
    25501 agcagcaact tcgcactgca caccttccgt ggcgcggcgt actccacgcc tgacgagaag
    25561 tatgagaaat acaagttcga taccattgcc gataacgaaa acctgaacat ctcttcgaaa
    25621 ggtggttggg tggcgatgct gcaacagtat ttcgcgacgg cgtggatccc gcataacgac
    25681 ggtaccaaca acttctatac cgctaatctg ggtaacggca tcgtcgctat cggctataaa
    25741 tctcagccgg tactggttca gcctggtcag actggcgcga tgaacagcac cctgtgggtt
    25801 ggcccggaaa tccaggacaa aatggcagct gttgctccgc acctggatct gaccgttgat
    25861 tacggttggt tgtggttcat ctctcagccg ctgttcaaac tgctgaaatg gatccatagc
    25921 tttgtgggta actggggctt ctccattatc atcatcacct ttatcgttcg tggcatcatg
    25981 tacccgctga ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct gcagccgaag
    26041 attcaggcaa tgcgtgagcg tctgggcgat gacaaacagc gtatcagtca ggaaatgatg
    26101 gcgctgtaca aagctgagaa ggttaacccg ctgggcggct gcttcccgct gctgatccag
    26161 atgccaatct tcctggcgtt gtactacatg ctgatgggtt ccgttgaact gcgtcaggca
    26221 ccgtttgcac tgtggatcca cgacctgtcg gcacaggacc cgtactacat cctgccgatc
    26281 ctgatgggcg taacgatgtt cttcattcag aagatgtcgc cgaccactgt gaccgacccg
    26341 atgcagcaga agatcatgac ctttatgccg gtcatcttca ccgtgttctt cctgtggttc
    26401 ccgtcaggtc tggtactgta ctatatcgtc agcaacctgg taaccattat tcagcagcag
    26461 ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga
    26521 ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc gccttttttc ttttcgtagg
    26581 gcggataagc accgcgcatc cgccacacaa agcaacagga acatcatgag cgataatgac
    26641 actatcgtag cccaggccac acctccggga cgtggcggcg ttggcatcct gcgcatctcc
    26701 ggcttcaaag cccgtgaagt tgccgaaacc gtgctgggta aactgcctaa gccgcgctac
    26761 gccgattatc ttccgtttaa agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta
    26821 tggttccctg gcccgaactc gttcaccggc gaagatgtgc tggaactgca aggtcatggc
    26881 ggtccggtga tcctcgacct gctgttaaaa cgcattctga ccattcccgg cctgcggatt
    26941 gctcgccctg gtgagttttc cgaacgcgcg tttcttaacg ataaacttga cttagcccag
    27001 gccgaggcga ttgccgatct tatcgacgcc agttcggaac aggcggcccg ttcggcactt
    27061 aactcgctgc aaggcgcatt ctccgcacgg gttaatcatc tggtagaagc cctcacccac
    27121 ttgcgcattt acgtcgaagc ggcaattgat ttccctgatg aagagatcga tttcctctcc
    27181 gacggcaaaa ttgaagccca gcttaatgac gtgatggccg atctcgatgc agtgcgtgct
    27241 gaagcacgtc agggtagttt gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct
    27301 aatgccggta aatcgagcct gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc
    27361 gatatcgccg gaaccacgcg tgacgtgctg cgtgagcata tccacattga cggaatgccg
    27421 ctgcatatca tcgacactgc cgggctacgt gaggccagtg acgaagtgga acgtattggt
    27481 atcgagcgcg cgtggcagga aattgaacag gccgaccgcg tgctgtttat ggtcgatggc
    27541 accacgacag acgccgttga tccggcagag atctggccgg aatttatcgc ccgtctgccc
    27601 gcgaaactgc cgatcaccgt ggtacgcaat aaagccgata tcaccggcga aacgctggga
    27661 atgagtgaag tgaacggtca cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg
    27721 gacgtgctgc gtaaccatct caaacagagc atgggctttg acaccaatat ggaaggcggc
    27781 ttcctggcgc gccgtcgcca cttacaggcg ctggaacagg cagcggagca tttgcagcaa
    27841 ggtaaagcgc aactgttggg agcctgggca ggtgaactgc tggcggaaga gttgcgcctg
    27901 gcacagcaga acttaagcga aatcaccggg gaatttactt cagacgacct gctggggcgg
    27961 attttctcca gcttctgtat tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc
    28021 agatgcggct tcgcttcatt gttagcactc ctgttatgcc tcaacccttt tttaaacatt
    28081 aaaattctta cgtaatttat aatctttaaa aaaagcattt aatactgctc cccgaacgat
    28141 tgtgattcga ttcacattta aacaatttca gaatagacaa aaattctgag tgtaataatg
    28201 tagcggcgtg tctttcgagg ataagtgcat tatgaatatc ttacatgtat gtgtgacctc
    28261 aaaatggttc aatatcgaca ataagattgt cgatcaccgc ccttaattta cccttctgta
    28321 gccatcacca gagccaaacc gattagattc aatgtgatcg attagtttgc tatatcttaa
    28381 ttttgccttt tgcaaaggcc atctctcgtt tatttactta ttttagtaaa tgatggtgct
    28441 tgtatatata catggtgaat taatggatat tgcagatgta atattcacag ggatcactgt
    28501 aattaaaata aatgaaggat tatgtaatgg aaaactttaa acatctccct gaaccgttcc
    28561 gcattcgtgt tattgagcca gtaaaacgta ctac
//
LOCUS       DADMFL010000047        28462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.047, whole genome shotgun sequence.
ACCESSION   DADMFL010000047 DADMFL010000000
VERSION     DADMFL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.047"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1114
                     /gene="pdeC"
                     /locus_tag="JNZ27_002017"
     CDS             <1..1114
                     /gene="pdeC"
                     /locus_tag="JNZ27_002017"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY8664354.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQG"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNZ27_002018"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNZ27_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY8664355.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JNZ27_002019"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JNZ27_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY8664356.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /locus_tag="JNZ27_002020"
     CDS             2778..4127
                     /locus_tag="JNZ27_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8664357.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JNZ27_002021"
     CDS             4278..5927
                     /locus_tag="JNZ27_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY8664358.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JNZ27_002022"
     CDS             complement(6081..7373)
                     /locus_tag="JNZ27_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8664359.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DQDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNZ27_002023"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNZ27_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY8664360.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JNZ27_002024"
     CDS             complement(9197..9511)
                     /locus_tag="JNZ27_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY8664361.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNZ27_002025"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNZ27_002025"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY8664362.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JNZ27_002026"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JNZ27_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY8664363.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JNZ27_002027"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JNZ27_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY8664364.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JNZ27_002028"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JNZ27_002028"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY8664365.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JNZ27_002029"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JNZ27_002029"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY8664366.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKARALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JNZ27_002030"
     CDS             15750..17468
                     /locus_tag="JNZ27_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8664367.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JNZ27_002031"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JNZ27_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY8664368.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JNZ27_002032"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JNZ27_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY8664369.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JNZ27_002033"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JNZ27_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY8664370.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JNZ27_002034"
     CDS             complement(20170..20859)
                     /locus_tag="JNZ27_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8664371.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNZ27_002035"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNZ27_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNZ27_002036"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNZ27_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY8664372.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNZ27_002037"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNZ27_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNZ27_002038"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNZ27_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY8664373.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFISAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDAQVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="JNZ27_002039"
     CDS             complement(27862..28137)
                     /locus_tag="JNZ27_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664374.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28462)
                     /locus_tag="JNZ27_002040"
     CDS             complement(28346..>28462)
                     /locus_tag="JNZ27_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664375.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6483 a         7209 c         7645 g         7125 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atagtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gtttctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttg atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agctcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaactcaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgggctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc tgcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagata aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcggcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gc
//
LOCUS       DADMFL010000048        28091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.048, whole genome shotgun sequence.
ACCESSION   DADMFL010000048 DADMFL010000000
VERSION     DADMFL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.048"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(163..513)
                     /gene="ubiK"
                     /locus_tag="JNZ27_002041"
     CDS             complement(163..513)
                     /gene="ubiK"
                     /locus_tag="JNZ27_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY8664376.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
     regulatory      575..723
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            826..1479
                     /gene="ribB"
                     /locus_tag="JNZ27_002042"
     CDS             826..1479
                     /gene="ribB"
                     /locus_tag="JNZ27_002042"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY8664377.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1740..1910
                     /locus_tag="JNZ27_002043"
     CDS             1740..1910
                     /locus_tag="JNZ27_002043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664378.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1968..2741)
                     /gene="zupT"
                     /locus_tag="JNZ27_002044"
     CDS             complement(1968..2741)
                     /gene="zupT"
                     /locus_tag="JNZ27_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY8664379.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2857..3672
                     /gene="ygiD"
                     /locus_tag="JNZ27_002045"
     CDS             2857..3672
                     /gene="ygiD"
                     /locus_tag="JNZ27_002045"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY8664380.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3710..4870)
                     /locus_tag="JNZ27_002046"
     CDS             complement(3710..4870)
                     /locus_tag="JNZ27_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8664381.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4876..5547)
                     /locus_tag="JNZ27_002047"
     CDS             complement(4876..5547)
                     /locus_tag="JNZ27_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY8664382.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5695..7176)
                     /gene="tolC"
                     /locus_tag="JNZ27_002048"
     CDS             complement(5695..7176)
                     /gene="tolC"
                     /locus_tag="JNZ27_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY8664383.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7381..8010
                     /gene="nudF"
                     /locus_tag="JNZ27_002049"
     CDS             7381..8010
                     /gene="nudF"
                     /locus_tag="JNZ27_002049"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY8664384.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8011..8433
                     /locus_tag="JNZ27_002050"
     CDS             8011..8433
                     /locus_tag="JNZ27_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY8664385.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8458..9285
                     /gene="cpdA"
                     /locus_tag="JNZ27_002051"
     CDS             8458..9285
                     /gene="cpdA"
                     /locus_tag="JNZ27_002051"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY8664386.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9285..9866
                     /gene="yqiA"
                     /locus_tag="JNZ27_002052"
     CDS             9285..9866
                     /gene="yqiA"
                     /locus_tag="JNZ27_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY8664387.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9895..11787
                     /gene="parE"
                     /locus_tag="JNZ27_002053"
     CDS             9895..11787
                     /gene="parE"
                     /locus_tag="JNZ27_002053"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY8664388.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11835..12149)
                     /locus_tag="JNZ27_002054"
     CDS             complement(11835..12149)
                     /locus_tag="JNZ27_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8664389.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12180..12761)
                     /gene="mdaB"
                     /locus_tag="JNZ27_002055"
     CDS             complement(12180..12761)
                     /gene="mdaB"
                     /locus_tag="JNZ27_002055"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY8664390.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13080..13412
                     /locus_tag="JNZ27_002056"
     CDS             13080..13412
                     /locus_tag="JNZ27_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY8664391.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13458..14807)
                     /gene="qseC"
                     /locus_tag="JNZ27_002057"
     CDS             complement(13458..14807)
                     /gene="qseC"
                     /locus_tag="JNZ27_002057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY8664392.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14804..15463)
                     /gene="qseB"
                     /locus_tag="JNZ27_002058"
     CDS             complement(14804..15463)
                     /gene="qseB"
                     /locus_tag="JNZ27_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY8664393.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15615..16007
                     /gene="ygiW"
                     /locus_tag="JNZ27_002059"
     CDS             15615..16007
                     /gene="ygiW"
                     /locus_tag="JNZ27_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY8664394.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            16060..16542
                     /locus_tag="JNZ27_002060"
     CDS             16060..16542
                     /locus_tag="JNZ27_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8664395.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17088..19346
                     /gene="parC"
                     /locus_tag="JNZ27_002061"
     CDS             17088..19346
                     /gene="parC"
                     /locus_tag="JNZ27_002061"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY8664396.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19580..20317
                     /gene="plsC"
                     /locus_tag="JNZ27_002062"
                     /gene_synonym="parF"
     CDS             19580..20317
                     /gene="plsC"
                     /locus_tag="JNZ27_002062"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY8664397.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20392..21804
                     /gene="ftsP"
                     /locus_tag="JNZ27_002063"
     CDS             20392..21804
                     /gene="ftsP"
                     /locus_tag="JNZ27_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY8664398.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21915..24134
                     /locus_tag="JNZ27_002064"
     CDS             21915..24134
                     /locus_tag="JNZ27_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY8664399.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIILSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24177..24434)
                     /gene="yqhH"
                     /locus_tag="JNZ27_002065"
     CDS             complement(24177..24434)
                     /gene="yqhH"
                     /locus_tag="JNZ27_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY8664400.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24485..25411)
                     /locus_tag="JNZ27_002066"
     CDS             complement(24485..25411)
                     /locus_tag="JNZ27_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY8664401.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25611..26438)
                     /gene="dkgA"
                     /locus_tag="JNZ27_002067"
     CDS             complement(25611..26438)
                     /gene="dkgA"
                     /locus_tag="JNZ27_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY8664402.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26543..27706)
                     /gene="yqhD"
                     /locus_tag="JNZ27_002068"
     CDS             complement(26543..27706)
                     /gene="yqhD"
                     /locus_tag="JNZ27_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY8664403.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            27900..>28091
                     /locus_tag="JNZ27_002069"
     CDS             27900..>28091
                     /locus_tag="JNZ27_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8664404.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6590 a         6954 c         7453 g         7094 t
ORIGIN      
        1 tatcgattaa gtaaaactta aagaatacat aacaattata ttttcaattg caatcattca
       61 tttttgtgat gatactctga atgggattaa gcgataatgt aatagaaaac actgtaaatt
      121 agaggattaa ttcataattt acagtgcatt aaagacaaat gtttacagcg ttggggggag
      181 agtctctgga tctggctgct ttttgatctc agtactgcga ttttccagtt cgctgattct
      241 ttgttcaagc agcgccagtt tctcacgagt ccgtaacagg acttgcgttt ggacgtcgaa
      301 ctcttcacgg cttacaaggt cgaggcgcgt cagctgagct tgtagggttt ggcggatttt
      361 tttctccaca tcttccccga actccctgat tcctttaggc attgattcgt gaacctggcg
      421 agcgatttgc tcaatttttt tcgggtcaat cattgtagtt tccctgtact gataggtgtt
      481 gagtaccatt gtagtgcgat aagggtaagt cataaaccag aattatgtga agctatgcgt
      541 tgctgccgct aatcattaga gttatagtga atccgcttat tctcagggcg gggcgaaatt
      601 ccccaccggc ggtaaatcaa ctcagttgaa agcccgcgag cgctttgggt gcgaactcaa
      661 aggacagcag atccggtgta attccggggc cgacggttag agtccggatg ggagagagta
      721 acgattctgt cgggcatgga cccgctcacg ttattttggc tatatgccgc cactcctaag
      781 actgccctga ttctggtaac cataatttta gtgaggtttt ttaccatgaa tcagacgcta
      841 ctttcctctt ttggtacgcc tttcgaacgt gttgaaaatg cactggctgc gctgcgtgaa
      901 ggacgcggtg taatggtgct tgatgatgaa gaccgtgaaa acgaaggcga tatgatcttc
      961 ccggcagaaa ccatgaccgt tgagcagatg gcgctgacca ttcgccacgg tagcggtatt
     1021 gtttgcctgt gcatcactga agatcgccgt aaacaacttg atctgccaat gatggtagaa
     1081 aataacacca gcgcctatgg caccggtttt accgtgacca ttgaagcggc tgaaggtgtg
     1141 actaccggtg tttctgccgc tgatcgtatt acgaccgttc gcgcagcgat tgccgatggc
     1201 gcaaaacctt ctgatctgaa tcgcccgggc cacgttttcc cacttcgcgc gcaggcaggt
     1261 ggtgtactga cgcgtggcgg tcatactgaa gcgactattg atttgatgac gctggcaggc
     1321 tttaaaccgg ctggtgtgct gtgtgagctg actaatgacg atggcacgat ggcgcgtgca
     1381 ccagagtgca ttgagtttgc caataaacac aatatggcgc tcgtgactat tgaagacctg
     1441 gtggcatacc gtcaggcaca tgagcgtaaa gccagctgaa aaccgctgct taattactgc
     1501 cttaatcaag aaaccgaagt tgtagctggc ttcggttttt attttgccct gctatgcaag
     1561 atgttaattc cgcttctctt tctatgagaa aatttcatta atatcaggca ttctttttca
     1621 ttatgatcaa aatcatataa ttgttccgtt attatgcgac catatgaata tatccgaaaa
     1681 aggatatgtg aaatttatgc gctaaatgtt tagtattgtt aaatattaag ggtaaaagga
     1741 tgtttatcgc ctggtactgg attgtattga ttgctctggt tgtggtgggt tatttcctgc
     1801 atttgaaacg ttattgtcgg gcgtttcgcc aggacagaga cgcactgctt gaagcgcgga
     1861 acaaatactt aaacagtacg agagaagaga cagccgaaaa ggtagaataa ggctttcgtg
     1921 aagctggcag aagttgtcca tgtgctggac aacttctgat gttgttttta accaattccc
     1981 gccgtttgta gcagcactaa actgaatccc atcactgaca ttccacatag tacgccatag
     2041 ctggggttat tattagggtc aatctctttg gcgagcggca ttaattcatc aaccgagagc
     2101 gccaccataa ttcctgcaac cgccgccatg attgccgcca tgaccaccgg ggaaatcatg
     2161 ctaccgagga ttaaccacgc cagcacacca ccaagaattt ctgctagtcc agaaatcccc
     2221 gcccacagaa tcgcggtgcg tttagacccc gttgccgcat aaaccgggcc tgccactgcc
     2281 agaccttcag ggatattgtg caacgcgacg gccagtgcga tgccaaatcc cagctccagg
     2341 ttgctgctcg ccgtgacaaa ggtggcaatc ccttccggga agttatgcag actgatgccg
     2401 agagtgagca gaatggctgt gcgcttgatc gattttggca acagctgcac cgatttttgc
     2461 attaaatcct gcggatgcgc atgcggcaac atgcggtcca ggccaaagta gccaagcaga
     2521 ccgaagataa acattccata acccaacacc ggcgacattc cttcagctgc tagtgcggca
     2581 ggaagcattt ccattaatga gatgagcaac atgatccccg ccgcaaaacc tagcgaaaac
     2641 gccagtaagc ggttcgaggg tttttgtccg agaacgccga gaaacgcgcc aataaacgtg
     2701 gctgcccccg ccagtatggt cagaatgaga ggtactgaca tcgacaactc cttataaatc
     2761 ttcaccttca ggcagatcgt caaaataact gatgatcatc atcattgtta tccgggttct
     2821 tactccgccg aacaagcgta ttgtgaggat atcaaaatga cacctttagt taaggatatc
     2881 atcatgtctt caacacgtat gccagcattg tttttaggtc acggtagtcc gatgaacgtg
     2941 ctggaagata atttgtatac ccgcagctgg cagaagttgg ggatgacatt gccacgcccg
     3001 caagcgattg tggtggtttc ggctcactgg tttacccgtg gaacaggagt gaccgcgatg
     3061 gagacgccgc ccacgattca tgactttggt ggcttcccgc aggcgctgta cgatacgcat
     3121 tatcctgctc cgggttcgcc tgcgctggca cagcgtctgg ttgagctgtt agcgccgatc
     3181 ccggtgacgc tggataaaga agcctggggc tttgaccacg gctcgtgggg cgtgctgatt
     3241 aagatgtatc ctgacgccga tatccctatg gtgcagttga gtatcgacag tagcaaacct
     3301 gccgcctggc atttcgaaat ggggcgcaaa ctcgcggcgt tgcgtgatga aggaataatg
     3361 ttggtcgcca gcggtaacgt ggtgcataac ctgcgcacag tgaagtggca cggtgatagt
     3421 tcaccgtatc cgtgggcgac gtcgtttaat gagtatgtga aagcgaatct gacgtggcaa
     3481 gggccggtag agcagcatcc tctggtgaat tatctcgacc atgaaggtgg cgcgttatcg
     3541 aacccaacgc cagagcacta tctgccgttg ttgtatgtgt taggtgtgtg ggatgggcag
     3601 gagccaatta ccattccggt cgatggtata gaaatgggca gtttaagtat gctgtcggtg
     3661 cagataggct aacaattacc ggatggcgag tgccatccgg tatcgtggct tattcaacaa
     3721 aaatatgtgg ataaaaccga gacatatcct gggtgatcaa cgcacggtct tcacgaatgc
     3781 caattccggc aggttgatcg ttcaccagcc agctaccaat cagcatatag ctgtcgccga
     3841 atttcggtaa cgggtggaat tgctgaacaa tcatcccttc ttcgccatac ggaccttccg
     3901 ctgcttcaat ggttttgccg ttctcaatga tcgacacgtt tgcgccttca cgggagaaga
     3961 tcggtttaac cacatatttt tccatttgcg gatgatcatc ttccgcaaaa taagcgggca
     4021 gcaggttcgg gtgattcggg aacatctccc acagtagcgg tagaagtgcc ttgttggaga
     4081 taatactctt ccacgccggt tccagccagc gtacgccagc atcttccagc ttggtggaga
     4141 acatttcgcg cagcataaat tcccacggat aaagcttgaa caggttagaa atcacctgat
     4201 cctgtaaatc cgtgaactgg cctttttcgc ctaaacctat ctcgtcgatg tagaggaact
     4261 cggtggcgat ttcagcttcg gttgcgcagt cctgcaaata ctgaatggtt ccgcgatctt
     4321 ccaccgtgtc gcgacagcag gtgagatgca gcaactggaa gccacactgt tcacgcagct
     4381 caacgaagcg atcgatcagt ttttcttgca gactgttaaa ctggtcgctg ccctccggca
     4441 agttaccggc gttaagctga tcttccagcc agatccactg aaagaacgcc gcctcgtata
     4501 gtgacgttgg cgtatcggcg ttattttcca gaagtttagg ttcgccagtg ccatcccacg
     4561 ccagatcaag acgcgaataa agcgatggct ggtgagtcag ccatgattga cgcacaaaac
     4621 tccaggtgtg ttttggtatg cggaatttgg tcatcagctc atcgctggcg atcacttttt
     4681 ccaccacttt caggcacatc tggtgcagtt cggcggtgac ttcttccagc ttttcaacct
     4741 gggcgagggt caacttgtag taagcatctt cacaccagta cggctcgccg tacatggtgt
     4801 gaaaattgaa accgtattcg tgggctttct cacgccagtc cgggcgctcg gtaatactga
     4861 ctctttccat cggtatcagc cacccattga acgagaagag gtgccggttg cgctacgctg
     4921 catagtgctt tgtttggcaa cagattcacc aaaaccgcca cgggtaacgg tagtggtggt
     4981 cgccggtttt ggtgccattg ccgtcttcgg tacggtcatg gtgcggcctg gctgggctgc
     5041 gccatagttt ttacccgtcg cgtcggtata tttaccgtaa gccggactgg ccgggttttt
     5101 cgaggagaac agcggctgct gtgcaaatcc cgcgccgccg cccatcagac gccccatcat
     5161 gtaaccggcc atcagcggca tccagaaact cccgctggat tgctgagcct gcgcctggtt
     5221 ttctggtgcc atgccagcct gggctggtgc ttgctgacac tgaccttcac caaattcagc
     5281 aacacagtct tcacgggtgg cgtatttcgg cgcagtacgt tcggcttctt tcagcgcatt
     5341 gttgtacgcg gtggtacatt cggcgctttt gcctgggttt gcagctgaac agtcgtcagc
     5401 attttgatag agagacactg tttcatcact cttttcacag ccagccagca taaaaacagt
     5461 ggcaaccgcg agagcgactg gtgtcagatg gcgtgcgctc cagtttttgc ggaacgatgc
     5521 gtggcgtatg gattttgtcc gtttcatttt tgtcttccgg gaccagtggt aaatacccat
     5581 cagaatagag gatggctggt cgaaattgaa gcgagaaggg ggaagaatgc ggcagataac
     5641 ccgtatcttt acgttgcctt acgttcagac ggggccgaag ccccgtcgtc gtcatcagtt
     5701 acggaaaggg ttatgaccgt tactggtggt agtgcgtgcg gatgtttgct gaacgacggg
     5761 tgccgggcta tcaggcgcat aaccatcagc aatagcattc tgttccggcg tttgcggggc
     5821 aacgttttcc ggattagtgg aaaccggttt gctcagcgca ttgttcagtg ccagcaaatc
     5881 ctgctcgttc aacgtaccca gggctgactt aatattcagc tgattaatca ggtagttata
     5941 acgcgcattc gccagctctt gcttggcgtt gtacagcgtg gtggtcgcat ccaacacatc
     6001 aacaatggta cgcgtaccga ccgagtagcc cgcttccatc gcgtctaatg agctttgcgc
     6061 ggaaactacg gcttgtttgt aggcgttaat actactgata gaagcattaa tgttgttgaa
     6121 ggaggaacgt acggtttgca cgacgctacg atgtgcgctt tccagttgct cactggcacc
     6181 aacaaagttg tactgtgcct gtttcacctg cgagttaacc attccgccct gataaatcgg
     6241 cagcgagaag ctcaggccaa ctttgttctg gcccatattg ctatcgtcat actgggtacc
     6301 agcggcacca cgggttttcg aaccgctata agaggtgtca gaaatcccgg tagaagccgt
     6361 taaatccaga gtcggtaagt gaccatcctg cgcctggcga atttgctcgc gcgccaggtc
     6421 ctggctcaag cgtgcctgta acagcgacag gttgcgtttt tcggcttctt tcagcagcgc
     6481 gttaaccggc tgtggtttgt cggttttaaa gttttcgaca ttcagcgccg ccagttccgg
     6541 atagtagtta ccggtgatct ggcgcagctg ctctaccgcg ttatcaaggt tattacgtgc
     6601 ggtcacttcg ttcgccagca cggtatcgta ctgcgcgcgg gcgttctgca cgtcggtgat
     6661 cgctaccagg cccacgttaa aacgttgggt ggtttgatct aattgacggt agatcgcttc
     6721 tttttgtgcc tgtgtatagg aaagaacgtc aatagcattc aacacgttga aataagcggt
     6781 cgcggtgttg aggatcaagg tttgctgatc ggtctgatat gtgacgtcct gaatccctgc
     6841 tgctttttcc tgcagcgtta acgcacgcca tttcgacata tcaaaaatgg attgagttaa
     6901 ctgcagggac gcactggtcg cgttcgagtt gatgccgttc gcgtcgcggt agccgttgct
     6961 ataggtgtaa tctgcaccta aacctagctg tggcagtaat ggactgcgcg cttcattaat
     7021 tttttcaaag gcagcatcac gatcggcggc agacttacgc aattccgggt tactaaggcg
     7081 tgcttgctga taaacttgca tcaggttctc ggcctggctc aacgaactga acccagaaag
     7141 gctcaggccg ataagaatgg ggagcaattt cttcatttgc attccttgtg gtgaagcagt
     7201 atttagcgcg atcaaactgt aaaattattg cggattctag cagaagccgc taccgcaaaa
     7261 ggttggcgtt acgtgccata gggcgttaat gtgcctcaat ttaacatatg actgctcaaa
     7321 cggcagtcaa acgtcgctga aattcacatt taattcacta ttagtgccag gacattaaca
     7381 atgcttaagc cagacaacct gcccgttaca tttggcaaaa acgatgtaga aattattgca
     7441 cgagaaacac tttatcgcgg ctttttttca ttagatcttt atagatttcg tcatcgtcta
     7501 ttcaacgggc aaatgagtca tgaggtacgg cgggaaattt ttgagcgcgg tcacgccgca
     7561 gtcttgctac cctttgaccc agtgcgtgat gaagttgtgc tgattgagca gattcggatt
     7621 gccgcgtacg acaccagcga aaccccctgg ctactggaga tggttgccgg gatgattgaa
     7681 gagggtgaaa gtgtggaaga tgttgcccgt cgcgaagcga ttgaagaggc gggactgata
     7741 gtcaaacgga ccaaaccggt gttaagtttc ctggcaagcc cggggggcac cagtgagcgt
     7801 tcgtcaatta tggtgggcga agtggacgcc acgaccgcaa gcggtattca tggtctggct
     7861 gatgaaaacg aagatattcg cgttcatgtg gtaagccggg aacaggcata ccagtgggta
     7921 gaagagggga aaatcgacaa cgcagcgtcg gtcatcgctt tgcaatggct gcagctgcat
     7981 catcaagcgt taaaaaatga gtgggcataa atgaagcgtt acacacctga ctttcctgaa
     8041 atgatgcgcc tgtgcgagat gaacttttca caattgcgcc gtttgttacc gcgcaatgac
     8101 gcacccggcg aaactgtaag ctatcaggtg gcaaacgcac aatatcggct gacgattgtg
     8161 gaatcgaccc gatacactac cctggtgaca atagaacaga ctgcgcccgc gatcagttac
     8221 tggagccttc cgtcaatgac ggtgcgtctg tatcatgacg cgatggtggc tgaagtgtgt
     8281 tcaagccagc agatttttcg cttcaaagcg cggtatgatt atcctaataa aaagttgcat
     8341 caacgcgacg aaaagcatca aattaaccag tttttagcgg actggttgcg atactgttta
     8401 gcacatggag cgatggcgat tccggtttac tagcgtcgtg aaacctaagg acaccatttg
     8461 gaaagcctgt taacccttcc tctggctggt gaggccagag gaaggatttt acaaattacc
     8521 gatactcacc tgtttgcaca aaagcacgaa gccctgttag gggtaaacac ctgggaaagc
     8581 taccaggcgg tgctggaggc gattcgtcca caccagcacg aattcgacct gattgtcgcg
     8641 acaggtgatt tagcgcagga tcaatcctct gcggcctatc agcatttcgc tgaaggcatc
     8701 gcaagttttc gtgcgccctg cgtctggctg ccgggcaacc acgatttcca gcccgcgatg
     8761 tacagcgcgt tacaggatgc gggtatctcc ccggcgaagc gggtgtttat tggtgagcaa
     8821 tggcaaatcc tgttgctgga tagccaggtg tttggcgtgc cgcacggtga gctgagcgag
     8881 tttcagcttg agtggctgga acgtaaactg gccgatgcgc cagaacgcca tacgttgctg
     8941 ctgctgcatc atcatccgct acctgcgggt tgtagttggc tcgatcaaca cagtctgcgt
     9001 aacgcgggcg aactggatac cgtgctggcg aagtttccgc acgtcaaata cttgctgtgc
     9061 ggtcatattc atcaggagct ggatctcgac tggaacggtc gccgcctgct ggcaacgccg
     9121 tcgacctgtg tgcagtttaa gccgcactgt tccaacttta cgctggatac tatcgcgccc
     9181 ggctggcgta ctctcgagtt acatgctgat ggcacgctga ccaccgaggt gcatcgcctg
     9241 gcggacacac gtttccaacc tgataccgct tcagaaggct actgatgtct acgcttcttt
     9301 atttacacgg tttcaacagc tcgccgcgct ctgcaaaagc gagcttgtta aaaaactggc
     9361 tggcggaaca tcaccctgac gttgagatga tcattccgca gttgccgccg tatccttccg
     9421 acgcggcaga actgctggaa tccattgtcc tggaacatgg cggcgattcg ctgggtattg
     9481 tcggttcgtc actgggggga tattacgcca cctggttgtc gcaatgtttt atgctgcccg
     9541 cagtggtggt aaacccggcg gtacgcccgt ttgaattgct gacggactat ctcggtcaga
     9601 acgagaaccc ctacaccggg cagcaatatg tgctagagtc acgccatatt tacgatctca
     9661 aagtcatgca gattgacccg ctggaagcgc cggatttgat ctggctgctg caacagacgg
     9721 gagatgaagt gctggattac cgccaggcgg tggcgtacta cgcttcctgc cgccagactg
     9781 tcatagaagg cggcaaccac gcattcacgg gcttcgaaga ttatttcaac ccgatcgtcg
     9841 attttcttgg tctgcaccat ctctgacgat caactcgaat tgctaactta aaccatgacg
     9901 caaacttata acgctgatgc cattgaggta ctcaccgggc ttgagccggt tcgccgccgt
     9961 ccggggatgt ataccgatac cactcgccct aaccatttgg ggcaagaagt tattgataac
    10021 agtgtcgatg aagcactggc gggtcacgca aaacgcgtgg atgtaatctt acatgctgac
    10081 cagtcgttag aagtgattga cgatgggcgc gggatgccgg tggatattca cccggaagaa
    10141 ggagtaccgg cggttgaact gattctttgc cgtctgcatg caggcggtaa attctcgaac
    10201 aaaaattacc agttctctgg cggcctgcat ggcgtgggga tttcggtagt taacgccttg
    10261 tcgaagcgcg tagaagttaa cgtgcgccgc gatggtcagg tttataacat cgcctttgaa
    10321 aatggcgaaa aggtgcagga tttacaggtt gtcggcactt gcggtaaacg caataccggt
    10381 accagcgtgc acttctggcc ggatgaaacc ttctttgaca gcccgcgttt ttctgtttca
    10441 cgcctgacgc atgtgctgaa ggccaaagca gtactgtgcc ccggcgttga gattaccttt
    10501 aaagatgaga tcaacaacac cgaacagcgc tggtgctatc aggacggtct gaatgattac
    10561 ctggcggaag cggtaaacgg tttaccgacg ctgccagaaa aaccgtttat cggtaatttc
    10621 gctggcgata ctgaagctgt ggactgggcg ctactgtggc tgccggaagg cggtgaactg
    10681 ctgaccgaaa gctacgtcaa ccttatccca acgatgcagg gcggtaccca tgttaatggt
    10741 ctgcgtcagg gcctgttgga cgcgatgcgt gagttctgtg aataccgcaa cattctgccg
    10801 cgcggcgtga agctgtcggc ggaagatatc tgggatcgct gcgcctatgt tctgtcggtt
    10861 aaaatgcagg atccgcagtt tgccgggcag accaaagagc gtctctcttc gcgtcagtgt
    10921 gcggcattcg tttcgggcgt ggtgaaagat gccttcatcc tgtggctgaa ccagaacgtt
    10981 caggcggcgg agttgctggc agagatggcg atttccagcg cccagcgtcg tatgcgtgcg
    11041 gctaaaaaag tggtgcgtaa aaagctgacc agcggcccgg cgctgcctgg caaactggct
    11101 gattgtaccg cgcaggacct taaccgtacc gaactgttcc tcgtggaagg tgactccgca
    11161 ggcggatctg ccaagcaggc gcgcgatcgt gaatatcagg cgatcatgcc gctgaaaggt
    11221 aagatcctta acacctggga agtttcttcc gacgaagtgc tggcctcgca ggaagtgcat
    11281 gatatttcgg tagcgatcgg tatcgatcct gacagcgacg atctgagcca gcttcgttat
    11341 ggcaaaatct gtatcctcgc ggatgcggac tctgatggtc tgcacattgc cacgctgctc
    11401 tgcgctttgt tcgtaaaaca cttccgcgcg ttggtgaaac acggtcacgt ttacgtcgca
    11461 ctgccaccgc tctaccgtat tgatctcggg aaagaggttt attacgcgct gacggaagaa
    11521 gagaaagagg gcgtacttga gcaattaaaa cgcaagaaag gcaagccgaa cgtccagcgt
    11581 tttaaaggtc tgggggaaat gaacccgatg caattgcgtg aaaccacgct tgatccgaac
    11641 actcgccgtc tggtgcagtt gactatcgat gatgaagacg atcagcgtac tgacgcgatg
    11701 atggatatgc tgctggcgaa gaaacgctcg gaagatcgcc gcaactggtt gcaagagaaa
    11761 ggcgacatgg cggagattga ggtttaaagg aaagaatatt gcccggtcag gatggccggg
    11821 caaacaaggc aggattaaat ccctggctgc agaatacgga tattcatctc cagcacgtcg
    11881 ccttttacgg ctttgctgta cgccttcatg tgcggggttt gcagatgcgc ttcaaggtgc
    11941 gcgatgcttt cccactgctc aatcatcacg atagaatccg gtgccataga ctggaaactc
    12001 acgccagcag cgcaatccac cattggcgca tagccgtggc aaccttcttc tttcagtacg
    12061 gttggaacga ttttagcaaa ctgatccaat accgcctgac ggtgatgttg accaggacga
    12121 gtacggattt ctgcgattac ggtaagcatg gttaactcct tctaaagcct gagctctagt
    12181 taaccaaaaa tttccacaag atgcttgcga tattcttcag tatagcgggg aacatcaggc
    12241 atttttatca cgtcattagc gataaatgtc ggcagcggtt ccataccgag gaattggttt
    12301 gctttatgga acggcagata cacaccgtca acgccaacgc cgtggaagaa ctgatctttt
    12361 tcggtgaagg cttccattgg tgcgttccag gttagagaaa gcatatattt tttgccctgt
    12421 accaggccgc cggaaccgta ttttttcgac ggatctttgc gggtacgacc atcgctggca
    12481 tacagagtcc cgtgaccttc ggtgaataca tcatcaatat attttttcac tgtccacggc
    12541 gcgcccatcc accagcctgg catctgccag atcaccacat cagcccagag aaagttttgt
    12601 acttccgctt tgacattgta gtcgctgtcg gcgcgaacga tgcggacatc atgcccgagg
    12661 tcgcgcagtg tgccatccgc gacttcggtc agggtgtcgt tcagttgacc attggagtgg
    12721 gcgaattttt tcgcgccgtt gataatcagg atgttgctca ttttttaacc tcaaggtgag
    12781 acgtttgtcg actatagtac gtcacagagc ggtacggtga aattagcaaa atgtgcaaag
    12841 tcttttgcga atttagcaaa agtctgatct agctctgact caccgatgca ggataaatat
    12901 tttctgaaaa tgacaatact gatagccatt cctcataatt cttactcgta ccggaataat
    12961 ttacacgttt ggttcggttt ttttggggat tgaaaatatg gctttgaata agtaaaagat
    13021 gaaaatatca acgtgtcatg ttgataatta taaatggatt tttatgagcg tactgatata
    13081 tgttaaagca gaaaattaaa acaatatttg aagcattgct atatataatg cttacttatt
    13141 ggttaataga ttcttttttt gctttcaata agtatgactg gatgcttgag tcgggaggca
    13201 atatttgcag tataccttcc gtttcaggcg aagatcgtat attgcaagca atgattgcag
    13261 cattttttct tcttacgcca cttattattc ttatcttaag aaaactcttt atgcgggaaa
    13321 tgttcgagtt ttggctatat gtattctcgc tcgttatttg tctggtttgt ggttggtggc
    13381 ttttttgggg acggtttata ttttgctatt agtaaaatct atgagagtcg ttttaacggc
    13441 tctcatagac agagaagtta ccagcttacc ttcgcctcaa atccaccttg ttccgcattc
    13501 ccaaactcaa cattcatgtc atgcagcttg gcgattcgct ggacaatcga tagcccaagt
    13561 ccactgccgg tagcggtttg tccgggtggg cgatagaagc gttcgccaat tcgcgccagt
    13621 gcctctggtg tcacaccggg gccgttatcc ctcacgatga aattatcagc attcagcgtg
    13681 acgtctatca cgctgccctg tggactgtag cgcacggcgt tatccagcaa atttcgcacc
    13741 aacaaactta gcaatagcgg ttgtccggtg cgtttgatgc cgtgggcatt gagtgtcagt
    13801 cgcacgtcaa ttttcgcctg ctgcgccgtg tggtaaatat ccatcaccga cgattgtagg
    13861 agatcttcaa gcgggatctc cgcgacgtcc tgaaggttat ccagtgagtc cagccgcgat
    13921 agcgtgagca gttgatcaac cagacgagta gcgcgatcga tcccggaatg taattggagc
    13981 agtgcttttt tccgcgcctg cggatcatcg tcagagagct gcgcaacttc ggtttgcact
    14041 ttcagcgctg ttaacgggct acgtagttcg tgagctgcat cggaggtaaa gcgtcgttct
    14101 cgaaccatca tcgcatgtgt gcgggcgaac agttgattta gcgactcaac cagtggccgc
    14161 acttcgctgg gtacgccagt cgcgtttagt ggtttttccg agtcagggtc acgcatacgt
    14221 agtgccagcg ccagtttgtt cagcggcgcg agttcacgac ccagtagtac catcatgatg
    14281 attaacataa tcggcagtgc gaccagccac gggatcaatt gcccggcaac aatcgccagc
    14341 gccatgtctt cacggtattc ccattcctgg ccaacaacga tgcgatattt gccatcaggt
    14401 gaggtcatcc agacaaaacg ccaaggatcg tcttcaccga ccagttgccc gtcagcaaaa
    14461 ccttcccgtt gatagctata gggaatatct tctccgttat cgccatcatt aaggaccatt
    14521 ctgccgtcgt gggtaaagat ggcaaaggtc agcgcatcgt catcaacatg accgtgtttt
    14581 aatttatttg gcgtctgtgc catgcgatcc gccgcgttga tttcgttgag atcgggcgta
    14641 cttaaccgtt tggcaaacag catcagttgg gtgtcgaaca attcatcgac gttatccgtt
    14701 gtttgtttcc aggcgacaaa gctggaaagc agccaggtca ccgaggccag aattaaaaag
    14761 attagcgtca gcctgacgcg cagactaaga cgttgggtaa atttcatttc tcacctaatg
    14821 tgtagccaat accatgcacg gtacgaataa aatcactacc gagtttgcgt cgcagatgat
    14881 gcacatgcac ttcaacggca ttactggtga cctcttcgtc ccaggtatac agtttctctt
    14941 caatcagttt gcgcggcagt acccgaccag cgttacgcat cagtaattcc agcagggcaa
    15001 attcttttgg tttcagtgtt aagggttcgc cagccagcgt ggcgatacgt ttgccggggt
    15061 cgagcatgac gttaccgtgg cgcagctcgt tgctggcctg gccgttggtt cggcgcatca
    15121 gagcctccag cctggcggcg acttctatca acgcaaaagg tttacacaga taatcgtcag
    15181 ctcccagacg cagcccttct acacgttccg ctaacgcatc acgcgcggtc aggttcagta
    15241 ccggctcacg ctgacctttc tctcgccatt cgcgcaaaat atcgcgacca tccatgcctg
    15301 gtaaggttaa atccaggatc accgcatcat aaggtgcgct ataaagcgcc tcttttccct
    15361 gacgaccttg tgtaaaccag tcgacactaa aacccatttt actaaggcca gttttgatgc
    15421 cgtcaccaat cagcatgtca tcttctatca gtaaaattcg cattttttca tccctgcgat
    15481 aaccgtattt gctgccagta aaccgtgttg taacgcgctc tgtacagcat taaattttat
    15541 ttttttccct taagaagttg ttaaggacta tcttgttaaa tgctcggaaa cagacattaa
    15601 agggagtaat aaacatgaaa aaattcgcag cagtaatcgc agtaatggcc ctgtgcagtg
    15661 caccggtgat ggcagcagag cagggcggtt tttctggccc atcggcaacg caaagtcagg
    15721 ccggaggatt tcaggggccg aacggcagcg taacgactgt agaaagcgta aaatccctgc
    15781 gtgacgacac ctgggtaacc ctgcgcggca atatcgttga acgcatctct gacgatctct
    15841 acgtgtttaa agatgccagc ggtactatca atgttgatat cgatcacaaa cgctggaacg
    15901 gtgtgacggt gacgccgaaa gatacggttg agattcaggg tgaagtcgat aaagactgga
    15961 attctgttga aattgacgtc aaacagatcc gcaaagtaaa tccgtaattg ttaccgctcc
    16021 cgggacgcgt tcccgggaat aatttcgcag ggaggcaaaa tgacaaacct gacactggat
    16081 gtaaacatta tcgatttccc atcaatccct gtggcgatgt tgccgcaccg ctgtagccct
    16141 gagttgctca actacagtgt ggcgaaattt atcatgtggc gtaaagagac ggggctttct
    16201 cctgttaacc aaagccagac ttttggcgtc gcctgggacg accctgccac caccgcaccg
    16261 gaagcgtttc gctttgatat ctgcggcagc gttagcgaac cgattcccga taatcgttat
    16321 ggtgtgagca atggtgaact taccggtgga cgttatgccg tggcccgcca cgttggcgag
    16381 ctggacgata tttcacacac gatatggggc attattcgcc actggctgcc tgcaagcggc
    16441 gagaaaatgc gtaaagcacc gattctgttt cactacacca atcttgccga aggggtgaca
    16501 gagcagcgac tggaaacgga tgtttatgtg ccgttggcgt gatggggaat ctttaccgga
    16561 gtgtgagcca aatcactcgc gataagtttg agcgaacaat gtcacaggcg tattgttgtt
    16621 gggcttataa tttctccggt aaggaatacc atgtatacgc gaaatttatt atccggtgcg
    16681 ccgtaagccg tctcgggggt atccatgagc aagccgcact tatggcagga ggcgcggggc
    16741 actggtggag acacagctaa actgggctga accgtatact tacagtcgac tactttgatg
    16801 ctttaggctt ccctggactc gcataacctc aacttctcga atcgcccgga gcggaccccc
    16861 atgccgagtg gtgtggcatg ggcgcaacct tgtgctgccc tctatgccga tcaagtctac
    16921 agcaaagagt tgtatatcaa ggcacattga tgcctttatc gccgtcggag cacgtccggc
    16981 ggcgctattc acgctgatct cctgtgactc gacgcggcag ataatgtagt atctccggca
    17041 atattgcccc tttgaaggct ggcgaataag ttgaggaatc agaattaatg agcgatatgg
    17101 cagagcgcct tgcgctacat gaatttacgg aaaacgccta cttaaactac tccatgtacg
    17161 tgatcatgga ccgtgcgttg ccgtttattg gtgatggtct gaaacctgtt cagcgccgca
    17221 ttgtgtatgc gatgtctgaa ctgggcctga atgccagagc caaatttaaa aaatcggccc
    17281 gtactgtcgg tgacgtactg ggtaaatacc atccgcacgg cgatagcgcc tgttatgaag
    17341 cgatggtcct gatggcgcag ccgttctctt accgttatcc gctggttgat ggtcagggga
    17401 actggggcgc gccggacgat ccgaaatcgt tcgcggcaat gcgttacacc gaatcccggt
    17461 tgtcgaaata ttccgagctg ctattgagcg agctggggca ggggacggct gactgggtgc
    17521 caaacttcga cggcactttg caggagccga aaatgctacc tgcccgtctg ccaaacattt
    17581 tgcttaacgg caccaccggt attgccgtcg gcatggcgac cgatattcca ccgcataacc
    17641 tgcgtgaagt agctcaggcg gcaatcgcat taatcgacca gccgaaaacc acgctcgatc
    17701 agctgctgga tatcgtgcag gggccggatt atccgactga agcggaaatt atcacttcgc
    17761 gcgccgagat tcgtaaaatc tacgagaacg gacgtggttc agtgcgtatg cgcgcggtgt
    17821 ggaagaaaga agatggcgcg gtggttatca gcgcattacc gcatcaggtt tcaggtgcgc
    17881 gcgtactgga gcaaattgct gcgcaaatgc gcaacaaaaa gctgccgatg gttgacgatc
    17941 tgcgcgatga atctgaccac gagaacccga cccgtctggt gattgtgccg cgttccaacc
    18001 gcgtggatat ggatcaggtg atgaaccacc tcttcgctac caccgatctg gaaaagagct
    18061 atcgcattaa cctcaatatg atcggtctgg atggtcgtcc ggcggtgaaa aacctgctgg
    18121 aaatcctctc cgaatggctg gtgttccgtc gcgataccgt gcgccgccga ctgaactatc
    18181 gtctggagaa agtcctcaag cgcctgcata tcctggaagg tttgctggtg gcgtttctca
    18241 acatcgacga agtgattgag atcattcgta acgaagatga accgaaaccg gcgctgatgt
    18301 cgcggtttgg ccttacggaa acccaggcgg aagcgatcct cgaactgaaa ctgcgtcatc
    18361 ttgccaaact ggaagagatg aagattcgcg gtgagcagag tgagctggaa aaagagcgcg
    18421 accagttgca gggcattttg gcttccgagc gtaaaatgaa taacctgctg aagaaagaac
    18481 tgcaggcaga cgcgcaagcc tacggtgacg atcgtcgttc gccgttgcag gaacgcgaag
    18541 aagcgaaagc tatgagcgag cacgacatgc tgccgtctga acctgtcacc attgtgctgt
    18601 cgcagatggg ctgggtacgc agcgctaaag gccatgatat cgacgcgccg ggcctgaatt
    18661 ataaagcggg tgatagcttc aaagcggcgg tgaaaggtaa gagtaaccaa ccggtagtgt
    18721 ttgttgattc caccggtcgt agctatgcca tcgacccgat tacgctgccg tcggcgcgtg
    18781 gtcagggcga accactcacc ggcaaattaa cgttgccgcc tggggcgacc gttgaccata
    18841 tgctgatgga aagcgacgat cagaaactgc tgatggcttc cgatgcgggt tacggtttcg
    18901 tctgcacctt taacgatctg gtggcgcgta accgtgcggg taaggctttg atcaccttac
    18961 cggaaaatgc ccatgttatg ccgccggtgg tgattgaaga tgcttccgat atgctgctgg
    19021 caatcactca ggcaggccgt atgttgatgt tcccggtaag cgatctgccg cagctgtcga
    19081 agggcaaagg caacaagatt atcaacattc catcggcaga agccgcacgt ggcgaggatg
    19141 gtctggcgca actgtacgtt ctgccgccgc aaagcacgct gaccattcat gttgggaaac
    19201 gcaaaattaa actgcgtccg gaagagctac agaaagtcac tggcgaacgt ggacgccgcg
    19261 gtacgttgat gcgcggtttg cagcgtatcg atcgtgttga gatcgactct cctcgccgtg
    19321 ccagcagcgg tgatagcgaa gagtaatatg ccggggaggg cgctgtttcc tcccgctagc
    19381 aaggaacgcc ggatgaaacg ttaagtcgtc atccggcagc gtattcaacc cccggaaacg
    19441 ataaaaattc ccttgaaacc gttgtttatt catgcgttgc gattaacaat acgcttttcc
    19501 agagagcggc ttttaacaat gccctaacct gatttcaggt gacgtacaat gccagtctct
    19561 cagaccttca gagggtgtta tgctatatat ctttcgtctt attattaccg tgatttacag
    19621 catcttagtc tgtgtattcg gctccattta ctgccttttc agcccgcgta acccgaaaca
    19681 tgtggccacc tttgggcata tgtttggccg tcttgcgcca ctgttcggcc tgaaagttga
    19741 gtgccgcaaa cctgcagatg ctgaaagtta cggcaatgct atctatatcg ctaaccacca
    19801 gaacaactat gacatggtga cagcatcgaa cattgtgcaa ccgccgacgg tgacggtagg
    19861 taaaaagagc ttgctgtgga tccccttctt cgggcagttg tactggttaa ccggcaactt
    19921 attgatcgac agaaacaatc gcactaaagc tcacggcacc attgcggaag tcgtgaatca
    19981 tttcaaaaaa cgccgtattt ccatctggat gttcccggaa ggaacccgca gccgtggtcg
    20041 cggcctgcta ccgttcaaga ctggagcatt tcacgcggca attgcggcgg gcgtcccgat
    20101 tattcccgtg tgcgtctcta caacttcgaa taagattaat cttaatcgac tgcacaacgg
    20161 tctggtgatt gtcgaaatgc tgccgccaat tgacgtcagt cagtatggca aagatcaggt
    20221 tcgtgagctg gctgcccatt gtcgttcgat aatggaacaa aaaatcgccg agctcgataa
    20281 agaagtcgca gaacgcgaag ccgccggaaa agtttaagtc ggcaatctgt atttttgcgg
    20341 ggaacacttt cctgcacggt attactttag ccagttttac atggagcaaa tatgtcactc
    20401 agtcggcgtc agttcattca ggcatcgggg attgcacttt gtgcaggcgc tgttcccctg
    20461 aaggccagcg cagccgggca acagcaaccg ctacccgttc cgccgctact tgaatctcgc
    20521 cgtgggcaac cgctgtttat gactgtacaa cgtgcgcact ggtcatttac gccagggaca
    20581 cgcgcgtcgg tctggggaat caatggtcgt tacctggggc cgactatccg cgtctggaag
    20641 ggcgacgatg ttaagcttat ttacagcaac cgcctgacag aaaatgtctc aatgacggtg
    20701 gccgggctac aggtaccagg cccgctgatg ggcggtccgg cacggatgat gtcgccaaac
    20761 gctgactggg cacccgtact gcctattcgc cagaacgcag ctactctgtg gtatcacgcc
    20821 aatactccca accgcacggc tcagcaggtc tataacggcc ttgccggaat gtggctggtg
    20881 gaagatgaag tcagcaagtc gctgcctatc cccaaccatt atggtgtgga tgattttccg
    20941 gtcattatcc aggataaacg gctggataac tttggtacgc cagaatacaa cgaaccggga
    21001 agcggcggct ttgttggtga tacgctgctg gttaacggtg tacaaagccc gtacgttgaa
    21061 gtctcgcgtg gctgggtgcg cttgcgactg ctgaacgcgt cgaactctcg tcgctatcaa
    21121 ctacagatga gcgatggtcg cccgttacat gtgatttctg gcgatcaggg attcctgcct
    21181 gctcctgtat cggtgaagca actttcgctg gcaccgggcg agcgccgcga gattctggtg
    21241 gatatgagca acggcgatga agtgtcgatc acctgtggcg aagcggcgag cattgttgat
    21301 cgtattcgcg gcttctttga gccatccagt attctggttt ctaccttggt gctaacgctg
    21361 cgcccaaccg gccttctgcc gctggtcaca gacagtcttc cgatgcgctt gctgccaact
    21421 gaaatcatgg ctggttcgcc aattcgcagt cgcgatatca gtctgggtga tgacccgggt
    21481 attaatggac agctgtggga cgtcaaccgt attgatgtca ccgcgcagca aggaacgtgg
    21541 gaacgctgga cggtacgcgc ggacgagccg caagcgttcc atattgaagg cgtaatgttc
    21601 cagatccgta acgtgaatgg cgcgatgccg ttcccggaag acagaggctg gaaagatacc
    21661 gtttgggttg acggacaagt ggagctgctt gtttatttcg gtcagccttc ctgggcgcac
    21721 ttcccgttct acttcaacag tcagacgctg gaaatggcgg accgtggctc gattgggcaa
    21781 ctgttggtca atccggtacc gtaatcttca tactcgcccg aggagggcga gtatggaata
    21841 cctttcgatt tctctttcgt tccggcttat aatccccggc ctttgattat ttttttatca
    21901 tttctggaag caaaatgagc tctatctccc tgatccaacc ggatcgcgac ctgttctcct
    21961 ggccgcagta ctgggccgcc tgttttggac cggcaccgtt tttgccgatg tctcgtgaag
    22021 agatggatca acttggctgg gatagctgcg acatcatttt ggttactggc gacgcgtatg
    22081 tcgatcaccc aagcttcggg atggcgattt gcggtcgtat gctggaagcg cagggctttc
    22141 gcgtcgggat catcgcccag ccggactgga gcagcaaaga cgactttatg cgtctgggta
    22201 aaccgaatct gttttttggt gttactgctg gcaacatgga ttcgatgatc aaccgctata
    22261 ccgccgatcg ccgtttacgt catgatgatg cctacacgcc agataatgtc gcgggtaagc
    22321 gaccggatcg cgccacgctg gtttataccc agcgttgtaa agaggcgtgg aaagatgtgc
    22381 cggtgatcct cggcggtatt gaggctagcc tgcgccgtac cgcgcattat gattactggt
    22441 ccgataccgt gcgccgttcc gttctggtag attcgaaagc cgacatgctg atgtttggta
    22501 acggtgaacg tccgctggtg gaagtagcgc atcgtctggc gatgggcgag ccgattagtg
    22561 aaatccgcga tgtgcgtaat accgcaatta tcgtaaaaga ggcgctgcct ggctggagcg
    22621 gtgtggattc cacccgtctt gataccccag ggaaaatcga cccaatcccg catccgtatg
    22681 gcgaagattt gccgtgtgcg gataacaaac ctgttgcgcc gaaaaagcag gaagcgaaag
    22741 ctgtaatcgt gcagccaccg cgccagaaac cgtgggaaaa aacctacgta ttgctgcctt
    22801 ctttcgagaa agtgaagggc gataaagtgc tgtacgccca tgcttcgcgc attctgcacc
    22861 acgaaaccaa cccaggctgc gcccgcgcat tgatgcaaaa acacggtgac cgctatgtgt
    22921 ggatcaaccc gcctgctatc ccgctttcta ccgaagagat ggactgcgtt tttgcgctgc
    22981 cgtacaagcg cgtgccacat ccggcctatg gcaatgcccg tattccggct tacgaaatga
    23041 tccgtttttc ggtcaacatt atgcgcggct gctttggcgg ctgttctttc tgttctatta
    23101 ccgagcacga agggcgcatt attctgagcc gttcggaaga ttcgatcatt aatgagatcg
    23161 aagcgatccg cgacaccgtt ccaggtttta cgggcgtgat ttccgatctc ggtgggccaa
    23221 ctgccaacat gtatatgttg cgctgcaaat cgccacgcgc tgaacaaact tgtcgccgtt
    23281 tgtcgtgcgt ttatccggat atttgtccgc acatggacac taaccacgaa ccgacgatca
    23341 acctctatcg ccgtgcgcgt gatctgaaag gcattaaaaa aatccttatc gcctcaggcg
    23401 tacgttatga catcgccgtg gaagatccgc gctatatcaa agagttggcg acccatcacg
    23461 tcggcggtta tctgaagatt gctccggaac ataccgaaga agggccgttg tcgaagatga
    23521 tgaagccggg catgggcagc tatgaccgct ttaaagagct gttcgatacc tactcgaaac
    23581 aggcaggtaa agagcagtat ctgatcccgt atttcatctc tgcgcacccc ggtacgcgtg
    23641 atgaagatat ggtgaatttg gcgctgtggc tgaaaaagca tcgtttccgt ctcgaccagg
    23701 tacagaactt ctacccatcg ccgctggcga actcaaccac catgtattac accgggaaaa
    23761 aaccgctggc gaagattggt tataagagcg aagacgtctt cgtaccgaag ggcgacaaac
    23821 agcgtcgttt gcataaagcg ttgttgcgtt accacgatcc ggcaaactgg ccgttaatcc
    23881 gccaggcgct ggaagcgatg ggcaaaaagc atctgattgg cagccgtcgc gattgcttag
    23941 tgcctgcgcc aaccattgaa gagatgcgtg aagctcgtcg ccagaaccgc aatacccgtc
    24001 cggcgttgac taaacatacg ccgatggcga ctcagcgtca gacgcctgct acggcaaaaa
    24061 aagcgtcgtc tacgcaatct cgtccggtga atgctggtgc gaagaaacgc cctaaagcgg
    24121 cggttggacg ttaaaaaaaa tccccggcaa ctgacatgct gccggggatt tttttatcat
    24181 tctgcgtaca tacgcaagca gcgcaggcaa tcaaaatagt cctgtgcatc aagacgcgta
    24241 ttggctctgt tagcttcgga tttggcagca tagatttgtg ggcgtagtgc tttcatatcc
    24301 tgctcaagcc gggcgatttt ggcatttaat gtttgtacat tgctcgccag ttgattgacc
    24361 ttttgctcat tgacgcagcc actgagcaag caggttgcca gaacaacagc tcccacggtg
    24421 aaaatcgttt tcatatagtg ttccctttta tttattgttc cggctaattg ttatttaatt
    24481 cagctcattg ctttaatctg gcggcggttt ttgcctcaat cagcttaagt aggctgccgc
    24541 tgttcgggcg gataaaatca gcgatttccc acttaccttc gcgccgctgt aaaaccagct
    24601 cttgctcttc attgttgcca ttggtcagcg taaaacgaac atgaatgcgg gcggaatcgg
    24661 cggttttctc caggactcga atttcatcaa cgcgtgaaaa tagcgactct tcgccaaaac
    24721 agatcggatt gtcgtaaata aattgccgtt ctatttgcat atcttcatcg ggattattgg
    24781 ccgtcttgaa ggtggtttta tagaaggcat taaccgcctg gcggtaagag tcggaaacca
    24841 cccacgtcca cggccagcta taatcagcaa tatgttcaaa gagttcagca acaaaggatt
    24901 ctggctgttc tttctgcaac gaagctaaag cggccagcgt tttttcattc tcgctattaa
    24961 ctgcttgtaa gacgctgcca tgattgctga caatatcatc aatgacccaa cgaccatttt
    25021 ccgccaccat tttcagtgtc tgtgtggtct tttctttatc atctttaaag atacgaaagc
    25081 gcacaacggc atcggcatga tcgacgtcag tttgggttat cgctacgctt tctaacacca
    25141 gatcgccaaa atcctgacaa tcacaaaccg gatcataatc cagccagcca atattgcccg
    25201 gtagtgtaag attgtcgttc agggtaagcg cctgttgaat acgcgctgaa gttatcgccc
    25261 gctctccggt ttcaccgaaa taaggcgtac tggcatctga tttatagttc tgataaatct
    25321 gccggactgt ttgttctacg gtctgtgaag gaggctgcgc gtgtacggta aaacttgcca
    25381 gggctgctaa aaacagaagt attattttca tctgtttttc acctctccgt tgcgtttatc
    25441 ccggatatag atgagttgtt cttttaagag cttccggctc tgcatgatga tgtccttata
    25501 tttggcattc ctgaacaagg aagagtaaca acgggcggga cgcgagggga ataaatgatt
    25561 tctgaaaagt ccggtagcgg aacattaccg ccaccgggag aatttgcacg ttagccgccg
    25621 aactggtcag gatcgggacc gagacgcttg ccctgatcga gttttgcaat tttgccgagt
    25681 tcgtctttgt cgagacggaa atcccagaca tcaaagtttt cggcaatacg tgaaggtgtg
    25741 accgatttcg ggatcaccac caggccgcta tccagatgcc agcggataac aatctgcgcc
    25801 ggggttttgc cgtatttatc tgccagatcg cgaatgactt tctgatcgaa aacgcctttt
    25861 cctccttgcg ctaatgggct ccaggattcg gtctggattt tgtgtgtcgc gttccaggcg
    25921 tgtagctggc gttgttgcat cagcggatga agttcgatct ggtttatcac aggcgtcacg
    25981 ccagtttcat caatcaggcg ttgcaggtga tggatctgga agttgcacac gccgatgctt
    26041 ttgattaatc cctctttttg caattcgatc atgcctttcc atgcttcgac ataatggtcg
    26101 atagcgggaa cgggccagtg cattaagtag aggtcgatat aatcaagctg gagttttttc
    26161 aggctgtcga gcagggcttc gcgggggcac ttgtggtcgt cgttccacag cttagtggtg
    26221 atgaacagtt cttctctgtt gactgaggca tttttcaggg ctttgccgac accttcttcg
    26281 ttcttgtagg ccgcggcggt atcaatcgag cgataaccca cttctaacgc tttttgaatg
    26341 gcggtgatta cttcctcatt acttgcttgc cagacgccca gtcccagctg gggcatgaca
    26401 ttgccatcct gtagcttaat aacggttgga ttagccatac gttcctcctt tatatgaact
    26461 caccggagca ggctccggtg aggtgttgtg aacttaagtc tggacgaaat gcccgaaaac
    26521 gaaagtttga ggcgtaaaaa gcttagcggg cgactttgta tatacggcgg ctgacatcca
    26581 acgtaatgtc atgattttcg cccagttggg tcatgccgtg ctcttccagt tttttcagca
    26641 aagccgggat ggagctgccg tccagaccgt agtcggagag gtgggtcggc acgcctaatt
    26701 gctcaaagaa attgcgggtt gcggcaatcg cggcgtcaat acgctcatcg tcggaacctt
    26761 cagtgatgtt ccagacgcgt tcagcatatt gcagcagctt agcgcgcttg gtctcgcgtt
    26821 tttcattcca cagtgcaggc aggacgatag ccagtgtttg cgcgtgatcc agaccgtgca
    26881 tcgcagtcag ttcgtggccc agcatatgcg ttgcccagtc ctgcggtacg ccagcgccaa
    26941 tcaaaccgtt cagcgcctga gtcgccgccc acatgacgtt ggcgcgcaca tcgtagtttt
    27001 ctggctcttt cagggctttc ggaccatctt cgattagcgt cagcaaaatg ccttctgcga
    27061 aacggtcctg aattttggca tcaaccggtt tggtaacata ctgttccacg gtgtgtacaa
    27121 aggcgtccac tacgccgtta gccacctgac gcggcggcag ggtgtaggta taaaccggat
    27181 cgagcacggc aaataccggc tgaacatgtg cagaatggaa cgcctgcttg tcgcctgtgg
    27241 ttttacggga gatcaccgcg cctgcgttgg attctgaacc ggttgctggc agcgtcagca
    27301 cacagcccat cgggatggcg cttttaatct ctttaccgcc cgtttgcaga atgtgccacg
    27361 gatcgatatt ttccggatag ttagccgctg cggcgataaa tttggtgccg tccagtacag
    27421 aaccgccgcc aaccgccagc aggaaagtca ctttctgttc gcgaaccagt ttcacggcgt
    27481 tcatcagcgt ttcataagcc gggtttggct caataccgcc aaattccagc acgtccatgc
    27541 ctttcagggc atccagaact tgatcgagaa cgccggtttt tttcacgctg ccgccaccgt
    27601 aggtaatcaa tacgcgagca tcgtgaggaa tttgttcgcg taaaccagcg attgcgcctt
    27661 taccaaacag aatgcgggtt ggggtgtgca gattaaagtt gttcattact tgctcccttt
    27721 gctgggccaa tatgagggca gagaacgatc tgcctgatgt ttttcattgt gatcgccagc
    27781 gccctggctc tcaatgctca tttctgccaa tgtcttgcct atttctccag agtgctggag
    27841 aaatgctaca aaattgcgca caatcaaatt gccgcattat tcctaagaaa ttacgcgata
    27901 tgaaacgtga agagatttgt cgcttgctgg cggataaagt taataaactg aaaaataaag
    27961 aaaatagttt gtcagaactg ttgcccgatg tgcgtttgtt gtatggcgag acgcctttcg
    28021 cacgtacacc ggtgatgtac gagcctggca tcataattct cttttccggg cataaaatcg
    28081 gttatatcaa t
//
LOCUS       DADMFL010000049        27746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.049, whole genome shotgun sequence.
ACCESSION   DADMFL010000049 DADMFL010000000
VERSION     DADMFL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.049"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(57..761)
                     /locus_tag="JNZ27_002070"
                     /pseudo
     CDS             complement(57..761)
                     /locus_tag="JNZ27_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(946..1293)
                     /gene="pspE"
                     /locus_tag="JNZ27_002071"
     CDS             complement(946..1293)
                     /gene="pspE"
                     /locus_tag="JNZ27_002071"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY8664405.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1336..1557)
                     /gene="pspD"
                     /locus_tag="JNZ27_002072"
     CDS             complement(1336..1557)
                     /gene="pspD"
                     /locus_tag="JNZ27_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY8664406.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRPANKLAQRYKR"
     gene            complement(1566..1925)
                     /gene="pspC"
                     /locus_tag="JNZ27_002073"
     CDS             complement(1566..1925)
                     /gene="pspC"
                     /locus_tag="JNZ27_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY8664407.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1925..2149)
                     /gene="pspB"
                     /locus_tag="JNZ27_002074"
     CDS             complement(1925..2149)
                     /gene="pspB"
                     /locus_tag="JNZ27_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY8664408.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2203..2871)
                     /gene="pspA"
                     /locus_tag="JNZ27_002075"
     CDS             complement(2203..2871)
                     /gene="pspA"
                     /locus_tag="JNZ27_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY8664409.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3038..4015
                     /gene="pspF"
                     /locus_tag="JNZ27_002076"
     CDS             3038..4015
                     /gene="pspF"
                     /locus_tag="JNZ27_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY8664410.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4135..5400)
                     /gene="puuE"
                     /locus_tag="JNZ27_002077"
     CDS             complement(4135..5400)
                     /gene="puuE"
                     /locus_tag="JNZ27_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY8664411.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5438..6718)
                     /locus_tag="JNZ27_002078"
     CDS             complement(5438..6718)
                     /locus_tag="JNZ27_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8664412.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRSGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6720..8207)
                     /gene="puuC"
                     /locus_tag="JNZ27_002079"
     CDS             complement(6720..8207)
                     /gene="puuC"
                     /locus_tag="JNZ27_002079"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY8664413.1"
                     /translation="MNFYHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8357..8914)
                     /gene="puuR"
                     /locus_tag="JNZ27_002080"
     CDS             complement(8357..8914)
                     /gene="puuR"
                     /locus_tag="JNZ27_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY8664414.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8941..9705)
                     /gene="puuD"
                     /locus_tag="JNZ27_002081"
     CDS             complement(8941..9705)
                     /gene="puuD"
                     /locus_tag="JNZ27_002081"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY8664415.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9917..11335
                     /locus_tag="JNZ27_002082"
     CDS             9917..11335
                     /locus_tag="JNZ27_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY8664416.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASLGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11484..11648
                     /locus_tag="JNZ27_002083"
     CDS             11484..11648
                     /locus_tag="JNZ27_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664417.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11638..13023
                     /gene="puuP"
                     /locus_tag="JNZ27_002084"
     CDS             11638..13023
                     /gene="puuP"
                     /locus_tag="JNZ27_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY8664418.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPVINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13157..13402
                     /locus_tag="JNZ27_002085"
     CDS             13157..13402
                     /locus_tag="JNZ27_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY8664419.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13715..15358
                     /gene="sapA"
                     /locus_tag="JNZ27_002086"
     CDS             13715..15358
                     /gene="sapA"
                     /locus_tag="JNZ27_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY8664420.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNDSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15355..16320
                     /gene="sapB"
                     /locus_tag="JNZ27_002087"
     CDS             15355..16320
                     /gene="sapB"
                     /locus_tag="JNZ27_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY8664421.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSGSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16307..17197
                     /gene="sapC"
                     /locus_tag="JNZ27_002088"
     CDS             16307..17197
                     /gene="sapC"
                     /locus_tag="JNZ27_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY8664422.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17197..18189
                     /gene="sapD"
                     /locus_tag="JNZ27_002089"
     CDS             17197..18189
                     /gene="sapD"
                     /locus_tag="JNZ27_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY8664423.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18191..18997
                     /gene="sapF"
                     /locus_tag="JNZ27_002090"
     CDS             18191..18997
                     /gene="sapF"
                     /locus_tag="JNZ27_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY8664424.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19065..19418
                     /locus_tag="JNZ27_002091"
     CDS             19065..19418
                     /locus_tag="JNZ27_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY8664425.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACGSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19786..20574
                     /gene="fabI"
                     /locus_tag="JNZ27_002092"
     CDS             19786..20574
                     /gene="fabI"
                     /locus_tag="JNZ27_002092"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY8664426.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20718..21845
                     /locus_tag="JNZ27_002093"
     CDS             20718..21845
                     /locus_tag="JNZ27_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY8664427.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21913..23847
                     /gene="rnb"
                     /locus_tag="JNZ27_002094"
     CDS             21913..23847
                     /gene="rnb"
                     /locus_tag="JNZ27_002094"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY8664428.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGPNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23925..24027)
                     /locus_tag="JNZ27_002095"
                     /pseudo
     CDS             complement(23925..24027)
                     /locus_tag="JNZ27_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24081..26055
                     /gene="pdeR"
                     /locus_tag="JNZ27_002096"
                     /pseudo
     CDS             24081..26055
                     /gene="pdeR"
                     /locus_tag="JNZ27_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26203..26376
                     /locus_tag="JNZ27_002097"
     CDS             26203..26376
                     /locus_tag="JNZ27_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664429.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26466..27215
                     /locus_tag="JNZ27_002098"
     CDS             26466..27215
                     /locus_tag="JNZ27_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8664430.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27484..27702
                     /gene="osmB"
                     /locus_tag="JNZ27_002099"
     CDS             27484..27702
                     /gene="osmB"
                     /locus_tag="JNZ27_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY8664431.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6444 a         7158 c         7133 g         7011 t
ORIGIN      
        1 aacatccgtt ttgtggtgtt tttttaatct ttttggggtt ttaattccta tcgatggaca
       61 tactcagcac ctttctcaag gtaacagagt aaaacatcca ccatcgccag caacacttca
      121 gggctacggt aattcaggtc aatttgatcg tcactaaagg tggtccataa atggcgcgtg
      181 gaattatcat ccatttgaaa cggcgttaat agcggtaacg cacgcggacg ggtgacggca
      241 ctgagatcgg ttttcggatc aaccgcaata aagaaatctt caaaacccgg aagctgttgt
      301 aaatagtttt taaaccattc actttttgca gacatatggt tgcagacaaa atcaaacatt
      361 aaatgactgc attcaccgag ttgctgaata tcctgccact cccccgcttc actggcgacc
      421 tgatgataat caattaccga aaatccatca tcagatgacc atgggtaaaa aggtaataaa
      481 tgaacatgtg aaaaaatgct ttgcagccat tgatggtaga actgattaaa tatgggtagt
      541 ggttttaaat cattactgtg aaattgatcg gcataggtaa ttaacacgac atcactttca
      601 tcccagtgtt ttttacgtcg ctgtgtaatt aatcgtttcg cactctcaag acgcgttaca
      661 agtttctgta aatgagttgc ggtaaatgtt ccaccgtaga tttcgtccag gtaatccgta
      721 attttctgtt tcatgttgcc tttccgtggt agcggtccca tgaggcgaga gactactccg
      781 cagaaaataa actgtcaatt gagcacaagg gttttagtca cgcaggaaat cattttttac
      841 cagggaaaaa gcgtatttgt gagctgacgc aaataatcta tagggatgta aataaggtgt
      901 gacagaaaaa aaacggcgca taagcgccgc tcatggtgaa ttcttttaac ctttgacctt
      961 cggcattgcg atgtctttca ggccaccggc gttctcaacg tgggtatatc ccatctcgct
     1021 aaggatctct tttgcctgcc cggactggcg tccggcattg caatacactt tcacggtgtc
     1081 gtttttatcc ggaacggcgg tggcaatgcg ctctttcact tccttcaggg gaatattgat
     1141 ggccccctga acgtgctctt gctgatactg ctctggaaca cgaacatcga tccagtgttc
     1201 agcggcgaaa acgggcagtg aaaacaccag tgccagagca agtaagcctt tttttaaaca
     1261 tgtaagcgtc cttctatgga tgaaaattgc catccattat acgcctgaga tttaacaatt
     1321 tttgcgaaac tcccctcacc ttttataacg ctgtgccagt ttattagcag gccgacttaa
     1381 cagcggttcc agcgccacag ccagcaacat tttcagcggt cggcgagcaa ctgattttat
     1441 cgcccagccc gccacacccg ccgggccata gcgcagtgcg gtaagaagta ccagcttgcc
     1501 tgctaatttg aaaccaggct tcaccttttg cccggcctgt tgccagcgag tattcataac
     1561 tttcctcaca gttgacggaa acggctacgt aacgtgaaag tatcggaagt gacataacgt
     1621 tccatctcgc gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc atccagcaat
     1681 tcgctgctgg aaggtagctg ctcaccaaag gccatgttgt ccggcattgg atcaagcgca
     1741 aatgacaaaa tgatgtaagc aaccagggta aacagcgcca gaccgaagaa aatcgacagc
     1801 accaccagga tacgcaccag ttttaccggt acatcaaaat agttggcaat cccggcgcag
     1861 acgccgcgga ccatgccctg ctgtggaata cgccataatt ttttattgag attaatgccc
     1921 gccattagcg atccctccag ttcggatgtt ctgcatcaag aatagattcc agcgcctgaa
     1981 tacgttcgcg catccgtttt gcttcatcaa ccagttgcgc taatcgctgc tgctcacttt
     2041 gcgacaattc actgcgacca gaacgattgc tgtaatgcag ccataaccag atcggtaaaa
     2101 caaacagcac aaaaatggtt aacggaatag ccagaaatag cgcgctcata agtactcctt
     2161 agacgatgag cggcggcgcg ttcggacgcc gcctggatgt tattattgat tgtcttgctt
     2221 cattttggct tttaattgtg ccagttgttc gctgattgca tcatcggctt tcagttcggc
     2281 aaactgatcg tccagcgatt tttgtttacc gaagctgtgg ctttctgctt ccgcttccat
     2341 ctggtcaata cgacgttcga aagattcgaa acgagccatt gcttcatcca gtttgccact
     2401 gtccagctga cgacgcacat cgcgcgacga gtttgccgcc tgatgacgta acatcaatgc
     2461 ctgctggcga gcgcgtgttt cgctcaattt gttttccagc tcgccaatct ctttcttcat
     2521 gcgtgccagc gtatcgtcca ccagcgtcac ttcatgttcc agggacttaa tcagatcggt
     2581 cagtttctgt ttttcaatta acgctgcacg tgccagatcc tctctctctt tcagcagcgc
     2641 cagttcggct ttttcctgcc attcaacttc acgcgccgac gcttgttcaa tacggcgagt
     2701 cagctgtttc ttttctgcca acgcacgcgc cgaagtagaa cgtacttcaa ccagtgtatc
     2761 ttccatctcc tggatcatca gacgcaccag tttctgtgga tcttccgctt tctctaacag
     2821 agcgttgatg ttggcattca cgatgtcggc aaagcgagaa aaaataccca taatgttgtc
     2881 ctcttgattt ctgcgttcag gattgtcctg ctgaactgtt aatacaattt gcgtgccaat
     2941 tttttatctt tttgatttat aaagatctga ttgaagaatc aacagcaaca tgccaggatg
     3001 aattagcgaa ttacactaac aagtggcgaa tttcatcatg gccgaataca aagataattt
     3061 acttggtgag gcgaacagct ttctcgaagt gctggaacag gtttcgcatc tcgcaccgct
     3121 ggacaaaccg gtgctcatca tcggcgaacg cggcaccggt aaagagttga ttgccagccg
     3181 cctgcattat ctctcctccc gttggcaagg gccgtttatt tcccttaact gcgcggcgtt
     3241 aaatgaaaat ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt ttaccggtgc
     3301 gcaaaaacgt catcctggga gatttgaacg tgccgacggc ggtacgctat ttcttgatga
     3361 actcgctacg gcaccgatga tggtgcagga aaaattattg cgtgtgattg agtacggtga
     3421 actggagcgc gttggcggta gccagccgtt gcaggtgaat gtgcggttgg tatgcgcgac
     3481 gaatgccgat ctcccggcga tggtcaatga aggtactttt cgcgctgacc tgctcgaccg
     3541 gctggctttc gatgtagtgc aactgccgcc cctgcgcgag cgcgaaagcg acattatgct
     3601 gatggcagaa cactttgcca ttcagatgtg tcgggaaatc aagctgcctc tgttcccggg
     3661 gtttacggag cgcgccagag aaacattgct gaattatcgt tggccgggaa atattcgtga
     3721 attgaaaaac gtggtggaac gttcagtgta tcgccacggc accagcgatt atccgcttga
     3781 tgacatcatt attgatccct ttaaacggcg tccacctgaa gaagccatcg ccgtttcaga
     3841 aaatacctcg cttccgacgc tgccgctgga tttacgcgag tttcagatgc agcaggaaaa
     3901 agagttgctg caactcagtt tgcaacaggg gaaatataac cagaagcgcg cggctgaatt
     3961 actggggtta acttatcatc agttccgcgc gttgttgaaa aagcaccaga tttagcgaag
     4021 ttgagcaata caacttgccg gatgcggcgt gaatgcttta ttcggcatac ataatcaata
     4081 cgtcgtttgc aggcctgata agcgcagcgc atcagacatt attgcgttgg gctattaatc
     4141 gctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg ggatggtcag
     4201 cggatacagg aagcgaatca cgttgccgta tgcgccacag gtcagcagga gcagcccctg
     4261 cgccagcgcg cgttgctgga ttttctgtgc aatcgccgct gacggctcgc ccgtttgcgg
     4321 atcgttaaac tctgccgcaa tcatcgaccc caggccgcgt accgcagcaa tggccggaac
     4381 gctttctttg gcatcaatca acgtgttttt gagacgctgg cccagttgat tcgcgcgttc
     4441 gcagagtgat tctttgtcga taatattgag caccgcgtgc gcggcagcca ccgccagcgg
     4501 gttcccggcg taggtgccac ccaacccgcc cggcgcgggc gcgtccataa tattcgcgtt
     4561 accgaccacg cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg tcattaagtc
     4621 cggcttatcg gcgtaatgat ccatggcaaa cagtttaccg gtacgcgcaa agccgctttg
     4681 cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa tggcggcaac
     4741 cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga aaataatggc
     4801 cgcgacctgc ttcgcttcga tgtctgattt aaacaagcgt tcgatggcgt cgagggagtc
     4861 ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg aaccagggaa
     4921 cgggccgaag ccgattttgt acggcgcaac ttttccggtc agcgccatgg tcatatatgt
     4981 acgaccgtgg aagccaccgc taaacgcaat cacgccaggt cgtccggtat gggcgcgagc
     5041 aattttcacc gcgttttcca ccgcttccgc gccggtagtg aagaatgcgg ttttagcctg
     5101 cccgctcact ggggcaaggg cgttaatttt ctccgccagc gtgacgtagc tttcgtacgg
     5161 cacaatctga tacgccgtgt gggtaaactg ttgcaattgc tgctccaccg ccgcgaccag
     5221 atcagggtgg cgatgcccgg tattcagcac cgcaatgcct gcggcgaaat cgatgtattc
     5281 gttgccctct acgtccttca gcgtagcgtt ttctgccgac tgggcgaaga agttacacat
     5341 cacgccaacc ccgcgtgggg tagcagaaag acgacgctga tggaattcat tgttgctcat
     5401 gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga cgatcgcgca
     5461 ggctgtaata agccgcgccc atcgcggtaa atggcacgcg cagcgtgcgc ccgccgggga
     5521 acgggtaatg cggcagattg gcgaaggcat cgaaacgctc ggcgtcaccg cgcagcagtt
     5581 cagcaatcaa acgcccggca agatgggtac aggtgacgcc gtggccgctg taaccctgca
     5641 tgtaatagat gttggtatca aggcggccga actgcggcat gcgcgacaag gtcagcagga
     5701 agttgcccgt ccagcggtaa tcaattttca cgcccttcaa ctgagggaag gtttttagta
     5761 gtttcggtac cacaagacgc tcaacgtcat ccgggttgcg tgcaccatac actacacccc
     5821 cgccgtacag caggcggttg tcggcggtaa gacggtagta atcaagcaga tagttacagt
     5881 cttccacaca gtagtttttc gggatcagcg aacgggctaa atcttccgac agccgttcgg
     5941 tggtgatcac ctgcgtgccg cacggcatgc tgcgtttcgc tagttccggc tctactttat
     6001 cgcccagata cgcattcccg gcgacaatca catacttcgc cgtcacctga ccattggcag
     6061 tccgaaccac ggctggcgtg gtgtgctgga tctgcgtcac ggcggaaagc tcatacacgc
     6121 gcccgccgtt caggcggatg gcatcggctt caccaatggc gaggtttagc gggtgaatat
     6181 gcccaccgct gtgatccagc agcgcaccag tgtagcgatc gctggcgacc tcgcggcgaa
     6241 tagcgttggc gtccagcagc tccagctgtt tattgccgta gcgttcccag ttctctttct
     6301 gctcttcgag tgtggcgagt tgtttatcat tcatcgccac aaacagaccg ccggggcggt
     6361 agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcgcca ccctcgaaca
     6421 tcatgctgcc gagcatacgg gcggtgtcca tgccgtagct tttttcgatc acgtcaatgt
     6481 cgcggctgta ggagttcaca agctgcccgc cactgcgccc gcttgcgcca aagccgatgc
     6541 gtgaggcttc gagaaccact acgtcaaagc ccgcttccgc cagatgcagc gcggaggaga
     6601 gcccggtata gccgccgcca accacgcaaa cgtcgcaggt gattgactca ttcagtgtgt
     6661 cgaatggtgc atatttattc gcactggcgg cgtagtaact gctggtatgt tcggtcattt
     6721 caggcctcca ggcttatcca gatggttttc agttcagtga atttttcaag ggcatgcagg
     6781 gatttgtcgc gaccgttgcc gctctgctta tagccgccaa acggcacggt catatcgccg
     6841 tcgttgtagt tattgacgaa gacggaaccg gctttcaggc gtcggctcat gcggtgcgcg
     6901 cgggagaggt cgcgcgtcca taccgccgcg ccaaggccgt actggctgtc gttggcaagc
     6961 tgcagcgcct gatcttctga tgtgaaacgg gtgaccacca gcaccggacc gaaaatctct
     7021 tcgcggctta aggacgcatt cgggtccaca tccacgaaga tggtcgggcc gatggccgct
     7081 gccagcccgg cgttgcgacc atccagcagc agttgcccct tgccttcgcc ttcacgaata
     7141 aaggtgtgta ccgagtcagc gtgagcgctg tcgattaagg tgcccatggt ggttgcggga
     7201 tcaagtggat ggcccggctg ccagttttgc gcctgctgtt ttaacagggc taagaattca
     7261 tcggcgatgc tctcttccag taacaaacgc gttccggcga tgcacacctg gccctggttg
     7321 tagaagatgc ctgcggcggt ggcgcttgcc gctttttgca gatccggaca atcggcgaaa
     7381 acgatgttgg cgcttttgcc gcccgcttcc agccagacac gtttcatgtt gctatcaccc
     7441 gcgtctttca gcagttgttt acccgtacgc gtggagccgg taaaggcgat ggcatcgata
     7501 tcgttatgac gcgaaagcgc ctgccccgct tcatgaccaa aacccgtcac cacgttcaac
     7561 acaccatccg gcaagcctgc ttctttcgcc agccccgcga gacgaatcgc actgagcggt
     7621 gatttttcag acggttttag aatcacgctg tttcccgccg ccagcgccgg gccgagtttc
     7681 cagcaagtca gcaacagcgg gaagttccac ggcacgatgg cggcaatcac gccgaccggt
     7741 tcacgcacga tcatcgccag ctcatggcta ctggtggtcg ccacttcgcc atacactttg
     7801 tcgatcgctt cggcgtacca gcgaatggcg cgcgccgcgc cgggaatatc atcacgcaga
     7861 ctgtgacgaa tcggtttgcc ggtgtcgaga gtttccagca gtgccagctc ttcggcgtgg
     7921 gcttccatta aatcggcgag tttattcagt accgctttac gtttagccgg agaagagagt
     7981 gaccagtcgc cgcgttcaaa tacgccgcga gctgcgctca ccgcacggtc gatatcgacg
     8041 ctcttgccgc gggcaatttt cgccagcggt gcctgggtga ccggatcaac ggtttcaaag
     8101 gtttcatttt ccgccgcagc agtatattca ccgttaataa ataagcggtt ttcaatggcg
     8161 agacttaacg ctttatcctg ccagtaagcc agatgataaa aattcattat gactcctgtt
     8221 tcacgtctat cagatatatg cagtttgtga acgtcagggc gcagagatct ccgggctgca
     8281 tgcccgttta tttctggcga cattctttat ggattgccgg gcgcattgcg cttacccggc
     8341 ttacagaaca aaaagattaa aacgtggtgg gcgtatgggc gctgataatt cggcaaatac
     8401 ctgccgacgt attactgaaa ctgtgcggga tgccggtatt aatggcataa ctttgccccg
     8461 cgacgaggtg gtaatcctga ccattaatcg tcagaacaat ttcaccttcc agtactgtgc
     8521 ctatttcctc accctgatgc ttaattcttt ccccagtggt tgtgcccggc tggtacgttt
     8581 caaagatcat cgccagcgtg cgattcgggt taccgttatg aaccagcttc attgacacac
     8641 cctgactgcc catctcaatt aagtcgtcct gattaatgac gacctgcggc tcatcaggtt
     8701 tttccggctc ggaaaagaat tccgagagtg acagaccata caccttcagc agcttttgca
     8761 gcgtactgat ggcagggctg actttatctt gttctatcgt actgatagca ctgtgagtca
     8821 gcccggagag ttcggcggca cgacgttgtg aaagcccctg ctgctggcgg atttccgaca
     8881 agcgttttcc tggcgccagt ccctcatcac tcatatttgc atttccttaa actgtagtgg
     8941 tcagagtcgc tgtttttcag cgatatggtg ctgccaagcg gtgataaagc cctcgaacaa
     9001 tatacgcgaa agcgcgtact cgctactgtt ccattccggg tgccactgta cgcccagcgc
     9061 aaaaggatga ttgatgacgc taaccgcctc aactaagcca tccggcgagc gagcttcaac
     9121 acgcaaccgt gggctaacga ccttcgcccc ttgtccatgt agagagttta cccaaaagtt
     9181 gctacattca ggtaacaacg cagacagtaa tcccccctct tcaacctgaa cttcatgcga
     9241 cggtgcatat tgctgttcca ccggcagttc gggatcttcc cgatgctcca gcaattcagg
     9301 ctgttcgcac agcttgcgat gcaacgaacc accggttgcc accaccagtt cttgtaaacc
     9361 ccggcagatg gcgaaaatgg ggatgcgcct ttcgagtgcg gcattaatta tcgccatgct
     9421 cagaagatca cgcccgggat cggcgtcagg ctcatcgccg ttttcaccat ataggtgcgg
     9481 ctgcacattg ctgggactac taggaagata aatgccatcg agtttcggca aaagttgttc
     9541 aagtaacgac ggttccgcca gcgcatgtgg tagcgcaatt ggcaagccgc ctgcgtggat
     9601 gatggcattc aggtactttt cttgcagagt ctgggtcgca tgacccttaa gcctgttcct
     9661 gcacattacg acaccgataa ccggattgtt cattatattt tccatgctca atctcacaaa
     9721 gtggactaaa ttatcgccat tactgcgctt taaatgacct ttatgttcaa tattttttca
     9781 acctagcagt ggattttcat ttttgcaaac taaatttaac atttgacaaa catttggttt
     9841 gcatacagat tcgaatgatg gtcattatat tttacgcttt gataacgagc ggaaaacaaa
     9901 ccaaaggcga agaatcatgg aaaccaatat cgttgaagta gagaactttg ttcagcagtc
     9961 agaagagagg cggggtagcg cctttacgca ggaagtgaaa cgctacctgg agcgctaccc
    10021 gaatacgcaa tatgttgatg ttttacttac tgatttgaat ggttgcttcc gtggaaaacg
    10081 tatccctgtt tcaagcctga agaagctcga gaaagggtgt tatttcccgg cctcggtatt
    10141 tgcaatggat attctgggca acgtagtaga agaagcaggt ctgggtcagg aaatgggcga
    10201 gccggatcgt acctgtgttc ctgttctcgg ttccttaact ccttctgccg ccgatccaga
    10261 gtttatcggt cagatgctcc tgaccatggt cgatgaagat ggcgctccct ttgacgttga
    10321 gccgcggaac gttcttaacc gcctgtggca gcagctgcgc cagcgcggat tgttcccggt
    10381 tgtagcggta gagctggagt tctatttact ggatcgccag cgcgacgctg aaggggatct
    10441 gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca caaagccagg tttactccgt
    10501 tgataacctc aaccacttcg ccgacgtgct caatgatatt gatgaactgg cgcagttgca
    10561 gctgattccg gcagatggcg cggtcgctga ggcctcgctg ggtcagtttg aaatcaacct
    10621 ttaccatact gataacgtgc tggaagcctg cgatgatgcg ctggcactaa aacgcctggt
    10681 gcgtctgatg gcagaaaagc ataagatgca cgccactttt atggcgaagc cgtatgaaga
    10741 gcacgcgggc agcgggatgc atatccatat cagtatgcaa aataatcgtg gcgagaatgt
    10801 gctttctgac acagaaggcg aagattcgcc gctgctgaaa aagatgctcg ccgggatgat
    10861 tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac gtgaactcgt atcgccgctt
    10921 ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc cataacaacc gcaccgtcgc
    10981 cctgcgtatt ccgtgcggtg accgtcataa tcaccgcgtg gaatatcacg tggcgggtgc
    11041 cgatgccaac ccatatctgg tgatggcagc gatttttgcc ggtattttgc atggccttga
    11101 taacgagctg ccgttgcagg aagaagtcga aggcaacggg ctggaacagg aaggcttacc
    11161 cttcccgatt cgccagagcg atgccctggg tgagtttatc gagaatgatc acctgcgccg
    11221 ctatttaggc gaacggtttt gccatgtgta tcacgcctgt aaaaatgatg agctgttgca
    11281 gtttgagcgc ctcatcacag aaacagaaat tgagtggatg ttgaaaaacg cgtaattccc
    11341 cttgcggggt tgtttcatcg gcctgagcgg agccaggcct gacatttgct ggtaatccag
    11401 acatttcccg gatgtcgcgt tatggagcgc aggcggcaac gggcttccgt taacgattaa
    11461 gactgtgaca aggaaatgag atgatgccga tgattaaatc cccgcatggc gagggaggct
    11521 gcgtatgcgc ccctcctgct actgactgga ctccgccccc tttgcttccc ttgctaaaca
    11581 ggtttgattt caggtcgaca cgaccgcaaa ccttattacg caggggaggc agcaattatg
    11641 gctattaatt caccactgaa tattgctgcg caacccggca aaacccgtct gcgaaaatca
    11701 ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc tcacgccgat gaccgtattt
    11761 gatacttttg gtattgtctc cggcattagc gacggtcacg ttccggcgtc ctatttgctg
    11821 gcgctggcgg gcgtgctgtt taccgctatc agctacggca aactggttcg ccagtttccg
    11881 gaggccggtt cggcctatac ctacgcgcaa aagtcgatta acccgcacgt cggatttatg
    11941 gtcggctggt catcactgct ggattatctc tttttgccag tgatcaacgt tttgttggcg
    12001 aaaatctatc tctccgccct cttcccggaa gtgccgccgt gggtgtgggt ggtaaccttc
    12061 gtcgccattt taaccgccgc gaatctgaag agcgtcaacc tggtcgctaa cttcaatacc
    12121 ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta tcttcctagt ggttcaggga
    12181 ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc agccgtttat cagcgagaac
    12241 gcgcacctga tcccgattat taccggtgcg acgattgtct gtttctcgtt cctcggtttc
    12301 gatgcggtga ccacgctttc ggaagagacg ccggacgccg cacgcgtgat cccgaaagcc
    12361 atcttcctga cggcggtata cggcggcgtt atctttatcg cggcgtcgtt ctttatgcag
    12421 ctgttctttc ccgatatcag ccgctttaaa gacccggacg ccgcactgcc tgaaattgcg
    12481 ctgtacgtcg gcggcaagct ctttcagtcg attttcctct gcaccacgtt tgtgaacaca
    12541 ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc tgctgtatgt gatggggcgt
    12601 gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc caaaatggcg gactccggca
    12661 ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc tgttctttga tttagtcacc
    12721 gcgacagcac tgattaactt cggtgctctg gtggcgttta ccttcgtgaa tctgtcggtg
    12781 tttaatcatt tctggcggcg taaaggaatg aataaaagct ggaaggatca cttccactat
    12841 ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc tgtgggttaa cctagagtca
    12901 acgtcactga cactcggtct ggtatgggct tcgttaggcg gcgcatattt gtggtatctg
    12961 atccgccgct atcgcaaagt gccgctgtac gaaggtgaca gaacaccggt gagcgaaacg
    13021 taatagccgg atgcgcggcg cattatcctg cctgagggta acgcgccgcg ccattaacta
    13081 taaaaagatg tatgacccgg atcacgagtg ctatcatccg cccttatgct tagcgcatta
    13141 accccagggg caaataatga accacgatat cccgctaaaa tattttgata ttgccgatga
    13201 gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa cgcacaccgc tggcccacta
    13261 cttccagtta ctgctcaccc atttgatgaa caatgaagag atcagcgaag aagcccaaca
    13321 tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt gatgagatcg cagagttttt
    13381 gaatcaatgg ggtaatgagt aattatttaa ttgcagacga tcggtcagta tcgttgtccg
    13441 gataaagcgt tcacgccgca tccggctaac aatgacacgc actgcagcgt gctttaatgg
    13501 gtttctcacg atcgtaccgc tctgctcctc gctaagttgt tctgtgagtt atagttatag
    13561 agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca cctgttcaac gaccaaccac
    13621 acggccctca ttacgacccc tgaacacaaa cggtaatggc aaatgtcatt aaatattagt
    13681 aagatagcct ctttacataa aatcccttaa tattatgcgc caggtattat cgtctctttt
    13741 ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg cctgaatccc ccccgcatgc
    13801 tgatatccgc gacagcggtt ttgtctattg cgtcagcggg caagtcaaca cctttaaccc
    13861 atccaaagcg agcagtgggt taattgtcga tacccttgcc gcccagtttt atgatcgact
    13921 gctggatgtc gatccctata cctatcgcct gatgccggaa cttgccgaaa gctgggaagt
    13981 actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc gatgttccgt ttcaaaaaac
    14041 cgactggttt actcccactc gtaaaatgaa tgccgacgat gtggtgttta ccttccagcg
    14101 aatttttgac cgcaacaacc cgtggcataa cgtcaacgac agcaacttcc cctacttcga
    14161 cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa ctggataatc ataccgttga
    14221 gttccgactc gcccagccgg atgcttcttt tttgtggcac ctcgcaaccc attatgcttc
    14281 ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa gatcgccagg agcaactcga
    14341 ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa taccgcgccg ggcaatttat
    14401 tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg ttaatgccgc aggtagtggt
    14461 ggatttaggc tccggcggca ccggacgtct gtcgaaactc ctgaccgggg aatgcgacgt
    14521 tctggcctgg cctgctgcca gccagctatc cattttgcgt gacgacccgc gcttgcgttt
    14581 aacgctgcgt cctgggatga acgtcgccta tctggcattt aacaccgcca aaccgccgct
    14641 aaataatccc gctgtccgcc atgcactggc actggcgatt aataaccagc gcctgatgca
    14701 atccatctat tatggtacgg ctgaaacggc ggcctctatt ttaccgcgcg cctcgtgggc
    14761 ctatgacaac gaggctaaaa ttactgaata caatccggcg aaatcgcgcg aacagttgaa
    14821 ggcgttgggg ctggaaaact taacgctgaa actgtgggtg cccacacgtt cgcaggcgtg
    14881 gaaccccagt ccactgaaaa ctgccgaact gattcaggcg gatatggcgc aggttggcgt
    14941 aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag gcgcggttga tggatatgag
    15001 ccatgatctg acgttatccg gttgggcgac ggacagtaac gacccggaca gtttcttccg
    15061 tccgttactg agctgcgcgg caattcattc acagaccaac ctcgcccact ggtgcgatcc
    15121 gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag cagctggcag cgcgtattga
    15181 agcctatgac gaagcgcaga gtattctggc gcaggaatta cctattctgc cgctggcgtc
    15241 gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt ctggtactta gcccgtttgg
    15301 taacgcctcc tttgctgggg tgtatcgcga gaaacaggat gaggtgaaaa aaccatgatt
    15361 atcttcacct tacgccgcat tttgttattg attgtcacct tgttcctgct gaccttcgtt
    15421 ggcttcagct tgagctattt cactccacac gcgccgttac aaggcgcgtc actgtggaat
    15481 gcctgggtgt tctggtttaa tggcctgatc cactgggatt ttggtgtttc cagcattaac
    15541 ggtcaaccaa ttgccgaaca gttaaaagag gtcttcccgg cgacgataga actgtgcatc
    15601 ctcgccttcg gttttgcgct gattgttggc atccccgtgg ggatgattgc cgggattacg
    15661 cgccataagt ggcaggataa cttgatcaac gccatcgcct tactgggctt ttcaatcccg
    15721 gtgttctggc tggcgcttct gttgacactg ttttgttcac tcacgctagg ttggttgccc
    15781 gtttcagggc gtttcgatct gctctacgaa gtgaaaccga ttaccggttt tgcgttgatt
    15841 gatgcctggc tttcgggctc accatggcgg gatgaaatga tcatgagcgc aatccgccat
    15901 atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa ctgaagtgat ccgcctgatg
    15961 cgtatcagca ctattgaagt gtatgaccag aactatgtaa aagcggcggc gacccgcggt
    16021 ttgtcgcgct ttactatttt gcgtcgccac gttctgcata acgcgttacc gccggttatt
    16081 cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg cgatgatcac cgaaatggtt
    16141 tttagctggc cgggtttagg gcgctggtta attaacgcca tccgccagca ggattacgca
    16201 gccatttccg ccggagtgat ggtgtgtggc tcactggtca ttattgttaa cgtgatttct
    16261 gatattttgg gtgccatggc taaccctctg aaacataagg aatggtatgc cttacgatag
    16321 cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc gcctggcgca aattttatag
    16381 tgacgcctct gcgatggtcg gcctgtacgg ctgcgcggga ctggctgtac tgtgtatttt
    16441 tggcggctgg tttgcgcctt acggcatcga ccagcaattt ctcggttatc aattattgcc
    16501 gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg gggactgacg acctcggacg
    16561 tgatgtttta agccgtttac tgagcggagc tgcgccaacc gtgggtggcg catttgtggt
    16621 gacgcttgcc gcgacgatct gtggcctggt actgggcacc ttcgccgggg cgacacacgg
    16681 cctccgctca gcggtgctta accatattct ggatacatta ctggcgatcc cttcgctgct
    16741 gttggcaatt atcgttgtag cgtttgccgg accgagtttg tcccacgcta tgtttgctgt
    16801 ctggctggca ttgctgccgc gtatggtacg ttcgatttac agcatggtgc atgacgaact
    16861 ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca tcaacgctga atattctctg
    16921 gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc gagatcaccc gcgcactgtc
    16981 gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat ctcggcgcac aactcccctc
    17041 gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg atttatgtcg ccccgtggac
    17101 tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg ttggttaacc tccttggcga
    17161 tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc cattactcga tattcgtaac
    17221 ctgaccattg aatttaaaac cggtgatgag tgggttaaag ccgtcgaccg ggtaagtatg
    17281 accttaaccg aaggtgaaat ccgcggtctt gttggtgaat ccggttccgg caagagtttg
    17341 attgcgaaag cgatttgtgg ggtgaataaa gataactggc gtgttactgc tgaccgtatg
    17401 cgttttgatg atatcgattt gctgcgtctc tccgcacgcg aacggcgcaa actggttggc
    17461 cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc ttgacccttc agaacgtgtg
    17521 ggtcgccagt tgatgcaaaa catcccagcc tggacctata aaggccgttg gtggcagcgt
    17581 tttggctggc gcaaacgccg tgccattgaa ctgctgcacc gcgtggggat taaagatcac
    17641 aaagatgcga tgcgcagttt tccctatgag ttgaccgaag gtgaatgtca gaaagtgatg
    17701 atagccattg cactggcgaa tcaaccgcgt ctgctgattg ctgacgaacc gacaaactca
    17761 atggagccaa caacccaggc gcaaatcttt cgcctgctga cgcgtctcaa ccaaaacagc
    17821 aataccacta ttttgcttat cagccatgac ttacaaatgc ttagccaatg ggcggataaa
    17881 attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc cgagtaagga gttggtgacg
    17941 atgccacatc atccttatac ccaggcgctg atccgcgcga taccagactt cggcagcgcg
    18001 atgccgcata aaagtcgcct caatactcta cccggcgcta tcccattgct ggaacagtta
    18061 ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac aacgagaatg cattgtgacg
    18121 ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc atttcccgct gaacatggag
    18181 aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat ttaagtaaaa cgttccgcta
    18241 ccggaccggt tggtttcgtc gtcagaccgt agaagcggta aaacccttga gctttacgct
    18301 acgtgaaggc cagacactgg cgattattgg cgagaatggt tcgggtaaat ccacactggc
    18361 aaaaatgctg gcgggaatga tagaacctac cagcggcgag ttattgattg acgatcatcc
    18421 gctgcatttt ggcgattatt ccttccgtag tcagcgtatt cgcatgattt ttcaggatcc
    18481 ttcgacctcg ttgaatcccc gtcaacgtat ctcacaaatt ctggattttc cactgcgcct
    18541 gaacaccgac ctggaaccgg aacagcgtcg caaacagatt attgaaacga tgcgtatggt
    18601 tgggctgcta ccggatcacg tcagttacta tccgcatatg ttggcacccg gacaaaaaca
    18661 gcgtctgggt ctggcgcgcg cgctgatatt gcgcccaaaa gtcattattg ccgatgaagc
    18721 cctcgcttca ctggatatgt cgatgcgttc gcagttgatt aatctgatgc tggagttaca
    18781 ggaaaaacag ggcatttcgt atatttatgt tacccagcat atcggaatga tgaagcacat
    18841 tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc gagcgaggca gcaccgcgga
    18901 tgtgctggca tcgccgctgc atgaactcac caaacggctg attgccgggc actttggcga
    18961 agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt tctgaatcct ccttccagat
    19021 tattcacctc attctgacaa gagattaaat cttgtcagaa tgaggtgatg gataaaataa
    19081 aatcaaatgc gcgtgattta agacgcaatc tcactttgca ggaacgaaag ctctggcgat
    19141 atcttcgcag ccgacgtttt ggtgatttca aatttcgccg tcaacatccg gtggggagct
    19201 acattctcga ttttgcttgc ggctcggcgc gcgtagtcgt tgagctggat ggtgggcagc
    19261 atgatttggc agttgcctat gataccaggc gcactagctg gcttgagtcg cagggctgga
    19321 ccgtgctgcg tttctggaat aacgagattg attgtaatga ggaggcggtg ctggagatta
    19381 ttctgcagga actgaaccgc cggtcaccct caccctgaaa gggcgagggg gccgaccgag
    19441 ccgaatagct gttgtggtga aaacatggag acagtgctgg agaatattcg gcaaggtctg
    19501 aaccatccca gccatcgcca taaaagggtt aggggctgta tgagcctgtt tgttgctggg
    19561 gtaacaatat ttgcacaata tggtcccctc gcccctctgg ggagagggtt agggtaaggg
    19621 gaaaagcgcc ccccctgccg cagcctgctc cggtcggacc tggcaactat agctactcac
    19681 agccaggttg attataataa ccgtttatct gttcgtactg tttactaaaa cgacgaatcg
    19741 cctgattttc aggcacaaca agcatcaaca ataaggatta aagctatggg ttttctttcc
    19801 ggtaagcgca ttctggtaac cggtgttgcc agcaaactgt ccatcgccta cggtatcgct
    19861 caggcgatgc accgcgaagg agctgaactg gcattcacct accagaacga caaactgaaa
    19921 ggccgcgtag aagaatttgc cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt
    19981 gcagaagatg ccagcatcga caccatgttc gctgaactgg ggaaagtttg gccgaaattt
    20041 gacggtttcg tacactctat tggttttgca cctggcgatc agctggatgg tgactatgtt
    20101 aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca tcagctccta cagcttcgtt
    20161 gcaatggcaa aagcttgccg ctccatgctg aatccgggtt ctgccctgct gaccctttcc
    20221 taccttggcg ctgagcgcgc tatcccgaac tacaacgtta tgggtctggc aaaagcgtct
    20281 ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac
    20341 gccatctctg ctggtccgat ccgtactctg gcggcctccg gtatcaaaga cttccgcaaa
    20401 atgctggctc attgcgaagc cgttaccccg attcgccgta ccgttactat tgaagatgtg
    20461 ggtaactctg cggcattcct gtgctccgat ctctctgccg gtatctccgg tgaagtagtc
    20521 cacgttgacg gcggtttcag catcgctgca atgaacgaac tcgaactgaa ataatcgttc
    20581 tgttggtaaa gatgggcggc gttctgccgc ccgttatctc tgttatacct ttctgatatt
    20641 tgttatcgcc gatccgtctt tctccccttc ccgccttgcg tcaggataac gatttccttt
    20701 acgaccaagg agcgcccatg gaacaacgcc acatcaccgg caaaagccac tggtatcatg
    20761 aaacgcaatc cagtactacg gagtatgacg ttctgcctct ggtcccggaa gccgcaaagg
    20821 tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga gacgctggcc cccttccttt
    20881 catggctggt ccctgcgcgc gttcttgcag tggagttgtt ccctgaccag cttaccgtga
    20941 cccgttcaca aaccttcacc gcttatgaac gcttgtcgac ggccctgacg gttgctcagg
    21001 tttgcggcgt ccagcggtta tgtaactact attcggcgcg acttacgccg ctccccggac
    21061 ctgattcctc cagggaaagt aatcatcggt tggcacaaat cacgcaatat gcccgccaac
    21121 tggctagctc gcctgctatt atcgacaacc gatcgcgcca gcatctgaat gacgtcggtc
    21181 ttactgtctg ggactgtgtg atcattaacc aaatcattgg ttttattggc tttcaggcgc
    21241 ggacaattgc gacatttcag gcttatctcg ggcatccggt acgctggtta cccgggctgg
    21301 cgatacaaaa ctacgccgac gcgtcactgt ttgctgatga atcaatacgc tggcgaagca
    21361 gctatgaggt ggaaaaacta cctgaagagc acacaaaaag ttcaactgca gaactttgcc
    21421 aactggccga aacacactct ctccacccta tttcactttc ccttctcgaa aagttgttaa
    21481 acagcacacg ggtcaataca cagccggata atcagcttgc ggcgttgtta tgcgcgcgta
    21541 taaatggcag tcctgcttgt ttttccacct gtatggattc atcaaatgaa tataaaaaaa
    21601 tcagcaccct tctgcgcaag ggcgaaaatg aaattaaccg atgggctgac cgtcattctg
    21661 ttgagcgcgc taccgttcag gcgatacaat ggctgacccg agcacccgat cgctttagcg
    21721 ccgcccagtt cagcccttta ctcgaacacg aaaaatcatc aacgcagatt attaatctgc
    21781 tggtatggag cgggctgtgt ggctggataa atcgcttaaa aatcgcgttg ggtgagacat
    21841 attaaccttg ccgcgtcaga cagattcgcg taaaactgtc agccgctcta atggccacca
    21901 aaatagacaa ttatgtttca ggacaacccg ctgctagcgc agcttaaaca gcaactgcat
    21961 tcccagacgc cacgcgctga aggggtggta aaagccacag aaaaaggctt tggcttcctg
    22021 gaagtcgacg cacaaaaaag ttatttcatt ccgccgccgc agatgaaaaa agtcatgcat
    22081 ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac gtgaatccgc agagccagaa
    22141 gaactggttg aaccgttcct gactcgtttc gtgggtaaag ttcagggcaa aaatgaccgt
    22201 ctgaccatcg ttcctgatca tccactttta aaagacgcca ttccttgccg cgcagcccgt
    22261 ggcccgaacc acgagtttaa agaaggcgac tgggcggttg ccgaaatgcg ccgtcatccg
    22321 ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat acatcacttt tggcgacgac
    22381 cactttgtac cgtggtgggt tacccttgcg cgccataatc tggaaaaaga agcaccagac
    22441 ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg aagatctgac ctcgctggat
    22501 tttgtcacca tcgacagtgc cagcactgaa gatatggatg acgccctttt cgctaaggcg
    22561 ctgccggatg acaaacttca gctgattgtg gcgattgccg atccaaccgc gtggattgct
    22621 gaaggcagca agctggacaa agccgcgaaa attcgcgcat tcaccaacta tctgcctggc
    22681 ttcaacatcc ctatgctgcc tcgcgagctt tctgacgatc tctgctcact gcgcgccaat
    22741 gaagtccgcc cggtactggc atgccgcatg acgctctccg ctgatggcac cattgaagat
    22801 aatatcgaat tctttgccgc caccatcgaa tccaaagcga agctggtgta tgaccaggtt
    22861 tctgactggc tggaaaatac cggtgactgg aagcctgaaa gtgaagcaat tgccgaacaa
    22921 gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt ggcgtcataa ccacgcacta
    22981 gtgtttaaag atcgcccgga ttaccgcttt attctcggtg aaaaaggtga agtgctggat
    23041 atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg aagaagcgat gattgccgct
    23101 aacatttgtg cggcccgcgt actgcgcgat aagctcggtt ttggcatcta taacgtgcat
    23161 atgggctttg atccggcgaa tgccgacgcg ctggcagcgt tgctgaaaac gcacggtctg
    23221 catgtcgatg ccgaagaagt actcacgctg gacggtttct gcaaactgcg tcgtgaactg
    23281 gatgcgcaac caactggttt cctcgacagc cgcattcgtc gcttccagtc atttgctgaa
    23341 attagcactg aacccggtcc tcactttggc ctcggtctgg aagcatacgc cacctggact
    23401 tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc tgctgaaagc ggttatcaaa
    23461 ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc aaatggccga acgtcgccgt
    23521 ctcaaccgga tggcagaacg tgatgttggc gactggttat acgcacgctt cctgaaagat
    23581 aaagccggga ccggcacccg tttcgcggcg gaaattgtcg atatcagccg tggcggcatg
    23641 cgtgtgcgtt tggttgataa cggcgctatc gcctttattc cggcaccttt cttacacgct
    23701 gtgcgcgatg aactggtttg cagccaggaa aacggcactg tacaaattaa aggtgaaacg
    23761 gtttacaaag taactgacgt tattgacgtc accattgccg aagtccgcat ggaaacccgc
    23821 agcattattg cgcgcccggt cgcgtaatct cctttcacgg cccattcctc atggatgggc
    23881 cgtttatttc cccgctctac cttcgttata tccaggcaga ttttttaact gactttcgtt
    23941 tgaaaactgg cgtttttcca aaactggatt acaaataaat atataaaaaa caatgcgttt
    24001 atccgccgtt aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgctg
    24061 ttaaccggag gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc
    24121 tcggatcgca caatccatac tggcggttga cggaaagcag cgatgctttg cgcttttcta
    24181 ccaccaaaac cacagaacct gatcgtacat tgcagttatc tgccgaacag gctgctcgca
    24241 tcagggaaat gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgaaagcg
    24301 atctttctga gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt
    24361 ctgcatggca tgacacaccg gcggttgctc gtgatttatc acacgggctt tcctttgctg
    24421 agcaggtagt ttctgaagca cattccgcaa tagtgattct cgacagccgg gggaatatcc
    24481 aacgcttcaa tcggttatgt gaagattaca cagggttgaa agaacacgac gtcattgggc
    24541 aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc
    24601 gtgtattttt tcgaagcggc aatgcatatg aagtcgaact gtggatacca acacgtaaag
    24661 gccagcggct gtttctgttt cgcaataaat ttgtccacag cggcagtggc aaaaacgaga
    24721 tttttttaat ctgttccggt accgacatta ccgaagagcg ccgcactcag gagcgactgc
    24781 gtattctggc aaataccgac agtatcaccg gactgccgaa tcgtaacgca atgcaggagt
    24841 taatcgatca cgctattaat caggcagata acaataaagt tggggttgtg tatcttgatt
    24901 tggataattt taaaaaggtc aacgacgcct atgggcattt gtttggtgac cagttattac
    24961 gcgacgtgtc attggctatt ttaagctgtc tcgaacatga ccaggtgttg gcgcgtccag
    25021 gtggcgatga gtttctggta ctggcatcca atacctcaca aagcgcgctg gaagcaatgg
    25081 catcacgaat tttgacccgc ttacgcctcc cctttcgtat tggtttaatt gaagtttata
    25141 ccagctgttc agtaggtatt tcgctctctc ccgaacatgg ttcggacagc gcgactatta
    25201 ttcgtcacgc cgacacagca atgtacacag cgaaggaagg cggacgagga caattttgcg
    25261 tttttactcc agaaatgaat cagcgggtat ttgaatatct ctggctggat accaacttgc
    25321 gtaaagcact ggaaaacgat cagttggtta tcgaaaatca cctagcgtgg cgaagtgcgc
    25381 agtctggaag cactagtacg ttggcagtca cctgaacgtg ggttgattcc accgttggac
    25441 ttcatttcct acgccgaaga gtcagggcta attgtgcctt taggccgttg ggtgattctc
    25501 gatgtcgtac gccaggtggc aaagtggcgg gataaaggca taaacctgcg agtggcggta
    25561 aatatttctg cacgtcagct cgccgatcaa accattttca ccgccctgaa acaggttctc
    25621 caggaactca attttgaata ctgccctata gatgttgaac tgacagagag ttgtctgatt
    25681 gagaatgatg aactggcact gtctgttatt caacaattta gccaactagg tgcgcaagtg
    25741 catctggatg attttggtac cggctactct tcactttcgc aactggcgcg ctttccgatc
    25801 gatgccatca aacttgacca ggtttttgtt cgagatattc acaaacaacc tgtctcgcag
    25861 tcactggtcc gggcgatcgt cgctgtggcc caggcattga atcttcaggt gatcgccgaa
    25921 ggtgtagaga gtgcaaagga agatgctttt ttaaccaaga acgggatcaa tgagcggcaa
    25981 ggatttttgt ttgccaaacc gatgcccgcc gtcgccttcg aacgctggta taaacgctat
    26041 ctgaagcgcg cataagtaga tgaatatata tacttagcgc tatatcccgt cattatatat
    26101 cgtattcctg gatgataatt agccgttgct gttaaacact tctcttttag cggcaatttt
    26161 tgcattattc attttccccc ttttgctata aggatgtcct tgatgtctga atttgatgcc
    26221 cagcgcgttg ccgaacgtat tgatattgtg ttagatatcc ttgtggcagg cgattaccac
    26281 tctgcgatcc ataatctcga gatacttaaa gcagaactct tgcgccaggt tgctgaatct
    26341 acgccagaca tcccgaagac accgtgggag atttaacaat cgtgccttca ggttttccta
    26401 tttgaggatg cccggcgcgt cagtattttt ttatttagta ttataacgtt ataagaatta
    26461 cagcgatgaa ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta
    26521 gcgtaaccga tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca
    26581 acaccctcga aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata
    26641 gtgatgacgt cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt
    26701 ttgccgcgtc actggttcaa ccgggcgaaa ccatctttat cgaaaatggc agcagcaatg
    26761 ccctgctcgc ccggactctg ggggagcaga agaaaaatgt cactatcatc acggtcagca
    26821 gctacatcgc gcatttgctg aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt
    26881 accagaaaaa aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac
    26941 atttcagcaa agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc
    27001 gcgatatgat gcgcaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag
    27061 tcctgactga cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc
    27121 gattcaatcg cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc
    27181 acagtaaact caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt
    27241 tgcagtttgg caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac
    27301 ctgttattaa cactctcaag atataaaatt attatcagcg atataacagg aagtaattat
    27361 cacgtgcgtg atataaccct gcgcgcgagc agatttcacg gaataatttc accaggctta
    27421 ttcttagcta ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt
    27481 attatgtttg taacgagcaa aaaaatgacc gcggctgttc tggcaattac tttggcaatg
    27541 tctctgagtg cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt
    27601 gcaggggcat taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggt
    27661 gcagctgtcg gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat
    27721 tatcgactcg caattataat acgctt
//
LOCUS       DADMFL010000050        27571 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.050, whole genome shotgun sequence.
ACCESSION   DADMFL010000050 DADMFL010000000
VERSION     DADMFL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.050"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            352..1650
                     /gene="kgtP"
                     /locus_tag="JNZ27_002100"
     CDS             352..1650
                     /gene="kgtP"
                     /locus_tag="JNZ27_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY8664432.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1647..1991)
                     /locus_tag="JNZ27_002101"
     CDS             complement(1647..1991)
                     /locus_tag="JNZ27_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY8664433.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(2016..3371)
                     /gene="pssA"
                     /locus_tag="JNZ27_002102"
     CDS             complement(2016..3371)
                     /gene="pssA"
                     /locus_tag="JNZ27_002102"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY8664434.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3485..6145)
                     /gene="pat"
                     /locus_tag="JNZ27_002103"
     CDS             complement(3485..6145)
                     /gene="pat"
                     /locus_tag="JNZ27_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY8664435.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6177..6875)
                     /locus_tag="JNZ27_002104"
     CDS             complement(6177..6875)
                     /locus_tag="JNZ27_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY8664436.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6944..7363)
                     /gene="trxC"
                     /locus_tag="JNZ27_002105"
     CDS             complement(6944..7363)
                     /gene="trxC"
                     /locus_tag="JNZ27_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY8664437.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7570..8607
                     /locus_tag="JNZ27_002106"
     CDS             7570..8607
                     /locus_tag="JNZ27_002106"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8664438.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8655..9344)
                     /gene="ung"
                     /locus_tag="JNZ27_002107"
     CDS             complement(8655..9344)
                     /gene="ung"
                     /locus_tag="JNZ27_002107"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY8664439.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9649..10032
                     /gene="grcA"
                     /locus_tag="JNZ27_002108"
     CDS             9649..10032
                     /gene="grcA"
                     /locus_tag="JNZ27_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY8664440.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10088..10675)
                     /gene="eamB"
                     /locus_tag="JNZ27_002109"
     CDS             complement(10088..10675)
                     /gene="eamB"
                     /locus_tag="JNZ27_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY8664441.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGSVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10779..11660
                     /locus_tag="JNZ27_002110"
     CDS             10779..11660
                     /locus_tag="JNZ27_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8664442.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11695..13029)
                     /gene="srmB"
                     /locus_tag="JNZ27_002111"
     CDS             complement(11695..13029)
                     /gene="srmB"
                     /locus_tag="JNZ27_002111"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY8664443.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13161..13898
                     /gene="trmN"
                     /locus_tag="JNZ27_002112"
     CDS             13161..13898
                     /gene="trmN"
                     /locus_tag="JNZ27_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY8664444.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13883..15505)
                     /gene="nadB"
                     /locus_tag="JNZ27_002113"
     CDS             complement(13883..15505)
                     /gene="nadB"
                     /locus_tag="JNZ27_002113"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY8664445.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15761..15916
                     /gene="rseD"
                     /locus_tag="JNZ27_002114"
     CDS             15761..15916
                     /gene="rseD"
                     /locus_tag="JNZ27_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY8664446.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15913..16488
                     /gene="rpoE"
                     /locus_tag="JNZ27_002115"
     CDS             15913..16488
                     /gene="rpoE"
                     /locus_tag="JNZ27_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY8664447.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16521..17171
                     /gene="rseA"
                     /locus_tag="JNZ27_002116"
     CDS             16521..17171
                     /gene="rseA"
                     /locus_tag="JNZ27_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY8664448.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17171..18127
                     /gene="rseB"
                     /locus_tag="JNZ27_002117"
     CDS             17171..18127
                     /gene="rseB"
                     /locus_tag="JNZ27_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY8664449.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFREVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18124..18603
                     /gene="rseC"
                     /locus_tag="JNZ27_002118"
     CDS             18124..18603
                     /gene="rseC"
                     /locus_tag="JNZ27_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY8664450.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18801..20600
                     /gene="lepA"
                     /locus_tag="JNZ27_002119"
     CDS             18801..20600
                     /gene="lepA"
                     /locus_tag="JNZ27_002119"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY8664451.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20616..21590
                     /gene="lepB"
                     /locus_tag="JNZ27_002120"
     CDS             20616..21590
                     /gene="lepB"
                     /locus_tag="JNZ27_002120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY8664452.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21862..22542
                     /gene="rnc"
                     /locus_tag="JNZ27_002121"
     CDS             21862..22542
                     /gene="rnc"
                     /locus_tag="JNZ27_002121"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY8664453.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22539..23444
                     /gene="era"
                     /locus_tag="JNZ27_002122"
     CDS             22539..23444
                     /gene="era"
                     /locus_tag="JNZ27_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY8664454.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23456..24184
                     /gene="recO"
                     /locus_tag="JNZ27_002123"
     CDS             23456..24184
                     /gene="recO"
                     /locus_tag="JNZ27_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY8664455.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGIGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24196..24927
                     /gene="pdxJ"
                     /locus_tag="JNZ27_002124"
     CDS             24196..24927
                     /gene="pdxJ"
                     /locus_tag="JNZ27_002124"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY8664456.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24927..25307
                     /locus_tag="JNZ27_002125"
     CDS             24927..25307
                     /locus_tag="JNZ27_002125"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY8664457.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25727..25807
                     /gene="shoB"
                     /locus_tag="JNZ27_002126"
     CDS             25727..25807
                     /gene="shoB"
                     /locus_tag="JNZ27_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY8664458.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26001..26261)
                     /gene="yfhL"
                     /locus_tag="JNZ27_002127"
     CDS             complement(26001..26261)
                     /gene="yfhL"
                     /locus_tag="JNZ27_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY8664459.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26476..>27571
                     /locus_tag="JNZ27_002128"
     CDS             26476..>27571
                     /locus_tag="JNZ27_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY8664460.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTRQAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6804 a         6867 c         7229 g         6671 t
ORIGIN      
        1 catgcgagag tagggaactg ccaggcatca aactaagcga aaggccatcc gtcaggatgg
       61 cctttttgca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca
      121 catatgacag attcagcaac ggatgcggtt tccccaactt gcctacttcc atacgtgtcc
      181 tccttaccag aaatttatcc ttaagctcct caataaccat tttcctgcta actaaattca
      241 tggtttaggt tgcataatga tacgcaacaa atgtataaca ttttatttac caaaagaaat
      301 aaacaaaagc gaccaacaaa agcatcggat tacggcagga gacataatgg catggctgaa
      361 agtactgtaa cggcagacag caaactgaca agtagtgata ctcgtcgccg catttgggcg
      421 attgtggggg cctcttcagg taatctggtc gagtggttcg atttctatgt ctactcgttc
      481 tgttcactct acttcgccca cattttcttc ccttccggga acacgacgac tcaactacta
      541 caaacagcag gtgtttttgc tgcgggattc ctgatgcgcc caataggcgg ttggctattt
      601 ggccgcatag ccgataaaca tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg
      661 tgtttcggat cgctggttat cgcctgcctc ccaggttatg aaactatagg tacgtgggct
      721 ccggcattat tgcttctcgc tcgtttattt cagggattat ccgttggcgg agaatatggc
      781 accagcgcca cctatatgag tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca
      841 tttcagtatg tgacgttgat cggcggacaa ctgctagccc tactggttgt cgtggtttta
      901 caacacacca tggaagacgc tgcactcaga gagtggggat ggcgtattcc tttcgcgtta
      961 ggagctgtgt tagctgttgt ggcgttgtgg ttacgtcgtc aattagatga aacttcgcaa
     1021 caagaaacgc gcgctttaaa agaagctgga tctctgaaag gattatggcg caatcgccgt
     1081 gcattcatca tggttctcgg ttttaccgct gcgggctccc tttgtttcta taccttcacc
     1141 acttatatgc agaagtatct ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc
     1201 attatgactg ccgcattgtt tgtattcatg cttattcaac cactcattgg cgcgctgtcg
     1261 gataagattg gtcgccgtac ctcaatgtta tgtttcggtt cgctggcagc catttttacc
     1321 gttcctattc tctcagcatt gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg
     1381 atgtgtgccc tgctgatagt gagtttttat acatcaatca gtggaatact gaaggctgag
     1441 atgttcccgg cacaggttcg cgcattaggc gttggtctgt catatgcggt cgctaatgct
     1501 atatttggtg gttcggcgga gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca
     1561 gccttcttct ggtatgtgac cttgatggcc gtggtggcgt ttctggtttc tttgatgcta
     1621 catcgcaaag ggaaggggat gcgtctttag tgacgggtca gttgccagac ggtatagccg
     1681 gtgcttgcac cggcgacatc ccaggccaaa tccttccagc tccagccgct cccttcgggg
     1741 cggctatccc aaagctcttt tgacgccccc aaactgacag agaacatcaa tccaaacatg
     1801 gcactgcgat cccggctcat cccctgatgc tgtgaatatt catttccggc ggcggaaagt
     1861 atcgccgagg cgataaagtg ttgagcttta tcctgcccgc tccagctatc gttagccata
     1921 tggctgcatc cagaaagtaa caatagcgaa cagacaaaaa gaatacgcaa aacgtggcct
     1981 ccaaaaaaca aaccccgtac aggacggggt tgtgattaca ggatgcggct aattaatcgg
     2041 tcgatgcgga tacggcgcaa acggcggatg agtttacgaa ccttcaccgg ataatcggca
     2101 atactttgca gatcgcgata gtgcttaacg atggtggtac gctcgcggat cagctccagt
     2161 tctttctctc gctgcggcgc aagctccagt tgtggatcgt ggatcaaaat ggcgttttcc
     2221 agatccagac gccaggcgcg cgggttcagg ttattaccgg tgatcaacat ccacttatca
     2281 tcaacccaca tccctttcag gtgataggtg ttgtcgtcat ctttccataa ccgaaccact
     2341 agctggtcag tattgacgta atactgcaaa cggctcagga aacgacgcag attgatctca
     2401 tagagataag gcaatgcgcc aattatcttg aaaggttcat cttccggaat gtagaagtcg
     2461 ttcgcggttt tatcaccaac aataatttcg acctttttcc cttcgcgcag caactggata
     2521 atattgcgca caaggattgc tggcaggttg aagtatggcg tacagatggt tagtttctgc
     2581 tcggcacaag gcataagatg gaaaatggtc ttgttcaaca gactcgattt ccccagcccc
     2641 actagcggcg ttacggaaag ctgatcgttg tcggcatcgc cctggaaatg ataagcggca
     2701 tcacgcagct cctggcggaa cagacgaata tcgttcttga tttccgggct ttttggccga
     2761 ttaacatcat ccagacgatt aacgccgcgg ccattcataa tattctgtgt aacccattca
     2821 aacataatgt ctgacatctt acggttacgg atcagatgat aacggtcgta gcgatattta
     2881 tcgtgttgat gcaggtaaac atcgttcagg ctggcaccgc tataaagtac gctatcgtcg
     2941 atgataaagc ctttaaagtg cagaacacca agagcttcac gggtattgat tggaacgcca
     3001 taaaccggaa catctacgcc cggattttcc tgcgccatgc ggcagtacca gtcagcgtta
     3061 gtgttagatg ccgcagcgcc aatgcgtcca cgttgtgcac gatgccagtc gaccagcacc
     3121 cgcacatcca gttccggacg ctgcctttta gcctcataca acgcgttcag aatgcctttg
     3181 ccaccgtcat cctgttcgag atacagggcg acaatgcaaa tgcgctgctt cgcgctggct
     3241 attttttcca gcagcgtctc ccggaagtcg gcgggagcgt aaaagaaatc gacatcatca
     3301 actgattgag aaatcttggg tagttgggca aggtgttgtt gatgtttatt acgcttaaat
     3361 tttgacaaca tcacagtgca tttcttctct gttcattgaa gggtcctctg tgcaatgcag
     3421 acgacataag cgggcaataa taacaccagt cccgattaag tggtcaacat ttccagtacc
     3481 ttactcatga ttcctcgcgc cgggcaagat ttagcgtaag cccaacgatc ccctcttcga
     3541 gctggatatc aacgttaaac ccgagcttgc gggctagcgc caccatgcca cgattgtttg
     3601 gcatcgtaat accattcaga cgttgtagtc cgtgatttcg cgtataggta atcaactttt
     3661 ccattaagcg tcgacctaag cctaaccctt tgagatccga gcgaaccagt acagcaaatt
     3721 cggcatcgat gttatcagga tcggaaatcg cacgcgtgac gccgaggatc tcttccgttt
     3781 gatcaatacg tcgtaccgct acaaacgcca tttcccgatc gtagtcgatc tgtgtcatgt
     3841 tggctaaatc ttcatgggta aattcgttga tctcgctaaa gtagcggtaa taaagatctt
     3901 ctttggtgac tcgcgaaatg aattgctgaa gttgtggctc atcttctggc aaaatcgggc
     3961 ggaacaagca gcgttcaccg tttttcaatt ctacccattc ttccagctga tgcggataag
     4021 ggcgcactgc cagccgactc tcgttatcgc cttcaaacgg cgagatatcc agcgtgacat
     4081 ccagcgcggt aaattcactg ccagaagcca gcaaaggatg aatatccaga cgctgaattt
     4141 ccgggcaatc gacaatcaag ttggaaacct gcaccagaag ctggctcaag cctgcaacat
     4201 ccaatgggcg tagcgcactg cgcgcacgaa tctttttact tttgatcccc tgaataacca
     4261 gatagcgggc caggttcatg ttcagcggcg gcagtgcgac gacggcttga tcttcaggac
     4321 gccactccac accgccttca cccagcatga tcaacggccc gaaaaccgga tcgtgctcaa
     4381 ccacaacccg caactcctga gcgccagcac ggttagccat actttgcacc aacaggccgt
     4441 ggacccgcgc ctgtggccag gccattttta cgcgatcgaa aatagcgttc gccgcttgct
     4501 ggacttcatt ggctgtacgc aggtaaagca tgacgccctg aacttccgat ttatgtggaa
     4561 tatccggcga acgcaatttc agcgccaccg gataaccaat ctgttcggca atatgcaccg
     4621 cttcggtgct atcgctggca atccaggttg ggagcgtgtt catgccatac gcttgcagga
     4681 tgggctgaac ttcatgggta tcgagcgacg tagccccttc ggcaatcgcc tgttgcaaca
     4741 gaagatgcgc ttctgcggta ttggaagtca gattgctggg caacgccggc gtttcgcgta
     4801 gttgcttctg attacgccgg tactccacca tatgcataaa agcagtgatg gttccttccg
     4861 gggtacggta ggtcggcagc ccggcttcgc tgaataaacg tcgtgcctct tgcgaggagt
     4921 gctcgccgca ccagttcgtc agcaaagaga catatttgct gcggggatga tgctttaccg
     4981 cttcaattaa tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc gaatgaataa
     5041 ccatcagcgc atcaaaatcc tggctgtgga gcagaatgtc cagcgttttg acataatgct
     5101 cgctgctggc gtcatcgcgc agatcgagcg ggttagagac tgccacatgt tctggcaatg
     5161 catcgcgcaa tttctggcag gtttcttcgc ttagcgttgc cagcttgcca ttgcgtgacc
     5221 ataaggcatc cagcgccagc gcggcaggcg cagcaccgtt gctgataatc atcaaccggt
     5281 cgccacgcag cgggcgcata tggctaaggg tttccaccgc cgaaaacagt tcgtgggtat
     5341 cctgtacccg caacaaaccg gcacgctgaa tagccgcatc ccatgccggg tccattcctg
     5401 ccgtggtgtt gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc accagaatcg
     5461 gtttattacg cgaggcacta cgggccgccg aaacaaagcg tcgcgcgtcg cttaactgtt
     5521 cgagatagag caggatggcg ctggttttac tgtcgcgcgc cagatagtcg agcaattcat
     5581 caacgtcgat atccaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct
     5641 gttgcgccca gtcgaggatg gtgttggaga cggcagccga ttgcgaaata aacgccagct
     5701 tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta
     5761 aacccagact gtttggtcca agcaggcgca tgttatggcg cagggcgcag gcgcggagat
     5821 cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga
     5881 gctcttccag aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg
     5941 taaagggcaa gctggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg
     6001 gcgtcaccgg gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt
     6061 aacctgcgcg attgggtttc atcgacgcgc caattaccgc tatcgatttt ggtcgcagta
     6121 gtgcttccag tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa
     6181 acgctttcta actgttctgc tgtgatgcta cccagatgtt gcgtttttcc tgccagatag
     6241 cgtgttttaa agcgggtaaa atgctcgcct aaccctgctg ccaccccggt atcgccggcc
     6301 atatctaaca gtgcgatggc tacctcggca gtacaatatt ggcctgcagc ctgggcttca
     6361 cgcaggcgat aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga
     6421 tacggacttt tacgaaacat cttgcgagct tccggccagg taccatcgag catgataaac
     6481 agcggtggct taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag
     6541 gcgggaaaga ccaccattgg ctgataatcc gggttttgta ccagatccag caaatcctgc
     6601 gagggttcgg tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga
     6661 cgcccggtat tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt
     6721 tttgcttgtg ctggggtaat tgtcgaacag agacataatt tctctggcaa aagacagcgt
     6781 tggcagcgac gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc
     6841 tcggcgcgta actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt
     6901 gtcgcagagg agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca
     6961 gccagctatc gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt
     7021 ttttgaaaat catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt
     7081 cagcttcggt attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt
     7141 caaaaattgg tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca
     7201 caggtagatc atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc
     7261 cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg
     7321 gaatgcgatt gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga
     7381 gtaaggagac ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg
     7441 acattgttcc agacaacaat ctgttttatt caataggtat gatagtcgca ggctttggta
     7501 cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg
     7561 gagaaaagca tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc
     7621 cgcagtgatg atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg
     7681 cgtccaggaa aatctcgtgc tgatggtggc cgtcgccccg cccgcgatga caaacagagt
     7741 cagccccgtg accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt
     7801 gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg
     7861 gaagtgttgc gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag
     7921 gcactgttcc agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg
     7981 ccgcgtttta aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg
     8041 gatgaagcgg aattgacaaa agcgtcaggc acggaacatc acggcggcgt ttgcttcctg
     8101 atcaaaaagc gtaacggtac aaccgtgcag cagtgggtaa tccaggcagg cgcgcaggat
     8161 tgcgttctgg cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc
     8221 tgcgcgcact tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg
     8281 gcggctatcc gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac
     8341 attgttaacg tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc
     8401 gagcagggta aaccgctgtt caaaaccagt ctgccagcga aaatggtact ggtgctgggt
     8461 caggaatatg aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt
     8521 gacggtacgg gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt
     8581 gaatggtggc gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc
     8641 atttccccgc aaatttactc actctctgcc ggtaatactg gcatccagtc aatcggcgtc
     8701 tcgccacgtt gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca
     8761 cgatgcgccg aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct
     8821 ataatcgccc ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg
     8881 tgctggttaa tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg
     8941 gaatgcgcct gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc
     9001 gcccagcttt cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc
     9061 tctttataca tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc
     9121 agaccatgcg cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg
     9181 tcacccaact cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg
     9241 acgccggact gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc
     9301 ttctcttcag ccagcacgtc atgccaggtt aattcgttag ccatcttgct ctcctgcgaa
     9361 tcttcaatcc gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta
     9421 gacttaaacg agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg
     9481 taaaacaata aaatccattt acgaccacaa ttgatgtgtg gtttttgttg atttaaatca
     9541 aagaatcaag ggtggttgag gagtatatat acactcaagc aacaatggtt ttaccaattg
     9601 gccgcgacag gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt
     9661 atccagatta ctaaagccgc taacgacgat ctgctgaact ctttctggct gctggacagc
     9721 gaaaaaggcg aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt
     9781 gcagtaagca aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa
     9841 gttcgcgttg aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa
     9901 gatgcagtta agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca
     9961 gttcgcttta actctctgac tccggaacag cagcgcgacg ttatcgctcg tacctttact
    10021 gaaagcctgt aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt
    10081 tttttcgtta atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat
    10141 ttaactggcg accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc
    10201 acacactgcc aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta
    10261 gcgcctgtgt ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt
    10321 tgacgttcac aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc
    10381 cgtcttcctt tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc
    10441 ccgcccaact caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc
    10501 ccgcacacag taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac
    10561 tttgacgaaa tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg
    10621 tcatagcggt aatcagggtg taagtccaaa aagcacttaa aaggatcggt gtcacttgtt
    10681 atctcctctg ttttggtaga cagtacccga cacggaacat ttaaaaaaaa tgaatgtttt
    10741 tatattctaa attcgatatt ttcgataggt ttggggttat ggatctgcgc cgttttatta
    10801 cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc ttcgcaaaaa ttgtgctgta
    10861 cacaatcgac ggtgactttt catattcagc agcttgagca ggagttttca gtccagttat
    10921 ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg aaaaaagttg ctgccgcaca
    10981 tttatgaact tacccgagtg atggatacgc ttcgtgaggc tgccaaaaag gagtcagatc
    11041 cggacgggga actacgcgtt gtttcgggcg aaacgttgct ctcttaccga atgccgcagg
    11101 tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt atcgttgcag gcgctgaact
    11161 gttacgtgat ccgtgatgcg ctactgaatg atgaggccga tgtcggcgtc ttttatcgcg
    11221 tagggaatga tgatgccctg aaccgacgag agttgggtga acaatcgctg gtgctggtgg
    11281 cttcaccaca aattgcagat gtcgatttta ctgagccggg aagacataac gcctgtagct
    11341 ttattatcaa cgaaccgcaa tgtgtcttcc ggcagatatt tgagagcacg ctgcgacagc
    11401 ggcggatcac ggtggaaaac accattgagc ttataagtat cgaaagcatc aagcgttgtg
    11461 tcgcggcgaa tatcggtgtc agttacttgc cccactttgc ggtggaaaaa gagcttgaga
    11521 gcggagaatt aattgaattg ccttttggcg aacagtcgca gaccattacg gcaatgtgcg
    11581 cacatcatgc tggaaaagcg gttagcccgg cgatgcacat ttttattcag tgtgttgaag
    11641 agtgttttgt ggttgcgtaa aaaaacaatg ccgggcttaa acccggcatt gagattactc
    11701 ttctgtcgtt tgcggtggca cgcccgttcc gctcggttta cggcgcttac caatattttt
    11761 ggtgtcgcga tggcgttttt tcacccgcgg cttctctttc tctttagctt ttttcttctc
    11821 ggcgcgtttt gccagtactt tcttcgatgg cttgccggtc tgcttctcgc ttggcgcacg
    11881 cgttttcggg cgtaactcat caataacgcg agctttaatc ggctcttcaa tgtaacggcc
    11941 aaccttgccc agcagcaaat ggtcatgggc ttccaccagc gaaattgcgg tgcctttacg
    12001 accggcgcgc gcggtacgtc cgatacgatg caaataagta tcgccactgc gcggcatatc
    12061 gaagttaaag acgtggctga cgtcaggaat gtcgataccg cgcgcggcaa catcggtggc
    12121 aaccagtacg tttacgcggc cttcggtcag gcgtttaatt gcttcgttac gcttgccttg
    12181 taccatctca ccttcgagat agcagttgtt gatgcccgct tcgcgcagcc agtttgccag
    12241 ctcatgcaca cgttcacgct tacgcacaaa cacgattgag cgagtcgctt caggctgttt
    12301 taacaggtgc accagcaatg cggttttatg ttccaggtca tcggcgcggt aataccactg
    12361 atgaattttt ttgcgctcac gggtggacgg attggcagaa acttccaccg gatcttccag
    12421 cagacgctcg gcaaagtcct gaatcgcatc gccttccagc gttgccgaaa agagcagggt
    12481 ctgtttacgc cagcgagttt cgccagcaat atgttcgata tcctgagcga agcccatatc
    12541 cagcatacgg tctgcttcgt cgaggatcag cgtttcaacc gcgcggcaat cgaagttctc
    12601 ttcttttatg tattgcagca gacgtccggt cgtggcgacc acgatgtcct gattttcgct
    12661 gaacacttcc gcgtggttca tataggctac gccgccggtg atggtggcga tatccagatg
    12721 cgtatgtttc gccagttcgc gggcatgatc ggccacctgc atcgccagct cgcgagttgg
    12781 ggtgaggatc aaaatacgcg gcggaccgga tttcttacgt gggaaatcga gcaggtgctg
    12841 caacgctggc agcagatacg ccgccgtttt accggtgcct gtcggcgcag aaccgagtac
    12901 atcacggcca tcgagcgcag gcggaatggc ggcagcctga atggcggtcg ggcgagtgaa
    12961 acctttatcc tggagggctt ccagcaggct ttcgtcgagt tcaagttcgg aaaaagtcgt
    13021 tacagtcatg ttctacctct gtgtggggcg ctgattatag acgttacggc tgcaatcttc
    13081 atctgtttgt atggatatcg cttctcgggt attgttctca cccggtgcta tcgccgtttt
    13141 tcctgcaagg ttttattttc atgtcacagt ctacatccgt gcttcgtcgt aatggattta
    13201 cttttaaaca gttttttgtt gctcacgatc gctgtgcgat gaaagtggga acggatggca
    13261 ttttgctggg cgcatgggca ccggtggctg gggtgaaacg ttgccttgat atcggcgcgg
    13321 gtagcgggtt gctggcactg atgctggcgc agcgaaccag tgacagcgtg ataattgatg
    13381 ccgttgagct ggaaagtgaa gctgccgcgc aggcgcagga aaatatcaac cagtccccgt
    13441 gggcagagcg gattaacgtc catacggcgg atattcagca gtggatcaca cagcagacag
    13501 tacgcttcga tttaatcatc agtaacccac cttactatca gcagggagta gagtgcgcga
    13561 cacctcaacg ggaacaggct cgctatacca ctacgcttga tcacccatcg ctgctgacct
    13621 gtgcggcgga gtgcattacc gaagagggat ttttctgcgt ggtgctgcca gagcagattg
    13681 gtaatggttt tactgaactg gcattaagca tgggctggca tttacgtttg cggacggatg
    13741 tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct ggcattctcc ccgcaggcgg
    13801 gagaatgctt tagcgatcgc ttagtcattc gtgggccaga ccagaactat tccgaagcgt
    13861 atacggcgct gacccaggct ttttatctgt ttatgtaatg attgccgggg gaaaggatcg
    13921 atggaccgga atgcgtgagc agttccgggt aatccagcgt aaaatgcagc ccccggcttt
    13981 ctttgcgcat catcgcgcag cgaacaatca actcggcaac ctgtaccaga ttacgcagct
    14041 ccagcaaatt atttgagacg cggaaatggg cgtaatattc gtctatttct tgttggagca
    14101 tggttatccg ccgcagggcg cgttccaggc gcttcgttgt gcgcacaatg ccaacgtaat
    14161 cccacataaa cagccgtagc tcgtgccagt tatgctgaat tactacccgt tcgtcagggt
    14221 tctcaacgcg gctttcatcc cacggcggta acgtactgac gccgtgggca taaggcatac
    14281 gtctggtgat atcttctgcc gccgaccagc catagaccag acactccagc aatgaattcg
    14341 aggccatgcg gttagcgccg tgtaagccgg tataactcac ctcgccaatg gcatacaagc
    14401 cctcgacgtc cgtacgccca tgatcatcaa ccattacacc accgcaggta tagtgtgcag
    14461 caggcacaat cggtaccggt tcttgtgtga gatcaatccc cagcccgagc aatttttcat
    14521 aaatcatcgg gaaatgctgg cgaataaaat cggcgggctt atggctgatg tcgaggaaca
    14581 tacaatctgc gccgaggcgt ttcatttcat ggtcaatggc gcgggcgaca atatcgcgcg
    14641 gggccagttc gccgcgctca tcaaaatcgg gcataaaacg cgtgccatcc gggcgcttga
    14701 gataagcgcc ttcgccgcgc agtgcttccg ttaacaggaa attgcgtgcc tgtgggtgat
    14761 acagtgcggt agggtggaac tgattaaatt cgagattggc aacccgacag cccgcgcgcc
    14821 acgccatagc aatgccatcg ccagaagaaa tatccggatt ggtggtgtac tgatacacct
    14881 tcgacgcacc gccggttgcc agtaccactg cttttgcgtg gcaggtttcc accgtttctt
    14941 tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt gcccggcagg ccaattttgt
    15001 cagagacaat cagatcaact gcgttgctgc gctccagcac acgaatattc ggatggttct
    15061 gcgccttgct caccagcgtg gattgtactt ctctaccggt ggcgtcggcg gcatgaagaa
    15121 tacgacggtg actatgtcca ccttcacggg tcagatggta gctttcttcg ccattcggtt
    15181 gaacgtgggt atcaaacaaa accccctggt caattaacca ttgcacacag gaacgagcat
    15241 tgctggcgac aaattcaact gcatggcgat cgcaaatacc agccccggaa atcaatgtgt
    15301 cttccacatg cgagtcaatg ctgtcagttt catcaaacac ggcggcaata ccgccctggg
    15361 cataaaatgt tgaaccttcc gttaccgggc ctttacttag aacgatgacc tgatgctggt
    15421 cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct accaataatc aacacgtcac
    15481 atgaatgttc agggagagta tttattttct ttgtttaatt tactaaacat tgtttggtca
    15541 gcatagcatc atgttgtgcg aataaacacc tgctatttta atatttgtta cagtggctaa
    15601 acacgttgac gcagggcggc gagaaaaaga gaagttactg gctggtggag gattaggtgg
    15661 tgaaataaaa aggccgttgg gttactcttc aggcagttaa atgggcattt ctacacagat
    15721 aatgcgatgt tcagattctg tagacttata atgatagata atgctccgtc tacagcatga
    15781 caaacaaaaa cagatgcgtt acggaacttt acaaaaacga gacactctaa ccctttgctt
    15841 gctcaaattg cagctaatgg agtggcgttt cgatagcgcg tggaaatttg gtttggggag
    15901 actttacctc ggatgagcga gcagttaacg gaccaggtcc tggttgaacg ggtccagaag
    15961 ggagatcaga aagcctttaa cttactggta gtgcgctatc agcataaagt ggcgagtctg
    16021 gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg tacaagaagc ttttattaaa
    16081 gcctatcgtg cgctggattc gttccgggga gatagcgctt tttatacatg gctgtatcgg
    16141 attgctgtaa atacagcgaa aaattacctg gttgctcagg ggcgtcgtcc accttccagt
    16201 gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg gcgcgttgaa agaaatttcg
    16261 aaccctgaga acttaatgtt gtcagaagaa ctgagacaga tagttttccg aactattgag
    16321 ttcctcccgg aagatttgcg catggcaata accttgcggg agctggatgg cctgagctat
    16381 gaagagatag ccgctatcat ggattgtccg gtaggtacgg tgcgttcacg tatcttccga
    16441 gcgagggaag ctattgataa caaagttcaa ccgcttatca ggcgttgacg atagcgggat
    16501 actggataag ggtattaggc atgcagaaag aacaactttc cgctttaatg gatggcgaaa
    16561 cgctggatag tgagctgctt aacgaactgg ctcataaccc agaaatgcag aaaacctggg
    16621 aaagctatca cttaatccgt gattcaatgc ggggtgatac tcccgaggtg ctccatttcg
    16681 atatctcttc acgcgtgatg gccgccattg aagaagagcc agtacgtcaa tcggcgacat
    16741 tgatcccgga agcccagcct gcgccgcatc aatggcagaa aatgccattc tggcagaaag
    16801 tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc cgcatgcgta tcgcttgcag
    16861 ttatcgttgg cgtccagcac tataatggac aatctgaaac gtcccagcag cccgaaacgc
    16921 cggtatttaa tacactgccg atgatgggta aagccagccc ggtaagcctg ggagtacctt
    16981 ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca ggagcagcgt cgtcgcatta
    17041 atgcaatgtt gcaggattac gaactgcaac gccgactcca ctctgaacag cttcagtttg
    17101 agcaggcgca aacccagcaa gccgctgtac aggtgccagg aattcaaact ttaggaacgc
    17161 aatcgcagta atgaagcaac tttggtttgc catgtcatta gtgacaggta gcctgttatt
    17221 ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta ttacagcaga tgaacctggc
    17281 cagtcagtca ctgaattacg agctgtcatt catcagcatc aataaacagg gtgttgagtc
    17341 tctgcgttat cgacatgcac gcctcgataa ccgtcctctt gcacaattgt tgcaaatgga
    17401 tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc agctattttg aaccgggact
    17461 tgaaccgttc acgcttaatg gcgattacat tgttgattct ctgccatcgc ttatctatac
    17521 cgatttcaaa cgcctttctc cttactacga ctttatctcc gtcgggcgca cgcgtattgc
    17581 tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat ggtacacgct acagctacat
    17641 cgtgtggatg gacaccgaat cgaaattacc gatgcgggtt gatctccttg atcgcgatgg
    17701 tgaaacgctg gaacaatttc gcgtgattgc ttttaacgtc aatcaggata tcagcagcag
    17761 tatgcagacg ctggcgaagg caaatttgcc gccgttgctt tctgttcctg taggtgaaaa
    17821 agctaaattc agctggacgc caacctggtt gccacagggt tttagagaag tttccagtag
    17881 tcgacgtccg ctaccgacga tggacaacat gcctatcgaa tcacgtctct attccgacgg
    17941 attattcagc ttctcggtaa acgttaaccg cgctacgcca tcgagcaccg atcagatgtt
    18001 gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat aacgccgaaa tcaccattgt
    18061 cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag aatattaagt tcggggcagc
    18121 gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa acgggcaggc gctggtcagt
    18181 tgtgatgtta aagcttcatg cagcagctgc gcttcacgcg ccggttgcgg tagccgcgtg
    18241 ttgaataaac ttggcccgca aaccacgcat accattgtcg taccctgtga tgagccgtta
    18301 gtgccggggc aaaaagtgga attggggatc gccgaaggca gcctgcttag ctccgcatta
    18361 ctggtttata tgtcgccgct ggtgggatta ttcctcatcg cttcgctatt tcagctactc
    18421 tttgcttctg acgtcgcagc attatgtggt gcggtcctcg gcggtattgg cgggttcctg
    18481 attgcgcgcg gctactcgcg taagtttgct gcccgggcag aatggcaacc gatcatttta
    18541 agcgtggcat tgccgcctgg ccttgtgcga tttgaaacat cttcggaaga cgcgagccag
    18601 tgatttcact ggctttgttg cattttgcat aacaaatgcg aggacgtttc cagataccac
    18661 aatccatacg gtatgaacat ttcctcgcct caatgttgta gtgtagaatg cggcgtttct
    18721 attaatacag acgttaagct cagaacagcg acttctaaaa gcctggttaa ccgggcatta
    18781 aggcacaata atcatacttt atgaagaata tacgtaactt ttcgatcata gctcacattg
    18841 accacggtaa atcgacgctg tctgaccgta ttatccagat ctgcggtggc ctgtctgacc
    18901 gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga gcgtgagcgt ggcattacca
    18961 tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc tatcagctta
    19021 actttatcga caccccgggc cacgtagact tctcctatga agtttcccgt tcgctggctg
    19081 cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtagaagcg caaaccctgg
    19141 caaactgcta caccgccatg gaaatggatc tcgaagtggt gccggtactg aacaagattg
    19201 acctgccggc agccgatcct gaacgcgtgg cggaagaaat tgaagatatc gtcggcatcg
    19261 acgccaccga tgcagtgcgc tgttcagcga aaaccggcgt tggcgtgcag gacgttctcg
    19321 aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc ggaaggcccg ttgcaggcac
    19381 taattatcga ctcctggttc gacaactacc tgggcgttgt ttcacttatc cgtattaaaa
    19441 acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag acctataacg
    19501 ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg aaatgtggcg
    19561 aagtaggctg gctcgtatgt gcgattaaag atatccacgg cgctccagtc ggcgatacct
    19621 taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg ctttaagaaa gtcaaaccgc
    19681 aggtatacgc cggtctgttc ccggtaagtt ccgacgacta tgaagccttc cgtgacgcgc
    19741 tgggtaaact cagcctgaac gatgcctcac tgttctatga gccggaaagc tccagcgcgc
    19801 tgggctttgg tttccgctgc ggcttccttg gcctgctgca catggagatc atccaggagc
    19861 gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta gtgtatgaag
    19921 ttgaaaccac gtcaagggaa gttatctacg tcgacagccc atccaagctg cctgcggtaa
    19981 ataacattta cgaactgcgc gagccgattg cagagtgtca catgctgctg ccgcaggcat
    20041 atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg cgtgcagacc aatatggttt
    20101 accacggtaa tcaggtggcg ctgacgtacg agatcccgat ggcggaagtg gtgctcgact
    20161 tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc tctggattac aacttcaaac
    20221 gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa cgtgttgatg
    20281 cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag ttggtggaga
    20341 agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca gcgattggta
    20401 cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa aaacgtactg gctaaatgtt
    20461 atggcggcga tatcagccgt aagaaaaagc tgctgcagaa gcagaaagaa ggtaagaaac
    20521 gcatgaagca gatcggtaac gttgagctgc cgcaggaagc gttcctcgcc attctgcacg
    20581 tcggcaaaga caacaaataa cccttaggag ttggcatggc gaatatgttt gccctgattc
    20641 tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc tttttcgcac
    20701 ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc cggtgactca ctggataaag
    20761 caacgttgaa aaaggttgca ccgaagcctg gctggctgga aaccggagct tctgtttttc
    20821 cggtgctggc tatcgtattg attgtacgtt cgtttattta tgaaccgttc cagatcccgt
    20881 caggttcgat gatgccgact ctgttaatcg gtgattttat tctggtagag aagtttgctt
    20941 atggcattaa agatcctatc taccagaaaa cgctgatcga aaccggtcat ccgaaacgcg
    21001 gcgatatcgt ggtctttaaa tatccggaag atccaaagct tgattacatc aagcgcgcgg
    21061 tgggtttacc gggcgataaa gtcacttacg atccggtctc aaaagagctg acgattcaac
    21121 cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc ggtcacctac tcaaacgtgg
    21181 aaccgagcga tttcgttcag accttctcac gccgtaatgg tggggaagcg accagcggat
    21241 tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat tcgtctttcc gagcgtaaag
    21301 agacactggg tgatgtgacg caccgaattc tgacagtgcc gattgcgcag gaccaggtgg
    21361 ggatgtatta ccagcagcca gggcaacaac tggcaacctg gattgttccg ccgggacaat
    21421 acttcatgat gggcgacaac cgcgacaaca gcgcggacag ccgttactgg ggctttgtgc
    21481 ctgaagcgaa tctggtcggt cgggccacgg ctatctggat gagcttcgat aagcaagaag
    21541 gcgaatggcc gactggtgtg cgcttaagtc gcattggcgg catccattaa tagccatctt
    21601 cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt tatgagataa atctcccgtg
    21661 gctaacgaca tcccccgtcg ttgtgtatag aatattcccc cgaagtttaa ggttggcacc
    21721 tccaggttgc cacggcacac gaaacagcgt tggtccccat ataccgctaa actgaaactg
    21781 cagcgaagca gttagcagag ccatgtatat caggtctgtt tcgtgtgctg aattgttgac
    21841 gcatttattt attggtatcg catgaacccc atcgtaatta atcggcttca acggaagctg
    21901 ggctacactt ttaatcatca ggaactgttg cagcaggcat taactcatcg tagtgccagc
    21961 agcaaacata acgagcgttt agaattttta ggcgactcta ttctgagcta cgttatcgcc
    22021 aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg atatgagccg gatgcgcgcc
    22081 acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg aatttgagtt aggcgagtgc
    22141 ttacgtttag ggccaggtga acttaaaagc ggtggatttc gtcgtgagtc aattctcgcc
    22201 gacaccgtcg aagcattaat tggtggcgta ttcctcgaca gtgatattca aaccgtcgag
    22261 aaattaatcc ttaactggta tcaaactcgt ctggacgaaa ttagcccagg cgataaacaa
    22321 aaagatccga aaacgcgctt gcaagaatat ttgcagggtc gccatctgcc gctgccgact
    22381 tatctggtag tccaggtacg tggcgaagcg cacgatcagg aatttactat ccactgccag
    22441 gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa gccgtcgtaa ggctgagcag
    22501 gctgccgccg aacaggcgtt gaaaaaactg gagctggaat gagcatcgat aaaagttact
    22561 gcggatttat tgccatcgtc ggacgtccga acgttggcaa atccacattg ttgaacaaac
    22621 tgctggggca gaaaatctcc atcacctccc gcaaggcgca gacaactcgt caccgcattg
    22681 tggggatcca tactgaaggc gcgtatcagg cgatctacgt cgatacaccg ggcctgcata
    22741 tggaagaaaa acgcgccatt aaccgcctga tgaacaaagc ggcgagcagc tctattggcg
    22801 atgttgagct ggtgattttt gtcgttgaag gcacccgctg gacgccggac gacgaaatgg
    22861 tgctcaacaa actgcgcgaa ggcaaagcgc cggtaatcct cgcggtgaac aaagtggaca
    22921 acgtgcagga gaaagccgat ctgctgccgc acctgcagtt cctggcaagc cagatgaact
    22981 tcctcgatat cgtgccaatc tctgccgaaa ccgggctgaa tgttgacact attgcggcaa
    23041 tcgtgcgtaa gcatctgcct gaagcgactc atcacttccc ggaagattac atcaccgatc
    23101 gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa actgatgcgt ttccttggcg
    23161 ctgaactgcc gtactccgtg accgtggaga tcgaacgttt cgtctctaac gaacgcggcg
    23221 gttatgacat caacggtttg attctcgttg agcgtgaagg gcagaagaag atggttattg
    23281 gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc gcgtaaagac atacaggaaa
    23341 tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt gaaatcgggt tgggccgacg
    23401 acgaacgcgc actgcgcagt ctcggttatg ttgacgatct ttaagagtca ctccgatgga
    23461 aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg agcgaaacca gcctgatgct
    23521 ggacgtcttc acagaggaat cgggtcgcgt gcgtctggtt gccaaaggcg cacgctctaa
    23581 acgctctact ctgaaaggtg cattacagcc tttcacccct ctcttgctac gttttggcgg
    23641 gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc tcactggcgc tgccattaag
    23701 cggtatcacg ctttacagcg gtctgtacat caacgaactt ctctcccgcg tactggaata
    23761 cgagacgcgc ttctctgaac tctttttcga ttacttgcac tgcattcagt ctcttgcagg
    23821 tgccactggt acgccagaac ccgcgctgcg tcgctttgaa ctggcactac tcgggcatct
    23881 gggctatggc gtcaatttta cccattgcgc gggtatcggc gagccggtag atgacaccat
    23941 gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc gtcgttatcg acaataaaac
    24001 gttcactgga aggcagttaa aagcgttaaa cgcgcgggaa tttcctgacg cagacacact
    24061 acgcgccgcg aaacgcttta cccgaatggc gcttaagccg tatcttggcg gtaaaccatt
    24121 aaagagcagg gaactgttcc ggcagtttat gcctaagcga acggtgaaaa cacattatga
    24181 atgatgagga ttgtcatggc tgaattactg ttaggcgtca acattgacca tattgcaacg
    24241 ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc aggcggcgtt tattgccgag
    24301 caggcgggag cggacggcat taccgtgcat ttacgtgaag atcgccgcca cattactgac
    24361 cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa tgaatctgga gatggcagtg
    24421 accgaagaga tgctggcgat cgccgttgag acgaagccac atttttgctg cttggtaccg
    24481 gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg ttgcagggca gcgtgaaaaa
    24541 atacgcgatg cctgcaaacg tctggcagat gccgggattc aggtttctct gtttatcgac
    24601 gccgatgaag agcagatcaa agctgcggca gaggttggcg caccgtttat cgagatccac
    24661 accggttgct atgctgatgc caaaactgac gccgaacagg cgcaagagct ggtgcgtatc
    24721 gccaaagccg cgacctttgc cgctagtctc ggtctgaaag ttaacgccgg acacggtctg
    24781 acctatcaca acgtgaaagc cattgccgcc atccctgaga tgcatgaact gaatatcggt
    24841 catgccatta ttggccgtgc agtgatgacc ggactgaaag atgcggtggc agaaatgaag
    24901 cgtctgatgc tggaagcgcg tggctaatgg caatattagg tttaggcacg gatatcgtag
    24961 agatcgctcg catagaagca gtgatcgccc gatccggtga gcgcctggca cgccgcgtat
    25021 taagcgataa cgaatgggca atctggaaaa cgcaccacca gccggtgcgt tttctggcga
    25081 agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg caccgggatc cgcaatggtc
    25141 tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg caaaccacgg ctacgactat
    25201 ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt aaatatgcat gtaacgctgg
    25261 cagatgaacg ccactatgct tgtgccacgg taattattga aagttaaaat aataccgttc
    25321 tctttaaaaa gtaaaacccc cgaactggta aggaacgggg gttttacttt ttaactttaa
    25381 ttttgtgcag catgcaccct caacaacagc gattatggat cagtgtactt atcttttaca
    25441 agcaacttgc aatctttagc ataaaaactc gagcctttac gaagaaagca atattgatgg
    25501 aaagattaac gtgaccgcca attcgtaagt acattaaaat tggcttcgtt attgaagatt
    25561 ttgctgtgct ttacaccatg ccacagaatt cccccattga aacgagtggt gtcgtcaaag
    25621 ctctggtgtg gagtgcagca tgcaccctca ataactcgca cgttcagttt tggggagatg
    25681 taagggctaa tctgaatggc tgcattcctt gtttaaggaa aaacgaatga ctgattgccg
    25741 atacctgatt aaacgggtca tcaaaatcat cattgctgtt ttacagctga tccttctgtt
    25801 cttataacac aaggaaacgt acttaaggtg cgtccggtga accaatcgga cgcgccttta
    25861 ataactataa ataagtgtct tggcagatac tatataaatt aacttagtga atgattatgc
    25921 taatgtcatc aattaaataa atataatggc gttaaggctt cccagtaata taattaatac
    25981 tctacttcca gagtagaata ttaaatttta tccgcgtggt gcatcagcac aaatttatcc
    26041 cacaactgtt cttctgtctc gacatgcgcc ggatctttca caatagtatt ggggatcggg
    26101 cacaccttct ggcaggttgg tgtctcgtag tgccctacgc attcggtaca cttatcgctg
    26161 ttaatttcgt agatatgatc tcccattgaa atcgcctcat tcgggcattc gggttcacac
    26221 atatcacaat taatacagcg tttagtaatt agtaaagaca tttcaatgga ttaccattaa
    26281 atcattttaa aatcagtaag ttgtatcgag tttgtatgct ttactgtcat taacttactg
    26341 tatgttgatc cagtgtattt caccttgata aactcagtcc agcaacacaa aaccgcaaca
    26401 cattgcattt tgtcccgtag aaaagacttg tatgtgtgag cttgttttct gcgcctacgc
    26461 agataaggat tgagaatgcc gcgcactgta acacataatc cggatagccc caataatgac
    26521 gatgttttag ccgcatctga aaaatgggac gcctgtaaac ccccctatac cagcgcacac
    26581 atgaaaatct gtgttgctgc cgccaaaatc atcctcgctg cttccggcgt ggctcgccgt
    26641 tccaaatacg aaaaagagaa ctatctccgt atcgatttca gcaaagccgg taaggttaca
    26701 ttttacgccg agtttccaaa aaagatgggc ctcaaaggta aaaagctcgg cgagtggccg
    26761 gagctcgcta tccagctggc gcgcgaaaaa gcactaggta tggctgacgg tggactgcgg
    26821 gcagagtccg tacatgcagc gctggaaatg taccgggatg acctcaaagc caaagtagcc
    26881 cggcagaagc tgagcccgga cagtttcaca acctacgggg tgcgtatcga ccggattaaa
    26941 gcaacgttcg gcgagcgtga ggtgttcagc gacgtaacat acaatcggct ggtggaagtg
    27001 ctggacgagt ggatcgccac tcgctcgaac aataacgccc tggagttgtt tgccgagctc
    27061 cgtcggttct ggaagttctg cgcacctact ctttgcaacg gccgcaatgt tgccgccagt
    27121 ctgccagatg attatgtttc cttccgcgta cagaaaccta cccccacacg gctttttacc
    27181 gatattgaat caatcgcccg actctggctc aatgttgctg cctgcacctc tgtacaccag
    27241 aagaatgctg ttcgcttcat gatcatcact ggtgttcgtc cgattaatgt ccataacctg
    27301 cgctgggact acgtttacga ggaggctggt gaaattgttt atccggaagg ggttatcggc
    27361 atgcgagggg ctatgaaaac acgccaggct ttccgcctgc cgataacgcc tgagatccgg
    27421 cggattatcg acgagcagaa agcctggcgt gattcagttc ctgagtgcaa cagggattat
    27481 gtatttttgc agccacgtga tccaatgcag ccattttcaa aacgatcact ggataagctg
    27541 gtgaaaacat acagcccgga cggggctgta a
//
LOCUS       DADMFL010000051        27037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.051, whole genome shotgun sequence.
ACCESSION   DADMFL010000051 DADMFL010000000
VERSION     DADMFL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.051"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..437
                     /locus_tag="JNZ27_002129"
     CDS             <1..437
                     /locus_tag="JNZ27_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8664461.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNZ27_002130"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNZ27_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY8664462.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="JNZ27_002131"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="JNZ27_002131"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY8664463.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="JNZ27_002132"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="JNZ27_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY8664464.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="JNZ27_002133"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="JNZ27_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY8664465.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNZ27_002134"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNZ27_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY8664466.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="JNZ27_002135"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="JNZ27_002135"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY8664467.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="JNZ27_002136"
     CDS             complement(7412..8227)
                     /locus_tag="JNZ27_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8664468.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="JNZ27_002137"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="JNZ27_002137"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY8664469.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="JNZ27_002138"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="JNZ27_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY8664470.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="JNZ27_002139"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="JNZ27_002139"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY8664471.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNZ27_002140"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNZ27_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY8664472.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDLAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="JNZ27_002141"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="JNZ27_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY8664473.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="JNZ27_002142"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="JNZ27_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY8664474.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="JNZ27_002143"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="JNZ27_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY8664475.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="JNZ27_002144"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="JNZ27_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY8664476.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="JNZ27_002145"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="JNZ27_002145"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY8664477.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNZ27_002146"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNZ27_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY8664478.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="JNZ27_002147"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="JNZ27_002147"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY8664479.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="JNZ27_002148"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="JNZ27_002148"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY8664480.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNZ27_002149"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNZ27_002149"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY8664481.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNZ27_002150"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNZ27_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY8664482.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="JNZ27_002151"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="JNZ27_002151"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY8664483.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="JNZ27_002152"
     CDS             24262..24876
                     /locus_tag="JNZ27_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY8664484.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="JNZ27_002153"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="JNZ27_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY8664485.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="JNZ27_002154"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="JNZ27_002154"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY8664486.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6202 a         6886 c         7447 g         6502 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccttgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gcctcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccg
//
LOCUS       DADMFL010000052        26243 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.052, whole genome shotgun sequence.
ACCESSION   DADMFL010000052 DADMFL010000000
VERSION     DADMFL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.052"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..488)
                     /locus_tag="JNZ27_002155"
     CDS             complement(<1..488)
                     /locus_tag="JNZ27_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664487.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWCSVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFI
                     LV"
     gene            complement(589..1188)
                     /locus_tag="JNZ27_002156"
     CDS             complement(589..1188)
                     /locus_tag="JNZ27_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664488.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(1360..2298)
                     /locus_tag="JNZ27_002157"
                     /pseudo
     CDS             complement(1360..2298)
                     /locus_tag="JNZ27_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2361..2735)
                     /locus_tag="JNZ27_002158"
     CDS             complement(2361..2735)
                     /locus_tag="JNZ27_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY8664489.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(2739..3887)
                     /gene="wzy"
                     /locus_tag="JNZ27_002159"
     CDS             complement(2739..3887)
                     /gene="wzy"
                     /locus_tag="JNZ27_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY8664490.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSMTNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(3907..4776)
                     /locus_tag="JNZ27_002160"
     CDS             complement(3907..4776)
                     /locus_tag="JNZ27_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY8664491.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(4805..5695)
                     /locus_tag="JNZ27_002161"
     CDS             complement(4805..5695)
                     /locus_tag="JNZ27_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY8664492.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLFRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(5725..6981)
                     /gene="wzx"
                     /locus_tag="JNZ27_002162"
     CDS             complement(5725..6981)
                     /gene="wzx"
                     /locus_tag="JNZ27_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY8664493.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(6997..7542)
                     /gene="rfbC"
                     /locus_tag="JNZ27_002163"
     CDS             complement(6997..7542)
                     /gene="rfbC"
                     /locus_tag="JNZ27_002163"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY8664494.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(7547..8425)
                     /gene="rfbA"
                     /locus_tag="JNZ27_002164"
     CDS             complement(7547..8425)
                     /gene="rfbA"
                     /locus_tag="JNZ27_002164"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8664495.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(8484..9383)
                     /gene="rfbD"
                     /locus_tag="JNZ27_002165"
     CDS             complement(8484..9383)
                     /gene="rfbD"
                     /locus_tag="JNZ27_002165"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY8664496.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(9383..10468)
                     /gene="rfbB"
                     /locus_tag="JNZ27_002166"
     CDS             complement(9383..10468)
                     /gene="rfbB"
                     /locus_tag="JNZ27_002166"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8664497.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(10841..11734)
                     /gene="galF"
                     /locus_tag="JNZ27_002167"
     CDS             complement(10841..11734)
                     /gene="galF"
                     /locus_tag="JNZ27_002167"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY8664498.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(11909..13303)
                     /gene="wcaM"
                     /locus_tag="JNZ27_002168"
     CDS             complement(11909..13303)
                     /gene="wcaM"
                     /locus_tag="JNZ27_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY8664499.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(13314..14534)
                     /gene="wcaL"
                     /locus_tag="JNZ27_002169"
     CDS             complement(13314..14534)
                     /gene="wcaL"
                     /locus_tag="JNZ27_002169"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY8664500.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(14531..15811)
                     /gene="wcaK"
                     /locus_tag="JNZ27_002170"
     CDS             complement(14531..15811)
                     /gene="wcaK"
                     /locus_tag="JNZ27_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY8664501.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(15991..17469)
                     /gene="wzxC"
                     /locus_tag="JNZ27_002171"
     CDS             complement(15991..17469)
                     /gene="wzxC"
                     /locus_tag="JNZ27_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY8664502.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(17471..18865)
                     /gene="wcaJ"
                     /locus_tag="JNZ27_002172"
     CDS             complement(17471..18865)
                     /gene="wcaJ"
                     /locus_tag="JNZ27_002172"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY8664503.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLSGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(18920..20290)
                     /gene="cpsG"
                     /locus_tag="JNZ27_002173"
     CDS             complement(18920..20290)
                     /gene="cpsG"
                     /locus_tag="JNZ27_002173"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY8664504.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(20395..21831)
                     /gene="cpsB"
                     /locus_tag="JNZ27_002174"
     CDS             complement(20395..21831)
                     /gene="cpsB"
                     /locus_tag="JNZ27_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY8664505.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(21834..22964)
                     /gene="wcaI"
                     /locus_tag="JNZ27_002175"
                     /pseudo
     CDS             complement(21834..22964)
                     /gene="wcaI"
                     /locus_tag="JNZ27_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(22961..23440)
                     /locus_tag="JNZ27_002176"
     CDS             complement(22961..23440)
                     /locus_tag="JNZ27_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY8664506.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(23440..24408)
                     /gene="fcl"
                     /locus_tag="JNZ27_002177"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(23440..24408)
                     /gene="fcl"
                     /locus_tag="JNZ27_002177"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY8664507.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(24411..25532)
                     /gene="gmd"
                     /locus_tag="JNZ27_002178"
     CDS             complement(24411..25532)
                     /gene="gmd"
                     /locus_tag="JNZ27_002178"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY8664508.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(25558..26106)
                     /gene="wcaF"
                     /locus_tag="JNZ27_002179"
     CDS             complement(25558..26106)
                     /gene="wcaF"
                     /locus_tag="JNZ27_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY8664509.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(26122..>26243)
                     /locus_tag="JNZ27_002180"
     CDS             complement(26122..>26243)
                     /locus_tag="JNZ27_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY8664510.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         7177 a         6527 c         5605 g         6934 t
ORIGIN      
        1 cacacaagga taaaatatcg tcccaacttg taatttgtaa tgttgatatc ttttattaaa
       61 cttgcaattg agatcgcaac cagcaatact gacaatgggt aaatgggtga taaatacctg
      121 tcaccttgcg taacagaaca ccaaaataag acatatgtta aaacaatcga gaaaaatacc
      181 caaagtattt tattattcct aagtgtaaag acagacagta tagcataaaa aacaacatca
      241 ctgccaactt tattaattgc ttgttgcaaa tctaatgtgg caggacttat accatgtcga
      301 gcttgttctg ctgtttgaac ttgtagatca atttcattcc ataggtagtg accaaatata
      361 tttcctccag caggactaaa aggatcacca gagatccaaa aactacggac ataccaataa
      421 atgccaccaa gaattgttgc caagctaaaa ataagaaatg tctgtatgtt tctctttttc
      481 gctatcaata taacaaatat gatggctcca tatatggcac caaatagttt tacgccacaa
      541 gcaattccaa taaaaaaacc acataaaaaa attaattcat tttgtgtttt attgttttct
      601 gagcgaatat atataagaat actggctgca gcaaaacaaa gatagcgtta aatcaatata
      661 tgcataacca agatgatctt taagtggatt gatagtaaat agtaggattg tagcaataat
      721 gctaggataa taagttttcg taatttccca tgaacagcca acaagtccac aggctattac
      781 aaaaaaacag atatttgcta aaaactgagc taaccttaca tctcctaact gtaaacctaa
      841 agccattaaa agatctgcat tttgcgggaa cagaggaaaa cgcaaatatt gttcaacaac
      901 aattgcatgg tgctcaacta atgaacgagc caaaggtaag tgatacatag tatcatccca
      961 atcaccggga gctcgcaggg acgataacaa tgtaaccaca aaaatcaaaa caagaaataa
     1021 aataacaaac agcgtactga actcaatatt atttattgcc agccatctaa attgctgacg
     1081 tttaataatg cttaaaaccc aacacaccgt aaaaattaaa aagatagcaa tcttattaaa
     1141 ttgcccgacc atgcccagaa agtaaattat caaaatattt aaagacatcc ctatgcatag
     1201 gcaataaaaa aataaagaaa atttgttaag tcctgccacc ccctccttaa aaaaagatga
     1261 caatacaata ttaccaatga caaaaaatgt cattccaaaa gcaaaacaat acacaacata
     1321 caaatacaac ggcaattcaa tcatagtaaa atactctatt tacattatca gtttatagtt
     1381 tttttcagaa agataaaata atgccttcat ttcacgtcta gttgttgtat ttgaatgcaa
     1441 aataatgcca catacaaatg ccattattga cgataacatg atggaggcag ttaaaaaggc
     1501 agtcggaaat cttctcacca aacctgtatc aatataatca acaatgatgg gcaatcctaa
     1561 aattatagac aaagcagcaa gtaaaacgcc gataatacta aaaaagtata gtggccgttc
     1621 aagcgagtaa agtttaatta ttgttttcag aatcttgaac ccatcactcc aagttgatag
     1681 tttgctcaca ctcccttcag atcgctcgcc atattttgta ttaacctcac catacttcat
     1741 acgtaattct aaagcatgaa ttgtaagctc agtttcgatt tcaaatcccc ttgatagaca
     1801 tggaaaagat ttaacatagc gtctggaaaa tacacgatac cccgacaaca tatcagtaaa
     1861 attcccttta aagatagtct gaacagtacc agtcaacagt ttatttcctc agcgatgccc
     1921 ttttctgtat gtattttgat ctccattctc ttgacgacat cctacaacca tatccaattg
     1981 attttttatg agatggttaa tcatttctgg agcagaactt gcatcataag tgccatctcc
     2041 atcaaccatt agataaatat ccgcatctac atctgagaac atacgtctta cgacattccc
     2101 cttgcctttc agaggaacag agtgaactgt tgctccagca tcctctgcca cttgtgctgt
     2161 actgtcagtc gaattattat caaatacata tatagaagca gttggaatat tcgttttgaa
     2221 atcatttatg acttttgcta ttgccttctg ctcattataa caaggaataa ttacagcgat
     2281 attaaaagag tgatacataa gcccctcata taaactattt agctaatgtg caagtaattg
     2341 tgtttttaac aaatcgttta ctaacgaaaa atgaaatatt ttgatgcgca atatcctaaa
     2401 accaaactaa aaagagaaaa ggtgaacaat gtaaatattg gagataaagc taagagatca
     2461 aataaaacgc cgaataaata actcatcgcc cccataaaaa atataaatat aaaatatcta
     2521 gccttggttg cagatacttt aaatgagcat cttgcatttg caaaaaaaga aaaggttgct
     2581 gcacatataa aaccaacaat gttacttcgg ccttgaccaa aagctaaatt attataacac
     2641 gcataaaata ccccccaatg taaagcagta ttgacaattc caattaaaaa ataactaaaa
     2701 aaagaactgg tcaataactt ccctattttt aacatccttt attttgctcc agaagtgagg
     2761 ttattactaa tttggatatt ttctatagaa aatacccata aaactacaac gcctataata
     2821 aaaattccca tgggtgtaaa aagaaatgga ttagtcatag aaacggtaat actgaaaaaa
     2881 agtattaacg acatcattaa tgatgttttt gtactattta aaaaatagcc tttcaaaaag
     2941 ataagcaaca taggaactaa tgatataaat acaccaataa cgcctgtttt ctgaagtatc
     3001 tcaagaggta ctatttcaat ccttaatcga tctaatataa atgaaccaaa tcctcttcca
     3061 aacaagaacg tcgctaaatc aacattttta taataaaaat ataaatcatt aaatctaaca
     3121 gagtcagaat cttcactgcg tgatccgacc atgtaaagca taattataaa tagaacgctg
     3181 cccaatatta ttataatagt atttaaatat agcttatgta atttaaaact ggctaataac
     3241 agtgataatg ttgtaaaaag taatacacct cttgattctg tcaataatat cgcaagcact
     3301 attatgagac attttaaata ttttttgtcg acaaaactga taatcgcacc gacaccaaaa
     3361 aagtagaagg atttggaaaa aaaagcccca tcgggacgaa aaaagaactc gctagtcaat
     3421 gataagtgtt cataaattaa attaaaattt aacaaaccga aattcaacaa aattatgtat
     3481 gaaatatata acagtgatcc atataatgca aatgcattaa ctattttaat aaaaatctca
     3541 cataaaaata cttttaaagt atatcttagt gtaaatagat atataagtga cagcataaaa
     3601 aggtatggtt gcacatcaaa cagtatatca tttattttat taccagctaa taagccgaca
     3661 aagaagccat aaaaaggaaa taaaagcaac catataaaaa gactgacaca tttttgggtg
     3721 attgaaaaat tgaatgtaac taaattaaaa attaaaagta taaaactaaa tacaaataat
     3781 aaatatcgca aagggaatat tcccaatggc tccagtaatc ttccaccacc accaataatc
     3841 agttcaatac ataaaaatgt tataaaaatt ttatttatat tattcatatt cgtaaggtga
     3901 tgttttttaa aatatgttta aaatcaacat taataaaata taaattttat cagccttttt
     3961 ccgccttaac ttccaaaaac ttaaagccaa gatcaaattg cctatataac ctttacccaa
     4021 ttgattattc acaaagctat catcagctaa ggcattagct attttagtta cttcttttcg
     4081 ataccacttg tttctcacca aaccaagccg tttgtatttt gctttaaaac taatgtttgc
     4141 tcccacttgg ttgttttcat gctgtctgta cagcattgtt ggttttctat caatatacca
     4201 cgaataattt ctcgtccgcg caaatgaata taaaaaccaa tcatgcagac agatatcttg
     4261 tgcagcattt ttattattta tgatgaactt cttaaattca ataagcgttt ctttttttat
     4321 aacatatgta caccccggac ctgctgcttc aaaaaaataa tcgaattgag tttgcgactg
     4381 agccttatca actaaatttt tttgccctga tggataatat gcaattacat tgctggaata
     4441 acaattcgca ttattaaata ccaagcagtc aattgcattt tttattttat ctttatacca
     4501 aatatcgtcc tgatcactaa aacagatata atcatagttt tcaagatcga catctttaat
     4561 taatctataa aaatttttac ctgccccccc aaacttattg acgcttggaa gtatatttat
     4621 aattgaggga tatgataatt gaaattcttc acaaatattt aatgtattat cagttgataa
     4681 atcatctgat ataaaaattg agatgtctac atctttttgt tcaataatac tttttaattg
     4741 ttcctcaatc cagcattcgc cattatacgt agccataagt acagctactc ttttttgatc
     4801 agaactatgc attataacga ccgcccccag taattcctct tattccatcc agcgtagcca
     4861 aaatatgttt tttcatatat tcaattttat ttttttgagt tgcgataatt aaagtcgtat
     4921 gaattaaaga aaaaacaact tccctaatag agagtaaaag aggcacatga ttttttctaa
     4981 acaaaagaaa tgcatttctt actcgataat aacgacgtac tggcgagtga attggtatgt
     5041 taatcccaca gatttttact gacttatccc caatagcatg aaccatttct atatcaggaa
     5101 tgatatgaac taatatgcca tagtttaagc atctaagaca ccattctgta tcgacataat
     5161 caataaaaag ttcctcatcc atcattccaa caatctcaaa aacttctttt gaaaccatgg
     5221 ttccggatga gattgttact gaacttttaa acggtgtctg tccttcagtg acatgaattt
     5281 tctctcgaag accatttttt ttgatattac aaataggata ataatgtgac ttatctctat
     5341 caataaaaac tgggccagct atttttgcat tttcattatc catatagcag gaaagttttt
     5401 tgataaactc attgcgaata cggctatctt gatcaaaaaa aattatcttc tctgcccctt
     5461 tttcaagaga atatcgaatc ccaatatttt gtgcttttgc tataccttca ttcctgccaa
     5521 gacaaattaa atgaactttt tcaatgtctg ctaatttatc tttcaaggtg aaattagtcc
     5581 tattatcgac tactacaacg taacaatttt gctcctttag ttcagtgatc agtgcattca
     5641 aatttttaag ttcgggatta tatgtcacaa tgacagcgta aatattacta ttcattatcc
     5701 aagtgactca gtaattggtt aattttagtt ttcatataca gaacacattc tagtataaag
     5761 ccattgtaat aacagagcat ctaaaattac acgtatgctc caggatattg cggcacccag
     5821 taacccatat ttttcgacca taaaatacaa aagggctaaa taaggaacca attcagcaca
     5881 atgaatcaaa gcagtaattt tagattttcc taatgcctgg attgcagaga atggtatcat
     5941 tgccagcgca ttgaagaaaa aaccgatcaa taacacattt aatatattaa aggaattaat
     6001 ttggtaattc tcaccaaacc atattttcaa tacaagccct gaatataaca agcctatgat
     6061 tatcaccggc aaacagatta gaaacattaa aagcaatgat ttatttacat tacgtttaaa
     6121 ttctttaaag tctttgatgt tacttaacct tggaaacact gccctcccga ttgctgcagg
     6181 tattattcct aattttagaa taacctccgc tggtgcagaa taaaatgcaa ctttatctgc
     6241 ccccataata tttgacacta taaatcgatc aaaatacacc atgaccggac ttatgatatt
     6301 acttactgtc atccaaccgc caaagaaaaa gagtcgttta aaggttttat aacaaaaatg
     6361 aacccccgaa attttaatat cattcctgac ataataggca gagactaaaa tcgaaataac
     6421 tcttgcaaat attaaaccag caaccgctgc cgacaatgta gcactataaa aaacaaatat
     6481 agccggaatt cccgcgatgc aagagcttga tatagatttt tgaatattta caatgccaaa
     6541 tttttcatcc ccctcaagaa tggctgacca taattgattt agaataaata atggaatgca
     6601 aatagccaac agtttaaatg ctagttgtat atcactatgt tcaacaccag aaatttttag
     6661 ataattaaca attccatcag agaaaatcaa taataaaaaa gcaccaaagc atgaaaatag
     6721 caccaaaaaa gatgtacttg ttgaaattac cttggttctt tcatgatgat tatcacgatg
     6781 aattgcaatt tctctaataa cagagcgagt caggcctaca tcaaaaattc cagcataccc
     6841 aacaagtgca attgcaattg tataaacacc gaaaccttca ggccccaaac ctcgagcgag
     6901 aaatcctagc gaggggatag caattaaagt tggtattgca tagccaaaaa ggttccagac
     6961 actatttttt attatgctca ttattacatt tctttatcat ataaaataat cagcatctac
     7021 aaatttttga ccattaatat ctttatcaga aagcaatgga ttttgaaccg gccattctat
     7081 atttatactt ttgtcgctcc ataaaatccc acgctcttgc atttttgaat agaaattatt
     7141 tgttttgtag acaaattctg cttcatcact aagaacgcaa aacccatgag cgaatccttc
     7201 cggtatccat aattgacgct tattctccgc cgacaaattt acaccaaccc acttgccaaa
     7261 tgttggtgat gatttccgaa tatcaacagc tacgtcaaaa acttcaccga caacacagcg
     7321 taccagtttt ccttgagcat aaggttccag ctgatagtgc agtccgcgta acacaccctt
     7381 agttgattta gaatggttat cctgaacaaa ctcaaccttt cgtcctacag cctcttcgaa
     7441 aactttctga ttgaagcttt ccataaaaaa gccccgctca tcaccaaata cttttggttc
     7501 aaaaattagc acatcaggaa tttcagtttt aattacgttc attttattaa taacctttaa
     7561 tcattttcag cagatactga ccataagcat ttttcttcag cggctcggct aataccttca
     7621 cttgctcagc atcaataaag cctttacggt aagcaatttc ttccgggcag gaaactttca
     7681 gcccctgacg ctcttcaatt gttgcaatga agttgcttgc ttcaataaga ctttgatgtg
     7741 tccccgtgtc cagccatgca taaccacgcc ccatcatagc gacagacaaa cgcccttggt
     7801 ccatataaat acggttaata tcggtaattt ccagttcgcc acgtgcagat ggtttaaggt
     7861 ttttcgccat ttctacaacg tcattgtcat agaaataaag cccagtaacc gcatagttac
     7921 tttttggttc cagcggtttt tcttccagac taattgccgt accgttatca tcaaattcca
     7981 ccacaccgta gcgctcagga tcatttacgt gataggcaaa aaccgttgcg ccactctctc
     8041 tgttaacagc ggtatccatt aatttcggca ggtcgtgacc atagaagata ttatcaccaa
     8101 gtaccagcgc acaatcatca ccacctataa attcttcacc aatgataaat gcttgcgcca
     8161 gaccatccgg actcggttgt actttatatt gcagattaag cccccactgg cttccatcac
     8221 caagcaattg ttgaaaacgt ggtgtatcct gcggcgtact gataatcaga atatctcgaa
     8281 tacccgccaa cataagcgtt gaaagcggat aataaatcat cggtttatca taaatcggta
     8341 gcagttgttt gctcacagcc attgtcacag gataaagacg agtgccggaa ccaccagcca
     8401 gaataatacc tttacgcgtt ttcatttcac tattcctttt aattcatctc gctctggcat
     8461 catgagcgag atgcaataat ttgttaaatt gccgtggtcg taaataattc gttgagcata
     8521 cgtttcacgc ccacctgcca gtcaggcaag acaagcgcaa agttctgctg aaatttttcg
     8581 gtattgaggc gagaattatg gggacgacgt gctggtgtgg gataggccgt tgtcggcacg
     8641 gcattaagtt tgttaagagc aaggttgatc cccgctctgc gcgcttcttc aaataccaga
     8701 gcggcataat cgtgccaggt tgttgtacca ccagcaacca gatggtacaa accagcaact
     8761 tctggtttat ttgcggccac gcgaatggca tgagcggtgc aatcagccag taattcagcc
     8821 cccgttggtg cgccgaattg atcgtttatt acagccaact cttcgcgttc ttttgccagg
     8881 cgcaacatcg ttttggcaaa gttatttcct ttaccagcgt atacccagct ggtacggaaa
     8941 ataagatgct ttgcgcaatg ttcttgtaac gctttttccc cagctagctt ggtttcaccg
     9001 tagacattta gcggtgcggt tgcatccgtt tccagccatg gcgtgtcacc gttgcctggg
     9061 aaaacataat cagtggagta gtgtataacc caggctccga cttcattagc tgcttttgca
     9121 atcgcttcga cgcatgtcgc gttaagtaat tgcgcgaaat tcggttctga ttccgctttg
     9181 tctactgcag tgtgagcagc agcattaaca ataacgtcgg ggcgaatttt tttgacggtt
     9241 tcagccacac cttcggggtt gctgaaatct ccacaataat cagtggagtg aacatcgagc
     9301 gcaatcaagt tacccagagg agccagagca cgctgcagtt cccaacccac ctgccctgtt
     9361 ttgccgaaaa gcaggatatt cattactgac ggccctcata gttttgttca atccacgact
     9421 gataagcacc gcttttcaca ttctcaaccc aatttgtatt agccagatac cattccaccg
     9481 ttttacgaat cccgctctca aacgtttctt gcggtttcca gcccaattcg cggctaattt
     9541 tatcggcatc aatggcgtaa cggcggtcat gccccgggcg atcggcaaca taagtaattt
     9601 gctcacgata agatttctct ttcggtacaa tctcatccaa caaatcacaa atagtgagca
     9661 ctacgtcgat gtttttcttt tcgttatgtc caccaatgtt ataagtttca cccgctttac
     9721 cttcggttac gacggtatat aacgcacgag catgatcctc tacatacaac cagtcgcgaa
     9781 tttgatcccc tttaccataa ataggtaatg ccttaccttc cagagcattc agaataacca
     9841 atggaataag tttttccggg aagtgataag gaccataatt gttcgagcaa ttagtcacaa
     9901 tggttggtaa accataagtc cgcttccagg cacgtaccaa atgatcgctg gaagctttag
     9961 acgcagaata cgggctactt ggcgcgtaag ctgtcgtttc cgtaaatagc ggtaacgctt
    10021 cgttattatt tgcttcatcc ggatggggta agtctccata aacttcatca gtagaaatat
    10081 gatgaaagcg gaaacttttt ttcttttcat cattcagagc agaccagtaa ttgcgcgccg
    10141 cttctaaaag aacataagtc cccacaatat tggtttcaat aaatgccgca gggcccgtta
    10201 ttgaacggtc aacgtggctc tctgccgcca ggtgcatcac cgcgtctggc tggtgctgtg
    10261 cgaaaatacg cgacatggct acagcatcgc agatatctgc atgctcaaaa gcataacgtt
    10321 cagaatcaga aacatcagcg agtgattcta ggttcccggc gtatgttaat ttatcgacat
    10381 taacaacact atcttgcgta ttatttatta tgtgacgaac aacagcagaa ccaataaacc
    10441 ctgcgccacc agtaacaagt atcttcactt ttttattcca tatagctata gagcatgctg
    10501 tgaaatagac tgctccagat ttgattaata gaggtattaa tacacgctac cgcccctggc
    10561 ttaacagcta ccagtgcact gcatgcatct caacgatgtg gcgagcgtta gccactcgcg
    10621 ccaaatccga aaaattcaaa cgttaattgt cttaccaaac agccgtagaa acaaggaaaa
    10681 tcctggaaaa atttgaataa tatgagctat gtaacttctt gtttttatag gctttataaa
    10741 aacggcaacg gcaggaaaat tcaatgagca aaacttatgc cacgaatctt cactgccgtt
    10801 ataattttct tatcaaccgt tacatccggt cagattttca ttattcgctt aacagcttct
    10861 caataccttt acggaacttc gccccttctt tcaggttgcg taggccatac ttcacaaacg
    10921 cctgcatata gcccattttt ttgccgcagt cgtaactgtc gccggtcatc agcattgcat
    10981 caacggattg ttttttcgcc agctcggcaa tagcatcagt cagctgaata cgtccccatg
    11041 caccaggctg agtacgttcc agttccggcc aaatatcggc agaaagcaca tagcgaccta
    11101 cggccatgat gtctgagtcc agcgtctgcg gctgatccgg tttttcgata aattcaacaa
    11161 tacggctgac tttaccctca cggtccagcg gctctttagt ctggatgacg gagtattcag
    11221 agaggtcacc cggcatacgt tttgccagca cctggctgcg gcccgtttcg ttgaaacgtg
    11281 caatcatggc agcaaggttg taacgtagcg ggtcggcgct ggcatcgtcg atcacaacgt
    11341 ctggcagtac cacgacaaat gggttgtcac caatggcagg tcgcgcacac aaaatggagt
    11401 ggcctaaacc taaaggttcg ccctgacgca cgttcataat ggtcacgccc ggcggacaga
    11461 tggactgtac ttccgccagc agttgacgct tcacgcgctg ctcaaggagt gactctaact
    11521 cataagaggt gtcgaagtgg ttttcgaccg cgttcttgga cgcgtgagtt accaggagga
    11581 tttctttgat ccctgcagcc acaatctcgt caacaatgta ctgaatcatt ggcttgtcga
    11641 cgattggtag catctctttg ggtatcgcct tagtggcagg caacatatgc atcccgagac
    11701 ccgctacagg aataactgct tttaaattcg tcattatttc atccacctgt aaaatggttg
    11761 ctgaattata gcttgttcga tttttttcgc cagcatcaat taccctgaat tgattactga
    11821 attacttgtg atgttacgcc gcttcgttgt ggattgcagt agcattgttc ctaagtatgg
    11881 ctccattttt ccaggaatgg tcgcaaatct actccctccg ttccggcaat ctaaagttaa
    11941 tcttttccac attaacaata tggtgattaa tcctgtcgat atcgacggag ctttgccctc
    12001 tttcattcac cgcatgaaca tttgcaagag acagcagtgt ttcttttttc gccataaaga
    12061 cgccgcgaac gtctttgcgc atgtcgaagt tcatgctcaa tgctggtcca actgaggatt
    12121 cctgcatcac attgatattt cgtataaaaa gatgttgcgg cttgttgtgt aactccagcg
    12181 aggcacgctt catctcaatg ttagttagcg acacaaagga gacggcattc ccggcggaga
    12241 tttggatgcc gcgcaattta taagcaagat gggtattatc cagttgaata ttattcactc
    12301 ggaaattttg cggtatcgag agatatttgc ctttaattac cccatagccg attaacatcc
    12361 cggcgctatt aatcatttca atattatcaa tcacgaaatt gtcacaaccg taaatagcga
    12421 ctgtcgcgtt atcaatgccc gctttcttac tgaaatccgg cgtgatattg cgggcattga
    12481 tattacgaat aacaaaatgt ttaccatttt caacatggtt caactgccga caatccgatc
    12541 ccgtgatatt cgccacgaca aagtttttca ctgcctggtc ttccgggtag ttgttatcat
    12601 aagtgcttcc cgcaaggcct atgccgatgc cccagttgat tttgccgttg gtacagttga
    12661 tacgctcgat gacatggtca gatatcaaaa tatcactgtc gttaattgcc acgttccatt
    12721 caatggcgtc gccctgtaag tcgctgaact tacagttggt aatgttggca ccgataatct
    12781 ggttatgaaa tccctggcgt aagatggcgt aattagcgtg gctgacagtg aggttatcga
    12841 tggtcaggtt gcgcatgacc cgtttgtttt tgccgccaat ataaatctgc attaccgggc
    12901 caaagccgct catcgccagc cctttgatgg tgcagtcaga accacgcaca tccagggtaa
    12961 tgttatgcat accgccgccc tctccccctg tcacctggct gccgtcctgt aagacaaatc
    13021 gccctctgcc gttgccgcgc aggcttccaa ggatgtgtaa cgttttgccg ggagggatga
    13081 agatgccggt gttgatattg tcacaaacga atccggcagg cacgacgacc gtttgccctt
    13141 cgctgaaggc ttgtttaaat gaggcgatcc agtcatgtgg gttgtagtcg ttaatgttaa
    13201 cgctttgtcg ggcgggaagt gcgcgagcga aaggggtatg gaggaaggca agcgccgagc
    13261 ttgccgtcag gaaggtgcgt cgggagagtg ttttaaatgg catacgttct cctctataaa
    13321 gcctgcagca agctggcgag ttctcgatta atcacctgct ggttaaaatc gtgttcgact
    13381 ttttcgcgcg cacgtttgac gaccggagcc agttcgtcgg tatccagttg gctaaacgcc
    13441 gccagacgtt gtgccagtgc gcgagcatcg ttctcaggca ccagccagcc ggatttatcg
    13501 gcctccacca gttcaggaat tccgctatgc agagtagaaa ccaccggaat accgaccgcc
    13561 atcgcttcca ttagcgccac cggaatgcct tccatatcgc catccgcgcc cgttaccgat
    13621 ggcaacagga agacatccgc gtcgtcgagc atctccttca cttcatggct cggtttaaag
    13681 cccggcatct ctaccacatc ttccagttga tattgttcga tgagggtgcg caggcgtcgt
    13741 tcccacaggc caatgccgag gatgcgatag cgaaatgcca cgccctgctc tttcaactga
    13801 cggcaggctt caatcgccac atgcaggcct tttttctcgg ttaagcgtgc gacagatatg
    13861 atttccagcg gcgttgcggg cgctttcacc ggacgcgggc taaagcgcgt catgtccacg
    13921 cccatacgcg atacggcgat tttttctctg gggcagccca ttttttgcag ccttccggcc
    13981 cacagatcgc ttatcggtaa catcaggtcg ccacggcgaa acagttgttg atattcggga
    14041 gtgtagtggt tgagcacttc ccgactggag atatcaatac cgtggaagat ggtggcaatt
    14101 ttgccgcgaa tgatacccag ttcgcgtagt tttgctgcgg ttacccccgc cgggccaaaa
    14161 tgagcgatga acacatcggc gcgaaacggt gttgcgacct gaccacaaat ggcagacaaa
    14221 atcaggttcc gcgactcggc accatagcgt ttgaggttga gcgcatgcca ggtattttta
    14281 cgatgaatgc cacgtaaggt ctggctggcc cggtggcgca gtttcgctac tttgcccgca
    14341 ggttcgtcct gtaaccagcg ggttctggcg acaaggttgt atttcgtcca tgccgcgtgg
    14401 gtgttctctg tatcgccttt ttgcaaagcg acaatctcca cttcaaagcc catatcaata
    14461 aacgcggtaa tttgattgag gacgaaggtt tccgacgaca gcgggaattt caataagaag
    14521 aagccgacct tcatttcacc tccccgatgc gctcaagcac agactgcacc atctgcattc
    14581 ccgtctgacg ttcgcgactg acggcttcgt taaatcgagc gttcagcacc ggaagctggc
    14641 ctaaggtatc cgcaaccatc gcttgcaggc tgccgtccag taaatgacgg atatcaatcg
    14701 ccatctctgg cagtcccagc tgctgcataa tcccggcgga tttatgttca tagttgatgg
    14761 caatcgccgg agtagcaaag ttcattgaga taatggcgga gtgcaggcgc gtaccgacgg
    14821 tgagttcaca ggctccgaga attttgccca tttccagatc gttgagttca tccatcacta
    14881 cgtggtaacg ggcaggatcg ctgatgtgct ggcgcagatt gagcgccacc atgcggtcgt
    14941 ctttgttata gctgtcgata cccgtacagg tggagagcgc aatcacctga tagccttcat
    15001 cgagaatgcg attgaccacc ccggcaaagg ctttttcata cgcttgttga gtggtgccga
    15061 gacgtttgtc gaacggtgcc agttcgcgca gggtaattgc cactgttttc tgttgtgcgg
    15121 caacgtccag ccagtgttga acggcatagc tggcggtgaa gtcttcggtg tggtgatcga
    15181 ccagccacgc ggtatcgacg ccatgttcca cttttgcggt ggtgatattg ctgcgtttca
    15241 tcaaatcaag gctgaccgat tcgcgcagga tcagcgcgtc gcagtgacca aagacatagt
    15301 tcgccagttg gttaaattgc tcatcctgga aggggccgac gctgtgacca atcataaaca
    15361 gcggcttttt cgccataaat gtgcaaagcg catgttcaaa ctgcggcacg ccgtagagat
    15421 cgacaaaaaa cgatccgccg acctgaataa tggcgtcgta acctgacagc aggcgcacga
    15481 agtcggtgaa tccctgggcg atggcgatat tgcgcagctt gccagtgtcg gtgacgcgtg
    15541 agagcaatac ctgatgctgg tagcggcgac ggaggacttt tttaacgcgc ccgacaacgc
    15601 ccgccgcgct attgtgttgt gtcatctgca ggaacagcgg atcgcccatt accgggcggt
    15661 tgagcagcca ggaagaactg accggatagc ggctcatcac gtccacttcg gtatgtggat
    15721 tgaggatgtt gatggcatca agtaagccgc gcaagatggc gctgtcgcca cgattgccgc
    15781 aagtgtggtt gcccagaata agtaatttca ttttttcctc ataaatttga tgccaggtga
    15841 ggctgtgttt gttgtgttcg ttgccgtttt ttgcctgatg cgacgctggc gcgtcttatc
    15901 aggcctacag gtcccgagca cagaaccgta ggacggataa ggcgttttac gccgcatccg
    15961 gcaaccgttg tcggaaccga aaacagcaat tcaccccgcc cgtaaaagca ttttcatttt
    16021 ttcgctgcga caaaactgac gcttcacttc caccaccagc ggatggcgtg acagcacaat
    16081 catcaccaca aacgccagca cgcccgcggc tatttgcacc gccagcagca tccccagcgc
    16141 cagttgccct ttcagcacaa tgcccagcgc ataactgacc accagcgtcg gcaacgagag
    16201 ataaaacggc agccacaaac tcaggatgta ctggcgataa ctggaaccaa gaaccggttt
    16261 aatcatcacg aagtaactca gaatggtgtt gataatctgc accagcagga aaccgagcgt
    16321 gacgccgatt gcgcccgcca tctgcccacc aataacaatc gccggaataa acagaaatgt
    16381 tttgaataca ttgaatttaa agctgatatc gacccgcgct ttcgccatca gcagcgaacc
    16441 aatcgggtta cccacggagc gcagcagacc cacaatacac agcaattgca gcaccggaat
    16501 aatgctgttc cacttttcgc cgaataccag cggtacgaag ttattcgaca ccaccatcag
    16561 ccccagcagc gccggaaagt tgataatccc cactatcgac agcagcttgt agaagttaac
    16621 acgcagcttt tcggtgtcgt cctgaatttt ggcaaacgcc ggaaacaaca cgcgggtgat
    16681 gattgggttc agcttcatcg gcggtacgac ggccacgttg tacgccaggt tgtatccccc
    16741 tgccacgccc gcgccaagaa tacgcgccag caccagcgtt gaaaggttgg tgttgagata
    16801 gttgatgatg ctgtccgccg tcagccaggc accaaaacgt aagttcggtg ccacagacgc
    16861 cagcgagaag tgcagaccgg ggcaataaat tttgcgtcca aagtagccaa acagcagtgt
    16921 tctcaccgca ctattgacca gataaccgag gatcgcggtc atcgccagcg gccagaaatg
    16981 ggcgctaact accgtaaaag tgaagcccgc cagcaccgcg ctggtttcga tcatgccgat
    17041 tttgttgaac tccagctcct tctgcatcaa cgcgcggaac tgttgcccgt gggggatcac
    17101 cacaaacgcc agcgacaatg ttttaatcaa cggtgccagg tccgggttat tcagcacgtc
    17161 gccgatgaca tcactcaaca aaaacaccgc cacacatacc acaagcccca gcccgacgtt
    17221 cagccagtac agcgtggtga gttcaaggtg actgatttct tttcgctgaa taatcgagtt
    17281 agcgataccg aagtcagaaa gcgtatctgc cagcgcgata atcaccagcg acacagtcag
    17341 caggccgaac tggtggttgt cgataatccg cgccagcacg gtcatctgca ccagcccgag
    17401 gccgatgatg atcaccgtgg caatagccga ccacttcgcg ccgctgatgg ttttttcacg
    17461 taagctcata tcaatatgcc gctttgttaa cgaagccctt gaataccgtc aggaaaacga
    17521 ttttgatatc gaaccagacg ctccattcgc ggatgtactc aaggtcgaac tcgacgcgtt
    17581 tttccatttt ctccagcgtg tcggtttcgc cgcgccagcc attaatctgc gcccagccgg
    17641 taatgcccgg tttcacttta tggcgcagca tgtagccttc aatgagctgg cgatactgtt
    17701 cgttatgcgc tactgcgtgc ggacgtggac cgacaatcga catccccccg gtcagcacat
    17761 tgataaactg cggcaattcg tccagcgagg tgcggcgcag aaagttcccc actttagtga
    17821 cgcgcggatc gttctgcgtc gcctgggtca ccactttgtc gttctccatc actttcatgg
    17881 aacggaactt ccacactttg atcggcttgc catccatacc atagcgagtc tggcggaaaa
    17941 taaccggccc tggtgaactg agtttcaccg ccagcgcaat acagcacagc accggggaga
    18001 tcagcagcag aataagcgtc gccagcacaa tgtcttccgc acgttttagc aggcggttac
    18061 cccccgaaag cggcgtgtca tacagcggca ccaccggaac gccgttcatc tcttcgagac
    18121 gtgaatggag aatgttgaag gtaaagacgt cggggatcag cagcaccgaa caggtggtat
    18181 ccgccagttg atgggccagt tttttcactc gtgcgccgtc gcacatttgc atcgcgatat
    18241 agacgttatg aatcttgccc gcttttgcat cctcgaccag ctgttgcagg ttacccgccc
    18301 agtcgttaga aacgccgccc ggtttcgggt cgtggtaaac gcccaccact tcaaacccta
    18361 accacggctg attacggaaa ctctccatca gcatttgtcc ggcggctaaa tcccccgcta
    18421 cggcgaccat ccgcttgttg tagccatgat tacgcagcca gcccgcccca atgcgaatac
    18481 atgaacggca aaccaccagt ccgatgctgg tcagcccata ccacgccagc cagattttca
    18541 gttgcgtgtc gaaatcattg ttgaacgcca ccagtccggc gctgaaaatc acgcttaagg
    18601 tccagttttg tagcaacagg gcaaattctg tcgctgcccg aacaccgcgc catgagcgat
    18661 aaaaatcggt gatgccgctc agcatctgga acaccaccag cgtaatcagc gccaccaaca
    18721 ggtgcatgta gaggaatgac agtccgctga cttcgcaaac cagccatagc ccggcaaaca
    18781 tgatggtgat atctgaaaag cgttgcacca tagagattaa cgatgcattg gttttcgctc
    18841 gctcgcgctt ttttagattt gtcatcgttg ttcctgttat tagcccctta cccgcagtgg
    18901 ggtaagggaa gatccgacat tactcgttca gcaacgtcag cagagttcgc gttcgcgctt
    18961 ccatcagcgg cacatcaccg cgcgattcca cattcaggcg caccaccggt tcggtattgg
    19021 aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg ctgcgatcca
    19081 ccgccagcgc ctcacggcta aaatgctgtt ccacgcggtt aatcgcctca acgggttgcg
    19141 ccagtttgct gttgatctca ccgcttgccg gaaacgccgc catccggtcg cgcaccagtt
    19201 cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac gggatcatgc
    19261 cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttcg ccaccgtaga
    19321 tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc gacattaccg
    19381 gcgtgccgcc tgcagcagtc accacatcaa tagtgttcca ggagagacgc ggatcatgga
    19441 tgatcttcgc gccgggattt ttttcgagga acgcttctgc cagcaggccg acaatgtagt
    19501 agccctcgat aaactgccct ttttcgtcaa acagaaaaca gcggtcaaaa tcgccatcaa
    19561 aggcaatgcc catatccgcg ccgtgtttga tgaccgcatt gcgggtgtcg tcgcgacatt
    19621 ccggcagcag cgggttagga ataccgttgg ggaaattgcc gtccggtgtg ttgtgtactt
    19681 tgattaattc cacgggcgcg ccgagggctt taaagcgggc ttcaatggcg tccaccaccg
    19741 gacccgctgc gccattcccg gagttgatca ccagcttgag cggcgtgaga ttcttgacat
    19801 tgatataacc gaacaggtga tcaacgtaag cgtcacgcag gttgatttgc tgatagcgac
    19861 cgcgtttggt ttcatcgacg ggaggaaagt cgttggcttc agccagacgc tggacgtcgc
    19921 gcagtccggt atcaccgctg atcgggcgag ccccctcgcg caccagcttc atgccgttgt
    19981 aatccatcgg attatggctg gcagtaactt caatgccgcc atccacgccg agatggaacg
    20041 tggcgaaata gatctcttcg gtgccggaca taccgatatc cagcacatcg acgcccgcat
    20101 cctgtaaacc ttttgccagc gccagtttta aggtttcgct ggtgagacgg acatcaccgc
    20161 ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg cgccaggcga
    20221 tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta aagcaggtta
    20281 attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa acggccaatt
    20341 ttatttttgt cgttatttac accgcggttt cgcattcatt gcctgatgcg acgtttacac
    20401 ccgtccgtag cgatccgcga agcgcaccac atcatcctct tcgagataag agccggagcg
    20461 cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcagtgcg tcgcccccag
    20521 cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa tggtgacttt
    20581 tgccgttccc gcgaccacta cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa
    20641 accctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg cgtcgataga
    20701 gtcatatttg ccccacggac gatacacttc gcgatgcacc cgatgctcat ggcgaccatc
    20761 ggctttgatc tgctcgacca cttttttcac atcctgtacc gcgttacggt cggcaatcag
    20821 caccgcatct ttggtctgca ccaccaccag atctttcacc ccgacggtgg tgactaggcc
    20881 tgattcggca tacacatagc tgttttcagt tttgtgatta atcacatcgc cgtggcaaac
    20941 gttgccctcg gcggtgtggg cgctgatctc ccataatgaa gaccaggaac caacatcgct
    21001 ccagcccgca tccatcggca ccacaacagc atctgccgta cgttccatga ccgcgtaatc
    21061 caccgactct tccgggcagg cgagaaacgc ttcttcatct acacgaataa aatcgagatc
    21121 cggatcgacg gcgctcatcg ctttttcgca ggcatcgagg atatccgggc gatatttttt
    21181 cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc agtaatattc
    21241 gccgcttgct acataggcct gggcggtttc cagattcggt ttttcgacaa actgcgccac
    21301 ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa tatagccata
    21361 accggtttct ggtagatccg gcacaatgcc aaaggtcacc agcttgcccg cttcggcata
    21421 aggcatggca ttacgcacgg cggcacggaa cgcgtcttta tcggcaatca catgatccgc
    21481 cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg ccagcgccgc
    21541 cagtgcaatg gcaggtgcag tgttacgccc tgccggttcg agaataatgt tctcggtgag
    21601 tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat tgcaaatcac
    21661 caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca gcatggtgag
    21721 atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg gccataaacg
    21781 gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca taattatccc
    21841 cgaatatcat ttataaattg acgtaacacg ttctctttat cgagcgtgcg ttcggcatat
    21901 tcacgtgcca ccgtgttatg tttggacagc aggagcgcct gacgaatccc cgccaccagc
    21961 gcctcgaccg attccggttc tacgcaaacc gcaatacccg gaaaggtttc gcaaagctgc
    22021 cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc cagaatattg
    22081 gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttacaccac cagatggcaa
    22141 tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcggttc atcgcgcagg
    22201 agatcggcag cttcaataac gttttccagc ccctgctttt caccaatatt gccggagtaa
    22261 agaatgattt ttttgttatc cggcaggcca agctggttac gaagggcatc aacatcggcg
    22321 tctgcaacat gctgaaaacg ggcaatttcc gaccagttgg ggaagaagat gacgttatcc
    22381 gccgccacgc ctttttcgat ggctttattc atcatcgaac gcgaaatcgt ggaaacgtta
    22441 tcgacgttat gcagtccgct acgttcgaac gccgttgcca gctgtgccac tttgccgcct
    22501 ttgccttttc cggcaaggcc cagccccagc atggcgtcca cttcgtaatc ctgaatatgc
    22561 agcacggtac gcgcaccaga gagcttcgcc agcaggcgca ttcccggcgt gcaaaacagc
    22621 gttggcacta cgccaataat gcgatccggc ttccagcgac gttgcgccat cagtggaaag
    22681 aaactgctga cggcaaaact gccgagatgc aacaagcgtt tcagggtgct cggctgtttt
    22741 ggcacgtaca gcgggcagcg ccacaccgtg gcggcccctt cttctcgttt gtagcgccag
    22801 gcggaatagt tctcacccac ctgccattgc gggtagtaag gcggtgcggt aatgacccgc
    22861 acctcatgac cttgtgccgc caaccattcc accatctcgc cggtgtattt gccgatgccg
    22921 gttaactccg gcgagtagtt aatgccgtag accagtattt tcataatccg ggtactccgg
    22981 cacgcttctc agcgagaaaa taggcgcggc tgttagcgtg aacatcgtta ctggcgagca
    23041 gcgcgtccgg cgtcagccag cggtaatcgt catgctgctc atccggcagt aacagctctt
    23101 cttccgctac tctgaagcga aaaccgagca ccacatagtg agtggtgaaa tccgtgccgg
    23161 agaagttatc gtcataaaaa tgctgccaga caccgtaaaa ctggcctgct gttatcggca
    23221 gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc gtttcgtctt
    23281 tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc cgggcggttg gttcttttgc
    23341 caagcagaaa ctcgccgcga ctgttctcga caataaagtc gagagagaca agcggtgtgg
    23401 agcgcactac agtggcaaag tcttcctgac gtaaaaacat cattcccccc gaaagcggtc
    23461 ttggttctca aggaaccact ggtaagtgct ggcaagcccc gcttccagtg agatttcgtg
    23521 ataccagcca agctgatgca ggcgcgtcac atccagcagt ttgcgcggcg taccatccgg
    23581 tttgctggca tcaaaaacca cccgaccttt gtaacccacc actttggcga tggtttgcgc
    23641 cagctcgcgg atagtgcagt caacgcccgt gccgacgtta atgtgcgaca gcatcggttg
    23701 gctattctcc agccagactt catgcgccag ctccatgaca tgaatgctcg ccgccgccat
    23761 atcatcgacg tgcaggaatt cacgcatcgg tgtaccgctg ccccatacca ccacgtccgg
    23821 cgcattctgt gccgtcgcct cgtggaagcg acgcagcaat gctggtatca catgggagtt
    23881 actcgggtgg aagttgtcat gcggcccgta caggttggtc ggcatgacag agcggtaatc
    23941 gcgtccatac tggcggttgt aagattcgca cagtttgatc ccggcgattt tggcaatggc
    24001 gtaaggctcg ttggtcggct ccagcgtgcc ctgcaataac tcgctttctg ccatcggctg
    24061 ttttgccagt ttcgggtaga tgcaggacga tccgagaaac atcagtttgt tcacgtcgtt
    24121 ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg ttctggtaga tgaaatccgc
    24181 cggataggtg ttgttggcaa caatgccgcc cactttcgcc gccgccagat agacctggtc
    24241 aatgcgttcg ctggcaaaga aatcatgcac ggcgcggctg tccagcaggt tcagttcatc
    24301 gcgggtacgt aataccagct ccacatcacc gcgctgttcg agctgccgct tgatggcaga
    24361 accgaccatc ccacaatggc cagcgataaa aatgcgttgt ttacttatgc ttatgactcc
    24421 agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag agtgtttttt cgccgcttcg
    24481 aggtcattag ccaccatttc agacaccatc tctctgaggg tgatttccgg tttccagccc
    24541 agtttttcgt gcgctttggt cgggtcgccg agcagcgttt caacttctgc cggacggaag
    24601 taacgcggat caacggcgat aatcacatca cccggtttaa cgcccggcgc gtcatgcccg
    24661 gtgacggaaa ccacaatgcc cttctcttca acacccgtgc cttcaaagcg cagtttgatg
    24721 cccagctgtg ctgccgccat ttccacaaac tgacgtacgg agtactgaac gccggtcgcg
    24781 ataacgaaat cttccggctg ttcctgttgc agcatcatcc actgcatttt tacgtagtct
    24841 ttggcgtggc cccagtcacg cagggaatcc atattgccga ggtacaggca cgactccagc
    24901 ccctgggcga tgttggcgat tgcgcgggtg attttgcggg taacgaaggt ttcgccgcgg
    24961 cgcggggatt catggttgaa gagaattccg ttacaagcgt acatgccgta ggattcgcgg
    25021 tagttaacgg tgatccagta ggcgtacagt ttggcgaccg catacggaga tcgcgggtag
    25081 aacggcgtgg tttctttctg cggaatttcc tgcaccagac catacagttc agaggtggaa
    25141 gcctgataga aacgagtttt cttttccaga ccgaggaagc ggatcgcctc cagcaggcgc
    25201 agcgtaccca tcgcgtcgac gtcagcggta tattctggtg actcaaaaga gaccgcaacg
    25261 tggctcattg cgcccaggtt gtacacttca tccggctgta cttcacgcaa aatgcgcgtc
    25321 aggttagagg tatcactcag gtcgccataa tgcagatgga atttcgggtt gcaggtgtgc
    25381 ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg acgatgcgcg acgcttaata
    25441 ccatgcacct cgtaaccttt ttccagcaga aactctgcca ggtaagaacc gtcttgtccg
    25501 gttacaccgg tgatgagagc gacttttgac atgtcttatt cctctgtatt tttgaattta
    25561 ttcagtttca acgcgttcgc gtatcaccac tgcgggattc ccccggcaaa ccacatttgc
    25621 cggaagcgat ttaaaaacac tgcttcgtgc acccacgacg gtgccgtcgc cgattgtgac
    25681 gcctggggca acaaagacat cggttgccag ccagcatttc tcgccaatca caataggcgt
    25741 ggcgttaatg gtgaaatgtt gacttgcatg gtcgtggcta ccggtgcata aataactttt
    25801 ttgcgatatc accgaatgtg cgccaatggt tatttcaccg agggtatata aattgacgtc
    25861 atcgccaacc cacgcgtaat cacctaaggt taatttccac ggataggtaa tttttactga
    25921 cggacgaata actacgtttt ttcctatttt tgctccgaat aaacgtaata aaaaagcccg
    25981 ccagcgatac aatacttgtg gcgaccaggc aaatattgtt gcctgtactg cccaccataa
    26041 ttgcacttta atagcgttgc cgccccggaa ccctttcggc accgagaaac cgcttaaatc
    26101 ttgcattggt tttccttata ttcagacttt gttatataag gctttagtct tacctgtcgt
    26161 acgttggcgt aaatgccagg ataattctgc ccagaagcca ggtacatgta atatctgccg
    26221 ttggactttt ttggcgtcag cac
//
LOCUS       DADMFL010000053        25440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.053, whole genome shotgun sequence.
ACCESSION   DADMFL010000053 DADMFL010000000
VERSION     DADMFL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.053"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            116..1087
                     /locus_tag="JNZ27_002181"
     CDS             116..1087
                     /locus_tag="JNZ27_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY8664511.1"
                     /translation="MRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1110..2621
                     /locus_tag="JNZ27_002182"
     CDS             1110..2621
                     /locus_tag="JNZ27_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8664512.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVQTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2646..3644
                     /locus_tag="JNZ27_002183"
     CDS             2646..3644
                     /locus_tag="JNZ27_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8664513.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3677..4771
                     /locus_tag="JNZ27_002184"
     CDS             3677..4771
                     /locus_tag="JNZ27_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY8664514.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4783..5655
                     /locus_tag="JNZ27_002185"
     CDS             4783..5655
                     /locus_tag="JNZ27_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY8664515.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5703..6125)
                     /locus_tag="JNZ27_002186"
     CDS             complement(5703..6125)
                     /locus_tag="JNZ27_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8664516.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6222..7424)
                     /locus_tag="JNZ27_002187"
     CDS             complement(6222..7424)
                     /locus_tag="JNZ27_002187"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY8664517.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGISISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCERWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(7434..7973)
                     /locus_tag="JNZ27_002188"
     CDS             complement(7434..7973)
                     /locus_tag="JNZ27_002188"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY8664518.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASKHAATGLIRQLAYELAPKVRVNGVGPCGMASDLR
                     GPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRRNNRALSGVMIN
                     ADAGLAIRGIRHVAAGLDL"
     gene            complement(7973..8491)
                     /locus_tag="JNZ27_002189"
     CDS             complement(7973..8491)
                     /locus_tag="JNZ27_002189"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY8664519.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(8488..9849)
                     /locus_tag="JNZ27_002190"
     CDS             complement(8488..9849)
                     /locus_tag="JNZ27_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY8664520.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASYEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            9985..10875
                     /gene="hcaR"
                     /locus_tag="JNZ27_002191"
     CDS             9985..10875
                     /gene="hcaR"
                     /locus_tag="JNZ27_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY8664521.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11035..12174
                     /locus_tag="JNZ27_002192"
     CDS             11035..12174
                     /locus_tag="JNZ27_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY8664522.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12166..13446)
                     /gene="csiE"
                     /locus_tag="JNZ27_002193"
     CDS             complement(12166..13446)
                     /gene="csiE"
                     /locus_tag="JNZ27_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY8664523.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13637..14491)
                     /locus_tag="JNZ27_002194"
     CDS             complement(13637..14491)
                     /locus_tag="JNZ27_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8664524.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(14636..15439)
                     /gene="suhB"
                     /locus_tag="JNZ27_002195"
     CDS             complement(14636..15439)
                     /gene="suhB"
                     /locus_tag="JNZ27_002195"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY8664525.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            15558..16298
                     /gene="trmJ"
                     /locus_tag="JNZ27_002196"
     CDS             15558..16298
                     /gene="trmJ"
                     /locus_tag="JNZ27_002196"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY8664526.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            16568..17056
                     /gene="iscR"
                     /locus_tag="JNZ27_002197"
     CDS             16568..17056
                     /gene="iscR"
                     /locus_tag="JNZ27_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY8664527.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17168..18382
                     /gene="iscS"
                     /locus_tag="JNZ27_002198"
     CDS             17168..18382
                     /gene="iscS"
                     /locus_tag="JNZ27_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY8664528.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            18410..18796
                     /gene="iscU"
                     /locus_tag="JNZ27_002199"
     CDS             18410..18796
                     /gene="iscU"
                     /locus_tag="JNZ27_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY8664529.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            18813..19136
                     /gene="iscA"
                     /locus_tag="JNZ27_002200"
     CDS             18813..19136
                     /gene="iscA"
                     /locus_tag="JNZ27_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY8664530.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19232..19747
                     /gene="hscB"
                     /locus_tag="JNZ27_002201"
     CDS             19232..19747
                     /gene="hscB"
                     /locus_tag="JNZ27_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY8664531.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            19764..21614
                     /gene="hscA"
                     /locus_tag="JNZ27_002202"
     CDS             19764..21614
                     /gene="hscA"
                     /locus_tag="JNZ27_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY8664532.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            21616..21951
                     /gene="fdx"
                     /locus_tag="JNZ27_002203"
     CDS             21616..21951
                     /gene="fdx"
                     /locus_tag="JNZ27_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY8664533.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            21963..22163
                     /gene="iscX"
                     /locus_tag="JNZ27_002204"
     CDS             21963..22163
                     /gene="iscX"
                     /locus_tag="JNZ27_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY8664534.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22222..23505
                     /gene="pepB"
                     /locus_tag="JNZ27_002205"
     CDS             22222..23505
                     /gene="pepB"
                     /locus_tag="JNZ27_002205"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY8664535.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            23647..24423
                     /gene="sseB"
                     /locus_tag="JNZ27_002206"
     CDS             23647..24423
                     /gene="sseB"
                     /locus_tag="JNZ27_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY8664536.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(24869..25351)
                     /gene="sseA"
                     /locus_tag="JNZ27_002207"
                     /pseudo
     CDS             complement(24869..25351)
                     /gene="sseA"
                     /locus_tag="JNZ27_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6014 a         6349 c         7068 g         6009 t
ORIGIN      
        1 ttcactaaac cctattaact gtaatatttg agcggcacgc gctgccgctc atcacaaaat
       61 aattagaata atccgttgtt aataaaaacc ctacaggagg cactatgcct aaaaaatgag
      121 aacaacccgt aatctattat taatggctac gctattaggt agcgcactat ttgccagggc
      181 tgcggataaa gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg
      241 agtgcgtcag ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga
      301 aactaacgcc cagggggata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg
      361 taatgtcgat gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg
      421 tcgcgccagt gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg
      481 tgtcgataaa tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact
      541 gggtaacgct gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt
      601 catcaattgc gaagcctttg aagtttgtgt gcaacgacgt aaaggatttg aagaagtatt
      661 aaaatcccgc gttcccggcg cgcaaattgt cgctaaccag gaagggactg ttttagataa
      721 agcgatttcc gttggtgaaa aactgattat ctccacgccg gatctcaacg ccattatggg
      781 ggagtcgggc ggtgcgacac tcggcgcggt aaaagcggta cgtaatcaaa atcaggccgg
      841 aaaaattgct gttttcggtt cggatatgac aaccgaaatt gctcaggagc tggaaaacaa
      901 tcaggtgctg aaagcggtag tggatatttc cggtaagaaa atgggcaatg ctgttttcgc
      961 gcaaacattg aaggttatca ataaacaagc cgacggtgaa aaagtgattc aggtgccaat
     1021 cgatctctat accaaaacgg aagatggtaa acagtggctg gcaacgcacg ttgatggtct
     1081 gccctaatcg ctgattcgca gaggtcccta tgttcacggc aacagaggca gtcccggtag
     1141 caaaagtggt ggcaggaaat aagcgttacc ccggcgtcgt tgcgctggat aacgttaact
     1201 tcacgctcaa taaaggcgaa gttcgtgcgc tgttaggtaa aaacggcgcg ggcaaatcga
     1261 ctctcattcg aatgcttacc ggcagcgaac gcccggatag cggtgatatc tggattggcg
     1321 agacgcgact ggaaggtgac gaagctacgt tgactcgccg tgccgctgaa ctgggggttc
     1381 gtgcggttta tcaggaatta agtctggtgg aagggctgac ggtggcagaa aacctctgcc
     1441 ttggtcaatg gcctcgccgc aacggcatga ttgattacct gcaaatggcg caggatgccc
     1501 aacgttgctt acaggcgctg ggcgttgacg ttagtcctga acaacttgtt tcaacgctaa
     1561 gcccggcgca aaagcaactg gtggaaattg cacgggtgat gaagggcgag ccgcgcgtgg
     1621 tcattcttga tgaacccacc agctcgcttg ccagtgcaga agttgaactg gtgatcagtg
     1681 cggtgaaaaa gatgtcggca ctgggcgtgg cggtgattta tgtcagccac cggatggaag
     1741 aaatccgccg cattgcctcc tgtgccaccg ttatgcgcga tggtcaggtg gcgggcgatg
     1801 tgatgctcga aaacacctcc acgcatcata ttgtgtcgct aatgctaggg cgtgatcacg
     1861 ttgatattgc gccggttgcc cctcaggaaa ttgtggatca ggccgtactg gaagtccgtg
     1921 cgttacgcca taagcccaag ctggaggata tcagctttac gctacgtcgt ggcgaagtgc
     1981 ttggcattgc tggcctgctg ggggcagggc gcagtgaatt gctgaaggcg attgttgggc
     2041 tggagacgta tgaacagggc gaaattgtta tcaacggcga gaaaatcatg cgccccgatt
     2101 acggcgacat gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gaagcgggga
     2161 tcattccctg gctgggcgtt gacgaaaata cagtgcagac caatcggcaa aaaatcagca
     2221 ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga
     2281 tgacggtcaa ggctgccagt agcgaaacac ccatcggcac gctttctggt ggcaatcagc
     2341 aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc
     2401 caacgcgcgg cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgtgagctgg
     2461 ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt ggaggaattg ccgctggtgt
     2521 gtgaccgcat cctgttatta cagcacggca cgttctcgca ggagtttcac tcgccggtca
     2581 atgtggatga gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc
     2641 tgaagatgtc tgcttcgtca ttaccattgc cgcagggcaa gagcgtctcg ctcaaacaat
     2701 ttgtcagtcg ccatattaat gagatcggtt tgctggtggt gattgccatt ctttatctgg
     2761 tcttctccct gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg
     2821 atgccgccac cattgggatt gccgcctggg cgatgacgct gattattatc tccggtgaaa
     2881 ttgatgtcag cgttgggccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc
     2941 aatttgaagt tccgttggcg attgcttgtc tgctggtgtt gctgttaggc gcgctgatgg
     3001 gaacgctcgc cggggtgctg cgcggcgtgt ttaacgtacc aagtttcgtt gccacactgg
     3061 ggctgtggag cgctctgcgc ggaatggggc tgtttatgac caacgcacag ccagtgccga
     3121 ttgacgaaaa cgaggtgctg gactggctgg gcggacaatt tctcggtgtg ccagtatccg
     3181 cgctgatcat gatggtgttg tttgcgctgt ttgtgttcat tagccgaaaa accgccttcg
     3241 ggcgctcggt ttttgccgtt ggcggtaatg ccacggcggc gcagttgtgc ggtattaacg
     3301 ttcgtcgagt gcgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca
     3361 ttttgttggc ggcacgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg
     3421 acgtcatcgc cgcggtcgtg gtcggcggta cggcactttc cggcggtcgc ggttccctgt
     3481 tcggtacatt gcttggtgtg ctggtgatta cgctaatcgg taacggtctg gtgctgctcg
     3541 gtattaactc ctttttccag caggtggtac gcggcgtcat catcgtggtg gcggtgctgg
     3601 cgaatatctt gctgacccag cgaagcagta aagcgaaacg ctaaacctta acgcccctgg
     3661 cgcgggtgcg gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat
     3721 gctggcagct tatttaccag gaaattcaac cgtcgatctg cgggaagttg cggtgccgac
     3781 gccggggatt aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt
     3841 ccactatatc tatcatcaac accgtgccac ggcggcggca cccgataaac cgttatacca
     3901 gggatttatc aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctgccg
     3961 ccattttaaa gagggcgacc gcgtgctggt gtatcacatt tctggctgtg gtttttgccc
     4021 gaactgccgc cgcggctttc ctatctcttg tactggcaaa ggaaaagcgg cttacggctg
     4081 gcagcgtgac ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct
     4141 gccggatgcg ctgagctacg aagatggtgc gtttatcagt tgcggcgttg gcacggccta
     4201 tgaagggatt ttgcgcggcg aagtttccgg cagcgataac gtgctggtgg tcggtctggg
     4261 ggcggtcggc atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaac ggatcatcgg
     4321 cgttgatatg ctgccggaac gtctggcgat ggcaaaacag ttagggggga tggatcacgg
     4381 ctatttagca accaccgaag gtctgccgca gataattgct gaacttaccc acggtggcgc
     4441 ggatgttgcg ctcgattgtt ccggtaatgc cgcaggtcgc ttgctggcac tgcaatccac
     4501 tgctgactgg gggcgggtgg tttacattgg tgaaaccgga aaagtggaat tcgaggtcag
     4561 cgctgatctg atgcaccatc aacggcggat tatcggctcc tgggtgacca gtctgttcca
     4621 tatggaaaaa tgcgcccacg atttgactga ctggaagctg tggccgcgta acgccattac
     4681 ccatcgcttc tcgctagaac aggcaggtga tgcctatgtg ctgatggcga gcggcaaatg
     4741 cgggaaagtt gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta
     4801 tcccacgggt cgctaaagct ggacgtttct gaccagggcg gcgtaatcga aggcttctgg
     4861 cgcgatacga cgccgctctt acgtcctggt aaaaaaagcg gtgtggcaac cgatgcctca
     4921 tgttttccgc tggtgccatt tgcaaaccgg gtgagcggca accgttttgt ctggcagggg
     4981 cgtgagtatc aactgcaacc gaatgttgag tgggatgcgc actatctaca cggcgatggc
     5041 tggctgggcg agtggcaatg tgtctcgcac agcgatgata gtttgtgtct ggtgtatgaa
     5101 catcgcagcg gtgtctatca ctatcgggta agtcaggcgt ttcatttaac ggcggatacg
     5161 ctgacggtga cgctctctgt caccaatcaa ggggcagaga cgctgccatt tggtaccggc
     5221 tggcatcctt attttccgtt gtcaccacaa acgcggattc aggcgcaggc gagcggttac
     5281 tggctggaac gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaggaactg
     5341 gattttaacc agctcgcgcc gttgccgcgt cagtgggtga acaatggttt tgccggatgg
     5401 aatggtcagg cccgcatcga gcagccgcag gaaggttatg ccatcatcat ggaaacgacg
     5461 ccacctgcac cgtgttattt catctttgtt tccgaccctg cgtttgataa aggatatgcg
     5521 tttgatttct tctgtctgga accgatgagc catgcgccgg atgatcatca tcgcccggaa
     5581 gggggtgatc tcattgcgct agcaccaggg gaatcaacaa cctcagagat gtcgttgcgg
     5641 gtagagtggc tgtaatttat aaaatgccgg ataaggcgtt cacgccgcat ccggcatctt
     5701 tcctaacgcc gatcgagaga aattgccccc ggtccggtaa ttgccaatag caagaacgcg
     5761 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
     5821 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
     5881 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
     5941 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
     6001 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
     6061 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acgtagtgtg
     6121 ttcataacca atccctttgc aagataatgt gatttaaacg taatacacac tcttgcacct
     6181 cgccacgccc caggcattga gatattcgct aattatcctg gttacagtga tttaagcgcg
     6241 atgttctcat ctatcagcag tttcgcatca aacgttttgc cgctctggat ccatttgcga
     6301 attgggcgaa tctcacgccc ctgattcagc gtgaccgcac cgataagcac gccgttttgc
     6361 agattaaacc aaatcgcctt ctgagtttcc gggttgccac gacaaagcca gtcatcgcca
     6421 cgcatatcgc caataaactg taagttatca ctgtactgat cgctccagaa ccacggcggc
     6481 ggcagtagcg gtagcggtag ccctaacatt gcggcagcgg caatttgcgc ctggttattg
     6541 gcgttttccc agcgttcgca gcggtgtagt gcaccattat caagacgagt gattgccaca
     6601 tcgccaccgg caaagatcgc gggatcgcag gtgcggcaag cctcatcaat gacaatgcca
     6661 ttggcagtat caaggttggc ctcgcgagcc agttgctcgt tggcgctgat actaataccg
     6721 taaatcacca catcagcctg aagcgtttcc ccactttgca gcgtcagttc tactttttca
     6781 ccatcgacca catgttcaat ggcattattg agcagaatgc gcacaccagc ctgctgatgg
     6841 cgctgtaaaa gatagcgttg cacgggcggt ggtgcattac ggcccatgac ggttgccgcc
     6901 agttcaatca ctgtcacctt acatctgcgc tgcgtggcgc tggcagccag ttccagacca
     6961 atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc tcgcagtctg
     7021 gcggcatcac cggcatggtg tagggtaaag cagcgttctc ccagtgcatc aagcaacggc
     7081 agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca gctttcgccg
     7141 ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac accggaatgc
     7201 agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa
     7261 tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg
     7321 gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc agccattgcc
     7381 gccgcttgcc cgccaccgac aataatgatc gttttttctt tcatcgtgct tccttaaaga
     7441 tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc
     7501 atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata cggccccgta
     7561 aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc
     7621 agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg
     7681 gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc ataagccagt
     7741 tggcgaataa gtccggttgc ggcatgttta ctggcggcgt gtagcgggca ctccaccgtg
     7801 gcgtcatctt ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta
     7861 atggcataaa actcgccgcc cacgttgaac aaggcgatga cgggcgaggt atctatccgg
     7921 agagcctcac cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca
     7981 gtacactcag gttatgggaa gtgatcaccg cttgatccag gacgatatcc cgttccagca
     8041 agcgccagtt gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg
     8101 tttcatcgcg ctctttttgt gcccgataaa gtagataatt caccctcacg gcaaatacgt
     8161 ttgggatgtc ggtttcgctt acctggcagt tgctgattaa gtgacgagtg cgtgacggcg
     8221 gctcttctgc ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt
     8281 tggtgtcatt gaaaatccag gtcgtcggcg gctggacgcc tttgcgacgg tcgcgtgttt
     8341 gcgcgttaac cgtggtgcgc atggtgtaac gaatctcttc gtcgagctgt gccaaccagt
     8401 cacgaaattt ccagtcatcc agcagactgg cttcgtgaaa gagaaactgg ctaatgcggt
     8461 gatgtaactc cagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt
     8521 tttatcgagc acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat
     8581 tccacgagcg gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg
     8641 gcgcttttcc tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc
     8701 tttaagcaat ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc
     8761 aggaccaaaa gcacgagtgg cgctgttttc aaaagcggct ttaacttcat aggaggcggc
     8821 tttgtcagta atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac
     8881 gcggagtgtg gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc
     8941 caggcgcagg gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta
     9001 atagcttgaa actgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa
     9061 accgctaccg tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg
     9121 gcgggcggtt tgtccatcac cgaggcgctt atcgctgcca tcatctttcg cccctaatac
     9181 ctgaacggca gaagcatggc tgaacagagc atgatactgg tcactggcga actgctctgc
     9241 cgggaatttc cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc
     9301 gccttcgcga cgatccagca tgccatccag ataccaggca atgtcaccca ggtaatcatg
     9361 caggcccggt gcgctggtat cccagttgcc aaaaatcagt cctttataac tctccacaca
     9421 aggaacttcg tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc
     9481 cagcggtaca tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt
     9541 aaaggcgcga gtgttgccac aatctgcata actcacacgc atggcccggt ggcggcattg
     9601 gttaagaaac gccttgatgc tgccgtcttt ctggcgcacc acgacaactg catcttctcc
     9661 catgtaggtg ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag
     9721 ccagcaacga ccgaaaatac gctcaagctc cagttggtaa atgtccgggt cggtataaat
     9781 acgcggagtg acccgaccgt tttgggtgtc aatcagttgg taaatgttca aatctgaggg
     9841 tgtggtcata ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat
     9901 aaggatgttg ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt
     9961 tttttcgata acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg
    10021 gcgcaggcac tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta
    10081 agtagccaga tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag
    10141 cgcaaagtcg cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc
    10201 gaacaagcgg aaaatgccaa attacgggcg cggaaaattg ttcaggaaga cagacaatta
    10261 accattggct ttgtgccatc ggcggaagtg aatttattgc caaaagtatt accgatgttt
    10321 cgtctcagac agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag
    10381 gaaaaaattc gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg
    10441 gagattgatt atctggagct ttttgacgaa ccattagtgg tggtgttacc ggttgatcac
    10501 cctttagcac atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt
    10561 accgatccgg cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa
    10621 aacagccagc caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg
    10681 ggcatggggc tgggcgtcac tttgataccc ggttatatga ataattttaa taccgggcag
    10741 gttgtttttc gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag
    10801 aaaggggaga tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca
    10861 agcgtaacgg cataattacg cattattttt gcaacatgcg ccgcggatcg cagtttttta
    10921 gtgcttgtga actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa
    10981 cttgcaccaa cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt
    11041 ttgcaatcca cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt
    11101 tttttacctt tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc
    11161 ctgttattgg gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc
    11221 gtcagcgatc cttcccgcct gatttccgcc ttgcgcgtgc tggcactgct gccacttctc
    11281 tttgctgtcg ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc
    11341 tttaacctct ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa
    11401 aagcagttcc cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt
    11461 ggctcggcgc tgacgggcaa actggtcact atgtttgatt atcgggtgat cctcgtgctg
    11521 ttgacgttgg gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca
    11581 caaggggcaa gccgccagca ggagagcacc ggttggtctg cgtggttggc gctggttcgc
    11641 cagaactggc gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac
    11701 ggttttagcg ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg
    11761 tggtcgctgg gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc
    11821 cgttgtagtg ctcgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc
    11881 attatgggag caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc
    11941 accttcacgg tctgccacct ggccgccatg cgttatattg ctgctcgcca ggttagcgaa
    12001 gtcatccgtt tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat cgctatcatg
    12061 accgttttcg ccggtttcct gtatcaatat ctgggccacg gtgtgttctg ggtaatggcg
    12121 ctggtggcgc ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag
    12181 cattttgcgg atctgctcct gttgatgtgt tgtcagcgtc aggtccgtat agatcagcgg
    12241 tggtgaaaag agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccacgcgg
    12301 agcgcctgtc tgcaaaaatg cctttaccga catatgctta atgttgagcg gcagtaacgt
    12361 tagttcgcgt aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtcagtag
    12421 aataatctgt ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc
    12481 gaccagacca ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag
    12541 cgccgcgcgg gtggtgcgta ccaggcgtgg atacagtctg gcgaactctt ccggcagggt
    12601 attatcaata ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt ggatataaag
    12661 ctgatcacat aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt
    12721 taccagacgt ttgatagact gacgcaattg tctgtcccgc tgatgcgcat cccgcagtgg
    12781 atcgggaacc cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac
    12841 atgctggagc gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag
    12901 gcaggattcc gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg
    12961 ctgcagcaaa caatattgca gatacagaca caggaaatga atatcgcgcg attcaaagcg
    13021 tttttgcagc cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa
    13081 gttacgcgca attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt
    13141 aataaatgac ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg
    13201 gttaagcact gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg
    13261 atgatagcgc aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc
    13321 cacaccatta agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt
    13381 cagcaagatc tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg
    13441 catcatctgt agatctccca taagcataat tgataagaat agctaaagtt tgctcaggga
    13501 aatggcgaag ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat
    13561 tttaaactgt tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg
    13621 tgctagcgat catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct
    13681 gttcgcgata aacattgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga
    13741 ttaggatcaa ccgttttggc tcttttgcca ggctatacaa cttttcgctg tgctgccatg
    13801 ggataacgtg atcggcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga
    13861 tgtaattttc gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg atcatctggt
    13921 tggcgatggt tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac
    13981 gatcaccctg accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca
    14041 ccagccgttg tgggtttaca tcactgcgat ggcgcaccac attgatggcg ctttgcgtat
    14101 cgtccagcaa tccggcctgg gacggagtgc cttttgattt accaaacccg cgataatcaa
    14161 acataaaaac gttgaaatta cgctcaggta accaactgac cagcggccag tgggcggaca
    14221 tatttccggc attgccgtga gcatgaatga tggttgcgat ggcgttgtca gcagggctcg
    14281 tcgaagaagg aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg
    14341 attccgccga ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga
    14401 tggcaacgcg cagcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt
    14461 tgggaacgcg tttgttcact ggcagtgcca taacctttcc ttgcgacttc tttatcatcc
    14521 attaatcggg gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt
    14581 tcggaatagc gcctgaaagg cgagggcggg tgagtgatat cacccgcctg agtcattaac
    14641 gcttcagagc gtcgcttaac tcgtcacgca tgttcgccag tatggctttc acaacacgcg
    14701 ggttaccagc aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga
    14761 cgatgccgcc cgcttcacga accagcagct cgcctgccgc gaaatcccac ggacgcagac
    14821 cgatttcaaa gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag
    14881 aaccggtacg acggaagtct gcacattcgt tgaacagctt gccgacgatg ttgatgtagg
    14941 tagtggcgta ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat
    15001 cgcgagcggt gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg
    15061 cggtgaacag ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga
    15121 tacgaacggc gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat
    15181 ccagtggatc gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt
    15241 cggtgatgat ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcgg
    15301 cagctttatc tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt
    15361 ccggggtttc atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga
    15421 tgttcagcat cggatgcatc ggtctctctc actggatgtt aaagaacggg aaaacggcgc
    15481 agagtatagc agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct
    15541 ttagatgaag aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca
    15601 ccggcaatat gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc
    15661 tggttaatcc actggtgaaa cccgactccc aggcgattgc cctggctgca ggggccagcg
    15721 atgtgattgg taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagcc
    15781 tggtggtggg caccagcgca cgctcccgca cgctgccgtg gccgatgctc gacccgcgcg
    15841 aatgcggtct gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc
    15901 gtgagcgcgt cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg
    15961 cggcaaaccc ggaatacagt tcgctgaacc tggcaatggc ggttcaggtt attgcctatg
    16021 aagtgcgtat ggcctggctg gcaactcagg aaaatggcga gcaggtcgag catgaagaga
    16081 cgccgtatcc gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc
    16141 tggcaaccgg ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc
    16201 tgtttacccg tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt
    16261 ctattgagca gcagaataaa ggtaacaagg ccgaataaca gtggttgcct gatgcgacgc
    16321 ttaacgcgtc ttatcaggcc tacaatgtac agaatcgtag gtcggataag gtgttcacac
    16381 cgcatccgac aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc
    16441 gactaaatca gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg
    16501 ctatgcaata cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa
    16561 gtaagacatg agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc
    16621 gctcaactct gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggaatttc
    16681 cctttcttat ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt
    16741 acgtggacca ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga
    16801 agtgattagc gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg
    16861 ccagggcggc gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac
    16921 cggttttctc aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt
    16981 gtctggtcgt cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt
    17041 taagttacgc gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa
    17101 acacggtcgt acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta
    17161 tagagcaatg aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg
    17221 tgttgccgag aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc
    17281 tcgttctcac cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat
    17341 tgccgatctg gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc
    17401 tgacaacctg gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat
    17461 caccagcaaa accgaacaca aagcggtact ggatacttgc cgtcagctgg agcgcgaagg
    17521 tttcgaagtc acctacctgg caccgcagcg taacggtatt atcgacctga aagaactcga
    17581 agcggcgatg cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg
    17641 cgtggtgcag gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca
    17701 cgttgatgca acccagagcg tgggcaaact gccaatcgac ctgagccagt tgaaagttga
    17761 cctgatgtct ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt
    17821 gcgtcgtaaa ccgcgtgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg
    17881 tatgcgttcc ggcactctgc ctgttcacca gatcgtcgga atgggcgaag cctatcgcat
    17941 cgcaaaagaa gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg
    18001 gaacggcatc aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc
    18061 gaacattctc aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa
    18121 agacctcgca gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt
    18181 gctgcgcgcg ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg
    18241 tcgttttact actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg
    18301 tcgtctgcgt gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag
    18361 catcgaatgg gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag
    18421 cgaaaaagtt atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg ataacaacga
    18481 cgagaacgtc ggcagcggca tggtgggtgc accggcctgt ggcgacgtaa tgaagttgca
    18541 gattaaagtc aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg
    18601 ttccgctatc gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgacga
    18661 agcgcaggcg atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat
    18721 tcactgttct attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa
    18781 acgtgaagca aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag
    18841 cagcgcgagt aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg
    18901 tgagaacctc cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc
    18961 cggaagacat cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc
    19021 aatttctgga cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat
    19081 tcaccaaccc gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg
    19141 cataccgata accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt
    19201 tatcgcggcg gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc
    19261 tgcccgttat caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca
    19321 gtatcatcct gataaattcg ccagcggaag cctggcggaa caactcgccg ccgtacagca
    19381 atctgcaacc attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata
    19441 tttgctttct ttacacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc
    19501 gttcctgatg gaacagttgg agctgcgcga agagctggac gagatcgaac aggcgaaaga
    19561 tgaagcgcgg ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca
    19621 gttgatggtt gaacagttag acaacgaggc gtgggacgcg gcggcggata cggtgcgtaa
    19681 gctgcgtttt ctcgataaac tgcggagcag tgccgaacaa ctcgaagaaa aactgctcga
    19741 tttttaattt ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg
    19801 ctgcgccgca tcagcgtcgt ctggcggctg gtattgacct gggcacaacc aactcgctgg
    19861 tggcgacagt gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc
    19921 tgccatctgt tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta
    19981 acgcagcgct tgacaccgcc aacaccatta gttctgttaa acgcctgatg gggcgctcgc
    20041 tggctgatat ccagcaacgc tatccgcatc tgccttatca attccaggcc agcgaaaacg
    20101 gcctgccgat gattgaaacg gcggcggggc tactgaaccc ggtgcgcgtt tctgcggaca
    20161 tcctcaaagc actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtag
    20221 ttatcaccgt tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc
    20281 gtctggcggg gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct
    20341 acgggctgga ttccggtcag gaaggcgtga tcgctgttta tgacctcggt ggcgggacgt
    20401 ttgatatttc cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg
    20461 attccgcgct cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg
    20521 cggacattcc tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg
    20581 cagccaaaat cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg
    20641 gcgaaatcag ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac
    20701 tggcttgtcg tcgcgcgctg aaagacgcgg gtgtagaagc tgacgaagtg ctggaagtgg
    20761 tgatggtggg cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg
    20821 gtcgtccacc gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc
    20881 aggcggatat tctggtgggt aacaagccag acagcgaaat gctgctgctt gatgtgatcc
    20941 cactgtcgct gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata
    21001 ccactattcc ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga
    21061 tgtctatcca tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc
    21121 gttttgcgct gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt
    21181 tccaggtcga tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg
    21241 aggcgtctat tcaggtcaaa ccgtcttacg gtctgaccga tagcgaaatc gcttcgatga
    21301 tcaaagactc aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa
    21361 aagtagaagc ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc
    21421 tgttaagcgc cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg
    21481 cgcagggcga tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc
    21541 aggatttcgc cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg
    21601 tggacgaggt ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat
    21661 ggcgctgttc tggaagctaa tagcggtgaa accattctcg acgcagcgct gcgtaacggt
    21721 atcgagattg aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt
    21781 cgtgaaggtt ttgactcact gccggaaagc tcagagcagg aagacgacat gctggacaaa
    21841 gcctggggac tggagccgga aagccgttta agctgccagg cgcgcgtcac cgacgaagat
    21901 ttagtggtcg aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta
    21961 gtatgggact taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc
    22021 ccgatcttga tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg
    22081 aagatttcga cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag
    22141 tctggctgga cgaagccgag tgaatgaacg ggctgcctgg gggtagcccg ttttgctgtt
    22201 aaacgaataa ggataactaa aatgacagaa gcgatgaaga ttaccctctc tacccaacct
    22261 gccgacgcgc gctggggaga aaaagcaact tacagcatta ataatgacgg cattaccctg
    22321 catttgaacg gggcagacga tctggggctg atccagcgtg cggcccgcaa gattgacggt
    22381 ctgggcatca agcatgttca gttaagcggt gaaggctggg atgcggatcg ctgctgggca
    22441 ttctggcaag gttataaagg cccgaaaggc acgcgtaaag tggagtggcc ggatctggac
    22501 gatgcccagc gccaggaact ggataaccgc ctgatgatca tcgactgggt gcgtgacacc
    22561 atcaacgcac cggcagaaga attgggacca tcgcaactgg cacagcgtgc tgttgatctg
    22621 atcagcaacg tcgcgggcga tcgtgtgact tatcggatca ccaaaggcga agatctgcgt
    22681 gagcaaggtt atatggggct gcacactgtc gggcgcggtt cagaacgttc tccggtattg
    22741 ctggcgctgg attacaaccc aactggcgat aaagaagcac cggtgtacgc gtgcctggta
    22801 ggtaaaggta tcacttttga ctccggcggc tacagcatca aacagactgc gtttatggac
    22861 tcgatgaagt cggacatggg cggcgcggca acggttaccg gggcgctggc atttgccatt
    22921 acgcgcggac tgaacaagcg cgtgaagctg ttcctctgct gtgcggataa cctgattagc
    22981 ggcaatgcgt tcaagctggg cgatatcatc acctatcgca acggtaaaaa agttgaagtg
    23041 atgaacactg atgccgaagg gcgtctggtg cttgccgatg gtctgattga tgccagtgcg
    23101 cagaaaccgg aaatgatcat tgatgcggcg accctcaccg gggcggcgaa aactgcgctg
    23161 ggtaatgatt atcacgcgct gttcagtttt gacgatgcgc ttgccggtcg tttactggcg
    23221 agtgcctcac aagagaacga accattctgg cgtctgccgc tggcggaatt ccaccgcagc
    23281 cagctgccgt ctaactttgc cgaactgaac aataccggaa gcgcggcgta tccggcaggc
    23341 gcgagcacgg cggcgggctt cctgtcgcac tttgttgaga actatcagca aggctggctg
    23401 catatcgact gctcggcgac ttaccgtaaa gcgccggttg aacagtggtc tgcgggcgct
    23461 acgggacttg gtgtgcgcac gatagctaat ctgttaacgg cgtaaactaa tatgccggat
    23521 gcggcgtaaa cgccttatcc ggcctacaaa ttctgcaaat tcaacaaatt gcgaatccct
    23581 tgtaggcctg ataagcgtag cgcatcaggc atttgcgccc cgaacgttga atgaattgtg
    23641 gttcttatgt ccgaaacaaa aaacgaactt gaagacctgc tggaaaaagc agcaaccgaa
    23701 ccggcgcacc gcccggcctt tttccgtact ctactggaat ccaccgtctg ggtgcctggt
    23761 acggcggcgc agggcgaggc tgtggttgaa gatagtgcgc ttgatttaca gcactgggaa
    23821 aaagaagacg gcaccagcgt cattcctttt ttcacctcgt tagaagcgct tcaacaggcg
    23881 gttgaagacg aacaggcatt tgtcgtaatg cccgttcgca cattgtttga gatgaccctt
    23941 ggcgaaacgc tcttccttaa tgccaaactg ccgaccggta aagaatttat gccgcgtgaa
    24001 atcagtttgt tgattggtga agagggaaat ccgctgagca gccaggaaat cctggaaggc
    24061 ggtgaatcgc tgatattatc ggaagtcgca gagccgccag cacaaatgat tgattcactc
    24121 accaccttat ttaaaaccat taagccggtg aagcgtgctt ttatttgttc aattaaagag
    24181 aacgaagagg cacagcctaa tttacttatt ggcattgaag ccgatggtga tatcgaagaa
    24241 attattcagg cgacgggaag tgtagcgacc gatacattac ctggcgatga accaatcgat
    24301 atttgtcagg tgaaaaaagg ggaaaaagga attagccact ttattaccga acatattgcg
    24361 ccattctatg aacgtcgctg gggtggtttt ttgcgtgact ttaaacaaaa ccggataatc
    24421 taaaatatgc cgccctaaag ggcggcattt gggcttactt gctgctctga aggttgttaa
    24481 ccaccagtaa attgtcgccg gagagactcc ggttgttatt tacttgggaa aacagcgcac
    24541 cagaaacaat aagtacgata cagaagcacc actggcgata ttgccgcgat agcaagcgct
    24601 gagacgcgct tgctctaacg gtcgcggaac atcctttcct taagccagcg cccgcttgcg
    24661 agacggcgga aagggccgca gtggataata cactaacaga acaataataa ctctcttttg
    24721 cttgacaaaa agagagttac tggcgagtat tgtctccgct gagacgcgct tgcattacta
    24781 cggtaataca acctgctgga aaggccgcga accagaccag caataaaaaa acgccaaatt
    24841 cggcggtttt ttattgcctg taaaatactt atttcactgg ctcaaccggt aaatctgccc
    24901 gcgcgcccca ttcactccat gcgccgtcgt acagtttcac gtttggcaca tccagcgtcg
    24961 cgagtgctaa caaaaccacg gctgccgtta caccagagcc gcagctgacg ataatcggtt
    25021 tgtcatagct gacgccgcga ccaaaaaata tcgcatccag ttcatcggtc gtttttagtt
    25081 cgccttcgcg caccagttcc gtccacggaa cgttcaatgc accgggaata tgtccgcgac
    25141 gtaaacctgg gcgaggttca tcaacttctg cgttaaaacg tgcagccggg cgggcatcaa
    25201 taatttgcgc cgtattttca tggcttgcca ataacacatc ggttactttc accactgctt
    25261 caggattaaa cgcggcgtta aactctcctt ccggcagctc tactgcacct tcttctaata
    25321 gcaggtcatc gcgctgccag cctgcaagtc cccgttactt tattcgtacc ccttataatg
    25381 gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg atccgggggg
//
LOCUS       DADMFL010000054        25436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.054, whole genome shotgun sequence.
ACCESSION   DADMFL010000054 DADMFL010000000
VERSION     DADMFL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.054"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            64..522
                     /locus_tag="JNZ27_002208"
     CDS             64..522
                     /locus_tag="JNZ27_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8664537.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(532..1959)
                     /locus_tag="JNZ27_002209"
     CDS             complement(532..1959)
                     /locus_tag="JNZ27_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY8664538.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSYPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1956..2663)
                     /locus_tag="JNZ27_002210"
     CDS             complement(1956..2663)
                     /locus_tag="JNZ27_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8664539.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2821..3804
                     /locus_tag="JNZ27_002211"
     CDS             2821..3804
                     /locus_tag="JNZ27_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY8664540.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3874..4356)
                     /locus_tag="JNZ27_002212"
     CDS             complement(3874..4356)
                     /locus_tag="JNZ27_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY8664541.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4591..7173
                     /gene="leuS"
                     /locus_tag="JNZ27_002213"
     CDS             4591..7173
                     /gene="leuS"
                     /locus_tag="JNZ27_002213"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY8664542.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7188..7769
                     /gene="lptE"
                     /locus_tag="JNZ27_002214"
     CDS             7188..7769
                     /gene="lptE"
                     /locus_tag="JNZ27_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY8664543.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7769..8800
                     /gene="holA"
                     /locus_tag="JNZ27_002215"
     CDS             7769..8800
                     /gene="holA"
                     /locus_tag="JNZ27_002215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY8664544.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8802..9443
                     /gene="nadD"
                     /locus_tag="JNZ27_002216"
     CDS             8802..9443
                     /gene="nadD"
                     /locus_tag="JNZ27_002216"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY8664545.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9467..10078
                     /locus_tag="JNZ27_002217"
     CDS             9467..10078
                     /locus_tag="JNZ27_002217"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY8664546.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10338..10655
                     /gene="rsfS"
                     /locus_tag="JNZ27_002218"
                     /gene_synonym="rsfA"
     CDS             10338..10655
                     /gene="rsfS"
                     /locus_tag="JNZ27_002218"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY8664547.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10659..11126
                     /gene="rlmH"
                     /locus_tag="JNZ27_002219"
     CDS             10659..11126
                     /gene="rlmH"
                     /locus_tag="JNZ27_002219"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY8664548.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11157..13058
                     /gene="mrdA"
                     /locus_tag="JNZ27_002220"
     CDS             11157..13058
                     /gene="mrdA"
                     /locus_tag="JNZ27_002220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY8664549.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13061..14173
                     /gene="mrdB"
                     /locus_tag="JNZ27_002221"
                     /gene_synonym="rodA"
     CDS             13061..14173
                     /gene="mrdB"
                     /locus_tag="JNZ27_002221"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY8664550.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14184..15272
                     /gene="rlpA"
                     /locus_tag="JNZ27_002222"
     CDS             14184..15272
                     /gene="rlpA"
                     /locus_tag="JNZ27_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY8664551.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15411..16622
                     /gene="dacA"
                     /locus_tag="JNZ27_002223"
     CDS             15411..16622
                     /gene="dacA"
                     /locus_tag="JNZ27_002223"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY8664552.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELYAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16733..16996
                     /locus_tag="JNZ27_002224"
     CDS             16733..16996
                     /locus_tag="JNZ27_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY8664553.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17097..17738
                     /gene="lipB"
                     /locus_tag="JNZ27_002225"
     CDS             17097..17738
                     /gene="lipB"
                     /locus_tag="JNZ27_002225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY8664554.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17997..18947
                     /locus_tag="JNZ27_002226"
                     /pseudo
     CDS             17997..18947
                     /locus_tag="JNZ27_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            19148..20113
                     /gene="lipA"
                     /locus_tag="JNZ27_002227"
     CDS             19148..20113
                     /gene="lipA"
                     /locus_tag="JNZ27_002227"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY8664555.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20214..20417)
                     /gene="tatE"
                     /locus_tag="JNZ27_002228"
     CDS             complement(20214..20417)
                     /gene="tatE"
                     /locus_tag="JNZ27_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY8664556.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20546..21334)
                     /locus_tag="JNZ27_002229"
     CDS             complement(20546..21334)
                     /locus_tag="JNZ27_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY8664557.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21427..21810
                     /gene="crcB"
                     /locus_tag="JNZ27_002230"
     CDS             21427..21810
                     /gene="crcB"
                     /locus_tag="JNZ27_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY8664558.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21864..22073)
                     /gene="cspE"
                     /locus_tag="JNZ27_002231"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21864..22073)
                     /gene="cspE"
                     /locus_tag="JNZ27_002231"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8664559.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22248..22808)
                     /locus_tag="JNZ27_002232"
     CDS             complement(22248..22808)
                     /locus_tag="JNZ27_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY8664560.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23397..24782
                     /gene="dcuC"
                     /locus_tag="JNZ27_002233"
     CDS             23397..24782
                     /gene="dcuC"
                     /locus_tag="JNZ27_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8664561.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24823..25164)
                     /locus_tag="JNZ27_002234"
                     /pseudo
     CDS             complement(24823..25164)
                     /locus_tag="JNZ27_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6516 a         6317 c         6243 g         6360 t
ORIGIN      
        1 tttatagttg cagcaattac ctgtattagc caacccaaga aaacgacgtt gcgtgataaa
       61 gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc gttaaccacg
      121 gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa
      181 ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat
      241 gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg
      301 aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct
      361 gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt
      421 attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac
      481 aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa
      541 gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat
      601 gatgcccaga cagcccagcc cgccatcgtc aacgggcatt ggctgcggtt gcggctccgg
      661 ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa
      721 tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc
      781 ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg
      841 atgttctgta tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc
      901 aaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt ataaatgaaa
      961 acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat cgttaaccag
     1021 atcacgcgct tcaatcatca tcaccactaa tgggtaataa tcgacccgat gctgacggca
     1081 ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtaacaaag
     1141 ttctgccagc aggctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg
     1201 cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg
     1261 gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc
     1321 ggggataatc actgcgcgat gttgcgctaa atacaatgtc agccattctg gatacgatga
     1381 ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag
     1441 atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc
     1501 cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc
     1561 taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga
     1621 ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg
     1681 ccaggcggag ggcagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag
     1741 aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag caggcgactg
     1801 cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt
     1861 ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt
     1921 cgtttcctct atatcgagga tcttccagca atttttcata agtcgctcat atccttaagc
     1981 gtttccgggc atgtggggta gtcagccagt ggttgccagg acaaggtagg gtaatacgca
     2041 tcatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc
     2101 gctaagaatt cgcgtaataa aggcagatga tcatcttcgg gagactgcca atattgccga
     2161 ccaaaggtat aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga
     2221 tgaatacgcg acaggatcca tagactttct tcttcactca accagttatt aagtacaccc
     2281 attcgactta aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag
     2341 cattcggctg tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca
     2401 agatagtcac gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac
     2461 cgagcggcat gaccgttctc gatcattcgg tgaattgtgc gcagcaggtc atcacggtca
     2521 tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat
     2581 gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat
     2641 attttttgcg accccatgtc catataattt atccttaaac attcatttaa tgaatgcaaa
     2701 ctacatttcg agactcagtt atttatactt ttctgacgca acataatacg cataaaataa
     2761 ggcttacgca aaaatggaac acataaaaat ataatatatt ttatctacaa ccataaggaa
     2821 atgataataa tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa
     2881 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
     2941 agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag
     3001 caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt
     3061 cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact
     3121 ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa
     3181 gcggttacct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
     3241 ggatggatgt acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg
     3301 tacaaacaag ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat
     3361 aaagacggag aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
     3421 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
     3481 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
     3541 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
     3601 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
     3661 tggtataacc tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
     3721 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
     3781 cgtgagactg aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc
     3841 atattctctt tattgctatg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
     3901 ctggtcataa ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
     3961 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
     4021 cccatgatga ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat
     4081 gtctgccagc tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc
     4141 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg
     4201 cgtcagctca tcgatctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
     4261 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc
     4321 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
     4381 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
     4441 aattaatgta cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
     4501 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
     4561 gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
     4621 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     4681 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
     4741 ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc
     4801 aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     4861 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     4921 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     4981 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     5041 aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
     5101 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
     5161 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
     5221 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
     5281 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     5341 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     5401 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     5461 gccgaagctg aaatggcgac gctggagaaa aaaggcgtcg atactggctt taaagctgtt
     5521 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     5581 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
     5641 aaatacggcc tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt
     5701 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
     5761 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     5821 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg
     5881 ccgattccga tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg
     5941 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
     6001 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
     6061 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
     6121 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
     6181 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
     6241 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
     6301 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     6361 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     6421 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
     6481 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     6541 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
     6601 aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac
     6661 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct
     6721 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     6781 gagctgatga acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg
     6841 caggaagcac tgctggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc
     6901 acgctgtggc aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     6961 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     7021 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     7081 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
     7141 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
     7201 caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
     7261 acaccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
     7321 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
     7381 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
     7441 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
     7501 atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt
     7561 cattcttcga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
     7621 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
     7681 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
     7741 cacgcgtctc caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
     7801 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
     7861 gcaggaaagc caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca
     7921 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
     7981 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
     8041 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat
     8101 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
     8161 gaatcgcagc gtgcaggtga cctgtcagac accagagcag gctcagcttc cccgctgggt
     8221 tgctgtgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg
     8281 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
     8341 ctggccagac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
     8401 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
     8461 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
     8521 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
     8581 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
     8641 tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
     8701 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
     8761 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
     8821 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
     8881 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
     8941 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
     9001 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcac
     9061 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
     9121 gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
     9181 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
     9241 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
     9301 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
     9361 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta
     9421 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
     9481 aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac
     9541 acccctgacc gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt
     9601 ttcctttgac ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
     9661 tctcagtgac cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg
     9721 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
     9781 gtggtgcaat gactggcagc atgctatccc cacgaacggt gaaggatttc aggcattttc
     9841 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt
     9901 aatcgtcagc catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc
     9961 cgagtctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca
    10021 aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact
    10081 tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt
    10141 ctccgcagcc agactttttc cgccagacac gactttgtag aaattgtttt acaaaaatgg
    10201 cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac
    10261 aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat
    10321 tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg
    10381 atgacctcaa aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg
    10441 actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg
    10501 ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg
    10561 ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc
    10621 gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg
    10681 tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc
    10741 cgaaagatat gcccttcgag ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg
    10801 acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc
    10861 gcattgtcat cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc
    10921 tggaacgctg gaagctggat ggtcgcgacg taagtctact gattggcggg cctgaagggt
    10981 tgtcgcctgc ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg cttaccctcc
    11041 cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca
    11101 ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga
    11161 aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc
    11221 tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc
    11281 tgcaaattgt tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg
    11341 tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggccctca
    11401 accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc
    11461 tggacgcttt gcgcagcgtg gtagatctga ccgatgacga tattgctgca ttccgaaaag
    11521 agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa aactaacctg accgaagtac
    11581 aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata
    11641 aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggatat gtgtcgaaaa
    11701 tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa
    11761 cgcatgatat tggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga
    11821 ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag
    11881 taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata
    11941 ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt taccgatccg cgtacaggtg
    12001 gggtgctggc gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct
    12061 ccagcaaaga ttattccgcc ttgctgaacg acccgaatac accgctggtg aaccgcgcca
    12121 cacagggggt ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattaa
    12181 gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag
    12241 ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa
    12301 gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg
    12361 atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg
    12421 cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac
    12481 cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc
    12541 caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc
    12601 atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacaa ccgcatgaac
    12661 cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg
    12721 ttgctaaccg ccctaatggt acggcgcata aatactttgc cagcgcaccg tacaaaattg
    12781 cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc
    12841 acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca
    12901 atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta
    12961 cgctgatgcg ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg
    13021 cggaaaatcc agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa
    13081 aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt
    13141 gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
    13201 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc
    13261 tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt
    13321 ggcggtagat gctttcggag ccatctctaa aggcgctcaa cgctggctgg acctcggtat
    13381 tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt
    13441 tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat
    13501 atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc
    13561 gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
    13621 agtagtgctg gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca
    13681 gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca
    13741 cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca
    13801 cggcactcag tcacagcttg aatttctccc cgaacgccat actgacttta tcttcgcggt
    13861 actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct
    13921 gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc
    13981 tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
    14041 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat
    14101 tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc
    14161 gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag
    14221 gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc
    14281 ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac
    14341 tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg
    14401 atccgtctcg atttagccag gcgggactgg cagcaatcta tgatgccgaa ccaggcagta
    14461 acctgacggc ctctggcgaa gctttcgatc cgacaaagct gacggcggcc catccaacgc
    14521 ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc
    14581 gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag
    14641 ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc
    14701 aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttatg
    14761 ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc
    14821 agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc
    14881 cggtaaccag tagcggtttc ctcggcgctc aaacgacctt agcgcctggt gtactggaag
    14941 gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa
    15001 cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg
    15061 tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac
    15121 agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg
    15181 gcccattcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc
    15241 aattacagtc atttattact accgcgcagt agcgtaaaac aggcatctga tgtgtcaatt
    15301 tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg
    15361 ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15421 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15481 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15541 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15601 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15661 gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15721 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15781 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15841 gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15901 gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg
    15961 ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg
    16021 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    16081 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16141 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16201 tgatttctgc ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16261 tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16321 tcgcctcaga accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16381 acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16441 acagcagtga attgtatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16501 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16561 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16621 aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16681 ctcccacctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16741 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16801 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16861 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16921 cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt
    16981 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actctcctct
    17041 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    17101 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccggg
    17161 ctatgcatga attcaccgat acccgcgata atagtaccct tgatgaaatc tggctggtcg
    17221 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17281 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17341 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17401 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17461 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17521 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17581 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17641 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17701 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17761 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17821 attttcttta tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg
    17881 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17941 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    18001 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    18061 tatttacaac cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg
    18121 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc
    18181 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccagg
    18241 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18301 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18361 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18421 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18481 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18541 atgttctgtt caccgacaat atggtgttaa tttgccgcaa gggaaatcca ctactctctt
    18601 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagggc
    18661 aaaatttcag cggtctgcgg cagagagttc aagagatgtt tccggaccgg caaatcaatt
    18721 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18781 ttattcccag ccgtttttat aacctgttta gtcgttgctg gccgctggaa aaactacctt
    18841 ttccgtcctt aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18901 gtgatcccat cctgcacgga gtaatagatg ttattttgaa aaagtattgt tacagccaga
    18961 tcacagtgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
    19021 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
    19081 gataattaca acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac
    19141 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
    19201 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
    19261 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagctgc
    19321 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
    19381 atgcttcaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
    19441 tccgttctgt gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
    19501 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
    19561 tgatgacctg cgcgatggcg gtgcccaaca ctttgcggat tgtattactg ccattcggga
    19621 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gaatggatcg
    19681 tgctctggat attctgactg caacgccccc agatgtgttc aaccataacc tggaaaacgt
    19741 accgcgtatt taccggcagg tacggcctgg cgcagattac aactggtcgc tgaagctgct
    19801 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggtct
    19861 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
    19921 gatgttaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
    19981 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
    20041 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat
    20101 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
    20161 aagcgggttt tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct
    20221 ttatgagaga gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg
    20281 tcatcattca tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta
    20341 cgtaacttct tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta
    20401 atactaatct cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa
    20461 aaaccagaga atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt
    20521 gaatcaagcg ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt
    20581 ttaagatggg cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa
    20641 tgagtgcagg catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc
    20701 tttgcccgat atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca
    20761 gcgcacgagc ggcaagcaac gttgaccagt gatgctcttt gagcggccca cgaacccagg
    20821 cggcaggaag taccaggatt tcagctccct gtaaagcctg tgccagcgcc agctctggaa
    20881 agcgtaagtc ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag
    20941 ctatttcatt accagcatca acatggcgtg attcctgaat ggcaaatgca tcatagagat
    21001 gcagtttggc ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg
    21061 cgcgccccgg cgttgaagga agatgaatcg tcagaattgt cgtcatcatg ttacgtttac
    21121 tttctcgccg taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata
    21181 gatctgcatc atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt
    21241 cgttttccgc cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag
    21301 atgtaacagc aaactgtcct gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa
    21361 ttcgccaacg acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat
    21421 tttgcagtgt tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtagcg
    21481 agatggctgt taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg
    21541 gcagcaaacc tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg
    21601 acgaacattg atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca
    21661 accttctcaa cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg
    21721 gcattactga acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc
    21781 tggctgtttt cggcctcaac cgcacactaa agaccaaaaa aaacccgctg attaagcggg
    21841 ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg
    21901 gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta
    21961 gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa
    22021 ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt
    22081 acctttacat gaaaaataga cacaaatgct gtgtcggtta tcagtacacc aattgtggta
    22141 cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttgc
    22201 ttattttagt tattgatttt aaagaagtta ctaaaacttc atttgcctca aaactgaaag
    22261 cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga
    22321 aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa
    22381 ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca
    22441 tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc
    22501 cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc
    22561 gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg
    22621 taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt
    22681 tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc tctcatctgc gttagcaaaa
    22741 actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc
    22801 acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac
    22861 aattaacata accttaatag acccaacata aagaataatc tgaatagctg ctctatttat
    22921 ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg tcgttttctg
    22981 cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg
    23041 aaaaattatt ttattttttt aacgagatac aacaatcatc ttaacgaagt atataatata
    23101 tatcgtcaac ccatgctcat attagattat tttttgagcg cacagccgca gctaacacca
    23161 ttgcaattaa caaatttgca tcaatccacc atcaatttac acacattatt atgtgataat
    23221 tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagcaact tcaatgtctt
    23281 aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg catatttttt
    23341 ccttaagctt taggaagttt ttattattta ctttgggcct ggagacagga aaaattatgc
    23401 tgacattcat tgaactcctt attggggttg tggttattgt gggtgtagct cgctacatca
    23461 ttaaagggta ttccgccact ggtgtgttat ttgtcggtgg cctgttattg ctgattatca
    23521 gtgccattat ggggcacaaa gtgttaccgt ccagccaggc ttcaacaggc tacagcgcca
    23581 cggatatcgt tgaatacgtt aaaatattac taatgagccg cggcggcgac ctcggcatga
    23641 tgattatgat gctgtgtggc tttgccgctt acatgaccca tatcggcgcg aatgatatgg
    23701 tggtcaagct ggcgtcaaaa ccattgcagt atattaactc cccttacctg ctgatgattg
    23761 ccgcctattt tgttgcttgt ctgatgtcac tggccgtctc ttccgcaacc ggtctgggtg
    23821 ttttgctgat ggcaaccctg tttccggtga tggtaaacgt tggtatcagt cgtggcgcag
    23881 cagctgccat ttgtgcctcc ccggcggcga ttattctcgc accgacttca ggggatgtgg
    23941 tgctggcggc gcaggcttcc gaaatgtcgc tgattgactt cgccttcaaa acgacgctgc
    24001 ctatctcaat tgctgcaatt atcggcatgg cgatcgccca cttcttctgg caacgttatc
    24061 tggataaaaa agagcacatc tctcatgaaa tgttagatgt cagtgaaatc accaccactg
    24121 cccccgcgtt ttatgccatt ttgccgttca cgccgatcat cggtgtactg atttttgacg
    24181 gtaaatgggg tccgcaatta cacatcatca ctattctggt gatttgtatg ctgattgcct
    24241 ccattctgga gttcctccgc agctttaata cccagaaagt tttctctggt ctggaagtgg
    24301 cttatcgcgg gatggcagat gcgtttgcta acgtggtgat gctgctggtt gccgctgggg
    24361 tattcgctca ggggcttagc accatcggct ttattcaaag tctgatttct atcgctacct
    24421 cgtttggttc ggcgagtatc atcctgatgc tggtattggt gattctgacc atgctggcgg
    24481 cagtcacgac cggttcaggc aatgcgccgt tttatgcgtt tgttgagatg atcccgaaac
    24541 tggcgcactc ttccggcatt aacccggcgt atttgactat cccgatgctg caggcgtcaa
    24601 acctgggtcg taccctatca cccgtttctg gcgtagtcgt tgcggttgcc gggatggcga
    24661 aaatctcacc atttgaagtc gtaaaacgca cctcggtgcc ggtgcttgtt gggctagtga
    24721 ttgttatcgt tgctacagag ctgatggtgc caggaacggc agcagcggtc acaggcaagt
    24781 aaatagtaat accggaggca agtttcctcc ggctttatca tgtcacccac tgtggtatat
    24841 gcgttgtggt ctgccaactt tgccgtgaac aatttcagca ataatcagat ggcggctggc
    24901 gcaatattca agataacgcc tggcagtggt gcggctgatg gtcagtgcct gcgccaccgt
    24961 ttctgccgta tgttgcacac caggctcttt aaacagtttt cgcaccgcgt ttagcgtcaa
    25021 ggggtcaatg ccggtcggca gttcgtcttt gggttcaccg cgagcatagg cattaaacat
    25081 ctcatcaatt tgcttctggc tggcgctatc aatactttcc agcatatgtt tacgctggcg
    25141 gaaacgggtt agcgtttgcc ccagtgtatt gattcacttg aagtacgaaa aaaccgggag
    25201 aacattggat tattcgggat ctgatgggat tagatttggt ggggcttgca agcctgtagt
    25261 acaaatttta gccgttaacc aatgaaacgc gaaagatagt aaaaaattgc ttttgtttca
    25321 ttgaaaaaac gaaaaacaaa aacactataa atcatttcaa tcatagcttc aaaaaacgtt
    25381 caaaaccgat aacaaccagg ctgtcaccaa atgactcata tcacaaatca gctcat
//
LOCUS       DADMFL010000055        25068 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.055, whole genome shotgun sequence.
ACCESSION   DADMFL010000055 DADMFL010000000
VERSION     DADMFL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.055"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..315
                     /locus_tag="JNZ27_002235"
     CDS             <1..315
                     /locus_tag="JNZ27_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8664562.1"
                     /translation="ILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAK
                     ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQA
                     TA"
     gene            complement(359..4976)
                     /locus_tag="JNZ27_002236"
                     /pseudo
     CDS             complement(359..4976)
                     /locus_tag="JNZ27_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5069..5716)
                     /gene="rnt"
                     /locus_tag="JNZ27_002237"
     CDS             complement(5069..5716)
                     /gene="rnt"
                     /locus_tag="JNZ27_002237"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY8664563.1"
                     /translation="MSDNTQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5819..6226)
                     /gene="gloA"
                     /locus_tag="JNZ27_002238"
     CDS             complement(5819..6226)
                     /gene="gloA"
                     /locus_tag="JNZ27_002238"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY8664564.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6307..7404)
                     /gene="nemA"
                     /locus_tag="JNZ27_002239"
     CDS             complement(6307..7404)
                     /gene="nemA"
                     /locus_tag="JNZ27_002239"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY8664565.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDTGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7441..8040)
                     /locus_tag="JNZ27_002240"
     CDS             complement(7441..8040)
                     /locus_tag="JNZ27_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8664566.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8143..8382
                     /locus_tag="JNZ27_002241"
     CDS             8143..8382
                     /locus_tag="JNZ27_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY8664567.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8431..9327
                     /locus_tag="JNZ27_002242"
     CDS             8431..9327
                     /locus_tag="JNZ27_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY8664568.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9408..9929
                     /gene="sodC"
                     /locus_tag="JNZ27_002243"
     CDS             9408..9929
                     /gene="sodC"
                     /locus_tag="JNZ27_002243"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY8664569.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9930..11536)
                     /locus_tag="JNZ27_002244"
                     /pseudo
     CDS             complement(9930..11536)
                     /locus_tag="JNZ27_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11473..11911)
                     /locus_tag="JNZ27_002245"
                     /pseudo
     CDS             complement(11473..11911)
                     /locus_tag="JNZ27_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983454.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fusaric acid resistance protein"
     gene            complement(11808..12695)
                     /locus_tag="JNZ27_002246"
     CDS             complement(11808..12695)
                     /locus_tag="JNZ27_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8664570.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIHAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALVQGHAGAMALCV
                     TQYHCHVSGADGCLLFESG"
     gene            complement(12698..12934)
                     /locus_tag="JNZ27_002247"
     CDS             complement(12698..12934)
                     /locus_tag="JNZ27_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY8664571.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13135..13569
                     /gene="slyA"
                     /locus_tag="JNZ27_002248"
     CDS             13135..13569
                     /gene="slyA"
                     /locus_tag="JNZ27_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY8664572.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13616..14083)
                     /gene="slyB"
                     /locus_tag="JNZ27_002249"
     CDS             complement(13616..14083)
                     /gene="slyB"
                     /locus_tag="JNZ27_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY8664573.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14357..15466
                     /gene="anmK"
                     /locus_tag="JNZ27_002250"
     CDS             14357..15466
                     /gene="anmK"
                     /locus_tag="JNZ27_002250"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY8664574.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15570..15898
                     /gene="mliC"
                     /locus_tag="JNZ27_002251"
                     /pseudo
     CDS             15570..15898
                     /gene="mliC"
                     /locus_tag="JNZ27_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            15957..16613
                     /gene="pdxH"
                     /locus_tag="JNZ27_002252"
     CDS             15957..16613
                     /gene="pdxH"
                     /locus_tag="JNZ27_002252"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY8664575.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16742..18016
                     /gene="tyrS"
                     /locus_tag="JNZ27_002253"
     CDS             16742..18016
                     /gene="tyrS"
                     /locus_tag="JNZ27_002253"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY8664576.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18078..18944
                     /gene="pdxY"
                     /locus_tag="JNZ27_002254"
     CDS             18078..18944
                     /gene="pdxY"
                     /locus_tag="JNZ27_002254"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY8664577.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(18988..19593)
                     /gene="gstA"
                     /locus_tag="JNZ27_002255"
     CDS             complement(18988..19593)
                     /gene="gstA"
                     /locus_tag="JNZ27_002255"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY8664578.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19699..21201)
                     /gene="dtpA"
                     /locus_tag="JNZ27_002256"
     CDS             complement(19699..21201)
                     /gene="dtpA"
                     /locus_tag="JNZ27_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY8664579.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(21812..22447)
                     /gene="nth"
                     /locus_tag="JNZ27_002257"
     CDS             complement(21812..22447)
                     /gene="nth"
                     /locus_tag="JNZ27_002257"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY8664580.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22447..23142)
                     /locus_tag="JNZ27_002258"
     CDS             complement(22447..23142)
                     /locus_tag="JNZ27_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY8664581.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23146..23766)
                     /gene="rsxG"
                     /locus_tag="JNZ27_002259"
     CDS             complement(23146..23766)
                     /gene="rsxG"
                     /locus_tag="JNZ27_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY8664582.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23770..24828)
                     /gene="rsxD"
                     /locus_tag="JNZ27_002260"
     CDS             complement(23770..24828)
                     /gene="rsxD"
                     /locus_tag="JNZ27_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY8664583.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24829..>25068)
                     /locus_tag="JNZ27_002261"
     CDS             complement(24829..>25068)
                     /locus_tag="JNZ27_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8664584.1"
                     /translation="AKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQAN
                     AEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         6189 a         6482 c         6551 g         5846 t
ORIGIN      
        1 atactggatc agggctggta tgaaatgcgc cgccagcttg agtacaagca gctctggagt
       61 ggcggtcagg tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt
      121 catacagcga aagaaaaccg cctgtcacaa agtaaattca gatgccaggt atgtggatat
      181 acagcgaacg ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt
      241 gcctgtggag agatggtgca gtcaggccgc tcgttgaagc aggaacccac cgaaatgatt
      301 caggcgacag cttgaacgta gcagggaacc acgtctttca gggcgtggag gatgtcaact
      361 atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca
      421 tcggcgtttg ccggaccgga agatcattca cttctgttag cgtaaagcgc agccttggtt
      481 cgcgacgcag tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat tcctcctttt
      541 cctgccagac cagcattttt ttgccgcctt gcgccagata gagtaacaat ttgccgccag
      601 aaacgaccac cagcgcaccc gcccgacgcg ttggtaccag cgctgccgga tgtgcaggcc
      661 agggcagaag atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat
      721 agtggcgtgt ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt
      781 cagcgaattg cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt
      841 cttccatact tcgacaaagt gtttgcatcg atggaaaccc gccagggata ttttcggcta
      901 tcaccgcctg acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca
      961 tcctttcggt atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg
     1021 gtgtgttgta agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag
     1081 cccggtgact gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta
     1141 acggtgccca aatatcgctg gtaatgacgc cttgccagac cagcgcccat aacgcctctt
     1201 gcagtgccga ggaatcaacc gattcgccga ttttatcgcg tatccgttgg ctgatttgtt
     1261 gtgcaaacca ggctcctccg tcagccagaa cagcgattat cgcttgctgc agcgccgaac
     1321 gattcgcctg atccgcttcg gcgggagtga acgattctgc ggcatatttc tgtagatgca
     1381 gtgccaccag gccgtcatct tcacccagct tttttttgtc ccgaccagat aaccgctccg
     1441 gttgccagta attcatcgag catttctggc gaatagtcgc gtacgcgggc aggcaggatc
     1501 tggctttccc agagtgaggc gggtaaaccg actccggcaa gctgttcgat cacccgcatc
     1561 acgccatcta cgccctcata aacgcctggc gatgttgagg caaagagcgc cgggctacca
     1621 tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt cgtggctgca
     1681 acgggacgcg tcgcttctct ggcggcttgc agcgagcgca aacgcagacg acgaaatact
     1741 tcatcgctca cccagatgtc ttgttgcaga ttcatcacca gaccctgttc acgcagttgc
     1801 tgaagctgct cttcgacaat ggcaataccg agactaaact catgagccag ttgttcagcc
     1861 gtgaccagag catgagcccg gagatagcgc agaaagaggt cgcgaagcgg gtaactgact
     1921 ctatgtaaat aaatctctgg caatgactct ggtagtcgta cgccgagggc atcacgcagc
     1981 ctggcggcat catccataca ggcaagacgc tcctgtccgc taatcatcgc agggaagatt
     2041 cgttttactg caagaagatt ttccagatac gacgcaacct cttcactgct gcctgtatgc
     2101 cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc gaacaatcct
     2161 tcttcacctt tcgctcttct gccaggagcc agtcgttgca actcttcttc cacctggcga
     2221 atgacctgcg ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt gcgcagtaac
     2281 tcgctgtcca gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc gctctggtac
     2341 ataaattccg cgacatagcc gaacaataaa cttgtggcga aaggcgaggg cgtagtggtc
     2401 gttacatcgg atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg ttccagtgcg
     2461 ggaagatcat aaacatcttg cagacattcg cgtagggttt cgagaatgac cggaaaatcc
     2521 ggatatccct gagcgatttc cagcaactga ctggcgcgca gccgttgttg ccaaagcggg
     2581 gtgcgatggc ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca ttcgcgaaaa
     2641 cgggcggcga aaagtgccga gctgcctacc gcctcgcgga caatttgcag caacttttct
     2701 ggttcaaaca aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg aatgcgcgca
     2761 acaatgccgt catcactggc gaccacagac gcgtcagcgc cccatagggc atgtattcgc
     2821 ccggcaatcg ccagcgccca gggttcatgc acccgtcttc catagggaga gtgcaaaata
     2881 atacgccagt cgccaatttc atcacggcac cgttcgagga ccagatggcg actccccgga
     2941 acgatgcccg tcgcgttgcg ttgctcgtca atcaacccct gaatattggc gttcgtattt
     3001 tcttctgcca gccacggggg aatagtcccg gaaaagaacg cgccatccgc cagtaaatga
     3061 agaaaatcgc cgatcatctc gcctaattca gccggacgtc cgttaccttc accacgccag
     3121 aaggggagcc gggcagaacg acccggagca ggagtcacaa tcacctgatc gcgggtgatt
     3181 tgctggatcc gccatgaggt agcgccgaga gtgataatgt cgttcacccg cgactcatat
     3241 accatctcct catccagttc acccacccgc cgcgaaccgg ccttttcttc accttcgggt
     3301 aataacacgc tatacatgcc acgatccgga atggtgccgc cgctggtaac cgccaacaat
     3361 tgagcgccag gtcgggcggt caatatcccg gtctccctgt tccagaccag tttggggcga
     3421 aaagcagaaa aatcgccaga gggatagcgc ccggaaagca tatccagcgt ggcgtcaaaa
     3481 acacgtcttg gcagatcttt ccacggtgcg gcacggcgta cgcgggagta ccattcgtct
     3541 acctgtaatg catccatcgc cgcggcggca acggtttgct gcgcaaggac gtcgagagga
     3601 ttatgcggtg gtgtcaggtt ttccagcctg cctgcgaaca tacactctac aatgactgcg
     3661 gaatcgacta aatcacgccg ggtacggggg aaaaacagcc ctttagatac accgccaacc
     3721 tgatgtccgg cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg cgttgcctcc
     3781 tgaatcacca gatccaccgc gcccatatca atccccagtt caagactgga ggttgcgacc
     3841 acgcagcgta attcacccga tttcagcgcc tgttcggtga ttgctcgttg ttctttagag
     3901 acggagccgt ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg gttagaggtt
     3961 gcgccggagg tcgactcgaa atgggccgca tcaacggcga tagacgggga acgctgtaag
     4021 cgtgcggcgt aaagctcatt taatcgtgcc gtcagttttt ccgccagccc gcgtgaatta
     4081 gtaaagacaa tggtcgagcg atggcgcaac acttcatcaa ggatacccgt ttcaatatat
     4141 ggccagatgg agccttcccg gccggcatgg ctgtcttcgc cggtgccgct ggcgaccgat
     4201 gagacatcat ccatattggc gaccggtacg acaattcgta tctgcggatg gcgcattgcg
     4261 ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac atcgctggct
     4321 gagcgcacag tggcagaaag gccaattcgc tgtgctgagg tgtggagcag cgcatcgagc
     4381 cgctccagac ttaacgccag atgcgcacca cgtttactgc ccgctaccgc gtggacttca
     4441 tcaataatta ccgtttcgac gccgtgtagc gtttcgcgcg cgcgggaggt cagcatcaga
     4501 tagagtgatt cgggtgtggt gatcagaata tccggcggat tacgggtgag tttgctgcgt
     4561 tcctgtgcag gcgtatcgcc agtacggatc cctacgcgaa gattgacttc cgtttcgccg
     4621 cgccgccgcc gttcatcggc aatacccttc aacgggatct gcaagttgcg ctgaacgtcg
     4681 gtgcccaggg cttttatcgg tgaaatatag aggatgcgtg aggttttacg cttatgcgcc
     4741 tcgcgggtat cttcgccgcc ttcgcggaag agccgatcga gggcgtagag aaatgctgcc
     4801 agcgttttcc cggagccagt cggtgcaatc accagcgcat gttcgcctcg cgccgccaca
     4861 tgccaggttt gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa ccagtcgcgg
     4921 gtcgccggtg aaaacacgtc cggcaggagc gatgaagggt ctggattatc tgccattaac
     4981 gctattcccc gtgaatatca tgcttgatgc gtggaatagc ctcactatag atgcctgaat
     5041 cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc
     5101 ctcccagacg tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt
     5161 cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct
     5221 ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga
     5281 aagggtggaa cgggttacgt ttcagcgagg cgcgttctgc ggcggccatc ataaagctgt
     5341 gatcaaaatt ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac
     5401 ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc gcgccgcgat
     5461 cgggatcgtt cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa tttgcgccga
     5521 caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca
     5581 ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg
     5641 tttcaacatc gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt
     5701 gagtgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa
     5761 atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt
     5821 agttgcccag accgcgaccg gcgtctttct cttcgattaa ctcaattttg taaccgtccg
     5881 gatcttccac aaacgcgata accgtagtac cgccttttac cggacccgcc tcacgggtca
     5941 cgttaccccc gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg
     6001 cgatgtgacc ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt
     6061 caatcaccgc ttcttcggtt tccgggccgt agccaacaaa cgccagtgag tatttgtatt
     6121 ccgggttttc gctggtacgc agcagtttca tgcccagcac tttggtataa aaatcgatgg
     6181 agcgttgcaa atcgccaacg cgcagcatgg tatgaagaag acgcatcttt ttatcctcaa
     6241 aatggctaac agattcaaaa tgttgtttta gtatagcggc ggctttacgc cgctcgcaat
     6301 gttggattac aacgtcgggt aatcggtata gccttccgcg ccgccaccgt agaaactttc
     6361 ggcacgctgt gggttaagct cagctttgag ctgcaagcgg gcgaccagat ccgggttcgc
     6421 aatccagtca cgaccaaatg ccaccgcatc aattaaccct ttgccgatca gcgtttcagc
     6481 tttttctact gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt
     6541 ttcgcggaac gcatcagtat acggttcacc ccccgcccaa tctggttctg acatatgcag
     6601 ataagcaatg ccgcgtttac ccagttgttc aatcagatac agtgcatcgg cttcttcatt
     6661 cgggccgtta tctgtgttct ggaaagtacc gattggcgaa acgcgaatgc caatgcgatc
     6721 ggcaccccat tcttcaatcc cggtatcgac cacttccagt accaaacgtg cgcgattttc
     6781 cacgctgccg ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag
     6841 caaataaccg tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc
     6901 aatggcctga cggaaatcat tgacgatacc cggaatctct tccagttcaa gcgcacgcgg
     6961 catggatgtt tcaacgcgga tcgcccgacc attttcatcg cgcagagaag tacgtgttcc
     7021 cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga
     7081 aatgcgtccg gtgtgccaca gctgcaaggc catatgacca ttttcagcat gaacgccagc
     7141 ggtgattttt ttccatgcgg caatttgctc cggactatgg atgccaggcg cacctgcata
     7201 tccttttgcc tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg cactggcacg
     7261 ttggcgatag tattccgcca tcaacggggt aggaatgtca cccggttcaa tactgcgcag
     7321 acgcgtcagc ggtgccataa aaatacggtt tgccgccgtg atcgcgccca ctttcagtgg
     7381 ggaatacagt ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg
     7441 ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc
     7501 gaaactgcgc gaaattttgg cctgcagatt cgcgccaagc catagtgcgt aaagcacttg
     7561 tgcctgttgt agcggttcgc cacaaaaggt taaacaatgg ttctcacggc cattttccag
     7621 agcctgcgag agcagggcga tcacgccgcg agcaccttta tccatcgcgc tgcgcatatc
     7681 ttctgacaga tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc
     7741 atgttggcaa aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt
     7801 accttcgccg gattgcagca actcagccag tcgctggtga tatgcggcgt aatgacgctc
     7861 aagcatggca acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg
     7921 cacttcagcg gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca
     7981 aagttgctcg cccgtcgcca agagatgttc gcgagtatca tgttcggtgt gtttgttcat
     8041 gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta
     8101 acagcttcaa taatggtaat aatcttatct gaggagggcg aggtggcgga gcaattagag
     8161 ttctttcccg tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt
     8221 cgcggctgtt ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa
     8281 aaacaagagg tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg cgcaaataag
     8341 ccagcgtcat cagacgaacc ggagcaacca tcactctttt gaacctaatc ttgcgtatac
     8401 tcctctcata tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccggaa
     8461 ggcccggagt tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc
     8521 gcccgccagc tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac
     8581 catgctgata tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc actgaaactg
     8641 gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg
     8701 cgtgaagaaa acgtcattgg tcattacatc actgaccgca atcacatcat taagagcgcc
     8761 gaacagtcgc taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca
     8821 gacccgttaa tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc
     8881 aaagtgcgtc attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca
     8941 cgtctgccgt ttacccttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta
     9001 ctgctggatg gcacgctcga ccaactacaa caactgcgtg ttcgtccgat ggcgtggtcc
     9061 tgccttggtg gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg
     9121 gctgtggtgg cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcttgggta
     9181 ttacgtttac cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg
     9241 gcagctgtcg aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa
     9301 gctgcactgg ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt
     9361 gtcactggtt tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta
     9421 gtctggccat tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg
     9481 agatgaacct cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg
     9541 aaaccgataa aggtctggag ttttcacccg atctgaaagc attacccccc ggtgaacatg
     9601 gcttccatat tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg
     9661 ccgcggaatc cgcaggcggg catcttgatc cacaaaatac cggtaaacat gaagggccag
     9721 aaggtaccgg gcatttaggc gatctgcctg cactggtcgt caataatgac ggcaaagcta
     9781 ccgatgccgt catcgcgcct cgtctgaaat cactggatga aatcaaagac aaggcgctga
     9841 tggtccacgt tggcggcgat aatatgtccg atcaacctaa accgctgggc ggtggcggtg
     9901 aacgctatgc ctgtggtgta attaagtaat tacgaggcca gcgtaccttg cggtggtgct
     9961 tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa ttgccgccag ctcacgggcg
    10021 gcaggttgat gatgacgggc aaggctgtcg caaatccgct gtaattcttc aagtgtggcc
    10081 gccagtgatt tttgctgaac gccacgctca ctcatcactc cccgcaacag tgaaatacag
    10141 ttatcccgta ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa ttgccagaca
    10201 acatgagaac agttcagcag cactacaccc cagcgtaata accaacggcg agccagcgca
    10261 tcctggctgt tactcaactg actgacgtga tgataagtga gcgattcaaa ttcgctttca
    10321 ctcagtgttg gatggcggct tagctgatcg acaaaatccc ggcgcagcgc gcgaatatgg
    10381 cggcggcttt tacgagcatc cgatcctgga cgcagaatgg cgaacgctag ccaagccagc
    10441 gcaacgccaa cgatttttgc cagattatcg ttaagaaaat cagcaaaatc atacaccggg
    10501 ggattagtga cagcgataaa cgaacccata aaacaatcag ttgcccccac agtgcggcaa
    10561 atttgggcat ctgcaattta agaagctgca ttgtcgccag cagtggaaag agaaacagta
    10621 aaaattgcca cagatcgcta atctggacca tcaggccaaa tttgaccaca aagctgaata
    10681 gcgaaagtaa caccagcgtg cgcatcagca gtgacaacga cttaaacggt gctgcgacgg
    10741 cggagtagag tacgcagcta attgctgcca gcgttaatgc attggcaccg gcatcccatt
    10801 gcgatgcaat actccatgcg ccaatcatca tcaacgtaca aaatgtacgc agcccgctcc
    10861 acatagcttc ggcgttgtcg gtatgacgag ccagagcgga tgtccgtgcg agtctggcat
    10921 gatcatctac accgctttgc agacgatgta attcctgact actttgcaga taaaggcggc
    10981 aaaaataacg tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg ttggtcgggc
    11041 gtagcggggc gataatacgt gcgacggtgt aagcatctgt ttgcgaactg gcgagcgccg
    11101 tcagtaattg ttcgagaatt tctcgcgtgg cacctggcgg tgagggccag ttgagcaaca
    11161 tacgtcgcag gctggagatg acactggtca tacgtaattg ctggtggagt agcgcattaa
    11221 ggcgcgcgtt ttgctggcga aaacgatagt ggctccagaa agcctggata cgcagcaaat
    11281 tcatggtcag tatctgccca atcaccccct catgtgctgc acgaatggca tcgtttgttt
    11341 caggctgcca gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt ttcaatgcag
    11401 ttaaaagggc agtagcatcg gaactgctag gcaggatcat catcatcatg ccgccgcaca
    11461 aaataccgac tatcacctcg caaacgcgcg cctgagcgat atcccacagc tggctggcct
    11521 cagtaatatt aaccatcgga aaggcgatga tggcagccgt gtagcccgcc agttgaaatg
    11581 catacgcgac gttattcgtg aagtgcgcac aggcctaggt acaaaagcca agccacgccg
    11641 acatgctcaa tagaaaaaac cacggctcat tgagcgtatg tccggcaaga agcagtgccg
    11701 caatggctcc cagtaaactg ccagcgatgc gtccgaggct tttgctgatg acaccgccaa
    11761 cggtgggaaa gctaaccact gcagccgagg tcatcgccca atagggttca tccagattca
    11821 aataataggc aaccgtcagc gccagacaca tggcaatggt attgcgtaac gcataacgcc
    11881 attgcgccag cgtggccctg aaccagcgca aagcggacgg gaacgcgttg ggcgagacga
    11941 acccagggga cgttaggttt cacatccgga ataaggcttg agtcactttc aacgctctga
    12001 tcataaatcg cccgaccgat actggaaacg tgaccctgta acgttttgtt atcactatag
    12061 agggtaattt gtgctggtgc accttcgcgg atatggcgca acttggtttc ttcaaaataa
    12121 ccaatgacgt aaaacgagtg gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg
    12181 taatcgccga tgcgggtagt gaggttagtc acccatccac tcaccggggc gcgaatttcc
    12241 gtttgcgcca gttgccattg tgtctgtttc agcgtcgcct gtgcggcatt aacactggcc
    12301 tgcatcgctt taacattgag gttagcggta tcgagttctt cggcaaaaat aaaattttgt
    12361 gagagatgac gacgacgatt cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc
    12421 gccagctgag cctgtgcgtt cagctcggcg atctgaaagg gcgttttatc gatcgtgagc
    12481 agaagttccc ccgcgttaac cagttggttg tcttttatat tcagctcaac aatacgtccc
    12541 gacacctgtg gggtgataga aacctgttct gcgtgtattt tcccgtcgcg tgtccacgga
    12601 gattgcatgt aataattcca cagccaccat ccggcaagaa ccgcaactat cgctacaatg
    12661 attgttgaga aatacttaat tgttttaatt gacatagtta ccacgcaatc agtattgcca
    12721 gagcaaggca aacgcaaatc gcaaacagcg ataaatccat taacaaggga tgccagatgt
    12781 caccggcgta gatccagcca cgaagcagac ggtgtacgac aagccagatg acgaatccaa
    12841 acgcgaatgc tttgaaaaac ggaggaaaat agacggacgc accgaacacc agatcttgta
    12901 agggcaatcc tgttgcattg agcataaact tcacaaagta gattccttta cgaccgaata
    12961 tattgcgtta gattaataaa tattctttaa gtgcgaaaaa tttacgcgca atttctgaaa
    13021 aatatgtctg acggtaacca aatgcagcaa tacatttgtt ttagcaatac aattgctgca
    13081 cactattcta aaagccgcat aatatcttag caagctaatt ataaggagat gaaattggaa
    13141 tcgccactag gttctgatct ggcacggttg gtgcgcatat ggcgtgctct gatagaccat
    13201 cgcctgaaac cgctggagtt aacacaaacc cattgggtta cgttacacaa tatccatcag
    13261 ttacctccag accagtcgca aattcaactg gcaaaagcga ttggcatcga gcagccatca
    13321 ctggtccgta ctctggacca actggaagaa aaagggttaa tttcgcgtca aacttgtgcc
    13381 agcgatcgtc gggctaaacg tattaaactg acggaaaagg cagagccact gatcagcgaa
    13441 atggaagctg ttattaacaa aacccgcgcg gaaatattac atggcatctc cgcagaggaa
    13501 ctggagcaac tgattacgct catcgcaaaa cttgagcata atatcattga gttacaggcc
    13561 aaagggtgaa atgaaggggg cgtgtggtca gtaacctgac cacacgcaaa cttatttagc
    13621 gcggagaaac ggtcacctga ctgccattgc tggccagtac gacacgttgg cccggagaga
    13681 aacgagtgtt gccttgtttc tgtaccacca tgatggtatt accatcgtct ttacgaattt
    13741 ccagctcgac accctgcgtt ttgttcattg cactctgtac gccctgacca gctacgccac
    13801 ctgcaacagc gcctgctgca gtagccagag aacgcccggt tccgccacca acagtattcc
    13861 ccaggaaacc accaagaaca gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc
    13921 cctgaatctg taccggacgt acgttaacga tggtgccata gctgacattc tgtacttgtt
    13981 tcgcttcaga agcggtataa acatcccctg acagggtgtc gttattaaca caaccgacaa
    14041 gagacagacc taccattgaa acaaccaata cgcgtttaat cactgaaaaa tctcctgttc
    14101 accatgaaac gccacgcaag catccctcat ggtcaaagta tatggcatat gtgaatccat
    14161 ggttacatgt tctaccaaat atgagcaaat cataatgaaa gtaggatcaa ccaattataa
    14221 acaaaacatt catgatgatt attgatttga agataatgtt ttactaccaa cggtaaaaat
    14281 cggtcattcc gcatggtatt cacgaaaagt ttatgtttga gtggtgcgca aacgtcagat
    14341 tgaaaaagga tgaactatga aatcgggccg ctttattggc gttatgtcag gcaccagcct
    14401 tgatggtgtt gatgttgtgt tggcgacaat tgatgaacac cgggtcgcac agctggcaag
    14461 cttgagctgg ccgatcccgg tgtctctgaa acaggctgta ctggatattt gccagggcca
    14521 gcagcttaca ctttcgcagt ttggacagct tgatactcaa ctcgggcgac tttttgctga
    14581 tgcggtcaat gccttgctta aggaacaaaa cctgcaggcg agagatatag ttgcgatcgg
    14641 ctgtcacggt caaaccgtct ggcatgaacc gacgggcgtg gcaccacaca ctttacagat
    14701 tggcgataac aatcaaattg tggcacgcac cggaattacg gttgtcggtg attttcgccg
    14761 tcgcgatatt gccttgggag gacaaggcgc accgctggta cctgcgttcc atcatgcctt
    14821 gctggctcac ccaaccgagc gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc
    14881 actgctcatt cctgggcagc cggttggcgg ctacgatacc ggtcctggta acatgctgat
    14941 ggatgcctgg atctggcgtc aggcaggtaa accttacgat aaagatgccg agtgggcacg
    15001 ggcgggtaaa gttattctcc cactgctgca aaatatgctc agcgacccgt atttctcgca
    15061 acctgcaccg aaaagcaccg gacgcgaata ctttaactac ggctggctgg agcgccattt
    15121 acgccatttt ccgggtgttg atccccgtga tgttcaggcc acactggcag aactcaccgc
    15181 cgtgaccatt tctgaacaag ttttgttgag cggtggctgc gaacgattga tggtatgtgg
    15241 tggaggtagt cgtaatccgc tactcatggc gcgtctggcg gtattactgc taggcacaga
    15301 agtcaccacc accgatgccg ttggcattag tggcgatgac atggaagcat tggctttcgc
    15361 ctggcttgcc tggcggacgc tggcgggatt accaggaaat ctgccttccg tcactggcgc
    15421 aagccaggag acggtactgg gggctatttt ccccgccaac tcgtgatatc agaattaact
    15481 gaattttttc ttatcgtgtc tgccgttaca cttggaagat taaggagggt atttgccctc
    15541 cggaccagga aagtcttcgg gatatgacta tgaaaaaact gttaattatc attttgccgg
    15601 tccttctctc cggttgtagc gcctttaatc aactggttga gcgaatgcaa accgatacgc
    15661 tggaatacca gtgtgatgaa aaaccgttga cggtcaaact gaataatccg cgccaggaag
    15721 tcagttttgt ttacgataat caactactgc atctcaaaca gggcctttca gcctagcctc
    15781 tggcgcgcgt tactccgacg ggatctatgt tttctggtcg aaaggtgagg aagcgactgt
    15841 ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta caaaatccac agcgttgaga
    15901 tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt
    15961 ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt
    16021 tacgccgccg cgatcttccc gccgatccat taaccctttt tgaacgttgg ctctctcagg
    16081 cttgtgaagc caaactggcg gaccctaccg cgatggtggt cgctaccgtg gatgaacatg
    16141 gtcagcctta tcagcgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt
    16201 acaccaacct cggtagccgt aaagcacatc aaattgaaaa taatccgcgc gttagcctgc
    16261 tgttcccgtg gcataccctt gaacgccagg ttatggtgat cggtaaagca gaacgacttt
    16321 cgactctcga agtgatgaaa tattttcata gccgcccgcg tgatagccag attggtgcat
    16381 gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg
    16441 agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagcttttgg ggcggttttc
    16501 gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg catgaccgct
    16561 ttttgtacca gcgtgaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg
    16621 caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct atgtctcttt
    16681 cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt
    16741 gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac
    16801 ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt
    16861 cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg
    16921 cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg
    16981 cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg
    17041 ggtggacaaa atccgtaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc
    17101 tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg
    17161 cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg
    17221 tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg
    17281 ttatgacttc gcctgtctga acaaacagta cgatgtggtg ctgcaaattg gtggttctga
    17341 ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt
    17401 gtttggcctg accgttccgc tgatcaccaa agcagacggc accaaatttg gtaaaactga
    17461 aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg
    17521 gatcaacact gcggatgccg acgtttaccg cttcctgaag ttcttcacct ttatgagcat
    17581 tgaagagatc aacgccctag aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca
    17641 gtatgtactg gcggagcagg tgactcgtct ggttcacggt gaagaaggtt tacaggcggc
    17701 aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg aagcggactt
    17761 cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg cagacctgat
    17821 gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta aaactatcgc
    17881 ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga
    17941 agaagatcgt ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga attactgtct
    18001 gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt
    18061 tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc
    18121 atgcgggtaa cagtgcggca gagtttccga tgcgccgcct gggcgcgaac gtctggccgc
    18181 tgaacaccgt tcaattttct aatcacaccc aatacggcaa atggactggc tgcgtgatgc
    18241 cgcccagcca tttaaccgaa attgtgcaag gcattgccat tgccgccatt gataaattac
    18301 acacctgtga tgccgtatta agtggctatc tgggatcggc ggagcagggt gaacatatcc
    18361 tcggtatcgt ccgtcaggtg aaagcagcga atccgcaggc gaaatatttt tgcgatccgg
    18421 taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtac
    18481 ggcacggttt gcctgccagc gatatcattg cgccgaatct ggttgagctg gaaatactct
    18541 gtgagcatgc ggtaaataac gtcgaagaag cggttctggc agcgcgcgaa ctcattgagc
    18601 aagggccaca aattgtgttg gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt
    18661 ttgaaatgct gctggtcacc gccgatgaag cctggcatat cagccgtcca ctggtggatt
    18721 ttggtatgcg ccagccggta ggtgttggtg atgtgacgag cggtttactg ctggtgaaac
    18781 tgcttcaggg ggcaacgctg caggaggccc tggaacatgt gaccgctgca gtctacgaaa
    18841 tcatggtgac caccaaagca atgcaggaat atgagctgca agtggtggct gctcaggatc
    18901 gtattgccaa accagaacat tacttcagcg caacaaagct ctgaaatatc tcatttcggc
    18961 ccacaacgac ctgtgggccg atggctatta ctttaagcct tccgctgaca gcgcgtcttg
    19021 tacttccgga cgttcagcca tacgttgcat aaatgccgca atgtgctcta acccttccag
    19081 attcagtttc accgcgtatg cccagcgcag aaccgtaaac agataggcat cagcaattgt
    19141 aaatctttgc ccgcagatcc agtgctcatc cttcagtgcc tcgttcacat attgcagctt
    19201 cttatccagc tgcgcgcgaa ctgtcggttt gtactcttcc ggtgtatctg ggcgaaacag
    19261 aggtgtgaaa cctttatgca gctcggtggc gatgtaattc agccattcga tggttttata
    19321 gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg gggacgctgt cggcaagata
    19381 ctgcataatc gcaacgcctt ccgtcagcaa agtaccatcg tccagcagca atgcaggcac
    19441 ctgcccctta gggttaacgg aaaagtaatc gtcaccgttt tcgagacgtt ttttcattaa
    19501 atccacactg acgagcgtaa aatcctttcc gctctcacgc agggtgatat gggaagcgag
    19561 agagcaggcg cccggtttgt agaacaattt catcggtaac tccttttgtc tgtggtttaa
    19621 agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta
    19681 aaagagtttc cctgaatttt acgctacagt agctttcgcc gctttgtctg cagcgtcatc
    19741 ctgcgtcatg cggtgcagtt taggcgcggt cagcagcatc agtactgcaa taacggcagt
    19801 agcgacacca atctgcaaga atacgcgacc atagacttcc agtgacatca gcgggtcggt
    19861 aacgttatcc ggcacagcca tcatacccgc aacataacca ccaatcaggt ttgcaccggc
    19921 agtggtcagg aaccagctac ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc
    19981 aaccattgcc agacccagac cagagatcat cagttccccg atgctctgca ggccatagct
    20041 tgcgaccagc cagctgacag acacgatacc tgcgtcagac gcgaatttcg cacccagcgg
    20101 cagaatcagg aacgcaccag aacacatcac catgccgatt gcaaacttgg ttggcatcgg
    20161 cagggtatca cccatcttgt tatagatagc ggccagaatc ggactaccga tgatgatcca
    20221 gaacgggttc agtgcctgat actgctcagg ttctacggcc agacccagaa tggagtgctc
    20281 aacgttacga atcgcaaaga agttcagtga cgttggcatc tggctgtaca gcacgaagaa
    20341 gatgattgct tcgagcatca ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt
    20401 catcgcgaaa gcttctttac cgaagataac cacaataccg aaggcaacaa cgcccagcgc
    20461 catacgcgca acttcctgat tgtgcagtag ccaggtggca acagcgatca gcgccacaac
    20521 accaataatg gtcagcaaca ggttacggta gttgactggt tcaaagtctg gttttgaacc
    20581 gtactgttta acccagcgtt ggcagaaggc gaagttaacg atagtgatca gcaggcctac
    20641 aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg gccagccacg gggtggctat
    20701 catagagaag aaagagccga tgttgacgga catgtagtac atggtgaatg caccgtccag
    20761 acgtgggtcg tttttctcat agcatgtaga aagcagagaa gacgggttag ctttaaacag
    20821 gccgttaccg accgcaatag ccgccatacc catataaaca ataccggcgt cgtgaccaga
    20881 ccaggcaacc agagcataac caatcgccag cacaatagcg ccgagcataa ttacgcgttt
    20941 agtacccagt accttgtcac ctaaccagcc gccgatagcg accagaccat aaaccagggc
    21001 actaaaggaa gagaaaaggg tgattgaatc cgcttcagac atacccagtt gtttaaccag
    21061 gtaaacagcc ataattcctt gtaggccgta ataaccaaaa cgttcccata actcaatcga
    21121 gaagatgaga tagaacgcct tcggttgttt gaaagcgttc aaactgacgc tttcagttgg
    21181 tttttggttt gcagtggaca cttttacctc tttttttaca tcccatatta acgggggtgc
    21241 tttctcgcgt tatgtctaaa aaacatacgc gcaattgtta tagtgggagg ggaaacggca
    21301 ggtaatgttc actatcttgc actttcagac aagtcctttg taataatctg ttacatataa
    21361 ctgaggcggg gaattcaacc attccgggaa atgttatcca gcgttaaatt tttcaaaaaa
    21421 cgtaacggca gattttttca ctgcattgag agggggatga tggcttgacg gagatatgtt
    21481 ctgctattta gcgtttttcc tagtgagata gtccagtttc tgaaaaatag ccagtgtaat
    21541 gttttgcagg tcaatatttg ttgcgtgtgt aaatttaaat cattttaacc tgattcttgc
    21601 aatgttttta cattgccaca gcatcgtgat cttgatcacg aatgaataga aaatttcgct
    21661 attaaaaaaa caattaacct gcatttttgg ttatccgcaa acggattacc tgatgataac
    21721 gctaagcaat ggcatcattg acgttaaaaa gatgttttaa aagaggataa agaaaggtta
    21781 tcaatggggt aatcggtgtt accccttttc ttcagatgtc aactttctct ttgtattcac
    21841 aaagatcttc aataatacaa gagccacagc ggggcttgcg ggcaatgcag gtataacgcc
    21901 cgtgcaggat caaccaatgg tggcagtcga ctttaaactc tgccggaacc actttcagta
    21961 gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa ttgagtacga ttacaaacgc
    22021 ggaaaatgtg cgtgtcgaca gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt
    22081 tggctgtttt acgacctacg ccgggcaggg cttcaagcgc agcacgatct tccggaacct
    22141 cgccattatg ttgttccagt aatatacggc aggttttgat gatattttct gctttgctgt
    22201 tataaaggcc aatcgttttg atataggttt tcaccccttc aacgcccagt tcaagcatcg
    22261 ctgcaggcgt attcgccacc gggtagagtt tcgccgtcgc cttattaaca ctgatatcgg
    22321 tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa aggcgaactg aaattaagct
    22381 cggtggtggg atgaggattg ttctcacgca ggcgagtgag gatctccaga cgttttgctt
    22441 tgttcatcag acattccctg tttcaccgtt tggcaatgca cgttcggcag ctgcttcagc
    22501 acggcgcttt ttcattcttt catcaatcag gtattttcct gccagcatca gtcccaagcc
    22561 aataaatgca cctggtggca gcatcgccag caggaaaggg gagtcggtgt ggaaaatctc
    22621 cacgcgtaat acttttgccc agctacctaa cagcgcatct gcaccgtcaa acaacgtgcc
    22681 attgccgata atttcgcgta gtgaacccag cacgaacatg gcgcaggttg cgcccgtacc
    22741 aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt ttggcggcga aggcttcagc
    22801 gcggcccaca acgatacagt tagtgacaat cagcggaata aaaatcccta atgattgata
    22861 caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat
    22921 catcacgtaa atgggaatgc ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt
    22981 caggttggtc agcgtcagta ccagcgtagt cgcaagtcct aaacccagag cgttagtggc
    23041 agtggacgtg accgccaata gcggacaaag gccgagcagt tggaccagtg cagagttgtt
    23101 tttccacaac ccctgaacaa taacgtcttt aatttcgctc acgctttatt ctccacaggc
    23161 aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc
    23221 attaaccacc gcgcggggag taatcgtcgc gccggtgaac tggtcgaaat caccaccatc
    23281 tttcttcacc gcccagtgcg catcatccgc accactgatt ttttttcccg caaaatgggt
    23341 gagccagtca gaaaggcgca attcgatttt atcgccaagc cctggcgttt cgtggtgctc
    23401 tgtcacgcgc gtgccaagta ccgtgccatt aaaatcggct cccaccagca gctgaatcgc
    23461 accggaatag ccatctggcg cggttgcttc cagaacggcg gctaccggtt tgtcatcctg
    23521 tttggcgatg taaacccgat gctcaccttt acctaactct ggcgcagtta ccagatagca
    23581 actctgtgcg agcgcattgt tatagcgttc ggctggcagc acctgaccaa ataacgcctt
    23641 ttgttgcaga ctggcctgtt cagcaattgt cgttttggtc atctggttga tggccgcagt
    23701 taaccctgta gaacccgctg caaatagtgc cagtgtaatg ccgtgttttc ggatagtttt
    23761 cagcatggtt taccctttgc gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag
    23821 aggaaccgtg atgttcgcca gcaggacggc aaaagccacg ccgtcaggat agccgccgaa
    23881 actgcggatc aaccagacta ataagcccgc cagcgcgccg aacatcagcc gaccacgatt
    23941 ggtcgtagaa gcagtaactg ggtcagtcaa aataaagaat gcgccgagca tagtcgcacc
    24001 agacagcaga tgaatttgcg gtgctgccag tgtttctggt gagaacaacc agcccaacgt
    24061 tgcgcataac gccagcgtta ctaagaagct gaggggaata tgccagcgaa tcgctttctg
    24121 ccatagcaac cacacgccac cagccagcca ggcgagattt acccattgcc aaccagcgcc
    24181 cgccagaata ccgctgtaga tcggatattg cataatctgt tcaaccgaat gacctgcgcg
    24241 gacagaggtt ttgaatgtat ccagcggtgt cgcctgacta atgccatcaa tacctaagcg
    24301 tagcgtattc atatcaccac cactggcggt atgaccgctg aaaataacct gtatggcgtc
    24361 gataaaacca gggatgttga ccgcaatttc atgtggcggt aaccagctgg tcatctgcac
    24421 cgggaaggag atcagtaaga ccacataacc aatcattgcc ggattaaacg ggttttgccc
    24481 cagaccgcca tacaactgtt tagcgatgat caccgcaaac accgtaccca gtacgaccat
    24541 ccaccatggc gctagggggg gaatacttac cgccagcaat aagcctgtca gcaatgctga
    24601 gttatctttc aacgttgcgg ctaccgactg cttgcgtagt ttgagtacga gagcttcggc
    24661 taacagagca ctgaccgacg ccaacaggat ctgaacgaga gtaccccaac caaaaaacca
    24721 cagttgcgct gcgattcctg gcacggctgc gagcaacacc aacagcataa tgcgcgatgt
    24781 ctggcgctgg ttatgggtat aaggggagct agctattctg aataccattt agtcctcgtt
    24841 tacaaccttt tgctgggcgg cttttttggc ctgaacgcgg gcaatagccg cggcaactgc
    24901 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
    24961 gcgtgctttg gcgcgggcaa tagcggcttc gacggcagct ttgcgcggat caacctgttc
    25021 ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggc
//
LOCUS       DADMFL010000056        24732 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.056, whole genome shotgun sequence.
ACCESSION   DADMFL010000056 DADMFL010000000
VERSION     DADMFL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.056"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            132..197
                     /locus_tag="JNZ27_002262"
     CDS             132..197
                     /locus_tag="JNZ27_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664585.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            336..947
                     /gene="ruvA"
                     /locus_tag="JNZ27_002263"
     CDS             336..947
                     /gene="ruvA"
                     /locus_tag="JNZ27_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY8664586.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERSLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            956..1966
                     /gene="ruvB"
                     /locus_tag="JNZ27_002264"
     CDS             956..1966
                     /gene="ruvB"
                     /locus_tag="JNZ27_002264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY8664587.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2113..2898)
                     /gene="znuB"
                     /locus_tag="JNZ27_002265"
     CDS             complement(2113..2898)
                     /gene="znuB"
                     /locus_tag="JNZ27_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY8664588.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2895..3650)
                     /gene="znuC"
                     /locus_tag="JNZ27_002266"
     CDS             complement(2895..3650)
                     /gene="znuC"
                     /locus_tag="JNZ27_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY8664589.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3729..4661
                     /gene="znuA"
                     /locus_tag="JNZ27_002267"
     CDS             3729..4661
                     /gene="znuA"
                     /locus_tag="JNZ27_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY8664590.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4677..5999
                     /gene="mepM"
                     /locus_tag="JNZ27_002268"
     CDS             4677..5999
                     /gene="mepM"
                     /locus_tag="JNZ27_002268"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY8664591.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6119..7090
                     /gene="lpxM"
                     /locus_tag="JNZ27_002269"
                     /gene_synonym="msbB"
     CDS             6119..7090
                     /gene="lpxM"
                     /locus_tag="JNZ27_002269"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8664592.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7221..8663)
                     /gene="pyk"
                     /locus_tag="JNZ27_002270"
     CDS             complement(7221..8663)
                     /gene="pyk"
                     /locus_tag="JNZ27_002270"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY8664593.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8791..9660)
                     /locus_tag="JNZ27_002271"
     CDS             complement(8791..9660)
                     /locus_tag="JNZ27_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8664594.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9998..11473
                     /gene="zwf"
                     /locus_tag="JNZ27_002272"
     CDS             9998..11473
                     /gene="zwf"
                     /locus_tag="JNZ27_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY8664595.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11708..13519
                     /gene="edd"
                     /locus_tag="JNZ27_002273"
     CDS             11708..13519
                     /gene="edd"
                     /locus_tag="JNZ27_002273"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY8664596.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            13556..14197
                     /locus_tag="JNZ27_002274"
     CDS             13556..14197
                     /locus_tag="JNZ27_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY8664597.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14252..15430)
                     /gene="purT"
                     /locus_tag="JNZ27_002275"
     CDS             complement(14252..15430)
                     /gene="purT"
                     /locus_tag="JNZ27_002275"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY8664598.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            15564..15854
                     /gene="yebG"
                     /locus_tag="JNZ27_002276"
                     /pseudo
     CDS             15564..15854
                     /gene="yebG"
                     /locus_tag="JNZ27_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            15921..16277
                     /gene="yebF"
                     /locus_tag="JNZ27_002277"
     CDS             15921..16277
                     /gene="yebF"
                     /locus_tag="JNZ27_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY8664599.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16604..17263
                     /locus_tag="JNZ27_002278"
     CDS             16604..17263
                     /locus_tag="JNZ27_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY8664600.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17472..19532
                     /gene="ptrB"
                     /locus_tag="JNZ27_002279"
     CDS             17472..19532
                     /gene="ptrB"
                     /locus_tag="JNZ27_002279"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY8664601.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLLELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(19529..20191)
                     /gene="exoX"
                     /locus_tag="JNZ27_002280"
     CDS             complement(19529..20191)
                     /gene="exoX"
                     /locus_tag="JNZ27_002280"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY8664602.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20215..20871)
                     /locus_tag="JNZ27_002281"
     CDS             complement(20215..20871)
                     /locus_tag="JNZ27_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY8664603.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(20973..21203)
                     /locus_tag="JNZ27_002282"
     CDS             complement(20973..21203)
                     /locus_tag="JNZ27_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY8664604.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21342..21716
                     /gene="yobA"
                     /locus_tag="JNZ27_002283"
     CDS             21342..21716
                     /gene="yobA"
                     /locus_tag="JNZ27_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY8664605.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21720..22592
                     /gene="copD"
                     /locus_tag="JNZ27_002284"
                     /pseudo
     CDS             21720..22592
                     /gene="copD"
                     /locus_tag="JNZ27_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            22605..22946
                     /locus_tag="JNZ27_002285"
     CDS             22605..22946
                     /locus_tag="JNZ27_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY8664606.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(23339..24415)
                     /locus_tag="JNZ27_002286"
     CDS             complement(23339..24415)
                     /locus_tag="JNZ27_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8664607.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSLAGRLYKEICGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(24381..24662)
                     /locus_tag="JNZ27_002287"
     CDS             complement(24381..24662)
                     /locus_tag="JNZ27_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8664608.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         6254 a         6079 c         6263 g         6136 t
ORIGIN      
        1 cacctataca tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca
       61 cgttgtcatt ccattgaaat agatacgaat atacgtaata gcataaaaaa attatttttt
      121 caggagtcgc aatgtatatt tttattacgc atttcttcac tgaatatgta atattcaaat
      181 atttgcttcc aatataacct gttgaataaa ttatactgtg ccatttttca gatcatcggg
      241 acacctcgca agttttcttc atcctacgct ggatatctat ccagcatttt tttatcatac
      301 agcattatct ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca
      361 tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc
      421 atatgccgat gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca
      481 cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagaac
      541 gctcgttgtt caaagagttg atcaaaacca acggcgtcgg cccgaagttg gccctggcga
      601 tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt tgagcgtgaa gaagtggggg
      661 cactggtgaa actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga
      721 aagaccgatt taaaggtttg catggcgatc tctttacgcc agccgccgac ctggtactca
      781 cgtctcctgc cagcccggcg accgacgatg ctgaacaaga agcagttgcc gcgctggtgg
      841 cgctaggcta taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg
      901 ccagcagtga aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat
      961 tgaagcagac cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc
     1021 cattcgccct aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga
     1081 gattttcatc aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg
     1141 tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa
     1201 tttacgcacg acttctggtc cggtgctgga aaaggcgggc gatttggctg cgatgctcac
     1261 taaccttgaa ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt
     1321 tgaagaagtg ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg
     1381 tccggcggca cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac
     1441 gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga
     1501 gttttatcag gtgccggatc tgcaatatat cgtcagtcgc agtgcacgct ttatggggct
     1561 tgagatgagt gatgacggtg cgcaggaagt agctcgtcgc gctcgcggta cgccacgtat
     1621 tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat
     1681 ctcggcagat atcgctgctc aggcgctgga tatgttgaat gtcgatgctg aaggtttcga
     1741 ttatatggac cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg
     1801 tctggataac ctggcggcag ccattggcga agaacgtgaa accattgaag atgtactgga
     1861 accttatttg attcagcaag gctttttgca gcgtacaccg cgcgggcgta tggcgacgac
     1921 gcgggcgtgg aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc
     1981 tcgcgccgca tccgacactg gcagtgtgtc tgatgcgaca ctgttcgcgt cttatcaggc
     2041 cagccagaga tagcgaaccg taggtcggat aaggcgctcg cgccgcatcc gacaaatgtg
     2101 ttcagcgata gattagctgg cctgcttttt catcatactg ataataaata acagtgccgc
     2161 acatagcacc accgacggac ccgccggcgt atcgtaaaac gcggaaaagg ttaaaccgcc
     2221 agttactgcc accatcccca ccaaaacagc gacaccagcc atctgttccg gcgtacgggc
     2281 aaagcgacgc gcagtagctg caggaataat cagcaacgaa gtaataatca acgcaccgac
     2341 aaatttcatc gctacaccaa tcgtcaatgc cgtcaccagc atcaacaaca atttcacttg
     2401 ctgtaatttc acaccatcaa caaacgccag atccgggcta atcgtcatcg acagcaaatt
     2461 gcgccattgc cagaacaaaa tagccaccac gatgaccacg ccaattgcaa tagagatgag
     2521 atcttctggc gtcactgcca gtaaatcacc gaacaggtaa gccatcaaat caacacgaat
     2581 gttagacatc agactaacga ccactaggcc cagcgacagg gcactgtgcg ccataatccc
     2641 taataacgtg tcgatcgcca gctgtggacg cttctccagc cataccagac cgcccgccag
     2701 cagcagcgta acggcaatca ccgcatagaa tggattcacg tccagcaaca aaccaaacgc
     2761 gacaccaagt aatgaggcat gagccagcgt atcaccgaaa taagacatac gacgccagac
     2821 tacaaacgaa cccagcggac ccgcggcaca ggcgagcatg atcccggcta accaaccggg
     2881 aaataataat tcaatcatga gcgatcattt ccccgacgca aaacaattcg tccctgtaaa
     2941 tcgtgacgat gattatgatg atggcgatag atacccagtt gttcagcacc acgaggacca
     3001 aacattgaaa taaactccgg atgcagggaa acaacttccg gtgtgccgga acaacaaatg
     3061 tggtgattca ggcacagcac ttcatcggtt tttgccatca ccagatgcag atcgtgagaa
     3121 accattaaaa cgccacaatc cagttcacga cgcaactggt caataaggtc atataacgcc
     3181 acttggccat ttacatccac gccttgagtg ggttcatcca gcactaataa ttgcgggcaa
     3241 tttaacaatg ctcgcgctaa tagtacacgc tgcgtttcgc caccagagag cttttgcatc
     3301 ggtgcgttaa tcaggtgccc ggcctggaca cgtttcagtg caggcaaaat atcttcttta
     3361 tgtgtaccag ggcgtaagcg taaaaaacgg tttacggtca gtggcaacgt ggtgtcgaga
     3421 tacagcttct gcggtacata gccgatgcgc agttttccgt tgcgcttgat aaccccttca
     3481 tcgggtgtta ccagcccgag cactacccgt accagtgtcg acttacctgc gccatttggc
     3541 ccaagtaaag tcaaaatttt tccaggttta agttccagcg acacatcaga gaggacgcgg
     3601 cgttggccaa aagaaaccga gacattttcc agggaaacca gacttgtcat gttaatttta
     3661 gtcttgcagt agtcatgaaa tgttataata tcacacttct catattcatt acgatgattg
     3721 gtcgcattat gttacataaa aaaacgcttc ttttcgcagc attatccgcc gctctctggg
     3781 gaggtgcaac acaggccgca gatgccgccg ttgtcgcttc gcttaaaccc gttgggttca
     3841 tcgcttctgc cattgctgat ggtgtaacag aaacggaggt tttactgcct gacggcgctt
     3901 cagaacatga ttattcactg cgtccatcgg atgtaaaacg cttacagaac gcggacttag
     3961 tcgtttgggt tggcccggag atggaagcgt ttatgcaaaa accggtaagc aaattaccag
     4021 aagcgaagca ggtaacgatt gcgcagcttg aagatgtgaa accgctactg atgaaaagta
     4081 ttcacggcga tgatgatgat cacgaccacg cggaaaaaag tgacgaagat caccatcacg
     4141 gcgatttcaa catgcatctt tggctttccc cagagatagc gcgggctaca gcggttgcaa
     4201 tccatggaaa attagtggaa cttatgccgc aaagtcgagc caaacttgac gccaacctga
     4261 aggattttga ggcacaattg gcctcaaccg aaacgcaggt tggtaacgag ctcgcgccgc
     4321 tcaaggggaa aggttatttc gtttttcacg atgcttacgg ctatttcgaa aaacagttcg
     4381 gactgacacc gcttggtcat tttaccgtta accctgagat tcaacctggc gcgcagcgtt
     4441 tacatgaaat aagaacacag ttggttgagc aaaaagcaac ctgcgttttt gctgagccac
     4501 agttcaggcc agcggtcgtt gagagcgtcg cacgagggac atccgttcgt atgggaacgt
     4561 tggatcccct ggggacgaat atcaaactgg gtaaaacaag ctattcagaa ttcctgagtc
     4621 aattagccaa ccagtatgcg agctgcctga aaggagatta atgaggaagt gattacgtgc
     4681 aacagatagc ccgctctgtc gccctggcgt ttaataattt accgcgacca caccgcgtta
     4741 tgttggggtc gctcaccgtt cttactctgg ccgtcgctgt ctggcggccc tatgtttatc
     4801 accgtgatgc cacgccaatt gtcaaaacca ttgagctgga acagaacgaa attcgttcgc
     4861 tcttacctga agccagtgag ccgattgatc aagctgcaca agaagatgaa gccattcccc
     4921 aggacgaact ggatgacaaa atcgccggtg aagcgggcgt gcatgaatat gttgtttcca
     4981 ctggcgatac gctaagcagc attctcaatc agtatggtat tgatatgggt gatatcaccc
     5041 aactcgctgc tgccgacaaa gaattgcgta acctgaaaat cggtcaacaa ctctcctgga
     5101 cattaaccgc ggacggcgaa ctgcagcgcc tcacctggga agtgtctcgt cgtgaaaccc
     5161 gaacctatga ccgtactgcc gctaacggtt ttaaaatgac cagcgaaatg cagcaaggag
     5221 agtgggttaa caatctgctg aaaggtaccg tcggagggag ctttgttgcc agcgccagaa
     5281 acgccggttt aaccagcgcc gaagtgagcg cagtgattaa agccatgcag tggcaaatgg
     5341 atttccgcaa actgaaaaaa ggcgatgaat ttgcggtatt aatgtctcga gaaatgcttg
     5401 atggtaaacg tgagcaaagc cagctgctgg gcgtacgttt gcgttcagaa ggtaaagatt
     5461 attacgcaat ccgcgctgag gatggcaaat tctacgatcg taacggtact ggtctggcga
     5521 aaggattctt gcgattcccg acggcgaaac agttccgtat ctcgtctaac tttaacccgc
     5581 gtcgaactaa tccggtgaca ggtcgcgttg caccacacag aggtgttgat ttcgccatgc
     5641 cacaaggtac gccagtgctt tcagtgggtg acggtgaagt ggtggttgcc aaacgcagtg
     5701 gcgcagcagg ttattatgtg gctattcgtc atggtcgcag ctacaccacg cgttatatgc
     5761 acttgcgcaa gattctggtg aaaccgggac agaaggtgaa acgtggcgac cgtatcgcgc
     5821 tttccggtaa taccggacgt tcaactgggc cgcatctgca ctatgaagta tggataaacc
     5881 agcaggccgt aaacccgctg acggcaaaac tgccgcgtac cgaagggctg accggctccg
     5941 atcgtcgcga attcctggcg caggccaaag agattgtgcc gcagctacgg tttgattaat
     6001 taacatccat tcgcagccgg tacgcagtca gtaccggctt tttttatttg gtgcggggca
     6061 agttgcgccg ctacactatc accagattga tttttgcctt atccgaaact ggaaaagcat
     6121 ggaaacgaaa aaaaataata gcgaatacat tcctgagttt gataaatcct ttcgccaccc
     6181 gcgctactgg ggagcatggc tgggtgtagc agcgatggcg ggtatcgctt taacgccgcc
     6241 aaagttccgt gatcccattc tggcacggct gggacgtttt gccggacgac tgggaaaaag
     6301 ctcacgccgt cgtgcgttaa tcaatctgtc gctctgcttt ccagaacgta gtgaagctga
     6361 acgcgaagcg attgttgatg agatgtttgc caccgcgccg caagcgatgg caatgatggc
     6421 tgagttggca atacgcgggc cggagaaaat tcagccgcgc gttggctggc aagggctgga
     6481 gatcatcgaa gagatgcggc gtaataacga gaaagttatc tttctggtgc cgcacggttg
     6541 ggccgtcgat attcctgcca tgctgatggc ctcgcaaggg cagaaaatgg cagcgatgtt
     6601 ccataatcag ggcaacccgg tttttgatta tgtctggaac acggtgcgtc gtcgctttgg
     6661 cggtcgtctg catgcgagaa atgacggtat taaaccattc atccagtcgg tacgtcaggg
     6721 gtactgggga tattatttac ccgatcagga tcatggccca gagcacagcg aatttgtgga
     6781 tttctttgcc acctataaag cgacgttgcc cgcgattggt cgtttgatga aagtatgccg
     6841 tgcgcgcgtt gtaccgctgt ttccgattta tgatggcaag acgcatcgtc tgacgattca
     6901 ggtgcgccca ccgatggatg atctgttaga ggcggatgat catacgattg agcggcggat
     6961 gaatgaagaa gtcgagattt ttgttggtcc gcgaccagaa caatacacct ggatactaaa
     7021 attgctgaaa actcgcaaac cgggcgaaat ccagccgtat aagcgcaaag atctttatcc
     7081 catcaaataa aaaagcctct cgcgaggaga ggccttcgcc tgatgataag ttcaagtttg
     7141 cttcagaata ttcgaaatct gttgaactat cattgaacta taggccggat gtggcgtttt
     7201 cgccgcatcc ggcaacgtac ttactctacc gttaaaatac gcgtggtatt agtagaaccc
     7261 acggtactca tcacgtcgcc ctgggtgaca atcaccaggt caccagacat caagtaacct
     7321 ttatcgcgca gcagattaac cgcttcgctg gcagctgcta cgccgtcatt agcgctatca
     7381 aagtgcaccg gcgtaacgcc acgatagaga gcagtcaggt tcagcgtacg ttcatggcgc
     7441 gacatggcga aaattggtag accagagctg atacgggagg tcatcagcgc ggtacgaccc
     7501 gattcggtca tggtaatgat cgccgtaacg cccttcaggt ggttagctgc gtacattgcc
     7561 gacatggcaa tagcttcttc cacattgtcg aactgaacgt ccagacggtg tttagaaacg
     7621 ttgatgctcg ggattttttc cgcacccagg caaacgcgcg ccatggctgc aacagtttct
     7681 gacggatact gcccagcggc agtttctgca gacagcatca cagcgtcagt accatccaga
     7741 acggcgtttg ctacgtccat gacttctgca cgcgtcggca tcgggttagt aatcattgac
     7801 tccatcatct gggtcgccgt gattaccgct cggtttagct gacgcgcacg acggatcaac
     7861 gctttctgaa tgccgaccag ttccgggtcg ccaatttcca caccgaggtc gccacgtgca
     7921 accattacca cgtcagaggc gaggatgatg tcatccattg catcctggct gcaaacggct
     7981 tccgcacgtt caaccttggc aacaattttc gcatcacatc ctgcatcgcg tgccaggcga
     8041 cgggcatagt tcaggtcttc gccacagcgt gggaaggaga cagccaggta atctacgcca
     8101 atcaacgccg cagtcttaat gtctgctttg tctttttcgg tcagcgcttc agccgacaaa
     8161 ccgccgccaa gtttgttgat acctttattg ttggagaggg gaccaccgac ggtgacttcg
     8221 gtgaacactt tcatgccctg aacttccagt acttttaact ggacgcgacc atcgtccagc
     8281 agcaggatgt caccaggcac gacgtcagca ggcaggcctt tgtagtcgat accgactttt
     8341 tctttgtcgc cttcaccttt acccaggttg gcgtcgagca ggaatttatc cccaatattg
     8401 aggaaaactt tgccttcttt aaaggtggat acacggattt tgggcccctg gaggtcaccc
     8461 agaatagcca catgacgccc cagttttgcg gcaatctcac gaactttatc cgcgtgcatt
     8521 ttgtgatctt caggcgagcc gtgagaaaag ttcatacgta caacgttggc acccgccgcg
     8581 ataacttttt caagattatt atcgcgatct gttgctgggc ctaacgtggt aacgattttt
     8641 gttctgcgaa gccttttgga catgtaatac tccgttgact gaaacaacca ggtgttgctt
     8701 gaacatgaaa tccgaatgaa ataacgccgc gatggcgata aattaatctc attcggatgt
     8761 cgtataagat taggacagtg acagtcgctt ttagcgatcg tcacttaaat taagtaactg
     8821 cttatcaaaa cgcgattcct tcagcgcttc cttgacccgc ttcaagttat ctctgaattt
     8881 tgcaccgcgt cgcaaagtaa atcctgtcgc cagcacatct atcacggtca gctgtgcaag
     8941 tcgagaaacc atgggcatat aaatgtcagt atcttccggt acgtcgaggg taattgccag
     9001 cgttgcttcc cgggcgagcg gggtacccgc agaggtgagg gcaatcacca tggcgtcgtt
     9061 ttcgcgtgcc agctgcgcca gctcgaccag attttttgtt cttcctgtgt gagaaatcag
     9121 caccaccacg tctccgtcgc tacaattcat acaactcatg cgttgcagca cgatatcatc
     9181 ggagtacacc accggaacat taaaacgaaa gaacttattc atcgcatcgt gggcaacggc
     9241 ggcagaagag cctaatccga aaaaggcgat tttttttgcc tgagtgagca agtcgacggc
     9301 gcggttgatg gcagatttat ccagtgaatg acggacatga tcgagcgttg ccattgcgga
     9361 ctcaaatatt ttccccgtgt atgattcaac gctgtcatct tcattgacgt tgcgattaac
     9421 atagggagtg ccattcgcca gactttgtgc cagatgaagt ttaaaatcag gaaaaccgcg
     9481 cgtgtccatg ctgcgacaga aacgattcac cgtcggttcg ctaacattgg cttccagcgc
     9541 cagagcagca atactcgaat ggatcgcgtt atcgggcgaa gccagaatga cctcggcaac
     9601 tttgcgctct gatttgctca aatgttccag ctgagactgg attttttcca gcatattcat
     9661 gatgtaaaga gactcacggg taatgacgat ttccgcactg aaagaaatcg aaatgcagtt
     9721 ttgtcagata ttacgcctgt gtgccgtgtt aatgacaaaa gcagataaaa aagttgttat
     9781 tttttttcat aacatgatca gtgtcagatt tttaccgaat ggaaaacgat gattttttta
     9841 tcagttttgc cgcactttgc gcgcttttcc cgtaatcgca cgggtggata agcgtttaca
     9901 gttttcgcaa gctcggaaaa gcagtacagt gcaccgtaag aaaattacaa gtataccctg
     9961 gcttaagtac cgggttagtt aacttaagga gaatgacatg gcggtaacgc aaacagccca
    10021 ggcctgtgac ctggtcattt tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc
    10081 ttccctgtat caactggaaa aagccggtca gctcaacccg gacacccgga ttatcggcgt
    10141 agggcgtgct gactgggata aagcggcata taccaaagtt gtccgcgagg cgctcgaaac
    10201 tttcatgaaa gaaaccattg atgaaggttt atgggacacc ctgagtgcac gtctggattt
    10261 ttgtaatctc gatgtcaatg acactgctgc attcaaccgt ctcggcgcga tgctggatca
    10321 aaaaaatcgt atcactatta actactttgc catgccgccc agcacttttg gcgcaatttg
    10381 caaagggctt ggcgaggcaa aactgaatgc taaaccggca cgcgtagtca tggagaaacc
    10441 gctggggacg tcgctggcga cctcgcagga aatcaatgat caggttggcg aatacttcga
    10501 ggagtgccag gtttaccgta tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt
    10561 ggcgctgcgt tttgctaact ccctgtttgt gaataactgg gacaatcgca ccattgatca
    10621 tgttgagatt accgtggcag aagaagtggg gatcgaaggg cgctggggct attttgataa
    10681 agccggtcag atgcgtgaca tgattcagaa ccacctgcta caaattctct gcatgattgc
    10741 gatgtctccg ccatctgacc tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact
    10801 gaagtccctg cgccgcatcg accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata
    10861 tactgcgggc ttcgcccagg gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa
    10921 caaaagcagc aatacagaaa ccttcgtggc gatccgcgtc gacattgata actggcgctg
    10981 ggccggtgtg ccattctacc tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt
    11041 cgtggtctat ttcaaaacac ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc
    11101 gcagaataaa ctgactatcc gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa
    11161 taaagttcct ggccttgacc acaaacataa cctgcaaatc accaagctgg atctgagcta
    11221 ttcagaaacc tttaatcaga cgcatctggc ggatgcctat gaacgtttgc tgctggaaac
    11281 catgcgtggt attcaggcac tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg
    11341 ggtagactcc attactgagg cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc
    11401 cggaacctgg ggacccgttg cctcggtggc gatgattacc cgtgatggtc gttcctggaa
    11461 tgagtttgag taatatcagc gcttatcctt tatggttatt ttaccggtaa catgatcttg
    11521 cacagattgt agaacaattt ttacactttc aggcctcgtg cggattcacc cacgaggctt
    11581 tttttattac actgactgaa acgtttttgc cctatgagct ccggttacag gcgtttcact
    11641 cataaatcct ctgaatgaaa cgcgttgtga atcatcctgc tctgacaact caatttcagg
    11701 agcctttatg aatccacaat tgttacgcgt aacaaatcga atcattgaac gttcgcgcga
    11761 gactcgctct gcttatctcg cccggataga acaagcgaaa acgtcgaccg ttcatcgttc
    11821 gcagttggca tgcggtaacc tggcacacgg tttcgctgcc tgccagccag aagacaaagc
    11881 ctctttgaaa agcatgttgc gtaacaatat cgccatcatc acctcctata acgacatgct
    11941 ctccgcgcac cagccttatg aacactatcc agaaatcatt cgtaaagccc tgcatgaagc
    12001 gaatgcggtt ggtcaggttg cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg
    12061 gcaggatgga atggaattgt cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt
    12121 ggggctgtcc cataacatgt ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt
    12181 cccgggtctg acgatggcag ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc
    12241 tggaccgatg gcaagcggtt tgccaaataa agaaaaagtg cgtattcgcc agctttatgc
    12301 cgaaggtaaa gtggaccgca tggccttact ggagtcagaa gccgcgtctt accatgcgcc
    12361 gggaacatgt actttctacg gtactgccaa caccaaccag atggtggtgg aatttatggg
    12421 gatgcagttg ccaggctctt cttttgttca tccggattct ccgctgcgcg atgctttgac
    12481 cgctgccgct gcgcgtcagg ttacacgcat gaccggtaat ggtaatgaat ggatgccgat
    12541 cggtaagatg atcgatgaga aagtggtggt gaacggtatc gttgcactgt tggcgaccgg
    12601 tggttccact aaccacacca tgcacctggt ggcgatggcg cgcgcggccg gtattcagat
    12661 taactgggat gacttctctg acctttctga tgttgtaccg ctgatggcac gtctctaccc
    12721 gaacggtccg gccgatatta accacttcca ggcggcaggt ggcgtaccgg ttctggtgcg
    12781 tgaactgctc aaagcaggcc tgctgcatga agatgtcaat acggtggcag gctttggtct
    12841 gtttcgttat acccttgaac catggctgaa taatggtgaa ctggactggc gggaaggggc
    12901 ggaaaaatca ctcgacagca atgtgatcgc ttccttcgaa caacctttct ctcatcatgg
    12961 tgggacaaaa gtgttaagcg gtaacctggg ccgtgcggtt atgaaaacct ctgccgtgcc
    13021 ggttgagaac caggtgattg aagcgccagc ggttgttttt gaaagccagc atgacgttat
    13081 gccggccttt gaagcgggtt tgctggaccg cgattgtgtc gttgttgtcc gtcatcaggg
    13141 gccaaaagcg aacggaatgc cagaattaca taaactcatg ccgccacttg gtgtattatt
    13201 ggaccggtgt ttcaaaattg cgttagttac cgatggacga ctctccggcg cttcaggtaa
    13261 agtgccgtca gctatccacg taacaccaga agcctacgat ggcgggctgc tggcaaaagt
    13321 gcgcgacggg gacatcattc gtgtgaatgg acagacaggc gaactgacac tgctggtaga
    13381 cgaagcggaa ctggctgctc gcgaaccgca cattcctgac ctgagcgcgt cacgcgtgag
    13441 aacaggacgt gaattattca gcgccttgcg tgaaaaactg tccggtgccg aacagggcgc
    13501 aacctgtatc actttttaag acgacaaatt tgtaatcagg cgagagaaaa ctctgatgaa
    13561 aaactggaaa acaagtgcag aatcaatcct gaccaccggc ccggttgtac cggttatcgt
    13621 ggtaaaaaaa ctggaacacg cggtgccgat ggcaaaagcg ttggttgctg gtggggtgcg
    13681 cgttctggaa gtgactctgc gtaccgagtg tgcagttgac gctatccgtg ctatcgccaa
    13741 agaagtgcct gaagcgattg tgggtgccgg tacggtgctg aatccacagc agctggcaga
    13801 agtcactgaa gcgggtgcac agttcgcaat tagcccgggt ctgaccgagc cgctgctgaa
    13861 agctgctacc gaagggacta ttcctctgat tccggggatc agcactgttt ccgaactgat
    13921 gctgggtatg gactacggtt tgaaagagtt caaattcttc ccggctgaag ctaacggcgg
    13981 cgtgaaagcc ctgcaggcga tcgcgggtcc gttctcccag gtccgtttct gcccgacggg
    14041 tggtatttct ccggctaact accgtgacta cctggcactg aaaagcgtgc tgtgcatcgg
    14101 tggttcctgg ctggttccgg cagatgcgct ggaagcgggc gattacgacc gcattactaa
    14161 gctggcgcgt gaagctgtag aaggcgctaa gctgtaaaag tcaaaatgcc cgatctagga
    14221 tcgggcattt ttgtagcgtt ttttgccggg tttaaccctg tacttttacc tgtccggcgg
    14281 cgtgcttcgc gcgttcaatg gcgtcaacaa cactctctga agtagccagt accaccccca
    14341 gacgacggct gccatcaatt tccggcttac caaataaacg aatctgcaaa tctgcgccta
    14401 cggcattctg cacattatca aacgtgacat tctgactggt cagttgtggc agaataacgg
    14461 cagaagctgc aggaccatac tgacggatcc cgccaacagg aagtccgagg aaggcacgta
    14521 catgcagggc aaactctgag agatcttgag aaattaacgt caccatcccg gtatcatgtg
    14581 gacgagggga gacctcactg aaaatcacct catcaccaca gacaaacagc tcgacaccaa
    14641 acagaccata accgcccagt gccagcacca ctttacgggc aatctcctgc gcacgttcaa
    14701 gggcaagcgg gctcatttgc tgtggttgcc aggattcacg gtagtcgcca tcttcctggc
    14761 gatgacctac tggtgcacag aaatggacgc catccaccgc gctgacggtt agcagggtaa
    14821 tttcgaagtc aaacttaacg acgccttcaa caattacgcg gcccgctccg gcgcgaccgc
    14881 cttgctgagc gtattcccat gcctgagcaa gttgctctgc agagcgaata aacgtctgcc
    14941 ccttgccgga agagctcatc accggtttta caatgcaggg atagccaatg gcggcaaccg
    15001 cctcgcggaa aaggcttttg ctatcggcaa aacgataagt ggaagtgggc agctgcagct
    15061 cttctgccgc caggcgacgg ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc
    15121 aggggacaac attcagtcct tcctcttcaa gttggatcag catatcggtg gcaatagctt
    15181 cgatctccgg cacgatataa tgtggttttt ccagttcaac cacacggcgt aatgcatcac
    15241 catcaagcat attaatgaca tgggagcgat gcgcgacatg catggctggt gcgtcggcat
    15301 agcgatcgac ggcaatcact tctacgccga gacgctgaca ctcgattgcc acttctttac
    15361 ccagttcacc ggagcctaat aacatcacgc gagttgctgc cggacgcagc gcagtgccta
    15421 ataacgtcat aacgaatatc ccctgattaa ttccgcgcgc agtataaacg aaaacgtttg
    15481 cgtgtgtctt tattgctgat gttgatttct caaccgaaaa gaaatatact gtataaaatc
    15541 acagttatta tgagaggctg caaatggcgg ttgaagttaa atacgtagtc attcgtgagg
    15601 gcgaagagaa aatgtcgttt accagcaaaa aggaagccga tgcttatgac aaaatgcttg
    15661 atacggcgga tctgcttgac acctagctga caaattctcc agtgcaaatg gaagacgagc
    15721 aacgtgaagc cctttcgcta tggctggcag aacaaaaaga tgtgctgagc accattctga
    15781 aaaccggcaa attaccgtcc ccactggtcg tcggtgctga atccgaagag gaagatgcct
    15841 cacacgcggc ctgaagcaac ttgcgctcct gacaatttgt accatgcttt ctcctgaatc
    15901 attgtgctat ggagaaaaac atgaaaaaaa gaggggcgtt tttagggcgg ttgttggttt
    15961 ctgcctgcgc atcagttttc gctgccaata atgaaaccag caagtcggtc actttcccaa
    16021 agtgtgaagg tctggatgct accggaattg ccgcgagcgt aaaacgtgat tatcaacaaa
    16081 atcgcgtggc gcgctgggca gatgatcaaa aaattgtcgg tcaggccgat cccgtggcct
    16141 gggtcagttt gcaggacatt cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc
    16201 gtggtaaaag tgccgatatt cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg
    16261 aatatcagcg gcgttaatgg cttctgataa ttcgattgac cgttggcgga tgcggcgcga
    16321 gtgccttgtc cgtcccagaa cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg
    16381 catcgggcaa tcagcatcaa acaatgccac tcaaaacgac aaactacttt cacctaaaat
    16441 acccttatca cctgatatac agtaaatacc ttacctggcg cgaatttctt tgaataacgg
    16501 cctaccgccc cgcttatttg cgcatctttt gtctcgatat acattttctg acatcctcat
    16561 ccgctatgct acaggcacta aaaatggtta ttgaggagca aatatggcta actggttaaa
    16621 tcaactgcaa tccctgcttg ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg
    16681 attgggcaaa ctgttagtgc caggcgcatt aggcggactg gctgggctgc tggtcgcaaa
    16741 taaatcagca cgtaaacttc ttacgaaata tggcaccaac gcgttactgg ttggcggcgg
    16801 agcggtagcg ggtacggtgc tgtggaataa atacaaagat aaaattcgcg cggcgcatca
    16861 ggacgaaccg cagtttggcg cgcaaagtac gccgctggat gagcgtacgg aacgtttgat
    16921 ccttgcgctg gtctttgccg ctaaaagtga tggtcatatt gatgccaatg aacgtgcggc
    16981 aatcgaccag caattgcgtg aagccggcgt ggaagagcag gggcgtgtac tcattgagca
    17041 ggcaatcgaa caaccgctgg atccacaacg cctggctacc ggggtccgca atgaagaaga
    17101 ggcgctggaa atctatttcc tgagctgcgc ggctattgat attgaccatt ttatggaacg
    17161 cagttacctg aatgcactgg gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat
    17221 tgaacgcgat ctcgaacagc aaaaacgcac tttggcggaa taattccgct ctctggatag
    17281 cctgaagttg actatttcgg gctatccaga accgcacagc catttgaaca ggctgatttt
    17341 ctctgtctgc ataacattcg atatccgcgc cattacctgt catcatctaa gcaatgacga
    17401 ccctgtttcg cttgcatccc cggtgagttt tgccaccctt ataagatgtt tcaaccagaa
    17461 agaacaataa catgctacca aaagccgccc gcattcccca cgccatgacg cttcatggcg
    17521 atacgcgcat cgataattac tactggctgc gggacgatac gcgttctcag ccagaagtcc
    17581 tggactacct gcaacaagaa aatagttacg gtcatcgggt gatggcctca caacaagcct
    17641 tgcaggatcg catcttaaag gaaatcatcg accgcattcc gcaaagagaa gtttctgcgc
    17701 cctacataaa aaatggctac cgctatcggc atatttatga accaggctgt gaatatgcta
    17761 tctaccagcg tcaatcggca ttcagcgaag agtgggatga gtgggaaaca ttgctcgatg
    17821 ccaacaagcg cgcagctcat agtgagtttt attcgatggg cggaatggcg attacgcccg
    17881 ataacaccat tatggcgctg gcagaagatt ttctttcccg acgccagtac ggcattcgtt
    17941 ttcgtaatct ggaaacaggt aactggtacc cggaactgct ggataacgtt gaacccagct
    18001 ttgtctgggc aaatgactcc tggactttct actatgttcg caagcatccg gtgacgctgc
    18061 tgccttatca ggtctggcgt cacgccatcg gtacgccagc atcgcaagat acactgatct
    18121 acgaagaaaa agacgatacc tattatgtca gcctgcataa aacgacgtcg aagcactatg
    18181 tcgtcattca tctggccagc gccaccacca gtgaagttcg cctgctggac gcggaaatgg
    18241 ccgatgccga gccgtttgtt tttctgccgc gccgcaaaga tcacgaatac agccttgatc
    18301 actaccagca tcgtttttat ctgcgttcca atcgccacgg caaaaacttt ggcttatacc
    18361 gtacccgtat gcgtgatgag caacagtggg aagagttaat tccgccacgt gaaaacatca
    18421 tgctggaagg gtttacgctg tttaccgact ggctggtggt tgaagagcgt cagcgcgggt
    18481 taaccagttt gcgccaaatt aaccgcaaga cccgggaagt cattggcatt gcctttgatg
    18541 atccggccta tgtgacctgg attgcctaca atccagaacc tgaaaccgcg cgattgcgtt
    18601 atggttattc ttccatgacc acaccagaca ctttgcttga actggatatg gataccggtg
    18661 agcgtcgtgt attaaaacaa acagaagtgc ccggttttga tgcggcgaat taccgcagtg
    18721 aacacctgtg gatagtcgcc cgtgatggcg tcgaagttcc ggtttcgctg gtctatcatc
    18781 gcaaacattt tcgcaaagga cacaacccgt tgctggtgta tggctatggt tcttacggcg
    18841 caagtattga tgccgatttc agttttagcc gcttgagttt gttagatcgt ggctttgtct
    18901 acgccattgt ccatgttcgc ggcggtggtg agctggggca acaatggtac gaagacggta
    18961 aatttctgaa gaagaaaaat acgtttaatg attatcttga tgcctgcgat gcattgttaa
    19021 aactgggcta tggctctcct tcgctctgtt atgcgatggg cggaagtgcg gggggcatgt
    19081 tgatgggcgt tgcaattaat caacgcccgg aattattcca cggcgttatc gcccaggtac
    19141 cgtttgttga tgttgtaaca acgatgcttg atgaatcaat tcctcttacc actggtgagt
    19201 ttgaagagtg gggaaatccg caggatccgc aatattacga gtacatgaaa agctacagcc
    19261 cgtatgacaa cgtcaccgca caggcttatc cgcatttact ggtaacgacc ggtttacacg
    19321 attctcaggt gcaatattgg gaaccggcaa aatgggtcgc taaattgcgc gagctgaaaa
    19381 ccgatgacca tcttttattg ctctgtaccg acatggactc aggccatggc ggtaaatctg
    19441 gccgctttaa atcgtacgaa ggcgtagcga tggaatatgc ttttctggtc gcgctggcgc
    19501 agggaacatt acccactacg cctgcggatt aagtattttc cagataatgt ttcagtgtta
    19561 aacgcagctc cgggctcatg ctgtccaggt tattaaataa ccagcgcaga tagcccggat
    19621 cgcgttcggc aacgtcggaa accgctttgc cacggtattt gccaaaggtg aaggtcgtca
    19681 tcaacgacgg acgtccggtg atatcggcca tctgttctgc cgtccagccg gaggtgttca
    19741 taatatcgat aagcaacgcg gcggtgatat aacagtcata caacgcgcgg tgatgatgca
    19801 ggcccggcgg tgtctgtaca ttgagcttgc gtgttttata taacgccata ttgctgtact
    19861 tgagccctgg ccacaaacga cgggccagtt tcatagtgca aatccactca ccgggcatct
    19921 caggcagtac gcggcggtca aagctggcgt tgtgcgcgac ataccattca ctaccgtaat
    19981 agtgtgggat cacatcttca atccacggtt tatcggcgac catggcttcg gtgatgcgat
    20041 gaatcgccat cgcttgtgga ctaataggac gatcggggcg caccaggagg ctcatggggt
    20101 tgacgatttt tccgtcaatg acatcaacag aggcaatctc aacgatccct ccctgcaaac
    20161 cgcaggtttc tgtatcgata atgcgcaaca tggcatgctc caggccgaaa aagcctagcg
    20221 taatggaatg atatcgcctt gccaaccctg tgatgaacgc tgaccgacta acaataatga
    20281 accacgatcg gcgcgaacga tgagacgacc atattcatcc catagtgcac tgttaccacg
    20341 ggcatttgcc atcagcacgg caatcgagta ctgatgtgaa aaaaattgta agcgacgggc
    20401 cgacgccagc aggtcgggtt ctcccagaca ttgccctgtc gtaaacagtg aacaggtcgg
    20461 gtccatatcc ataccttgcg gttgttcatc gaccaccgtg atggttctgg aacgacggcc
    20521 cagacatgca ccatgactct gatggtagtt cccaggcgtt tttcgccacg gtgcgaacac
    20581 cgcaattcca cgaataaagc gatcgttata ttcaacggga aggccggcaa tgatggtcat
    20641 tcgccaggtc gttgcggcat aacaaagcgg gtctaacagt gaaagatcgg gtggggcagg
    20701 aagggcacgt cgcgaataat cacaccccag taaagaaagc gatggaaaaa ctaacagctg
    20761 acattgttgc ctggcggcgg ccctcacaaa ctccagatga tgagctacct gttcggtgag
    20821 tgaggtttta cgaggctcat attgcgctgc tgcaactttc cagaacgaca taatgacttc
    20881 cttttcaaaa tgactgcaaa ttatgggggc ggtttagaaa atactcataa tttagcaggc
    20941 gttatgtaag aaagtgtaac tctgtataag ttttatttaa gtttgggctc gtaaggtaaa
    21001 cgtgacagat tgaccgaagc caaacggtgg gcaataagcc gctcgcgaaa ccagctgcgc
    21061 aaatgttctg gctgttcacg ttcaaccgct tcagcgatca ccggcatatt gtagcgttct
    21121 ttaaaagcca ccccggccgc cgccaaatcg acattcactt tatccatttc tgtttgatcc
    21181 agtttagcca gattcttcag cattcttaat ctcctcatca ttcgcgggca aaagttacta
    21241 agagtgagcc tggatggcaa gccggaattg atgatcctcg actcagacat tagacaaggt
    21301 tatgctctgt aaaaaagtta ttacaaaaag gaatagataa tatggcttca actgcacgct
    21361 cccttcgcta cgcgctgaca atcctgacca cttcgttggt gacaccttcc gtctgggcac
    21421 atgcgcattt aacgcatcag tatcctgcgg caaacgcgca agtgacagct gccccgcagg
    21481 caatcacttt aaacttctcg gaaggcgttg aaacaggatt cagtggtgca aaaatcacgg
    21541 ggccaaaaaa cgaaaatatt aaaacattac cagcgaagcg gaatgagcag gaccaaaagc
    21601 aactgattgt tccactggcc gattcgctga aacccggaac ctacaccgtt gactggcatg
    21661 ttgtttcggt ggacgggcat aaaacgaaag gacactacac ctttagcgtg aaataaatca
    21721 tgctggcgtt tacctggatc gcgctgcgat ttatccattt cacctcgttg atgctggtct
    21781 ttggctttgc aatgtatggt gcctggctgg cccccttaat gattcgtcgc ttgctgacga
    21841 agcgttctct gcgtttacaa cagcacgctg ccgtctggag tttgatcagt gccactgcaa
    21901 tgctcgccgt tcagggcggg ttgatgggaa cgggatggac agatgtattt tcgccaaaca
    21961 tttggcaggc tgttttgcaa acgcagtttg gtggcgtctg gttatggcaa attgttctcg
    22021 ccctcgtaac gttgatcgtc gcccttatgc aaccgcggaa tatgccacgt ttgttgttta
    22081 tgctcactac cgcgcaattt attctgctgg cgggagtggg gcatgcgacg ctgaatgaag
    22141 gggtaacagc gaaaatccat cagactaatc acgcgattca cctgatttgt gcggcggcgt
    22201 ggtttggggg attactgccg gtgctctggt gtatgcaact catcaaaggc cgttggcgac
    22261 accaggctat tcaggcgctg atgcgttttt cctggtgcgg gcattttgcg gtgatcggcg
    22321 tactggcaag cggcgtgctt aatgcattgt tgattaccgg atttcccccc acgcttacga
    22381 cttactaggg ccaactctta ttgctcaaag ccatcctggt gatgattatg gtggtcattg
    22441 cgctggctaa tcgttatgtt cttgtaccgc gcatgaggca ggatgaagat cgtgcggcac
    22501 cctggtttgt gtggatgact aaactcgaat gggcgatagg tgcggtagtg ctggtgatca
    22561 tcagcctgct tgcgactctc gaaccttttt gatggattac gaaaatgatg aaaaaaagta
    22621 ttctggcgtt tctgttactc accagttctg cagcggcgct ggcggctcca caggtgataa
    22681 ccgtgagtcg ttttgaagtg ggtaaagaca aatgggcgtt taatcgcgaa gaggtaatgc
    22741 tgacttgccg accgggtaat gctttgtatg tcatcaaccc aagtaccctc gtgcagtatc
    22801 ctttaaacga tatcgcacaa aaggaagttg ccagtgggaa gaccaatgcc caacccattt
    22861 cggtgattca gattgatgat cctaacaatc ccggcgaaaa aatgagtctg gcaccgttta
    22921 tagaacgagc tgaaaaactc tgttaattac ctaaaatagc cttttgattt ccaataaaaa
    22981 aaacgcctca gttctttcac cagaacgggc ggtttttaac atttaagctg atgaccaccg
    23041 cgctttttat tgaccatttt gcacgcaaac tggaaaacct ggcgtcgtca tctattctta
    23101 aagggcaagg caactaagcc tgcattaatg ccaactttta gcgcacggct ctctcccaag
    23161 agccatttcc ctggaccgaa tacaggaatc gtattcggtc tttttttaat tgtatttaaa
    23221 atcaattagt tgcaaacgtc tccccgaaat tccccgaaat ttactcgaat ttctgtattc
    23281 cggtcttttt tggttatatc acaaccaaaa tacatttaac aatccattta cgttaaaatc
    23341 agagcagtaa gtacgttttt tctctctcat caagatacat ttttgttgtc ttctccgatg
    23401 tgtggccaag tagacgctga gcaaattctt ctccacatat ttctttgtac aatcgtccag
    23461 ccaggcttct gatttcgtga aaagttggtg ggttttcact gaacaggata cctgttaatt
    23521 ttctggctgc gacaaatttc tttgtcaggc cgtccgggtg aatgctgccg tcagggctgt
    23581 tttttctaat cccggcactg attagataat cccccctact taccatgcgg cactgttcaa
    23641 ctactgcacc aagtcgtaaa ccagcgacag gaaggctgag tgacaggggg atagcaatca
    23701 tcattcctgt cttaatttgc ctgatgtgga gacgatcatc ataaatatca ctgaaccgca
    23761 tattcgttat gtcttcgcga cgttgtcctg ttacaagggc taagtccata gctaatggga
    23821 accatgccgg aagttgatct gctgcctccc tgatgcagtt gtatgtcttt agtttcagcc
    23881 gttctcttgt aactactatt ttcggtgctc ttgttggcgt tactggattt tgagatatac
    23941 gtccttcaac aatggcttcg cggaacatat cagataacac agaacgcatt gatcctgcca
    24001 tcgtgttttt ccctccttca atccacaaat caagaaactc ggcgatatgg cgagtggtta
    24061 tttctgtcag taaaacccct cctaattttt cttttattgt caccagttga tttacccgag
    24121 ttttataagt atttctggac gcttttctcc ttataagaat cgttttgtaa cgttcaatcc
    24181 agtctgccat agtaaatgag tcgacccctt taagcttttc aattagggca gcaggagagt
    24241 agtttttgta tatataatga tttgcttcaa ttgcctgcgc tactgcatct cttcttgaaa
    24301 ttttacctag tgtaaattct tctttcgtca gagggttgcg ccagtaatat gctttgtctc
    24361 tccttcgata tgttaagttt ctaggcaaat tgggatcgta ttttttccgc tgcatgtttt
    24421 aacttctcca ttaacggact gtctctccct tgtcgcccat taggctgatg gtgtgttata
    24481 tcggtatcaa ccttatttgg gttgatatag aaagcctccg gaaccaccct gtaactcctc
    24541 ccgtgtagtt caggtgcagg ataaatgttt ccattccttg cccatcgtct cagcgttgat
    24601 attgatggtg ggttatccgg atatctgagt tttccccacg ttttgagtgt cacaagattc
    24661 attgccatac ctctcacgat atgaccgcca gtaaatatac agaatactgg cgggtgtggt
    24721 tgatttttaa ta
//
LOCUS       DADMFL010000057        24492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.057, whole genome shotgun sequence.
ACCESSION   DADMFL010000057 DADMFL010000000
VERSION     DADMFL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.057"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..489)
                     /locus_tag="JNZ27_002288"
     CDS             complement(<1..489)
                     /locus_tag="JNZ27_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8664609.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLE"
     gene            762..950
                     /locus_tag="JNZ27_002289"
     CDS             762..950
                     /locus_tag="JNZ27_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664610.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            962..1672
                     /gene="bcsQ"
                     /locus_tag="JNZ27_002290"
     CDS             962..1672
                     /gene="bcsQ"
                     /locus_tag="JNZ27_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY8664611.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1669..4276
                     /gene="bcsA"
                     /locus_tag="JNZ27_002291"
                     /pseudo
     CDS             1669..4276
                     /gene="bcsA"
                     /locus_tag="JNZ27_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4287..6662
                     /gene="bcsB"
                     /locus_tag="JNZ27_002292"
     CDS             4287..6662
                     /gene="bcsB"
                     /locus_tag="JNZ27_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY8664612.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAVLAVLAVLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6669..7775
                     /gene="bcsZ"
                     /locus_tag="JNZ27_002293"
     CDS             6669..7775
                     /gene="bcsZ"
                     /locus_tag="JNZ27_002293"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY8664613.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7834..8010
                     /locus_tag="JNZ27_002294"
                     /pseudo
     CDS             7834..8010
                     /locus_tag="JNZ27_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8338..10086
                     /gene="bcsC"
                     /locus_tag="JNZ27_002295"
                     /pseudo
     CDS             8338..10086
                     /gene="bcsC"
                     /locus_tag="JNZ27_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10168..12156
                     /gene="hmsP"
                     /locus_tag="JNZ27_002296"
     CDS             10168..12156
                     /gene="hmsP"
                     /locus_tag="JNZ27_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY8664614.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12340..13626
                     /locus_tag="JNZ27_002297"
     CDS             12340..13626
                     /locus_tag="JNZ27_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY8664615.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13847..15343
                     /locus_tag="JNZ27_002298"
     CDS             13847..15343
                     /locus_tag="JNZ27_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8664616.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLIITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15439..16587)
                     /locus_tag="JNZ27_002299"
     CDS             complement(15439..16587)
                     /locus_tag="JNZ27_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8664617.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16600..17367
                     /gene="pdeH"
                     /locus_tag="JNZ27_002300"
     CDS             16600..17367
                     /gene="pdeH"
                     /locus_tag="JNZ27_002300"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY8664618.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKKQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17437..19497
                     /locus_tag="JNZ27_002301"
     CDS             17437..19497
                     /locus_tag="JNZ27_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8664619.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19679..21001)
                     /locus_tag="JNZ27_002302"
     CDS             complement(19679..21001)
                     /locus_tag="JNZ27_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY8664620.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21412..22425)
                     /gene="yhjD"
                     /locus_tag="JNZ27_002303"
     CDS             complement(21412..22425)
                     /gene="yhjD"
                     /locus_tag="JNZ27_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY8664621.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22474..23445)
                     /locus_tag="JNZ27_002304"
     CDS             complement(22474..23445)
                     /locus_tag="JNZ27_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8664622.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            23893..>24492
                     /locus_tag="JNZ27_002305"
     CDS             23893..>24492
                     /locus_tag="JNZ27_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8664623.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5791 a         6166 c         6748 g         5787 t
ORIGIN      
        1 ctcaagcaac aatgaaaatt gtttatcgtt attatttccg ggattaatta ccaaaagcga
       61 acaatggttt aacctgctcc atttattcat tttatccaac catgagcgaa gccgctcggc
      121 aggaatgttt tgccatgcgt tatttgcgca aacaagaata aaaaggtaat tatggggatc
      181 aatagaacac tgcaaatcac gggccaaata gtatagacct ttttcatgat ttagcattga
      241 aaataatttt attttttccg gcccttgaga atcatctaat tgaaagattt tcgccggatc
      301 gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc tgggatgcaa ttgtttgatt
      361 cgccagactg atagcatctt catggcgatc gacgttaaac caccagacgc cgcctgctgg
      421 catatgtcgc agctcatccc ataatgatga gataccgata gagaatacag ggtccacaat
      481 gtccctcatg tcaccatttt gtctgattat cagtttacta gcgaaagcta agaaataaac
      541 ctaccattga aattaaaact tatcagattt agcatgtgaa tcaatttaga aggtcacaag
      601 acgtgtgatt ttagtctttt cgtttttaac tttttctgtt gcgtaacgga aagtcaaaaa
      661 gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg aaatgctcac ggttagaatt
      721 gtatcatgaa tggtacggtt atttcatagg gatcaagcaa aatgaataac aatgaaccag
      781 atactctgcc tgatcccgcg ataggctata tcttccagaa tgatattgtg gcgttaaagc
      841 aggcattttc actgcctgat attgattatg ccgatatttc ccaacgcgaa cagttggccg
      901 cggcattaaa acgctggccg ttgctggcag agtttgcgca acaaaagtag gggattggtg
      961 aatggccgta ctgggattgc agggggtgcg gggaggcgtg gggacaacaa ccatcaccgc
     1021 cgcattagcc tggtcattac aaatgttggg agaaaatgtc ctagtggtcg atgcctgccc
     1081 ggacaacttg ttgcgcctgt catttaatgt tgattttacc caccgtcagg gctgggccag
     1141 agcgatgctg gatggccagg actggcgtga cgctgggttg cgctacacct cgcagctcga
     1201 tttgctgcct tttggtcagt tatccattga agaacaagaa aatccacagc actggcaaac
     1261 ccggctgagc gatatttgct ccggcttaca gcaactaaaa gccagcgggc gttaccagtg
     1321 gattttaatc gacttaccgc gtgatgcctc gcagataacc caccagctgc tgagtttgtg
     1381 cgagcactcg ctggcaatcg tcaatgtgga tgccaactgc catatccgac tgcatcagca
     1441 agcgctgccg gatggcgcac atattttgat taatgacttc cgtattggca gtcaggttca
     1501 ggacgatatt taccagcttt ggttgcaaag ccagcgccga ttactgccga tgctcattca
     1561 tcgtgatgaa gcgatggctg aatgcctggc ggctgaagag atactgacgc tggcgaactg
     1621 gtgcctgttg aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg
     1681 acccggtggt tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat
     1741 cgtcgtcacg gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc
     1801 tggattttta ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac
     1861 ctgtatacgc caacgcctcg cgtccgctgg acccggtccg ttatctcatt caaacatgct
     1921 ggttattgat cggtgcatcg cgcaaagaaa cgccgaaacc gcgcagcggg cattttcagg
     1981 tctgcaaaat attcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag
     2041 ccataaaaca cagcatctgg atgaaaaaaa agagctcggt catttgagtg ccggggcgcg
     2101 gcggttgatc ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac
     2161 tcagccgttt aacccgctga cgcagtttat cttcctgatg ctgctgtggg gggtagcgct
     2221 gatcgtacgg cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac
     2281 cgtttcttgc cgttatatct ggtggcgtta cacctctacg ctgaactggg acgatccggt
     2341 cagcctggtg tgcgggctta ttctgctctt cgctgaaacg tacgcgtgga ttgtgctggt
     2401 gctcggctac ttccaggtag tatggccgct gaatcgtcag ccggtgccat tgccgaagga
     2461 tatgtcgctg tggccgtcag tggatatctt tgtcccgact tacaacgaag atctcaatgt
     2521 ggtgaaaaat accatttacg cctcgctggg tatcgactgg ccgaaagaca aactgaacat
     2581 ctggatcctc gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt
     2641 gaaatatatc gcccgcacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct
     2701 gaaatatgcc aaaggcgagt tcgtgtcgat tttcgactgc gaccacgtac caacgcgatc
     2761 gttcctgcaa atgaccatgg gctggttcct gaaagagaaa cagctggcga tgatgcagac
     2821 tccacaccac ttcttctcgc cggacccgtt tgaacgcaac ctgggacgtt ttcgtaaaac
     2881 gccgaacgaa ggcacgctgt tctatggtct ggtgcaggat ggcaacgata tgtgggacgc
     2941 cactttcttc tgcggttcct gtgcggtgat tcgtcgtaag ccgctggatg aaattggcgg
     3001 cattgctgtc gaaaccgtga ctgaagatgc gcatacttct ctgcgtttgc accgtcgtgg
     3061 ctatacctcc gcgtatatgc gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc
     3121 ggcgcatatc ggtcagcgta ttcgctgggc gcgcgggatg gtacaaatct tccgtctcga
     3181 taacccgctc accggtaaag gactgaagtt tgctcagcgg ctgtgctacg tcaacgccat
     3241 gttccacttc ttgtcgggca ttccacggct gatcttcctg actgcgccgc tggcgttcct
     3301 gctgcttcat gcctacatca tctatgcgcc agcgttgatg atcgccctat tcgtgctgcc
     3361 gcatatgatc catgccagcc tgaccaactc gaagatccag ggcaaatatc gccactcttt
     3421 ctggagtgaa atctacgaaa cggtgctggc gtggtatatc gcaccaccga cgctggtggc
     3481 gctgattaac ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga
     3541 agagtacgtc gactgggtga tctcgcggcc ctacatcttc cttgttctac tcaacctggt
     3601 gggcgttgcg gtaggcatct ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt
     3661 ggtcgtcagt atggtgtggg tgttctacaa cctgattgtt cttagcggcg cagttgcggt
     3721 atcggtagaa agcaaacagg tacgccgatc gcaccgcgtg gagatgacga tgcccgcggc
     3781 aattgcccgc gaagatggtc acctcttctc gtgtaccgtt caggatttct ccgacggtgg
     3841 tttggggatc aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt
     3901 gcttaaacgc ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa
     3961 tgaagttggg ctgaaattaa tgccgctcac cacccagcaa catatcgatt ttgtgcagtg
     4021 tacgtttgcc cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc
     4081 gctggaaagt ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga
     4141 gtttgcgcct tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt
     4201 tgtatcgttt attccgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc
     4261 tttggctcaa caatgatgat aacgcgatga aaagaaaact attctggatt tgtgcagtgg
     4321 ctatggggat gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac
     4381 tgatcaatgc tgagccagct gtagccgccc agacggaaca aaatccgcag gtggggcaag
     4441 tgatgtcagg cgagcaggga gctgatgccc ccatcgtggc gcagaacggt ccttcgcgtg
     4501 atgtgaagct gacctttgcg caaatcgcgc cgccgccggg cagcatggtg ctacgtggta
     4561 ttaacccgaa cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga
     4621 tgctcaacct cgaatacacg ccatcgccat cgttactgcc tgtccagtcg cagttaaagg
     4681 tttatctcaa tgatgagctg atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa
     4741 aaacgctggc gcaaatgccc attaatccac tgtttattac cgacttcaat cgtgtgcggc
     4801 tggagtttgt cggccattat caggacgtgt gcgaaaaccc ggccagcacc acgctttggc
     4861 tggatgttgg gcggagcagt ggactggatc tgacctatca gaccctgaat gtgaagaatg
     4921 acctgtcaca cttcccggtg ccattctttg acccgcgcga taaccgtact aacaccttgc
     4981 cgatggtctt tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc attgtcgcct
     5041 cgtggtttgg ttcgcgttct ggctggcgtg ggcagaacct cccggtgctc tataaccaat
     5101 tgccggatcg caatgccatt gtctttgcaa ccaacgacaa acggccggac ttcctgcgcg
     5161 atcatccggc ggtaaaagcc ccggtgattg agatgattaa ccatccgcag aatccttacg
     5221 tcaaactgct ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta
     5281 tcgctcaggg taacattctg ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc
     5341 tgctaccgcg taagccgtac gatgcgccga actgggtacg taccgatcgt ccggtcacat
     5401 ttggcgaact gaaaacctat gaagaacagt tacaatccag cggccttgag ccagcagcga
     5461 ttaacgtttc gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata
     5521 tggatattaa ttaccgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca
     5581 gcctgaataa ccagttcctg caatccttca acctgagcag caaacaggag gcgaaccgtc
     5641 tgctgctgcg tattccggta ttacaaggtt tgctggatgg caaaacagat gtctctattc
     5701 cggcgctgaa actgggcgcg accaaccagc tgcgcttcga ctttgagtat atgaacccga
     5761 tgccaggcgg ttcggtagat aactgtatta ccttccagcc ggtgcagaat catgtggtga
     5821 ttggtgacga ttccaccatc gacttctcga agtattacca cttcatcccg atgccggatc
     5881 tacgcgcctt tgctaacgcg ggcttcccat tcagccggat ggcggatctg tcgcaaacca
     5941 tcaccgtgat gccgaaagcg cctaacgaag cacagatgga aacgttgctg aatactgttg
     6001 gttttatcgg cgcacagacg ggcttcccgg cgattaatct gacggtgacc gatgatggca
     6061 gcaccattca gggcaaagat gccgacatca tgatcatcgg tggtatcccg gacaaactga
     6121 aagacgataa gcagatcgac ctattggtgc aggcgaccga aagctgggtg aaaacaccga
     6181 tgcgccagac cccgttcccc ggcattgtgc cggacgagag cgatcgcgcg gcagaaaccc
     6241 agtcaacgct gacctcttcc ggtgcgatgg cggcggtgat tggcttccag tcgccgtata
     6301 acgaccagcg cagcgtgatt gcgctgttgg cagatagccc acgcggttat gaaatgctta
     6361 acgatgcggt gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc
     6421 gcgagtacgg tatcaacagc ctacgtgttg gcgacgttta ttacgtaggt catctgccgt
     6481 ggttcgagcg cttgtggtat gcgctggcaa accatccgat tctgctggcg gtgctggcgg
     6541 tgctggcggt gctggcggtg ctggcggtgc tggcggctat cagtgtgata ttgctggcat
     6601 gggtactgtg gcgtctgctg cgaattatta gtcgtcgtcg tcttaacccg gataacgagt
     6661 aattgaagat gaatgtgttg cgtagtggaa tcgtgacgat gctgttgttg gctgccttta
     6721 gtgttcaggc agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc
     6781 aggaagggcg cgtcatcgac cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa
     6841 gttacggtat gttctttgcc ctggcggcta acgaccgtgc agctttcgat aatattctcg
     6901 actggacgca gaacaatctc gctcagggtt ctttaaaaga acgtttgccc gcctggctgt
     6961 ggggcaagaa agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg
     7021 atgtctggat ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata
     7081 ccgacatcgg cagcgcgttg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg
     7141 ggctgggttc catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt
     7201 ttaaccccag ctacatgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt
     7261 ggaccacgct gcgcgaaacc aatcaacatt tattgctgga aaccgccccg aaaggctttt
     7321 cgccagactg ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacat
     7381 tgatcagcag ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg
     7441 atccgcaaaa agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa
     7501 acggttatcc gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg
     7561 tcggtttttc tgccgccatg ctgccttttt tacaaaaccg cgatgcgcag gcagttcagc
     7621 gccagcgcgt ggccgataac tttcccggca gcgataccta ttacaactat gtgctgaccc
     7681 tgtttggaca aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac
     7741 ctgactgggg ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct
     7801 ggccgtcatg ccgatggtcg aggcagcacc aaccatcagc agaccctgcg gatggacagc
     7861 ggcaacacta atgccgtgcg cgggctggcg aatatttacc gccagcagtc gccagaaaaa
     7921 gctgaagcgt ttatcgcctc gctctctgcc agccagcggc gcagcattga tgatatcgaa
     7981 cgcagcctgc aaaacgaccg tctggcacag caggcagagg tactggaaaa ccagggcaaa
     8041 tgggcgcagg cggcagcact tcagcggcaa cgactggcgc tggaccccgg cagcgtatgg
     8101 attacttacc gactttcgca ggatctctgg caggccggac aacgcagcca ggccgatacg
     8161 ttaatgcgca atctggcgca gcagaagccg aacaacccgg agcaggttta cgcttacggg
     8221 ctgtacctct caggtcacaa ccaggacaga gcggcgctgg cgcatatcaa cagcctgccg
     8281 cgcgcgcagt ggaacagcaa tattcaggag ctggttaatc gactgcaaag cgatcaggtg
     8341 ctggaaaccg ctaatcgcct gcgagaaagc ggcaaagagg ctgaagcgga agcgatgctg
     8401 cgtcagcaac caccttccac gcgtattgac ctcacgctgg ctgactgggc gtaacaacga
     8461 cgtgattaca ccgccgcccg cactgcatat cagaatgtcc tgacgcggga gccaactaac
     8521 gccgatgcca ttcttggtct gacggaagtg gatattgctg ccggtgacaa agcggcggca
     8581 cgtagccagc tggcgaaact gcctgccacc gataacacct cgctgaacac tcagcggcgc
     8641 gtggcgctgg cacaggcgca gcttggagat accgcagcgg cgcagcagac gtttaataag
     8701 ttgatcccgc aggcaaaatc tcagccaccg tcgatggaaa gcgcgatggt gctgcgcgac
     8761 ggtgcgaagt ttgaagcgca ggcgggcgat ccaaagcagg cgctggaaac ctacaaagac
     8821 gccatggtcg catccggtgt gactacgacg cgtccgcagg ataacgacac ctttacccga
     8881 ctgacccgta acgacgagaa agatgactgg ctgaaacgcg gcgtgcgcag cgatgcggcg
     8941 gacctctatc gccagcagga tcttaacgtc acccttgagc acgattactg gggttcgagc
     9001 ggcaccggtg gttactccga tctgaaagcg cacactacca tgttgcaggt ggatgcgccg
     9061 tattctgacg ggcggatgtt ctttcgcagt gatttcgtca atatgaacgt cggcagtttc
     9121 tccactaatg ccgatggcaa atgggatgac aactggggca cctgtacatt acaggactgt
     9181 agcggcaacc gcagccagtc ggattccggt gccagcgtgg cggtcggctg gcgaaatgac
     9241 gtttggagct gggatatcgg taccacgccg atgggcttca acgtggtgga tgtggtcggc
     9301 ggcatcagtt acagcgatga tatcgggccg ctgggttaca ccgttaacgc ccaccgtcgg
     9361 cccatctcca gttctttgct ggcctttggt gggcaaaaag actccccgag caataccggg
     9421 aaaaaatggg gtggcgtacg tgccgacggt gtggggctaa gtctgagcta cgataaaggt
     9481 gaagtaaacg gcgtctgggc atcgcttagt ggcgaccagt taaccggtaa aaatgtcgaa
     9541 gataactggc gcgtgcgctg gatgacgggc tattactata aggtcattaa ccagaacaat
     9601 cgccgcgtca caatcggcct gaacaacatg atctggcatt acgacaaaga tctgagtggc
     9661 tactcactcg gtcagggcgg ttactacagt ccgcaggaat acctgtcgtt tgccataccg
     9721 gtgatgtggc gggagcgcac ggaaaactgg tcgtgggagc tgggggcgtc tggctcgtgg
     9781 tcgcattcac gcaccaaaac catgccgcgt tatccgctga tgaatctgat cccgaccgac
     9841 tggcaggaag aagctgcgcg gcaatccaac gatggcggca gcagtcaggg cttcggctac
     9901 acggcgcggg cattacttga acgacgtgtt acttccaact ggtttgttgg cacggcaatt
     9961 gatatccagc aggcgaaaga ttacgcaccc agccatttcc tgctctacgt acgttattcc
    10021 gccgccggat ggcagggtga catggattta ccgccgcagc cgctgatacc ttacgccgac
    10081 tggtaagttt tcagatagcg cctctcttaa tgccgctgcg atcgggtata ctcgggcggc
    10141 aatctgggat ttccgggggg agacaatttg cgcgtaagtc gctcgttaac aatcaagcag
    10201 atggcaatgg tggcagccgt tgtcctggtg ttcgttttta ttttttgcac cgttttgctg
    10261 ttccatctgg tccagcagaa tcgctataac acggctacgc aactggaaag cattgctcgc
    10321 tctgtccgcg aacccttatc ttcagctatt ttgaaaggcg atattcccga agcggaagct
    10381 attcttgcca gcattaaacc ggcaggcgtg gtcagccgtg ccgatgtagt gctgcctaac
    10441 cagttccagg cgctgcgtaa aagttttatt ccagagcgtc cggtgccggt aatggttact
    10501 cgcctgtttg agctaccggt tcaaatctcg ctgggcgtct actcgctcga acgtccggca
    10561 aatccgcagc caatagccta tctggtgcta caggcggatt ccttccgtat gtataagttc
    10621 gtgatgagca ctctctcaac gttagtgacc atttacttac ttttgtcgct tatcctgacc
    10681 gtcgccatca gctggtgcat taaccgcctg attttgcatc cgttacgcaa tattgctcgc
    10741 gaacttaacg ccatcccagc ccaggagctt gttggtcacc aactggcatt accgcgtctg
    10801 catcaggacg atgaaatcgg tatgttggtg cgcagttaca acctcaacca gcaattgctg
    10861 cagcgccatt atgaagaaca gaacgaaaat gcgatgcgct tcccggtgtc ggatttgccg
    10921 aacaaagcct tgctgatgga gatgctggag caggttgtcg cgcgtaaaca aaccaccgcg
    10981 ctgatgatca tcacctgtga aaccctgcgt gatactgcgg gcgtgctgaa agaggcgcaa
    11041 cgagaaattc tgctgctgac gctggtggaa aaactcaaat cggtactgtc gccacgtatg
    11101 atcctcgcgc agattagcgg ttatgacttt gctgtcattg ccaacggtgt acaggaaccg
    11161 tggcacgcaa tcactttggg tcagcaagta ctcactatca tgagcgagcg cctgccgatt
    11221 gaacgtattc aactccgtcc gcactgtagc attggcgtgg cgatgttcta cggcgatctc
    11281 accgccgaac agctttacag tcgcgctatt tctgcggcat ttaccgctcg ccataaaggc
    11341 aagaatcaga ttcagttctt tgatccgcag cagatggaag cagcccagaa gcggttgacg
    11401 gaagagagcg atatccttaa tgcactggaa aatcatcagt ttgcaatttg gttacagcca
    11461 caggtcgaga tgaccagcgg taaactggtc agtgcggaag tgttactgcg tatccagcaa
    11521 ccggatggca gttgggacct gccggatggc ttaatcgatc gcattgagtg ctgtgggctg
    11581 atggttaccg tcggtcactg ggtgctggaa gagtcctgtc gattgcttgc agcctggcaa
    11641 gagcgcggca ttatgctgcc cttgtcggta aacctctctg cgctgcaact gatgcacccg
    11701 aatatggtgg cggatatgct ggaactgtta acccgctatc gcattcagcc gggaacactg
    11761 attctggaag tgacagaaag ccgacgtatt gacgaccctc atgctgcggt ggcaatcctc
    11821 cgtccgctgc gcaatgccgg agttcgggtg gcgctggatg atttcggtat gggctacgca
    11881 gggctgcgtc agctgcagca tatgaaatcg ttgccaatcg acgtactgaa aatcgacaaa
    11941 atgtttgttg aaggcttgcc ggaagatagc agcatgattg ctgcaattat catgctggcg
    12001 cagagcctga acttacaaat gattgccgaa ggcgtggaga ctgaagcaca acgagactgg
    12061 ctggcaaaag cgggcgttgg tattgcccag ggcttccttt ttgctcgccc actccctatt
    12121 gaaatcttcg aagagagtta cctggaagaa aagtagctac cccaaactga ttacaaaact
    12181 ttaaaaagtg ctggtttgtg cgagccagct caaacttttt aacctttttg tttcaattat
    12241 gatccaggta catttctgtg atgttgtctg ggtgttattt taaggccgca ggtaccccca
    12301 taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc tctgtttaaa
    12361 agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg ccatttctat
    12421 cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct cattaagatg
    12481 atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat ggaaagcatg
    12541 aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt cagtaccatc
    12601 gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg aatgaacgtc
    12661 gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc gaaagaccag
    12721 ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc atttgccagc
    12781 ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct ccaccgtctg
    12841 ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt tctcgcaggt catcttcggc
    12901 atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat ggcgtttacc
    12961 atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat ctgtttctac
    13021 attacctgta tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc gactggtttc
    13081 agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact ggggacttca
    13141 tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg ctgccgtaaa
    13201 tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg cacatcgata
    13261 tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat ggatatcgtc
    13321 caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc aggggtaacg
    13381 ggtagtggct ttatcgtgct ggcggcgacg ctttctgcgg tgggccattt gccggtagcg
    13441 ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc gctaactaac
    13501 ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga actggaccac
    13561 aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca cgaattatcc
    13621 tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt tgcgcataat
    13681 tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac ttcttccgtg
    13741 acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta gactgaccga
    13801 caacgggcag tttttttatt accaggatag ttgatcaggg gttcacatgc agggcataaa
    13861 aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg tgcaggcaga
    13921 tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt tacagtggca
    13981 agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc tggttaatac
    14041 cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc ctcgtattgc
    14101 gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc agcaggggat
    14161 cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc tgtttaatct
    14221 gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc tggcaaatgc
    14281 cactggcaaa ttgatcatca caccagaaac catcaaccac gcgctgcaaa gtcaggacat
    14341 ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc tgaaagggtc
    14401 aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag cggaaaaaat
    14461 taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg tggtgggaaa
    14521 cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac tgaaaggcaa
    14581 acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag cggtgagtat
    14641 tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc cgtggcagcc
    14701 gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc gcgaggcgct
    14761 gttctggcat gttcagcaag cgttaagcgc cagtgacagc aaagacatcg gtcttggatt
    14821 tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat caccaaacga
    14881 caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc gcgataaagg
    14941 tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc tgcagaaact
    15001 gtttgccgcc tatgcacggg ctgataccga tattctgatg ggccagcgga tgcgttcgtt
    15061 gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc aggatttcct
    15121 taacagcctg actgtggaga tgttaaatca ggatctgcgt cagcagttgt cgaatgatat
    15181 ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag cgttgcaggc
    15241 ggcctgggat caaatcatgg ccccatctac cgccgctgcg accacctctg tcgccacgga
    15301 tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga tggtgatgag
    15361 ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac tttatccagc
    15421 cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg cgccgcgata
    15481 ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg cactgccgcc
    15541 cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg tggtatcgat
    15601 cactttttct ttcggcagtt tcaccgccgg aacatcaact aacgcttcac cagcaatgga
    15661 caccaggcaa gaacccgccc cgcgtttcac caccacttct ttcacgcccg cgttatgggt
    15721 gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcgt cgtccagcgt
    15781 caggaaggcg atatccgtgc attccagcat ttgctggtac acctgctgtg tctcttcttt
    15841 gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc ctttggcgcg
    15901 gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga tcgccaggct
    15961 aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg ccgcagactg
    16021 ctcgctcgcc agccagaatt tggcggcggc ttcgttacgc cagtagtaga acgtacgttc
    16081 gccggtgctg tcggtttcaa tgtagtaaag gcccggaaga cggttttcca tccgttgggt
    16141 cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atctgctggc taaaactgtc
    16201 cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct gacgggcgat
    16261 atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg cgtctttctc
    16321 ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag tcgtgaacct
    16381 gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa ccatattaaa
    16441 acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg ttgtggagtg
    16501 aggaaattta ccccggccag gcggtaaagt tctgccctta cgcgccgata atctttgtcg
    16561 agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca ggttatccag
    16621 cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt ttggttgcag
    16681 tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcgggcggtt aatggccgtg
    16741 gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc gccggatcgc
    16801 tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaaaagca gattgatttg
    16861 ctggcgcaaa aagccgactt ctttgtagag cacggcctgc tggcatcggt caatattgat
    16921 ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat tgagcgtctt
    16981 ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc aacctttgcc
    17041 tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat ggcaaatttc
    17101 tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact gtttgtgatg
    17161 ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct aatgaatcgc
    17221 tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg tgatgttcag
    17281 aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc gatggaaacg
    17341 ctgaatacgg cggttctggc gctataagct gcctcatttt ccgccgggct ggactatctt
    17401 taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg caaaataacc
    17461 gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt gattgcaact
    17521 tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga gttgaatcgt
    17581 ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca agaaactggc
    17641 tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct tggtaatcca
    17701 ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac gctggcaccg
    17761 ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa acccgacgcg
    17821 cgcctgattc gcctctctga aaagaacaat aactggacgt ttaatcttgc caacgatgac
    17881 aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa tattcttttc
    17941 gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga aatttttgtt
    18001 gatccgttag gcaagccgct gccattcagc gaagttactg gatcgaaagg taaagcggat
    18061 aaagaaaagg tgggcgatta catttttggc ctgaaggcgc agggacgtta taacggtgaa
    18121 ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga agggacgccg
    18181 tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga tggcgtcgtg
    18241 aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg cgattcactg
    18301 ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt tgaaacggat
    18361 ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta tcgcggtttt
    18421 aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac cggaaagcca
    18481 cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga cctgggaccg
    18541 ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga acagaagaag
    18601 ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt cgaaaccgat
    18661 aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga gcatggcagt
    18721 agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga cctgcgcctg
    18781 caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca tctggaaggc
    18841 gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa actgaaagaa
    18901 ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg tgacgcggaa
    18961 ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg caacctgaaa
    19021 ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg gctgaatgtc
    19081 ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa ctgcgcggcg
    19141 gcgaatctgc atattgccaa cggcgtggcg cgcccgcaga tttttgcttt cgatactgag
    19201 aacgcgttga ttaatgttac cggcacggca agttttgctt cggaacagct ggatttgact
    19261 attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct gtatgtgcgt
    19321 gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc ccgtggtgct
    19381 gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc actgatctca
    19441 ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa gaagtgatga
    19501 taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg caaagtgtgc
    19561 tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct tgtgaattca
    19621 atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt cacacttatt
    19681 acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc aagccagcca
    19741 tcgccgccag atataacccc accgcaccta acccgtagtt agcctgcaac caagctgcga
    19801 tatacggcgc aacggaagcc ccgagaatcg acgatacgtt gtaagagaac gatgctccgg
    19861 tgtaacgcac ttctgtcgga aacagctctg gtaacagcgc acccatcggc ccgaaggtca
    19921 gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg ttgccagaac
    19981 cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg atgatcacca
    20041 tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc atcacgccaa
    20101 aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg ccaagcccaa
    20161 ctggtgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa aacagcgtat
    20221 aggttgccag cataatgaac gtacccagta cggttacgcg aacatgtttg gtcagcagcg
    20281 tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac accggcgact
    20341 catgcagcga cacgcgaaca tacaggccga taatgaccag caccgccgag aagataaatg
    20401 gcacacgcca gccccagctc ataaactgct catcggtcag tagccaggaa agcagcaaga
    20461 aagtgccgtt ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag gagccataca
    20521 gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg ccccattcac
    20581 cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagtggg gcgaaaatac
    20641 caatcgtggc atagcccggc agcagaccaa tcaccacggt cgaaatcccc atcgttagca
    20701 acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca aaaacggcag
    20761 agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt agcgttgctg
    20821 ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc gcagtggcgt
    20881 aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca aggactttat
    20941 tacgcgagtt gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct gttgcttgca
    21001 taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa aagtgatatt
    21061 tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga taatgtttag
    21121 gccagtgatt tttcttctat aaaatgcttc acataatttt ataaatagtg gataaaactg
    21181 gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg agttggagtg
    21241 aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt caagaggtta
    21301 tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg gggagccagg
    21361 ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat tttaaggctg
    21421 cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca
    21481 aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca cggaaccaaa
    21541 tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca cgattttaat
    21601 cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt tgcgcggacg
    21661 gtggcgcggc aaccgccaga agatccagaa gaaaagcaga tagttggcga agatggaaat
    21721 cgccaggcca atcaatcgcc acgtcggttt cagccactca atgctgttca ggtgcagggc
    21781 gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg tcacaatcag
    21841 cgcaatcaac aaaccaatca gtgaaataaa atcacgcaga tattttaccc agaacttctc
    21901 ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcacgaatcg cttcacgcag
    21961 gttacccatc cagttgatgc cggaataaag tgccaccgcc aggccgacaa gccctacagt
    22021 cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta gtgtcggatc
    22081 gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat gggaggccag
    22141 cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca aaaatgagaa
    22201 ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg
    22261 aataaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg tgacggtttc
    22321 cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa tcggctcgtg
    22381 ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct cttttgtcct
    22441 tctgtcgtgg tcattcttaa aaagtatagt cggtcagtcc aggtgctctt tcattacgcc
    22501 agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct gcgggtaaac
    22561 cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa gccgcccggc
    22621 acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac cggcaagacc
    22681 ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact taaaccactg
    22741 gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt gcggtgtata
    22801 acgcactatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa agcgcgtcag
    22861 atagtgcgga ctggcacagt tgaccatggt tagtttgccg aggggacggg cgataacgcc
    22921 atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa gaatatctac
    22981 cggacggtta tggctactca gttccagctc aattcccgga tgctgataga gaaattccga
    23041 cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc gtaatttacc
    23101 gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat tactcaacac
    23161 atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc gtcgcgtagt
    23221 gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc gcgaaacact
    23281 tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct ccgcgacttt
    23341 gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt gttcacagcg
    23401 aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat gccgggtttc
    23461 acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga atagctttat
    23521 gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt aaagtgaata
    23581 gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta ttttggcttg
    23641 tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa ggaaatcctc
    23701 aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg taactattta
    23761 tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa atggcgaatt
    23821 cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac gtattcaggt
    23881 ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat tcatggaatg
    23941 aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggtcag tcaggcagac
    24001 gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga tggcgatcag
    24061 gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc tgaggttaag
    24121 gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat ccattttaat
    24181 gtgctggcca ttgtgcctcg cgattcaacc gtcgagacat ttgcgttggc ggtaaatagt
    24241 gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga aaaggacatt
    24301 aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt agcagcgggc
    24361 gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt aaaagcgcat
    24421 ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc aatgatgtta
    24481 aatatctcct cc
//
LOCUS       DADMFL010000058        24191 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.058, whole genome shotgun sequence.
ACCESSION   DADMFL010000058 DADMFL010000000
VERSION     DADMFL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.058"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(25..597)
                     /locus_tag="JNZ27_002306"
     CDS             complement(25..597)
                     /locus_tag="JNZ27_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664624.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="JNZ27_002307"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="JNZ27_002307"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY8664625.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="JNZ27_002308"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="JNZ27_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY8664626.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="JNZ27_002309"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="JNZ27_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY8664627.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="JNZ27_002310"
     CDS             complement(3559..5340)
                     /locus_tag="JNZ27_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8664628.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSCNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="JNZ27_002311"
     CDS             complement(5333..7105)
                     /locus_tag="JNZ27_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8664629.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /locus_tag="JNZ27_002312"
                     /pseudo
     CDS             complement(7135..7593)
                     /locus_tag="JNZ27_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="JNZ27_002313"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="JNZ27_002313"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY8664630.1"
                     /translation="MARLAAFDMDGTLLMADHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10364
                     /locus_tag="JNZ27_002314"
                     /pseudo
     CDS             8665..10364
                     /locus_tag="JNZ27_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10429..11124
                     /gene="queC"
                     /locus_tag="JNZ27_002315"
     CDS             10429..11124
                     /gene="queC"
                     /locus_tag="JNZ27_002315"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY8664631.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11176..11574)
                     /locus_tag="JNZ27_002316"
     CDS             complement(11176..11574)
                     /locus_tag="JNZ27_002316"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY8664632.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11668..12039)
                     /locus_tag="JNZ27_002317"
     CDS             complement(11668..12039)
                     /locus_tag="JNZ27_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664633.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12190..14061)
                     /gene="ppiD"
                     /locus_tag="JNZ27_002318"
     CDS             complement(12190..14061)
                     /gene="ppiD"
                     /locus_tag="JNZ27_002318"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8664634.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14253..14525)
                     /gene="hupB"
                     /locus_tag="JNZ27_002319"
     CDS             complement(14253..14525)
                     /gene="hupB"
                     /locus_tag="JNZ27_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY8664635.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14734..17088)
                     /gene="lon"
                     /locus_tag="JNZ27_002320"
     CDS             complement(14734..17088)
                     /gene="lon"
                     /locus_tag="JNZ27_002320"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY8664636.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQDVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIAYLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17276..18550)
                     /gene="clpX"
                     /locus_tag="JNZ27_002321"
     CDS             complement(17276..18550)
                     /gene="clpX"
                     /locus_tag="JNZ27_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY8664637.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18676..19299)
                     /gene="clpP"
                     /locus_tag="JNZ27_002322"
     CDS             complement(18676..19299)
                     /gene="clpP"
                     /locus_tag="JNZ27_002322"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY8664638.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19546..20844)
                     /gene="tig"
                     /locus_tag="JNZ27_002323"
     CDS             complement(19546..20844)
                     /gene="tig"
                     /locus_tag="JNZ27_002323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY8664639.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21188..21505)
                     /gene="bolA"
                     /locus_tag="JNZ27_002324"
     CDS             complement(21188..21505)
                     /gene="bolA"
                     /locus_tag="JNZ27_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY8664640.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21810..22388
                     /locus_tag="JNZ27_002325"
     CDS             21810..22388
                     /locus_tag="JNZ27_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8664641.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22432..23907
                     /gene="ampG"
                     /locus_tag="JNZ27_002326"
     CDS             22432..23907
                     /gene="ampG"
                     /locus_tag="JNZ27_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY8664642.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5671 a         6662 c         5835 g         6023 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggagc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta ttgcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 agatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccgctga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcccacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tcagccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacgt tggcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg caagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cacgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgttgtaa cgctggtata tcaccattac
     4801 cacaagcacc actgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggagatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actacgccta ctgccgcccg attaaacagc agcgactgag cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccag ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgagttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggat cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctagacg cgcatcagat aatcgtattc accagccatg cgccagaacc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtcag
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagc
     8521 catcaataaa gtgccatcca tatcaaatgc tgccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgg aaagccgcaa accgtcaccg tcagcgaact ggccgaacgc
     8761 tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc aggcacagga ggcgggatgg
     8821 ctggagtggc aggcgcagtc aggacgcgga aagcgcggac aattacgctt tctggtcacg
     8881 ccggaatcgc tacgcaatgc gatgatggaa caggcactgg aaaccggaaa gcagcaagat
     8941 gtgctggagc tggcgcaact ggccccaggt gagctgcgca ctctgttaca gccgtttatg
     9001 ggcggacaat ggcaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac
     9061 cgctacaacc aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt
     9121 ccggcctgac ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact
     9181 gggaaacctc tactgacggg ttacgctggg acttttatct tcgttcaacc ctacactggc
     9241 ataacggcga tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac
     9301 tgccagcact ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt
     9361 gtctgacctt ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt
     9421 gcagccatct ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac
     9481 aattcacagc ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc
     9541 tgcttaaagc ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca
     9601 gttgtcggca tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc
     9661 aggtcagtag tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac
     9721 tctccctctg gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa
     9781 cgttagaagt cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta
     9841 ttccgcactg gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc
     9901 acctaccgat agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag
     9961 gctgtgaact cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg
    10021 cacacgcaga cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg
    10081 agcaatggct acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac
    10141 atctgcaatc gacgctggac gcggcgcaag taatgcctga tgaggaaaac cgatttaatg
    10201 ccctgaaagc ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact
    10261 atcactatcg cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct
    10321 ggtttgaatt taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt
    10381 aatcgcaggc gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct
    10441 gtcgttgtgt tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa
    10501 tatgatgaag tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac
    10561 gtggcacgcg aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg
    10621 ctgctcaacg agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat
    10681 gaacctgaag ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg
    10741 acgctggcgg caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt
    10801 gaaacggatt tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat
    10861 gccgtcagtt tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat
    10921 aaagcggaaa cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa
    10981 acgttgacct gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat
    11041 ttacgcgcca acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg
    11101 aagcagaaaa ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc
    11161 ggcaccacaa aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag
    11221 agctaatgct ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac
    11281 cacctgccct tccggctcca gtgtaatgac ctggcttaag atgccgcttt taccgtttaa
    11341 ttgctgcaac tgactggtaa tagttaacag gtcgcttaat accgctgggc gacgatagtt
    11401 aatattgata ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc
    11461 gctattttcc aacccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt
    11521 gacgtgctgg taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca
    11581 ataaccttac gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg
    11641 gcaaaaaatt gccactatgc aaattaatta cagggttaat accgccagat tacgttccac
    11701 cagcgaattg cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta
    11761 ctcttcacga taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg
    11821 ggctagctct tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc
    11881 tttcgccggt actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc
    11941 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
    12001 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
    12061 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
    12121 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
    12181 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
    12241 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
    12301 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
    12361 cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
    12421 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
    12481 gccaaatttc agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac
    12541 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
    12601 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac
    12661 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc
    12721 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
    12781 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
    12841 ttgctctgca ccggccagag actcggtttc gttgcttgcc gcatcgctca ctttctgctg
    12901 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
    12961 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
    13021 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
    13081 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
    13141 gataatatcg gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
    13201 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
    13261 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
    13321 aaccggttgc tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg
    13381 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
    13441 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
    13501 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc
    13561 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
    13621 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
    13681 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
    13741 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
    13801 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
    13861 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
    13921 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
    13981 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
    14041 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
    14101 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
    14161 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
    14221 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
    14281 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
    14341 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
    14401 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
    14461 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
    14521 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
    14581 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
    14641 atccgctatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
    14701 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
    14761 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
    14821 gtccagatag gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
    14881 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
    14941 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
    15001 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
    15061 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
    15121 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
    15181 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
    15241 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
    15301 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
    15361 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
    15421 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
    15481 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
    15541 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
    15601 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
    15661 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
    15721 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
    15781 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
    15841 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
    15901 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
    15961 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
    16021 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
    16081 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
    16141 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
    16201 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
    16261 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
    16321 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
    16381 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
    16441 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
    16501 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
    16561 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
    16621 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
    16681 gctgattgca gtacgcacca gcacgtcctg ttcccgctca tcaatggtcg gcgactccag
    16741 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
    16801 ctgtaacccc tcgaccagca ctttgactgt gccgtcaggc agtttcagca tctgcaatat
    16861 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
    16921 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
    16981 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
    17041 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
    17101 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
    17161 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
    17221 aaaggggaga taaaatcccc cctttttgtt taactaattg tatgggaatg gttaattatt
    17281 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
    17341 caattaccga ctcgtcaata accacttttt cgacgtcttc catggacggc agatcgtaca
    17401 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
    17461 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
    17521 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
    17581 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
    17641 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
    17701 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
    17761 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
    17821 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
    17881 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac
    17941 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
    18001 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
    18061 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
    18121 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
    18181 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
    18241 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
    18301 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
    18361 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
    18421 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
    18481 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
    18541 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
    18601 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca
    18661 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
    18721 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
    18781 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
    18841 agcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
    18901 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc
    18961 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
    19021 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
    19081 gatatctttt tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc
    19141 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
    19201 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
    19261 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
    19321 gaaaaaaacc tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt
    19381 tatgggtacg gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg
    19441 aaagaggctt ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg
    19501 cggtgacggg cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt
    19561 catcagctcg ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc
    19621 aacagcctgt tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct
    19681 gtagaactcg ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc
    19741 tttcacgcgc tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag
    19801 gccaacaact acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc
    19861 ttgtttttcg ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct
    19921 gtcgatcagc gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg
    19981 agacttaacg cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc
    20041 agcgcgcaga ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt
    20101 cagttccggc agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt
    20161 acctttcagg ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc
    20221 gccagctttg tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat
    20281 cgccagtacg aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt
    20341 gaagtcgatg gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc
    20401 ctgctgttta cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaactat
    20461 cggtttttca acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa
    20521 ctctacagag taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc
    20581 gccagccgga ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc
    20641 acccagaacg tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac
    20701 tttgcctttg cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt
    20761 aacagcggtc tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg
    20821 agtggtttca actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact
    20881 cttaccccgc aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca
    20941 cattccctgt taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc
    21001 atcactttta tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc
    21061 gggcaatttt cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc
    21121 cgcaggccgt ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa
    21181 tgcgttttta cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct
    21241 gcaacccttc ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag
    21301 agagttcctc cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg
    21361 taaaacgatc gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt
    21421 gacgatagct ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt
    21481 cttctatccg ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc
    21541 atccctttaa atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag
    21601 gtttcgtcct ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca
    21661 ggcctcgcat cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac
    21721 atgccttacc cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa
    21781 cacgcttaag agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg
    21841 ctctgtttat gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga
    21901 ttacactgcc acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg
    21961 atcagcgtac agatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga
    22021 ccgcctcccg cgatctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc
    22081 gtggttacat ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt
    22141 atgctgacgt gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca
    22201 tcatcgctac cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg
    22261 ttgaaggtgc gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga
    22321 ccgataccat cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg
    22381 cgcgttaatt tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt
    22441 caatatttac gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct
    22501 tccgggctac cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat
    22561 atcgatctca aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc
    22621 ctctggtcac cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg
    22681 ctgctcgcca cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca
    22741 ggcacccaac tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc
    22801 caggatattg tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg
    22861 ggcgcggcaa tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc
    22921 ctgtggctgg cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg
    22981 ttgatcccct gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg
    23041 cccaaaacgc tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat
    23101 gcctggctta ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc
    23161 ctgacaacca cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta
    23221 aacaaaacgc ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg
    23281 cagcgcctgt cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac
    23341 gctggttact ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc
    23401 tttttcgaaa acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg
    23461 ctgtgtaata agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta
    23521 gggcgagttt atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca
    23581 ttctatctat tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc
    23641 cagacgcttg aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca
    23701 ggttatgcct ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta
    23761 cttctgttga cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg
    23821 gaagtcgggg ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg
    23881 gcgctacgaa aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc
    23941 gccgtaatta ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg
    24001 cggcttagcg tttggaaatt attggcgcca tttataaatt ctatttttct tacacgattc
    24061 agctaatgag tctttatttt ctcaccaccc agttgtcact ctaatgataa ttatttgtta
    24121 aataattgtt ttatttcgca ttggttatac caattgcccg ccaagactcc ttctgcactc
    24181 cccttttgtt a
//
LOCUS       DADMFL010000059        23694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.059, whole genome shotgun sequence.
ACCESSION   DADMFL010000059 DADMFL010000000
VERSION     DADMFL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.059"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JNZ27_002327"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JNZ27_002327"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY8664643.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JNZ27_002328"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JNZ27_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY8664644.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JNZ27_002329"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JNZ27_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY8664645.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JNZ27_002330"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JNZ27_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY8664646.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3880..3924
                     /locus_tag="JNZ27_002331"
     CDS             3880..3924
                     /locus_tag="JNZ27_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY8664647.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JNZ27_002332"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JNZ27_002332"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY8664648.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JNZ27_002333"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JNZ27_002333"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY8664649.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JNZ27_002334"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JNZ27_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY8664650.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JNZ27_002335"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JNZ27_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY8664651.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JNZ27_002336"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JNZ27_002336"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY8664652.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JNZ27_002337"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JNZ27_002337"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY8664653.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JNZ27_002338"
     CDS             10418..10882
                     /locus_tag="JNZ27_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8664654.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JNZ27_002339"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JNZ27_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13331
                     /locus_tag="JNZ27_002340"
     CDS             11895..13331
                     /locus_tag="JNZ27_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY8664655.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNZ27_002341"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNZ27_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY8664656.1"
                     /translation="MRYTDSRKLMPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNZ27_002342"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNZ27_002342"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY8664657.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNZ27_002343"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNZ27_002343"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY8664658.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JNZ27_002344"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JNZ27_002344"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY8664659.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNZ27_002345"
                     /pseudo
     CDS             complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNZ27_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JNZ27_002346"
     CDS             complement(20164..20457)
                     /locus_tag="JNZ27_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8664660.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JNZ27_002347"
     CDS             complement(20454..21743)
                     /locus_tag="JNZ27_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8664661.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="JNZ27_002348"
     CDS             complement(21799..22737)
                     /locus_tag="JNZ27_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY8664662.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSMDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="JNZ27_002349"
     CDS             complement(22757..23521)
                     /locus_tag="JNZ27_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY8664663.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5841 a         5779 c         6252 g         5822 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcggacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcatg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggggccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggata
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagggg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc catgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aatagtgtgc gatagcggtg cgggggggtc gacggagcaa gctgttttcc ttgccaacaa
    23641 tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat ttgcggcaat atag
//
LOCUS       DADMFL010000060        22599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.060, whole genome shotgun sequence.
ACCESSION   DADMFL010000060 DADMFL010000000
VERSION     DADMFL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.060"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..610
                     /locus_tag="JNZ27_002350"
     CDS             <1..610
                     /locus_tag="JNZ27_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8664664.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFR
                     PAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            631..1291
                     /gene="csiR"
                     /locus_tag="JNZ27_002351"
                     /pseudo
     CDS             631..1291
                     /gene="csiR"
                     /locus_tag="JNZ27_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1292..1741)
                     /gene="kbp"
                     /locus_tag="JNZ27_002352"
     CDS             complement(1292..1741)
                     /gene="kbp"
                     /locus_tag="JNZ27_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY8664665.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1825..1983)
                     /locus_tag="JNZ27_002353"
     CDS             complement(1825..1983)
                     /locus_tag="JNZ27_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY8664666.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2166..2465
                     /locus_tag="JNZ27_002354"
     CDS             2166..2465
                     /locus_tag="JNZ27_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8664667.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2475..2999
                     /gene="ygaP"
                     /locus_tag="JNZ27_002355"
     CDS             2475..2999
                     /gene="ygaP"
                     /locus_tag="JNZ27_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY8664668.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3046..3450)
                     /gene="stpA"
                     /locus_tag="JNZ27_002356"
     CDS             complement(3046..3450)
                     /gene="stpA"
                     /locus_tag="JNZ27_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY8664669.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMFEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4118..4555
                     /gene="alaE"
                     /locus_tag="JNZ27_002357"
     CDS             4118..4555
                     /gene="alaE"
                     /locus_tag="JNZ27_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY8664670.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4592..4942)
                     /locus_tag="JNZ27_002358"
     CDS             complement(4592..4942)
                     /locus_tag="JNZ27_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY8664671.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            5094..5423
                     /locus_tag="JNZ27_002359"
     CDS             5094..5423
                     /locus_tag="JNZ27_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8664672.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            5671..5916
                     /gene="nrdH"
                     /locus_tag="JNZ27_002360"
     CDS             5671..5916
                     /gene="nrdH"
                     /locus_tag="JNZ27_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY8664673.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5913..6323
                     /gene="nrdI"
                     /locus_tag="JNZ27_002361"
     CDS             5913..6323
                     /gene="nrdI"
                     /locus_tag="JNZ27_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY8664674.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6296..8440
                     /gene="nrdE"
                     /locus_tag="JNZ27_002362"
     CDS             6296..8440
                     /gene="nrdE"
                     /locus_tag="JNZ27_002362"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY8664675.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8450..9409
                     /gene="nrdF"
                     /locus_tag="JNZ27_002363"
     CDS             8450..9409
                     /gene="nrdF"
                     /locus_tag="JNZ27_002363"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY8664676.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9764..10966
                     /locus_tag="JNZ27_002364"
     CDS             9764..10966
                     /locus_tag="JNZ27_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY8664677.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10959..12023
                     /gene="proW"
                     /locus_tag="JNZ27_002365"
     CDS             10959..12023
                     /gene="proW"
                     /locus_tag="JNZ27_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY8664678.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12081..13073
                     /gene="proX"
                     /locus_tag="JNZ27_002366"
     CDS             12081..13073
                     /gene="proX"
                     /locus_tag="JNZ27_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY8664679.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13265..14444
                     /locus_tag="JNZ27_002367"
                     /pseudo
     CDS             13265..14444
                     /locus_tag="JNZ27_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14568..15305
                     /locus_tag="JNZ27_002368"
     CDS             14568..15305
                     /locus_tag="JNZ27_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY8664680.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15295..15630
                     /gene="ygaH"
                     /locus_tag="JNZ27_002369"
     CDS             15295..15630
                     /gene="ygaH"
                     /locus_tag="JNZ27_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY8664681.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15721..16251
                     /gene="emrR"
                     /locus_tag="JNZ27_002370"
     CDS             15721..16251
                     /gene="emrR"
                     /locus_tag="JNZ27_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY8664682.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16378..17550
                     /gene="emrA"
                     /locus_tag="JNZ27_002371"
                     /pseudo
     CDS             16378..17550
                     /gene="emrA"
                     /locus_tag="JNZ27_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17567..19105
                     /gene="emrB"
                     /locus_tag="JNZ27_002372"
     CDS             17567..19105
                     /gene="emrB"
                     /locus_tag="JNZ27_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY8664683.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19169..19684)
                     /gene="luxS"
                     /locus_tag="JNZ27_002373"
     CDS             complement(19169..19684)
                     /gene="luxS"
                     /locus_tag="JNZ27_002373"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY8664684.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19834..21390)
                     /gene="gshA"
                     /locus_tag="JNZ27_002374"
     CDS             complement(19834..21390)
                     /gene="gshA"
                     /locus_tag="JNZ27_002374"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8664685.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21463..21891)
                     /locus_tag="JNZ27_002375"
     CDS             complement(21463..21891)
                     /locus_tag="JNZ27_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8664686.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(21888..22454)
                     /gene="yqaB"
                     /locus_tag="JNZ27_002376"
     CDS             complement(21888..22454)
                     /gene="yqaB"
                     /locus_tag="JNZ27_002376"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY8664687.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5436 a         5762 c         5666 g         5735 t
ORIGIN      
        1 gccggggctg aaagccgttg gatcttatcg ctcggttctg gaattgctca atattcccca
       61 tgcgaaatta atcatggact gtgtgatatt actttccgta accagttgcc tgaactcggc
      121 cctgtatacc gcctcaagga tgctctactc cttaagccgt cgcggtgatg ctcccgcggt
      181 aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc
      241 agcattctta acggtggtgg tgaactatta cgcgcctgcg aaggtattta aatttctgat
      301 cgacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacagttgcg
      361 gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ttgcgaatgt ggctttatcc
      421 ctggctcacc tggctggtca tcggctttat tacctttgtg ttggtagtga tgctattccg
      481 tccggcgcaa cagttagaag tgatctccac cggcttatta gcgataggga ttatctgtac
      541 cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa
      601 tacgcgctga taatgatcag gagtcacacc atggccatta cgtctctgga tggctatcgc
      661 tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt
      721 ttactgacat cgcgttatgc acttggcgtt gggccgttgc gggaagcact ttctcaactg
      781 gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca
      841 gagcaggagc tgctctgaca tagacgcccg cgccaatatg gaagcgatgc tggtcagtct
      901 ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag cgcatctgct
      961 caataagctg gaagcctgtg acgccagcga gaaaatgctt gatgagtggg atctgcgtca
     1021 tcaggcgttt catacagcaa ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg
     1081 tgaacggttg tttgatctgg cggcgcgtta tcgatttatc tggctgcggc gaacggtgct
     1141 ttcggtggaa atgctggagg ataaacacga tcagcaccag accctgactg cggcggtact
     1201 ggcgcgagat acagcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc
     1261 cattatccag caggcgatgg ctggcaatta actactcttc cggaatacgc aacacttgcc
     1321 ccggataaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
     1381 acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
     1441 cggtataaaa ctggctggca gtggctggtg tcgccgtttt cacctgatca tcaacactgg
     1501 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgactcaggc
     1561 cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcggcatcag
     1621 gtataccggt tttgctcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
     1681 gacctgtaac cgcatcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
     1741 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
     1801 aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa
     1861 accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
     1921 tttaccgagc agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc
     1981 catatgtact ccctataaga aaattactca ttgtttaaaa agagacttta tctcttaagc
     2041 gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca tttacgctgc
     2101 tgaggctggt atatgttgca tttttagttt agagtttact taatttagaa aatacttaaa
     2161 taaatatgac tgaactcgcg caattacagg ccagtaccga acaggcagcg gccttattga
     2221 aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt tcccccggca
     2281 ccagcgcggg agagctgacg cgcattaccg gactgagcgc ctctgcgaca tcacagcatc
     2341 tcgctcgtat gcgggacgaa gggcttatca acagccaacg ggatgcccaa cgcattctat
     2401 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
     2461 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
     2521 cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt gaacatattc
     2581 ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttcca cctaaattac
     2641 gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata
     2701 aattagcagc gattgccgcc cccgcagaaa tatttttact cgaagatggg attgatggct
     2761 ggaaaagagc tggattgcca gtagcggtaa ataaatctca acccttgccg ttaatgcgcc
     2821 aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa
     2881 atagcggttt cttcttatta agtggctttg ttggtgccgg gttactgttt gcaggaatca
     2941 gcggtttttg tggaatggca aggttgttag ataagatacc gtggaaccaa cgagcttgag
     3001 aagcgacgcc ggacgcgccc tagcagcgac atccggcctc agtaattaga tcaggaaatc
     3061 gtcgagagat ttaccttctg ccagcgcctg agcaattggc ttcggtgtac ggccctgacc
     3121 ggtccaggtt ttagtttcac cgttaacatc agtgaattta tatttcgccg gacgcggctg
     3181 gcgtttttta ccagcacgtg gtgcagcagc agagctatta cccaataact cttccgggtt
     3241 aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctgac gctcagccag
     3301 ttcacgctgc tgctgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
     3361 gaacatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
     3421 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa
     3481 aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa aatttaatct
     3541 atttacagtc ctgcgcgaga ttatttcagc tgttattttc tgaaactctc agaataattt
     3601 caagattata tattagccat tttgatgagt aatttaggtt attggtgaac agagtggcgc
     3661 tctctgctcg ctaactccgt aagcctgaca aaaccgaagt gaataaaaat gcaatataac
     3721 agattaaaag aaaacacccc tcactggtgc tttctgctgt taatgtacat tttctggcat
     3781 caaaaatgct gtaaatgacc cggtcacgac aaacaccgaa ttctctacgc ttatagcgtt
     3841 acctcccccc caaactttca ttagtgaaat taaaggcaag gccatatcca attgatttaa
     3901 ttgaaaataa atcatatcgg catgtaaaaa tccacaatgt acaaaaaacg agccgttacg
     3961 gaatgtttta tctacaaaaa ctgactaaat aaaaattttt cactaattga ttagtcatag
     4021 ccagcgatat acgctatgcg aaaatacaga tggcaatgag atccactgct ttcatctcca
     4081 ttaacatccc attacgcttt tattaaggag cattagcatg ttctcaccgc agtcacgctt
     4141 gcgtcatgca gttgcagata cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg
     4201 tattgaagtt ttcctctccg gaatgagctt cgaacagtct ttttattcca gattggtagc
     4261 gattccggtg aacatcttaa ttgcatggcc atacggtatg taccgcgatc tgtttatgcg
     4321 cgcggcacgc aaaggctgga taaaaaatct ggctgatatc ctggcttatg tgacgttcca
     4381 gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc agattatggc
     4441 ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg gctacttcct
     4501 cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag cctgactctt
     4561 cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat aaaccattca
     4621 aataacgttc aagcgcaaca cgcgaactaa agccatgcgg gataccgtaa tgctcgtgtc
     4681 gctgtcgctc gcctgctaaa tagagcggaa attgctggta atgatcgcaa gttttaatat
     4741 cggaagcggg ttcagccagt gttgaactgc gttcttttaa tgttggatga attaccagag
     4801 cggtacgtcc cattcgcgct tcgcgattaa catagacata attttcccca cggcggtaac
     4861 catacgcttt ctgggttacc acatcgacag taaaaccgac tttttcaagt acgcgcgcca
     4921 cctcgtctgg tcgtaaatac atatttgatc ctcgttattt tcaaccgcgg gcctacctta
     4981 cctgattgcg cattcacaac gctttcagaa aagtccataa agccgcgatg aacctcagtt
     5041 aagaaaatta tggtctacac tgaaaattac atcgaattct aatgggggat catatgttta
     5101 accgaccgaa ccgcaacgac gttgatgatg gcgtgcagga tatccagaat gatgtcaatc
     5161 aattagctga cagcctcgaa tctgtattga agtcctgggg cagcgacgcc aaaggggaag
     5221 ctgaagccgc acgcagtaaa gctcaggcgt tgctgaaaga aaccagggcg cgaatgcatg
     5281 gtcgtactcg cgtccagcaa gccgcgcgcg atgccgttgg ctgcgcggat tcttttgttc
     5341 gtgaaagacc ctggtgtagc gtgggtacag cagctgcggt aggtattttt atcggcgcac
     5401 tgttaagcat gcgcaaatcg tagtgcaaaa atgataataa atacgcgtct ttgaccccga
     5461 agcctgtctt cggggtttct ttttgcctgg tgaatcacaa aaatccccct cccccgtcac
     5521 gctcatatcc agggtaattt cgatcactat ttgctatata ttgtgtggtt gaatcttttt
     5581 tcaactacat ctagtatctc tgtatcaaca gagagacaac ccgacgcgta tcatcgcgcc
     5641 gtatcttcat tttaaacaga aatacgaatc atgcgcataa ctatttatac tcgtaacgat
     5701 tgcgttcagt gccacgccac caaacgggcg atggaaaacc ggggctttga ttttgaaatg
     5761 attaatgtcg atcgcgttcc tgaagcagcg gaagcgttgc gtgctcaggg ctttcgtcag
     5821 ttgccggtag tgattgctgg cgatcttagc tggtctggtt tccgtccgga catgattaac
     5881 cgtctgcatc cagcgccaca cgcggccagt gcatgagcca gctcgtctac ttctccagca
     5941 gctccgaaaa cacgcagcgt tttatcgaac gtttaggtct gcccgcggtg cgcatcccgc
     6001 tcaatgagcg ggaacggatt caggtagacg agccttacat cctgatcgtg ccctcttacg
     6061 gcggcggcgg tacggctggc gcggtgccac gacaggtaat tcgcttttta aacgacgagc
     6121 acaaccgggc gttgcttcgc ggcgttattg cttctggtaa tcgcaacttt ggtgaggcgt
     6181 atggccgcgc cggagatgtg attgcccgga aatgcggcgt gccgtggctg taccgttttg
     6241 aactcatggg tacgcaaagc gatatcgaaa acgttcgtaa aggagtaacc gaattttggc
     6301 aacgacaacc gcagaatgcc tgacgcagga aacgatggat taccacgcgc tgaatgcgat
     6361 gcttaatctt tacgatagcg caggtcgcat tcagttcgat aaagaccgcc aggccgttga
     6421 cgcctttatt gcgacgcatg tgcgtccgaa cagtgtgacc ttcagtagcc agcagcagcg
     6481 cctgaactgg ctggtcaacg aaggttacta tgatgaaagc gttcttaatc gctactctcg
     6541 cgactttgtc attacgctgt ttgcccacgc acacaccagc ggttttcgtt tccagacatt
     6601 cctcggggca tggaagtttt acaccagcta tacgttgaag acattcgacg gtaaacgtta
     6661 tctggaagat tttgccgatc gagtcacgat ggtggcgctg acgctggcac aaggcgatga
     6721 gacgctggcg ttgcaactga ccgatgaaat gctgtcagga cgctttcagc cagccacgcc
     6781 aacattcctc aactgcggta agcagcagcg cggcgaactg gtttcctgtt ttttgctgcg
     6841 tattgaagac aatatggagt cgattggtcg ggcggtaaat tccgcactgc agctgtcgaa
     6901 acgcggcggc ggcgtagcat ttttgctgtc gaatctgcgg gaagcgggcg cgccaattaa
     6961 acgtattgaa aatcaatctt ctggcgtaat tccggtgatg aaaatgttgg aagacgcatt
     7021 ttcctatgcc aaccaactcg gcgcgcgtca gggggcaggt gcagtctatt tacatgctca
     7081 tcatcccgat attctgcgtt ttctcgacac gaaacgggaa aatgccgacg aaaaaatccg
     7141 cattaaaaca ctgtcgctgg gcgtggtgat cccggatatc actttccatc tggcaaaaga
     7201 gaatgcgcag atggcgctgt tttcgcccta tgacgttgag cgagtttatg gcaagccgtt
     7261 tgccgatatc gccattagcg aacactatga cgaactggtt gccgatgaac gcatccgcaa
     7321 aaaatacctc aacgcccgtg atttcttcca gcgactggca gaaatccagt ttgagtccgg
     7381 ctatccctac atcatgtatg aagacacagt aaaccgtgct aaccctatcg ccgggcgcat
     7441 aaatatgagt aatctctgct cagaaatttt gcaggttaac agcgcctcag agtatgacga
     7501 gaatctcgac tatgcccgca caggccatga tatttcctgc aatttaggtt cgttgaatat
     7561 tgcgcacacc atggattccc ccgatttttc ccgcacggta gagactgccg tgcgcggttt
     7621 aacggcggtg tcagatatga gtcatatccg cagcgtgccg tccatcgaag ccggaaatgc
     7681 cgcctcgcac gccatcggac tggggcagat gaatttacat ggttatctgg cgagggaagg
     7741 catcgcttat ggttcgccgg aagccctgga tttcaccaat ctctatttct ataccatcac
     7801 ctggcacgca ctgcgtacct cgatgttgct ggcgcgcgaa cgcggtgaaa ccttcgccgg
     7861 gttcaaacag tcacgctatg ccagtggtga atatttcagc caatatctgc aaggtaactg
     7921 gcagccgaaa acggcgaaag ttggcgaact gtttgcccgc agcggtatta cgttacctac
     7981 ccgtgagatg tgggcgcaac tgcgcgacga cgtgatgcgc tacggcatat acaaccagaa
     8041 tctccaggcg gtgccgccaa ccgggtctat ctcttatatc aaccatgcta cgtcgagtat
     8101 tcatccgatt gtggcgaaag tagagatacg caaagagggc aaaacaggac gcgtttacta
     8161 ccctgccccg tttatgacta acgaaaatct ggcgctgtat caggacgctt acgaaattgg
     8221 cgcagaaaag atcatcgaca cctacgcgga agcgactcgc catgtcgatc aggggctgtc
     8281 gctgacgctg tttttccccg ataccgccac cactcgcgat atcaacaaag cgcagattta
     8341 cgcctggcgc aaaggtatca aaacgctcta ttacatccgc ctgcgtcaga tggcgctgga
     8401 aggcactgaa attgaaggct gcgtctcctg tgcactttaa ggaatatcta tgaaactctc
     8461 acgtatcagc gccatcaact ggaacaagat atctgacgat aaagatctgg aggtgtggaa
     8521 tcgcctgacc agcaatttct ggctaccaga aaaggtgccg ctgtcgaacg atattcccgc
     8581 ctggcagaca ttaactgtcg tagaacaaca actgacgatg cgcgttttta ctggcctgac
     8641 gctgctcgac acgctgcaaa atgttatcgg cgcgccttcg ctgatgcccg atgcgctcac
     8701 gcctcatgaa gaggcggtat tatcgaatat cagctttatg gaagcggttc atgcccgctc
     8761 ttacagttcg attttctcga cgctgtgcca gaccaaagat gtcgatgccg cctacgcctg
     8821 gagtgaagaa aacgcaccgt tgcagcgaaa agctcagatt attcagcaac attatcgcgg
     8881 tgatgatccg ctgaaaaaga aaatcgccag tgtgtttctt gaatcttttt tgttctattc
     8941 cggtttctgg ctgccgatgt atttttccag ccgcggaaag ctgaccaata ccgcggacct
     9001 gatccgtctg attatccgcg atgaagcagt ccacggttac tacataggct ataaatatca
     9061 gaaaaacatg gaaaagatat ctctgggaca acgtgaagag ttgaagagtt tcgccttcga
     9121 tttgttgctg gaactctacg acaacgagtt gcaatacacc gatgagctgt acgccgaaac
     9181 cccgtgggct gacgatgtga aagcgtttct ctgttacaac accaataagg ctttgatgaa
     9241 tctgggctac gaaccgttat ttcccgcaga aatggcggaa gtgaatccgg caatcctcgc
     9301 cgcgctttcg ccgaatgccg atgaaaatca cgatttcttt tccggttcag gctcctctta
     9361 tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag ggtgttattt
     9421 tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt tttatcacgc
     9481 aaatattctg tagtgatcta cactgatact ctgttgcatt attcgcctga aactacaata
     9541 ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat ttgctctcag
     9601 cccttatatc acggaaaatt ccggcgattt gctcgcatca atattcatgc cacatttgcc
     9661 ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact attcccattg
     9721 ggtaatatat cgacataaac aaataaagga atcattctat tgcatggcca ttaaattaga
     9781 aattaaaaat ctttataaaa tatttggtga gcatccacag cgagcgttca aatatatcga
     9841 acaaggactt tcaaaagaac aaattctgga aaaaactggg ctatcgcttg gcgtaaaaga
     9901 cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat ccggctcggg
     9961 taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg ggcaagtgct
    10021 gattgatggt gtggatattg ccaaaatatc cgacgccgaa ctccgtgagg tgcgcagaaa
    10081 aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg tgctggacaa
    10141 tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg aaaaagccct
    10201 tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg atgaactctc
    10261 tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc cggatatatt
    10321 attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga tgcaggatga
    10381 gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc acgatcttga
    10441 tgaagccatg cgtattggcg accgaattgc cattatgcaa aatggtgaag tggtacaggt
    10501 cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta ccttcttccg
    10561 tggcgttgat attagtcagg tgttcagtgc gaaagatatt gcccgccgga caccgaatgg
    10621 cttaattcgt aaaactcctg gcttcggccc acgttcggca ctgaaattat tgcaggatga
    10681 agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg cagtctccat
    10741 cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc tgattgatgc
    10801 gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg tcggacaggc
    10861 accctgtgcg gtgcccgtgg tcgacgagga ccaacagtat gtcggcatca tttcgaaagg
    10921 aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa aataatccgt
    10981 gggataccac gccagcggcg gacagtgccg cgcaatccgc agacgcctgg gggacaccga
    11041 cgactgcacc ggctgacggc ggtggtgctg actggctgac cagtacgcct gcgccaaacg
    11101 tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc gacagttggg
    11161 tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag ggcgtgcgcg
    11221 ttccggttga ttatatcctc aacggtttcc agcaattgct gctgggtatg ccagcgccag
    11281 tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga atgggcgtgg
    11341 cgacgctggt ctcgctgatt gccatcggcg caatcggtgc ctgttcgcag gcaatggtga
    11401 ctctggcgct ggtgttaact gccctgctgt tctgtatcgt catcggtttg ccgttgggga
    11461 tatggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt gatgccatgc
    11521 agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt atcggtaacg
    11581 tgccgggcgt ggtggtgacg atcatctttg cgctgccgcc gattatccgt ctgaccattc
    11641 tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc ggtgccagcc
    11701 cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgaccatt atggcgggcg
    11761 ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg attgccgtcg
    11821 gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg cttgccaccg
    11881 ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg caggccgttg
    11941 ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct gttggtctgc
    12001 tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc attatcaccc
    12061 tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg tttgccacgc
    12121 ttatctctac acaaactttt gctgccgatc tgccgggcaa aggcattact gttaatccag
    12181 ttcagagcac catcactgaa gaaaccttcc agacgctgct ggtcagtcgt gcgctggaga
    12241 aattaggtta taccgtcaac aaacccagcg aagtggatta caacgttggc tacacctcgc
    12301 ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactccat gacaacatgt
    12361 acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcag
    12421 cacagggtta cctgatcgat aagaaaaccg ccgaccaata caaaatcacc aacatcgcac
    12481 aactgaaaga tccgaagatc gccaaactgt tcgataccaa cggcgacggt aaagcggatt
    12541 taaccggttg taaccctggc tggggctgcg aaggtgcgat caaccaccag cttgccgcgt
    12601 atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg atggccgaca
    12661 ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg ccgtactggg
    12721 tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg ttctccgcac
    12781 tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac ggcttcccgg
    12841 tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg gcggcagcca
    12901 aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac gccattatgc
    12961 atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg atcaaagccc
    13021 accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgcagaag taatttttat
    13081 tcgggcggat aaggcgtttt cgcctcatcc gccgctctgt acacaatgcc tgatgcgacg
    13141 ctgacgcgtc ctatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc
    13201 ccatattcat caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa
    13261 aataatgact aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc
    13321 caccggtctg gcggtagcca gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg
    13381 taacttttcc ctttccgcca gttcggcagg ctttattgtt accgccgcgc agttgggcta
    13441 tgccgcaggt ctactgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat
    13501 tgtctcgatg accttactgg cagcgggcgg tatgttaatc actgccagca gtcagtcact
    13561 ggcgatgatg atcctcggta cggcattaac cggtttattc tcagtcgtgg cacaaattct
    13621 ggttccgctg gcagcgacgc tggcttcacc ggataaacgc ggcaaagtgg ttggcaccat
    13681 tatgagcggt ctgctgttgg ggatattgct ggcacgaacc gttgccgggt tactggcaaa
    13741 cctcggcggc tggcgcaccg tcttttgggt tgcttcggtg ttaatggcac tgatggcgct
    13801 ggcattatgg cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt
    13861 gttgggttcc gttttcagca tgttcatcag cgataagatc ctgcgcaccc gcgcgttgct
    13921 gggctgcatg acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc
    13981 cgctccacct tttaactaca gcgatggtga cattggtctg tttgggcttg cgggagctgc
    14041 cggagcgttg ggcgctcgtc cggcgggcgg ttttgccgac aagggcaaat cgcaccacaa
    14101 ctttcggtct gcttctgctg ttactttcat ggctggcaat ctggtttggt cacacttccg
    14161 tactggcgtt gattatcggc attctggtgc tggacctcac cgtacagggc gtgcatatca
    14221 ctaaccagac ggtaatttat cgaatacatc ctgatgcgcg taatcgcctg accgcaggtt
    14281 acatgaccag ctactttatt ggcggtgccg ccggttcgct aatttcagcc tcagcctggc
    14341 aacatggcgg ttgggctggc gtttgtctgg ctggcgcgac gattgccctg gttaacttac
    14401 tggtctggtg gcgaggtttt catcgtcagg aagccgcaaa ttaagcaatc agcaaccgtt
    14461 ttcgtggctt tacacttata agggtgttaa gaagcccatc agtctgataa ggttaagata
    14521 ttcattcagt ctatttataa tattaacaat cgttaagcgt acactctatg gaaagcccta
    14581 ctccacagcc tgctcctggt tcggcgacct tcatggaagg atgcaaagac agtttaccga
    14641 ttgttattag ttatattccg gtggcctttg cgttcggtct gaatgcgacc cgtctgggat
    14701 tctctcctct cgaaagcgtt tttttctcct gcatcattta tgcaggcgcg agccagttcg
    14761 tcattaccgc gatgctggca gccgggagta gtttgtgggt tgctgcactg accgtcatgg
    14821 caatggatgt tcgccatgtt ttgtatggcc cgtcactgcg tagccgtatt attcagcgtc
    14881 tgcaaaaatc gaaaaccgcc ctatgggcgt ttggcctgac ggatgaggtt tttgccgccg
    14941 ccaccgcaaa actggtacgc aataatcgcc gctggagcga gaactggatg atcggcattg
    15001 ccttcagttc atggtcatcg tgggtatttg gtacggtaat aggggcattc tccggcagcg
    15061 gcttgctgca aggttatccc gccgttgaag ctgcattagg ttttatgctt ccggcactct
    15121 ttatgagttt cctgctcgcc tctttccagc gcaaacaatc tctttgcgtt accgcagctt
    15181 tagttggtgc ccttgcaggc gtaacgctat tttctattcc cgtcgccatt ctggcaggca
    15241 ttgtctgtgg ctgcctcact gcgttaatcc aggcattctg gcaaggagcg cccgatgagc
    15301 tatgaggttc tgctgcttgg gttactggtt ggcgcggcga attattgctt ccgctatttg
    15361 ccgctgcgcc tgcgtgtggg taatgcccgc ccaaccaaac gtggcgcggt aggtattttg
    15421 ctcgacacca ttggcatcgc ctcgatatgc gctctgctgg ttgtctctac cgcaccagaa
    15481 gtgatgcacg atacacgccg tttcgtgccc acgctggtcg gcttcgcggt actgggtgcc
    15541 agtttctata aaacacgcag cattatcatc ccaacactgc ttagtgcgct ggcctatggg
    15601 ctcgcctgga aagtgatggc gattatataa cccacaagaa tcatttttct aaaacaatac
    15661 atttacttta tttgtcactg tcgttactat atcgactgaa attaatgagg tcatacccaa
    15721 atggatagtt cgtttacgcc cattgaacaa atgctaaaat ttcgcgccag ccgccacgaa
    15781 gattttcctt atcaggagat ccttctgact cgtctttgca tgcacatgca aagcaagctg
    15841 ctggagaacc gcaataaaat gctgaaggct caggggatta acgagacgtt gtttatggcg
    15901 ttgattacgc tggagtctca ggaaaaccac agtattcagc cttctgaatt aagttgtgct
    15961 cttggatcat cccgtaccaa cgcgacgcgt attgccgatg aactggaaaa acgcggttgg
    16021 atcgaacgtc gtgaaagcga taacgatcgc cgctgcctgc atctgcaatt aacggaaaaa
    16081 ggtcacgagt ttttgcgcga ggttttacca ccgcagcata actgcctgca tcaactctgg
    16141 tccgcgctca gcacaacaga aaaagatcag ctcgagcaaa tcacccgcaa attgctctcc
    16201 cgtctcgacc agatggaaca agacggtgtg gttctcgaag cgatgagcta acgcgtcatc
    16261 tcgctcaaaa atccagattt ataaaagaaa aaatgaccgg ccagcatcgc aacatgctgg
    16321 cctttttggc aagcaggtcg gctcagccga tgagttaaga agatcgtgga gaacaatatg
    16381 agcgcaaatg cggagactca aaccccgcag caaccggtaa agaagagcgg caaacgtaag
    16441 cgtctgctcc tccttctcac cttgctcttt ataattattg ccgtagcgat agggatttat
    16501 tggtttttgg tactgcgtca tttcgaagaa accgatgacg catacgtggc agggaatcag
    16561 attcaaatta tgtctcaggt gtctggcagc gtgacgaaag tctgggccga taacaccgat
    16621 tttgtaaaag aaggcgatgt tctggtcact ctcgacccga cagatgctcg ccaggcgttt
    16681 gaaaaagcca aaactgcact ggcttccagc gttcgccaaa cccaccagct gatgattaac
    16741 agcaaacagt tgcaggcgaa tattgaggtg caaaaaatcg ccctcgcgaa agcacaaagc
    16801 gactacaacc gccgtgtgcc gctgggcaat gccaacctga ttggtcgcga agagctgcaa
    16861 cacgcccgcg acgccgtcac cagtgcccag gcgcaactgg acgtcgctat tcaacaatac
    16921 aatgccaatc aggcgatgat tctggggact aaactggaag atcagcctgc cgtgcaacag
    16981 gctgccaccg aagtacgtaa cgcctagctg gcgctggagc gtacccgtat tgtcagtccg
    17041 atgaccggtt atgtttcccg tcgtgccgtg caaccgggtg cgcaaattag cccaacgacg
    17101 ccgctgatgg cggtcgttcc agccaccaat atgtgggtgg atgccaactt taaagagacg
    17161 cagattgcca atatgcgcat cggtcagccg gtcaccatca ccaccgatat ttacggcgat
    17221 gatgtgaaat acaccggtaa agtggttggt ctggatatgg gcacaggtag cgcgttctca
    17281 ctgcttccgg cgcaaaatgc gaccggtaac tggatcaaag tcgttcagcg tctacctgtg
    17341 cgtatcgaac tggaccagaa acagctggag caatacccgc tgcgcattgg tttgtccacg
    17401 ctggtgagcg tcaataccac taaccgtgac ggtcaggtac tggcaaataa agtacgttca
    17461 acaccggtcg cggtaagtac cgcgcgtgaa atcagcctag cacctgtcaa taaactgatc
    17521 gacgatatcg taaaagctaa cgctggctaa tccagaggtg cgtgtgatgc aacagcaaaa
    17581 accgctggaa ggcgcgcaac tggtcattat gacgattgcg ctgtcactgg cgacattcat
    17641 gcaggtgctg gactccacca ttgctaacgt ggcgatcccc actatcgccg ggaatctggg
    17701 ctcatcgctc agccaaggaa cgtgggtaat cacttctttc ggggtggcga atgccatctc
    17761 gatcccgctt accggctggc tggcaaagcg cgtcggggaa gtgaaactgt tcctttggtc
    17821 caccatcgcc tttgctattg cgtcgtgggc gtgtggtgtc tccagcagcc tgaatatgct
    17881 gatcttcttc cgcgtgattc aggggattgt cgccgggccg ttgatcccgc tttcgcaaag
    17941 tctattgctg aataactacc cgccagccaa acgctcgatc gcgctggcgt tgtggtcgat
    18001 gacggtgatt gtcgcgccaa tttgcggccc gatcctcggc ggttatatca gcgataatta
    18061 ccactggggc tggatattct tcatcaacgt gccgattggc gtggcggtgg tgttgatgac
    18121 actgcaaact ctgcgcggac gtgaaacccg caccgaacgg cggcggattg atgccgtggg
    18181 gctggcactg ctggttattg gtatcggcag cctgcagatt atgctcgacc gcggtaaaga
    18241 gctggactgt ttttcatcac aggaaattat cattcttacc gtggtggcgg tggtggctat
    18301 ctgcttcctg attgtctggg agctgaccga cgataacccg atagtcgatc tgtcgttgtt
    18361 taagtcgcgc aacttcacca tcggctgctt gtgtatcagc ctcgcgtata tgctctactt
    18421 cggcgctatt gttctgctgc cgcagttgtt gcaggaggtc tacggttaca cggcgacctg
    18481 ggcaggtttg gcctctgcgc cggtagggat tattccggtg attctgtcgc cgattatcgg
    18541 ccgcttcgcg cataaactgg atatgcggcg gctggtaacc ttcagcttta ttatgtatgc
    18601 cgtctgcttc tactggcgtg cctatacctt tgaaccaggt atggattttg gcgcgtcggc
    18661 ctggccgcag tttatccagg ggtttgcggt ggcctgcttc tttatgccgc tgaccaccat
    18721 tacgctgtct ggtttgccac cggaacgact ggcggcggca tcgagcctct ctaactttac
    18781 gcgaacgctg gcggggtcta tcggcacgtc gataaccacg accatgtgga ccaaccgcga
    18841 gtcgatgcac catgcgcagt tgactgagtc ggtaaacccg ttcaacccga atgcccaggc
    18901 gatgtacagt caactggaag ggcttgggat gacgcaacag caggcatcag gctggattgc
    18961 ccagcagatc accaatcagg ggctgattat ttccgccaat gagatcttct ggatgtcagc
    19021 cgggatattc ctcgtactgc tggggctggt gtggtttgct aaaccgccat ttggcgcagg
    19081 tggcggcgga ggcggtgcgc actaagtaca actaagccag ttcatttgaa ctggcttttt
    19141 tcaattaatt gtgaagatag tttactgact agatgtgcag ttcctgcaac ttctctttcg
    19201 gcagtgccag ttcttcgttg ctgttgatgc gtacgtcacg ttccagaatg ctacgcgcaa
    19261 tatcctgcgc ttcctgcaac gagtgcatct ggtaagtgcc acactggtag acgttcagtt
    19321 ccgggatctg attctgatcc tgcactttca gcacgtcttc cattgccgct ttccaggcat
    19381 cagcaacacg ctgctcatct ggcgtaccaa tcagactcat ataaaaaccg gtgcggcagc
    19441 ccattggcga gatatcgata atctctacac cattaccgtt aagatggtta cgcataaaac
    19501 cagcaaacag gtgctccagg gtatggatcc ctctttctgg catcacttct ttgttcggca
    19561 cgcagaagcg cagatcgaac acggtgattg cgtcgccatg cggggtgttc attgttttcg
    19621 ccacccgaac tgcaggcgct tccatccggg tatgatcgac tgtgaagcta tctaacaacg
    19681 gcatttagcc acctccggta atttttttaa aaattttctg aactctttct tcccaggcga
    19741 gtctgagtat atgaaagacg cgcatttgtt atcatcatcc ctgaattcag agatgaaatt
    19801 ttggccactc acgagtggcc tttttctttt ctgtcaggcg tgtttttcca gccacaccgc
    19861 aaacggttcg gtatcagcgg tttccatttc ctgctgacgg cgttcagatg cctcgcgctc
    19921 ggctacaaaa tcctcttcgc gcagaatttc cagcggctct tcacgcagca gattacggta
    19981 ggcttctgca aacgctttgc ctgttccgcc aataccagta tcaatcatag accttaagat
    20041 acgggcagag aaagtcagat cgggattatc gaagcaggca accagttcat cacacacttt
    20101 ctgatacgct tcgccgccgt taatgctatc cagcgtttgc gcgacgcgtt tcagatcgtg
    20161 gaacagatct ttacccacct gcagtaacgg gaactgcgcg gtttcgcagc cgatacccag
    20221 cgtcaggccc ggtttgcgac cttcaaggat cacccggttc cagttaacgc gcgtacaggc
    20281 aagttcgcta ctgctcattt ccggtgcatc agccagcgca caccagacca taaacaggtc
    20341 gaggaatcgc acctgctgtt catctacacc aatcggcgag aacgggttga tgtccagcga
    20401 acgcacttca atatattcaa tgccgccacg taacagcgca tcagaaggtg actcgccgct
    20461 gcgggtaacg cgttttggac gaatcggcgc atacagttcg ttttcaatct gcagcacgtt
    20521 gctattgatt tgcagcctct taccgtcttt ctcaatacca atcttcgcgt actcttccga
    20581 tggcgttttg attgcctgtt taaggcccgc tacgtactcg taaagatcgt tgaaggtaat
    20641 accaagattg ctttgcgatt tattggtata gccgagatcg ctcaaacgaa gagaggtcgc
    20701 atacggcagg taatacatac cgcactcggt tttctcaaac ggcagcgacg ttggttttcc
    20761 ttgcaggaaa gaagaacaaa tcgccggaga tgcaccaaac agataaggaa tgacccaacc
    20821 gaaacgatag taattgcgga taacgcggaa atagcccgca gaaattttct ctttggcatc
    20881 agcgcccgag atatcaccgc acttcgcttg ccagaatgcc attggcaaag agaaattgta
    20941 gtgcacgccg gaaatggttt gcatcagcgc gccgtagcga tttttcagcc cttcacgata
    21001 cagcgtttta aagcgtccgg tgttagaagt gccgtactgt gccagttcga tgtcctgacc
    21061 ttctgcgatg tagcatggca tacttaacgg ccacatccgc tcatcgccca tattgcgcgc
    21121 cgtataacga tgcagatcgc gcataaaggt cagcatatgt tcaatatcac catccactgg
    21181 tgtaatgaat tccagcaatg cttccgcaaa atcggtagta atccatttgt gcgtcagtgc
    21241 ggaacctaat gcttcaggat gacctgttgt tgccagtgtg ccatcagcat taacacgcaa
    21301 agtttcgcgc tccagcccac gctgtatccc ctttaacgcc tgaggatgtt tttccagcca
    21361 ggccagcgcc tgtgatacgt ccgggatcaa attgacctcc cgcctgtcaa aatcgtttta
    21421 attagcataa ctgtaatggt gaccatatgt gcaggcctac aattagtgcc accacatcat
    21481 gccctgaacg gtcgctgctg caactgcaac atagcgtaac gctttaccaa ggcacaaaaa
    21541 aaagattact ggcccccagg agatgcgcat ccatcccgct aacagacaca gtaaatcgcc
    21601 aaccacgggc atccagctta ataatagcgt gactgcacca tagcgtttca gccagccggt
    21661 agctttctct tgccagcgcg atgttttacg caatggaaag aaacgcccaa ggataacgtt
    21721 agttaaccct ccaaggctat tacccattgt tgctgttaaa actaaaaccc agggatgact
    21781 gatcccggaa agcaacattg ccaccagcac gacttcggag ttgccgggta atagtgtagc
    21841 gctgagaaaa ctactggcaa acaacgagaa aagcgataac gcttcactca cagcaagcga
    21901 acatccacag cgtccatgcc tgctgcacgg gccgcctgaa taccgaaatc ggcatcttca
    21961 aagaccacac actgcgtcgg ttgcacgccc atacgctgcg cgcacaacaa aaatgtgtct
    22021 ggcgcgggtt tatggtgttt gacgtgatca gcagcgacga cggcgtcaaa ataacggcgt
    22081 aatcccaggt gcgccagcaa tgcctctgcg atggcgcttt cactccccgt tcctacagcc
    22141 attgggcgac gaccatgcca acttttcacc acttcaacaa gaggaagcgg ctcgacacta
    22201 tccagcagca tacttcttac tgcttctgtt ttttcacgcg ctaacgcatg cgggtcgaga
    22261 tcggcctgat tcagctcaat aattgcctga gcaatacgcc aggtgggcga tccattaagc
    22321 gcaatcatcg cctgaatatc gtactgaaga ccgtagtgcc ctaatacttc gcgccacgct
    22381 ttacggtgcg taggctccgt atccaggatt gtgccatcca tatcaaaaat taaacctgca
    22441 taacgctcgt acatggtctt ctcgcaagtc gaaaaatatg acgctacttt agcgtaattg
    22501 cttgattttg tcgctgatgg tgaaggagaa gaaaattcag gaaatggata aagtagttat
    22561 ttagaaatga gatatttttg aaggaaattt tttggagaa
//
LOCUS       DADMFL010000061        22493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.061, whole genome shotgun sequence.
ACCESSION   DADMFL010000061 DADMFL010000000
VERSION     DADMFL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.061"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(101..2956)
                     /gene="valS"
                     /locus_tag="JNZ27_002377"
     CDS             complement(101..2956)
                     /gene="valS"
                     /locus_tag="JNZ27_002377"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY8664688.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(2956..3399)
                     /gene="holC"
                     /locus_tag="JNZ27_002378"
     CDS             complement(2956..3399)
                     /gene="holC"
                     /locus_tag="JNZ27_002378"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY8664689.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3533..5044)
                     /gene="pepA"
                     /locus_tag="JNZ27_002379"
     CDS             complement(3533..5044)
                     /gene="pepA"
                     /locus_tag="JNZ27_002379"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY8664690.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKTGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5311..6411
                     /gene="lptF"
                     /locus_tag="JNZ27_002380"
     CDS             5311..6411
                     /gene="lptF"
                     /locus_tag="JNZ27_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8664691.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6411..7493
                     /gene="lptG"
                     /locus_tag="JNZ27_002381"
     CDS             6411..7493
                     /gene="lptG"
                     /locus_tag="JNZ27_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8664692.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7601..9115)
                     /locus_tag="JNZ27_002382"
     CDS             complement(7601..9115)
                     /locus_tag="JNZ27_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY8664693.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(9244..10263)
                     /gene="ahr"
                     /locus_tag="JNZ27_002383"
     CDS             complement(9244..10263)
                     /gene="ahr"
                     /locus_tag="JNZ27_002383"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY8664694.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            10459..10543
                     /locus_tag="JNZ27_002384"
     tRNA            10459..10543
                     /locus_tag="JNZ27_002384"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10493..10495,aa:Leu,seq:caa)
     gene            10730..11995
                     /locus_tag="JNZ27_002385"
     CDS             10730..11995
                     /locus_tag="JNZ27_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8664695.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
     gene            complement(12396..12841)
                     /locus_tag="JNZ27_002386"
                     /pseudo
     CDS             complement(12396..12841)
                     /locus_tag="JNZ27_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13422..13651
                     /locus_tag="JNZ27_002387"
                     /pseudo
     CDS             13422..13651
                     /locus_tag="JNZ27_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            14682..15899
                     /locus_tag="JNZ27_002388"
     CDS             14682..15899
                     /locus_tag="JNZ27_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664696.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIIIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFVFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            15911..17029
                     /locus_tag="JNZ27_002389"
     CDS             15911..17029
                     /locus_tag="JNZ27_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8664697.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            17072..17197
                     /locus_tag="JNZ27_002390"
     CDS             17072..17197
                     /locus_tag="JNZ27_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664698.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPRMAS"
     gene            complement(17250..17505)
                     /locus_tag="JNZ27_002391"
                     /pseudo
     CDS             complement(17250..17505)
                     /locus_tag="JNZ27_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17495..17731
                     /locus_tag="JNZ27_002392"
                     /pseudo
     CDS             17495..17731
                     /locus_tag="JNZ27_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17819..18057
                     /locus_tag="JNZ27_002393"
                     /pseudo
     CDS             17819..18057
                     /locus_tag="JNZ27_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            18298..19629
                     /locus_tag="JNZ27_002394"
                     /pseudo
     CDS             18298..19629
                     /locus_tag="JNZ27_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375825.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            19643..20511
                     /locus_tag="JNZ27_002395"
                     /pseudo
     CDS             19643..20511
                     /locus_tag="JNZ27_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(20740..21753)
                     /locus_tag="JNZ27_002396"
     CDS             complement(20740..21753)
                     /locus_tag="JNZ27_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HAY8664699.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(21765..>22493)
                     /locus_tag="JNZ27_002397"
     CDS             complement(21765..>22493)
                     /locus_tag="JNZ27_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664700.1"
                     /translation="MLENTLEPYKYMIMILVVVMVLFLLTRFPTCKVAQTSHYKRPSA
                     MDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIRLALELGDINERDASNFMVYSFA
                     CFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYVALAPSFSAVYVAVLVSVLFGPC
                     WATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPAIQGYIADMFHSLQLSFLVSMLC
                     FVYVGVYFWRESKVRTALAEVTAS"
BASE COUNT         5666 a         5451 c         5533 g         5843 t
ORIGIN      
        1 tagccagtat ttctcgggga gttatgcctt ctttaccatt tctgtaagag ataaaaaagg
       61 ccggagcatg ctccggcctt cgttttcatc actgtgttga ttacagtgcg gcgataacag
      121 cctgctgttc aatcagcttg gctttcgctt ctgcataacc ttccagcttc tcacgctctt
      181 tcgcgataac cgcttccggt gcgcgggcga caaagccttc atttgccagc ttgttctcga
      241 tgcggctgat ttcaccttcg atcttcgcca cttctttcgc caggcgcgcc agctcatctt
      301 ctttgttgat gaggccagcc atcgggatca gcagctctgc gccgtcgatg atcttggtaa
      361 cggaaaccgg acctttgtca tcggcaggca gcacggtgat gctttccaga cgcgccagcg
      421 tttgcaggaa gccacggttt tcatttacgc gacgttctgc atccgcgctg caaccacgca
      481 gcagcagctc cagcggtttg cccggcgcga tgttcatttc tgcacggatg ttacgtacag
      541 caacgatcgc ctgtttcagc cactcggtgt cggccagtgc ggcttcatca acctgagatg
      601 catcatactg cgggaacggc tgcagcatga tggtgtcggc agtgataccg caaagtactt
      661 tcacacgctg ccagatggtt tcggtgatga acggaatgat cggatgcgcg aggcgcagca
      721 gaccttccag tacagtcacc agcgtatggc gagtaccgcg cagttcggct tctgttccac
      781 cgttcattac cggcttggtc agctccagat accagtcgca gaactggttc caggtgaact
      841 catacaaaat gcctgcggcg atatcgaagc ggaagctgtc cagcgcttcg cggtacgctt
      901 tgatagtctg gttgaactcc gccagaatcc agcggtccgc cagcgatagc gtcatttcgc
      961 cgccgttgaa gccgcaatcc tgaccttctg tgttcatcag cacaaagcgg ctggcgttcc
     1021 acagcttgtt acagaagtta cggtagcctt ccagacgctt catatcccag ttgatgtcac
     1081 gaccggtaga cgccagcgcc gccagggtga agcgcagggc atcggtgccg tgcggctcaa
     1141 tgccgttcgg gaactgtttc tcggtgcgct tacggatttt gtccgccagc tgcggctgca
     1201 tcatattgcc ggtgcgtttt tccagcagtt ctggcagcga aataccgtca accatatcca
     1261 gcgggtcgat aacgttaccc ttggatttgg acatcttctg accttcgtcg tcacgaatca
     1321 gaccggtcat gtaaacagtg tggaacggca cctgcggttt gccattttca tctttgatga
     1381 agtgcatggt catcatgatc atacgggcaa tccagaagaa gatgatgtcg aaaccagata
     1441 ccatcacgct ggttgggtgg aactgacgca gggcgtcggt attttccggc cagccaaggg
     1501 tagagaaggt ccacagtgca gaagagaacc aggtgtcgag aacgtcttcg tcctgacgca
     1561 gggcaacgtt ggcaccgagg ttattttcct tgcgcacttc ttcttcgttg cggccaacat
     1621 aaacattacc cgcttcgtca taccatgccg ggatacggtg accccaccac agctgacgag
     1681 agatacacca gtcctgaata tcgcgcatcc aggagaagta catgttttcg tactgcttcg
     1741 gtacgaactg aatgtcgccg ttctcaaccg cttcaactgc cggtttcgcc agtacatcgg
     1801 cacgcacgta ccactggtcg gtcagcatcg gttcgataac cacgccgcca cggtcgccgt
     1861 aaggaacggt caggtcgtgc ggtttaattt cttccagcag gccgagtgca tcaaccgcag
     1921 caacgactgc tttacgtgca gcaaaacgct cgagtttctg gaactctgcc gggatctcgc
     1981 tggaataaac gtcagattcg ttacctttgg tatcgaacac ctgggcgctt tcacggatat
     2041 caccgtcaaa ggtcaggatg ttgatcatcg gcagggcgtg acgtttaccg acttcatagt
     2101 cgttaaagtc gtgcgccggg gtgatcttca cgcagccggt gcctttttcc atgtcggcgt
     2161 gttcgtcgcc aacgatcgga atacgacggt taaccagcgg cagaatgaca tatttgccaa
     2221 tcagatcttt gtaacgcgga tcttccgggt taacagctac accggtatcg cccagcaggg
     2281 tttccgggcg agtggtcgca accaccagat aatctttacc gtctgcggtt ttcgcgccgt
     2341 cagccagcgg atagcggatg tgccacatcg aacctttcga ttcgcggttt tccacttcca
     2401 ggtcagaaat agcggtacgc agtttcgggt cccagtttac caggcgcttg ccacggtaaa
     2461 tcaggtcttc tttatacaga cgaacgaaaa cttctttcac cgcattggac aggccttcgt
     2521 ccatggtgaa gcgttcacgc tcccagtcga cggagttgcc gagacggcgc atctgacggg
     2581 taatggtgcc gccagattcc gctttccatt cccagatttt gtcgatgaaa gcttcgcggc
     2641 cgtagtcgtg acgggtttta ccttcttctg cggcaatctt gcgctcaacg accatctggg
     2701 tagcgatccc ggcgtggtca gtaccgacct gccacagggt gtttttgccc tgcatgcgct
     2761 gatagcggat catggtatcc atgatggttt gctggaaggc gtgacccata tgcaaactgc
     2821 cggtgacgtt cggcggcggg atcatgatgc agaaactttc ctggctttca tcgccattag
     2881 gcttaaagta gccctgcttt tcccagtgct cgtaaagcgg ctgttcgata tcttgtgggt
     2941 tgtatgtctt ttccattatt tccaggttgc cgtattcagg ttgaaaccag ccacgcggta
     3001 ggctttatag cgttcgcgcg ccagttgttt cagagaatct tcataaggaa cgaagtctac
     3061 cacttctgtg aaagcggtgg caaaatctgc aaagcttgtt cgcagactaa tcaatatatc
     3121 gcgcgggctg ctgctgcgtt tttgcggcca ggcgatctcc accggcgcac cgccgcgcgg
     3181 tccttctccc gctaaattat gcggaacaaa gctttctgcc ggacgcgccc acagggcttc
     3241 atccagccgg taagcctgct tttcatcttc acaggcgatg agtacgcgct taccgctgcg
     3301 ccaacgttct gcggcaattt cacacaccag ttgctcaacg gcgcttaagc catcgacggt
     3361 ggtgtcattg tccagaaggt agaacgttgc gtttttcata tatggggctt cttgtggtgg
     3421 atttaaatat tgctgcattc ctcaccctga aagatgagca atgaatttct attgcgctcg
     3481 atcggttccc tctcccttag ggagagggtt agggtgaggg taaaacgcaa acttactctt
     3541 cgccgttaaa cccagcgcgg tttaacagga actgtgccag caacgctacc ggacgaccgg
     3601 ttgcgccttt tgctttacca gaacgccagg cggttcctgc aatatccagg tgcgcccagt
     3661 tatacttacg ggtaaagcgt gacaggaagc aacctgcggt aatcgcccca ccaggacgac
     3721 cgccaatgtt cgccatatcg gcaaaattgg actccagctg ttcctgatac tcgtcaccca
     3781 gcggtaagcg ccatgcgcgg tcaccggatt gttcagacgc ggcaatcagt tcatgggcca
     3841 gcggattatg gttcgccatc agaccagtaa tatgatgacc cagcgcgatc acgcaggcac
     3901 cggtcagcgt cgccacgtca atcactgctt ccggctcaaa acgttcaacg taagttaaca
     3961 cgtcgcacag taccaggcgg ccttcggcat cggtgttcag cacttcaacg gtttgaccgg
     4021 acatggtggt taacacatcg cccggacgat aggcacgccc gccaggcatg ttttcacagc
     4081 ctgccagcac gccaataacg ttaatcggca gttgcagctc cgcgaccata cgcatcacgc
     4141 cgtaaaccgc cgccgcgccg cacatatcgt acttcatctc atccatgcct tctgaaggct
     4201 tgatggagat accgccggag tcgaaggtta aacctttacc caccagcacg attgggcgtg
     4261 catcttccga cgcgttgcct ttgtactcaa tcaccgacat cagcgattcg ttttgtgaac
     4321 cctgaccgac cgccagataa gagtgcattc ccagctcttt catctgctgt tcgccgataa
     4381 cgcgggtgat gacattcttg ctgtagctgt cagccagctg gcgcgcctga gaggcgagat
     4441 aagcggcgtt acagatattc ggcggcatat taccgagatc tttcgctgct tttatcccgg
     4501 cggcaatcgc cagaccgtgc tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg
     4561 gcacgttgaa caccatttta cgcagcggac gacgcggttc gctcttattc gttttcaact
     4621 gatcgaaact gtagagcgtc tcttttgctg tctcgacagc ctgacgcact ttccagtagt
     4681 tgttacggcc tttaacgtgc agttcagtca gaaagcagac cgcttccatc gagccagtat
     4741 cattcagcgt attaatggtt ttctgaataa cctgcttgta ctgacgctca tccagctcac
     4801 gttctttgcc gcaaccaata aggagaattc gctcggaaag tacattcgga acatggtgca
     4861 gcaacaatgt ctgccccgtt tttccttcca gttcgccccg gcgtagcagg gcgctgatgt
     4921 acccatcgct gattttatcg agctgttctg caatcggaga aaggcgacgt ggttcgaaga
     4981 cgcccacgac gatgcaggca ctccgctgtt tctccgggct accgcttttt acactaaact
     5041 ccatgcacta cgctcctgaa tcttaaagac aacggcggtg gctacagata gaattgcaag
     5101 ctttcgtaac tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg ttattacggc
     5161 attggcacgt cagaacaaaa tctgagaggt gaatccgttg agtataatta tcttagcgac
     5221 gatttcgacg actcaagaga ataaatgacg tttaagccat gaaacaagct aaaatcctgc
     5281 aaaagacgag tttttacggg cgtatttaaa gtgataatca taagatatct ggtgcgggag
     5341 acgctcaaaa gccagctggc gatactcttc atcttgcttt tgatcttctt ctgtcaaaag
     5401 ttagtgagga tcctcggcgc agcggttgac ggcgatattc cggcgaatct ggtgctctcc
     5461 cttctcgggt tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag cctgttcctc
     5521 gggctgctga tgacgctggg caaactgtat accgaaagtg aaattacggt aatgcatgcc
     5581 tgcggcttga gcaaagcggt tctggtgaaa gcggcaatga tccttgcggt attcacggca
     5641 atcgtcgcgg cggttaacgt gatgtgggcg ggaccgtggt catcgcgtca tcaggatgaa
     5701 gtgttagcag aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg gcaattccag
     5761 caagcgacta atggcagctc ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa
     5821 gatgtgttcc tcgcacaaat tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc
     5881 gattccggac atttaaccca gctgcgcgac ggctcccagg tcgtcactct caaccaggga
     5941 acgcgcttcg aaggcactgc actgttacgt gatttccgca ttacggattt ccaggattat
     6001 caggcgatca ttggtcacca ggcggtggcg ctcgacccga acgataccga ccagatggac
     6061 atgcgcacat tgtggaacac tgacaccgat cgtgctcgcg cagaactgaa ctggcgtatc
     6121 acgttggtat tcaccgtgtt tatgatggca cttatggttg taccgttgag cgtggttaac
     6181 ccgcgtcagg gacgcgtact gtcgatgctg ccagccatgc tgctgtatct acttttcttc
     6241 ctgatccaga cctccctgaa atcgaacggc ggtaaaggta agctggaccc gacactgtgg
     6301 atgtggaccg ttaacctgat ttatctggct ttggcgattg ttctcaacct ttgggacacc
     6361 gtgccggtcc gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt
     6421 ttggcgtact tgaccgctat atcggtaaaa ctattttcac caccatcatg atgacgctgt
     6481 tcatgctggt gtcgctgtcg ggcattatca aatttgtcga tcagctgaaa aaagccgggc
     6541 aggggagtta cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg
     6601 tgcagatctt cttcccgatg gcggctcttc ttggggcgtt gcttggtctt gggatgctgg
     6661 cgcagcgcag cgaactggtg gtgatgcagg cttctggttt tacccgtatg caggtagcgc
     6721 tgtcggtgat gaaaaccgcc attccactgg tcttgctgac gatggcgatt ggcgaatggg
     6781 tcgcgccgca gggcgaacag atggcgcgta actaccgtgc gcaggcgatg tacggcggct
     6841 cgttgctctc tacccagcaa ggtttatggg cgaaagatgg caacaacttc gtctacattg
     6901 agcgggttaa aggtgacgag gtgttaggtg gcatcagtat ttatgccttt aacgagaatc
     6961 gtcgtctgca atccgtacgt tatgccgcta ccgcgaagtt tgacccggaa cataaagtct
     7021 ggcgtctgtc gcaggttgat gaatctgatc tgaccaatcc gaaacagatt accggttcgc
     7081 agacggtgag cggcacctgg aaaaccgacc tcacgccaga caaactgggc gtggtggcgc
     7141 tggacccgga tgcactctcc atcagtggtt tgcacaacta cgtgaagtat ctgaagtcga
     7201 gcggtcagga tgccggacgt tatcagctca acatgtggag caaaatcttc cagccgctat
     7261 ccgtggcggt gatgatgctg atggcgctgt cgtttatctt tggcccactg cgtagcgtac
     7321 cgatgggcgt gcgtgtggtc acaggtatca gcttcggttt tgtcttctac gtactggacc
     7381 agatcttcgg cccgctgacg ttggtttacg gcatcccgcc gatcatcggc gcactgttgc
     7441 caagtgccag cttcttctta atcagcctgt ggctgttaat gagaaaatcg taatctaaca
     7501 agggagcgaa agctcccttt attattgttg acaaagtcaa atacgagttc gaacggtccc
     7561 ctcgcccctt tggggagagg gttagggtga ggggcacaga tcacgtctga cttatctttt
     7621 tctccccccc cagcaaactc cccaacatcc cgcgtacaat ctgattcgtc acctggcgag
     7681 cggcgctttt cgccattgtt tgcaccacac catctttctt cccgccgcgt ggtccggtag
     7741 tgccaaacaa aatatccttc aatccaccaa gaataccgtc atccaccgcc acctctttcc
     7801 ctttcacggg gggattattt tgctgctcgg tactggcctg aaagcctttt tgcagcatct
     7861 cataggcgga ctcgcggtcc acctcatctt catatttgcc atacaccggg gagtgattaa
     7921 tcaggccatt acgctcatct tccgtcaccg gccccattcg cgaacaaggt gcgatcacca
     7981 tcgcccgttc caccacagaa ggacttcctt ttgcatcgag aaacgagatt aacgcctcgc
     8041 cggtccccag ttcctggatt gccttttcgg tatcaaatgc cggattggcc cgcatggttt
     8101 gcgccgctgt cttcactgct ttctgatctt ttggcgtaaa agcccgcaaa gcgtgctgaa
     8161 cacgattacc gagctggccg agcacattat ccggaatatc agacgggttt tgcgaaacga
     8221 accagacgcc cacgcctttt gagcgaataa gccttatcac ctgctcaatc ttatccagca
     8281 gtacctgcgg tgcatcgtta aacagcagat gtgcctcgtc gaagaaaaac accagttttg
     8341 gtttctccag atcgcccgct tccggcaatt gttcatacaa ctctgaaagc atccacagca
     8401 ggctggcggc gtacagtttc ggcatctgat aaagtttctc ggcgctgagg atattgataa
     8461 cgcctttacc gttggcatcg gtgcgcatcc agtctttgat atccagcatc ggctcaccaa
     8521 agaagtgtgc tgcgccttgc tgttccagcg acagtaatcc gcgctggatg gcaccaaccg
     8581 atgcgctact gatatttccg tactgattct ggaaggattt ggcgttatcg ccgatgtact
     8641 gggtaatcgc ccgcaaatct ttaaagtcga gcagcaacaa tccctgatcg tccgcaatgc
     8701 ggaagataat attcagcacg ccagattgca catcgttgag attcaacagt cgcgccagca
     8761 acagcggccc cagatccgaa accgtcgccc gcaccgtgtg gcctttctcg ccaaagatat
     8821 cccacaccac caccggattg gcatgcggtt gccagtcatt gacgccgata tttttaagcc
     8881 ttgtgagcag tttttccgac gccgttcctg cctgcgcgat accggtcaga tcgcctttca
     8941 catcagccat aaacaccggc acgccgattt ccgacaatga ctctgccagt ttttgcagcg
     9001 taacggtttt acccgtcccc gttgcgccag taatcagccc gtgacggtta gccattcccg
     9061 gcagtaaaaa cagttctgtg tccggcgtgc gggcaattaa caggggttcg ctcatgagat
     9121 ttcctccagt ttcctgcctg gagtataggc aacggctgtg cactcttcac cgctaaattc
     9181 agcagattgc gagacgcctt ccaaagagat ttaagtgccg ggcgacagca cccggcaaag
     9241 ggatcagaag tcggctttca ggactactcg gtagcgcgct ttaccatcgc gtacatgctg
     9301 aatggcgtcg ttaattttcg acatcgggaa cagttcggtt gtcggcgcaa ccttgctgcg
     9361 ggcggcaaag cgcatcagct ttcgcagctc ataaggcgtg ccggtagcag aaccagagat
     9421 gctgcggtcg cccgcaatta acgtaaaggc cggaacagac agcggcgtga gaaccgcacc
     9481 gaccgtatgg aaattaccgc cataggtcag cgcctcaaaa taaggctgcc agtcgaggct
     9541 gacgttcaca gtattgataa tgagatcaaa ctgccccgac agggctttca gtgcctgcgg
     9601 atcgcggcta ttcaccactt tatcggcacc catcgccagt acttcctgct ctttcgccgg
     9661 attagaacta aaggccgtca cctcgcatcc cattgcgtgc agaagtttta tagcgatatg
     9721 ccccagcccg ccaataccaa ttaccccaac gcggctggta gcagtgatat ggtgcatcaa
     9781 cagtggttta aagaccgtga taccgccgca caacagcggc ccggcggact cgatatcaat
     9841 attttctggc agtggaatca cccattgcca gtccgcacgc aacttctcgg caaagccacc
     9901 gcgattcata attgtcggca ccgcaccttg ctcgcagttg atctgattac cgctaataca
     9961 ggcgtcgcag tgaccacagc tacgcgccgt ccagccaatc ccgacacgct gaccgacctg
    10021 caaaccttta tcctgcgcgg cgctcccgag tgccaccacg cgcccaatca cctcatgccc
    10081 ggcaaccagc ggatattgtg aaaatcccca ttcgttatcg atcatcgaca gatcggaatg
    10141 acaaatcccg cagtaatcca cctgcacttc aacatcttgt ggcttcagct caccgggatc
    10201 gtactcataa acttccagtt cgccgcccgc ttcttttgcg gcatagcttt ttatcatcga
    10261 catttttggg tccttctctg gtgttgtttg ggaagtgtag agcatggcag ggcggggggc
    10321 ctggagtgtg acaaaggtta catatcgctg agtgtaatgc tgaaaatttc agcacttagc
    10381 gaggtgggtg caagctggcg cttgcatggt ggcgtgcgac aggtataatc cacaacgttt
    10441 tccgcatacc tcttcagtgc cgaagtggcg aaatcggtag acgcagttga ttcaaaatca
    10501 accgtagaaa tacgtgccgg ttcgagtccg gccttcggca ccaaaagtat gtaaatagac
    10561 ctcaactgag gtcttttttt atgcctgaaa tccagtgttt atctatcttt cccgctatat
    10621 taactctctc aaggtcaacc gatatcaacg tacatctacc aacatatgtt ggtacagatg
    10681 atggtatttc cggttcgata atgcttgtac caacagggag ggaatacgca tggcattaac
    10741 agatatcaaa gtcagagcag ccaagccaac ggataagcaa tataagctga ctgatggtgg
    10801 cggtatgcat ctgcttgtcc atccaaatgg ttctaagtac tggcgtttgc agtaccgtta
    10861 tgagggaaag caaaaaatgc tggcacttgg ggtttatcct gaaatcacac tagcggatgc
    10921 cagagtacgt cgtgacgagg cgcgtaacct gcttgcgaat ggcgtcgatc cgggagacaa
    10981 aaagaaaaat gataaggttg aacagagtaa agcacgaacc tttaaagaag tcgcgattga
    11041 gtggcatggc accaataaaa agtggtctga agatcacgcc catcgtgtgc taaaaagtct
    11101 tgaagataat ctttttgcag cgcttggtga acgtaatatc gctgagttaa aaactcgaga
    11161 tttattagca cctattaagg ccgtagaaat gtctggacgt cttgaagtgg ccgctcgtct
    11221 tcagcagcgc actacagcca tcatgcgcta tgcagtgcaa agtgggttaa ttgattataa
    11281 cccggcacaa gagatggctg gggcggttgc ttcctgtaat cgacaacatc gtcccgcgct
    11341 tgaattaaag cgcatccctg agttgcttac aaaaatagat agctatactg gtaggccgct
    11401 aacccgatgg gcgatagaac tcactttgct gatctttatt cggtccagtg agctgcgttt
    11461 tgctcgttgg tcagagatcg atttcgaagc gtctatatgg actatcccac cggagcggga
    11521 gcctattcct ggagtgaaac attcccatag aggctcaaaa atgcgtacaa cgcatctagt
    11581 gcctctttca acgcaagctc ttgcaatttt aaagcagata aaacagtttt atggggccca
    11641 tgacttgata tttattggtg atcacgattc gcacaaaccc atgagtgaga atacggtaaa
    11701 tagtgcgtta cgggtcatgg ggtatgatac aaaagtagag gtttgtggtc atggctttcg
    11761 aacaatggcc tgtagttcat tggtcgaatc aggtttgtgg tctcgtgatg ctgttgaacg
    11821 tcagatgagc cacatggagc gaaattcagt gagggccgcg tatatccata aagcagagca
    11881 tctggaagaa cggcgattga tgctacagtg gtgggccgat tttctggatg taaacagaga
    11941 aagatttatc agtccatttg aatatgcaaa gattaataat ccattaaaac agtaatcatc
    12001 ccgggcaaat gcccgggaat tattctagga ttattttctt tgttaaaaaa gacaaacggt
    12061 attaactgat gtatttacta tttaccgctc cctgcagggg ggattatcag aatccgcggt
    12121 tattcattat ttatatactt tcaataaaaa agtaacctca taaaaaatcc tgtactcaca
    12181 gggtgatgcg ggataactga cgtaaggagt gctgcaggga tttgatggtc ccttgtgctg
    12241 atatgaatac ggatatttta tacagaatac ttttcagtac aagcgactgt attacagtct
    12301 gccatatatg attacctgtg atcaggaaga ataagtggct gaggtttcaa actgtctgcc
    12361 ggtatattcc tctctccctt aaaaaccata acagtttaat tatcttcgtc tgtcagcgga
    12421 ttgaatggcg gtatattttc agtacaaata ccggtcatcc actgaaaaat acctgcgaaa
    12481 tgacgggcac tgatttttct actgacgggc tgatgagacg ttatgtcact gacatataat
    12541 ccctgcgcac actctcacca gtgacaataa caatcgtatc atataacggt gctcccggct
    12601 ggattttcca gttgtcagcc ccgtgctgac tcagttgttc ataacgctgc atttcacgta
    12661 atgtgtcagt tgtccccaca actgttcctt taaccatccg caatggccaa cggtttacag
    12721 agcataatac gaatagccgt agagccagcc agttacggtg gtcgcgatgg tgtgtccgcc
    12781 agaaaatcac cacgaatacc agaatcgcgg cactgaccag aaaatgataa acaggaaaca
    12841 cggggcggtt cactttgctt tctcctgtag tgttttctgt aggttctgaa ccggttcgag
    12901 cggggttgtg aggctgatgg caatccccgg gctcttcctg catgttacac tggtactgca
    12961 agagggggcc ttcgctcctc tgacattact cagtattttc actactacac cccatctgcc
    13021 atccataaca accgattcac cccctcgcga ggcgttgttg cccgtcaccc ggacagacat
    13081 cgttatggac ttctcttgtt gcacgatcga tcaattgtta tgcatgctga caccacaacc
    13141 ccggtgggta tgaccgatgc ctttctcact ctcttcccgg tcatcgtcgg ccttccccgt
    13201 tattgtggag tgtcagcacc ctcgacggca tttcgatggc tgctcagtgt tcactcacgt
    13261 tacggcccgc ataacccgtt gacttcctga agaagtcgtt tcagggagag attcagccct
    13321 tttgttacct cctggaccgc ttcttgtcct tccagtcgga gcgagagtta gccggggcgg
    13381 actttcaccg acggaaagat cgtgcatttc aaggcacaca caacaatatc tgcgagcgtg
    13441 ctatccgtcc ggtcgttatg ggacgaaagg cctggttgtt cgctggttca ctgatggccg
    13501 ggaaccgcgc ggcacagata atgagccttc tgggaaccgc agtctggagc cacatgcttg
    13561 gctgacggac gtcctgacgc gtctgccgga gtggccggag gaaaggttgg ctgagttact
    13621 gcctcttgag ggctttacct tcttcgggta agtgatacct gccgtcaggt gtttgtgcac
    13681 cgggccataa cctgcagttg ggaattgaac gcctgacaac aggaaatgag ctagagcagt
    13741 aaacacgcgg gctgacaatg ggtcagaata tgagtcagcc cggcgttatt cctggggtca
    13801 gtacttcaga tccagcctcc ggtaccagac tagctagccc ctacgcaacc aaataaatca
    13861 accatcgtca gagtgcaatc gttcatatac actgtcggtg atgcgctgaa aaatgcgtta
    13921 atgttgagtt cctcgaaccc ttaccaatta agggacatag ccagatacgc aactggctga
    13981 tgcaagcagt ctaatgtctt tagatccgct ggcctgcggg catgacaatg tcaaacagtc
    14041 aaagattgtg gcgttgacgg tcagtcttgg ggacaaaaaa gctcagtatt ctcgttggca
    14101 gccagataat ttaacagttg atgctgaaac gaaataggca tcttggttct ggtgtttgta
    14161 acaaactatc agctacaaaa atatgctcaa tttgtgacat cagtaacaaa acgcgttttg
    14221 ttatgtggat tgcttgtttt ttgatctggt ataacaggta taaaggtata cagaaaagca
    14281 aagaaatact gcaaaggaaa acagctataa cgtaagctaa agtaataacc tctcagtctt
    14341 tcctcatttg actaagggag ttttattcaa cctgaacgga ctacgaaaat gagcacaatg
    14401 aataagtcaa tttttgaagc agggttgaac tgtgccagtg gtggaatatc tggcgaacat
    14461 tgttttttgt tggtgaccca aaactgtagg tgggtatcag ttttatcttt catagagtga
    14521 aatatgttaa gaagaaatgg aggaaaagat tgtacggatt aggtattgat aacaatcaat
    14581 agtactggcg tatttgaaga caatataatt atttctggat attgttgagg ctccctaata
    14641 tttactttaa gggctatatt agaataacac aggaaacaaa tatggcaaca gcatggtata
    14701 aacaagttaa tccaccacaa cggaaagctc ttttttccgc atggcttgga tatgtatttg
    14761 atggctttga ttttatgatg atattttaca ttcttcatat tataaaagca gatcttggca
    14821 ttacggatat tcaggctact ttaataggga cagtggcctt catagccaga cctattggag
    14881 gtggtttttt tggtgccatg gctgataaat atggtcgtaa gccaatgatg atgtgggcaa
    14941 ttatcattta ttcagtcgga acaggcctta gcggtattgc tacaaactta tatatgctcg
    15001 cagtttgccg ttttattgtt ggcttaggga tgtctggtga atatgcatgt gcttcaactt
    15061 atgcggtaga aagttggcct aaaaatcttc aatctaaagc tagtgctttt ttggtaagtg
    15121 gtttttctgt tggaaatatt attgcggcac aaataatccc tcagtttgct gaagtatatg
    15181 gatggagaaa ctcttttttt ataggcctgt taccagtttt actagttctt tggatcagaa
    15241 aaagtgctcc agaaagtcag gagtggattg aagataaata taaggataaa tcaacttttt
    15301 tgtctgtctt cagaaaacca catctttcaa tctctatgat cgttttcctc gtctgttttt
    15361 gtctatttgg tgcaaactgg ccgataaacg gactacttcc ttcctacctg gcagataatg
    15421 gagttaatac agtggttatt tcaactctga tgacaatagc aggtttagga acactgacag
    15481 gtacaatatt ttttggtttt gttggtgata agattggtgt aaaaaaagcc tttgtagtcg
    15541 gtctaataac ttcatttgtt ttcctttgtc ctcttttttt tatttctgtg aaaaactctt
    15601 ctcttatagg attatgtctc tttggattaa tgtttacaaa tttaggtatt gcagggttgg
    15661 ttccaaaatt tatatatgat tacttcccaa caaaattaag aggattaggg accggtctta
    15721 tttataactt aggggcaact ggaggaatgg ccgcacctgt attagctaca tacatttcag
    15781 gatattatgg cttaggtgtt tcattattca ttgttacggt tgcattctct gccttattaa
    15841 ttttgttagt tggttttgat attccaggta aaatttataa actatccgtg gctaaataat
    15901 tggaggcttt atgattaatt atggcgttgt tggtgttgga tactttggcg ctgaattagc
    15961 tcgttttatg aatatgcatg ataatgcaaa aattacatgt gtatacgatc ctgaaaatgg
    16021 agaaaatatt gcccgtgaat tgcagtgtat caatatgtca agcttggatg ctttagtctc
    16081 aagtaaatta gtcgattgcg tgatcgtagc caccccaaat tatctgcata aagaaccagt
    16141 aattaaagca gcaaagaata agaagcatgt tttttgtgaa aaaccaattg cattaagtta
    16201 tgaagattgt gtggatatgg tcaaagcgtg taaagaagct ggtgtgacct ttatggccgg
    16261 gcatattatg aattttttca atggggttca atatgcacgg aagttaatta aagaaggtgt
    16321 tatcggcgaa atattatcat gtcatactaa gagaaatggc tgggaaaaca aacaagagag
    16381 actttcctgg aaaaagatga aagaacaatc tggtggacat ctatatcatc atatacatga
    16441 gttagattgt gttcagcatt tacttggaga aataccagag acggttacta tgattggtgg
    16501 aaatttggcc cattctggtc caggatttgg caatgaagat gatatgttat ttatgacctt
    16561 ggaattcccg tcaggaaaac tagcaacctt agagtggggg agtgcattta actggccgga
    16621 acattatgtc atcatcaatg gaactaaagg ctctattaaa attgatatgc aagaaacagc
    16681 agggtcactt aggattggcg gtcagacaaa gcattttttg gtccatgaaa cacaagaaga
    16741 agatgatgat cgtcggaaag gcaatatgac ctcagaaatg gatggcgcta tagcatatgg
    16801 tcatccagga aaaaaaacac cattatggct tgccagttta ataagaaagg aaacgttatt
    16861 cctccataat atcctctgtg gtgcaaaacc tgaagaagat tatattgacc ttctcaatgg
    16921 tgaggcggcc atgtcggcga ttgctactgc tgatgctgcc actctttcaa gatcgcagga
    16981 caggaaagtg aaaatcagtg agatcattaa acatacatca gtaatgtaaa aactatacgc
    17041 ctcttgattt aattcaggag gccttttatg tatgatcaag aattttatct tcgataatct
    17101 cataatttta gcagtgccat ttatgataaa aacatccctt aaaaccaatc taatcttttt
    17161 cttcctctgc gtatttgttc cccgtatggc gtcataattt acgctacgta atacgggata
    17221 gttacgatac gcagcgatag cgctaagttt tagttaaaat cccccatcag cgggatatgg
    17281 ggatctctgg gaggttcagg ttttagcatc gcgattaata cgacagacgc tggtgacgat
    17341 gatgccaagt agtggctctt ccgaataagg ttcccactga ccaattggcg cgcgattcgc
    17401 aataacatct cccttcgccc agtattggga aaagtagata cattcaaact gtgtacgctg
    17461 tttcgtctca cagttcacga cattgctggc cgatgagctg accattgggc ctatccgggc
    17521 tgtcccgatg gatattacgc cgaagtatgt gggaattgcc agcggattga tgaacgccgg
    17581 ttccgctgtc gccgacatta tttcacctat cgcctttggc attattatcg ataaaacagg
    17641 caactggagt ttaccattct acggttccgt cgcattgctt gttattggta tattcctgac
    17701 gttcttcatg cgtccagata agtcattgta aaagtcgaat tcagggctga agtggtctac
    17761 tgaatttggc catctgaaca gggtgatatg ctcaccttag aacaacacag gtgcaccaat
    17821 gaaaagaaga aatttcagtc ctgaattcaa acgtgaatcc gctcagttgg ttgtcgatca
    17881 aaattacact gtttctgacg ctgctaaggc tatggatgtt ggtctttcca cgatgacgga
    17941 atggttcaaa caactgcgtg atgagcgtca gggcaaacgc caaaagcctt cctgataaca
    18001 ccggaacaaa tcgaactacg tgagctaaag aaaaagctac aacgaattga aatggaaacg
    18061 aaatattaaa aaactttcca tgtgtgaaac cccgtcccga gtctcagaaa ctctccggta
    18121 aagctggcag tctaatatag cgaaggaagc agtcgtcggc cttttggtgg tatgaccctt
    18181 aaaaaacatg gttcaggggc tgtggcggat ccgacggtgg tagcttgatc ccgttcagga
    18241 aggtgatttc tccgtcgtct cctccttcgt tcattaaaaa tgagcaagga gatttatatg
    18301 aaaaaatcct cccgaatgtc tattgtccat tctcatgctg cgggcgttga tattggggcc
    18361 aaatttcacg ttgtggccgt accgcctgat gctgacgccg agccaataag gacttttcaa
    18421 agtttcaccg gcgaccttca tcgtatgtct gactggctca aaacctgtca tattacaacc
    18481 gtcgccatgg agtccaccgg cgtttactgg ttgcctgctt ttgagatcct tgaagcagcg
    18541 ggttttaata tgatactcgt taatgtcagt gccgcaaaaa atgttcccgg tcgcaagact
    18601 gatgtcaacg acgcacagtg gttgcagaga cgacatgcat ttggtcttct ccgggccagt
    18661 ttcagacctg tacacgacat ttcagtcctc aggtcttatc tgaggcaacg agaacgcttg
    18721 actgaatacc gggctgcgca tattcagcat atgcagaaag cgctgatgca gatgaatgtg
    18781 caattgcatc atgccgtatc ggatatcact ggcgtcaccg gtctctcgat cgtcagagct
    18841 attgtcagcg gtgagcccga tccttcagta ttgatccagt accgtgatgt gagatgtaag
    18901 aagacgccag aagtacttca gcaggcactg acagggaact ggcaacctga gcatcttttt
    18961 gcacttgagc agtcggtggc atttttctat ttttaccagg aaaagatccg tgaacgtgac
    19021 gatcaaatcg aaattcatta atacaactca gcaccgggac tgaaaatcct gaaggagtat
    19081 taccctgcgc cagacatcgt acaaagcaac ccaatcagct ttccttgatg tcagaccgtt
    19141 actctggaaa attaccacgg cagatctgac acaaatacat ggggttgggc cttatctggc
    19201 actaaaattc gttgccgaac gcggtactga catgaatcgc tggcctgatg cgagccattt
    19261 tacctcgtgg ctattttgtc cacgggaaat aagatctccg gtggaaaagt gttgtcctcg
    19321 aaaacgagac gttcatccag ttgaattgct gctgcactca ggctggcagc cacaacgatt
    19381 ggtcggagcg atactgcgtt gggtgctttt tatcgtcgat tatcatcccg tataggtaaa
    19441 gcaaaagcag tcactgcaac ggcaagaaaa ctggcaacac tgttctacaa tgccttgaaa
    19501 tatggtaaaa aatatgtcga caattgtgct gattactatg aagagcgtta ccgtagttgc
    19561 gtactcaatg gacttaaacg aagagtaaaa tcactgggtt attctctcca gcaagaccct
    19621 gaattatgag agtgaaccag gagtttctca ggaaggctac cgcgctcttg atgtcagatt
    19681 cctgaacagt ctcaataatc gggaaactca gagcgcatta tcctgtgttc acactctgtc
    19741 atgtgttcgc agttcatcgc tgcagctaca gatattggaa aacccgtcct gaaaaaacag
    19801 acggcagatg ggctaggtaa ttgacttgca tggcggagct aggagcattg ccacaatggc
    19861 aacccggaga gattaccaga tgggacgctg gcttgctggc aggctcatga aaaaacaggg
    19921 gctggtcaac tgtcagccgc cgccccaccg gtcatgaaca tgtcgctatt cctaactatc
    19981 ttgaaccgca cttcgctgtg acacagctaa atcaagtgtg atgacgtgac gtatatctgg
    20041 ataggtaaac gatggggata cctcgcagta gtacttgctc tgttcgcaat aaagcccggg
    20101 gatgggcaat gtcgttctca ccggacagca gactgactat aaaagcgctg aagatggccg
    20161 aggaaacgcg aggtaaacca gagggagtta tgttccttag caacaggaga gccattacac
    20221 aagtagacag ttcaggcagg tgatgtggcg ttatcggatc aaggagagta tgagtcgtcg
    20281 gggaaactgc tgggatatta gcctaatggt acgtttcttc agaagtctga agaacgagtg
    20341 ggcaccagtg actggttact ttagccttaa cgaaggtgtc catacaataa cagagtatat
    20401 aattgaatat taagtgcact ccggcctcat gaatataacg gtggattacc tccgaacgaa
    20461 tcggaaaata gatactggaa aaaatctgaa attgtgacca ttttttgttg accactacaa
    20521 ataaataaaa gagttgcagt gcttgctcgg taagaaaaca atggaagttg agatcctcaa
    20581 agaagctgtg gagtacacca ctctacagta gttgcgcaaa tttggagtgg acgataatgt
    20641 gatcgtctat aagggcaacg ctatcatagt cttgtcctgg cgggtaaaaa agcgcgctta
    20701 ccttaacgat aagcgcgccg ctgttcaggc cttgagtggt tattcaattc ctgtggtgac
    20761 tgtaaaagtg cgcgtttgct gcggtgcaac ctgaatcagc gtgccattac gttgcgcggc
    20821 aagatacccc tcaggccgac aggttgcagg taatgcaaag gcggctacct gttgctctcc
    20881 gttataaagg atccagcgtg tcacataatt tagttcagca ctgtagaaac gagtaacaaa
    20941 cgtagtgcca tcgggagaga tcatgcgaaa ctctggctga tctgtataag cgtccagttt
    21001 gtctgcaaag aagacaattt ctggatcata aaattccggt tgactcagcg tcgacagaga
    21061 ggcttctccc tgcataatcc gttgattaaa cgccagccac tgagcggtgg gattaacatg
    21121 cgaaggcact gattcacgca atcttaatat ttcgtccggg atattctggc tgaatgtagc
    21181 atttggtata tatgcataat tcatgtggca catatattgt agtggcatat ctacagaagc
    21241 cagattggtt acggccatct taatatcgaa cagtgtagag gatttgtgaa ggaccactgt
    21301 tggttgagcc agataatgat gaccgaaacc cattacatac tcgtaacgcc cgttaaggcg
    21361 taacatatct ccgtctaatt ccagccatgc ttcatccatc gcggcacagg ccatttcacc
    21421 gtgtagcaga tgagtatctt ccgcagatgg gcagccatta gccagcaaac ctgaatgaaa
    21481 agcaaaacag ccataggtct ctatcacctc tgtcgccggt ttaggctggc gaaacatatt
    21541 gcacatggtg aggccgtgtc catcaaattg cgcatcccaa atcatctgcc ccatccaggg
    21601 aagaataatc aaatgtccac gactgtttgc aattttaagc ccctcgacac cgctgtcata
    21661 gcgaaaagac gtgacagtaa aatcactatt ttccagcaag atacgaggtt tctcgccaaa
    21721 aagcgcccgc cacaaattaa tacgcgtact cataacggtt ctcctcagga cgctgtgact
    21781 tcagccagtg cggtacgtac tttgctttca cgccagaagt agactccgac atagacaaag
    21841 cagagcatag aaaccaggaa tgaaagctgt agtgagtgga acatatctgc aatatatccc
    21901 tgaattgccg gaaccaccgc ggcaccgaca atagccataa caatgactgc tcctgccatt
    21961 tctgtatgtt cgttatcaac tgtatccagt gttcctgcat agatcgtcgc ccagcaaggg
    22021 ccaaacaaaa cacttaccag gacggcgaca tagaccgcgc tgaaacttgg agccagtgca
    22081 acatatgcca ggaacagcgc ccctataacg gaatagagaa tcaatacttt ttccggatta
    22141 aaacgcgtca taaggatgtt ggctataaac ttgccaataa agaagcaggc aaagctatag
    22201 accatgaagt ttgaagcatc acgttcgttg atatcgccca actccagcgc cagacggatg
    22261 gtaaatgacc atactgcgac ctgcataccc acataaagga actgcgccac aataccgcga
    22321 cgaaagcgcg gatttctagc cagatagcgc agcgtatcca ttgctgacgg gcgtttatag
    22381 tgacttgtct gtgccacttt acaggttggg aagcgggtta aaaggaacaa caccatgacc
    22441 acaaccagaa tcataatcat atacttatac ggttcaaggg tgttctctaa cat
//
LOCUS       DADMFL010000062        22423 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.062, whole genome shotgun sequence.
ACCESSION   DADMFL010000062 DADMFL010000000
VERSION     DADMFL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.062"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..893)
                     /locus_tag="JNZ27_002398"
     CDS             complement(<1..893)
                     /locus_tag="JNZ27_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY8664701.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNL"
     gene            1391..1831
                     /locus_tag="JNZ27_002399"
     CDS             1391..1831
                     /locus_tag="JNZ27_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8664702.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLSPE"
     gene            1853..1945
                     /locus_tag="JNZ27_002400"
     CDS             1853..1945
                     /locus_tag="JNZ27_002400"
                     /inference="COORDINATES: protein motif:HMM:NF014814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664703.1"
                     /translation="MASGVSRQKVAIIYDVGESTLYKRFPARVI"
     gene            complement(2284..3132)
                     /locus_tag="JNZ27_002401"
     CDS             complement(2284..3132)
                     /locus_tag="JNZ27_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8664704.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3341..3976
                     /gene="yfhb"
                     /locus_tag="JNZ27_002402"
     CDS             3341..3976
                     /gene="yfhb"
                     /locus_tag="JNZ27_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY8664705.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4001..4537
                     /gene="tadA"
                     /locus_tag="JNZ27_002403"
     CDS             4001..4537
                     /gene="tadA"
                     /locus_tag="JNZ27_002403"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY8664706.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(4534..6090)
                     /gene="mltF"
                     /locus_tag="JNZ27_002404"
     CDS             complement(4534..6090)
                     /gene="mltF"
                     /locus_tag="JNZ27_002404"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY8664707.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6348..10235
                     /gene="purL"
                     /locus_tag="JNZ27_002405"
                     /gene_synonym="purI"
     CDS             6348..10235
                     /gene="purL"
                     /locus_tag="JNZ27_002405"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY8664708.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISVFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10793..12220
                     /gene="qseE"
                     /locus_tag="JNZ27_002406"
     CDS             10793..12220
                     /gene="qseE"
                     /locus_tag="JNZ27_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY8664709.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12385..13098
                     /gene="qseG"
                     /locus_tag="JNZ27_002407"
     CDS             12385..13098
                     /gene="qseG"
                     /locus_tag="JNZ27_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY8664710.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13088..14422
                     /gene="glrR"
                     /locus_tag="JNZ27_002408"
     CDS             13088..14422
                     /gene="glrR"
                     /locus_tag="JNZ27_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY8664711.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASLRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14483..14821
                     /gene="glnB"
                     /locus_tag="JNZ27_002409"
     CDS             14483..14821
                     /gene="glnB"
                     /locus_tag="JNZ27_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY8664712.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14866..16056)
                     /gene="hmpA"
                     /locus_tag="JNZ27_002410"
     CDS             complement(14866..16056)
                     /gene="hmpA"
                     /locus_tag="JNZ27_002410"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY8664713.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16383..17636
                     /locus_tag="JNZ27_002411"
     CDS             16383..17636
                     /locus_tag="JNZ27_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY8664714.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(17834..19027)
                     /locus_tag="JNZ27_002412"
     CDS             complement(17834..19027)
                     /locus_tag="JNZ27_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY8664715.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            19145..21721
                     /locus_tag="JNZ27_002413"
                     /pseudo
     CDS             19145..21721
                     /locus_tag="JNZ27_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189288.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
BASE COUNT         5358 a         5576 c         5966 g         5523 t
ORIGIN      
        1 tgtaaatttt gcggcaacat ctggcgtaaa aacagtctgt tttggtctga cagggaaata
       61 cgaaactgag gcccgttaaa atttggaacc gaagtttgct gtattaagaa tagcagagtt
      121 tgatctgata aggggttatt tgcaatctca atagttatat gaggatctaa acgaaaaaga
      181 tttgtcggga ttgtagttat ttggttgttt tcaaaataaa aaaatcttct catgataata
      241 ttttcttgga tgtttggtaa gttgcttaat ctattgctat atgcaaaaag aagttgcagt
      301 ctcctaggta aagggggcag ttcagtaaga tggttgtgcg caaccttaag tatcttaaga
      361 ttagaaggca gagatggaag agtttgcagt tcgttgttcc taacctcaag tttttttaaa
      421 ttcaagggta atgaaggcag tgtggataac ctattatact ctgcgcttag tgatttgatg
      481 gtgtctggta ataacggcag attctctaat tgattttcgg acacatccaa agttattaga
      541 taatgtggta atgcagatat atgtgttaag aaattatctc ttgcaataag ttctttaata
      601 ttttcaggta aatcagtaag gatggttaat ttattacagc tcacatcaag ctttttaatg
      661 caagcaggga gtacaggtaa cgaggaaagc gccaaggctc gcaggtccag tttatgcccc
      721 ttagaggtaa gacatgcacg aagtcttctt accgcctcag tacgctgttc ttccggtaac
      781 ccctgtttct cccaatcatc ccattcggac aaataatggt tgggggatgt aattgtgctg
      841 atatgtggaa ataaacagga tgaaatattt ctgagcatat taatttctcg catatggata
      901 tggaattaga ttgtgaataa attgtattta atgtatctct cctaatgagg agggtgtttc
      961 agaatattcg ccataaggga atgagtgtta tatcatgctt tctaccctgg taagtatatt
     1021 tgcttttttc gtatatttgg ttcatctttt tttgatgttt tacatattca caatgcagag
     1081 gatttatacc agaagacatt agtcttgagt aagatactac aacatctcca actattttag
     1141 ccagcctgac tacttgactt ggtaatattt gttgctaata atcatgtata aaataagctt
     1201 atatacatag taacttcagc aaaggattta gtaatgatcc aaatcatatc tcaatgtact
     1261 gtttttttct ttaaaaaaac agtacattct attttataat gactgtgtat tcccatggaa
     1321 ggagatcacc caattctccc cccccccccc tgacttttga cgcagtgtct tgttaacagg
     1381 gggatttacc atgcttattg gctatgtacg cgtatcaaca aatgaccaga ataccgatct
     1441 acaacgtaat gcgttgaact gtgcaggatg cgagctgatt tttgaagaca agataagtgg
     1501 tacaaagtcc gaaagaccgg ggctgaaaaa actgctcagg acattatcgg caggcgacac
     1561 actggttgtc tggaagctgg atcggctggg gcgcagtatg cggcatcttg tcgtgctggt
     1621 ggaggagttg cgcgaacgag gcatcaactt tcgtagtctg actgattcaa ttgataccag
     1681 cacaccaatg ggacgctttt tcttccatgt gatgggtgcc ctggctgaaa tggaacgtga
     1741 actgattgtt gaacgaacaa aagcaggact ggaagctgct cgcgcacagg gacgaattgg
     1801 cggtcgccgt cccaaacttt caccagaata atgggcgcag gccgggcgac taattgcatc
     1861 aggagtttct cgccagaagg tggcgatcat ctatgatgtt ggcgaatcga ctttgtataa
     1921 gaggtttcca gccagggtta tataaaaccg cagacacggc gtatacaagg acgtgctacg
     1981 gttgactgaa aagttaatgt tgtatgtcac aggccttttg cgccgtaaag ccccgtcctt
     2041 tagggcgcgg aggacgttaa agaaagtggt gttctttgtg ttgctgaact tatgcaaaat
     2101 ttttttgcac cataaatcca acaacacatt caataacaca ttgctgtatc actatgaaaa
     2161 taaatgtgtt tttatttgat ttcacacata tcacaattga tgcatttttt agtgattaac
     2221 agcgccataa ggaaactctc aaaacaacac aaaccgggcg ggcattatac gcgcattctt
     2281 tactcagacc agttttttca ccagcgcttc actatgacga atacgttctg gtgccagttc
     2341 cagatcctgc tgaatcagcg caatgaacag caaatccgtt aacattccct gagcgtgaca
     2401 agcagagatt gaagcactgt ttgtcgcttg ttcttcggca atggtataca ggcaatgaga
     2461 agcacgctgt tgcagggcat tcggagtaaa gccggtaatc gccagcactt ttccgcccac
     2521 tcgcagcatc tcatctgccg ccaggtttaa ctcgcgtcgt acaccggtgt aggaaatggc
     2581 taataacaga tcgtcagggg acgacgcctg tactgttgcg agtagcgcat gcatatcgcg
     2641 cagtgcggca gcattgaagc caatcttcat cagcttccag gcaaagtttt gcgccaccag
     2701 acccgaagcg ccaataccgg tcagaattat ccgccgcgca gagcgcaaca ttgttacgca
     2761 ttcatgcagt ttctcttcac tattaacgtt tagcgttgcg tacatagcgg cagtattttc
     2821 tttaatcagt ttttcgccga ccagccgtaa cggatcatca ccgcggattt ggttatgaat
     2881 gggcacggag ggcgattccg gctggcttgc cagcgcttca ctcaacgcca acttaagcgc
     2941 cggaaaacct ttatagccga gtttttgcgc gaacttcacg acgctggact gactgactcc
     3001 ggcttcgttg gccagttgct gagagcttaa atggcgcgcc gtatcaggtt gtagcagcag
     3061 ataatccgcc agttttttat cactctgggc aagcccctgg taacgctgac ggatacgaag
     3121 taagccgttc atatctttcc tcgcgatgaa atagtggcga caacaggcaa tatgagacac
     3181 acgcgagtga attttatatt ccattgccgc taattgccat gaattaaaaa ttccagttag
     3241 ccatgatgcg gtcgattgtt gccggaaaag tgatgatcta tctgttaggc tatgtagtag
     3301 cgaaaattaa tcaggatgtt tcagtccaga ggagtatggt ttggcaactc acgagcgtcg
     3361 tgtggtgttt tttgacttag atggaacatt gcatcagcag gatatgttcg gcagttttct
     3421 gcgctattta ctgcgtcgcc aaccgctgaa tgcgttactt gtcctgccgt tgttaccgat
     3481 tatagccatt gcgttattga taaaaggtcg tgcggcacgc tggccgatga gtctgcttct
     3541 gtgggggtgc acttttggtc acagcgaagc acgtttacag acgttgcagg ccgatttcgt
     3601 gcgctggttt cgcgacaatg ttaccgcctt tccgctggtt caggagcgat taaccaccta
     3661 cctgttaagt tccgatgctg atatctggtt gattaccggc tctccgcagc cgctggttga
     3721 agcggtttat ttcgatacgc cctggctgcc gcgggttaat cttatcgcca gccaaattca
     3781 gcgtggctat ggtggttggg tattgacgat gcgttgtctg ggacatgaaa aggtcgcaca
     3841 actggagcgc aaaatcggca cgccgctgcg gctgtacagt ggctatagcg acagtaatca
     3901 ggacaatccg ctgctttatt tctgtcagca tcgttggcga gtaaccccgc gcggtgaact
     3961 ccagcaactg gaatagagta aagcatagcg tccgtgtata atgcgccgcg cttttataac
     4021 cggagttttc tttttgtctg aagtcgaatt tagccacgaa tactggatgc gtcacgcgct
     4081 gacgctggcg aaacgtgcct gggatgagcg ggaagtgccg gtcggcgcgg tattagtgca
     4141 taacaatcgg gtaatcggcg aaggctggaa ccgcccgatt ggtcgccatg atcccaccgc
     4201 acatgcagaa atcatggccc tgcggcaggg tggtctggtg atgcaaaatt atcgtctgat
     4261 cgacgccacg ttgtatgtca cgcttgaacc atgtgtaatg tgtgccggag cgatgatcca
     4321 cagtcgcatt ggtcgcgtgg tctttggtgc gcgtgacgcg aaaactggcg ctgcgggatc
     4381 tttaatggat gtgctgcatc atccgggtat gaatcaccga gtggaaatta cggaaggaat
     4441 actggcggat gagtgcgcgg cgttgctcag tgacttcttt cgcatgcgcc gccaggaaat
     4501 taaagcgcag aaaaaagcgc aatcctcgac ggattaattt tgtttctctt cactcccttt
     4561 cctggaaaac agcagagagg gagaatgggt caaatagttt gatgacgact gggaaagaaa
     4621 cgagagaaaa ggaaatttct ctttgcccaa ctccgtaggc gataccgccg gataatcctg
     4681 cgccagttgc atcgccgctt ctgtagcctg cttctctttc tcttgcagat aacccaccag
     4741 gctaatctga tacttacgaa tattttcgac ataagcgtag gcttcatgcc cacgagcgta
     4801 gccgtaagtc agcttgctgt aatagggttt ctggctaagt aaaggcagac gctgttttac
     4861 gtcagcccaa ctgtcaggat tccctttggt ttttgccgtc agggcgcggg catccagcat
     4921 atgcgcatag cccatattgt acgcagcgag ggcaaaccag atccgttcgt tctccggcac
     4981 actttccggc actttactca tcatatcctg caaataacgc acgccaccgc tgatgctctg
     5041 ttcggcatcg gtacgatccg taatgccgag gctttgcgcg gtatttttgg ttaacatcat
     5101 catgccgcgc acacccgtcg gtgaagtggc ctgtgcatcc cagtgcgatt cctgataagc
     5161 aatagcggcc agcaaacgcc agtcaatttc ttcggcgtat ttctcaaaca ggggctttaa
     5221 ctgcggcagt accgcatcga cggcgcgtaa aaatgtgcgc gtatcgacgt aatcaaaatc
     5281 atcgccatgc cccaggtatt tctcttcaat gcgtgccagc gtaccgtctt cattcatttc
     5341 gttgaagaag tcgagcaggg cggcggaaag ggtattatcg ccatctaacg ggctaaacca
     5401 agtcaccggt tgttcatcgg tgatatcgag cgctacggcg agctccgggt gaacgcgctg
     5461 aaacaggctg atggcgacag aatcagcaat ggtgtaatcg agttttcctt cgatgacatc
     5521 ttccattaat tccgcagagc cttttttgtc gtctaccttc cagcttaatt ccgggaattt
     5581 tgtttctttc agggtctgga gatcgttaac caccacatga cccggtgcaa cggtgagttg
     5641 ctccgccgtc aggttgccca gcgtacgtgg gcgatactga cccactttat aaaccagttg
     5701 ttgtgacacg gaataatagg tagggccagg ctgataattt tttacccgct cactgttata
     5761 gaccagtcct gccgccagca ggtcggcgtt accattatca aggtcgtcaa acagctggct
     5821 gatattctgc cgcacggtca ctttcagttt tacgccgagg taatcggcaa actgtttcgc
     5881 cagttcgtaa tccaggccaa aaggtttccc gttgatttcg ttataagtca ggggagtatg
     5941 aatggtgctc acacgcaact ctccccgcgc ttgaatggcg gcgatacggt tgtcggcttt
     6001 accaaaccag ggaatggatg gccagagagc gaccgcgagc agcagtgcca gaatgccgat
     6061 gaacagataa ttaatcttta attttttcaa ttagttaatt ctctgtgtcg tgcgcgtccc
     6121 agcttgaaaa aacgtaataa tagtgaaagg tttactcata aatgagcggc attttgcgta
     6181 aacctgcgcc agatggcaac ttattacagc cattggcggc acgcgttgct aattcacgat
     6241 ggtgatttta tttccacgca aacggtttcg tcagcgcatc agattcttta taatgacgcc
     6301 cgtttccccc ccttgggtac accgaaagct tagaagacga gagacttatg atggaaattc
     6361 tgcgtggttc gcctgcactg tcggcattcc gaatcaacaa actgctggca cgttttcagg
     6421 ctgccaggct cccggttcac aatatttacg ccgagtatgt ccattttgct gacctcaatg
     6481 cgccgttaaa cgatgatgag cacgcacaac ttgaacgcct gctgaaatat ggtccggcac
     6541 tcgccagcca cgccccgcaa ggcaaactcc tgctggtgac cccgcgtcct ggcaccatct
     6601 ctccctggtc ttcgaaagcg accgatattg cccataactg cgggctacaa caggtaaacc
     6661 gccttgagcg cggcgttgct tactatatag aagccggtac gctgaccaat gaacaatggc
     6721 agcaggttac cgctgaactg cacgaccgca tgatggaaac ggtctttttt gatttagatg
     6781 atgcagagca gttgtttgcc caccatcaac cgactccggt cacaagcgtt gatttgctgg
     6841 ggcagggccg tcaggcgctg atcgacgcta acctgcgtct tggcctggct ctggcggaag
     6901 atgaaattga ctatctgcag gatgctttca caaagcttgg tcgtaacccg aacgacatcg
     6961 agctgtatat gttcgctcag gcgaactctg agcactgccg ccacaaaatt tttaacgccg
     7021 actggattat cgacggtgaa caacagccga aatcgctgtt caggatgatc aaaaatactt
     7081 tcgaaaccac gccagatcac gttctctctg cttataaaga taacgccgcc gtaatggaag
     7141 gttctgaagt gggccgctac tttgctgacc acgaaacggg ccgctacaat ttccatcagg
     7201 aaccggcgca catcctgatg aaagtcgaaa ctcacaacca cccgacggcg atttctccgt
     7261 ggccgggcgc ggcgaccggt tccggcggtg aaatccgcga tgaaggtgcc accgggcgcg
     7321 gcgcgaagcc gaaagctggt ttggttggtt tctccgtatc caacctgcga attcctggct
     7381 tcgaacagcc gtgggaagaa gatttcggta agcctgagcg cattgttacc gcgctggaca
     7441 tcatgaccga aggcccgctg ggcggtgcgg cgtttaacaa cgaatttggt cgtccggcac
     7501 tgaacggcta cttccgtact tatgaagaaa aagtgaacag ccacaatggc gaagagctgc
     7561 gcggttatca caaaccgatc atgctggcgg gcgggatcgg caacattcgc gccgatcacg
     7621 tacaaaaagg cgagatcaac gtcggtgcga agctggtcgt tctcggcggc ccggcaatga
     7681 acatcggtct tggcggcggc gcggcgtctt ccatggcgtc tggtcagtct gatgccgacc
     7741 tcgactttgc ttccgtacag cgcgacaacc cggagatgga gcgtcgttgc caggaagtga
     7801 tcgaccgttg ctggcagctt ggtgatgcta acccaattct gtttatccac gacgtcggtg
     7861 cgggcggtct ttctaacgcg atgccggagc tggtgagcga cggcggacgc ggcggtaaat
     7921 ttgaactgcg cgatattcta agcgacgaac cgggcatgag cccgctggaa atctggtgta
     7981 acgaatccca ggaacgctac gtgctggcgg ttgctgccga tcaattaccg ctgtttgacg
     8041 aactgtgtaa gcgtgagcgc gcaccctacg cggtgattgg tgaagcgacc gaagaactgc
     8101 atctttctct gcacgatcgt cattttgata atcagccgat cgatctgccg ctggacgtcc
     8161 tgcttggtaa aacgccgaag atgacccgcg atgtacaaac gctgaaagcg aagggcgatg
     8221 cgctggtccg tgaagggatc accattgcag acgcggtgaa acgcgtactg catctgccga
     8281 ccgtggcgga gaaaactttc ctggtgacca tcggtgaccg cagcgtaacc ggcatggtgg
     8341 cgcgcgatca gatggtgggg ccgtggcagg tgccggtcgc taactgcgcg gtcactaccg
     8401 ccagcctcga cagctactac ggtgaagcga tggcgattgg cgagcgtgcg ccggttgcgc
     8461 tgctggattt cgccgcctct gcccgtctgg cggtcggtga agcgttaacc aatattgccg
     8521 caacacagat tggcgatatc aaacgcatta aactttccgc caactggatg gcggcggcag
     8581 gccaccctgg tgaagatgcg ggcctgtatg aagccgttaa agccgtgggt gaagaacttt
     8641 gtccggcgct gggcctgacg atcccggtgg gtaaagactc tatgtcgatg aaaacccgct
     8701 ggcaggaagg taacgaagag cgcgaaatga cgtcgccgct gtcgctggtg atttctgtat
     8761 ttgcccgcgt ggaagatgta cgtcacacca tcacgccgca gctttgtacc gaagataacg
     8821 cactgctgct gattgatttg ggcaaaggca ataacgcgct gggcgcaacg gcgctggcgc
     8881 aggtttatcg tcagcttggc gacaaaccgg cagatgtacg cgatgttgtc caactgaaag
     8941 gcttctatga cgcgattcag gcgctggttg cacagcgtaa gcttctggcg tatcacgacc
     9001 gctctgatgg cggcctgctg gtaacgctgg cggaaatggc ctttgcaggt cattgtggca
     9061 ttgacgcgga tatcgcctct ctgggtgacg atcgcctggc ggcgttgttt aacgaagaac
     9121 tgggtgcggt gattcaggtt cgtgccgctg accgtgaagc ggtcgagtcc gtactggcac
     9181 agcatgggct tgcggattgc gtccattatg ttgggcaggc ggtttccggt gaccgttttg
     9241 tgattaccgc caacgggcag actgtattca gcgaaagccg cactacgttg cgtgtctggt
     9301 gggcagaaac tacctggcag atgcagcgcc tgcgtgacaa cccggagtgt gccgatcagg
     9361 agcatcaggc gaaatctaac gacgccgatc cgggcctgaa tgtaaaactg tcgttcgata
     9421 tcaacgaaga tgtggcagca ccgtatattg ccactggcgc acgtccgaaa gttgccgtac
     9481 tgcgtgagca gggcgtgaac tcgcatgttg aaatggcggc agctttccaa cgtgggggct
     9541 ttgatgctat cgacgtgcat atgagcgacc tgctgactgg acgtacggga ctggaggatt
     9601 ttcacgccct ggtcgcgtgc ggaggtttct cctacggtga tgtgctgggt gccggtgaag
     9661 gttgggcgaa gtctatcctg ttcaatgacc gtgtacgcga tgagtttgca accttcttcc
     9721 atcgtccgca aacgctggcc ctgggggtat gtaacggttg ccagatgatg tctaacctgc
     9781 gtgaactgat cccggggagc gagttgtggc cacgttttgt gcgtaacacc tccgatcgct
     9841 ttgaagcgcg tttcagcctg gttgaagtaa cccaaagccc gtctctgctg ttgcagggga
     9901 tggtgggctc gcaaatgccg attgctgtct ctcatggtga agggcgcgtg gaagtgcgtg
     9961 atgcggcgca tctggcggca ctggaaagca aagggctggt ggcactgcgc tatgtcgata
    10021 acttcggcaa agtcactgaa acctacccgg ctaacccgaa cggttcaccg aacggtatta
    10081 cggcggttac gactgaaagt ggtcgagtca ccattatgat gccgcacccg gaacgtgttt
    10141 tccgtactgt cagcaactcc tggcatccgg aaaactgggg cgaggatggc ccatggatgc
    10201 gcattttccg caatgcgcgt aagcagttgg ggtaagtcat cagttcattg gttttgcagc
    10261 ccggatgcgg cattaagcac caaccactat cataagctcc caactccggg agcttttttg
    10321 tgtctgtaaa tcacgacaat gggtggttta ccgtgtcgct ttctggcgac acttaaatga
    10381 ttgattttat tgtaatctaa taagtttatc ttaagttgtc tctttttagc gacacagtgg
    10441 ctgatttaca ttcaaatagc gaagaagcca taaatctctc aaagcaacgt ataatcagta
    10501 cgttaccaaa ctattttctt tattggcaca gttactgcat aatagtaagc agtggctcat
    10561 tcaccgactt atgtcagccc cttcgggacg tgctacataa aattcgaatg acgcacaaca
    10621 aggtgcctgc cgtccaactt ctgatatcag cgtagctata tcaaccatcg ggcgaaacgt
    10681 cgagttaggc accgccttat tccataacaa agccgggtaa ttcccggctt tgttgtatct
    10741 gaacttcccc tcggttagca tcaggctatt cgcgtctgac gagagtaaca ccttgaaacg
    10801 ctggcccgtt tttccccgct cattacgaca actggtaatg ctggcatttt tgctaattct
    10861 actacccctg ttggtgctgg catggcaagc ctggcaaagc ctgaatgcgc ttagcgatca
    10921 ggcggcgctg gttaaccgca ctacgcttat tgatgcccgg cgtagtgaag cgatgaccaa
    10981 cgcggcgctg gagatggagc gtagctaccg tcagtattgc gtgctggacg accctacgct
    11041 tgcgaaggtt tatcaaagcc agcgcaagcg ttacagcgaa atgctcgatg cccacgcagg
    11101 cgtactgcca gacgataaac tttaccaggc attgcgtcag gatttgaaca atctggccca
    11161 acttcagtgt aacaacagtg gtcccgatgc tgccgccgcc gcgcgtcttg aagcctttgc
    11221 cagtgccaat accgaaatgg tgcaggccac gcgcacagtg gtgttctctc gtgggcagca
    11281 acttcagcgt gaaatcgccg aacgtgggca atattttggt tggcaatcgc tggtgctatt
    11341 tctggtgagt ctggtgatgg tgctactttt cacgcggatg attatcgggc cggtgaaaaa
    11401 tatcgagcgc atgatcaacc gtctggggga agggcgttct ctgggcaata gcgtttcgtt
    11461 ctgtggaccg agcgagttac gctcggttgg gcaacgtatt ctttggttaa gtgagcgcct
    11521 gtcatggctg gaatcccaac gccatcaatt tttaagacat ttatctcatg aattaaaaac
    11581 gccactggcg agtatgcgcg agggcactga attactggct gatcaggttg tcgggccgct
    11641 tacgccagag caaaaagagg tggtgagcat tcttgatagc agcagctgca atttgcaaaa
    11701 actgatcgaa caactgcttg attacaaccg taaacaggcg gacagtgcgg tggaactgga
    11761 gaatgttgaa ttagcaccgc tggtggagac agtggtttct gctcatagcc tgcccgcacg
    11821 ggctaaaatg atgcataccg acgtcgatct caaagcaaca gcttgcctgg cggagccaat
    11881 gctgctgatg agcgtactgg ataatcttta ctccaatgcg gtgcactacg gggctgaatc
    11941 cggtaacatt tgccttcgca gcagtttaca tggtgcgcgg gtttatattg atgtcatcaa
    12001 tacaggcacg cccattccgc aagaggaacg cgccatgatc ttcgaaccct tttttcaggg
    12061 aagccaccag cgaaaagggg cggtgaaggg cagcggtctg ggattaagca ttgccaggga
    12121 ttgtattcgc cgtatgcaag gggaactgta tctggtcgac gagagcgggc aagacgtttg
    12181 tttccgcatt gaattaccgt cgtcgaaaaa cacgaaataa tgctaacagc ctatcctatc
    12241 aggctatttt atttgtcagt tcggccccgg gtagtgctcg aaatcttcac gtacgatatg
    12301 tacgccccgg tttctccgcg ctgtccatgt ccagactgtc tgcaacaatt acgcccactg
    12361 ggataggctt taagtctggt gaatatgcga cacatttttc aacgattatt gccccgacgg
    12421 ctgtggctgg cagggctgcc atgtctggca ttgctgggtt gcgtgcagaa tcacaataag
    12481 ccagccattg atacgccagc agaagaaaaa attccggttt atcaactggc tgactatctt
    12541 tctactgaat gtagtgacat ctgggcgctg caaggtaaat caaccgaaac caatccgctt
    12601 tactggctgc gggcgatgga ttgtgctgat cgtttaatgc ctgcgcagtc gcgtcagcaa
    12661 gctcgccaat atgacgacgg cagctggcaa aataccttta aacaagggat tttgctcgcc
    12721 gacgccaaaa ttacgccgta tgagcgccgc cagctggtcg cgcgtattga agcgttaagt
    12781 accgagatcc cggcacaggt tcgcccactg tatcaactct ggcgcgacgg tcaggcgctg
    12841 caactgcaac tggcggaaga gcgccaacgt tacagtaaac tccagcagtc cagcgacagc
    12901 gagctggata cattgcgcca gcaacatcac gttctacagc agcaactgga actcaccacc
    12961 cgcaagctgg aaaacctgac cgatattgaa cgccagctct cgacccgcaa accggctgga
    13021 aatttctcgc cggatacgcc gcacgagagt gaaaagcccg cgccatccac ccatgaggtc
    13081 actcctgatg agccataaac ctgcgcattt attattggtc gatgacgatc cgggattgct
    13141 gaaactgctt ggcctgcgcc tgaccagcga aggctacagt gtggtcacgg cggaaagtgg
    13201 cgctgaagga ttacgggtac tgaatcgcga aaaagtagat ttagttatca gcgacctgcg
    13261 gatggatgaa atggacggta tgcagctatt tgctgaaatc cagaaagtgc agccgggaat
    13321 gccagtaatt attcttactg cgcatggttc tatccccgat gccgttgctg caacacagca
    13381 gggcgttttt agtttcctca ccaagcctgt cgataaagat gcgctatatc aggcaattga
    13441 cgatgcgctg gagcaatccg cgccagccac cgatgaacgc tggcgcgagg caattgtcac
    13501 ccgcagcccg ctgatgctgc gtttgctgga acaggcgcgg ctggtggcgc aatcagacgt
    13561 cagcgttttg attaacggtc agagcggcac cgggaaagag attttcgccc aggctatcca
    13621 caacgccagc ctgcgcaaca gcaaaccatt tattgctatt aactgtggcg cattgcccga
    13681 gcaattgctg gagtcggagc tgtttggtca tgcgcgtggc gcgtttactg gcgctgtcag
    13741 caatcgcgaa ggtttattcc aggcggcgga aggcggaacg ctatttctcg atgaaattgg
    13801 cgatatgcct gcgccgttac aggtcaaact gctgcgcgtg ttgcaggagc gtaaagtgcg
    13861 cccgctgggc agtaaccgcg atattgatat caatgtgcgg attatttctg ccactcaccg
    13921 tgatctgtca aaagcgatgg cgcgcgggga attccgcgaa gatctctatt accgcctcaa
    13981 cgttgtcagc ctgaaaattc cggcactggc ggagcgcaca gaagacattc cgctactggc
    14041 aaatcacctg ttgcgccagg cggcagagcg acataaaccg tttgtccgcg cgttctctac
    14101 cgatgcgatg aaacgcctga tgaccgcgag ctggccgggt aatgtgcgcc agttggtcaa
    14161 cgtgattgaa cagtgcgtgg cgctgacctc atctccggtg attagtgatg cgctggtgga
    14221 gcaggcgctg gagggtgaaa atacggcgct gccaaccttt gttgaggcac gtaatcattt
    14281 tgaactcaac tatttgcgta agctgctgca aatcaccaaa ggcaacgtca cccacgcggc
    14341 gagaatggcg gggcgcaacc ggacagaatt ttataaactg ctttcccgac acgagctgga
    14401 tgcaaacgat ttcaaggaat gaattggcgt tatgtgttac gtttagcaga tcaaaagaca
    14461 ggcaaccttt tcaaggaata gcatgaaaaa gattgatgcg attataaaac ccttcaagct
    14521 ggacgatgtc cgcgaagcac tggccgaagt cggtattacc ggcatgacgg tgaccgaagt
    14581 gaaaggcttt ggtcgccaga aaggccatac cgagctgtac cgcggcgcgg agtatatggt
    14641 ggattttctg ccgaaagtga aaattgagat tgtcgtaccg gacgacattg tcgatacctg
    14701 tgtcgatacc attattcgca cggcgcaaac cggcaaaatc ggtgacggta aaatcttcgt
    14761 ctttgacgtg gctcgggtca ttcgcatccg taccggtgaa gaggacgacg cggcaattta
    14821 aaccgcgtca agggttgccg gatgtttcca tccggcaaca tcaaattaca gcaccttatg
    14881 cgggccaaag cattcgtaat gaatgttttc ctgcttcacg cccagatcca ctaactgttt
    14941 cgcggtaaac tgcatgaagc caaccgggcc gcagagatag aactgcattg tcggatcgct
    15001 gaacgcacct tccagtttgc tcaaatccat cagaccttcg ctatcaaact gacctttagc
    15061 gcgatcggct tcgctcggct gacgatacca ggtgtgcgcg gtaaagcgcg gcagtgactg
    15121 ccccagttcc ttaacttcat cagcaaaggc gtgaacttcg ccattttctg ccgcatggaa
    15181 ccagttcact tgtgctgtgt ggcctgcttt tgccagcgtg tcgagcattg ccagcattgg
    15241 cgtttgacca acaccggtag agattaacgt cactggtgtg tcatctgcga cagccataaa
    15301 gaaatcacct gctggagcga ccagtttcac gacatcgcca acattggcgt gattgtgcaa
    15361 ccagttggat acctgcccac cctcttcgcg tttcaccgcg atacgatagc ctttgccatc
    15421 cggtttgcga gtcaaagagt actgacgaat ttcctgatgc gggaaacctt ccggcttcag
    15481 ccagacgccg agatattgcc ccggacggta ttctgccact gcgccaccgt cgaccggctc
    15541 cagttcgaag ctggtgataa gcgcgctgcg cggtgtttta gccacaatgc ggaaatcgcg
    15601 agtaccttcc caaccaccgg ctttgctggc gttttcgtta tagatttccg cctcgcgatt
    15661 gataaataca ttagccagta caccataggc tttaccccac gcgtccagca cttcctgccc
    15721 cgggctgaac atttcgtcca gcgttgccaa caggtgttca ccgacgatgt tgtactgttc
    15781 cggtttgatc tggaagctgg tgtgcttctg cgcgattttt tctaccgctg gcagcagcgc
    15841 aggcaggttt tcaatattac tggcgtaggc ggcaatagcg ttaaacaggg cttcacgttg
    15901 atcgccatta cgctggttac tcatgttaaa aatttctttg agttctgggt tatgagtaaa
    15961 catacggtcg tagaaatggg cggttaactt tggccccgtt tccaccagta aagggatggt
    16021 ggcttttact gtagcgatgg tttgagcgtc aagcatatgg tcttcctttt ttgcatctta
    16081 attgatgtat ctcaaatgca tcttataaaa aatagccctg caatgtaaat ggttctttgg
    16141 tgtttttcag aaagaatgtg atgaagtgaa aaatttgcat cacaaacctg aaaagaaatc
    16201 cgtttccggt tgcaagctct ttattctcca aagccttgcg tagccttaag gtaatcgttt
    16261 gcgtaaattc ctttgtcaag acctgttatc gcacaatgat tcggttatac tgttcgccgt
    16321 tgtccaacag gaccgcctat aaaggccaaa aattttattg ttagctgagt caggagatgc
    16381 ggatgttaaa gcgtgaaatg aacattgccg attatgatgc cgaactgtgg caggctatgg
    16441 agcaggaaaa agtacgtcag gaagagcaca tcgaactgat cgcctccgaa aactacacca
    16501 gcccgcgcgt aatgcaggcg cagggttctc agctgaccaa caaatatgct gaaggttatc
    16561 cgggcaaacg ctactacggc ggttgcgagt atgttgatat cgttgaacaa ctggcgatcg
    16621 atcgtgcgaa agaactgttc ggcgctgact acgctaacgt ccagccgcac tccggctccc
    16681 aggctaactt tgcggtctac accgcgctgc tggaaccagg tgataccgtt ctggggatga
    16741 acctggcgca tggcggtcac ctgactcacg gttctccggt taacttctcc ggtaaactgt
    16801 acaacatcgt tccttacggt atcgatgcta ccggtcatat cgattacgcc gatctggaaa
    16861 aacaagccaa agaacacaag ccgaaaatga ttatcggtgg tttctctgca tattccggcg
    16921 tggttgactg ggcgaaaatg cgtgaaatcg ctgacagcat cggtgcttat ctgttcgttg
    16981 atatggcgca cgttgcgggc ctggttgctg ctggcgtcta cccgaacccg gttcctcatg
    17041 ctcacgttgt tactaccacc actcacaaaa ccctggcggg tccgcgcggc ggcctgatcc
    17101 tggcgaaagg tggtagcgaa gagctgtaca aaaaactgaa ctctgccgtt ttccctggtg
    17161 gtcagggcgg tccgttgatg cacgtaatcg ccggtaaagc ggttgctctg aaagaagcga
    17221 tggagcctga gttcaaaact taccagcagc aggtcgcgaa aaacgctaaa gcgatggtag
    17281 aagtgttcct cgagcgcggc tacaaagtgg tttccggcgg cactgataac cacctgttcc
    17341 tggttgatct ggttgataaa aacctgaccg gtaaagaagc agacgccgct ctgggccgtg
    17401 ctaacatcac cgtcaacaaa aacagcgtac cgaacgatcc gaagagcccg tttgtgacct
    17461 ccggtattcg tgtaggtact ccggcgatta cccgtcgcgg ctttaaagaa gctgaagcga
    17521 aagaactggc tggctggatg tgtgacgtgc tggacagcat caatgatgaa gccgttatcg
    17581 agcgcatcaa aggtaaagtt ctcgacatct gcgcacgtta cccggtttac gcataagcga
    17641 aacggtgatt tgctgacaat gtgctcgttg ttcatgttgg atgcggcatg aacaccttct
    17701 caggcctaca aaatcttgta aattcaatat attgcaatct ccgtgtaggc ctgataagcg
    17761 tagcgcatca ggcaattttt cgtttatgat catcaaggct tccttcggga agcctttcta
    17821 cgttatcgcg ccatcaaatc tgtcgtaact gcgcctcaac atacaaatag ccaattccca
    17881 gcacctgttg tgcgcggctt aattgcccga agccaatttg cgtcgctttc acgctcggtc
    17941 cttcgtcgcg gtcgaacggg ttaaatcctg tctggcgaat aatagtatta agccagttct
    18001 caccaaaggc acaactgcga ccgtacaacc aaatctgatt aatattgagg atgttgagga
    18061 agttatacag actcaaacca atggcattgg cagagcgatc aacccagctg gtgatccacg
    18121 gttctccact ctgccaggca gcgattaact gcgcggttgt cagtttttca ggatcaagct
    18181 gagtattaac cggttgtgat tttagccata cccgcgcctg tttttttaat gcacttaacg
    18241 aggcgacggt ttccaggcag ccgtaacgtc cacagtcgca gacgacgcca tccggattaa
    18301 cgatggtgtg accaatctgt ccgctaccat acaaactgcc gcgataaatt tgctcgttaa
    18361 tcacgaacga cgagccaatg ccgtaatcaa cgttgatcac gcagaaatcc cgttcctgcg
    18421 aattattttg ccatttctcc gcctgcgcca gcatcacgca gtcattatcg accagcaccc
    18481 gaatgcccag cttctcttcc agcagatact tcacctcaac tggcgttgtc cacggcgctt
    18541 gcggcatggt ttgcgatacg ccggtcaccg gatcaacctg cccgtggatt gccagcgcca
    18601 gattgatggt gcggtccggc cacaatttac ggtggcgatg ccagcatttt tcgatttcgg
    18661 acagcagcgc ctgcggcgtc ggtacatcaa tctgtaaata ctcaaattcg cctttcggac
    18721 ttaaacaagc attagcaacc tgacactcaa tactggtggg cgtcacgttc aggcacagcg
    18781 tccagtcacc ttccggcgca atcagccatg taccgctact atgcccgcgc gtctggcttt
    18841 catcgtcaat attcaccacc cgtttttcgc tttccagttc ctgcaaaata ttactgactg
    18901 ccggaatcga aatttgcgcc agccgggcca gcgttgattt attggcgcgc ttttgccggt
    18961 acagcagttc cagaatcacg catttgttat ggtggcgaat ctgttgattg ttaatgcagg
    19021 ctctcatctt cactaaacct tgtttagtta attgcgtgat tattccgcga aaacattgcc
    19081 agattgttca atacactgcc acaaatcttt taattcagta tgtcttgtta atattgaggg
    19141 caccatgact ccagtaaaag tgtggcaaga gcgcgttgag atcccgacct atgaaaccgg
    19201 gccgcaggat atacatccca tgttcctgga aaatcgcgtt tatcagggat cgtccggcgc
    19261 ggtttatccc tacggcgtga ccgatacgct gagcgagcag aaaaccctga aatcctagca
    19321 ggcggtgtgg ctggaaaacg actacatcaa agtgatgatc ctgccggaac tgggcggtcg
    19381 ggtgcatcgc gcatgggata aagtgaaaca gcgcgatttt gtctatcaca atgaagtcat
    19441 taaacctgcg ctggtggggc tgctgggacc gtggatctcc ggcgggattg agtttaactg
    19501 gccgcaacac catcgcccga ccacctttat gcccgttgat ttcaccctcg aagcccatga
    19561 agacggtgca cagacggtgt gggtcggcga aacggagccg atgcatggtt tacaggtgat
    19621 gacaggtttc accctgcgcc ctgaccgggc ggcactggaa atcgccagcc gcgtctataa
    19681 cggcaacgcc acgccgcgtc atttcttgtg gtgggccaac ccggcagtga aagggggtga
    19741 agggcatcag agcgttttcc caccggatgt gactgcggtg tttgatcacg gcaaacgggc
    19801 cgtctccgct ttccccatcg ccaccggcac ttactacaaa gtggactact ccgccggagt
    19861 ggacatttct cgctataaaa atgtgcctgt tccaacctca tatatggccg aaaaatcgca
    19921 gtacgatttt gtcggcgcgt ggtgtcacga tgaagatggc ggtttgctgc acgttgccaa
    19981 ccaccatatt gcgccaggta aaaaacagtg gagttgggga cacagtgaat ttggccaggc
    20041 gtgggataag agtctgaccg acaataacgg cccgtatatc gaactgatga ccggtatttt
    20101 tgccgataac cagcctgatt ttacctggct tgatgcttac gaagagaagc gtttcgagca
    20161 gtatttcctg ccttatcatt ctctgtgcat ggtgcaaaat gcctcccgcg atgcggtgat
    20221 aaaactccag cgtagtgagc gggggattga gtgggggctg tatgccatct ctccgttgaa
    20281 cggataccgt ctggcgatcc gcgaaatcgg caaatgcaac gcgttgctcg atgatgccgt
    20341 ggccctgaca ccagcgaccg ccatccaggg cgtgttgcac ggtatcaatc ctgacagact
    20401 gaccattgaa ctctccgatg ccgacggcaa tattgtactg agttatcagg aacatcagcc
    20461 gcaggagttg ccgttgccgg acgtcgccaa agcgccactg gcagcacaag acattaccag
    20521 tacagatgaa gcctggttta tcggtcagca tctggagcaa tatcatcacg ccagccgttc
    20581 accgttcgat tactacctgc gcggcgtggc gctggacccg ctggattatc gctgtaacct
    20641 ggcgctggcg ctggcgatgc tggaatataa ccgcgcagat ttcccgcaag cggtggcgta
    20701 tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa aacccgcagt gtggacaagc
    20761 aagtttgatt cgcgccagtg cttacgaacg tcagggacaa tatcaacaag ccgaagagga
    20821 tttctggcgt gcggtctgga gcggcaatag taaagcaggc ggctattatg gtctggcacg
    20881 actggcggcg cgtaatgata acttcgacgc gggtctggat ttttgccaac aaagtcttcg
    20941 tgcctgccca accaatcagg aagtgctttg cttgcataat ctgctgctgg tgttaagtgg
    21001 tcgtcaggac aacgcgcgtt tgcagcgcga gaaactgctg cgtgattatc cgctgaacgc
    21061 cactctgtgg tggctgaact ggttcgatgg tcgtagcgaa tcagccctcg cgcagtggcg
    21121 cggtctgtgt cagggacgcg acgttaacgc tctgatgacc gccgggcaac tgattaactg
    21181 gggaatgccc accctggcgg cagagatgct gaacgcactg gactgccagc gcacgctgcc
    21241 gctttacctg caagccagct tgctgccgaa agccgaacgt ggcgaactgg tcgcaaaagc
    21301 cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg ctggaagaag tggcggcgct
    21361 ggagagtatt gaagagtgct ggtttgctcg ccatttactg gcctgcttct actacaacaa
    21421 acgtagctac aacaaagcca ttgccttatg gcaacgttgc gtagagatgt cgccggagtt
    21481 tgccgacggc tggcgcgggt tagcgatcca tgcgtggaat aagaaacacg attatgagct
    21541 ggccgcgcgt tatcttgata atgcttatca gcttgcgccg caggatgcac gtctgctttt
    21601 cgaacgggat ttgctggata agttaagtgg agccacaccg gagaaacgac tggcgcgtct
    21661 ggaaaataat ctggaaattg cgctgaaacg cgacgacatg accgcagaac tgctcaattt
    21721 gtggcatctc acgggtcagg cagacaaagc ggcggacatt ctcgccacgc gtaaattcca
    21781 cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt atcctcaacc agttattacg
    21841 cgcctggcag catcttgatg ccagagagcc gcagcaggcc agcgaactgc ttcatgccgc
    21901 gctgcattat ccggagaatt taagcgaagg ccgtttaccg gggcaaactg ataacgacat
    21961 ctggttctgg caggcgatat gcgccaacgc gcagggcgat gaaactgaag cgatgcgttg
    22021 tttacgtctg gcggcgaccg gcgatcgcac cattaacatc cacagttatt acaacgatca
    22081 gccggttgat tatctcttct ggcaaggaat ggcgctgcga ctgctgggtg aacagcaaac
    22141 cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa gagatggcga aaaccagtat
    22201 tgaggcggat ttctttgctg tttcacaacc tgacctgtta tcgctgtatg gcgatttaca
    22261 acagcagcat aaagaaaaat gcctgatggt ggcgatgctg gcgtccgcgg gactcgggga
    22321 ggttgcgcaa tatgagtctg ctcgcgctga attgacggcg attaatccgg cctggccgaa
    22381 agcggcatta ttcaccactg tgatgccttt tatttttaac tac
//
LOCUS       DADMFL010000063        22314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.063, whole genome shotgun sequence.
ACCESSION   DADMFL010000063 DADMFL010000000
VERSION     DADMFL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.063"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JNZ27_002414"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JNZ27_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY8664716.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JNZ27_002415"
     CDS             831..971
                     /locus_tag="JNZ27_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664717.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JNZ27_002416"
     CDS             985..1857
                     /locus_tag="JNZ27_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY8664718.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNZ27_002417"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNZ27_002417"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY8664719.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNZ27_002418"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNZ27_002418"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY8664720.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5163..5954)
                     /gene="mazG"
                     /locus_tag="JNZ27_002419"
     CDS             complement(5163..5954)
                     /gene="mazG"
                     /locus_tag="JNZ27_002419"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY8664721.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6050..8284)
                     /gene="relA"
                     /locus_tag="JNZ27_002420"
     CDS             complement(6050..8284)
                     /gene="relA"
                     /locus_tag="JNZ27_002420"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY8664722.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8332..9633)
                     /gene="rlmD"
                     /locus_tag="JNZ27_002421"
     CDS             complement(8332..9633)
                     /gene="rlmD"
                     /locus_tag="JNZ27_002421"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY8664723.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9690..12446
                     /gene="barA"
                     /locus_tag="JNZ27_002422"
     CDS             9690..12446
                     /gene="barA"
                     /locus_tag="JNZ27_002422"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY8664724.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12678..14018)
                     /gene="gudD"
                     /locus_tag="JNZ27_002423"
     CDS             complement(12678..14018)
                     /gene="gudD"
                     /locus_tag="JNZ27_002423"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8664725.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14039..15379)
                     /locus_tag="JNZ27_002424"
     CDS             complement(14039..15379)
                     /locus_tag="JNZ27_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8664726.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15381..16733)
                     /locus_tag="JNZ27_002425"
     CDS             complement(15381..16733)
                     /locus_tag="JNZ27_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664727.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17167..17616)
                     /locus_tag="JNZ27_002426"
     CDS             complement(17167..17616)
                     /locus_tag="JNZ27_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY8664728.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17634..18416)
                     /gene="truC"
                     /locus_tag="JNZ27_002427"
     CDS             complement(17634..18416)
                     /gene="truC"
                     /locus_tag="JNZ27_002427"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY8664729.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18416..18745)
                     /locus_tag="JNZ27_002428"
     CDS             complement(18416..18745)
                     /locus_tag="JNZ27_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY8664730.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19367..19912)
                     /gene="syd"
                     /locus_tag="JNZ27_002429"
     CDS             complement(19367..19912)
                     /gene="syd"
                     /locus_tag="JNZ27_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY8664731.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19980..20828
                     /gene="queF"
                     /locus_tag="JNZ27_002430"
     CDS             19980..20828
                     /gene="queF"
                     /locus_tag="JNZ27_002430"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY8664732.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20940..22304
                     /gene="ppnN"
                     /locus_tag="JNZ27_002431"
     CDS             20940..22304
                     /gene="ppnN"
                     /locus_tag="JNZ27_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY8664733.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5510 a         5915 c         5293 g         5596 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca agcgcttgac cgcgtaattc
     5161 ccttagagat caatttcctg ccgttttacc tgttgccaga cttcttccat tgtttcgagg
     5221 tcaacacctg tcatttccag tccacgcgcg gcaacaatac gctccacttc gcgaaaacga
     5281 cgctcgaatt tttcgttcac tttttgcaat gcgatttctg ctttcgtccc taaatggcga
     5341 gccagattaa ccgtggcaaa cagcaggtcc cccatttcct cctccagttt agcctggtcg
     5401 acaacagcct gccgcgcctc gtacatcacc tcgtcgatct cttcgtagac tttatcaacc
     5461 accgggccaa gcgtcgtcca gtcgaagcca acgttggcgc aacgtttctg gattttttgc
     5521 gcccgcatta aagccggtaa actacgagga atatcgtcca gcgccgaatg ctgcgctttc
     5581 tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa gcacttcact actgttttcg
     5641 gcagaactat cagcaaaaac atgcggatgg cgacgctcta atttatcgct aatagcagcg
     5701 caaatatcat taaagtcaaa gcgcccttct tcctgagcca tttgcgcgta aaacaccacc
     5761 tggaatagca gatcgcccag ttcaccgcga agatcgtcaa aatcttcacg ggcgatggcg
     5821 tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa tggtggcaaa tgtctgctct
     5881 ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct gcataatagt gagcaaacgg
     5941 tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct gtcaggtgta ataccttcga
     6001 gtaaaagata tcgctggata cggctgacgc catatccagc tatgtaagac taactcccgt
     6061 acaaccgacg cgcgtcgata acatccggca cctggttgag tttacccagc acgcgcccca
     6121 gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt cgccagttgc tgtttggtgt
     6181 cgctacggct ggcaacgcca agcacgttca ccttctcgtt ggcgagaatg gtcgtgatat
     6241 cacgtaacaa cccactacga tcattagcca ccacgcggac caccagcgaa tatccggcgg
     6301 agtagctctc accccatacc gcgtcaacaa tgcgttctgg cgcatgggag cgcagttccg
     6361 ccagttgttc gcaatcggcg cggtgtactg aaataccgcg tccctgggta atgaagccga
     6421 caatctcatc tccaggaatc ggctggcagc agcgcgcgat gtggtgcatc aggttaccaa
     6481 caccttcaac caccacacga ccgttatctt tactgcggtt ttgcggtgtg tagctttttt
     6541 gctgaagctg tttcagcgcg gcggcgtcct gctcttcggc actcggctta ttaaattgcg
     6601 attgcaggaa gttcaccatc tgattgagac ggatatcccc gccaccaatc gccgccagca
     6661 actcgtcgac atcattgaag ttgtaacgcg gcagcagatg tttttctgct tctttcaggc
     6721 tgatccccag atgttccagc tcgtcgtcaa ggatttgccg cccagccaga atgtttttgt
     6781 cacggtcctg tttacggaac caggcgtgaa ttttcgaacg cccacggctg gttgtgacgt
     6841 aaccgaggtt tgggtttaac cagtcacggc tggggttcgg ctgtttctgg gtgataattt
     6901 caatctggtc gcccatttgc agctggtagg tgaacggcac aatgcgcccg ccaatttttg
     6961 ccccgatgca gcggtgtccg acatcactgt ggatgtggta agcgaagtcc agcggcgttg
     7021 atcccgcagg caaatcaacg acatcacctt tcggcgtaaa gacatacacc cggtcgtcaa
     7081 acacctgact acgtacttcg tcgagcattt cgccggaatc agccatctct tcctgccacg
     7141 caatcagttt acgcagccag gcaatccggt cttcatgtcc cgaacgtgcg ccgccagctg
     7201 ccgcgccctc tttatatttc cagtgcgcag caacacccaa ctccgcatct tcatgcatct
     7261 gtttggtgcg gatttggatc tcaacggttt ttccacccgg ccccagaacc acggtatgaa
     7321 tagactgata accgtttggt ttcgggttag cgacgtaatc gtcaaactca tccggcaggt
     7381 ggcgatagtg agtgtgcact atccccagtg cggcataaca atcctgtaaa cgctcggcga
     7441 caatacgtac cgcacgcaca tcaaacagct catcgaaggc gaggttcttt ttctgcattt
     7501 tgcgccagat gctgtagatg tgtttcggac gaccatacac ttcagcttta acgccttcag
     7561 ctttcatctc agcgcgcaga tggccgacaa actcttcgat atagtgttcg cggtcgagac
     7621 ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt cgggtggaga taacggaagc
     7681 agtaatcttc cagctcccat ttcagttgcc caatcccaag acggttcgcc aacggcgcgt
     7741 agatattggt gcactctttt gccgccagta cacgttcatc ttccggcgca tcttttactt
     7801 cgcgcagatg agcaatacgc tccgccagtt tgatgactac gcagcgaaaa tcatcgacca
     7861 tcgccaataa catccggcga acgttatcga cctgttcgga ggaaacagaa tcagtgtgcg
     7921 tcgctttcag ctggcggatc gccgccatat cacgcacgcc gtgaataagg ttaacaaccg
     7981 acttaccgac gctctcacgc agcacatctt cgctgactac gttggcatca gccagaggga
     8041 acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa tgtcgagagg atctccacca
     8101 tctcaacacc acgccacaat aacagactgg catccggatg cccctgcgtc tgttgcagac
     8161 aatacgccca ggtttcggct aagcactcac acgacttctg gctggtaata cccagacttg
     8221 cgatccattt ttccggatca aattcaccag ccttattgat atgtgcactt cttaccgcaa
     8281 ccatcgtcct ctcctttagg gaccagacct gccgaaatcg gcaaatcgta actatttaac
     8341 gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt gggaacatat ccagcatcgc
     8401 cagtcgcgca atggtatatc cagcttttaa taacgcttcg ctatcccgag ccagcgttgc
     8461 agggttacag gatacataaa ctatacgaat aggttccagt tttataattt gctgcataac
     8521 acctgcggca cctgctcgcg ccgggtccag caacacttta tcgaagccgt ttttcgccca
     8581 cggctgcttt gtgacatctt cttcaagatt ttcgtgataa aacgtcacat tctgtaagcc
     8641 attaagacgc gcattctgct ggcctttttc caccagcgcc ggaacacctt ctacaccgac
     8701 cacactggca gcttgtgtcg ccaatggcag tgtaaagttg cccataccgc agaacagatc
     8761 cagtacgcga tcttcaggtt gcacatccag ccattccaac gcacgcgcta ccattttttg
     8821 gttcacaccc gcattgacct gaataaaatc gcgcgggcta aaagttaagc gcaacccgtt
     8881 tgagtcatac cagggcatct caccagagac ggtttcgagt atctcactat cgggggcgag
     8941 atacagatcc aggccttcag aatgcgaaaa gcgttccagt ttttcgcgat ctgccgaact
     9001 tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg gttgcctgta ccagttcaac
     9061 atgaccaaga tggcgcatag cttgtaagct gcccagacat gccctgactt tgggcagcaa
     9121 tgcttcaagt tggggcgcta aaatggggca ttgtttgacg tcgacaatgt cactggagcc
     9181 cgctttgcga aaccccatct gaagttgctg tgttttcggt aagtagttca gacttaaacg
     9241 cgcgcggcgg cgatagcccc agggaacatc ggcgatcact tcagagacat cgtgtttcat
     9301 taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa tccacgctgg cgtgttgttg
     9361 ctgacagccg ccgcatacgc caaaatgagg acagcgtggc gtttcgcgtt ccgggctatc
     9421 gcttaaccgg cgtacgactt tagcgcgggc atactgtttt ttatcttcag taacagcaac
     9481 ttccgcgttt tcctgcggca ataatccggg gataaataac gttttgccgt tatgtcgcgc
     9541 cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg gttatgatct gacgcgtcgt
     9601 cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag actttctcaa tttaacagtg
     9661 tgaccttaat tgtcccataa cggaactcca tgaccaacta cagcctgcgc gcacgcatga
     9721 tgattctgat cctggcaccg accgtcctta ttggtttatt gctgagtatc tttttcgtcg
     9781 tgcatcgcta taacgacttg cagcgtcaac tggaagatgc cggtgctagc attattgagc
     9841 cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa tcgcgaatct atcggtcagt
     9901 taataagcgt actgcatcgt cgccattccg atattgttcg cgcgatttcg gtttatgatg
     9961 aaaataaccg actctttgtc acctccaatt ttcatcttga tccctcatca atgcagctcg
    10021 gcagcaacgt gccgtttcct cgccagctca ctgtcactcg tgacggcgat attatgatcc
    10081 tccgcacgcc gattatttct gagagttact cccccgacga atcgcccagt agcgatgcca
    10141 aaaatagtca aaatatgttg ggatatattg cgcttgagct ggatcttaaa tcggttcgct
    10201 tgcagcaata taaagagatc tttatttcct gcgtgatgat gctgttttgt atcggtattg
    10261 cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg tccgattcgc aacatggtga
    10321 ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg ggtggaagga tttatgctcg
    10381 gcgagctgga tatgctgaaa aacggtatca actcgatggc aatgtcgctg gctgcttatc
    10441 acgaagagat gcagcacaat atcgaccagg cgacgtccga tctgcgtgaa acgctggagc
    10501 agatggaaat tcagaacgtt gagttggatc tggcgaaaaa gcgcgcccag gaagcggcgc
    10561 gtattaaatc cgagtttctg gcaaatatgt cacacgagct gcgtacacca ctgaatggtg
    10621 ttattggctt tacccgcctg acgctgaaaa cagaattaac accaacgcag cgcgatcacc
    10681 tgaatacgat tgaacgttcg gcaaataatt tgctggcaat tattaatgat gttctcgact
    10741 tctcgaaact ggaagcaggt aagctgattc tggaaagtat tccattccca ctacgcagca
    10801 cgctggatga agtcgttact ctgctggcac attcttctca cgataaaggg ttggaactga
    10861 cgctcaatat taaaagcgac gtgcctgata acgtgatcgg cgacccactg cgattacagc
    10921 aaatcatcac taacctagtg gggaatgcaa ttaaattcac cgagaatggc aacattgata
    10981 ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca gattgaagtg cagattcggg
    11041 ataccggcat tggtattcct gaacgcgatc aatcgcgctt attccaggcc ttccgacagg
    11101 ctgatgccag tatttcccgc cgtcatggtg gcaccgggct ggggctggtg attacacaaa
    11161 aactggttaa tgaaatgggc ggcgatattt cgttccatag ccagccgaat cgcggttcaa
    11221 ctttctggtt ccacattaat ctcgatctga acccgaacat tattatcgaa gggccatcca
    11281 tccagtgcct cgcaggtaag cgcctggcct atgtcgaacc aaactccgca gcagcgcaat
    11341 gcacgctgga tattttaagt gaaacgccgc tggaagtggt ttatagccca acgttctccg
    11401 cgctgcctcc cgcgcattac gacatgatgt tgttaggcat cgcggtgacc ttccgcgagc
    11461 cgctaacaat gcaacatgag cgattagcga aagcggtatc gatgaccgat ttcctgatgc
    11521 tggcacttcc ttgccatgca caagtcaatg ctgaaaaact caagcaagat ggtatcggcg
    11581 cgtgtctgct gaaaccatta acacctacgc gcctgttgcc tgccctgacg gaattttgtc
    11641 atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag taagctggca atgacagtca
    11701 tggcggttga tgacaacccc gctaacctga aacttatcgg cgcattgctg gaagatatgg
    11761 tgcaacatgt ggaactttgc gatagcgggc atcaggcagt tgaacgggcg aaacagatgc
    11821 cgttcgattt gatcttaatg gatattcaaa tgccggacat ggatggcatt cgggcctgcg
    11881 aactcatcca ccaactcccg catcagcgac aaacgccggt tatcgcggta acggcgcatg
    11941 caatggccgg gcaaaaagag aagctgcttg gcgcagggat gagcgattat ctggcgaaac
    12001 cgattgaaga agagcgattg cataatttgt tgttgcgcta caagcctggc agcggtattt
    12061 cgtctcgcgt cgtgacgccc gaagtcaacg aaattgtggt gaacccgaat gcgaccctcg
    12121 actggcaact ggcactacgc caggcagcag gaaaaaccga tttagcgcgc gatatgctgc
    12181 aaatgttact cgatttcctg cctgaagttc gcaacaaagt tgaggaacag ctggttggag
    12241 aaaacccgga aggcctggtt gatttgattc ataaactgca tggcagttgc ggctatagcg
    12301 gcgtaccacg tatgaagaat ctctgccagc tgatcgaaca acagctacgt agcggtacta
    12361 aagaagaaga tttggagccg gagctgctgg aactgttgga cgagatggat aatgtcgcgc
    12421 gcgaagccag caaaattctc gggtaacgga cgtcttgtcg agtagcaatc ggctttttat
    12481 gacgctggtt tcagattggt gacaaagtgc ctgtgtttat gccggatgcg gcgttaacgc
    12541 cttatccggc ctacaaaacc aattaaattc aatgaattaa aaaatgatgt agacctgata
    12601 catagcacaa caggctattt tgcgtttagc atcagtctca aaccggctcc agatagagcc
    12661 ggttttggtt ttctgtctta acgcaccatg cacgggcgct tgttatcgaa cgtccagcca
    12721 ggaatcagat actgcattcc catcgcatcg tcacgcgcgc caagcccgtg tttctgatac
    12781 agctcatggg ctttcatcac ttgatccata tcgatttcta cacccagccc cggtttttct
    12841 ggcacctgta ccagcccgcc tttgatctca aacggttctt tggtcaggcg ctgattacct
    12901 tcctgccaga tccagtgcgt atcaatagca gtaattttac ccggtgcagc ggcggcaaca
    12961 tgggtaaaca tcgccaggga aatatcgaag tggttgtttg agtgtgaacc ccaggtcagg
    13021 ccaaattcat ggcacatttg cgccacacgt accgaacctt gcatagtcca gaaatgcgga
    13081 tccgccagcg ggatatcaac ggattgtaac gagagcgtat ggcccatctg ccgccagtcg
    13141 gtggcgatca tattggttgc agtcggcagg ccagtcgcgc gacggaactc tgccatcact
    13201 tcacgcccgg agaaaccttg ctccgcacca cacggatctt ctgcataagc cagcgaacct
    13261 ttcaggtatt taccaatttt aattgcttcg ttcagcgacc aggcaccgtt aggatcgagc
    13321 gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa tagactcggc ttcttcttcc
    13381 ccggccagta caccgccttt cagtttgaaa tcgttgaagc catatttttc atacgccgct
    13441 tccgccaggc gcaccaccgc atccggcgtc atcgcttctt catgacgcag gcgataccag
    13501 tcgcatgagt catccggctg gctttgatac ggcagaggtg tggctttgcg attaccgacg
    13561 aagaacagat aaccgagcat ttcgacttcg ctacgctgtt gaccatcgcc cagcagcgat
    13621 gccacgttta cccccagatg ctgccccagc agatccagca ttgccgcttc tatcccggta
    13681 actacatgaa tagtggtacg caaatcaaat gtctgcaaac cgcgcccact agcatcacga
    13741 tcggcaaaag tattacgcac cagcgtcaga acgtttttgt attcacccag cgttttacct
    13801 accaccagcg gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc gggaatttcc
    13861 cctacgccag tgtgaccgga attatctttg ataatcacaa tattacgcgt gaagaacggt
    13921 gcgtgtgcac cactcagatt catcagcata ctgtcatgac ccgccaccgg gatgacctgc
    13981 atttcagtaa caacaggcgt cgtaaattga gaactcatat ttatgtcctt tttcagaatc
    14041 aatgacggcc gaaaacggga cgtttacggt caaaggtcca gccggggatc aggtactgca
    14101 tcggacctgc gtcgttacgc gcaccgcaag gcagacgttt ataggcctca tgtgcctttt
    14161 gtacctgttc ccagtccagt tccacgccca gaccgggcgc atcaggaacg gcaatttttc
    14221 cgtttttaat ctccagcgga tttttggtca ggcgacaatc gccctcctgc caaatccagt
    14281 gggtatcgat agcggtagga ttacccggtg ccgccgcgcc cacatgggta aatatcgcca
    14341 gagagatatc gaaatggttg ttggaatggc agccccaggt cagcccccag tcgtcgcaaa
    14401 gctgcgccac gcggactgca ccggaaagcg tccagaagtg cggatcggca agtggaatat
    14461 ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca gttggtggcg atcatgttag
    14521 tcgcgacggg caagccggtc gcccgtcgaa actccgccat cacttcacgt ccggagaagc
    14581 cctgttctgc gccgcatgga tcttcggcat aggtaagaac atcattcagc cctttgcata
    14641 aagaaatggc ttcatcaagc agccatgcac cgttgggatc aacggtaatc cgcgcatccg
    14701 ggaagctttt cttcaatgca cgaacagtgt cgatttcttg ctcgccaggt aacaccccgc
    14761 ccttaagttt gaaatcttta aagccatagc gatcctgtga agcttccgcc agacgcacaa
    14821 cggcttcgct gttcatcgct ttctgatggc gcaactgata ccactcatgg ttgcccggcg
    14881 tattttccag ataaggaaga tcggttttgg tccgatcacc tatataaaac agataaccga
    14941 ggacggtaat agtctcgcgt tgcttgcctg gccccaacag ttcgcagacc ggaacattca
    15001 gcgccttacc tagcaggtca agcaaggcgg cttccagcgc cgccacggca ttaacgcgca
    15061 attcaaaagt ccaggcacct ttgccgaagg tctcaaaatc ggctgcctga ttacctttat
    15121 gtacctgctg aaccacttta ttcaggcgcg caacttcctg gcccagaacc atcggaatag
    15181 catcgaccag tgtttgataa atcacctctc cgcccggcgc ttcaccaatg ccggtatgcc
    15241 cggcgttatc ggtgagtacc acgatattgc gggtgaagta tgcgttatgc gcaccaccaa
    15301 tattgagcaa catgctgtca tgtccggcga ccggaatgac tttcatatca gtaataacag
    15361 gactggattg tgtcgccatc ttattgcccc gcgacaggtt tcagctcgat acgcttgata
    15421 tcgcccacca gcaccaggta gctcagtacc gcgattaggg catgaacacc aacataaatc
    15481 agcgccccat taaacgagcc agtcgtgcca acgatataac caattgcgat tggcgtgacg
    15541 atgccagaaa tgttaccgaa catgttgaac tggccaccgg aaagaccgct gatctctttt
    15601 ggcgcggtat ctgccattac tgcccaaccc agcgccccga tgcccttacc gaagaaggcc
    15661 agcgccataa agccgatgat catccactca acgttgacgt agttgcagaa caccatcacc
    15721 atcgacagca acatgcccat tacgatcggt gttttacgcg caatgttcag cgatcccgtg
    15781 cggcgcatca gccagtcgga aataattcca cccagcacac cgccgataaa accgcaaacc
    15841 gccggaacgg aagccacaaa gcccgctttc agaatcgaca tcccacgcgc ctgcaccaga
    15901 taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt tgatacagta ctgaccgata
    15961 taaacgccga tcatcatccg tgacccgagc agctgtttga tctgccccca cttcacgctg
    16021 aacggcactt taactttggt gctttgctga tccatattga tcagcgcgcc acccgcggcg
    16081 atatactcca gctctttctg atttaccccc ggatgttgat ttggctcgtg gatgactttc
    16141 aaccagataa agctgatgac aatgcccaga ccgcccataa agaagaagac gtgtgaccag
    16201 cccacttcat gcgtcagcca gcccataatc ggcgcgaaga tcaccgttgc gaagtattga
    16261 gcggagttaa aaatcgacac cgccgttccc ctttcctgcg ccggaaacca ggccgcaaca
    16321 atgcgactgt tgccggggaa agatggcgct tcagcaagcc cgaccaggaa gcgcagagta
    16381 aacagggcaa cgataatgcc gaatccacta aagatatcga cgaagccttg cagcaaggta
    16441 aacatcgacc agataaagat cgaccagaag tagacgcgtt ttgaaccaaa acgatccagc
    16501 aaccaaccac cagggatctg cccgataaca taagcccatg agaaggcaga gaacacatag
    16561 cccattccca cgggatcaag gccgatatct ttggccattt ccgaaccggc aatagaaagc
    16621 gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca acatcaccac tatccagtaa
    16681 cgagcattcg tgcgtttttc cacactgctc gcagcctgac ttaaagaact cattgttgca
    16741 ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc gacatcgcca ggaaatcaga
    16801 ggtgacgtag ggtgtttttt gccgttttta taggtcgttc gccgaatacg gcgcgtgttt
    16861 atatctggca atagcagtat aaaaagcgcg ccatagcggc tcaccgtgca acaacacaac
    16921 attaatgcgt tcaatgaggc ccgattttgg cattagccct ggacggtgga atccacttca
    16981 cggaaatgaa aacaagaaca agaaaggaag ggtaaaaacg aagaaataaa gaagagtatg
    17041 aaatggatcg cttgactcta ggcaaacgcc agtaaaaatc cgcgctatga agcagttttt
    17101 actggcattt gcctgaaaag attcgattca gcaccgctaa aacgacattt accgctcgct
    17161 gacatatcag gacaacagcg tgccccactg ttcgacccac ggatttgatt ccgtttccgg
    17221 ttccgggttc tcactggcgt caatcaacag catttcgcca acccgctgag cgctctgttc
    17281 ctgcaacaag gcatcgaact gtttgccgcc attgcaaaaa tttacataac tgctgtcgcc
    17341 gagcgcaatc acgccataac gcagattcgg ctggaagccc agactatctt tgattccctg
    17401 aaagagcggc acaatgctat caggaaggtc gccctgcccg gtcgtggacg taaccaccag
    17461 aacatactta tcctgatagg gcagccagtc gcttaattca ggatcttcaa ataccgttgc
    17521 tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc gccactaaca gcgaatttcc
    17581 gtacatggtg ccgacaaaaa taccaatttc cgccataccg ttttctccct ggattaggaa
    17641 cttatctctc catcctgacc cgatggcgca ctgaactcaa ccctttcatt ttcaggaagc
    17701 agaccgcgcc agccaaattg tgataacgcc tgcatccagg tgtcgtccag gcccgcgtga
    17761 atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca actgactggc atgtagcatt
    17821 aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct ggcgtaaatc tccatgtttg
    17881 ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc ggcgaagctg atgtttgcgt
    17941 ccggttttcg gctccagttc caccaggccg tagcgcgtgg tcgggtaacg tccggtcgct
    18001 accggcattt ctacggtcgc cagaccgcga taatgcgtca ctgctggctg cgggccttta
    18061 tcttcgcggg caaatttatc agcgattttg tccagttctt ccaccagtgg ataatccaac
    18121 accgcttctt ccatcaacca gccgcgcaca atcgcatggt aacgtttctg gatttggtgc
    18181 tgttcaaact gttgtgccag cagccgtccg gcctcgctgg ataatcccat caacaacaca
    18241 ccagaagtgg gtcggtccag acgatgagca gtaaaaacat gctgtcctat ctggtcacgc
    18301 acggtttgca tgaccactac tttctcgtcg cgatccagcc agctacggtg aaccagccag
    18361 ccggagggtt tatttaccgc aaccagccat tcatcctgat agagtatttc cagcattagc
    18421 tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc agccagaata agcgcgcgtt
    18481 gcggatggtc cgtcgctagc gccatttcat aatagggtgc aacagcaaaa gcgcccggta
    18541 acggctggtt gttatttaac aaatcgtgca ttcgcgggat cagcccccac tgcaaccact
    18601 ccagtggttc catggtgtcc ataaagaacg gttgggtact attaaattga tgcggctggg
    18661 gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga
    18721 gacgaacgcg gtcatgagtg gtcataaagc aacctcaata agaaaaactg ccgcgaagga
    18781 tagcaggaat aaaaaaaggg agcactgtat tcacagtgct cccggttcgt ttcgcagcat
    18841 tccagctact tttgttgctc cctgctcatc cttgacaact tttcctctgg ccttgcggcc
    18901 aatcgttcat cctgaactat tgcttcctgc tcacaccacc ccgatgtgat acttcatcct
    18961 gaagtatccc tggccttcct gacccaccga atcatcctga ccggttctca ttctccatcc
    19021 tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga agcctttccc
    19081 tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat cctgatgttc acttcgttgt
    19141 ctgactccct gtcgacgaag atagaatcgt ctttttcgcg aagtcttaca aggcgcttac
    19201 agacaatgcc ttaaggaaaa tttcatatga aagttaagaa ttaacaatca acattttgat
    19261 tttaaatgaa aataacgatt cttatctttt taagtattcg cttatttcct ggcgatctcc
    19321 tacagagtct gtaagagatc tctcacaacc agatggggta tttggattac agaagaggct
    19381 taagttgatt aaggaattcc gcaagatttg aggctaaatg cgtccgttta cgtgttccca
    19441 gcgtctcttt gcacacttca ccggagagat tacacaccga gatgacctct aactcttctt
    19501 ccagcgtcgc gataaatagc gtgggcggca gttttagcct tttctgagtc accagatgac
    19561 caatcagatt ttcctgcacc cgacggaagt cgtcttcact ccaggtttgc agcaatgtca
    19621 gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa ctgagtcgta taaaaggtat
    19681 gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac cgcgtttaca ttttgttccc
    19741 cggtaaacgg ctggggttgc cagtatacgg catcttcggt agttgaaata atgcacggtg
    19801 aaggaacacc gtacagttcc tcacttaacg gccagctttt atgctcttca tgccacgcat
    19861 cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt caaatcgtcc actaatttct
    19921 ctcttcactt aaaccagata cacttgtcgt ttagtttatc tggtttatga cggtgaaaca
    19981 tgtcttctta tgcaaaccat caggcacttg cgggcctgac tcttggaaaa tcaaccgatt
    20041 accgggatac ctatgacgcc agcctactgc aaggcgttcc acgcagcctg aatcgcgacc
    20101 cgctgggtct gaaagcggat aacctgcctt ttcacggtac ggatatctgg acgctgtatg
    20161 aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc tgtcggtcat gttgaacttg
    20221 attacaccag cgtaaatctg attgagtcga agagttttaa gctctatctc aacagtttta
    20281 accagacgcg ttttaataac tgggatgagg tgcgccagac gctggagcgc gacttaagca
    20341 cttgcgctca gggtaagatt agcgtggcgt tatatcgtct tgatgaactg gaaggccagc
    20401 cgataggtca tttcaatggc acttgcattg atgaccagga tatcactatc gataactatg
    20461 aattcactac tgactatctg gagaatgcca ccagcggtga aaaagtagtg gaagagacgc
    20521 tcgtcagcca cctgctgaaa tcaaactgcc tgatcaccca tcaaccagat tggggttcga
    20581 tccaaattca gtatcgtgga cgccaaattg acagagaaaa actgctgcgt tacctggtct
    20641 cattccgtca tcacaacgag ttccacgaac agtgcgtgga acgcatcttt aatgacctgt
    20701 tacgcttctg ccagccagag aaattgagcg tttacgcacg ttatacccgt cgtggcggtc
    20761 tggacattaa cccgtggcgc agtaatagcg attttgtccc atcgaccaca agactggttc
    20821 ggcaataaat tttttctcaa ttttgcgtgc tggattcacg cagaaggttg tgaaaggtca
    20881 tcaggcaggg ctattgtaat caaagggaat gacgatattc gtcccataag gagtttttct
    20941 tgattacaca tattagcccg cttggctcca tggatatgtt gtcgcagctg gaagtggata
    21001 tgcttaaacg caccgccagc agcgacctct atcaactgtt tcgcaactgt tcacttgccg
    21061 tactgaactc cggtagtttg accgataaca gcaaagaatt gctgtctcgt tttgaaaatt
    21121 tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct ggaactgatt aatcccccgg
    21181 aagaggcttt tgtcgatggg cgaattattc gcgctttgca ggccaacttg ttcgcggttc
    21241 tgcgagacat tctcttcgtt tacgggcaaa tccataatac cgttcgtttt cccaacctga
    21301 atctcgacaa ctccgtccac atcactaacc tggtcttttc catcttgcgt aacgctcgcg
    21361 cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg gggcggtcac tcaattaacg
    21421 aaaacgagta tttgtatgcc cgtcgcgtcg ggaaccagct gggcctgcgt gagctgaata
    21481 tctgcaccgg ctgtggtcca ggagcgatgg aagcgccgat gaaaggtgct gcggtcggac
    21541 acgcgcagca gcgttacaaa gacagtcgtt ttattggtat gacagagccg tcgattatcg
    21601 ccgctgaacc gcctaacccg ctggtcaacg aattgatcat catgccggat atcgaaaaac
    21661 gtctggaagc gtttgtccgt atcgctcacg gcatcattat cttccctggc ggtgtgggta
    21721 cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa cccggccaac aaagatcagg
    21781 ttttaccatt gatcctcacc ggcccgaaag agagcgccga ctacttccgc gtactggacg
    21841 agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca ttaccgcatc atcattgatg
    21901 acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc gctggtgaaa gaaaatcgcc
    21961 gtgatacagg cgatgcctac agctttaact ggtcaatgcg cattgcgcca gatttgcaaa
    22021 tgccgtttga gccgtctcac gagaatatgg ctaatctgaa gctttacccg gatcaacctg
    22081 ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg tattgtggcg ggtaacgtaa
    22141 aagaagtcgg tattcgcgcc attgaagagt ttggtcctta caaaatcaac ggcgataaag
    22201 agattatgcg tcgtatggac gacctgctac agggttttgt tgcccagcat cgtatgaagt
    22261 tgccaggctc agcctacatc ccttgctacg aaatctgcac gtaatctccc ctcc
//
LOCUS       DADMFL010000064        22227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.064, whole genome shotgun sequence.
ACCESSION   DADMFL010000064 DADMFL010000000
VERSION     DADMFL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.064"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            17..373
                     /locus_tag="JNZ27_002432"
     CDS             17..373
                     /locus_tag="JNZ27_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664734.1"
                     /translation="MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFL
                     GILCGIGMLYFQDWMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAI
                     GMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JNZ27_002433"
     CDS             484..1407
                     /locus_tag="JNZ27_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8664735.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNZ27_002434"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNZ27_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY8664736.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JNZ27_002435"
     CDS             2451..2744
                     /locus_tag="JNZ27_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664737.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNZ27_002436"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNZ27_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY8664738.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JNZ27_002437"
     CDS             complement(4186..4638)
                     /locus_tag="JNZ27_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY8664739.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDVQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /locus_tag="JNZ27_002438"
     CDS             complement(4691..6025)
                     /locus_tag="JNZ27_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8664740.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JNZ27_002439"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JNZ27_002439"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY8664741.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNZ27_002440"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNZ27_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY8664742.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNZ27_002441"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNZ27_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY8664743.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNZ27_002442"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNZ27_002442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY8664744.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNZ27_002443"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNZ27_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY8664745.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /locus_tag="JNZ27_002444"
     CDS             complement(13037..13327)
                     /locus_tag="JNZ27_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY8664746.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNZ27_002445"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNZ27_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY8664747.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNZ27_002446"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNZ27_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY8664748.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JNZ27_002447"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JNZ27_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY8664749.1"
                     /translation="MSMVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JNZ27_002448"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JNZ27_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY8664750.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JNZ27_002449"
     CDS             complement(17349..17846)
                     /locus_tag="JNZ27_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY8664751.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDQGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JNZ27_002450"
     CDS             complement(17843..18205)
                     /locus_tag="JNZ27_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY8664752.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JNZ27_002451"
     CDS             complement(18195..18542)
                     /locus_tag="JNZ27_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY8664753.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JNZ27_002452"
     CDS             18651..19064
                     /locus_tag="JNZ27_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664754.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20577)
                     /locus_tag="JNZ27_002453"
     CDS             complement(19111..20577)
                     /locus_tag="JNZ27_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY8664755.1"
                     /translation="MTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCT
                     PARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGT
                     GECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNR
                     AVDFLQQPVRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPE
                     HHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDH
                     GEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEK
                     PEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDEL
                     YDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPR
                     WMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20588..>22227)
                     /locus_tag="JNZ27_002454"
     CDS             complement(20588..>22227)
                     /locus_tag="JNZ27_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY8664756.1"
                     /translation="TDTGSQQGYFLAGRSLKAPVIAASLMLTNLSTEQLVGLSGQAYK
                     SGMSVMGWEVTSAVTLIFLALIFLPRYLKRGIATIPDFLEERYDKTTRIIIDFCFLIA
                     TGVCFLPIVLYSGALALNSLFHVGESLQISHGAAIWLLVILLGLAGILYAVIGGLRAM
                     AVADSINGIGLVIGGLMVPVFGLIAMGKGSFMQGIEQITTVHAEKLNSVGGPTDPLPI
                     GAAFTGLILVNTFYWCTNQGIVQRTLASKSLAEGQKGALLTAVLKMLDPLVLVLPGLI
                     AFHLYQDLPKADMAYPTLVNNVLPVPLVGFFGAVLFGAVISTFNGFLNSASTLFSMGI
                     YRRIINQNAEPQQLVTVGRKFGFFIAIVSVLVAPWIANAPQGLYSWMKQLNGIYNVPL
                     VTIIIMGFFFPRIPALAAKVAMGIGIISYITINYLVKFDFHFLYVLACTFCINVVVML
                     VIGFIKARATPFTFKDAFAVDMKPWKNVKIASIGILFAMIGVYVGLAEFGGYGTRWLA
                     MISYFIAAVVIVYLIFDSWRHRHDPAVTFTPDAKDSL"
BASE COUNT         5573 a         6000 c         5571 g         5083 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggactgg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaacggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgaa catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc cattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgctactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gagataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctagcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccacagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcatggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ctggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg
    19681 aatacgggtg atgtcgtcat acatcgccgc tcctttactg atcagcttat gtgcgcccat
    19741 catttcaccg tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa
    19801 ggcattgatg acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg
    19861 atggtgataa agcccgtcat caccgactgg cgatggcatc gcctgcgccc ataagcggtg
    19921 atgttccggt ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc
    19981 agcgtatttc tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga
    20041 aaccaccatc aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg
    20101 attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc
    20161 gacgctgttt aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata
    20221 gttcgcccca tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa
    20281 atagtcatga ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt
    20341 aaagtagcgc cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc
    20401 ggactggtta gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga
    20461 acaagtgtag gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt
    20521 attcagcggt ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg
    20581 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    20641 gccgccagct gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca
    20701 tcgctaacca gcgcgtaccg tagccgccga attcagccaa cccgacatag acgccaatca
    20761 tcgcgaacag gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    20821 caaacgcatc tttgaaggtg aacggcgtgg cgcgcgcttt gataaaaccg atcaccagca
    20881 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    20941 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    21001 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    21061 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    21121 acggcgctac caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga
    21181 ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata
    21241 atgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    21301 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    21361 tgtctgcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    21421 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    21481 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    21541 tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga
    21601 ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc
    21661 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa
    21721 taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    21781 ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta
    21841 gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg
    21901 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    21961 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    22021 gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca
    22081 ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt
    22141 tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    22201 agccttgttg cgatccggta tctgttt
//
LOCUS       DADMFL010000065        21359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.065, whole genome shotgun sequence.
ACCESSION   DADMFL010000065 DADMFL010000000
VERSION     DADMFL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.065"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..76
                     /locus_tag="JNZ27_002455"
     CDS             <1..76
                     /locus_tag="JNZ27_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8664757.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..1018
                     /locus_tag="JNZ27_002456"
                     /pseudo
     CDS             92..1018
                     /locus_tag="JNZ27_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1257..1316
                     /locus_tag="JNZ27_002457"
     CDS             1257..1316
                     /locus_tag="JNZ27_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8664758.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="JNZ27_002458"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="JNZ27_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY8664759.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="JNZ27_002459"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="JNZ27_002459"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY8664760.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="JNZ27_002460"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="JNZ27_002460"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY8664761.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /locus_tag="JNZ27_002461"
     CDS             complement(6156..7457)
                     /locus_tag="JNZ27_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY8664762.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="JNZ27_002462"
     CDS             7699..8319
                     /locus_tag="JNZ27_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY8664763.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDGLNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="JNZ27_002463"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="JNZ27_002463"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY8664764.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="JNZ27_002464"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="JNZ27_002464"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8664765.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="JNZ27_002465"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="JNZ27_002465"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY8664766.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="JNZ27_002466"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="JNZ27_002466"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY8664767.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="JNZ27_002467"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="JNZ27_002467"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12937..13780
                     /gene="ttdA"
                     /locus_tag="JNZ27_002468"
                     /pseudo
     CDS             12937..13780
                     /gene="ttdA"
                     /locus_tag="JNZ27_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13777..14382
                     /gene="ttdB"
                     /locus_tag="JNZ27_002469"
     CDS             13777..14382
                     /gene="ttdB"
                     /locus_tag="JNZ27_002469"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY8664768.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14430..15893
                     /locus_tag="JNZ27_002470"
     CDS             14430..15893
                     /locus_tag="JNZ27_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY8664769.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="JNZ27_002471"
     CDS             complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="JNZ27_002471"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY8664770.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17187..17402
                     /gene="rpsU"
                     /locus_tag="JNZ27_002472"
     CDS             17187..17402
                     /gene="rpsU"
                     /locus_tag="JNZ27_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY8664771.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17513..19258
                     /gene="dnaG"
                     /locus_tag="JNZ27_002473"
     CDS             17513..19258
                     /gene="dnaG"
                     /locus_tag="JNZ27_002473"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8664772.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19453..21294
                     /gene="rpoD"
                     /locus_tag="JNZ27_002474"
     CDS             19453..21294
                     /gene="rpoD"
                     /locus_tag="JNZ27_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY8664773.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5152 a         5899 c         5486 g         4822 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact cgccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcggcattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggtg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcaa gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattt agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgagtcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatactgc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacgg actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaagcc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatc gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaacc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11881 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attgacgctt tgtgcatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    13021 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    13081 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    13141 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13201 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13261 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13321 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13381 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13441 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13501 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13561 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13621 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13681 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13741 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaaccccg
    13801 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13861 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13921 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13981 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    14041 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    14101 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    14161 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14221 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14281 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14341 atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctca
    14401 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14461 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14521 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14581 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14641 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14701 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14761 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14821 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14881 tggcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14941 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    15001 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    15061 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    15121 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15181 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15241 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15301 ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga
    15361 tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgaca
    15421 ttgtcagtaa taaagcggca tggaacgttt tcttctggct ggcctcgctt atcaccctcg
    15481 cgaccggact caacaatacc ggttttatta gctggtttgg caaactgtta gcaggcagct
    15541 taagtggtta ttcgccgacg atggtgatgg tgacgttgat tgtggtgttt tatctactgc
    15601 gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg atgatcgccg
    15661 ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggcgcggcaa
    15721 ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta
    15781 gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc
    15841 tcgtgttgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtgagga
    15901 cggtgccgga aattgctctc cggcacccaa tagttttacg cagccggtaa ctccgccagc
    15961 ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc
    16021 attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg cgcgtagaac
    16081 acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt gcggttagca
    16141 ctcacgccgc ccgccatgac cagtcgctta aagcccgtct gatccaacgc tcgcttacac
    16201 ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc
    16261 tggtcgtcgg tgctgttgtc acgaatcgtg ttcgccgcaa aggttttcag accagaaaag
    16321 ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg cccggcagta
    16381 ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc
    16441 gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag cagctcgtac
    16501 tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag cagcgcgaca
    16561 aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg
    16621 tgtaccggga ttgccggaac gttccaggca aacgccagag agcgccccac ggttgcgcca
    16681 accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt
    16741 gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga
    16801 tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta
    16861 tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg
    16921 caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca
    16981 gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta
    17041 tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca
    17101 gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa
    17161 ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt
    17221 tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag
    17281 ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg
    17341 cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact
    17401 aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc
    17461 gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg
    17521 acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat
    17581 cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca
    17641 caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc actgctttgg
    17701 atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc tcgagttcgt
    17761 cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg aagcaggcag
    17821 cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga tggacggtct
    17881 gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc gccagtatct
    17941 ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt ttgcgccccc
    18001 cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat
    18061 tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt tccgcgaacg
    18121 ggtgatattc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct
    18181 gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg
    18241 ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt
    18301 ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc
    18361 gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa
    18421 tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc gagcgctgga
    18481 aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt tgccggatgg
    18541 cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga
    18601 gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag ttgatctgag
    18661 tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc
    18721 gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga
    18781 tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt
    18841 tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga
    18901 attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg
    18961 cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacct ccgggcaact
    19021 tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga
    19081 cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt
    19141 tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt
    19201 aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt
    19261 taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc
    19321 ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca
    19381 gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt
    19441 ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt
    19501 aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc
    19561 gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg
    19621 gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct
    19681 gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac
    19741 ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa
    19801 attgacatcg ctaagcgtat tgaagacggg atcaaccaag ttcaatgctc cgttgctgaa
    19861 tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg
    19921 cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct
    19981 accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa
    20041 gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg
    20101 gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga caccatcaaa
    20161 gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc
    20221 aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg
    20281 atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa
    20341 atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga tacctggttc
    20401 aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa
    20461 gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag aaaccggcct gaccatcgag
    20521 caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag
    20581 aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac
    20641 cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat gaaagcggtt
    20701 gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt
    20761 caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg
    20821 attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt
    20881 gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa
    20941 gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat
    21001 tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc
    21061 accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc gcgtgaagca
    21121 aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct ggaagaagtg
    21181 ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc
    21241 aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga ttaatcggta
    21301 ggccggatca ggcgttacgc cgcacccggc actagtctct ctgcacaaac gccaccttt
//
LOCUS       DADMFL010000066        21254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.066, whole genome shotgun sequence.
ACCESSION   DADMFL010000066 DADMFL010000000
VERSION     DADMFL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.066"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(32..1012)
                     /locus_tag="JNZ27_002475"
     CDS             complement(32..1012)
                     /locus_tag="JNZ27_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY8664774.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JNZ27_002476"
     CDS             complement(1020..1730)
                     /locus_tag="JNZ27_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY8664775.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQQAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNZ27_002477"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNZ27_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY8664776.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="JNZ27_002478"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="JNZ27_002478"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY8664777.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="JNZ27_002479"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="JNZ27_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY8664778.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="JNZ27_002480"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="JNZ27_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY8664779.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /gene="yshB"
                     /locus_tag="JNZ27_002481"
     CDS             8307..8417
                     /gene="yshB"
                     /locus_tag="JNZ27_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY8664780.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNZ27_002482"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNZ27_002482"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY8664781.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNZ27_002483"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNZ27_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY8664782.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11299..11931
                     /locus_tag="JNZ27_002484"
     CDS             11299..11931
                     /locus_tag="JNZ27_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY8664783.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNZ27_002485"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNZ27_002485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY8664784.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15101..15343
                     /locus_tag="JNZ27_002486"
     CDS             15101..15343
                     /locus_tag="JNZ27_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664785.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15463..16395
                     /locus_tag="JNZ27_002487"
     CDS             15463..16395
                     /locus_tag="JNZ27_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8664786.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="JNZ27_002488"
     CDS             complement(16436..17866)
                     /locus_tag="JNZ27_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8664787.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNZ27_002489"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNZ27_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY8664788.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDIFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNZ27_002490"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNZ27_002490"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY8664789.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRSMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="JNZ27_002491"
     CDS             complement(19727..19996)
                     /locus_tag="JNZ27_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY8664790.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="JNZ27_002492"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="JNZ27_002492"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY8664791.1"
                     /translation="MNLMTTITGVVLAGGKARRMAGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="JNZ27_002493"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="JNZ27_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY8664792.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5179 a         5349 c         5125 g         5601 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc tgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agcgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtatag cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaatatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgcg
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg gggttcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatgga
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatggccgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccagcgc aaaaaggccc gactgggcct
    21241 tctgcttaat ttga
//
LOCUS       DADMFL010000067        21134 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.067, whole genome shotgun sequence.
ACCESSION   DADMFL010000067 DADMFL010000000
VERSION     DADMFL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.067"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNZ27_002494"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNZ27_002494"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY8664793.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JNZ27_002495"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JNZ27_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY8664794.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JNZ27_002496"
     CDS             complement(2649..3200)
                     /locus_tag="JNZ27_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY8664795.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JNZ27_002497"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JNZ27_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY8664796.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JNZ27_002498"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JNZ27_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY8664797.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNZ27_002499"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNZ27_002499"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY8664798.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNZ27_002500"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNZ27_002500"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY8664799.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPNARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNZ27_002501"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNZ27_002501"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY8664800.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNZ27_002502"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNZ27_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNZ27_002503"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNZ27_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY8664801.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JNZ27_002504"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JNZ27_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY8664802.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JNZ27_002505"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JNZ27_002505"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY8664803.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNZ27_002506"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNZ27_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY8664804.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNZ27_002507"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNZ27_002507"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY8664805.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNZ27_002508"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNZ27_002508"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY8664806.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNZ27_002509"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNZ27_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY8664807.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JNZ27_002510"
     CDS             complement(18174..18569)
                     /locus_tag="JNZ27_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8664808.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNZ27_002511"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNZ27_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8664809.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINIVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JNZ27_002512"
     CDS             19485..20078
                     /locus_tag="JNZ27_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8664810.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /locus_tag="JNZ27_002513"
     CDS             20223..20675
                     /locus_tag="JNZ27_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8664811.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21134
                     /locus_tag="JNZ27_002514"
     CDS             20798..>21134
                     /locus_tag="JNZ27_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8664812.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERA"
BASE COUNT         5061 a         5273 c         5586 g         5214 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cagggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcact ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccagatga agccattgaa ctggcagccc
     3721 gcgcagaaca ggcggcattg caggctgaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca ttcgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tctaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aagcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgatgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc tgggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcgg
//
LOCUS       DADMFL010000068        20673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.068, whole genome shotgun sequence.
ACCESSION   DADMFL010000068 DADMFL010000000
VERSION     DADMFL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.068"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNZ27_002515"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNZ27_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY8664813.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSSNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNZ27_002516"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNZ27_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY8664814.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNZ27_002517"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNZ27_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY8664815.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNZ27_002518"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNZ27_002518"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY8664816.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JNZ27_002519"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JNZ27_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY8664817.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JNZ27_002520"
     CDS             complement(8217..9110)
                     /locus_tag="JNZ27_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY8664818.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNZ27_002521"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNZ27_002521"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY8664819.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JNZ27_002522"
     CDS             complement(10007..10189)
                     /locus_tag="JNZ27_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY8664820.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JNZ27_002523"
     CDS             complement(10241..11473)
                     /locus_tag="JNZ27_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY8664821.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNZ27_002524"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNZ27_002524"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY8664822.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNZ27_002525"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNZ27_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY8664823.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="JNZ27_002526"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="JNZ27_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNZ27_002527"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNZ27_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY8664824.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNZ27_002528"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNZ27_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY8664825.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNZ27_002529"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNZ27_002529"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY8664826.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVPTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /locus_tag="JNZ27_002530"
     CDS             complement(19788..20552)
                     /locus_tag="JNZ27_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8664827.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4966 a         5713 c         5022 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttactgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcggc acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaaac gccgacaatg tgc
//
LOCUS       DADMFL010000069        20552 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.069, whole genome shotgun sequence.
ACCESSION   DADMFL010000069 DADMFL010000000
VERSION     DADMFL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.069"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="JNZ27_002531"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="JNZ27_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY8664828.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="JNZ27_002532"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="JNZ27_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY8664829.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="JNZ27_002533"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="JNZ27_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY8664830.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="JNZ27_002534"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="JNZ27_002534"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY8664831.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="JNZ27_002535"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="JNZ27_002535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY8664832.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4452..4817
                     /locus_tag="JNZ27_002536"
     CDS             4452..4817
                     /locus_tag="JNZ27_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY8664833.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4967..5485
                     /locus_tag="JNZ27_002537"
     CDS             4967..5485
                     /locus_tag="JNZ27_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY8664834.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5475..6700
                     /gene="dgcN"
                     /locus_tag="JNZ27_002538"
                     /pseudo
     CDS             5475..6700
                     /gene="dgcN"
                     /locus_tag="JNZ27_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6716..7198
                     /locus_tag="JNZ27_002539"
     CDS             6716..7198
                     /locus_tag="JNZ27_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY8664835.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7275..7622)
                     /gene="rplS"
                     /locus_tag="JNZ27_002540"
     CDS             complement(7275..7622)
                     /gene="rplS"
                     /locus_tag="JNZ27_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY8664836.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7664..8431)
                     /gene="trmD"
                     /locus_tag="JNZ27_002541"
     CDS             complement(7664..8431)
                     /gene="trmD"
                     /locus_tag="JNZ27_002541"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY8664837.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEMLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8462..9010)
                     /gene="rimM"
                     /locus_tag="JNZ27_002542"
     CDS             complement(8462..9010)
                     /gene="rimM"
                     /locus_tag="JNZ27_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY8664838.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9029..9277)
                     /gene="rpsP"
                     /locus_tag="JNZ27_002543"
     CDS             complement(9029..9277)
                     /gene="rpsP"
                     /locus_tag="JNZ27_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY8664839.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9414..10775)
                     /gene="ffh"
                     /locus_tag="JNZ27_002544"
     CDS             complement(9414..10775)
                     /gene="ffh"
                     /locus_tag="JNZ27_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY8664840.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10867..11733
                     /locus_tag="JNZ27_002545"
     CDS             10867..11733
                     /locus_tag="JNZ27_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY8664841.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11754..13040
                     /locus_tag="JNZ27_002546"
     CDS             11754..13040
                     /locus_tag="JNZ27_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8664842.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13095..13688)
                     /gene="grpE"
                     /locus_tag="JNZ27_002547"
     CDS             complement(13095..13688)
                     /gene="grpE"
                     /locus_tag="JNZ27_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY8664843.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13811..14689
                     /gene="nadK"
                     /locus_tag="JNZ27_002548"
     CDS             13811..14689
                     /gene="nadK"
                     /locus_tag="JNZ27_002548"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY8664844.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14775..16436
                     /gene="recN"
                     /locus_tag="JNZ27_002549"
     CDS             14775..16436
                     /gene="recN"
                     /locus_tag="JNZ27_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY8664845.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAIPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16585..16926
                     /gene="bamE"
                     /locus_tag="JNZ27_002550"
     CDS             16585..16926
                     /gene="bamE"
                     /locus_tag="JNZ27_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY8664846.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16988..17278)
                     /locus_tag="JNZ27_002551"
     CDS             complement(16988..17278)
                     /locus_tag="JNZ27_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY8664847.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17268..17744)
                     /gene="ratA"
                     /locus_tag="JNZ27_002552"
     CDS             complement(17268..17744)
                     /gene="ratA"
                     /locus_tag="JNZ27_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY8664848.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17876..18358
                     /gene="smpB"
                     /locus_tag="JNZ27_002553"
     CDS             17876..18358
                     /gene="smpB"
                     /locus_tag="JNZ27_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY8664849.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18573..18935
                     /gene="ssrA"
                     /locus_tag="JNZ27_002554"
     tmRNA           18573..18935
                     /gene="ssrA"
                     /locus_tag="JNZ27_002554"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19140..20381
                     /locus_tag="JNZ27_002555"
     CDS             19140..20381
                     /locus_tag="JNZ27_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8664850.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5366 c         4826 g         5055 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcca atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggcg ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcacctcgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcagtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttgccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcattaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgatc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacaccccc ctttgaaaac aatccgctat gctttgaaaa
     4441 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
     4501 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgtcg
     4561 gtatggaaga tgcgatatcg gacagcgcca taaaagatga cgatgcattt agcgattcac
     4621 aggcggatcg cggtctatac cttaaaggat atgccgaagg gcaaaagaaa acttgccaga
     4681 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
     4741 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
     4801 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
     4861 tgttttattt ctcacattga tgacggtcgc atgaaaaaat gatattttaa tgagcatcct
     4921 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
     4981 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaagagcc
     5041 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
     5101 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
     5161 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
     5221 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
     5281 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
     5341 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
     5401 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
     5461 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
     5521 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgttg atctggttgc
     5581 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
     5641 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
     5701 caactgaaac actggcagcg ctgggccagc aagggcattt tcaactgcag aagtacgtga
     5761 taagcagcaa aatattctgg catcctggca ttacccccgt aaggatccag gcgatacttt
     5821 cagcaatttc ataagccact ggctcttccc tgcccccatc attcagccga ttcgtcacaa
     5881 tggtgaaacc attggcgaag tacgcttaac cgctcgcgac agttcaatta gccattttat
     5941 ctggttttcg ctcgccgtac tgaccggttg tattctgctg gcatcaggca tcgcaattac
     6001 cctcacccgc catttgcaca atggcctggt ggaagcactg aaaaatatca ccgatgtcgt
     6061 acatgatgtg cgttccaacc gcaatttttc ccgacgagtt tcggaagaac gtatcgctga
     6121 gtttcaccgc ttcgctctcg acttcaacag tctgctggat gaaatggaag agtggcagct
     6181 tcgtttacag gccaaaaatg cgcagcttct gcgtaccgcg ctacatgacc cattaaccgg
     6241 gctggctaac cgcgcagcgt ttcgtagcgg catcaatacg ttgatgaaca attccgatgc
     6301 ccgaaaaacg tcggcgttac tatttcttga tggcgataat ttcaaatata tcaatgatac
     6361 ctggggtcat gcgacgggcg atagggtctt gattgaaatc gcaaaacggt tagctgaatt
     6421 tggcgggctg cgacataaag cataccgcct gggcggcgat gaattcgcta tggtgctcta
     6481 tgatgtacag tcggaatctg aagtgcagca gatatgctca gcactgacac aaatctttaa
     6541 tctcccgttt gatcttcata atggccatca gaccaccatg acattaagca ttggttacgc
     6601 gatgaccatt gagcacgcct ctgcggaaaa attacaagag cttgccgatc acaatatgta
     6661 tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa caaggatata tcgatatgat
     6721 aaagcacctg gtagcacccc tgattttcac ctcactaata ctgactggct gccagtcccc
     6781 tcagggaaag tttactcctg agcaagtcgc cgctatgcaa tcttatggat ttactgaatc
     6841 cgccggcgac tggtcgctgg gcttatcaga tgccattctg ttcgcaaaaa atgactacaa
     6901 attgctcccg gaaagccagc aacagatcca aaccatggca gccaaattgg cctcgacagg
     6961 gctaacacat gcccgtatgg atggacacac cgataactat ggtgaagaca gttacaacga
     7021 aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca tgggctattg gtggacaaat
     7081 tccacgcagc aatctcacca cacagggttt aggaaaaaaa tatcccatag ccagtaacaa
     7141 gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg gtgattacaa ccccttaaaa
     7201 cacgagcaaa cagacaaaaa aagggccagc caattggcca gcccttctta acaggatgtc
     7261 gcttaagcga aatcttagtt aagacgctct ttgatacgag cagccttacc agtacgctca
     7321 cgcaggtagt acagtttagc tttacgaaca gcaccacgac gtttgacaga aatgctgtca
     7381 actaccggag agtgagtctg gaagacacgc tcaacgcctt cgccgttgga aattttacga
     7441 acagtgaatg cagagtgcag accgcggtta cgaatagcga taaccacgcc ctcgaatgcc
     7501 tgcagacgtt ttttggaacc ttcaacaacc catactttca cttccacggt atcacccgga
     7561 cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa gttgcttaat aatgttgctc
     7621 ataatttaat ctcttatcct gggtaaactg atctctcggg ggcttacgcc atcccatcat
     7681 gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc cagcaacctt gcttgctctt
     7741 cagtcagagc caggttttcc agaagttcag gtcttctaag ccaggtacgg cccagcgact
     7801 gtttcaaacg ccagcgacgt atctcggcat ggttgcccga cagtaacact ggcggaactt
     7861 ccatcccttc taacatctca ggccgcgtat agtgcgggca atccagcaat ccttcagcaa
     7921 aggaatcttc cgttgccgag gcttcatgtc ccagtactcc cggaataaac cgggaaacgg
     7981 agtcaatcag cgtcattgct ggtaactcac caccactgag aacgtaatcg ccgattgacc
     8041 attcttcgtc aatttcggtt tggatcacgc gctcatctat accttcgtag cgaccgcaca
     8101 ccagaatcaa tttttgattc gttgccagtt cgctgacgcc cgcttgatca agcttgcgtc
     8161 cctgtggtga cagataaatc acctttgcgc cttcacccgc cgcggctttt gctgcatgaa
     8221 tggcgtcccg caagggttgc accatcatta acatccccgg tccgccgccg taaggacgat
     8281 cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg actccaactc tggatgctca
     8341 gcaggccatt tttaactgcc cggccagtta ccccgtaatc ggtaattgcg cggaacattt
     8401 caggaaacag gctaattatg ccaatccaca tagcgccgtc ttttaccgtt tatccggtgg
     8461 tttaaaaacc aggatcccaa tctacttcga ttgaacgagt agtgagatcg actttcttga
     8521 taacctgccc atcgaggaac ggtacgagac gttccttgat accaaacgca tctttcaggt
     8581 ttgccttaat gacgagaacg tcattagatc cggtttccat catatcgacg actttaccga
     8641 gatcgtagcc ttcagtggtt actacctggc agcccatcag gtctttccag tagtagtccc
     8701 cctcttcaag ctgaggcagc tgcgatgaat ccacgacaat ttcacaattc gtcagcaggt
     8761 tcgccgcatc acgatcgtca acgcctttca gcttgatgat catgtcctga ttgtggtgct
     8821 tccagctttc cagctggact tgctgccact gacccgcctt ctggataaac cagggctgat
     8881 agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac tctgagccac ccacgaatac
     8941 cgtaagacga acccattttt cccaaaacga tgggatcaac aggtgcttgc gcggtgagtt
     9001 gtttgctcat catgaccacc gtgacagatt aagctgcttt gtttacttct ttgatcagcg
     9061 cagcaacgcg atcagaaata gttgcgccct ggccaaccca gtgagcgatg cgatccagat
     9121 ccaggcgagt gccttcttct ttttcgctag cgattgggtt gaagaaacca acgcgctcga
     9181 tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac aacctggtag aacggacgct
     9241 ttttagcgcc gtgacgtgct aaacgaatag ttaccataac atcctcttgt gtgaataaaa
     9301 caaccggacc ccatcgagga acggagtccg gtgtcatatt aaaagcccga aaattttact
     9361 gatttctgga gaaaatgcaa tcagcagtta ataactctgt cgcataaggt catttagcga
     9421 ccaggaaatc ccggcggcat cattcccttc atgcttctca tcatcttcgc cattccgcct
     9481 ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact gcttcagaag gcggttaacg
     9541 tcctgtacct gcatcccgca accggcggcg atacgacgct tacgcgaacc tttgatgatt
     9601 tctggcttag cgcgctcttt catcgtcatt gagttgatga tggcttccat acgcaccagc
     9661 actttatcgt ccatctgcga cttgacgtta tccgggatct gccccatgcc cggcagtttg
     9721 cccatcaagc tggccatgcc gcccatattt ttcatctggc gcagttgctc aagaaagtcg
     9781 ttgagatcga agccgtcgcc tttttttagc ttgctggcta atttctctgc ctgcgcgcgg
     9841 tcaactttgc tttcgatatc ttcgatcagc gacagtacgt cgcccatgcc gagaatacgc
     9901 gacgcgatgc ggtccggatg gaacggctcc agcgcctcag ttttctcgcc aacaccgagg
     9961 aacttgatcg gtttgccagt gatgtgacga atagagagcg ccgcaccgcc gcgggcatcg
    10021 ccgtccactt tggtcaacac tacgccggta agcggtaacg cttcattgaa tgcttttgcc
    10081 gtattggccg catcctgacc ggtcatggcg tcaaccacaa acagggtttc aaccgggtta
    10141 atcgacgcat ggacttgttt gatctcgtcc atcatcgctt cgtcaacgtg cagacgacca
    10201 gcggtatcca ccagcagcac gtcgtagaat ttcagtttgg cttctttcag cgccgcgtta
    10261 acaatatcaa ccggcttctg accaacatca gaagggaaga aatcaacgcc cacctgctct
    10321 gccagcgtct caagctgttt gattgccgcc gggcgataga cgtcagcaga aaccaccagc
    10381 actttcttct tgtgcttctc gcgcaggaaa ttaccgagtt taccaacgct ggttgtttta
    10441 ccggcacctt gcaggcctgc catcagcacg accgcaggcg gttgcgcagc cagattcagg
    10501 gtctggttct cttcgcccat cgccgcaacc agttcgttac ggacgatttt gacgaactcc
    10561 tgccccggcg tcaggctctt attaacttca tgaccaaccg ctttctcttt tacgcgattg
    10621 ataaactcac gcactaccgg cagagcgacg tccgcctcca gcagcgccat gcgcacttcg
    10681 cgcagcgtat cttttacgtt gtcttcagtg aggcgtccac ggccactgat attgcgcagc
    10741 gtgcgcgaca aacgatcggt taaattatca aacattgtct ctcgcctggg gtggaaacgg
    10801 ttggccgcaa tcgcgacaca tcatcagtat tttgccgcag tataacatga aggcgtcttt
    10861 gttgttatgc aacggttgga gcagcgttca cctgacgcta tactgcttct ctttcttatt
    10921 gctcaaactg tcgacattac tatgcccgtt tttgctctgc tcgcgcttgt cgcctactcc
    10981 gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa acggcggctg gcggcgcatg
    11041 gctattattt ctgcggtcat tgcgctggtc tgccacgcaa tcgctctgga agcccgcatc
    11101 ctgcccgacg gtgatagcgg acaaaacctc agcctgctga acgttggttc attggtcagt
    11161 ttggtgatct gtacggtaat gaccattgtg gcttctcgca atcgtggctg gttgctgcta
    11221 cccattgtct atgcctttgc gcttatcaac ctggcgctgg cgaccttcat gcccaatgaa
    11281 tacatcaccc atctggaagc aacgcctggg atgctggtgc acattggctt atcgctcttt
    11341 tcctatgcca cgctaattat cgccgccctg tacgcgctgc aactggcgtg gattgattac
    11401 caactgaaga acaagaagct ggcatttaac caggaaatgc cgccattgat gagtatcgag
    11461 cgtaaaatgt tccacatcac gcagattggc gtggtgctgc taacgctcac gctttgcact
    11521 ggcctgttct acatgcacaa cctatttagc atggaaaata tcgacaaggc tgtgctctct
    11581 atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac attaccatga aggatggcgt
    11641 ggacgccgcg tcgtctggtt taacgttgcg ggcgcggtaa ttctgacact ggcctacttc
    11701 ggcagccgaa ttgtccagca gttaatcagc taaacccaga aaaggagttt cccctggaac
    11761 acatttctac tactacgttg atcattattc tgatcatcat ggtggtcatt tcagcctatt
    11821 tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta tcgtcttcga catatggcga
    11881 aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct gcgtaagcca gaccgcctga
    11941 taagcctggt gttaatcggc aataacctgg tcaatattct tgcctccgcg ctcggcacta
    12001 ttgtcgggat gcgtttgtac ggcgatgcgg gcgtggcaat tgcgactggt gtgctgactt
    12061 ttgtcgtact ggtttttgct gaggtattgc cgaaaaccat tgccgcgctg tacccggaaa
    12121 aagtcgctta tccgagtagt tttctgctgg ctccgctgca aattttgatg atgccgctgg
    12181 tctggttgct gaatgctatc acccgtatgc tgatgcgcat gatgggtatc aaaaccgata
    12241 tcgtggttag cggctctttg agcaaagaag agttgcgcac tatcgtgcat gaatcgcgct
    12301 cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg gaaaaaatga
    12361 ccgttgatga catcatggtg ccgcgcagtg aaattatcgg tattgatatc aacgatgact
    12421 ggaaatctat tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg ctttaccgtg
    12481 attcgctgga cgacgccatc agtatgctgc gagtacgtga agcctggcgg ttgatgtcgg
    12541 agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc ggacgagatc tattttgtgc
    12601 ctgaaggtac gccgctcagc acgcagttgg taaagtttca gcgcaacaaa aagaaagtcg
    12661 gcctggtcgt caacgagtat ggagacattc aggggctggt gacggttgaa gatattctgg
    12721 aagagattgt cggcgatttc actacgtcga tgtcgccaac acttgccgaa gaggtcacgc
    12781 cgcaaaacga cggttcggtg attatcgatg gcaccgccaa cgtgcgggaa atcaacaaag
    12841 cctttaactg gcatctaccg gaagatgatg cccgcacggt taatggcgtc attcttgagg
    12901 cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat tggcgagtac gatatcgata
    12961 ttctcgacgt acaggacaat atgattaagc aggtaaaagt ttttcctgtg aaaccgctgc
    13021 gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg catgcagggc cgtgaattat
    13081 tacgaaaaca gaaattacgc ttttgctttc gctacagtta ccatcgccgc acgaatcgta
    13141 cgaccattca gcgtataacc cttctgcata atgcccagta cgttacctgg cgcaacgtca
    13201 tcagattcca ccattgcgat agcctgatgc acattcgggt ccagcggcac gttagtttcg
    13261 gcgatcactt caacaccaaa cttacgcaca acatccagca tcgacttcag cgtcagctca
    13321 atgccttcaa ccatcgcaga catatccggg ttagctttat cagccacttc cagcgcacga
    13381 tccaggctat caatcaccgg cagcaattcg ttgatgaatt tctccagcgc gaatttgtgg
    13441 gctttttcaa tatccagttc agtacgacga cgcaggtttt ccatttcggc ttttacgcgc
    13501 aaaataccgt cacgttcacg ggtctgggct tcagccagct gagcttcgag attcgcaatt
    13561 ttttcatcgc gcggatccac ctgctcagca gaagcttctg gctcaactgc ctcaatctct
    13621 tcgtgctgat ccatgataat ttcttccggg gcttgcccct caggcgtttt ctgttcttta
    13681 ctactcatga atttctccgc gtttttttcg cattcatctc gctaacttcg attattatgg
    13741 ggatcagttt cagggtttca agggaagcac tcacattgtc atcaatcttc gcaacaagga
    13801 cctcggaaaa atgaataatc atttcaagtg cattggcatt gtgggacacc cacggcaccc
    13861 tactgcactg acaacacatg aaatgctcta ccgctggctg tgcacaaaag gttacgaggt
    13921 catcgttgag caacaaatcg ctcacgaact gcaactgaag aatgtgaaaa ctggcacgct
    13981 cgcggagatt gggcaactgg ctgatctcgc ggtagtcgtt ggtggcgacg gtaatatgct
    14041 gggcgcggcg cgcacgctcg cccgttacga tattaaagtt attggaatca accgtggcaa
    14101 cctgggtttc ctgactgacc ttgaccccga taacgcccag caacaattag ccgatgtgct
    14161 ggaaggtcac tacatcagcg agaaacgttt tttgctggaa gcgcaagtct gtcagcaaga
    14221 ttgccaaaaa cgcatcagca ccgcgattaa cgaagtggta cttcatcctg gcaaagtggc
    14281 gcatatgata gagttcgaag tgtatatcga cgagatcttt gcgttttctc agcgatctga
    14341 tgggctaatt atttcgacgc caacaggctc caccgcctat tccctctctg cgggcggtcc
    14401 tattctgacc ccctctctgg atgcgattac cctggtgccc atgttcccgc atacgttgtc
    14461 agcacgacca ctggtcataa acagcagcag cacgatccgt ctgcgttttt cgcatcgccg
    14521 taacgacttg gaaatcagtt gcgacagcca gatagcactg ccgattcagg aaggtgaaga
    14581 tgtcctgatt cgtcgctgtg attaccatct gaatctgatt catccgaaag attacagtta
    14641 tttcaataca ttaagcacca agctcggctg gtcaaaaaaa ttattctaat tttacgccag
    14701 cctctttact gtatataaaa ccagtttata ctgtacacaa taacagtaat ggtttttcat
    14761 acaggaaaac gactatgttg gcacaactga ccatcagcaa ctttgctatc gttcgtgagc
    14821 ttgagattga ttttcatagc ggcatgaccg taataactgg cgagaccggt gcgggtaaat
    14881 ctattgcaat agatgccctc ggtctttgtc tcggtggtcg cgctgaagcc gacatggtgc
    14941 gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc tctgaaagat acgccagcgg
    15001 ccctgcgctg gctggaagaa aaccagcttg aagacgggca tgaatgtttg cttcgtcgcg
    15061 tgatcagcag tgatggtcgc tcccgtggtt tcatcaacgg tacagctatt cctctgtcac
    15121 aactgcgcga actgggtcag ttgctgattc agatccatgg tcagcacgct catcaattac
    15181 tcaccaaacc tgagcaccaa aaattcctgc ttgatggcta tgccaatgaa acctctctac
    15241 ttcaggaaat gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac ctcgcgcatc
    15301 atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact gctgcaatac caattaaaag
    15361 aacttaacga atttaatccg cagcccggag agtttgagca aatcgacgaa gagtacaaac
    15421 gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa tgcattggca ttaatggccg
    15481 acggtgaaga cgcaaacctg caaagtcagc tttacacggc taaacaactg gtgagcgaat
    15541 tgattggcat ggacagcaaa ctgtccggcg tacttgatat gctggaagaa gctaccatcc
    15601 agattgctga agccagcgac gaactgcgcc actactgcga tcgtctggat ctcgatccca
    15661 accgactgtt tgaacttgaa cagcgcatct caaaacagat ttcgctggca cgtaaacatc
    15721 acgtcagccc tgaggcatta ccacagtatt accagtcgct actggaagaa cagcagcaac
    15781 tggacgatca ggccgactca caagaaacgc tcgcgctggc ggtaacgaaa catcatcagc
    15841 aggcactgga aaccgcgcgc gcattacacc aacaacgcca gcaatatgca gaagaacttg
    15901 cacagctgat caccgacagt atgcatgcgc tctcaatgcc gcatgggcag tttacgattg
    15961 atgtcaaata tgacgagcat cacctgggcg ctgacggtgc cgatcgtatt gagtttcggg
    16021 taaccaccaa cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc ggtggtgaat
    16081 tgtcccgcat cgcactggca atccaggtca tcacggcgcg taaaatggaa accccggcac
    16141 tgatttttga tgaagtggat gtagggatta gcggtccaac agcggcagtt gtcggcaaac
    16201 tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt tacccacctg ccacaagtcg
    16261 cgggatgtgg tcatcaacac tattttgtca gcaaagaaac cgatggtgcg atgacagaaa
    16321 cacatatgca atctctggat aaaaaagcgc ggttacaaga gctggcgcgc ctgcttggtg
    16381 gcagtgaagt cacacgtaac acactggcga atgcgaaaga actgcttgca gcgtaaactt
    16441 ttttcctgct tcacggtcag agtaaacagc aaaacgccgt aagaccggaa agcaaaaggt
    16501 tttaaagtga tgaaaggtct attatcatcg gcatattaca gatgagccac gtactgctcg
    16561 ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc agcagcagta
    16621 ctattgatgt tgaccgcagg ctgttccact ctggagcgag tggtttaccg tcctgacatc
    16681 aaccagggga actatctgac cgctaacgac gtatccaaaa tacgtgttgg catgacgcaa
    16741 caacaagttg cgtacgcatt gggtacaccg ctgatgtccg atccatttgg tacgaatacc
    16801 tggttctatg tcttccgcca gcaaccaggt catgaaggtg taacgcagca aacgctgacg
    16861 ctgaccttta acagtagcgg tgtgttgacc aatattgata acaaacctgc gctgagtggt
    16921 aactaataat aaagttgctc tcaaagacgt taaaaaaggt gctcaatgag cacctttttt
    16981 ctgtctgtta tttattcgct gatttttctg ctcgttgcct gcgaagctct ttcggatcgg
    17041 caatgagagg gcgataaatc tccacccgat cgccatcatg cacactatcg cttagttttg
    17101 ccggacggct gtaaatgccg actttgtttt tagttaaatc gatatcggta cgcaattcca
    17161 gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc ctcctgcagc gtcactcgct
    17221 gcaggtactg cttctcaggt agcgcataag ccacctcaac ggcaatttta cctagcactg
    17281 taaacctctt tcgcacgaac cgtaaaagcc tggaccatat tagccgccag ctctttaaac
    17341 acgcgaccaa aggcgagttc aatcaactta ttggtaaact caaagtcgag atgaaactcg
    17401 atacgacacg cctcctggct cagcggcgta aacttccatc caccaatcaa tttcttgaac
    17461 ggcccatcca ccagattcat aagaatactt tggttactgg tcaactggtt gcgggtagta
    17521 aacgttttgc tgatcccagc cttagagaca tctaccgccg cagtcatctg cccaggagtg
    17581 gactccagaa tccgacttcc ggtacaacct ggcaaaaact gaggataaga ctgaacgtca
    17641 ttcactaact gatacatttg ctccgcgctg tagggtacca gtgcggtccg gctaatctga
    17701 ggcataacaa tttccatcaa caaaaatcca acaaataata tcatttatcc tgctgtaaaa
    17761 aaaacgctat cccggcgctg ggtaacatcg ggttcatgct aagatagagc cttgtccccc
    17821 gcaggattga tatggggtgt tttcgatttc agattaccga tgattcacga cgcttatgac
    17881 gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg cttaacaagc gcgcccgtca
    17941 cgaatacttt atcgaagaag agttcgaagc gggacttgcc ctgcaaggct gggaagttaa
    18001 atccctgcgc gcaggaaaag ccaatatcag cgacagctac gtccttctgc gtgacggaga
    18061 ggcatttctg tttggcgcta acatcacgcc aatggccgtg gcctccacgc atgtggtgtg
    18121 cgatcctacc cgtacccgca agttacttct caaccagcgc gaactggact cattgtacgg
    18181 tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc ctgtactgga aaaatgcctg
    18241 gtgcaaagtg aaaatcggcg tcgccaaagg taaaaaacag cacgataaac gttcagatat
    18301 caaagaacgc gaatggcagg tggataaagc acgcatcatg aaaaacgccc accgttaaac
    18361 ctgcactcca attattgacc agttcctcac cgcgcctccc tctccggcgg cgcgaatgaa
    18421 catcttattg gctatcacat ccgacacaaa tgttgccatc ccattgctta atcgaataaa
    18481 aatcaggcta catgggtgct aaatctttaa cgataacgcc attgaggctg gtcatggcgc
    18541 tcataaatct ggtatactta cctttacaca ttggggctga ttctggattc gacgggattt
    18601 gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt aaaaagccgc aaaaaatagt
    18661 cgcaaacgac gaaaactacg ctttagcagc ttaataacct gcttagagcc ctctctccct
    18721 agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat cgcgtggaag
    18781 ccctgcctgg ggttgaagcg ttaaaactta atcaggctag tttgttagtg gcgtgtccgt
    18841 ccgcagctgg caagcgaatg taaagactga ctaagcatgt agtaccgagg atgtaggaat
    18901 ttcggacgcg ggttcaactc ccgccagctc caccaaaatt ctccatcggt gattaccaga
    18961 gtcatccgat gaagtcctaa gagcccgcac ggcgcaagcc ctgcgggctt ttttgtgccc
    19021 cgaatttgtc ccgcgaagtc tgatgccaac taattaaatc cgatcctttt aggcaccttg
    19081 ttaggcactt cataaagcta tattgtcttt gaggtgccta aaactatgga aacccggcaa
    19141 tggcaagaca aaccaaacct ctatccgtta aggaaatcga atctgctaaa cccaaggaag
    19201 cggactacgt tctctatgat ggcgatggcc ttgagctact cattaaatcc agcgggagta
    19261 aaatctggca gtttcgctac attcgccctg tcaccaagac acgagcaaag aaaagcatag
    19321 gcccttaccc gtcagttacc cttgccgatg ccagaaacta ccgtgcagag tcccgctcac
    19381 tcctagctaa acagattgat ccgcaggaac atcagcaaga acaattgcgt agttcgctgg
    19441 aagccaaaac caatactttc cagctcgtag ctgaacgttg gtggaatgtg aagaaagcca
    19501 gtgtgacaga ggactatgcg gaagatatct ggcgctctct tgaaagagat gtctttcctg
    19561 cgattggcga cgttagcgtt acagatatta aagctcatac actggttcag gccgtacaac
    19621 cggttcaggc cagaggagca ctggaaacgg ttcgtcgcca gtgtcaacgt attaatgagg
    19681 tcatgatcta tgcccagaac acagggctga ttgatgctgt tcccagcgtt aatatcggta
    19741 aagccttcga aaagcctcag aaaaagaaca tgcccagcat tcgaccggat cagctatctc
    19801 aactgatgca gacaatgcga acagccagca ttagcctttc cacacgctgc ctgttcatgt
    19861 ggcaacttct tactattacc cgccctgccg aagcggctga agctcgctgg gaagaagtag
    19921 acatagaagc gcaagagtgg aagattcccg cagcacgcat gaaaatgaac cgcgaccata
    19981 ctgttccatt gtcagatgaa gcaatagctg tgctggagat gatgaaaccg ctaagcggca
    20041 accgagaatt tatctttcct agccgtatca agcccaacca gcctatgaac agtcagactg
    20101 ttaacgcctc actaaaacgc gcaggttttg gtggtgtgct tgtttcgcat ggtctgagat
    20161 caattgccag tacggctctt aatgaacaag gctttccgcc tgatgccatt gaggcagcat
    20221 tggcccatgt ggataagaat gaggttcgtc gtgcttataa ccgcagcgat tacctggaac
    20281 agcgtcgccc gatgatgcaa tggtgggcaa attttgtgat ggcagcggat cgtggaagca
    20341 tgattgaagg tgggataaaa ggaatgaagc tagttgggtg actaaatagc tgcgcggcat
    20401 agtgtcccac tttcagccag cccttgcagg ttaagccacc agacagtaaa cggcaggtgt
    20461 cgcgttatta ggcgcagcta tttagtagta atattaggat tggtgattct gcactccaga
    20521 agttcggtaa tgctgccaac ttactgattt ag
//
LOCUS       DADMFL010000070        20494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.070, whole genome shotgun sequence.
ACCESSION   DADMFL010000070 DADMFL010000000
VERSION     DADMFL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.070"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JNZ27_002556"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JNZ27_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY8664851.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNZ27_002557"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNZ27_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY8664852.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="JNZ27_002558"
     CDS             complement(977..1267)
                     /locus_tag="JNZ27_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY8664853.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="JNZ27_002559"
     CDS             1701..2411
                     /locus_tag="JNZ27_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY8664854.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTLVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNZ27_002560"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNZ27_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY8664855.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="JNZ27_002561"
     CDS             complement(3539..4198)
                     /locus_tag="JNZ27_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY8664856.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /locus_tag="JNZ27_002562"
     CDS             4351..6684
                     /locus_tag="JNZ27_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8664857.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="JNZ27_002563"
     CDS             complement(6653..7093)
                     /locus_tag="JNZ27_002563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8664858.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLIL
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNZ27_002564"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNZ27_002564"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY8664859.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="JNZ27_002565"
     CDS             7811..8509
                     /locus_tag="JNZ27_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8664860.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="JNZ27_002566"
     CDS             8738..9946
                     /locus_tag="JNZ27_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664861.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="JNZ27_002567"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="JNZ27_002567"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY8664862.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="JNZ27_002568"
     tRNA            12053..12129
                     /locus_tag="JNZ27_002568"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="JNZ27_002569"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="JNZ27_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY8664863.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="JNZ27_002570"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="JNZ27_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY8664864.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNLLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="JNZ27_002571"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="JNZ27_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY8664865.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="JNZ27_002572"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="JNZ27_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY8664866.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="JNZ27_002573"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="JNZ27_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY8664867.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="JNZ27_002574"
     CDS             complement(18744..20015)
                     /locus_tag="JNZ27_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8664868.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4910 a         5069 c         5385 g         5130 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caagaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact ctggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaaggg aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacactgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag tatcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atagcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgatttttg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat acgctccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgaat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatctgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc tggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atga
//
LOCUS       DADMFL010000071        19995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.071, whole genome shotgun sequence.
ACCESSION   DADMFL010000071 DADMFL010000000
VERSION     DADMFL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.071"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..746)
                     /locus_tag="JNZ27_002575"
     CDS             complement(<1..746)
                     /locus_tag="JNZ27_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8664869.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            840..1037
                     /locus_tag="JNZ27_002576"
     CDS             840..1037
                     /locus_tag="JNZ27_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8664870.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1131..1319
                     /locus_tag="JNZ27_002577"
     CDS             1131..1319
                     /locus_tag="JNZ27_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8664871.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1240..1965
                     /locus_tag="JNZ27_002578"
     CDS             1240..1965
                     /locus_tag="JNZ27_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY8664872.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRIVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2077..2424)
                     /locus_tag="JNZ27_002579"
                     /pseudo
     CDS             complement(2077..2424)
                     /locus_tag="JNZ27_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="JNZ27_002580"
                     /pseudo
     CDS             complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="JNZ27_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4431..5540
                     /gene="citC"
                     /locus_tag="JNZ27_002581"
     CDS             4431..5540
                     /gene="citC"
                     /locus_tag="JNZ27_002581"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY8664873.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPHEKQVKNENKPARRCRHP"
     gene            5503..5799
                     /gene="citD"
                     /locus_tag="JNZ27_002582"
     CDS             5503..5799
                     /gene="citD"
                     /locus_tag="JNZ27_002582"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY8664874.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5796..6704
                     /gene="citE"
                     /locus_tag="JNZ27_002583"
     CDS             5796..6704
                     /gene="citE"
                     /locus_tag="JNZ27_002583"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY8664875.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6715..8247
                     /gene="citF"
                     /locus_tag="JNZ27_002584"
     CDS             6715..8247
                     /gene="citF"
                     /locus_tag="JNZ27_002584"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY8664876.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8251..8802
                     /gene="citX"
                     /locus_tag="JNZ27_002585"
     CDS             8251..8802
                     /gene="citX"
                     /locus_tag="JNZ27_002585"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY8664877.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8777..9655
                     /gene="citG"
                     /locus_tag="JNZ27_002586"
     CDS             8777..9655
                     /gene="citG"
                     /locus_tag="JNZ27_002586"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY8664878.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9706..11169
                     /gene="citT"
                     /locus_tag="JNZ27_002587"
     CDS             9706..11169
                     /gene="citT"
                     /locus_tag="JNZ27_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY8664879.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMLLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11283..12089
                     /gene="rna"
                     /locus_tag="JNZ27_002588"
     CDS             11283..12089
                     /gene="rna"
                     /locus_tag="JNZ27_002588"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY8664880.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12318..12728
                     /gene="rnk"
                     /locus_tag="JNZ27_002589"
     CDS             12318..12728
                     /gene="rnk"
                     /locus_tag="JNZ27_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY8664881.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12959..14196)
                     /locus_tag="JNZ27_002590"
                     /pseudo
     CDS             complement(12959..14196)
                     /locus_tag="JNZ27_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14417..14845
                     /gene="uspG"
                     /locus_tag="JNZ27_002591"
     CDS             14417..14845
                     /gene="uspG"
                     /locus_tag="JNZ27_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY8664882.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14966..16531)
                     /gene="ahpF"
                     /locus_tag="JNZ27_002592"
     CDS             complement(14966..16531)
                     /gene="ahpF"
                     /locus_tag="JNZ27_002592"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY8664883.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16776..17339)
                     /gene="ahpC"
                     /locus_tag="JNZ27_002593"
     CDS             complement(16776..17339)
                     /gene="ahpC"
                     /locus_tag="JNZ27_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY8664884.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17711..18457
                     /gene="dsbG"
                     /locus_tag="JNZ27_002594"
     CDS             17711..18457
                     /gene="dsbG"
                     /locus_tag="JNZ27_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY8664885.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18667..19569
                     /locus_tag="JNZ27_002595"
     CDS             18667..19569
                     /locus_tag="JNZ27_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8664886.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19716..19919
                     /locus_tag="JNZ27_002596"
                     /pseudo
     CDS             19716..19919
                     /locus_tag="JNZ27_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5039 a         5146 c         4773 g         5037 t
ORIGIN      
        1 ataacttgcg aaccgcaaag ccagttcccg ttattatcca tgtacgcctg accatcggtg
       61 ccattggctg tccttgagtt attaatcatg tagatgccaa attgcctatt ccccagccca
      121 ccaattacga atttacggtc ggtatggtcc tggcgaagca atgcctgcgc accatcgttg
      181 gataccgcat tacggcccat aataacgttc tggtcacgca tatgaatcca catgcctgta
      241 ctgctgttaa ttaaaaaacg gtttgtaaat atatctcctg taacaaccag accatgcccc
      301 atgcttatcc ggccagttct gagattaagc gtaaaggggc gtaatggccc tatattacca
      361 ttttcccctt cattctctcg tgtagggatg atatgcaggc attcttcaga acgacgaaaa
      421 atggcaccaa aagatgaatt aaatattctc agtgcattga ccgtcgatat ttttacttca
      481 ctgctgaaaa gggctttaac aagaacatac agagcatccc atttaagatt catcaggtct
      541 ttaacccggc cgccagtgag gtggtgtctc catttgaaat actcattgcc gttgtcgcct
      601 gtttcaaacc acatgtatga atcagtatcg ctgtcggcat catttttaaa tccaatcttt
      661 gcccagtcag tattccgaat ccaggcaagg attgagtcgt tttcaaaagt aagtccaccg
      721 gacaacgtat cgccattttt ttgcacggcg ttaccagcct tgtttaccgt ttcctgtaaa
      781 ccgaggggcc gtttctggcc tgcatggcat gatttccgct tttaaacggg gaattcagcg
      841 tgctgattgg ctatgtaagg gtatcaacaa atgaccagaa tacagacctg caacgaaacg
      901 ctcttgtttg tgcaggatgt gaacaaatat ttgaagataa attaagcgga acaaggacag
      961 accgaccggg attaaaacgt gctttaaagc gccttcaaaa aggaacgcat atagaaaacg
     1021 acgatcgaat caattaaatc gatctatatt accgatcgat tatgtcccga taaccacacc
     1081 cgactgatga gattttttaa gataacggcg aattgatgca aggaggtgaa atgaaatcaa
     1141 ttcgctgtaa gaactgcaac aaactgttat ttaaagcgga ttcctttgat cacattgaaa
     1201 tcaggtgtcc gcgttgcaaa cgtcacatca taatgctgaa tgcctgcgag catcccacgg
     1261 agaaacattg tgggaaaaga gaagaaatca cgcattctga cgaaaccgtg cgttattgag
     1321 tatgaaggcc agattgttgg ctatggttca aaggagctgc gcgttgaaac catatcctgc
     1381 tggctggccc gcacaatcat tcagacaaag cactattccc gccgttttgt gaataactct
     1441 taccttcacc tgggagtatt cagcgggcgt gatctggttg gcgttctcca gtggggatat
     1501 gcccttaacc ccaactcagg tcgtcgtatc gtgcttgaaa cggataaccg gggctatatg
     1561 gagttgaatc gcatgtggct acacgacgac atgccccgca actctgaatc acgggccatc
     1621 agctacgcgc tgaaagttat cagattactg tatccgtcag tggagtgggt tcagtccttt
     1681 gctgacgaac gctgcggact cgcaggcgtt gtgtatcagg cgtcgaattt tgattttatt
     1741 ggcagccatg aaagcacgtt ctacgagctg gatggtgagt ggtatcacga gataacgatg
     1801 aacgcgatta agcgcggagg gcaacgaggc gagtatttgc gagctaataa ggagcgtgcc
     1861 gtggtgcaca aatttaatca gtatcgctac atcagattcc tgaacaagcg agcaaggaag
     1921 cggctaaata ccaaactatt caaggttcag ccatacccta agtgatcccc atgtaatgaa
     1981 taaaaagcgg taattaatac tgctgtttca tttgaagcgc gaaagctaaa gttttcgcat
     2041 ttatcgtgaa acgctttcgc gtttttcgtg cgccgcttca cccagccgtt cataggcaat
     2101 tggcttaatg agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc
     2161 actggctgca gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcag
     2221 taaattaatc cctctaccgt caggaagata gttatcgagc aagattagcc ccggcttaaa
     2281 acgctcgatc atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc
     2341 gggaatgtga cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat
     2401 caatagggtt aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg
     2461 taccgcaggg atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg
     2521 caatcaagta caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgacccctg
     2581 ctcaaatatt ttgtctcgta gagactctgg aacgccgcag ccctgatcgg cgacttcaat
     2641 caccacatca tcgccttcat cgctgaggaa taattcaacg ctcttgtttc cttcatcgct
     2701 acgcaggctg gcttcgaagg cgttatcaag taaattgcca acaatggcgg caaactcggt
     2761 gctatccagt cctggcggca gttgcgaaag ctggctaccg gggacgatga tcatttttag
     2821 ccccagttcg cgggcgcgct gcactttacc aaaaagcagc cccgccacct ggcgatcggc
     2881 aaacgcctcg cgcaggctgt caataagctg ttgctgggcc tgagactccc cctgcaccat
     2941 cgccagcacg cgatcatact ctttcatctg caacagacca ttgagagtcg acatccaatt
     3001 gagatgctcg tgtcgcaatg tacgaaggct ctcaacgtat tgttttattt gcgtcagttg
     3061 cgcattgagg gtggaaattt cgtctttact gcgaaagctg ataatggctc ccagcaaatc
     3121 atcacctgaa cgaatagctt cccgattggc aataacgctc agaccgttaa agttagccac
     3181 cacatcctga cgtttttcat caatctgttc ggtaaagaaa tcagcagacc tgaccacttc
     3241 agcaatgggt ttacccaacc attgccgtcc aggggaactc agccccagca tctttcttgc
     3301 gttacgattg atggcggtaa tgtaaccgtg cggatccacc gcaatcagcc cttcataaac
     3361 cgaactaaac agcgcctctt gctgacggac cacccgtgcg atttgctttg gctccatgcc
     3421 catcatctgc cgacggatat gcgcggccag gaaccacgac agcaacatca gaatccctaa
     3481 cagcacgaca aacacacctg ccatcggtaa taaaaactca gcccgccagc tatcgatttt
     3541 actcaccagg tagccaatcg acaccacgcc gatgactttt ccatcgtcat caaagattgg
     3601 cgttttggcg cgcatcgcca tacccattga ccctttcccg gtgatgaagt agctctcccc
     3661 tttctccagc gcgccctgtt tggtgaactg cataggataa ccaattttct ccggattagg
     3721 atggtaaagg cggatcgagt gccggtcgcc aatcaccaca taatcaaaat cggtatctct
     3781 ttgtaatttg ttagcgatgg tcgccagccg tttgtagtca cgcgttttca ccgcagagat
     3841 gacactgtca ttggaggcaa taattttcgc ctgattcatt gccatgtcgc gtacatgaag
     3901 cgttaaatag tcctcaaaac tggccgtaaa atattgcgcc agggctgcaa tgacaaatat
     3961 tgagaacacc agaatcagca aaaagatacg cagcggaaat gtcagtcttt ggaaaaatgc
     4021 aaactgttta ttctcgttaa gcttcaacat tgttttcctt gcgggttgtc ccgagcctct
     4081 ttgacggatg taattaaaat gttgaatcaa aatacataga tattaattat gatcgtggca
     4141 aattttattc attaaatcaa ttaaatcact taaacctgcg agtcatttcg taattacatt
     4201 aattaaaaaa cttaaaacca ttaaacaaat aaaaaaacca ctaaccctat gtgaaataaa
     4261 tcaaaatttc acgccgaaat actccttagg atgtatagcg aaaggagaaa aagatatacc
     4321 tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcagtttgca acattgaaca
     4381 acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt atgttcgtca
     4441 atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa atcgcccaat
     4501 tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt accgtaaccc
     4561 gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa tattattaaa tgcgttgcta
     4621 tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt agccactgaa ctgataaacc
     4681 tcgcctatga gcggcacagc acgcatctgt ttatttatac caaaaccgaa tacgaggcgc
     4741 tgttccgcca gtgcggtttt tccacgctga cctgcgtacc cggcgtgatg gtgctgatgg
     4801 aaaacagcgc cacgcgactg aaacgctatg ccgaatcgct gaaaaaattg cgtcatccag
     4861 ggaacaagat tggctgcatt gtgatgaacg ccaatccctt tacgaatggt caccgttatc
     4921 tgattcaaca agctgcggca cagtgcgact ggtttcatct gtttttagtc aaagaagatt
     4981 cttcacgctt cccctatgaa gaccggctgg atctggtgtt aaaaggcacc gccgatattc
     5041 cacgcctgac tgtgcatcgc ggctcagaat acatcatctc ccgcgctacg ttcccttgct
     5101 acttcattaa agaacagagc gtcattaacc attgttacac cgaaattgat ctgaagattt
     5161 tccgtcagta cctcgctccc gcgcttggtg taactcaccg ctttgtcggt actgaaccct
     5221 tttgtcgcgt taccgcccag tacaaccagg atatgcgcta ctggctggaa acgccgacta
     5281 tctccgcagc gcccatcgaa ctggttgaaa ttgagcggct gcgttaccag gagatgccga
     5341 tatccgcttc ccgggtacgt caactgctgg cgaaaaacga tctcacggct atcgcgccgc
     5401 tggtccctgc agtcacgctg cattatttgc agaacctgct tgaacactcc cgccaggacg
     5461 cggcagctcg tcaaaagacc ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc
     5521 gccgttgcag gcacccttga gtctggggat gtgatgatac gcatcgcccc actcgatacg
     5581 caggatatcg acctgcaaat caatagcagc gttgagaaac agtttggcga tgcaattcgc
     5641 accaccattc tggacgttct cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat
     5701 gacaaaggcg cactggactg cattttacgt gcacgactgg aagccctgct ggcacgcgcc
     5761 agcggtatcc cggctctgcc atgggaggat tgccaatgat ttccgcttcg ctgcaacaac
     5821 gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg tgccaatgcc gcgatggtca
     5881 gcaactcctt catctacccg gctgatgccc tgatgtttga cctcgaagac tccgtggcat
     5941 tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc gctgcaacat ccgctgtatc
     6001 gcgatattga aaccattgtg cgtgtcaacg cgctggattc cgaatggggt gttaacgacc
     6061 tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct gccgaaaacc gataccgctc
     6121 aggatgttct ggatattgaa aaagagatcc tgcgtataga aaaagcctgt ggtcgtgaac
     6181 ccggcagcac cggcctgctg gcggcgattg aatctccgct gggcattacc cgcgcagtgg
     6241 aaatcgctca cgcttccgag cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc
     6301 gcaacctgcg tacagaacgc tccccggaag gaactgaact gctgttcgca cgctgttcca
     6361 ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga taccgtctat tccgacgcta
     6421 acaacgaagc cggattcctg caagaagccg cccacatcaa acagctgggc tttgacggca
     6481 aatcgctgat caacccgcgt cagattgatc tgctgcacaa cctctacgca ccgacccaga
     6541 aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga agccgccgct cgcgaaggcc
     6601 tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc ggttatcgat cgcgcccgtc
     6661 tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga ataaggcaat caaaatgacg
     6721 cagaaaattg aacaatctca acgacaagaa cgggtagcgg cctggaatcg tcgcactgaa
     6781 tgcgatcttg ccgctttcca gaactcacca aagcaaacct accaggctga aaaagcgcgc
     6841 gatcgcaaac tgtgcgccaa cctggaagaa gcgattcgtc gctcaggttt acaggacggc
     6901 atgacggttt ccttccatca cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg
     6961 gacgtcatcg cgaagatggg ctttaaaaac ctgaccctgg cgtccagctc cctgagtgat
     7021 tgccatgcgc cgctggtaga acacattcgc cagggcgtgg ttacccgcat ttatacctct
     7081 ggcctacgcg gtccactggc ggaagagatc tcccgtggtc tgctggcgga accggtgcag
     7141 atccactctc acggcggtcg tgtgcatctg gtacagagcg gcgaactgaa tatcgacgtg
     7201 gctttcctcg gcgtcccgtc ctgtgatgaa ttcggtaatg ccaacggcta caccggtaaa
     7261 gcctgctgcg gctccctcgg ctatgcaatg gttgatgccg acaacgcaaa acaggtcgtg
     7321 atgcttaccg aagaactgct gccttatccg cataatccgg caagcattga gcaagatcag
     7381 gttgatttga tcgtcaaagt tgatcgcgtt ggcgatgctg caaaaatcgg cgctggtact
     7441 acccgtatga ccactaaccc gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt
     7501 gtcaactctg gctacttcaa agaaggtttc tccatgcaaa ccggcaccgg cggcgcttcg
     7561 ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc gcgatattcg cgccgacttc
     7621 gccctcggcg gtattaccgc gacgatggtt gatctgcacg aaaaaggtct gatccgcaaa
     7681 ctgctggatg tgcagagctt tgacagccat gctgcgcaat cgctggcccg taaccccaat
     7741 cacatcgaaa tcagcgccaa ccagtacgct aactggggtt cgaaaggcgc atcggttgat
     7801 cgtctcgacg tggtggtact gagcgcgctg gaaattgaca ggcagttcaa cgttaacgtg
     7861 ctgaccggtt ctgacggcgt actgcgtggc gcttccggtg gtcactgcga taccgcgatt
     7921 gcctctgcgc tttccatcat cgtcgcgcca ctggtacgcg gtcgtattcc gactctggtg
     7981 gataacgtgc tgacctgcat caccccgggc tccagtgtcg atattctggt cacagaccac
     8041 ggtatcgcag ttaacccggc acgtccggaa ctagcagaac gtctgcagga agcgggcatt
     8101 aaagtggttt ccattgagtg gctgcgcgaa cgtgcgcgtc tgctgaccgg tgaaccacag
     8161 ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc
     8221 gatgttgtgc atcaggtgaa ggaataagcc atgcacctgc ttcctgaact cgccagccac
     8281 catgcggtgt caattcccga gctgctcgtc agccgggatg aaaggcaagc acggcaacac
     8341 gcctggctca agcaccatcc tgttccactg gtctccttta ccgtggttgc gcctgggccg
     8401 attaaagaca gcgaggtcac acgccgaatt tttaatcatg gcgtgacagc cttgcgtgcc
     8461 ttagccacaa agcagggctg gcaaattcag gagcaggctg cactggtttc cgccagcggg
     8521 ccggagggca tgttgagcat tgccgccccg gctcgcgatc tcaagctcgc caccattgag
     8581 cttgaacata gtcatcctct cgggcggtta tgggatatcg atgtcctgac gcccgaaggc
     8641 gaaattctct cccgccgcga ctattcactg ccgcctcgcc gctgcctgtt gtgcgagcaa
     8701 agcgcagccg tctgcgcgcg tggaaaaacc catcaactga ccgatttact caaccgcatg
     8761 gaggcactgc tgaacgatgt cgatgcctgc aacgtcaact aaaaccacaa agcttgcgac
     8821 gtcattaatc gatgagtacg ccctgctggg ctggcgcgcc atgctgactg aagtcaatct
     8881 gtcaccgaaa ccaggcctcg tggatcgcat taactgcggt gcgcacaaag atatggcgct
     8941 ggaagatttc caccgcagcg cgctggcgat tcagggctgg ttaccccgtt tcattgaatt
     9001 tggtgcctgt agtgcagaaa tggcaccgga agcggtactc cacggattac gcccaattgg
     9061 tatggcttgc gaaggtgata tgttccgcgc cactgcgggc gtaaacacgc ataaaggcag
     9121 cattttttct ttagggctgc tatgtgcggc aattggccgt ttgcttcagc tcaaccagcc
     9181 ggttacgcca acaaccgttt gttctacggc ggcaagtttc tgccgtggcc tgaccgatcg
     9241 cgaactgcgt accaataatt cacgactgac ggcaggtcaa cggttgtacc agcagcttgg
     9301 ccttaccggc gcacgcggtg aagccgaagc gggttatcca ctggtgatca accacgcctt
     9361 gccgcattac ctcactctgc tggatcaggg gttagatcct gaactggcat tgctcgatac
     9421 cttgctccta ctgatggcta ccaacggcga taccaacgtt gcatcgcgcg gtggcgaggg
     9481 gggcctgcgc tggctacagc gcgaggcgca aacattattg caaaaagggg gcattcgaac
     9541 ccccgccgat ctcgattatc tccggcagtt cgacagggag tgtatcgaac gaaatctcag
     9601 tccaggcggc agtgccgacc tgctgatcct tacctggttt ttagcacaga tttaattatt
     9661 taagcacttg ataaatttgg aaatattaat tttcggagaa cccgtatgtc tttagcaaaa
     9721 gataatatat ggaaactatt ggccccactg gtggtgatgg gtgtcatgct tcttatccct
     9781 gtccccgacg gtatgccgcc gcaggcatgg cattacttcg ctgtgtttgt ggcaatgatt
     9841 gtcggcatga tcctcgagcc aattccggca acagcgatca gttttattgc ggttactatt
     9901 tgcgttattg gcagtaatta cctgctcttt gatgccaaag aattagctga cccagcgttt
     9961 aatgcgcaaa aacaggcgct gaaatggggc ctggctggtt tttccagcac cacggtatgg
    10021 ctggtatttg gcgcatttat ttttgcatta gggtatgaag tttccgggtt aggtcgtcgc
    10081 attgcccttt tcctggtgaa attcatggac aaacgcacgc tgacgttggg ttatgcgatt
    10141 gtcattatcg acattctgct ggcaccgttt acaccgtcca acaccgcgcg taccgggggt
    10201 acggtttttc cggtcattaa aaacctgccg ccgttgttta aatcattccc gaacgatccg
    10261 tccgcgcgtc gtattggcgg ctatttgatg tggatgatgg tcattagtac cagtctgagt
    10321 tcatccatgt ttgtcaccgg tgcggcacca aacatgctgg gtctggagtt cgtcagcaaa
    10381 attgccggta tccagattag ctggttgcag tggttcctct gcttcctgcc ggttggggtt
    10441 atcttgctta tcattgcgcc gtggctttcc tacgtgctgt acaaaccgga aatcacacac
    10501 agtgaagaag tggcaacctg ggcgggtgat gaactaaaaa ccatgggtgc gctgacacgc
    10561 agagagtgga cgctgattgg ccttgtattg ctcagcttag gtttgtgggt atttggcagt
    10621 gaagtcatta atgctactgc ggttggtctg ctggcagttt cgctaatgct ggctctgcac
    10681 gttgtgccgt ggaaagacat tacccgctat aacagcgcat ggaacacgct ggtcaacctg
    10741 gcaactctgg ttgtgatggc taacggcctg actcgttctg gttttattga ctggttcgcc
    10801 ggtaccatga gtacgcacct ggaaggattc tcaccaaacg caacggtgat tgtactggtt
    10861 ctggtgttct actttgcaca ctacctgttt gccagcctgt ctgcgcacac cgcaaccatg
    10921 ctgccggtta ttctggccgt cggtaaaggt attccgggcg taccaatgga acaactgtgt
    10981 atcctgctgg tgctgtctat cggtatcatg ggctgtctga cgccgtatgc aaccggtcct
    11041 ggggtgatta tttacggctg tggctatgtg aaatcaaaag attactggcg tcttggcgca
    11101 atcttcgggg tgatttacat ctctatgttg ctgttggttg gctggccgat tctcgccatg
    11161 tggaactaat acgttcgcca aacgctgaat aaatcatcac gcccgccagg taagcccacc
    11221 tggcgggctt ttttatgatt taatagatag tattcagtgt atgtatgagt tccacaccca
    11281 ttatgaaagc attctgccgt aacgccgcgt tgctcgcggt ttctctgctt cccttatctt
    11341 ctgccaacgc cgtagcgttg caggcaaaac agtatggcga ttttgatcgc tatgtcctgg
    11401 ccctctcctg gcaaaccgga ttttgccaga gtcaacacga tcgaaatcgt aacgaacgag
    11461 atgaatgtcg cctgcaaacc gaaacgacca acaaagctga ttttctgacc gtacatggtc
    11521 tgtggccagg attgcctaaa tcggttgctg cccgtggtgt tgatgaacgc cgctggatgc
    11581 gcttcggttg cgctactcgc ccaatcccga atctaccaga agcacgcgcc agccgaatgt
    11641 gttcatcgcc ggaaaccggg ttatcactgg aaacggccgc taaactaagt gaagtcatgc
    11701 caggagctgg cggacgttcc tgcctggaac gctacgaata tgccaaacac ggtgcctgct
    11761 ttggttttga tccggacgca tacttcggta cgatggtacg cctgaatcaa gaaattaaag
    11821 agagcgaagc cggaaaattc cttgcggata attacggtaa aacagtgagc cgccgtgact
    11881 ttgacgccgc ctttgccaaa agctggggaa aagagaacgt gaaagcagtt aagctaacgt
    11941 gccagggtaa ccctgcgtat ttgactgaaa ttcagatctc gatcaaagct gacgccatca
    12001 acgctccgct ttctgcaaac tcattcttgc cacaacctca cccaggtaac tgtggcaaaa
    12061 cctttgtgat tgataaagcg ggttattaat cctctgattt acaaaacacg ctaattcgtc
    12121 ttccataacc atgccgatta agaattatac ataatgcgct gcattgagga attccctgaa
    12181 tgtgacgcaa atcacttcaa agagtgagca acagtcagta tcatcatgtg agttaaaccc
    12241 tcgccgcctg acggtgaggg ttttcttttg ggtatgttta tctgcgacac tcgcagtacc
    12301 gacgacatgg agtaaaaatg tccagaccaa ctatcatcat taacgacctg gatgccgaac
    12361 gcatcgatat tctgctggag caacccgcct atgctggttt gccaatcgcc gacgcgttaa
    12421 acgcagagtt ggatcgcgcc caaatgtgtt cgccagaaga gatgccacac gacgtggtga
    12481 caatgaacag ccgggttaaa ttccgcaatc ttagagatgg cgaagtgcgt gtgcgcacgc
    12541 tggtgtatcc ggcaaaaatg accgatagca atactcagct ttccgttatg gctccggtag
    12601 gtgccgcact gctggggctg cgcgttggcg attccattca ctgggaactt ccgggcggcg
    12661 ttgcaaccca ccttgaagtg ctggaactcg aataccagcc agaagctgct ggcgactacc
    12721 tgctttaatc ccatctgcac aagcgataac tcgctttgcc cagaggatgc actcgcatcc
    12781 tctttacgca aaatcagcaa tttttcctgg ttctcactgt tcacttttgc tgcaaaatta
    12841 ttccgtttcg tttgtgggaa taaccatggc agaagcattt tacatcctga taggatttct
    12901 gattatggcg gcgattatcg tcatggctgt cctttacctc gaaaaccact cctgacgatc
    12961 atattgttcc ccccggcatc gcattcacca gacctgaaac cgccttctgc gccgcctctg
    13021 cgctttgtgc accgggtacc agaatcacct tacggcactc ctcttcacgt ttttcgaaaa
    13081 tctcatatcc ccgggcggcc tcttcaaacg gcatatagtg ggtaacaatt tcttctggtt
    13141 tcagtaatcc tttctcaatt aacggtaata attctcccag ccatgcgtga acgtgggtct
    13201 gtcccatttt aaacgtcaac cctttatcaa aggcgtcgcc aaacaggaaa ccgtgaataa
    13261 atccagcgta gacgcccggt acgctaacaa tgccgccacg cctgaccgcc gcaatacact
    13321 ggcgcaacgc tttaccgctg ctgccctcca gtttcaggtt agtcagcacc gtttccgtgg
    13381 tgctgccttt cgcttcaaaa ccgacggcgt ctattactgc atccacgccc cggtgacctg
    13441 ccgtttgttc aataattgac tgtgccggat cgctatcttc atcgaaatta atcgggatcg
    13501 cgccgtagcg gtcagcggcg aaacgcaagc ggtagggatg atgatcaaca acaaaaatct
    13561 gttccgcacc gagcaaccgt gcacaggcga ttgtcaacaa tcccacagga ccagcaccat
    13621 agactgcaac gcttgaacct tgttggatct gcacattttt tgctgcctgc catgccgttg
    13681 gcagaatatc agaaaggaaa agggctttat catctgaaag caaaggcggt actttaaacg
    13741 gccccacatt ccctttaggg acgcggacat attccgcctg cccgccagga acgccaccat
    13801 acaggtgact ataaccaaac aatgccgcgg gagcaggtat ctgtttttta ttgagcgcag
    13861 cgcctttacc cgcattggta ttttcgcagg cggcatattg ctgtaatcga cagaaaaaac
    13921 agtcgccaca agcaatgacg aacggaatta ccacccggtc gcctttttgc aaatttttta
    13981 cgtccttccc ggtttcaacg acttccccat aaattcatga ccaaaaatat cgccatgttt
    14041 aacctggggt attttgcctc gataaagatg gaggtcagag ccacagatcg ccgttaccgt
    14101 aatacgcaga ataatatcat ctgcctgttc aatgcccgga tcgggaacat tttctacctg
    14161 aacgtgatgt gggccgtgat aagtcaatgc tttcatgcaa cctccagaga cagttcaaaa
    14221 aagagtcagt ctttaagggt agctatcaca gcgggaaaac gggcggtacc gcaggttttg
    14281 ggagaattcc tggtgttcag tgagaatata ttaatcacag aaaccgttcg taaggaaaaa
    14341 gaaccagaca aatatcaggg ataaatatta taatcatggc gaatgatttg ttcatgatta
    14401 acagggagaa agggttatgt ataaggcaat cattatgcca gttgatgtat ttgaaatgga
    14461 attgagcgac aaagctgttc gccacgctga attcctcgcc caggatgacg gagttattca
    14521 tctacttcac gtactacccg ggtcagccag cctgagcctg caccgttttg ccgctgatgt
    14581 gcgtcgtttt gaagagcatc tgcaacatga agcagaagaa cgtctgcaaa cgatggtcag
    14641 ccacttcacc atcgatcctt cccgcattaa acaacatgtc cgttttggta gcgtgcggga
    14701 tgaagtcaat gagttggcag aagaactggg ggctgatgtt gtagttatcg gttctcgcaa
    14761 cccatcgatt tcgacccatc tgttaggttc taacgcctcg agcgtgatcc gccacgccaa
    14821 tctgcctgta ctggtcgttc gctaacagat aaaagtattt atgccgccct gcctggaaca
    14881 tcccggcagg gctttttaat gccataaaaa agccgccagg tttgaccccg gcggctaagc
    14941 aattgcaggt gaatcttact tcttcttatg cagttttggt gcgaatcagg tagtcaaaag
    15001 cactcagaga ggctttggca ccttcgccag tggcgatgat gatctgcttg tacggaaccg
    15061 tcgtacagtc acccgctgcg aacacgcctt tgacgttggt ttcacatttc gcatcaatga
    15121 taatctcgcc catgcggtta cgttcgactg cgccttccag ccagttggtg ttcgggagca
    15181 gaccaatctg gacaaaaata ccggccagtt cgatgttgtg aatattgccg ctgacacgat
    15241 ctcgatattc cagaccaacg actttgctgc cgtcgccttt cacttccgtg gtttgcgcat
    15301 tcagaataat gtcgacgttt ttcaggctgc gcagtttgtc ctgcagaacc tggtcggctt
    15361 tcatttctgg cgcaaattcc agcagcgtta cgtgctcaac gatacccgcc aggtcgattg
    15421 ccgcttccac gccggagtta ccgccgccga taaccgctac gcgtttgcct ttaaacagcg
    15481 ggccgtcgca gtgcgggcag taggtcacgc ctttagtgcg atactgatct tcgcccggaa
    15541 cgttcatgtt gcgccatttt gcacccgtcg ccacgataat gctgcgtgct ttcagtaccg
    15601 cgccagaagc tgtttcaatc tggtgtagac caccttcaac cgctgccggg atcagtttgc
    15661 tggcgctctg gctgtcgatc acatcaacgt cgtattcatc aacgtgaact ttcagtgcgc
    15721 ctgccaactt ctgcccttca gtcttcggta cagaaatgta gttttcgata tcaacggtat
    15781 cgaggatctg accaccaaaa cgttcgccca tcagaccggt acggatgcct ttacgtgcgg
    15841 agtaaattgc tgccgctgca cccgccgggc cagaaccaac gattaatacg tcataagcat
    15901 cacgcttgtt cagctcttct gccgcacgtt tttccgcgcc agtatcaatt ttggcaacga
    15961 tttcagtcaa cgtcatgcgg ccctgaccaa actctttccc gtttacgaac actgccggaa
    16021 cgcccatcac gttgcgatcg gtgatttcgt tctggaaggt gccgccgtca attgcagtgt
    16081 gcttgatgcg cgggttcaat acgctcatca ggttcagcgc ctgcaccacg tccgggcagt
    16141 tatggcaaga gagcgagtaa taggtttcga attcaaaatc accgtcaata tggcgaatct
    16201 gctccagcag agactgcgct tctttcgacg gatgaccacc ggtccacagc aacgccagta
    16261 ccagcgaggt gaactcgtgg cccagcgggg agcctgcaaa acgtggtccc tggttggaac
    16321 ctgggttggt gatcaggaaa gacggcttac gcactggcaa gctgttatct tctttaaagg
    16381 tgactttgtc tgacagttct gcgatttcag ccaacagttc cttgatttct gccgatttag
    16441 cgctgtcatc cagcgtggca attaactcaa caggcttggt caatttctca aggtaagcct
    16501 tgagttgagt tttcatattt gtgtcgagca tgtttatctc ctgggcttta aacatcatca
    16561 tgcaagcggc ctctgcggct acctggatgc agcttgcatc atggtgcggg gaagaaaatc
    16621 gggtgccgca gcacccgaag aattaggtga atttccgaag tatttcgcgt cagagcaagg
    16681 cgactagcct gtgaatcccc gggagcttac acaagtaagt gaccggggtg agcgggcgac
    16741 gccaacgccg ctatggcgtg aaagacgaag gaaatttaga ttttaccaac caggtccaga
    16801 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
    16861 gaagctacgt actgagctgc tttgatttta cgcagcagat cagacgcgtc acggccaatg
    16921 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
    16981 cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
    17041 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
    17101 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt
    17161 tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
    17221 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
    17281 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
    17341 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa
    17401 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
    17461 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
    17521 atctgaacta ccctttggta tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca
    17581 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
    17641 gctcttacct atgacagtga taggttatgc cttttactcg acttttgcac tgactgaaaa
    17701 gaacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
    17761 agaggaactt ccttccccag taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac
    17821 gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac
    17881 catctacctg actcccgatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg
    17941 tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat
    18001 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat
    18061 tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg
    18121 cccgtgggta gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaagcc
    18181 agaaagcccg gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca
    18241 acaatatgaa gcctctggtt gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga
    18301 gcaaatgaaa gtgttaagtg acaatgagaa actgatggac gatctggggg caaatgtcac
    18361 gccggctatc tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga
    18421 tcagaaaacg cttaatatca ttatgggaaa taaataaaag gtaattcatg gatggttgaa
    18481 ctatatccct gcttttcaac catcctaaaa aagcatgaat tgctcgctct accctaagtg
    18541 gctgtgctat tttagaaaaa cttaatcctg agttaagcaa aaaaacataa tcaataaaaa
    18601 tacatacgca aaaaacattg attaagtgaa tataccatgg aagaaaaata taaaccggag
    18661 tagtgtatgg ccaatctcta cgacttgaaa aagttcgact taaatcttct tgtcatattt
    18721 gagtgtattt accagcattt aagcattagc aaagctgctg aatcactata tatcacccca
    18781 tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat ttaatgaccc tttattcatt
    18841 cgctcaggaa aaggtatcgc gccgacgaca acagggttga atctgcatca ccatcttgag
    18901 aaaaacctca aaggccttga acaaacaatt aatatagtca ataagtccga actaaagaaa
    18961 aactttatta tctacggccc tcagctcatt tcttgctcta ataatagtat gctgatccgc
    19021 tgcctacggc aagatgcctc tgtcgagatt gagtgccatg atatcctcat gtcagcagag
    19081 aatgctgaag aactgttggt tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg
    19141 atcagccgtt ccgtcatttg catgccattg catacaattc gcaatactct aatttgtagt
    19201 aataagcatc cgcgaattac tgacaatagc acttatgaac aaattatggc agaagagttc
    19261 acccaattaa tatccaaatc agcaggagtt gatgatattc aaatggaaat agacgagaag
    19321 tttatgaatc gcaaaatttc atttcgtggt tcctctttgc tgacaataat taatagcatt
    19381 gcagttacag atttactggg aatagtcccc tatgaacttt ataactacca ccgtgacttt
    19441 cttaatttaa aagagataaa actagaacat ccgttacctt caataaagct ttacatttcc
    19501 tataataaat cttctttaaa taatttggtg ttctcccgat ttattgaccg tctaaatgag
    19561 agtttttgat atataactac agacagctac tataactttc atctatttat tcacagctcg
    19621 aatgaaaaat aaaacactct gctttacagt gtttacttta taattaacag tcttatctca
    19681 ctgttcaccc tgtaacgttc agagagcgtc ttcagatgtc tatttataaa attcctcttc
    19741 cactcaatat tctggaagct gcacgagagc gtattaccta gacactcaac accctacccc
    19801 gcgtttgtgt ctcattttct ggcggcaaag actctggttt aatgctgcat ctgacagccg
    19861 aacttgcccg acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat
    19921 gaatcgccac ggataatcta gacacttccg agccgttgat aatactggtt ttcatattct
    19981 gtcggtgaca tctgt
//
LOCUS       DADMFL010000072        19900 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.072, whole genome shotgun sequence.
ACCESSION   DADMFL010000072 DADMFL010000000
VERSION     DADMFL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.072"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            7..375
                     /locus_tag="JNZ27_002597"
     CDS             7..375
                     /locus_tag="JNZ27_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664887.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JNZ27_002598"
     CDS             complement(411..1367)
                     /locus_tag="JNZ27_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY8664888.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNZ27_002599"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNZ27_002599"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY8664889.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JNZ27_002600"
     CDS             complement(2605..3366)
                     /locus_tag="JNZ27_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8664890.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JNZ27_002601"
     CDS             3499..3909
                     /locus_tag="JNZ27_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664891.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JNZ27_002602"
     CDS             complement(3871..4977)
                     /locus_tag="JNZ27_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8664892.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JNZ27_002603"
     CDS             complement(4988..6121)
                     /locus_tag="JNZ27_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8664893.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JNZ27_002604"
     CDS             complement(6114..7850)
                     /locus_tag="JNZ27_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8664894.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNZ27_002605"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNZ27_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY8664895.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNZ27_002606"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNZ27_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY8664896.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10922
                     /gene="rhlE"
                     /locus_tag="JNZ27_002607"
                     /pseudo
     CDS             9741..10922
                     /gene="rhlE"
                     /locus_tag="JNZ27_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNZ27_002608"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNZ27_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JNZ27_002609"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JNZ27_002609"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY8664897.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14877
                     /gene="ybiB"
                     /locus_tag="JNZ27_002610"
     CDS             13915..14877
                     /gene="ybiB"
                     /locus_tag="JNZ27_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY8664898.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15018..16103
                     /locus_tag="JNZ27_002611"
     CDS             15018..16103
                     /locus_tag="JNZ27_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY8664899.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAEGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16332..16592)
                     /locus_tag="JNZ27_002612"
     CDS             complement(16332..16592)
                     /locus_tag="JNZ27_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8664900.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16857..17123)
                     /gene="ybiI"
                     /locus_tag="JNZ27_002613"
     CDS             complement(16857..17123)
                     /gene="ybiI"
                     /locus_tag="JNZ27_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY8664901.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17197..17910)
                     /gene="ybiX"
                     /locus_tag="JNZ27_002614"
     CDS             complement(17197..17910)
                     /gene="ybiX"
                     /locus_tag="JNZ27_002614"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY8664902.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVNGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(17916..19900)
                     /locus_tag="JNZ27_002615"
                     /pseudo
     CDS             complement(17916..19900)
                     /locus_tag="JNZ27_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4615 a         5228 c         5372 g         4685 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca ttggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtagcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ctacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    14101 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    14161 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    14221 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    14281 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    14341 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    14401 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    14461 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    14521 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    14581 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    14641 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    14701 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    14761 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    14821 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    14881 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    14941 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    15001 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    15061 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    15121 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    15181 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    15241 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    15301 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    15361 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggagg
    15421 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    15481 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    15541 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    15601 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    15661 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    15721 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    15781 cgacgcatca ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    15841 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    15901 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    15961 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    16021 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    16081 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    16141 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    16201 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    16261 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    16321 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    16381 gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc
    16441 agggcatcga gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
    16501 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
    16561 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
    16621 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
    16681 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacataca gataaaccat
    16741 ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg
    16801 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
    16861 cgtaactggc tgtctttcga acctctgcga ttatatcctg tatacgctgg tttttgtaaa
    16921 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct
    16981 tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca
    17041 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
    17101 tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg
    17161 tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag
    17221 cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt
    17281 cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
    17341 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
    17401 atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg
    17461 tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
    17521 cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc
    17581 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
    17641 ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc
    17701 attttgcagt gcggcgtata acgcgctgcg ggtgtctact tgctgattgt tcttaacttg
    17761 tgcaccttgt gctccggtgg tgacgcgtcc attcacccat tcggcttgtt ccagttgttc
    17821 gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt
    17881 tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc
    17941 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
    18001 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga
    18061 taccccagtt tggcatctgc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg
    18121 ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca
    18181 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
    18241 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag
    18301 ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg
    18361 acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg
    18421 atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg
    18481 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
    18541 ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
    18601 tagttaatat agacattgcc gttttccgtc aggtgataca gcgccccggc tttccagttc
    18661 accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca
    18721 gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca
    18781 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
    18841 gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
    18901 gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc
    18961 accggattaa cgccatagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca
    19021 tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt
    19081 ttattactca catctttggt attcgccgtg cgtgactagg tccagctatt cacatcgctg
    19141 gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt
    19201 acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa
    19261 cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta
    19321 tgagtatcaa cttttccgga atgattcagg gccgccgctc ccgcagatgg ggcagaatag
    19381 cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga
    19441 taattaagat acaaacgatt cgctgtacca aggccaaaag cgacagaagg ggcgacgccg
    19501 taacgctcat ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc catcacattc
    19561 aggcgcaccg cggtagtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac
    19621 caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg
    19681 atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta
    19741 atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaata
    19801 ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg
    19861 gtggagttac cgttctcacc cgcaaaaaac gcacccacgc
//
LOCUS       DADMFL010000073        19778 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.073, whole genome shotgun sequence.
ACCESSION   DADMFL010000073 DADMFL010000000
VERSION     DADMFL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.073"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..441
                     /gene="feaR"
                     /locus_tag="JNZ27_002616"
     CDS             <1..441
                     /gene="feaR"
                     /locus_tag="JNZ27_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HAY8664903.1"
                     /translation="QESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQ
                     KVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAI
                     RHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(613..942)
                     /locus_tag="JNZ27_002617"
     CDS             complement(613..942)
                     /locus_tag="JNZ27_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY8664904.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(947..1132)
                     /locus_tag="JNZ27_002618"
     CDS             complement(947..1132)
                     /locus_tag="JNZ27_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY8664905.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1129..3768)
                     /locus_tag="JNZ27_002619"
     CDS             complement(1129..3768)
                     /locus_tag="JNZ27_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY8664906.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3976..4965
                     /gene="ldhA"
                     /locus_tag="JNZ27_002620"
     CDS             3976..4965
                     /gene="ldhA"
                     /locus_tag="JNZ27_002620"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8664907.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5076..5412
                     /locus_tag="JNZ27_002621"
                     /pseudo
     CDS             5076..5412
                     /locus_tag="JNZ27_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5409..5675)
                     /locus_tag="JNZ27_002622"
     CDS             complement(5409..5675)
                     /locus_tag="JNZ27_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY8664908.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5949..9473
                     /gene="nifJ"
                     /locus_tag="JNZ27_002623"
     CDS             5949..9473
                     /gene="nifJ"
                     /locus_tag="JNZ27_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY8664909.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9840..10969
                     /gene="ompN"
                     /locus_tag="JNZ27_002624"
                     /pseudo
     CDS             9840..10969
                     /gene="ompN"
                     /locus_tag="JNZ27_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11110..11544
                     /gene="uspF"
                     /locus_tag="JNZ27_002625"
     CDS             11110..11544
                     /gene="uspF"
                     /locus_tag="JNZ27_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY8664910.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11720..12655
                     /gene="ttcA"
                     /locus_tag="JNZ27_002626"
     CDS             11720..12655
                     /gene="ttcA"
                     /locus_tag="JNZ27_002626"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY8664911.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12787..14154)
                     /gene="dbpA"
                     /locus_tag="JNZ27_002627"
     CDS             complement(12787..14154)
                     /gene="dbpA"
                     /locus_tag="JNZ27_002627"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY8664912.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14632..15615)
                     /gene="zntB"
                     /locus_tag="JNZ27_002628"
     CDS             complement(14632..15615)
                     /gene="zntB"
                     /locus_tag="JNZ27_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY8664913.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15870..17102
                     /gene="dgcM"
                     /locus_tag="JNZ27_002629"
                     /pseudo
     CDS             15870..17102
                     /gene="dgcM"
                     /locus_tag="JNZ27_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17123..17686)
                     /gene="smrA"
                     /locus_tag="JNZ27_002630"
     CDS             complement(17123..17686)
                     /gene="smrA"
                     /locus_tag="JNZ27_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY8664914.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SAACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17940..19394)
                     /locus_tag="JNZ27_002631"
     CDS             complement(17940..19394)
                     /locus_tag="JNZ27_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8664915.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     GGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19391..>19778)
                     /locus_tag="JNZ27_002632"
     CDS             complement(19391..>19778)
                     /locus_tag="JNZ27_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8664916.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5196 a         5010 c         4668 g         4904 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt ttgtgcagat
      301 gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca ttggggattt
      361 tctgaccaga gtcatttctc aacggtattt aagcaacgct ttgggatgac gccaggcgag
      421 tatcgccgta aattccgcta actaattggc gttgcatgta ctgcaacgcc aattagttaa
      481 aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga gtttttgaat
      541 attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta tcccgatatt
      601 gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta catattcccc
      661 cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat ctacagacac
      721 attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa tttcggttac
      781 taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa atgtttcacc
      841 tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccag
      901 cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag aaaagatcgc
      961 tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa
     1021 ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg
     1081 tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc
     1141 ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca
     1201 tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg
     1261 atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt
     1321 ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg accggctaat
     1381 ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc
     1441 atctgcggta tctttatcca gccgcaaggt cattggcccg ctatttgcca gccaaccatc
     1501 tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga
     1561 catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag
     1621 ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca cgttaccgcc
     1681 taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc acggataacg
     1741 cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg
     1801 caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag
     1861 gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac
     1921 gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa cctgagcata
     1981 cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt
     2041 cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt
     2101 tacccgaacc gggccaatct caccaacatg taaatcgcct ttaaagagga aataggtcgg
     2161 atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc cgccagtgaa
     2221 cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg
     2281 tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta
     2341 ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc
     2401 accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt ttccccagta
     2461 gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat ccatatgcag
     2521 cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac
     2581 accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga
     2641 atcgatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca gcgttgggtc
     2701 agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt
     2761 cgcttcaccg gtcagctgca gaggcagctg actgttatcc atgcttaatt ttcctgggcc
     2821 aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacac tcagtcgtcc
     2881 gctgaccaga gcgttctcaa gccctgcctg ccagttgtca actttgacat ccagtcggcc
     2941 actcaaagga aaacctgcat acagccagct ccagcgacca tcgcttacgg tcaattgttg
     3001 acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg ccagcacgat
     3061 caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg gttcctgcgg
     3121 caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca cgagcggcat
     3181 agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga catccagttc
     3241 gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata
     3301 acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc attcctgcca
     3361 gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg
     3421 cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc
     3481 agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc
     3541 gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa
     3601 acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca
     3661 gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag
     3721 aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata
     3781 tcctgatttc gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca
     3841 gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatggc
     3901 tttcaattat atttgaaatt ttgtaaaata tttttagcag cttaaatgtg attcaacatc
     3961 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
     4021 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
     4081 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
     4141 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
     4201 tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct gaaagtagtc
     4261 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
     4321 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
     4381 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
     4441 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
     4501 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
     4561 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
     4621 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
     4681 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
     4741 ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
     4801 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
     4861 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
     4921 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     4981 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     5041 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     5101 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaaa
     5161 tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
     5221 gcaggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     5281 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     5341 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     5401 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     5461 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     5521 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaactt
     5581 ggcgctacgt gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat
     5641 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5701 aaacgacacc gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc
     5761 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5821 tcattactct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5881 ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5941 ggtgtcatat gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     6001 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     6061 cctgggccgg aaacggcttg aagaacgttt ggggagacac accacgcgtg gttgaaatgc
     6121 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     6181 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag
     6241 gcgaactgac gccatttgtc ctgcatgtag cggcacgtac cgttgccaca catgcacttt
     6301 ctatttttgg cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt
     6361 gtgcggctaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcaacgctga
     6421 agagccgcgt gccatttatt catttctttg atggttttcg cacgtcccac gaaatcaata
     6481 aaattgtccc gctggccgat gacactattc ttgatctcat gccgcaggcc gaaattgatg
     6541 ctcatcgcgc ccgggcgctc aacccggaac atccggtgat ccgcggtacg tccgccaatc
     6601 ctgacactta tttccagtct cgcgaagcca ccaacccctg gtacaacgcg gtctatgacc
     6661 atgttgaaca ggcgatgaat gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg
     6721 agtattacgg gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca
     6781 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag
     6841 ttcgtctgta ccgtcccttc tccgccaaac acttgctgca agctctgccg ggatccgtac
     6901 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6961 tggatgtgat gaccgcactg gcaggagcct ttaataatgg tgagcgcgaa accctgcccc
     7021 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     7081 cggtatttgc cgagctcaac gcggctaaac ctaaagcacg ctttacggtt ggtatttacg
     7141 atgatgtgac caatctgtca ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac
     7201 ttgaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcaacc aaaaacaata
     7261 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     7321 aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     7381 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     7441 atcagatggc tgagcgttta aaacctagcg gcattttcct gcttaatacg ccgtacagcg
     7501 cagatgaagt gtggtcgcgc ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag
     7561 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta
     7621 ttaataccgt catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg
     7681 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg
     7741 tggagcgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7801 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt
     7861 tcgtgaaaac cgtaaccgcg gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg
     7921 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7981 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg
     8041 cttgcccaca ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc
     8101 ctgccagcct gcattcgcta gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     8161 tgcaggtggc accggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     8221 aagaccgtca gaatccagag attaaagcca tcaatatgat gtctcgccag gaacatgtcg
     8281 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     8341 tggaacgtat tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattcaggtg
     8401 cttgccccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggtgacc
     8461 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     8521 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8581 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8641 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8701 cagacgctac gccagaggtt cgtcgagaac aggtcgcagc tttacgccag caactcaacg
     8761 atgttgccga agcacatgaa ctgctgcgtg acgcagatgc actggtggaa aaatcaatct
     8821 ggctgattgg tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8881 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8941 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taagttcggc gagcacggca
     9001 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     9061 cgcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     9121 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     9181 tggctctcag ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc
     9241 gctttgatcc gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     9301 cgtcagaagc actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc
     9361 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     9421 atgacttcct agcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     9481 ggataaggat tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt
     9541 atatgttgga aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata
     9601 aaggcatata acccgcgcag aataacgtat agaaaattaa aagtataatt tttattttgt
     9661 atgtattatc ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta
     9721 aggttttact taaggcgtaa caaatgatat ttatcggcaa actgaacttc tcctttagga
     9781 tgttttcact ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa
     9841 tgaaaagaaa agtactggca cttttaatcc ctgccctgct cgccgcaggt gctgcacatg
     9901 cagccgaagt ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc
     9961 tgcattattt ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt
    10021 ttaaaggcga aacccaaatt aacgatcaac ttactggcta cggtcaatgg gaatacaata
    10081 ttcaggcaaa caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg
    10141 gactgaaatt tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg
    10201 acatcgaagg ctggaccgat atgctgccta aatttggcgg tgactcttat accaatgcag
    10261 acaactttat gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg
    10321 gtctggtaaa tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta
    10381 atggtcagga aggcaccaat aacgaacgcg atgttcgtca tgaaaacggt gacggctggg
    10441 gtctttccac aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt
    10501 ctgaccgcac caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt
    10561 ggactgctgg gctaaaatac gatgctaaca atatttacct agcaaccatg tattcagaaa
    10621 cgcgtaatat gaccccgttt ggcgacagcg attatgcagt tgcgaacaaa acccagaact
    10681 ttgaagttac tagtaccagt ttgattttgg tctgcgtccg gcagtctctt tcctgatgtc
    10741 taaaggccgt gaccttcacg ctgctggtgg tgcagacaac ccggcaggtg ttgatgataa
    10801 agatctggtt aaatacgccg atgttggcgc gacttactat ttcaataaaa acatgtccac
    10861 ctatgttgac tataaaatca acctgttgga tgaagatgac agtttctacg ctgccaacgg
    10921 catctctacc gatgatattg tcgctttagg tctggtttat cagttctaaa tcctcctgcc
    10981 cgctgttatg gcgggctttt tctgcttatt cttcctcctt tgatctaaat taaaaatgtg
    11041 aactccgtca ttacacaaaa agtgtcatct ggcgttacac tttatgcgga tactaaaaca
    11101 ggaggtttta tgaacagaac gattcttgtc cctatcgata tttcagattc agaattaact
    11161 caacgcgtga ttagccacgt tgaggcagag gcaaagattg atgatgcaga ggttcatttc
    11221 ctgacggtaa taccttcact gccctactat gcctctctgg gtttagcgta ttccgcagaa
    11281 ttaccggcaa tggatgacct gaaagcggaa gccaaatcgc aactggaaga gatcattaaa
    11341 aaatttaaac tgccaaccga cagagtgcat gtccatgttg aggaaggctc gcccaaagac
    11401 cgcattctgg aattggcgaa gaagatcccc gctcatatga tcatcattgc ttcccatcga
    11461 ccggatatca ccacttatct gctcggttcc aacgccgcag ctgtagtgcg tcacgcagag
    11521 tgctccgtgc tggttgtgcg ctgacactaa cgcccgcaca ttgctgcggg ctttttgatt
    11581 catttcgcaa atgtgctgac attttcccct ctaatccgta ccatacacgc cacagttttt
    11641 atatcagatt tcttatgctg agcgttccgg catgacgcat actcttctga tgccatataa
    11701 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11761 cctgaacaaa ttacaaaaac gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa
    11821 tatgattgaa gaaggcgatc gcatcatggt ttgcctctcc gggggtaaag acagctatac
    11881 catgctggag attctgcgca atttgcagca aagcgcgcca atcaattttt cgctggtggc
    11941 tgttaacctc gatcaaaagc aaccgggctt cccggaacac gttctgcccg agtatcttga
    12001 aaagctgggc gttgagtaca agattgttga agagaacact tacggtatcg tgaaagagaa
    12061 gattccagag ggcaaaacca cttgctcact gtgttctcgg ctacgtcgcg gtatccttta
    12121 tcgcaccgca acggaactgg ggacgacgaa gatcgcgttg ggtcaccatc gtgacgatat
    12181 cctgcaaacg ttgttcttaa atatgttcta cggcggcaag atgaaaggta tgcctccgaa
    12241 actgatgagc gatgatggca aacatatcgt tattcgtcca ctggcctact gccgcgagaa
    12301 agatattcag cgatttgccg atgcaaaagc gttcccgatt attccgtgca acctgtgcgg
    12361 ttcacagcct aacctgcaac gtcaggtgat tgctgacatg ttgcgtgact gggataaacg
    12421 ttatccaggg cgtatcgaga cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct
    12481 gtgcgatacc aacctgttcg atttcaaagg cattacccac ggttctgaag tggttaacgg
    12541 gggtgatctg gcgtttgatc gcgaagagat cccactacaa ccggcgggct ggcagccaga
    12601 agaagatgaa aatcagttgg atgagttacg gctgaatgtg gttgaagtga aataaccagg
    12661 atagcgcccg atgcgcaagc gtatcgggct actcttatgg aggccggata agacgcggcc
    12721 agcagcgtcg catccggcaa tcccgaataa gatgtttact cttgcacccg gcaattcaac
    12781 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa
    12841 ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat gcgccggatg
    12901 cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca gtgcacctaa
    12961 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc
    13021 tgcttccagc gtcacaatgg aactattagc tggcggcgtt tgccagttaa gttttatctg
    13081 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga aactgatcgc
    13141 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
    13201 ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac cacgcgcagc
    13261 aacatcagtc gcaaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc
    13321 gcgttgctcc agatcgccgt gtaacgacaa tgcactttgc tctacttcat tcagcgcgtc
    13381 gcagacagcc tggcaatctt ttttggtatt gcaaaacacc acacaagagg atggctgatg
    13441 caagcttaat aaccgttgca acagaggaat tttgccttta ctggatgtct cataaaattg
    13501 ttgttcaatg ggtggcaaag catctgttga gtcaatttca atcgccaaag gatcgcgttg
    13561 cactcgtccg ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg
    13621 tcgagatgca ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc ccatatccag
    13681 catgcggtcg gcctcatcca tcaccaaagt attcagcgca tccagtgata ccgtgccttt
    13741 ttgcaagtga tccagcaaac gacccggcgt tgccacgata atatgcggcg catgttgcaa
    13801 cgaatcacgc tgcataccga acggttgacc accgcacaac gtcaaaattt tggtatttgg
    13861 cagaaaacgc gccagccgac gcaattcacc tgccacctga tccgccagtt cacgcgtagg
    13921 acacagcact aaagcctggg tttgaaatag cgacgcatca atttgctgta acaagccgag
    13981 gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc acgcgaacat cttttccggc
    14041 aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac ccaactcatt
    14101 aaggttcgtg agttgggcgg gaggcaaaac attcagggta gaaaaagcgg tcacaatcta
    14161 ttctcgtggt catcgacgca aagttagcag gcgcgtatcc tcgcagatct acgctcacga
    14221 tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg
    14281 atcggatcgg gcactggaac aggatcgcca ggaatcggtt cagggacagg aatttgcaaa
    14341 taaataagtg tcgtcatatt tccctccggt cattgggtgg actcttaaag ggtagacgct
    14401 gataaataac aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt
    14461 gctatgcagt aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc catcgcttga
    14521 cgttgcattc acctgcaagg gagatattgc cctgaatggg tagagagttt attgacttcg
    14581 ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa aattgctttt gttacaacca
    14641 tttactacgg tgcaaccata aagcaacacc accaataaga acaactaaca gaatacaaaa
    14701 aattgaaaat ccgaattgcc acccgccgcc agggatccca ccaaggttga cgccaaataa
    14761 cccggtcaga aaggtactgg gtaaaaagac cattgccatc aacgacattg tataggtacg
    14821 acgagctaaa ttttcctgca tcacctgagc gatttcatcc gccatcacgc cagtccgtgc
    14881 tatacaggcg tcgatttcgt caaggccgcg cccaaggcga tcggcaatat cctgcatccg
    14941 acggcgttgg tcatcgctca tccacggcaa acgttcactg gcaagacgag cataaacatc
    15001 acgttgcggt gccatatagc gacgcatcac aattaattgt ttgcgcagca gagccaggaa
    15061 tccacgcggt ggaatttgct gatcaaggag attatcttca aggtcgataa ttttatcgtg
    15121 cagctgctcg ataaattcac tggaatgatc ggtcaacgca tcgcacacat ccaccagcca
    15181 tcccccgcaa tcggtcggac ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc
    15241 cagcactttg cgttgtcggg tcgaaacaat taaccgcccg tccatatata cacgcatggc
    15301 gaccagttga tcggggcgtt catcggtgct gccgtttata cagcgcaatg taatcagcgt
    15361 gccttcaccg aaacggctga ctcggggccg tgtgctctcg cccgccagcg catcacgtac
    15421 gttattggga agcagcggtg ttgtcgccag ccattgggcg ctatcatggt gtacataatt
    15481 aaggtggagc cagcagggat gcgcttcatc aatcacatct gtattttcca gcggtttaac
    15541 gccgcctcta ccatccagca tccaggcaaa tactgcatcc gggacattaa cgtccgatcc
    15601 cttaatcgct tccacagtgc ctccatcatc aacgcattat tttgtagtct agccttctgg
    15661 ccctgttacg caacatctca tcaccccatt accctgaaat gattaataaa attctgtcta
    15721 aattgaatac aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc
    15781 gtctatcatt atcagaatta tctgatcata tgacgtggct tttttgcgat cggatagcaa
    15841 caaaaattga taaaaataac gggatctcaa tgattacgca caacttcaat accctggact
    15901 tactcaccag tcctgtctgg attgtttcgc cctttgagga acagttaatt tatgccaata
    15961 gcgcggcgcg actgttgatg caagacctca cgtttagtca gctacgaacc ggaccctatt
    16021 ccgtctcctc acaaaaagaa ctgccgaaat acctctccga tctgcaaaac caacacgata
    16081 ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac agcattaagc tgtcggcttg
    16141 ttttgcgaga gctgacagaa acagaaccgg tgattatttt cgaaggtatc gaagcgccgg
    16201 caacgctggg tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa caaggttttt
    16261 atgcgcgctt ttttctgact aactctgcac caatgttgtt gattgacccg tcacgagatg
    16321 gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta tggttataac catgaaacga
    16381 tgtgccagaa acatacctag gaaataaata tgcttgggcg tcgcgtcatg cctatcatgc
    16441 atgaaatttc gcatttaccc ggcggtcata accctttgaa ttttgttcat aaactggcgg
    16501 atggttctac tcgtcatgtg cagacctatg ccggaccgat tgaaatttat ggcgacaagc
    16561 tcatgttatg tattgtgcat gatattactg agcaaaaacg gctggaggag cagctggaac
    16621 atgctgctca ccatgacgcg atgaccggat tactgaatcg gcgacagttt tatcacatta
    16681 cggaaccagg ccaaatgcag catctcgcca tcgctcagga ttacagcttg ttgctcatcg
    16741 acaccgatcg ttttaaacac attaacgatc tctatgggca ttctaaaggt gatgaggtgt
    16801 tatgcgccct cgcccgcccc ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt
    16861 ggggaggcga agagtttgtc ttattgctac caagaacccc actggatacc gcgctttcgc
    16921 tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta ccacgcttta
    16981 ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa ctgtttaaac
    17041 gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat
    17101 aagccgcgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc cagttttctt
    17161 gcttcgcctg cgccgtttta cgtagtgcga cgtaacacgc cgcgctgccg ccatgatgcg
    17221 gtagcgcggt gcaatatgcc tgaacatcat caaattcggt cagccagcgc gccacatagc
    17281 tgcggacaat attggcatgt gatttatctt cccgcccttt accatgaata atcagcacat
    17341 tacgcaaacc atccgccagg gcttgttgaa tgaaactgaa caccattttg cggcactctt
    17401 ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca ctgcgcagct
    17461 tatccagcac cccatgctgc aacccttcct gccgaaactc cagcggctga cttagcggga
    17521 tgatgtcgag aaatccggta gtgaggaaat tatcaagctg cagcgtgtcg atacgctgcg
    17581 gcgcacgttg gttacgcgtt ggatgccagt ggacatctgt agcacgtttc agcggctgga
    17641 catcttccat tgcgtcaaga aacagcgatt tgtcgtcaag gttcatgtaa cctcctatgc
    17701 accttcacac gtcgtgattc agacgggtta attgttcata cttactgcat cgttcccttc
    17761 acagattgaa acaatactct acttcatacc tgtgagtatc tgcgcaggcg gggcatttta
    17821 tactagcctg ttagcagtat cgaacagttt agcccgagtt taattcagca caggctccga
    17881 tcaggatgat ttattcatag caggaaccaa caaaaatgcg ataaataaca gaccactgat
    17941 tagcagaaag gtaccgaaaa tgccatagtt tgcgcctccc tcagcatcaa ttgactcaat
    18001 aatttggttg aagccagtat ttccgatgtc tggtcacttc tactgcgatt aaaaaataaa
    18061 gcacttctgg aaatgaagtc attcaccgta tggggaacag aaaccatttt ttcacccatt
    18121 ctcgaaaaat gaaaattagt tcgattattg agaatagagc cacacagggc aataccaatc
    18181 gcgcccccaa gattacgtgt taaattaaat acaccagagg ctaacttcag ccgttctttt
    18241 ggaattcccc ccagcgttaa tgttacgatg ggggccatcg caaactgctg caaaatccct
    18301 ctaattgcct gtggaaacag taattcctgc catccccatt cgtgggttat aggggtaaaa
    18361 agatacattg aaaacacaaa tccgcccagc cccgccatca acagccatcg cagatttatt
    18421 tttttactta accagaagta aaagggaaca gaaaataact ggaaaatacc tgttgtgcaa
    18481 acggcaaaac ctatctcttc ggcatttaat ccgcgaatct gccccaaaaa cacaggtatt
    18541 aaatatacag tagagaatat cccaccccct ccagaaaaag aaaaaaaaca tcccagagtg
    18601 aaatttttat ccttaaaagc atgaagatcc attatcggat tggatattgt aagtgtacgc
    18661 gcagcaaata aaataaaact caccaatgcc agaactgatg tcagcaaaat agtattatca
    18721 tccagccatc cccagcgcgc tccttcttca agggtatatt ccagacatcc cagagtcatg
    18781 gccaacagga taatactcgg ataatcagca actttcagta aagagagatc aggtttatca
    18841 aaattaacca gaaaaggaat actcaaaact aaatatatgc caggtaatat attgatataa
    18901 aatagccatc gccagtcgag gttatcagtg agccagcccc ccagagttgg ccctagcgtc
    18961 ggtgacagtg aggccagcgc actgactacc gcagcagcca accccaactc tttaccctgg
    19021 taataaataa acgccgtcgt gaaaactaaa gggatcatgg atgcgccagc agctccctgt
    19081 aaagcacgaa acaaaatcat gatctgaata ttccatgcaa gtccacacgc gatactcatt
    19141 aaagtaaata ttcccgcgga tagagtaaaa agccaacgag tggaaaatac ccgtgacagc
    19201 cacccagaga gcggaataac aataatctca gcaattaaat agctggtctg aagccaggcc
    19261 atttcgtctt tacctgcgga cagaccacca cctatttcat ttagggatga cgatacaatt
    19321 tgaatatcaa gctgagcaat aaataatccc atacacatac tggcgaaagc aatatgttta
    19381 cgcatagaca tcatctctca tcaacactga cggtgacgga cagtcccggg cgaagaagtt
    19441 gctttaattc ctttgcgtcg tcaaatgcaa tccgtaccgg gacacgctga acaattttgg
    19501 tgaaattgcc agtggcattt tctatcggaa tcaagctgaa actggctcct gtcgctgggg
    19561 aaaggctttc aatgtgaccg tgaaacactt cccctttgag aatatcagca cggatctcag
    19621 ccttcattcc tggcttcatt cccgctattt ggttttcttt ataatttgca tcaacccaca
    19681 actcactgac tggtactagt gaaactaacg atgtgccgcc ttccacccaa ctgcctgtat
    19741 gggcgctgcg gtttgcgaca atgccatcaa tcggcgca
//
LOCUS       DADMFL010000074        19385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.074, whole genome shotgun sequence.
ACCESSION   DADMFL010000074 DADMFL010000000
VERSION     DADMFL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.074"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            77..865
                     /gene="modE"
                     /locus_tag="JNZ27_002633"
     CDS             77..865
                     /gene="modE"
                     /locus_tag="JNZ27_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY8664917.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            933..2405
                     /gene="modF"
                     /locus_tag="JNZ27_002634"
     CDS             933..2405
                     /gene="modF"
                     /locus_tag="JNZ27_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY8664918.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2666..3682
                     /gene="galE"
                     /locus_tag="JNZ27_002635"
     CDS             2666..3682
                     /gene="galE"
                     /locus_tag="JNZ27_002635"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY8664919.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3692..4738
                     /gene="galT"
                     /locus_tag="JNZ27_002636"
     CDS             3692..4738
                     /gene="galT"
                     /locus_tag="JNZ27_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY8664920.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4742..5890
                     /gene="galK"
                     /locus_tag="JNZ27_002637"
     CDS             4742..5890
                     /gene="galK"
                     /locus_tag="JNZ27_002637"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY8664921.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5884..6924
                     /gene="galM"
                     /locus_tag="JNZ27_002638"
     CDS             5884..6924
                     /gene="galM"
                     /locus_tag="JNZ27_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY8664922.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7127..7879
                     /gene="gpmA"
                     /locus_tag="JNZ27_002639"
     CDS             7127..7879
                     /gene="gpmA"
                     /locus_tag="JNZ27_002639"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY8664923.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNZ27_002640"
     CDS             complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNZ27_002640"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY8664924.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9414..9794
                     /locus_tag="JNZ27_002641"
     CDS             9414..9794
                     /locus_tag="JNZ27_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664925.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9908..10849
                     /gene="zitB"
                     /locus_tag="JNZ27_002642"
     CDS             9908..10849
                     /gene="zitB"
                     /locus_tag="JNZ27_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY8664926.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10846..11565)
                     /locus_tag="JNZ27_002643"
     CDS             complement(10846..11565)
                     /locus_tag="JNZ27_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY8664927.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNZ27_002644"
     CDS             complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNZ27_002644"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY8664928.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVAIDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(12895..12970)
                     /locus_tag="JNZ27_002645"
     tRNA            complement(12895..12970)
                     /locus_tag="JNZ27_002645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12935..12937),aa:Lys,seq:ttt)
     gene            complement(12974..13049)
                     /locus_tag="JNZ27_002646"
     tRNA            complement(12974..13049)
                     /locus_tag="JNZ27_002646"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13014..13016),aa:Val,seq:tac)
     gene            complement(13101..13176)
                     /locus_tag="JNZ27_002647"
     tRNA            complement(13101..13176)
                     /locus_tag="JNZ27_002647"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13141..13143),aa:Lys,seq:ttt)
     gene            complement(13179..13254)
                     /locus_tag="JNZ27_002648"
     tRNA            complement(13179..13254)
                     /locus_tag="JNZ27_002648"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13219..13221),aa:Val,seq:tac)
     gene            complement(13391..13466)
                     /locus_tag="JNZ27_002649"
     tRNA            complement(13391..13466)
                     /locus_tag="JNZ27_002649"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13431..13433),aa:Lys,seq:ttt)
     gene            complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="JNZ27_002650"
     CDS             complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="JNZ27_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY8664929.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(14432..14953)
                     /gene="pal"
                     /locus_tag="JNZ27_002651"
     CDS             complement(14432..14953)
                     /gene="pal"
                     /locus_tag="JNZ27_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY8664930.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="JNZ27_002652"
     CDS             complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="JNZ27_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY8664931.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQVGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="JNZ27_002653"
     CDS             complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="JNZ27_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY8664932.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="JNZ27_002654"
     CDS             complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="JNZ27_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY8664933.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="JNZ27_002655"
     CDS             complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="JNZ27_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY8664934.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="JNZ27_002656"
     CDS             complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="JNZ27_002656"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY8664935.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
BASE COUNT         4688 a         5132 c         4780 g         4785 t
ORIGIN      
        1 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
       61 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
      121 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
      181 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
      241 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
      301 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
      361 ccagcaaaaa gcctttgatg tgttaagtga tgatgacgcc ctgccgctga acagcctgct
      421 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
      481 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
      541 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
      601 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
      661 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
      721 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
      781 aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
      841 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
      901 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
      961 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     1021 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     1081 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     1141 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     1201 tgctcggccc tagcgaagat gacaccggac gcactacggc tgagattatt caggatgaag
     1261 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     1321 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     1381 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     1441 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     1501 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     1561 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     1621 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     1681 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     1741 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     1801 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     1861 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     1921 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     1981 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     2041 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     2101 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     2161 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     2221 aggggcttga tccgctcaat cgccaactta ttcgccgttt tgttgatgtg ctgattagcg
     2281 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     2341 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     2401 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     2461 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     2521 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     2581 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     2641 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     2701 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     2761 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     2821 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     2881 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     2941 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     3001 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     3061 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     3121 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     3181 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     3241 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     3301 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     3361 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     3421 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     3481 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     3541 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     3601 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     3661 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     3721 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     3781 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     3841 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     3901 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     3961 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     4021 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     4081 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     4141 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     4201 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     4261 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     4321 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     4381 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     4441 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     4501 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     4561 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     4621 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     4681 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     4741 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     4801 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     4861 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     4921 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     4981 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     5041 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     5101 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     5161 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     5221 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     5281 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
     5341 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     5401 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     5461 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
     5521 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
     5581 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     5641 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     5701 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     5761 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     5821 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     5881 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
     5941 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
     6001 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
     6061 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta taccaaccgc
     6121 atcgccaata gtcgttatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
     6181 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
     6241 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
     6301 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
     6361 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
     6421 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
     6481 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
     6541 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
     6601 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
     6661 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
     6721 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
     6781 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
     6841 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
     6901 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
     6961 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca
     7021 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
     7081 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
     7141 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
     7201 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
     7261 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
     7321 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
     7381 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
     7441 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
     7501 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
     7561 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
     7621 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
     7681 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
     7741 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
     7801 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
     7861 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
     7921 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
     7981 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
     8041 aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta
     8101 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
     8161 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
     8221 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
     8281 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
     8341 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
     8401 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
     8461 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
     8521 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
     8581 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
     8641 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
     8701 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
     8761 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
     8821 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgagtg
     8881 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
     8941 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgagc aaccgtattc
     9001 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
     9061 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gtcgccatac
     9121 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
     9181 tttacactat gaacggatta atcctagaat tagcaagaaa aacgccagat tgtcgcgaaa
     9241 aacgagatct ttcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
     9301 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
     9361 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
     9421 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
     9481 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
     9541 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
     9601 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
     9661 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
     9721 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
     9781 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
     9841 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
     9901 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
     9961 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    10021 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    10081 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    10141 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    10201 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    10261 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    10321 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    10381 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    10441 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    10501 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    10561 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    10621 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    10681 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    10741 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    10801 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    10861 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    10921 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    10981 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    11041 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    11101 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    11161 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    11221 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    11281 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    11341 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    11401 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    11461 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    11521 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    11581 taacgttacg ccttattatt cgttatccac gtagtgtaac cgcaaaatcc agcatacgat
    11641 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    11701 cttcctgttc taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat
    11761 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    11821 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc gattgccaca ataagcctct
    11881 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    11941 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    12001 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    12061 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    12121 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    12181 aagttaccac ccaatctgcg cgcgttttta ccgcagcaga agtgttggcg tagacgacga
    12241 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    12301 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    12361 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    12421 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    12481 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    12541 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    12601 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    12661 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg gtgtaatttg
    12721 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    12781 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    12841 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    12901 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    12961 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg
    13021 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    13081 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    13141 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    13201 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    13261 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    13321 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acattttact accgcttcca
    13381 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    13441 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    13501 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    13561 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    13621 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    13681 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    13741 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    13801 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    13861 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    13921 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    13981 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    14041 catcagctgt cggcgtcgtt gatgccgccg caccgctttg atcgctgctg gttgattgcg
    14101 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    14161 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    14221 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    14281 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    14341 cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    14401 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    14461 tggagtatgc tgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    14521 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    14581 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    14641 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    14701 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    14761 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    14821 catccatacc agtgccagca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    14881 tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct ttcagcactt
    14941 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    15001 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    15061 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    15121 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    15181 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    15241 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    15301 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accgacctgg
    15361 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    15421 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    15481 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    15541 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    15601 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    15661 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    15721 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    15781 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    15841 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    15901 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    15961 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    16021 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    16081 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    16141 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    16201 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    16261 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    16321 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    16381 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    16441 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    16501 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    16561 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    16621 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    16681 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    16741 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    16801 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    16861 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    16921 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    16981 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    17041 gttgccgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    17101 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    17161 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg cagcttcttc cgcagcttta
    17221 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    17281 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    17341 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    17401 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    17461 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    17521 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    17581 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    17641 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    17701 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    17761 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    17821 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    17881 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    17941 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    18001 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    18061 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg
    18121 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    18181 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    18241 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    18301 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    18361 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
    18421 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    18481 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    18541 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    18601 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    18661 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    18721 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    18781 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    18841 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    18901 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    18961 tgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    19021 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    19081 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    19141 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    19201 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    19261 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    19321 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    19381 agaga
//
LOCUS       DADMFL010000075        18179 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.075, whole genome shotgun sequence.
ACCESSION   DADMFL010000075 DADMFL010000000
VERSION     DADMFL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.075"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..2089
                     /gene="pbpC"
                     /locus_tag="JNZ27_002657"
     CDS             <1..2089
                     /gene="pbpC"
                     /locus_tag="JNZ27_002657"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY8664936.1"
                     /translation="YLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTL
                     TMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGTAS
                     WAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWLERAEAARNKVLERMAAQGVWSRE
                     QVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKG
                     RLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGL
                     ALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYG
                     PKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQ
                     PDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIG
                     VNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRG
                     VICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRV
                     AADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRD
                     GAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMD
                     DVGQIATVKFVMQ"
     gene            2239..2670
                     /gene="ndk"
                     /locus_tag="JNZ27_002658"
     CDS             2239..2670
                     /gene="ndk"
                     /locus_tag="JNZ27_002658"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY8664937.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2820..3974
                     /gene="trmG/rlmN"
                     /locus_tag="JNZ27_002659"
     CDS             2820..3974
                     /gene="trmG/rlmN"
                     /locus_tag="JNZ27_002659"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY8664938.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4259..5272
                     /gene="rodZ"
                     /locus_tag="JNZ27_002660"
     CDS             4259..5272
                     /gene="rodZ"
                     /locus_tag="JNZ27_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY8664939.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5299..6417
                     /gene="ispG"
                     /locus_tag="JNZ27_002661"
                     /gene_synonym="gcpE"
     CDS             5299..6417
                     /gene="ispG"
                     /locus_tag="JNZ27_002661"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY8664940.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6528..7802
                     /gene="hisS"
                     /locus_tag="JNZ27_002662"
     CDS             6528..7802
                     /gene="hisS"
                     /locus_tag="JNZ27_002662"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY8664941.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7820..8440
                     /locus_tag="JNZ27_002663"
     CDS             7820..8440
                     /locus_tag="JNZ27_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY8664942.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8451..9629
                     /gene="bamB"
                     /locus_tag="JNZ27_002664"
     CDS             8451..9629
                     /gene="bamB"
                     /locus_tag="JNZ27_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY8664943.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9747..11219
                     /gene="der"
                     /locus_tag="JNZ27_002665"
     CDS             9747..11219
                     /gene="der"
                     /locus_tag="JNZ27_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY8664944.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11289..11504
                     /locus_tag="JNZ27_002666"
     CDS             11289..11504
                     /locus_tag="JNZ27_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664945.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11501..12871)
                     /gene="xseA"
                     /locus_tag="JNZ27_002667"
     CDS             complement(11501..12871)
                     /gene="xseA"
                     /locus_tag="JNZ27_002667"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY8664946.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13033..14499
                     /gene="guaB"
                     /locus_tag="JNZ27_002668"
     CDS             13033..14499
                     /gene="guaB"
                     /locus_tag="JNZ27_002668"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY8664947.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14568..16145
                     /gene="guaA"
                     /locus_tag="JNZ27_002669"
     CDS             14568..16145
                     /gene="guaA"
                     /locus_tag="JNZ27_002669"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY8664948.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16240..16779)
                     /locus_tag="JNZ27_002670"
     CDS             complement(16240..16779)
                     /locus_tag="JNZ27_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664949.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEDGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16795..17313)
                     /locus_tag="JNZ27_002671"
     CDS             complement(16795..17313)
                     /locus_tag="JNZ27_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8664950.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17624..17815)
                     /locus_tag="JNZ27_002672"
     CDS             complement(17624..17815)
                     /locus_tag="JNZ27_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY8664951.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17833..17985
                     /locus_tag="JNZ27_002673"
     CDS             17833..17985
                     /locus_tag="JNZ27_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664952.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4298 a         4454 c         4995 g         4432 t
ORIGIN      
        1 ttaccttgaa gcgctgatca attatgaaga tcgctggttc tggaagcatc cgggggttaa
       61 tccattctcg gtggcgcgcg cagcatggca agatctcact tcgggacggg ttatttccgg
      121 tggcagcacg ctcactatgc aggttgctcg tctgcttgat cctcacccca aaacatttgg
      181 tggcaaaatt ctccagctct ggcgcgcgtt gcaactggag tggcatttgt ctaagcgtga
      241 aattctgatc ttgtatctta accgcgctcc gtttggcggt acgttgcagg ggatcggtac
      301 ggcaagttgg gcttatctcg gaaaatcgcc tgcgaattta agctattccg aggcggcaat
      361 gctggcggtg ttaccgcaag tgcccagccg actgcgcccg gatcgttggc tggagcgtgc
      421 cgaagccgcg cgtaataaag tgctcgaacg gatggctgcg caaggtgtgt ggtcccgtga
      481 gcaggtaaaa gagtcaaggg aagaacccat ctggctggca ccccgacaaa tgccgcaact
      541 ggcaccgctg ttttcgcgca tgatgctcgg taaaagcaaa agcgacaaaa tcgttactac
      601 gctggatgcc ggtcttcaac gacgtctgga agaactggcg caaaactgga aagggcggtt
      661 gccaccgcgc agctcactgg cgatgatcgt ggttaatcat accgatatgc gtgttcgcgg
      721 ctgggtggga tcggttgatc tcaacgatga ttcacgcttt ggtcatgttg atatggtcaa
      781 tgcgatccga tcgccaggat cggtgctcaa accgtttgtt tatggtctgg cgctggatga
      841 aggcttgatc cacccggcat cactgctgca agacgtcccc cggcgcaccg gtgattatcg
      901 accaggtaac tttgatagcg gttttcatgg cccgatcaac atgagcgagg cgctggtgcg
      961 ctcgctgaac ttacctgctg tgcaggtgct ggaagcctat ggaccgaaac ggtttgcggc
     1021 aaagttacgc aatgttggat tgccgttata tttgcccaac ggcgctgcgc cgaatctttc
     1081 actcattctc ggcggcgctg gtgcaaaact ggaagatatg gcggcagcgt ataccgcgtt
     1141 tgctcgccac ggcaaggcag gcaaattgcg cttacagcct gacgatccgc tgcttgaacg
     1201 gcctttaatg tcgtcggggg cggcgtggat cattcgccgg attatggctg atgaagcgca
     1261 acccttgccg gatagtgcct tgccgcgcgt cgccccactg gcatggaaaa cgggcaccag
     1321 ctatggctat cgtgacgcct gggcgattgg ggttaacgct cgctatgtca ttgggatctg
     1381 gactggcaga cctgacggca cgcccgttgt tggtcagttt ggctttgcca gtgccgtacc
     1441 attgttgaat caggtcaata atatcttact gtcgcgcagt gcgaatctgc cggaagaccc
     1501 gcgcccgaac tcagtcactc gtggcgttat ctgctggccg ggcggacagt ctttgccgga
     1561 aggtgacggt aactgtcgcc gccgcctggc aacctggctg ctggatggca gccagccgcc
     1621 aacgctgtta ctgtcagagc aggaagggat taatggcatt cgcttcccca tctggctgga
     1681 tgacaaaggg cagcgcgtcg ccgctgactg cccgcaggcg aagcaagaaa tggtaaatgt
     1741 ctggccgcta ccgctggaac cctggctgcc cgcatcagaa cgtcgcgctg tgcgcttacc
     1801 accggcctcg acaatttgcc cgccatacgg tcacgatgcc cagctccctc tgcagctgac
     1861 aggcgttcgc gatggcgcga ttattaaacg tttaccgggc gcagcggaag cgactttgcc
     1921 gttgcaatca agtggagggg caggtgaacg ctggtggttt ctgaatggcg aaccgttaac
     1981 tgaacgcggg cgcaacgtga ccctgcattt gacggataaa ggtgattatc aattgctggt
     2041 gatggatgat gtggggcaaa tcgcgacagt gaaatttgtc atgcaatagt ctgttttgtc
     2101 cctgattgtt gctaaaactc attttatttt aaaaaaaatg ttaccttcat caatagtcaa
     2161 cggccctgtt gctcattata atccgcgcca tctcgtacgc tggtacagac aacaacagaa
     2221 caatttacag aggtaaaaat ggctattgaa cgtacttttt ccatcatcaa accgaacgcg
     2281 gtagcaaaaa acgtcattgg taatatcttt gcgcgctttg aagctgcagg gttcaaaatt
     2341 gtcggcacca aaatgctgca cctgaccgtt gaacaggcac gtggctttta tgctgaacac
     2401 gatggaaaac cgttctttga tggtctggtt gaattcatga cctctggccc gatcgtggtt
     2461 tccgtgctgg aaggtgaaaa cgccgttcag cgtcaccgcg atctgctggg cgcgaccaat
     2521 ccggcaaacg cactggctgg tactctgcgc gctgattacg ctgacagcct gaccgaaaac
     2581 ggtacccacg gttctgattc cgtcgaatct gccgctcgcg aaatcgctta tttcttcggc
     2641 gaaggcgaag tgtgcccgcg cacccgttaa taatttcgta aatgccgcgt gcaaacgtgg
     2701 catccgtgcg ccagaatttg tacaatgcag cgcccccgga cgagcagccg ctcaccgggg
     2761 cgtttctttt ttcaaccctc caggggccat aacgtgtaat aacgaggccg gaatagcata
     2821 tgtctgaaca attagtcaca cctgaaaacg tcaccacgaa agatggaaaa atcaacctgc
     2881 tggatctcaa ccgtcagcag atgcgggagt tttttaaaga tttaggtgaa aaaaccttcc
     2941 gcgccgatca ggtgatgaag tggatgtatc actattgctg cgacaacttt gatgagatga
     3001 ccgacatcaa caaagtgttg cgcggcaaac tgaaagaggt ggcggaaatc cgcgcaccgg
     3061 aagtggttga agaacagcgt tcatccgacg gcaccattaa atgggcgatc gccgttggcg
     3121 atcagcgcgt cgaaacggtg tatatcccgg aagacgaccg tgccacgctc tgcgtctctt
     3181 cgcaggtggg gtgtgcgctg gagtgtaaat tctgttccac cgcccagcag ggctttaacc
     3241 gcaacctgcg ggtatcggaa attattggcc aggtgtggcg tgcggcgaaa atcgtcggcg
     3301 cagcaaaagt caccggtcag cgcccaatca ctaacgtcgt catgatgggc atgggcgagc
     3361 cgctgctcaa cctgaacaac gtcgttccgg cgatggaaat catgcttgat gatttcggtt
     3421 ttggcctgtc taaacgtcgc gtgacgcttt ccacttccgg tgtagttccg gcgctggata
     3481 aactgggcga tatgatcgac gttgcactgg caatttccct gcacgcgccg aacgacgaaa
     3541 ttcgtgacga aattgtgccg atcaacaaaa agtacaatat cgaaacgttc cttgctgcgg
     3601 tgcgccgtta tctggagaaa tccaacgcca atcagggccg agtcactatt gaatacgtga
     3661 tgcttgacca cgttaacgac ggcactgaac acgcgcacca actggcggaa ctgctgaaag
     3721 atacgccgtg taagatcaac ctgatcccgt ggaacccgtt cccgggcgcg ccgtatggac
     3781 gcagttcgaa cagccgtatc gaccgcttct caaaagtgct gatgagctac ggtttcacca
     3841 ctattgtgcg taaaacgcgt ggtgatgata tcgatgctgc ctgtggtcag ttggcgggcg
     3901 atgttatcga ccgtacgaaa cgtactctgc gtaaacggat gcagggtgaa gccatcgata
     3961 ttaaagcggt ctgatagcca ttacgccacg gttactttat gtaccgtggc gtaatctttt
     4021 atgaatgaag tattaagttg caattgtctg gtcaatatta acggtgcgtt tttgctgact
     4081 ttaaggcagt atgtaacgat gcaacagtaa gttagcctca gtgaatgtgg gctttgtcac
     4141 gagcacacag acggtcttat actgtatgat aacggttaac ttaacggatg tttcgcggtg
     4201 tgggtgagca tgatggttca ccggcatctc aattctcatt taaacgtacc tgcagcgaat
     4261 gaatactgaa gccacgcacg accaaaatga agcacttact accggcgctc gcctgcgtaa
     4321 tgctcgcgaa caactaggac ttagtcagca ggccgttgcc gagcgacttt gcctgaaggt
     4381 ttccacggta cgcgacattg aagaagataa ggcacccgcc gatcttgctt caacattcct
     4441 gcgcggatat atccgctctt atgcgcgtct ggtacatatt ccagaagaag aactgctgcc
     4501 agggctggaa aagcaggctc cacttcgggc tgcaaaagtt gcgccaatgc agagtttttc
     4561 cctcggtaaa cgccgcaaaa aacgcgacgg ctggctgatg accttcacct ggctggtgtt
     4621 gtttgtggtt atcggcctga gcggtgcctg gtggtggcaa gaccacaaag ctcagcagga
     4681 agagatcacc actatggccg atcaatcttc ggcggaactg agcagtaata gcgagcaggg
     4741 gcagagtgtt ccgttaaata cgtcgacaac tacagacccg gctacaacgt ccacgccgcc
     4801 agcgtctgtg gatactaccg caaccaacac gcaaacacct gccgtaactg cgccagcacc
     4861 agctgttgat ccgcaacaga atgcggttgt ttcgccttcg caggcaaatg ttgataccgc
     4921 cgcgaccccg gtaccaacgg cagcaacaac gccagatggt gctgcgccgt tgccaaccga
     4981 tcaggctggc gtgaccacgc cggcggctga tccgaatgcg ctggtgatga actttactgc
     5041 cgattgctgg ctggaggtca ctgatgctac cggtaaaaaa ttgtttagcg ggatgcagcg
     5101 taaagacggt aatttgaatt taaccggcca ggcaccgtat aaactgaaaa ttggtgcgcc
     5161 agccgcagta cagatccagt atcaagggaa acctgtcgat ctgagtcgtt ttatcagaac
     5221 taaccaggtt gcgcgtctga ccctcaatgc cgaacaatca ccggcgcagt aacagacggg
     5281 taacgcggga gatttttcat gcataaccag gctccaattc aacgtagaaa atcaacacgt
     5341 atttacgttg ggaatgtgcc gattggcgat ggtgctccca tcgccgtaca gtccatgacc
     5401 aatacgcgta cgacagatgt cgaagcaacg gtcaatcaaa tcaaggcgct ggaacgcgtt
     5461 ggcgctgata tcgtccgtgt ctccgtaccg acgatggacg cggcagaagc gttcaaactc
     5521 atcaaacagc gggttaacgt gccgctggtg gctgacatcc acttcgacta tcgcattgcg
     5581 ctgaaagtag cggaatacgg cgtcgattgt ctgcgtatta accctggtaa tatcggtaat
     5641 gaagagcgta ttcgcatggt ggttgactgt gcgcgcgata aaaacattcc gatccgtatt
     5701 ggcgttaacg ccggatcgct ggaaaaagat ctgcaagaaa agtatggcga accgacgccg
     5761 caggcgttgc tggaatccgc catgcgtcat gttgatcatc tcgatcgcct gaacttcgat
     5821 cagttcaaag tcagcgtgaa agcgtctgac gtcttcctcg ctgttgagtc ttatcgtttg
     5881 ctggcaaaac agatcgatca gccgctgcat ctggggatca ccgaagccgg tggtgcacgc
     5941 agcggggcag taaaatccgc cattggttta ggtctgctgc tgtcggaagg catcggcgac
     6001 acgctgcgcg tatcgctggc ggccgatccg gtcgaagaga tcaaagtcgg tttcgatatt
     6061 ttgaaatcgc tgcgtatccg ttcgcgaggg attaacttca tcgcctgccc gacctgttcg
     6121 cgtcaggaat ttgatgttat cggtacggtt aacgcgctgg agcaacgcct ggaagatatc
     6181 atcactccga tggacgtttc gattatcggc tgcgtggtga atggcccagg tgaggcgctg
     6241 gtttctacac tcggcgtcac cggcggcaac aagaaaagcg gcctctatga agatggcgtg
     6301 cgcaaagacc gtctggacaa caacgatatg atcgaccagc tggaagcacg cattcgtgcg
     6361 aaagccagtc agctggacga agcgcgtcga attgacgttc agcaggttga aaaataataa
     6421 cgtgatggga agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt
     6481 gagggaagcg ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca
     6541 ttcaagccat tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca
     6601 ttgaaggcac actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga
     6661 ttgtagagca gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa
     6721 aagagatgta cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga
     6781 cggcgggctg tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc
     6841 tgtggtatat cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt
     6901 tccatcagtt gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga
     6961 ttatgctcac tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc
     7021 tgaattctat cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc
     7081 ttgagcagca taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc
     7141 tgcgcgtgct ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat
     7201 taggtgatta tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg
     7261 agagcgcggg gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata
     7321 accgtaccgt tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag
     7381 gcggtcgtta tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt
     7441 ttgcgatggg cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag
     7501 ccgatcctgt tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta
     7561 tggcattagc tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg
     7621 gcggcggcaa ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg
     7681 tggtgctggg tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg
     7741 gtgagcaaac ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt
     7801 aaggaaggag aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt
     7861 taaacgcttt tttgctgaaa atggcaaagc actggctgtt ggggtgattt tgggcgttgg
     7921 cgcactgatt ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc
     7981 tcttgcctat caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc
     8041 ggcggaaaaa tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact
     8101 tgcgcagcaa tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg
     8161 gctggcagac acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt
     8221 tcaggtacag ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga
     8281 agggtgggct gccattgttg ccgacctgcg tggtgaagca ttgctgagca aaggtgataa
     8341 gcaaggtgcg cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag
     8401 cgaaatgatg cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc
     8461 gtaaattact gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta
     8521 acagcgaaga agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc
     8581 cgaccacggc gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc
     8641 atccggcact ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc
     8701 tgaatgcgga tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt
     8761 tctctaaaga gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca
     8821 ttggcagcga aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc
     8881 aaactacagt cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa
     8941 tccacaccag taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga
     9001 cagttaacct cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt
     9061 ttggtgcggc cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg
     9121 gccagatgat ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc
     9181 tgagcgatgt tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata
     9241 atggtaacct gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg
     9301 gttcggtgaa tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc
     9361 gggtgatggc gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc
     9421 atcgcctgct gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag
     9481 gttatctgca ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata
     9541 gttccggttt ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag
     9601 acggaaccgt gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc
     9661 ctggacaggg gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga
     9721 tgatttataa aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta
     9781 acgtaggaaa atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg
     9841 atttcccggg tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt
     9901 ttatctgtat tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg
     9961 cggaacagtc gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc
    10021 gcgcgggcct gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac
    10081 cgaccttcct ggtggcaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt
    10141 tctactcgct tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat
    10201 taagtctgct ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag
    10261 tcgacgaaga cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag
    10321 aggaagacga cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga
    10381 acgtaggtaa gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg
    10441 acatgcctgg cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt
    10501 atgtgctcat tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga
    10561 aattctccgt aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga
    10621 ttgatgcgcg cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata
    10681 gtgggcgctc acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag
    10741 agcaggtgaa agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact
    10801 ttatctctgc cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt
    10861 atgacagctc cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg
    10921 ttgaagatca ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg
    10981 ctggtggtta taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg
    11041 attcctacaa gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc
    11101 cgattcgtat tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga
    11161 cgccaaccca gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat
    11221 tatccgtaag tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag
    11281 gaattcatat ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc
    11341 atgcgcgttg cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc
    11401 atcaaccgtt gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc
    11461 atggcttgat atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg
    11521 atttttttac cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg
    11581 tggttagcat ttcacccgct ttaacttgct tcactttttt cagtacattg ccgtcagtag
    11641 cagtagtaac gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg
    11701 ttactgcatt accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta
    11761 aacgatattc cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt
    11821 gctgattcag ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt
    11881 ccagcgcaaa gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc
    11941 ggagctgcgg atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgcgtacggt
    12001 tggcgagata ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca
    12061 gtaactcttg ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca
    12121 gatcggcaac aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg
    12181 gaatgcggct ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt
    12241 ccagcgaacc gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca
    12301 gttcaatggc gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga
    12361 tgatcaccgg tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag
    12421 caccggtttt tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt
    12481 attgctgatc gaacaaacct tcagcctgca acttcgcttt gagctgttcg tacttctgtt
    12541 gcagcagccc ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg
    12601 gctcgtagag cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg
    12661 tgacccggcg gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga
    12721 gtgtaaagta ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc
    12781 aaacctgtcc catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg
    12841 taaaaattgc aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag
    12901 gttattcagt cgatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg
    12961 tagatgcaat cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc
    13021 gagatattgc ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc
    13081 gttcctgctc attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa
    13141 actattcgtc tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc
    13201 ctggctattg ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt
    13261 gaacgccagg cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat
    13321 ccgcagactg ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac
    13381 ggttttgcgg gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt
    13441 cgtgacgtgc gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa
    13501 gagcgtctgg tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac
    13561 gaaaaacgcg ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc
    13621 accgtgaaag acttccagaa agcggaacgt aaaccgaacg cctgtaaaga cgagcaaggc
    13681 cgtctgcgtg ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg
    13741 ctggttgccg ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc
    13801 gttttgcagc gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc
    13861 aacgtggcaa cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa
    13921 gtcggtatcg gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg
    13981 cagatcactg ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc
    14041 gcagatggtg gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc
    14101 gcggtgatgg taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc
    14161 taccagggcc gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa
    14221 ggttcctctg accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt
    14281 atcgaaggtc gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc
    14341 ggcctgcgct cctgtatggg gctgaccggc tgtggtacta tcgacgaact gcgtactaaa
    14401 gcggagtttg tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc
    14461 attactaaag agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgacttcatg
    14521 tcgggcgatt tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca
    14581 ttcataagca tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc
    14641 gccgcgtgcg tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac
    14701 aaattcgtga cttcaatcca agcggcatta ttctttccgg cggcccggaa agtactactg
    14761 aagaaaacag tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg
    14821 tttgctatgg catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg
    14881 aacgtgaatt tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta
    14941 tcgaagatgc gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg
    15001 ataaagttac cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt
    15061 ttgccattat ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga
    15121 ctcacacccg ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg
    15181 aagccctgtg gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg
    15241 taggcgacga taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa
    15301 tgctgctgca ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc
    15361 tgcgtctcaa cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca
    15421 ttgttcacgt accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg
    15481 aagcaaaacg taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgcaga
    15541 aactggaaga cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg
    15601 cggcttctgc aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc
    15661 cgaaagagat gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc
    15721 gtaagattgg tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg
    15781 gaccaggcct tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc
    15841 gccgtgctga cgccatcttc attgaagaac tgcgtaaaac ggacctgtac gacaaagtca
    15901 gccaggcgtt caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta
    15961 agtatgactg ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact
    16021 gggcgcatct gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga
    16081 acggtatttc ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg
    16141 aatgattttg atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt
    16201 tatcttcaag aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga
    16261 tttgttctct tctgctttgc ccacgctttt catcagatcg cgaccgtctt cagtcatatt
    16321 tctgtttgcg tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc
    16381 ctgatcgata aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt
    16441 ttgtttctga atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc
    16501 cgcagtggct tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc
    16561 cgctcttttg ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc
    16621 cctgttgcgc tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg
    16681 ttccgcttct gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct
    16741 taaggaggct aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct
    16801 cttttcagga catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac
    16861 aacggctaaa cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt
    16921 ggtgccttcc ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga
    16981 acccgctgcc gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct
    17041 gccagaaccg acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa
    17101 ggcttgtgcg ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga
    17161 aataatatta acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc
    17221 ggcggcatga tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc
    17281 cacaggcaga agacattttt taaatttcat cactattcct tgttatacac attacgaaaa
    17341 ataagacatt taagatcatc aaaagaggaa agtaaatgta ttgcgtataa agggatagat
    17401 taaatttatt aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat
    17461 attaatttta tatttatttc gcagccaaac aggataaaca tcagtcagga caaagacgta
    17521 aaatttaaga tgaaagaata tctgccgctt tgataatccc aaagagcaaa agcatccgac
    17581 agcgaagtgc cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg
    17641 attcgacgga gagtgtggat tgctgagctt cttttttgct ctgatggtgc agccaggcac
    17701 cgacggacga gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac
    17761 gggtcatgcg actgttaaat cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag
    17821 gttccttgaa gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa
    17881 atggtcaatt cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa
    17941 gttaatttaa ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat
    18001 aattcattaa tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact
    18061 tagaatcaat gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg
    18121 tctgaataca cctccttcag gatgtggggg attctgctga gcatctatga aactgaatg
//
LOCUS       DADMFL010000076        17684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.076, whole genome shotgun sequence.
ACCESSION   DADMFL010000076 DADMFL010000000
VERSION     DADMFL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.076"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNZ27_002674"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNZ27_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY8664953.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="JNZ27_002675"
     CDS             962..1303
                     /locus_tag="JNZ27_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY8664954.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="JNZ27_002676"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="JNZ27_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="JNZ27_002677"
     CDS             complement(1901..2611)
                     /locus_tag="JNZ27_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY8664955.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="JNZ27_002678"
     CDS             2719..3024
                     /locus_tag="JNZ27_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY8664956.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..4331
                     /locus_tag="JNZ27_002679"
                     /pseudo
     CDS             3223..4331
                     /locus_tag="JNZ27_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            4365..4688
                     /locus_tag="JNZ27_002680"
     CDS             4365..4688
                     /locus_tag="JNZ27_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8664957.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(5281..6270)
                     /locus_tag="JNZ27_002681"
     CDS             complement(5281..6270)
                     /locus_tag="JNZ27_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664958.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6345..6854)
                     /locus_tag="JNZ27_002682"
     CDS             complement(6345..6854)
                     /locus_tag="JNZ27_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664959.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6956..7177)
                     /locus_tag="JNZ27_002683"
     CDS             complement(6956..7177)
                     /locus_tag="JNZ27_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664960.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRHLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7424..8269)
                     /locus_tag="JNZ27_002684"
     CDS             complement(7424..8269)
                     /locus_tag="JNZ27_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664961.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
     gene            8508..9593
                     /locus_tag="JNZ27_002685"
     CDS             8508..9593
                     /locus_tag="JNZ27_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8664962.1"
                     /translation="MTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQ
                     DDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSA
                     KFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEKMKT
                     TGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIH
                     PIPAYTSGFDGWDAPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDA
                     QARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSI
                     NILTSHRPSPLAKGNPSHSNLVQIEKV"
     gene            9604..10221
                     /gene="dmsB"
                     /locus_tag="JNZ27_002686"
     CDS             9604..10221
                     /gene="dmsB"
                     /locus_tag="JNZ27_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8664963.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10223..11077
                     /locus_tag="JNZ27_002687"
     CDS             10223..11077
                     /locus_tag="JNZ27_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY8664964.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11120..11734
                     /gene="dmsD"
                     /locus_tag="JNZ27_002688"
     CDS             11120..11734
                     /gene="dmsD"
                     /locus_tag="JNZ27_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY8664965.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            11893..13110
                     /gene="clcB"
                     /locus_tag="JNZ27_002689"
     CDS             11893..13110
                     /gene="clcB"
                     /locus_tag="JNZ27_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY8664966.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTGAPGGVFTPTLFIGLAIGMLYGRSLGLWFPDG
                     EEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEYQLLPGLLIACVIASVISRTLH
                     RDSIYRQHTAQHS"
     gene            complement(13063..13758)
                     /gene="bioD"
                     /locus_tag="JNZ27_002690"
     CDS             complement(13063..13758)
                     /gene="bioD"
                     /locus_tag="JNZ27_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8664967.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALGEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(13883..15103)
                     /gene="mlc"
                     /locus_tag="JNZ27_002691"
                     /gene_synonym="dgsA"
     CDS             complement(13883..15103)
                     /gene="mlc"
                     /locus_tag="JNZ27_002691"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY8664968.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15238..16131)
                     /locus_tag="JNZ27_002692"
     CDS             complement(15238..16131)
                     /locus_tag="JNZ27_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8664969.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16238..17491
                     /locus_tag="JNZ27_002693"
                     /pseudo
     CDS             16238..17491
                     /locus_tag="JNZ27_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         4225 a         4521 c         4446 g         4492 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtatatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa taggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcggtt
     3301 tttctttacc gttcaccctg cgcagtgcag cggcagcggt acaacaggcc agcgaaaaag
     3361 tgatctgggg ttcctgttcc gtcaactgtg gtagccgctg tgcacttcgt ctacatgtta
     3421 aagataatga agtgacctgg gtggaaactg acaataccgg cagcgatgag tacggcaacc
     3481 atcaggtacg cgcctgtttg cgcggtcgct ccatccgccg gcgtattaat catcccgatc
     3541 gcttgaatta cccaatgaaa cgcgtgggta aacgcggtga aggcaaattc gaacggatta
     3601 gctgggatga agccctggat actatcgcca gtagcctgaa aaaaaccgtc gaacagtatg
     3661 gcaatgaagc tgtatatatt cagtactctt cggggattgt tggcggcaat atgacccgct
     3721 cttcgccatc agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt tcactcaatc
     3781 agtatggctc ctacagcacc gcgcaaattt cctgtgccat gccctacacc tacggcagta
     3841 atgatggcaa cagcaccacg gatattgaaa acagcaagct ggtcgtgatg tttggtaaca
     3901 acccggcaga aacccgcatg agcggtggtg gcatcactta tcttcttgaa aaagcgcgcg
     3961 agaaatcgaa cgccaaaatg attgttatcg atccgcgata taccgatacg gctgccggtc
     4021 gtgaagacga atggctccct attcgcccgg gcaccgatgc cgcgctggtt gcgggtattg
     4081 cctgggtatt gattaacgaa aatctcgttg atcaaccttt tctcgataaa tactgcgtcg
     4141 gctatgacga aaaaacctta cccgcagatg cacccaaaaa tggtcactat aaagcctata
     4201 ttcttggtga aggtgacgat aaaacagcaa aaacgccgca gtgggcttcg caaattaccg
     4261 gtatcccggt agaccgtatc atcaaactgg cgcgtgaaat tggcacaaca aaacccgctt
     4321 atatctgcta gggctggggg ccgcaacgcc aggcaaacgg cgagctgact gcacgcgcta
     4381 ttgctatgtt acctattttg acgggcaatg tcggcatcag cggcggaaat agtggcgcgc
     4441 gtgaatcgac ctataccatt acgatagaac gcctaccggt gctggataat ccggtcaaaa
     4501 cgtcaatctc ctgcttcagc tggacggatg ctatcgatca tggcccgcaa atgacggcac
     4561 tccgcgacgg tgtccggggc aaagataaac tggatgtgcc cattaagttc atctggaact
     4621 acaaggactt gaagcggtta ctcgttagag tcacgagaga ccttagtagg cctgaggtcc
     4681 tgcattgagt caagaccttg tgtcattctt tgtgccagac ttgtgtcatt agacgggatg
     4741 tctagacggc tggtcaggac ccccggtggc ctctttgtca ggactcaggg tggcaccggg
     4801 gaacattggt gtcttaattg tattgaatga ggtctctaac ttttggtcca aaactcatcg
     4861 ttagctgctc gccagctcac agccgaaccc gaactgtaag cacgaccacc agcaccagct
     4921 tcagcaggaa gacgacaaga tagactttat gcaggtccat gcgccctcct gtgtctattg
     4981 taccactgca ctagataact agtacaaaat aaagtccaga ccgaaggccc agaccatagt
     5041 taattaatca cattgataat ccaccacctg aagtcctgcc aacattcagt ccagccttac
     5101 ccatgctagg agtcttaacc ggggtgtcgt tctgtgtctc caccactggt gccgcatcag
     5161 gctctacagg aagctccaca gcaggcgcag ggaccggagg tgatacggat gtaccaccac
     5221 cagcggagaa gcccttagcg atggaccgga ggcctttact aatgctctta cccattgctg
     5281 ttacgcttcg gtcttggtga acttcaggcg tttgactaca ggcaggactg cggttgcagc
     5341 gccagtgtcc agcttaactg tcgtagcagc ttcagcgaca acgaagacct taccgaggat
     5401 ttccaagtcg tgggactgag agtcgccatc acgtgaaagg acgaccgtaa tattctcacc
     5461 gtcgtgacgg aagcagccga agctcagggc cgtgaagcgt ttagccagca tgaacacacc
     5521 tttgtcctca ccgttgtaat acgcgatgtc ggtacccagc atatccacga tagcgtcttc
     5581 accagagaag ccctgtttag cagccagacg ggacagcgct ttcagttggg actcatgggc
     5641 gatgatgccg taatcagaca cttcagagcc gaaagaggac tcggtgttct caaacagagc
     5701 gtccatgata gcgtctgcct tagtggtcag gcttccagtc aggactggca gctctacggt
     5761 cttcagcagc ttggaggact ccagaacgtc aaccatgcga gcgttgatgg tacggaccac
     5821 attctcacag atgttcatag cgtcctgctg gagtgtgcca tgagtgaaac cagtggagga
     5881 cttggcgtcc cacagtttag tttgctccga gatgaggatt gcctgttcga taggttcgac
     5941 gacaatctcc tgaccagacg gaacggtcac gacttcaggt cgctcaccac cgcgtacagg
     6001 gacgaccaca gagcggtcag acagggtgtg ggcgatgatt ggggtaggca ggctattgcg
     6061 ggtaggcagg acttctacag ggaacggaat gttatcctga gcgtctttaa gtacaccgtt
     6121 cagcatagcc ttaaccgtct tagctaactg gagggcatag acctgtgggt cagcgttagt
     6181 tggcagacct tcgttaacct gaatgttgtc ggtgtcggtg atacgtactg gctcaatgcc
     6241 gaactgatag ctatctttaa tcatggtcat aataatcaat ctcctaaatt agttttagat
     6301 actgtgggtc ccgacatcag gtcaggacca cttgtgtgtc tgtcttagag gctgtccatc
     6361 cagttaccgc ctgactcaat gtcagcgaca atagcggcac gggagcgacc agcggaatgg
     6421 gtagcactgc ggaggttctc accataggtc cggcgctcag ctttgacctg ttcgcggcgc
     6481 tgtagctctt tctgccactc tgcatcggtc atgttctctt cacgcatacg ctcagcctga
     6541 atgcgctgct ctttagcgat acgctcagcc tcggctcgct tgttctgctg gcgacgttgg
     6601 taggcggtca tcaggtcatt gctgaagaag tttccaatgg tcacgccagc ttcagccaga
     6661 gcttccagta attccatgga ctcgcggctg gtggcaggca gtcctgacag gtagcccgga
     6721 atcagggtct taatagcgaa ggacagtggg taggactgac cgtccagact cacagtgttt
     6781 tcgtagagat tgatgtcggt gacatggagt ttccagtcat tgttgttgaa taacgcacta
     6841 gctttgctag acatagtaat tactcctctt gagagttgag actatcggta ccgcagacac
     6901 agttatgcct acgggtctcc ccgcatcggt catctcaaaa ggtttaaatt aggggttagt
     6961 gagtggcgtc cagcttagcg aacgctcggc gtaactgatt gttcaggtca cggtcgacca
     7021 gcaggtctat cagttcgtct cgactataga aaggacgctc agggtcctct acgcccatcc
     7081 ccagcattga caggaggtga cgggtaggca gaccacggta acggagagta gcctttccga
     7141 tgggtgtccg tgggtcgata tgttgtttag ttgtcattgt tagaagtcct cgttagacca
     7201 atcatccacg tcaaattcac tggtgatagg agcttggaag ttaggtagac cctctggtat
     7261 ctctagaggc agtcgctcgt cgactctcag tgccatctct tcgtgtatat gccgaagcag
     7321 agaagtcagt aagatagggt ccgcaggatg ctcagggccg tgtctctcaa tgaaacatat
     7381 ggttgtcgct attgactcca taatcagttc atcagtgagt cgcttagaac ccatagtcag
     7441 accccttgcc cgctgctgct ttctgggact ttgtagcctt accattcagg tgagaatcca
     7501 ccagacgacg gacaaccttg atattggatg tgcgcatgtc atgagcgata tccttccagt
     7561 tatccttcag ccagtcataa cccttgcgta gaatcttgat ggtaggctct ttgtcgttag
     7621 gctgtggctg tagacgatag tagccgttgg tctcaccttt cttattgaag atggctcgtg
     7681 gacgtccggt cgataggtct atatccgtag tgccaaactc agtaacacgc ttggtgtaga
     7741 atgggttagg acgacacagg gtcttcagat atccagtcag gacgctagct gtcagcatct
     7801 tagagctggt caacttaagg tccagagctt taataacctc ggtaggcgtg gcgcctgact
     7861 tggagtccag cttggagtcg aggacgatag caccgggcgc atagcagata gctttggtct
     7921 gtgcgacctg aagttgctgg gcctgaagtt cgaggacctc ttgctccttc tggcgaatat
     7981 aggctaggac cttacgacgc acagctttag actcacgcat cccgaccagt agaagctggt
     8041 cagtgttgag ggtatagctt ttaacgtcct gcccgaagct tacgaaaatt tcgtaggcta
     8101 cattttgacc gtccagctca tcttcaatac gggacacaaa gtcgttgtta cggacaggct
     8161 tctccccagc ctccacacga gccgggttga tgaccttagt caagaaatcg tagctactca
     8221 tagacacaag ctggtcaagg ttgatgacgc taacttcgtt ggtattcata aaggatgctc
     8281 ctttggtaaa gcactttctg tctccatgtg gagtccgcac ctattcaatg catccaaacg
     8341 gacccaccaa aaagtgctta acgaaagtga atagggttaa tgttgattag tgttggtctg
     8401 gagtgaccat gttctccatg tgcatataag gcgagaccgc atgtcaggac gagctgtaag
     8461 gaccccagat gtttacatgg acagatgcca tcgaccatgg tacgaaaatg accgcgaccc
     8521 gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg
     8581 ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg
     8641 atgacagcaa gtgcgagatg attgttggca ttgaccactt catgacggcc tcggctaagt
     8701 attgcgatat cctgttgcct gacctgatgc caacagagca agaagacctt atctctcatg
     8761 aatctgcagg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg
     8821 aacgcaaacc catctactgg atgctgagtg aagtcgccaa acgcttagga ccagacgttt
     8881 atcaaacctt tactgaaggt cgcagtcagc atgaatggat caaatatctc catgcgaaaa
     8941 cgaaggaacg taaccctgag atgcccgact acgagaagat gaaaacgacc gggatcttta
     9001 agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccacagg
     9061 caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga
     9121 ttgcagatac ctgggaattg aaaaaagatg aaattattca tcccattcct gcgtatacct
     9181 caggttttga tggctgggac gctcccctgc ggaaaaccta tccactgcag ttaacgggct
     9241 tccactacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct
     9301 gcccacaaga ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg
     9361 ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc
     9421 gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg
     9481 gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc ccttcaccgc
     9541 tagcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag
     9601 cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc
     9661 tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt
     9721 tatgaatatg ctggcggcga ctggcaggaa gataatggca tctggcacca gaacgtgttc
     9781 gcttattatc tctccatctc ctgtaaccat tgcgataacc ccgcatgtac aaaagtctgc
     9841 cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgatga agatgtttgt
     9901 atcggctgcc gctactgcca catggcttgt ccgtacggcg caccacagta caatgctgaa
     9961 aaagggcaca tgacgaagtg cgatggttgt tattcgcgcg tcgccgaggg gaaacaaccc
    10021 atatgcgtcg aatcctgccc gctgcgtgca cttgagttcg gtccaattga agagctgcgc
    10081 cagaagcacg gtactctggc tgccgtcgcg ccactgccgc gcgcgcattt cacaaaaccc
    10141 aatatcgtta tcaaacctaa cgccaacagc cgcccgaccg gcgataccac tggttatctg
    10201 gctaatccgg aggaagtgta aaatgggaaa tggatggcat gaatggccac tggttatctt
    10261 taccgtattg ggtcaatgcg tagtaggcgc gctgattgtt agcggaattg gctggttcgc
    10321 cgccaaaaat gacgcagacc gtcagcacat tgttcgtggc atgtttttcc tctggctgtt
    10381 gatgggcatc gggtttattg cctctgtcat gcacctggga tcgccactgc gagcgtttaa
    10441 ctcgctgaat cgaatcggag cttctggtct gagtaacgag attgctgccg gttccatatt
    10501 ttttgccgtt ggcggactat ggtggctggt ggcggtcatc ggtaaaatgc cgcaagcgtt
    10561 ggggaaactc tggttacttg tcagcatggc gctcggcgtc attttcgtct ggatgatgac
    10621 ctgcgtgtat caaatcgaca ccgtgccaac ctggcataac ggttacacta cgctggcttt
    10681 cttcctgacg gtattgttga gcggtccaat acttgctgcc gcaattctgc gggcagcccg
    10741 cgttacattt aataccacac catttgccat cattagcgtt ctggcgctga tcgcttgtgc
    10801 cgaggtgatt gtgctgcaag gtttgtctct ggcgtctatt cactcatccg tgcaacaagc
    10861 cagcgcactg gtaccagatt acgcctcctt acaggtctgg cgtgtggtat tgttatgcgc
    10921 aggtcttgga tgctggcttt gtccgctcat tcgtcgccgt gaacctcacg ttgccggact
    10981 tgttctgggg ctaatcctca tacttgttgg tgagatgatc gggcgtgtgc tcttttatgg
    11041 cctgcacatg acagtaggaa tggctattgc aggttaacaa attggtgcgg ggcaacccgc
    11101 acatttcagg atgttaggaa tgacccattt ttcacagcaa gataattttt ctgtcgcggc
    11161 gcgcgtgttg ggagcgctgt tttattacgc tccagagagt gcggaagccg cacctctggt
    11221 tgcggtactc accagtgacg gctgggaaac tcagtggcct ctaccagaag cgtcattagc
    11281 gccgctggtg acggcttttc agacacagtg tgaagagact cacgcccagg cctggcagcg
    11341 tttgtttgtc ggcccgtggg cactgccgtc tccgccgtgg gggtcggtct ggctggatcg
    11401 cgaatctgtg ctgtttggcg attcaacatt ggcacttcgt cagtggatgc gcgagaaagg
    11461 cattcagttt gaaatgaagc aaaacgaacc ggaagatcat tttggttcac tgctgttgat
    11521 ggctgcgtgg ttggcggaga atggtcgcca gacagaatgc gaagaattgc tggcatggca
    11581 cctttttccg tggtcaacac gttttcttga tgtttttatc gaaaaagcag aacacccttt
    11641 ctaccgtgca ctgggtgaac tggctcgctt aacgctggcg caatggcaat cacaactgtt
    11701 gattcctgtc gcggttaaac cactctttcg ataggatcac cgtaatattg ccggatggtg
    11761 acgattacgc gcgattcggc aataatacgt tacaatgtca cgttttttta tctcttaaag
    11821 cacgcactgc ttttgcggtt ggcctctttt gccgcaaaat agtcgcccgt gtttcattgc
    11881 ccatttctgc tcatgcatca tctacacatc tatccggatc tgcgcactat gttccgccgt
    11941 ctgcttatcg caacagtcgt cggtattctc gctgtctttg ccgttgccgg gtttcgtcat
    12001 gcgatgctgc tactggagtg gttgttcctc aataatgact ccggcagtct ggtcaatgca
    12061 gcgacaaacc tttccccctg gcgacggctg ctaactccgg cgctcggcgg actggcggcg
    12121 ggtttgttgc tgatgggctg gcagaaattt acccaacaac gccctcatgc gccgaccgat
    12181 tacatggaag cgttgcaaac cgatggacag ttcgattacg cagcaagcct ggttaaatcg
    12241 cttgcctcgc tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg tgcgatgatt
    12301 cttttagctg cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg ccaggaatgg
    12361 aaattatgga tcgcctgtgg ggccgcggcg ggaatggctg cggcctatcg tgccccgctt
    12421 gctggcagtt tatttatagc cgaagtgctg tttggcacta tgatgttggc ctctctcggc
    12481 ccggtgatta tttccgccgt cgtggcattg ctggttagca atctgattaa tcatagcgac
    12541 gcgttactct acaacgtaca actctcagtg acggttcagg ctcgtgacta tgcgctgatt
    12601 atcagtacag gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt aatgaacgcc
    12661 tgtcatcgtg gatttgtgag tctcaaactt gcgccgccct ggcaactggc actaggcggg
    12721 ttgatcgtgg gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg ctatagcacc
    12781 gtacaatcct ttttaaccgg cgcacccggt ggtgtcttta caccgacgct atttatcggt
    12841 cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa
    12901 attacgcttt tactcggatt gaccgggatg gcgacactgt tggcggcaac cacgcacgcg
    12961 ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt
    13021 ttattgattg cctgcgtaat tgcgtcggta atttcgcgga cgttacaccg tgactctatc
    13081 taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt cacgctgttc
    13141 ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac
    13201 atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat
    13261 gagcggcagc ccgtcgttgg cgatcgcctg agctgttagc agtgcatggt taatgcaacc
    13321 ttcctgaata ccgacaacca tcaacaccgg cagttgttcc tgcactaccc attcagagag
    13381 tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc
    13441 gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact
    13501 gtgcgccacg ctactttctt cttcgcctaa cgcgatagga ttaactgctt cataaggcag
    13561 ttcgatggtt gaaacactct gcaacaccaa ggcatcttta ttacgcagcc cttcgggtgt
    13621 ctctttgctc cccttcgcta cgggtttata tcccgcaacc gtttttccct gggaggctaa
    13681 cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat
    13741 aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata aactaggaaa
    13801 ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt
    13861 ttggtagaac agttaaaaaa tgttaaccct gcaacagacg aatcaacaaa gaaccgttat
    13921 acatcgcgtc ttttaccagt gcagcgcctg ccatcgtgcc ctggttagaa aactgagtac
    13981 tctcaacgct aatgtgctga ctatacgcag gaagggcctg ctgacggatg ctgtctgaga
    14041 tgaccgggaa gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg
    14101 ggttaaataa attcaccatg atggcaagaa tgcgccctac atgcgcgccc accccgataa
    14161 tgatgtcttt tgccagtaga tcgccgcgca atgccgcctg acacaatgag tccacggtta
    14221 acggttgccc atgtaacatc gagctcatgg attgattgag acgcagctgt gccagctcaa
    14281 gaatactgtc cacactggcg atggtttcga ggcagccgtg gttcccgcaa taacagcgtt
    14341 taccatatgg gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta
    14401 gcagatgacc atcggtaatg acgcccaccc ccacgttgtg atcgataacc acctgaatca
    14461 catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctgatat
    14521 catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct
    14581 cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc
    14641 ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa
    14701 actgatcgat atgggaaatg atacgatcca gcaatggcaa gtcatctttt aacgccagtt
    14761 cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctccc
    14821 cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agccccaccg
    14881 ccggacggcc acggttcccc gcttctttga tttccagctc tcgcaccagg tgtgcttcga
    14941 gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat
    15001 cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct
    15061 gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt
    15121 ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt
    15181 tttacttagg catgtgatta acagcacatt tttcgagctt tttcgctgaa atttccctca
    15241 cctgagagca ttcagcagat gaatacgaaa gttacgcgca gctggacttt gttcatgatg
    15301 tttcggccag accaaccaca tttcagaaac cgcatcctct tcagcaatcg gcacccagcg
    15361 catttcgttg agctgaacac gtttaaatga cgcaggcaaa attgaaacac ccagaccggc
    15421 ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa
    15481 atggtaacgt cgcatcagtc cgagaatatc gtcatacagc ccagtcccga cgtgcggatc
    15541 aaaaaagaca aagggttctt tcgccagttc agccagtgtt acactcggat tatttgccag
    15601 gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag
    15661 cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg
    15721 agcgatttgc tcgcgagtgt tcatttcgcg ggtttgtaaa tggacatcag gataatcacg
    15781 gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc
    15841 aatacgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc
    15901 caccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa
    15961 tacactgcga ttagttcgtg ccagcagtcg ggcaccgatt tgttgctcca gtgcctgaat
    16021 ctgctgactt agcggcggtt gcgaaatatt caggcgggca gccgcgcgcc caaaatgcag
    16081 ctcttccgca acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac
    16141 atcttatttg agattattaa tatattagac agaacaattc gattttccta ccctatgtat
    16201 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
    16261 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
    16321 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
    16381 ctttgcactt ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
    16441 aacgcccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctatcggttt
    16501 gctatttact ggcccgctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
    16561 actgttggcc tccatttgta cgttactttc gacaatgatg accagctggc acggcatttt
    16621 gattatgcgc gccttgattg ggctttcgtt aagtggcgtg gcagctgttg gcatgactta
    16681 tcttagcgag gaaatccacc ccagtttcgt ggccttttcg atggggttgt atatcagcgg
    16741 caactcaatt ggcggcatga gcggacgctt aattagtggt gtcttcacgg actttttcaa
    16801 ctggcgaatt gctctggcgg caatcggttg tttcgcgctg gcctcggcgt tgatgttctg
    16861 gaaaatcctc cctgaatcac gccatttttg cccgacttcg ctgcgcccta agacgttatt
    16921 tatcaacttt cgtctgcact ggcgtgaccg gggattaccg ttattgttcg cagaaggctt
    16981 tttgctgatg gggtcgttcg tcacgctgtt taattacatc ggctatcggt tgatgctttc
    17041 cccctagcat gtcagtcagg ccgtggttgg cttattatcg ctggcttatt tgaccggtac
    17101 atggagctca cccaaagccg gaaccatgac cacccgctat gggcgcggtc cggtgatgtt
    17161 gttttcgacg ggagttatgc cgtttggttt actgatgacc ttatttagct cgctgtggct
    17221 gatctttgcc ggaatgttac tcttctcagc cggattcttc gcagcccact cagtagccag
    17281 cagctggatc ggcccccgcg caaaacgcgc taaaggccag gcctcctcgc tgtatctgtt
    17341 cagttactat ctggggtcga gtattgccgg gacgctgggt ggtgttttct ggcataacta
    17401 tggctggaac ggcgtcggcg catttattgc tctgatgctg gtcattgctc tgctggtcgg
    17461 ggcgcgtttg catcgtcgtc tgcacgcctg aaaaataagt ccggactgcg gtaaataccc
    17521 gtccggactt attgcctgct caaaccaacg ttaatagcca tcctaaaata gacgaagcgc
    17581 cagccaattc ccgcagcgca tctagcgtca tcaggattat aagtacccaa ataaacggat
    17641 tcattttgct gtgtgtcatt tattactgat gcgcagttat tcta
//
LOCUS       DADMFL010000077        17459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.077, whole genome shotgun sequence.
ACCESSION   DADMFL010000077 DADMFL010000000
VERSION     DADMFL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.077"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            564..1334
                     /locus_tag="JNZ27_002694"
     CDS             564..1334
                     /locus_tag="JNZ27_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY8664970.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JNZ27_002695"
     CDS             1325..1798
                     /locus_tag="JNZ27_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY8664971.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JNZ27_002696"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JNZ27_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY8664972.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JNZ27_002697"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JNZ27_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY8664973.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JNZ27_002698"
     CDS             3233..3727
                     /locus_tag="JNZ27_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY8664974.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JNZ27_002699"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JNZ27_002699"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY8664975.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JNZ27_002700"
     CDS             complement(6410..7432)
                     /locus_tag="JNZ27_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY8664976.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="JNZ27_002701"
     CDS             7571..8485
                     /locus_tag="JNZ27_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8664977.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="JNZ27_002702"
     CDS             8700..10061
                     /locus_tag="JNZ27_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664978.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="JNZ27_002703"
                     /pseudo
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="JNZ27_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="JNZ27_002704"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="JNZ27_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY8664979.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="JNZ27_002705"
     CDS             12611..13900
                     /locus_tag="JNZ27_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY8664980.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="JNZ27_002706"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="JNZ27_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY8664981.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16215
                     /gene="hpaD"
                     /locus_tag="JNZ27_002707"
                     /pseudo
     CDS             15365..16215
                     /gene="hpaD"
                     /locus_tag="JNZ27_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
     gene            16225..16606
                     /locus_tag="JNZ27_002708"
                     /pseudo
     CDS             16225..16606
                     /locus_tag="JNZ27_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
     gene            16674..>17459
                     /gene="hpaH"
                     /locus_tag="JNZ27_002709"
     CDS             16674..>17459
                     /gene="hpaH"
                     /locus_tag="JNZ27_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY8664982.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4291 a         4626 c         4377 g         4165 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtaataa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgcgg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg catagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgagccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccacaaga atccacatcg cctagctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaatcat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattaa atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg cctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggtgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccaacc gcttgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gagcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcaacag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaagttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaactgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagccagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaccc ggagttgggg
    15661 cagctaattg ctgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcatgg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag ggctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaggtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgctggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatt
//
LOCUS       DADMFL010000078        17407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.079, whole genome shotgun sequence.
ACCESSION   DADMFL010000078 DADMFL010000000
VERSION     DADMFL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.079"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..537)
                     /locus_tag="JNZ27_002710"
                     /pseudo
     CDS             complement(1..537)
                     /locus_tag="JNZ27_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            545..1045
                     /gene="tnpA"
                     /locus_tag="JNZ27_002711"
     CDS             545..1045
                     /gene="tnpA"
                     /locus_tag="JNZ27_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY8664983.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1084..1431)
                     /gene="grxD"
                     /locus_tag="JNZ27_002712"
     CDS             complement(1084..1431)
                     /gene="grxD"
                     /locus_tag="JNZ27_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY8664984.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1766..2593
                     /locus_tag="JNZ27_002713"
     CDS             1766..2593
                     /locus_tag="JNZ27_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY8664985.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2721..3302
                     /gene="sodB"
                     /locus_tag="JNZ27_002714"
     CDS             2721..3302
                     /gene="sodB"
                     /locus_tag="JNZ27_002714"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY8664986.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3448..4617)
                     /locus_tag="JNZ27_002715"
     CDS             complement(3448..4617)
                     /locus_tag="JNZ27_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8664987.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATRLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4783..4872)
                     /gene="ynhF"
                     /locus_tag="JNZ27_002716"
     CDS             complement(4783..4872)
                     /gene="ynhF"
                     /locus_tag="JNZ27_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY8664988.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5171..6196
                     /gene="purR"
                     /locus_tag="JNZ27_002717"
     CDS             5171..6196
                     /gene="purR"
                     /locus_tag="JNZ27_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY8664989.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6193..7125)
                     /locus_tag="JNZ27_002718"
     CDS             complement(6193..7125)
                     /locus_tag="JNZ27_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8664990.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7238..8449
                     /locus_tag="JNZ27_002719"
     CDS             7238..8449
                     /locus_tag="JNZ27_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY8664991.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8740..9888
                     /gene="cfa"
                     /locus_tag="JNZ27_002720"
     CDS             8740..9888
                     /gene="cfa"
                     /locus_tag="JNZ27_002720"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY8664992.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9928..10569)
                     /gene="ribE"
                     /locus_tag="JNZ27_002721"
     CDS             complement(9928..10569)
                     /gene="ribE"
                     /locus_tag="JNZ27_002721"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY8664993.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10784..12157
                     /gene="mdtK"
                     /locus_tag="JNZ27_002722"
     CDS             10784..12157
                     /gene="mdtK"
                     /locus_tag="JNZ27_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY8664994.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12198..13454)
                     /locus_tag="JNZ27_002723"
     CDS             complement(12198..13454)
                     /locus_tag="JNZ27_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664995.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13762..13838
                     /locus_tag="JNZ27_002724"
     tRNA            13762..13838
                     /locus_tag="JNZ27_002724"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13796..13798,aa:Val,seq:gac)
     gene            13843..13919
                     /locus_tag="JNZ27_002725"
     tRNA            13843..13919
                     /locus_tag="JNZ27_002725"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13877..13879,aa:Val,seq:gac)
     gene            14027..14332
                     /locus_tag="JNZ27_002726"
     CDS             14027..14332
                     /locus_tag="JNZ27_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY8664996.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14458..16062
                     /locus_tag="JNZ27_002727"
     CDS             14458..16062
                     /locus_tag="JNZ27_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY8664997.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(16074..16886)
                     /locus_tag="JNZ27_002728"
     CDS             complement(16074..16886)
                     /locus_tag="JNZ27_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8664998.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16890..17407)
                     /locus_tag="JNZ27_002729"
                     /pseudo
     CDS             complement(16890..17407)
                     /locus_tag="JNZ27_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
BASE COUNT         4310 a         4492 c         4136 g         4469 t
ORIGIN      
        1 cattgatgct gaagggtgaa ccggagttga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca actcccgtga cctgacggct
      121 gttccggtag cgcatccagc caagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcagc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggcgaat ctttaagcca ttgcgtttca gtggcgtttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgaagcgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acgctcctgt taaccaccgg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacacgaca gaaaaactac gcacttactt ttcaaatgta tgtgctgatt ttgcagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaact atcctcccaa
      781 actggcgata tccagtctgg taaacagcct caaaggcata tcgaataggt tactgcgacg
      841 agatcgacca gatattgcag tcaggtatta ctacaaaggc gttttgtgga gtcctggcta
      901 ttttgccagt agctgcgaag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca
      961 gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag gacgaggttt
     1021 tacggcgcac cggataaagg actgacggat gcccgttcgc atccgtcagt attgcaggac
     1081 ggattattcc gcgtccggct cttcagactt gtatttagcg gcagtttctt tgatcagctg
     1141 ctgcagttcg ccacgctgat acatttcgat cacgatatca caaccgccga ccagctcgcc
     1201 gtcaacccac agttgcggga aggtcggcca gttagcatat ttcggcagtt ccgcacgaat
     1261 gtccggattc tgcagaatat caacataggc aaaacgttcg ccacatgcgg caagcgcctg
     1321 gactgcctgg gcagagaaac cgcagctcgg cagtttcggt gaacctttca tgtacagcag
     1381 gatcgggttt tcagcaatct ggcgttggat tttttcgata gtggtgctca ttgtcttgct
     1441 tcctcaaact tctttacggc agtaatctga cattgtagcg ggtcagtgcg gcatcggaaa
     1501 ataacatttt catcacgctt ttgctatttt atccctttgc tctatttgtt gcatttcaaa
     1561 tattcgtttc ttctctgatg cgcggcaaac tggcgtattt ataaccattt ttagctttca
     1621 ctgctgcatt tttttgcgct cgccaacgaa acgtattttt taacaataaa agctattaac
     1681 tttctcttct tctatgcatt agaatcatca agttttgtaa atcagacgca ggcatgatag
     1741 acctgcctta acagagggac gctcagtggc gcggataaac cgtatttcga tcacgctctg
     1801 tgctttactt tttaccaccc tgcctttaac gcctatggcc catgcttcaa agcaagccag
     1861 ggagagttct gctaccgctc atatcaccaa aaaagcagat aaaaagaaaa gcacggcaac
     1921 caccaaaaag acccagaaag ccagccagaa aacagcgaaa aaagccgcca gtaaaagtac
     1981 gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc
     2041 caaaactcgc agcaaacacg ccgtcaataa aacggcctca gccagcttta ccgaaaagtg
     2101 taccaaacgt aagggctata aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac
     2161 ccttgccgac gcgcacaaag cgaaggtgca aaaagccacg aaagtggcaa tgaataaact
     2221 gatgcagcaa attggtaagc catatcgttg gggcggcagc tcaccgcgta ccggttttga
     2281 ttgcagcggc ctggtttatt acgcttataa agatttggtg aaaattcgta ttccgcgtac
     2341 ggcgaatgaa atgtatcacc tgcgtgatgc aggacctatc gaacgtagtg agctgaaaaa
     2401 cggcgacctg gtctttttcc gtactcaggg acgcggtaca gccgatcatg tcggcgtgta
     2461 tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt caggaaattc aaatcacttc
     2521 tctcagtgaa gactactggc agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa
     2581 aacacttcgc taaaacttta ccctgttgtt acggcaacag ggtaagttca tcttttgtct
     2641 caccttttaa tttgctaccc tatccatacg cacaataagg ctattgtacg tatgcaaatt
     2701 aataataaag gagagtagca atgtcattcg aattacctgc actaccatat gctaaagatg
     2761 ctctggcacc gcacatttct gcggaaacta tcgagtatca ctacggcaag catcatcaga
     2821 cttatgtcac taacctgaac aacctgatta aaggtaccgc gtttgaaggt aaatcactgg
     2881 aagagattat tcgcagctct gaaggtggcg tattcaacaa cgcagctcag gtctggaacc
     2941 atactttcta ctggaactgc ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg
     3001 ctgaagctat cgccgcatct tttggcagct ttgccgattt caaagcgcag tttactgatg
     3061 cagcgatcaa aaactttggt tctggctgga cctggctggt gaaaaacagc gatggcaaac
     3121 tggctatcgt ttcaacctct aacgcgggta ctccgctgac caccgatgcg actccgctgc
     3181 tgaccgttga tgtctgggaa cacgcttatt acatcgacta tcgcaatgca cgtcctggct
     3241 atctggagca cttctgggcg ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat
     3301 aataactgat ggcaaatgca gcattgcctg atgcgcttcg cttaccatgc caacgcggat
     3361 gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga
     3421 aggttatcct tctgcgtttt tgtttaatta gctgttagca acgcaaactg tttcaggttg
     3481 ttttctggct gacataaaca ccagcaataa tgccagtccc gcgacaatcg cccccgtcac
     3541 cggcacaaag ctgtatccca gcccagcgga aattaccgca ccaccagcag ctgctcccag
     3601 cgcatttcca agattaaagg caccaatatt gactgatgaa gacagacctg gcgcttcact
     3661 ggcgacatgc atcacgcgca tctgtaacgg aggtacaacc gcaaaggttg ctgcgcccca
     3721 caccaccatg ctaatagctg cgccgaactc attgcgggcc aggaacggga ttgccagcat
     3781 aatcaccatc agcagcaaca aaaagccttt caacgtgccg ttaactgaac gatctgccag
     3841 tttgccgccg agatagttac cgatagagaa tccgacacca atcagcacca gcattgccgt
     3901 gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc ggagagatat aggtgtagag
     3961 ggtaaacatt gcaccagctc ccagtaccgt cgtcagcaat gcagacagca cctgcggacg
     4021 cattaatacc gccagctctt ttttcacttc aggtcgtgcc cctgtaccac ctttaggtaa
     4081 tgagaagaac agacttacca ttgaaatcac tcccagcccc gccgttgcca gaaatgacat
     4141 ccgccagccg atggtttcac ccaacctggt cgccgccggc acgccaccga tatttgccag
     4201 ggttaacccc ataaacatag tggcaactgc gctggcctgt ttatgttttg gcaccacgct
     4261 tgcggccacg actgaaccca aaccaaaaaa tgctccgtga ttcaggctgg tcaaaatacg
     4321 tgaaagcatc agggtcatat aatccggcgc gatggcggaa agaacgttgc ctagcgtgaa
     4381 aattgccatc aggaaaatca acgcactgcg gcgggcacga tgagaaagta gaagcgtcat
     4441 cagcggcgcg cccaccatta cgccaactgc ataggcactg attaacattc cggcagcggg
     4501 aatcgagaca tccacaccgc gcgcaatgac gggcaacaag cccattggcg agaactccgt
     4561 tgtcccgata ccaaacgcgc caatcgccag cgccagcaac ggatagttaa ttttcatgcc
     4621 ttatctccac ctcttcgcgt cattacgcga tattcattaa agtggcgaaa gcatgacagc
     4681 aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt ttgccatttt gctaacaaac
     4741 aggaaggaga tgcgagggag aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt
     4801 caaacccacg gctacgatca aaccgaggac gataatcgtt gttaccagtg aaaatttaag
     4861 gtcggtgctc atcaagtttt ctcctttttt attaccacac aaaaagtgat attacgcatt
     4921 tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt tttaccactt
     4981 ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta ctggccattg
     5041 accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt gttgtgcgta
     5101 aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt agggtctgga
     5161 gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt ccactacaac
     5221 tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg
     5281 ggcagcgatt aaagaattac actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa
     5341 ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt ttgccgagat
     5401 cattgaagca gttgaaaaaa attgcttcca gaaaggttac accctgattc tgggtaatgc
     5461 gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga
     5521 tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc tggaagagta
     5581 tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact tcaccgatgc
     5641 ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga ttgaacgcgg
     5701 tcaccgcgaa atcggcgtca tccccggccc gctggaacgt aacaccggcg caggccgcct
     5761 tgccggtttt atgaaggcga tggaagaagc gatgatcaag gtgccggaaa gctggattgt
     5821 gcagggtgac tttgaacctg aatccggtta tcgcgccatg cagcaaatcc tgtcgcagcc
     5881 gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc
     5941 tgctgatgaa atgggcctgc gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt
     6001 gcgcaacgcg cgctatttta cgccggcgct gaccacgatc catcagccaa aagattcgct
     6061 gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca
     6121 gtccattgaa gtgcatccgc gtttgattga acgccgctcc gtggctgacg gcccgttccg
     6181 cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca gccactcctt
     6241 attcagcgtc tcactatcgc cgagatactc aagcagccag gttaacgcag gcgacatatc
     6301 attttgctgc catgtcagac aacatgccga atccgggaag gggttttcca gttcaagtgc
     6361 tacccacttc ccctcattaa gccacggttt ggcgaaatgt gttggcacca tccctatgca
     6421 taatcctgcc gagatacagg ttgccgatga ttcccagtca ggtacgacaa gtcttttttg
     6481 gttatccagc aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa
     6541 cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg ccagcgggtg
     6601 gtgactggca acaacgcaac tccagcttag catccccata tcccggaagg cataacgacc
     6661 gcctaccgga atcgcgcgtg ttgcgccaat tgccagttcc acgcgcccgt cggaaagcgc
     6721 atcccagaca ccgttgaaca cttcctgaaa gacaagaagt tcaacatcat caaaatggcg
     6781 ataaaaatca acgatcatct gccgtgtacg ttctggcctg acaatattat ccactgcgat
     6841 agctaactga ccgcgccagc cgttcgctat ctgctgacat tgctggcggg tgatctgcat
     6901 ttttttgaca acagagcgcc cttctttgag aaaccacgct ccagcagcgg tcagctccac
     6961 atcacggtgc cgtcgttcaa agagcggtac cgccagccac tcttccagct gacgcacggt
     7021 atagctgacc gcagaaggaa cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt
     7081 acgcgctacc gcatcaacaa cttcgagtga atattctgac cacatagtca gcctgcaaaa
     7141 tttttgaaac cagtcatcaa atattaccgt ttcacaacac taatttcact ccctacactt
     7201 tgcggcggtg tttaattgag agatttagag aatatacatg caacctggga aaagattttt
     7261 agtctggctg gcgggtttga gcgtactcgg ttttctggca accgatatgt atctgcctgc
     7321 tttcgccgcc atacaggccg acctgcaaac gcctgcgtct gctgtcagtg ccagccttag
     7381 tctgttcctt gccggttttg ccgcagccca gcttctgtgg gggccactct ccgaccgtta
     7441 tggtcgtaaa ccggtgttat taatcggcct gacaattttt gcgttaggta gtctggggat
     7501 gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt
     7561 ctgcgccgcg gcggttatct ggcaagcatt agtgacagat tattatcctt cacagaaagt
     7621 caaccgtatt tttgcgacca tcatgccgct ggtgggtctg tctccggcac tggctcccct
     7681 gttaggaagc tggctgctgg tccatttttc ctggcaggcg attttcgcca ccctgtttgc
     7741 cattaccgtg gtgctgattc tgcctatttt ctggctcaaa cccacgacga aggcccgtaa
     7801 caatagtcag gatggtctga cctttaccga cctgctacgt tctaaaacct atcgcggcaa
     7861 cgtgctgata tacgcagcct gttcagccag tttttttgca tggctgaccg gttcaccgtt
     7921 catccttagt gaaatgggct acagcccggc agttattggt ttaagttatg tcccgcaaac
     7981 tatcgcgttt ctgattggtg gttatggctg tcgcaccgca ctgcagaaat ggcaaggcaa
     8041 gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc
     8101 gggcttcatt agccatgtgt cgctggtcga aatcctgatc ccattctgtg tgatggcgat
     8161 tgccaatggc gcgatctacc ctattgttgt cgcccaggcg ctgcgtccct tcccacatgc
     8221 aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg ggtctgtgct tcctcgcaag
     8281 tctggtagtt tcctggctta ttagtatcag cacgccattg ctcaccacca ccagcgtgat
     8341 gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg caacgttgtg aagaagttga
     8401 ctgcccgaat catggcaatg ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat
     8461 atacttatac ttaggctgct aacaaaattt tgttgtatga ttgaaattag cggcctatac
     8521 taatttcgag ttgttaaagc tacgataaat attatgtttt tacggggaca ggatcgttcc
     8581 cgactcacta tggatggtca tttcggcaag ggttcctcct ttccctctgt tctacgtcgg
     8641 attatagact cgcggttttt tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct
     8701 gttttaaggt tctgatcacc gaccagtgat ggagaaacta tgagttcatc gtgtatagaa
     8761 gaagtcagtg taccggatga caactggtac cgtatcgcca acgaattact tagccgtgcc
     8821 ggtatagcca ttaacggttc tgccccggcg gatattcgtg tgaaaaaccc cgattttttt
     8881 aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa gttatatgga tggctggtgg
     8941 gaatgtgacc gactggatat gttttttagc aaagtcttac gcgcaggtct cgagaaccaa
     9001 ctcccccatc atttcaaaga cacgctgcgt attgccggcg ctcgtctctt caatctgcag
     9061 agtaaaaaac gtgcctggat agtcggcaag gagcattacg atttgggtaa tgacttgttc
     9121 agccgcatgc ttgatccctt catgcaatat tcctgcgctt actggaaaga tgccgataat
     9181 ctggaatctg cccagcaggc gaagctcaaa atgatttgtg aaaaattgca gttaaaacca
     9241 gggatgcgcg tactggatat tggctgcggc tggggcggac tggcgcacta catggcatct
     9301 aactatgacg taagcgtggt cggcgtcacc atttctgccg aacagcaaaa aatggctcag
     9361 gaacgctgta aaggcctgga tgtcaccatt ttgctgcaag attatcgtga cctgaacgac
     9421 cagtttgatc gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa aaattacgat
     9481 acctattttg cggtggtgga tcgtaatttg aaaccggaag gtatattcct gctccatact
     9541 atcggttcga aaaaaaccga tctgaatgtt gatccctgga ttaataaata tatttttccg
     9601 aacggttgcc tgccctctgt acgccagatt gctcaatcca gcgaacccca ctttgtgatg
     9661 gaagactggc ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga
     9721 ttcctcgccg catggccaga aattgcggat aactacagtg aacgctttaa acggatgttt
     9781 acctattatc tgaatgcctg cgcaggtgct ttccgcgccc gtgatattca gctctggcag
     9841 gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag gctattctat
     9901 cgccccctct tcgggggcga gttcagatca ggcttctgta cctggttgat tcatggcatt
     9961 ttctcgtgcc gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcgat
    10021 ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc
    10081 cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc
    10141 gtcgatgcca ataaatcctt tgtacaggat atatttcatc aactgactat cctggacttt
    10201 aaaccagatc tggcgattat tttccgaggt taatattttc gccacttcag cggtggtcat
    10261 aatatgacct gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac
    10321 gtttacccaa tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat
    10381 caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg
    10441 cgccacggaa gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg
    10501 cgtacgaaaa tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc
    10561 cgtaaacata cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg
    10621 tggaaaacgc ccttaccagt gaaggggtaa gaatggctat tttttcactg gagaattaat
    10681 aaatcctcgc tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc
    10741 ggctttttag tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag
    10801 tgaagcgcgt ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac
    10861 tgcgatgggt tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc
    10921 ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct
    10981 ggcattaacg ccggttatcg cgcaattaaa tggttccggt cgacgtgagc gcattgcgca
    11041 tcaggtgcga caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct
    11101 gtggaatgca ggttacatta tccgctccat ggaaaacatc gatccggctc tggcggacaa
    11161 agccgtgggt tatctgcgtg cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt
    11221 tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat
    11281 cggcctgctg gtgaacatcc cggtgaacta tatctttatt tatggtcatt tcggtatgcc
    11341 tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg tcatgttcct
    11401 tgccatggtt tcttacatta aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg
    11461 caccgctaag cccgatcctg cggttatgaa acgactgatt caactcggtt tgccgattgc
    11521 gctggcactg ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct
    11581 cggtattgtt gatgtcgcag gacaccagat tgccctgaac tttagttcac tgatgtttgt
    11641 gcttccaatg tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg
    11701 ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc
    11761 aaccctgacg gccattttca cggtttcact gcgggagcaa atcgccctgt tgtacaacga
    11821 caatcccgag gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat
    11881 ttctgactca atccaggtga ttggcagtgg gattttgcgt ggttataaag atacgcgttc
    11941 cattttctat atcaccttta cagcttactg ggtgctgggc ttgccaagcg gctatattct
    12001 ggcactgacc gatctggtcg ttgaacctat ggggccagca ggcttctgga taggctttat
    12061 tatcggcctg acatcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat
    12121 gccgtcagcc atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac
    12181 gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc
    12241 tgtaatttca gagcgttaca cagagtttgc cgactggcat ctgttatctc aacgcgtata
    12301 tccccgttaa cttccccttt cacattgatg aagtcaggtg aaatttcttt cgcactggtt
    12361 tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc
    12421 gcaccagtga tcagccacag ttttccgcca ttcagattga tgttaccaac aatgaaagtg
    12481 acgctgtcac cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta
    12541 atcgttgatt gttgcagctc tgcgccataa ccaataatca ttgaagcgcc gtccagtaat
    12601 gcaacactcc cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc
    12661 gcgctggtgg gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta
    12721 ccggcatgca cttcagcccg atcccccaca ttcaccgaca ggttttgcgt cttaccggaa
    12781 ctgtagagct gaaggccatc cgtccccaga cgataaatgg atccattctc aacaatagtg
    12841 tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct
    12901 ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc ttctccactt
    12961 ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc
    13021 aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc
    13081 aggttaccgc cattttccag caacaaaccg taggcataac cctgatcaac gctgaattca
    13141 ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg
    13201 gactgaacaa tatctgtggc aatagcaccg tcgctgaccg atagcgtgcc attgttaccg
    13261 tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc
    13321 acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc
    13381 gcgccaatga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc
    13441 gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt
    13501 gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt
    13561 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
    13621 cgcccttgca aatcaacaaa atacagccaa tttgcttaaa agctctgcaa acggtcgaaa
    13681 tagcgcagaa aattacgttt tgcctcttgc cacctttcca ctctgccgct aatattcgtc
    13741 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
    13801 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
    13861 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
    13921 attttcttaa tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta
    13981 aaagatttac actgtcttcg tctgcgttat cagaaggaga atcgctatgg caactttgtt
    14041 acaatttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc
    14101 acttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
    14161 aaagaaccac gaagccggtg ggatctacct tttcactgat gaaaaaagcg cccttgccta
    14221 tctggaaaaa catactgccc gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt
    14281 tttcgatgtc aatgagccac ttagtcaaat caatcaggca aaactcgcct gacagaattt
    14341 aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc
    14401 tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg
    14461 aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta
    14521 cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg
    14581 atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa
    14641 ataccgccga ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc
    14701 cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg
    14761 ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg acagcaaaaa
    14821 tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc
    14881 gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc
    14941 acgggtcacg tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg
    15001 tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg
    15061 agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat
    15121 aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg
    15181 tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattccctg cgagccctta
    15241 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg
    15301 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa
    15361 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
    15421 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
    15481 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
    15541 gaaattgttc ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg
    15601 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta
    15661 cgtgaagcgg ggatcattca tattctcgcc ctcggtgaag actacgaaat ggaaattaat
    15721 gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat
    15781 gcccgcggac agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa
    15841 ttacagaaaa caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc
    15901 gaagatattc gtggacgcgt agcgttcggc gcgttgccct ggctgatgca cgaccagcct
    15961 ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta
    16021 aagcctgcat cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac
    16081 tcaacaaact gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca
    16141 ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt
    16201 ccccctagcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca
    16261 ccgctggcaa tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag
    16321 ctggcgattt gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa
    16381 acgcgtactc gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc
    16441 gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta
    16501 aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca
    16561 gcggttgcga ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg
    16621 gcaaactgac tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa
    16681 acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga
    16741 ctctccgccc agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta
    16801 ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct
    16861 cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt
    16921 ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa
    16981 aagaggctta taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga
    17041 aacacatctc ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc
    17101 agcaatccat acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt
    17161 gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt
    17221 tgttttgccg ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc
    17281 ccaacggcat tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tcagcaaact
    17341 tcatgcaccg caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc
    17401 agcccac
//
LOCUS       DADMFL010000079        17389 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.080, whole genome shotgun sequence.
ACCESSION   DADMFL010000079 DADMFL010000000
VERSION     DADMFL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.080"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JNZ27_002730"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JNZ27_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY8664999.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3166)
                     /gene="hemX"
                     /locus_tag="JNZ27_002731"
     CDS             complement(1967..3166)
                     /gene="hemX"
                     /locus_tag="JNZ27_002731"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8665000.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(3188..3928)
                     /gene="hemD"
                     /locus_tag="JNZ27_002732"
     CDS             complement(3188..3928)
                     /gene="hemD"
                     /locus_tag="JNZ27_002732"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY8665001.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3925..4866)
                     /gene="hemC"
                     /locus_tag="JNZ27_002733"
     CDS             complement(3925..4866)
                     /gene="hemC"
                     /locus_tag="JNZ27_002733"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY8665002.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5253..7799
                     /gene="cyaA"
                     /locus_tag="JNZ27_002734"
     CDS             5253..7799
                     /gene="cyaA"
                     /locus_tag="JNZ27_002734"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY8665003.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7839..8159)
                     /gene="cyaY"
                     /locus_tag="JNZ27_002735"
     CDS             complement(7839..8159)
                     /gene="cyaY"
                     /locus_tag="JNZ27_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY8665004.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8207..9520)
                     /locus_tag="JNZ27_002736"
     CDS             complement(8207..9520)
                     /locus_tag="JNZ27_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY8665005.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9517..9822)
                     /locus_tag="JNZ27_002737"
     CDS             complement(9517..9822)
                     /locus_tag="JNZ27_002737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665006.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9844..10995)
                     /locus_tag="JNZ27_002738"
                     /pseudo
     CDS             complement(9844..10995)
                     /locus_tag="JNZ27_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11184..11387
                     /locus_tag="JNZ27_002739"
     CDS             11184..11387
                     /locus_tag="JNZ27_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8665007.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11424..12248
                     /gene="dapF"
                     /locus_tag="JNZ27_002740"
     CDS             11424..12248
                     /gene="dapF"
                     /locus_tag="JNZ27_002740"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY8665008.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12245..12952
                     /locus_tag="JNZ27_002741"
     CDS             12245..12952
                     /locus_tag="JNZ27_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY8665009.1"
                     /translation="MKQPGEELQETLTELDDRVVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12949..13845
                     /gene="xerC"
                     /locus_tag="JNZ27_002742"
     CDS             12949..13845
                     /gene="xerC"
                     /locus_tag="JNZ27_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY8665010.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13845..14561
                     /gene="yigB"
                     /locus_tag="JNZ27_002743"
     CDS             13845..14561
                     /gene="yigB"
                     /locus_tag="JNZ27_002743"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY8665011.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14645..16807
                     /gene="uvrD"
                     /locus_tag="JNZ27_002744"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14645..16807
                     /gene="uvrD"
                     /locus_tag="JNZ27_002744"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY8665012.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16954..17346)
                     /locus_tag="JNZ27_002745"
     CDS             complement(16954..17346)
                     /locus_tag="JNZ27_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665013.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4056 a         4480 c         4604 g         4249 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtt
     2041 tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcga gtctgcatca
     2101 gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag atattttgct
     2161 ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt
     2221 aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct tcctgatgac
     2281 gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc agatagatat
     2341 cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt
     2401 tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg atggaactgg
     2461 aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg gccagacgca
     2521 ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca taatccacct
     2581 gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg gtaatcagac
     2641 tcgggtcatt catatccgcc aggctggcgt ctgcactttt cagcaacgct gcagaggtcg
     2701 tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa tcggcctgag
     2761 ccagcagcca ggttttagca tcgctgccgg aaatggtggc gatcttttgt tggacttcat
     2821 ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgc gcagcttgtt
     2881 gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc aatgccgtca
     2941 gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt ttaccccagc
     3001 catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg agaatcaatg
     3061 cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg tccacggcct
     3121 ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg gcttcctgtt
     3181 atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa
     3241 tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg accaatagtc
     3301 gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag agttgctgca
     3361 acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag cgcatcgctt
     3421 cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa gtgacctcag
     3481 caccgcgcac cgtcagggta tccccaatta gctcacgacc gccattgcca cgtaatatca
     3541 gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact tcgctgattt
     3601 cccgatcctg tgggtagaga atcttctgtc cgcttacggt atgtagcgcc agtgcggtgg
     3661 tgcgtccaat ggtgaaataa gtaggtagtt ggggccattt acgatcttgc tgatgcagct
     3721 gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg ctctccccca
     3781 gcgctgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac tcaatcagcg
     3841 gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct tctccagcgg
     3901 gagacgggcg ggtgaccagg atactcatgc cggggcctct ccgttataga cttcagcgag
     3961 gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca tttgttcggc
     4021 atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac
     4081 cagcgcacat agccagattt cgccatcaat aagctcggcg tagctaccaa ttggcacctg
     4141 acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc
     4201 gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc ggcattcaat
     4261 accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat
     4321 gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg catcgtattc
     4381 gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc ggataatcag
     4441 atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc cgacgatact
     4501 gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat cgcgaggatc
     4561 ttcacgctcg caaatagtga ccagccccag accttgcggg aattcaaccg gcacatcttt
     4621 cattgaatgt acggcgatat cagcgcgatt ctccaggagc gcgacttcca gctcttttac
     4681 aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat cgccgcgcgt
     4741 caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact tgtctttgac
     4801 atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta aaacattgtc
     4861 taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt gccagagtga
     4921 tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg
     4981 gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata aacggtgcta
     5041 cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt
     5101 tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta
     5161 cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca
     5221 gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag actctgaaac
     5281 agagactgga tgccataaat caattgcgtg tggatcgcgc acttgctgct atggggcctg
     5341 cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat ccgctaatgc
     5401 cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct gatgaaactc
     5461 aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag gatccaccga
     5521 aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa
     5581 gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat agcgaagagc
     5641 gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg
     5701 aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc ggcagcctgg
     5761 ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt tatcgtactg
     5821 ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac gaagaagagc
     5881 attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca aatgaatggc
     5941 tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc agcctttggc
     6001 agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg ctgctggaag
     6061 cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc
     6121 acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg gagcgtgtta
     6181 ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt cgctgcttct
     6241 atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag
     6301 tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca atgctcgata
     6361 accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagctg ctcgacgcga
     6421 tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt agcgtctccg
     6481 ccagtccgca ggatatcggc gtactgacgc gtaaactgta tgccgcgttt gaagcattgc
     6541 caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa ccgaatctga
     6601 cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg tataaccgcg
     6661 cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt tacctgaata
     6721 aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt ttgtatatta
     6781 aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc
     6841 atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg tgtgagattc
     6901 gccatctggc gattatcgtt aacctggaat atgacccgac agctgcgttc cgcaatcagg
     6961 tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat caaaattgcc
     7021 tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact
     7081 tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa atgcatcagg
     7141 acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg cgcggcttaa
     7201 tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt tccagcaccc
     7261 gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct
     7321 tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt tatggcgcta
     7381 tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac gtcaaattac
     7441 cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttctttttc gaagaaacgc
     7501 aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt gaggtgtatc
     7561 accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc tactcgtcat
     7621 cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg cagttctatc
     7681 agattgtgaa ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa tcgattggca
     7741 acctgccgcc cgccaatcag gatcacgata cgccgctatt acagcagtat ttttcgtgat
     7801 gagcgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact gactgtttca
     7861 cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg
     7921 caaatccatt catcgccttt caggtcaaaa tggtagccgc cctgtttggt tgccagccac
     7981 acctggtgca gcggctcctg gcggttaata atgattttgc tgccgttttc aaaggtaatg
     8041 gtcagtacgc cgccgttgat ctcgcagtcg atatcgctgt cgccatccct gtcgtccaga
     8101 cgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact gtcgttcatt
     8161 gttgtaccct gtttttaagt gatggcggca gtatagcggc atggggtcag ggcttcaaag
     8221 tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata atgatgtctc
     8281 tgttttgaac ttcacgtacg ttctcctctc tgacacgagt tttcgccggg cagggggagg
     8341 gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca taagcgaagc
     8401 gatcgttttt agttagcgga ccgttaactg gaataatttg caagctcttt gaccgtaatt
     8461 ggcggataaa gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg accaagtctg
     8521 gcgtatccag cgggattatc tcaagctggt cttcaccaaa tgtcgataat tttgttacat
     8581 acagccgatc tttatattgc gcaatattgc cagtaatcag atggaattgt tcaggttcaa
     8641 tacctggtag gtctatccac ttgctccact gacggtcggg cgtgagctgt ttacgccagc
     8701 gaacgcgact ttcccctact tcaaaagaga cgtcatcatc tacgggtatc gctttgcctg
     8761 ataattgacc aaagggataa cgatgcagac ccttattgtc gcgaaaaacc agcagtgaat
     8821 gaggtatcca gcgaattatc tcaaatgagt ctgcattcgc cgtaaggtga acgccattta
     8881 aataaatatt ctgcccactg cggataagca gatcattgga atatgaattt ctgtttacac
     8941 tcataagacg tggattaata ccccaccatc ttttttctaa tacagtaaca tcactgctgc
     9001 tcccctgacg tcgaccggac aagtagagat ttttactatc caccagcgtg gtagagttac
     9061 cttcgatagc ttttagtgtc gccagatcaa cacgtgacgc gccgctatta ctttcggtgc
     9121 gctgtccgtc aaaatagagg ctatatttat cgaccgcgaa gcggcccata gcctgaaagc
     9181 tggcaatatc gacagtgggt gttcccggtg gattgctaac gattttgcca cgccagagaa
     9241 taacgcggcc gtctgtcacc cagtggtatt cctcattttc tatgcttatt tcatcggaag
     9301 gtatataacc cgttttccca gagatatttt tcacaaggcg caaaccggcc ctgctcatct
     9361 ccggaaactg ggcaatgatt ttcttatccc cccaataacc tttaccgtaa gacataacac
     9421 cgtgatcatt gtaatagtcg aaccctatgg cctgtacagc aggcgtcaga aatgtgtgtc
     9481 ccaacaggat gagtgcaact gtgattattt gacccttcat tttgtcacct cattgcgcgg
     9541 ggcaggaata aattcgccgt catcagtaat ttttccgtcc agatatcccg ttttgtaatt
     9601 tacgccatag acatatcccg aatgttgttc cttccagatt aagcgaactt gtttcggatc
     9661 agcggtgcgg tatcgccaga gctgcgcgcc atcaaagctg taaatgtagc ggttatcgcg
     9721 ggcataccat ctgcgcaaaa agggatggtc attaagatgg gcttcgcgtt catccggaga
     9781 acggttatcc agtaagatca gcggtccgtc agattcaaac acgtgtaaac cggaatggta
     9841 tggctaccct tcggcacgcg taagtaaata gccgtggcgt ttcccggcgt tgaaacgttt
     9901 gttgataata agattgggat catcaagttt cacgatctcc agcttacggt caccagactc
     9961 tgtttcgatg atggtataaa gcgagtcctg atactgggcg acagcatcac tgatgggttg
    10021 aaactgctcc ggatcgagtc cgggtaattc ttcctgttta caagcaggtc ctttacgcca
    10081 gaacactttc ttttccagca gagaaaaatc cagacagtgc tttgttaaat cttcatccca
    10141 cgccagattt tccttgtcga ggactttacg ctgcagcccg tttttatcac gccaggtcaa
    10201 gagtgagccg ggcatccagc gcacgacgga aaaggtgtcc gggtcggcat caagctgttc
    10261 gccgttgagc aggattttgg tccgtgtacg agccagggta tcccaggggc caaatggtac
    10321 ggcaatgcag gggttaaacg tggtggaaaa ctttcctcgc tgatcccagg acttctgcgc
    10381 cagcacacgg aaactctcgg ggtcagcgag gcgatgccca ttgatataca agaaattagc
    10441 gtcacgtaat atcagcccca gtaagtcagg atcccaggga ggccggaatt ccacctgatg
    10501 cagcgttttg atatccaagc tatttccacc accattgtca tcggtgcgct tgccttcaaa
    10561 atagagactg tgtttatctg cggcaaaatc accccatgcc ttgaacgagg cgacatcgac
    10621 gggaggtgtg ccgggagggt tttgcacaac ttcacccgcg tagaggatat tgcgaccatc
    10681 gctatgccat gaataatgag gatactaggc ctgtgcgccc tcatagaggt agtcggttat
    10741 caaatcagtc agaggcggtt ctttatccca ttcgtctagt gttgtcgggt ctattaataa
    10801 attgggtaac cgacgcaaac tcttgtaatt cacgtcaggt agagaaagat ttgtttcttt
    10861 ataagggcgc aggtacacca cgccttttcc ctgtttctgg taaatattat gttcaggtgc
    10921 ggaaagccga catccccatg ccgacgataa aaacaatgaa caagccaaca aacaaaccac
    10981 attgcgatag tgcataaagc catcctggcg cgaggtgttg atcacgaaac tgccagcaga
    11041 acataaatcc tcacgagtaa gcgttatact cgcagcattt cctcactttt cagacttcat
    11101 aaagagtcgc taaacgcttg cttttacgcc ttctcctgcg atgatagaaa gcagaaagcg
    11161 atgaacttta caggcaatcc ataatgaaaa acgtgtttaa ggcactcact gtattactta
    11221 ctctcttcag cctgacgggc tgcggtctga aaggtccgct ctatttcccg cctgcagata
    11281 aaaacgcacc gccgccgacc aaaccggtag agacgcaaac gcaatccacg gtgccggata
    11341 aaaacgatcg cgccactggc gatggtccat cccaggtgaa ttactaaaag tcagtttctg
    11401 tacccgcgtg attggagtaa atgatgcagt tctcgaaaat gcatggcctt ggcaacgatt
    11461 ttatggtcgt cgacgcggta acgcagaatg tctttttttc accggagctg attcgtcgcc
    11521 tggcggatcg gcacctgggg gtagggtttg accagctgct ggtggttgag ccgccgtatg
    11581 atcctgaact ggattttcac tatcgcattt tcaatgctga tggcagtgaa gtggcgcagt
    11641 gcggtaacgg tgcgcgctgc tttgcccgtt ttgtgcgtct gaaaggactg accaataagc
    11701 gtgatatccg cgtcagcacc gccaacgggc ggatggttct gaccgtcacc gatgatgatc
    11761 tggtccgcgt aaatatgggc gaacccaact tcgaaccttc cgccgtgccg tttcgcgcta
    11821 acaaagcgga aaagacctat attatgcgcg ccgccgagca gacaatctta tgcggcgtgg
    11881 tgtcgatggg aaatccgcat tgcgtgattc aggtcgatga tgtcgatacc gcggcggtag
    11941 aaacgcttgg tcctgttctg gaaagtcacg agcgttttcc ggagcgcgcc aatatcggtt
    12001 ttatgcaagt ggttaagcgc gagcatattc gtttacgcgt ttatgagcgt ggggcaggag
    12061 aaacccaggc ctgcggcagc ggcgcgtgtg cggcggtggc ggtagggatt cagcaaggtt
    12121 tgctggacga agaagtacgc gtggaactcc ccggcggtcg tcttgatatc gcctggaaag
    12181 gtccgggtca cccgttatat atgactggcc cggcggtaca tgtctacgac ggatttattc
    12241 atctatgaag caaccagggg aagaactgca ggaaacactc acggagcttg atgaccgggt
    12301 ggttgtcgat tatctgatta aaaatcctga gttttttatc cgcaatgcgc gcgcagtaga
    12361 agcgatacgt gtgccgcatc cggtacgcgg caccgtttcg ttggtcgagt ggcacatggc
    12421 ccgcgcacgt aatcacattc atgttctgga agagaacatg gcgttgttga tggagcaggc
    12481 tatcgccaac gaagggctgt tttatcgcct actctacctg cagcgcagtc tcacggccgc
    12541 cagcagtctc gacgatatgc tgatgcgctt tcaccgctgg gcgcgcgatc tcggcctggc
    12601 tggtgcgagt ctgcgcctgt ttccagatcg ctggcgctta ggtgcgccgt cgaaccacac
    12661 tcatctggca ttaagccgtc agtctttcga accgctgcgt attcagcgtt tggggcagga
    12721 acagcactat cttgggccgc ttaacggacc ggagctgctg gtggtgctac cggaagcgaa
    12781 agcggtggga tcggtggcga tgtcgatgct gggaagcgat gctgatttgg gtgttgtgct
    12841 gtttaccagt cgcgatgcca gtcactatca acaagggcag ggaacgcagt tacttcatga
    12901 aattgcgctg atgttgccgg agcttctgga gcgttggatt gaacgcgtat gaccgattta
    12961 cacaccgatg tagaacgcta cctacgttat ctgagcgtgg agcgtcagct tagcccgata
    13021 accctactta actaccagcg tcagcttgag gcgatcatca attttgccag cgaaaacggc
    13081 ctgcaaagct ggcaacaatg cgatgcagcg atggtacgca attttgctgt acgcagtcgc
    13141 cgtaaagggc tgggagcagc aagtctggcg ttacggcttt ctgcgctacg tagctttttt
    13201 gactggctgg tcagccagaa cgaactcaaa gctaacccgg cgaaaggagt ttcggcaccg
    13261 aaagcgccgc gtcatctgcc gaaaaatatc gacgtcgacg atatgaatcg gctgctggat
    13321 attgatatca acgatcccct cgctgtacgc gaccgtgcaa tgctggaagt gatgtacggc
    13381 gcgggtctgc gtctttccga gctggtgggg ctggatatca aacacctcga cctggagtcc
    13441 ggcgaagtgt gggtgatggg gaaaggcagc aaagagcgcc gcctgccgat tggtcgcaac
    13501 gctgtggcgt ggattgagca ctggcttgat ttgcgcgacc tgtttggtag cgaagacgac
    13561 gcgctttttc tgtcgaaact gggcaagcgt atctccgcgc gtaatgtgca gaaacgcttt
    13621 gccgaatggg gcataaaaca agggctgaat aatcacgttc acccacataa attacgtcac
    13681 tcgttcgcta cgcatatgct ggagtcgagc ggcgatcttc gtggtgtgca ggagctactg
    13741 ggtcatgcca acctctccac cacgcaaatc tatactcatc ttgattttca acaccttgcc
    13801 tcggtgtacg atgcggcgca tccacgcgcc aaacggggga aataatgcgt ttttaccggc
    13861 ctttggggcg catctcggcg ctcacctttg acctggatga taccctttac gataaccgtc
    13921 cggtgatttt gcgcaccgag cgagaggcgc ttacctttgt gcaaaattat catccggcgc
    13981 tgcgcagctt ccagaatgaa gatctgcaac gcctgcgcca ggcggtacgg gaagcggaac
    14041 ccgagattta tcacgacgtg acgcgttggc gttttcgttc gattgaacaa gcgatgctcg
    14101 acgccgggct gagtgccgaa gaagccagtg caggcgcaca cgcagcaatg attaactttg
    14161 ccaaatggcg cagccgaatc gacgtcccgc agcaaactca cgacacctta aaacagctgg
    14221 cgaagaaatg gccgctggtg gcgatcacca acggtaacgc ccagccggaa ctgtttggtt
    14281 tgggggatta ttttgagttt gtgctgcgcg ctggcccgca cgggcgctca aaaccgttca
    14341 gcgatatgta ctttttggct gcggaaaaac tcaacgtgcc gatcggcgag atcttacatg
    14401 ttggggacga tctcaccact gacgtgggtg gggcaattcg cagcggaatg caggcttgtt
    14461 ggatcagacc ggaaaatggc gatctgatgc aaacctggga cagccgttta ctgccacatc
    14521 tggaaatttc ccggttggca tctctgacct cgctgatata atcagcaaat ctgtatatat
    14581 acccagcttt ttggcggagg gcgttgcgct tctccgccca acctattttt acgcggcggt
    14641 gccaatggac gtttcttacc tgctcgacag ccttaatgac aaacaacgcg aagcggtggc
    14701 cgcgccacgc agcaaccttc tggtgctggc gggcgcgggc agtggtaaga cgcgcgtact
    14761 ggtgcatcgt atcgcctggc tgatgagcgt ggaaaactgc tcgccatact cgattatggc
    14821 tgtgacgttt accaacaaag cggcggcgga gatgcgtcat cgtatcggac aactgatggg
    14881 caccagccag ggcggcatgt gggtaggcac cttccacggg ctggcgcacc gcctgctgcg
    14941 tgcgcaccat atggacgcca atctgccgca ggatttccag atcctcgata gcgaagacca
    15001 gctacgcctg cttaagcgtc tgatcaaagc catgaacctc gacgagaagc agtggccgcc
    15061 gcgccaggca atgtggtaca tcaacagcca gaaagatgaa ggcctgcgtc cgcatcatat
    15121 tcaaagctac ggtaatccgg tggagcagac ctggcagaag gtgtatcagg cgtatcagga
    15181 agcgtgcgat cgcgcgggcc tggtggactt cgccgagctg ctgctgcgcg ctcacgaact
    15241 gtggcttaac aagccgcata tcctacaaca ctaccgcgaa cgttttacca atatcctggt
    15301 ggacgaattc caggatacca acaacattca gtacgcgtgg atccgcctgc tggcgggcga
    15361 caccggcaaa gtgatgatcg tcggtgatga cgaccagtca atctacggct ggcgcggggc
    15421 gcaggtggag aatattcagc gtttccttaa tgatttcccc ggtgccgaaa ttattcgtct
    15481 ggagcaaaac taccgctcta ccagcaatat tctgagcgcc gctaacgccc tgattgaaaa
    15541 caataacggg cgtctgggta aaaaactgtg gaccgatggc gcggacggtg agcctatttc
    15601 cctctattgc gcttttaacg aactcgatga agcgcgtttt gtggttaacc gcatcaaaac
    15661 ctggcaggac aacggcgggg cgcttgccga gtgcgccatt ctctaccgca gtaacgccca
    15721 gtcgcgtgtg ctggaagaag cactgttgca ggcgagtatg ccgtaccgta tttacggcgg
    15781 gatgcgcttc ttcgaacgcc aggaaatcaa agatgcgctc tcgtatctgc gcctgattgc
    15841 caaccgtaac gacgacgcgg cctttgagcg tgtggtgaat acgccaacgc ggggtattgg
    15901 tgaccggacg ctggacgtgg tacgtcagac atcgcgcgat cgccagttaa cactctggca
    15961 ggcatgtagt gaactgttgc aggaaaaagc cctcgccgga cgtgctgcca gcgccttaca
    16021 gcggtttatg gaactgatcg acgccttagc gcaggaaact gccgatatgc cgctgcatgt
    16081 acagactgac cgggtaatta aagactccgg cctgcgtacc atgtacgagc aggagaaggg
    16141 cgaaaaaggt cagacgcgta tcgaaaactt agaggaactg gtgacggcaa cgcgccagtt
    16201 cagctacaac gaagaagacg aagatttaat gccgctgcag gcgttcctat cccatgcggc
    16261 gctggaagca ggcgaagggc aggcggatac ctggcaggat gcggtacaac tgatgaccat
    16321 gcactcggcg aaaggtctgg agttcccgca ggtgtttatc gtcggtatgg aagagggcat
    16381 gttcccaagc cagatgtcgc tggatgaagg cgggcgtctg gaagaagaac gccgtctggc
    16441 ctacgttggc gtaacccgcg cgatgcagaa actgacgctg acctacgcgg aaactcgccg
    16501 tctgtatggc aaagaggttt accatcgccc gtcgcgcttt attggcgagt tgccggaaga
    16561 gtgtgtggaa gaggtgcgcc tgcgcgccac ggtaagccgc ccggtcagcc atcagcggat
    16621 gggtacgccg atggtcgaga acgacagcgg ctacaagctc ggccagcgtg tacgccacgc
    16681 taagtttggt gaaggcacca tcgtcaatat ggaaggcagc ggtgaacata gccgtttgca
    16741 ggtggcattc cagggccagg gaatcaaatg gctggtggca gcttacgccc ggctggagtc
    16801 ggtgtaacgt tgccggatgc ggtgctgcgc accttatttg gcctaaaaaa tcattcagat
    16861 tcaataaatt gcaacgtcat gtaggcagga taaggcgttt acgccgcatc cggcatcagc
    16921 agcatattta gtatctgaca caaagctatc attctaacct ttccgctcga cggaaatcat
    16981 ggtgacaaaa ggatagcgtt gccacgggat tgccccgcct ttcatataca tatgtgaaat
    17041 cgtgccacca agataaagca gctgctcaac gttcagtttc gctttggcat aacaggcaaa
    17101 atcataaaaa tttgtcgcct gctggctcaa caaaaacacg gcattcccat gtttattaat
    17161 cccaacaccg ttacgaattt tacgtgaggc gacgttggga tgaatacgcg gattaattac
    17221 accgttttcc atcaacattg gtcctgactg caccgcaaac tgaatatctt tactggcttt
    17281 aaaagcatcc agacgaacga tgccgacttt atctcccgcg acataaaaca cgccgccagg
    17341 acggataaag aaattccctt cacctgaagc gagatttaac gctaccttc
//
LOCUS       DADMFL010000080        17381 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.081, whole genome shotgun sequence.
ACCESSION   DADMFL010000080 DADMFL010000000
VERSION     DADMFL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.081"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(38..769)
                     /gene="rlmB"
                     /locus_tag="JNZ27_002746"
     CDS             complement(38..769)
                     /gene="rlmB"
                     /locus_tag="JNZ27_002746"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY8665014.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(949..3390)
                     /gene="rnr"
                     /locus_tag="JNZ27_002747"
     CDS             complement(949..3390)
                     /gene="rnr"
                     /locus_tag="JNZ27_002747"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY8665015.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEAALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3429..3854)
                     /gene="nsrR"
                     /locus_tag="JNZ27_002748"
     CDS             complement(3429..3854)
                     /gene="nsrR"
                     /locus_tag="JNZ27_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY8665016.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4059..5357)
                     /gene="purA"
                     /locus_tag="JNZ27_002749"
     CDS             complement(4059..5357)
                     /gene="purA"
                     /locus_tag="JNZ27_002749"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY8665017.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5461..5658)
                     /locus_tag="JNZ27_002750"
     CDS             complement(5461..5658)
                     /locus_tag="JNZ27_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY8665018.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5740..6744)
                     /gene="hflC"
                     /locus_tag="JNZ27_002751"
     CDS             complement(5740..6744)
                     /gene="hflC"
                     /locus_tag="JNZ27_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY8665019.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6747..8006)
                     /gene="hflK"
                     /locus_tag="JNZ27_002752"
     CDS             complement(6747..8006)
                     /gene="hflK"
                     /locus_tag="JNZ27_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY8665020.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8092..9372)
                     /gene="hflX"
                     /locus_tag="JNZ27_002753"
     CDS             complement(8092..9372)
                     /gene="hflX"
                     /locus_tag="JNZ27_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY8665021.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9448..9756)
                     /gene="hfq"
                     /locus_tag="JNZ27_002754"
     CDS             complement(9448..9756)
                     /gene="hfq"
                     /locus_tag="JNZ27_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY8665022.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9842..10792)
                     /gene="miaA"
                     /locus_tag="JNZ27_002755"
     CDS             complement(9842..10792)
                     /gene="miaA"
                     /locus_tag="JNZ27_002755"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY8665023.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10785..12632)
                     /gene="mutL"
                     /locus_tag="JNZ27_002756"
     CDS             complement(10785..12632)
                     /gene="mutL"
                     /locus_tag="JNZ27_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY8665024.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12642..13979)
                     /gene="amiB"
                     /locus_tag="JNZ27_002757"
     CDS             complement(12642..13979)
                     /gene="amiB"
                     /locus_tag="JNZ27_002757"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY8665025.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13998..14459)
                     /gene="tsaE"
                     /locus_tag="JNZ27_002758"
     CDS             complement(13998..14459)
                     /gene="tsaE"
                     /locus_tag="JNZ27_002758"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY8665026.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14431..15978)
                     /gene="nnr"
                     /locus_tag="JNZ27_002759"
     CDS             complement(14431..15978)
                     /gene="nnr"
                     /locus_tag="JNZ27_002759"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY8665027.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15977..17116
                     /gene="queG"
                     /locus_tag="JNZ27_002760"
     CDS             15977..17116
                     /gene="queG"
                     /locus_tag="JNZ27_002760"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY8665028.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17099..17152)
                     /locus_tag="JNZ27_002761"
     CDS             complement(17099..17152)
                     /locus_tag="JNZ27_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665029.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3937 a         4955 c         4369 g         4120 t
ORIGIN      
        1 catccaaatc tggatggctt ttcataattc agaggaacta gctgcgctga cgaaccgctt
       61 caaataagca aattccggtt gcaaccgaaa cgttcaggga agaaacactt cctgccattg
      121 ggatgctgat caactcatcg caatgttcac gggtcaggcg acgcatacct tcaccttccg
      181 cgcccatcac cagcgccagg cgtccggtca ttttgctctg atagagtgta tgatccgcct
      241 cgcctgccgt accgacgatc cagatattct cttcctgcaa catacgcatg gtgcgcgcaa
      301 ggttagtcac ccgaatcagc ggaacgcttt ctgccgcgcc gcaggctact tttttcgccg
      361 tagcgttgag ctgtgcggag cgatctttcg gcacaatcac cgcatgaacg cctgcggcgt
      421 ccgcactacg caggcacgcg ccgaggttgt gcggatcggt tacgccgtcg aggatcagca
      481 ggaacggttg atcgagcgaa gcgatcagat ccggcagatc gttttcctga tactgacgtc
      541 ctggcttcac gcgggcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat
      601 attggcggtt tgccaactgg ataaccacgc cctgagactc aagggcgtga atcagcggta
      661 acagacgttt atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc
      721 gctccagcag ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt aatgtactcg
      781 ttgtttacgt tttgcctgat gcactacgtt tatccggcct acatgatctc tgcaatatat
      841 tgaatttgcg tgattatgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa
      901 gcgcattttg tcagcaatct aaccctcttc ttttaaagag ggtattgatc actctgccac
      961 ttttttcttc gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga
     1021 tggctttttc gcttttctcg cgtctttctt cgccgctttc ggcttcgttt ttttctcacc
     1081 gcggaaggcg ctgtctggct caaagtttac ctttttaccg acctgacgac gcttgccgcc
     1141 ttttttgcct gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg
     1201 tgcgcgttca ctggagatca gactaaagtc gattttgcgc tcgtccatat taaccgcttc
     1261 gacgcgaact tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cccccatcag
     1321 gcgttgccct acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag
     1381 accatcaatg aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat
     1441 tacgccttta aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc
     1501 gacatcgcgc gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg
     1561 caacatctct tccatcgaat aatggtagcc gccggtttca gtggtgttgc cctgatgccc
     1621 ctgctctttc gccagcagat atttaatggc gcggtgcagc gtcaggtcag gataacgacg
     1681 aatcggcgaa gtaaagtgcg cataggactg caatgccaga ccgaagtgac cacggttttc
     1741 tggatcgtaa atcgcctgtt tcatcgagcg tagcagcatg gtttgcagca tttctgcatc
     1801 aggacggtcg gcaaccgact ccagcagttc cgcgtagtca cgcggttccg gcttgttacc
     1861 acccggcagc tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt
     1921 gctcggcttg tcgtgaatac ggaacagtgc cgcttctttc gctttctcaa cgaaacgcgc
     1981 cgccgagata ttcgccagaa tcatgcactc ttcaattaat ttgtgcgcgt cgttacgctg
     2041 ggtctgttcg atacgttcaa tacggcgttc agcgttgaaa atgaacttcg cttcttcgct
     2101 ctcaaatgag atcccaccgc gttcttcacg ggctttatcc agcactttat agaggttatg
     2161 caactcttcg agatgcttaa ccagcggggc gtactgctcg cgcagatcct gatcgccctg
     2221 cagaatatgc cagactttgg tgtaggtcag acgcgcgtga gagctcatca ccgcttcgta
     2281 gaatttgtag cccgtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag
     2341 gcggtctacc tgcgggttga gcgaacacag gccgttagag agcacttccg gcagcatcgg
     2401 gataacctgc gaagggaagt acaccgacgt gccacggtta cgcgcttctc tgtccagcgg
     2461 cgttggcgga cgcacatagt agctgacgtc ggcaatcgcg acccataaac gccagccgcc
     2521 gccgcgtttt ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat
     2581 ggtgaccagc ggtaaatcgc gcaaatcgac acggcccgct tttgcttctt ccggcacttc
     2641 ttctttcagc cccgcaacct gttgctcaac agcctgcggc cagatatacg gaatttcatg
     2701 ggtacgcaga gcgatatcaa ccgccatgcc ggtgcccata ttgtcgccca gcacttcgac
     2761 gattttaccc accgctttgg tgcggcgagt cggacgctga gtcagttcga ctacgaccac
     2821 aaagcccatc cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg
     2881 gctatcgtca ggaaccacaa agccgacgcc cgcttcggta aagtagcgac caacaatctg
     2941 gctggttttt ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc
     3001 acccagcggc tgcgccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga
     3061 gagatacaaa tcatctttac gcccttcaac ccgcagaaag ccgtagccat cacggtggcc
     3121 aataacggta cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacgacgagt
     3181 gaagaccagt tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg
     3241 ctcttcgcct tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg
     3301 tttggttaaa tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc
     3361 ttcgcgttcc tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa
     3421 gattttcgtc actccaacag caataattta taaagcggtt gattctcttc aaccaaatcg
     3481 gcaagcgtgt agttatccag ttccgtaaga aaactttgca cggccttaga aagcgcctgt
     3541 ttcaaccgac aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag
     3601 ggctccagct cgcgcaccac atcaccaata cgtatcgcac tcgccggttt acccaggcga
     3661 atgccgccat tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt
     3721 attttgacca tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc
     3781 atccgccctt ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa
     3841 ctcgttaact gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt tatattgatg
     3901 ataaaccagc cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag
     3961 gatttaacac tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt
     4021 tagcccggca ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cgggtcgcgc
     4081 agaatcatgg tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccggtc
     4141 agctcttcaa tacgcttgat gtagttcagc gccgcctgcg gcaggccgct acgatctttc
     4201 acgccgaaag tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc
     4261 cagtcgtcag ctgccagcgg agtggtagtc acttcgcgac catccggcat acggtaagcc
     4321 acgcagagtt taacctcttt caggccatcc agaacgtcca gtttagtcag gcagaagcca
     4381 gacagggagt tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga
     4441 cgacgacgac ccgtagttgc accgaattcg ttaccctgct tgcagaggaa ctcgccagtt
     4501 tcatcaaaca gttcggccgg gaaaggacct gcacctacac gagttgagta agctttgagg
     4561 atacccagaa cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca
     4621 gcagtggtgt tggaagaagt tacgtacgga taagtaccgt ggtcgatatc cagcagcgta
     4681 ccctgcgcac cttcgaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca
     4741 gaaacgtcaa ccaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact
     4801 ttctggtaat caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc
     4861 atcacttctt tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga
     4921 ccgcgacgtg ccactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc
     4981 gctttcgcgc cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc
     5041 agcggacatg cttcagacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt
     5101 tctttcatct ctttcatcag cgcggccgga gacagcacaa caccgttacc gatgatgctg
     5161 gttacattct cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg
     5221 attacgagag tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt
     5281 tcagtcagaa gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg
     5341 acgacgttgt tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc
     5401 tttttcagag ttacagcact ttttgcactc aaaaatgacc tcgaacagtc tgttttttgt
     5461 tcagccaatc gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc
     5521 gccaaaacga cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca
     5581 tgccttcggg taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag
     5641 ccagattgtc gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag
     5701 gatgcggtgg ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg
     5761 cgtcttcatg tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc
     5821 agagaagctg ttctcataag cacgcaggct acggatgaat gcgtagaagt ccggatcttt
     5881 actgaatgca tcagcaaaca gtttggctgc ttcggcatcg ccttcaccac gcataatgcg
     5941 gccctgacgc tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag
     6001 tttttccgct tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc
     6061 gcgcatacgg ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttgatacg
     6121 cacatcgaca acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt
     6181 gcccttcgtc tcagccgtta cgcgctctgc cgcttcggca atggcgttat ctgctgccgg
     6241 ggtagtaact tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag
     6301 ggtcagacga ccacgggaat cggtgacgat atctttcacg tccaggcgac caatttcaga
     6361 acgcagacgg tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc
     6421 acccgttgcc aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagagtcgac
     6481 gatcaggtct ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat
     6541 acgtgcgtcg agcattttca ccgtttcaat gaacggtatc ttgaaatgca gaccaggctc
     6601 ataaaccaaa ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc
     6661 gcgctcacct tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat
     6721 aatcgcgata actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc
     6781 gcgttggcgc ggcgttggtc cataatatcg ccctgactgg tggacgacgt gttgcttgct
     6841 ccactggttg tggaggaaga ggctggcggc agacgcagca gattgctggc accgttatcg
     6901 ctcttcgccg caggggcgtt accacctttc agcatctggt ctaacggcag aaccatcagg
     6961 ttgccacctt tatcgttaac cagcactttg cgggtgttac ccaacacttt ttccatcgtc
     7021 tcgatataca gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta
     7081 gcaaagcgcg ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc
     7141 tcttcgagga tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct
     7201 tctgcttcac gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc
     7261 gcttttactt cttccggcgg acgagcagcc tggaagttga cgtccagcag cgtgataccc
     7321 atgtcatacg gacgaatcgt ctcttccagt tcgcgctgag tatcgctacg aatcacggta
     7381 cgaccttccg tcagaatgcg gtccatggtg tatttaccga taactccacg cagggcgctg
     7441 tcggtagcct gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga
     7501 ttggtgacgc ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc
     7561 atcacaccag aagcggccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtcg
     7621 ataaacgtcg gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt
     7681 gttaccacgc cgcgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg
     7741 acaatcgctg ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc
     7801 gatgaactgc caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg
     7861 ctcagtttgc ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt
     7921 ccatttccct cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg
     7981 ttattaccgg gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct
     8041 gttgtctttc gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag
     8101 gtaatcgatc aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg
     8161 aacttgcaga cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg
     8221 ataaaaacga ctcctcagac gcccttcctg cggtggcaga cgcaatgtat gctgcgccac
     8281 ctcgccggaa agccgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg
     8341 tgcggaaagc cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa
     8401 atcttccagc atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat
     8461 ctcttcaaga accgtattca ccgcttcgat gttttcttgt acacgcacat ccgccgcatc
     8521 aatgacgtgc agcagtaatg tggcttgccg cgtctcttgt aacgtggctt taaatgctgc
     8581 caccagatcg tgcggcaggt ggcgaataaa ccctacggta tctgcaagta cggtttcacc
     8641 gacatctgca acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc
     8701 cgcgtagacc cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata
     8761 tcccaccagc gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg
     8821 ctgcttttca actctttcca ggcgcgactg tatctgcacg atgcgattac gcaacaaacg
     8881 acggtcggtt tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct
     8941 ttcaaggtgg gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgccagctc
     9001 aacctgcaac ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc
     9061 ggtgcggtcg ataacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct
     9121 cagggcatgg tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt ccgcaatttc
     9181 aactgcttta ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat
     9241 cacctgcaat gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc
     9301 catatctttg tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata
     9361 acggtcaaac aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct
     9421 ccccgtgtaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
     9481 agtattctgt gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt
     9541 actgtgatga gaaaccgggc gagatgggac aacagtagaa atcgcgtgct tgtaaaccat
     9601 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
     9661 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
     9721 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
     9781 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
     9841 ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
     9901 cactgtcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
     9961 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa
    10021 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
    10081 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
    10141 aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
    10201 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
    10261 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
    10321 ctgccgcaac cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
    10381 ccgcctgttg ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc
    10441 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
    10501 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
    10561 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
    10621 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
    10681 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
    10741 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
    10801 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
    10861 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    10921 tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    10981 acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    11041 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    11101 cccaattccg ctagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact
    11161 tttagccgca acggaatcag cagcggctgg gcgcaaacgg gcgcttcacc cggcgtcaat
    11221 tgtgcctgac gcagccaacg ttctgccact ggcaaggata aaagtgaaat gttgccatcg
    11281 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    11341 ttcgccgcaa gtgcaggttc ctgcggttcc ggcgcttttg gtttttgcat cggcgcgggc
    11401 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc
    11461 ggccaggggg cagccggacg actgcctgag gcgggcgcag gagagtagcg cggagctacc
    11521 ggctcacgag ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccggg
    11581 atggcacgcg gtgcgggttg tggttcatcg tccagcggta gcggcgtttc cagttgctgt
    11641 tgtagcacgc tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa
    11701 cgcacttcgt gtttggcggg gtgtacgttg acgtccacct gatgcgggtc gatctccaga
    11761 tacaacacaa atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc
    11821 gcatgattga tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc
    11881 gccagtgcgg gcgtagtgtg atttggatcg gccacccagc cgcgcagggt gagatcgccg
    11941 tgttgccatt caatcgccag cgcttgttcg agaaaagcgg tgccgcaaat cgcgcctaag
    12001 cgccgttctt tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta
    12061 tgcgacaggt tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatttcatca
    12121 atatggctaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    12181 aacagatcca gcacctccag cgtcgttccc accggatgcg ccgccggttt aaccgtcacg
    12241 tccatatcgc gtccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    12301 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    12361 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgagccagc
    12421 gccagcgcca gctcatcttt tttgataccg cagccgttat cacgaatgcg gataagtttc
    12481 gccccaccgc gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc
    12541 actagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    12601 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga
    12661 tctggcgtcg tcaccgtact ggcagtttgt gccgttgcac cctgcggcgc agattgcatc
    12721 ggatgcgcaa ggaagtaatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg
    12781 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    12841 agtactgatg ggatatccgg cgagcgcaga acgccaaggc tggcgtgttc tggtcgacgt
    12901 ttatgtattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    12961 acccgctggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac
    13021 tgactgttcg ccagcacatc acccgcccca cccagcagct ccgactgttt ctcgtgctgc
    13081 tccagccagc tggccatttc actgttggcg cgacggttag agagcaccaa tacggaagcg
    13141 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt
    13201 tgcttatgtg ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt
    13261 aaaacgcctt taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    13321 atggtgacat ttttctcccg cgtaccaccg ggaccgatag cgccagggtc ctgaccgccg
    13381 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
    13441 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    13501 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc
    13561 gcaaccacgg gcggcggagg aggcggtggc ggcacatcgg cgttaatcgt aaagacgaca
    13621 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg
    13681 accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga
    13741 ttattgccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    13801 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    13861 gttatccgtg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
    13921 accggcgtgc acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc
    13981 ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa
    14041 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    14101 gggtcaggaa gaacacctgt accttgttgt ggccactcca caaggcagat ggcatcgttg
    14161 gcaaaataat cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    14221 tcaaagtgat agaccattaa gttgtcgagc gtatagggtt cgaccagcgt ataagtgggg
    14281 cttttgacat taccctgatg acccagagcc tgtaaaaagc cccggctaaa ggtggtttta
    14341 cctgcgccta aatcgccata cagatagatt acggttgctc catcgcaggc tttcgctacc
    14401 cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg
    14461 gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    14521 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac
    14581 acagcctgca caggctgcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    14641 accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat
    14701 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    14761 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    14821 ggaacagcct aacaaccgtg cggcctcgct aggatgcggc gtgatcacgc gattgtgacg
    14881 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    14941 aaaattctca actttttgca gggctttttt cccccactct tgctggccca gaccgggacc
    15001 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatag tcagttcatg
    15061 caccatcaat tctggtcgtg cagtcagcag cggcgcaatg ttctcactgc gggtcagtac
    15121 tcggactaaa ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc
    15181 cgtgccgtga tcgccaccga taatcaccag ccgcccgtga tcgcctttat gcgaagtcgg
    15241 gcgacgcggt ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc
    15301 ctgacctgcc agccagctat ccagccccag tgagtcaaaa tgcagttgtc ctgtaacatc
    15361 ccgcgctttt ccagtgagca agcctagttt cagcgcaata aaagtgatgg tgtgatcggc
    15421 gttgatcact gcgcctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac
    15481 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    15541 gggcgcttgc tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
    15601 ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag
    15661 cgcggcctct tccggcaacg gtttgtcgct ctcctgggcc aacaacgtga cctcaatgcc
    15721 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
    15781 cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
    15841 gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc
    15901 tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt
    15961 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    16021 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    16081 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    16141 ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttacccggta cgctgcgtgt
    16201 aatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    16261 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    16321 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    16381 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    16441 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg
    16501 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa
    16561 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    16621 tgctcgccgc tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt
    16681 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
    16741 gaatcgctat tcgcaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc
    16801 accggaactc attgagttat ttgcctggag cgaagagaag tttttaaaag tcacggaagg
    16861 atcggcgatt cgccgtatag gtcacctgcg ctggctgcgt aatatcgccg tggcattagg
    16921 caatgcccct tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc
    16981 acttctcgat gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg
    17041 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt
    17101 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    17161 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttgat ttttaactta
    17221 tttatcaata ggttaaaaaa taccattcac cgtgcgaagt ttctttgaaa ttaagattac
    17281 agcctgaggc tgtggataac tctgtttaca agagtttgtc agagacaaca aaaaccagca
    17341 ataacgcatc gcgtcgctgt ggatatttta ttgagagaag a
//
LOCUS       DADMFL010000081        17342 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.082, whole genome shotgun sequence.
ACCESSION   DADMFL010000081 DADMFL010000000
VERSION     DADMFL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.082"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNZ27_002762"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNZ27_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8665030.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="JNZ27_002763"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="JNZ27_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8665031.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="JNZ27_002764"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="JNZ27_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8665032.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="JNZ27_002765"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="JNZ27_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8665033.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="JNZ27_002766"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="JNZ27_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /locus_tag="JNZ27_002767"
     CDS             complement(2739..3272)
                     /locus_tag="JNZ27_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8665034.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVMNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4495)
                     /gene="blaEC"
                     /locus_tag="JNZ27_002768"
                     /pseudo
     CDS             complement(3361..4495)
                     /gene="blaEC"
                     /locus_tag="JNZ27_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            complement(4558..4917)
                     /gene="frdD"
                     /locus_tag="JNZ27_002769"
     CDS             complement(4558..4917)
                     /gene="frdD"
                     /locus_tag="JNZ27_002769"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8665035.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4928..5323)
                     /gene="frdC"
                     /locus_tag="JNZ27_002770"
     CDS             complement(4928..5323)
                     /gene="frdC"
                     /locus_tag="JNZ27_002770"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8665036.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5334..6068)
                     /locus_tag="JNZ27_002771"
     CDS             complement(5334..6068)
                     /locus_tag="JNZ27_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8665037.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6061..7869)
                     /gene="frdA"
                     /locus_tag="JNZ27_002772"
     CDS             complement(6061..7869)
                     /gene="frdA"
                     /locus_tag="JNZ27_002772"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8665038.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8194..9171
                     /gene="epmA"
                     /locus_tag="JNZ27_002773"
     CDS             8194..9171
                     /gene="epmA"
                     /locus_tag="JNZ27_002773"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8665039.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9390..10892
                     /gene="yjeM"
                     /locus_tag="JNZ27_002774"
     CDS             9390..10892
                     /gene="yjeM"
                     /locus_tag="JNZ27_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8665040.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMMRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11043..14354)
                     /gene="mscM"
                     /locus_tag="JNZ27_002775"
     CDS             complement(11043..14354)
                     /gene="mscM"
                     /locus_tag="JNZ27_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8665041.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RTRGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIHIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14376..15344)
                     /gene="psd"
                     /locus_tag="JNZ27_002776"
     CDS             complement(14376..15344)
                     /gene="psd"
                     /locus_tag="JNZ27_002776"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8665042.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15441..16493)
                     /gene="rsgA"
                     /locus_tag="JNZ27_002777"
     CDS             complement(15441..16493)
                     /gene="rsgA"
                     /locus_tag="JNZ27_002777"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8665043.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16588..17133
                     /gene="orn"
                     /locus_tag="JNZ27_002778"
     CDS             16588..17133
                     /gene="orn"
                     /locus_tag="JNZ27_002778"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8665044.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4002 a         4466 c         4547 g         4327 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgctgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 attacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg gggtaaattg ctttgcaccc agcgggccat atcttcaatg gtcgacttca
     3781 caccataagc ttcagcatct aacgcccctg gcgaaacatg cactgcctta ccttcgcgat
     3841 atccccaggc gtaattcttt tcttctgggg gaggtacatt aatccacgta tggttgagtt
     3901 tgagtggctg gaagacacga gtttgcatcg cctgctcaaa actcaaacca gacggcttca
     3961 cagccagtgc gccgaacaaa ccgatactgg agttggcata cagacgttgt gttcctggag
     4021 cccatgcagg ctgccagttt tgatagaagc gcagcaagtc gcttgaggat ttcacttcat
     4081 ccggcacctg caatggcagg ccgccagcgg tgtaggtcgc aagatgtaat agtgtgatcc
     4141 cattccactg tttagcggta agttcaggcc agtattttgt tgcgggatcg cttaacttga
     4201 tttcccctcg agcaatagcg tcgccaccaa gcacgcccgt aaatgttttg ctgaccgaac
     4261 ctaactcaaa caacgtttgc tgtgtgacgg gctgcttttt ggcgatgtcc gcatagcccc
     4321 aggtaaagta ataaggttta ccctgataaa ttaccgccac cgccataccg gggatctttt
     4381 gttgctctat aagcggggta attgtgcgat gcacaatatc gttgatttgt tgaggggcag
     4441 caaatgtgga gcaagaggcg gtaattaata aggcgcagag cgtcgttttg aacataaggt
     4501 ctggtttcca tacaaaacgg cccgccatag gcgggccgga tttacattgg cgatgcgtta
     4561 gattgtaacg acaccaatca gcgtgacaac tgtcaggata gcagccagac cgtagaaaac
     4621 ccatttgccc gcaggcacgt ggattttcag atcgtgcatc gcgtggtgca tacggtgtaa
     4681 accacaccac agcggcagaa cgatcatcag gaacaggaat acgcgaccaa tgaagctctg
     4741 cgcgaacgcc agaacgcgct cgtagctcag cgcatcaccc ggaaacagcc ccagtggcag
     4801 cagaataccc accagcagga tcatcaccgg cgcaataatt gcgctccaca taccaccggc
     4861 cccgaagagg ccccagaata ccggttcgtc agaacgcttt ggatttggat taatcatctc
     4921 aggctcctta ccagtacagg gcaacaaaca ggattacgat ggtggcaacc acagttaccg
     4981 cccagagact tttgataatt ggctctggtc ccattttttc gtcttttaca atgatattgg
     5041 ccgctttcgg tgccagttca aaccaggttt tggtgtgcag cagagctgcc gtcagagtga
     5101 tcaggttaat gatcacgata accgggtttt gtaaaaagtc gacgaatccc gcccaggctt
     5161 ccgggccatt tttcagggca aacagcccga aaatcagttc aatgctgaac cacacagccg
     5221 gaaccgccgt gccttcgcgc agcatgtaaa agcgataaaa cggcaatttt ttccaccagg
     5281 tggacgtcat tggccgtaca tacggtttac gtttagtcgt catgttgcac tccttagcgt
     5341 ggtttcaggg tcgcgataag aaagtctttc gaactttcta ctttgccctg ctgaatggcc
     5401 gcagccggat cgacgtgttt cgggcagact tcggagcaat agcccacgaa agtacagctc
     5461 catacaccgt tctggctgtt caactgcgcc atacgctcct tcttaccgtg gtcgcggcta
     5521 tcttcgttat aacgatgcgc cagcgtaatg gcagccggac cgatgaactc tgggttcagg
     5581 ccaaactgcg ggcacgcggc gtagcacaga ccacagttga tgcaaccgga gaactggtga
     5641 tactttgcca tctgcgccgg ggtctggatg ttagtaccct gatccgcggt gcgggagttg
     5701 ccgatgatgt acggtttgat cgcttccaga ctttcgatga agtgggtcat atcgaccacc
     5761 agatcgcgtt caatcgggaa gttagctaac gcttcaacct tcataccgtc ggtgtaatca
     5821 cgcaggaagg ttttacatgc cagttttggc acgttgttaa ccatcatgcc gcaggaacca
     5881 caaatcgcca tacggcagga ccagcggtaa ctcaggtccg gtgccaggtt gtctttgatg
     5941 tagcccagcg catccagtaa tgaggtagtt gcgtcataag gcacttcata gaatgcgcta
     6001 tgcggtgcgg tatcgacttc cgggttatag cgcaccacct caattttcag gtttttcatc
     6061 tcagccattc gccttctcct tattattggc tgcttccgcc ttatcggctg catccgcttc
     6121 gccaccgtaa acgcgtttag ctggcggcag cgtagtaatc ttcacgtcgc tgtactccag
     6181 gcgagtcgtg ccatcagcat cgcggaaggc gagggtgtgt ttgagaaagt tgacgtcgtc
     6241 acgctcggtg caaccttcgt ccagacgctg gtgtgcgccg cgggactctt tacgtgccat
     6301 tgcggagtgc gccatacatt cagcaacgtt cagaccgtgg ctcagttcaa tggtgtagag
     6361 caggtcggtg ttgaacacgc tggaggtgtc ggtgatgcgc acgcgcttga agcgttcctg
     6421 cagctctgcc agcttgtcga tggttttctg catcagttcc ggcgtacggt agataccgca
     6481 accttcttcc attgccaggc ccatttcgtc gcggatcttc gcccagtttt cgccgccatc
     6541 ctggttaacc agatctttca gacgttgttc aacgccagct gcctgcgctt caattgccgc
     6601 ttcgttgcca ttaccggcag ttgctgcacg ctctgtcgct tgttcaccgg ccagacggcc
     6661 gaagaccacc agttccgcca gggagttgga gcccagacgg tttgcaccgt gcagaccaac
     6721 agaggaacat tcacccacgg cgaacagacc tttaatgcgg gtttcacagt tctgatcggt
     6781 ttcgataccg cccatggtgt agtgtgcagt cggacgtacc ggaatcggtt ctttaaccgg
     6841 atcgacgcca acgtacgctt tcgccagttc gcagatgaac ggcagacgtt catgcagttt
     6901 tttctcgccg aggtgacgca ggtcgagata aaccacatca ccacgcggcg tggagatggt
     6961 gttgccttta cgccattcgt gccagaaggc ctgagaaact ttgtcgcgtg gacccagttc
     7021 catatatttg tttttcggct cgcccagcgg agtttccggg cccatgccgt aatcttgcag
     7081 ataacggtag ccatttttgt tgaccagaat accgccttca ccacggcagc cttcggtcat
     7141 caggataccg gaacctggca gaccggttgg gtgatactga acgaattcca tgtcacgcag
     7201 cggaacgccg tggcttagcg ccatacccat accgtcaccg gtaacgatgc cgccgttggt
     7261 gttgtaacga taaacgcgac ccgcaccgcc ggtagccata acgaccgcgt tagcacggat
     7321 ctgcaccaac gtgccttcca tcatgttcat tgctaccagg ccgcgaacat gaccatcatc
     7381 aaccagaata tccagcacga aatgttcgtc aaaacgctgg atctgcggga attgcagaga
     7441 ggtctggaac agcgtgtgca gcatatggaa gccggtctta tcggcggcga accaggtgcg
     7501 ctcgattttc atgccgccga agcgacgtac gttgacgcta ccatccgggc gacggctcca
     7561 tgggcatccc cacagttcca gttgggtcat ttcggttggg cagtggtgga cgaaataatc
     7621 cacgacatcc tgctcacaca accagtcgcc acccgctact gtatcgtgaa agtgatattc
     7681 gaagctgtca tgatcctgcg cgacagcggc ggagccccct tctgcagcaa cggtatggct
     7741 acgcatcggg tatacttttg agattagtgc gatttttgca tttggatttg cctgcgcggc
     7801 agcaattgca gcacgtaatc ccgcgccacc ggcgcctaca atggcaagat cggcttgaaa
     7861 ggtttgcacg acattcctcc agattgtttt tatcccacag ccccgtactt cagggtaagt
     7921 gcctgaaagt tacggtctgc gaacgctatt ccactgctcc tttataggta caacagtata
     7981 gtctgatgga taagtctgaa atttgacgag atcgattttt ttagtgcgca agagggctaa
     8041 attatcactg aagatgatta atttaattac taaaccatca gatcgtgctt tttttagtca
     8101 ctgcccaccg ctgtttgatt cctgcgtaaa atttgtctcg ccgtcgcgtt gcgagtagac
     8161 ttcgtgccct tgtcaaaaac tggagattta actatgagcg aaacggcatc ctggcagccg
     8221 agcgcatcca ttcctaactt attaaaacgc gcggcgatta tggcggagat ccgtcgtttc
     8281 tttgccgatc gtggagtgct ggaggtggag acgccttgta tgagccaggc gacggtaacc
     8341 gatattcatt tggtcccgtt tgagacacgt ttcgttggcc ccgggcattc gcaggggatg
     8401 aatctctggt taatgaccag cccggaatac catatgaaac gcctgctggt tgctggttgt
     8461 gggccggtat tccagctgtg ccgcagcttc cgtaatgaag agatggggcg ttatcacaac
     8521 cctgagttca ctatgctgga gtggtatcga ccgcactatg atatgtaccg gttgatgaac
     8581 gaggtggacg atctcttaca acaggtgctg gactgcccgg cagcagaaag cctttcttat
     8641 caacaagctt tcttgcgtta tctggaaatt gacccgctct ctgccgacaa aacgcaactg
     8701 cgggaagtcg cagcgaaact ggatttgagc aatgttgctg ataccgaaga agaccgcgac
     8761 acgttgctac aattgctgtt tacctttggc gtagagccaa atattggtaa agaaaaaccg
     8821 acctttgtgt accactttcc agccagccag gcatcactgg cgcaaatcag taccgaagat
     8881 catcgggtcg ctgaacgctt tgaggtttat tataaaggta ttgagctggc gaatggtttc
     8941 catgaattaa cggatgcccg tgagcagcaa caacgctttg aacaagataa ccgtaagcgc
     9001 gcggcgcgcg gtttgccgca gcaccccatt gaccagaatc tgattgaagc cttgaaagtc
     9061 ggtatgcctg actgctccgg cgtggcatta ggtgttgatc gtctggtgat gttggcgctg
     9121 ggcgcggaga cactggctga agtcatcgcc tttagcgttg accgggcata attctgaaaa
     9181 attactgaat taacagcgaa gaatggcgtg ataagcgcca ttctgtagca tatgttttca
     9241 tttttccgtt aaaggccaca atttaagact ggatatttga tatcatcaag gtatcaattc
     9301 tgtattggtt tttacgctgc ctgctcaatg ttgcgcagtg cagttcagtg ggcacgtgtt
     9361 atacacgcgc tgaaatgaag gatggtttca tgcctcacac gataaaaaag atgagtctga
     9421 taggactcat attgatgatc tttacttccg tatttggatt tgccaatagc ccatcggctt
     9481 attacttaat gggttatagt gcgattccct tttatatatt ttctgcattg ttattcttta
     9541 ttccattcgc cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta
     9601 tctattcctg gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt
     9661 ggttttcctc ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct
     9721 caacattcct ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta
     9781 cgcaggtggt tggtctgctg gcagtagcat ggatgattct ggtcaccgtc gttgcttcaa
     9841 aggggattaa taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga
     9901 atttagtatt gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc
     9961 aggatattaa tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat
    10021 cgtttgtggt atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg
    10081 ataaaacgga aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta
    10141 tttcaatcgg ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg
    10201 tattaagtaa tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg
    10261 gggtgacgct gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat
    10321 ggtttgcgcg tattaccgga ctttcgatgt tcctcgctta taccggtgcg ttctttacgc
    10381 tttgctattc accgctgaaa gccatcatcc aggggacgcc gaaagcgttg tggccggaac
    10441 cgatgatgcg cctgaatgcg atggggatgc cgtctatcgc catgtggatg cagtgcgggt
    10501 tggttactgt cttcattctg ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta
    10561 acaagctgac gctgatggcg aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg
    10621 ctttcccgtt ttttaaagca cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc
    10681 gtatgtcggc aatgattgcg acggtggttg tcgttctggt ggtgacattt gcgaacgtct
    10741 tcaccattat tcagcctgtg gttgaagccg gagactggga cagcacattg tggatgattg
    10801 gcggccctgt cttcttctcg ctgttagcga tggcgattta ccagaactat tgcagtcgca
    10861 tggcgaataa acctgagtta gctctcgact gatcatatga aatcctgcaa ctcatcgtct
    10921 ctccggcgat gagttgtcag gaagtaatgc gtatggatga tactctccag ggccggtgat
    10981 aactgaagat gatgttaagc tgctaaatga gccacgttga catcatttgg cagcttcatg
    11041 cattacaaac ttcccgcctg acgacctttg cccgcagacg tcagcgttct ccccgtttgt
    11101 ttaccgttga ggctttccag acgcatctgg aagggcggga atggcatatc gataccgtgg
    11161 gcgtggaagc ctgccagaat cagctggtgg atctcatggc gtagcggcat acggtgaccc
    11221 atctcagcgg cgtaaatacg cagctcgaaa atctgaatcc cctgttgcag atccaccagg
    11281 aagacttccg gtgccgggtt gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg
    11341 atttccgtca cttcttcact gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc
    11401 gtgaccgagt cagagagcga ccagttgata aactgctcgg taataaacgc cttgttcggc
    11461 acgattatct ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc
    11521 acgctaccag taagatcgcg gatcgttacc gtatcgccaa tgtgaatcgg tttttcgaac
    11581 aggatgatca ggccagagat aaagttggcg aaaatttcct gcaaaccaaa accgagacca
    11641 acaccgagcg cggcaaccag ccactgcaat ttcgaccact caataccaat cattgagaag
    11701 ccgaccagcc cgccaatcag catcagcaga tattttgtga tggtggtgat ggcgtaaccc
    11761 gtacccggcg ttaaatccag gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg
    11821 cgcaccagct gcgtggtgat gataaatacc agaatggcaa tcagcaccgc accgagggta
    11881 attggctcca gactttctac gccctgtacc gtggaggtga catcccacag cgaaatattt
    11941 tcgaggaagc cgaaagcgga atggatttct gaccacagca caatgaccga aagcagggcg
    12001 atcaacatca aaattgagcg caccagccgc aaggattgcg cactgatggc atcgagatcg
    12061 acttcgcttt catcgacttc aattgctcct tccgggctac tgtgatgatg cgcttcctct
    12121 tcgccacgcg tacgttgcgc taacatctct gcccggcgat gcttcgcccg gtcaaacgcc
    12181 agcctgcgac gctggatcag catccagcgg cggataacgt gataaaccac cagtagcagg
    12241 aaccagatgg caaccgaggt ttcaagcctc gctaacagtg cctgcgccgt tgccagataa
    12301 cccaccgccg acgccagaat ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata
    12361 tggttggtaa tgttgtcgcc gctgccttct ttgttgagat acaacggaat accggctttt
    12421 ttcaggctta gggtgaccac cgccagcgca ccgcaaatga ggataaagca aagccgtccc
    12481 agcgaaccgg aaaattcgcg gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc
    12541 ccgatgctca tcaggtagta gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa
    12601 tgagcgataa acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc
    12661 agcggcacgg tggccgttac accatcgcca atcgcgaccg ccagcggata aggccacgcc
    12721 tcgcgcaagc cgtaacccag cgtcatccac agcaccggta acggtgacgc gacgagaatc
    12781 gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccgactttc
    12841 gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc
    12901 agaatcaacg cgccaaacag cggcaaaatt gtctctttgc tggtcagcat catcacactg
    12961 gctttgccca actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt
    13021 tccagcggcc aggcgatggt catcgggcgc acgtcagagg tccagaacag atagcggtgc
    13081 gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc cgttggagac tttcagcttg
    13141 gtcagttcca atagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga
    13201 gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc
    13261 tgatggattt gccgtagcag cggctgttta ttaagtaaat cctcataacg taaccgttgc
    13321 acacgcaact gcgccatttc ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt
    13381 gccacctgcg cccgcagcgc ttcgccgagc aggttggacg atcccagcca ttgcgactgt
    13441 tcacgcagcg tattcaacgc ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt
    13501 tgcgaggcaa cgagctccat ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg
    13561 ttaattttga attgcgcgac gatatctttc ggcaaatcgg cactgttttc tgccagtaat
    13621 tcggtacttt ccagcgcccg ctccgcctca agctgacgtt ggctattcaa ctgattacgt
    13681 aagccctgca aatacgcatc cagttgctgg ctctcttttt ccgccagctc tgagcgtaag
    13741 cgcgctaatt cctggcggtt attggcagac agctgcgcca gctccagttc atcaacgagc
    13801 gccttaagac gtgcagagtc agactgcaac gcgaaatttt gtgcctgatt gagcggagta
    13861 ttgccggtaa gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt
    13921 tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc
    13981 tgggcctgac ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc
    14041 gcgtcggtag acattcccgg cgacacgctg cgcggctcgt cacgcatgtt gtttaattgt
    14101 gcgcgcagag tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg
    14161 cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg cagactggag cgcctctacg
    14221 acttccggct gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg
    14281 ctatcggggg ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg
    14341 ataatcaggc gcacgtcagc gtttcctttg atggattaga cctggtcttt tttatcgtca
    14401 accaatggcc tggcgtcgtg ttctgcttcg atctcttcag caggaagtgg ggcgggttca
    14461 gcgtctggcg taacaaaggt ttcggtagat actgccagcg gctggccaat tttcgtgaca
    14521 gacaggcttt ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg
    14581 gtggaaccga gtttaaagcg acccatttcc tggcctttca gcagtgccac agaaccgtcg
    14641 ttttccccgg caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg
    14701 cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt
    14761 gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc
    14821 acgttctgag cggtgagatg gttaacggaa aagagatcgc ccggcacgta gatcatctca
    14881 cgcagaatac cgttgcacgg catgtgtacg cgatggtagt cacgcgggga gaggtaagtg
    14941 gtcacaaacg taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct
    15001 tcgaggctgt agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac
    15061 tggctgataa cgccatcggc aggcatgacc agtacattcg gatcggtatc gattgggcgt
    15121 acttcgtcac gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc
    15181 ggcttttgcg cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt
    15241 tttgtcagcc atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt
    15301 ttcggcagaa tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca
    15361 ttgttttgtc gttcctgatc cggccaacat gccggatcct gaaaaaaagg ggacgattct
    15421 aacgacggtt agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc
    15481 tgcgccatgc tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct
    15541 tcctcaaccg cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat
    15601 ttacacagac ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc
    15661 aggtgccaga ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg
    15721 aagtgataca gccgagcggc ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca
    15781 tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg
    15841 acgccagact gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttc
    15901 agcccatcct gagtatggct ggaaaccatc aatacgcgat aaccgatgtt gcggtagata
    15961 tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc
    16021 acgataatcg gctcaatctg caaggtttcg caggccacca ggtaacggtc gataatattg
    16081 agcgacagct ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggctatg
    16141 ggtttcacgc cgtcgtagaa atccggacgc gttaacaccg aggtgcgctc atgcaccgct
    16201 tccacgatcc ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg
    16261 cggtcgccgg ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca
    16321 tcggcggatt ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc
    16381 tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg
    16441 tgattggcgt tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtact
    16501 gactcctggt cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat
    16561 agtcactgtg aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc
    16621 gatcttgaga tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg
    16681 gtgaccgatg ccaatctgaa tattctggca gaagggccga ccattgcagt acatcagtcc
    16741 gatgaacagc tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg
    16801 gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa
    16861 ttcttaaaac agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggacag
    16921 gaccgtcgtt tcctgtttaa atacatgccg gagctggaag tctacttcca ctaccgttat
    16981 ctcgatgtca gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt
    17041 tttaccaagc aggggacgca tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg
    17101 gcttactacc gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg
    17161 aatccacttg cggcggcgtg attttgacgc taaatcgaac gtcttgctga taatttcagc
    17221 acttgagata aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg
    17281 tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag
    17341 ta
//
LOCUS       DADMFL010000082        17238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.083, whole genome shotgun sequence.
ACCESSION   DADMFL010000082 DADMFL010000000
VERSION     DADMFL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.083"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..170
                     /locus_tag="JNZ27_002779"
     CDS             <1..170
                     /locus_tag="JNZ27_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8665045.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..446)
                     /locus_tag="JNZ27_002780"
     CDS             complement(171..446)
                     /locus_tag="JNZ27_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY8665046.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="JNZ27_002781"
     CDS             complement(467..703)
                     /locus_tag="JNZ27_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY8665047.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNZ27_002782"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNZ27_002782"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY8665048.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNZ27_002783"
                     /pseudo
     CDS             complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNZ27_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNZ27_002784"
                     /pseudo
     CDS             complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNZ27_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6356..6853
                     /locus_tag="JNZ27_002785"
     CDS             6356..6853
                     /locus_tag="JNZ27_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY8665049.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6950..7648
                     /gene="proQ"
                     /locus_tag="JNZ27_002786"
     CDS             6950..7648
                     /gene="proQ"
                     /locus_tag="JNZ27_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY8665050.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7668..9716
                     /gene="prc"
                     /locus_tag="JNZ27_002787"
     CDS             7668..9716
                     /gene="prc"
                     /locus_tag="JNZ27_002787"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY8665051.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9908..10789
                     /gene="htpX"
                     /locus_tag="JNZ27_002788"
     CDS             9908..10789
                     /gene="htpX"
                     /locus_tag="JNZ27_002788"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY8665052.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10835..12208)
                     /locus_tag="JNZ27_002789"
     CDS             complement(10835..12208)
                     /locus_tag="JNZ27_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8665053.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12385..13176
                     /gene="kdgR"
                     /locus_tag="JNZ27_002790"
     CDS             12385..13176
                     /gene="kdgR"
                     /locus_tag="JNZ27_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY8665054.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13319..13558)
                     /locus_tag="JNZ27_002791"
     CDS             complement(13319..13558)
                     /locus_tag="JNZ27_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665055.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13717..13860
                     /gene="mgrB"
                     /locus_tag="JNZ27_002792"
     CDS             13717..13860
                     /gene="mgrB"
                     /locus_tag="JNZ27_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY8665056.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13935..14222
                     /locus_tag="JNZ27_002793"
     CDS             13935..14222
                     /locus_tag="JNZ27_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665057.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14892..15035
                     /locus_tag="JNZ27_002794"
     CDS             14892..15035
                     /locus_tag="JNZ27_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY8665058.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15048..15257
                     /gene="cspE"
                     /locus_tag="JNZ27_002795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15048..15257
                     /gene="cspE"
                     /locus_tag="JNZ27_002795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8665059.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15423..16232
                     /gene="rlmA"
                     /locus_tag="JNZ27_002796"
     CDS             15423..16232
                     /gene="rlmA"
                     /locus_tag="JNZ27_002796"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY8665060.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHIHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNZ27_002797"
     CDS             complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNZ27_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY8665061.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4554 a         4170 c         4346 g         4168 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggcgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgagcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccgccttccg gcgcttcaac aataatgctt aaaccatcta ggtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cctagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gttttattat tactccacgc
     4141 tggctgtggg ccagatcttc atacagacca aaggtatctt ccgcctcggc aggtttcgac
     4201 tcttcaggtg aatcgaaggc aatcgcaccg ttaaccagtg ccgccagact ttccagtttc
     4261 accttcgcgc cactgatact gacgttggca tcaacgccgg aaacgttcca gaaacggcta
     4321 ccttttttca ccagatcggt aaaacgccgc tcgatcagga catcaatcac cacgccttgc
     4381 ttgttgggat tgatggcata gtcgtagact tttcccaccg ggatcttgcg gaaatagacc
     4441 aatgaaccgc tgttcagcga accgagatcg ggggcttgca ggtggatcat cagatcgcca
     4501 ttgtccagcc gatatttcgg ttgggtatcg agcgcgacaa agtgatcctg ctctttacct
     4561 ttacccggca tcatgccgat atagttccca ccgacgaggg cgtccagccc ggagacacct
     4621 gccaacgatg cttttggcgt catcagccag aactgagtct cttcgcgcag cgcatctttc
     4681 atatcggact tgatgctgac cttgacttca atcttacgaa gatcgtcgct gaggctgata
     4741 tcctgcactg ttccgacttc aacgccctga taacgaacag gcgtacggcc cggaacaata
     4801 ccatccgccg agataaagtc gatggtgacg gtattacccc ggtcctgata actgtcccaa
     4861 atcagccaac cggcaatcat tagcgcgatg aaaggcagca gccagaaagg tgagatacgg
     4921 cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg tttcctgact catgtgcatc
     4981 ccaaagtaag cggctgtcca gccattccac agcaagaata gtcaaaatta cggcagcgcc
     5041 aaaataaaac gcagccggtc ccatagtaaa agcgaggatc tgatcgcgat taatcagcga
     5101 catggttaaa gatatgacaa acaggtcgag catcgaccag cgtccaatcc aggtcaccat
     5161 ccccagtaac agaatgcgtg tgcgtaaacc ttgctggcat ttaaaatgaa tgctcaacag
     5221 taaagtgaac atgacgatca ctttagtaaa cggtaccaga atactggcga taaacacgat
     5281 tcccgcaacc gcaatgttgc tacttgccag cgacataatt ccggaaagaa tcgtatcttc
     5341 ctgccggcca ccattcagat aaatgataga aataggcaat aggttggctg gtaacaacaa
     5401 aacgattgac gctaacagcg ccgcccagca tttttgcaaa ctatgacgac gacgcaggcg
     5461 tagcgggata tggcaacgcg ggcagagacc acgctgatca ggatagccgg taaaatggca
     5521 cccaagacag acacgaagtt tctcgtccct acgcgtagcg gggcgctgcg gataatatcg
     5581 ctcccacagc tcctcaacat taagatgtga caacgtcacc gtcgttaaaa tcaccaacgc
     5641 cacaaaagag aacaagccga cacccgcctg gatatgggca taatcctgta ccttatatag
     5701 aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta agtcgctcaa
     5761 gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat aaatatgcta
     5821 tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatt gatcccgtta
     5881 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat gacgttggcg tcaatacgaa
     5941 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcata agcaacagca
     6001 tggtgaaggc cattgccgcc aggcgcgtta gcgaccagtc gcgcccgtcg cgaatttttg
     6061 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
     6121 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
     6181 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
     6241 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
     6301 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt aaataatgaa
     6361 caaagcagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
     6421 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc tcactgacgt
     6481 taactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac catttcaggg
     6541 caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcacag cggttgcccg
     6601 caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg cctgtgatgc
     6661 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
     6721 cgacatcgat agtaccgtct tcgctcgctt tacagacgag gacgagcaag gcttacgtca
     6781 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
     6841 cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta taatgacgcc
     6901 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
     6961 acctaagttg aatagcagta aagaagtaat cgcgtttctc gccgaacgtt ttccccactg
     7021 tttcagtgcg gaaggtgaag cgcgtccgct gaaaattggt atttttcagg atttggtcga
     7081 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
     7141 cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
     7201 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
     7261 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
     7321 tgccgcagct gctggtgaga aagaagacgc accgcgccgc gaacgcaagc cacgtccgac
     7381 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
     7441 agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt ctgacatttc
     7501 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
     7561 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
     7621 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg aacatgtttt
     7681 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
     7741 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtaa
     7801 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
     7861 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaatgtgc
     7921 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
     7981 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgccgttttg
     8041 aacgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
     8101 cttataacct tgagcgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
     8161 agtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctgacagga aaaacggata
     8221 aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt ctggcgcaaa
     8281 ccaacagcga agatgttttc tcgctggcaa tgatggcgtt tgcgcgtgaa atcgatccgc
     8341 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcac
     8401 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
     8461 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
     8521 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
     8581 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
     8641 ggaccaagac ccgtactgta acgttgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
     8701 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attccgggct
     8761 tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa aaacagaatg
     8821 tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgacc gaagcggtat
     8881 cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat aacaacggca
     8941 aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggccca ctggtggtac
     9001 tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
     9061 gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
     9121 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
     9181 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
     9241 caccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
     9301 ataacgcgct gccgtgggat agcatcgatg ccgcgactta tgtgaaatca ggagatttaa
     9361 ctgcctttga accggagctg ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
     9421 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
     9481 tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtctgg
     9541 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag ctggatgatc
     9601 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
     9661 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc aagtaatatc
     9721 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
     9781 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
     9841 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
     9901 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
     9961 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    10021 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    10081 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    10141 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    10201 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    10261 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    10321 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    10381 ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg cgggttttat
    10441 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    10501 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    10561 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    10621 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    10681 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgttcatga ctcacccgcc
    10741 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    10801 tactaaaaag cgcgtcctga tcgacgcgct ttttttatgc cctggctcgt ggctgagtga
    10861 tacgtaaacc actgacacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    10921 cgtgcgtacc attatcacca aactgattta gcatcagcgc caccagcgcc gcgccactac
    10981 tctgacccag tagacgagcc gttcctaaca tgccactggc cccaccgcta cgttcgcgcg
    11041 gagcggaggt aataatggtg tggttattgg gtgactggaa taagccaaat ccagcgccac
    11101 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    11161 aaaaaagccc cgcagccatg atgaacaacc ctaaagcacc cagcaatcct gcatgtacgc
    11221 gttcaatcaa atagcctgcc agcggagcca tcaccatcgt tgctaacggc cacggtgtca
    11281 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    11341 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    11401 acagcgggat acgcagtaaa tccaccggta gcagcggtac gggaagagaa agctggcggc
    11461 gaataaagaa aataccaaca acaaccatta ccaccagttc cgcagcaatt aacgtcagcg
    11521 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    11581 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg ggtggcagaa
    11641 aacgcatcgc cagaagcagg gcgataatac ctaacggtac gttgattaaa aataaccatt
    11701 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    11761 caacaataaa cgagtttatg cccatccctc tccccagaaa acgttgtgga tagatcaggc
    11821 ggataagtgc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    11881 caagggtgag catttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    11941 cgaccagacc gcatttataa atacgtcgat agccaaacat atcgcccaga aacgaaaacg
    12001 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    12061 gtgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    12121 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    12181 gcaggccgtc ggcctgaact tttggcatag gaattttata tctttggtga ataatcaaca
    12241 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    12301 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    12361 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    12421 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    12481 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    12541 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    12601 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    12661 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    12721 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    12781 tattcacgga ttgggcgtcg taatccgctg tacagcaccg cgattggtaa ggtactgctg
    12841 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    12901 accgagcgga ccatcaccag tacagaagcg ttattacccg ttctggacca ggtgcgcgag
    12961 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggtaccg
    13021 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    13081 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    13141 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    13201 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg cgttatatta
    13261 aacgcgcttg ctataagaat attttactca tgagtgagaa tctggttatt tattaccctt
    13321 aaccattatc gaccacgata ttgcttttac gtaacagtgg gcaatctgtt atccccaaga
    13381 aaccactttt agtgtgcaag tattgtaccg tgctggtgcc tctggcagtc agataggtac
    13441 attgcaaacc taatcctgcg gcattctctt tgcttccaat caaaacgcca tatccgctga
    13501 gtaataatcc tatccatacc agtgctatca gcataactgt gcgaatgatg aatcgcatta
    13561 caatctcttc tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcta
    13621 ctcattccga aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta
    13681 gtttaatgac ataaggtagg tgaagcggag attggagtga aaaagtttcg atgggtcgtt
    13741 ctggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc
    13801 gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga
    13861 tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac
    13921 tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg
    13981 ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca
    14041 gttcacggac taacgaacag attgaactgc ttgaggcgtt gctggatcag caaaaacgtc
    14101 aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca gataaaaaga
    14161 ccattgagag tcaaaaatcg gttgatgacg aagatattat tcgcctggtc gccgaacgat
    14221 aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc atcctgatta
    14281 tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt caaataatta
    14341 tcatattatt tatttgcgta acgggatatc agaaaaagat attttcctca ttaatttatt
    14401 aggatgttta catcagattt gtgattaagc gtggtattat ttattagcga aacgtttctc
    14461 tcttgatttt tttgctcact catcaatttt tcttatttta aatttacaat ccctttgggg
    14521 attgacttct ctttagggta attgatagcc gttaactgac tgttttatga gaaaaagtga
    14581 tataactttt tattcattgc atagcagaaa atgtgatatt gcacgcacta tgtaataact
    14641 tctccctctg gcctggaaca actgaactta ttgaactatg ttagaaacta cgccagttta
    14701 agtatctgcc tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca
    14761 taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt
    14821 aaaaatggct tgccataatt aacgttgtat gtgataaaag atttcgggtt aaacgaggta
    14881 cagttctgtt tatgtgtggc attttcagta aagaagtcct gggtaaacac gttgacgttg
    14941 aataccgctt ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat
    15001 ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta
    15061 aaggtcaggt taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg
    15121 gcagcaaaga tgtgttcgta cacttctccg ctatccaggg taatggcttc aaaactctgg
    15181 ctgaaggtca gaacgttgag ttcgaaattc aggacggcca gaaaggtccg gcagctgtta
    15241 acgtaacagc tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct
    15301 ataaagcctc gtcgaatgcg aggcttttta ctatgcttta tcttcgctcc tggcgttcgg
    15361 atatttgcca gccacgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg
    15421 tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata
    15481 tctgtcccca acgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg
    15541 ttcagcataa acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg
    15601 cattcttaga tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgcggg
    15661 aacggcttga tgaaaaggcc acggcggtgc tggatattgg ctgtggtgaa gggtattaca
    15721 cacacgcatt tgccgatgcg ttgcccgaaa tcaccacgtt tggcctggat gtctcgaagg
    15781 tagcgataaa agcggcggcg aaacgctatc cgcaggtcac tctttgtgtc gcttccagcc
    15841 accgtttgcc gttttccgat accagtatgg acgccataat acgtatttac gcgccgtgta
    15901 aagcagaaga attagcacga gtagtgaagc ccggcggctg ggtcattact gccacgccgg
    15961 gaccgcgaca tttgatggag ctgaaggggc tgatttacaa tgaagtacat attcatgcac
    16021 ctcatgcaga acaactggaa ggttttacat tacagcagag tgcggagttg tgttatccga
    16081 tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat gacgccgttt gcctggcgtg
    16141 cgaagccaga agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact
    16201 ttaatattca cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga
    16261 cgccgatgcc gatcagcacc agcccgccga gaatttccgc ttttttccca ataattgagc
    16321 cgataaagcg accaaccatc atccctaatg tcgacataat caaggttgca caaccaatgg
    16381 ccaatgcggt cgcgataatg ttgacctgaa ggaaagcaag accaacaccc acagccatgg
    16441 catccaggct ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgc cgacggcgcg
    16501 gctcttcatc ttcatcatct gagccacgaa aaccctcaat aatcattcgc ccgccgagga
    16561 atatcagcag cacaaacgca atccagtgat tccattcaag gacaaaccgg ctggctaaca
    16621 tgcccattcc ccagccgatc agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg
    16681 ttcgcaatgc ttcagaaaat ttcggtttat ggaggggggc acctttaccg attgatgcag
    16741 caaatgcatc catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa
    16801 cgtcctgacc ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa
    16861 caacaagccg cgatgggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc
    16921 tcgcctgact gaaagtcttc agtccgtacg tgccacgttt tgcaacgagt atgttgacac
    16981 gtacatttcc ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat
    17041 tttgagaata taaaacaact gttggaatat attatttcat tgctttattg ataacgattt
    17101 tcatttatgt ttatatatca atgaaacgtt aaaataaata acaaataagg gaagagataa
    17161 tatagcctaa gctatgtctg attattaaaa atacactaaa catagctttg gctaaattca
    17221 taagttattg agttgata
//
LOCUS       DADMFL010000083        17183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.084, whole genome shotgun sequence.
ACCESSION   DADMFL010000083 DADMFL010000000
VERSION     DADMFL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.084"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(329..673)
                     /locus_tag="JNZ27_002798"
     CDS             complement(329..673)
                     /locus_tag="JNZ27_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8665062.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JNZ27_002799"
     CDS             805..2715
                     /locus_tag="JNZ27_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY8665063.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JNZ27_002800"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JNZ27_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY8665064.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JNZ27_002801"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JNZ27_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY8665065.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JNZ27_002802"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JNZ27_002802"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY8665066.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JNZ27_002803"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JNZ27_002803"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY8665067.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNZ27_002804"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNZ27_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY8665068.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JNZ27_002805"
     CDS             complement(8251..8853)
                     /locus_tag="JNZ27_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY8665069.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKQSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..9761)
                     /locus_tag="JNZ27_002806"
     CDS             complement(9210..9761)
                     /locus_tag="JNZ27_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8665070.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="JNZ27_002807"
     CDS             9987..10214
                     /locus_tag="JNZ27_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8665071.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="JNZ27_002808"
     CDS             10208..10639
                     /locus_tag="JNZ27_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665072.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNZ27_002809"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNZ27_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY8665073.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNZ27_002810"
     CDS             complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNZ27_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY8665074.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNZ27_002811"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNZ27_002811"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY8665075.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="JNZ27_002812"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="JNZ27_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY8665076.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="JNZ27_002813"
     CDS             complement(12383..12730)
                     /locus_tag="JNZ27_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY8665077.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNZ27_002814"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNZ27_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /locus_tag="JNZ27_002815"
     CDS             14063..14884
                     /locus_tag="JNZ27_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8665078.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="JNZ27_002816"
     CDS             complement(14841..15287)
                     /locus_tag="JNZ27_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY8665079.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPSSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /locus_tag="JNZ27_002817"
     CDS             15457..15561
                     /locus_tag="JNZ27_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665080.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNZ27_002818"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNZ27_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY8665081.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..17166)
                     /locus_tag="JNZ27_002819"
     CDS             complement(16108..17166)
                     /locus_tag="JNZ27_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8665082.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
BASE COUNT         4408 a         4175 c         4356 g         4244 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgctgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtatt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggcttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg acggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgttctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cag
//
LOCUS       DADMFL010000084        16931 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.085, whole genome shotgun sequence.
ACCESSION   DADMFL010000084 DADMFL010000000
VERSION     DADMFL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.085"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..882)
                     /locus_tag="JNZ27_002820"
     CDS             complement(<1..882)
                     /locus_tag="JNZ27_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8665083.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="JNZ27_002821"
     CDS             989..1285
                     /locus_tag="JNZ27_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8665084.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNZ27_002822"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNZ27_002822"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY8665085.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNZ27_002823"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNZ27_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY8665086.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNZ27_002824"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNZ27_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY8665087.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="JNZ27_002825"
     CDS             4297..4380
                     /locus_tag="JNZ27_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY8665088.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNZ27_002826"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNZ27_002826"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY8665089.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /locus_tag="JNZ27_002827"
     CDS             complement(5858..6700)
                     /locus_tag="JNZ27_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY8665090.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="JNZ27_002828"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="JNZ27_002828"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY8665091.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNZ27_002829"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNZ27_002829"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY8665092.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNZ27_002830"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNZ27_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNZ27_002831"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNZ27_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY8665093.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQILVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="JNZ27_002832"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="JNZ27_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY8665094.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNZ27_002833"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNZ27_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY8665095.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="JNZ27_002834"
     CDS             14491..15690
                     /locus_tag="JNZ27_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8665096.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4307 a         4224 c         4371 g         4029 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccaacgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaagcagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacaccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgagattg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaatctggcc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctggctac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaaa tttaaaatca ccccctctgg attgaatcaa
    15961 cttgctctat ttaagacgat tgtttttaca ttttcaccac ctgtatcagg tggtgttttt
    16021 ttattggcgg caatgccttt atcttatttt tattacgttg taaatatggc tattcctaaa
    16081 tgaaatttga tttgctcagt ttatttgtga tgtcagggga gtgcttgcca tgagagtcgt
    16141 gatattatta ttcatcattt ttataccgag tgtggcggat gctttttcat tcgatagttc
    16201 aataattaat ctcaaatgtc ctaggcgtgg aactatcgaa ataatattac acagatacga
    16261 acacacccag gaaagctggg gaggggagaa ttttgaaacc gggagtgggt atagtcatga
    16321 gggcgcgtta tttatttttg actttgccaa tcaggataaa atgatttaca atcgcgcgga
    16381 gaattcgttt gctttttggt atgaatcttc ccggaaatta gttaactgta acttaataac
    16441 gcttaaaagc acatatcctg tcaacctgcc ttatgttcat ggctgatgtg atgtgtgtcg
    16501 ggaactaaca tcttcatctt tgtatatgac attgcgataa tgttaagctg gtctttgtca
    16561 taaatttacc cttagagata ctactccctt ctgtcaatat tattcaagcc gaacgttata
    16621 ctggcttttg tcggtcacgg atatcttttt ttcagtgaga attcaaagtg agggtatata
    16681 agaagtcgta tgagtaagta tctttgttgt ggtgggttat tattttatgg gcaaaatatt
    16741 aagcagcgaa atcttttttt tgcccagacg ttttttgtag aaaaaagaat ttattagtcc
    16801 ggctgaaaag caagaactgc tataactaat taagcgtaac ggaacggtac aaaacatgcc
    16861 cttacaacaa ttttcccgat tagcgctcac tctggcggta atgctgccaa cttactgatt
    16921 tagtgtatga t
//
LOCUS       DADMFL010000085        16837 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.086, whole genome shotgun sequence.
ACCESSION   DADMFL010000085 DADMFL010000000
VERSION     DADMFL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.086"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNZ27_002835"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNZ27_002835"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY8665097.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JNZ27_002836"
     CDS             1020..1703
                     /locus_tag="JNZ27_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY8665098.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JNZ27_002837"
     CDS             1700..2161
                     /locus_tag="JNZ27_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665099.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JNZ27_002838"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JNZ27_002838"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8665100.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JNZ27_002839"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JNZ27_002839"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY8665101.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNZ27_002840"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNZ27_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY8665102.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JNZ27_002841"
     CDS             complement(4704..4787)
                     /locus_tag="JNZ27_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY8665103.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JNZ27_002842"
     CDS             5380..6210
                     /locus_tag="JNZ27_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8665104.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNZ27_002843"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNZ27_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY8665105.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="JNZ27_002844"
     CDS             complement(7339..8799)
                     /locus_tag="JNZ27_002844"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY8665106.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNZ27_002845"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNZ27_002845"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY8665107.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="JNZ27_002846"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="JNZ27_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY8665108.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNZ27_002847"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNZ27_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY8665109.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNZ27_002848"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNZ27_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY8665110.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNZ27_002849"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNZ27_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY8665111.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..16612)
                     /gene="fimD"
                     /locus_tag="JNZ27_002850"
     CDS             complement(13976..16612)
                     /gene="fimD"
                     /locus_tag="JNZ27_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY8665112.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16679..>16837)
                     /locus_tag="JNZ27_002851"
     CDS             complement(16679..>16837)
                     /locus_tag="JNZ27_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HAY8665113.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     SKMTGVME"
BASE COUNT         4089 a         4338 c         4099 g         4311 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcatttttcc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact atttccgctg aaattataat tgagcaatcc ggcattaata ccgggatccc
    16081 ataactcagg aggaatataa ccacgcgcgc gattactcat aaatgcctga gggatcgtca
    16141 ggttcagtcg ctgctgacca acatccagat gcgcagtagc gtcctggacc attgtggtta
    16201 atggcacaca ggcatcatcc gccagcagat tcataccggc gacagaagcc gtattcagcc
    16261 ccatactggc gagttgcgcg cgtgtcaggc agggaacaat cccttgttca ctgtcgcccg
    16321 tattaaatgt gacatcacgc gttgccatat aaccattatt caaatagata tcgacgcgat
    16381 acgtccctgg cggtaattct tgcccatttt caaaacgcga taaatcggcc acagcctggg
    16441 gatcatccgc taaaaagcgc agattaaaat agaggtcggc agatgacaaa ggtgcctgtg
    16501 cggcaaaagc acaggcgaca acgagtcgga caaaaaaacc agccaaacga tgcttacgaa
    16561 tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt cagatatgac attattcctc
    16621 cggtttaaaa tcacccggca gtcaattctt tttattcagg cgaaaaattc cccctgcgtt
    16681 attccattac gcccgtcatt ttggaggtaa gtgcgccata atcatttatt gttcggtaag
    16741 taatattgct tcctgcatca gaaggcaatt taaccgtgct ttcgcccatt ggaggcacca
    16801 atgcattttc aagaacccgg gttccggcat tcaactc
//
LOCUS       DADMFL010000086        16755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.087, whole genome shotgun sequence.
ACCESSION   DADMFL010000086 DADMFL010000000
VERSION     DADMFL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.087"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JNZ27_002852"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JNZ27_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8665114.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTMSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVHNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JNZ27_002853"
     CDS             2219..2767
                     /locus_tag="JNZ27_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY8665115.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JNZ27_002854"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JNZ27_002854"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY8665116.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JNZ27_002855"
     CDS             complement(3664..4854)
                     /locus_tag="JNZ27_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY8665117.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JNZ27_002856"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JNZ27_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY8665118.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="JNZ27_002857"
     CDS             complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="JNZ27_002857"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY8665119.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JNZ27_002858"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JNZ27_002858"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY8665120.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12382
                     /gene="pepN"
                     /locus_tag="JNZ27_002859"
     CDS             9767..12382
                     /gene="pepN"
                     /locus_tag="JNZ27_002859"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY8665121.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JNZ27_002860"
     CDS             complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JNZ27_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8665122.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13353..14000)
                     /locus_tag="JNZ27_002861"
     CDS             complement(13353..14000)
                     /locus_tag="JNZ27_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8665123.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JNZ27_002862"
     CDS             complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JNZ27_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY8665124.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15154..16113)
                     /locus_tag="JNZ27_002863"
     CDS             complement(15154..16113)
                     /locus_tag="JNZ27_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8665125.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JNZ27_002864"
     CDS             complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JNZ27_002864"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY8665126.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4186 a         4323 c         4288 g         3958 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgctctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaaa tatgcaacca aaagtaaaaa ttaaggagct ttgtgaacac
     6361 cgtcatattt ccatagaaac gtgatgatat ttacagcaat ttttatctat ttatatgatt
     6421 tacttatatt taaattaact aaacggaaat tttgtttctg atggaaactt tatcgacctg
     6481 gcacaaaatc ctctttcaga catccagaat gccaaaaaat agtatgaaaa ttgtgctatc
     6541 agctaagaaa aaactaatcc gcaataagaa tgcgggttat tttttgtgcg taaatgttgc
     6601 aatgtaatat tccgtaacag gatgatcgtt atggcggggg tgcaaataga ttgcttgact
     6661 taacaaacag ataacttgac agaaaggata aaaaagagcc agcaggcgct ggctctttga
     6721 ggaagaaaat cagaagctgg cgttgcgcgg agtacgtggg aacggaatca catcacgcac
     6781 gttttgcacg ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg
     6841 cggaacagta ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc
     6901 catttccagc atacgctcgt ccagaacgtc cagacgttct tcacgctggg agccaccaat
     6961 gatctcaccg atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt
     7021 gaggcgcata tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt
     7081 aaagtgttct tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac
     7141 cgggttttca aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac
     7201 ctgtgcgaaa tcggcttcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg
     7261 ttcagcgaag aatttcatgt cgtctgcgcg ttcttcgaga accgctttga agacatattt
     7321 cagcatggct tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc
     7381 cagcatccag aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg
     7441 gccgaaggta taaattttgg ataatgcgca agcgtaggtt tcgccgttca actggccaga
     7501 tacggtcagg aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc
     7561 gttacgcggc aggttttcca gatccagcgt agaaacgcgg aacatttcgc ctgcaccttc
     7621 ggtatcagat gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa
     7681 gcgatgcagc gcctgcgcca gcgtatggcg aacgcgcgcg acggcaccaa tcaggtttgt
     7741 gcgcggacgc aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat
     7801 cgggtaagtg tctggatctt caacccaacc agcaacttca accttgctgg cctgaatttc
     7861 aaattgttgc ccctggcccg gcgatgccac gactttaccc gtcacaatga ccgagcagcc
     7921 ggtggtcaga cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg
     7981 tacaggatca aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc
     8041 tcggcgggta cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg
     8101 gagtacgtcg gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa
     8161 taaacacttg tccacccgta aatggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgaacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag
    10021 taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac
    10081 ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg
    10141 tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa
    10201 aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca
    10261 aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg
    10321 ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg
    10381 ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc
    10441 gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga
    10501 gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga
    10561 gaataagggt ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac
    10621 cgacaaagat tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac
    10681 cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt
    10741 cttccgcgat caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa
    10801 tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat
    10861 ccgcccggac atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg
    10921 cgcggaagtg atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat
    10981 gcaactctat ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc
    11041 gatggaagat gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg
    11101 tacgccggtt gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac
    11161 catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc
    11221 gtttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca
    11281 tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt
    11341 ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata
    11401 taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg
    11461 ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca
    11521 gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct
    11581 cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga
    11641 aatggctgaa ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact
    11701 cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta
    11761 ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg
    11821 cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata
    11881 ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca
    11941 gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct
    12001 ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac
    12061 ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc
    12121 gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg
    12181 ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc
    12241 acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat
    12301 gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa
    12361 gataactaaa gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc
    12421 gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc
    12481 acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg
    12541 cctacaagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg
    12601 ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag
    12661 ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag
    12721 actaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac
    12781 atcatgcgtc accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc
    12841 ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa
    12901 acccggtcga tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag
    12961 tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg cccgacgcgc
    13021 ggcatcgcgc cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg
    13081 cagcagacgc gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt
    13141 ggtgcccgct aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggcgcagcag
    13201 ggtactttta ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg
    13261 taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa
    13321 caatggtgtg ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa
    13381 gccgggttcc agctcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg
    13441 ccgagcaggg cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc
    13501 gcattcatcg ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc
    13561 gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg
    13621 ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc
    13681 accagcccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc
    13741 gtaccgagcg ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc
    13801 agcgggatca gcgccagatg ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc
    13861 gtcaccactc cttccggtga aggcaaaata cgcgtcgaca gccagccaac cgaggaggcc
    13921 agttgccaca ccgccacgat gcccaccggt aaaaaccagg gggcaacgcg caataaccac
    13981 ttcttcactg gcgttgccat catcgtgttc cttagctttg cgcgacttta cgggggataa
    14041 aatcattcgc caccgcttcg ccttgcggat tcagcggctg cggctgggga atttccggaa
    14101 tggcgacatc cagatgtggg aacagcaact cgccaacccg atacgcttct tccagatgcg
    14161 ggtagcccga aagcacaaag ctgtcgatgc caagcgcggc gtattcgttg attcgcgcgg
    14221 cgaccgtagg accatcgccc accagcgccg ttccggcacc gccgcgcact aagccaacgc
    14281 ccgcccataa attggggctg atctccagat tgtcgcgctt gccgttatgt agcgccgcca
    14341 ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc ttgtgctttg gcgatagtct
    14401 catcatcaag atgcgagatt aaccgctcgg cggcctgcca cgcttcgtcg ttagtttcac
    14461 gaacaatcac atgcagacga ataccgaaac gaattttgcg cccatacgcg gcagctttcg
    14521 cccgcacttg ttcgattttc tctttaacaa gttccggcgg ttcgccccag gtgaggtaga
    14581 gatcaacttg ttctgccgcc agctcctggg cgacatctga cgatccgcca aagtaaagcg
    14641 gaggataagg ctgttgaatc ggcgggaaga gcagttttgc tccgcgcaca tgaatatgtt
    14701 taccgttgaa attgacggtt tcccccagca ataagcgccg ccagacctgg gtaaattccg
    14761 ccgaggcttc gtagtgctcg ctatgatcaa ggaacacgcc gtcgcctgcc agctcttgtg
    14821 gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt tgagagacgg tcaagcgtgg
    14881 cggcctggcg ggcggcaaca gtaggtgagg ttacgctggg acgcagggcg acaagaaact
    14941 tcagccgctg cgtcaccggg atcatcgacg ctgcaaccag ccacgcatct tcacacgatc
    15001 gccccgttgg gatcagcaca ccggtatagc ccagacgatc cgccgcttgc gcaatttgtt
    15061 gcagataacc gtgatcaacc gggcgtgaac cttcttccgt tcccagatag tgcccgtcac
    15121 cgtgggtcgg taaaaaccag aacatattca gactcataat tgttttcctt ccagttgagt
    15181 gggctgccag atgcgctggc gaatatcgac ttttttcggc accagacggt tttcataaaa
    15241 cagatctgcc gtttgctgct gtaaggcggc aacctcggcg ttaaccggtt tgatggtggt
    15301 aggagggcga tgatctaagt aagaggcaat caccggtgcc ggtaagccca tcgtttttgc
    15361 cagtaaagcg atgctttgtt cgcgctggct gcgggttaac gcatcggcct cactaaaggt
    15421 tgccagtacg ccctgaataa aagcgccgtt tttttctgca tagggacgag ctgccagata
    15481 aaacgatcca gtttgattga gatcggtgcc gtctttcagc acccgcacgc cgccctgtaa
    15541 taacgcagcg gagtagtagg gatcccagat agcccaggcg tcaacgttac cttgctggaa
    15601 cgcggcgcgg gcaccggcag gcgtcaggta agtgggctgg atatcggtga atttaagtcc
    15661 ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg gaacctttct gaaaggcaac
    15721 tttgtgacct ttaagatcgg ctacggtttt gatcgggctg ttttctgcca ccagaatcac
    15781 ttcggctttg ggcttcggtg gctcgacgcc cacatatacc aaatcagccc cggcagcctg
    15841 ggcaaagatt ggcggaatat ccccggtact gccgagatca atactgccaa cgtttaacgc
    15901 ttccaacatt tgcggacccg cggggaactc cacccaggag atttttgatt gcggatagcg
    15961 tttttccagt aactggtggc tttttgccag caccatacca atactgcctt tctgatagcc
    16021 tatacgtaac gcttcaggcg aggattctgc agcaaccgca aaagtagaga cgctaagcaa
    16081 tcccgccagc gccagtttaa tgatgttacg catgggcatt acctcgcaga gacagaaggt
    16141 caggaacctg aacatcgcgg cggtgcaatg cctgccagaa agtttctagc gcggtatcaa
    16201 gatggggttg cagatttggc gtgaactggg gtctgtgatg gtaatcaatt aattgtgagt
    16261 catcggcaaa cacgccgtgc aggatctcct gagctttcag tgcgcttaaa actggtttaa
    16321 gggcataatc gaccgccagc agatgggcca cggtaccgcc cgtcgccagc ggtagcacca
    16381 ctttgccttg caaagcgcgc tctggcagca ggtcgagcag ggttttcagc gcgccggaat
    16441 aggcggcttt atacacaggc gtggcgacaa tcagcccatc agcctgttgc agctgttcgg
    16501 tgaaggtctt gagtgccgga ctatcgaaac gagcataaag cagatcttcc ggggcgaagt
    16561 tttgcagatt ccagtgataa acctctacat ctaggccatt tagtttttcc cgcgcatatt
    16621 ccagcaagga gctggagcga gaaggaaagc gaggactacc cgccagggtg atgacacgca
    16681 tactctctcc ttataaccaa ttgttctttt ttttgttaac attgataaca attcggtcag
    16741 tctgtcggag agaca
//
LOCUS       DADMFL010000087        16603 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.088, whole genome shotgun sequence.
ACCESSION   DADMFL010000087 DADMFL010000000
VERSION     DADMFL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.088"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            9..746
                     /locus_tag="JNZ27_002865"
     CDS             9..746
                     /locus_tag="JNZ27_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8665127.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JNZ27_002866"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JNZ27_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY8665128.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JNZ27_002867"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JNZ27_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY8665129.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JNZ27_002868"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JNZ27_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY8665130.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..6706
                     /locus_tag="JNZ27_002869"
                     /pseudo
     CDS             5041..6706
                     /locus_tag="JNZ27_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            6758..7913
                     /locus_tag="JNZ27_002870"
                     /pseudo
     CDS             6758..7913
                     /locus_tag="JNZ27_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(7879..8001)
                     /locus_tag="JNZ27_002871"
     CDS             complement(7879..8001)
                     /locus_tag="JNZ27_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665131.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8204..9265
                     /locus_tag="JNZ27_002872"
     CDS             8204..9265
                     /locus_tag="JNZ27_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY8665132.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLINKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTE"
     gene            9359..9889
                     /locus_tag="JNZ27_002873"
                     /pseudo
     CDS             9359..9889
                     /locus_tag="JNZ27_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            9927..12299
                     /locus_tag="JNZ27_002874"
     CDS             9927..12299
                     /locus_tag="JNZ27_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY8665133.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12344..15139
                     /locus_tag="JNZ27_002875"
     CDS             12344..15139
                     /locus_tag="JNZ27_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8665134.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            15501..>16603
                     /locus_tag="JNZ27_002876"
     CDS             15501..>16603
                     /locus_tag="JNZ27_002876"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8665135.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT         4797 a         3670 c         3675 g         4461 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtaata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caattagaag aggaacattt
     5041 atgaagtctg cattaaagaa acgtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaaa ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg
     5461 accggtcgag ctcccgcccg cgttggtgtc tattccaata ccgatgctca ggatggtatt
     5521 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
     5581 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg
     5641 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt
     5701 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
     5761 ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
     5821 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
     5881 gcttataatg ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa
     5941 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
     6001 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     6061 ctctttacct ccgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
     6121 acctatcctg gcggtactca ccaccccaat gtttatgtgg tggaaaggaa aacttcaacc
     6181 cggtaattat gacaagctga tttccgcaat ggatttctac ccaacagctc ttgatgcagc
     6241 cgatatcagc attccaaaag accttaagct ggatggtgtt tccttgctgc cctggttgca
     6301 agataagaaa caaggcgagc cacataaaaa tctgacctgg ataacctctt attctcactg
     6361 gtttgatgag gaaaatattc cattctggga taattaccac aaatttgttc gccatcagtc
     6421 agacgattac ccgcataacc ccaacactga ggacttaagc caattctctt atacggtgag
     6481 aaataacgat tattcgcttg tctatacagt agaaaacaat cagttaggtc tctacaaact
     6541 gacggatcta cagcaaaaag ataaccttgc cgccgccaat ccgcaggtcg ttaaagagat
     6601 gcaaggcgtg gtaagagagt ttatcgacag cagccagcca ccgcttagcg aggtaaatca
     6661 ggagaagttt aacaatatca agaaagcact aagcgaagcg aaataactaa accttcatgc
     6721 ggcggatttt tccgccgcct tattgagcga gatagcaatg cacgttacag ccaagccctc
     6781 cagttttcaa tgtaatctca aatgtgatta ctgtttttac cttgaaaaag agtcgcagtt
     6841 tactcatgaa aaatggatgg atgacagcac tctgaaagag ttcatcaaac aatatatcga
     6901 agcgtctggc aatcaggtct attttacctg gcaaggcggt gaacccactc tggctggcct
     6961 ggattttttc cgtaaagtta ttcactatca acaacgctat gcaggccaaa aacgtatttt
     7021 taatgcatta caaacgaatg gcattttatt gaataatgaa tggtgtgcct ttctcaaaga
     7081 acatgatttt ctggttggta tctcgatcga tggcccccag gagttacatg accgttacag
     7141 acgcagtaat tcaggtaacg gtacttttgc aaaagtgata gcagccatcg agcgtctgaa
     7201 atcatatcaa gtagagttta atacgttaac cgtcattaat aacgttaatg tccattaccc
     7261 tcttgaggtt tatcattttt taaaatctat cggcagtaaa catatgcaat ttatcgaatt
     7321 gctagaaacc gggacgccga atattgattt cagtggtcat agtgagaaca cattccgtat
     7381 cattgatttt tctgtgcctc ccacggctta tggcaagttt atgtcaacca tttttatgca
     7441 atgggttaaa aacgatgtgg gtgaaatttt cattcgtcag tttgaaagct ttgtcagccg
     7501 ttttttgggg aatgggcata caagttgtat tttccaggag tcctgcaagg ataatctggt
     7561 tgttgaaagt aatggagaca tttacgaatg cgaccatttt gtctatccac agtacaaaat
     7621 tggaaacatt aataaatctg aactcaaaac gatgaacagt gtacaactga cagcgcaaaa
     7681 aacggatttc agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgtc
     7741 ctaagcatcg tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata
     7801 aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggttgagtta gctaagaaca
     7861 gagtaccgct ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct
     7921 ctcttttaac tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat
     7981 tcctgtcggg tgtttcgtca tcatcatctt cccttgcgaa aatcctggct tacgtggtat
     8041 gcgtcagggc attagcaata cccttgatta tcaatagcat aaccataacc actgaaaatt
     8101 aaaaatttct atatatttca tattattaaa tgatacttaa ataaatttca tcagtaaatc
     8161 attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat
     8221 tacgttttgt atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa
     8281 gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt
     8341 taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa
     8401 agaaaccgat aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt
     8461 attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg
     8521 gttaacagat tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat
     8581 ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt
     8641 actgatcaac aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat
     8701 cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac
     8761 agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac
     8821 tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag
     8881 cgaagcgaca tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct
     8941 aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga
     9001 gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc
     9061 gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat
     9121 caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag
     9181 ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta
     9241 tgagttccat caactcactg aatagcgccc tacgaataag cctaaaaatt gccaacatgc
     9301 ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt
     9361 gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa
     9421 aaccacactg cttaaaacat tatcccactg ctggccgtga tttaaaggtg atatttcttc
     9481 tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga
     9541 gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca
     9601 tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat
     9661 tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa
     9721 atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact
     9781 gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg
     9841 tgtctggaac ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa
     9901 gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc gtcattttat
     9961 gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag
    10021 agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg
    10081 aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc
    10141 ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg
    10201 agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta
    10261 gtgcaactaa taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta
    10321 gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg
    10381 acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca
    10441 aacgcttcaa cgctgatgat agcaaggtga aattgggtta tcgcactacg cgtttggact
    10501 ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa
    10561 gtacctattt ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggagc
    10621 tggctgataa ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg
    10681 cggattatgt ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg
    10741 atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct
    10801 taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcaa tctgatcgcg
    10861 aaatgggtaa taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca
    10921 aactacgtac caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca
    10981 tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg
    11041 gattaggaca catttcccag gctgtagata attacacctt caaaacacgc ctggactggc
    11101 aaaaattcgc cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt
    11161 ccgatgcatg gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa
    11221 agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca
    11281 cgctgtatat ggcggatcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt
    11341 atgactatga caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg
    11401 atatttttgc tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga
    11461 atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta
    11521 ataaaaccct gacgcgttat cagaatttga aaacgcctta taacgatgaa ctggcaatgg
    11581 gattgcagca aaaaatcgat aagaacgtta ttgcacgcgc aaactatgtt taccgtgaag
    11641 cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg
    11701 aatataacaa cgatggcaaa accaaaacgc attcgtttaa cctcagtttt gaactggccg
    11761 aacccctgca tatccgccag gtagatatta acccgcaaat tgtctttagc tatatcaaga
    11821 gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg
    11881 tggtttataa cggtaatctg gtctcttacg atagcgttcc agtggcagat tttaataacc
    11941 cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga
    12001 atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc
    12061 aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc
    12121 tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga
    12181 tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca acgaacaccg
    12241 gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat
    12301 agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag
    12361 aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc
    12421 cgccgcctta cagcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata
    12481 tatgatttat ccgcatgctc atccaaagga tcaggtaaat ttatggctgc aaattcatac
    12541 cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat
    12601 gtttaacggc gcaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg
    12661 cctgcgtttt ggtctcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt
    12721 gagtttgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg
    12781 gagtaatgcc gcaacctttg aaaaactcga agtagacgct gaacgtggcg taatttctga
    12841 ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct
    12901 gctggctaat acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac
    12961 ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac
    13021 ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt
    13081 aagtaagttt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa
    13141 ccacctgcgc tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta
    13201 ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg
    13261 gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt
    13321 gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct
    13381 gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc
    13441 agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc
    13501 tacccgcctc acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat
    13561 gctgaccagt cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga
    13621 gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa
    13681 atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct
    13741 tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa
    13801 caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga
    13861 cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact
    13921 ttccaatggt gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat
    13981 tattgccgta tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc
    14041 gctggcaaat aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa
    14101 acgctggagt gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt
    14161 gctctccgtt agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg
    14221 aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca
    14281 ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac
    14341 gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc
    14401 cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt
    14461 cattgtcggt aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc
    14521 aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa
    14581 cgcatcggtt actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg
    14641 ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt
    14701 cgcactggca aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt
    14761 ttcttctcgc ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac
    14821 ttgtcaacca gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg
    14881 tttgactaag gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag
    14941 cctcgatatc caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat
    15001 tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacgat
    15061 agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata cttataccgg
    15121 agtattattg ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
    15181 tattcgctat ttttatgtaa taattttata aatgcgttca aaataataac caagtactaa
    15241 tagtgatatt ttaatgtctg atttttacgt gataattcag gaggcacaga aagcgcataa
    15301 aaataacaac ataaaacacc ttgccaccac ccaagaattc tctattgcat tgtttttaat
    15361 gaaaaaaata tcattccaac aatatctcac gataaataac attaggattt tgttatttaa
    15421 acacgagtcc tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca
    15481 atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac
    15541 tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc
    15601 tgcacgaaat gcgcgacgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg
    15661 gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccaca
    15721 agttgatgga tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg
    15781 cagccatcga cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg cctgcgccga
    15841 aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt atgctcggcg
    15901 ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata
    15961 aaccaaacct ggtgtgcggt ccggtgcaaa tctgctggca taaattcgcc cgctactggg
    16021 atgtggagct gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca
    16081 tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc gactttcggc gtgacctaca
    16141 ccggtaacta tgagttcccg caaccgctgc acgatgcact ggataaattc caggccgaca
    16201 ccggtatcga catcgacatg cacatcgacg ccgccagcgg tggctttctg gcaccgttcg
    16261 tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag
    16321 gccataaatt cggtctggct ccgctgggct gcggctgggt tatctggcgt gacgaagaag
    16381 cgctgccgca ggaactggtg ttcaacgttg actacctcgg tggtcagatt ggtacttttg
    16441 ccatcaactt ctcccgcccg gcgggtcagg tgattgcaca gtactatgaa ttcctgcgcc
    16501 ttggtcgtga aggctatacc aaagtacaga atgcttccta ccaggtcgct gcctatctgg
    16561 cggatgagat cgccaaactg gggccgtatg agttcatctg tac
//
LOCUS       DADMFL010000088        16323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.089, whole genome shotgun sequence.
ACCESSION   DADMFL010000088 DADMFL010000000
VERSION     DADMFL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.089"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="JNZ27_002877"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="JNZ27_002877"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY8665136.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="JNZ27_002878"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="JNZ27_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY8665137.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="JNZ27_002879"
     CDS             2339..2554
                     /locus_tag="JNZ27_002879"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665138.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="JNZ27_002880"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="JNZ27_002880"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY8665139.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /locus_tag="JNZ27_002881"
     CDS             4449..5843
                     /locus_tag="JNZ27_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY8665140.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="JNZ27_002882"
     CDS             5920..6417
                     /locus_tag="JNZ27_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY8665141.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQFVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="JNZ27_002883"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="JNZ27_002883"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY8665142.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="JNZ27_002884"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="JNZ27_002884"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY8665143.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="JNZ27_002885"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="JNZ27_002885"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY8665144.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9030..9665
                     /locus_tag="JNZ27_002886"
     CDS             9030..9665
                     /locus_tag="JNZ27_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY8665145.1"
                     /translation="MTAPFFPRILAAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="JNZ27_002887"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="JNZ27_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY8665146.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="JNZ27_002888"
     CDS             complement(10257..11237)
                     /locus_tag="JNZ27_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY8665147.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="JNZ27_002889"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="JNZ27_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY8665148.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="JNZ27_002890"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="JNZ27_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY8665149.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="JNZ27_002891"
     CDS             12540..12830
                     /locus_tag="JNZ27_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY8665150.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="JNZ27_002892"
     CDS             12838..13431
                     /locus_tag="JNZ27_002892"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY8665151.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="JNZ27_002893"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="JNZ27_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY8665152.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14874..15837
                     /locus_tag="JNZ27_002894"
                     /pseudo
     CDS             14874..15837
                     /locus_tag="JNZ27_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15882..>16323
                     /locus_tag="JNZ27_002895"
     CDS             15882..>16323
                     /locus_tag="JNZ27_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY8665153.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFY"
BASE COUNT         4038 a         4205 c         4110 g         3970 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tgcgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag agcattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcta catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaaa aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc agggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggtc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggtctg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatcca gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaatt gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca tctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcacttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata accatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atagccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat tcgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcactcg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 ccgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccacctgc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggcgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgatcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgcc
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta taccatggtg ctgttattac gcatctggat gcagtgggct
    12061 cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgtcat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgttc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gaccatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactaa tcattcattg
    14701 attataaata tgtacaacct ggcgcacaaa tttaatattt aatttctgac tttatttcaa
    14761 gttgtgataa acatagacaa gttgtcagac aggttaacca atttgtcgca caaagcactg
    14821 ataagcacaa ttagatatca accaatttcc atccttcaac agggagtttt tttatgaaaa
    14881 tgaggtcatt tacccgctca ttagtctgcg catccttgct ggctctggtt agcactggcg
    14941 taaacgccgc agaaaaagtg acgttaaagt tagcacacaa cctggaaaga agtcatgttg
    15001 tacatcagag ttttgaagag ctggcgaaag aggtaaaaca gctgtcaaaa ggtaatatgg
    15061 tgatccgtat ttatcccagc agccagatgg gtaatgcgcg agaaactatg gaattgttgc
    15121 aaaatggcgc actggatatg accaaaggtt ccgccagtga tctggagtct tttgataata
    15181 tttatgctat ctataatttg ccgttcttat taagttgttt ttggtgaagt tggtaaagaa
    15241 attatggact caacaaaaga caaaggcttt tttgctttat cagcttatgt agcgggtact
    15301 cgcagtttct atgcgaaaaa accgattaca aagccagaag atcttaaagg actgaaaatt
    15361 cgtgttcagc caagtccgac caccatcaaa atgattgaac tgatgggtgg ttcacccact
    15421 ccaatttcat ttggcgaagt ttatactgcg atgcagcaag gtgtagtaga tggcgcagaa
    15481 aataacgtgc cttcctggat gcagactcga catattgaaa ttgccaaagt tttttctgaa
    15541 gatgaacacg cctcaattcc tgactttttg gttatctcca ccaaaacatg gaataaatta
    15601 cccccagaac agcaacaaat cctcgaaaca gcggcgaaga aatccgaagc ttatcaacaa
    15661 aaattgtggg aaaaaattga cgctgacaca cgtgctcagg cgaaagctat ggggggtgaa
    15721 attgtaaaag tggataaagc gccattccgt aaagccgtgc aacctctgtt tgacgacttc
    15781 aaaaaagatc caaaacaagc tgctttgtta gagaaattcg acaacgctgc tcaataatat
    15841 tccgggccag cctctggccc tgattttcca ggatgcttac aatgatattt aaccgcctga
    15901 aacttgcggt agaccgcgtc attgccgcgt tttctgtcgc cgtaatgctt gctctggttg
    15961 tatgcgtagt ctggcaagta tttagccgct acgtacttaa ccagccaagt acattaaccg
    16021 atgagctggc acgctttttg atgatctggg taggattatt aggtgctgca tataccgtcg
    16081 gcgcgcagcg acatctctct attgatttat ttgctctcgc cctgaataag cgcaaacaat
    16141 tacttcttag tatagtcatt aacgttctca ttttaggttt tgccggttcg gtcattgtga
    16201 ctggtggcct gaaattaatt gataaaacac tggcaacatc acaggtatct gccgctatgc
    16261 aaataccgat gggatacgta tatatcattc tgccattaag tggagtggta atgatgtttt
    16321 atg
//
LOCUS       DADMFL010000089        16291 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.090, whole genome shotgun sequence.
ACCESSION   DADMFL010000089 DADMFL010000000
VERSION     DADMFL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.090"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(282..986)
                     /locus_tag="JNZ27_002896"
     CDS             complement(282..986)
                     /locus_tag="JNZ27_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY8665154.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRTFGNRR"
     gene            complement(1123..1575)
                     /gene="moaE"
                     /locus_tag="JNZ27_002897"
     CDS             complement(1123..1575)
                     /gene="moaE"
                     /locus_tag="JNZ27_002897"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY8665155.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1577..1822)
                     /gene="moaD"
                     /locus_tag="JNZ27_002898"
     CDS             complement(1577..1822)
                     /gene="moaD"
                     /locus_tag="JNZ27_002898"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY8665156.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1815..2300)
                     /gene="moaC"
                     /locus_tag="JNZ27_002899"
     CDS             complement(1815..2300)
                     /gene="moaC"
                     /locus_tag="JNZ27_002899"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY8665157.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2303..2815)
                     /gene="moaB"
                     /locus_tag="JNZ27_002900"
     CDS             complement(2303..2815)
                     /gene="moaB"
                     /locus_tag="JNZ27_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY8665158.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2837..3826)
                     /gene="moaA"
                     /locus_tag="JNZ27_002901"
     CDS             complement(2837..3826)
                     /gene="moaA"
                     /locus_tag="JNZ27_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY8665159.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3814..3958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4223..5131
                     /gene="yvcK"
                     /locus_tag="JNZ27_002902"
     CDS             4223..5131
                     /gene="yvcK"
                     /locus_tag="JNZ27_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY8665160.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5323..7344)
                     /gene="uvrB"
                     /locus_tag="JNZ27_002903"
     CDS             complement(5323..7344)
                     /gene="uvrB"
                     /locus_tag="JNZ27_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY8665161.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7923..8600)
                     /gene="bioD"
                     /locus_tag="JNZ27_002904"
     CDS             complement(7923..8600)
                     /gene="bioD"
                     /locus_tag="JNZ27_002904"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8665162.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8593..9348)
                     /gene="bioC"
                     /locus_tag="JNZ27_002905"
     CDS             complement(8593..9348)
                     /gene="bioC"
                     /locus_tag="JNZ27_002905"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY8665163.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9335..10489)
                     /gene="bioF"
                     /locus_tag="JNZ27_002906"
     CDS             complement(9335..10489)
                     /gene="bioF"
                     /locus_tag="JNZ27_002906"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY8665164.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDLDRLLEVLHGNG"
     gene            complement(10486..11526)
                     /gene="bioB"
                     /locus_tag="JNZ27_002907"
     CDS             complement(10486..11526)
                     /gene="bioB"
                     /locus_tag="JNZ27_002907"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY8665165.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11613..12902
                     /gene="bioA"
                     /locus_tag="JNZ27_002908"
     CDS             11613..12902
                     /gene="bioA"
                     /locus_tag="JNZ27_002908"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY8665166.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12880..13350
                     /locus_tag="JNZ27_002909"
     CDS             12880..13350
                     /locus_tag="JNZ27_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY8665167.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAETKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13665..13748
                     /locus_tag="JNZ27_002910"
                     /pseudo
     CDS             13665..13748
                     /locus_tag="JNZ27_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            13904..14905
                     /locus_tag="JNZ27_002911"
     CDS             13904..14905
                     /locus_tag="JNZ27_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8665168.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14939..>16291)
                     /locus_tag="JNZ27_002912"
     CDS             complement(14939..>16291)
                     /locus_tag="JNZ27_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY8665169.1"
                     /translation="TDNYQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPG
                     GVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAE
                     DSVIRQVYGWVFDNVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVS
                     SCHFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLD
                     LHMEQPDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKI
                     VSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCI
                     FNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGAT
                     SGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI
                     "
BASE COUNT         3876 a         4291 c         4047 g         4077 t
ORIGIN      
        1 atgagccata taccgtttaa tatcctggcc tcgtgctgaa aatctaattt attgctgttt
       61 tgcgagtatg attccattct tttagttgtc cttagaaaaa atattaatat tcaccatcaa
      121 taaatttaaa accacgcata caaaatactt ttgtatttat ggatacggaa aacgattccg
      181 ctcgctcata aggcatattt tatacctggt acttgttaag tgtcgttttt agtaagaaaa
      241 aaatcccgac acatggccgg gatagtgatg gtgaaaaaga attaacggcg gttgccgaag
      301 gtccgtaaca acatcaggaa caggttgatg aagtccagat acaaggttaa cgcgccaaga
      361 atggaatatt tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc catatttttc
      421 aatttctgag tgtcatacgc cgtcaatccg acaaagacaa tcacgccgat gtaggtaact
      481 gcccacatca atgcttcgct tttcagccag aagttgacca gcgatgccag cacaatgccg
      541 attaacgcca taaacagcat attgcccaag ccacttaaat cgcgcttcgt ggtgtaaccg
      601 tacaggctca ttgcgccgaa catcccggca gtaacgacga aagtactggc gatagaagca
      661 gcggtataga caatgaatat actggaaagc gtaagacccg tcagcgccga ataaagcata
      721 aagagcatcg tcgttacacc tgcgctcagc ttttgaatca tcgctgataa cacaataacc
      781 aatgctaatt gcgcgatgat cagaccgatt aaaaagacac ggttagtgaa caacagctcc
      841 atcacggccg cggaattagc cgcataccag gcaacaaatg cggtcagcaa caagccaacg
      901 gtcatccagc catagacttg cgccatataa gtttgcaagc cagcccgggg ttgtacgatt
      961 gaatcagaac gtgggaatct gtccatgacg atctcctgaa gatataagga atatcttaag
     1021 gatactgcaa aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg
     1081 aaattgccgg acgcagcggt gccttatccg gctaacaaaa aactaccagc gttttgccgc
     1141 ctgcagatcg ctctcccggg cttcaaccca gcggtcgcct tccggcgtgg cttcgcgctt
     1201 ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact
     1261 gctgcgatgc gcactggtga caccgacaaa aacgatttca tcgcccggcc ataattcccc
     1321 gatgcggtga atcaccgtga cgcgccccag cggccagcgg ttacgcgctt catcaacaat
     1381 ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg ctttgacgct
     1441 gtcgcccagg ttatggttgc gcacctttcc ggtgaaggtg actaccgcac cgtcttcgtc
     1501 acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcgggc cgacaacaat
     1561 tttggtttct gccatcttaa cctccggtta ccggcgggag gaaagctact tcgtcgccgt
     1621 cagtcagcgg atggtcaaaa ctcaccagcg tctggttgac ggcagccagc aatttgccat
     1681 cttccagcgc cagcgcccag cgatcgctct gcgcagccag gtgctggcgt aacgcttcaa
     1741 cggttgggta atccgcagcc acttcggttg catctgttcc caccaactcg cgcacctggg
     1801 cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc
     1861 ttcgccagca aacgtaccgg accaatcacc atatcttttt gcaccgcttt gcacatgtca
     1921 taaatggtca gcgccgccac ggaggccgcg gttaacgctt ccatttcgac accggttttc
     1981 ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa
     2041 ttgacttcaa ctttgctgag catcagcggg tgacacagcg ggatcagatc ccaagtgcgt
     2101 tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga
     2161 ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct
     2221 tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg
     2281 atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg
     2341 tacgggcatc cagctgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg
     2401 aacccggcat ggcgaaaatc agcgttttgt tagcgacgcc cgctaccgca cgagattgca
     2461 acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa
     2521 cctcacggtc gaacaacggc agcagcgctt cgggagcctg atcaccttcc gtcaggccag
     2581 taccacccgt aatcaatacc acttgtacat cgtcgctggc gatccacgca gatacctgag
     2641 cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga tggcccgctt
     2701 cttgcgccga atcgcgcaga tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag
     2761 aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat
     2821 ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt
     2881 tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca
     2941 gcgcctgttg ctgggtatcg tcttccagca gatcgcgcag gttaacgccg ccttcaccaa
     3001 acaggcagag atggagttta ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga
     3061 agtctttttc atacggcatg ataaggccaa tctctccggc gtaatctgga tggcaaaaga
     3121 cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct
     3181 cgtcacgcag aacctgacca gagatgtgat gcttacggaa gagctcgctg ccctcgcccg
     3241 tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg
     3301 tgtcgagctg gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa
     3361 aaccggcctc aaatgcagca tcaatccctg ccatgacctg gttgaattta tcctgcccgg
     3421 taatagcgtg aaactggcgg gcgtccaaac tgtcgacact gacgttaatg ccagtaagtc
     3481 ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa
     3541 tctggcggat agcgtcgttt tcccgcacag cggcgatgat atcggtaaag tcgcggcgta
     3601 aagacggctc tcctcctgtc aggcgcactt tttccgtgcc caggctggcg aaggcgcgcg
     3661 taacccggcg aatttcatcg acggtaagaa agcctttatt ggtgacgccg ctcggtttgt
     3721 agccatccgg caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca
     3781 agtagtaaaa cttacgcgca aatgcatcag tcagttgtga agccatgtac acctttccag
     3841 atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca
     3901 cggccaaagc accgtatcag ttgacccagg tgcagaggct agagtgttta gtggttaagc
     3961 cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgca atcatgtata
     4021 tagcgtcatg atcgtgctat ttcgctacag aataagggaa aatggcggtt ttactttgat
     4081 atacatcatc agggaggcat agcgtgcatc gtcctttaga gatagaagaa aatagtttgt
     4141 atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga
     4201 taatctgact ccaggaaact atatgcgcaa tcgtacgttg gctgatcttg atcgtgtcgt
     4261 tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc
     4321 tcgtttaacg ggtatcgtca ccaccacgga taatggtggc tcgacggggc gtattcgccg
     4381 ttcagaaggc ggcattgctt ggggcgatat gcgcaactgc ctcaaccagc tgataacgga
     4441 accgagcgtc gcctccgcga tgtttgaata ccgttttggc ggcaatggcg aactttccgg
     4501 tcataatctc ggaaacttga tgttaaaggc gctggatcac cttagcgtgc ggcctctgga
     4561 agccatcaat ttaattcgta atctgctgaa agtggatacg catttgattc caatgtcaga
     4621 gcatcctgtt gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa
     4681 tatcgaccag ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac
     4741 gcgtgaggcg gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt
     4801 ttataccagc ctgatgccaa ttctgctgct gaaggaaatc gcccaggcat tacgccgcac
     4861 gccagcgccg atggtttata tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa
     4921 tttgaagctg gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga
     4981 tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca
     5041 ggaggtactg gaggccagcg atattccgta tcgtcatgac cgccagttgt tacataacgc
     5101 gctggaaaag gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc
     5161 tattgaagac aaagtgcgcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct
     5221 acaaaccgcg caaattcaat atattgcgga gaaaatgtag gcctgataag cgtagcgcat
     5281 caggctgttt tccgtttgtc atcagtcttc ttcgctatcc tgttacgatg cggcaataaa
     5341 caactcacgc aactgatgca actggtcacg aatttgtgcc gcttcttcga actccagatt
     5401 ctgcgcgtgt tgcatcatca gcccttccag ttcatggatt ttctgctgca acgctttcgg
     5461 cgacatatcc atcggcacat tatccggctc aacaatcggg cgcgattttc ctctgccctt
     5521 cgctttggtt ttggcaatgt tctgccccag cgccaggata tcgaccactt tcttgttcaa
     5581 gccttgcggc gtaatgccgt gttcctcgtt gtacttctgc tgtttctcac ggcgacgttc
     5641 ggtttcacca atcgctttcg ccattgatgg agtgatctta tcgccgtaga gaatcgcttt
     5701 accgttaacg ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt cggaacgcag
     5761 gaagccttct ttgtcagcgt cgaggatcgc caccagcgac acttccggca tatccagacc
     5821 ttcacgcagt aagttgatcc ctaccaatac gtcgaactca cccagacgca agtcgcggat
     5881 aatctccata cgctcgacgg tgtcgatatc tgagtgaaga taacgcacgc gctcaccgtg
     5941 ttcttcgaga tattcggtaa gatcttccgc cattcgcttg gtcagtgtgg tgaccagcac
     6001 gcgttcgtta attgccgctc gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc
     6061 caccggccgc acttcgataa tcgggtcaag caatccggtt ggacgcacca cctgatccac
     6121 cacatcgccg ccggattttt ccagctcgta attacccggc gtcgccgaaa catagatggt
     6181 ttgcggcgct aatgcttcga actcttcaaa cttaagcgga cggttatcca gcgctgatgg
     6241 caggcggaag ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc
     6301 gccaatttgt ggaatggtga cgtgagattc atcgacgacc agcagcccat cggcaggcag
     6361 gtaatcaaac agcgtcggcg gtggctcacc cggtccacga ccggagagga agcgcgagta
     6421 gttttcaatc cccgaacagt agcccagctc gttcatcatc tccagatcaa actgggtacg
     6481 ctgggtcagc cgctgctctt ccagcagttt gttgttttcc aacagcactt tgcgtctggc
     6541 ggccagttct tctttgatct cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta
     6601 gtgcgttttc gggtagatgg taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg
     6661 gtcaaataac gacaatcgtt ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc
     6721 atccgattct gccgggaaga tatctatcac ctcgccacga acgcggaaag taccacgctg
     6781 gaatgcttga tcattacgag cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg
     6841 atcgataatc ataccgaccg tgagatggag catcatcttg agatataaat caggatcgcc
     6901 cagaccataa atcgcggaaa cagacgccac tacaatcaca tcacgccgct ccagcatcgc
     6961 tttggtggcg gacaaacgca tctgctcaat atgttcgtta accgaggcat ctttctcaat
     7021 gaaggtgtcg gaactcggta cataggcttc cggctgatag tagtcgtagt aggagacgaa
     7081 atactccacc gcgttttccg ggaagaactc tttcatttcg ccatacagct gggccgccag
     7141 cgttttgttg ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga cattggcaat
     7201 ggtgaaggtt ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc
     7261 cagcccctct tcgagacgtc gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga
     7321 attcagtttg aacggtttac tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg
     7381 taattttact cgtcgtactt aattatgcca acaaattata ctggataaaa aaacagttca
     7441 tcaccataat atttctgata cagcgtaaac tccgctgtca atcatgagca aaatttactc
     7501 tgtggcgaga taaaactccg gccttaccgg gttatcccca aagcaacggc ttttttaaca
     7561 tttgtcaaga tgagtgatga cagttttatg gcaagagatg cctgtacagt gactgccatt
     7621 gcatttatct aaccagttaa aaaataaaag atatttcttt gagccgtctt taacgccaat
     7681 tcgcgtgcaa gccgcgtatt ctctcgcttg cctcgtgttt tctaactcta atacacatgg
     7741 ttatccacag gaatagtgga taactgtctc cagcccctat cccccgccgc ctgggcaatt
     7801 cccacattcg ccgcatgagg cggaagtaaa atttttttcg tgattatttt agaatttaat
     7861 tggttaaatt gcagtcaatc gaagacgcga tctcgctcgc aatttaacca aatacagaat
     7921 ggctacaaca aggcaaggtt tatgtacttt ccggttgccg cattttctgg attttctgca
     7981 agccagggga tctctcccag cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat
     8041 tcagcgtgac gttttcccgg aggcgtaaca tcgttcgcca cccaacccgc cagagtcagt
     8101 ccggcgtgtt gtattacctg cgcagtcaac atcgcgtgat taatacagcc gagtttcaca
     8161 ccaactacca gtatcaccgg cagttgttcc tgtgttaccc aatctgcaaa agtgaaagtg
     8221 tcagaaagcg gcgtaaacca gccgccagca ccttccacta acacccagtc agcctgttgt
     8281 tcaagcgcgc gtaatccggc gctcattacc aatgattcta tcggcctgcc ctcttgggcg
     8341 ctgatgatgt gcggcgagga gggttctgcg aaggtgtaag gatttaccgt tgcgtaatcc
     8401 agctgcaggc tgctgttgtg ctgtaacgcc agcgcgtcac tattacgtaa gccttccggg
     8461 gtcttttcgc tgccagaggc gaccggttta taacctgccg tccgataacc tactgccttc
     8521 gcggcttgta aaagtgcaca actggcgacg gttttcccca cttcggtatc cgttccggtg
     8581 acaaaataac gtttactcac gagcaatcac tcccaaaaaa agatgatacg tcagaggata
     8641 tcgcccctgt tgttgcggcc aggccagttg caatcgctgc aactgcgaac gcgttaatat
     8701 tcgcgggtcg cgcccttcat gaagatgcgt agcaccgatg cctttcagcg aacgcatggc
     8761 actgagcgca tcatcaaacc atagtgtgat gagctgaata tgatgttgat aacgcacgcc
     8821 gttcagcgac tcttcgattt catctggcgg taaaaagcga ttagcatgcg gacgctcgtc
     8881 caccgcctgc cgcgcctgat gcagttcggg taacgatccc tgcaccagcg tggtaaacgc
     8941 gaccacgcct ccggggcgca ccacccgata cagctcgcgg agtgccgtgg ataaattacc
     9001 gcaccactgc accgcgagat tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag
     9061 ggattcgata tctcccgcca gataatgatc tgcggcatct ttatggcggg cttgcgcaag
     9121 cattggcggc gagagatcta acgccgtcac ctgcgcgtga cgttcccgcc agtggcggct
     9181 catccagcca ggtccacaac ccgcgtccag tacgtgggtg tatttacgct gtggaagcat
     9241 tgccagtaag gcgtcagcac tctggcgctg tagatctgca tgttgctcat agtgtgcggc
     9301 tgcccgacca aatgccgctg caatggcttg tttattaacc gttgccatgc agcacctcca
     9361 gcagacggtc gagatcctgc atttcatgcg cagcggttag cgttaagcgc agtcgcgcag
     9421 taccagcggg tacggttggc gggcgaatcg ccgtgaccca gcagccttgc tggcgcagtt
     9481 tttctgccag ttgtaacgca cggctgttat caccgacgat caatggctgg atggcgctgc
     9541 atgaatcagc cagcgtaaac ggcaaatcct gtactccggc acgaaaacgc gcaatgagtg
     9601 acaccagttt ttcgcgccgt gcatccccct catcacggcg aatgaccgcc agcgacgcac
     9661 gtaatgcctg cgcctgagcg ggcggcatac tggtgctgta gataaggtgg cgggcgaatt
     9721 gcagcagata atccgccacc gtacttgagc aaagcaccgc agccccactg acgccaaatc
     9781 ctttgccaaa agtcactacc agcaattctg gttttacctt ttgcagccag cagctgccgc
     9841 gcccctgctc cccgataacg cccgtgccgt gggcatcatc gaccatcaac cagccattgt
     9901 gctgttgcgt tacctgctgg atttccgcca gtggcgcgct atcgccgtcc atgctgaaca
     9961 cgccttctgt caccaccagt tgctgccccg gacaggggga agcaagcagt cgcgccagat
    10021 gagcgacatc gttatgagta aaacggcgaa gctgcgacgg gcttaaactg gcagcctcca
    10081 gcaatgaggc atggctaagc cggtcggcaa caatacggtc ctctttcgcc atcatcgcgg
    10141 caatgactgc ctgattcgcg gcaaaaccag agataaacag cagcgcccgc gaatagccaa
    10201 gccactcggc cagttcttct tccagcgcct gatgcaccac gctataaccg ctgacgtgac
    10261 cggagccgcc gctaccgatg ccaaattgct ccgccccctg cttccaggca cggataattt
    10321 gcggatgatg gcttaaacct aaataatcgt tactggaaaa gttcagatac tggcgatcat
    10381 ccgccaccag ccagcgtccg gctccttgcg ccaccggata acggcgacgc agggcatcgg
    10441 cagcacgccg cgcatcgagc gccgcgttga ttttctcctg ccagctcata atgctgccgc
    10501 gttgtaatat tcgtcggtgt ccggggtcat cagcgcctgt ccaagacgtt gctgttgttc
    10561 gttatcccct gccagcacgg cagtttgctg cggatttagc cccagtttgc ggaacagttg
    10621 caggtcttta tcttcttccg gattcggcgt ggtcagcagt ttgcaaccgt agaaaatcga
    10681 gtttgcgcct gccataaagc acatcgcctg agtctgttcg ttcatctgct cgcgtccggc
    10741 agaaaggcgc acgtaagagg tcggcatcat gatccgcgct acggcaatgg tgcgaataaa
    10801 atcaaaggca tcaacgtcat cgttatcggc aagcggcgtg cctttcacct tcaccagcat
    10861 gttgattggt acgctttccg gcggcgtcgg caggtttgcc agttgcagca ataatccggc
    10921 gcgatctttt accgtttcgc ctaagcccac aatgccacct gagcagactt tgatcccggc
    10981 atcgcgcact ttttccagcg tatcgaggcg ttcctgataa gtgcgtgtgg tgatgatatt
    11041 gccgtaaaac tccggcgagg tgtccaggtt gtggttgtag taatccagcc cggcgttcgc
    11101 gaggcgctgc gcctgagatt cactcaacgt gcccagcgtc atacacgcct ccagccccat
    11161 cgcttttacc ccctgcacca tttgttccag gtacggcata tcgcgttcgt ggggattctt
    11221 ccatgccgcg cccatacaga agcgcgtcga tcctgccgct ttcgctttgc gcgccgactc
    11281 cagcacctgt tcaacttcca tcaaccgctc ggcttccagc ccggttttgt agcgcgagct
    11341 ttgcgggcag tatttgcaat cttccggaca agctccggtc ttaatcgaca gcaacgtgct
    11401 gacctgcacc tgacgaggat cgaaatgctg gcgatgcacc tgctgcgctt caaacagcag
    11461 atccagcaac ggtttttcaa ataattctgt gacttgcgac aatgtccagc gtgggcggtg
    11521 agccatgggg cttctccaaa acgtgttttt tgttgttaat tcggtgtagg cttgtaaacc
    11581 taaatctttt caatttggtt tacaagtcga ttatgacaac ggacgatctt gcctttgacc
    11641 aacgccatat ctggcaccca tacacatcca tgacctcccc tctgccggtt tatccggtgg
    11701 cgagcgccga aggttgcgag ctaattttgt ctgacggcag acgcctggtt gacggtatgt
    11761 cgtcctggtg ggcggcgatc cacggctaca atcacccgca gcttaatgcg gcgatgaaga
    11821 cgcaaattga tgccatgtcg catgtgatgt ttggcggtat cacccatgcg cccgccatcg
    11881 agctatgccg caaactggtg gcaatgacgc cgcaaccgct ggagtgcgtt tttctcgcgg
    11941 actccggttc cgtagcggtg gaagtggcga tgaaaatggc gttgcagtac tggcaagcca
    12001 aaggcgaagc gcgccagcgt tttctgacct tccgcaatgg ttatcatggc gatacctttg
    12061 gcgcgatgtc ggtgtgcgat ccggacaact caatgcacag cctgtggaaa ggctatctgc
    12121 cagaaaactt gtttgctccc gccccgcaaa gccgcatgga tggtgaatgg gatgaacgcg
    12181 atatggttgg ttttgcccgc ctgatggcgg cgcatcgtca tgaaatcgca gcggtgatca
    12241 ttgagccgat tgtccagggc gcaggcggga tgcgcatgta ccatccggaa tggttaaaac
    12301 gaatccgcaa aatgtgcgat cgcgaaggta tcttgctgat tgccgacgag atcgccaccg
    12361 gatttggtcg taccggcaaa ctgtttgcct gtgaatatgc agaaatcgcg ccggacattt
    12421 tgtgcctcgg taaagcctta accggcggca caatgaccct ttccgccaca cttaccacgc
    12481 gcgaggttgc agaaaccatc agtaacggcg aagccggctg ctttatgcat gggccaactt
    12541 ttatgggcaa tccgctggcc tgcgcggcag caaacgccag cctggcgatt atcgaatccg
    12601 gcgaatggca gcagcaggtg gcggctattg aagtgcagct gcgcgagcaa ctggcaccag
    12661 cccgtgatgc cgaaatggtt gccgatgtgc gcgtactggg ggcaatcggt gtggtcgaaa
    12721 ccactcgtcc ggtgaatatg gcggcgctgc aaaaattctt tgtcgaacag ggggtatgga
    12781 tccggccttt tggcaaactg atttacctga tgccgcccta tattattctc ccgcaacagt
    12841 tgcagcgtct gaccgcagcg gttaaccgcg cggtacagga tgaaacattt ttttgccaat
    12901 aatggcgata agttgccgca tcgtcatgtc tttaacggca tgggttacga tggcgataat
    12961 atttcaccgc atctggcgtg ggatgatgtt cctgcggaaa cgaaaagttt tgttgtcacc
    13021 tgctacgacc cggatgcgcc aaccggctcc ggctggtggc actgggtagt tgttaattta
    13081 cccgctgata cccgcgtatt accgcaaggg tttggctctg gtctggtagc aatgccagac
    13141 ggcgttttgc agacgcgtac cgattttggt aaaaccggat acgatggcgc agcgccgccg
    13201 aaaggcgaaa cccatcgcta catttttacc gttcacgcgc tggatgtaga gcgtattgat
    13261 gtcgatgaag gaggcagcgg cgcgatggtc gggtttaacg ttcatttcca ctctctggca
    13321 agcgcatcga ttaccgcgat gtttagttaa tcactctgcc agatggcgca atgccatctg
    13381 gtatcactta aaggtattaa aaacaacttt ttgtcttttt accttcccgt ttcgctcaag
    13441 ttagtataaa aaagctgaac gagaaacata aaacgctgta aatatcaata tgttatgaaa
    13501 gatttagtct aaataataga ctgcataata ctacaaaaca caacatatcc agtcactatg
    13561 aatcaactac ttagatagta ttagtgacct gaggcagagc attagcgcaa ggtgattttt
    13621 tgtcctcttg cgctaatttt ttgtcaacgg actgggttag ttgaatttac aaccatgcta
    13681 agtatctcga taagcgcaga gaaatgatgc agtggtgggc ggattggctt gatgagaagg
    13741 ttaggtaggc aaatccatgc ctgagcagag ccttgccctc tacctgagcc catgctcatt
    13801 ctttggcctc aacgcgagca tgacctcact ggctggaccc aaaatggact atgacagaaa
    13861 gatctgatat ttttcctcgc aaaaacttag aggctttatt ttaatgcata agagcaactg
    13921 ctttgacacg gcaaggcttg tggcagctat gatggttctg gtcagtcacc actacgcgct
    13981 ctctgggcag ccggagccgt acctgttcgg ctttgaaagt gctggcggca ttgcagttat
    14041 catattcttt tcaatatcag gatatctgat ttcaaaatca gctatcagga gtgattcctt
    14101 tattgatttc atggctaaaa gagcgagaag aatattcccg gcgctggtcc cttgctctat
    14161 actgacatat tttttatttg gatggatact taacgacttc agcgcagaat attttagcca
    14221 tgacattgtc aggaagacca taagctctat ttttatgagt caggctccag atgctgacat
    14281 tacatcacat ttaattcatg ccggaattaa cggcagtttg tggaccctac cgcttgagtt
    14341 cctgtgttat ataataacgg gagtagcagt tgccctacta aagaacggaa aagcattcat
    14401 agttatactg ttggtttttg tttctctttc attaattgga agtgtgtcag aaaacaggga
    14461 tgtaatgttc tctattccgt tatggctgta tcctttgcgt ggactggcgt tcttttttgg
    14521 tgccactatg gcaatgtatg agaagtcatg gaatgtaagt aatgttaaaa ttactgtagt
    14581 ttctttgctt gctatgtacg cctacgctag ttatggcaaa ggcattgatt atactatgac
    14641 ttgctatatc ctggtttcat tctcgacaat agccatatgc acatccgttg gcgacccact
    14701 ggttaagggc aggtttgatt actcgtatgg tgtgtacatt tatgcattcc cggttcagca
    14761 ggttgttata aacacattgc acatgggttt ttacccgtca atgttgttaa gtgccgtgac
    14821 tgttcttttc ttgtcccatc tttcatggaa tctggttgaa aaaagattcc tgacacgaag
    14881 ttcgcctaaa ttatccctgg attgaacaat aaaaggggcc atatggcccc taaaataatc
    14941 agatggccag atactcaacc attgaaggca gcgcgactcc tactgggctt gggcgagtaa
    15001 cattaattgt caccacggaa acactaacct tgtcaccact tgatgcaggg tcaccaaaaa
    15061 ggcttcttac tctgtatgtc aggttccccg ggttcggtat agctgcagat ccgcttgtgg
    15121 cacctaccgc aactgggaca ttaatgatcc catttacaga cggtacgcct gaacagccac
    15181 gatacgtatt gtatctgcct tccggtttct gcacctcggt aatgccagcc aaataacagt
    15241 cagtaactga cacgccaccg ctacgaagat agcatagagg cttattgaat atgcacttat
    15301 cgaaagaaac acattcattg atgtcgacaa tatttacagc ccattcatca ccagttaacg
    15361 tacaaccact tacaaagacg aagatcgccg actgttctat cgctatactt tgattgtttg
    15421 cagccgtgtt tttgtttgca gtgttgatat gaacaccact gacaatttta tgtgactgcg
    15481 ttgaggtcgg cgttctaaaa tatatccccg taaattgttc agtgccatcg gcatctgctg
    15541 ctatccatga gtttgagaaa gaaaatcctc cgtttacatt ctctataacc acgcctgttg
    15601 aaccgcaata atctaaatcc ggttgttcca tatgcaaatc aaggcaatca tgaacataga
    15661 cggcattttt aaaaccaatg cacatggtct cactgtctaa aattattgct tctgacctaa
    15721 ctgcctctga cgaccacgca tacgtttgag gctgaatcct gatgccaaag ctttcatcag
    15781 cagaataatt tcctctgctg aaatggcagg agctgacact aacacttacg cactggccta
    15841 aaaagtgaag accgactcta caggttccag cctgacatgc tataaatttt gagtgccaga
    15901 gcccttgcat taaataagcg gtttcaactt cattcaccat tacattatca aatacccagc
    15961 cataaacctg tctgatgaca gagtcttctg ctgtaatgtt tgtgtcaata ccattgcagg
    16021 tgttattacc agttactcgt atatttctca gataacggcc gatagtgtct gaatcataag
    16081 acagccttaa cacagatcct gtaagtccgc tttttgctcg gagttgcgat gccttaccaa
    16141 cccctctgat gttaacaccg ccaggaataa gcaaagtgga tccaacaata taccccaccg
    16201 cttggttgct cgcagggata aacagctccc cgccggaggg tagtgaagca acggcatcta
    16261 ttgcattttg tattgcctgg taattatctg t
//
LOCUS       DADMFL010000090        15961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.091, whole genome shotgun sequence.
ACCESSION   DADMFL010000090 DADMFL010000000
VERSION     DADMFL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.091"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNZ27_002913"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNZ27_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY8665170.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLSGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JNZ27_002914"
     CDS             complement(998..2020)
                     /locus_tag="JNZ27_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8665171.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JNZ27_002915"
     CDS             complement(2034..3536)
                     /locus_tag="JNZ27_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8665172.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNZ27_002916"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNZ27_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JNZ27_002917"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JNZ27_002917"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY8665173.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JNZ27_002918"
     CDS             complement(5853..6194)
                     /locus_tag="JNZ27_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8665174.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNZ27_002919"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNZ27_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY8665175.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNZ27_002920"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNZ27_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY8665176.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JNZ27_002921"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JNZ27_002921"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY8665177.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JNZ27_002922"
     CDS             12873..14216
                     /locus_tag="JNZ27_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8665178.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JNZ27_002923"
     CDS             complement(14278..14484)
                     /locus_tag="JNZ27_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY8665179.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15372
                     /locus_tag="JNZ27_002924"
     CDS             14809..15372
                     /locus_tag="JNZ27_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8665180.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGQDG
                     RVQWAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15435..>15961)
                     /locus_tag="JNZ27_002925"
     CDS             complement(15435..>15961)
                     /locus_tag="JNZ27_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665181.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3799 a         4373 c         3925 g         3864 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgaaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccctaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga cactgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg ctcctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agccaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 ccctggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gaagaaagtt ccgctgtagt ggtgctggat aaagacggtc aagacggtcg cgtgcaatgg
    15301 gccaaagacg gggcgctcac accggaagag gtgcagcaag tgatggacct gctgcaaaag
    15361 ttgctcaaat aatggcaata acataaattc tggaaaaata attttcccct atcgttatct
    15421 atgggatagg ggaattaagt ctctgagttc ttacaccgaa ctaatactgg taacgctagt
    15481 tgctcttttt taaacgcctc aatgatcgat ttatatttct cgcaaatctt ttctttggta
    15541 atattactat ctgcggccag attcgcactt actatcgcat ccacttgttc ggctaattcg
    15601 acttgttggc aacctgcatt aaccagaaaa ttataaataa gagagtaatc ttcatcctcc
    15661 aaaacaacag aggaaccatt acgaaaacgt tcatcccctc catagatttt aagcgcaaag
    15721 cccaaagatt ctatgactat ggcaaaatta tcatcaatta ttttttcgta aatataccat
    15781 ccatcgcatt ctttaattcc accgcctggt atttggagtt caaatttacc ccgttttaat
    15841 ttgcttacta catcctccaa taaggcataa ccgaaagata cgtcaccaaa cgcctggcga
    15901 atataacctt taatcctttt tggtatgtca gactgttttt tctgcaaaat atcacaggca
    15961 t
//
LOCUS       DADMFL010000091        15567 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.092, whole genome shotgun sequence.
ACCESSION   DADMFL010000091 DADMFL010000000
VERSION     DADMFL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.092"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            242..436
                     /gene="bfd"
                     /locus_tag="JNZ27_002926"
     CDS             242..436
                     /gene="bfd"
                     /locus_tag="JNZ27_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY8665182.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            509..985
                     /gene="bfr"
                     /locus_tag="JNZ27_002927"
     CDS             509..985
                     /gene="bfr"
                     /locus_tag="JNZ27_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY8665183.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(982..1449)
                     /locus_tag="JNZ27_002928"
     CDS             complement(982..1449)
                     /locus_tag="JNZ27_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY8665184.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1828..2139
                     /gene="rpsJ"
                     /locus_tag="JNZ27_002929"
     CDS             1828..2139
                     /gene="rpsJ"
                     /locus_tag="JNZ27_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY8665185.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2172..2801
                     /gene="rplC"
                     /locus_tag="JNZ27_002930"
     CDS             2172..2801
                     /gene="rplC"
                     /locus_tag="JNZ27_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY8665186.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2812..3417
                     /gene="rplD"
                     /locus_tag="JNZ27_002931"
     CDS             2812..3417
                     /gene="rplD"
                     /locus_tag="JNZ27_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY8665187.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3414..3716
                     /gene="rplW"
                     /locus_tag="JNZ27_002932"
     CDS             3414..3716
                     /gene="rplW"
                     /locus_tag="JNZ27_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY8665188.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3734..4555
                     /gene="rplB"
                     /locus_tag="JNZ27_002933"
     CDS             3734..4555
                     /gene="rplB"
                     /locus_tag="JNZ27_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY8665189.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4572..4850
                     /gene="rpsS"
                     /locus_tag="JNZ27_002934"
     CDS             4572..4850
                     /gene="rpsS"
                     /locus_tag="JNZ27_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY8665190.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4865..5197
                     /gene="rplV"
                     /locus_tag="JNZ27_002935"
     CDS             4865..5197
                     /gene="rplV"
                     /locus_tag="JNZ27_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY8665191.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5215..5916
                     /gene="rpsC"
                     /locus_tag="JNZ27_002936"
     CDS             5215..5916
                     /gene="rpsC"
                     /locus_tag="JNZ27_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY8665192.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5929..6339
                     /gene="rplP"
                     /locus_tag="JNZ27_002937"
     CDS             5929..6339
                     /gene="rplP"
                     /locus_tag="JNZ27_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY8665193.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6339..6530
                     /gene="rpmC"
                     /locus_tag="JNZ27_002938"
     CDS             6339..6530
                     /gene="rpmC"
                     /locus_tag="JNZ27_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY8665194.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6530..6784
                     /gene="rpsQ"
                     /locus_tag="JNZ27_002939"
     CDS             6530..6784
                     /gene="rpsQ"
                     /locus_tag="JNZ27_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY8665195.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6949..7320
                     /gene="rplN"
                     /locus_tag="JNZ27_002940"
     CDS             6949..7320
                     /gene="rplN"
                     /locus_tag="JNZ27_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY8665196.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7331..7645
                     /gene="rplX"
                     /locus_tag="JNZ27_002941"
     CDS             7331..7645
                     /gene="rplX"
                     /locus_tag="JNZ27_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY8665197.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7660..8199
                     /gene="rplE"
                     /locus_tag="JNZ27_002942"
     CDS             7660..8199
                     /gene="rplE"
                     /locus_tag="JNZ27_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY8665198.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8214..8519
                     /gene="rpsN"
                     /locus_tag="JNZ27_002943"
     CDS             8214..8519
                     /gene="rpsN"
                     /locus_tag="JNZ27_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY8665199.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8553..8945
                     /gene="rpsH"
                     /locus_tag="JNZ27_002944"
     CDS             8553..8945
                     /gene="rpsH"
                     /locus_tag="JNZ27_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY8665200.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8958..9491
                     /gene="rplF"
                     /locus_tag="JNZ27_002945"
     CDS             8958..9491
                     /gene="rplF"
                     /locus_tag="JNZ27_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY8665201.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9501..9854
                     /gene="rplR"
                     /locus_tag="JNZ27_002946"
     CDS             9501..9854
                     /gene="rplR"
                     /locus_tag="JNZ27_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY8665202.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9869..10372
                     /gene="rpsE"
                     /locus_tag="JNZ27_002947"
     CDS             9869..10372
                     /gene="rpsE"
                     /locus_tag="JNZ27_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY8665203.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10376..10555
                     /gene="rpmD"
                     /locus_tag="JNZ27_002948"
     CDS             10376..10555
                     /gene="rpmD"
                     /locus_tag="JNZ27_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY8665204.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10559..10993
                     /gene="rplO"
                     /locus_tag="JNZ27_002949"
     CDS             10559..10993
                     /gene="rplO"
                     /locus_tag="JNZ27_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY8665205.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11001..12332
                     /gene="secY"
                     /locus_tag="JNZ27_002950"
     CDS             11001..12332
                     /gene="secY"
                     /locus_tag="JNZ27_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY8665206.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12364..12480
                     /gene="rpmJ"
                     /locus_tag="JNZ27_002951"
     CDS             12364..12480
                     /gene="rpmJ"
                     /locus_tag="JNZ27_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY8665207.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12627..12983
                     /gene="rpsM"
                     /locus_tag="JNZ27_002952"
     CDS             12627..12983
                     /gene="rpsM"
                     /locus_tag="JNZ27_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY8665208.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13000..13389
                     /gene="rpsK"
                     /locus_tag="JNZ27_002953"
     CDS             13000..13389
                     /gene="rpsK"
                     /locus_tag="JNZ27_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY8665209.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13423..14043
                     /gene="rpsD"
                     /locus_tag="JNZ27_002954"
     CDS             13423..14043
                     /gene="rpsD"
                     /locus_tag="JNZ27_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY8665210.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14069..15058
                     /gene="rpoA"
                     /locus_tag="JNZ27_002955"
     CDS             14069..15058
                     /gene="rpoA"
                     /locus_tag="JNZ27_002955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY8665211.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15099..15482
                     /gene="rplQ"
                     /locus_tag="JNZ27_002956"
     CDS             15099..15482
                     /gene="rplQ"
                     /locus_tag="JNZ27_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY8665212.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4008 a         3658 c         4148 g         3753 t
ORIGIN      
        1 caatccgtga aggcggccgt accgttggcg cgggcgttgt tgctaaagtt ctgggctaat
       61 tacacgttaa ttagttttga attgaaaagg gcgcttcggc gccctttttg ctgcccgtaa
      121 tttgagctcg aaatatttag tcgtaatttt gctcgctgaa ctatggcgca aataaaactg
      181 tgctattgta atcataacca ttctcattta cactttgtgc agaatttgaa cgggagcaat
      241 catgtacgtt tgtttgtgta atggtgtaag tgacaaaaaa attcgccagg cagtccgtca
      301 gtttcatccg caatctttcc aacaattgcg taagtttatt cctgtgggaa atcaatgcgg
      361 taagtgcatt cgcgccgcgc gcgaagtgat gcaggacgag ttgatgcaga tgccagaatt
      421 taaagagatt gcctgaagcc cacgcttatt ttttgacatc ccgctagccc catctacgct
      481 ctaatagtgg aagcggaggg actataaaat gaaaggtgat gttaaaatca taaattatct
      541 caataaacta ttgggaaatg agcttgtcgc aatcaatcag tattttctcc atgcccggat
      601 gtttaaaaac tggggcctga tgcgtctcaa tgacgtcgaa taccatgaat ccatcgatga
      661 gatgaaacac gctgataaat atatcgagcg tattttgttt ttagaaggaa tccccaactt
      721 acaggattta ggcaaactgg ggattggtga agatgtcgag gagatgctgc aatctgacct
      781 gcgattagaa ctggaaggcg cgaaggatct gcgtgaggct atcgcatatg ccgacagcgt
      841 tcatgactat gttagccgcg atatgatgat cgagatcctt gctgatgaag aaggtcatat
      901 cgactggctc gaaactgaac tggatctcat cggtaaaatc ggcctacaaa attacctgca
      961 atcacaaatt aaagttaaag attaacggcc tatcagcaaa ctatcccagc ctataacgaa
     1021 acccgcagcc gccagaaatg gcccaaaagg cagcgggttt tttaatgatt cttttcctct
     1081 catgagcaga ccaataacta cggctccgca agcgaatgag gcagcaagga aaaccagtcg
     1141 tggtaagaag gcccatgaat gccaggcgcc aagggcggcg agaaacttca catcaccata
     1201 gcctaatcct tctttatgtc gcagtatgcg atacccccag taaatgacag cgaatgtgcc
     1261 gtaaccaaca attgcccccc ataacgcatc ggctaaacca tcaggatgac aaacttgata
     1321 gaacaaaaga cctgaccaga gtaacgggca cgtaaatctg tcaggtaaca agccatgctt
     1381 tgcatcccag aaaaaaagta gggctgataa acaagcgtac aaaattagaa agggaagggt
     1441 agtgtccata aaacatcctc tgtaattgga gtatcaccat cgaataactc cggatgctca
     1501 gcaacagaga gagcgctgat ttgttaacct gattccggtg aaaaacgcta tcttcacaac
     1561 cttacaaatt tcgtggaatg cagctcgcaa tctgctcgtt gcgattaatt tctacccgat
     1621 gcttgcgtca ggctcagatt tgcgtataat gcgcgggctt gtcgtagttg acagcaggtt
     1681 caatctgaac ctcagttagc cgaatttggc tacctaacaa tgctcccaat cggggagcta
     1741 cgtaagaacg gttacactct cccatcaatc gtaatgggtc tgaggagtaa tcattttcgt
     1801 ttataaaata attggagctc tggtctcatg cagaaccaaa gaatccgtat ccgcctgaaa
     1861 gcgtttgatc atcgtctgat cgatcaagca accgcggaaa tcgtcgagac tgccaagcgc
     1921 actggtgcgc aggtccgtgg tccgatcccg ctgccgacac gcaaagagcg cttcactgtt
     1981 ctgatctccc cgcacgtcaa caaagacgcg cgcgatcagt acgaaatccg tactcacttg
     2041 cgtctggttg acatcgttga gccaaccgag aaaaccgttg atgctctgat gcgtctggat
     2101 ctggctgccg gtgtagacgt gcagatcagc ctgggttaat caggtcattg agcgattgag
     2161 aggttgaaac aatgattggt ttagtcggta aaaaagtggg tatgacccgt atcttcacag
     2221 aagacggcgt ttctatccca gtaaccgtaa tcgaagttga agcaaaccgc gttactcagg
     2281 ttaaagacct ggctaacgat ggctaccgtg ctattcaggt gaccaccggt gctaaaaaag
     2341 ctaaccgtgt gaccaagcct gaagctggcc acttcgctaa agctggcgta gaagctggcc
     2401 gtggtctgtg ggaattccgc ctggctgaag gcgaagagtt cactgtaggt cagagcatta
     2461 gcgttgaact gtttgctgac gttaaaaaag ttgacgtaac tggcacctct aaaggtaaag
     2521 gtttcgcagg taccgttaag cgctggaact tccgtaccca ggacgctact cacggtaact
     2581 ccttgtctca ccgcgttccg ggttctatcg gtcagaacca gactccgggc aaagtgttca
     2641 aaggcaagaa aatggcaggt cagatgggta acgaacgtgt aaccgttcag agccttgacg
     2701 tagtacgcgt tgacgctgag cgcaacctgc tgctggttaa aggtgctgtc ccgggtgcaa
     2761 ccggtagcga cctgatcgtt aaaccagctg tgaaggcgta aggagatagc aatggaatta
     2821 gtattgaaag acgcgcagag cgcgctgact gtttccgaaa ctaccttcgg tcgtgatttc
     2881 aacgaagcgc tggttcacca ggttgttgtt gcttatgcag ctggtgctcg tcagggtact
     2941 cgtgctcaga agactcgtgc tgaagtaact ggttccggta aaaaaccgtg gcgccagaaa
     3001 ggcaccggcc gtgcgcgttc tggttctatc aagagcccga tctggcgttc tggtggggtg
     3061 acctttgctg ctcgtccgca ggaccacagt caaaaagtta acaagaagat gtaccgcggc
     3121 gcgctgaaaa gcatcctgtc cgaactggta cgtcaggatc gtctgatcgt tgtcgagaag
     3181 ttctctgtag aagcgccgaa aactaagctg ctggcacaga aactgaaaga catggctctg
     3241 gaagatgtgc tgatcatcac cggtgagctg gacgaaaacc tgttcctggc tgcgcgcaac
     3301 ctgcacaagg ttgacgtacg cgatgcaact ggtatcgacc cggttagcct gatcgccttc
     3361 gacaaagtcg taatgactgc tgatgctgtt aagcaagttg aggagatgct ggcatgattc
     3421 gtgaagaacg tctgctgaag gtgctgcgtg caccgcacgt ttctgaaaaa gcgtctactg
     3481 cgatggaaaa atccaacacc atcgtactca aagttgctaa agacgcgacc aaagcagaaa
     3541 tcaaagctgc tgtgcagaaa ctgtttgaag tcgaagtcga agtcgttaac accctggtag
     3601 ttaaagggaa agttaaacgt cacggacagc gtatcggtcg tcgtagcgac tggaaaaaag
     3661 cttacgtcac cctgaaagaa ggccagaatc tggacttcgt tggcggcgct gagtaagtcg
     3721 gaggagtaat acaatggcag ttgttaaatg taaaccgaca tctccgggtc gtcgccacgt
     3781 agttaaagtg gttaaccctg agctgcacaa gggcaaacct tttgctccgt tgctggaaaa
     3841 aaacagcaaa tccggtggtc gtaacaacaa tggccgtatc accactcgtc atatcggtgg
     3901 tggccacaag caggcttacc gtattgttga cttcaaacgc aacaaagacg gtatcccggc
     3961 agttgttgaa cgtcttgagt acgatccgaa ccgttccgcg aacatcgcgc tggttctgta
     4021 caaagacggt gaacgccgtt acatcctggc ccctaaaggc ctgaaagctg gcgaccagat
     4081 tcagtctggc gttgatgctg caatcaaacc aggtaacacc ctgccgatgc gcaacatccc
     4141 ggttggttct actgttcata acgtagaaat gaaaccaggt aaaggcggtc agctggcacg
     4201 ttccgctggt acttacgttc agatcgttgc tcgtgatggt gcttatgtca ccctgcgtct
     4261 gcgttctggt gaaatgcgta aagtagaagc agactgccgt gcaactctgg gcgaagttgg
     4321 caatgctgag catatgctgc gcgttctggg taaagcaggt gctgcacgct ggcgtggtgt
     4381 tcgtccgacc gttcgcggta ctgcgatgaa cccggtagac cacccacatg gtggtggtga
     4441 aggtcgtaac tttggtaagc acccggtaac tccgtggggc gttcagacca aaggtaagaa
     4501 gacccgcagc aacaagcgta ctgataaatt catcgtacgt cgccgtagca aataatttta
     4561 gaggataagc catgccacgt tctctcaaga aaggtccttt tattgacctg cacttgctga
     4621 agaaggtaga gaaagcggtg gaaagcggag acaagaagcc cctgcgcact tggtcccgtc
     4681 gttcaacgat ctttcctaac atgatcggtt tgaccatcgc tgtccataat ggtcgtcagc
     4741 acgttccggt atttgtaacc gacgaaatgg tcggtcacaa actgggtgaa ttcgcaccga
     4801 ctcgtactta tcgcggccac gctgctgata aaaaagcgaa gaagaaataa ggtaggagga
     4861 agagatggaa actattgcta aacatcgcca tgctcgttct tctgctcaga aggttcgcct
     4921 tgttgctgac ctgattcgcg gtaagaaagt gtcgcaggct ctggatattt tgacctacac
     4981 caacaagaaa gcggctgtac aggtcaagaa agttctggaa tctgccattg ctaacgctga
     5041 acacaacgat ggcgctgaca ttgacgatct gaaagttacg aaaattttcg tagacgaagg
     5101 cccgagcatg aagcgcatta tgccgcgtgc aaaaggtcgt gcagatcgta tcctgaagcg
     5161 caccagccac atcactgtgg ttgtgtccga tcgctgagac tctggagact agcaatgggt
     5221 cagaaagtac atcctaatgg tattcgcctg ggtattgtaa aaccatggaa ctctacctgg
     5281 tttgcgaaca ccaaagaatt cgctgacaac ctggacagcg attttaaagt acgtcagtac
     5341 ctgactaagg aactggctaa agcgtccgta tctcgtatcg ttatcgagcg tccggctaag
     5401 agcatccgtg taaccattca cactgctcgc ccgggtatcg ttatcggtaa gaaaggtgaa
     5461 gacgtagaaa aactgcgtaa ggtcgtagcg gacatcgctg gcgttcctgc acagatcaac
     5521 atcgccgaag ttcgtaagcc tgaactggac gcaaaactgg ttgctgacag catcacttct
     5581 cagctggaac gtcgcgttat gttccgtcgt gctatgaagc gtgctgtaca gaacgcaatg
     5641 cgtctgggcg ctaaaggtat taaagttgaa gttagcggcc gtctgggcgg cgcggaaatc
     5701 gcacgtaccg aatggtaccg cgaaggtcgc gtaccgctgc acactctgcg tgctgacatc
     5761 gactacaaca cctctgaagc gcacaccact tacggtgtaa tcggcgttaa agtgtggatc
     5821 ttcaaaggcg agatcctggg tggtatggct gctgttgaac aaccggaaaa accggctgct
     5881 cagcctaaaa agcagcagcg taaaggccgt aaataaggag cgtcgctgat gttacaacca
     5941 aagcgtacaa aattccgtaa aatgcacaaa ggccgtaacc gcggtctagc gcagggtacg
     6001 gatgttagct tcggcagctt cggtctgaaa gctgttggcc gtggtcgtct gactgcccgt
     6061 cagatcgaag cagcacgtcg tgctatgacc cgtgcagtta agcgtcaagg taagatctgg
     6121 atccgtgtgt tcccggacaa accgatcact gaaaagccgc tggcagtgcg tatgggtaaa
     6181 ggtaaaggta acgtggagta ttgggttgcc ttgattcagc cgggtaaagt cctgtatgaa
     6241 atggacggtg ttccggaaga gctggcccgt gaagcattca agctggcagc agcgaaactg
     6301 ccgattaaaa ccacctttgt aactaagacg gtgatgtaat gaaagcaaaa gagctgcgtg
     6361 agaagagcat tgaagagctg aacaccgagc tgctgaacct gctgcgtgag cagttcaacc
     6421 tgcgtatgca ggctgcaagt ggccagctgc aacagtctca cctgttgaag caagtgcgtc
     6481 gcgatgtcgc acgcgttaag actttactga acgagaaggc gggtgcgtaa tgaccgataa
     6541 aatccgtact ctgcaaggtc gcgttgttag cgacaaaatg gagaaatcca ttgttgttgc
     6601 tatcgaacgt tttgtgaaac acccgatcta cggtaaattc atcaagcgta cgaccaaact
     6661 gcacgtacat gacgagaaca acgaatgcgg tatcggtgac gtggttgaaa tccgcgaatg
     6721 ccgtccgctg tccaagacta aatcctggac gctggttcgc gttgtagaga aagcggttct
     6781 gtaatacagt acactctctc gatacgaata aacggctcag aaatgagccg tttatttttt
     6841 ctacccatat ccttgaagcg gtgttataat gccgcgccct cgatatgggg atttttaacg
     6901 acctgatttt cgggtctcag tagtagttga cattagcgga gcactaaaat gatccaagaa
     6961 cagactatgc tgaacgtcgc cgacaactcc ggtgcacgtc gcgtaatgtg tatcaaggtt
     7021 ctgggtggct cgcaccgtcg ctacgcaggc gtaggcgaca tcatcaagat caccatcaaa
     7081 gaggcaattc cgcgtggtaa ggtcaaaaaa ggtgatgtgc tgaaggcggt agtggtgcgc
     7141 accaagaagg gtgttcgtcg cccggacggt tctgtcattc gcttcgatgg taatgcttgt
     7201 gttcttctga acaacaacag cgagcagcct atcggtacgc gtatttttgg gccggtaact
     7261 cgtgagcttc gtagtgagaa gttcatgaaa attatctctc tggcaccaga agtactctaa
     7321 ggagcgaatc atggcagcga aaatccgtcg tgatgacgaa gttatcgtgt taaccggtaa
     7381 agataaaggt aaacgcggta aagttaagaa tgtcctgtct tccggcaagg tcattgttga
     7441 aggtatcaac ctggttaaga aacatcagaa gccggttccg gccctgaacc aaccgggtgg
     7501 catcgttgaa aaagaagccg ctattcaggt ttccaacgta gcaatcttca atgcggcaac
     7561 cggcaaggct gaccgtgtag gctttagatt cgaagacggc aaaaaagtcc gtttcttcaa
     7621 gtctaacagc gaaactatca agtaatttgg agtagtacga tggcgaaact gcatgattac
     7681 tacaaagacg aagtagttaa aaaactcatg actgagttta actacaattc tgtcatgcaa
     7741 gtccctcggg tcgagaagat caccctgaac atgggtgttg gtgaagcgat cgctgacaaa
     7801 aaactgctgg ataacgcagc agcagacctg gcagcaatct ccggtcaaaa accgctgatc
     7861 accaaagcac gcaaatctgt tgcaggcttc aaaatccgtc agggctatcc gatcggctgt
     7921 aaagtaactc tgcgtggcga acgcatgtgg gagttctttg agcgcctgat cactattgct
     7981 gtacctcgta tccgtgactt ccgtggcctg tccgctaagt ctttcgacgg tcgtggtaac
     8041 tacagcatgg gtgtccgtga gcagatcatc ttcccagaaa tcgactacga taaagtcgac
     8101 cgcgttcgtg gtttggatat taccattacc actactgcga aatctgacga agaaggccgc
     8161 gctctgctgg ctgcctttga cttcccgttc cgcaagtaag gtagggttac taaatggcta
     8221 agcaatcaat gaaagcacgc gaagtaaaac gcgtagcttt agctgataaa tacttcgcga
     8281 aacgcgctga actgaaagcg atcatctctg atgtgaacgc ttccgacgaa gatcgttgga
     8341 acgctgttct caagctgcag actctgccgc gtgattccag cccgtctcgt cagcgtaacc
     8401 gctgccgtca aacaggtcgt ccgcatggtt tcctgcggaa gttcgggttg agccgtatta
     8461 aggtccgtga agccgctatg cgcggtgaaa tcccgggtct gaaaaaggct agctggtaat
     8521 tgtcaccaat tgaatcacgg gagtaaagac agatgagcat gcaagatccg atcgcggata
     8581 tgctgacccg tatccgtaac ggtcaggccg cgaacaaagc tgcggtcacc atgccttcct
     8641 ccaagctgaa agtggcaatc gccaacgtgc tgaaggaaga aggttttatt gaagatttta
     8701 aagttgaagg cgacaccaag cctgaactgg aacttactct gaagtatttc cagggcaaag
     8761 ctgttgtaga aagcattcag cgtgtcagcc gcccaggtct gcgcatctat aaacgtaaag
     8821 atgagctgcc gaaagttatg gcgggtctgg gtatcgcagt tgtttctacc tctaaaggtg
     8881 ttatgactga tcgtgcagcg cgccaggctg gtcttggtgg cgaaattatc tgctacgtag
     8941 cctaatcgga ggaaaaaatg tctcgtgttg ctaaagcacc ggtcgttgtt cctgccggcg
     9001 ttgacgtaaa aatcaacggt caggttatta cgatcaaagg taaaaacggc gagctgactc
     9061 gtactctcaa cgatgctgtt gaagttaaac atgcagataa taccctgacc ttcggtccgc
     9121 gtgatggtta cgcagacggt tgggcacagg ctggtaccgc gcgtgccctg ctgaactcaa
     9181 tggttatcgg tgttaccgaa ggcttcacta agaagctgca gctggttggt gtaggttacc
     9241 gtgcagcggt taaaggcaat gtgattaacc tgtctctggg tttctctcat cctgttgacc
     9301 atcagctgcc tgcgggtatc actgctgaat gtccgactca gactgaaatc gtgctgaaag
     9361 gcgctgataa gcaggtgatc ggccaggttg cagcggatct gcgcgcctac cgtcgtcctg
     9421 agccttacaa aggcaagggt gttcgttacg ccgacgaagt cgtgcgtacc aaagaggcta
     9481 agaagaagta aggtaacact atggataaga aatctgctcg tatccgtcgt gcgacccgcg
     9541 cacgccgcaa gctccaggag ctgggcgcaa ctcgcctggt ggtacatcgt accccgcgtc
     9601 acatttacgc acaggtaatt gcaccgaacg gttctgaagt tctggtagct gcttctactg
     9661 tagaaaaagc tatcgctgaa caactgaagt acaccggtaa caaagacgcg gctgcagctg
     9721 tgggtaaagc tgtcgctgaa cgcgctctgg aaaaaggcat caaagatgta tcctttgacc
     9781 gttccgggtt ccaatatcat ggtcgtgtcc aggcactggc agatgctgcc cgtgaagctg
     9841 gccttcagtt ctaaggtaga ggtgtaagat ggctcacatc gaaaaacaag ctggcgaact
     9901 gcaggaaaag ctgatcgcgg taaaccgcgt atctaaaacc gttaaaggtg gtcgtatttt
     9961 ctccttcaca gctctgactg tagttggcga tggtaacggt cgcgttggtt ttggttacgg
    10021 taaagcgcgt gaagttccag cagcgatcca gaaagcgatg gaaaaagccc gtcgcaatat
    10081 gattaacgtc gcgctgaata acggcactct acaacaccct gttaaaggtg ttcacacggg
    10141 ttctcgcgta ttcatgcagc cggcttccga aggtaccggt atcatcgccg gtggtgcaat
    10201 gcgcgccgtt ctggaagtcg ctggggttca taacgttctg gctaaagcat atggttccac
    10261 caacccgatc aacgtggttc gtgcaactat tgatggcctg gaaaatatga attctccaga
    10321 aatggtcgct gccaagcgtg gtaaatccgt tgaagaaatt ctggggaaat aaaccatggc
    10381 aaagactatt aaaattactc aaacccgcag tgcaatcggt cgtctgccga aacacaaggc
    10441 aacgctgctt ggcttgggtc tgcgtcgtat tggtcacacc gtagagcgcg aggatactcc
    10501 tgctattcgc ggtatgatca acgcggtttc cttcatggtt aaagttgagg agtaagagat
    10561 gcgtttaaat actctgtctc cggccgaagg ctccaaaaag gcgggtaaac gcctggggcg
    10621 tggtatcggt tctggcctcg gtaaaaccgg tggtcgtggt cacaaaggtc agaagtctcg
    10681 ttctggcggt ggcgtacgtc gcggtttcga gggtggtcag atgcctctgt accgtcgtct
    10741 gccgaaattc ggcttcactt ctcgtaaagc agcgattaca gccgaagttc gtctatctga
    10801 cctggctaaa gtagaaggcg gtgttgtaga cctgaacacg ctgaaagcgg ctaacattat
    10861 cggtatccag atcgagttcg cgaaagtgat cctggctggc gaagtaacga ctccggtaac
    10921 tgttcgtggc ctgcgtgtta ctaaaggcgc tcgtgctgct atcgaagctg ctggcggtaa
    10981 aatcgaggaa taagtagcag atggctaaac aaccgggatt agattttcaa agtgccaaag
    11041 gtggcttagg cgagctgaaa cgcagactgc tgtttgttat cggtgcgctg attgtgttcc
    11101 gtattggctc ttttattccg atccctggta ttgatgccgc tgtacttgcc aaactgcttg
    11161 agcaacagcg aggcaccatc attgagatgt ttaacatgtt ctctggtggt gctctcagcc
    11221 gtgcttctat ctttgctctg gggatcatgc cgtatatttc ggcgtcgatc attatccagc
    11281 tgctgacggt ggttcaccca acgttggcag aaattaagaa agaaggggag tctggtcgtc
    11341 gtaagatcag ccagtacacc cgttacggta ctctggtgct ggcaatattc cagtcgatcg
    11401 gtattgctac cggtctgccg aatatgcctg gtatgcaagg cctggtgatt aacccgggct
    11461 ttgcattcta cttcaccgct gttgtaagtc tggtcacagg aacgatgttc ctgatgtggt
    11521 tgggcgaaca gatcactgaa cgaggtatcg gcaacggtat ttcaatcatt atcttcgccg
    11581 gtattgtcgc gggactcccg ccagccattg cccatactat cgagcaagcg cgtcaaggcg
    11641 acctgcactt cctcgtgttg ctgttggttg cagtattagt atttgcagtg acgttctttg
    11701 ttgtgtttgt tgagcgtggt caacgccgca ttgtggtaaa ctacgcgaaa cgtcagcaag
    11761 gtcgtcgtgt ctatgctgca cagagcacac atttaccgct gaaagtgaat atggcggggg
    11821 taatcccggc aatcttcgct tccagtatta ttctgttccc ggcgaccatc gcgtcatggt
    11881 tcgggggcgg tactggttgg aactggctga caacaatttc gctgtatttg cagcctgggc
    11941 aaccgcttta tgtgttactc tatgcgtctg caatcatctt cttctgtttc ttctacacgg
    12001 cgttggtttt caacccgcgt gaaacagcag ataacctgaa gaagtccggt gcatttgtac
    12061 caggaattcg tccgggagag caaacggcga agtatatcga taaagtaatg acccgcctga
    12121 ccttggttgg tgcgctgtat attaccttta tctgcctgat cccggagttc atgcgtgatg
    12181 caatgaaagt accgttctac ttcggtggga cctcactgct tatcgttgtt gtcgtgatta
    12241 tggactttat ggctcaagtg caaactctga tgatgtccag tcagtatgag tctgcattga
    12301 agaaggcgaa cctgaaaggc tacggccgat aattggtcgc ccgagaagtt acggagagta
    12361 aaaatgaaag ttcgtgcttc cgtcaagaaa ttatgccgta actgcaaaat cgttaagcgt
    12421 gatggtgtca tccgtgtgat ttgcagtgcc gagccgaagc ataaacagcg ccaaggctga
    12481 ttttttcgca tatttttctt gcaaagttgg gttgagctgg ctagattagc cagccaatct
    12541 tttgtatgtc tgtgcgtttc catttgagta tcctgaaaac gggcttttca gcatggaacg
    12601 tacatattaa atagtaggag tgcatagtgg cccgtatagc aggcattaac attcctgatc
    12661 ataagcatgc cgtaatcgca ttaacttcga tttatggcgt cggcaagacc cgttctaaag
    12721 ccatcctggc tgcagcgggt atcgctgaag atgttaagat cagtgagctg tctgaaggac
    12781 aaatcgacac gctgcgtgac gaagttgcca aatttgtcgt tgaaggtgat ctgcgccgtg
    12841 aaatcagcat gagcatcaag cgcctgatgg atcttggttg ctatcgcggt ttgcgtcatc
    12901 gtcgtggtct cccggttcgc ggtcagcgta ccaagaccaa cgcacgtacc cgtaagggtc
    12961 cgcgcaaacc gatcaagaaa taatcggggt gattgaataa tggcaaaggc accaattcgt
    13021 gcacgtaaac gtgtaagaaa acaagtctct gacggcgtgg ctcatatcca tgcttctttc
    13081 aacaacacca tcgtgactat cactgatcgt cagggtaacg cgttgggttg ggcaacagcc
    13141 ggtggttccg gtttccgtgg ttctcgcaaa tccactccgt ttgcagctca ggttgcagca
    13201 gagcgttgcg ctgacgccgt gaaagaatac ggcatcaaga atctggaagt tatggttaaa
    13261 ggtccgggtc caggccgcga atctactatt cgtgctctga acgccgcagg tttccgcatc
    13321 actaacatta ctgatgtgac tccgatccct cataacggtt gtcgtccgcc gaaaaaacgt
    13381 cgcgtataac gcctcgtttt ccaggtttgt tggagaaaga aaatggcaag atatttgggt
    13441 cctaagctca agctgagccg tcgtgagggc accgacttat tccttaagtc tggcgttcgc
    13501 gcgatcgata ccaagtgtaa aattgaacaa gctcctggcc agcacggtgc gcgtaaaccg
    13561 cgtctgtctg actatggtgt gcagttgcgt gaaaagcaaa aagttcgccg tatctatggt
    13621 gtgctggagc gtcagttccg taactactac aaagaagcag cacgtctgaa aggcaacacc
    13681 ggtgaaaacc tgttggctct gctggaaggt cgtctggaca acgttgtata ccgtatgggc
    13741 ttcggtgcca ctcgtgcaga agcacgtcag ctggttagcc ataaagcaat tatggtaaac
    13801 ggtcgtgttg ttaacatcgc ttcttatcag gttagtccga atgacgttgt aagcattcgt
    13861 gagaaagcga agaagcagtc tcgcgtgaaa gccgctctgg agctggctga gcagcgtgaa
    13921 aagccaactt ggctggaagt tgatgctggc aagatggaag gtacgtttaa gcgtaagccg
    13981 gagcgttctg atctgtctgc ggacattaac gaacacctga tcgtcgagct ttactccaag
    14041 taaagcttag taccaaagag aggacacaat gcagggttct gtgacagagt ttctaaaacc
    14101 gcgcctggtt gatatcgagc aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt
    14161 agagcgtggc tttggccata ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat
    14221 gccgggttgc gcggtgaccg aggttgagat tgatggtgta ctacatgagt acagcaccaa
    14281 agaaggcgtt caggaagata tcctggaaat cctgctcaac ctgaaagggc tggcggtgag
    14341 agttcagggc aaagatgaag ttattcttac cttgaataaa tctggcattg gccctgtgac
    14401 tgcagccgat atcacccacg acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg
    14461 ccacctgacc gatgagaacg cgtctattag catgcgtatc aaagttcagc gcggtcgtgg
    14521 ttatgtgccg gcttctaccc gaattcattc ggaagaagat gagcgcccaa tcggccgtct
    14581 gctggtcgac gcatgctaca gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg
    14641 tgtagaacag cgtaccgacc tggacaagct ggtcatcgaa atggaaacca acggcacaat
    14701 cgatcctgaa gaggcgattc gtcgtgcggc aaccattctg gctgaacaac tggaagcttt
    14761 cgttgactta cgtgatgtac gtcagcctga agtgaaagaa gagaaaccag agttcgatcc
    14821 gatcctgctg cgccctgttg acgatctgga attgactgtc cgctctgcta actgccttaa
    14881 agcagaagct atccactata tcggtgatct ggtacagcgt accgaggttg agctccttaa
    14941 aacgcctaac cttggtaaaa aatctcttac tgagattaaa gacgtgctgg cttcccgtgg
    15001 actgtctctg ggcatgcgct tggaaaactg gccaccggca agcatcgctg acgagtaacc
    15061 ggatcacagg ttaaggtttt actgagaagg ataaggtcat gcgccatcgt aagagtggtc
    15121 gtcaactgaa ccgcaacagc agccatcgcc aggctatgtt ccgcaatatg gcaggttcac
    15181 tggttcgtca tgaaatcatc aagacgactc tgcctaaagc gaaagagctg cgccgcgtag
    15241 ttgagccgct gattactctt gccaagactg atagcgttgc taatcgtcgt ctggcattcg
    15301 cccgtactcg tgataacgag atcgtggcaa aactgtttaa cgaactgggc ccgcgtttcg
    15361 cgagccgtgc cggtggttac actcgtattc tgaagtgtgg cttccgtgca ggcgacaacg
    15421 cgccgatggc ttacatcgag ctggttgatc gttcagagaa agcagaagct gctgcagagt
    15481 aatctgaagc aacgtaaaaa aacccgcccc ggcgggtttt tttatacccg tagtatcccc
    15541 acttatctac aatagctgta ctctttt
//
LOCUS       DADMFL010000092        15027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.093, whole genome shotgun sequence.
ACCESSION   DADMFL010000092 DADMFL010000000
VERSION     DADMFL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.093"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..236
                     /locus_tag="JNZ27_002957"
     CDS             <1..236
                     /locus_tag="JNZ27_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY8665213.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="JNZ27_002958"
     CDS             246..884
                     /locus_tag="JNZ27_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY8665214.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNLYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWLPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="JNZ27_002959"
     CDS             887..2050
                     /locus_tag="JNZ27_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8665215.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3758
                     /locus_tag="JNZ27_002960"
                     /pseudo
     CDS             2134..3758
                     /locus_tag="JNZ27_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="JNZ27_002961"
     CDS             complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="JNZ27_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY8665216.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="JNZ27_002962"
     CDS             complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="JNZ27_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY8665217.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4810..5559
                     /gene="yjfP"
                     /locus_tag="JNZ27_002963"
     CDS             4810..5559
                     /gene="yjfP"
                     /locus_tag="JNZ27_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8665218.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="JNZ27_002964"
     CDS             complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="JNZ27_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY8665219.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="JNZ27_002965"
     CDS             complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="JNZ27_002965"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY8665220.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7838..9235
                     /gene="ulaA"
                     /locus_tag="JNZ27_002966"
     CDS             7838..9235
                     /gene="ulaA"
                     /locus_tag="JNZ27_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY8665221.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9251..9556
                     /gene="ulaB"
                     /locus_tag="JNZ27_002967"
     CDS             9251..9556
                     /gene="ulaB"
                     /locus_tag="JNZ27_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY8665222.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9566..10030
                     /gene="ulaC"
                     /locus_tag="JNZ27_002968"
     CDS             9566..10030
                     /gene="ulaC"
                     /locus_tag="JNZ27_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY8665223.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10044..10694
                     /gene="ulaD"
                     /locus_tag="JNZ27_002969"
     CDS             10044..10694
                     /gene="ulaD"
                     /locus_tag="JNZ27_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY8665224.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10704..11540
                     /locus_tag="JNZ27_002970"
     CDS             10704..11540
                     /locus_tag="JNZ27_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY8665225.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11540..12226
                     /locus_tag="JNZ27_002971"
     CDS             11540..12226
                     /locus_tag="JNZ27_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY8665226.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYIFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12355..12630)
                     /locus_tag="JNZ27_002972"
     CDS             complement(12355..12630)
                     /locus_tag="JNZ27_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8665227.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12957..13352
                     /gene="rpsF"
                     /locus_tag="JNZ27_002973"
     CDS             12957..13352
                     /gene="rpsF"
                     /locus_tag="JNZ27_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY8665228.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13359..13673
                     /gene="priB"
                     /locus_tag="JNZ27_002974"
     CDS             13359..13673
                     /gene="priB"
                     /locus_tag="JNZ27_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY8665229.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13678..13905
                     /gene="rpsR"
                     /locus_tag="JNZ27_002975"
     CDS             13678..13905
                     /gene="rpsR"
                     /locus_tag="JNZ27_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY8665230.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13947..14396
                     /gene="rplI"
                     /locus_tag="JNZ27_002976"
     CDS             13947..14396
                     /gene="rplI"
                     /locus_tag="JNZ27_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY8665231.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14467..15024)
                     /locus_tag="JNZ27_002977"
     CDS             complement(14467..15024)
                     /locus_tag="JNZ27_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8665232.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3509 a         3663 c         4051 g         3804 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatctctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggctcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccatggat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag gttgaggctg
     2521 ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2581 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2641 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2701 gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc tcttatcggc
     2761 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2821 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2881 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     2941 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3001 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3061 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3121 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3181 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3241 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3301 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3361 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt tccggcaata
     3421 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
     3481 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
     3541 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
     3601 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
     3661 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
     3721 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
     3781 tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg cgctcgcgcc
     3841 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
     3901 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
     3961 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
     4021 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
     4081 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
     4141 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
     4201 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
     4261 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
     4321 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
     4381 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     4441 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     4501 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     4561 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     4621 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     4681 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     4741 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     4801 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     4861 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     4921 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     4981 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     5041 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     5101 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     5161 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     5221 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     5281 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     5341 acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     5401 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     5461 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     5521 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     5581 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     5641 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     5701 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     5761 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     5821 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     5881 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     5941 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     6001 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     6061 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     6121 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     6181 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     6241 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     6301 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     6361 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     6421 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     6481 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     6541 agataaacgg cttgaagcca tacttcagac gatctttttt catctcccac agcacgcgga
     6601 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     6661 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     6721 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     6781 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     6841 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     6901 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     6961 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     7021 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcatcagcac
     7081 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     7141 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     7201 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     7261 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     7321 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     7381 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     7441 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     7501 atagttcaat tcgaatcaat atgtgattgg ttttgattaa tcctgacact attttttcag
     7561 gaaggcaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgtag
     7621 aaagatttaa tggaatgtat agataaatta actaaatgaa tttaaataaa taattttttc
     7681 gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgtca catttaatca
     7741 taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca ttccgggtgt
     7801 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     7861 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     7921 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     7981 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     8041 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     8101 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     8161 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     8221 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     8281 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     8341 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     8401 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     8461 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     8521 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     8581 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     8641 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     8701 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     8761 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     8821 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     8881 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     8941 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     9001 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     9061 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     9121 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     9181 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     9241 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     9301 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     9361 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     9421 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     9481 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     9541 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     9601 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     9661 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     9721 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     9781 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     9841 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggtggtcg atgccaacac
     9901 gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     9961 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
    10021 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
    10081 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
    10141 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
    10201 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
    10261 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
    10321 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
    10381 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
    10441 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata
    10501 tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg
    10561 cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc gccggtcgca
    10621 gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg
    10681 aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc atctatgaaa
    10741 aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa acgttgggct
    10801 tcgattttgt cgagatgtcg gtagatgaaa ctgacgagcg cctgtcgcgc ctcgactgga
    10861 gccgcgagca gcgtctggcg ctggtgaatg cgattgttga aaccggcgtg cgtgtgccgt
    10921 ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg
    10981 cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg
    11041 tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa acgcgtcgtc
    11101 gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcacag gtgacgctgg
    11161 cgatggagat catggattat ccgttgatga actccatcag caaggcgctg ggttacgcgc
    11221 actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg tcggcgtggg
    11281 acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg gtgcatgtga
    11341 aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg gtggatttcg
    11401 aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg attgagatgt
    11461 ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgcgcatg gccaaagcag
    11521 gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag ccaacatgga
    11581 tctgccgcgc tatgggctgg tgacctttac ttggggcaac gtcagcgcta tcgaccgcga
    11641 acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcagacga
    11701 tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc catcttccga
    11761 cactgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca ttgtccatac
    11821 ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg cattaggcac
    11881 cacgcacgct gactacatct ttggtgatat cccgtgtacg cgtggattaa gcgaagaaga
    11941 ggtgcagggt gagtatgaac tgaacactgg caaagtgatt atcgaaacgc tgggcaacgc
    12001 cgagccgctg catacgctgg gaattgtggt gtatcagcac ggaccgttcg cctgggggaa
    12061 agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga aaatggcgtg
    12121 gattgcgcgc agtattaatc cacaactcaa tcatatcgac agctacctga tgaataaaca
    12181 cttcatgcgt aagcacggtc ctaacgctta ttacgggcag aagtaaaaca cgcgctgcgg
    12241 aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca aaaaaagccc
    12301 cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg
    12361 ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac
    12421 gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac gtgcttcgct
    12481 ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat cgtccatatt
    12541 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
    12601 agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg actttgtgtg
    12661 ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga
    12721 ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga atgtgatgaa cttcaaatca
    12781 gcggtttaga ggttatttgc gaaaggggag atttatttct gctccgccct tgagtttagc
    12841 ggggtatgtc agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct
    12901 ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc
    12961 gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga
    13021 tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact
    13081 ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga
    13141 tgaacattga agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg
    13201 atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga
    13261 tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg
    13321 atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt
    13381 ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc
    13441 agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg caggcatggt
    13501 gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg
    13561 tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga
    13621 gcaaaatggt tttgcacgcc gagcagattg aattgataga ttctggagac tagccatatg
    13681 gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc
    13741 gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca
    13801 agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc
    13861 gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat
    13921 taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc
    13981 tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac
    14041 cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg
    14101 ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa
    14161 tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg
    14221 gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta
    14281 agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac gaagtgagct
    14341 tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg
    14401 ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc tatttttcgt
    14461 caggcattaa tttcgcaagt agatccattc accattctct tgtgtccata agtcaggatg
    14521 gtttgctggc gttagatgca tacgatcact ccttatgtct cggtcctgcg gcactcgctc
    14581 gaaacctaaa cccgagtaga ttgcggctga atttgataag gaacgtagac caattttcaa
    14641 atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc cgagggcatt
    14701 agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg gtagttcaga
    14761 tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg tttggccggt
    14821 atagtctgga gtactataac caggtcccaa aacaacctcg tgtattgtgt atttactgtt
    14881 gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta acctcgcacc
    14941 cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt catggttcca
    15001 tccaaaagaa ttatcaccat tcattgt
//
LOCUS       DADMFL010000093        14925 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.094, whole genome shotgun sequence.
ACCESSION   DADMFL010000093 DADMFL010000000
VERSION     DADMFL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.094"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            597..794
                     /locus_tag="JNZ27_002978"
     CDS             597..794
                     /locus_tag="JNZ27_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665233.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JNZ27_002979"
     CDS             complement(744..1937)
                     /locus_tag="JNZ27_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8665234.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JNZ27_002980"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JNZ27_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY8665235.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JNZ27_002981"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JNZ27_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY8665236.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JNZ27_002982"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JNZ27_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY8665237.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="JNZ27_002983"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="JNZ27_002983"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY8665238.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="JNZ27_002984"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="JNZ27_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY8665239.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="JNZ27_002985"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="JNZ27_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY8665240.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFEDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIDDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLALFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3554 c         3707 g         3644 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcagaccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggt aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttactg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactctaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgtttg aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccatagatg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgcc
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat
    14881 gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADMFL010000094        14787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.095, whole genome shotgun sequence.
ACCESSION   DADMFL010000094 DADMFL010000000
VERSION     DADMFL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.095"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..157
                     /locus_tag="JNZ27_002986"
     CDS             <1..157
                     /locus_tag="JNZ27_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8665241.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JNZ27_002987"
     CDS             227..1033
                     /locus_tag="JNZ27_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8665242.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JNZ27_002988"
     CDS             1043..1450
                     /locus_tag="JNZ27_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY8665243.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JNZ27_002989"
     CDS             1450..1944
                     /locus_tag="JNZ27_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY8665244.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JNZ27_002990"
     CDS             2010..2807
                     /locus_tag="JNZ27_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8665245.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JNZ27_002991"
     CDS             2818..3606
                     /locus_tag="JNZ27_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY8665246.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JNZ27_002992"
     CDS             3658..4887
                     /locus_tag="JNZ27_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8665247.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JNZ27_002993"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JNZ27_002993"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY8665248.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JNZ27_002994"
                     /pseudo
     CDS             complement(6049..7680)
                     /locus_tag="JNZ27_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNZ27_002995"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNZ27_002995"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY8665249.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JNZ27_002996"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JNZ27_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY8665250.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..14787
                     /locus_tag="JNZ27_002997"
                     /pseudo
     CDS             12924..14787
                     /locus_tag="JNZ27_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT         3679 a         3783 c         3710 g         3615 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatctgcag gataaaatca ttattgtcac cggcggcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttactc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa ctaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tctttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaa
//
LOCUS       DADMFL010000095        14683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.096, whole genome shotgun sequence.
ACCESSION   DADMFL010000095 DADMFL010000000
VERSION     DADMFL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.096"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JNZ27_002998"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JNZ27_002998"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY8665251.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JNZ27_002999"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JNZ27_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY8665252.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNZ27_003000"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNZ27_003000"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY8665253.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JNZ27_003001"
     CDS             complement(3965..5611)
                     /locus_tag="JNZ27_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY8665254.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JNZ27_003002"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JNZ27_003002"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY8665255.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JNZ27_003003"
     CDS             7086..8594
                     /locus_tag="JNZ27_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8665256.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8819..10084)
                     /gene="uidC"
                     /locus_tag="JNZ27_003004"
     CDS             complement(8819..10084)
                     /gene="uidC"
                     /locus_tag="JNZ27_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY8665257.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10123..11403)
                     /gene="uidB"
                     /locus_tag="JNZ27_003005"
                     /pseudo
     CDS             complement(10123..11403)
                     /gene="uidB"
                     /locus_tag="JNZ27_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445901.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11492..13303)
                     /gene="uidA"
                     /locus_tag="JNZ27_003006"
     CDS             complement(11492..13303)
                     /gene="uidA"
                     /locus_tag="JNZ27_003006"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY8665258.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13691..14281)
                     /locus_tag="JNZ27_003007"
     CDS             complement(13691..14281)
                     /locus_tag="JNZ27_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8665259.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14510..>14683)
                     /locus_tag="JNZ27_003008"
     CDS             complement(14510..>14683)
                     /locus_tag="JNZ27_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8665260.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3681 a         3822 c         3496 g         3684 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aacgcgcact ttgtcaacaa tctgaaacgc cggaggtttt tctctccggc gttatttctt
     8821 acttcagcat aaagtcatac tgaatactca ttttcacatc ccagtaattc aggcgatcgc
     8881 ccagcgtact gccatcgcgc ttttcccgcc ctttatcggc agcaagaaag aaattcatcc
     8941 cttgtaacgg cccttcgctg acccgatagt tcataccacc taagataccc gcgccttcaa
     9001 tccgcacatc tcgtttactg gattcatagc cgcgagtcac aatcggtgca aatgtcagcg
     9061 taaattgcgg agttacacgg tagttgacac ctaattgcca ggactgcatt ccttcacggt
     9121 tacgatcaag acttaatgaa aagggatagc caatgtcggt atccaccacg gcaccattca
     9181 ttgcatggcg caaataacca ccactgccaa atacgcttcc ccacggcgca ttccaggtca
     9241 attgtccact aaccaacgcc gtttcattgg gctggctggt attacgactt aaaccttcga
     9301 gctgcgcgta aaagccgagc aactcccctt tcaacgtata attcgggtcg agcggtatgc
     9361 ttttctcgag aatactttgt atctgattgg aaacatcttt ctgtaccgat gcccccagag
     9421 aatagcgact ggtatcatca tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat
     9481 cgtattttcc cgtctggctg ttatacaccc cttcatcacg aataccggtt gttgaatcag
     9541 tccataaaaa tactcgtgaa cgccacattc cccacagatc aatatttggt gttaaagccc
     9601 aataacctaa tgctccttca gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta
     9661 acggaatacg ataaggtaag ctaccataac tataatccgt cccgaagcga cctaatttaa
     9721 gcaggctatt ttcactgggt tttattttta ctgcccccag ggcaaagcca aaacttttat
     9781 caccatcaag ataccaccgg gtactcatat cagcacgagc acctaattta taaacataat
     9841 acgctccgcc ttcaacgcca ataatattgg aataataacc actattgaaa tcgagcaatc
     9901 ctccctgtac ccatgcgtaa atatctcttc ccgcgccagc actgggctta tcagccctgc
     9961 gcaattcgtt tttcagtctg atgcgtagtc ccgcttcatc gtcagccagt tgcgccacgt
    10021 aagcagaggt aaggccagag gcagccgtca ggcaaataac ggccatggcc actatttttc
    10081 tcatagttgc acctttgatg ttctgattat ttttattgaa tattaattag tgatatcgct
    10141 gattaattgc tgctgcactt ttttacgatt atcaatttca accacgattt ctttgaattt
    10201 tttatccgtg agcggataaa accagataat aacgaatgcc agtagcataa atccgcaagg
    10261 tactaaggca attgatgtgc ggatgcccat aataacttcc ggcgtttaca cctgattggc
    10321 gatatatccg cttaatccca aaataaaggc aggaattgaa cctccgattg cctgaccgca
    10381 tttacgggta aatgagaata gtgaataggt gagcccttca attcgcacgc cggtcaggta
    10441 ttcaccgtat tctacggtat cagcttccag tgcccacatc acagtcatag taacgccctg
    10501 accaattgaa gcgatggcca acgcaaccaa cgccaccggc agtgaccaga cggaaaccca
    10561 gaagaatagc agataaccgc aggttcccag caaagcgcca atcaggaagg tattcttttt
    10621 accgatcctc gcgaccatcc ccggcacaag cggtgccgat gccacagtac caaccaggtt
    10681 ttgcaccagt accagcacag tgaacagccc ggtatcattt aacacatagc gcacgtagaa
    10741 caacgacgag gcgctgaccg cgaaggtcga aatcagcaca cacagcgcac cgatgcacaa
    10801 cataaacagc gggcgattcc gtttcagggt ttgcaaactg atcttcaatg acggctgcgc
    10861 cacgatacgt accacatttt cacgcgtcga tttgaagcag atgaagtaaa gcaccattcc
    10921 ggcaatcgcc agcacaatcg tccagaaatg gtataccgac accatctctt ccgggctgga
    10981 gttcttaatg ctcggtccta tcagaaatgc caggcataca aaggtcaatg aagcggcaat
    11041 cccacgagcc gcgcccagac gggcgcgaga ttgtggttgt tgggtcatcg cggtagcaag
    11101 tgaaccataa ggaatattca ccaggctgta gcaaagcccg aggcccatgt aggtcaaata
    11161 tgcatacacc actttgctac catggctcca gtcggtcggt acccagaata ccagcacgct
    11221 gaagatcatt aacggcgcag taccgaagag taaaaacggg cggaattttc cccagcgggt
    11281 attcacactg tccaccactc gtccggcaaa gacgtcggcg aaggcatcga atacccgcac
    11341 cagtaacagc atggtgcctg ccgcagcggc accgacgcca gcgacgtcgg tgtagtaact
    11401 caataggaag acgcccccat tgcgaaggcg aagttattgg cgacgtcacc gaggctgtag
    11461 ccgacgatgg tgcgccagga gagttgttga ttcattgttt gcctccctgc tgcggttttt
    11521 caccgaagtt catgccagtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc
    11581 ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg caatatgcct tgcgaggtcg
    11641 caaaatcggc gaaattccat acctgttcac cgacgacggc gctgacgcga tcaaagacgc
    11701 ggtgatacat atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg
    11761 agtgcaggcc ggctaacgta tccacgccgt attcggtgat gataatcggc tgatgcagtt
    11821 tctcctgcca ggccagcagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc
    11881 tttggacata ccaaccgtaa taacggttca ggcacagcac atcaaagaga tcgctgatgg
    11941 tatcggtgtg agcgtcgcag aacattacat tgacgcaggt gatcggacgc gtcgggtcga
    12001 gtttacgcgt tgcttccgcc agtggcgcga aatattcccg tgcaccttgc ggacgggtat
    12061 ccggttcgtt ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgctatca
    12121 gctctttaat cgcctgtaag tgcgcctgct gagtttcccc gttgacagcc tcttcgctgt
    12181 acagttcttt cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga
    12241 cagctgcagt ttcatcaatc accacgatgc catgttcatc tgcccagtcg agcatctctt
    12301 cagcgtaagg gtaatgcgag gtacggtagg agttggcccc aatccagtcc attaatgcgt
    12361 gatcgtgcac catcagcacg ttatcgaatc ctttgcctcg caaatccgca tcttcatgac
    12421 gaccaaagcc agtaaagtag aacggtttgt ggttaatcag gaactgttcg cccttcactg
    12481 ccactgaccg gatgccgacg cgcagcgggt agatatcaca ctctgtctgg cttttggctg
    12541 tgacgcacag ttcatagaga taaccttcac ccggttgcca gaggtgcgga ttcaccactt
    12601 gcaaagtccc gctggtgcct tgtccagttg caaccacctg ttgatccgca tcacgcagtt
    12661 caacgctgat atcaccattg gccaccacct gccagtcaac agacgcgtgg ttacagtctt
    12721 gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg gtgtagagca
    12781 ttacgctgcg atggatcccg gcatagttaa agaaaccatg gaagtaagac tgctttttct
    12841 tgccgttttc gtcggtaatc accattcccg gcgggatagt ctgccagttc agttcgttgt
    12901 tcacacaaac ggtgatacgt acacttttcc cggcaataac atacggcgtg acatcggctt
    12961 caaatggcgt atagccgccc tgatgctcca tcacttcctg attattgacc cacactttgc
    13021 cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg gcctgcccaa cctttcggta
    13081 taaagacttc gcgctgatac cagacgttgc ccacataatt acgaatatct gcatcggcga
    13141 actgatcgtt aaaactgcct ggcactgcaa ttgcccggct ttcttgtaac gcgctttccc
    13201 accaacgctg atcaattcca cagttttcgc gatccagact gaatgcccac aggccgtcga
    13261 gttttttgat ttcacgggtt ggggtttcta caggacgtaa cataagggac tcctcattaa
    13321 gataaaaata ctggtcaacc tttaatctga ttagttaacc aattctgtgt tgcacacagt
    13381 tatctgtgag aaacccatgg aagagaaatg gaaataaaaa gaactgacac cgcgatcaaa
    13441 aaaccaaaaa ctgcaatacc cacagcgtga tatggatcgc attaatcttt aaaacagcgt
    13501 tgacagaagc cataaaacaa gcaatcgagc gttcgttcga gagtattggg aacgataaac
    13561 tctcatgaca tatggctacg gtgaatattt tgggggggaa ttctccgaac acggggagga
    13621 taatttttgc acagagaaat tacgatatga aacgcgggca cagttatcac cagcccgcga
    13681 aatgagagga tcaggatgcg gttaatatac cgccaatcat cgcgcgtaat ccctgagcaa
    13741 acgacgcttc acgcgccagg tcttccgctt cgatatcggc cagtacaccg taggtcagat
    13801 ccagcagtaa acgtgaagtc attgccgtgt tgatgtctgg gcttatctcg cctttttgct
    13861 gggcatcacg catccgctgg gcaacaaatt cagtgatcgt catatgctta tttttaagca
    13921 tggcggcgac ctgtgggtta cgcatccctt ccgccataat ttcgaccacc agcgcccgtt
    13981 gcccaaaggc ttcatgggtg agagagacaa tagactcgac catatagtca acaaaatgaa
    14041 tcccttcaat cggttcccgg aaacgagcca gcgccctctc ctggtcctgt aagataatcg
    14101 cctgaatcaa ggcttctttg gagatgaaat ggtgatagag cgtcccagga ctaatggcgc
    14161 aagatttaca gatggctttc atcgaggcac tgtgaaatcc attttctgaa aaaatctctc
    14221 tggcagcatt gaggatccgg gtccgtgtcg gttgtgcttc agtctgcatg ttatccatca
    14281 ttaaaccatt atagttagcg tgaataacta gtttaccgcg ttgtcaggtg agcgcctatc
    14341 attgctgttg aatagtgcga aggtacactc tattcgtata cttttcaccc agcaattggt
    14401 taaccaattg ccgtgatttg gctgccagtt gtttagcaga gacgttgatg aattgcacac
    14461 gctatcccgg taaacaacac atacgaccgt ttaccggttc gttagtgtat taattgagct
    14521 cctgtacccc accaccggag acggtgagaa tttgtccgct tacccagctg gctgcaggcg
    14581 agcaaaggaa cagcgccgcg ttagcaatat cttgcggttg gcccagacgt ctgatcggcg
    14641 tgtgctgtaa cattttttgt tcaatttctg ggtaatgact cca
//
LOCUS       DADMFL010000096        14363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.097, whole genome shotgun sequence.
ACCESSION   DADMFL010000096 DADMFL010000000
VERSION     DADMFL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.097"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..454
                     /locus_tag="JNZ27_003009"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNZ27_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JNZ27_003010"
     CDS             514..621
                     /locus_tag="JNZ27_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY8665261.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNZ27_003011"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNZ27_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY8665262.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNZ27_003012"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNZ27_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3388)
                     /gene="yehY"
                     /locus_tag="JNZ27_003013"
                     /pseudo
     CDS             complement(2257..3388)
                     /gene="yehY"
                     /locus_tag="JNZ27_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3395..4312)
                     /gene="osmF"
                     /locus_tag="JNZ27_003014"
     CDS             complement(3395..4312)
                     /gene="osmF"
                     /locus_tag="JNZ27_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY8665263.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4564..6861)
                     /gene="bglX"
                     /locus_tag="JNZ27_003015"
     CDS             complement(4564..6861)
                     /gene="bglX"
                     /locus_tag="JNZ27_003015"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY8665264.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7057..8772
                     /gene="dld"
                     /locus_tag="JNZ27_003016"
     CDS             7057..8772
                     /gene="dld"
                     /locus_tag="JNZ27_003016"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8665265.1"
                     /translation="MSSMTTTDNKAFLNELARLVSHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8810..9742)
                     /gene="pbpG"
                     /locus_tag="JNZ27_003017"
     CDS             complement(8810..9742)
                     /gene="pbpG"
                     /locus_tag="JNZ27_003017"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY8665266.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9916..10503)
                     /locus_tag="JNZ27_003018"
     CDS             complement(9916..10503)
                     /locus_tag="JNZ27_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY8665267.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10673..11251
                     /locus_tag="JNZ27_003019"
     CDS             10673..11251
                     /locus_tag="JNZ27_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8665268.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YSFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11368..12129)
                     /locus_tag="JNZ27_003020"
                     /pseudo
     CDS             complement(11368..12129)
                     /locus_tag="JNZ27_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12182..13708)
                     /gene="mdtQ"
                     /locus_tag="JNZ27_003021"
     CDS             complement(12182..13708)
                     /gene="mdtQ"
                     /locus_tag="JNZ27_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY8665269.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDVL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
     gene            13842..13925
                     /gene="yohP"
                     /locus_tag="JNZ27_003022"
     CDS             13842..13925
                     /gene="yohP"
                     /locus_tag="JNZ27_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY8665270.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(14296..>14363)
                     /locus_tag="JNZ27_003023"
     CDS             complement(14296..>14363)
                     /locus_tag="JNZ27_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY8665271.1"
                     /translation="RVLNNSPDIARAIQAIDIEKL"
BASE COUNT         3420 a         4023 c         3609 g         3311 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccaga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaaacacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cattttgcgc cagttgggtc gccgcctttc
     3121 cagctcccca caccaacaat acgaagacga attgcgccag aatgagtgca aaaatgctgc
     3181 cttttttagc ggggacaaaa caggctgtca gccaggcgca accaacgccc accagcatcc
     3241 agatcgtttg cggccacagt tgccagagat gaagcccttc acccgagact aaacgattag
     3301 gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacaac agcagcgcca
     3361 gaacaggatt aatacgtaga taagtcacgg gaaattactt cgcccaccct ttttgtttca
     3421 ggtagtcggc agccactttt ttggcatcca gtccttccac tgcaatgctg gcattcagtt
     3481 gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg
     3541 gatactcctt caacaccgat tcacgcacca ctggtgcagg cgcgtagata ggttgcacgc
     3601 cttgcggatc gcttaaggtt tgcagcccca gcgccgcgac cggagcgtca gtgccgtaag
     3661 ccattgcggc attaacgcca gaggtttgct gggcagcggc tttaatcgtc accgccgtgt
     3721 cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
     3781 acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc
     3841 cgccctcttg cagataacga ctcaggtcgg caagcgaagt gagtttgttt ttctctgcca
     3901 catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
     3961 acttgttgtg ctccgcatcg agctttttga ctttctcgta gccctgcccg gcatttttcc
     4021 acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga
     4081 tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt
     4141 tattcaccgt cggtacgccg tggctttcga gtacctgcaa gatgatgttg ccgagcagcg
     4201 cgccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc
     4261 tcacggctgc caacataacc agtgaacctg cccagagctt taagagtggc atgatgcttt
     4321 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
     4381 agttagcctg agcggaagga ttcagttgca gaatcagata aatacttaag aggcatattc
     4441 ggtccggcgt ttcctgccgg atgcggcgcg agcgccttat ccggcctaca aagggcgaaa
     4501 attcagcaag ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa
     4561 gcattacagc aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaatac
     4621 attgaacttg ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc
     4681 aatatcgatc gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa
     4741 gcctttcagc tgtttcacag ggcgactcat ggaagccgtc acatcctgca agtacatctg
     4801 cactaccgtg gccccctcgc gcttaccggt gttcgtcacc tgcacgctgg ccgtcacttt
     4861 gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata
     4921 gctcagacca tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt
     4981 gtatttgttc ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac
     5041 cgggatctgc ccgacagaac gcgggaagga cattggcagc ttgccggacg ggttgtaatc
     5101 gccaaacaat acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag
     5161 aatcgcatca gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac
     5221 cagcaccagc ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggccttgcgg
     5281 aatagtgata tcggtccggc tggaggcttc gtgcgccatc ccctgtgctt caccgactac
     5341 agccaccacc acatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg
     5401 cgaacgcgga tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt
     5461 gtcactggta acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggaatt
     5521 tttaatcccg gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc
     5581 catcacgtca cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt
     5641 cagcggtaac gtttcgagac ggtttttcag caacactagg ctttcgcgcg ccacttcacg
     5701 cgcttcttta cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcgg
     5761 ccccaaatgg ctgtatgggt cgttaaacaa ccccatatca tatttaacgt tcagtacatg
     5821 gcgggcagca tcgtccagct ctgccatggt cactttgccg gatttaatca acccaggcag
     5881 atacttcgag tagtactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg
     5941 caccgcatct tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg
     6001 atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca
     6061 ggaatcggag gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct
     6121 gcctgcgtcc agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact
     6181 catatcgacg gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt
     6241 gacgctggtc atcaccgagt aacgatctgc cgggctttta ccctgcatcg cttccaccat
     6301 ggttttaccc attattgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc
     6361 ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc
     6421 tgccgcttca taagcagaga cacgcccgac cgttttcacc gcatcgaggt taaaagacga
     6481 ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa
     6541 gaaaagagga attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc
     6601 ctgacgggtt acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat
     6661 cgcctctttc ggattatccg ggcctacgct gattaagcgc agctgaccaa ttttctcatc
     6721 aaccatcatt ttcttgagca gatcggtgac gaacgcatcc cgcgcttcag gcgttaatgg
     6781 atggttaccg aataaatcat ccgccagtgc tggctgcagg accagactca ccgcgattcc
     6841 tactgaacat agccatttca tatggatttt tcttcctttg ttgcccgacg tggcagcgaa
     6901 aatggtgcaa aaaccgtagt ttgccataag catgatggag agagaaaaag aatgctcagt
     6961 ttattgtctg aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag
     7021 tttgtgatat tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat
     7081 aataaagcct ttttgaatga acttgcccgt ctggtcagtc attcacacct gctcaccgat
     7141 cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct
     7201 gtcgttttcc ctggctcact actggaattg tggcgggtgc tgaaagcctg cgtcaccgcc
     7261 gacaaaatta ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac
     7321 ggtaacgact atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac
     7381 gttctcggca agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa
     7441 aaagccctca aaccgctggg acgcgaaccg cattcggtga ttggatcatc gtgtataggc
     7501 gcatcggtca tcggcggtat ttgtaacaac tccggcggct cgctggtgca acgtggcccg
     7561 gcgtataccg aaatgtcgtt attcgcgcgt atagatgaag atggcaaact gacgctggtg
     7621 aaccatctgg ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgac
     7681 gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat
     7741 gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatccggat
     7801 cggttatttg aatcttctgg ttgcgccggg aagctggccg tctttgcggt acgtcttgat
     7861 accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg
     7921 ctgaccgaaa tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa
     7981 tatatgcacc gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg
     8041 attgataagt taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat
     8101 gcgatgctgg agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc aatgcaaaaa
     8161 ttcggtcacc tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac
     8221 gagcatcatc tgctgttaaa aatggcaggc gatggcgtcg gtgaagccaa atcgtggctg
     8281 gtggattatt tcaaacaggc cgagggcgat ttctttgtct gtacgccgga ggaaggtagc
     8341 aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat
     8401 tccgatgaag tcgaagacat tctggcactg gatatcgctc tgcggcgtaa cgacaccgag
     8461 tggtatgagc atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc
     8521 cattttatgt gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat
     8581 gcgttaaaag aacagatgct ggaactgcta cagcagcgcg gcgcgcagta ccctgccgag
     8641 cataacgtcg gtcatttgta taaagcaccg gagacgttgc agaagttcta tcgcgagaac
     8701 gatccgacca atagcatgaa tccggggatc ggtaaaacca gtaaacggaa aaactggcag
     8761 gaagtggagt aaaaattacg gatggcagag tatcgccatc cgaattcact taatcgttct
     8821 gtgccgtctg ccccgccgcc gccatttggg ctgctttttg ttttttatag ctcaacgctg
     8881 ctgccggcac aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg
     8941 caaaatgggt atatttgcca aacgcgtcca tcactaccag cgccaccggt ttattattga
     9001 taaccgtacg catcaccaga caatggcccg ccgcattggt aaagccggtt ttggttaact
     9061 gaatattcca gttatcgcga tacaccagat gattagtatt gcggaacggc agcgtatacg
     9121 tcgggttaga gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat
     9181 attgtttgct ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg
     9241 acaatccggt aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat
     9301 tcattgcctt aataaaggct ttgtaaccac cgggataatg gtgcgcaagg cttgccgccg
     9361 cgcggttttc tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat
     9421 tcagtcgtac gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta
     9481 gtttttcatc cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga
     9541 tagacgcaat cggacgcacc agatccgggt ggttcgaata gatcactttg ttggtattca
     9601 gatcaacaat catcgcgcta ccggaggcaa tttccggttg tgaagcggtg gtagcggctg
     9661 ccgttttcgc aacggcctgc ggtgcaaaag gcacagccag catcaaggcc aggctaaata
     9721 aagaaactcg aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacgggttgc
     9781 gcaccgccgg agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc
     9841 gatcgtcaaa ggagaatcgt gagtaaatgc tgcattgctg acatttacgc cagcaatgca
     9901 acgtcaaaga actttctaga acaaacgata accgtagccc catagtataa cggttagggt
     9961 gagcagcact tccagtacca gcacgccaat cgccagcgtc gaactggaga agctaaggcc
    10021 ttcctctttg ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta
    10081 aaacagcgcc accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag
    10141 accgcttaaa aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagaga
    10201 cggacgctgc gggtaattgc gcgccatcca ccagatgacc cgccccatca ccgcaacccc
    10261 agccagcata acgccgtaaa acaagacagc cagcgccagt ccggtaaacc aggataactt
    10321 caggatagtg ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat
    10381 caccggaatc gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt
    10441 ttcgcgatta atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct
    10501 cataccgccc ccttgttgtg agttcatgaa cctgacagtt caagtataag ccagcttgtg
    10561 attatttttt gttcatccat gtaatttgca ccgtctgatg tatgtccttt ttcagcgtga
    10621 atgattcaca gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct
    10681 caatacactc atctcacaat atggttatgc cgcgctggtt atcggtagcc tagcggaagg
    10741 tgaaaccgtg actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccact
    10801 ggtggtactt tctgtggcgc ttggtggcat gattggcgac caggtgctct atctgtgcgg
    10861 gcggcggttt ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg
    10921 ggcgcagaaa cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta
    10981 tagctttcgg gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt
    11041 tctgccgctg aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta
    11101 cgctggtggt caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg
    11161 ggtctggttg attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg
    11221 cgggaagaaa aagccggata atcaggcgta aagcagttgc cacagataag cattcactcc
    11281 acatccggca ccgggtgttc catgcctgat gcgacgctgg tgtcttatct ggcctaaccc
    11341 attgaaatat ccacgcacct cactgcgcta ttctgggttg aactgcggat tcgccaacat
    11401 aaagccgcca tccactatca acgactgccc tgtcgtgtaa tttgcgccct ccgaacaaag
    11461 ccacaccacc aggctggcaa tctcatgcgt tgcgccaaaa cgccgtagag gaatcgaagg
    11521 ctccgcgtcg ggcttcacgt cgctatcatc catgctgttc attggcgtgg ctatcgcccc
    11581 aggcgcgact gcgttcacca aaatcttatg cctgaccaac tccagcgcca tcgttttggt
    11641 taacccgccg agcgcatgtt tagcggctgt gtaggcgctg gcatccggca gcggcgtatg
    11701 ttcatgtacc gacgtaatgt tgatgatgcg accgccctgc ccttatttca ccatctgacg
    11761 agccgcaatt tgcgagcata aaaatgcacc atcaacatca acggtaaaaa tcttgcgcca
    11821 ctcatcaaaa gccatatcaa gaaacggcgc tttggtcatt gcacccgcat tattcaccag
    11881 cacatcaata cgcccaaatc gctggatgag tgtctccagc gcctgcgcac cttctggcag
    11941 attgccgaga tccagttgca cgatctccgc acgtacgccg tgggtaacca cctcacgcgc
    12001 ggtatctttt gccccttctt catctgagtg ccaggtaatg ccaatatcaa acccctgctg
    12061 cgccagtaat aacgcgcact ctttgccgat ccccgaatcg gaagcggtaa ttatcgcgac
    12121 ctatgccatc gagttctcca cttaacgctg aataaacgtt aagtatagaa ggcgcatatc
    12181 atcagcgttt gtaacccccg cccagcgcac cggtgagtcg aatggaggca tccagccact
    12241 gcccttgcaa taacaggcca ttggcccgct cacgcagcgc cgggattctg gcttcgctga
    12301 cgcgggaacc agcaatgatg cccgcgttaa agcgggcctg cgcaagacct accacacgca
    12361 agacatcgcg ctcaatttgc gcctgatgct ggtttttctc cgccagtgtc tgaacctgac
    12421 tggctgcccg cgccacgtca ttcactgctt caaccaccgc tttgttgtag ctggcgatag
    12481 acaagttgct ttcggctttt gcgatatcga gattggcgtt aagacgacca ctatcgaaaa
    12541 taggtagcgt caggcctgcc gtaacgccca tttgctgcgc ggaatgacgg aacagatcgc
    12601 ttaagtgcaa cgcatcctgt tgcaggaagg ccatcaggtt gatgtcagga taaaatgccg
    12661 cttttgccgc atcaatagtg cttaacgatg actcaacgta ccagtgcgcc gcctgcaaat
    12721 ctgcccgccg ggccagtaag gagtacccca gttcatcagg aagctggctt gccactttcg
    12781 gcaacgcgac cggtttaagc ttcaatgact tagtctggtg atttgtgagt gcatttaacc
    12841 gtgcctcaat aattttcatt ttccccacga catcgttgag ctgctgccgg gttttgctgg
    12901 cattaatatc ggtttccata ccgtcaactg aagaagtaat cccgttctga tatagctggc
    12961 gatcggtcgc gataatggtg ttctgctctt tttctatttg ctgcaagacc gtgtttaacg
    13021 ccgcctgggt ttgccactcc cagtacaggc gggctacgct gccagccagc aattggcggg
    13081 tttgctcgcg ttccgccgcc cgtgctttaa ccgtacccag gcgggcagta acctccgccc
    13141 gattctttcc ccagatatcg agatgccagc ccgccgttaa gccaaaagtt ccgttggtgt
    13201 accacggacc ggtcgtacct gcggccggat cgttcagagc aaacggcccc attagccctt
    13261 ctgccgacat tttttgccgc tccatatccg ccgaaaagtc gatctgcgga ccatcctgag
    13321 tggcaactgc cttcgcctgg gcttcagcta gctgaatgcg ctgttcagcc acctgcatat
    13381 ccggtgcgtt ctgtagtgca ttgttaatta atgaagtgag ctgattatcg tgatactcca
    13441 gccaccattg gctgtctggc caaccatttt tcagcgccgt gggtaatgcg gtgtcaactt
    13501 gtgcagcggg cgtttgctgg cttaacgcct ggcgggtttc atgcataggc gcacacccgg
    13561 ccagcatcag taacagcgga aaacaggcga tggctggata aaaggaatca cgattcatgg
    13621 gggaataatc aggtaagaaa cggtgcgcgg agattaccgt gtgttgcgat atatttttta
    13681 gtttcgcgtg gcaatacatc agtagcaata aaacgacata cccagaaaaa tatagactaa
    13741 gtaaatgata tcttccgatt tatcttaatc gtttatggat aacggcaaag ggcttcgttt
    13801 tttcctatac ttattcagca ctcacaaata aaggaacgcc aatgaaaatt atactctggg
    13861 ctgtattgat tattttcctg attgggctac tggtggtgac tggcgtattt aagatgatat
    13921 tttaaaatta attaatgtca tcagatccga aaataacgag aatatttcag tctctcatcc
    13981 tgttgcgctc ctgtcatgtg cattgcttca tataatcact ggcgcaagga gcgcgcaggg
    14041 ggcgaccaat cgccgccgcc ccctgccccc ccgggctctg gcgaacaaaa tcgccgctgc
    14101 gcggtgccct cggcttatcc cttacggcta ccgggtcggg cgcgaggtaa catccctgta
    14161 aaacgcgccc tcagcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggcg
    14221 attttgacgc caccaaacaa ccgtgcggcc tattgataaa gagctaacac attgtcaaaa
    14281 acatcactat ggtttttaga gtttctcgat atcaattgcc tgaatagccc ttgcaatatc
    14341 aggggagtta ttcaacaccc ggg
//
LOCUS       DADMFL010000097        14307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.098, whole genome shotgun sequence.
ACCESSION   DADMFL010000097 DADMFL010000000
VERSION     DADMFL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.098"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(117..407)
                     /locus_tag="JNZ27_003024"
     CDS             complement(117..407)
                     /locus_tag="JNZ27_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665272.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1007..1082)
                     /locus_tag="JNZ27_003025"
     tRNA            complement(1007..1082)
                     /locus_tag="JNZ27_003025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1047..1049),aa:Phe,seq:gaa)
     gene            complement(1188..1895)
                     /locus_tag="JNZ27_003026"
     CDS             complement(1188..1895)
                     /locus_tag="JNZ27_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY8665273.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2293..4428
                     /locus_tag="JNZ27_003027"
     CDS             2293..4428
                     /locus_tag="JNZ27_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8665274.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4477..5733)
                     /locus_tag="JNZ27_003028"
     CDS             complement(4477..5733)
                     /locus_tag="JNZ27_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY8665275.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5935..7014)
                     /gene="mltC"
                     /locus_tag="JNZ27_003029"
     CDS             complement(5935..7014)
                     /gene="mltC"
                     /locus_tag="JNZ27_003029"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY8665276.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7079..7354)
                     /locus_tag="JNZ27_003030"
     CDS             complement(7079..7354)
                     /locus_tag="JNZ27_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY8665277.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7382..8434)
                     /gene="mutY"
                     /locus_tag="JNZ27_003031"
     CDS             complement(7382..8434)
                     /gene="mutY"
                     /locus_tag="JNZ27_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY8665278.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8595..9314
                     /gene="trmB"
                     /locus_tag="JNZ27_003032"
     CDS             8595..9314
                     /gene="trmB"
                     /locus_tag="JNZ27_003032"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY8665279.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSENMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9314..9640
                     /locus_tag="JNZ27_003033"
     CDS             9314..9640
                     /locus_tag="JNZ27_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY8665280.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            9824..10543
                     /locus_tag="JNZ27_003034"
     CDS             9824..10543
                     /locus_tag="JNZ27_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY8665281.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10719..11765
                     /gene="ansB"
                     /locus_tag="JNZ27_003035"
     CDS             10719..11765
                     /gene="ansB"
                     /locus_tag="JNZ27_003035"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY8665282.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11882..12889
                     /locus_tag="JNZ27_003036"
     CDS             11882..12889
                     /locus_tag="JNZ27_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY8665283.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12945..14246)
                     /locus_tag="JNZ27_003037"
     CDS             complement(12945..14246)
                     /locus_tag="JNZ27_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY8665284.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3743 a         3431 c         3696 g         3437 t
ORIGIN      
        1 gcatgggtat tttaattatt ttgatgttga tctaacgcac tcaaggacca ttttattacc
       61 gattatccat tctatttaac tgttgcgata taaatgtttg ttttaggtgc tttaattcag
      121 tacaagtcat caaaccgtac cagaccctga ctgtcagtga caccgacagg tgggggcggc
      181 ggtgtggggt caaactgagt gtgtctgcct ctggcccaca gagccagctc tgccgccatt
      241 gcccgtggat gatactccag ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc
      301 acgaactgca gcgccagcca gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc
      361 attttgtgac tcaggcaggt gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac
      421 ccttttaatt caatttgtta aattgctttc caccttttgc ggggcgcgac ttaaccaaaa
      481 tattcactgt tatcataata tgcaatgggc tggaggttgc aggtcattta gggattttct
      541 gtaagccccg ttttgcagaa cgttctatct ccgattgaga tcctgtctct cggtattacc
      601 ggtagtctgg ccataatccc ctatggctca tattgatata gtaggcataa cacccaaaag
      661 ttgactaaag ctaaatcaat cagagtgaaa aatgttcggt ttgatgtagg gggggactga
      721 ggaggggcca acgcaggtgg tatgcgtttt acttcagacc ggacaaagac cggatgtatc
      781 cgtgcagtaa tgaatccgtt agccaggtgc tcatgtggaa atagtgtcta ttggggattt
      841 gaagtttgac agaaaaggcc acagtgacca gaagggggct acttagaaga aatgttcagg
      901 ctggatggaa gaggtaaagc agtgacatca gcattcaaag tgaccggata tctaccagaa
      961 ccccagaaag caaaaaacca gcccgtaggc tggttcttct aaatagtggt gcccggactc
     1021 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     1081 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     1141 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     1201 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     1261 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     1321 gcgctgaagt ctgccatcat cgacggtgtg gtcagcggta atatcagcgc ggcagcccac
     1381 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     1441 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     1501 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     1561 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     1621 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     1681 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     1741 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     1801 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     1861 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     1921 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctag
     1981 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     2041 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     2101 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     2161 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     2221 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     2281 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     2341 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     2401 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     2461 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     2521 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     2581 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     2641 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     2701 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     2761 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     2821 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     2881 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     2941 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     3001 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     3061 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     3121 ctggcgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     3181 gataccattg ggcatctctg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     3241 cagtttatcc cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     3301 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     3361 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     3421 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     3481 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     3541 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     3601 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     3661 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     3721 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     3781 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     3841 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     3901 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     3961 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     4021 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     4081 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     4141 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     4201 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     4261 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     4321 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     4381 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     4441 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     4501 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     4561 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     4621 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     4681 catcaggaac gtcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     4741 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     4801 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     4861 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     4921 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     4981 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     5041 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     5101 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     5161 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     5221 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     5281 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     5341 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     5401 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
     5461 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
     5521 tacccatttc gcacttaacc atttgtcggc cacaatcccc agtagcgcag gcataaatac
     5581 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
     5641 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
     5701 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgattcgga
     5761 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
     5821 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
     5881 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
     5941 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
     6001 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
     6061 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
     6121 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
     6181 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
     6241 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
     6301 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
     6361 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
     6421 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
     6481 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
     6541 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
     6601 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
     6661 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
     6721 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
     6781 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
     6841 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
     6901 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
     6961 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
     7021 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
     7081 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
     7141 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
     7201 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
     7261 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
     7321 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
     7381 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
     7441 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
     7501 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
     7561 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
     7621 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
     7681 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
     7741 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
     7801 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
     7861 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
     7921 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
     7981 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
     8041 tgcccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg
     8101 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
     8161 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
     8221 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
     8281 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
     8341 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
     8401 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
     8461 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
     8521 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
     8581 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
     8641 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
     8701 atgcgctgga aaattactgg ccggtgatgg gcgttgagtt cagtgaaaat atgctggatt
     8761 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
     8821 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
     8881 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
     8941 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
     9001 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
     9061 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
     9121 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
     9181 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
     9241 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
     9301 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
     9361 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
     9421 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
     9481 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
     9541 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
     9601 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
     9661 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
     9721 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
     9781 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
     9841 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
     9901 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
     9961 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    10021 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    10081 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    10141 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    10201 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    10261 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    10321 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    10381 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    10441 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    10501 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    10561 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    10621 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat acaaagctaa
    10681 gggataatgc gtagcgttca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    10741 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    10801 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    10861 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    10921 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    10981 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    11041 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    11101 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    11161 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    11221 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    11281 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    11341 agattgacta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    11401 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    11461 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    11521 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    11581 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    11641 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    11701 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    11761 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    11821 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    11881 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    11941 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    12001 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    12061 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    12121 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    12181 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    12241 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    12301 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    12361 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    12421 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    12481 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    12541 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    12601 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    12661 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    12721 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    12781 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    12841 ctggaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    12901 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    12961 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    13021 aatgcagcat aaaatggcag caatggtttg attaaatgtt gaatcttcac acccgagata
    13081 gaacaaccaa caaatagcgc actacccacc ggcagtgtta acagcccaat acaaaggttg
    13141 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    13201 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    13261 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    13321 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    13381 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    13441 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    13501 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    13561 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    13621 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    13681 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    13741 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    13801 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    13861 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    13921 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    13981 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    14041 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    14101 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    14161 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    14221 agtgtaatcg cgatataact atccattaac tggcctcctg cgcaggttgt ttcagttgtt
    14281 gaatgctttg attaataaaa cacaagg
//
LOCUS       DADMFL010000098        13995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.099, whole genome shotgun sequence.
ACCESSION   DADMFL010000098 DADMFL010000000
VERSION     DADMFL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.099"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..170
                     /locus_tag="JNZ27_003038"
     CDS             <1..170
                     /locus_tag="JNZ27_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8665285.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
     gene            275..1627
                     /locus_tag="JNZ27_003039"
     CDS             275..1627
                     /locus_tag="JNZ27_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8665286.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1640..2398
                     /gene="chbG"
                     /locus_tag="JNZ27_003040"
     CDS             1640..2398
                     /gene="chbG"
                     /locus_tag="JNZ27_003040"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY8665287.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JNZ27_003041"
     CDS             complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JNZ27_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY8665288.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4889..5152
                     /gene="cedA"
                     /locus_tag="JNZ27_003042"
     CDS             4889..5152
                     /gene="cedA"
                     /locus_tag="JNZ27_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY8665289.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5429..6245
                     /locus_tag="JNZ27_003043"
                     /pseudo
     CDS             5429..6245
                     /locus_tag="JNZ27_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6249..7639)
                     /locus_tag="JNZ27_003044"
                     /pseudo
     CDS             complement(6249..7639)
                     /locus_tag="JNZ27_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(7772..8362)
                     /locus_tag="JNZ27_003045"
     CDS             complement(7772..8362)
                     /locus_tag="JNZ27_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8665290.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="JNZ27_003046"
                     /pseudo
     CDS             complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="JNZ27_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9339..9875
                     /locus_tag="JNZ27_003047"
     CDS             9339..9875
                     /locus_tag="JNZ27_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665291.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9916..10776)
                     /locus_tag="JNZ27_003048"
     CDS             complement(9916..10776)
                     /locus_tag="JNZ27_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY8665292.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="JNZ27_003049"
     CDS             complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="JNZ27_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY8665293.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="JNZ27_003050"
     CDS             complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="JNZ27_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY8665294.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSTALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12488..13242
                     /locus_tag="JNZ27_003051"
                     /pseudo
     CDS             12488..13242
                     /locus_tag="JNZ27_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            complement(13295..13402)
                     /locus_tag="JNZ27_003052"
     CDS             complement(13295..13402)
                     /locus_tag="JNZ27_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665295.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13574..>13995)
                     /locus_tag="JNZ27_003053"
     CDS             complement(13574..>13995)
                     /locus_tag="JNZ27_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665296.1"
                     /translation="DGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLK
                     GATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAV
                     HIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3613 a         3218 c         3451 g         3713 t
ORIGIN      
        1 tccgtattaa ttttgccaaa aaacaactgg aaatgaccaa ctattctgtg acggatattg
       61 cgtttgaggc cggttatagt agcccgagtt tgtttattaa aacgtttaag aaattaacgt
      121 cctttacgcc taagagatat cgtaagaaat tgacggaatt taatcagtaa gtcgttatac
      181 ctgacaattc acatatctgt cctgttgctg gattatttat gtccgggggg gcagatatgc
      241 cagatatcag tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac
      301 tattggtggc gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca
      361 cgaattgccg gtcagcgaat tatggctggt ggatgtcgag ggtggcaaag ctaaactgga
      421 tattattttc gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta
      481 taaaacgctg gattgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg
      541 cgttggccaa ttaccggcgc gtgaactgga tgaacgtatt ccattaagtc atggttatct
      601 tggtcaggaa accaacggcg cgggcggttt gtttaaaggt ctgcgtacca ttccggtgat
      661 ttttgacatc gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga ttaacttcac
      721 taacccggcg ggaatggtta ctgaagccgt ttatcgtcat accggattta aacgctttat
      781 cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cacgatgttc tgatgctgaa
      841 agacagcgat gatttatcta tcgatttgtt tggcctcaac catatggtgt tcattaagaa
      901 tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg catccgggca
      961 gttaaaagca tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg
     1021 ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct
     1081 ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag tacagaaagt
     1141 cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc cgaaagaact
     1201 ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca acgctatcta
     1261 caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata ttgataatat
     1321 tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg cgacgccaca
     1381 tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca ttaaaggctt
     1441 cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt tactggcgct
     1501 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
     1561 tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc
     1621 acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg attttggctt
     1681 aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacatcgac
     1741 gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc gtgatgaacc
     1801 gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga cagctatgcc
     1861 ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc
     1921 tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt tcattgagct
     1981 atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat
     2041 tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca tcgaccgtca
     2101 gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg gattcagtag
     2161 cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg atgacgcaag
     2221 ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg ataatactat
     2281 ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga catcagcgtc
     2341 gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg atgtgtaagt
     2401 aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc cggaattttg
     2461 tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa cagttcatcc
     2521 ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg gtcagctacc
     2581 ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt aaggtgtttg
     2641 taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc gatattgccg
     2701 caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt agcggctata
     2761 ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag cagtttggca
     2821 tgaacgcctt tggccttcag ggccttgaga atggcaagaa ggtctgccga tctcacttca
     2881 tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat ggcgtacaaa
     2941 cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat attcagctgg
     3001 tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt gagatcaata
     3061 tgcgccagct ggtcaacaac gcgctcacga atatacggac gaacgacttt gcttaactca
     3121 aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag ccagaacaga
     3181 cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt attgccttcc
     3241 acgcgctcct ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt ttcgcgcggc
     3301 cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat ccccatacga
     3361 tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat cggaatctca
     3421 tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa caaacgtccc
     3481 tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg atggaatgct
     3541 gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat tttgccgaca
     3601 cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag atcgaagtcg
     3661 aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc cggaaaatcg
     3721 cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg tccggtgagt
     3781 ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca gtggaaacgt
     3841 acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc gaagccttcc
     3901 atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac gttgtgcaga
     3961 gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg cccttgtgga
     4021 attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt atgcgcatcc
     4081 tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc ttcagtatag
     4141 aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc gccaccctga
     4201 acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag gaaatccgct
     4261 ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc tgatccgcgt
     4321 gcatgaacaa tacgttccgg aatgcgctca tggtcaaagt gggtgatttt ctcgcgcaga
     4381 ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt ttgatcatcg
     4441 gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt gcgtacgtct
     4501 tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc aggggcggta
     4561 ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc ctcaggtgcc
     4621 agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg gtgctgatgt
     4681 gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta ctgaaagggc
     4741 cgcttttttg gtcaaaaaac tctctaacta tagaccacca gccagatccc gcttcggaga
     4801 ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg gctaaatcgg
     4861 ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa tgaagaaacc
     4921 gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac ccgcgccgcc
     4981 agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg gcaaatatgt
     5041 tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg tgcctgaatc
     5101 tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt aaggattaca
     5161 tacctcctgg ttttgccggg agttacagga cctgaaattc tgcatttcgt tgctacattc
     5221 attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa ataagtgatc
     5281 ttttttcttt aattgtttat ttattttaaa atggttaagt tttgctaatc atgatgagtt
     5341 atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat ggcttagatt
     5401 taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta caaacttaat
     5461 acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt ttaaagaaag
     5521 gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat gtgactgcga
     5581 tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca ttaactatga
     5641 tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg acaatgttaa
     5701 atatagagtg ataattaata atctttatgg cagtgagttt cggataagag atattaatga
     5761 ggattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga cgaatttgat
     5821 caatataaaa aataggttgg ttattcatta tttaaggact atatactatg aaaaaaatta
     5881 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
     5941 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
     6001 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
     6061 gtctcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
     6121 ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg
     6181 ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga ccgttgccag
     6241 cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg
     6301 gtttgcttca gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc
     6361 gccgtacggc ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact
     6421 ggcaggccca tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg
     6481 gcaacgcctg cggaactaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc
     6541 agcgggttaa tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg
     6601 gcgcagccgt tctgaccaat ggttgcaccg aaagaggcgg cgaaactggc gatggattca
     6661 ggaacgccca gacgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa
     6721 cggctggtaa aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc
     6781 acgccattaa tgcccagcag aatgccatgc acagcaaaca taatcagcag accgaggtag
     6841 gacgcgacaa cgaaacttcc cagtttgatg atgtcttgca agttagaacc tgcaaccact
     6901 ttggtcatta gagccagaac gccgtaaggg gtcaactgca tgacaaggcg aaccagtttc
     6961 atcacccagc tttgtagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca
     7021 tccttcagca gtttcaatgc agctacgccg aggaatgcgg caaaaattac cacgctgata
     7081 attgacgtcg gattggctcc ggtaagatcg gcaaacgggt ttttcgggat aaaggacaag
     7141 accagctgcg gaacgctcag atcagagact ttaccaacat agttactttc aatggcgttc
     7201 agacgtgcag tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggtgg
     7261 tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca
     7321 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     7381 aggcgaagac taacggcata acgatcattt gcagcagttg aacattgccg ttaccaacga
     7441 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     7501 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     7561 actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     7621 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     7681 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     7741 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     7801 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct
     7861 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     7921 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc
     7981 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca
     8041 gcaccattcc ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa
     8101 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     8161 cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat
     8221 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     8281 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     8341 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     8401 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     8461 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg
     8521 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     8581 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc
     8641 aggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg
     8701 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag
     8761 gcagtttttc aaccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     8821 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     8881 tgcataacgc aacagcttcg cgcacggctg gtaataatgg acgtgtctct tcaaccagtg
     8941 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     9001 gccgggcgta caaagatcga ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc
     9061 gttacgacgg gagatatcta cccccaggct tgccatcaca tccagttcgg ctcgatccca
     9121 taaaggttct gagtcgataa gtaatccatc catatcaaaa attgcagcaa gaatctgacg
     9181 cggggttgac attacaacct ctcctttaga gcattatgtg taaggtatta agccgtgctt
     9241 atgattaagc gcctatcata tcgcctttaa agaatgggcg aaaacagata tctacaggta
     9301 aactaagtga aacgacgcgt cttaatcaag gagaactcat gacgtatcaa caagctggac
     9361 gcattgctgt tttgaaaagg attttgggtt gggtgatttt tatacctgca ttgatctcta
     9421 cattgatttc tttgctgaaa tttatgaata cccggcagga aaaccaggaa ggcattaatg
     9481 cggtcatgct cgactttgct catgtcatga tcgatatgat gcaggcgaat acgccttttt
     9541 tgaatctgtt ctggtataac tccccgaccc ctaattttaa tggtggtgtg aacgttatgt
     9601 tctgggtgat tttcatcctg atttttgtcg gactggcgtt gcaggattcc ggtgcccgga
     9661 tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag
     9721 ccaaagggga agaggggcta acgcgtgaac aaattgagtc ccgtattgtt gttccgcacc
     9781 atactatttt cctgcagttt ttctcactat atatcctgcc ggttatttgt attgctgcag
     9841 gttatgtgtt cttttctctg cttgggttta tttaatcaaa aggccgcacg gagcggccat
     9901 tatcctcaac ccatatcatg ctgctaataa tctatccaat gactgctgag caataaccaa
     9961 atgctgaccg ccaaataatc ttgcacgatt taacagcgtg tagagttggt aaaccggttg
    10021 acgttcaagg aaatcggcag gtagcgggga tactgactga tagccgtcat agatttgtgg
    10081 cggctgttca gtatgcagcg gtaacatcgc caggtcgcac tctcggtcac cccagtagca
    10141 ggcagggtcg aaaatgtacg ggccatccgg acccagcgca cagttgccgg accataaatc
    10201 gccgtgcaac agagaaggct gcggttgatg cgaggccaga cgctgctgaa tatgctcgac
    10261 gatggcgtcg atattgccga aagcgatccc tttctctgct gccagttcca actgccagcc
    10321 aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc caggtgttgg gctgtggagt
    10381 tgtggagagc gagttatcaa aatcgaggcc aaattgtggt tggtcactcc attgatgtaa
    10441 acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca tccagcggac gaggtgggag
    10501 ataatccatc accagaaaac tgtagtcacg gtcagcgcct actgcccaaa ccttaggcac
    10561 ggtgacggtt ttactacgcg acagtaactc cagttggtcg gcttcggcgg taaaaccggg
    10621 aagcagttcc ctttcatcac atttgacgaa aaagtcatgt cctgcatagc gcaaatgcca
    10681 tgcggcatgg acttctccgc caggcagttc attacgcagt tcgatttcgc cttcacctaa
    10741 ctgctcgctc aaaagacgac tgattgcctg ccacatgatg tctctcccat gttgtctgcc
    10801 agatcataag gttagcgcaa gaatgcggtg taaaaacacg agctaacgca caccacagac
    10861 tgattattgc ccaaaaacca tttattttct gttgctattc cattcctccc aggtcgtgat
    10921 ggtgatttca ggatctttgc cggttgcact ttgggtaagc cccgaaacca gttctctaat
    10981 ttcttcttca ctttgggtac tgataagtcc aaaagtgtta gtccccagct catggatatt
    11041 tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc
    11101 gttaatttca gtcaatgtat cctcatgcaa catgacggtt atgacataac ggacaagatc
    11161 gccactggcc atagttcacc cctttgttat cacatgactt ttgagcatag tcggagaaac
    11221 gcgttgccga caggttggtg gtgattcccc caatgctggg ggaatgtttt tgttagcggg
    11281 aaaggtaagc gtaaattttt tgcgtatcgt catgggagca cagacgtgtt ccctgattga
    11341 gtgtggctgc actccccgca gctacgccaa aacggaccat ctcttcaaga gaggcatttt
    11401 ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc agcgccaacg gtactctggc
    11461 ttttcaccgg tggtggcacc acctgaatgc agttttcact atcaacaccc agcgctcctt
    11521 gtggaccaag ggaaacgaca acccgtttgg ccttgccgct attaacgatt tcctgcgcgg
    11581 ctttgcggac atcgtctggc tgggtgagtt cgcgactcac cagcgcactg agttcttttt
    11641 ggttaggctt aaccaactcg atgttaccaa tcgccagtgc tgtacttaac gcttcgccag
    11701 aactgtcgat gatgcagcgg atcccttggt tttgcgcagc ggaaatcagt tgggttaatt
    11761 tttccagctt cacacctggc ggcaggcttc cgcttatgac caggatggcc ccggattcaa
    11821 tttccagaac ttgctcttca agctggcgaa actcatcttc atttaatgcc gcgcctggca
    11881 taacaaaacg atactgttca ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc
    11941 agtctttggc ttctacagta gcgacgggga cattttcatc cgccaacagt gaaaccaggt
    12001 gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc actgcctcca agatgggcaa
    12061 tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa caccggtgcg gtacagcgca
    12121 gttttccttc gggataaatt tgcggggtaa ttgttgcgct atcgagagag ggcgcaagtg
    12181 tcaacgtata gatacgtacc atcatttcct cctataggct gatttcagtc tggcaccgaa
    12241 tcagcggaaa gtgaagaaat taacaatatg atttattaga gctttattta aaattttgca
    12301 gataaatata tataaataaa aatctgtttt cgccacatgc ttattcaaaa aatgaaataa
    12361 gaatacattg ctaagttatt cgcgcctttt tataattcgt tacctttcca gggacgctaa
    12421 tctttgatcc ttaagaaagt ttccgggacc gtgatggtct cttttttgtt gtacggactc
    12481 ttgataaatg aagcttttga agacagttcc tgccatcgtt atgttggcgg ggggcatgtt
    12541 tgcgtcactg aatgcagctg ccgatgattc cgtttttact gtcatggacg atcctgccag
    12601 cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat cttgcacaat caggcaatac
    12661 gaaaagctcc tcacttacgg ctgacaccac catgacctgg tatggacaaa ccactgcctg
    12721 gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat gagcgttctt cagaaaaata
    12781 tgcggcgggc gtagccgctt taatttaact gattatgact atttatttgg tcaggcaagc
    12841 tggctgacag accgttataa cggctatcga gagcgcgatg tgttgaccgc gggttatggt
    12901 cgtcagtttt tgaatggccc ggtacacagt ttccgctttg aattcggtcc aggtgtgcgt
    12961 tacgacaaat ataccgacaa tgccagtgaa acccagccgc tgggctatgc ctccggggct
    13021 tatgcatggc agttgactga caacgctaaa tttacgcagg gtgtttcggt ctttggcgcg
    13081 gaagacacaa cgctgaactc cgaaagcgca ttaaatgttg ctattaatga acattttgga
    13141 ctgaaggtgg cttacaacgt cacatggaac tctgaacctc cagagtctgc accggaacat
    13201 accgaccgtc ggacaacctt atcgctgggc tacagcatgt aaaattatct gccggaccgt
    13261 gttatgcggt ctggctgtct tcgtttgtca ctgctcagcc ccaaatcatt gctgcccaac
    13321 gtagcaacaa catagcgcca cagattgcca gtagcaccac caccatcttc cagtgttttt
    13381 tatcaaagcg tttagtcggc ataccagtgt cctgcatatc tcttgtgcgt gataacggag
    13441 tgtaacaaaa gggacaggca tcaagggatt ttttgttatt acagccgtcg ggtggcgttg
    13501 tgcacatggt cactgtttct gcgttttatg aaaaaaataa aattatgtat cgatatatat
    13561 cgtaaatagc tggttataaa tacgtcttta tttcatcagc actaaaactc aggctttggc
    13621 tgatgacatt tatagcagca taatatgttt ctacagtttt ataatgctta tgatgaatgg
    13681 ctatatgaac agctgacatg ttgtcatgat ttttagcggc caatagtgta aataactgat
    13741 gttgactaaa agaatgtttt tttgcaaaag cacccagcgt cgatatataa gataacacga
    13801 cgtcttcatt acccttgctc atggcgatgt ataatgcagg ggttccctgt gctgtagctc
    13861 cttttaataa atccatgatg ttagctttgc tcaacttgta tttaaaggcg atgccattaa
    13921 ttttagagag gaaccgtgtg acacacaaag ggtgattatt ttccattgcg gcgtataatc
    13981 ctggctcacc atcat
//
LOCUS       DADMFL010000099        13956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.100, whole genome shotgun sequence.
ACCESSION   DADMFL010000099 DADMFL010000000
VERSION     DADMFL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.100"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="JNZ27_003054"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="JNZ27_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY8665297.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="JNZ27_003055"
     CDS             complement(1604..1777)
                     /locus_tag="JNZ27_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY8665298.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="JNZ27_003056"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="JNZ27_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY8665299.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="JNZ27_003057"
     CDS             3415..3744
                     /locus_tag="JNZ27_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY8665300.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JNZ27_003058"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JNZ27_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY8665301.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JNZ27_003059"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JNZ27_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY8665302.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JNZ27_003060"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JNZ27_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY8665303.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JNZ27_003061"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JNZ27_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY8665304.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JNZ27_003062"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JNZ27_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY8665305.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JNZ27_003063"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JNZ27_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY8665306.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="JNZ27_003064"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="JNZ27_003064"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY8665307.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGYKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3453 c         3247 g         3598 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacaactgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcta caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DADMFL010000100        13954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.101, whole genome shotgun sequence.
ACCESSION   DADMFL010000100 DADMFL010000000
VERSION     DADMFL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.101"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..493)
                     /locus_tag="JNZ27_003065"
     CDS             complement(<1..493)
                     /locus_tag="JNZ27_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665308.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JNZ27_003066"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JNZ27_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY8665309.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JNZ27_003067"
     CDS             complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="JNZ27_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY8665310.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2440..3306
                     /gene="folD"
                     /locus_tag="JNZ27_003068"
     CDS             2440..3306
                     /gene="folD"
                     /locus_tag="JNZ27_003068"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY8665311.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3308..3520
                     /gene="ybcJ"
                     /locus_tag="JNZ27_003069"
     CDS             3308..3520
                     /gene="ybcJ"
                     /locus_tag="JNZ27_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY8665312.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3628..4149
                     /locus_tag="JNZ27_003070"
     CDS             3628..4149
                     /locus_tag="JNZ27_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8665313.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JNZ27_003071"
     CDS             complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="JNZ27_003071"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY8665314.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5744..6238
                     /gene="ppiB"
                     /locus_tag="JNZ27_003072"
     CDS             5744..6238
                     /gene="ppiB"
                     /locus_tag="JNZ27_003072"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY8665315.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6241..6963
                     /gene="lpxH"
                     /locus_tag="JNZ27_003073"
     CDS             6241..6963
                     /gene="lpxH"
                     /locus_tag="JNZ27_003073"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY8665316.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7081..7590
                     /gene="purE"
                     /locus_tag="JNZ27_003074"
     CDS             7081..7590
                     /gene="purE"
                     /locus_tag="JNZ27_003074"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY8665317.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7587..8654
                     /gene="purK"
                     /locus_tag="JNZ27_003075"
     CDS             7587..8654
                     /gene="purK"
                     /locus_tag="JNZ27_003075"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY8665318.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIANRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8791..9684)
                     /locus_tag="JNZ27_003076"
     CDS             complement(8791..9684)
                     /locus_tag="JNZ27_003076"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY8665319.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9681..10328)
                     /locus_tag="JNZ27_003077"
                     /pseudo
     CDS             complement(9681..10328)
                     /locus_tag="JNZ27_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10331..11554
                     /gene="allC"
                     /locus_tag="JNZ27_003078"
     CDS             10331..11554
                     /gene="allC"
                     /locus_tag="JNZ27_003078"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY8665320.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLA"
     gene            11574..12359
                     /gene="allE"
                     /locus_tag="JNZ27_003079"
     CDS             11574..12359
                     /gene="allE"
                     /locus_tag="JNZ27_003079"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY8665321.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12587..13732)
                     /gene="glxK"
                     /locus_tag="JNZ27_003080"
     CDS             complement(12587..13732)
                     /gene="glxK"
                     /locus_tag="JNZ27_003080"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY8665322.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGIGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13754..>13954)
                     /locus_tag="JNZ27_003081"
     CDS             complement(13754..>13954)
                     /locus_tag="JNZ27_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY8665323.1"
                     /translation="QQITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSN
                     GLLVGILLAVLMDNLIPWERIE"
BASE COUNT         3391 a         3678 c         3495 g         3390 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga taaacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa gattgctttg
     1261 gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac aaagtgaacg
     1321 tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta ctttgttata
     1381 agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca taataaaagt
     1441 cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga
     1501 aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc tattcccatc
     1561 aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg ttgctgttgt
     1621 tgtatttgta ctggatgcaa tggccaataa attatcattg attgcatcag cctgaccaga
     1681 aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact gaatggtgaa
     1741 tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat attgaccgag
     1801 tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca ccagttcgcc
     1861 ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag cggcgaatag
     1921 tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt caatctcatt
     1981 tatcgactcc acatccgtat ataaccgatt actttattta agacactgat agtagtaaaa
     2041 ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc taaaaaatag
     2101 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
     2161 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
     2221 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg tacgaaaaat
     2281 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
     2341 cacgcgataa atctgaaaca aaacctgaca gcgcgccccg cttctgacaa aatagacgca
     2401 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
     2461 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggctcgtatt
     2521 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
     2581 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
     2641 tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat
     2701 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
     2761 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac
     2821 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
     2881 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
     2941 tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg caccaccacc
     3001 gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctgttgatc
     3061 gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaagaagg cgcaattgtg
     3121 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     3181 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     3241 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt
     3301 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     3361 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     3421 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     3481 agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     3541 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     3601 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc
     3661 ctttgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     3721 atcatcctag cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg cgttgcttac
     3781 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     3841 ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg ctgctggctg
     3901 tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac tggcggtaag
     3961 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     4021 aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca ggtgattatt
     4081 tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc
     4141 agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt acgacgccag
     4201 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgtgcc
     4261 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     4321 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     4381 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     4441 gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagcacg
     4501 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     4561 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagaggcgc
     4621 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag
     4681 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     4741 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccattccg
     4801 gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg cgcgatttcg
     4861 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     4921 agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc cgcgccccac
     4981 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     5041 ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc gagatcctga
     5101 cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     5161 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     5221 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     5281 tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     5341 cgtttgataa ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     5401 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccaata
     5461 tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata
     5521 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     5581 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     5641 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     5701 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     5761 caatcacggc gatattgtca tcaaaacttt tgacaataaa gcacctgaaa cagttaaaaa
     5821 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     5881 cggctttatg attcagggcg gcggttttga accgggcatg aaacaaaaag ccaccaaaga
     5941 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     6001 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     6061 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     6121 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtaacggtat
     6181 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     6241 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     6301 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     6361 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     6421 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     6481 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     6541 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     6601 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     6661 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc
     6721 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgctgtggtc
     6781 agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg ggcataccca tcgcccggcg
     6841 gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg tgcctggcat
     6901 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     6961 taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     7021 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     7081 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     7141 gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca ccacgttgaa
     7201 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     7261 aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt
     7321 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     7381 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     7441 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     7501 gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga cgaagtgctg
     7561 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     7621 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     7681 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     7741 gatggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     7801 atgtgttccc gattattgct aaccgtctga ctcagaaaca gcttttcgat aagctccacc
     7861 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     7921 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     7981 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt gaatgtattg
     8041 tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc ggctttgatg
     8101 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     8161 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     8221 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     8281 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     8341 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     8401 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgtgaatt
     8461 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     8521 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     8581 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     8641 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     8701 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8761 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     8821 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     8881 gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     8941 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9001 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9061 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     9121 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9181 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc gcaaatattt
     9241 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9301 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     9361 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9421 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     9481 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     9541 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     9601 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     9661 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     9721 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     9781 gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga ggcgaaatat
     9841 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     9901 tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata ataccaggcc
     9961 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10021 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10081 tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc gaaaagtccg
    10141 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga aggcggcgta
    10201 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
    10261 tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10321 gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct ggctttcctc
    10381 ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg aatggctgga
    10441 aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac gtttcgatga
    10501 agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag tggttctgag
    10561 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    10621 ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt tacgtacggt
    10681 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    10741 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    10801 aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgcctaacg ccccactaac
    10861 tccgcgtcag gatataaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    10921 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gctatacggt
    10981 gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    11041 ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga
    11101 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    11161 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    11221 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    11281 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    11341 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    11401 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc ccagcatcaa
    11461 cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg aaggggtcaa
    11521 aacgttggca ctcatgcttt atcaacttgc ctagcagaaa taaggagtca taaatgggat
    11581 atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg attgttaaac
    11641 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    11701 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    11761 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    11821 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    11881 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    11941 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    12001 caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat gaaggtatgg
    12061 atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg aacatgcata
    12121 tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    12181 gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg atcccggtga
    12241 aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat ggcgtagggc
    12301 gtggtgaagc gttcagctat atttactcga aagattgcaa ccgcgacgta gagatttaag
    12361 tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt tataccgtgg
    12421 ctttttttgt aacattgcct gatgcgcttc gcttatcagg cctacatgac ctctgcaata
    12481 tatagaattt gcgcgatttt caggaaggat atagtactca tgccgcatcc ggcatgagta
    12541 ctgcgctctt atacgaaata aatcgcatcc gctttaaagg caagagtcag tttttaattc
    12601 cctgacctat tttaatggcg caggcaatat ttcgcgcgct gttgaagaga ttggtttcac
    12661 cgctggcgag cacttcggct aaaggtgcca gacgaggcaa aatgctgaat agcgcgtcaa
    12721 tgccgtactg gtgcaccact tccacgccat cacccaatac accagcaatc ccaatcaccg
    12781 gtacattaaa ctgcttcgcc accgacgcaa cgcccagcgg cgctttaccg cctgccgttt
    12841 gcgagtcaat gcgcccttcc ccggtgatca ccagtgctgc cccctgcacc gcctgcgcaa
    12901 gattgaccgc attcaacaca atctcaatgc ccggtttaat atccgcattg agaaataccg
    12961 ccgccgcaat ccctataccg cccgcagcgc cgccgcccgc catctgacag acattaattt
    13021 cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc ctgttccagc tcttcaacca
    13081 tctccggcgt tgcccctttt tgcgggccaa ataccgccgc cgcgccgcgt gcccctacca
    13141 gcgggttatc aacgtcacat gccacttcaa tatggctgtt cgccagacgc ggatcgcatt
    13201 cctccatctc aatgcttgcc acgcgcgcta aattgccgcc gttagccgcc agcacctggc
    13261 cgtcggcatc aaggaaacgc acaccaagcg cctgcgccat gcccataccg ccgtcgaccg
    13321 tcgcactgcc gccaatgccg agaataatat ggcgaatgcc gttatccagc gcatggcgaa
    13381 ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt acgcttttca ggcgcgacca
    13441 gcatcaggcc acttgctgcc gccatctcaa tcaccgctgt tttcccgtct ccggtaaggc
    13501 cataaaaagc attcactttt tcgcccatcg gcccgcagac ttcaagcgtc acgatgttgc
    13561 cacccgtcgc ggcgaccatc gcatccaccg tcccttcgcc gccatccgct atcggcaaac
    13621 agatatagtg cgcatcggga aagagggtcg aaaacccggc tttaattgcc tgacaacatt
    13681 tttctgcact taagctctcc ttaaaagagt ctggcgcaat gacaatcttc attatcaatc
    13741 ctttttcaac aaattattcg atgcgttccc acggaataag attatccata agaacagcca
    13801 gtaaaattcc gaccagtaag ccgttactga gcagaggacg aagcgttaat ggaaggtctt
    13861 gcaaatacac aggcggtaat gccattaaaa atatgccgac aaataacggc aatgcgagtc
    13921 gataaatatt gcgctcggta aacgttattt gctg
//
LOCUS       DADMFL010000101        13896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.102, whole genome shotgun sequence.
ACCESSION   DADMFL010000101 DADMFL010000000
VERSION     DADMFL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.102"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNZ27_003082"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNZ27_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY8665324.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNZ27_003083"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNZ27_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY8665325.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNZ27_003084"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNZ27_003084"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY8665326.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKKVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNZ27_003085"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNZ27_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNZ27_003086"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNZ27_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY8665327.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNZ27_003087"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNZ27_003087"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY8665328.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /locus_tag="JNZ27_003088"
     CDS             complement(6140..7279)
                     /locus_tag="JNZ27_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY8665329.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7938..9521
                     /locus_tag="JNZ27_003089"
     CDS             7938..9521
                     /locus_tag="JNZ27_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8665330.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNZ27_003090"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNZ27_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY8665331.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNZ27_003091"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNZ27_003091"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY8665332.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNZ27_003092"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNZ27_003092"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY8665333.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..>13896
                     /locus_tag="JNZ27_003093"
     CDS             13480..>13896
                     /locus_tag="JNZ27_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8665334.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLG"
BASE COUNT         3225 a         3668 c         3512 g         3491 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cactttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgccgacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtacgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatcc gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgtttat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg ttgggg
//
LOCUS       DADMFL010000102        13857 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.103, whole genome shotgun sequence.
ACCESSION   DADMFL010000102 DADMFL010000000
VERSION     DADMFL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.103"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(84..440)
                     /locus_tag="JNZ27_003094"
                     /pseudo
     CDS             complement(84..440)
                     /locus_tag="JNZ27_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(576..683)
                     /gene="rprA"
                     /locus_tag="JNZ27_003095"
     ncRNA           complement(576..683)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNZ27_003095"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(869..1981)
                     /gene="ydiK"
                     /locus_tag="JNZ27_003096"
     CDS             complement(869..1981)
                     /gene="ydiK"
                     /locus_tag="JNZ27_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY8665335.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLFRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWCLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            2370..5426
                     /locus_tag="JNZ27_003097"
     CDS             2370..5426
                     /locus_tag="JNZ27_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8665336.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAMVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPTFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            5423..5833
                     /gene="menI"
                     /locus_tag="JNZ27_003098"
     CDS             5423..5833
                     /gene="menI"
                     /locus_tag="JNZ27_003098"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY8665337.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5933..6121
                     /locus_tag="JNZ27_003099"
     CDS             5933..6121
                     /locus_tag="JNZ27_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665338.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6669..7037
                     /gene="sufA"
                     /locus_tag="JNZ27_003100"
     CDS             6669..7037
                     /gene="sufA"
                     /locus_tag="JNZ27_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY8665339.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            7046..8533
                     /gene="sufB"
                     /locus_tag="JNZ27_003101"
     CDS             7046..8533
                     /gene="sufB"
                     /locus_tag="JNZ27_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY8665340.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            8543..9289
                     /gene="sufC"
                     /locus_tag="JNZ27_003102"
     CDS             8543..9289
                     /gene="sufC"
                     /locus_tag="JNZ27_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY8665341.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            9264..10535
                     /gene="sufD"
                     /locus_tag="JNZ27_003103"
     CDS             9264..10535
                     /gene="sufD"
                     /locus_tag="JNZ27_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY8665342.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            10532..11752
                     /gene="sufS"
                     /locus_tag="JNZ27_003104"
     CDS             10532..11752
                     /gene="sufS"
                     /locus_tag="JNZ27_003104"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY8665343.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11765..12181
                     /gene="sufE"
                     /locus_tag="JNZ27_003105"
     CDS             11765..12181
                     /gene="sufE"
                     /locus_tag="JNZ27_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY8665344.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            12330..13334
                     /gene="ldtE"
                     /locus_tag="JNZ27_003106"
     CDS             12330..13334
                     /gene="ldtE"
                     /locus_tag="JNZ27_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY8665345.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESEQNGEPEQGNMLRATQ
                     "
     gene            complement(13398..13634)
                     /locus_tag="JNZ27_003107"
     CDS             complement(13398..13634)
                     /locus_tag="JNZ27_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY8665346.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3521 a         3369 c         3690 g         3277 t
ORIGIN      
        1 aggtgcttta aaaagtgtag cgatgttgtg tcagtatcca ttcattattg ttgtaacaac
       61 gacaattcct ttctgtggaa aaattaacaa agccgctcca gatcgtgtgc atataattca
      121 gcggctaccg attgatagat cttccagtca ataaaattgc catcagggta gacttgttga
      181 aacgcggtta agtcggggaa aaaacgcatg gtgttgttgc cgatgcggtt atttattttc
      241 tctatgatgg catgaagatg tttttttcgt tactgacgca tagccaacag ttgctcgact
      301 acacaatcag gaatggcgca tttgccattt tcccactgac gccagctttc actgtcaccg
      361 gtctgggcaa tccaggtcgc gcattcatca atagtcatgg caaaaatatg gcggagtgcc
      421 tggagttcat aagcgttcat cgtgtaatgg ctgcatgaaa taaacaataa aatataaata
      481 acattatcag cctgctgacg gcttgaagag agtcacagta tcttgtgcaa cattattgga
      541 aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga
      601 tgggcaaaga ctacacacag caattcgttg tttcactcag gggatttcca tgcttataaa
      661 tcaatatgtt gatttataac cgtgagctaa tagtaggcat acggactttt tgcgtcgatc
      721 agattcgtct cattagttta aaaactgtaa agaatttgcg acaggaacta ccagctgaat
      781 ggcggtaagc aggaaaatat ggggaagagc aagcaaatat caattagcgg tgattaacaa
      841 tcaggcggca gagccgcctg atgagaaatt acttattcgg tttctcgatt tcgccaagtt
      901 cttcgagaat ttcttccggt tggtccgtcg gcggcgggac ttcttccacc cacgcggcaa
      961 acagacacca ggaaacggct aacagaaccg gaccaataaa cagaccgatc atcccgaaag
     1021 caatcaaacc accaataacg ccagagagga tcaggatcag cggtaaatcg gcacccatgc
     1081 gaattaacat tgggcggatg acgttatcca gcgtgccaac cacaccgctc cacactaaca
     1141 ataccgttcc ccaggtggta tcgccggtcc agtagagcca gataatcgcc ggaatcagta
     1201 ccggcaacgg gccaagctgg acaaggcagg agaggatcat taacaccgtt agcagcgttg
     1261 cataaggtac gccggatacg gcgaggccga taccgccaag cactgcctgt actaacgccg
     1321 tcaccaccac acccagcgcc accgcgcgga tagcctgtgc cgccagcagc acggcggcat
     1381 ctccgcgaac gcctgccaga cgggttgcaa aatggcgaat gccttgtgcc acctgttcac
     1441 cgcgccagta tagcagggca ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc
     1501 cgatatgcgc cgcctgccca acgaaccagg tggtggtggt gccaatataa gggcggactt
     1561 tcgccatgat cgccgtaccc cccatatcca gcaagttgtg ccagcctgca tacagcttcg
     1621 cgccaatcac cggaatggta ttaagccacg ccagatcggg taacgtcatg tcgccgctgg
     1681 aaatggcttt aattagcggg ccgctgccgt cgacgatact gttaaccagc aaagcaatag
     1741 ggatgataaa caccatcact aataacagcg tcatcaccag aacggcgaga gagcggcggc
     1801 caaacatgat cttttgcaaa cgaaacaata ccggccaggt ggcgataacc accgtaccgg
     1861 cccatgcaaa gccgagaata aagggttgaa caatccacag acatgccaca atcatgatgg
     1921 ctaaaaacag caccgaaagc agaatttgtg cgacatccct gggctgacga acatttacca
     1981 taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtggac acgcgaaact
     2041 cacctgcata atgatgagca atttcagcgg tttttaacag gccgattctg tccttaattc
     2101 tgatgggcgt gttagaaaaa aatgtggtac aacaactcag gcaacacgca aacgatttac
     2161 tcgtcgtatt tcaagttgca tgtgctgcgg ctacgttcgt tcaccccagt cacttactat
     2221 tgtaagctcc tggagttcac tcgcttgccg ccttcctgca actcgaaatc catagagtaa
     2281 ataaacatca cctttcagtg cgctattgca gccgcctgaa agaatagtgt atcgacctac
     2341 atcacagacc gcaggaaagg gtcagtataa tgattccaca gatttcccag gcacccggcg
     2401 tcgttcaact ggtgcttaat tttttgcaag agctggagca acaaggtttt accggcgatg
     2461 cggcgacaag ttatgccgat cgtctgacaa tgtcgaccga caacagtatt taccaacttc
     2521 tccccgatgc gatggtattt ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg
     2581 ccgcgcagga acgctattca tcgctgatct ttaccccccg cggcggcggc accggcacta
     2641 acggtcaggc gctcaaccag gggattattg ttgatatgtc ccgccatatg aaccgcatca
     2701 tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc cggggtgata aaagaccaac
     2761 tcaatcagta cctgaaaccg ttcggctact tttttgcgcc ggaactttcg accagcaacc
     2821 gggcaacgct cggtgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg
     2881 gcaaaacgtc agatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg
     2941 atacgcaacc tttacccgtc gagctggcgg aaacgctggg taaatccaat accacaatcg
     3001 ggcgaattta taacacggtt tatcaacgtt gccgtcagca acgccagtta atcatcgaca
     3061 acttccccaa acttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg
     3121 agatgaccga gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta
     3181 ttaccgaagc gcggctggat attacgcgct tgcctaaagt gcgccgtctg gtgaatgtca
     3241 aatatgactc ttttgactct gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc
     3301 tttcggtaga gacggtggac tcaaaagtgc tgaatctggc gcgggaagat attgtctggc
     3361 attccgtcag cgagttgatt accgatgtgc ctgacaaaga gatgctcgga ctgaacattg
     3421 tggaatttgc tggtgatgat gaggcgctga ttgacgagcg agttaatgca ctctgtgtgc
     3481 ggcttgatga gctgattgct agccaacagg caggtgtgat tggctggcag gtgtgccgcg
     3541 agctggcggg ggttgaacgt atctatgcga tgcgcaaaaa agccgttggt ctgcttggca
     3601 atgccaaagg tgccgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac
     3661 atctggccga ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagctacg
     3721 gtatgttcgg tcacgtcgac gcaggtgtct tgcacgtccg tccggcactg gatatgtgcg
     3781 atccgcaaca agagattttg atgaagcaaa tctctgatga cgtggtggcg ctgactgcga
     3841 aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccga
     3901 cgtttttcgg tgaggaactt tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc
     3961 ataaccgact caacccaggg aagatttgcc cgccagaagg cctcgatgcg ccgatgatga
     4021 aagtggacgc ggtgaagcgc ggtacattcg atcggcagat ccccattgcg gtacgccagc
     4081 agtggcgcgg tgcgatggag tgtaacggca acggtttatg cttcaacttt gatgcccgta
     4141 gtccgatgtg tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg
     4201 caacgctggt gcgtgaatgg ctgcgcttgt tggcggatcg cggcgttgac ccactcaaac
     4261 tggaacaaga actgcctgaa tccggcgtca gtttgcggac gttaattgcc cgcacgcgca
     4321 atagctggca tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt
     4381 cgggctgtct ggcctgtaaa gcttgttcga cccagtgccc catcaaaatt gatgtgccgg
     4441 agtttcgctc tcgttttctg cagctctatc acacccgtta tttacgcccg cttcgcgacc
     4501 acctcgttgc tacggtcgag agctacgcac cgctgatggc acgcgcgccg aaaaccttta
     4561 acttcttcat taaccagccg ctggtgcgca aactctcgga aaaacatatc ggcatggttg
     4621 atttgccact tctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa
     4681 acatgacgct ggaacagctt gaagctctca atgcagagca gaaagcgcgc acggtgttgg
     4741 tggtgcagga cccctttacc agctattacg atgcgcaagt ggtggcggat tttgtccgtc
     4801 tggttgaaaa attaggtttc cagcctgtgt tactgccatt ttcgccaaat ggcaaagccc
     4861 agcatatcaa aggctttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc
     4921 tcaaccgtat ggctaagctt gggatgccaa tggtgggcgt cgatccggcg ctggtacttt
     4981 gttatcgcga tgaatataaa ctggcgctgg gcgaggaacg tggcgagttt aacgtcttac
     5041 ttgcgaatga atggctggca agcgcacttg agtcacatcc agtggctaca gtcagcggtg
     5101 aatcatggta tttctttggt cactgtaccg aggttaccgc tttgccgggc gcgccagcac
     5161 aatgggccgc gatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtgggttgct
     5221 gcggcatggc agggacttac ggacatgaag cgaaaaacca tgaaaattcg ctcgggatct
     5281 atgagttatc ctggcatcag gctatgcagc gactgccgcg taaccgctgt ctggcgaccg
     5341 gatattcctg ccgtagccag gtaaaacggg ttgaaggcac gggggtacgc catcctgtgc
     5401 aggctttact ggagattatt aaatgatatg gaaacggaaa atcaccctgg aagcactgaa
     5461 tgctatgggt gaaggaaaca tggtggggtt cctggatatt cgctttgaac atattggtga
     5521 tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct
     5581 gcatggagga gcatccgtgg tactggccga aagtatcggt tccgttgccg gttatttatg
     5641 taccgaaggt gagcaaaaag tggttggtct ggaaatcaat gctaatcacg tccgctcggc
     5701 acgagaaggg cgggtgcgcg gcatatgtaa accgttgcat ctcggttcgc gtcaccaggt
     5761 ctggcagatt gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac
     5821 cgccattttg tgacgttgtc accagaaaag tgtgacgcat attgcttttg gtcattatct
     5881 gcacagtaaa cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac
     5941 tcaacttgat cccacccagc tggccattga atttttacgc cgtgaccaga gcaatctttc
     6001 acctgcccaa tatctcaaac gtctgaaaca actagaactt gaatttgctg acctgcttac
     6061 tctctcttct gctgaattaa aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg
     6121 atcctgactt aatgccgcaa gttcgccaaa tcgacctgga ttaacctccg tccaggttcg
     6181 atgaccttct taatactgct tatctaccgc ctgatattgt tcaaaaataa tactaatcgc
     6241 agtttgtgtt aaaacggcgg gttagcttta tgagattatt cttatcgccc cttcaagagc
     6301 taagccactg agagtgccgg agataagcgc cggatggggt agaaaccctt aagcctgtgt
     6361 cgcacagact taagggtttt cttatttctg aacgctgcac ttttttacct atctcttaaa
     6421 tagtcatttt caatacaact ttttcttttt catcacagcc ttaatgctcc ttagataata
     6481 accattatct aacaatgaga tacctaattc ttagcgtgcc tgttaaccca cacatcaggg
     6541 gctatgctta ttaaaagcag gcaaaaagca tgttttacgg taaagcccct gcgtttgctg
     6601 ggttgaactg ataatcatta tcactaacat gctgttatac gctgaaagcg atgaagtgag
     6661 gtaaatagat ggacatgcat tcaggaacct ttaacccaca ggatttcgcc tggcaaggct
     6721 taacgctgac acccgcagcg gcgatacaca tccgcgagct ggtggcaaag cagccaggta
     6781 tggtcggcgt gcgcttaggt gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg
     6841 acagtgttag cgagccggac aaagacgatc tgctgtttga acacgacggc gcgaagctgt
     6901 ttgtcccgct gcaatcgatg ccgtttattg atggcacgga aatcgatttc gttcgtgaag
     6961 gacttaatca gatattcaaa tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg
     7021 aaagctttag ggtataggcg gtactatgtc tcgtaatact gaagcaactg acgatgtcaa
     7081 aacctggacc ggcggcccgc tgaattataa agaaggattc ttcacccagt tagccaccga
     7141 tgagctggca aaggggataa acgaagaggt ggtgcgcgca atttcggcga agcgtaatga
     7201 gccggagtgg atgctggagt ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga
     7261 accgcactgg ttgaaagcgc actacgacaa gctgaattat caggattaca gctactactc
     7321 agcaccatcg tgcggtaatt gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca
     7381 aactggcgcg aacgcctttt taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt
     7441 tcccgtgcgg gaaggcaaag aggtggcggt ggatgccatt ttcgactctg tttcggttgc
     7501 caccacttat cgcgaaaaac tggcggagca gggaattatt ttctgttcct ttggtgaggc
     7561 gatccacgat cacccggaac tggtgcgtaa atatctcggc accgtggtgc cggggaatga
     7621 caacttcttt gcagcactta atgcggcggt agcctctgac ggtacgttta tttatgtgcc
     7681 taaaggcgtg cgctgcccga tggaactttc cacctatttt cgcattaacg cggaaaaaac
     7741 cgggcagttt gagcgcacaa ttctggtggc cgacgaagac agctacgtca gctacattga
     7801 aggctgttcc gctccggtgc gtgacagcta tcagttgcac gcggcagtgg tggaagtcat
     7861 catccataaa aacgccgagg tgaaatattc cacggtacaa aactggttcc ctggcgataa
     7921 caacaccggc ggtattctca acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag
     7981 caaaatgtca tggacgcaat cagaaaccgg gtcagcgatt acgtggaaat atcccagctg
     8041 cattttgcgc ggcgataact ccattggtga gttttactca gtggcgctga ccagcggtca
     8101 tcagcaagcg gataccggca ccaagatgat ccacatcggt aaaaacacca aatcgaccat
     8161 tatctcgaaa gggatctctg ccggacatag tcagaacagt tatcgcggct tagtgaaaat
     8221 catgccgacg gcaaccaatg cgcgcaattt cactcagtgc gactcaatgc tgattggcgc
     8281 taattgtggg gcgcatacct tcccgtatgt cgaatgtcgc aataacagcg cacaactgga
     8341 gcacgaggca acgacatcac gtattggtga agatcaactg ttttactgcc tgcaacgcgg
     8401 gatcagcgaa gaagacgcca tctcgatgat tgttaacggt ttctgcaaag acgtgttctc
     8461 agaactgccg ctggaatttg ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca
     8521 cagcgtcgga taaggaataa acatgttaag tattaaagat ttacacgtca gcgtggaaga
     8581 taaagctatc ctgcgtggat taagcctcga cgttcgtccc ggcgaagttc acgccattat
     8641 ggggccaaac ggttcgggca aaagtacttt atcggcaacg cttgccgggc gcgaagatta
     8701 tgaagtgacg ggcggcacgg ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga
     8761 agatcgtgca ggcgaaggca tctttatggc cttccagtat ccggtggaga ttccaggcgt
     8821 cagtaaccag tttttcctgc aaacggcact taatgcagtg cgcagttatc gcggccagga
     8881 aacgcacgac cgctttgatt ttcaggattt gatggaagag aaaatcgctc tcctgaagat
     8941 gccggaagat ttattaaccc gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg
     9001 caacgatatt ttgcaaatgg cggtgctgga accggagtta tgcattcttg atgagtcgga
     9061 ctccgggctg gatattgacg cattaaaagt ggtcgccgat ggcgtgaact cgctgcgtga
     9121 tggcaagcgc tcattcatca ttgttacgca ctaccaacgc attctcgact acatcaagcc
     9181 tgattacgtt catgtgctat atcagggacg aattgtgaaa tccggcgatt tcacgttggt
     9241 caaacaactg gaggagcagg gttatggctg gcttaccgaa cagcagtaat gcgctgcaac
     9301 agtggcatca cttgtttgaa gctgaaggga caaaacgctc accgcaagca cagcagcatt
     9361 tacaacaatt gctgcgtacc ggactgccga cacgtaaaca tgaaaactgg aaatatacgc
     9421 cgctggaagg gctgaccaat agtcagtttg tcagcattgc gggagatata tccccacagc
     9481 agcgtgatgc cttagcgtta acgttagacg ccgtgcggct ggtgtttgtc gatggacgtt
     9541 acgtgcccgc gctgagcgat gcaattgaag gcagcggata tgaagtgagc attaacgacg
     9601 accgtcaggg gttacccgac gctattcagg cggaagtgtt tctgcatttg acggaaagcc
     9661 tggcacaaag cgtgacgcat atcgccgtga agcgcggtca acggccggca aagccattgc
     9721 tgttaatgca tatcacccag ggcgtggcag gtgaagaggt gaacactgcc cattaccggc
     9781 atcatctgga tctggcggaa ggtgccgaag caacggtgat cgaacatttt gtcagcctta
     9841 atgatgctcg tcattttacc ggggcacggt tcactatcaa tgtcgcagcg aacgcccact
     9901 tgcagcatat caagctggcg tttgaaaacc cggtcagtca ccactttgcc cataacgatt
     9961 tgttgctggc tgacgatgcc accgcattta gccatagttt cctgctgggt ggcgcagtgt
    10021 tacgacacaa caccagtacg caactcaatg gcgaaaacag cacgctgcgg atcaatagcc
    10081 tggcgatgcc ggtgaaaaac gaggtgtgtg atacccgtac ctggctggaa cacaataaag
    10141 gtttttgtaa cagccgacag ttgcacaaaa ctatcgtcag cgacaaaggt cgcgcggtat
    10201 ttaacggttt gatcaacgtc gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca
    10261 ataatctgct gatgggcaaa ctggcggaag tggatacaaa accgcagctg gaaatctatg
    10321 cagatgatgt gaaatgcagc cacggcgcga cggtgggacg tattgatgat gaacagatgt
    10381 tctatctgcg ctcgcgcggg atcaatcagc aggatgccca gcagatgatc atttacgcct
    10441 ttgctgccga actgacggaa gcactgcgtg atgaggggct taaacagcag gtgctggccc
    10501 gaatcggtca acggctgcca ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc
    10561 cgactttccg gtgctttcgc gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc
    10621 cgccagtgca cagaaaccga gccaggtgat tgacgccgag gccgagtttt atcgtcatgg
    10681 ctacgcggcg gtgcatcgcg gtattcatac cttaagcgcc caggcgaccg agaaaatgga
    10741 gaacgtgcgc aagcgggcat cgctgtttat taatgcccgt tcggcggaag agctggtgtt
    10801 cgtccgcggc acgacggaag ggatcaatct ggtcgccaat agctggggca acagcaatgt
    10861 gcgggcgggc gataacatca tcatcagtca gatggagcac cacgctaaca ttgtcccctg
    10921 gcagatgctt tgcgcacgcg ttggcgcaga gctgcgtgtg atccctctca accccgacgg
    10981 tacgctgcaa ctggagacgc tgcctacgct gtttgatgag aaaactcgcc tgctggcaat
    11041 tactcatgtc tccaacgtgc ttggcacaga aaatccactg gcggaaatga tcacgcttgc
    11101 gcaccagcat ggcgcaaaag tgctggtgga tggcgctcag gcggtgatgc atcatccggt
    11161 ggatgttcag gcgctgggtt gcgactttta cgtgttctcc gggcataaac tgtatggccc
    11221 caccggaatt ggcattcttt atgtgaaaga agccttgttg caggagatgc cgccgtggga
    11281 agggggcggt tctatgatcg ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc
    11341 accatggcgg tttgaagccg gtacacccaa taccgggggc atcattggtc ttggcgcggc
    11401 gctggagtat gtttcggcgc tggggcttaa tagcatagcc gagtatgaac agaatctgat
    11461 gcattatgcg ctatcacagc tggaatctgt accggatctc actctctatg gccaacaaaa
    11521 caggcttggc gttattgctt ttaatctcgg taaacaccac gcctatgatg ttggcagttt
    11581 tctcgataat tacggcattg ctgtgcgtac cggacatcac tgcgctatgc cattaatggc
    11641 ctattacaac gtccctgcga tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga
    11701 agtggatcgt ctggtgaccg gcctgcaacg tattcaccgt ctgctgggat aacagggagg
    11761 cactatggct ttattgccgg ataaagaaaa attgctgcgt aattttttac gctgcgccaa
    11821 ctgggaagag aaatatctct acattattga gctgggccag cgtctgccag aattacgcgc
    11881 cgaagacgga agcccacaaa atagcattca gggctgccag agtcaggtgt ggattgtcat
    11941 gcgccagaat gcgcagggaa ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa
    12001 agggcttatt gcggtcgtct ttattctcta cgatcagatg acgccgcagg atattgtcaa
    12061 tttcgatgtg cgtccgtggt ttgaaaaaat ggcgctcacc caacatctca ccccatctcg
    12121 ttcacaaggt ctggaagcga tgattcgcgc aattcgcgcc aaagccgctg cacttagcta
    12181 aactaaaggg acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact
    12241 gacctgcaca ggagtgtttt ggtttcaggt gaacataagc accatccgac gtttcagcgt
    12301 gagtctccgg caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc
    12361 ttatcggcgc ttttagcgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa
    12421 ccggaagccg actggttggg caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc
    12481 ttcaggctat tgcccgacgt tttgatactg cggcaatgtt gatccttgaa gccaataaca
    12541 ctatcgcccc ggtgccaaaa cctggtacga cgataactat tccttcacaa ctgttattac
    12601 ctgatgcacc gcgtcagggg attatcgtta accttgcaga gctgcgcctt tattattatc
    12661 cgccgggaga aaatattgtg caggtttatc caataggtat tggattgcag gggctggaaa
    12721 cgccggtgat ggaaacgcgt gttgggcaga aaatccctaa cccaacctgg acgcctacgg
    12781 caggcattcg tcagcgttcg ctggagcgtg gcattaaatt accgccagtc gttcctgccg
    12841 gaccaaataa cccgctagga cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc
    12901 tcattcatgg caccagtgcg ccggacagcg ttggtttgcg cgtcagttca gggtgtattc
    12961 gcatgaatgc accggatatt aaagccttgt tctccagcgt gcggacggga acgccggtga
    13021 aagtgatcaa cgaaccggtg aaatattccg tggaacctaa cgggatgcgt tatgttgaag
    13081 tacatcgacc actatcagca gaagaacagc aaaacgttca gacaatgcca tacacactgc
    13141 cagcaggctt tacgcaattt aaagacaata aggctgtaga tcagaagtta gtcgataaag
    13201 cgttgtatcg tcgggcaggg tatccggttt cggtgagcag tggagcaact cccgcagcca
    13261 gcaatgcgcc ttcagtagag tcagagcaga atggtgaacc agagcaaggg aatatgttac
    13321 gcgcgacgca gtagtggtaa atagcagaca aaaaaaatgg cgcacaatgt gcgccatttt
    13381 tcacttcaca ggtactatta cttgcggtat ttagtagcca tgttgtccag acgctggtta
    13441 gcacgagctg cgtcatcttt agcagcctga acgtcggaac gcattgcgtt cacatcgttg
    13501 ctcagctggt caactttagc gttcagagtc tgaacgtcag aagacagctg atcgatttta
    13561 gcgttgctgg agcaacctgc cagcagagta gaacccagga ttaccgcgcc cagtaccagt
    13621 ttagtagctt tcattattaa taccctctag attgagttaa tctccatgta gcgttacaag
    13681 tattacacaa agttttttat gttgagaata tttttttgat gggaaggcac ttatttttga
    13741 tcgttcgctc aaagaagcat cgaacgctgg gttttagata aaaaaattga gagaaaacag
    13801 gatgcttcca tcggattcat cttaaataaa gtgagattat atagtgaaat ccgattt
//
LOCUS       DADMFL010000103        13676 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.104, whole genome shotgun sequence.
ACCESSION   DADMFL010000103 DADMFL010000000
VERSION     DADMFL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.104"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..271)
                     /locus_tag="JNZ27_003108"
     CDS             complement(<1..271)
                     /locus_tag="JNZ27_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY8665347.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAA"
     gene            complement(284..2065)
                     /gene="gcl"
                     /locus_tag="JNZ27_003109"
     CDS             complement(284..2065)
                     /gene="gcl"
                     /locus_tag="JNZ27_003109"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY8665348.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRSRLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2155..2970)
                     /gene="allR"
                     /locus_tag="JNZ27_003110"
     CDS             complement(2155..2970)
                     /gene="allR"
                     /locus_tag="JNZ27_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY8665349.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3048..3530)
                     /gene="allA"
                     /locus_tag="JNZ27_003111"
     CDS             complement(3048..3530)
                     /gene="allA"
                     /locus_tag="JNZ27_003111"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY8665350.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGGNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3760..4686
                     /gene="allS"
                     /locus_tag="JNZ27_003112"
     CDS             3760..4686
                     /gene="allS"
                     /locus_tag="JNZ27_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY8665351.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            4755..5849
                     /gene="mnmH"
                     /locus_tag="JNZ27_003113"
     CDS             4755..5849
                     /gene="mnmH"
                     /locus_tag="JNZ27_003113"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY8665352.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(6615..9029)
                     /locus_tag="JNZ27_003114"
     CDS             complement(6615..9029)
                     /locus_tag="JNZ27_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8665353.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(9026..9712)
                     /locus_tag="JNZ27_003115"
     CDS             complement(9026..9712)
                     /locus_tag="JNZ27_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8665354.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9683..10305
                     /gene="tesA"
                     /locus_tag="JNZ27_003116"
                     /pseudo
     CDS             9683..10305
                     /gene="tesA"
                     /locus_tag="JNZ27_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10295..11104
                     /gene="ybbO"
                     /locus_tag="JNZ27_003117"
     CDS             10295..11104
                     /gene="ybbO"
                     /locus_tag="JNZ27_003117"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8665355.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            11165..12019
                     /gene="cnoX"
                     /locus_tag="JNZ27_003118"
     CDS             11165..12019
                     /gene="cnoX"
                     /locus_tag="JNZ27_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY8665356.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(12082..12861)
                     /gene="fetB"
                     /locus_tag="JNZ27_003119"
     CDS             complement(12082..12861)
                     /gene="fetB"
                     /locus_tag="JNZ27_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY8665357.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12848..13525)
                     /gene="fetA"
                     /locus_tag="JNZ27_003120"
     CDS             complement(12848..13525)
                     /gene="fetA"
                     /locus_tag="JNZ27_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY8665358.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
BASE COUNT         3391 a         3726 c         3428 g         3131 t
ORIGIN      
        1 tcgctgctgc tacgccatcc cgaaactctt cttcacggcc aggaatacag gcaataccgc
       61 gctcgcccgc cgcccagtca cctgccggta aattgtgcag cgtatgttcg agtttattac
      121 tcgccagtac ctgttttaat tcttcaatgt cgtagtcata aggaaacata aattcaacgc
      181 cgcgaaaacc acactgcgca gctttctcaa aacgggcgag aaaatcatat tcaccaaata
      241 acatcgataa attagcagag aaacgtaaca tcatttatct cccttattca tagtgcatga
      301 agcaggtttc agtcggtgca tccgctgcgt tatcggcgat atcttcaaat tccatgacgt
      361 tatccagttc gctgcccatc gaaatattgg tcacacgctc gagaataact tccacgacta
      421 ccggtacccg atattgcgcc attaaggctt tcgcctgttc aaaggctggc gcaatatctt
      481 ccggtttgaa gacccgaata gctttacaac ctaaaccttc cgctactttt acgtggtcaa
      541 caccgtagcc attcacttca ctggagttga tattctcgaa agcgagttgc acgcagtagt
      601 ccatgtcaaa agcgcgttgt gactgacgaa tcaggccgag ataggcgtta ttcaccagca
      661 catggatgta cggaatgttg aactgcgcgc caacggctaa ctcttcaatc aggaactgga
      721 agtcaaagtc gccagaaatc gccaccacat tgcgtttcgg atcagcggca caacccccca
      781 gcgccgccgg aatcgtccag cctaacggac cagcctgacc acagttgatc cagtggcggt
      841 ctttaaagac atgcagcatt tgtgccgcag cgatttgtga cagaccaata gtggtgacat
      901 aacaaacatc gcgaccaaag gctttgttca tctcttcata cacgcgctgc ggtttcaccg
      961 gcacgttgtc gaagtgggtt ttgcgcagca aagtgcgttt acgctgctgg cagtcggcga
     1021 cccattcttt acgacacggc agacgacccg ctttttgcat ctcctgcgcc acttcaacca
     1081 gcagtgtcag cgccgcttta gcatcagaga caatgccgag atccggacac agcacgcgac
     1141 caatttgcgt cggctcaata tcaatatgaa cgattttgcg cccttcggtg tatttctcta
     1201 ccgaaccggt atgacggtta gcaaaacggt taccgatacc aaacaccatg tcggacgcca
     1261 gcaacgttgc gttaccgtaa cgatgcgcgg tttgcagacc gaccatcccg gccatcagtt
     1321 catgatcgtc cgggatacag ccccagccca tcagcgttgg gatcaccgga acgctggtca
     1381 gttcagcaaa ctgttgtaac agtgcagctg catcagcatt gattaccccg cccccggcaa
     1441 caatcaccgg acgttcggcc tggattaaca tttctacagc tttttcgatc tgcatacggc
     1501 tggcagcagg tttgtagacc ggcagcggtt cgtacatgtc aggatcaaac tcgatttccg
     1561 caacctgaac gtcgaacggt aaatccacca gtaccggacc cggacgacca gaacgcatca
     1621 ggtgaaatgc ctgttgcagc acgcgaggca ccagcgccgc ttcacgaact gtaaccgcca
     1681 ttttgctgac cggtttagca attgcttcaa tatctacggc ctgaaaatct tctttatgca
     1741 gacgggagcg cggtgcctgg ccggtaatgc acagaatagg aatggaatca gcagaagcgg
     1801 aatagagcgc ggtgatcatg tccgtgcccg caggaccgga agtccccaga catacgccga
     1861 tattccctgc cgttgcgcgg gtataacctt ccgccatgtg cgaaacacct tccacatgac
     1921 gcgccagaat gtgacgaata ccgccgtgct tacgcatcgc tgagtagaac ggattaattg
     1981 cagctcccgg aacgccgaag gcggtggtga taccttcttt ctccagcaca tacattgccg
     2041 cgtcaacggc tctcattttt gccattttat tcctacctct attaattgga aaaattttcc
     2101 aacttttaaa ttttgtcaaa aaaacccgct ccgcagagcg ggatgcgaca gacactatgg
     2161 atgtgctttc agtcccaacg ccgtgctgat atcgcgggcg gtgtctctga ccagctcacc
     2221 ctgactgaca aaacgatctt ctgtcagtct tgatgaaggc ccggagatgg agatagcggc
     2281 aacaacgcta ccgacatcat cgtaaattgc tgaagctatg caattcagac ctacaacatg
     2341 ctcttcttta tctacggtat agcccagttc acgcgcttgt tccaggtcct tcagcaaggt
     2401 gggcatatcc acaagcgtag ttggcgtaaa ctgctgcaaa ccggtttgca gaatgatgct
     2461 catcaactcc tcttccgcca gcggataaag cagcgctttg cccgcaccgg aagcatgcag
     2521 cggcagacga ctgcccagtg gcgcacacat cctgaccatc gatttacact ctaactgacc
     2581 aattaatacc gcttcattgc cgttacggat cgcgacattg accgtttcgc cggaaagtaa
     2641 catcaggcgg cgcataaacg gcccggcgac ggagaggaca tcgcggttat ggatgtacgc
     2701 cgcaccgacg ttaaagacac ctaatcctat atgccaccag cctaattgac tgtcctgata
     2761 gacaaaatcc gctgcttgta aaaccttcag caagcgaaag gtcgtggaga gcggcaaatc
     2821 cagattgaga gaaatatcgc taaccgacga acttccccca cttttttcca gatattgcag
     2881 aatcgcaatt ccccgctcta acgcctgtgc gcccttctgt gccacaggct ccgcctgtcc
     2941 tggcctgccg cgccgtctaa cttccgtcat acctacgtta actccgacct acaagtgagc
     3001 ataaacaatg aacaattagt ttatcttatg caagtcggtt gcaggcgtca cgcaaaacag
     3061 agttcctgtt caggaatact ttcaacatca cagttgtcac tgccgccgcg atcgatggtc
     3121 agaaaatcgg tgacgcgctg ccaggcgaaa agtgggtgat gccagacgtt acgatggtaa
     3181 ttccccccct gttcaccgtt ggtgataaac gcccgcagcg ttgacaggtc tggtttgtcg
     3241 tcgcctaacg ccacgaccac cacaaacact tcacctttca ttgggataaa ggcctgagtg
     3301 cccagcggat gacgttcgag ttcgtgaatg gtcagcggca gattcgccgg ttgcgcgcgg
     3361 ttaatgctga taagcgtacg gtcttgctca agaatctcaa ccagcgccaa atcgtggtaa
     3421 cgctccacca ggccattgtt aatatggaaa aaatcccgtt gctgcgtttc gattacgtcg
     3481 ccataagcac taaaggcttc ctgacttaac ggtaatacct gaagtttcat ctttttgtcc
     3541 ttgtaacata attccgttca accaccgccg accggtcagc ttggtttcaa taacaggcaa
     3601 tctaaaacct taagagaaca acatcaatac aaaagttgga atatttttcc aaaactagat
     3661 ttagtccaca aattgaacag gattatcatg taaaaatact gataaatcag aggataagaa
     3721 aatcacgcaa cttgcggatt taaaagggga tacctgaaga tgttcgatcc agaaaccttg
     3781 cggactttca ttgcggttgc tgaaacagga agtttttcaa aagcggcaga acgattatgt
     3841 aaaaccacgg cgacgatcag ttatcgcatt aaacttctgg aagagaatac cggagtagcg
     3901 ctgtttttcc gtacgactcg tagcgtaacg ttgacagcgg ctggcgagca tctactttcc
     3961 caggccagag actggctgag ctggctggaa agtatgccca gcgagctgca acaggtgaat
     4021 gatggcgtgg aacgccaggt gaatattgtc atcaacaacc tgctctataa cccccaggcc
     4081 gtcgcccagt tgctggcgtg gctgaatgag cgttacccct ttacccagtt tcacatctcc
     4141 cgacaaatct atatgggcgt ctgggactcg ctattgtacg aaggtttttc gctggctatc
     4201 ggggtcacgg gaactgaggc gctggcaaat acctttagtc ttgatccctt aggatcggtg
     4261 caatggcgat ttgtcatggc ggcggatcat ccgctggcga acgttgaaga gccgctaaca
     4321 gaagcgcagt tgcgtcgctt tccggcggtc aatattgaag acagcgcccg caccttaacc
     4381 aaacgcgtcg cctggcgatt gccggggcaa aaagagatta ttgttcccga tatggaaacg
     4441 aaaatcgccg cccatctggc gggtgttggc attggttttt tgccaaaatc actttgccag
     4501 tcaatgatcg ataatcaaca actggtcagc cgggtaatcc caacgatgcg ccctccttcg
     4561 ccattgagtc tggcatggcg caaatttggc agcggcaaag cggtagaaga tattgtgacc
     4621 ttgtttaccc aacacaggcc ggaaatcagc ggatttttag aaattttcgg caacccacgc
     4681 agttaaaaat caagtttcct tgcgcgttgg cgcgtaaaat agcgcgcaag ctgatttgag
     4741 gttcactttt aactatgcaa gagagacaca cggaacagga ctatcgtgcc ctgctgattg
     4801 ctgatacgcc cattattgat gttcgcgccc ctatcgagtt tgagcaaggc gcaatgcccg
     4861 ccgctatcaa tctgccgtta atgaataacg atgaacgcgc cgccgttggc acctgctata
     4921 aacagcaagg ctcagacgca gcgctggcgc tggggcataa actggtggcg ggtgaaattc
     4981 gtcagcagcg tatgtacgcc tggcgggcag cgtgcctgca aaatccgcaa ggtattctct
     5041 gctgcgcccg tggcggtcaa cgttcacata ttgtgcaacg ctggctgcat gaagcgggga
     5101 ttgattatcc gctggtggaa ggcggttata aggcactgcg ccagaccgcg attcaggcga
     5161 ccattgaact ggcacaaaaa ccgatagtgc tgattggcgg ttgtaccggc agcggtaaaa
     5221 cgctattagt gcaacaacag ccaaacggtg ttgatctgga agggctggcg cgtcatcgcg
     5281 gttcggcgtt tggtcgcacg ttacaaccac aacttagcca ggcgagtttt gaaaacctgc
     5341 tggctgccga aatgctaaaa accgacgccc gtcaggattt gcgcctgtgg gtgctggaag
     5401 atgaaagccg gatgatcggt tcgaatcacc tgccggaatg tctgcgcgag cgaatgactc
     5461 aggcggcgat tgcggtggta gaagatccgt ttgagatccg tcttgaacgc ctgaatgaag
     5521 agtatttctt gcgtatgcat catgatttta cccacgctta tggcgacgaa cagggctggc
     5581 aggaatattg cgaatacctg catcacggac tttcggcgat taagcgtcgg ctggggttac
     5641 agcgctataa cgaactcgct gcccagctgg atacggcgct gacaacgcaa ctcaccaccg
     5701 gcagcaccga cggtcatctg gcctggctgg tgccgttact taaagaatat tacgacccaa
     5761 tgtatcgcta tcagcttgag aaaaaagcgg aaaaagttgt ctttcgcggt gagtgggcgg
     5821 aagtggcggt atgggttaag gcgcagtgat gggcggtatg ggttaaggcg cagtgatggg
     5881 cggtatgggt taaggcgcag tgatgggcgg tattctgaaa cggcgattgt cgtgagaaca
     5941 aaatggttgc accagaatac agatataaaa aaccgacatg acagtcggtt tttttatttt
     6001 ttccgcccaa gacttggttg ttccgggcag aaattaaaca gtcctcatct agcaagtgaa
     6061 ttataagtca ggatgcctac atattcaaac agctatgttg gcgatacaat cccgacgcta
     6121 gtagtgatac tgcctcaagg gattaccgca gtgcggaaaa gtacttttaa gtcctggtca
     6181 ggatacaggt ggtgctgatt ctggtgctct aatttggaga gaggcagtcc tcatctagca
     6241 catgaagaaa atcatagtta caggcaacgt aagggtaagc tgtgattgtc tttctccctt
     6301 aagggagcaa cggatgaaaa cttttctcga taaagttctc atcgttctta aggcgctaat
     6361 cgcgctgctg gaactgatcc gtcagtttat tgaataactg aggaaacgta gctaaggccg
     6421 aaggtttacc tccttcggcc tttaaaaaaa ccgacatgac agttggtttc atatttattg
     6481 ccaaaatgca cactgttcat gccagatgcg gcgtaaacgc cttatccggc ctacacaatc
     6541 ttgcaaaatc aatatattgc acgctcttcg taggcctgat aagcgtagcg catcaggcaa
     6601 tctaacacct gtcatcaccc cacaaactgc ctgaacagcg ccttaccctt aaccagtcgc
     6661 gcacccagcc agccgccgaa aagcgacagc agcagcgcac cgctgcacgg cagaacaatc
     6721 cacaatcgcc agtctggctc ccacgggaaa tcaaacactt tcgcctgcaa cactgccagt
     6781 gccgtttccg caccaattgc ggccaccagg ccggaaacaa acccaagcat ggcgaactca
     6841 caccacaatg tcgtgcgcag cagttttttc cccgcaccga gtgtgcgcca taccaccagc
     6901 tcctgatgac gctggcgcat tcccacctgc acctgtgcca gcaacagcaa cataccgcag
     6961 gcggtgacca gcaccaccat cacctccagc gcccgactta cctgctccag cacctgaccg
     7021 acctgtttta aaatcgcgcc aatgtctaac aggctaatgg tcgggaactg gcggttgagt
     7081 tgcgtcaaca tgccgttgcc attctcccag cggaaactgg taagccagct ctgcggttgc
     7141 ccgtctaatg ccccttcagg gaaaataaaa tagaaattag gccgcagact ttcccagtcc
     7201 actttgcgca ggctggtcac tttagcgcgg aactcctggg tatcgcccat aaaagtcacg
     7261 gtatcaccga gggcaacgtt taagcgtttc gccagcccct cttccatcga cacttcatcg
     7321 gcttttggcg gccagttacc ggcgacaatc ggattatgat cgggccgcgt attttgccag
     7381 gtaagattga gttcgcgatt aagcgcctca tcttcattac cttctgtcgg cttatcgtta
     7441 atcgccgtca gccgcgcccg cactaccgga taaaatgatt ccgggactat atgatgttcc
     7501 gcgaggaacg cttttagcgg tgctacctgt tctgtggcga tgttaattaa aaagtagttc
     7561 gggctttctg gcggtagctg ctgctgccag cggtcgagca gatcgccacg cagcaccagc
     7621 agcagtgcca gcagcataaa ggagagcgaa aatgccgaaa gctggcttaa cgttgaccac
     7681 ggctgacgta acaggcggct aaccgccagg cgcagaggca gcgatttcag cgtcatgcgg
     7741 cgaagtacat tcagcagcat ccagcccagc acaccgcaca gcaaagccag tactaccgcg
     7801 cccgccagca ccgcccaaag cagcatgctg ccacccatta atccggcgag cagcagcaca
     7861 accaccacac tgacaatcgg cagataaaac ttcagcggcc agacgttcgc gaccacatca
     7921 ttacgtaata cgcgtaaagg ctgcgttgcc agcaacaagc ggtatggtcg tagccccacc
     7981 agcagcgaga tgaccgtcat ggtgccaagc gcccacagcc acggccagag gctggctggc
     8041 ggtagtgcag caggcagaac gggcttgagc agcaccatca acacgttttc gaacaacagg
     8101 cctatggccc caccggtaac ggctgaaagc gtcagcacca tcaaccactg accgacgatt
     8161 agcttacgca gttgcgctcg ccctgccccc agcgttttga ggatcgccac cagatcgtag
     8221 cgactgcgac agtaatgatt catcgctacc gccaccgctg ccactgccag cagcaaggtc
     8281 agaagcgccg aaagcagcag gaactgttgc gagcgttcca tcgatcgccc cagcgcgcct
     8341 tcgtcctgtt ccagaccgta ccagcgttgt tcgggtttaa gctgaggtaa caaccatttc
     8401 tcatagccgt cgagctggtt ctcgttgccg ccgaatttat aacgccaggt gacccgactc
     8461 cccggctgca cggctccggt tttatcgaca tccgccagat tcatcatcag acgcggagcc
     8521 atctggaagg ggttaaaacc ggaatccggt tcctgaatca cttctccggc aatccgcaag
     8581 gtggcatcgc ccacgtcaat ggtgtcgtcc gttttcaggt taagcagtgc catcaggcgt
     8641 ggggccagca atacgctgcc cgcctgcggt ttcaggccag ggggattagt ttgcagatcg
     8701 ccatacatcg ggtagatatc atccaccgct ttgacgttcg ccagctgcgg tgtgtcgcct
     8761 gcaaaggtca ttgtggcgaa agtcagctgc ttgccgactt tcaggccgcg cttttgcgct
     8821 tcctccagcc acgcttgcgg cacttcgcgt gaactgcgca acgcccgatc gcccgccata
     8881 aactcacggc tttgctggct taagcccttc tccatgcgat cgctgatatt gcccagcgcc
     8941 agcacgcagg ccaccgccag gcttagcgcc agccagacaa ttaatagcga cggcgaacgc
     9001 cattcgcgcc agaaccaacg tgcaatcatg cttcctcctg caactgcccg ttcaccagcc
     9061 gtaagcagcg gtcgcagcgt gccgccagtt gcaggtcgtg ggtcaccata atcaacgtgg
     9121 tgccatgttc acggttgagg gaaaacagca ggtcggcaat tttatcgccc gtctggcggt
     9181 caaggttgcc ggtgggttcg tcggcaaaca gcacatcagg tcgaccatta aaggctcgtg
     9241 ccagcgccac tcgttgctgt tcaccgccgg aaagctgtgc cggaagatga tccagacgtt
     9301 tacccagccc taactgttcg agcaacgctt tcgccccgtt acgactttcc gcgctactct
     9361 caccgcgcag cagagccgga agctcgacgt tttccagcgc gttaagggta ggaattaaca
     9421 taaatgactg aaaaacaaag ccgacgtgct tcgcgcgcaa ctttgcccgc gcttcttcgt
     9481 ccatattatg tagcggttgt cccaccagac tcacttcgcc actgctgccg tcatcaagcc
     9541 cggcgaggat cgccagcaag gttgacttac ccgatcccga ctcgcccacc agtgcgatgg
     9601 tctcgccacg tttgacaacc agctcaactc cggtgaggat ggagagttca tgctccccct
     9661 gaccgacgga cttcttaaga tgatgaactt caacaatgtt ttccgctggc atttgccctt
     9721 cctgttcctg gtcctgttaa ccttccgtgc cgccgcagcg gacacgttat tgattctggg
     9781 tgatagcctg agcgccggat atcgaatgtc tgccagcgcg gcctggcctg ccttgttgaa
     9841 tgataagtgg cagagtaaaa cgtcggtagt taatgccagc atcagcggcg acacctcgca
     9901 acaagggctg gcgcgccttc cggctctgct caaacagcat cagccgcgtt gggtgctggt
     9961 tgaactgggc gcaatgatgg tttgcgtggt tttcagccac agcaaaccga gcaaacgctg
    10021 cgccagattt tgcaggatgt caaagccgcc aacgctgaac cattgttaat gcaaatacgt
    10081 ctgcctgcaa actatggtcg ccgttataat gaagccttta gcgccattta ccccaaactc
    10141 gccaaagagt ttgatgttcc gctgctgccc ttttttatgg aagaggtcta cctcaagcca
    10201 caatggatgc aggatgacgg tattcatccc aaccgcgacg cccagccgtt tattgccgac
    10261 tggatggcga agcagttgca gcctttagta aatcatgact cataaagcaa cggagatcct
    10321 gacaggtaaa gttatgcaaa aatcggtctt aattaccgga tgttccagtg gaattggcct
    10381 ggaaagcgcg ctcgaattaa aacgccaggg ttttcatgtg ctggcaggtt gccgaaaacc
    10441 gaatgatgtt gagcgtatga acaacatggg atttaccggc gtgttgatcg atctggattc
    10501 accagaaagt gttaatcgcg cagcagacga ggtgatcgcc ctgaccgata attgtctgta
    10561 tgggatcttt aacaatgccg gattcggcat gtatggcccc ctttccacca tcagccgtgc
    10621 gcagatggaa cagcagtttt ccgccaactt tttcggcgca caccagctca ccatgcgcct
    10681 gttacccgcg atgttaccgc acggtgaagg gcgtattgtg atgacatcat cggtgatggg
    10741 attaatctcc acgccgggtc gtggcgctta cgcggccagt aaatatgcgc tggaggcgtg
    10801 gtcagacgcg ctgcgcatgg agctacgcca cagcggaatt aaagtcagcc tgatcgaacc
    10861 cggtcccatt cgtactcgct tcaccgacaa cgtcaaccag acgcaaagtg ataaaccagt
    10921 cgaaaatccc ggcatcgccg cccgctttac gttgggaccg gaagcggtgg tcgacaaagt
    10981 gcgccatgct tttattagcg agaagccgaa gatgcgctat ccggtaacgc tggtgacctg
    11041 ggcagtaatg gtgctcgagc gcctgctgcc tgggcgcgtg atggacaaaa tattgcaggg
    11101 gtgagttgaa gcgcgcgctt aagcccccat gtcaaagaaa atatcgacaa cagagagcga
    11161 ctccatgtcc gtagaaaata ttgtcaacat taacgaatct aacctgcaac aggttcttga
    11221 acagtcgatg accactccgg tgctgttcta tttttggtct gaacgtagcc agcactgttt
    11281 gcagttaacc ccgatcctgg aaagcctcgc ggcgcagtac aacgggcaat ttattctggc
    11341 gaagctggac tgcgacgcgg agcagatgat tgccgcccag tttggtctgc gcgcgattcc
    11401 gaccgtgtat ctgttccaga acgggcaacc ggtagatggc ttccaggggc cgcaaccgga
    11461 agaggcgatc cgcgccctgc tggataaagt gctgccgcgc gaagaagagc tgaaagcgca
    11521 gcaggcgatg caactgatgc aggaaggcaa ttacaccgat gccctgccat tgctgaaaga
    11581 cgcctggcag ttgtcgaatc agaacgggga gatcggcctg ctgctggcag aaacgctgat
    11641 tgcgctgaac cgttctgaag atgcggaagc ggtgctgaaa accattccgt tgcaggatca
    11701 ggacacccgc taccaggggc tggtagcgca aatcgaactg ctgaagcagg cggctgataa
    11761 gccggaaatt caacagttgc aacagcaggt ggcggagaat ccagaagatg ccgcactggc
    11821 gacgcaactg gcgctgcaac tgcatcaggt tgggcgcaat gaagaggcgc tggagttgct
    11881 gttcgggcat ctgcgtaaag atctcaccgc cgcagacggt cagacgcgta aaacgttcca
    11941 ggagatcctc gctgcgctgg gtacgggtga tgcactggcg tcgaagtatc gccgccagct
    12001 gtatgcattg ttgtattgat attccgttgt tcggcacatg aaccacaacg ccggatgcga
    12061 tgctgcgcat ccggcatcgc atcatttctt cttcaattga gtcaccacca actggtggcg
    12121 cgaattataa aacttacgat aggttaaata gcaggcaata atggtcgaca agctggcggt
    12181 tgagagcagc ataaaggtca ccataatctg atatttaatc gccttcaccg gatcaatccc
    12241 ggcaaatatc agcccggaca tcattcctgg taaactcact aagccaaccg tttttgctga
    12301 atcgaccgtc ggaattaaag ccgcgcgaat actgtcgcga atcaatatcg ctgaagcctg
    12361 cttcggcgtc gcgccaagac tcagtttctc ctgaatctgt tgctgttcgc taatgacccg
    12421 ttgccctaaa ttgttgtaac acaaccccac cgctaccatg gcgttaccgg caatcatccc
    12481 ggcgacaggg atcacctgca tcgggataaa ttcaatcgac cctgagagaa tcagcaccgc
    12541 cagggtaatt cccgccccga ccgtaatggc aataaacgat gagataaaag ctttagcaat
    12601 atatttactg cgtttttgcg cgttccacgc cgcattaaag cagataaata acaccatcaa
    12661 taatgtcagg ctggcatcat ccacgctgaa aatatacttc agcacatagc cgacaataat
    12721 cagctgaatt atcgctcgcc cgacgctcca gagaatatct ttctccagcg ccagtttttc
    12781 tttatggcta attaagattg ccaccaccac cagcattaat gccagtgcta atgattcgtt
    12841 agtaatatta tgcgagttca tagcgtactt cctgcatttc tccggcatgc ggttgcagtg
    12901 taatcacttt atccgcatga ttaatttcgt ctttatcgtg tgtcacccac agcacggcaa
    12961 tattttgctc gcgcacataa cgatggatca tctcattgac gttatgttta ttactttcat
    13021 ccagcgcact ggttatttca tccagcaata aaaccttcgg cataaattgc aggttacgaa
    13081 tcaatgagat gcgttgtttt tcaccaccag atagctcggc gatattcttc gtcaaaatgc
    13141 tgtccggcaa agcgaagcgt tcgagaaaat cgagaaaaat ggccgggtca ggctgctggt
    13201 cacggatctg ccagggaaag atcagattat cgtataccgt atcgccaaac agcgtcggcg
    13261 tctgggcgca gtaagagact tgctggcggt agatttctgg ttttagtgtg ctgacatcct
    13321 caccttcaaa cagtaacgtt ccgctggttg ggctgatcaa tgaagcaact atttttagca
    13381 gcgtactttt gccacaacca gaaggaccgg taattaactt aaattcgcca gcacgcagcg
    13441 aaaaattgat gttattaaga atcttcgtat cacccgccag atatcctacg ttttgtagct
    13501 gaagcaaagg actattttcc tgcatcgctg ttcccttttt ctgattttta ctaaaaacag
    13561 tttatccttc gcaggaataa gggggaactc tctttcagta atcaggtata atttgtttaa
    13621 tcaagcagca attttgctga ttatggacgg tacaacagga ggtttttccg atgctt
//
LOCUS       DADMFL010000104        13640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.105, whole genome shotgun sequence.
ACCESSION   DADMFL010000104 DADMFL010000000
VERSION     DADMFL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.105"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            180..383
                     /locus_tag="JNZ27_003121"
     CDS             180..383
                     /locus_tag="JNZ27_003121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665359.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            401..721
                     /locus_tag="JNZ27_003122"
     CDS             401..721
                     /locus_tag="JNZ27_003122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665360.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDYVYD
                     WYAEKNGHFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(787..1019)
                     /locus_tag="JNZ27_003123"
                     /pseudo
     CDS             complement(787..1019)
                     /locus_tag="JNZ27_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(1129..1899)
                     /gene="yafV"
                     /locus_tag="JNZ27_003124"
     CDS             complement(1129..1899)
                     /gene="yafV"
                     /locus_tag="JNZ27_003124"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY8665361.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            2053..2526
                     /gene="ivy"
                     /locus_tag="JNZ27_003125"
     CDS             2053..2526
                     /gene="ivy"
                     /locus_tag="JNZ27_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY8665362.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2569..5013)
                     /locus_tag="JNZ27_003126"
     CDS             complement(2569..5013)
                     /locus_tag="JNZ27_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY8665363.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIGYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTCGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5252..5830
                     /gene="lpcA"
                     /locus_tag="JNZ27_003127"
     CDS             5252..5830
                     /gene="lpcA"
                     /locus_tag="JNZ27_003127"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY8665364.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            6036..6803
                     /locus_tag="JNZ27_003128"
     CDS             6036..6803
                     /locus_tag="JNZ27_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY8665365.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPCFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6774..7514)
                     /locus_tag="JNZ27_003129"
     CDS             complement(6774..7514)
                     /locus_tag="JNZ27_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY8665366.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7806..8419
                     /locus_tag="JNZ27_003130"
                     /pseudo
     CDS             7806..8419
                     /locus_tag="JNZ27_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8595..9092
                     /gene="rayT"
                     /locus_tag="JNZ27_003131"
     CDS             8595..9092
                     /gene="rayT"
                     /locus_tag="JNZ27_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY8665367.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(9309..11048)
                     /gene="flhA"
                     /locus_tag="JNZ27_003132"
     CDS             complement(9309..11048)
                     /gene="flhA"
                     /locus_tag="JNZ27_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY8665368.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSMLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            10993..11778
                     /gene="lafU"
                     /locus_tag="JNZ27_003133"
     CDS             10993..11778
                     /gene="lafU"
                     /locus_tag="JNZ27_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY8665369.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11849..12904
                     /gene="dinB"
                     /locus_tag="JNZ27_003134"
     CDS             11849..12904
                     /gene="dinB"
                     /locus_tag="JNZ27_003134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY8665370.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12956..13249
                     /gene="yafN"
                     /locus_tag="JNZ27_003135"
     CDS             12956..13249
                     /gene="yafN"
                     /locus_tag="JNZ27_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY8665371.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            13252..>13640
                     /gene="yafO"
                     /locus_tag="JNZ27_003136"
     CDS             13252..>13640
                     /gene="yafO"
                     /locus_tag="JNZ27_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY8665372.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         3357 a         3517 c         3492 g         3274 t
ORIGIN      
        1 atggtaacta taaaggtggc ggaattggac agccaaagag tggcacaata tcagcacagg
       61 acattattga tcatctctaa gaaaaaggtg atgggcgttt aacatctttt agtactaaaa
      121 aaacatctag ttccaactct cgtggtgcag attttttctc aaaaaaccat gtcttcaaag
      181 tggcagtttc tgatctcgat gatttagtta aaaataatca gatcactgtt ttaacacaag
      241 aaaaagtaaa agcaatattt ggtttagaat caagcaagaa aattagaaaa gaacttggta
      301 atattcttca gaatatggct aggaataatg aggtgttagt tcatgggcaa attcttggaa
      361 ataaagttag ccgatgtaat tagttagtgg agaggaagtt gtggacgaaa ttagaataag
      421 aattgctggt aatacggctg cgccttgttt ccatgtgcta aaaaataagg gatataaagt
      481 aacaatggaa actcatgcat tatctgacga ttgtttagat tatgtatatg attggtatgc
      541 ggaaaaaaat ggacactttt ttagtgcaac gagtccagaa gagttacttg gattagttgc
      601 aatgtgggaa gttcgtggta atgactggag agctaatttg aatgagataa atacttatca
      661 aaaagccata gaagaatccc cgttttttga taaaaacggc aatgaaatag agtctgacta
      721 aattttattg atatagcaac gataataacc ggaaatttct tccagatctt tccggctttt
      781 tattatttaa ctttcgttca gcacactaaa catcccgtct cgcatcccct taactgactg
      841 tttaacctga taaagttccc tgcgcagttc ctgtacttcg ttgttatccc aagcaggatc
      901 aaacatccct ccgagatctt cacggtaacg cccgtgtcga tctcaaatcc cgcttttctc
      961 agccatttgg cgctgatttt gatcaccggc gtgtcgctgc ggttgggcag ataacccacg
     1021 gtacagcgac gctgtgtttt ggaaatggtg acttctggcg taatattgtg tttaagttag
     1081 gggctttcgt aatctagccc agcccccggc cctaaacaca acccccactc accacaacct
     1141 aaactcatcc gcatcccgcc atgccggaaa cttttctcta tattcccgca atgccaccat
     1201 cgacagctcc gcatcaatac gcgttgcctt atgcgcgtcg gcggtagcga taatctcgcc
     1261 ttgcggatta atcacccggc tgtcaccgcg ataatggcag ccgttgccat cgctgccgac
     1321 gcgattgcat cccgccacat acgcctgatt ctcaatcgcg cgggccgtca gcaatgcctg
     1381 ccagtgcaga gagcgcggag ctggccagtt ggcgacgtac agggcgaggt cataatcgtt
     1441 gagattgcgc gaccacactg gaaaacgtaa gtcgtagcac accagcggca aaatacgcca
     1501 gccgcgccat tccacaatca ctcgcgcatt gcccgcttta taatgcagat gctcatctgc
     1561 catgcggaac agatgacgct tatcataaaa atgtaccgtg ccgcccggct caaccagcaa
     1621 aaagcggtta accgaaccag actccgtttg taatgcaaca ctgcctgcaa tcagcgcatt
     1681 gcactgctgc gccttagctg tcatccagtt cactacgtca tcttgtgcaa gcgacgaagc
     1741 tgccgcttcc atggcaaagc cgctggtaaa catctccggt agaacgatca catcgcgccc
     1801 ggtaatacct tccagttgac gatcaaaatg gcgcaggttg gcaggaccat ccacccacac
     1861 caacggttgc tgcaaaagcg taatcttcaa actaggcacg gtgtacaact cctttatgcg
     1921 aagggtttta taactttaac accttatcag gcagttgcct tagcgcagaa taaattgata
     1981 acaaatgctg atattggaaa tatctgattt gcaaattatc gtgttatcgc caggctttag
     2041 gaggttaata acatgggcag gataagctcg ggaggaatga tgtttaaggc aataacgaca
     2101 gtcgccgcac tggtcatcgc caccagtgca atggcgcagg atgatttaac cattagcagc
     2161 cttgcaaagg gcgaaaccac caaagctgcg tttaatcaga tggtgcaagg gcataagcta
     2221 cctgcatggg tgatgaaagg cggtacttat actcccgcac aaaccgtaac gttgggagat
     2281 gagacgtatc aggtgatgag cgcgtgcaaa ccgcatgact gtggctcgca acgtatcgct
     2341 gtgatgtggt ccgagaaatc taatcagatg acggggctgt tctcggctat tgatgagaaa
     2401 acgtcgcaag agaaactcac ctggctgaat gtgaacgatg cgctttcgat tgatggtaaa
     2461 acggtgctgt tcgcggcgtt gaccggcagc ctggaaaacc atccggatgg ctttaatttt
     2521 aaataattag cggataaaga aacggagcct ttcggctccg ttattcaatt acgcggcttc
     2581 aactttccgc actttctccg gcaactttac cggcttcgtc gccagctctt ccggatcaaa
     2641 gtcatcaacg ttaatactgc gcagacggct ttcttccgct ttcaccagaa tagcggcttc
     2701 atctttatca atcagcccct tcgtcagcgc gttgtgcgcc agttcatcca gacgggtaaa
     2761 tggcagattt ttacccagct ctttacagat ccgctgatga atcgagtcgg cggcaatcac
     2821 atccaccaac gcctcttcca gtaaaccaac cgggttatgc tcgcttggcg tcaggtactg
     2881 accgcgacca atacgtgaac gagtggcgtt cggcacttgt aaaatcttcg ccactttatg
     2941 atccagcttg tcagacggtg ccagataatg acgtccggtc gggaagatca ccacattcag
     3001 cagcccggca accacgcggt tcgggaagtt ttgcagtaaa tcgtccatcg cctgttctgc
     3061 ctgatacagc gcatcctgta cgccccagtg caccagcggc agatcggctt cattacggcc
     3121 ttcgtcgtca taacgcttca gaacggcaga ggcgaggtaa agctggctta aaatatcccc
     3181 cagacgtgcc gagatacgct cacgacgttt caggctaccg cccagcagtg ccatcgagac
     3241 atcagaaagc agggcgaggt tggcgctcag gcggttcagg tgctgatagt agcgtttagt
     3301 ggcatcgccg gttggcgtgc tgctggttaa accgcacgtc aggcccagcc agaagctgcg
     3361 aactttgttg ctaccgacgt gaccgatatg tttgaacaac agtttatcga acgcgttgac
     3421 gtcattgttc ttcgccgctt ccatctcttc cagcacgtac ggatggcaac gaatcgctcc
     3481 ttgtccgaag atcatcatgc tgcgggtcag aatgttagcc ccttcaacgg tgatggcaat
     3541 cggtgcgccc tggtaagcac gcgccaggaa gttgctttgc ccgagcataa tgcctttacc
     3601 gccggtaata tccatcgcat caataatcga ctgctgcccg cggtgggtac agtgatactt
     3661 aacgatagcc gacagcacgg caggtttttc gccgagcata atgccgtagg taatcagcga
     3721 tgccgcagca tccattacat aagcgttccc ggcaatacgc gccagcggct cttcaatccc
     3781 ttccatctta ccgatagaga ttttgaactg acggcgaatg tgagaatacg cgcccgttgc
     3841 cagcgctacc gatttcacgc cgccggttga gttggaaggc agggtgatgc cgcggcctac
     3901 cgagaggcac tccaccagca tccgccagcc ttgcccggcc attttcggcc cgccgattat
     3961 gtaaccgatc ggcacgaaga catctttacc gcgcgtcggt ccgttctgga acggtacgtt
     4021 cagcgggaag tggcgacgac caatttccac gcccggcgtg gtggttggga tcagcgcaca
     4081 ggtaatgcct aaatcttctg caccgccgag taatttttcc gggtcggaga gtttaaacgc
     4141 cagcccaagc acggtcgcaa tcggtgccag cgtaatgtag cgtttgttcc aggtcagacg
     4201 catccccagc acctgctggc cctgccattc gcccatgcag acaatcccgg tgtccggaat
     4261 cgcgcccgca tcggaacccg cttccgggct ggtcagtgca aagcagggga tctcctgacc
     4321 acgcgccaga cgcggcagat agtgatcttt ctgctcgtca gtgccgtaat gttgcaacag
     4381 ttcgcccggg cctaatgagt ttggcacgcc gacggtaatc gccaggatcc cgctcacgcc
     4441 agagagtttt tgcagcacac gagactgggc ataagctgag aactccagtc cgccgtactc
     4501 ttttttgata atcatcgcga agaagcgatg ctctttaagg tacgcccaca actccggcgg
     4561 cagatccgcc agctcatgag taatctggaa atcattcgcc atccggcagg cttcttctac
     4621 cgggccgtcg agaaacgctt gctcttcagc ggtcaggcgc ggctgcggat agttatgcag
     4681 ctttttccag tccggcttgc cctggaacaa gtcgccctcc caccaggtgg tgcccgcatc
     4741 aatcgcttct ttctcagtgc gcgacatcgg cggcatcacc ttacggaaac cgcgaaatac
     4801 cggcgcggaa atcatcgact tacgcatagg cgcaaagtta aatggcacga ggataatggt
     4861 cagaggcacc agtacccacg ccgaccacag accagcaacg ccgagggcgg ctgtccaggc
     4921 gagcaaaatc agactgctga taaataagct cacgcggtga tagaacaacg cgccgagcag
     4981 gacaaccgta gcgagaatac tcaaaatcat cataacgaaa agccccttac ttgtaggagg
     5041 tctgaccact gtgatgatat ggttgtagtg gatgtaaaaa catttagcaa tgtgtttaca
     5101 atataattac aacaaagctc acattgttgc tgtttttatc cgcacttcag gtcaaaaagt
     5161 cctggtcata gcacctgccc gtacttctcg cttttggcgg tatccggtac actgcatttt
     5221 gtctattaca tttatgctga aggatatcct catgtaccag gatcttattc gtaacgaact
     5281 gaacgaagcg gcggaaacgc tggctaactt tttaaaagat gacgccaata ttcacgccat
     5341 tcagcgcgcg gcggtcctgt tagcagacag ctttaaagcc ggtggcaaag tgctttcctg
     5401 cggcaacggc ggttcccatt gcgacgctat gcactttgcc gaagagttga ccggtcgcta
     5461 ccgtgaaaac cgtccgggct acccggcgat tgctatttct gacgttagtc atatttcctg
     5521 cgtcggtaat gatttcggtt tcaatgatat tttctcccgc tacgttgaag cggtaggtcg
     5581 cgaaggcgat gtactgctgg ggatctccac ctccggtaac tctgcaaacg tgatcaaagc
     5641 gatcgcagcg gcgcgtgaga aggggatgaa agtgatcacc ctgaccggta aagacggcgg
     5701 taaaatggct ggcacggcgg atatcgaaat tcgcgttccg cactttggtt atgccgaccg
     5761 cattcaggag attcacatta aagtgatcca tatcctgatc cagttaattg aaaaagagat
     5821 ggttaagtaa gtctggcgta ggccggataa ggcgtttacg ccgcatccgg cacttgtgcg
     5881 ctgatgcctg atgcgatgct gacgcgtctt atcatgccta caaatctgta cccgaaccgt
     5941 aggccgaata attcgtttac gccgcatccg acctgaaaat tcttaaatca atcttcgccg
     6001 gggtccatgc gctcccgctg ttgtggaggt tacccatgtg cgaattgctc gggatgagcg
     6061 ccaacgtccc taccgatatc tgctttagtt tcaccggact tgtacagcgt ggtggtggaa
     6121 ccgggccaca taaagatggc tggggcatta ccttttacga aggtaaaggc tgtcgcacat
     6181 ttaaagatcc acaaccctgc tttaattccc ccatcgccaa acttgtccag gactacccga
     6241 taaaatcctg ttcggtggtg gctcatattc gccaggctaa tcggggcgag gtggcgctgg
     6301 aaaatactca cccatttacc cgcgagttat gggggcgtaa ctggacttat gcccataacg
     6361 gacaactgac gggctacaaa tcactggaaa ccggcaactt ccgcccggtc ggtgaaaccg
     6421 acagcgaaaa agccttttgc tggctgctgc ataaattaac gcagcgttac ccgcgcacgc
     6481 cgggcaacat ggcggcagtg tttaaatata tcgcctcact ggcggatgaa ctgcgggcga
     6541 agggcgtttt caacatgctg ctttcggacg ggcgctatgt aatggcgtat tgctcgacta
     6601 atttacactg gatcacccgc cgcgcgccgt ttggcgtggc aacgttgctg gatcaggatg
     6661 tggaaatcga cttcagctcg cagaccacac cgaatgatgt ggtcacggtg attgcgacac
     6721 agccgctgac gggcaatgaa acctggcaaa aaattatgcc aggcgaatgg cgcttatttt
     6781 gcctcgggga gcgtgtagtt tgatgccagt tgtggctgca ccacttcgtg gcttaacggc
     6841 ttgctgacca cgtaacggcc attggccaca gaaacggtcg gtggcttacg ggtttgctca
     6901 aagtagtcgt agcccggctt cagctgctcc cagaagtcct taaagttgga atatttatgg
     6961 cgcttcatat tggcgtcggt catgcggaat gggtaaatac tcacctgcac gctcggctga
     7021 ccaaacacca gcgcaccagt aacgaactgg aagatctcat caataccctg attggtcatt
     7081 gcgtagcagc cgatggatac acaatcgccg tggatcatca ggtatttccc ttcataacca
     7141 tgcgcacggt cataagcatt ggggaatcca atattaatcg ctttgtagta acggctgtct
     7201 ggttttaact gattacgctg gacgctataa aacccttccg ggcttttgaa atcgccctga
     7261 cgctgttttg gccctaagcc gccggaatat ttacagattt tataactgtc gagcagttga
     7321 tattgctcgc ccattttgac gtaaagatcg agcgtacgtt cttccttgaa gatctggatg
     7381 tagacagggg atcccatcaa ctgctgctta tactctttgc tcactggcgt ggtcgagcta
     7441 ctgctgccca gcaaaccggc aaacgaaacg cacgggatca acaacatcgc aagaattaat
     7501 gcgattttac gcatactgct tattccttga taaaacggtt acacacgcca ggacggcaaa
     7561 atggatccca aatcggaata gtctggattt ggaaggctca cattatcacc aaaagagttt
     7621 tacgcaagcc tgtcggcgct gggtttacaa atttagcaca ggggcagttt tcaatagtcc
     7681 gaaaagcgca tgtatcattt atttcctaat attatgaaat ctcaatagta atatgcaaat
     7741 aagcaattaa taatcccgtt tactcctccg gtcttttaaa attctcacct tcattcagcc
     7801 ggtttatgtc tttcatgtct tcgttcttgc tcggcagatt tttgcatcca ggcgtgtttt
     7861 cgttatgcgt tttgctccct ttactcgcca gcgccaccac aaccgggagc agataaaaca
     7921 gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac agacgctggc aggagctggc
     7981 ggaaaacaac cactttttaa gcagcgatca cctgcataac atcaccgaag tggcgattca
     8041 ccgcctggag cagcagcttg gcaagcctta cgtctggggc ggcacgcggc ccgatcaagg
     8101 cttcgattgt agtgggttag ttttttatgc ctacaacaag atccttgagg cgaaactccc
     8161 gcgcacggcc aatgagatgt accactaccg tcgggcaacg attgtggcga ataacgacct
     8221 gcgccgggga gatttgctat ttttccatat ccacagccgc gagattgccg accatatggg
     8281 ggtgtatctg ggcgacggtc agtttattga gtcactgcgc accggggaaa ccattcgggt
     8341 aagccggtta gccgaacctt tctggcagga ccatttttta ggcgcacgca ggattttgac
     8401 ggaagagatg attttgtagg ccggataagg cgtttacgcc gcatccggcg gttgtgcacg
     8461 gatgcctgat gcgacgctga cgcgtcttat catgcctaca aatctgtgcg tttttgcgag
     8521 ccgctttccc gatataacac agcgtcattg tcggcctttc cttttcctct taccgttgag
     8581 aaaaaggagt cgctatgtcg gaatatcgtc gttattacat caagggggga acatggtttt
     8641 tcacggtaaa tttacgcaat cgtcgaagcc atcttctgac cacccagttc cagacgctcc
     8701 gtaacgccat tattaacgtt aagcgagaca gaccttttga aattaacgcc tgggtcgttt
     8761 tgccggagca tatgcattgc atatggacat tacccgaaag cgatgatgat ttttcctctc
     8821 gctggcggga gattaaaaag cagttcaccc atgcttgcgg attgaaaaat atctggcaac
     8881 cgcgtttttg ggagcatgcc attcgcaata caaaagatta tcgccatcat gttgattata
     8941 tttatataaa tccagagaaa catggctggg taaagcaggt gagtgattgg ccattctcaa
     9001 cgttccatcg cgatgtcgcg cgagggttat atcccatcga ttgggcggga gacataacgg
     9061 atttaagtgc cggggagcgt atcattttat aactgtgcgc tggtgccgga tgcgcttacg
     9121 cttatccggc ctacaaatcg tgctttaacg gtaggccgga taaggcgttc acgccgcatc
     9181 cggcagttat gcgcagatgc ctgatgcgac gctggcgcgt cttatcatgc ctaaaaactt
     9241 gtgcctgaac ggtaggccgg ataaggcgtt tacgccgcat ccggcaattg tgcaccgatg
     9301 ccggattctt acatcaacgc ccccataatc ttcaactcca gctcatccgg cacttcgtta
     9361 tacgacagca catgcagccc cggcgcaaac aaccttgcat aacgcgccag caaagggcgt
     9421 agctgcggcg gcaccagcag caccggatct ttccccgccg ctttcatctg ctctttcacc
     9481 tgtggcatcg tgctctggaa ctggttgagc atattcgggt caaccggcac gctgtcgagc
     9541 atcactttgc cggcctgttg cgcctgattc actacgttgg tcagcagatt ttccagctca
     9601 ttattcagcg tatacaccgt cagctcctgc ttgcgaacga acggatgagt aatgctgcgc
     9661 cgcagcgcca ggcgcacatc ggccgccagc agaatatgat ctttagtcac cgtgctactg
     9721 gcgaccagca cggtggcgat ggtgacgata tcgcgcaggg aaacgccttc ggtcagcagc
     9781 gcccggtaca ctttcagcaa ttggctgtaa ttgagcgccg cgcttaaatc ctccgccagg
     9841 cgcggtgcca tcgacgacaa acggttatgc aactgcgtga tgtcatcata gttaaacaaa
     9901 tcaggaatat agctgcgcac aatcttgttc acatgggtgg caatcacact ggcgctgtcg
     9961 atcacctgat accccatatt cagagccttc gctttctgcg caggctggat ccaggtgacc
    10021 ggtatcccgt acgccgggtc gttacccagc acgccgtcaa tctcgccgta ggtttcgctg
    10081 gagggcagcg ccatcagttt atccgccgga atatccgctt catcagcctt aatgccgttg
    10141 atgaaaatag cgtactggct gggcttgagg cggaagtttt cccgaatgcg gatctccggc
    10201 agcagcacgc cgttaccgtc ggaaatcacc tgccgaacgc cgcgaatccg ctgggtgagc
    10261 gggttaccct gcgctttgtc caccagcgcc accagcttgt agccgaggct taagctgatg
    10321 ggctcgatca gcggaatagt ttcccaactg acctgttgtt cgctggtttc agtgatggtg
    10381 cgggtcagcg tttcgaggct tttctcttcc gcctccgccg cctgcgggcg tttgctcatc
    10441 cgccagccgg taaagccaag cagggcgctg aacagcaaaa acggcagatg cggcatcccc
    10501 ggcaccaccg ccagcacgaa catgatcccg gtggcggtgt agagcatcga cgggcttgcc
    10561 agcaactggt ggcgcacgtc atgggcgata tcgccgttgt cgctgacgcg ggtgacgata
    10621 atcgccgccg cggtggagag cagcagggaa gggatctgcg ccaccaggcc gtcgccgatg
    10681 gtcatcagca cgtattgctg gaaggcggcg tcggcgctca ggttgtattt gaagatgccg
    10741 atacagacgc cgccgctcag gttgatcgcc agaatcatca tcccggcgat ggcgtctccg
    10801 cgtacaaact tcgatgcccc gtccatcgcg ccgtagaaat cggcctcgct ggcaacatct
    10861 ttacgccggg tttgcgcctg cgcctggttg atcaatccgg cgttaagatc ggcgtcaatc
    10921 gccatctgtt tgccgggcat cgcgtccagg gtaaagcggg cagaaacctc ggaaatacgc
    10981 tcggcctctt tggtgacgac gataaagttg attgtcaaca gcgtcagtaa atccgagcgt
    11041 gaaagcatta tcgccgcgct gcacgggcag tcgattttta acggtggtgg gctgtcgccg
    11101 ttaaataaaa tcagcccgtc tcatccgcca aaacctgcca ccgtggcggt gccggaagag
    11161 acggagaaaa aagcgcgtga tgtgaatgag aaaacggcgc tgctgaagaa gaaatccgcc
    11221 accgagcttg gcgagttggc aaccagtatc aacaccattg cccgtgatgc gcatatggaa
    11281 gcgaatctgg agatggagat tgttccccag ggattacgcg tgctgattaa agacgaccag
    11341 aatcgcaata tgttcgaacg cggcagcgcg cagattatgc cgttctttaa aactttgctg
    11401 gtggagctcg cgccagtgtt cgactcgctc tataacaaaa ttattattac cgggcatacc
    11461 gatgcgatgg cctacaaaaa caatatctac aacaactgga acctttcggg cgatcgcgcg
    11521 ctttcagctc gtcgggtgct ggaagaggcc ggaatgccgg aagataaagt gatgcaggta
    11581 agcgcaatgg cggaccagat gctgctggac gcgaagaatc cgcaaagcgc gggcaatcgg
    11641 cgtattgaga ttatggtact gaccaaaagt gcgtccgata cgctgtatca atactttggt
    11701 cagcatgggg ataaagtggt gcagccgctg gtgcaaaagc tggataagca gcaggtgctt
    11761 tcgcagcgaa tgcgctaaat gctgaatctt tacgcatttc tcaaaacctg aaatcactgt
    11821 atactttacc agtgttgaga ggtgagcaat gcgtaaaatc attcatgtgg atatggactg
    11881 ctttttcgca gcggtggaga tgcgcgacaa tcccgctctg cgcgatatcc ctattgctat
    11941 tggcggcagc cgcgaacgtc ggggggtgat cagcaccgcc aattatcccg cgcgtaaatt
    12001 tggcgtacgt agcgctatgc ctactgggat ggcgctcaaa ttatgcccac atctcacctt
    12061 gcttccgggg cgctttgacg cctacaaaga agcctcaaat catatccgtg aaatcttctc
    12121 gcgctacacc tcgcgcattg aaccgttgtc actggatgag gcttatctcg atgtcaccga
    12181 tagcgtccat tgccacggtt ctgcgaccct catcgcccag gaaatccgcc agacgatctt
    12241 caacgagctg caactgacgg catctgcggg cgtggcacca gtaaagtttc tcgccaaaat
    12301 cgcctccgac atgaataaac ccaacggtca gtttgtgatt acgccggcag aagttccggc
    12361 atttttacaa accttaccac tggaaaaaat ccccggcgtc ggcaaagtct cggcggcaaa
    12421 actggaagcg atggggctgc ggacctgcgg tgatgtacaa aagtgtgatc tggtgattct
    12481 gcttaaacgc tttggcaaat ttggccgcat tttgtgggag cgtagtcagg ggattgacga
    12541 gcgcgacgtt aacagcgaac ggttacgaaa atccgtcggc gtggaacgca cgatggcgga
    12601 agatattcat cactggtctg aatgtgaagc gattatcgag cggctgtatc cggaacttga
    12661 acgccgtctg gcaaaggtaa aacctgattt actgattgct cgccaggggg tgaaattaaa
    12721 gttcgacgat tttcagcaga ccacccagga gcacgtctgg ccgcggctga ataaagctga
    12781 cttaatcgcc accgcgcgta aaacctggga tgaacgccgc ggcgggcgcg gtgtgcgtct
    12841 ggtggggctg catgtgacgt tgcttgaccc gcaaatggaa agacaactgg tgctgggatt
    12901 atgatgtata ctattatgta tattctggtg tgcattatta tgagggtatc actgtatgca
    12961 tcggattctc gctgaaaaat cggtcaatat cactgagtta cgtaaaaacc cagctaaata
    13021 ctttattgat caaccagttg cggttctttc taataatcgc cccgcaggat atctcttaag
    13081 tgccagcgca ttcgaagcgt taatggacat gcttgctgaa caagaggaga aaaagcccat
    13141 aaaggcgcgc tttcgtccaa gtgctgcaag attagaggaa attacacgcc gcgctaaaca
    13201 atatcttaat gatatgacgg atgatgattt caatgacttt aaggaataag gatgcgggta
    13261 ttcaaaacaa aacttattcg cctgcaactt acagcagagg aacttgatgc gttaacggcg
    13321 gattttattt cctataagcg tgacggtgtt ttgccagata tatttggtcg cgatgcactc
    13381 tacgacgact cgtttacctg gccattaatc aaatttgagc gagttgctca tattcatctg
    13441 gcaaatgtga ataatccatt tccgccacag ttgcgccaat tcagcagaac gaatgacgaa
    13501 gcgcatttgg tatattgtca gggtgcgttt gatgagcaag catggttgct cattgccatt
    13561 ctgaagcctg aacctcataa actggctcga gataacaacc aaatgcataa aattggaaaa
    13621 atggcagaag cgtttcgcat
//
LOCUS       DADMFL010000105        13605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.106, whole genome shotgun sequence.
ACCESSION   DADMFL010000105 DADMFL010000000
VERSION     DADMFL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.106"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..1181)
                     /locus_tag="JNZ27_003137"
                     /pseudo
     CDS             complement(1..1181)
                     /locus_tag="JNZ27_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1391..1834)
                     /locus_tag="JNZ27_003138"
     CDS             complement(1391..1834)
                     /locus_tag="JNZ27_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665373.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2349..3191
                     /locus_tag="JNZ27_003139"
     CDS             2349..3191
                     /locus_tag="JNZ27_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665374.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3298..3561
                     /locus_tag="JNZ27_003140"
                     /pseudo
     CDS             3298..3561
                     /locus_tag="JNZ27_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4188..4733
                     /gene="cobU"
                     /locus_tag="JNZ27_003141"
     CDS             4188..4733
                     /gene="cobU"
                     /locus_tag="JNZ27_003141"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY8665375.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4730..5473
                     /gene="cobS"
                     /locus_tag="JNZ27_003142"
     CDS             4730..5473
                     /gene="cobS"
                     /locus_tag="JNZ27_003142"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY8665376.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5485..6564
                     /gene="cobT"
                     /locus_tag="JNZ27_003143"
     CDS             5485..6564
                     /gene="cobT"
                     /locus_tag="JNZ27_003143"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY8665377.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6626..7561
                     /gene="ldtA"
                     /locus_tag="JNZ27_003144"
     CDS             6626..7561
                     /gene="ldtA"
                     /locus_tag="JNZ27_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8665378.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7617..7692)
                     /locus_tag="JNZ27_003145"
     tRNA            complement(7617..7692)
                     /locus_tag="JNZ27_003145"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7657..7659),aa:Asn,seq:gtt)
     gene            8019..8936
                     /gene="nac"
                     /locus_tag="JNZ27_003146"
     CDS             8019..8936
                     /gene="nac"
                     /locus_tag="JNZ27_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY8665379.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            9038..9988
                     /gene="cbl"
                     /locus_tag="JNZ27_003147"
     CDS             9038..9988
                     /gene="cbl"
                     /locus_tag="JNZ27_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY8665380.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(10026..10101)
                     /locus_tag="JNZ27_003148"
     tRNA            complement(10026..10101)
                     /locus_tag="JNZ27_003148"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10066..10068),aa:Asn,seq:gtt)
     gene            10106..11733
                     /gene="yeeO"
                     /locus_tag="JNZ27_003149"
                     /pseudo
     CDS             10106..11733
                     /gene="yeeO"
                     /locus_tag="JNZ27_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            11834..11909
                     /locus_tag="JNZ27_003150"
     tRNA            11834..11909
                     /locus_tag="JNZ27_003150"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11867..11869,aa:Asn,seq:gtt)
     gene            complement(12365..13081)
                     /locus_tag="JNZ27_003151"
     CDS             complement(12365..13081)
                     /locus_tag="JNZ27_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8665381.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(13424..>13605)
                     /locus_tag="JNZ27_003152"
     CDS             complement(13424..>13605)
                     /locus_tag="JNZ27_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8665382.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
BASE COUNT         3475 a         2944 c         3371 g         3815 t
ORIGIN      
        1 tcccacaggt ctgtcgcccc ttcagttgcc agttcgctgt ctttacagac gtaatctttt
       61 ttcatgcgac cgaaggcttc cctgacaaaa tcatcgacct aggcttcatc aacaggaagg
      121 gttccgtttt tccgtaactc atgaagcgca cggttagcca ggtatgcctg gcgtgaggat
      181 ccgggataaa cgcgggaggc gggcagcaca tcgctgttca gcattgccgg taccttctga
      241 cagactgcat cagccccatc gccattacgg tctttttcat caaatttatt gaccagcaca
      301 aacaggcggc cgactgacac atcagcaatg gcattaagct ctttacgaac ctcctcatcg
      361 gctttcgagt tcatctgggt gtagtccatc accgccagca ccgcagaggc tttctgcagt
      421 tgatcccgca tcatcacttc catctgcggt tgtccggctt cattagggcc gggggtatcc
      481 accagtgtga gtgttccctg gcattcatcc atccccacca gatggctgaa ttccacgtcg
      541 atcaccggca gtttctgcac ttctgcgtat tcatcaaaag gaaattcact ccccattgcc
      601 gcagcaagac gaaccagatc gtttaacgat gccagtccgg taaagattcc ctcctccccg
      661 tggtattcat ttttgagcca gccatcatcc gtcagaatat ccagcagttc gcgggtatcc
      721 ccggtttgct gcagggtctg tgcgacctgc tgtcctgccg gggtggcgac tttttcctgc
      781 agtgtgatta ataaattgcg gacaggctga atatgttcca gatggagaac cggctcagtt
      841 tttccgggaa cgtggcggat gagcgtcggt acagaggtca tggggcggtt acggttaggc
      901 agaatttcct gccccacaat ggcgttgatg gtggttgatt tgcctgcttt catcgtgccg
      961 acaacagcca gcaccatctc cctgcgggcg gttttgattt gttcgccctc cagctcctta
     1021 attcttttca gtgctttctg tttatcaaaa agctgcccgt tttcgttctt accgtcagat
     1081 aacacatccg gctcatcaac catttgccgg agcagattga tgctcagttg caaaagccgg
     1141 tcggcttcat cacaaagcag ggtgatgttc ttttcgtgca ttatttaacc ttcctgagac
     1201 gcattttcat gacaaccacg gaggaggcca tttactacaa gcgatcgttt ataacgatca
     1261 atataatata aacgatcgtc atattctatt aatatgaaaa acacaacgca agggggacaa
     1321 cccgggtatg gctgcgtttg cattgtgttt tcatagagtt aaaaatctgt aacgacaacc
     1381 cggcacatca ctaccgggtt aagaagaaaa tccagttcca taccgcgcgg gccacagaga
     1441 ccacggtgtc acgaacggcg cgcagggccg tacggacaac agaggcaaca cagacactct
     1501 ccgccgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggtc tgagtgcgga
     1561 cagactccgt gcgcagttcg tcctgcaggc gtgtcatcag agggatggcg gcatgtccgg
     1621 gaagcactgt catgagtgca ctgaccgggg tgacctcccc ctgaaacagt gcattacaga
     1681 gtgaggattt accggccccg cttttaccca taatgccagg cacgggttcg tgacggggga
     1741 gtttgcgcag atgttccagg atgtgacggg aaatcgggta aggcagggag gagagcggtt
     1801 tttcaattgc ctcaatggca tcagacggat tcatacatgt attcccagtg aaaaaagaca
     1861 aaaccccgtg caccggaaag gtggcggggg attcacggag ataatatctt tctgaaatta
     1921 ttttgaatgc aggtatatgt ggctgatttg tatcagctaa tgagtacagg tctgaatcta
     1981 atctgacagt ccgctttgtg ccaggagcgg actgacctgc tccccgatga ttaatacaca
     2041 gctatgctag taatgtctgt agatcgctgt gtgttgcatc tatcggttga actcacagtc
     2101 caaaccagac tgtcagcttt gattagattc tacctactca acctgtcaga taaggtctga
     2161 gctaatacat ctaatatatg gctttgtgca ggggacggct atcgcgaacg ttaggctagg
     2221 tatctgaata cgcacagttc acttggttgt acttcttgca ctgatctgct atcccgcaca
     2281 gctatttata ggtaatcttc agtgtataat ctgccgactt atggaagctt gtacaacagg
     2341 agaaatatat gaaaaaagaa ttgtttggac gttgcccgtt tgatctcgac gaagatgtaa
     2401 aaaaaatagc cgcgataaat aaatatatcc atgcaaaatt tgaggagcat aaaaataaaa
     2461 tagacaactt aaagggtgtg gaacaaaaat tgatggtctt acagtacttt aatgtactga
     2521 atgaacttag taatcaggct atctcttctt tgaatatagg agccttttca gcatcggaag
     2581 tattagctag ggttataata gaacaggctg ccaatcaatt ttatatagca atagatgatg
     2641 gcgaaaatgc acaagcatta ctcaaggcta gtaagaaatt agttcatagt aatggaaaac
     2701 ggtggctgga gtgtttaaaa tccaaaggaa tgactaatcc agcagcaaat gaaaggattc
     2761 gcatgggaaa agaactaact gatctattta atagattgtg gccaaataca cctgaatatc
     2821 caggaaccag aaaacttttt gaattaattg gctgggaaac acactatcat gcccattacg
     2881 ttcccctttg tgattcaata catacattct ctgatgatat ggtaaatata gtatctctat
     2941 ataatgcaat ccaaagtgat gaaactactg ctatcgaatt cattcttgca gttaaacaag
     3001 aaaataaacg gctggcaatt tataattttg taattgctac ggcccttcgg tgtgaggcct
     3061 tagtaaatgt atttaactct ttaggttatc aggatattat aacagaaatg acaccaacaa
     3121 tagatacagt caaccagact ataattcgat atgatgattt tgatcattcc agaattgatg
     3181 attgtcaata attaattgac agaatacgta catgcgcaac aactagtgat agttgactta
     3241 ttttcgctct tgagagacaa ccatactcaa atctcccaca ttgcaggaga tttgaatatg
     3301 aaaacgtcac cgtggaataa agaccgtacc atcggtcaaa aaaggccact tcagatatct
     3361 catatctggg ggatccggat tagacttgag ttggaaggta aaacacgcga tctagctctg
     3421 ttcaacatgg ctttggacag taagctacgg ggctgtgatt tggtcaaact caaagtatca
     3481 gatgctgcat atggcagttc tgtctcaagc agagcaacgg tgttgcaaca gaaaaccggc
     3541 agccctgtcc aatttgagat gcctttaata acacatcttt taaatgttga agaattatcc
     3601 cgcctgctga agaacgttgc tctgagtgtg ataaaccttt ttattcttga tgatgtcagc
     3661 gagaagatgg aatatatccc cgactgggtc gtggttaccc ggttaaacat ctggtggcgc
     3721 taataaatct ggcaagtcac attctgttga atgctgtgac agtgccttac cgagagagcg
     3781 aaattgtggt gttctagtgc atagtcgcca ccattccgga taattctatt gcgttgtttt
     3841 acaagttctt ttacagcgca gatctgtcgc tgacgttgaa tcaatagcct ggatttcaga
     3901 acacaatgcc cgtttgctgg ttggtccggc agtagcatta attcattatc tgaatgaaat
     3961 gtgtcaatcc acagaaaccg ctgtcaatga cgcattttgt cggacgttaa cacgagaaag
     4021 tctggctatt ccgtaatgcc atcttatttc gtaatggatt gagaatgctg gatttttaat
     4081 tatattacgc gcagtattaa tcgcgtaaat gttttgcggt cagcaacggt gggggatcca
     4141 ttgctctgtt caatgaatac catcatacat ttgaatcagg agtcggtatg atgattttgg
     4201 tgacgggagg cgcatggagc gggaagagtc gccatgcaga ggtgcttatt ggggactctt
     4261 cacaggttct gtatatcgct acctcgcaaa tccttgatga tgagatggct gcacggatag
     4321 aacatcatcg gcaaagccgc ccggagcact ggcgcacagt ggagcgctgg caacatcttg
     4381 atgaattaat tcatgcagac attaacccga atgaggctgt gttgctggaa tgcgttacca
     4441 caatggtgac taatctgttg tttgattatg gcggcgataa agaccctgat gaatgggatt
     4501 atcaggcgat ggaacaggcg attaatgctg agattcagtc gttgattgct gcctgccaac
     4561 gttgccccgc aaaggttgta ttagtgacta acgaagtggg aatggggatt gtgccggaga
     4621 gtcgtctggc acgacatttt cgtgatattg ccgggcgggt aaatcagcag ttggccgctg
     4681 cggcaaatga agtatggctg gtggtttcgg gtattggagt aaaaatcaaa tgagcaaatt
     4741 attttgggcg atgctctcat ttattacgcg tctgcctgta ccgcgtcgct ggtcccaggg
     4801 actggatttc gagcattatt ctcgcggtat tattactttt cctttgattg gattattgct
     4861 tggcgcgatt agcgggctgg tcttcatggt gctgcaggca tggtgtggcg caccactggc
     4921 ggcactgttt agcgtacttg tgctggcgct gatgaccggg gaattccatc tggacggcct
     4981 cgctgatacc tgtgatggcg tattttctgc acgtagctgc gatcgcatgt tggaaatcat
     5041 gcgtgatagc cgtttaggca cccacggcgg tctggcattg atttttgtgg tactggcaaa
     5101 gattctggtg ttaagcgagt tggctctgcg tggcgaaccg atccttgcat cgctggcggc
     5161 agcatgtgcg gtcagccgcg ggactgccgc attattgatg tatcgtcatc gttatgcgcg
     5221 ggaagaaggg cttggcaatg tgttcattgg caaaattgat gggcgacaaa cctgcgtcac
     5281 actcggctta gccgctattt ttgctgccgt attattgctg ggtatgcatg gtgtggctgc
     5341 tatggtggtg acgatggtgg cgattttcat cctcggtcag cttctcaaac gtacgctggg
     5401 cgggcaaacg ggcgatacgc tgggagcagc catagagctt ggtgaactgg tcttcctgct
     5461 ggcactgcta taaagagaca tgacatgcaa ataattgccg atttactgaa tacgatccca
     5521 gccatcaatt ctgccgctat gtcgcgtgca caacggcatg ttgacgggtt actcaaacct
     5581 gttggtagcc taggaaagct ggaggcgctt gccatacaac tggcaggaat gccgggggtg
     5641 aatggcatac cgcatgtggg caaaaaagcg gtactggtta tgtgtgccga tcacggcgtc
     5701 tgggaggaag gggtcgctat ttctccaaaa gaagtgacag ccatacaggc tgaaaatatg
     5761 acccgtggaa caaccggcgt gtgtgtgctg gcagcacaag cgggcgctaa cgtccacgta
     5821 attgatgttg gtattgatac tgctgagcct atccccgggc ttatcaacat gcgtgtcgca
     5881 cgaggtagcg gcaatattgc ttcagctccg gcaatgagtc gccgtcaggc tgaaaagttg
     5941 cttttggacg tcatatgtta tacgcgggag ctggcgaaaa acggtgtcac gctgtttggt
     6001 gtaggtgaac tggggatggc aaacacgaca ccggcagcgg caatagtcag cacaatcact
     6061 ggccgggatc ctgaagaagt ggttgggatt ggcgcaaacc tgccgacaga taaactggct
     6121 aataaaattg atgttgtgcg tcgggcgatt acgttgaatc aaccaaatcc tcaggatggt
     6181 gttgatgtcc tggcaaaagt gggtggattt gatttggtcg gaatagctgg agtgatgtta
     6241 ggtgctgctt cctgcggttt acccgtgttg ctggatggat ttctttctta tgctgctgcg
     6301 ctcgcagcct gccagatgtc tcctgcaatc aaaccgtatc tcattccttc tcacttgtcg
     6361 gcagaaaaag gcgcgcgtat agcgctctcg catttggggc tggagcctta tctcaatatg
     6421 gatatgcgtt taggtgaggg gagtggtgca gctctggcga tgtccatcat cgaagctgct
     6481 tgtgcgatat acaacaacat gggcgaactt gctgccagta atattgttct accggggaat
     6541 acgacttctg atttgaacag ctaaaaacga aaagtctgat aaaagctata cttatcggat
     6601 agtattcgcg acttaggaaa gggacatgat gcgtcgtgta aatattcttt gctcatttgc
     6661 tctgcttttt gccagccaaa atagcctggc ggtaacttat ccattacctc cagagggtag
     6721 ccgtttagtg gggcagtcgc ttactgtaac tgttcctgat cacaatactc agccgctgga
     6781 gacttttgcc tcacaatacg ggcaagggtt aagtaatatg ctggaagcga atccgggcgc
     6841 tgatgttttt ttaccgaagc ctggctcaca actcaccatt ccgcagcaac tgattttgcc
     6901 cgccactgtt cgtaaaggga ttgtggttaa cgtcgctgaa atgcgtcttt attactatcc
     6961 gccagacagt aatacagtgg aagtctttcc tattggtatc ggccaggctg ggcgagaaac
     7021 cccgcgtaac tgggtgacta ccgttgaacg taaacaagaa gcaccaacct ggacgccaac
     7081 gccgaacacc cggcgcgaat atgcgaaacg aggggagagt ttgcccgcat ttgttcctgc
     7141 ggggcccgat aaccccatgg ggctgtacgc gatttatatt ggcaggttgt atgccatcca
     7201 cggtaccaat gccaattttg gtattgggct ccgtgtaagt cagggctgta ttcgtctgcg
     7261 taatgacgat atcaaatatc tgtttgataa tgttcctgta ggcactcgtg tacaaattat
     7321 tgatcagcca gtgaaataca caacggaacc ggatggttca aagtggctgg aagttcatga
     7381 gccgctgtcg cgcaatcgtg cagaatatga gtctgaccga aaagtgccat tgccggtaac
     7441 cccatctttg cgggcgttta tcaacgggca agaagttgat gtaaatcgcg caaatgctgc
     7501 gttgcaacat cgatcgggaa tgcctgtgca aattagttct ggttcaagac agatgtttta
     7561 agagcgttgg taattagaaa gtaaaaagcc tgctcgaaag caggcttttt tgaatttggc
     7621 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
     7681 aactacagag gaatcgtgtg aacggggcgc atattaatgg cagtgcatgg tgatgtcaaa
     7741 gggtgaaatg catttttttg tttgtttgct gccaatttca acaaagtggt gagctttcaa
     7801 tcttattggg taatgaacca tcgtggtgca taccctcctt ttatagggca ggtgaatgcg
     7861 actgctgata aaaagggcca atgacgaatc tatcataatc tcttatttga cagttggtta
     7921 gcttgcgcat caacgccaaa atagaactgg caggcatctt gcatactggt tgtaagtaat
     7981 ggcggcactt gggccgattc ttaaaaaccg gaggcaacat gaacttcaga cgcctgaaat
     8041 acttcgtaaa aattgtagat attggtagcc tgacccaggc tgctgaagta ttgcatatcg
     8101 cacaaccagc gctcagccag caggttgcca tactggaagg tgagttaaat caacagcttt
     8161 tgattcgcac aaaacggggc gttacgccaa cagacgccgg aaaaattctc tatacccatg
     8221 cacgggccat tttacgtcag tgtgagcagg cccaactggc ggtgcataac gttggtcagt
     8281 cattatcggg gcaagtctcg attggctttg cgccaggaac cgctgcgtca tccatcacca
     8341 tgcccttatt acaggcggtt cgcgctgaat tcccggaggt cgttatctat cttcatgaaa
     8401 atagtggcgc agtgcttaac gaaaaactga taaatcacca gctcgatatg gcggtgattt
     8461 atgagcattc ccctgtggct ggggtatcca gtcaggcttt gctgaaagaa gatcttttcc
     8521 tggtaggaac tcaagattgc ccggggcaaa acgttgatgt taatgctatt gcgcaaatga
     8581 acctttttct ccccagtgat tacagcgctg ttagacttcg tgttgatgag gctttttccc
     8641 tacggcggct cacggcaaaa gtcattggtg aaattgagtc tattgccacg cttaccgcag
     8701 cgattgccag cggcatgggc gttgcagtat tacctgaatc ggctgcgcgt tcgttatgtg
     8761 gcgcagtaaa tgggtggatg tcacgcatta ccactccttc aatgagtctc tctttgtcct
     8821 taaatttacc cgccagagca aacttgtcac cacaggcaca ggcagtgaaa gagttgttaa
     8881 tgtcagtgat tagttctcca gtgatggaaa aaaggcagtg gcaattggtg agctaagcgt
     8941 tatatcggga tggaataaga tgcgggtttt tattatttgt tatgccgggc attagacttt
     9001 aacaataacg ggatatctga actgcccgga gtttaccgtg aatttccaac aactaaagat
     9061 aatccgcgag gctgcacgtc aggattacaa cctgacagag gttgcgaata tgctttttac
     9121 ctcacagtca ggcgttagcc gtcatattcg ggaactggag gatgaacttg gcatcgaaat
     9181 atttgttcga cgaggtaagc gactgctggg gatgactgaa ccgggcaaag cattactggt
     9241 cattgcagaa cgtattctga atgaagccag taatgttcgt cggcttgcag acctgtttac
     9301 caacgatacg tctggtgttc tcactattgc aacgacgcat actcaggcac gttatagctt
     9361 gccagaggtc attaaagctt ttcgcgaact tttcccggag gttcggctcg agctaatcca
     9421 gggttcgcca caggaaattg cgacgttgtt gcaaaatggc gaagctgata ttggtatcgc
     9481 cagcgagcgt ttgagtaatg acccgcagct cgtcgccttc ccgtggtttt gttggcacca
     9541 tagtttgctt gttccactcg atcatccctt aacgcaaatt acaccattga cgctggaatc
     9601 aatagcgaag tggccgttaa ttacttaccg acagggaatt acggggcgct cacgcactga
     9661 tgacgcattt acccgcaaag gtttgccggc agatattgta ttaagtgcac aggattctga
     9721 tgtcattaaa acctatgttg ctcttgggct tgggatcgga ttagttgccg aacaatccag
     9781 cggcgaacaa gaggaagaga atttaatccg cctggatacg cggcatcttt tcgatgctaa
     9841 tactgtctgg ttgggactga agcgaggaca acttcagcgt aactatgttt ggcgctttct
     9901 ggaactttgt aatgcaggac tgtcagttga ggatatcaag cgccaggtga tggaaaacag
     9961 tgaagaggaa attgattatc agatatagca aaaagcccgc tataaaagcg ggcttttcag
    10021 aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc
    10081 taccgactga actacagagg aatcgctgtt gaggcacatc ttaacggcga aaaatatttt
    10141 gtcaaacccg atttttaaat ttcccaactg tttgccgttt ctatcaacag tttgttgcat
    10201 ttgcagacaa tttgttggcg aaaatctttg cagctttgct gattctccct catcatttga
    10261 aatgtggttt cactttctgc aattaaggtc ggctttgaat atctcctctg ctttacgcca
    10321 ggttgttcac ggcactcgct ggcactctaa acgcaagagc tacaaagtgt tgttctggcg
    10381 cgagataacc ccgcttgctg ttcctatctt catggagaac gcctgtgtcc tgttgatggg
    10441 ggtcctgagc acttttctgg tcagctggct gggaaaagat gcgatggccg gcgtggggtt
    10501 ggcggacagc ttcaatatgg tcattatggc tttttttgca gctatcgatc ttggtactac
    10561 tgtcgttgta gcatttagtc tcggtaagcg ggatcgacga cgagcgaggg tggcgacgcg
    10621 gcagtcattg gtgatcatga cgttgtttgc cgtactgttg gcaacgctaa ttcatcattt
    10681 tggcgaacaa attattgatt tcgtcgcggg tgatgccacg acagaagtta aagcactggc
    10741 gttgacttat ctggagctga ccgtactcag ttatccagcc gctgccatca ctcttattgg
    10801 tagcggggca cttcgtggtg cagggaatac gaaaataccg ctactgatta acggtagcct
    10861 gaatattctt aatattatta ttagcggcat attgatttac ggccttttct cctggtcggg
    10921 gctgggttgg gtttaaccat ttctcgttat attggcgcag ttgcaatttt gtgggtgctg
    10981 gcgattggtt ttaatcctgc gctaaggatt tcgttaaaga gctattttaa accgctgaat
    11041 tttagcatta tctgggaagt catggggatt ggtattccag cgagtgtcga atcagtgtta
    11101 tttaccagtg gtcggttatt aacccaaatg ttcgttgccg ggatggggac cagcgttatt
    11161 gccggaaatt ttatcgcgtt ctcaattgcg gctcttatca acttacccgg aagtgcgctc
    11221 ggttctgctt ctacgatcat tacaggccga aggttggggg tagggcagat agcgcaagca
    11281 gagattcagt tgcggcatgt gttctggctg tccactcttg gattaacggc catcgcctgg
    11341 ctaacggctc cctttgccgg ggttatggca tcgttttaca cccaggagcc acaggttaaa
    11401 cacgtcgttg tgattctgat ttagctaaat gctttattta tgcctatttg gtccgcctca
    11461 tgggtgctac ccgctggatt taagggtgct cgtgatgccc gttacgccat gtgggtttct
    11521 atgttgagca tgtggggttg ccgggttgta gtcggttatg tgcagggaat catgcttggc
    11581 tggggtgtgg ttggtgtctg gatgggaatg tttgcctact gggctgtgcg ggccgtgctg
    11641 ttttactggc gaatggttac tggacgttgg ctgtggaaat accctcgatc cgagccgcaa
    11701 aagtgtgaaa aaaagccagt tgtgtcggaa taaacgacaa aatgcagatt atttcagcaa
    11761 acgatttcaa atttaaaaaa caggctttga cattgtgggt gggcatcgct aatattcgcc
    11821 tcgttctcac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
    11881 cactggttcg agtccagtca gaggagccaa attttagttt tgagacatct ctatgagact
    11941 caggacagtt aaaaaataaa aattattttt acccggccac tttgtaaaaa agtggccggg
    12001 ttttttgatt tctgtgtttc gtgcgcttaa tcgagtctca cgcttcagtg agcaaattgc
    12061 ccctccccaa cagacatcac gattcacaag gtcaaaaatt ctgttccatg aagtaccgta
    12121 caaagataaa gagaaaaatg ccttatgcgg gatgtagctg tcgcgtcttt atcgggaaac
    12181 tatacgtaat tgccactttg tcttcggtaa tcagaaaaag aagacctgcc attttaataa
    12241 atattatttc tctgggaacg gggatttttt ataagcaata tgacgagtta aacattatat
    12301 tttttataag aattatatga gattaaagaa atctgccata aagacagatt tctttaaaat
    12361 ataattagag atttgcgacg ttatgataaa ctttttgtac atcgtcgtca tcttcaaggg
    12421 catcaacaag cccttcaaag atttctaaat cttctgggga aagctcaact tcagattgag
    12481 caatcatttc taattctgtt gttgagaact cagtgattcc agctgctttt agcgccgcga
    12541 ttcctttatg aaggtcagca ggttcagtat aaataacaat attaccttct tcttcagtta
    12601 catcacgaac atcaacttca gcttcaagca aaatttcaaa aatatggtca gggtctgttc
    12661 ctttaaatac aatcacaccc gtattgtcaa acatatagct gacagaacct gccgctccga
    12721 tattgccgcc ttttttattg aaaattgtgc gaacgttagc aatcgtacgg ttaacatttg
    12781 aagtcaatgt ctcagcaata atcattgagc cattaggacc aaagccttca taacgtccct
    12841 gtacgaacgt ttcatctccg ccgcctttgg ctttatcaat tgctttatca ataacgtgct
    12901 ttggaacttg tgcctgcttt gcacgttcaa taacgaattt taaagatgtg tttaattctg
    12961 gatcgggttc accttgtttg gcagcagcat agatttctac accgaatttt gcataaattt
    13021 tagacgttgc accgtcttta gccgtttttt tagcaacaat attggcccat ttacgtccca
    13081 ctggaatgat ctcctcgaaa atttatttac attactggta aatcgttaac atgtgttaat
    13141 tcggcatatt atatcataac aggaaaaatc tcgtctatta gaaataaaaa tattctaaag
    13201 ccataatctt atatttaatt ttataaaaac taatgttgtt ggcgggattt tttataattg
    13261 tgttttgaaa gcatacttgc ttttaatatg tggaaatcat taagacgata ataaaactcg
    13321 catctgccca aggaagcaag cagcccgcgt ttattgcagg ctatttgcca gatgtcttac
    13381 ttaatccttc acatctattt ctgataagaa ggttcataac ttattatcgg aacggcggct
    13441 cattaaaggt tcgtaattta cgtgaatgca gtcggtcgcc ttctgcgcgc agcaaatcga
    13501 tcgcccgaat gccaatttgc aggtgttcgg aaatagcgcc ttcataaaaa cggttagcct
    13561 ggccgggaag cttaatctca ccatgtaacg gtttatctga aacac
//
LOCUS       DADMFL010000106        13543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.107, whole genome shotgun sequence.
ACCESSION   DADMFL010000106 DADMFL010000000
VERSION     DADMFL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.107"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..892)
                     /locus_tag="JNZ27_003153"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="JNZ27_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1133..1933
                     /locus_tag="JNZ27_003154"
     CDS             1133..1933
                     /locus_tag="JNZ27_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8665383.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2011..2781
                     /locus_tag="JNZ27_003155"
     CDS             2011..2781
                     /locus_tag="JNZ27_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8665384.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAETRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="JNZ27_003156"
     CDS             complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="JNZ27_003156"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY8665385.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="JNZ27_003157"
     CDS             complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="JNZ27_003157"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY8665386.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5048..5770
                     /locus_tag="JNZ27_003158"
     CDS             5048..5770
                     /locus_tag="JNZ27_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8665387.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="JNZ27_003159"
     CDS             complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="JNZ27_003159"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY8665388.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6299..7030
                     /gene="dnaQ"
                     /locus_tag="JNZ27_003160"
     CDS             6299..7030
                     /gene="dnaQ"
                     /locus_tag="JNZ27_003160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY8665389.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7163..7239
                     /locus_tag="JNZ27_003161"
     tRNA            7163..7239
                     /locus_tag="JNZ27_003161"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Asp,seq:gtc)
     gene            complement(7370..8416)
                     /locus_tag="JNZ27_003162"
     CDS             complement(7370..8416)
                     /locus_tag="JNZ27_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY8665390.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8427..9302)
                     /gene="tssG"
                     /locus_tag="JNZ27_003163"
     CDS             complement(8427..9302)
                     /gene="tssG"
                     /locus_tag="JNZ27_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY8665391.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCREIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="JNZ27_003164"
     CDS             complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="JNZ27_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY8665392.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMLRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="JNZ27_003165"
     CDS             complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="JNZ27_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY8665393.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11809..12639)
                     /locus_tag="JNZ27_003166"
     CDS             complement(11809..12639)
                     /locus_tag="JNZ27_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY8665394.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12626..13003)
                     /gene="tagK"
                     /locus_tag="JNZ27_003167"
     CDS             complement(12626..13003)
                     /gene="tagK"
                     /locus_tag="JNZ27_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HAY8665395.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13240..13410)
                     /locus_tag="JNZ27_003168"
     CDS             complement(13240..13410)
                     /locus_tag="JNZ27_003168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HAY8665396.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3104 a         3523 c         3614 g         3302 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctgttgcaaa atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg
      961 atatgtcatc cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt
     1021 gcttacataa caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta
     1081 gaaccctccc gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa
     1141 aaacacctat gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg
     1201 gtcttttgcg agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg
     1261 tattcggatc ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt
     1321 aaagaactac ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga
     1381 gttagtacag tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct
     1441 gccacaacat ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc
     1501 gttacgtgaa cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc
     1561 attacctgac acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg
     1621 cgtggatgtc tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg
     1681 cccggtcatt atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata
     1741 tcgctttgcg cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg
     1801 ggcgtttggc cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc
     1861 tgtattggtt acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa
     1921 gcctgataaa taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct
     1981 tttatactca aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta
     2041 gaaaagcagt ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga
     2101 cgagatttac agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat
     2161 atgggctgcg gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg
     2221 gcgtatgact tatctgccca aatgctggat gtcgtggcac aagctgccga aacccggcaa
     2281 ctgaaaaata tcaccacccg ccagggatat gccgaaagtc tgccatttgc cgataacgca
     2341 tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg
     2401 cgtgaagtga accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct
     2461 ccgggtcatc cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct
     2521 cacgtacgaa actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata
     2581 gttgataatt taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg
     2641 cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag
     2701 gtgagaacgt attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta
     2761 gaagcacata aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat
     2821 ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct
     2881 ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg
     2941 cgtttagcaa tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg
     3001 ttgtttgcca gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc
     3061 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     3121 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     3181 gtattgtcgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     3241 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     3301 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     3361 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     3421 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3481 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3541 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3601 ttataagccg ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc
     3661 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3721 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3781 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3841 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3901 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3961 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     4021 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     4081 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     4141 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     4201 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     4261 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     4321 tttcttcatt aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     4381 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     4441 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4501 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4561 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4621 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4681 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4741 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa
     4801 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4861 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4921 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4981 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     5041 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     5101 tttgccctgg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     5161 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     5221 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     5281 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     5341 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     5401 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     5461 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5521 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5581 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5641 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5701 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5761 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5821 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5881 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5941 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     6001 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     6061 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     6121 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     6181 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     6241 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     6301 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     6361 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     6421 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6481 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6541 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6601 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6661 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6721 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6781 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6841 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6901 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6961 gctggcagca catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg
     7021 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     7081 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     7141 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     7201 cgcagggggt cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     7261 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     7321 ggatatgatt taaaaaatgg ccagacatta taaattctgg ccacttctgt tactccagcg
     7381 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     7441 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7501 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7561 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7621 gctctgcctg catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg
     7681 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7741 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7801 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7861 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7921 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7981 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     8041 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     8101 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     8161 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     8221 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     8281 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     8341 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     8401 acaggaatat ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc
     8461 atgcccgtca ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg
     8521 tacggaccac cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc
     8581 ctgcagtatt cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg
     8641 ctgccaggca gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac
     8701 ttatactgca tgtccggcac ggcctggccg atgagtgcac cattgccaat aacggaagag
     8761 ggggcaggca tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca
     8821 tactcctgta tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc
     8881 ggattgcggg actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac
     8941 gggctgtcag cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag
     9001 cggtcatgtt gcggtttatc cagacctccc ccagcggact gggcgctgcg ccatgcctgg
     9061 taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg
     9121 cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc
     9181 ggcccccaca gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca
     9241 ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc
     9301 atccgtggtt gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc
     9361 atcaggttca gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc
     9421 atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg cgcagcatgc
     9481 tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca
     9541 gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt
     9601 cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg
     9661 gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag
     9721 gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg
     9781 ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag
     9841 ccagcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg
     9901 ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt
     9961 tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact
    10021 ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc
    10081 gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc
    10141 cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg
    10201 tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt
    10261 ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg
    10321 gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga
    10381 tcagccagtc cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa
    10441 ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg
    10501 caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc
    10561 cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc
    10621 cgtcaaaatg ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta
    10681 acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtccgg
    10741 caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga
    10801 cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca
    10861 gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc
    10921 aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa
    10981 atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg
    11041 gcgtgggtgt gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga
    11101 attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc
    11161 gctctacata cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg
    11221 gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt
    11281 atcccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct
    11341 gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct
    11401 cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg
    11461 ggatgatttg cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa
    11521 tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca
    11581 ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt
    11641 catggctgac ggtgttgagc agaaatgaca aatcccggcg gatgatctcc ttaatctgaa
    11701 ccgggctgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt
    11761 caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct
    11821 tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc
    11881 ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca
    11941 gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg
    12001 aaacagccag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat
    12061 caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag
    12121 ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc
    12181 gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag
    12241 cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt
    12301 ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc
    12361 ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc
    12421 gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca
    12481 gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg
    12541 catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag
    12601 cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg
    12661 ccgggtttat ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat
    12721 ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga
    12781 tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta
    12841 cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt
    12901 cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa
    12961 aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcc ggtattcctg
    13021 ataaagtcgg gtcagggcat ttcctttttc cgcaccttct ccggtggagg aggctacatg
    13081 aacaggaatg atatcgaatg gattttgtga tgtcagatgc tgctgcctga accaatccag
    13141 atccagatat tcggttattg cttccgacgt gatggcagtc tgcgatatcg tttttactgt
    13201 gctcaggtcc ggagatgatg gattgccatg tacaaggcac tatgaagata acccccattc
    13261 tatcaggtcg ccttcattca gacgcatacg gtgatgaggt tccacaatct ggtcgtttac
    13321 cgtacagcaa tattcggtag actgattgat aatccaccat gcttcttcgt ggcgggtcag
    13381 agtgagggca atctttacat catcattcac cttaaaaaca ggaatccagg gagaggtaac
    13441 ggtgaagata atttgtgttc cttcagtcgc gtaaattgtg ttatcggcga tcccgtgcgc
    13501 ctgtattttt tggagtcccc acgtaatatt tggtttcatt gtg
//
LOCUS       DADMFL010000107        13470 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.108, whole genome shotgun sequence.
ACCESSION   DADMFL010000107 DADMFL010000000
VERSION     DADMFL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.108"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            49..579
                     /locus_tag="JNZ27_003169"
     CDS             49..579
                     /locus_tag="JNZ27_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8665397.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JNZ27_003170"
                     /pseudo
     CDS             597..1235
                     /locus_tag="JNZ27_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JNZ27_003171"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JNZ27_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY8665398.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADFNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JNZ27_003172"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JNZ27_003172"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY8665399.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JNZ27_003173"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JNZ27_003173"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY8665400.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JNZ27_003174"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JNZ27_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY8665401.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JNZ27_003175"
     CDS             complement(5552..6613)
                     /locus_tag="JNZ27_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY8665402.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JNZ27_003176"
     CDS             complement(6610..8007)
                     /locus_tag="JNZ27_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY8665403.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JNZ27_003177"
     CDS             8163..9161
                     /locus_tag="JNZ27_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY8665404.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JNZ27_003178"
     CDS             complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JNZ27_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY8665405.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10222..11304)
                     /locus_tag="JNZ27_003179"
     CDS             complement(10222..11304)
                     /locus_tag="JNZ27_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8665406.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(11318..11977)
                     /gene="pgmB"
                     /locus_tag="JNZ27_003180"
     CDS             complement(11318..11977)
                     /gene="pgmB"
                     /locus_tag="JNZ27_003180"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY8665407.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11974..13470)
                     /locus_tag="JNZ27_003181"
     CDS             complement(11974..13470)
                     /locus_tag="JNZ27_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAY8665408.1"
                     /translation="MCAQQSYDQHLAASTENWRQWWQKRRITVNGGDAHDQQALDYAL
                     YHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSEPTVARSLLRYRW
                     HNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLV
                     ADIDWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYT
                     EHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQ
                     DDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSSA
                     SCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDD
                     GIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDA
                     QRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE"
BASE COUNT         3334 a         3650 c         3219 g         3267 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag acattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagattt caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tcttttcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac cacgttccac gtttacccgc gctataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgttctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttcattat tgttccttgt attatcctag
    10201 gggaatgcct tccccctgta tttagcgaat tgctatttcc gtttctgcat caaagaaatg
    10261 acatttcgtc atatcaaaat gaatagtgat attttctcct gcatgataat catttaacgc
    10321 accagcacgg accactaact cgtgcccccc aaccgtggtg tagagcataa attccgcacc
    10381 ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg cgtccggatg
    10441 aatatcttcc gggcgtattc ccatcacgat gggcttatgc aaactttcct gtgttttcag
    10501 aaccgctaat ttctcttcga gaatggttaa tttaagcgtt tccgtaacga atttatcgcc
    10561 atcgatcgtg ccgcgaataa aattcatcgc tggtgatcca ataaatccgg caacaaacat
    10621 attcgcgggt tggttataaa cggttttcgg cgcacctact tgctgaacaa tcccgtcttt
    10681 cataatcaca atccgcgtcg ccatggtcat cgcttcggtc tgatcgtggg tcacgtagat
    10741 catggtggtg ttcagtttct gatgcagctt gctgatctct gcacgcattt gtacgcgcag
    10801 cttggcatca aggttagaga gcggttcatc cattaaaaac acgcccgctt cgcgcacaat
    10861 cgcccgcccg agcgccacac gctggcgttg cccgccggaa agcgcgcccg gcttgcgttt
    10921 caggtactca cgcaggccga gaatttgcgc cgcccagttc acccgctcat caatcacctc
    10981 tttggcgatt ttttgcatct tcaggccaaa cgccatgttg tcgtaaaccg tcatatgcgg
    11041 atacaacgcg tagttctgga acaccatcgc gatattgcgt gctttggctg gaacgtcatt
    11101 cattcgtttg ccgtcgatca acagatcgcc gccgctgatc tcctcaagcc cggcaatcat
    11161 gcgcagggtg gtcgatttac cgcagcccga cgggccgaca aatacgatga actctttatc
    11221 ggcaatttcc aggttgaagt ccttcaccac atgcacctgg ttatcgtaga ttttttgaat
    11281 atgttgtaac gaaagctgag ccatgttgat tcctttgcta tacgttttgc cagaaggccg
    11341 ataaccgcga ccaggtcagg gagtccgttg agggcaacag taattgcgcc ccggttaagc
    11401 ccgcaccgat ccccaccgag cgcataccgc tggcgttaat ggcgtcaatg cccgcctgcg
    11461 catcttcaat gccgatacat gcctgcggcg gcacgcccag cccggcacag gcagcgagaa
    11521 agatttccgg atccggtttc gagtttttaa gttgggaagc atccgcgcag aaggtgaaaa
    11581 actcgcgcag ctccagcgcc gctaaaatcg tcggcgcatt cagggagacg gaagccagcc
    11641 ctaccggaat ctgctgtgca cggagctctg ccagcaaatt gcgaataccg ggcagcacag
    11701 cattaaccgt cagctcgcgc agtgagtgga cgtaaagcag atttttgcga tacgccagtt
    11761 gcgcccgctc ctgcgggtta aagtcgccct ctttgccccc gtgttgcaga atgcgccgca
    11821 gagactcatc gcggctgatc ccttttagag attcgttaaa ctgcgcatca atactgatgc
    11881 caatttcagc ggcaatctgc tgccacgcct ggaaatgcag atgcgcggta tcggtgatta
    11941 caccatccag atcgaaaatt accccttgca gtttcattca tcctcctgat gcgtggtggc
    12001 ggtcccattg aagggcaaaa taaaatcacc caaacagaaa acagattctt cagccacgga
    12061 tataagctga ccgttcaaac gcagtgaaac gggcgcagaa gttcgaatcg caatacgctg
    12121 cgcgtcgaga gtgacctgta attcgcagcc ctgccagaac agagggaaag acaactgttg
    12181 ccactgctca ggtaacgccg gatttagatg caattcaccg tcacgcacgc tcaccccggc
    12241 aaaaccctga atcgccccca gccagatagc gccagttgcg gcagcatgga taccatcatc
    12301 acaactatgc ggatcagcac caagatcgat ttcagtcccc tcgcgccaga actgataact
    12361 ttgggtcagc aggccacagc gtgcagcaac aatgccgtgg attgctttac ttaatgacga
    12421 gtcgtgaata gtgcgcggtt cataaaattg cagattggca agacacgatg ccgatgagaa
    12481 ctgctctggc agcatgtaat tgagcatcac cacatcagct tgtttgagga tctgcatctc
    12541 gttcacttct gcgcgtgaat aatccagcag aatggtttgc ttccccgccg ccgctttgta
    12601 tttcgccaga ttaatcgccg gcttagtcat aaacgaatca tcctgcggca aaacaccgtc
    12661 gggctgaatt tctggcagcc gcagttcttt aaggaacatt tcggcacgat gaataaacgc
    12721 gtcgtcacta cagccaaact ggcgggcaat actcagcgcc tgctggacgt tgtaatacac
    12781 catatagctg gtgaaggcgt tattattgac atgttcggta tattcgtctg gcccaataac
    12841 atcatgaatt tccagacggt cgttaaccct caccgcgcgg ctaatccaga actttgcagt
    12901 ttccagaagt agcgccatgc cttcatgcgc aatgaaactt tcatcccccg tggtctgcca
    12961 gtattgaata accgcccagt cgatatcggc caccagatga tgttccgcct gcgccgaggc
    13021 cactttttgc cgcagcccgg tgcgaatgtt aatggcggca aattccggcg tctcttcttc
    13081 gccgctgcgc gcgctttccc acggaaatag cgcgccctgc cagccgttgc gtcgcgcttt
    13141 ctcctgcgcg cctggcaagt tgtgccagcg ataacgcagt aaacttcggg caaccgtcgg
    13201 ctcgctaaac agatggaacg gcagcaaaaa tacttctgta tcccagaaaa cgtggccttt
    13261 gtagccttcg ccggttaagc cttttgccgc aatactgctg cgctcgccgt gagccggcgt
    13321 catgatgcgc agatgataaa gcgcataatc taacgcttgc tgatcgtgcg catcgccgcc
    13381 atttaccgtg atacgacgtt tctgccacca ttgacgccag ttttctgttg atgctgcaag
    13441 atgttggtcg taactctgtt gcgcgcacat
//
LOCUS       DADMFL010000108        12795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.109, whole genome shotgun sequence.
ACCESSION   DADMFL010000108 DADMFL010000000
VERSION     DADMFL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.109"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JNZ27_003182"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JNZ27_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY8665409.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JNZ27_003183"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JNZ27_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY8665410.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JNZ27_003184"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JNZ27_003184"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY8665411.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3174
                     /gene="rutC"
                     /locus_tag="JNZ27_003185"
     CDS             2821..3174
                     /gene="rutC"
                     /locus_tag="JNZ27_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY8665412.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLAFDQHNNVLFADDPKAQTRHV
                     LETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKPARFYIQCGLVK
                     PDALVEIATIAHIAK"
     gene            3182..3982
                     /gene="rutD"
                     /locus_tag="JNZ27_003186"
     CDS             3182..3982
                     /gene="rutD"
                     /locus_tag="JNZ27_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY8665413.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            3992..4582
                     /locus_tag="JNZ27_003187"
     CDS             3992..4582
                     /locus_tag="JNZ27_003187"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY8665414.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLSPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4593..5087
                     /gene="rutF"
                     /locus_tag="JNZ27_003188"
     CDS             4593..5087
                     /gene="rutF"
                     /locus_tag="JNZ27_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY8665415.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5108..6436
                     /gene="rutG"
                     /locus_tag="JNZ27_003189"
     CDS             5108..6436
                     /gene="rutG"
                     /locus_tag="JNZ27_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY8665416.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6518..6625)
                     /locus_tag="JNZ27_003190"
     CDS             complement(6518..6625)
                     /locus_tag="JNZ27_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665417.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            6998..7594
                     /gene="wrbA"
                     /locus_tag="JNZ27_003191"
     CDS             6998..7594
                     /gene="wrbA"
                     /locus_tag="JNZ27_003191"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8665418.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7615..7842
                     /locus_tag="JNZ27_003192"
     CDS             7615..7842
                     /locus_tag="JNZ27_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665419.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7880..9121)
                     /gene="agp"
                     /locus_tag="JNZ27_003193"
     CDS             complement(7880..9121)
                     /gene="agp"
                     /locus_tag="JNZ27_003193"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY8665420.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGLLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9414..10670)
                     /locus_tag="JNZ27_003194"
     CDS             complement(9414..10670)
                     /locus_tag="JNZ27_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY8665421.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLNYLNRYAT"
     gene            10931..11851
                     /gene="cbpA"
                     /locus_tag="JNZ27_003195"
     CDS             10931..11851
                     /gene="cbpA"
                     /locus_tag="JNZ27_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY8665422.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11851..12156
                     /gene="cbpM"
                     /locus_tag="JNZ27_003196"
     CDS             11851..12156
                     /gene="cbpM"
                     /locus_tag="JNZ27_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY8665423.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12249..12665)
                     /gene="torD"
                     /locus_tag="JNZ27_003197"
                     /pseudo
     CDS             complement(12249..12665)
                     /gene="torD"
                     /locus_tag="JNZ27_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
     gene            12722..>12795
                     /locus_tag="JNZ27_003198"
     CDS             12722..>12795
                     /locus_tag="JNZ27_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8665424.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTA"
BASE COUNT         3064 a         3258 c         3216 g         3257 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaacagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcgacta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg gaatacacca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg cttttgatca acataataac gtgctgtttg ccgatgaccc aaaggcgcaa
     2941 acccgccacg ttctggaaac tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg
     3001 gatgtgacct tcaacagcat ctttattacc gactggaaaa attacgccgc gattaacgaa
     3061 atctacgccg agttttttcc gggtgataaa ccggcgcgat tctacattca gtgcggactg
     3121 gtaaaacctg acgcgctggt ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc
     3181 gatgaaactt tcactctcac ctccccctta tgctgatgca cccgtagtgg tgttgatttc
     3241 gggtcttggc ggtagcggca gttactggtt accgcaactg gcggtgctgg agcaggagta
     3301 tcaggtagtc tgttacgacc agcgcggcac cggcaataat cccgacacac tggcagaaga
     3361 ttacagtatc gcccagatgg cagcggaact gcatcaggcg ctggtagccg cagggattga
     3421 gcgttacgcg gtggtcggcc atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga
     3481 ttatcccgcg tcggtaacta tgctggtcag cgttaacggc tggctacgaa taaacgccca
     3541 tacgcgccgc tgttttcagg ttcgcgaacg attactgtat agcggcggcg cgcaagcatg
     3601 ggtggaagcg cagccgttgt tcctctatcc cgccgactgg atggcggtcc gcgcacctcg
     3661 cctggaggca gaagacgcgc tggcactggc gcattttcag ggcaaaaata atttactacg
     3721 tcgacttaac gccctcaaac gcgctgactt tagtcaccat gcggatcgca tccgctgccc
     3781 ggtgcaaatc atctgcgcca gtgatgatct gctggtgcca acagcatgtt ccagtgaact
     3841 tcatgccgcc ctgcccgata gccagaaaat ggtgatgccc tatggcggac acgcctgcaa
     3901 cgtgaccgat cccgaaacgt ttaatgctct gttactcaac gggcttgcca gcctgttaca
     3961 tcaccgtgaa gccgccctgt aaggaattgc tatgaacgaa gccgttagcc caggtgtgct
     4021 tagcaccctg ttcaccgatg cccgcactca caacggctgg cgggagacac ccgtcagcga
     4081 tgagacgtta caggagcttt atgccctgat gaaatggggg ccgacatcag ctaactgttc
     4141 tccggcacgg atcgtgttta tccgcactgc agaaggaaaa gaacgtctga gcccggcgct
     4201 ttccagcggc aatctgcaaa aaaccctgac cgcgcccgtc accgctatcg tcgcttggga
     4261 cagtgaattt tatgaacggt taccactact gtttccccac ggtgatgccc gcagttggtt
     4321 tacctccagc ccacaacttg ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc
     4381 ctatctgatc gtcgcttgcc gtgcgctggg actggatacc ggcccgatgt cgggttttga
     4441 ccgtcaacac gttgacgacg ccttttttgc gggcagcacg ctgaagagca atctgctgat
     4501 taatatcggc tatggcgata gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga
     4561 agaagcctgc gggctgttgt aaggagcaaa ctatgaacat agttgatcaa caaacctttc
     4621 gcgatgcgat gtcctgtatg ggcgcggcgg tcaatatcat caccacggac ggtccagccg
     4681 ggcgcgccgg gttcaccgcc agcgccgtct gcagcgtgac cgacacgccg cccacattac
     4741 tggtgtgcct gaatcgtggg gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt
     4801 gtgtaaatac actgagcgcc gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc
     4861 ccatggaaca ccgctttgcc gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac
     4921 tggaagaggc tctggtttcg tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc
     4981 acgacattct gttttgcgcc atcgaagcga ttcatcgtca caccacaccc tacgggctgg
     5041 tgtggttcga tcgcagttat cacgcgctga tgcgccctgc ttgttaacct ccttaaggag
     5101 acagctgatg gcaatgttcg gttttcctca ctggcagtta aaatcgacct ctacagagag
     5161 cggcgtggtc gcgccggatg aacgattgcc gtttgctcag acggcagtaa tgggcgttca
     5221 acacgcggtg gcgatgtttg gcgcgacggt gttaatgccg attctgatgg ggctggatcc
     5281 caatctttcc attttaatgt cggggatcgg cacgttactg tttttcttta ttaccggcgg
     5341 gcgcgtcccc agttatctcg gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac
     5401 gggctttaac ggtcagggca ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc
     5461 ctgtgggctg gtttataccg tcattggcct ggtggtgatg aaaattggta cacgctggat
     5521 tgaacgacta atgccgccgg tggtgacggg tgcggtggtg atggcgattg gcctgaactt
     5581 agcgcctatc gcagtgaaaa gtgtatcggc ttcggccttt gatagctgga tggcggtaat
     5641 gacggtgctg tgtattgggc tggtggcggt gtttacccgt ggcatgatcc agcggttatt
     5701 gattctcgta ggattaattg tcgcctgtct gctgtatggc gtgatgacca acgttttagg
     5761 gctgggaaaa gccgtcgatt ttactctcgt cagctatgct gcctggttcg gtctgccgca
     5821 tttttccact cctggtttta atggccaggc gatgatgttg attgcgcccg tggcggtgat
     5881 tctggtggcg gaaaacttag gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat
     5941 ggacccgtat atggggcgag cattcgtcgg tgatggtctg gcaacgatgc tttccggctc
     6001 tgtcggcggt agcggcgtca ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa
     6061 agtctactcg acgctggtgt ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc
     6121 gccgaaattt ggcgcgctga tccataccat tccggccgcg gtgattggtg gtgcatcaat
     6181 tgttgtgttc gggctgattg ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga
     6241 tttaagccag aacggtaatt tgattatggt cgcagtaacc ttcgttctgg gcgcgggtga
     6301 ttttgctctc acgcttggag gttttacgtt gggagggatc ggtacagcaa cctttggcgc
     6361 gattttactc aatgcgttat taagcagaag gttggttgac gttccaccgc cagaagttgt
     6421 tcatcaggag ccataaacag aaaaccgccg ggttaccggc ggtgatgact ttttgacgag
     6481 cttaagaaaa aaatgcattc gtcattgatt agcccggcta gttgtcgctt ttgccgtgac
     6541 tgctcttgcc acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc
     6601 cggaaccgcc tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc
     6661 atagaattaa cgcggcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt
     6721 gaactgacgc gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata
     6781 gatatcatct ccaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat
     6841 aaaaatgtgt cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa
     6901 gtggttgtga aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat
     6961 cgctacggaa tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta
     7021 ttattccatg tacggacata ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa
     7081 agtggatggt gcagaagttg tcgttaagcg tgtaccggaa accatgccgc cgcaattatt
     7141 tgaaaaagca ggcggtaaaa cgcaaactgc accggttgca accccgcaag aactggccga
     7201 ttacgacgcc attatttttg gtacacctac ccgctttggc aacatgtccg gtcaaatgcg
     7261 taccttcctc gaccagacgg gcggcctgtg ggcttccggc gcactatacg gaaaactggc
     7321 gagcgtcttt agttccaccg gtactggcgg cggtcaggaa caaaccatca catccacctg
     7381 gacgaccctt gcgcatcacg gcatggtgat tgtccccatt ggctacgcag cgcaggaatt
     7441 atttgacgtt tcacaggttc gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg
     7501 tgacggctca cgccagccaa gccaggaaga actgtctatt gctcgttatc aaggggaata
     7561 tgtcgcaggt ctggcagtta aacttaacgg ctaatcttca acaggaggat acgcatgcca
     7621 actcaagaag cgaaagctca tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg
     7681 gaaatagccg aggccatttt cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt
     7741 tgggaagaag gtagtgatga agtgttagtc aaagcctttg ctaagaccga taaagattcg
     7801 cttttttggg gcgaacagac catcgaacgt aaaaacgttt aaccagcgac tcccccgctt
     7861 ctcgcggggg agttttctgt tatttcaccg cctcattcaa cacgctatca aacttatcca
     7921 tcgggcagaa accgttggca tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg
     7981 caggtgcctg cagggttaac gcatcggcat tacgtaactg ttccgcactc tggtacacat
     8041 attcaatttt catcaaatcg cgattggctt tgctgtcatg ccaacgctgg aaaacgattt
     8101 tgccgccaat cggcgtgcgt tcgttctggt catgcaactg atacggtttg aaatccagcg
     8161 ccgttaacag agaggctatg ttggagtcgt gcccaactaa caccgtgatt ttcggagcac
     8221 tggcgcgatc ggtgaccagg gctttgtcga tataactgac cagcggtttc gcaacattgc
     8281 gcgccacttc cggcgaggta aacagactgt cctggtaacc gtttttcagc ttcgacaata
     8341 ccttccactg ctggtcagat ttgatttctc cccaggccac ctgatccatc ggaaaacctt
     8401 cgtagtattg caaagtaaac gcatctacca gcgagttgcc gactttcagc agcccggaaa
     8461 cacctggttc ttgttgatac ttggcgctaa aggtattttt gccatccacc agcgaacact
     8521 gttgtttctc tttacaggca ggggaatctt tatagttaac gattttttcc agtagctggt
     8581 agctgtcggt aagctggagt ttgctgagct ctttctccat tgccgccacc gcctgttcac
     8641 tgaatgcggc ggaatcatcg gtgatcatcg ggttaaaggt tgggtccata gtgcccattt
     8701 tttcctggtg atgcacagga atatcacacc cagggaatgc accagtaata aagaactggg
     8761 cggtagcgac ggtacgttgc agactattgg cataggcata aacggtgtcc ggcggcgggc
     8821 attcccccga tttcactatc ccctgctctg ccagccattc acgcatgtaa tggcccatat
     8881 acacttcgag cacgccacct ttggtggtga gttgcccacc ggggacgtcc cattcgggcc
     8941 atttattcgg cgtcgactgc tccagcacac tgccattgtt cgccagcggc gcacgtaagt
     9001 tatggcggct catcatgagc acttgctgta gctgatagcc ttccggtacg gtttgtgcct
     9061 gagcatttga agcgagtaaa actatccctg ccacagttgc ggcgattagc gttttgttca
     9121 ttcctggcac ctcttttgtt atcaatcatt aaagagtgtg acagaaatat gacttttttc
     9181 cgggaacagg ttcgcaaaat ggcttgttga acgcctgttt ttctatatac atcttcaaaa
     9241 acaggcaagg tttgacgcaa actatgcctg attagcccct gcctcgcgta cgccgctgat
     9301 aattgcacct gtggctaaac agaatatctg cggcgttacc aatacagaga caatatttac
     9361 caataaaaaa gtgtgagact aataacaatg gtttttagca tatgcctttg ccattaagtt
     9421 gcataacgat ttaaatagtt aagttgcgca ttcatttcct tattttttaa cttataagcc
     9481 tgagaaaaaa gttcaggagg gaggtaaaca tcataatgat tgccagtgtt tattaaataa
     9541 ccatactcaa gatcttcatc tgttgaaaag gattcatttc ccaaggagat tgatgccaca
     9601 ttactattaa caataaataa tgagcaaatg ctatctgaaa gaacagcaag aaaattcttc
     9661 accgctttaa tcctatccga cgtgtcggta tctaaagcaa gcaaattctt gttatcatta
     9721 aatataaact cgggtaaatg cagtatgcat tttttgtcat tcaagcaact atcaatgaat
     9781 tgttttttaa aaaacagaaa gctccatgac ataaaactac ccacaccctg aattttgttg
     9841 tcagattgca gatcaacgcc aaataatgtc tttacatctt ccctgagatt atagaatgcc
     9901 gtatcaacaa gagcctcgat cgtttgttta tcaacaattt taataaactc ttcgtatact
     9961 tgcttttgcg tcttatggct gcccagcgcc catgaatact catgttcttt taaaaccagt
    10021 atggctctta acagacaata tccatcgcca tctactggag cgtattttaa tccatatgtt
    10081 ttattaattt cactctgaat aaaagatatg ccagacaaat caataccagt atctaaatca
    10141 gatattctcg gcatatttat ctgttctggc tggatactca tattttcatc atctaaacaa
    10201 tactcagaaa aaacatcatc acacgcatta atgttttcaa tatattccat aaagccatac
    10261 caataataac ctttaaaatc tgtgatagac tctgaacctc tgcccggcaa ggacactcta
    10321 acaatatcaa catcttgaac atattgtata gtatatgtaa tacctcctga aacccctgaa
    10381 taaatttgtg tatcattagt acgcaatgtt ctcataaata atgatagatc attatcattc
    10441 tgcatttcct caaattcttt ttctagtgat tcacattcca gacatggagt atcctcttca
    10501 tttactttac gtctgagttc attgtacaac cgcataaaac acaccagtgt gttttcaaaa
    10561 agcgtgattg aatatgtttg atttttttcg tactcatttt ttatatcgtt ccaggcctgt
    10621 ttcaaataat tatttgcagt gcaactaatt ccatgtatat tactacccat atatagcgtc
    10681 tataaaattt aataaataat gacgccctag ttaaacttaa agtgcctggt tcaactatca
    10741 aaaatcgctc accctttttc acctgtttaa aatatgttca gcaacccatc ttgatggcga
    10801 cctcctctcc gcgatgattt caataacata ttctgtgttg gcatatgaaa ttttgaggat
    10861 taccctacac ttataggagt taccttacag gggttccttc aatttgtgtt gatttacgcg
    10921 agataacgct atggaattaa aggattatta cgccatcatg ggcgtgaaac cgacggacga
    10981 tctcaagaca atcaagaccg cctatcgtcg actggcccgt aaataccatc ctgatgtcag
    11041 caaagaaccg gatgccgaag cccgcttcaa agaggtcgct gaagcctggg aagtgttaag
    11101 tgatgaacaa cgtcgcgctg agtatgatca gatgtggcaa catcgcaacg atccgcaatt
    11161 taaccgtcag ttccatcatg gcgacggtca gagttttaat gccgaagatt ttgacgatat
    11221 cttctcgtca attttcggtc agcatgcccg ccagagccgt caacgccccg ccacacgcgg
    11281 ccacgatatt gaaatcgaag tggcggtatt cttcgaagaa acgcttactg agcataagcg
    11341 taccatcagc tataacctgc cggtttataa cgcctttggc atgatcgaac aggaaatccc
    11401 gaaaacgctg aatgtgaaga tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa
    11461 aggccagggg acgccgggcg aaaacggcgg tccaaatggc gatttgtggc tggtgattca
    11521 tattgcgcca catccgctgt tcgatattgt cggccaggat ctggaaattg tggtgccggt
    11581 tagcccgtgg gaagcggcgc tgggtactaa agtcaccgtt ccaacactga aagaaagcat
    11641 tttgctgact atcccgccag gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg
    11701 tctggtgagc aaaaaacaga ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc
    11761 gaaaccggat gaaaacactg ccgcactgtg gcagcaactg gcagacgccc agtcgtcttt
    11821 tgatccacgt aaagattggg ggaaagcata atggctaatg ttacggtgac ttttactatt
    11881 accgaatttt gcctgcatac cggcatctct gaagaggagt tgaatgaaat tgtcggtttg
    11941 ggggtggttg aaccgcgtga gatccaggaa acaacctggg tatttgacga ccatgccgcc
    12001 attgtggtgc aacgcgcggt acgcctgcgt catgaactgg ctctggactg gccggggatc
    12061 gcggtggcgc tgacgttaat ggatgatatt gcgcacctga agcaggaaaa ccgcctgcta
    12121 cgccagcggc tttcccggtt tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac
    12181 gcatccggca actgcgccat aatgcctgat gcgacgctaa acgcgtcttt tcaggcgcat
    12241 aaacgacgtt atctgttttg gtggtcgcac tccactaaca ccagcaataa ctggcttagt
    12301 gccgcgtaaa aaccaaagct gtcatactgt cgacaacgcg cagcaaactc tggtaaccat
    12361 tgccgtagcg ccgtcagtgt tttttgccgc aaactgtcga ttcttcgcgc aggaacggtc
    12421 ccctctccca gcgaaaaatg cagataactc aacaactcga gatagatcgc cagatgatct
    12481 gccggttcgt tgaaattgcc actggtttcc atccctgcct caactaacaa gcgtttaatc
    12541 tcttgctcgt cctgtttgta ggccgatgca tacggcagcg ccgcttgttt gtcggtcatc
    12601 agaaacaggc cgcaaaagtc agcggccagt tccagacggg catcgtcacg tactgtcagc
    12661 gtggcaggta atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtacttg
    12721 cgtggcttcc atttctatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa
    12781 cggcaaaagc actgc
//
LOCUS       DADMFL010000109        12556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.110, whole genome shotgun sequence.
ACCESSION   DADMFL010000109 DADMFL010000000
VERSION     DADMFL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.110"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..600)
                     /locus_tag="JNZ27_003199"
     CDS             complement(<1..600)
                     /locus_tag="JNZ27_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8665425.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIVLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNZ27_003200"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNZ27_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY8665426.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNZ27_003201"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNZ27_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY8665427.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNZ27_003202"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNZ27_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY8665428.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="JNZ27_003203"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="JNZ27_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="JNZ27_003204"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="JNZ27_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY8665429.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="JNZ27_003205"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="JNZ27_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY8665430.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7233
                     /gene="fliI"
                     /locus_tag="JNZ27_003206"
     CDS             5860..7233
                     /gene="fliI"
                     /locus_tag="JNZ27_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY8665431.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVITAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7252..7695
                     /gene="fliJ"
                     /locus_tag="JNZ27_003207"
     CDS             7252..7695
                     /gene="fliJ"
                     /locus_tag="JNZ27_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY8665432.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7692..8819
                     /gene="fliK"
                     /locus_tag="JNZ27_003208"
     CDS             7692..8819
                     /gene="fliK"
                     /locus_tag="JNZ27_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY8665433.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8924..9388
                     /gene="fliL"
                     /locus_tag="JNZ27_003209"
     CDS             8924..9388
                     /gene="fliL"
                     /locus_tag="JNZ27_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY8665434.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9393..10397
                     /gene="fliM"
                     /locus_tag="JNZ27_003210"
     CDS             9393..10397
                     /gene="fliM"
                     /locus_tag="JNZ27_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY8665435.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10394..10807
                     /gene="fliN"
                     /locus_tag="JNZ27_003211"
     CDS             10394..10807
                     /gene="fliN"
                     /locus_tag="JNZ27_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY8665436.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10810..11175
                     /gene="fliO"
                     /locus_tag="JNZ27_003212"
     CDS             10810..11175
                     /gene="fliO"
                     /locus_tag="JNZ27_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY8665437.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11175..11912
                     /gene="fliP"
                     /locus_tag="JNZ27_003213"
     CDS             11175..11912
                     /gene="fliP"
                     /locus_tag="JNZ27_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY8665438.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11922..12191
                     /gene="fliQ"
                     /locus_tag="JNZ27_003214"
     CDS             11922..12191
                     /gene="fliQ"
                     /locus_tag="JNZ27_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY8665439.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12200..>12556
                     /gene="fliR"
                     /locus_tag="JNZ27_003215"
     CDS             12200..>12556
                     /gene="fliR"
                     /locus_tag="JNZ27_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="FliR, with proteins FliP and FliQ, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY8665440.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFIDPGSH"
BASE COUNT         3117 a         3257 c         3317 g         2865 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agaacaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgagcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag gacgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcaaaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggcccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     6541 ggacgtgcac gctcagtggt gattaccgct ccggcggatg tttctccgct cctgcgaatg
     6601 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     6661 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     6721 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     6781 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     6841 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     6901 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     6961 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     7021 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     7081 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     7141 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     7201 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     7261 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgctcgc
     7321 ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag aacagctcaa aatgctgatc
     7381 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
     7441 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     7501 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
     7561 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     7621 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     7681 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
     7741 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
     7801 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtggca acagataagc
     7861 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
     7921 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
     7981 cgttaaccac cgctcagaca atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     8041 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
     8101 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
     8161 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
     8221 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
     8281 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
     8341 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     8401 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caagggcaac
     8461 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
     8521 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
     8581 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     8641 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     8701 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     8761 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
     8821 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     8881 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     8941 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     9001 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     9061 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
     9121 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
     9181 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     9241 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     9301 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     9361 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     9421 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     9481 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     9541 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
     9601 tcaatctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccatacc
     9661 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
     9721 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
     9781 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
     9841 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
     9901 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
     9961 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    10021 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    10081 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    10141 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    10201 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    10261 atcgcatcat cgcccatgtt gacggcgtcc ctgttctgac cagtcagtac ggcactctca
    10321 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    10381 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    10441 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    10501 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    10561 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    10621 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    10681 gctggatatt ctgaacaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    10741 taaatatggc gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    10801 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    10861 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    10921 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    10981 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    11041 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    11101 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    11161 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    11221 tgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    11281 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    11341 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    11401 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    11461 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    11521 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11581 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11641 ccggcccgtt gcaggggcct gaagctgtac cgatgcgcat tttgctcccg gcctacgtga
    11701 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11761 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11821 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11881 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11941 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    12001 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    12061 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    12121 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    12181 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    12241 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    12301 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12361 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12421 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12481 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttat
    12541 cgatcctggc agccat
//
LOCUS       DADMFL010000110        12506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.111, whole genome shotgun sequence.
ACCESSION   DADMFL010000110 DADMFL010000000
VERSION     DADMFL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.111"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(67..2016)
                     /locus_tag="JNZ27_003216"
     CDS             complement(67..2016)
                     /locus_tag="JNZ27_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8665441.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="JNZ27_003217"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="JNZ27_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="JNZ27_003218"
     CDS             complement(2437..2793)
                     /locus_tag="JNZ27_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8665442.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="JNZ27_003219"
     CDS             complement(2793..4289)
                     /locus_tag="JNZ27_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8665443.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVIPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="JNZ27_003220"
     CDS             complement(4286..4447)
                     /locus_tag="JNZ27_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY8665444.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5020)
                     /locus_tag="JNZ27_003221"
     CDS             complement(4457..5020)
                     /locus_tag="JNZ27_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665445.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(5017..5541)
                     /locus_tag="JNZ27_003222"
     CDS             complement(5017..5541)
                     /locus_tag="JNZ27_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665446.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5507..5923)
                     /locus_tag="JNZ27_003223"
     CDS             complement(5507..5923)
                     /locus_tag="JNZ27_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8665447.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5920..6243)
                     /locus_tag="JNZ27_003224"
     CDS             complement(5920..6243)
                     /locus_tag="JNZ27_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8665448.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6288..8018)
                     /locus_tag="JNZ27_003225"
     CDS             complement(6288..8018)
                     /locus_tag="JNZ27_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8665449.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARPETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7996..9237)
                     /locus_tag="JNZ27_003226"
     CDS             complement(7996..9237)
                     /locus_tag="JNZ27_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8665450.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9237..9419)
                     /locus_tag="JNZ27_003227"
     CDS             complement(9237..9419)
                     /locus_tag="JNZ27_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665451.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9431..11188)
                     /locus_tag="JNZ27_003228"
     CDS             complement(9431..11188)
                     /locus_tag="JNZ27_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8665452.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11188..11670)
                     /locus_tag="JNZ27_003229"
     CDS             complement(11188..11670)
                     /locus_tag="JNZ27_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8665453.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11818..12168)
                     /locus_tag="JNZ27_003230"
     CDS             complement(11818..12168)
                     /locus_tag="JNZ27_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8665454.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12211..>12506)
                     /locus_tag="JNZ27_003231"
     CDS             complement(12211..>12506)
                     /locus_tag="JNZ27_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8665455.1"
                     /translation="QRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQS
                     VREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT         2823 a         3716 c         2896 g         3071 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agccatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacctggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggaatgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcagggcatt cacgtcctct gcctgtcgcg tgtcttcttc cgtgatttca tacttcgccg
     4621 taaaatcaaa ctggtaatac agttcgtaac ggttcagatc cagcagggtg ccaccgacat
     4681 aaacaatctc gcccccttgc gggtccggca tccatccaag cagggctttc cagagttctc
     4741 tccggacatc atgaacggcg tcataggctg cccactgccc tttttcatca cgctcattgc
     4801 tgagcaccac aatcacggag aagccttcag tcaaatcctg ccagtaatcg gtctgtgatt
     4861 tttgttcccc cggcgcatcg tcagagggaa ccacataggc agcaggcagg cgaagttttc
     4921 cggcatcagg gatcgcctta aactgcgccg cgccaccaac acgctcctca aaacgcgggc
     4981 atcgctcacg cagcgccgca attatcgttg tcagtttcat ttatgcttcc tttttaccgg
     5041 acgtaacgaa cgctgcagct cactggacaa cagttcctgc gtccagtgac gctgacgctc
     5101 aataacgtca gccataaagt tattacgcgg ggccagccgg aaagtcgaag aatggtgctt
     5161 cttctgccgc ttatcctttt tatccattcc atacgctgaa tggcgaacgc cgtaatacag
     5221 aaacgccgga taataaggag caccttcagg aaaacggcga ttcccctgcc cgtttttctg
     5281 gtcaggggaa attttcacca tcagtccggg gcgacgcgtc gtttttttgg gaacgtaata
     5341 accaatggag cgggccagac gcccggtctg atatcccggg ttctctcccg gaccggagcg
     5401 tccgcgttta atcaccagac gtctggcatc acgcatgtaa acgcgcccga tttgcacaaa
     5461 cgcccgacgc agacgggcgc gattaaactc cagctcctct ggttgtttaa agtcgacgtg
     5521 taaaaatgct gtctgattca tggcattcac cccgtcgtcg cgctgtacgc agttcttcac
     5581 attccagtaa taaaaaacgc cgctgaccgt tcaggtcacg tattcgccgg atccggtact
     5641 cttggccgta ataaaccact tcgtgatctg ccgtgatgtc gtgccggaaa cggatcgtaa
     5701 aataatgcgt aacgatattt tctgtctgca ctgagccctg ataagcggca gcgcctggct
     5761 gagccacctt tgcccagaca tcaaacgact ccggatacgt tggctccgta ccaaaatcag
     5821 cggtgggttc atccacacga aggcggatct ttatccggcg attcagctct ccgggatccg
     5881 gtaaaaggta agtggcactg gtctgacttt gcctgatttt catagcggga caatcctgta
     5941 agggccagca agccatctga aactcattgg cgtttccagt ttctccacat cggtgatcgt
     6001 tgagcgattt tcataaaaat gactgaccag catcagcatt gccagacgaa cgtcatcagt
     6061 cagatgcatc ccgtcaggat catcttccgg aatcgtctct tccggtgcat acaacttcct
     6121 gttcaggtat gtctctgttt ttttctgtac cgcctgtgcc agcagtttca gaaaatcgcc
     6181 gtcactgtac aaaccatcat cgagccggag atgagattta atctcctctt ctttcaggag
     6241 catattttcc tcctgtgccc gcgattacgc gggcacaaaa aaccgcatta cgcagcggct
     6301 ttctggcggg ttgcagcccc aattttcatc agcttaatcg cctgagaatc caccagcata
     6361 ccgccggttc gcttggtggt ataaaaaccc acgaacggtt tgttggtgta cgggtcacgc
     6421 aggatacggg taccgatgcg atcaacgatg gtatagccgc gtttgaagtt cccaaacgca
     6481 atggcttttg catcggcggc aatatccggc atctgctcat tctcaacgat gccataccct
     6541 gccagagaag aaggctgacc cagctcaata cccggacgcc acaaataatt tccgtcgtta
     6601 tccttcagca gacgaatggc aaacaggctg ctgttgttca tcatgaactt cgcgccgctg
     6661 cggtgcgcct tacgcaaggt gtaaatcagt ttaatgatcg catcagcggt cacgccggaa
     6721 gccgcgccgg aagcaatgtg ctgaagtttg ccaaacgcac gggtcttgtc atcttcatcg
     6781 gtggactcat aagccagaaa accttttggt tttttgctac cgtcgccact ggtaaaggca
     6841 atttcttcct gttcggcaaa ttccagcgcc agctcactgt tgatccagtc ttccacatta
     6901 aagaaagcat catcgagcat tttctgtgtg gcctgcgggt tgccgtagat ttcccccata
     6961 aagggctcaa tcagtcccag ttttgaggtg gcggtttccg gacgcgcttc cgtttccccc
     7021 acccatccgg acgtcgtgcc gcccagattc accagttttt tataatccga gccacccaga
     7081 gtgattacgg tggcctcctg gcgcatcacc acttcatctt tcagcaacgt cagaatggtg
     7141 cgatccagtt cttccggaat ggcataacca ccatcttcat cattacccac ctgcagtgcc
     7201 tttcgctcaa gctcacgcag gccatcttca cgccccttgc gcataaaacc gataaacgct
     7261 tctttatgtt ccccggcaac tttattttgc gtaccacctg ccggacgctt aacttcagcc
     7321 agctcagcct caagatcgct tttgagattt tccagttcag tcagttttcc gttgagggtt
     7381 tccacttctc cgttcagtgc agagcccaca tcctcagcgt cgcgcaggga cagtgcgcca
     7441 tagcccccgg ccatgaatgc tttggcctgg gtacgggaga gtccgacatc acgcaggact
     7501 ctttcgattt ttttctgttc ggggatttcc ccgcgggcca gtgcgttctt gacgtcgctg
     7561 atccgcgcct cgtcgttaga cgggaacgtc accagactga cttcccagag gtcgatttct
     7621 ttcagcagaa aggcttcttt gctccggtcg tattcccagt ctttcaggac gtacccaata
     7681 gaaaggccgg ttaacgaacc ggccttcatg tgtgcatgtg cacgttttgc gaggggatca
     7741 tcatcaataa gcaaccgtcc cctgacgtaa agcccgacat cgtcttcctt catttcggtg
     7801 taaacaccga tgggttcatc catgcggtgc tgccagagca gcgcaggtaa cgcttttctg
     7861 tcactccacg cccgcaggga agcagcaaat gccccggaca tcaccacatc atcgtggctg
     7921 tcctttacac caaagacgga gccatgccct tcaaactcac cggagtcact gacagatttc
     7981 agactcagcg gtacatcaag acgttgtttc gtctgcattg gcgttatcct tctgcttacc
     8041 ggctttactg ccatcggagg gtttcgtggt catgttcatc ggtgtgagat agacatcccc
     8101 accgggacgc ggattcatat cttccaggtc gcggcagtca ttgggagagt aaatccccca
     8161 gttaatcccg gtggcgtagg cttcaaaacg ggatttcata tccccgcgca gtaacgcccc
     8221 ggcgttaaat ttggcgtaaa acttcccctg tttgctctcc ctgaccagcc ctgtattgat
     8281 ccgttgttca atacgggtca gatacggcac aagggaatag ttgataaatc cgagccccag
     8341 ctcttcaata ttgttgaaag tggcgcgatc ggtgttctgc accatgtgca gcggcacgcg
     8401 gaaaagacga cagatttctt ccaactgaaa cttgcgggtt tccaggaact ggctgtcctc
     8461 ggcattcagt gccaccggct tccagtccag ccccatttcc agaatcatcg gacggtgcgc
     8521 attacccagc ccaagatgac gctcctcaaa atccctcttc atgcgctcat aagcatccgg
     8581 cgtgagcttt tgttccgtgc gcaacacacc ggatgtcacc gcaccattac caaacaacct
     8641 ggcaccgtgc tcctcggttg ccgctgccag tgaaatggcc tcgcgcgcat atgcaatggg
     8701 attcagcccg accagtccgt ccagcgtcag ggtgcgcaca tgccagattt catcctgggt
     8761 caacacatcc acggaaccat ccggaaacgt cacctgataa accggctgcc actggctgtt
     8821 cagcttcggt tccacacagc ccggatctat cggaagaagc tccacaactt cccccagcgc
     8881 cttcaccttg taagcgtaaa aattcccccg cagacacagg cagacgataa ccagctccca
     8941 gaattcctgc ggcgtcatgt agccattggg ttttgctgaa atcagcttat gcagccgttc
     9001 atccaccgcc cgtgttttaa gggtgccgct gattttgtag agactgcagg gcagcatacc
     9061 aacagactca gccagcaccc tgacgcagga atagaccgcc gtcagccgca tggcccgctg
     9121 gctgctgatc cgctttccgg tataggtgtc gtatgacaac cccagctctt ccgcaagcat
     9181 cccgggcgtt gtgacggggg tgttattttt gcgttgaaaa agcccctgga aaaacattac
     9241 tcacctccgg aggcgacccg gtgaccgcga tcgagataac gcgcaaccag ccacgaccag
     9301 cacagacaca gcgccccggc aacaacaaaa cctgccgggg gataaatcag ccatgtgcca
     9361 tacgacagca aaagcgcccc cagcacgccc accagtggcg tgagaattat cagaaacata
     9421 atgacctcgg ttaaagcgag cgaataccaa cgctgaccag atgctcagac agatccggct
     9481 ccggttcacc gccattgacc agcatccggc tcattgctgt aaacatcgca acagggccgt
     9541 cgattttggc ttccggcgtg gatttgttcg ggaagatatt gtcgtttttg tccggtttca
     9601 ccgtaacgtt agacatcatc cagttcatga ccgggtgatt actgtgatgg aaacgcccgg
     9661 catagaccag tgattccgtt tccttcatgg cctctgacag attgcggacc gtctgcggaa
     9721 cttccaccag cggtatacct tcttcagcca gcgccagact gaactgcatt gcgctccacg
     9781 ggtcaaatcc cagttccctc aggttttcac cgccaatcca ttccagtaag tcacttttta
     9841 tctgagcatg atcgataaca tcaccatccg tcagaatcag cttatccatc tccgcccact
     9901 tccggtaaag ttctgcctgc tgccgcgagc atcgttccag ccgtccttcc ggaagccaga
     9961 atttaaaatc ggcatgaaca tgcccgttat ccgttcgcca gagttttgcc gccgcacaga
    10021 tatcaatctt atgagcaagg tcaacgccga cccacatggg atacgttttc agctcatgtc
    10081 gtggggcaat gtattcgcac ttctcccact taatcatgtc catccaggca gactctgctg
    10141 ttacccacac attcatgtgt ttggtaaaaa aattcacccg cgcagagacc tgttctttcg
    10201 cttttttcgc caggcgacgc agatcatccc agcgtttaca gatgcccagg ccgggattcg
    10261 ctttctgcca gaccgtttca tcaaacggat catctccctc atcgagggtg taaataatcg
    10321 caaagtagga gtcgtctttt accgcgccct ccacatcgct gttatagcca cgcaatacct
    10381 tgatggcgta atcacgctgc tcgtaacaaa tcccttcctt gttaaaccct gccgtggtga
    10441 taccaaataa aagggactgc agacgggcac cggttgccgt ttccagaacg tcccacacgt
    10501 cacgggtttt atgtgcatgc agctcatcaa taatggcgca gtggatgttc agaccatcca
    10561 ggttgtttgc atctgaagaa agcggttcaa attttgatgc gctctgctcc tgataaatcg
    10621 ccagcttgtt gaaatcaaac aaccgcccga gtgtcgaccg ggcttttctg accatatttt
    10681 tggcgtcttc aaacacgatt ctggcctggt cacgcgtggt tgcggctgaa tacacctcag
    10741 ccccgccttc accatctgcc cccgtcatat acagaccgat acctgatgac agggttgatt
    10801 ttgcgttttt acgggcgact tcgttgtacg ctgtccggaa acggcgcacc atcaccgggc
    10861 gtccgctgcc atcgctgcgc atgacaactt ccccggtctc ttcattcacc agcggaatga
    10921 caaaaccaaa aatattaatg aggataaaca catgccagtc catcaactca atgggctgac
    10981 ctgccagcgc cccttttaca tggggcacaa atttgtagaa attcaggatg tgctgcgcac
    11041 ggggttcact gaaataaatc ccccgctctt cgccgtactt cagatcatca agaaaacgct
    11101 ggcaggccag acggacaaat tcaccagcga caatttctcc tgcaacaaca cgttcggcgt
    11161 agcggatccc gtcagccact tttgccatca gtctctcgct tttaaaagtt ctgccagcgg
    11221 atcaacatca tccggtccgg cgatatttac tttcgcccgg cttgccggtg acataccaaa
    11281 ttctgcaagc atcgcccgga tccgcttcca ggcatccgct ttcatcgcag cagccgggtg
    11341 tgccttaatc agcacatcac cgttctgcgt ttccgtgcgg taggtataac cctcaacatc
    11401 gagggtttcg cagtgatgcc ggtattcggt gtaggcttcc accagcaact cgagtgcacg
    11461 cgcatcaagc tgagaaatga tcccttccgc attcagctct tccgccattc gcctgaacca
    11521 gtactttccc tgcgccccta aatgctgcgg aattttaggg agaccttttt cgtccttttt
    11581 agcggttttt ttggcgtctt taacgggacg ctttgaggga ttgcctcgta tcaaatgcag
    11641 gcgtggcggg gttttcgggg gtcctgacat aatcgatttt acctatcaat cgcttgatcg
    11701 cattcccaaa aaaaagtttt cgaacctgcg gcgatgcgag gaagggttgg cgggcggtcc
    11761 cggacagcca gggctgcagg gatttgaccc gcccctccct acaagtgaga ataattatca
    11821 cctgattcgt tcgcgcgctg ttttcgcttt atggcagggc cagcacagac tctgcaggtt
    11881 gctgtctgcg tctgttccgc catgcgcttt cgggatgatg tggtcgacgg ttttcgcctc
    11941 gctcaccaca ccgacacgca gacacaactg acacagacct ttatcgcgct tcagaatacg
    12001 ggcacgaatc accgtccatt ttgagccata gccacgctgg tggcggctca gtccgcgctg
    12061 gtgctgcacc cagccttcac cgcgatgctt atcgcagtaa cccgaactgt ctgtggttgt
    12121 acctgcacat ccacgcttac ggcaggcacg tgggattagt gatggcataa atacctcata
    12181 ccctgcgaaa tgtttaccac gataaaaagg ctacttaatg cactgagtgc ggatatactc
    12241 ctgtgcccct tccagttgca tctgcatcgt catcagccgc tctctgaggg tgaaataatc
    12301 ccgttcagcg gtgtctgcca gtcgggggct ggttgcatta tccacgccgg aggcggtggt
    12361 ggcttcacgc actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac
    12421 atcagcgcgc agagtttcat tttcagcttt cgcatcagct aactccttcg tgtatttagc
    12481 atcgagtgca ttaacatcac gctgtc
//
LOCUS       DADMFL010000111        12403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.112, whole genome shotgun sequence.
ACCESSION   DADMFL010000111 DADMFL010000000
VERSION     DADMFL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.112"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNZ27_003232"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNZ27_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY8665456.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="JNZ27_003233"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="JNZ27_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY8665457.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="JNZ27_003234"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="JNZ27_003234"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY8665458.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="JNZ27_003235"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="JNZ27_003235"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY8665459.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="JNZ27_003236"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="JNZ27_003236"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY8665460.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVLPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="JNZ27_003237"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="JNZ27_003237"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY8665461.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECY
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="JNZ27_003238"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="JNZ27_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY8665462.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="JNZ27_003239"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="JNZ27_003239"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY8665463.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="JNZ27_003240"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="JNZ27_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY8665464.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="JNZ27_003241"
     CDS             7148..7759
                     /locus_tag="JNZ27_003241"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY8665465.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNZ27_003242"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNZ27_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY8665466.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNZ27_003243"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNZ27_003243"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY8665467.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNZ27_003244"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNZ27_003244"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY8665468.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="JNZ27_003245"
     CDS             complement(11999..12358)
                     /locus_tag="JNZ27_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665469.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
BASE COUNT         3137 a         3143 c         3094 g         3029 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgct ccccccctac
     3661 ccgctctcaa cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgctactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccc
//
LOCUS       DADMFL010000112        12313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.113, whole genome shotgun sequence.
ACCESSION   DADMFL010000112 DADMFL010000000
VERSION     DADMFL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.113"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..639
                     /locus_tag="JNZ27_003246"
     CDS             <1..639
                     /locus_tag="JNZ27_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAY8665470.1"
                     /translation="ADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAK
                     LFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASND
                     ATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIA
                     VELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            717..2258
                     /gene="xylG"
                     /locus_tag="JNZ27_003247"
     CDS             717..2258
                     /gene="xylG"
                     /locus_tag="JNZ27_003247"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY8665471.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            2236..3417
                     /gene="xylH"
                     /locus_tag="JNZ27_003248"
     CDS             2236..3417
                     /gene="xylH"
                     /locus_tag="JNZ27_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY8665472.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            3495..4673
                     /locus_tag="JNZ27_003249"
                     /pseudo
     CDS             3495..4673
                     /locus_tag="JNZ27_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(4781..5605)
                     /locus_tag="JNZ27_003250"
     CDS             complement(4781..5605)
                     /locus_tag="JNZ27_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY8665473.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            5925..7955
                     /gene="malS"
                     /locus_tag="JNZ27_003251"
                     /pseudo
     CDS             5925..7955
                     /gene="malS"
                     /locus_tag="JNZ27_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            8133..9386
                     /gene="avtA"
                     /locus_tag="JNZ27_003252"
     CDS             8133..9386
                     /gene="avtA"
                     /locus_tag="JNZ27_003252"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY8665474.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(9537..10010)
                     /locus_tag="JNZ27_003253"
     CDS             complement(9537..10010)
                     /locus_tag="JNZ27_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8665475.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(10112..10960)
                     /gene="yiaJ"
                     /locus_tag="JNZ27_003254"
     CDS             complement(10112..10960)
                     /gene="yiaJ"
                     /locus_tag="JNZ27_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY8665476.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            11161..12159
                     /gene="yiaK"
                     /locus_tag="JNZ27_003255"
     CDS             11161..12159
                     /gene="yiaK"
                     /locus_tag="JNZ27_003255"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY8665477.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            12171..>12313
                     /locus_tag="JNZ27_003256"
     CDS             12171..>12313
                     /locus_tag="JNZ27_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY8665478.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         3108 a         3082 c         3099 g         3024 t
ORIGIN      
        1 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
       61 gccttggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
      121 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattct
      181 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg
      241 aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca
      301 aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa
      361 gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg
      421 caaactatga cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc
      481 gttgaattgg gcaatggtca ggagccaaaa gcagatacct cactgaataa tggcctgaaa
      541 gatgtcccct cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagatacg
      601 gtaattaaag acggattcca caaagagaga gagctgtaag cgttacgccc cggcgcggag
      661 cgggggcgtg atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc
      721 cttatctact tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata
      781 acgtcagctt gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg
      841 gtaaatcaac gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg
      901 aaattatttt tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag
      961 gtatcgccat cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata
     1021 tcttcctggg taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac
     1081 gctatcagaa gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg
     1141 atttagggct tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc
     1201 gcttgttaat tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac
     1261 tggatattat tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac
     1321 tcaacgaagt caaagcgatt tccgatacga tttgcgttat tcgcgacgga cagcacattg
     1381 gtacgcgtga tgctgccgga atgagtgaag acgatattat caccatgatg gtcgggcgag
     1441 agttaaccgc gctttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg
     1501 aacatctgac ggcatggcat ccggtcaatc gtcatattaa acgagttaat gatgtctcgt
     1561 tttccctgaa acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg
     1621 agaccattca gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg
     1681 atggcaaaca ggtagatatt cgtaactgtc agcaagccat cgcccagggg attgcgatgg
     1741 tacccgaaga cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta
     1801 ccctcgccgc actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc
     1861 aaaaatgtat tctggaatca attcagcaac tcaaagttaa aacgtcgtcc cccgaccttg
     1921 ctattggacg tttgagcggc ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac
     1981 ttaacccgcg catcctcatt cttgatgaac ctaccagggg tattgatatt ggcgcgaaat
     2041 atgagatcta caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct
     2101 cttccgaatt acctgaagtg ctcggcctta gcgatcgtgt actggtgatg catgaaggga
     2161 aactaaaagc caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat
     2221 tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca
     2281 tccggtggct tttctgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct
     2341 atcatcgcaa tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt
     2401 aacgtctcca acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg
     2461 ttcgtcataa tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt
     2521 ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg
     2581 acgttggttc tgggactgct tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa
     2641 gtcccttcat ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc
     2701 atcaccaacg gcacgactgt atcccccacc agcgccgcga tgtcacaaat tgggcaaagc
     2761 tatctccccg ccagtaccgg cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt
     2821 tggcaatggc gcggaagaat gcgccgtcag gctttgggtt tacagtctcc ggcctctacc
     2881 gcagtagtcg gtcgccaagc tttaaccgct atcatcgtat taggcgcaat ctggctgttg
     2941 aatgattacc gtggcgttcc cactcctgtt ctgctgctga cgttgctgtt actcggcgga
     3001 atgtttatgg caacgcggac ggcatttgga cgacgcattt atgccatcgg cggcaatctg
     3061 gaagcagcac gtctctccgg gattaacgtt gaacgcacca aacttgccgt gttcgcgatt
     3121 aacggattaa tggtagccat cgccggatta atccttagtt ctcgacttgg cgctggttca
     3181 ccttctgcgg gaaatatcgc cgaactggac gcaattgcag catgcgtgat tggcggcacc
     3241 agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt tatcatggct
     3301 tcactggata acggcatgag tatgatggat gtaccgacct tctggcagta tatcgttaaa
     3361 ggtgcgattc tgttgctggc agtatggatg gactccgcaa ccaaacgccg ttcttgattt
     3421 tgataaaaat tttctcaaag ccggttacgt attaccggtt ttgagttttt gcatgattca
     3481 gcaggaaaag aaccatgttt actaaacgtc accgcatcac attactgttc aatgccaata
     3541 aagcctatga ccggcaggta gtagaaggcg taggggaata tttacaggca tcacaatcag
     3601 aatgggatat tttcattgaa gaagatttcc gcgcccgcat tgataaaatc aaggactggt
     3661 taggagatgt cgtcattgcc gacttcgacg acaaacagat cgagcaagcg ctggctgatg
     3721 tcgacgtccc cattgttggg gttggcggct cgtatcacct tgcagaaagt tacccacccg
     3781 ttcattacat tgccaccgat aactatgcgc tggttgaaag cgcatttttg catttaaaag
     3841 agaaaggcgt taaccgcttt gctttttatg gtcttccgga atcaagcggc aaacgttggg
     3901 ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg
     3961 tttatcaggg gttagaaacc gcgccagaga actggcaaca cgcgcaaaat cggctggcag
     4021 actgactaca aacgctgcca ccgcaaaccg ggattattgc cgttactgac gcccgagcgc
     4081 ggcatattct gcaagtatgt gaacatctac atattcccgt accggaaaaa ttatgcgtga
     4141 ttggcatcga taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg
     4201 ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact gttgcatcga ttattagata
     4261 aagaagaaat gccgctacag cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct
     4321 caacagatta tcgctcgctg accgatcccg ccgttattca ggccatgcat tacattcgta
     4381 atcacgcctg taaagggatt aaagtggatc aggtactgga tgcggtcggg atctcgcgct
     4441 ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac catccatgca atgattcatg
     4501 ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg atcaatgaga
     4561 tatcgcaaat gtgcggttat ccatcgctgc aatatttcta ctctgttttt aaaaaagcat
     4621 atgacacaac gccaaaagag tatcgcgatg taaatagcga ggtcatgttg tagcgatgat
     4681 gagaattgtc ggcgtcacat cagggaactc agcacagcca cctgatgcga gcaaaagcct
     4741 tatccgaccg acaaatcaga taaggctcga ggaaatgaaa ttacatatgc gccgcgatta
     4801 accgttggtt atcctggtac attgcgaaca ggtagttgtt ataactcttc cctttggtcg
     4861 agtagccctt cagcttgtga atcatcgctg tggcagtcac ttcctgatcc gctttacgca
     4921 gctgtgcacg cgatttacgg aacgaagagt aagccgggtg cgtattcagg ttagtgacat
     4981 aggcgctcac cgattctttg acagaactaa actgtgagta ccctttcact ttacccggcg
     5041 cattggtaca acgtcctttc atgcatttca tgccgaacag attgttattg ttgcgcgcca
     5101 gcttcgacgt tccccaaccg ctttctgctg cagccatcgt cgccaccata ctggtaggga
     5161 taatgtctac gcgttcaagc aaggtattcc acgggatttt tcgcgtatta ccagaccact
     5221 tcaccttgta gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt gaccattggc
     5281 cctgatactg ttttgaaatc agccagttac gttctgcagt aatggctgca ttttggctgg
     5341 taatgtaagg cattacggtc cggagaaacg cctttttcct tggtgttccg gaagggtatt
     5401 ttcgcaaatc aggaagtgaa ctactctttg cactattgcg agaatactct tgtttactgc
     5461 ttacctgttt attactagct ttagttaagt gggacttttg actcgctgtt gttgtgtgcg
     5521 tcttcgctaa cacctcactc gaaaacacca gagtgagtaa cataagaatc atcgccccat
     5581 atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg gatttaatca ttccaacacc
     5641 ttatattttt cacaaatttg agagttgaat ctcaaatcat atcaaaaata gctgtcaaga
     5701 gcaccccaag gaatagtcca aatctgaaac tatgtcacga gttaacgatt cagattggcg
     5761 ctaaatcgca gaaaatgtgg gggttatcgc aaaattcagc cgttttttgc gcgcgatcgc
     5821 tcacccttgc ttctcatcct gtggacttac cgctcaggga tgagttttgt ctggcttatc
     5881 gctggcaaac tgtctgaaat cgcagcaata aggactcatc caccatgaaa ctcgccgcct
     5941 gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc
     6001 ctgcctttag cgaacaggga acgggaacat ttgtcagcca cgcgcagttg cccaaaggta
     6061 cgcgtccact cacgctaaat tttgaccaac agtgctggca gcctgcagat gcgataaaac
     6121 tcaatcagat gctttccctg caaccttgta gcaacacgcc gcctcaatgg cgattgttca
     6181 gggacggcaa atatacgctg caaatagaca cccgctccgg tacgccaaca ttgatgattt
     6241 ccatccagaa cgccgccgaa ccggtagcaa acctggtccg tgaatgcccg aaatgggatg
     6301 gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt
     6361 attacagcca acaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcta
     6421 ccagcaacgg tttactcctg ctggaacggg cagaaactga cacatccgcc cctttcgact
     6481 ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta
     6541 atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg
     6601 gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt
     6661 tatggataag cgccccattt gagcaaattc acggctgggt cggcggcggt acaaaaggcg
     6721 atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca
     6781 atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc
     6841 gtattctctt tgatgtcgtg atgaaccaca ccagctatgc cacgctggcg gatatgcagg
     6901 agtatcagtt tggcgcgtta tatctttctg gtgacgaagt gaaaaaaacg ctgggtgaac
     6961 gctggagcga ctggaaacct gccgccgggc aaacctggca tagctttaac gattacatta
     7021 atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc agaacggata
     7081 tcggcgatta cgacaatcct ggattcgacg atctcactat gtcgctggcc tttttgccgg
     7141 atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg
     7201 atactcacgc taaagtcatc gaaggcttta cacctcgcga ttacttaacc cactggttaa
     7261 gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg
     7321 agttgcccgc ctggcagcaa ctgaaaaccg aagccagctc cgcgcttcgc gaatggaaaa
     7381 aaactaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctagg
     7441 gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg
     7501 attatcagga gcaggcggcg aaagcagtcg attgtctggc gcagatggat acgacctggc
     7561 agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata
     7621 cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg
     7681 cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc
     7741 cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca
     7801 acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg
     7861 gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg
     7921 ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt
     7981 acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg cgggataaag
     8041 caccgctcac tcttcaacag accgatttgc accccagcaa atgtagcgtt attgttacct
     8101 tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat
     8161 ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcacgc
     8221 ctggcgcgat tatgctcggc ggcggtaatc cggcgcagat cccggaaatg caggactact
     8281 tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgtaact
     8341 acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga
     8401 agttgggttg ggatatcgaa ccacagaata ttgcactaac aaacggcagc cagagcgcgt
     8461 ttttctactt atttaacctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc
     8521 tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt
     8581 ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg
     8641 attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga
     8701 atccaacagg caatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc
     8761 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
     8821 tcagtgaagc gcgcccgcta tggaatccga atatcgtact gtgcatgagt ctttccaagc
     8881 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca
     8941 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
     9001 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
     9061 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaaaatcgca
     9121 gcctgattca taaaccggaa ggagccattt tcctctggct atggtttaag gatttgccca
     9181 ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
     9241 acaacttctt cccgggactg gataaaccgt ggccgcatac gcatcaatgt atgcgcatga
     9301 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
     9361 aaagagcttg ggcagaaagt cactaaaaat tgcctgatgc gctacgctta tcaggcctac
     9421 atgatttctg caatatattg aatttggaag aatttgtagg ccggataagg cgtttacgcc
     9481 gcatctggca taaacaaagc gcactattcc ggcgttgaga aacgccggaa aacgtttcaa
     9541 acaggctgct gctgtagccg atgccgctgc actttctcga catccatgca ctgcaacgcc
     9601 tttgtcgggc aggcttcaac acaggccggc cccgtctccc gatgccagca cagatcgcac
     9661 ttaatcgccc tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc
     9721 agcatacagc ttttacagcc aatgcaacgt gtttgttcaa cgaaaatatg cccatgttcg
     9781 cggcttatcg cgtcaacagg gcagacattc gcgcacggtg catcttcaca ctgatgacag
     9841 gctactggcg tggtccagct gtggtcttta atgacacgaa tacgggaaat aaactcgtct
     9901 ggtgacaacg cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag
     9961 gtacggcaac cgatacattt cgtcgcatcc gcaataataa accggttcat gatcattcct
    10021 ttgcactaaa acagcagagt gccagagagc gcgtccctac tgctttgatc caacgaagga
    10081 aatgttaaag ttttggcact tgtccaaagc gttatgtgag cgcgcccagg tcatccctga
    10141 cagtaaatcc cagttcatta gaaatagcct gcgcggtttc acgcagtggt ttcaggagat
    10201 ttttctctcc cacctgtttc agacgtgatg tcgaaagcga aatcgacacg gcgtacggca
    10261 cccgcccatg aatatcaaac accggaacag caatacagga gacgccgagt tcgttttctt
    10321 ctctgtccat cgccgctccg ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca
    10381 gctcggtaat ggtattgtgg gttaacggct ggatctcatg ctgatggttt tcccagtatg
    10441 acttcacgta gtccgggtga ccaaacgcca tgtagatctt gcccattgcg gaacagtaga
    10501 gcggcatatg ctggccaata taggcacggg ttcgcagcat cccggttgtg ggttccagct
    10561 tataaatcaa aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt tcaccagtgg
    10621 cgatgttcag tgcctcaaga tgcggagcgg cgatatgaat gatattcagc gaagacagcg
    10681 ccttctgccc gacggcaata aatttggtag tcaggcggta actccctgcg gcgggcgcgg
    10741 tggtcacata gccgcaggac tgtaatccct gcaataagcg atggacggta ctcttattta
    10801 aaccagccag ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct
    10861 caatcagcat caacccgcga aacagactct ggcttccggc tggacgctct ttttcctgcg
    10921 ccatctcgtt ctcttttttt cccatcactt ctttccccat tttgtcgcgt cctgatggta
    10981 gcgcaaagtg tgccgtagtt cacgatctcg acagataatt tataaccact tgatttttat
    11041 gtcttttgaa attcatcaat cagattgctt tgttaaaaag tgatcgatat atttgaaatc
    11101 tagtttcgca tattgaaatt ttaagccaaa aaagcgatca aaaaaacaag gaagcctagg
    11161 atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat ttcacgcggc
    11221 gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac cgaatccggc
    11281 gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga aaacggcgat
    11341 atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga acagtgggat
    11401 gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc cattgaactg
    11461 gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg gatgcgcggc
    11521 ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg gaccaactcc
    11581 atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa cccgctgatc
    11641 gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat gttctcttac
    11701 ggcatgttag aagttaaccg cctggcaggc cgtcagctcc cggtcgatgg tggctttgat
    11761 gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg cattttgccg
    11821 atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc tactctcctt
    11881 tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata cggcatttca
    11941 caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg cgatgccaag
    12001 ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa ccaggccatc
    12061 cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa cggcatcact
    12121 gttgatgaca gcgtgtgggc caaaatccag gcgttatgag gagataagtc atgatttttg
    12181 gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag gcgctcgatt
    12241 ttctgcgcgc taccaacttc aacgtcctgg agccgggcgt tgtcgaaatc gacggcaaga
    12301 atatttatgc gca
//
LOCUS       DADMFL010000113        12173 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.114, whole genome shotgun sequence.
ACCESSION   DADMFL010000113 DADMFL010000000
VERSION     DADMFL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.114"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="JNZ27_003257"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="JNZ27_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY8665479.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="JNZ27_003258"
     CDS             complement(2005..2928)
                     /locus_tag="JNZ27_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8665480.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="JNZ27_003259"
                     /pseudo
     CDS             3145..4488
                     /locus_tag="JNZ27_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4713..6368
                     /locus_tag="JNZ27_003260"
     CDS             4713..6368
                     /locus_tag="JNZ27_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY8665481.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVVTLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="JNZ27_003261"
     CDS             6508..6732
                     /locus_tag="JNZ27_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY8665482.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="JNZ27_003262"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="JNZ27_003262"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY8665483.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="JNZ27_003263"
     CDS             complement(7326..8306)
                     /locus_tag="JNZ27_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY8665484.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVVAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="JNZ27_003264"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="JNZ27_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY8665485.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="JNZ27_003265"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="JNZ27_003265"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY8665486.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10983
                     /locus_tag="JNZ27_003266"
     CDS             10315..10983
                     /locus_tag="JNZ27_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY8665487.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11001..11507)
                     /gene="tnpA"
                     /locus_tag="JNZ27_003267"
                     /pseudo
     CDS             complement(11001..11507)
                     /gene="tnpA"
                     /locus_tag="JNZ27_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11515..>12173
                     /locus_tag="JNZ27_003268"
     CDS             11515..>12173
                     /locus_tag="JNZ27_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8665488.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         3151 a         2848 c         3059 g         3115 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgtaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgtagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctattctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctag aaacgtttcg ctggcactag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaagaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagcctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtggtt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cccgtaaact aatgagtcat gaatggtgac gctgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct accacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga ggaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgag ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa
    10441 gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa
    10501 agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct
    10561 acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa
    10621 gaagcgatag cgcagcgtaa accagtggtt accaccgctg ggccgcgtag tctgattttc
    10681 cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta tgaagtggtt
    10741 cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact
    10801 cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaagtc
    10861 gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat
    10921 attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag
    10981 tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat
    11041 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    11101 atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa atagccagga
    11161 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    11221 aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag tttgggaggt
    11281 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagcttca
    11341 aaataagcac atacatttga aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa
    11401 atctggcgtc ggtatctggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    11461 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    11521 cgattacagg catttaaatt ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg
    11581 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcacgtca gaatgagaat
    11641 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    11701 aaaaatgcca ctgaaacgca atggcttaaa gattctccct cacagccatt gcaacagtca
    11761 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccgga agcgggctgc ttttccccga
    11821 ttcaaaaagc ggggacagaa tgatgcattc cgctacccgc agggcgttaa gctcgatcag
    11881 gaaaacagcc gtatttttct gccgaaactg ggctggatgc gctaccggaa tagccgtcag
    11941 gtcacgggtg ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    12001 attcagacag aaagtgaagt atccactccg gctcaccctt cagcatcaat ggtcgggctg
    12061 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    12121 agttttcaga aaaaccagaa gacgctggcg agacttcagc gacagttaag ccg
//
LOCUS       DADMFL010000114        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.115, whole genome shotgun sequence.
ACCESSION   DADMFL010000114 DADMFL010000000
VERSION     DADMFL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.115"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            56..1327
                     /locus_tag="JNZ27_003269"
     CDS             56..1327
                     /locus_tag="JNZ27_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8665489.1"
                     /translation="MDKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRTGSFDYAAQFPDSPNLQAFGVSKKEITVKELEEKWLDLKRMEISAN
                     AFNRYESVARTMVPKIGGGRLVSSVTKEELLYIRKDLLTGYQNSTKNKAAAKGRSVVT
                     VNYYMTTIAGMFQFAADHGYLEANPFQGIKPLKRARAEPDPLTRDEFIRLIDACRHQQ
                     TKNLWSLAVYTGMRHGELVSLAWEDIDMKAGTITIRRNYTKLGEFTLPKTEASTNRVV
                     HLIQPAISVLKNQAEMTRLGKQHHIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFI
                     YKVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSARANPSFIASQMGHASAQMVF
                     NVYGAWMTDSNAEQIAMLNQKLADYVPWSPK"
     gene            1340..2110
                     /locus_tag="JNZ27_003270"
     CDS             1340..2110
                     /locus_tag="JNZ27_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY8665490.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYTLPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2163..2558
                     /locus_tag="JNZ27_003271"
     CDS             2163..2558
                     /locus_tag="JNZ27_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665491.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2599..3342
                     /gene="cmoA"
                     /locus_tag="JNZ27_003272"
     CDS             2599..3342
                     /gene="cmoA"
                     /locus_tag="JNZ27_003272"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY8665492.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3339..4310
                     /gene="cmoB"
                     /locus_tag="JNZ27_003273"
     CDS             3339..4310
                     /gene="cmoB"
                     /locus_tag="JNZ27_003273"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY8665493.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4475..6904)
                     /locus_tag="JNZ27_003274"
     CDS             complement(4475..6904)
                     /locus_tag="JNZ27_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8665494.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6929..8029)
                     /locus_tag="JNZ27_003275"
     CDS             complement(6929..8029)
                     /locus_tag="JNZ27_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY8665495.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNZ27_003276"
     CDS             complement(8417..9163)
                     /gene="cutC"
                     /locus_tag="JNZ27_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY8665496.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9177..9743)
                     /locus_tag="JNZ27_003277"
     CDS             complement(9177..9743)
                     /locus_tag="JNZ27_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8665497.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9959..11692
                     /gene="argS"
                     /locus_tag="JNZ27_003278"
     CDS             9959..11692
                     /gene="argS"
                     /locus_tag="JNZ27_003278"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY8665498.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11869..>12153
                     /locus_tag="JNZ27_003279"
     CDS             11869..>12153
                     /locus_tag="JNZ27_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY8665499.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         3031 a         3068 c         2936 g         3118 t
ORIGIN      
        1 tttaatatca tgaaatatgt aagctcgtat cgctcttggg cgtctggagg tatcgatgga
       61 taaagtcaaa tatccaacag gcgtcgaaaa ccacggcggc acattacgca tctggtttaa
      121 ttttaaaggt aaacgtgtca gggaaaatct tggtgtccct gacactgcca agaacaggaa
      181 gatcgccggg gaactgcgga catcggtatg ttttgccatc cgcacaggaa gctttgatta
      241 tgctgcacag ttccctgact cccctaacct tcaggctttt ggggtaagta aaaaagaaat
      301 tacggtgaag gaacttgaag aaaagtggct ggatctgaaa cgaatggaaa tctctgcaaa
      361 tgcattcaat cgctatgaat ccgttgcaag aacgatggtt ccgaaaattg gaggcggcag
      421 actggtgtca tcggtaacca aagaggaatt gctgtatatc aggaaagatt tgctgaccgg
      481 gtatcagaat tcaacgaaaa acaaagcagc agcaaaagga cggagcgtcg ttactgtaaa
      541 ttattacatg acgacaatcg ctggaatgtt tcagtttgct gcagatcacg gttacttaga
      601 agcaaatccc ttccagggaa ttaagcctct taaaagagcc agggcagagc cagatccgtt
      661 aactcgtgac gaatttattc gcctgataga tgcttgccga catcagcaga cgaaaaacct
      721 gtggtcattg gctgtgtaca caggaatgcg tcacggtgaa ctggtctccc tggcctggga
      781 agatatcgat atgaaagcag gaactattac tatcaggcgc aattatacga aactcggtga
      841 gttcactcta cctaaaactg aagcaagtac aaacagggtt gtgcatctta tccagcccgc
      901 tatcagtgtc ctgaaaaatc aggctgaaat gacaagactg ggtaagcagc accacatcaa
      961 ggttcaacta cgtgaatatg gacgttcagt gaatcatgaa tgtactttcg tatttaaccc
     1021 ccaggtggtt agaaaaagca aacaggtagg ttttatctac aaggtagatt ccattggcga
     1081 ctcatgggaa acagccatta agcgtgcggg catcaggcac cggaaagcat accagtcacg
     1141 acacacttat gcgtgctggt cattatctgc cagagcaaac ccaagcttca ttgccagcca
     1201 gatgggccat gcaagtgccc agatggtgtt caatgtatac ggagcatgga tgactgacag
     1261 taatgcagaa cagatcgcaa tgctgaatca gaagctggca gattatgtcc catggtcacc
     1321 aaagtgacac aagagactta ttaaaatcag tgggttagcc ttcaataccc gtcatgttaa
     1381 ctgcgtggag ggcaacacca cgctttacac cctgccgaaa cccgaggttg tcctgcgctg
     1441 gcgtgaacag accacagatg acttccgctt ctgttttaag tttccggcga ccatttcgca
     1501 tcaggcagca ttacgacatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
     1561 gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg gcccacggga
     1621 gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt atggtgtgga
     1681 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
     1741 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg catcacgtcc
     1801 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
     1861 actgacggcg acaaatccgc tgatccgttt tatcggtagt gatgatatga cgcaaaaccg
     1921 ggaattattt caggtctggt tacaaaaatt agcgcagtgg catcagacca ctacgcctta
     1981 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
     2041 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
     2101 tcttttctga atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt
     2161 gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
     2221 atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac
     2281 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgc
     2341 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
     2401 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg tttcgctggc
     2461 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
     2521 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
     2581 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
     2641 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
     2701 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
     2761 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
     2821 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg
     2881 attgaacgct gccgtcgtca tattaacgcc tataaagccc ctacgccagt agacgtcatt
     2941 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
     3001 ctgcaattcc tggaaccttc cgagcgccag gcattactgg ataaaattta tcaaggactg
     3061 aacccgggca gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
     3121 gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
     3181 atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
     3241 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
     3301 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
     3361 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg
     3421 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac acggtggaat
     3481 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
     3541 aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc
     3601 catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
     3661 actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg
     3721 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
     3781 cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
     3841 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga
     3901 aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc
     3961 atctctggca gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg
     4021 ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta
     4081 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
     4141 tagatatccg cattgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat
     4201 ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
     4261 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta
     4321 ttactgccgg atgcggcgtg aacgccttat ccggcctaca aacccttgct aattcaatct
     4381 attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata
     4441 acctcagcgc ccgtttccgg gcgctattca cttcttacgc ctgaacagca ggcttatcaa
     4501 atgccgttaa cttcggtgcg ttacccgtat atttttcgat atacaccagc gcagagttac
     4561 cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc
     4621 cgtttttaca caagccgttt tccagatctg gccatgcacc ttcatgcacg cataccacgc
     4681 cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga
     4741 cacgcaccag atcgccattc gcaataccaa atcgagcagc atcttcggtg tgaatagtga
     4801 tcggttcacg atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt
     4861 gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct
     4921 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
     4981 ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg
     5041 gatcggcgcg gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
     5101 cgatcagttt attttgctgc cagaaggcat taaacattgg catagtgacg cgttgcgcac
     5161 gggccccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt
     5221 cggtatagat ctcttttccg ccaggtttga gtaattccgc aagatcggca aacacgtcaa
     5281 aatcgttacg cgcttcaaat tgcggagcaa cagcctgctt catcggcaca atatgctggt
     5341 tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata
     5401 cgatatctgc gtgtttggct gctgcggtcc agtagcattc agaaacgacg atcatctccg
     5461 gtttctgcca ggctttgatc agacgattag tatcctggtg atgggtaaag ttaccgccac
     5521 ccgcccacca gatcattttg atattcggat acgtttgttc tttaccgtta tgctgatatt
     5581 tgccgccagg attttccagc gcatcgacga tacgggcaac aggaatcgcc gtcattccgc
     5641 catcatcagc ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc
     5701 cgccaacacg catcgggttg ccaccattgg agtaatgata agagaaacca aaaccgccgc
     5761 ccggtgtacc aatctgccct aacatcgccg ccagcgtcac cagcatccag tgtttctgtt
     5821 caccatattg ctggcgctga attccccagc ctgccatcag aattgtgcga ttcgccgcca
     5881 tcagttcagc gagtttgact atctgcgctt ccggtacgcc agtaatttcc gccgcccaga
     5941 ccgcgctctt cggcgtatta tcgctcttac ctgtcagata ctcttcaaac tgcggatacc
     6001 cggtagtgta tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg
     6061 caatccctaa catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat
     6121 cgccaaagaa ttcgatggtt tcggagcgga taggatcaat ggcaatcact ggtttgccag
     6181 attttttcag ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt
     6241 ttaaggtatt cagcgggttc attccccaca gcaccacaac ctggctgttt tccagaatca
     6301 gcggccagga ggcctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca
     6361 cctgtgctgc cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca
     6421 tataacgttg cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc cagccgtaag
     6481 atccggcaaa aatggccgat ggtccgttag ctttacgaat acgctcatgt tgctcatgaa
     6541 tcagttttaa tgcctgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg
     6601 ccggttgcag tggattgtcg agatagcttt ttctcaccat cggatgctga atacgcgccg
     6661 tggtgtgtac ctgatccgcc gccgtagact gtaaggagtt cggtatggtt ttcgccagcg
     6721 cgcctgtcga agaaacaatc ttgccgtcct tcacttctac gctcatcgct ccccaacgtc
     6781 ccgcggtgag gattttaccg cccttctctt ctgcccatgc ggggagcggt gctgccgatg
     6841 tcaccaccag cgctccagcg gcaataccgc tgtgtttaat aaattcacgt cttgttaatg
     6901 ccataacttc ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt
     6961 actggaaata ccgcgttaaa atgtccagtt cgttttcgct catgctggtt cgtgccccca
     7021 ttcctttggc aatggacggc cacgcattga cggtgtaatg gtcggcggca atagaggcat
     7081 gacaaccagc gcaataggta tcgtcaagtt tttcagcgta ttgccatagc ggtttacggt
     7141 ccgctaatgc gggatcggta agcgcaccct gtaaagacgc ctgacgccat tgattgccgt
     7201 attcgtcagc ctgccattcc ccgtttaccg tgagcgcctt gataccttct tcacttaatg
     7261 tggctagcgc cagccgttga cctgccgcca ggtagagcgt gttttcactg ccctgcattt
     7321 gataaccctg caacagaacg atcggctgtt tgccactggc atcaacgacg gtgagatcgg
     7381 taccaggatt cacggtagcc agctcgccta tgcgagaagt tttgaaagga taaatatgtg
     7441 cgccattagt cactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca
     7501 tttttatttc tggcggaaaa tgggcaatgc ctttatgaca atcgatacag gtttcgccgc
     7561 ccttttgtgc tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg
     7621 catcaaaaga atggcaacta cggcacgttg cagagtcagt tgcttttaat tctttccata
     7681 ctgtttcggc catttcctgg cgatgagttt cgaacttatc gtcactgtct attttgccgc
     7741 taacaaattc atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc
     7801 ctgactttgg aatatggcaa tcggcacatt ccgcacgtat ccctttctgg ttcgaaaagt
     7861 ggacagttcc ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg
     7921 ctgtatccga cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca
     7981 caaccgctat cagcaaaaac aataacccaa tgcgtttttt ccctcgcata gattcctcta
     8041 aataaattcc agaacaccga aatcattgta taattaaata cacaacgaat taaccgcttg
     8101 cataactcgg cacaacactg cctgaaataa tcgataaaga atatgattta ttacaatgta
     8161 atcattaatt gctaaggaat aacccagttg ctatttagaa tatatattta tcagttttag
     8221 caatttaaat cccataatca atgtgaatat aaaaaatatt tttagcacaa cgctatgcgc
     8281 actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa
     8341 aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat
     8401 gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa
     8461 ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg
     8521 acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga
     8581 cttccagcac tccagcatcg aggaagtggt gcaggttttc tgcacggact cctgctccag
     8641 ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca
     8701 gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc gcaagattat
     8761 tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg
     8821 gaccggcagc agccattatt ttttccattc gtggcatatc gacattccca tcaacatcga
     8881 gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa
     8941 tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat
     9001 gcacagggat cgtcacccgc tggcgcacgg atttcagtcc ccccaacgac ggcgttaagc
     9061 ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta
     9121 gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac
     9181 gctgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt
     9241 ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa
     9301 ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca
     9361 cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg
     9421 tgtgggtaac gtttttcccc aggccacggt agctccacat tggaaaactg ccagtgcgca
     9481 acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat cattttttct
     9541 gacaaaagct cgccacactg ttcaaaaccg cgacgccagc gctcagcggt gacgttttga
     9601 tggcaacgca aagaaatgtg atcggcagtc agcggagtga tatccaaccc cagacggcgg
     9661 gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg
     9721 tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac
     9781 cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa
     9841 cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccaccc
     9901 taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt
     9961 gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc
    10021 tgcggattgc gaaccgcaga ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc
    10081 taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca
    10141 ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg
    10201 ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc
    10261 gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc
    10321 gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc
    10381 agcagtgcgt actctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga
    10441 ctggggcact cagtttggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc
    10501 cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga
    10561 cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga
    10621 cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccaaat
    10681 cacttacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta
    10741 caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag
    10801 cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg
    10861 cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc
    10921 gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca
    10981 gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc
    11041 cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa
    11101 aacccgcgcg ggtggtacag tgaaactggc agacctgctg gatgaagccc tggagcgtgc
    11161 acgtcgtctg gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc
    11221 taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga
    11281 ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca
    11341 gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattaacg aagagcaact
    11401 ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct
    11461 gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg taatgtgtgc
    11521 ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc cgatcctcag
    11581 cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac
    11641 gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt aatcgatttt
    11701 tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc catctaaatg
    11761 tattctgaaa acggacatgc cattgttttc tcactgttga gtaagaggcc agaagcgtaa
    11821 tatccaggcc caggaaaacg ataactgttc aatttaagga ataccgaagt gtttaaattt
    11881 cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta
    11941 aacgaccatg acacttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact
    12001 gacattaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga tacgcaatta
    12061 aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgcaaaa
    12121 aaacttgata ctgaacaact taacttgctt aaa
//
LOCUS       DADMFL010000115        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.116, whole genome shotgun sequence.
ACCESSION   DADMFL010000115 DADMFL010000000
VERSION     DADMFL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.116"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            128..997
                     /locus_tag="JNZ27_003280"
                     /pseudo
     CDS             128..997
                     /locus_tag="JNZ27_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1200..1553)
                     /locus_tag="JNZ27_003281"
     CDS             complement(1200..1553)
                     /locus_tag="JNZ27_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY8665500.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNZ27_003282"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNZ27_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY8665501.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="JNZ27_003283"
     CDS             complement(3381..3674)
                     /locus_tag="JNZ27_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY8665502.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="JNZ27_003284"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="JNZ27_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY8665503.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASVPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNZ27_003285"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNZ27_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY8665504.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="JNZ27_003286"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="JNZ27_003286"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY8665505.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /locus_tag="JNZ27_003287"
     CDS             7589..8890
                     /locus_tag="JNZ27_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8665506.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="JNZ27_003288"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="JNZ27_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY8665507.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /locus_tag="JNZ27_003289"
     CDS             9320..11017
                     /locus_tag="JNZ27_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY8665508.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="JNZ27_003290"
     CDS             11054..11629
                     /locus_tag="JNZ27_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8665509.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="JNZ27_003291"
     tRNA            11736..11811
                     /locus_tag="JNZ27_003291"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2925 a         3136 c         2987 g         3105 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgattagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caagacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtgtccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatccgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aac
//
LOCUS       DADMFL010000116        12042 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.117, whole genome shotgun sequence.
ACCESSION   DADMFL010000116 DADMFL010000000
VERSION     DADMFL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.117"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1697
                     /gene="bglF"
                     /locus_tag="JNZ27_003292"
     CDS             <1..1697
                     /gene="bglF"
                     /locus_tag="JNZ27_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY8665510.1"
                     /translation="VIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVYVIS
                     GIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILGYTA
                     GKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVIPII
                     FSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAAGYL
                     WLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQV
                     GAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGAT
                     IIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLHFIT
                     SKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAILP
                     SVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVGDK
                     VNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR"
     gene            1713..3110
                     /locus_tag="JNZ27_003293"
     CDS             1713..3110
                     /locus_tag="JNZ27_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY8665511.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            3196..4812
                     /locus_tag="JNZ27_003294"
     CDS             3196..4812
                     /locus_tag="JNZ27_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY8665512.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            4839..6014
                     /locus_tag="JNZ27_003295"
     CDS             4839..6014
                     /locus_tag="JNZ27_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY8665513.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            6016..6738
                     /locus_tag="JNZ27_003296"
     CDS             6016..6738
                     /locus_tag="JNZ27_003296"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8665514.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6800..7387)
                     /locus_tag="JNZ27_003297"
     CDS             complement(6800..7387)
                     /locus_tag="JNZ27_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665515.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(7436..7909)
                     /locus_tag="JNZ27_003298"
     CDS             complement(7436..7909)
                     /locus_tag="JNZ27_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY8665516.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(7976..8641)
                     /gene="yieH"
                     /locus_tag="JNZ27_003299"
     CDS             complement(7976..8641)
                     /gene="yieH"
                     /locus_tag="JNZ27_003299"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY8665517.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8806..10143
                     /locus_tag="JNZ27_003300"
     CDS             8806..10143
                     /locus_tag="JNZ27_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8665518.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILDVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(10197..10763)
                     /gene="yieF"
                     /locus_tag="JNZ27_003301"
     CDS             complement(10197..10763)
                     /gene="yieF"
                     /locus_tag="JNZ27_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY8665519.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(10785..11534)
                     /locus_tag="JNZ27_003302"
     CDS             complement(10785..11534)
                     /locus_tag="JNZ27_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665520.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            complement(11691..>12042)
                     /locus_tag="JNZ27_003303"
     CDS             complement(11691..>12042)
                     /locus_tag="JNZ27_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8665521.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
BASE COUNT         3010 a         2829 c         3045 g         3158 t
ORIGIN      
        1 tggtcatagg taaccatgtg gccgatgtct tcctggcggt taacagcgtg gcaggccttg
       61 gcgaaaaagc gcaacaggca ccggaaaatg atgagaaagg taatctgcta aaccgctttg
      121 tttatgttat ttcaggtatt tttacgcctc tgatcggtct gatggcggca accgggatcc
      181 tgaaaggtat gctggctctg gcgctcactt ttcagtggac gaccgaacaa agtggtactt
      241 atttaatttt attcagcgcc agtgatgcct tgtttgggtt cttcccgata atcctgggat
      301 acaccgcggg caaacgcttc ggcggtaatc catttactgc catggtgatt ggtggagcgt
      361 tagtgcatcc attaattctg actgccttcg agaacgggca aaaagcggat gcgccggggc
      421 tggatttcct gggtattccg gtcacattgt tgaattactc gtcatcggtt attcccatta
      481 ttttttctgc ctggttgtgc agcattctgg aacgccgact taatgcgtgg ttaccgtcgg
      541 caatcaaaaa tttcttcaca ccattgctat gtctgatggt tatcacaccc gtcacctttc
      601 tgctggtggg gccgctatca acctggataa gcgaaatgat tgccgccggt tatctctggc
      661 tttatcaggc ggttcctgca tttgcgggcg cggtaatggg cggcttctgg caaatcttcg
      721 tcatgttcgg actgcactgg ggcctggtgc cgctgtgtat caataacttc accgtgctgg
      781 gctacgacac catgatcccg ctgttaatgc ccgccattat ggcgcaggtc ggggcggcgc
      841 tcggcgtctt cctctgcgaa cgcgatgcgc agaaaaaagt ggtggcggga tcagcggcgt
      901 tgacgggtct gtttggtatc accgaaccag cggtatatgg cgtcaacctg ccgcgtaagt
      961 acccctttgt tatcgcctgt atcagtggag cattgggggc caccatcatt ggctacgcgc
     1021 aaacgaaagt ttactccttt ggtttgccaa gtattttcac cttcatgcag accatcccgt
     1081 caacggggat tgatttcacc gtctgggcca gcgttattgg cggtgtcatt gccataggtt
     1141 gcgcatttgt tggtacgttg atgcttcatt tcatcaccag taaacgtcag ccagcgcagg
     1201 gtgccccgca agagaaaaca ccagaggtta ttacaccacc tgagcagggc ggtatctgtt
     1261 caccgatgac gggagagatt gtgccgctca ttcacgtcgc tgataccacg tttgccagtg
     1321 gcctgctggg taaaggtatc gccattctgc cctcggttgg tgaagtgcgt tctccggttg
     1381 cgggtcgaat tgcttcgttg ttcgccacat tacacgccat tggcattgag tcagatgatg
     1441 gtgtggagat cctgattcat gtcggtatcg acaccgtaaa actggacggc aaattctttt
     1501 ccgctcacgt caacgtgggt gacaaggtca atacaggcga tcggctgatt tcttttgata
     1561 tccctgctat tcgcgaggcc ggatttgatc tgacgacgcc ggtattaatc agtaatagcg
     1621 atgattttac agatgtatta ccccacggca cggcgcagat aagcgcaggt gaaccgctgt
     1681 tatccatcat tcgctaacga taaaaggagt taattacgaa agcatttcca gaaacatttc
     1741 tttggggtgg cgcaacagct gccaatcagg tggaaggtgc ctggctggaa gatggcaaag
     1801 ggatcacgac ctcagattta cagcctcatg gcgtaatggg aaaaatggaa ccgcgcatcc
     1861 tggggaaaga gaatatcaaa gatgtcgcca tcgattttta tcaccgttac ccggaagata
     1921 tcgcgttatt tgccgagatg ggcttcacct gtctgcgtat ttccattgcc tgggcgcgaa
     1981 ttttccctca gggcgacgaa gccgaaccga atgaagcagg gttagcgttt tacgatcggc
     2041 tgtttgatga aatggcgcag gcggggatca agccgctggt aacgttatcc cattacgaaa
     2101 tgctatatgg gctggtgaaa aactacggcg gttgggctaa tcgggcggtc atcgatcact
     2161 tcgagcatta cgcccgcacg gtctttaccc gctaccaaca taaagtagcg ttatggctga
     2221 cgtttaatga aatcaatatg tcgttacacg cgccattcac gggcgtgggg ctggcagaag
     2281 agagtggcga ggcggaagtt tatcaggcga tccatcatca actggttgcc agtgcgcggg
     2341 cagttaaagc ctatcatagc ctgatccccg aagcgaaaat cggcaatatg cttctcggtg
     2401 ggctggttta ccccctcacc tgccagccac aggatatgtt gcaggccatg gaagagaacc
     2461 ggcgctggat gttctttggt gatgttcagg cgcgtggcca gtatcccggc tatatgcagc
     2521 gtttcttccg cgaccacaat atcaccattg agatgactga aagtgacgca gaagatttaa
     2581 aacatactgt agatttcatc tcttttagtt attacatgac tggttgtgtt tcccacgacg
     2641 aaagcattaa taaaaatgcg cagggcaaca tactgaatat gatccccaat ccgcatctga
     2701 aaagttcaga gagggggtgg caaattgatc cggttggatt acgggttctg ttaaatacgc
     2761 tttgggatcg ttatcaaaaa ccgttattta ttgtcgagaa tggattaggc gtaaaagaca
     2821 gcgttgaagc ggatggttcg atacaggacg attatcgaat tgcctattta aacgatcacc
     2881 tggtacaggt aaatgaagcg attgccgatg gtgtggatat tatggggtac accagttggg
     2941 ggccaattga tttagtcagt gcatctcatt cacaaatgtc taagcgctac ggctttattt
     3001 atgtggatcg tgatgataat ggcgaaggaa gcctcacaag aacgcgtaag aaaagcttcg
     3061 gatggtatgc agaagtgatc aaaacgcggg ggctgtcatt aaaaaaataa ccaataaagc
     3121 accttaatta tcgtcgcatt cagaacagtc tggatgcgat acgttaattc tttctttgca
     3181 ccataaaggg atattatgtt tagacgaaat cttattacct ctgccatctt attaatggca
     3241 ccgttagcct tttctgcaca atcattggct gaatcattaa cggtggaaca acgccttgag
     3301 ttattagaaa aagcgttaag agaaacgcaa agcgaactca aaaagtataa agatgaagag
     3361 aagaaaaaat atacgccagc gacggtgaat cgtagcgtaa gtacgaatga tcaagggtat
     3421 gccgccaatc cgttcccgac cagtagtgcc gcaaaacctg atgctgtact ggtcaaaaat
     3481 gaagagaaaa atgccagtga gacaggctcg atttattctt ccatgactct gaaagatttc
     3541 agtaagtttg tgaaagatga aattggcttt agttacaacg gctattaccg ttctggttgg
     3601 gggaccgcct ctcatggttc acctaaatca tgggcgattg gttctctggg ccgctttggt
     3661 aacgaatact ccggctggtt tgatttgcag ttaaaacaac gtgtctacaa cgaaaacggc
     3721 aaacgggttg atgccgttgt gatgatggat ggtaacgttg gtcagcagta ctctaccggc
     3781 tggtttggcg ataacgccgg tggcgagaac tttatgcagt tctccgatat gtacgttacc
     3841 accaaaggtt tcctgccctt tgcgccagag gctgatttct gggtgggtaa acacggtgcg
     3901 ccgaaaattg aaatccagat gctggactgg aaaacgcagc gtactgatgc cgcagcgggt
     3961 gtaggtctgg aaaactggaa agtcggtccg ggtaaaattg atatcgcgct ggttcgcgaa
     4021 gatgtcgatg attacgatcg cagcctgcaa aacaaacagc agattaatac ccatactatt
     4081 gatttacgct ataaagatat cccgttatgg gataaagcga ccttaatggt gagtggtcgt
     4141 tatgtcacgg caaacgaaag cgcatcggaa aaagataatc aggataataa cgggtattat
     4201 gactggaaag atacctggat gttcggcaca tctttaacgc agaaatttga taaaggtggc
     4261 ttcaacgaat tctccttcct ggttgcgaat aactctattg ccaggaactt tggccgttat
     4321 gctggcgcaa gtccatttac cacctttaat ggtcgttatt atggtgatca caccggcgga
     4381 acagcagttc gtctgacctc gcagggcgaa gcctatatcg gcgatcattt tattgtggct
     4441 aacgcgattg tttactcctt cggtaacgat atttatagct acgaaacagg cgcacactct
     4501 gatttcgaat ctattcgtgc ggttgttcgc ccggcctata tttgggacca atataaccag
     4561 acaggtgttg aactgggcta tttcacccag caaaacaaag atgcgaatag taataaattt
     4621 aatgagtctg gttataaaac cacgctcttc cataccttta aagtcaatac cagtatgttg
     4681 acctcgcgtc tggaaattcg tttctacgcc acgtatatca aagccctgga aaacgaactg
     4741 gatggcttca ccttcgaaga caataaagac gaccagtttg ctgtcggtgc ccaggctgaa
     4801 atctggtggt aatggaactt aatgaaagga atattacgat gaatataaaa attgctgctt
     4861 taacgctggc tattgccagc ggtatttctg ctcagtgggc catagcagcg gatatgccag
     4921 ccagcccggc acccactatc ccggtgaaac agtatgtgac tcaggtcaat gccgataaca
     4981 gcgtgacctt tcgctacttt gcccctgggg caaaaaatgt ctctgtagtg gtgggtgttc
     5041 cggttccgga caatattcac ccgatgacca aagacgaagc aggagtctgg tcgtggcgca
     5101 cacctatcct gaaaggcaac ctgtacgagt attttttcaa tgttgatggt gtacgcagca
     5161 ttgatacagg caccgcaatg actaagcctc agcgccaggt taactccagt atgattctgg
     5221 tgctaggcag ttatctggat acgcgttctg ttgcgcatgg tgatttgatc gccataactt
     5281 accactccaa cgcattgcaa tctgaacgtc agatgtatgt ctggaccccg ccaggataca
     5341 ccggcatggg cgagcctttg ccagtgctct atttctatca cggttttggt gatactggac
     5401 gttccgctat cgatcagggg cgtatcccgc aaatcatgga taacctgctg gctgaaggga
     5461 aaattaaacc gatgctggtg gtgatcccgg ataccgaaac cgatgcgaag ggcattattc
     5521 ccgaagattt cgtgcctcag gaaagacgta aagtctttta tccgctgaat gctaaagcgg
     5581 cagatcgcga actgatgaac gatattatcc cgctgattag caagcgtttt agtgtccgta
     5641 aagatgccga tggtcgcgcg ctggcagggc tttcacaagg cgggtaccag gcgctggttt
     5701 ccggaatgaa tcatctggaa agctttggct ggctggccac attcagtggt gttaccacga
     5761 caaccgtacc ggatgaaggt gtcgcggccc ggctgaacga tccggcagct attaaccagc
     5821 aactacgtaa ttttactgtg gtcgttggtg ataaagatgt cgtaaccggc aaggatatcg
     5881 ccgggctgaa aactgagctt gagcagaaaa aaattaagtt tgattaccag gaatatcccg
     5941 gcctgaacca tgaaatggat gtctggcggc ctgcctatgc agccttcgta cagaaattta
     6001 aaataaggca ttaagatgaa attaatcatt accgaagatt accaggaaat gagccgtgtc
     6061 gcggcacacc atctgcttgg ttatatgtcg aagacgcgtc gtgttaacct ggcaattacc
     6121 gccggtagca cgcccaaagg catgtatgaa tacctcacca ccctggttaa aggtaagcct
     6181 tggtacgata actgctattt ctataatttc gatgaaattc cctttcgcgg caaagaggga
     6241 gaaggcgtaa cgattaccaa tctgcgtaat ctgtttttca cccctgcggg gatcaaagaa
     6301 gagaatatcc agaagctcac tattgataac taccgcgagc atgatcagaa actggcgcgt
     6361 gaaggcggcc tggatttggt ggtgctggga ttaggtgcag atggtcattt ttgtggcaat
     6421 ttgccgaata cgacccattt ccatgagcaa accgtggagt tcccgatcca gggagagatg
     6481 gttgatattg tggcgcatgg tgagttgggc ggcgactttt cgttagtgcc tgacagctac
     6541 gtgacgatgg ggccgaaaag catcatggcg gcgaaaaact tgctgataat tgtcagtggc
     6601 gcaggtaaag cgcaggcact gaaaaatgtg ctgcaaggtc cggttaccga agatgttcct
     6661 gcttccgtgt tgcagctgca tccttcgctg atggtaatcg ctgataaagc cgctgccgca
     6721 gaattagcgt tcggttaagc tgaagaaata aggccggatg tatgaattac gctccggcct
     6781 gttctcatta tttaaataac tacgaaaaat caccccacag tctcagcttg ccgcagtgga
     6841 gacagcggaa gagataaccc tgacaatcac cacccttttg caggcattta gctagatcag
     6901 aatttctaat accgaaattc tcacaatctt cttcaaggtt ggcaaattca tcgaggcgat
     6961 cttttatatc attccagccc acatagccga taaaagcaca gaaatcgcca caatgcgcaa
     7021 gccagaattc ctgttgccat ccatgataac ctggcgtgcg ttcaaccaac tctttcagca
     7081 tttcatcagg atatgtgttc ttcataccgg caaattcgtc ctcttcatca tactcaaatt
     7141 caacaccttc tatgctggca tcatcctgaa aactacctgc aaacttttca gcagcagaac
     7201 cgtccgcaat acacaacgga cagagatgtt caacttcatc aacgcaataa aagggacccg
     7261 agtaataaac tgacgtctgt tgttcacagc aatcgcactc tacggtttta tcctgttcaa
     7321 atgcgcctgt ttccagtggc ttgggatgat atttgaattg gggtaacggc ctgatatttt
     7381 gagtcatagt aacatcctgt ctgcttttga gtagcccaaa gataaagtat cccgcttaac
     7441 ttaaagagtc tgtttcagga catgaaatat ccaatccggg cagacgggta atgattctgc
     7501 tcatcaccac acctgccagc aatgcaggaa tgatgcggac aacgagactg tcaccagaaa
     7561 gaatgttttc aaacaactcc gaggaagcca tgcccttaat atgtacaata actgcacaat
     7621 agatctcggt gataacgacg ccacctatgc caccagccag acaacgataa tttttctgtg
     7681 acccgatctt ttccggcaga caggcgacca tcacgccggt taacaatgca ggaatagctc
     7741 ccgtagtaat cgacaataag agaaacaatg gaagtacctg tattattacc aaaagaacca
     7801 gcggttcttt tgcgaagaat atgtagagga caagaaacag aacaccaatt aacggtccta
     7861 aaaccgcaaa ataaagtccg tgatgtatta cccgacgtga aacagacata tcaaactccc
     7921 tttacttacc tttgagcgcc aatttaagcg gggtaagtga agggagtgtg aagaactatg
     7981 ccgtaatatc ccaaccacgc gctttccaca gttccggtaa ctgcgaaaga tgggtaaagg
     8041 tggtgacttt cgggtgaacg atcggcttat tgtgcgggtc ggcgcagaag tagaacactt
     8101 ccatacctgc gtcgatacca gattgcgcac cggcaactga gtcatcaacc agaatgcagt
     8161 tttctacatt tacattcatc gcttttgccg catggaacat taacgccggg tctggcttcc
     8221 agcgctgaat atcgtagccg ctgaacagtt tatccgggaa gtagtgcaac atattcagct
     8281 tgcccataga atgctgcatt ttgttatttg ggccgttaga taccacacac attggcgcag
     8341 tgatcgctga caggagcgct ccagcccctt cgatggcttc cagttctgaa tcgaacagcc
     8401 gagcgacttc tgcacggtaa acgtgttcag cttctgtttt cgctaacgta acaccatgtt
     8461 caagggaaac aatatcgata atttcgtaca gttttacacc tttgaaacgt ttgaatacct
     8521 cttcaggatc gagcgtaata ccaaattcct gaaacatcgt tacatatgcg cgggagcaaa
     8581 tgacttcact gtcgaccagc gtaccgtcgc agtcgaaaaa taccgcttct atccgggaca
     8641 tgcctttcct ttttaatcag tttaacgttt tcgtagagcc gatttgcgca acgcaatcgt
     8701 tgccgtataa gtaaatccaa taaaaaagtc tctcacaaac gcgcctattg tcgcattttg
     8761 gtatacgata gcgacggatt ttccctcctt gttcggaaat agataatgag tcatcaacat
     8821 accacccaga cttctggtca gaggatgctg gaacgcgttt ttaaactgcg cgaacatggt
     8881 actacggcac gaactgaagt gattgccggg ttcaccacct tcctgacgat ggtctacatc
     8941 gtatttgtta acccacaaat tctggacgtt gctggcatgg acaccagtgc cgtcttcgta
     9001 acaacctgtc tgattgctgc tttcggtagc attatgatgg gattgtttgc aaaccttccg
     9061 gtggcgctgg ctcctgcgat ggggcttaac gcctttttcg cttttgtggt tgtccaggca
     9121 atggggctgc cgtggcagat cggtatgggc gcaatcttct ggggcgctgt cggactgctt
     9181 cttttgacca ttttccgcgt tcgttactgg atgattgcta acattccggt aagtctgcgt
     9241 gtgggtatta ccagcggtat cggcctgttt atcggcatga tggggctgaa aaacgcaggt
     9301 gtgattgtcg ctaactcgga aacgctggtg agcatcggta atctgacttc tcacagcgta
     9361 cttctgggta tcctcggctt tttcatcatt gctattctgg cctcgcgcaa cattcacgca
     9421 gcggtgctgg tttctatcgt ggtgacgacg ctgctgggct ggatgctggg tgatgtgcac
     9481 tacaatggca tcgtttctgc accgccgagc gtaatgacag ttgtgggtca tgtagattta
     9541 gccgggtcgt ttaacctcgg gctggcaggg gtgattttct ctttcatgtt ggtcaacttg
     9601 tttgactcct ccggtacgct gattggcgtg accgataaag caggtctggc ggatgagaag
     9661 gggaaattcc cgcgcatgaa gcaggcgctg tatgtcgaca gtatctcttc cgtgaccggt
     9721 tcgtttatcg gtacttcttc cgttacggct tatattgagt cctcttccgg cgtatcggtt
     9781 ggcggtcgta ccggtctgac ggcagtggtt gttggtctgc tgttcctgct ggttatcttt
     9841 ctgtcgccgc tggcgggaat ggtgccaggc tacgctgcag ctggcgcgtt gatctacgtt
     9901 ggcgtgttga tgacctcaag tcttgctcgc gtgaactggc aggatcttac tgaatctgtt
     9961 ccggcgttta ttaccgccgt gatgatgccg ttcagctttt cgattaccga aggtattgcg
    10021 ctgggcttta tctcctactg cgtgatgaag attggtaccg ggcgtctgcg tgaccttagc
    10081 ccgtgcgtaa tcatcgttgc gctgctgttt atcctgaaga ttgtatttat cgacgctcat
    10141 taaaatcgat agcgcattaa aaacccgcca tgcatgatta ctggcgggtt ttttatttag
    10201 atcttaactc gctgaataaa ctcaccaaat gcggtcaatt gcccggtcag gtggtccagc
    10261 gtaccctgat caatcacttc tccggtttgc ggatcaactt tgttctgaat cacgccgccc
    10321 ataaattccg gcttgttcat caccattgca tcgaggaaaa ccagaatctg gcgcaggtga
    10381 tactgacagc gcgcgccgcc aatcacgccc attgagctgg tctgaatcaa caccggttta
    10441 ccggccagcg gttgatccgg caggcgggaa agccagtcga tggcattttt cagcccaccc
    10501 ggtaccgagt agttatattc cggcgtgacg atcaccacac cgtcagcctg acggatctgt
    10561 tccgccagag cttcaaccgt tgctggaaaa ccttcttcct gctgtacgtc agcgtcatac
    10621 aggggaatgt cggcaatgga aggtaacgca ttgacttcca tgctcgccgg agcaattttc
    10681 ggcagggtac gtgcaaccat gccattaaat gagcctttgc gcaggctccc cagtaacgta
    10741 accacctgca atttttcaga cataatgact cctgtggttg tctatcaatt tggcgaaaaa
    10801 gacttcacgg tagagagctg ggcaaaacgc atcatccggc tggtaggatt gttggcgcgg
    10861 ctgtgaatat ccggcaggct tttccagctg tgtttgccat ctaactccca gacactgagc
    10921 ttttcaatcg cgggcgtgac cgccacggac cacaccagca cgtcctccgc gtcgcataac
    10981 gcttctacat gatttacatg agcacatttc aggcgtccgg caatgggcag cagctgcaga
    11041 tcgcgcggat cgtcattcaa aacatcaccg gttagtttta gcacgcttcg gcgcagcgta
    11101 atgagctgcg cccgcgcttc gttaggatcg ttttgtttac tgatccatag cgattcattg
    11161 ctggaaaagg tgtggttatc gggcgcgtgt gggctatgaa acccgcgcgt cgcacgctgt
    11221 agttccatat cgaggccaca ttcaccttcg gttgttaacg ccacgccaac catattcccg
    11281 gcataggaaa tagaaaacga aggcaaattt ttatcactga aaaccggttt acctttcggc
    11341 agggtgacga tttccggcaa ttcgccaatg ccatacagca tgaacatcag ttctgcgagt
    11401 aaacctctgg acgccagaaa acggctttga cgatgagggg gaatgtttcg ggcttcttga
    11461 tgacactggg aggggagacg aacagaaatc agatgtcctt ccgttaaaat ccctcgggca
    11521 aagtgagtcg ccatttttcg ctccatgctt aatggtccat tatgaataca agagtaatca
    11581 tttactggaa tgatggacag actaaagcgg ctaaaagttc gattcagaca agcttattac
    11641 aactttttta cattttattg tgaaattggg tcgcgtttca tacgattcgg ttatgccatc
    11701 gacgcgtaaa ggtttttaat ggtttcccgt aaccagacga tcttcggatt atggctgttc
    11761 cgtttatgcc acagcagggt aaaaggaact tccagctttt tttgctggct ttcgtcaaac
    11821 gggagaggaa gagcaaccaa cggcagttga tggagttgat tgtagtactg acagtagcgc
    11881 ggcgcggtcg ccagtagcag attgtcgggt tgcgctgcca taaacagtga ctgctcgaat
    11941 tccggcaggc tcatagcaat cgtgcgttcg cgccccagct cctgtaacac attgtccagc
    12001 gccctgaggt agcctgagtt taacggacac tccttcctga aa
//
LOCUS       DADMFL010000117        11928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.118, whole genome shotgun sequence.
ACCESSION   DADMFL010000117 DADMFL010000000
VERSION     DADMFL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.118"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..2129)
                     /locus_tag="JNZ27_003304"
                     /pseudo
     CDS             complement(1..2129)
                     /locus_tag="JNZ27_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2273..2896)
                     /locus_tag="JNZ27_003305"
     CDS             complement(2273..2896)
                     /locus_tag="JNZ27_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY8665522.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2893..4581)
                     /locus_tag="JNZ27_003306"
     CDS             complement(2893..4581)
                     /locus_tag="JNZ27_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY8665523.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYVGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="JNZ27_003307"
     CDS             complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="JNZ27_003307"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY8665524.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7504..8226
                     /locus_tag="JNZ27_003308"
     CDS             7504..8226
                     /locus_tag="JNZ27_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY8665525.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="JNZ27_003309"
                     /pseudo
     CDS             complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="JNZ27_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2766 a         3307 c         2942 g         2913 t
ORIGIN      
        1 tcgacattga ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca
       61 agctgaggaa cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta
      121 tacagcaccg aaaaagtgat gttaggcgca aaggaattgc tcactggaac ccgggcttca
      181 tacagggtat cgtttaaacg ttgtagcgtt agccagtttg ccggatgcga aagcagcgac
      241 tgctgttcca cgcgatcgcg ttccagcgtc aataacgctt catcaattgg ctccgggaaa
      301 gtaatcagca tcttcgcggt ttcgcctggc tggtacagcg ttttatccgc cacgatatct
      361 accgtaccag tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga
      421 attaagccgt ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg
      481 gtaaaggatt tgccgcctga cggtagctct ccgctatggc tcgtgcggtc ttcgagacgc
      541 aaccattcat acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca
      601 accgactcgc cgctattact gtattgtgcg gcggtactta aagagtaatg cgccaggccg
      661 cgttcaatga ggatctcttt ggtggtggtg acgcgatacg ccgcgccgtc actggcggag
      721 acggttaaca aatagcggct cggtttatcg gcggcgggga gatttaacgc cacatgaccg
      781 ctggcgtcgg acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc
      841 aaatcgttac cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt
      901 tttaccggct cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt
      961 ttgaactctt ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc
     1021 acgcgaaaac tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg
     1081 gcaacggcat tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg
     1141 acatcgacgg tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg
     1201 ctgacgatgg gggatgaatg caacggatcg cggaactcgc ggcccatcac tttaacatcg
     1261 acacgatcgc ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag
     1321 atttcgcttt cgtagaagaa gttctcggag acaaaaacgc cgccttcagc atccttaccc
     1381 agaatgtatg aacgttctgg cgatatatgt tgtaactgca aagtaccgct gtcatcggtc
     1441 accccgaggg tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct
     1501 tcaccctgtt ttttacccgc ggtccatacc agaagctcgt tacctgacac tttgctaagc
     1561 gccacggtat cggaaacaaa caccaccgtc gtcgcccgat acccacctac catcgcctcg
     1621 acgaggtaca gccccggctc ttgttggccg agaggaatat aaatattacc cggctgcggc
     1681 gagatgaaat tgctggatgc gccttccagt tttatccctt gctgcggctc gaccggttta
     1741 gcctgccata gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac
     1801 tggttgttct gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt
     1861 aatgcctgag tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga
     1921 gatttgccgt accagttatc ccacagctag gtcagcgtat tgttcagccc gtcgcccaga
     1981 tattgcggtt gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg
     2041 tcaggaatac gatacaggcg aacgtccacg ccgccgtact cttccatctg atagcgccga
     2101 taatcacgcc ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg
     2161 tcagcaagca aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa
     2221 tcatcagctt tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg
     2281 ccagaaaatt taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc
     2341 gggtgtcctt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
     2401 tcgtggcgct tccggtgtgg tagatgacgt aacgccccat ccagaccatt aagtgctggg
     2461 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcttggccaa
     2521 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg tttttcccgc
     2581 tcccctgatt ccagttttgc gccagttgac gctgttcagg tgttagcgtc atctctggcg
     2641 gcaaatactg gctagataaa ccgttacttt ttagccattt actgtcgtga actttcagcg
     2701 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
     2761 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt
     2821 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
     2881 gcagcccgtg tctcatagcg gctgccaggt tatgggtagc cactgccagg cgacaccggg
     2941 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
     3001 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
     3061 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
     3121 gccatcagtg ggtattacat ccaccattgc cgggcgggtt ttattcagtg tttgcagggc
     3181 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
     3241 cacacggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     3301 ctgaccgtat cgggaactca cttcgcgaca ccagatttgc gcttcgccct gctgagtctg
     3361 gcttaccggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     3421 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     3481 ggtttgcaat tttgagtctt cataccagca cagccccgcg gcaccgtcta acgccccatt
     3541 cagcttgtcg ttttcctggc tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     3601 cggcaccgcc acacagaagc tggctccggc aggcatactg ttccagaccg gcgtaaaatc
     3661 gaaactggca tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     3721 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     3781 cagcaacctg gcgctggcca cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc
     3841 ttccaggcca aagctggctt gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc
     3901 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtactgg aaaacaccag
     3961 catcttgtcc tgatatgtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     4021 aacaggaata gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct
     4081 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     4141 cagcaccatg taatgtgaaa gatggccttg tttatcgtgc catagcgccg cctgcgcggg
     4201 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     4261 gcggcgaatg ctgccttcaa tgcccagacg atcggcatga ttctgataat agaaaacgaa
     4321 atcctcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     4381 cagagagtcg ctgtctagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     4441 caccaccagc ggcgacaacg ttttttctgg cccactgcca acatattgca acgcccagac
     4501 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     4561 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     4621 cacagccaaa ctttaccgcg ccctaatacg acaaaagccc agactttgca gcctggactt
     4681 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     4741 ctggctcgtc gtcaacgtcc acttccggag caatttcatc atccccttcc gcggcactgc
     4801 cgtcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgctgc agacccacta
     4861 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cccacgatgc
     4921 tgatttctga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     4981 cgcagtcatc tacctgcacc gcgccaacca ctaaaccgtt acgttcggta accttgatgg
     5041 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgca gtacgtttac
     5101 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     5161 cgactttatc gccttcacct aagcgaatac cgcgcacacc agtggtattg cagcccatcg
     5221 cacggacaga agactcttta aagcgcacca ctttaccttc ggcggagaac agcattactt
     5281 catcttcgcc actggtcaga tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     5341 ccactttacc ggcggtacgc agacggttga attcggtgag gacggttttc ttcacagtac
     5401 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg
     5461 cggtgatacg ttcgtcctgt tccagcggta gcaggttgac gatcggacga ccgcgcgcgc
     5521 cacgagtggc ttccggcaac tggtaaactt tcatcgaata gacgcgacca cggctggaga
     5581 agcacagaat atggtcgtgc gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     5641 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     5701 gctgatattt aacgtagccc tggtgagaga gcgtcacgac cacatcttcc tgggtgatca
     5761 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     5821 actgttcacg aaccagctcc agctcttcgc ggatcacttc catcagacga tcggcgctac
     5881 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     5941 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     6001 gttcggtcag gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac
     6061 gcgcagcatc gtcgccagca cgttccaaca tggcggcaac gttgcccagc tgccacggat
     6121 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     6181 tcgggtcaat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     6241 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     6301 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     6361 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     6421 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     6481 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     6541 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgtccggttt
     6601 tggcgtcaac ttccacttca gcacgggcgc ggatatatac cttgccgcga ccggtacggt
     6661 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     6721 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     6781 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     6841 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     6901 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     6961 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     7021 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     7081 ccatacggac gatcgtgtta taaaccgccg agtcaccatg gggatggtat ttaccgatta
     7141 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     7201 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     7261 cacggccaac aatgaccgac atcgcataat ccagatagga gctctttagc tcttcctcaa
     7321 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     7381 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     7441 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     7501 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accacgaaga gatcgctaaa
     7561 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     7621 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     7681 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     7741 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaagct gcacgcgctg
     7801 gaaagcggca ttcaggtgga ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     7861 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtacc cgatccgcag
     7921 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     7981 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     8041 atggtgccca aaggtacgca tgatgtgaag aagtttatta aaccggcaga gctgcttggc
     8101 tgggtggatc agaccagttt aaaagagcga catataaccg ggctacatta caacccgatc
     8161 actaattcct ttaagctcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     8221 aagtgaggtt gatgtttggc cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag
     8281 gcctacaaat gctctccgta ggccagataa ggcgtttacg ccgcatccgg caattgtgat
     8341 ggtcagacag cgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     8401 catcactccc tttctgccac gccacgctgc cgcgcagaga cactcgctga ctgatattgc
     8461 ccgtgactcc cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc
     8521 catcttcttc aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg
     8581 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     8641 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     8701 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     8761 cggcttccag cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt
     8821 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     8881 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     8941 tatcggcgcg agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac
     9001 caaccgcccc aagcatccac tcgccatccg tgccccagca cccgctaaac aagccgccgc
     9061 taagcagcac cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat
     9121 aacgtccgcc gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc
     9181 gctccatcat aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg
     9241 cattcagcac cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg
     9301 gcgtgggatc aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt
     9361 gcgatcgcag ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt
     9421 tgacgtagcc actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag
     9481 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
     9541 taaccaccac agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg
     9601 agtcatcgcc gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt
     9661 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
     9721 tcagatcacc attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg
     9781 tattaagctc gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt
     9841 taaatagtga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
     9901 catcgctgat gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg
     9961 cgccagtaac gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat
    10021 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc
    10081 tggcggtcag ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat
    10141 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    10201 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    10261 tatcgacatt ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct
    10321 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    10381 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    10441 catcgaccac cacgtagacg ccatctttcc cgtcaccgcc gatatgcaaa ttgtcgccac
    10501 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    10561 ggccatcatt gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac
    10621 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc
    10681 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    10741 gcgcccctgc cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    10801 tgatgccagc aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag
    10861 tgccattctg aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta
    10921 gctcagcctg attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg
    10981 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa
    11041 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    11101 gatcgccgga acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt
    11161 cattctctgt attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    11221 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
    11281 cgctattgtt ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    11341 tatcaataag atagggagcc ctcacgctat tggtatatat cgccccgcca taaccttcgg
    11401 ctgtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt
    11461 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    11521 tatatgcctg gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat
    11581 aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac
    11641 gtaaatcgac ggcaccagta tcattagtac cagaagaata gattgcgcca ctatagccgc
    11701 ctgcgacgtt accggaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
    11761 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
    11821 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggccccac
    11881 cgctggcatt attggtcata tccgaaaaaa ccagccattg cccatcgg
//
LOCUS       DADMFL010000118        11808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.119, whole genome shotgun sequence.
ACCESSION   DADMFL010000118 DADMFL010000000
VERSION     DADMFL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.119"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..845
                     /locus_tag="JNZ27_003310"
     CDS             <1..845
                     /locus_tag="JNZ27_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8665526.1"
                     /translation="GQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQDFAALFGA
                     AEAWLKSQGASKICGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHK
                     GIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGWQH
                     NWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSL
                     FPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRKID
                     ALEM"
     gene            complement(1065..1835)
                     /gene="glcC"
                     /locus_tag="JNZ27_003311"
     CDS             complement(1065..1835)
                     /gene="glcC"
                     /locus_tag="JNZ27_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY8665527.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2081..3580
                     /gene="glcD"
                     /locus_tag="JNZ27_003312"
     CDS             2081..3580
                     /gene="glcD"
                     /locus_tag="JNZ27_003312"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY8665528.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3580..4632
                     /gene="glcE"
                     /locus_tag="JNZ27_003313"
     CDS             3580..4632
                     /gene="glcE"
                     /locus_tag="JNZ27_003313"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY8665529.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4643..5845
                     /gene="glcF"
                     /locus_tag="JNZ27_003314"
     CDS             4643..5845
                     /gene="glcF"
                     /locus_tag="JNZ27_003314"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY8665530.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            5871..6275
                     /locus_tag="JNZ27_003315"
     CDS             5871..6275
                     /locus_tag="JNZ27_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY8665531.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6297..8468
                     /gene="glcB"
                     /locus_tag="JNZ27_003316"
     CDS             6297..8468
                     /gene="glcB"
                     /locus_tag="JNZ27_003316"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY8665532.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8822..10504
                     /gene="glcA"
                     /locus_tag="JNZ27_003317"
     CDS             8822..10504
                     /gene="glcA"
                     /locus_tag="JNZ27_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAY8665533.1"
                     /translation="MVTWTQMYMPMGGLGLSVLVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVLFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2830 a         2851 c         3241 g         2886 t
ORIGIN      
        1 cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgt gagcgttacg
       61 gtgaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag gattttgccg
      121 cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atctgcgggc
      181 ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt gacacaccac
      241 cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa caactgggtt
      301 atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc
      361 cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc tgcatcaatc
      421 gtcagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaactct ggctggcagc
      481 acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc gaccaactta
      541 aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca ccctgcgcgt
      601 ttattgtcgg cttgcctaac atcaacgagg cgattgccga tctgaacgga tcgctctttc
      661 ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga actgcgcgag
      721 taccgctgat gggcgtgcgc gacgagtatc agttcagccg catcggcccg gtgattgcgc
      781 tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat gcgctggaga
      841 tgtaatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc attggggcgg
      901 agccgtacaa gcgttaccga ttgtatgaaa agcaaattta ataccagatc ctgtttagcg
      961 caggggcggt cattcgggag agggtataga tgaacagatt taacaccaga tcctgttaag
     1021 tgcaggggcg gtcccctcgc cttggggata gggtaaaact ctgctcacat tcccaccttg
     1081 ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc cttccagctc gatatcgtgg
     1141 agattctttt tcacggtccg tacatgatcg cgcgctgcgc gctgcgcgac gtgcggcagc
     1201 cgttgcaaca ccgcgttgta gatccgcgca tgctggcgat cgatctgctt tttttgctgt
     1261 ggtcgatggt agagattatt tacgctggca aacactgaat taaacatcag atcggtcaat
     1321 gattgcagcg taaacaccag cacctgattg tgagaagcct gacaaatggc gagatggaaa
     1381 gcgtgatcca actgcgcatg ttcgatcagc gaaatctctt tgtggttctc actggcggcg
     1441 agcatttttt cataacagcg ggttatcaca acaaaatcag cctgcgttcc cagcgttgcc
     1501 gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt cgagcagatc gtacagcgtt
     1561 cgcggctgcg tactgaacag atggatcagc gggctggtgt cctgcacccg attaagccgt
     1621 gcgacatgag aatcgcgacc ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc
     1681 agcccttcac gcagtgcgga gcgtgagaag ccgagctttt cacacagtcg acgttccgag
     1741 ggaagcggct gaccgacctt cagtacgccg tcgataatta accgttcgat actctctgca
     1801 accacttcgc aaatagggcg acgttcatct ttcattcccg gacctcgtgc acaggtagga
     1861 ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac gcaagtgatt atgttacatc
     1921 aatttaacat taagttaacc aagacaaggt cacagagctg gaaaaaaaat ggtctgaccg
     1981 gtagctaaag agatagacga aaacgaaaag cccgcttaat aactgttcac agaagcagcg
     2041 cgcaaaaatc agctgccaca caacaaagcg aagcctactc atgagcatct tgtacgaaga
     2101 gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga
     2161 gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga
     2221 cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca
     2281 ggtgacagcg attctggctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc
     2341 aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc
     2401 gcgctttaaa gagagcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccggg
     2461 cgtatgtaac ctggcgatct cccaggccgt tgcgccgcat aatctctatt acgcgccgga
     2521 tccttcctca caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg ccggtggcgt
     2581 ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct
     2641 ggacggcgag gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct
     2701 ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact
     2761 gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc
     2821 cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat
     2881 ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc
     2941 cgaagcgatt ttgttatgcg aactggacgg cgtggagtct gacgtacagg aagactgcga
     3001 gcgggttaac tacatcttgt tgaaagcggg cgcgactgac gtccgtttgg cacaggacga
     3061 agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat
     3121 ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact
     3181 ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc
     3241 cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacctg gtgaatttgc
     3301 ccgcgcggaa gagctgggcg ggaagatcct cgaactctgc gttgaagttg gcggcagcat
     3361 cagtggggaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag
     3421 cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa
     3481 ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca
     3541 tcacggtcat ttaccttttc ctgaactgga gcgcttttga tgctacgcga gtgtgattac
     3601 agtcaggcgc tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt
     3661 cagggcagca atagcaaagc ctttttaggt cgcccagtca ccgggcaaac gctggatgtg
     3721 cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac cgctcgtgcc
     3781 ggaacgccgc tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gcttccctgt
     3841 gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg
     3901 gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt
     3961 accggcactg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac
     4021 gatctctcac ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctca
     4081 atgaaagtgt taccgcgacc gcgtgcctct ctgagcctgc gtcgggaaat cagcctgcaa
     4141 gaagccatga gtgaaatcgc cgagtggcaa ctccagccat tacccattag tggcttatgt
     4201 tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcagcg
     4261 cgtgaactgc tgggtggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa
     4321 caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg
     4381 ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggggcgtt acgctggctg
     4441 aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg
     4501 acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat
     4561 caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac
     4621 gcggaacttt gaggagcagg ctatgcaaac ccgattaacc gaagagatgc ggcagaacgc
     4681 gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc
     4741 aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta
     4801 tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga
     4861 tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa
     4921 tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg
     4981 aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac
     5041 gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc
     5101 tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct
     5161 ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct
     5221 ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga
     5281 ctatcatctt aatgcgcagg agaaagggtt ggcacgggcg cgcaataata ttgatgcctg
     5341 gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc
     5401 gttcgtcaaa gagtatgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg
     5461 tcaggtcagt gaactggcgg tcgatttagt cgaacttctg cgcgaggaac cgctggaaaa
     5521 actggcaatt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc
     5581 gcaaaagctg aacggcgaag tggaaaacgt gttacttcgt cttggattta ccttaacgga
     5641 cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga
     5701 tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat
     5761 gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctctgt
     5821 gcgtcactgg attgaaattg tataacaagc ccttgaaaag gaataaaaaa atgaaaacta
     5881 aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc
     5941 agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt
     6001 taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta
     6061 ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta
     6121 ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg gttgttgttg
     6181 atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg
     6241 tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga gaaacaatga
     6301 gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg
     6361 gagaagtttt gccgggcgtg gaactggacg ctgctgcatt ctggcacaat gttgatgaga
     6421 tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg
     6481 cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata
     6541 aatctttcct ttgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg acggtggaaa
     6601 ccacgggcat tgacagcgaa atcaccagtc aggcggggcc acagctggtg gttccggcaa
     6661 tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt
     6721 tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagtggctac gatccgcagc
     6781 gcggtgagca ggttatcgcc tgggttcgac gtttcctcga tgaatcacta ccgctggaaa
     6841 acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta cgcatccagt
     6901 tgaaaaatgg taaagaaacc acgttacgta ctccagcaca gtttgtcggt taccgtggcg
     6961 atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa
     7021 ttgatgccaa cgggcggatt ggcaaagacg atccggcgca catcaacgat gttatcgtcg
     7081 aagctgctat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag
     7141 ataaaatcct gctgtaccgc aatctgctgg gcctgatgca ggggactctg caagagaaaa
     7201 tggagaaaaa cggtcggcaa atcgtacgta aactgaatga cgatcgtcag tacaccgccg
     7261 ccgatggctc tgaaatttct ctgcacggac gctcgttgct gtttatccgc aacgtgggtc
     7321 atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggtattc
     7381 ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag aaaaactcgc
     7441 gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg
     7501 ccaacaaact gtttacccgc attgagacaa tgctcggtat ggtaccgaat accctgaaaa
     7561 tgggcattat ggatgaagaa cgccggacct cgctgaactt gcggagctgt atcgctcagg
     7621 cgcgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc
     7681 attcggtgat ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg acgccgtgga
     7741 tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggctg cgcggtaaag
     7801 cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga
     7861 agggcgacca actgcgtgcc ggggcaaaca cagcttgggt tccgtcacca accgctgcta
     7921 cgctccatgc gctgcactac caccaaacca acgtacagag cgtgcaagcc aacattgccc
     7981 agaccgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact attccggtgg
     8041 ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac aacgtgcagg
     8101 ggattctggg gtacgtggtg cgttgggtgg agcaggggat tggttgttca aaagtgccgg
     8161 atattcacaa cgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata
     8221 tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga
     8281 atatggcgaa agtggttgat cagcaaaatg ctggcgatcc ggcttatcgt ccgatggcgg
     8341 ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga
     8401 aacagccaaa cggttatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa
     8461 gtcattaata gcgtcaaaaa agccccggca agttttattg tcggggctat gatagtaact
     8521 gcggtagtac atttatcccg gaaataaccg gagcgattat cagggtttaa caatattaaa
     8581 tacccgtagc gcattactag cggcctaaaa caacaatatg gacatcttaa cgatactctg
     8641 cgcctgcgtg tgcggaacag aaacccttac ccgatatact tgttatgcca gaggtagcaa
     8701 ttgcgttagc ggcgtactgt tgctgtaaag tataacgcct atattttaca gttcagtcct
     8761 cttttgctta ttacaacctt aagtatatca agcatataaa gataataaga gactgaacaa
     8821 tatggttacc tggacccaaa tgtatatgcc gatgggagga ctggggctat ccgttctggt
     8881 cgccctgatc ccgataatat tcttcttcgt tgcactcgcg gtattacgtc tgaaaggaca
     8941 tgtcgctgga gcaataaccc ttatattatc tatcctgatt gcgatattcg cctttaaaat
     9001 gccgattgat atggcatttg ctgctgcggg ctatggcttt atttatggat tatggccaat
     9061 tgcgtggatt attgtcgcgg cggtgttcct gtataaatta accgttgcca gcgggcagtt
     9121 cgatattatc cgcagctcgg ttatctccat caccgacgat cagcgtttgc aggtgttact
     9181 gattggtttc tcctttggtg cgttgctgga aggagcggct ggctttggtg cgccggtggc
     9241 gattaccggt gcgctgctgg tgggcctggg cttcaaaccg ttatacgcgg cggggctgtg
     9301 tctgattgcc aatactgcgc cggtggcgtt tggtgcgttg ggcgtgccga ttctggtcgc
     9361 cggtcaggta acgggaatcg atccgttcca cattggcgca atggcgggac gtcagttacc
     9421 gttcctgtcg gttcttgtgc tgttctggct ggtagcaatg atggacggct ggaaaggggt
     9481 gaaagagacg tggccagcgg cgctggttgc tgggggaagc ttcgctgtca ctcagttctt
     9541 tacctctaac tatattggtc cggaactgcc ggatattact tcggcgctgg tgagtatcgt
     9601 ctcactcgct ttattcctta aagtctggcg gccgaaaaat accgaaacgg caatcagcat
     9661 ggggcaatcc gcaggtgcga tggtggtaaa taagccatct tctggcggtc ccgtgccttc
     9721 agaatatagt ctggggcaaa tcattcgtgc gtggtcaccg tttttaatct taacggtgct
     9781 ggtcaccatc tggaccatga agccgtttaa agcgttattt gctccgggcg gcgcgtttta
     9841 ttcactggtg attaatttcc agatccctca tttgcatcaa caagtgttga aagcggcacc
     9901 cattgtcgcc caaccaacgc caatggatgc ggtgtttaaa ttcgaacctc tctcggctgg
     9961 cggcaccgct atttttattg cggcgattat ctctatcttc atcctcggcg tgggcatcaa
    10021 gaaagggatt ggcgtctttg ccgaaacgct aattagtctg aagtggccga tattgtcgat
    10081 tggcatggtg ctggcattcg ccttcgtcac caactattcc ggcatgtcca ccacgctggc
    10141 gctggtactg gcaggtacag gcgtgatgtt cccgtttttc tcaccgtttc tcggctggct
    10201 gggcgtattc cttaccgggt cggacacttc ctcaaacgcc ctgtttggtt cactgcaatc
    10261 gaccacggcg cagcaaatca acgtctctga cactctgctg gtggcagcaa ataccagcgg
    10321 cggcgtaact ggcaagatga tctccccgca atctatcgcc gtggcctgcg ccgcgacggg
    10381 catggtgggc cgagaatctg aactgttccg ctacaccgtg aagcacagtc tgatttttgc
    10441 cagcgttatc ggcgttatca ccctgctgca ggcgtatgtg tttaccggga tgttagtctc
    10501 gtaaatcaat aaagccggat gaaggtaaat atcacccggc tttccatttg tctgtcgttt
    10561 atctcgccag ggaaccgttt ttacgattgg cttctttata cgtagtctcc gtttcacgat
    10621 gcgaattgtt taatatttat acaacttggc ttctgtgctg gttataaagt acccactttc
    10681 atgagatcaa ttttagtatg ttattacatt gcttcatctg ttgctttcat gacgaatggc
    10741 gttttttctc ttatttatat ttttcactag aacaatgagt agatcaaaat tcccaaaact
    10801 cccaaaaaca taagattatt cgcaaagctg attttgcaga ataatgagaa cttttaagat
    10861 ttgtcgagga tcaattttaa gcaatttatc tccacttgtt acataaaaga tgccgttagg
    10921 ttaataagtt aatcttgcta taacagtgtt cgttactgcc tattaataaa gatatttggg
    10981 atagggtaac gtggatgagg attcgaaatc atccgtcttg cggaaagtga aatgttggca
    11041 gatgttaata ttatgtatgt attttaactc caacaacaca ccaatcattt tgttagaaat
    11101 aagaaaattt atggttaaat aatttggtga ttatgaagca ttaaatcgta atgccaaata
    11161 ttgttcgctt tggagagtat tattgcctgt ttgtgtctgt tgagcagaaa aatcatctga
    11221 atttgaatac ttttattagg cgcgaagaaa tgaaaaaatc attaaaatca ttaatttata
    11281 tgaatatttc agagttggag ttcgaatgtt gacacctgag tatttcctgc taaaaatagc
    11341 cctgctttac ggatggttgt attgcaataa cacgaaagta gtaagagatt aaaggtatct
    11401 gtcagcgata actctgcaag tgatgacatt ttgtaaacca aataatgtaa atcagcaatg
    11461 tatcttttca cttaattata acaaatgtgg tattcactaa gaatctgata caagcttaca
    11521 tcacgactat tttagtctca tcgcgggata cagggatgca ccgatggatc tcagcacacc
    11581 gtaatgcaat acctgaacaa atagaacagt aaagtggttc agtattaatg aaagctctta
    11641 ttttccgttg ggaaaactta aaatagatga gaaggctttt tgatttctta tgtgatctca
    11701 tttcgagtat tgagaaattt gtgattttta aatttttcaa taagccctgt aagggattgg
    11761 ggttttatga gaaataaaga gacgtattgt ctttgtttaa acggtacg
//
LOCUS       DADMFL010000119        11785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.120, whole genome shotgun sequence.
ACCESSION   DADMFL010000119 DADMFL010000000
VERSION     DADMFL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.120"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            202..537
                     /locus_tag="JNZ27_003318"
     CDS             202..537
                     /locus_tag="JNZ27_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY8665534.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JNZ27_003319"
     CDS             630..713
                     /locus_tag="JNZ27_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665535.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JNZ27_003320"
     CDS             813..1634
                     /locus_tag="JNZ27_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY8665536.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNZ27_003321"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNZ27_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY8665537.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNZ27_003322"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNZ27_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY8665538.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JNZ27_003323"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JNZ27_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY8665539.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNZ27_003324"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNZ27_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY8665540.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNZ27_003325"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNZ27_003325"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY8665541.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JNZ27_003326"
     CDS             7279..8223
                     /locus_tag="JNZ27_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8665542.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JNZ27_003327"
     CDS             8409..9791
                     /locus_tag="JNZ27_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8665543.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JNZ27_003328"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JNZ27_003328"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY8665544.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JNZ27_003329"
     CDS             complement(10547..10882)
                     /locus_tag="JNZ27_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY8665545.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JNZ27_003330"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JNZ27_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY8665546.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2996 a         3012 c         2833 g         2944 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggcga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact attaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcaccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADMFL010000120        11595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.121, whole genome shotgun sequence.
ACCESSION   DADMFL010000120 DADMFL010000000
VERSION     DADMFL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.121"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="JNZ27_003331"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="JNZ27_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY8665547.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="yehT"
                     /locus_tag="JNZ27_003332"
     CDS             1821..2540
                     /gene="yehT"
                     /locus_tag="JNZ27_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY8665548.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="JNZ27_003333"
     CDS             2587..3057
                     /locus_tag="JNZ27_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY8665549.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="JNZ27_003334"
     CDS             complement(3098..3559)
                     /locus_tag="JNZ27_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY8665550.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="JNZ27_003335"
     CDS             complement(3684..5687)
                     /locus_tag="JNZ27_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY8665551.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="JNZ27_003336"
     CDS             complement(5684..6820)
                     /locus_tag="JNZ27_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8665552.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..9093)
                     /locus_tag="JNZ27_003337"
                     /pseudo
     CDS             complement(6813..9093)
                     /locus_tag="JNZ27_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9104..10192)
                     /locus_tag="JNZ27_003338"
                     /pseudo
     CDS             complement(9104..10192)
                     /locus_tag="JNZ27_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(10734..11141)
                     /locus_tag="JNZ27_003339"
     CDS             complement(10734..11141)
                     /locus_tag="JNZ27_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665553.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11202..>11595)
                     /locus_tag="JNZ27_003340"
     CDS             complement(11202..>11595)
                     /locus_tag="JNZ27_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY8665554.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2799 a         2944 c         3018 g         2834 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aggtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tggagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcgacggcaa ttctggcgtg ttaattacct gcggacattc
     8461 gcgccacatt tttgccagtg ccggagcgtg ccagccaccg cagacaacta acacgtcgcc
     8521 attattttgc tgcatcgccc atccaatcca gcgggccata aaggcttcgc gctgacgatt
     8581 gagagcatcg ccgggggagt cgccccgcag ttgggcaaaa tagtgcgcca gtgcagaggg
     8641 taatgcagtt tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc
     8701 catgcgggtg gcacgcagca gcaaggtctg gctttcctct tgcgtatcag gcgaatcgtc
     8761 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg
     8821 acgatcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     8881 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     8941 gtgggctaaa aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaacac
     9001 gtatcgtggc cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg
     9061 atgccggatg ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc
     9121 gggcttgata ataagcctgc cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt
     9181 aacggcgcag acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt
     9241 ccaccagttc ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg
     9301 cgtgggccac gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg
     9361 ttttcttttc gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg
     9421 cgtcaggcac tttatgcgga ataccgctat gggccagcaa acgcgccgaa gcactggcga
     9481 caagctccag ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac
     9541 gctttagcgc agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
     9601 tattaaagcc ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca
     9661 tcaccctgtc agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta
     9721 tctcttcaaa acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca
     9781 gcgcttccgt tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt
     9841 gatcttcggt agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagagg
     9901 ttgccagtaa ttcagacagg agagattttg cggtgccagg ttcccccacc aacatcaaac
     9961 cgcgtccggt ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt
    10021 tacggctaat attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta
    10081 actaccaacc gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg
    10141 cgtataacac agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat
    10201 ccctgaaatg tattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta
    10261 gttgtttgag cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg
    10321 aggcaggttc ttttatttac tattttcagg cctaacacca ctgcctgtgc aacatcgtgt
    10381 tccaacgaac gaattataac cacctctgga cagtgttgca attaagataa tgagatttat
    10441 aatcctgact gttgggaagt tgcgccggat cgaacccatt tcaagtcctg gctaccggac
    10501 gggtggtgcc gcaggtagtg ctctgatccg gagtgcaaca tggcgactca acaaacgtaa
    10561 accagtatcg acaggcagtg taagggtaag gtgctggtgt tgtgctcctt aataaggaga
    10621 gcggatggac acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt
    10681 ggctctgtta cggttgattg atttgttgaa gtaacgggca aattgaaaca catctaaggg
    10741 ggaataccgt tccccccttt aataaagcta tactcgtcat acttcaagtt gcatgtgctg
    10801 cgacttgaat tatctagagt atataattct atttgttggt tgaatatatt ctgtagttta
    10861 caattaaaaa ttactattac aggttcaggt gattcgcctc gtaatttttg ctcatcaata
    10921 aactcatctc ttatataaat caacgcatca tttattagcg ggtcaacatc ctctcgatca
    10981 cgttgatgaa atgttggacg tagccggaac cgttccagtc ggtagacgga acctgtaaat
    11041 aattcagagc atttatggat ctcaagtctg aaggaaaacg gatcttcagg tacagggacc
    11101 tcataatcgt aaatagcaac cagctctttt tgcacgatca tcgctttccc cgctggtaag
    11161 tcgtgtagat aacgggcagt cgcatcactg cccgctttgt tttaatggaa aatgacgtca
    11221 ggtgcgctta acgcctcact caaatcaaac acatcaagtt cggcaaaagt gacggattta
    11281 ttgtcacggt acactcgaag agaacggaag ctcattagcg ctttgctatc aacattgtag
    11341 tggttaaaac cgggtgaaaa cttaaggacc agccggtact cacccaggtc atagacaaat
    11401 gcgtcctcat aacctgactc ccagcctttg tatcgcattc ccataagttg cccaacggta
    11461 gcggatttac cttcccagcg agtcaggctg ttactggttg actcgtcagg tgtgagcagc
    11521 accgtgcggc gcgacaactg gcgaaaaggc ggcataattt cgtaatcggc aaaaagctgt
    11581 gcaaattcac tgcgc
//
LOCUS       DADMFL010000121        11555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.122, whole genome shotgun sequence.
ACCESSION   DADMFL010000121 DADMFL010000000
VERSION     DADMFL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.122"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JNZ27_003341"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNZ27_003341"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNZ27_003342"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNZ27_003342"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY8665555.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNZ27_003343"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNZ27_003343"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY8665556.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNZ27_003344"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNZ27_003344"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY8665557.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /locus_tag="JNZ27_003345"
     CDS             complement(3367..4701)
                     /locus_tag="JNZ27_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8665558.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JNZ27_003346"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JNZ27_003346"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY8665559.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNZ27_003347"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNZ27_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY8665560.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JNZ27_003348"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JNZ27_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JNZ27_003349"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JNZ27_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY8665561.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JNZ27_003350"
     CDS             9047..9826
                     /locus_tag="JNZ27_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY8665562.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JNZ27_003351"
     CDS             9816..10625
                     /locus_tag="JNZ27_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY8665563.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JNZ27_003352"
     CDS             10712..11146
                     /locus_tag="JNZ27_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8665564.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11555
                     /locus_tag="JNZ27_003353"
     CDS             11143..>11555
                     /locus_tag="JNZ27_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8665565.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2756 a         3101 c         2949 g         2749 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcacta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccga cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactcgaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaaccatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 agcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg catcc
//
LOCUS       DADMFL010000122        11459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.123, whole genome shotgun sequence.
ACCESSION   DADMFL010000122 DADMFL010000000
VERSION     DADMFL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.123"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="JNZ27_003354"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="JNZ27_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY8665566.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="JNZ27_003355"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="JNZ27_003355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY8665567.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="JNZ27_003356"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="JNZ27_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY8665568.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /locus_tag="JNZ27_003357"
     CDS             3240..4580
                     /locus_tag="JNZ27_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8665569.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /locus_tag="JNZ27_003358"
     CDS             4658..6304
                     /locus_tag="JNZ27_003358"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY8665570.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6421..7050
                     /locus_tag="JNZ27_003359"
     CDS             6421..7050
                     /locus_tag="JNZ27_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY8665571.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7189..8598)
                     /gene="melB"
                     /locus_tag="JNZ27_003360"
     CDS             complement(7189..8598)
                     /gene="melB"
                     /locus_tag="JNZ27_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY8665572.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8713..10068)
                     /gene="melA"
                     /locus_tag="JNZ27_003361"
     CDS             complement(8713..10068)
                     /gene="melA"
                     /locus_tag="JNZ27_003361"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY8665573.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10351..11259
                     /gene="melR"
                     /locus_tag="JNZ27_003362"
     CDS             10351..11259
                     /gene="melR"
                     /locus_tag="JNZ27_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY8665574.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3011 a         2939 c         2768 g         2741 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gacttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagct aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atagtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaattgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagca cctggcagca tgctgccagg tgctgggcca cctcttttgc
     6361 gattgtaatt tcatgcttgc tggtgaatag tcagtatttt ccctgacttg cgaactcacc
     6421 atgacccgca cactcaagcc gttaattctt aacaccggcg cactggcgct aacgttaatc
     6481 ctgatttata ccggcatttc ggcccatgac aaactcacct ggctgctgga agtgacaccg
     6541 gtgattatcg tcgtgccgct actacttgcc accgccagac gttacccgtt aacgctgctg
     6601 ctctatacgc tcatcttctt tcacgccatc atcctgatgg taggcgggca gtacacctac
     6661 gcgaaagtcc ccgttggttt tgaggtgcag gaatggctgg ggttgagtcg taatccgtat
     6721 gacaagctgg ggcacttttt ccaggggctg gtgcctgcac tggtggcacg agaaattctc
     6781 gttcgcggga tgtacgtgcg cggacgtaaa atggtggcgt ttctggtctg ttgcgtggcg
     6841 ctggcgataa gcgcgatgta tgaattaatc gagtggtggg cggcactggc gatgggccag
     6901 ggagcggatg attttctcgg tacccagggc gaccagtggg acacgcaatc cgatatgttc
     6961 tgcgcgctgc ttggcgcatt aacgacagtg atattgctcg ctcgctttca ttgccgccag
     7021 ctacggcgct atggcttgat aaccggataa caacgtgcac tttgttgatg ccggatgcgg
     7081 cgtgaattcc ttatccggtc tacaaaattt gcaaatccaa agcattacat taagacgtgg
     7141 cctgataaac atatcgcatc aggccatttt cgttagctat tgtccacgtc aggctttcac
     7201 atcactcact gcaccgaccg gaatatcagc atgaacaggc tcgggcggta ctttgcgata
     7261 tttatccagc agatgaatct ggatcctgcg cagcgtgtcg ccattgaggc gatagaaacg
     7321 gaagtagaga atcagcgtta ccatgaaaaa cagagtcggt agagcaatca taataaactg
     7381 catacctaat agggcttgcg tagactgttc aacattcggt acatagccaa tcatccctaa
     7441 cacaaccgca atgaaaaaag ccgcaaaggc tgaaccgccc ttcaccacca tagtctgcac
     7501 ggagtaagcg atgctttcac agcgtacgtg cagtttatat tcaccgtaat caacggtatc
     7561 tgccaccatg atgacctgta atacccagaa aagcgccgtt cccacattca gcaaaatacc
     7621 cgcaatcaca atgaggacga cgttgtgata gctcattaat gccattaaca ggagaacacc
     7681 acagcttaac accggaagaa tagatgctcc ggcccataaa atgcgtcggg ataatgattt
     7741 aactaagcgt gggaagaata ctaacgtcac caggttagca gctcccgcat acgacagata
     7801 ataggggaac aaatccgcat caccgataac atatgagaaa taatagatag caaatccggt
     7861 aataatgttg ctggctacat tataagcaag agccataccc aagaggcatg aaagctgatc
     7921 gtttttataa ataagcgcaa cgatggcttt aagtgtcaga tggcttcctt cagcagacga
     7981 ttgattgtct gacgaaaaga cttcatgcac attgcgcaga gtgatgatgg ttgaaacaat
     8041 aaaaaaggcg atcaatacca gagtgaacat ctgaaagcca aatccccgat cgccaccgcc
     8101 gacataatta acaaatggta gcgtcacacc tgccgtaaca aagcctgcca gactggcaaa
     8161 aaaacgcgga taaggaacca gttgttcgcg ttcacgttta tcgagcgtga tggttggaac
     8221 cagcgaccag aagggaatat ccataatggt gtaagtcatg ccccagagga tgtaggtcac
     8281 gcaaacaaag acaatctgag tagtaccttc aaacagatgc gcactaaaga ggagaaataa
     8341 gattacagag tttgccaacg taccgatcag gatccagggt ttgaacttac cccatcgcga
     8401 tcgcgtagca tttacaatcc atcccataat cggatcgtta atagcatccc agatcctcgc
     8461 caccagaaac aaagtaccga ccaaacccac agacagcccg acgacatcgg tgtagtaata
     8521 catgaggtac atatacacaa tgccgatcgc aaaatccttc ccgaacgctc caaatccata
     8581 actgagtttt gtagtcattg aaatgctcat agggtatcgg gtcgctgtta ccaacgcccg
     8641 ccttctgtta ttggtatcgt cagaggtgtg cgccggaata aatcccccgg cgcagtagcg
     8701 tttagtcgcg ttttaacggt gcaaccagcc tggcagccag tcgccgtggg cggcaatcag
     8761 gtcgtcaaca agagcatata tttcgtcaat gcccagcacg gcggcagtat gcgggtccat
     8821 catcgcggcg tggtaaacac ggtcgcgatt ttccgtaaga atagcttcgg tcagcagcgt
     8881 ctgtacgttg atgttggttt gcatcagggc ggccagatgc gaaggtagcg taccgacttt
     8941 ggtcggctga atgccattag catcaaccag acaggctact tccacgcaac atccttgtgg
     9001 caggttatca atcaaaccat cgttacggac gttgccgtaa atcacactcg gctcgccagt
     9061 ccagatagcg ttcatgattg tgctggcata ttcccgtgac ggtttaatat caatccggga
     9121 ggcgttttta tactcctcca gctctttatg ccagttcgcc agctgctcga cgcagcgttt
     9181 cgggtactca tccagcggta ctttataacg ctcaatcaaa tcctcacgac ctagcttaat
     9241 aaaccacggc gtatattcgg caaaatgttc tgacgattcc gtgacgaagt agcccagctt
     9301 tttgaacatt tcgtagcgca caatattctg gcagcgagta ttaccatgaa tatttggctt
     9361 cggtgcctgc cctgcttcat aagccgccag cagttccggg tagagattca cgtaactgcc
     9421 gtcggcggtt ttgcgctcca gctccaggta aaacgccata tggttgatac ctgcgcaacg
     9481 gtaacgcagc gtagccgggt cgatattgag gtcacgcgcc agctcttccg ccgttccctg
     9541 caccgaatgg cacagcccga cctgtttgat atacggatag cgggcataca tcgcccaggt
     9601 attcatcgcc attgggttaa catagttgag catggtggca tcggggcaga cttccgtcat
     9661 gttctcgcaa atttgccaca gatgcggaat ggtacgtagc gcgcgcataa taccgcccgg
     9721 ccccaacgta tcggcaatgg tttgttccag accatgccgc ttacagacct cgaaatcagt
     9781 caccgtgcaa ggttcataac cgccaatctg aaatgccacc acgacaaaat cggcatcctc
     9841 taaggcttct ttctgttggg tgtggcaggt gattttgccg ctggcccctg ctgaatccat
     9901 cagcttacgc accacaatat gcgactcttc caggcgggtg gggtcaatgt ccatcagggc
     9961 aatatgcgcc gttttcagcg cctcgcgatg gaacacatca ccaagaatat ttttaacgaa
    10021 aatcgtcgaa ccagcgccga taaatgtaat tttgggtgca gacatcatgc agatctcctg
    10081 gcttgcttga ataacttcat catggcaggc gaatctgcag aaaacctccg gcttccaggt
    10141 tgagcattcc cataaactca gatcctgcgt gaagcagcag taaatctgag tttatgggaa
    10201 taaattacct gaaaagtaga gttttcggcc tcatgatgct cggacagtcc cctcgcccct
    10261 ctgggaagag ggtgaaaacc gtgctcccac tcgcagtcat cctccctcac tcctgccata
    10321 attctgatat tctaggaaag agagccatcc atgaatacag atacgtttat gtgcagcagc
    10381 gacgaaaaac agacccgcag cccgctgtcg ctgtattcgg aataccagcg gatggagatt
    10441 gagtttcgcg caccgcatat catgcccacc agccactggc atggtcaggt cgaagtgaat
    10501 gtgcctttcg atggcgatgt ggaatacctg atcaacaatg aaaaagtgaa tatcaatcag
    10561 ggtcatatca cgctgttctg ggcctgtaca ccgcaccaac taacagatac cggaacctgt
    10621 cagagcatgg cgatttttaa tctgccgatg catctgtttc tctcctggcc gctggataaa
    10681 gacctgatta accacgtcac tcacggcatg gtgatcaaat cacttgccac acagcaactt
    10741 agcccgtttg aagtacgccg ctggcagcag gaattaaaca gtccgaatga gcaaattcgc
    10801 cagctcgcca ttgatgaaat tggcctgatg ctcaagcgat ttagcctctc tggctgggaa
    10861 ccgattctgg tcaataaaac ctcgcgcaca cacaaaaaca gcgtctcgcg ccatgcgcaa
    10921 ttttatgtta gccagatgct gggctttatt gccgaaaact atgatcaggc gctgaccatc
    10981 aacgatgtgg ctgagcacgt caaacttaac gccaactatg caatggggat attccagcgg
    11041 gttatgcaat tgacgatgaa acagtacatt accgcgatgc gcatcaacca cgttcgcgcg
    11101 ttactgagcg ataccgataa aagtattctc gatattgccc tgacggcagg ctttcgttca
    11161 agtagtcgtt tttacagcac gttcggcaaa tatgtcggca tgtcgccgca acaataccgc
    11221 aaacttagcc aacagcgccg ccagacgttt cccggctaaa gacgatatca gtatcagcca
    11281 aaaaaatagt ttagccgtcg ataataagca gtgaaaataa tttaaaataa tcacataacg
    11341 tattgtttat aaaactaaat atttcaaaaa tgtttgtttt tcacgcgctt tacagcccga
    11401 aaaggccgga agatacttgc ccgcaacgaa gattccggta atgactccaa cttactgat
//
LOCUS       DADMFL010000123        11242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.124, whole genome shotgun sequence.
ACCESSION   DADMFL010000123 DADMFL010000000
VERSION     DADMFL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.124"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            143..434
                     /locus_tag="JNZ27_003363"
                     /pseudo
     CDS             143..434
                     /locus_tag="JNZ27_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
     gene            complement(554..733)
                     /locus_tag="JNZ27_003364"
     CDS             complement(554..733)
                     /locus_tag="JNZ27_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY8665575.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            807..2168
                     /gene="pabB"
                     /locus_tag="JNZ27_003365"
     CDS             807..2168
                     /gene="pabB"
                     /locus_tag="JNZ27_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY8665576.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2172..2750
                     /locus_tag="JNZ27_003366"
     CDS             2172..2750
                     /locus_tag="JNZ27_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY8665577.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2934..4298
                     /gene="sdaA"
                     /locus_tag="JNZ27_003367"
     CDS             2934..4298
                     /gene="sdaA"
                     /locus_tag="JNZ27_003367"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8665578.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4429..6027
                     /locus_tag="JNZ27_003368"
     CDS             4429..6027
                     /locus_tag="JNZ27_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8665579.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6031..7581)
                     /gene="yoaE"
                     /locus_tag="JNZ27_003369"
     CDS             complement(6031..7581)
                     /gene="yoaE"
                     /locus_tag="JNZ27_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY8665580.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8044..9015
                     /gene="manX"
                     /locus_tag="JNZ27_003370"
     CDS             8044..9015
                     /gene="manX"
                     /locus_tag="JNZ27_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY8665581.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9078..9878
                     /locus_tag="JNZ27_003371"
     CDS             9078..9878
                     /locus_tag="JNZ27_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8665582.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9891..10742
                     /locus_tag="JNZ27_003372"
     CDS             9891..10742
                     /locus_tag="JNZ27_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY8665583.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10797..>11242
                     /locus_tag="JNZ27_003373"
     CDS             10797..>11242
                     /locus_tag="JNZ27_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY8665584.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNINDLEKIYKLLV"
BASE COUNT         2767 a         2837 c         2833 g         2805 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccaactc gtgccatatc
       61 gttataatcc ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat
      121 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
      181 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat
      241 atttaaaaga actgggagta cccgcgagtg ccgctgatat tacggcgcga gctgaccagg
      301 aaggctggaa ccctgggttc acggaaaaaa tggttggatg ggcaaaaaaa atggagacag
      361 gtgaacgctc tgtgattaaa aatcctgaat acttttcaac atatatgcag gaagaactaa
      421 aagcactggt ctgagttaaa tttatatcag cataaatggg tcaggacgct tttaatcaca
      481 tataaaaagc gtcctttttc tcacaatcaa atgatcatcg gcttctataa cgaaatcgat
      541 acggattagt gtgttactcg tcttcatcct caaaacgcgc cacgattagc tcgccggagt
      601 ggttggcacg cagctcttct gccaccagcg cgattgcctg gccgctactc atcccttgtg
      661 ccatcagttc ctggatccgc tcgacagctt tttgctgctg ttcatgggtg agtgaaggta
      721 aacctgcaaa cattgttaac tcctgctaaa ttgttggcgc taattatttc atgctacccg
      781 gcacatagcc agtagagtca ggactgatga agacgttatc tcccgctgtg attactttac
      841 cctggcgtca ggacgccgct gaattttatt tctcccgctt aagccacctg ccgtgggcga
      901 tgcttttaca ctccggctat gccgatcatc cgtatagccg ctttgatatt gtggtcgccg
      961 atccgatttg cactttaacc actttaggta aagaaaccgt tgttagtgaa agcgaaaaac
     1021 gcacaacgac cactgatgac ccgctacagg tgctccagca ggtgctggat cgcgcagaca
     1081 ttcgcccaac gcataacgaa gatttgccat ttcagggcgg cgcactgggg ttgtttggct
     1141 acgatctggg ccgccgtttt gagtcactgc cagaaattgc agaacaagat atcgttctgc
     1201 cggatatggc agtgggtatc tacgattggg cgctcattgt cgaccaccag cgtcatacag
     1261 tttctttgct gagtcataat gatgtcaatg cccgtcgggc ctggctggaa agccagcaat
     1321 tctcgccgca ggaagatttc acgctcactt ccgactggca atccaatatg acccacgagc
     1381 agtacggcga aaaatttcgc caggtacagg aatatctgca cagcggtgat tgctatcagg
     1441 tgaatctcgc ccagcgtttt catgcgacct attctggcga tgaatggcag gcattccttc
     1501 agcttaatca ggccaaccgc gcgccattta gcgctttttt acgtcttgaa cagggtgcaa
     1561 ttttaagcct ttcgccagag cggtttattc tttgtgataa tagtgaaatc cagacccgcc
     1621 cgattaaagg cacgctacca cgcctgcccg atcctcagga agatagcaaa caagcagaaa
     1681 aactggcgaa ctcagcgaaa gatcgtgccg aaaatctgat gattgtcgat ttaatgcgta
     1741 atgatatcgg tcgtgttgcc gtagccggtt cggtaaaagt accagagctc ttcgtggtgg
     1801 aacccttccc tgccgtgcat catctggtca gcactataac ggcgcgacta ccagaacagt
     1861 tacacgccag cgatctgctg cgcgcagctt ttcctggtgg ctcaataacc ggggctccga
     1921 aagtacgggc tatggaaatt atcgacgaac tggaaccgca gcgacgtaat gcctggtgcg
     1981 gcagcattgg ctatttgagc ttttgcggca acatggatac cagcattact atccgcacgc
     2041 tgactgccat taacggacaa atatactgct ctgcgggcgg tggaattgtc gccgatagcc
     2101 aggaagaagc ggaatatcag gaaacttttg ataaagttaa taagatatta cgccaactgg
     2161 agaagtaaaa cgtggaatac cgtagcctga cgcttgatga ttttttatct cgctttcaac
     2221 ttttgcgccc gcaaattaac cgggaaaccc taaatcatcg tcaggctgcc gtgttaatcc
     2281 ccatcgtccg tcgaccacaa ccggggttgt tgctgactca gcgttcaatt catctgcgta
     2341 aacacgctgg acaagtggca ttccctggag gtgcagtcga tgacacggac gcatcagtta
     2401 tcgccgccgc gctgcgcgaa gctgaagaag aggtcgctat accgccttcc gccgttgaag
     2461 ttatcggcgt gctgccgccc gtcgatagcg taactggcta tcaggtaacc ccagtggtcg
     2521 gcattatccc gcccgatctg ccgtatcgcg ccagtgaaga tgaagtctcg gcggtgtttg
     2581 aaatgccgct cgcccaggca ttacatctgg gtcgttatca ccctttagat atctaccgcc
     2641 gtggcgattc acatcgggta tggctgtcct ggtacgaaca gtattttgta tggggaatga
     2701 ccgcaggcat aattcgtgag ctggcgctgc aaattggtgt gaacccctga ctatacttat
     2761 ctttacatct acaaaacact acttgagaca atcatcgcaa tattagttaa atcgcggttt
     2821 ttgattagtt taattcatgt gaatagttaa gccagtcgcc gcgttccctc ttacactatg
     2881 cgctgttatt agttcgttac tggaagtcca gtcaccttgt caggagtatt atcgtgatta
     2941 gtctattcga catgtttaag gtggggattg gtccctcatc ttcccatacc gtagggccta
     3001 tgaaggcggg taaacagttc gtcgatgatc tggtcgaaaa aggcttactg gatagcgtta
     3061 ctcgcgttgc cgtggacgtt tatggttcac tgtcgctgac gggtaaaggc caccacaccg
     3121 atatcgccat tattatgggt cttgcaggta acgaacctgc caccgtggat atcgacagta
     3181 ttcccggttt tattcgcgac gtaaaagagc gcgaacgtct gctgctggca cagggacggc
     3241 atgaagtgga tttcccgcgc gacaacggga tgcgttttca taacggcaac ctgccgctgc
     3301 atgaaaacgg tatgcaaatc cacgcctata acggcgatga agtcgtctac agcaaaactt
     3361 attattccat cggcggcggt tttatcgtcg atgaagaaca ctttggtcag gatgctgcca
     3421 acgaagtgag cgtgccgtat ccgttcaaat ctgccaccga actgctcgcg tactgtaatg
     3481 aaaccggcta ttcgctgtct ggtctcgcta tgcagaacga actggcgctg cacagcaaga
     3541 aagagatcga cgagtatttc gcgcatgtct ggcaaaccat gcaggcatgt atcgatcgcg
     3601 ggatgaacac cgaaggtgta ctgccaggcc cgctgcgcgt gccacgtcgt gcgtctgccc
     3661 tgcgccggat gttagtttcc agcgataaac tgtctaacga tccgatgaat gtcattgact
     3721 gggtaaacat gtttgcgctg gcagttaacg aagaaaacgc cgccggtggt cgtgtggtaa
     3781 ctgcgccaac caacggtgcc tgcggtatcg ttccggcagt gctggcttac tatgaccact
     3841 ttattgaatc ggtcagccca gacatctata cccgttactt tatggcagcg ggcgcgattg
     3901 gtgcattgta taaaatgaac gcctctattt ccggtgcgga agttggttgc cagggcgaag
     3961 tgggtgttgc ctgttcaatg gctgctgcgg gtcttgcaga actgctgggc ggtagcccgg
     4021 aacaggtttg cgtggcggcg gaaattggca tggaacacaa ccttggttta acctgcgacc
     4081 cggttgcagg gcaggttcag gtgccgtgca ttgagcgtaa tgccattgcc tctgtgaagg
     4141 cgattaacgc cgcacggatg gctctgcgcc gcaccagtgc accgcgcgtc tcgctggata
     4201 aggtcatcga aacgatgtac gaaaccggta aggacatgaa cgccaaatac cgcgaaacct
     4261 cacgcggtgg tctggcaatc aaagtccagt gtgactaata cttcttactc gcccatctgc
     4321 aacggatggg cgaatttata cccgctttct cgtctgctgt aatattcccc actacacttc
     4381 cactgttgcg tcaggcgttt gtcgccatac gcttacaggg tggcccgcat gcaaaaagca
     4441 caacggatca ttaaaaccta tcgccgtaat cgaatgattg tttgtacgat ttgcgcactc
     4501 gttacgctcg cttcgaccct gagcgtgcga tttatttcac agcgtaactt aaatcaacaa
     4561 cgggtagtac aattcgccaa tcacgctgta gaggaattag ataaagtact gcttccccta
     4621 caggcaggta gcgaagtctt gcttccgctg attggtctgc cctgctctgt cgcccatttg
     4681 ccattacgta aacaggcggc aaaactccaa actgtgcgat ccattggcct ggtgcaagac
     4741 ggcacacttt attgctccag catttttggt tatcgcaatg tgcccgtcgt ggacattctg
     4801 gctgaacttc ctgcaccgca accactttta cgcctgacga tcgaccgtgc cctgattaaa
     4861 ggcagtccgg ttttgattca atggacgcct gcagcaggca gtagcaatgc tggggtcata
     4921 gagatgatta acatcgactt actgacggca atgctccttg agccacaact gccgcaaatc
     4981 agtagcgcca gcctgacggt ggacaaacgg catttgctct atggtaatgg gctggtagat
     5041 tcccttccgc aacctgaaga caatgaaaac tatcaggttt cttcgcaaag ctttcctttt
     5101 accattaacg taaatggtcc ggaggctacg gcgctggcat ggcactatct tccaacacaa
     5161 ttaccgctgg cggtgctgct aagtttactg gtgggctata tcgcctggct ggcgaccgct
     5221 taccggatga gcttttcccg cgaaattaat ctgggcctgg cgcaacatga gttcgaattg
     5281 ttctgtcagc ctttgcttaa tgcgcgcagc cagcaatgta ttggtgtaga gattttgctg
     5341 cgctggaata atccgcgtca gggctggatt tcaccggatg tgtttattcc tatcgcggaa
     5401 gaacatcatt taattgtgcc actgacccgc tatgtgatgg cagaaaccat tcgtcagcgc
     5461 catgttttcc cgatgagtag tcagtttcat gttggcatta acgtcgcacc cagccatttt
     5521 cgccgtggtg tgctgataaa agatctcaat cagtactggt ttagcgctca cccgattcag
     5581 caactgatcc tcgaaatcac cgaacgcgat gccttactgg atgttgatta tcggattgcc
     5641 cgcgagctgc atcgtaaaaa cgtcaaactg gcgattgatg acttcggcac cggcaacagc
     5701 tcgttttcct ggcttgaaac attacgtcct gacgtgctga aaattgataa gtcatttacc
     5761 gcagctatag gttctgacgc ggttaactcg acggtgaccg atatcatcat cgcgctgggg
     5821 caaagactga atattgaact ggtggcggag ggcgtggaaa cgcaagaaca ggcgaagtat
     5881 ttgcgccgtc atggcgtgca tattttgcaa gggtatttgt acgcacagcc gatgccgcta
     5941 cgtgattttc ccaaatggct ggcgggcagc caaccgccgc ccgcccggca taatggacat
     6001 atcacgccca ttatgtcgtt acgttaacgc ttactcatct tcatcgtgcg ccggttgctc
     6061 tttaacaatg cgaaccagat caacacgata atcattggct tcaaggatgg tgatatgcag
     6121 tggccctaca tcaatcacat cgcccacacg gggaatgtga ccatttgccg agatcacgag
     6181 gcccgcgacc gtcgcgatat catcgtcatc ggcaaggtgc tcaacatcaa gcgcctgctg
     6241 caaggcatgc aaatctgtac cgccttttac cagccagccg ttaccatcgg taatgatttc
     6301 cggcgtttcg tcagcgtccg ggaattcacc cgcaatggct tccagcacat ccagcggcgt
     6361 gaccaagcct tgtaccacac caaactcgtt ggtcacgata acaaagctcc cgcgagcgcg
     6421 acgcagcacg cccagcaggt tgatcggatc gagggtttcc gggacgataa tcgccggaga
     6481 cgccgaagca atcgccgcca catcaacgcc ctcttccagc gccaccagca gttctttagc
     6541 acgtacgata ccgatgattt catccagttc accgcgacat accgggaaca gactgtgcgg
     6601 tgaagagagc agttgctcgc ggatttcatc gaccccgaga ttagcgtcaa cccagcttat
     6661 ttcaccgcgc ggcgtcatga tcccgcgcag agaacgcgac gccagcgtca gtacgccgtt
     6721 aatcatgtac cgttcttctt cggcaaatgc accttccggg atcggcatcg ggttatcggc
     6781 atcgtgctga acattggcct gacgtttccc gcccatcaaa cgcaggatgg catcggcagt
     6841 acgcgctcgc agcggcaaag tcgactggtg acgaataaag ttgcgacgcg caatctggtt
     6901 aaacacttcg ataatgatcg agaagccaat cgcggcatac aggtaacctt tcggaatgtg
     6961 gaaaccgaaa ccttctgcca ccagactcag accaatcatt aacaggaagc tcagacagag
     7021 caccaccacc gtggggtgct ggttaacgaa tcgcgtcagc ggtttcgatg ccagcaacat
     7081 aaccgccatc gcaatcacta ccgccgccat catcaccggc agatggttaa ccatccctac
     7141 tgcagtaatt accgcatcca acgagaagac ggcgtcaagg atgacaatct gtgtgacgac
     7201 cacccagaaa ctggcgtagc ctttaccgtg gccggaatca tgatcgcggt tttccagccg
     7261 ttcatgcagt tcggttgttg ctttgaacag caagaatatc cccccgaaca acataatcag
     7321 atcgcgtccg gagaaggaga aatccatgac ggtaaatagc ggtttggtca gcgtgaccat
     7381 ccatgaaatc agcgacagca gccccagacg cataatcagc gccagtgata accccagcaa
     7441 acgcgcttta tcgcgttgtt ttggcggcag tttgtcagca agaatggcga tgaagaccag
     7501 gttatcgata cccagcacaa tttcgagaac aacaagcgtg agtagccccg cccaaattga
     7561 ggggtccatt aagaattcca tgacaagctc ctgcttaagg aatagctatt cgacgccaga
     7621 aataatgcag gcgtaacgac aaaatgcaaa cgaaaggtgc ggcatagagt gccagaaagg
     7681 caggcgttaa aaggcctgat gctgaaatga cgtcggtgac gatccatact gcgggctact
     7741 gccctatact ccatggttgt taaacgggag ttaaacatat cagagacgcc tctgatttgg
     7801 caaagattta ccttcctttg caaacgaatg tgacaaggat attttacctt tcgaaatttc
     7861 tgataatcga aagttaaatt acggatcttc atcacataaa ataatttttt cgatatctaa
     7921 aataaatcgc gaaacgcagg ggtttttggt tgtagccctt atctgaatcg attcgattgt
     7981 ggacgacgat tcaaaaatac atctggcacg ttgaggtgtt aacgataata aaggaggtag
     8041 caagtgacca ttgctattgt tataggcaca catggttggg ctgcagagca gttgcttaaa
     8101 acggcagaaa tgctgttagg cgagcaggaa aacgtcggct ggatcgattt cgttccaggt
     8161 gaaaatgccg aaacgctgat tgaaaaatac aacgctcagt tggcaaaact cgacaccact
     8221 aaaggcgtgc tgtttctcgt tgatacatgg ggaggcagcc cgttcaatgc tgccagccgc
     8281 attgtcgtcg acaaagagca ttatgaagtc attgcaggcg ttaacattcc aatgctcgtg
     8341 gaaacgttaa tggcccgtga tgatgacccg agctttgatg aactggttgc gctggcagta
     8401 gaaacaggcc gtgaaggcgt gaaagcactg aaagccaaac cggttgaaaa agccgcgcca
     8461 gcacccgctg ccgcagcacc aaaagcggct ccaactccgg caaaaccaat ggggccaaac
     8521 gactacatgg ttattggcct tgcgcgtatc gacgaccgtc tgattcacgg tcaggtcgcc
     8581 acccgctgga ccaaagaaac caatgtctcc cgtattattg ttgttagtga tgaagtggct
     8641 gcggataccg ttcgtaagac actgctcacc caggttgcac ctccgggcgt aacagcacac
     8701 gtagttgatg ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc tggcgaacgc
     8761 gtaatgctgt tatttaccaa cccaacagat gtagagcgtc tcgttgaagg cggcgtgaaa
     8821 atcacctctg ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca ggtgaataac
     8881 gcggtttcgg ttgatgaaaa agatatcgag gcgttcaaaa aactgaatgc acgcggtatt
     8941 gagctggaag tccgtaaggt ttccaccgat ccgaaactga aaatgatgga tctgatcagc
     9001 aaaatcgata agtaacgtat tgtgttgatt atcattcagt tttcacactt aagtcttacg
     9061 taaacaggag aagtacaatg gagattacca ctcttcaaat tgtgctggta tttatcgtag
     9121 cctgtatcgc aggtatggga tcaatcctcg atgaatttca gtttcaccgt ccgctaatcg
     9181 cgtgtaccct ggtgggtatc gttcttgggg atatgaaaac cggtattatt atcggtggta
     9241 cgctggaaat gatcgcgctg ggctggatga acatcggtgc tgcagttgcg cctgacgccg
     9301 ctctggcttc tatcatttct accattctgg ttatcgcagg tcatcagagc attggtgcag
     9361 gtatcgcact ggcaatccct ctggccgctg cgggccaggt actgaccatc atcgttcgta
     9421 ctattaccgt tgctttccag cacgctgcgg ataaggctgc tgataacggc aacctgacag
     9481 cgatttcctg gatccacgtt tcttctctgt tcctgcaagc aatgcgtgtg gctattccgg
     9541 ccgtcatcgt tgcgctgtct gttggtacca gcgaagtaca gaacatgctg aatgcgattc
     9601 cggaagtggt gaccaatggt ctgaatatcg ccgggggcat gatcgtggtg gttggttatg
     9661 cgatggttat caacatgatg cgtgctggct acctgatgcc gttcttctac ctcggcttcg
     9721 taaccgcagc attcaccaac tttaacctgg ttgctctggg tgtgattggt actgttatgg
     9781 cagtgctcta catccaactt agcccgaaat acaaccgcgt agccggtgcg cctgctcagg
     9841 cagctggtaa caacgatctc gataacgaac tggactaaca ggtgagcgaa atggttgata
     9901 caactcaaac taccaccgag aaaaaactca ctcaaagtga tattcgtggc gtattcctgc
     9961 gttctaacct cttccagggt tcatggaact tcgaacgtat gcaggcactg ggtttctgct
    10021 tctctatggt accggcaatt cgtcgcctct accctgagaa caacgaagct cgtaaacaag
    10081 ctattcgccg tcacctggag ttctttaaca cccagccgtt cgtggctgca ccgattctcg
    10141 gcgtaaccct ggcgctggaa gaacagcgtg ctaatggcgc agagatcgat gacggtgcta
    10201 tcaacggtat caaagtcggt ttgatggggc cgctggctgg tgtaggcgac ccgatcttct
    10261 ggggaaccgt acgtccggta tttgcagcac tgggtgccgg tatcgcgatg agcggcagcc
    10321 tgttaggtcc gctgctgttc ttcatcctgt ttaacctggt gcgtctggca acccgttact
    10381 acggcgtagc gtatggttac tccaaaggta tcgatatcgt taaagatatg ggtggtggct
    10441 tcctgcaaaa actgacggaa ggggcgtcta tcctcggcct gtttgtcatg ggagcattgg
    10501 ttaacaagtg gacacatgtc aacatcccgc tggtggtttc gaccatcacc gggcaggatg
    10561 gacagacccg tgtcaccacc gtgcaaacta ttcttgacca gttaatgcca ggccttgtac
    10621 cactgctgct gacatttgct tgtatgtggc tgctgcgcaa aaaagttaac ccgctgtgga
    10681 tcatcgttgg cttcttcgtc atcggtatcg ctggttacgc ttgcggcctg ctgggactgt
    10741 aagactgttg tacactaccg gggccttttg gccccgtttt tttatctgga ggattaatga
    10801 caatcacgga cctggtactg attcttttca tcgccgcact cctggccttc gcgatctacg
    10861 atcagttcat catgccccgc cgtaacggcc ccaccctgct ggcaattcct ttgctccggc
    10921 gtggtcgcat cgatagcgtt atcttcgtcg gattgattgt cattcttatc tataacaacg
    10981 tcacgaatca tggcgcgcta ataacaacat ggttattaag tgcactggct ctgatgggtt
    11041 tttatatatt ctggatccgc attccgaaga tcatctttaa acaaaaaggt tttttcttcg
    11101 ccaatgtctg gattgaatat agccgaatca aagcgatgaa cttgtcggaa gatggcgtgt
    11161 tggtgatgca attagaacag cgtcggctgt taatccgcgt tcgaaatatc aacgatctgg
    11221 aaaaaattta taagcttctc gt
//
LOCUS       DADMFL010000124        11119 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.125, whole genome shotgun sequence.
ACCESSION   DADMFL010000124 DADMFL010000000
VERSION     DADMFL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.125"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            216..2946
                     /gene="malT"
                     /locus_tag="JNZ27_003374"
                     /pseudo
     CDS             216..2946
                     /gene="malT"
                     /locus_tag="JNZ27_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(2989..3795)
                     /gene="rtcA"
                     /locus_tag="JNZ27_003375"
     CDS             complement(2989..3795)
                     /gene="rtcA"
                     /locus_tag="JNZ27_003375"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY8665585.1"
                     /translation="MGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADG
                     PSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVS
                     PVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSLHEQNIHNLPRDQ
                     GPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASPAAVGEYLADQL
                     VLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTRVSIE"
     gene            complement(3870..5096)
                     /locus_tag="JNZ27_003376"
     CDS             complement(3870..5096)
                     /locus_tag="JNZ27_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY8665586.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5285..6883
                     /locus_tag="JNZ27_003377"
     CDS             5285..6883
                     /locus_tag="JNZ27_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY8665587.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADKQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEHIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6865..7623)
                     /locus_tag="JNZ27_003378"
     CDS             complement(6865..7623)
                     /locus_tag="JNZ27_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY8665588.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7640..8470)
                     /gene="glpG"
                     /locus_tag="JNZ27_003379"
     CDS             complement(7640..8470)
                     /gene="glpG"
                     /locus_tag="JNZ27_003379"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY8665589.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8515..8841)
                     /gene="glpE"
                     /locus_tag="JNZ27_003380"
     CDS             complement(8515..8841)
                     /gene="glpE"
                     /locus_tag="JNZ27_003380"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY8665590.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGKSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9031..10536
                     /gene="glpD"
                     /locus_tag="JNZ27_003381"
     CDS             9031..10536
                     /gene="glpD"
                     /locus_tag="JNZ27_003381"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY8665591.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10805..11035)
                     /locus_tag="JNZ27_003382"
                     /pseudo
     CDS             complement(10805..11035)
                     /locus_tag="JNZ27_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2684 a         3042 c         2851 g         2542 t
ORIGIN      
        1 tgatgaacat aagggaaacc agtattcacg ctggatcagc gtcgttttag gtgagttgtt
       61 aataaagatt tggaattgtg acacagtgca aattcagaca cataaaaaaa cgtcatcgct
      121 tgcattagaa aggtttctgg ccgaccttat aaccattaat tacgaagcgc aaaaaaaata
      181 atatttcctc attttccaca gtgaagtgat taactatgct gattccgtca aaactaagtc
      241 gtccggttcg actcgaccat accgtggttc gtgagcgcct gctggctaaa ctttccggcg
      301 cgaacaactt ccggctggcg ctgatcacga gtcctgcggg ctacggaaag accaccctca
      361 tttcccagtg ggcggcaggc aaaaacgata tcggctggta ctcgctggat gaaggtgata
      421 accagcaaga gcgtttcgcc agctatctca ttgccgccgt gcagcaggca accaacggtc
      481 actgtgcgat atgtgagacg atggcgcaaa aacggcaata tgccagcctg acgtcactct
      541 tcgcccagct tttcattgag ctggcggaat ggcatagccc actttatctg gtcatcgatg
      601 actatcatct gatcactaat ccagtgatcc acgagtcaat gcgcttcttt attcgccatc
      661 aaccagaaaa tctcaccttg gtggtgttgt cacgcaacct tccgcaactg ggcattgcca
      721 atctgcgtgt tcgtgaccaa ctgctggaaa ttggtagtca gcaactggca tttacccatc
      781 aggaagcgaa gcagtttttt gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca
      841 gtcggatttg cgatgacgtt tccggttggg cgacggcact acagctaatc gccctctccg
      901 cccggcagaa tacccactca gcccataagt cggcacgccg cctggcggga atcaatgcca
      961 gccatctttc ggattatctg gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc
     1021 attttctgtt gaaaagcgcc attttgcgct caatgaacga tgccctcatc acccgtgtga
     1081 ccggcgaaga aaacgggcaa atgcgtctcg aagagattga gcgtcagggg ctgtttttac
     1141 agcggatgga tgataccggc gagtggttct gctatcaccc gctgtttggc aacttcctgc
     1201 gccagcgctg ccagtgggaa ctggcggcgg agctgccagt gggaactggc ggcggagctg
     1261 ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc cagcgaagcg
     1321 attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac
     1381 gcctggaatt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg
     1441 ccgtgggaca gtttgctgga aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa
     1501 agccaacatc gctatggcga agttaacacc ctgctagccc gtgctgaaca tgaaatcaag
     1561 gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt
     1621 aacgatggta atccggatga agcggaacgg ctggcaaaac tggcactgga agagctgccg
     1681 ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc
     1741 aaaggcgaat tgacccgctc actggcgcta gtgcagcaaa ccgaacagat agcacgccag
     1801 cacgacgtct ggcactacgc cttatggagt ttaattcagc aaagtgaaat tctgtttgcc
     1861 caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct gatcaacgag
     1921 cagcatctgg aacagctgcc aatgcatgag tttctggtgc gcatccgagc acaactgtta
     1981 tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg
     2041 tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg
     2101 gcccgtggtg atttagataa cgcccgtagc cagttgaacc gtctggaaaa cctgctgggg
     2161 aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg
     2221 caaatgaccg gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag
     2281 tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg
     2341 ctgggcgagt ttgaacctgc ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt
     2401 ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact gtactggcag
     2461 gccggacgta aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc
     2521 accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag
     2581 ctgattcaac ttaatacgct gccggaactg gaacagcatc gcgcgcagcg tattctgcga
     2641 gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt
     2701 ctgctaaatc atccggaagt gccagaacta atccgcacca gcccgttgac ccaacgtgaa
     2761 tggcaggtcc tggggctgat ctactccggt tacagcaatg aacaaattgc gggcgaactg
     2821 gaagtggcgg caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggcgtc
     2881 gcccaccgtc aggctgccgt gcagcacgcg cagaaactgc tgaagatgat gggttatggg
     2941 gtgtgagttt agccggataa tgcattggat ccggtttaca tctttacatc attcaatact
     3001 cacccgcgtt acgccatctg tttctatcaa accaaaccgc accggcaaga aacgctccac
     3061 caccgcgata ttggtcagca gatggcatga aggatgggcg accgtaaatt ctcccgcgcc
     3121 cgccagcgcc atcggcagta ccagttggtc ggcgagatat tcccccaccg ccgctgggct
     3181 tgccaggtag cgtttcacct ctttcactaa ctgcgcggct accacctcgg cactgacgcg
     3241 cttttcaccg acgacgaaaa agcgttcagt gatattttca ctttcgactt ccagcgagac
     3301 ggtattaccc ggcccctggt cgcgcggcag gttatgaata ttctgttcat gcagggaaaa
     3361 aatccccgcc agtgtagcga tttcacgctc agcaacatgg cgcggcacac cagctaatag
     3421 aacttctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgctaaacga
     3481 tgccacgggc gagacttccg ttgccaccac accgcctccg gcaggataaa aaccgtgacg
     3541 taacagcgtg gtttgctgat gaattcctat tttcgccagc agcggctcca gcacccggcg
     3601 gataaaatcc gcaggcggcg ccgacgggtt atcggtgccg ccgctcactt caacacgcga
     3661 aggtccatcg gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg tacaacttcc
     3721 ggcgctaccg atagcaaagc ggtaatcgcc gccgcgcacg gtgccaggcc ggaagaccag
     3781 acgctgcgat cccagctcag cgccgagcgc aggatctgcc cgccgccttc gccctgtgcg
     3841 ccatccagcg caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg
     3901 gtgtagataa cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac
     3961 gccatcggga tttcgtcgat cacttcggca tctttacggc attccacatg cgcggtggca
     4021 cgaatttggt cttccacgct gaacagtttt ttcgctttag ttcggctcat tacccgcccg
     4081 gcaccgtggc tgcacgaaca aaacgactct tcatttccca gcccacggac gataaagctt
     4141 tttgctccca tcgaaccggg aataattcca tattgaccag cacgcgcaga caccgcacct
     4201 ttacgcgtca cgtagatctc ttcaccaaag tgctgttctt tttgcacata gttgtggtga
     4261 cagttgatct cttccatcgc cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc
     4321 tgcaacgccg ttaccacatt ttccatcatc gcatcgcggt taaggctggc gaaaagctgc
     4381 gcccaggcca cggctcttag gtaatcatca aagtattccg taccttccat aaagtacgcc
     4441 agatcacgcg acggcaacgt ctcaagttgg tcctgcatct ctttttgcgc cagatcgata
     4501 aagtaagtcc cgatggcgtt accaattcca cgtgaaccgg agtgcagcat aatccacacc
     4561 tgctccgact catcaaggca gatttcaata aagtggttac ccgttcccag cgttcccagg
     4621 tgtttatagt tgttagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg
     4681 gcctccagct cagcccattt cgcatcgacg ttaaaaggcg ggttttccca tgcgccttta
     4741 tcacgtttac aacgcccggt agtacgcccg tgtggtacgg ccgtttcaat cgcttgacgc
     4801 agctccgcca ggttttcagg caggtctgcc gccgttaacg cggtacgcag cgcgttcatt
     4861 ccacagccaa tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg
     4921 ctaccaatgg tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa
     4981 ataaacggca tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg
     5041 cctttggtcc acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg
     5101 tttttctctt ttcgttgttt gcggtcctga taattgcaac cgtcgtgcca gaaaattaaa
     5161 taagcagatt aattttttaa tttattgttt ctaaaaagat tatatcttta tgtccgtaaa
     5221 cggagatttc ccgcaaagcc gatttaccga taatgaaata tcgcctttta taaggatatc
     5281 taagatgcgt aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg
     5341 cggcagtcag cgctggtcaa aatggcgtcc gtcactctgt atatgccagc aagaatcgtt
     5401 ggtcatcgat cgactggaat tattgcacga tgcccgctcg cgctcgctat ttgaaacgct
     5461 taaacgcgat atcgccagcg tttcgccaga aacagaagtg gtgggcgttg agattgaact
     5521 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
     5581 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
     5641 gcagatttgc tggtttctgc tggcggaagc gcgttatcta cccgcccggt tgatacaatc
     5701 ttcaactccg cgcaaaaaag aacagccgcg cggcccaggt gaagtgacga ttatcgatct
     5761 cgatttaagc cgttataacg ccatcgccag ccgctttgcc gaggaacggc agcaaacgct
     5821 cgatttcctc aaatccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca
     5881 gatcgaaaaa gtggcgatta aatcccgcgc gccgattctg cttaacggcc cgaccggcgc
     5941 aggtaagtca tttctggcgc gacgcatctt cgagttaaaa caggcacggc atcagtttag
     6001 cggcgcgttt gtggaagtga actgcgccac cctgcgcggc gataccgcga tgtcgacgct
     6061 gtttggtcat gtgaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gattattacg
     6121 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacaaaca
     6181 ggcaatgctg ctgaaagcta ttgaagagaa aaccttttac ccgtttggca gcgatcgcca
     6241 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc
     6301 cgaaggcaaa ttccgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc
     6361 gggtctacgc cagcgccagg aacatattga accgaacctg gattatgaag tggagcgcca
     6421 cgccacactc actggtgaca gcgtgcgttt taacaccgaa gcgcggcgtg cctggctggc
     6481 ctttgcaaca tctccccagg ctacatggcg cggtaacttt cgcgaacttt ctgccagcgt
     6541 cacgcggatg gccacctttg ccactagcgg acgcatcact ctggacgtgg tggaagatga
     6601 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
     6661 gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc
     6721 tatctgccgc caggcaaagt cgctttctgc tgccggacga cagctttttg atgtttcgcg
     6781 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
     6841 tctgacgtgg gaggccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag
     6901 cacctgcatc acgcttactg gcggcggggc gtcggtgtag acggcatcaa ccatgctgat
     6961 gctccccata ttgaccatcg cattacggcc aaattttgaa tggtcaacca ccagcataac
     7021 gtggcgcgag ttctcaataa tggcgcgctt ggtacgaact tcgtggtaat cgaactccag
     7081 cagcgagccg tcgctgtcga tgccgctgat ccccagaatg ccgaaatcaa ggcggaactg
     7141 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctac gtaattcgcc
     7201 accggcgaga ataatgcgga aatcttcttt taccatcaac gtgttagcaa cgttgagatt
     7261 gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt
     7321 ggtgccgata tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc
     7381 gatgcgctct ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga
     7441 actggaaggc agcgccgcgc cgccatgatg gcgcaggatc aggttttgct ctgccagctc
     7501 gttgaggtcg cggcgaatag tctgcgggct gacggagaag tgctccacca gttcctcggt
     7561 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
     7621 catttataaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
     7681 tcgctaaacc cacggctaat ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
     7741 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
     7801 aaatgccact ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca
     7861 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta
     7921 acagggcgct gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg
     7981 cgccgccgag ataccaccac caaagcaggt taaagaggat atgcatcagc gagaagtgca
     8041 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg
     8101 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc
     8161 aggcgatcat catcacccag gtcaccggac ctgcgcgttc acgcaaggca gcaaagaaag
     8221 gataacggcg ataatgcagg ccactgccgg tatggcctgc cagccagctg gccgccagat
     8281 aacgcggatc tgccgggttt tcgagaaaac gcgccagctc cgcccgcacg cgctcggcct
     8341 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
     8401 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
     8461 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
     8521 gccgtacgcc acctctgcgg gaaactgtcg ctgccaggct tcaaagccgc cgtcaatgct
     8581 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcccctttgc tgctcttgcc
     8641 gtgataacac atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag
     8701 cgtgtcgttg gttaaatgga aagcctgcac cgcatgcccc atagcgaaac tctgtggatc
     8761 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
     8821 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
     8881 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
     8941 gttttttcaa tgttacccaa agcgcgattc tttgctaata tgttcgataa cgaacattta
     9001 tgagctttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
     9061 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
     9121 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
     9181 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
     9241 cgtgaagtgc tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca
     9301 catcgtccgc atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat
     9361 ctgggtaaac gcaccagcct gccgggatca attggtttgc gttttggcgc aaattcagtg
     9421 ttaaaaccgg aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt
     9481 ctggtactcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact
     9541 cgcgccacct ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
     9601 accggcaaaa aatatagctg gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg
     9661 aaacagttct tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
     9721 ggcagccata ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac
     9781 gaagataaac gcattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc
     9841 accgatgtgg aatacaaagg tgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat
     9901 tacctgctga aagtgtataa cgcgcacttt aaaaagcagc taagccgtga cgatatcgtc
     9961 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaagccatt
    10021 acccgtgatt acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta
    10081 ttcggcggta agctgaccac ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg
    10141 ccgtattatc agggtattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc
    10201 attgaaggcg accgcgacga ttacgccgct cgcctgcgcc gccgttaccc gttcctgact
    10261 gaatcgctgg cacgtcacta cgcgcgcact tacggcagca acagcgagct gctgctcggc
    10321 aatgcgggaa cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggaa
    10381 ctgaaatacc tggtggatca cgaatgggtc cgccgcaccg acgatgccct gtggcgtcgc
    10441 acaaaacaag gcatgtggct gaatgcggat caacaatctc gtgtgagtca gtggctggtg
    10501 gaatatacgc agcagaagtt gtcgctggcg tcgtaaatta acgtaaggtg atcaggtcag
    10561 atttcaatct ggcctgagac tgatgacaaa cacaaaactg cctgatgcgc tacgcttatc
    10621 aggcctacgt ggtttatgca atatattgaa tttgcatggt cttgtaggcc agataagacg
    10681 ttcacgtcgc atccggcatg aacaaagcgc actttgtcaa aaatctaacc tactttttaa
    10741 ttcagggaat taccgcaaag cccacgccat catacccaac gtaacaaaac tcaggcacgt
    10801 tccccactcg ccccgagaaa aatagcatta atgcgcccag cgtcagcata aaaatattga
    10861 gcagaaggta aattgttggc gtaaagagta aattgcaaac ctcaggaata gcggcaagcc
    10921 cagcccccag catggcaata accgctgcaa aaaattgcgt cagtattggg atgccgaaaa
    10981 aagtcattac cagcgaggtc aaaatccatt ttctattttg cattattctt tccattcttt
    11041 ctgaatggcg aaattatact ccccttgccc cttctggaca cttttccgaa atgatggcga
    11101 aaaaaacggg accctttgg
//
LOCUS       DADMFL010000125        11104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.126, whole genome shotgun sequence.
ACCESSION   DADMFL010000125 DADMFL010000000
VERSION     DADMFL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.126"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..608)
                     /gene="hdhA"
                     /locus_tag="JNZ27_003383"
                     /pseudo
     CDS             complement(1..608)
                     /gene="hdhA"
                     /locus_tag="JNZ27_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
     gene            complement(720..1748)
                     /locus_tag="JNZ27_003384"
     CDS             complement(720..1748)
                     /locus_tag="JNZ27_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY8665592.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1923..3437
                     /locus_tag="JNZ27_003385"
     CDS             1923..3437
                     /locus_tag="JNZ27_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY8665593.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIGLVIPLVWPIFAMGISGLGHMINSAGDFGPMLFGTGERLLLPFGLHHILV
                     ALIRFTDAGGTQEVCGQTVSGALTIFQAQLSCPTTHGFSESVTRFLSQGKMPAFLGGL
                     PGAALAMYHCARPENRHKIKGLLISGLIACVVGGTTEPLEFLFLFVAPVLYVIHALLT
                     GLGFTVMSVLGVTIGNTDGNIIDFVVFGILHGLSTKWYMVPVVAAIWFVVYYVIFRFA
                     ITRFNLKTPGRDSEVASSIEKAVAGAPGKSGYNVPAILEALGGADNIVSLDNCITRLR
                     LSVEDMSLVNVQALKDNRAIGVVQLNQHNLQVVIGPQVQSVKDEMAGLMHTVQA"
     gene            3447..4619
                     /gene="malY"
                     /locus_tag="JNZ27_003386"
     CDS             3447..4619
                     /gene="malY"
                     /locus_tag="JNZ27_003386"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY8665594.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGKEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4722..5723
                     /gene="add"
                     /locus_tag="JNZ27_003387"
     CDS             4722..5723
                     /gene="add"
                     /locus_tag="JNZ27_003387"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY8665595.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5759..6799)
                     /locus_tag="JNZ27_003388"
     CDS             complement(5759..6799)
                     /locus_tag="JNZ27_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8665596.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7042..7167
                     /gene="blr"
                     /locus_tag="JNZ27_003389"
     CDS             7042..7167
                     /gene="blr"
                     /locus_tag="JNZ27_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY8665597.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7440..7655
                     /gene="ydgT"
                     /locus_tag="JNZ27_003390"
     CDS             7440..7655
                     /gene="ydgT"
                     /locus_tag="JNZ27_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY8665598.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7741..8181
                     /locus_tag="JNZ27_003391"
     CDS             7741..8181
                     /locus_tag="JNZ27_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY8665599.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8258..8839
                     /gene="rsxA"
                     /locus_tag="JNZ27_003392"
     CDS             8258..8839
                     /gene="rsxA"
                     /locus_tag="JNZ27_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY8665600.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8839..9417
                     /gene="rsxB"
                     /locus_tag="JNZ27_003393"
     CDS             8839..9417
                     /gene="rsxB"
                     /locus_tag="JNZ27_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY8665601.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9410..>11104
                     /gene="rsxC"
                     /locus_tag="JNZ27_003394"
     CDS             9410..>11104
                     /gene="rsxC"
                     /locus_tag="JNZ27_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8665602.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2587 a         2859 c         2794 g         2864 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt tttcacccag gtcaaatgcc atatttctga ccagatgact ggccgcagct
      121 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
      181 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
      241 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ctgccattgg catatcaaac
      301 ggtttaggac caccgccacc ggcgttgtta accaggatat caactttacc cagtttactg
      361 acggcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acgccaggca
      421 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
      481 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
      541 atacctgcac ccgcacctgt gatgatggcg cattttccgt cgagtctcag gttgtcagaa
      601 ttaaacacat aaacctcctg tgaacttcag taaaaataaa aggcaccgcc tgtgtgccac
      661 attttttatc attaaccttg aggaaaggac actaccaagt atagcagata aagagaggat
      721 tatttcgccg caatgagccg ggcgggaata ataagattgc gtgaatgcgt ctcttcatgg
      781 gtgatttttt gcatcatgcg atccgcaagt gtgataccaa gttcccgcgc tggcgtgctg
      841 gcccaggtaa caggtatatc atcaagtgtg gttggtgtcg catcggtaaa tgccgccagc
      901 gaaacctgtt gctcaaagta acgatcgact ccgctttccc cgctttgccg ccctgctttc
      961 agcaaaccaa accatgcccc catcgcaata gtttcgttat agcaaaccac ggcactgatg
     1021 gtcgggttat gacgtaaaag cgccgtgata gcttccgcgg cttgcttctg gctggaagtg
     1081 cactccaaca cccaatcgct gtgaaacggc aggccaaatt ttagtagagt tgcacaatag
     1141 ccccccaccc gttctgcacg ggttaatgag gaactttgcc ctcccagcca ggcgatccgc
     1201 tgatgcccat tgcgaatgag atgctccgtc aacaactgtg cagcctgcat gttgtccggg
     1261 cgaaccgtat caacatcatc aagataactg gcacgggaag cgaaaatcac cgggatagct
     1321 ttttcttctg ccatccgccg caggtcatcg ctgcttcctg cagccccggc aattaccaca
     1381 ccatcgacac cctgattcag taacagtgaa aaccgctgtg ccagctgctc gccgtcttta
     1441 ccgccgtgaa gcaaaaaaac catccgtccc tgcgcttcca gagcttccgt caatccggcc
     1501 gtcaattcgg cgtaaaacgg cgcagataaa tcacggacga tcaaaccaat gacgccactt
     1561 tgcccgccgc gcagcgccga cgcctggcga ttgcgcacga atcccagctc ttcaatggcg
     1621 gcgttaacgc gttctcctgt ggcggtagag attcgccctt tgccactaag caccagcgaa
     1681 acggtactta ccgacacgcc cgcagccagc gcaacatcat gaatggttat ttttttggcg
     1741 gtagccatgc agatgaccta ctccctgatt atgtgacaga taaaacgttt taccttttat
     1801 ttatcttata cccgctatta tcgttgcgta atgtgattta tgactcacta aaatttgata
     1861 aaacgtttta tcttctcgcg caatttactg aatccagatt gttctctacg aggagtcgtt
     1921 ttatgacggc gaaaacagca ccgaaagtca cgctgtggga gttcttccag cagttaggca
     1981 aaaccttcat gttacccgtg gcattattgt cgttctgcgg cattatgctc ggcattggta
     2041 gttctcttag cagccatgat gtcataaccc tgatcccggt cctgggcaac cccgtgttgc
     2101 aggctatctt tacctggatg agtaagattg gctcgtttgc ttttagtttc ctgcctgtca
     2161 tgttctgtat cgccatcccg ctgggcctgg cacgcgaaaa taaaggcgta gcggcattcg
     2221 ctggcttcgt cggttatgcg gtaatgaacc tcgcggtaaa cttctggttg accaataaag
     2281 gcattctgcc aaccacagat gccgccgttc tgaaagccaa taacatccag agcattcttg
     2341 ggatccagtc gatcgatacc gggatcctcg gtgcggtgat cgccggtatt atcgtctgga
     2401 tgctgcatga gcgtttccat aatatcggcc tggtgattcc attagtctgg ccgattttcg
     2461 ccatgggtat tagcggcttg ggccatatga taaacagcgc gggtgatttc ggaccgatgc
     2521 tgtttggtac cggtgaacgt ctgctgttac cgtttggtct gcatcacatt ctggtggcat
     2581 taattcgctt taccgacgca ggcggcacgc aggaggtctg cggtcaaacc gtcagcggcg
     2641 cactgaccat cttccaggcg caattgagtt gcccgaccac tcacggtttt tctgaaagcg
     2701 tcacgcgttt cctttcgcaa ggtaaaatgc ctgcgtttct cggcggtctg ccaggtgcag
     2761 cgttagctat gtatcactgc gcgcgcccgg aaaatcgcca taaaattaaa ggtctgctga
     2821 tttctggcct gatcgcctgc gtcgttggcg gcactaccga accgctggaa ttcctgttcc
     2881 tgttcgtagc gccagttctg tatgtcatcc acgcgctgtt aaccggcctc ggcttcaccg
     2941 tcatgtctgt gctcggcgtc accatcggta ataccgacgg caatatcatc gacttcgtgg
     3001 tgttcggtat tttgcatggt ctgtcaacca agtggtacat ggtgccagtg gtggcggcaa
     3061 tctggtttgt cgtttactac gtcatcttcc gtttcgctat cacccgcttc aatctgaaaa
     3121 ccccggggcg cgatagcgaa gttgccagct caatcgaaaa agccgttgcc ggtgcgccgg
     3181 gtaaatcagg ttacaacgtt cctgcaatcc tcgaagcatt aggcggtgcc gacaatattg
     3241 tcagcctcga taactgcatt acccgtctgc gtttgtctgt ggaagatatg tcgcttgtta
     3301 atgtgcaggc actgaaggac aatcgggcaa ttggcgtagt acaacttaat caacataacc
     3361 tgcaggttgt tatcgggcca caagttcagt cagtaaaaga tgaaatggcc ggtctgatgc
     3421 atactgtcca ggcataagga taagatatgt tcgatttttc aaaggtcgtg gatcgtcatg
     3481 gcacatggtg tacacagtgg gattatgtcg ctgaccgttt cggcactgct gacctgttac
     3541 cgttcacgat ttcagacatg gattttgcca ctgccccctg cattatcgag gcgctgaatc
     3601 agcgcctgat gcacggcgta tttggctaca gccgctggaa aaacgatgag tttctcgcgg
     3661 ctattgccca ctggttttcc acccagcatt acaccgcaat tgatacgcag acggtggtgt
     3721 atggcccttc tgtcatctat atggtttcag aactgattcg tcagtggtct gaaacaggtg
     3781 aaggcgtggt gatccacacg cccgcctatg acgcatttta caaggccatt gaaggtaacc
     3841 agcgcacagt aatgcccgtt gctttagaga agcaggctga cggctggttt tgcgatatgg
     3901 gcaagctgga agccgttttg gcgaaaccag aatgtaaaat tatgctcctg tgtaacccac
     3961 agaatcctac cgggaaagtg tggacgtgcg atgagctgga gatcatggct gacctgtgcg
     4021 agcgtcatga tgtgcgggtt atttccgatg aaatccatat ggatatggtc tggggcgagc
     4081 agccgcatat tccctggagt aatgtggcgc ggggagactg ggcgttgctg acgtcgggat
     4141 cgaaaagttt caatattccc gcgctgaccg gtgcttacgg gattatagaa aatagcagca
     4201 gccgtgatgc ctatttatcg gcactgaaag gacgtgatgg gctttcttcc ccttcggtac
     4261 tggcgttaac tgcccatatc gccgcctatc agcaaggtgc accgtggctg gatgccttac
     4321 gcgtctatct gaaagataac ctgacgtata tctcagataa aatgaacgcc gcgtttcctg
     4381 aactcaactg gcagatcccg caatccactt atctggcctg gcttgatcta cgtccgttga
     4441 atattgacga caacgcgttg caaaaagcgc ttatcgagca agaaaaagtc gcgatcatgc
     4501 cggggtatac ctatggtaaa gaaggtcgtg gttttgtccg tctcaatgcc ggctgcccac
     4561 gttcgaaact ggaaaaaggt gtggctggat taataaacgc catccgcgct gttcgctaac
     4621 cccaattgcg caacgtaaaa atcgttgcgc aatcgtggat ttttaccctg ctttgttttt
     4681 ataatggtgc gcacttttat atccagaaaa agagtgcgac catgattgat accaccctgc
     4741 cattaactga tatccatcgc caccttgatg gcaacattcg tccccagacc attcttgaac
     4801 ttggccgcca gtataatatc tcgcttcctg cacaatccct tgaaacactg attccccacg
     4861 ttcaggtcat tgccaacgaa cccgatctgg tgagctttct gaccaaactt gactggggcg
     4921 ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc atttgaaaac attgaagatg
     4981 cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc accaggctac atggcaatgg
     5041 cacataagct gcctgtagcg ggtgttgtcg aagcggtgat cgatggcgta cgtgaaggtt
     5101 gccgcacctt tggtgtgcag gcgaagctta tcggcattat gagccgaacc ttcggcgaag
     5161 ccgcctgtca gcaagagctg gaggcctttt tagcccaccg tgaccagatt accgcacttg
     5221 atttagctgg tgatgaactt ggattcccgg gaagtctgtt cctttctcac ttcaaccgcg
     5281 cgcgtgatgc gggctggcat attaccgtcc atgcaggcga agctgccggg ccggaaagca
     5341 tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg acatggcgta aaagccattg
     5401 aagatcgggc gctgatggat tttctcgccg agcaacaaat tggtattgaa tcctgtctga
     5461 cctccaatat tcagaccagc accgtagcag agctggctgc acatccgctg aaaacgttcc
     5521 ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc cggcgtacag ggagtggata
     5581 tcattcacga atataccgtt gccgcgccag ctgctgggtt atcccgtgag caaatccgcc
     5641 aggcacagat taatggtctg gaaatggctt tcctcagcgc agaggaaaaa cgcgcactgc
     5701 gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca aattgcacca tccatttttc
     5761 acgcaaggca caacgtcgcg cgatgtttgg cggattcgat gcccagctca atcaactcca
     5821 ttacctggat tgcctggctt gccggaaccg gattttcacc atcgccattt aacgcatcac
     5881 gaatcgccgc atagtaagcc ggataattcc caggcaccgt caacagcgtt tcttcgacac
     5941 gttcctcacc ttccacgcgg gtaagtacgc catcacgcat atcgtagccc cagtcttcct
     6001 gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg atcgaggcca tatttcacat
     6061 aactgcctcg ggatccatgc acgatatacc gtgctgactc agcggctgcc agcatggtac
     6121 cgtgtaaaat gactcgccgc tgtggatagg acaagatggc gtggaaataa tcggtcgact
     6181 gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct gaccggtaaa ccaaatagcg
     6241 taatggcctg atcaagaaga tgcggtgcta aatcgtacca gataccgctg cctgggccgc
     6301 cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg gtcaaaatga gactcaaagt
     6361 aagcaacttc acccagcacg ccttccgcga gcaaaccttt tagcgtcaag aaatcgctat
     6421 cccaacgacg gttatggaat acagacagca cacgccccag gctttttgcc agcgcatcca
     6481 gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc aacgaccaca tgtttacccg
     6541 cctcaagcgc cgctttggct aacgggaaat gggtatcgtt gggtgtagga atgacaatca
     6601 ggtctatgtt gggatcgtta aacagatgct tcggctcaga gacaaccgta accgttggcc
     6661 agtcggcttt tacttttgtt tcatcactgc tggagattac cgccagttcc agcccgggcg
     6721 tgcccgcaat caggggcgca tggaaggttt tgctcgcata accataccca atcaacccaa
     6781 cacggatgtt gtcgctcatg tcattgcctc tcattgaagt atgatggcta tttgacacta
     6841 tcctttaccc acgctcaaca gtttaataac ctgccagcaa taagggatgt tgtttaactt
     6901 aagtcaaaaa aatagcgaat tttccaacga caaaagctaa atatcgcaaa aacctcagta
     6961 aaaatcttgc tggagctatt attgctaagt aacatttacc ccctgaagtt aatggatcaa
     7021 tcaagagaga tgtgggctgt aatgaatcgt cttattgaat taacaggttg gatcgttctt
     7081 gtcgtttcag tcattcttct tggcgtggcg agtcacattg acaactatca gccacctgaa
     7141 cagagtgctt cggtacaaca caagtaagct ctgcacttgt ggagcgacat gctgcccgtc
     7201 cgggtgcatg ttttcacttg tcggatatta aaccaggaat ttattatctt gttcgatgtt
     7261 gttggtgatt gtcagggata gtaaagcgcg ttactctttt cagagcagtt tttcatccct
     7321 gaatatttgc catcagcgca ttcttattat tgtaataaca caagcgttca cttttggtga
     7381 aattttatca tcactaataa tacctacccc tttggtatta attattttat ggacctttta
     7441 tgactgttca ggactactta ttaaaatttc gcaaaatcag ttcactcgaa agtctggaaa
     7501 aactctacga ccatcttaat tacaccctga cggacgatca ggaactgatc aatatgtatc
     7561 gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg gcgtttgttt gacctcggcc
     7621 aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa gtagcgatcg gttataaaag
     7681 ccttataata acgcccctaa aattagattt gcctgtttgc gtcgctattc tggagaacct
     7741 atgaccacca caacgccaca acgtattgga ggctggttgc ttggcccgtt ggcctggctt
     7801 ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt ataccgctgc gttatcttct
     7861 ccgcaaacat ttcaaacgct cggcggacaa gcactgacga cacaaatctt atggggcgtt
     7921 tcttttatta ccgctatcgc catgtggtat tacacactgt ggttgaccat cgctttcttt
     7981 aaacgtcggc gttgtgttcc taaacactat attatctggc tgcttatttc cgtattgctg
     8041 gcagtaaaag cattcgcttt ttcacccgtg gaagttggca tagccgttcg ccagttgctg
     8101 tttactttgt tggcaaccgc attgatcgtc ccctatttca aacgctcgtc gcgggtaaaa
     8161 gcgacgtttg tgaatccgta ataaccttac agttaacctg ttgtcgcctg ctctggatta
     8221 acggataata ggcggctttt ttatttcagg ccgaaaaatg actgactacc tgttactgtt
     8281 tgtcggaact gtactggtca ataactttgt actggtcaag tttctcggtc tctgtccgtt
     8341 tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg gggctggcaa caacgtttgt
     8401 gatgacgctg acgtctattt gcgcctggct tatcgatacg tggattttga tcccacttaa
     8461 tctgatttac ctacgcaccc tggcatttat tctggtgatt gctgtggtcg tgcagttcac
     8521 cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt ctgctgggga tttttttgcc
     8581 gcttatcacc accaactgtg cagtgctcgg cgtggcgttg ctgaatatca atctcgggca
     8641 caatttcttg cagtcggcgc tgtacggttt ttccgccgct gtcggtttct cgctggtgat
     8701 ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct gatgtcccgg caccttttcg
     8761 cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct ctggccttta tgggctttag
     8821 tggtttggtg aagttgtaat gaatgctatc tggattgccg ttgccgccgt gagcctgctg
     8881 ggcctggcgt ttggcgccat tctgggttat gcctcccgcc gttttgcggt ggaagacgat
     8941 ccggtcgttg agaaaattga cgaaatctta ccgcagagcc agtgtggtca gtgcggttat
     9001 cccggctgtc gcccctacgc ggaagccatc agctgtaacg gtgaaaaaat caaccgttgc
     9061 gccccaggtg gcgaagctgt gatgctaaaa attgccgagt tgcttaatgt cgagccgcag
     9121 ccgctggatg gcgaagcgca agagctaacg cctgcgcgga tggtggcggt tattgatgaa
     9181 aataactgta ttggctgcac taaatgtatt caggcttgtc cggtagacgc catcgttggc
     9241 gctacccgtg ccatgcatac ggtaatgagt gatctctgta cgggctgcaa tttatgtgtt
     9301 gatccgtgcc cgacgcactg catctcgttg caaccggtcg cagaaacacc tgactcctgg
     9361 aaatgggatc tgaacaccat tcccgtgcgt atcattcccg tggaacacca tgcttaagtt
     9421 attctctgca ttcagaaaaa ataaaatctg ggatttcaac ggcggcatcc atccaccgga
     9481 gatgaaaacc cagtccaacg gtacccccct gcgccaggta ccactggcgc agcgttttgt
     9541 tattccactg aaacagcata ttggcgctga aggtgagttg tgcgttagcg tcggcgataa
     9601 agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa atgctgcctg ttcacgcgcc
     9661 cacctcgggt accgttacgg ctattgcgcc ccactctacg gctcatcctt cagctttagc
     9721 tgaattaagc gtgattattg atgccgatgg tgaagactgc tggatcccgc gcgacggctg
     9781 ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag cgcatacatc agtttggtgt
     9841 tgccgggctg ggcggtgccg gattcccgac aggcgttaaa ttgcagggtg gtggagataa
     9901 gattgaaacg ttgattatca acgctgctga gtgcgagccg tacattaccg ccgatgaccg
     9961 tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt cgcattcttg cgcatattct
    10021 gcagccacgc gaaattctta tcggcattga agataacaaa ccgcaggcga tttccatgct
    10081 gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg gtgattccaa ccaaatatcc
    10141 ttctggcggt gctaaacaat taacctacat tctgaccggg aagcaggttc cacatggcgg
    10201 gcgttcatcc gatatcggcg tattaatgca aaacgtcggc actgcttatg cagtgaaacg
    10261 tgccgttatt gatggcgagc cgattaccga gcgtgttgta accctgactg gcgaagcaat
    10321 cgctcgcccg ggcaacgtct gggcacggct gggaacgcca gtgcgtcatt tattgaatga
    10381 tgccggattc tgcccctctg ccgatcaaat ggtgattatg ggtggcccgc taatgggctt
    10441 taccttgcca tggctggatg tcccggtcgt aaagattacc aactgtctgt tggctccctc
    10501 tgccaatgaa cttggcgaac cacaggaaga acaaagctgc atccggtgta gcgcctgtgc
    10561 tgacgcctgc cctgtggatc ttttgccgca acagttgtac tggttcagca aaggtcagca
    10621 acacgataaa gctaccacgc ataacattgc cgactgcatt gaatgtgggg cttgcgcgtg
    10681 ggtttgcccg agcaatattc ccttggtgca atatttccgt caggaaaaag ctgaaattgc
    10741 ggctattcgt caggaagaaa agcgcgccgc agaagccaaa gcgcgtttcg aagcgcgcca
    10801 ggctcgtctg gagcgcgaaa aagcggctcg ccttgaacga cataagagcg cagccgttca
    10861 acctgcagcc aaagataaag atgcgattgc tgccgctctg gcgcgggtga aagagaaaca
    10921 ggcccaggct acacagccta ttgtgattaa agcgggcgaa cgcccggata acagtgcaat
    10981 tattgcagca cgggaagccc gtaaagcgca agccagagcg aaacaggcag aactggagca
    11041 aactaacgac gcagcaaccg ttgctgaacc acgtaaaact gccgttgaag cagctatcgc
    11101 ccgc
//
LOCUS       DADMFL010000126        10904 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.127, whole genome shotgun sequence.
ACCESSION   DADMFL010000126 DADMFL010000000
VERSION     DADMFL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.127"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..882)
                     /locus_tag="JNZ27_003395"
     CDS             complement(<1..882)
                     /locus_tag="JNZ27_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665603.1"
                     /translation="MNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQIN
                     VKAKLPQERSEKVEDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYS
                     AKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVP
                     KNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEA
                     HSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQ
                     SDISVRNKYSPAFDKWNE"
     gene            complement(863..1201)
                     /locus_tag="JNZ27_003396"
     CDS             complement(863..1201)
                     /locus_tag="JNZ27_003396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665604.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(1791..2732)
                     /gene="fabY"
                     /locus_tag="JNZ27_003397"
                     /gene_synonym="yiiD"
     CDS             complement(1791..2732)
                     /gene="fabY"
                     /locus_tag="JNZ27_003397"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY8665605.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(2777..3214)
                     /gene="dtd"
                     /locus_tag="JNZ27_003398"
     CDS             complement(2777..3214)
                     /gene="dtd"
                     /locus_tag="JNZ27_003398"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY8665606.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3211..4083)
                     /locus_tag="JNZ27_003399"
     CDS             complement(3211..4083)
                     /locus_tag="JNZ27_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY8665607.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4077..4676)
                     /gene="yihX"
                     /locus_tag="JNZ27_003400"
     CDS             complement(4077..4676)
                     /gene="yihX"
                     /locus_tag="JNZ27_003400"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY8665608.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(4775..5560)
                     /locus_tag="JNZ27_003401"
     CDS             complement(4775..5560)
                     /locus_tag="JNZ27_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8665609.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5594..6490)
                     /gene="yihV"
                     /locus_tag="JNZ27_003402"
                     /pseudo
     CDS             complement(5594..6490)
                     /gene="yihV"
                     /locus_tag="JNZ27_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
     gene            6658..7554
                     /gene="yihU"
                     /locus_tag="JNZ27_003403"
     CDS             6658..7554
                     /gene="yihU"
                     /locus_tag="JNZ27_003403"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY8665610.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7578..9110
                     /locus_tag="JNZ27_003404"
     CDS             7578..9110
                     /locus_tag="JNZ27_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665611.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDPILLSYMGFF
                     SMGCIFIGVFLMPGAVRRFGKKKGYIGGLLIWVLGDLLNYFFGGGSVSFVAFSCLAFF
                     GSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGFFPGWMLTQI
                     GYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRIVEEIEARKR
                     TA"
     gene            9153..10556
                     /locus_tag="JNZ27_003405"
     CDS             9153..10556
                     /locus_tag="JNZ27_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8665612.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            10624..>10904
                     /locus_tag="JNZ27_003406"
     CDS             10624..>10904
                     /locus_tag="JNZ27_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665613.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         2702 a         2775 c         2587 g         2840 t
ORIGIN      
        1 ttcattccat ttatcaaaag ccgggctata tttgttgcgc acagaaatat ccgactggtt
       61 aaacaattca gccatgcgat tttttatcat ctcgatatcc ggcgaaccag aaagactttg
      121 cgcataatac ggtcctgatg atgttgcata cattaatgcc aagcccacac ctgtcgccat
      181 gatgggcgca gccacggttt tggcaagggc gacgctgcga tggctatgcg cttcacaggc
      241 taaaggaaca tattccactg actgcggtaa ttccttacat gcctccctca tcattgcggt
      301 atgctttaca aacaacggtt tttcactgcc caaaaaaact ttctgcttaa ttgcactaag
      361 aaaacgcaaa tcaaaaataa ttgtttttgg tttattattt tttggcacat ttcctattat
      421 cccgctaaga accttccttg ctgcatggag atttttttgc acagcacata acacaattga
      481 tgaagccttc tccactgcat tattgatttt aattttaaaa tccgcatgat atacgttgac
      541 gggcaatgca agtatatctg gacctacatc ttttgctgag tatttattgg tgatcgacgg
      601 acgtgaaccc acgagaaaac ttagctgcaa attatgctct atattatcag ctgtttttgt
      661 ggtgtggtta aacatagaga tgccaccttt cattctttca cacatatcct caactttttc
      721 acttcgttct tgtggcaatt tggctttaac attaatttga tgatttttac ccgtgcattc
      781 cttaactaca aggggaatgt tatctaaagt gccatacagg ctaataacgt caccaggatt
      841 tttatcatgt aagtaagttt gattaatact tgaagcattc acaaagaggg tcaatattat
      901 aactgtttat aacttcacct acatcaagtg tgtagtgctt actctggaga ctgccatcct
      961 tatttaattg tatatcaatc aatatatccc cggactttgt caccatatct ttgccataaa
     1021 ccatttgcat agccatttcg aggagatttt tatctaactg attgcgggcg ttgagtaagc
     1081 gattaccaaa taatttatat actgttcccg tcattgccag cgctgcgctt tctggtactt
     1141 tgcgtttcca cagtgaggtt tctctatttt taaacaggtc atattcatta ttactcgaca
     1201 aaccatgctt cagttcgtta acacaatcgc acagttgctc ctcaccttcc attttaaggc
     1261 aaagtgaaaa ttgatttaat ggcgtttact tcggtgattt attactcatt agttgttttt
     1321 ctaaattaac aacagaataa ctttcttgtt taatgagatt aatattggca accattatag
     1381 cctccttccc acggtttaag agcaaacata caacataatg attcataaca tacagagcag
     1441 taaatgaatg atactgacaa tgtatgccag cccattatct ttgcataatg tttgtgagct
     1501 atagatttta aaaatattta tgaaaaaagt atggcaccag agggggtttt gactatcaaa
     1561 aatcgctcaa acctgaacta agatgatgag ttaagcatta tctgcaataa ttgccactca
     1621 gctctacaaa taatatttaa atgctcctga acagtgatgt agcaagacac gtatggtgcc
     1681 aacgaagaat aaaaatttat aaagttctta ttcaatataa ccggaatagt cagggactga
     1741 tgtaaatgac cggaatgaat gagagaggca gcaccgcccc tctccataag ctactcttct
     1801 tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg gcagaacgat atacgtgcct
     1861 tcaaacactg cacccggcgt ctcgtcgcca aagatttcga cctgcatctg cacccgtgct
     1921 tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg caccaaggtc ggctaccgca
     1981 tggggtttac cgctaatcgg tttgctgtag cggatatgcg catccgccag aataatcgtt
     2041 ccgccgaggt ggcgttcacg cagcatcagc cagataagcc cccaaccggt gagcgtcgcc
     2101 agcgagaata aactcccggc aaacagcgta tggtgcggat tctgattgcc ggtttccggc
     2161 atggtggtga taaatttttg cccggtatat tgttgaatac gcacgcccat tttttcacta
     2221 agcgggatat gttcgtacca cgcctgttgc agctgcgcgc accagtcgcc gcgatgcaaa
     2281 atatcatcca gagtggcgac gggcttaatc atcaaaaaat ggcgaatcgg cgtggtggtt
     2341 ggcgtggtga tttctccctg attaacaaac cccagcttgg cgaaaaactc caccgcgtct
     2401 tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac gcgccaccga ctccagggtc
     2461 atcgccatca gcgttcctaa ccctttgtcc tgcacgtccg ggtgaacggc cataaagcga
     2521 atggacgctt cattgtcggc attaatatac aatcggccta ccgccaccag attaccctgc
     2581 tcgtcgacga ccatctggtg atgcgccatc gcatcccacg cgtcgcgttc cgaacctttt
     2641 ggttgatgca ggggcttacg caacatttcc cagcgaaact gatagtaacg ctctaactct
     2701 tcttctgttt gtggaacctg aaggtgatac atagcgatac tctctcttgt ttcccgtgac
     2761 aaccctggaa gctggctcat acctgcaacc agaatgtcac ggggccatca ttgaccagcg
     2821 atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt catctcttgc tggcggcagc
     2881 gttcgacgaa atagtcatat aacgcctctg cgcgatccgg tgatgcgcct ttggagaaac
     2941 ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa ctgggaaacc accagtacac
     3001 tgccgcccgc ctgttgcacg ttgagattca tcttgccttc ggcatcgcta aagatgcggt
     3061 agccgagcac acgctcgcac agacggtttg ctttctgttc gtcgtcatcc ttttcgacac
     3121 ccaataacac caaaagtccc gcgccaattt cgcccgtcac ttctccctcc acggtgacgc
     3181 tggcacgggt tacgcgttga attaatgcaa tcatggttcg tcgtcttctt cttgttcagc
     3241 tgcttgtttt agtttgcggt attccccgag agtgacagta atttccgcgc caagcaagac
     3301 gatacaccat gtccagtaga cccaaacaaa gagaatgggg atcaccgcca gcacaccgta
     3361 aatgagctga tatgacggga acatggtgat ataaagcgcg aaacctttct ttcctgcttc
     3421 gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg cggttaggta cgcggatggt
     3481 aggaacaatg ctgtacagca accagaagga gatccacgac aacagcaacg gaaaaatacg
     3541 cagcacgttg tcgatgacag tattgagatc gctcgcccag cgcagggaga gcaaatagga
     3601 actgatcgcc agactggccc ctgccagcag cggccccagc gttaaaatca tccagtacac
     3661 ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc cagatggtat tcaacgcgct
     3721 atcgatggag tacatcaaca ataacgcggt gacgatcagc ccgcacgccc caacggcggt
     3781 catcttgttg gaattagcaa caaattgttc gatataccgc tgaataacat cgccagtagc
     3841 aggcagaaag ttggcaaaaa taaagtgacg taactgaatg ctgacgtcgg aaaacatggg
     3901 aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc actaatgaga gcaacgacac
     3961 ataggcaagg ttacctgcca gggttgtcat gttgtcctca tcaatacgtt gccagagtag
     4021 ttttagccag gcccatagtg gacgggtacg gtgcctggct ttgtcctgaa tggtttttag
     4081 cataacacct tcgcgaaata gtccgggatg gtggttttat ctttcaccag aatactggta
     4141 atgcctagct gattagctcc ttctatatta tcggcgttat cgtcgaaaaa gaccgtatcg
     4201 ctgggtgaaa aaccttccgc ctgcaaaaca tgctggtaaa ttcgtgcttc aggtttgcgc
     4261 atccccagat cttgcgacag atagatatgg tcagcagcat cacgaatttc cgggtattct
     4321 tccggccaga aggtggtatg caggcggttg gtattggaaa gcaccaccac gcgatgcccc
     4381 tgctcacgca gtttatgcat gatggcgatc acttccgggc gcagcgcaac aaacaccgcc
     4441 tgccagccgt gagagaactg ctcgtagctt agcggtagag ccatctcatg acacagcgcc
     4501 tctgcgaacg cttcgtcgct aatttcccca cgctcatgct gatgaaacgc ctcccccata
     4561 tgaaaactct tcttaagcga tgccagcgga atacgcgtta aatcgctcca ggctcccagc
     4621 acacggttaa agtcgatatc gacaatcaca ttacctaaat caaagatata gagcattttt
     4681 gcctcctttc gcgccacaag aaattaactg tagcgggaaa ggatgggttt ggctatgtgg
     4741 caaggtatga agccaggtta acggtcacaa aaatctacgc gtcttcctgg ggaagaatga
     4801 tttcaatttg gctgtcttgt aagcgtgatt ttagcgcagc gggcggcagc tcgtcggtaa
     4861 agagcgcagt gacctgtgcc acgttaccaa tttcaaccgc cgccgaagca tgatacttag
     4921 tgtgatcggc gaccagcaga atatttctcg cgtgcgccat catcgttttc accacgttag
     4981 cttcgtttac atcaaactcc atcaacgcgc catcgctctc aatcgcccca acgcttgtta
     5041 ccagataatc agcacgaaaa tcagccacaa aggacgccgc tgaagggcca atgatcccgc
     5101 tattatgaga gcgcaacgta ccgccgggca ccatcacttc aaagcgcggg ttgtggtaaa
     5161 gaatatgcgc cacacgcagg ctgttggtga ttatccgcaa atgattatgg ttaagtaacg
     5221 cccgggcaac atgctcaaca gtcgtaccaa tggtgataaa tattgttgaa ccatcaggaa
     5281 tatagtctgc caccgcttcg gcaatcgctt tttttttctc ggtttgcgaa acctcacgct
     5341 gctcgaacgc cgtattaacg acgctggaag cccgacccgc gccaccgtga tggcgcgtaa
     5401 tcaggccttg ctcgcttaat ttacgaatat cccggcggac cgtctgcgtg gagacatcca
     5461 gcagatttgc cagctcatca atattcatat acccacgctc ggcgatcagc atgaggagtt
     5521 ggtcgtgccg cgggttaccg gttagttcgg taaggctcat gaaaattcct cgaaaaacca
     5581 tcaccctggc attttataca aaaagtgaca aaaaagatcg ggtttgatca cagtcaggga
     5641 tcccggctcg tccaccggga cgtgtgcatt ttaacgccgc tacaccgctg gcgaagcgga
     5701 ctgactccgc taaatcccca cttgttgcca gcgccaccgc caaagcgccg tgaaaaacat
     5761 cacccgcacc tgtggtatct accacatcaa ctttgaaggc cggttgatgc tgacgcccac
     5821 cattttccag ccagtcgcag cctgcgctac cctaggtcac atagacatga ccatttgtga
     5881 gcgtttgtgc ctgttttagc gcactggcca tctctttcac gcccgttaag cgcgccagac
     5941 ccggttctga aaaggccgcg tgatcgctta atgccaccag ctcactgata tcctgcggcg
     6001 taatgtcccc gtccagaacg gtcatcacac ccgcctgacg ggccagggtg aaggcttttt
     6061 tagcgccgtc gtgccagcgt acatctgcca gcacaacatc ccactgagag aaatcaattt
     6121 cctccaacca ctctgcgtca ggcagcagat ccgggctggg gtagttaatg attatccgct
     6181 cgcctttggt atccaccatg atggcggatt gcgaagattt cgcctggtta taccgtttgg
     6241 tgtaacgggt gttaacgccc caggattcca gctctgctag caggctgttg cccgcgtcgt
     6301 catcacctac gcgaccaata aaatcgacct gcgcccccag ccttgccgcc gcaaccgctg
     6361 ccgtcgccgc tggcccgcca ccaacttccg tataatttct cgccacgtat ttaccgctct
     6421 ccgtcggtaa cccttccacg taatagatgc gatccatcac ggttatacct acacaagcaa
     6481 cacgaatcat ggtcattcct taaacatttt gggatgcgtt cattttcatt tcaccaattg
     6541 tttaaaaagt gacggctgtc aattttttga cgataaatta caaatgcgat caaaaacaga
     6601 caagcaaagc atgtcataaa tgacaaaaag tgacatcact gtattcagga gagggttatg
     6661 gcagcaatcg cgtttatcgg tttaggacaa atgggttcgc caatggcgag caatttattg
     6721 cagcaagggc atcaacttcg cgtctttgat gtgaacgccg aagctgtgcg gcatctggta
     6781 gacaaaggtg cgactcccgc cgccaacccg gtgcaggccg ctaaagatgc cgaatttatc
     6841 attaccatgt tgccgaatgg cgatctggtg cgcaacgtgt tgttcggtga aaacggcgtt
     6901 tgcgaaggct tatctaccga tgcgctggtc attgatatgt ccaccatcca tccgctgcaa
     6961 accgataaat tgattgccga tatgcaagcc aaaggcttca gcatgatgga tgttccggta
     7021 ggccgtactt ctgcaaatgc cattaccggt actctgttac tgctggctgg cggcaccgct
     7081 gaacaagttg aacgtgccac accgatcctg atggcgatgg gcagtgagtt gatcaacgct
     7141 ggcggtccgg gcatggggat ccgcgttaag ctcatcaaca actacatgag catcgcgctc
     7201 aatgcgcttt cggcagaagc cgccgttttg tgcgaagccc tgaatcttcc cttcgatgtt
     7261 gccgtcaaag tgatgagcgg taccgccgcc ggtaaaggtc acttcaccac ttcctggcca
     7321 aacaaagtcc tcagcggaga tctttctccc gccttcatga tcgatcttgc ccataaggat
     7381 cttggcatcg cccttgatgt cgccaaccag ctgcatgtgc caatgccgct gggggccgcc
     7441 tcacgggagg tttatagcca ggcgcgcgca gcgggtcgcg gtcgccagga ctggtccgcc
     7501 attctggaac aggtccgtgt cagtgccggg atgactgcca aagtaaaaat gtaacgactg
     7561 gataaaggaa taaatgaatg aataagtaca ccatcaacga cattacgcgc gcatcgggcg
     7621 gttttgccat gctggcggtc gatcagcgcg aagccatgcg catgatgttt gccgcggctg
     7681 gggcacccgc tccggtagcc gatagcgttt taactgattt caaagttaac gctgcaaagg
     7741 ccctctcgcc ttatgcctcg gcgattctgg tagatcaaca attctgctac cgccaggtgg
     7801 ttgagcaaaa cgcgattgcc aaaagttgcg ccatgattgt cgccgccgat gagttcattc
     7861 ctggcaacgg tattccggtc gatagcgtgg ttattgaccg caaaatcaat ccgctacaga
     7921 tcaaacaaga cggtggcaaa gccttaaaac tgctggtgct gtggcgtagt gatgaagatg
     7981 cgcagcaacg tctggatatg gtgaaagagt tcaacggact gtgccactca cacggtctgg
     8041 taagcatcat tgagccagtc gtccgcccac cgcgtcgtgg cgataaattc gatcgcgaac
     8101 aagcggtcat cgatgccgcc aaagagctgg gcgacagtgg cgctgacctc tacaaagttg
     8161 aaatgccgct ttatggcaaa ggtccgcaac aagagcttct ctgtgcttca caacgtctga
     8221 atgaccatat caatatgcca tgggtgatcc tctcttccag tgtcgacgaa aaactgttcc
     8281 cgcgtgccgt acgcgtggca atgacggcgg gcgcatcggg attcctggca ggccgtgcag
     8341 tctgggcatc ggtcgtcggt ttaccagaca acgagctgat gctccgtgac gtttgcgcac
     8401 cgaaattaca acaacttggc gatatcgtcg acgaaatgat ggctaaacgc ccactgttca
     8461 ttctgtgtat tgccaacctc tgcaccttag gggcgtttaa cgtcaagctc gccattcagg
     8521 tctattacac ccagtacgta cttaacgatc ccatcctgtt gtcgtatatg ggatttttca
     8581 gcatgggctg tattttcatc ggcgtgttcc tgatgcctgg cgcagtcaga cgttttggta
     8641 agaagaaggg ctatatcggc ggcctgctga tttgggtgct gggcgatctg ctcaactatt
     8701 tcttcggcgg cggatcggtc agctttgtgg cgttctcctg cctggcgttc ttcggctcag
     8761 cgtttgttaa cagcctgaac tgggcgctgg tttccgacac cgtcgagtac ggcgagtggc
     8821 gcaccggcgt gcgttcggaa ggcacggtct acaccggctt caccttcttt cgcaaagtgt
     8881 ctcaggcgct ggctggtttc ttccccggct ggatgctgac gcaaattggc tatgtgccga
     8941 acgtcgcaca ggctgaccac accattgaag ggttacgcca gttgatcttc atctacccaa
     9001 gcgcactggc ggtagtcacc attgtggcga tgggttgctt ctacagcctg aacgagaaga
     9061 tgtatgtccg cattgtggaa gagatagaag cccgtaaacg cacggcgtaa taataataaa
     9121 cgacgccctg cggggcgtta taaggagtga ttatgtctga ccataatcca ctgacattaa
     9181 aactgaatct gcgggaaaaa atcgcctatg gtatgggcga cgtcggttcg aatttaatgc
     9241 tctgcatcgg tactctgtat ctcctcaaac tttataccga tgaattaggg atgcctgctt
     9301 actatggcgg gattatcttt ctggtcgcga agttttttac cgcgtttacc gatatgctca
     9361 ccggattttt actcgactcg cggaaaaata ttgggcctaa aggcaaattt cgtcccttta
     9421 ttttatatgc agcagttccg gcggcgttaa ttgcaacgct ccagtttatc gccaccacct
     9481 tttgtttgcc ggttaaaacg acgattgcca ccgcgctgtt tatgatgttt ggactttcat
     9541 acagtctgat gaactgctcg tatggtgcga tgatcccggc aattaccaaa aacccgaatg
     9601 agcgcgcgca gctcgcggct taccgccagg gtggtgcaac catagggcta ttgatttgta
     9661 ccgtggcgtt tattccgttg cagtcgcttt tttctgactc aaccgtcggt tatgcctgtg
     9721 cggcacttat gttctccatt ggcggcttta tttttatgat gctgtgctac agaggcgtca
     9781 aagagcatta tgtggacaca gcgccaaccg gacataaagc cagtattctc aaatcttttt
     9841 gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat tgctaacctg tgtactctgg
     9901 cggcatttaa tatcaaactg gcgattcagg tctattacac ccagtatgtg ctgaatgata
     9961 ttaatttatt gtcgtggatg ggattcttca gtatgggatg catcctcgtc ggcgtattac
    10021 tggtgccagt aactgtaaaa tgttttggta aaaaacaggt ttatttagct ggcatggtgc
    10081 tatgggcggt gggtgatata ctgaattatt tctggggaag taactctttc actttcgtca
    10141 tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa cagcctgaac tgggcactgg
    10201 taccagatac cgtagattac ggtgaatgga aaaccggtat tcgtgccgaa ggttctgttt
    10261 ataccggtta taccttcttt cgtaaaattt ctgccgcact tgctggcttc ttgccaggca
    10321 ttatgctgac gcaaattggt tatgttccca acatcgcgca aagcgatgcg acattacagg
    10381 gtttgcgtca gctcattttt atctggcctt gtgcactggc aattattgct gcattgacga
    10441 tgggattctt ttacacactc aatgaaaaac ggtttgcatt gattattgag gaaatcaacc
    10501 aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc ttccgtaacg ttataaatgt
    10561 caatagccgc tatttccatc ctggtgggtg gcggcctccc tacgtttaaa aaatggactt
    10621 attatgaaaa agattaatgc aataatttta ttatcttctc tgacctctgc ctcggtattt
    10681 gctggcgcat acgtcgaaaa cagggaggca tataatcttg cctccgatca gggcgaggtt
    10741 atgctacgtg tgggttataa cttcgatatg ggcgcgggta ttatgttaac caatacctac
    10801 acctttcagc gagaagatga actaaaacat ggctataatg aaattgaagg ttggtatcct
    10861 ttattcaaac caaccgataa attaaccatc cagcccggtg gctt
//
LOCUS       DADMFL010000127        10815 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.128, whole genome shotgun sequence.
ACCESSION   DADMFL010000127 DADMFL010000000
VERSION     DADMFL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.128"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            87..1178
                     /locus_tag="JNZ27_003407"
     CDS             87..1178
                     /locus_tag="JNZ27_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8665614.1"
                     /translation="MDLLIVLTYVALALATAALGGVFLVSGLILLMNYNHPYTFTAQK
                     AVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNI
                     KTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQD
                     ALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQV
                     LIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFH
                     GKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAV
                     YSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="JNZ27_003408"
     CDS             1249..1857
                     /locus_tag="JNZ27_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY8665615.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="JNZ27_003409"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="JNZ27_003409"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY8665616.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIHGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="JNZ27_003410"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="JNZ27_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY8665617.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5377..6483
                     /gene="yiaY"
                     /locus_tag="JNZ27_003411"
     CDS             5377..6483
                     /gene="yiaY"
                     /locus_tag="JNZ27_003411"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY8665618.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDSSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPI"
     gene            6693..8231
                     /locus_tag="JNZ27_003412"
     CDS             6693..8231
                     /locus_tag="JNZ27_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8665619.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8450..8605)
                     /locus_tag="JNZ27_003413"
     CDS             complement(8450..8605)
                     /locus_tag="JNZ27_003413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665620.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8776..9099
                     /locus_tag="JNZ27_003414"
     CDS             8776..9099
                     /locus_tag="JNZ27_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8665621.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9105..10241
                     /locus_tag="JNZ27_003415"
     CDS             9105..10241
                     /locus_tag="JNZ27_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8665622.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKPTRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10238..>10815)
                     /locus_tag="JNZ27_003416"
     CDS             complement(10238..>10815)
                     /locus_tag="JNZ27_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8665623.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2658 c         2977 g         2603 t
ORIGIN      
        1 gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac
       61 tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt gcgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcat ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcgcacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaataaagca cattgtttaa tgaatacaat gtgcttttta
     5281 ttagattaat tttgataatt aataataatg atcatgtttt cgaatgaaaa tcctcagtaa
     5341 gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt
     5401 ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat
     5461 tatggattta cccgtacctt aattgtcact gacaatatgt taacgaaatt aggtatggcg
     5521 ggtgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc
     5581 caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agaaaataat
     5641 tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg
     5701 ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa
     5761 ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt
     5821 ttctgcatca tcactgacga agcgcgtcat aacaaaatgg cgattgttga taagcacgtg
     5881 actccgctgc tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc
     5941 gccgcaacgg gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc
     6001 acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga aaacctgccg
     6061 ttagccgttg aagatagcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc
     6121 cttgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag
     6181 ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttctgct gccgcatgtt
     6241 caggtattca acagcaaagt cgccgctgca cgtctgcgtg actgtgccgc tgcaatgggc
     6301 gtgaacgtga caggtaaaaa cgacgcggaa ggtgctgaag cctgcattaa cgccatccgt
     6361 gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa
     6421 gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggctttac taatccgatc
     6481 taggcaactc acgaagaaat tgtggtgatt tatcgcgccg caatgtaatc atcattccaa
     6541 caacggctgg caaattgtca gccgcttttt caactatctc tgtaatcctt gcccgtaaat
     6601 tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc
     6661 cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga
     6721 ttaagcccgg cgagtatggt ttccccatca agttaaaagc ccgctacgac aactttattg
     6781 gcggtgaatg ggtagcccct gccgatggtg agtattacca gaacctgacg ccggtgaccg
     6841 ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg
     6901 ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc
     6961 tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct
     7021 gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc
     7081 atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata
     7141 gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt
     7201 ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact
     7261 gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaattg
     7321 tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt ggggaaattg
     7381 gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag
     7441 tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg ctggagttgg
     7501 gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg
     7561 ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt tgcacctgtc
     7621 cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc
     7681 gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg ggcgcgcagg
     7741 tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg
     7801 ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact
     7861 acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt
     7921 ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga
     7981 acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat ctggcctata
     8041 agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac gcttacccgg
     8101 cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga
     8161 tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg
     8221 ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac
     8281 gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc
     8341 cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc
     8401 cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg
     8461 tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg aggccaatca
     8521 gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac
     8581 tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa acctcgtcct
     8641 gttgaaaaac cagacagcac atttcagaaa gtatataaaa aacaatatgt cgttatataa
     8701 ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata
     8761 ttctttgagg gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt
     8821 ctggtgatat tctatgggat tattatttta catgatattc cctacctgat ttctaaaaaa
     8881 cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgctg
     8941 catgttatct ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg
     9001 ggaatgcaaa tccatgactc ctccgttgtg caactacaac agcgtattgc cgggctggag
     9061 aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta
     9121 ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata
     9181 aatggaccat ttccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt
     9241 taatgaacta taaccatccg tataccttca aggcgcaaaa agcggttatt tctattcctg
     9301 ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta
     9361 aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggtgcgg gtggatcggc
     9421 tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg
     9481 agatggcggc gaatactcag caggcaaagc ccacgcggga taaattcgct aaagagtatc
     9541 agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg
     9601 tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa
     9661 aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc
     9721 tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga
     9781 gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc
     9841 tacgtccggt gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc
     9901 agaactcctt gctgcgactg gcccctggcg acgatgcgga agtggtgttt aatgctctgc
     9961 caggtaaggt attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt
    10021 atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg
    10081 cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt atttacgccc
    10141 aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctattac
    10201 gcatgaccag ctgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt
    10261 gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat
    10321 ccagcacata ctggacccgc cagggcaggt attcccgttt acggaagata gccaccagat
    10381 ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcagatcgc
    10441 tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg
    10501 ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac
    10561 tcccggcgac gttgaagcgc cagatattcg cgtcgtcgat catcatcgag tcgatcaaaa
    10621 tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct
    10681 ggctggcata agcggttacg gcatattgtg taataaaact ggcaaccagc gattcatctt
    10741 ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa
    10801 aggcattacg ctcaa
//
LOCUS       DADMFL010000128        10698 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.129, whole genome shotgun sequence.
ACCESSION   DADMFL010000128 DADMFL010000000
VERSION     DADMFL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.129"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            126..1823
                     /locus_tag="JNZ27_003417"
     CDS             126..1823
                     /locus_tag="JNZ27_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY8665624.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     ITVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JNZ27_003418"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JNZ27_003418"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY8665625.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JNZ27_003419"
     CDS             3113..3397
                     /locus_tag="JNZ27_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8665626.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNZ27_003420"
     CDS             complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="JNZ27_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY8665627.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4093..4977)
                     /gene="fdxH"
                     /locus_tag="JNZ27_003421"
     CDS             complement(4093..4977)
                     /gene="fdxH"
                     /locus_tag="JNZ27_003421"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8665628.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNZ27_003422"
     CDS             complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="JNZ27_003422"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7450..7452),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8665629.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIRYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8269..9150
                     /gene="yddG"
                     /locus_tag="JNZ27_003423"
     CDS             8269..9150
                     /gene="yddG"
                     /locus_tag="JNZ27_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY8665630.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9409..10509
                     /gene="ompC"
                     /locus_tag="JNZ27_003424"
     CDS             9409..10509
                     /gene="ompC"
                     /locus_tag="JNZ27_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8665631.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2623 a         2743 c         2653 g         2679 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagattt
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcgataacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3481 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3541 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3601 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3661 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3721 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3781 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     3841 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     3901 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     3961 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4021 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4081 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4141 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4201 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4261 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4321 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4381 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4441 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4501 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4561 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4621 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4681 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4741 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4801 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     4861 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     4921 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     4981 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5041 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5101 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5161 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5221 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5281 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5341 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5401 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5461 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5521 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5581 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5641 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5701 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5761 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     5821 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     5881 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     5941 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6001 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6061 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6121 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6181 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6241 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6301 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6361 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6421 cctgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6481 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6541 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6601 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6661 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6721 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6781 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     6841 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     6901 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     6961 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7021 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7081 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7141 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7201 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7261 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7321 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7381 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7441 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7501 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7561 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7621 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7681 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7741 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7801 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     7861 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     7921 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     7981 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8041 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8101 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8161 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8221 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8281 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8341 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8401 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8461 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8521 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8581 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8641 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8701 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8761 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     8821 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     8881 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     8941 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9001 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9061 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9121 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9181 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9241 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9301 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9361 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9421 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9481 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9541 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9601 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9661 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9721 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9781 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
     9841 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
     9901 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
     9961 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10021 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10081 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10141 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10201 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10261 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10321 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10381 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10441 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat
    10501 cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg
    10561 ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat tcacctcaat
    10621 taatattatt ccatatcctc cggggacatc ttactataat atttattgat gagtgattat
    10681 gaccaatatt aatacagc
//
LOCUS       DADMFL010000129        10293 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.130, whole genome shotgun sequence.
ACCESSION   DADMFL010000129 DADMFL010000000
VERSION     DADMFL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.130"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..401
                     /locus_tag="JNZ27_003425"
                     /pseudo
     CDS             1..401
                     /locus_tag="JNZ27_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JNZ27_003426"
     CDS             484..741
                     /locus_tag="JNZ27_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665632.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNZ27_003427"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JNZ27_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY8665633.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNZ27_003428"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JNZ27_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAY8665634.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNZ27_003429"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JNZ27_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY8665635.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNZ27_003430"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JNZ27_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY8665636.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNZ27_003431"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JNZ27_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY8665637.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNZ27_003432"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JNZ27_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY8665638.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /locus_tag="JNZ27_003433"
     CDS             complement(8323..8844)
                     /locus_tag="JNZ27_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY8665639.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9120..9324
                     /locus_tag="JNZ27_003434"
                     /pseudo
     CDS             9120..9324
                     /locus_tag="JNZ27_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9318..9422
                     /locus_tag="JNZ27_003435"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JNZ27_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JNZ27_003436"
     CDS             9762..10019
                     /locus_tag="JNZ27_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY8665640.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2399 a         2735 c         2823 g         2336 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaggcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaacgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agc
//
LOCUS       DADMFL010000130        10256 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.131, whole genome shotgun sequence.
ACCESSION   DADMFL010000130 DADMFL010000000
VERSION     DADMFL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.131"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="JNZ27_003437"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="JNZ27_003437"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY8665641.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="JNZ27_003438"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="JNZ27_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY8665642.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASACTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMRLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="JNZ27_003439"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="JNZ27_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY8665643.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3268..4124
                     /locus_tag="JNZ27_003440"
                     /pseudo
     CDS             3268..4124
                     /locus_tag="JNZ27_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="JNZ27_003441"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="JNZ27_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="JNZ27_003442"
     CDS             5927..6439
                     /locus_tag="JNZ27_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY8665644.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="JNZ27_003443"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="JNZ27_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY8665645.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="JNZ27_003444"
     CDS             9032..9235
                     /locus_tag="JNZ27_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY8665646.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="JNZ27_003445"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="JNZ27_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY8665647.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2443 a         2671 c         2655 g         2487 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctgaca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccgcctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgaggc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga ccccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcaggc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagagccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtgga
//
LOCUS       DADMFL010000131         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.132, whole genome shotgun sequence.
ACCESSION   DADMFL010000131 DADMFL010000000
VERSION     DADMFL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.132"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNZ27_003446"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNZ27_003446"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY8665648.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNZ27_003447"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNZ27_003447"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY8665649.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JNZ27_003448"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JNZ27_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JNZ27_003449"
     CDS             3456..5216
                     /locus_tag="JNZ27_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HAY8665650.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTAYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JNZ27_003450"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JNZ27_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY8665651.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHN
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JNZ27_003451"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JNZ27_003451"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8665652.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JNZ27_003452"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JNZ27_003452"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY8665653.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2553 a         2496 c         2377 g         2529 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgcttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccataa ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 agttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatggggcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADMFL010000132         9912 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.133, whole genome shotgun sequence.
ACCESSION   DADMFL010000132 DADMFL010000000
VERSION     DADMFL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.133"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(75..413)
                     /locus_tag="JNZ27_003453"
                     /pseudo
     CDS             complement(75..413)
                     /locus_tag="JNZ27_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(441..1003)
                     /locus_tag="JNZ27_003454"
                     /pseudo
     CDS             complement(441..1003)
                     /locus_tag="JNZ27_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1207..1413)
                     /locus_tag="JNZ27_003455"
     CDS             complement(1207..1413)
                     /locus_tag="JNZ27_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY8665654.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1520..2083)
                     /locus_tag="JNZ27_003456"
     CDS             complement(1520..2083)
                     /locus_tag="JNZ27_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY8665655.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3458..5293)
                     /locus_tag="JNZ27_003457"
     CDS             complement(3458..5293)
                     /locus_tag="JNZ27_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665656.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5394..5681
                     /locus_tag="JNZ27_003458"
     CDS             5394..5681
                     /locus_tag="JNZ27_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8665657.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5653..7182
                     /locus_tag="JNZ27_003459"
     CDS             5653..7182
                     /locus_tag="JNZ27_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY8665658.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7352..8569)
                     /locus_tag="JNZ27_003460"
     CDS             complement(7352..8569)
                     /locus_tag="JNZ27_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8665659.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(9341..9912)
                     /locus_tag="JNZ27_003461"
                     /pseudo
     CDS             complement(9341..9912)
                     /locus_tag="JNZ27_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3121 a         2255 c         1849 g         2687 t
ORIGIN      
        1 cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa
       61 cccggggcgg ttcacttaca ccagacatcg ttattaagta gcagcaccat aagataagga
      121 gtggcatcgt tagccacagc ttccctactc cagaggtaat ataaaggggt gggctcgact
      181 gatttatctt tacgtcgctt acactgcaaa tgttcagaaa ttagtctatg cagctcacca
      241 gtaaaatcag caatcagaga aggaattgcc ttattcatac cagggcaagg tattaattta
      301 aactgtaata atttaatttt aggatgtgta gcagcagccc gatacagagt tgcaaggaca
      361 cacttttgcc agagggcgtt acaggaaagc ttaatgtttg attctgtata cataataaat
      421 caccttatag ttacaatagg tcaaaaaccg ctgtagccaa agttacgctg gcctgatgct
      481 ttagtgtcga gcttcgtcaa ataatccaga cgtaccaata agcgctggta ctgctctggg
      541 aaattaggat cgttaatatc caggatgtaa cgtccattag cagggaaatg aactaatccc
      601 tggtgatctt ccagttccaa ccctaaggca ctgtaccagg ctccggtaat taaccctctc
      661 agggtgtcag agcgctcata gtttcccaga tggtaatagg cgtctttgtt aaaaaaaggc
      721 agacgtgata atgacattta aaggactgcg tatattcttt cgcccagata atacaaaccg
      781 acgttgcgta ggtgcgtttg ccttgttgtt ttttacggtg ttgatctgca ttaattttcg
      841 cattcagcga gttacggaaa cgagatatct ctccggggat caggttaggg aaacaggtaa
      901 tgatatcccc gttgtcaatc acaggagggt aatggagatc cccccgtatt gctgttattc
      961 tggaagtgtt tttaatattc gtattgatag ttctcagaga tatatcacac tctagatatt
     1021 ttaattaagt atcagttcga tatcggcata ttgtacacta cctcaggtag atgatgagat
     1081 cagatacagg gatcaactga cggtgagcgc agtccccaca ttttatccag ggcttactgc
     1141 atattcccaa cagccattcg ttagccaggt agacgaatac cctgatttgc cgctggtttt
     1201 gctttcctat cgaagggccc atacatcatc acgcccacga aacaaccgac gaaataacgc
     1261 aactttctca tccgtggata atacgaaaac gcactgcaca gggctctgcg gtttacgtcg
     1321 ccattcaatc ccatgagctt caagtaatga gaccagccgg gtattttctg cccgcagtac
     1381 tgctagttca tcgctgtcag atttataaac catatgcgtt atcacttgta cctgtctccc
     1441 ggcggcacca cgactgaaat tcgtgtgaag gcaaatccac gcgataactt acgatagttc
     1501 ggacataagc ccccataatt cagttatgtc gtggtcgacc ccggttactt ttttcgggaa
     1561 gcctgctacg gcccagaata ataccgtgtt ctttctgatc ctgtttagcc agatagctaa
     1621 gggcgtaacg taagtcgtct accgcagatt tatcactata gtgaatcacg tgatcgatac
     1681 gcaccgggta tttgtcttta gcccggcaca gatgaaaata accctcccct tccgtgatcc
     1741 gcctccagat atctcccagt tgcctggata tctgataaga gttcttgtga cgttgtccat
     1801 ccaggtagcc gataaaatga atatgaagcc ctttgtctgg tgtatattcc attacccagt
     1861 aatatcccgc caacatcgtc tgggtttcgc taagtaaacg gtatatttcc atacacatac
     1921 tgtgcttaca ggaatgcccg aaactgggcg tgtctttcct gtaagcaaaa tcaattctga
     1981 aaggtaacag tttagaaaaa cgttgaaaca taccatccat gtgttcattc acgtctttca
     2041 gaatcatgaa atccattttg taattgggat tggcattata cattttatag tccttacttt
     2101 ataaaactca caggaagaga gaattacgta aatactgaga gtagagaaac gccgtaccac
     2161 ggcattaata aacgaagtaa accagcagca cagacaacct gataaaactg ttttgtatta
     2221 ccgcattact gtgatgaggt aatacttcag aatacagtga gactctctta aactgagaac
     2281 cagtacgaat tacaatggaa aaacacacct catagaattg agtttcagga agcaccacta
     2341 aattctttac aggaagtaat ctgacattca caaaactcag tatcggctag ctacaggcaa
     2401 catcgctatt aatattgtcg aaccattaag taaagccgag agagaccacg ggtatttaat
     2461 cattagcata aaagaataat ggtatacaga acatacccac acaccataag aagattgttc
     2521 catacagaaa agatcatatt tacctatacc tataaactgt aacggagtca taacacgctc
     2581 taatagtgtt ccgatacgta accagtgtta agtacgtaca ggacaacatt acacacataa
     2641 ttcaccatac agcttccccc atatgcttca ctgcgtagtg tatggagata tatgcattac
     2701 acacatacag aatacttagc ataaagagga gtgacattac cacgtaaaga gataagttat
     2761 acaacaaaca tacaaacaat acggcacaga cacacaatac cataactcca actaataata
     2821 ctaattaacc ttaattattt atggtatatg aaataaatgt gattctcccg gaactaacta
     2881 acctaaccag acagatgatc actgtataaa aacttcagtt aatactcatt tgttttgtcg
     2941 actcagtata taatgagtga ccagtaatca ggataatcac acttacataa cctgtaaaaa
     3001 agaacaagaa gaataagcca ctaacataat gcaaaatggc atctgaatcg atcgcagttt
     3061 ggcattcgaa tcgatcatga tttggcccaa tccgtgatcg aatagagcgg aatacgcaac
     3121 ataattcacc agacaagagc ttacaccgaa ttacagatta tgtattaatg tcaaaactgg
     3181 catattttac agatacaatg aacatgcaac ccatcaatct aataggggcg gtatgtacat
     3241 ctgcccatga atgggatggc gctaagaata caaaacaatt agacatagat cacaaaaaca
     3301 caccattcta attgaaaaac aaaacaaaca aataaatcat actattatag aattttctct
     3361 tgccagacat agataatacc cctgctaatt ttgctaatgg ttagcaaaat tagcagggta
     3421 tataaagatt attgacccaa aacacactca gatgaattca cgacagctca ggagggttat
     3481 tcctgatgtc atcttcacaa ataatggtta acgcatcata tatcattctg cgtctgttcc
     3541 tgttttcggc cttatcgcca tacaacatca tcacattttt aatttcatcg accggtgatt
     3601 tcacatagtg aactaataca ccaagcttct caacacattg cagtaaagta tatttcttac
     3661 tgcctgtgaa aagccctatc atagtttctt tacgttcgcg actggataat actttgtttt
     3721 ccataatgac ttctgccagc gtcttaccat cataaacata ctctttatct atcagaatat
     3781 ttatcttatc cagcaccttc tctacgtcat caagcaaacc tgtaatatat gggggcgcaa
     3841 tatatggttt cgtactgatc ttttcaagca tacaattata catcttaata acacgcatca
     3901 gggtaaaatt gttcgcatca tactgtattt caccctcctt atatacagga tcaataagtg
     3961 gtattttttc aataaatgca tcctgatagg cttttacatg gtcaagccaa taaaacaacg
     4021 agccatactt tatatttttt ctatttactt taacctccag tgctgtccga agcaacgata
     4081 atgcccccct gacatatcct gaaggtaatt caccaaacaa tccttttctt tccatgtcat
     4141 cataatgctg cagcacatca ttaattttat ttttccttat cagacaaatg tacaccgcaa
     4201 gtgtttcgta gcagctggtc acatgaacag cctcattctg caactgccca aaatagctta
     4261 taatcttttt cagatatgcg tcatcacaac cattcttatc attaatatta aatacaagat
     4321 ctttcagacg ggaaatatat tcctgagatt tatcatcaag tgaaacacta taacgagaca
     4381 atgttccttc tgcactgttt tgaaacttat aaaacatttc cagtaatttt tttgcataag
     4441 gcgttgtatc aaagctggta aagtcataaa gatattgcgc agcgtaacca tacaaaccat
     4501 gaatccagag tccttctctc accaacactc tgagataata tgcaaggctt tgaagtatct
     4561 tcgtctttcg ttgtatatca agtaagacac ttaatgaata aaatgcatta tagaatttat
     4621 caatattcct gagcactttc tccagttcat ccttatccag cttcttctcc cggtaatcct
     4681 ttttatattc atttttgata gccatgatat attgttgcat caggctcttg cttttcccgt
     4741 tatccttccg tatagttcct gtatatttcc ccagagcata cagctttcca ttttcatgaa
     4801 aatcttcctt gttgaatccc agttcagcca gatattcccg aagataacgt ttaacagcag
     4861 cttcatcgct ttctgtatca gcgtgatcaa gacaaggaca atacgatttc tgtaaaaaat
     4921 tataaatatg ataataaaaa gcagagtcat tctctggtct gtatgtatta tacatatcta
     4981 caacaagaaa aataacccca aaagtgcgca gacaatagct gcgaaggaat tcaggtttta
     5041 gtgtggaatg cactggataa gaattcatca tgtaataaaa atgttcatat cgggcatata
     5101 tgatccgccg ttgctcctga ctgaacaccc cttcagcatc aataatacga ctgatctcat
     5161 tataacgttg atcccagaat ttttgatgca tttcaaaatt ggtactcttc cgcttgtgtg
     5221 ccggattctc gccttcctta aacaacccca aaacagtaca aatatcatac aacatatttg
     5281 taaatgtgcg catatgctgt gctctccggt tggtttgcat tttatcgcag aggacattct
     5341 gagcttaacg tatcaaactg acacaacgac atgcaatgca tatggagaac aatatgtcaa
     5401 atcaaaccgg acagctttac acgccttcac gcatcctgcg tatggctgaa cttacccgac
     5461 tgctgggtat atcaagatct actatttacg tgaaaataaa tccggcctcg aaataccacg
     5521 accctacctt tcccaaacca ataaggcttg gtagtgcatc cgtaggctgg cgggcatctg
     5581 ccatcgatga gtggctgatg ttacatacgg caccagcctg gtctgaacct gaaaacaaac
     5641 taaaggaact taatgatgac gacaaaacta tcactccctg aacttgacac aactaaactg
     5701 cctgacaggt gccaggcatt acttgatgaa atgcatgaag aaacaggaat acgccgtgaa
     5761 atacttctgt ctgttatgct gacggtcatg gctgcctcgg tccaggatac gcatgaagtt
     5821 gaactttccg gagggcaacg taccagcctt cagatattta tgtgtctttc atcagcatca
     5881 ggcagtggaa aaacctctgc ctgcgcaaaa ttaatcgctc ctgttcatga aacagaagaa
     5941 gaactacatc aggcatatat cgacgataaa aaaaactatg accgaatgat ggaaatgtgg
     6001 acaacagata aaaaaatcct ggagcggaga tataaaaagg aaattgaaag atccccggaa
     6061 aatgctgctg ctgcacgagc agcacttgaa aagtgtatag caaataaacc cgaacctccc
     6121 gtacaacagg ttctcatcgt gaatgacgcg acaccagaag ggatagcttt gaaactcagc
     6181 cagtcaccct ctcttctgtt gctgtctgat gaaggaggta ctattctgga taagcgtttc
     6241 gaacgcaaat cggcactgta caacacatta tggagtgggc aaccagtcaa cgttgaacgg
     6301 gcatccagac ccgggttccg gctgaatgat gtccgtctca ctatacttat actgactcaa
     6361 ccggtaatat ttaataagtt ttttactctc actggcgatc agatccgggg caatggcttt
     6421 ctggcccggt tgctattttg cgaacccgga gcaattaaaa taatgacgac agaaccgcat
     6481 actgccactc ctgttgtgac gcaacaatgt gcaagctgtt ttcgtaagaa aagttttggc
     6541 agtcagatca gagactctct gagagcatcc agagaaagac gtgcaaaaag taaagagcgc
     6601 atctgcatga cattatcaaa tgcagcatcc cgcgcactgg agcgctttca caaagaaaat
     6661 atggatactg tcagacagaa tcctcatatg ggtacatttg aagacattat tgtcagaaaa
     6721 agagagcagg ttgtcaggat agcagcactt ctggaactgg agaaagatcc agactcgaca
     6781 gtaataacgc tggaaagtac taatagcgcc atttatctca ttgaatttta tttcaagcat
     6841 cttatatata aacttgaatc ccttcgggaa atatccccgg ctgagaaact tgataaatgg
     6901 cttcagaaaa gaattatcac aacagcaggt tacatattcc agaaaagtta tattttacaa
     6961 tatgccccat atgctctcag gaaaaaatgt gttctggatg aagcacttga catactggcg
     7021 gaacaaagga aaataaggat tgatgataac ctggttgttt atataggaaa cacgattaca
     7081 ccatccgaac tggcaaaaaa actaaatatt cctgcattcg atgccggagt tttcatttgt
     7141 gatcatcaaa acatactgaa gtatcaccga aacagactat agatactatc ggacaaaaaa
     7201 taaattatca caagtgtatt attcgttata ataatgaata atcgttatct gagtgcaatg
     7261 ccaggttttc attacgaact ggcattgcat ttatttttca aaaaagaaag cagtaaccat
     7321 ttggctactc tgagtatgcc agtccatgaa actaagcgaa ttccttagtg tttttagcaa
     7381 gttgagacat cacataatcc gcccatgcct gcatcatcgg tacacgctgc tccagtagat
     7441 cagtacgatg atatgccgcc tcaaccttat ttttcagcgt atgagcgagc gccctttccg
     7501 ccagatcccg cgaatacccc tgttcactac accagtccct gaatgttgag cgaaaaccat
     7561 gtgccgtggc aactcgcccc ggaatgtcac tgacggcttt ctttttacgc agaaaacttg
     7621 tcaacaccat atcggaaagg atctgctgct ttctgggtga agggaacacc agttcatcat
     7681 gcaggccacg tatattttcc agaatgtaaa tagcctgccg ggataaagga acacgatgct
     7741 gtagcctagc tttcattctt tctgcaggta tagtccatac ccgcttatga aaatcaattt
     7801 cagcccagcg cattcccctg gcttcgcccg agcgagttgc tgtaagtatc accattaata
     7861 acagtgcgcg ggtaacatta taaggttcat cactatacac actggtcgcc acaaaaagcg
     7921 gtaactgcct ccagggcatt gcgggttggt gttcatcacg tcctcttgtc tgctgaggaa
     7981 gcaaatggtc aaccacatca acaggatttg ccacacaaaa accgtgcgcc catccccact
     8041 gcataacaac atgaatgcgc tgtttaaccc ggcttgccgt ttctgacaag gttaaccaga
     8101 ctggacgcag tgtttctgcc acgtccgcag ccgtaatcga atccagcgtt tttgctccca
     8161 gttgaggaaa cgcgtaattc tcaagcgtcg ataaccactg ccttacatgc tttggattct
     8221 cccatccagg agacagttct gcatgtacac gcctggctgc atcggcaaat gttgggataa
     8281 cgactttctc agattcagcc tttttaatct ccagaggatc atcacctgca gcaagttgct
     8341 ctcgcattat ccgggcagta cgtgcagctt cagcaatact gacctctggg taagttccca
     8401 atccagcatt acgtcttttt tgtgtcaccg gacttacata acgaaaaacc catttccccc
     8461 gcccctttac tgaagaagga tgaagggtca gtccggtaat tcccccatgg ggcaatggtt
     8521 tgtcatcagg tttgatatgt cttgctttcg tatccgtcaa tactgccata cgctaattcc
     8581 cgtcactctg gtatgccatc cagtatgcca ttagtccctc gcttcactaa gaatacatta
     8641 gataacatcg gacaacaaaa agtgtaatac attgtttata aagaaattat ataacacccc
     8701 tggattaagt cagatttatt tcaggcggtc cccctcaccg ccatatttaa agaagagcgc
     8761 gtacgaaagt acgggctttt ttttcgtata ttgtacacac cgggtgcccc cgcccacgtc
     8821 tgttagggcg aggggaaatt gtgcacatca actgtctggt tactcagtca acaatcaaaa
     8881 tgcgcaagat agtacagaac atatatgcgt tatctggtca cttcctgtaa cttcctcact
     8941 tattaaaatg cccccaacgg aaatacatgt cacatgccat cataaaatcg ttatgccaga
     9001 ggaatcgaca atggaaaaaa tagtaaatta agcacggtac atagcagagt aaaaaatagg
     9061 cttcaaacca aatccgccat tccacaagcg ttgccaatac cgacaccagc gtaatccaca
     9121 tcatttcaat aaccagcaat atgaataacc gaagccaaag cggcttggca aaaaaggtcg
     9181 acaacgtatt ttcactggag aaaaacagat cgatttcctg caaaaaaaac ctgcccagaa
     9241 aagcgtaccg cttggagaag cgcagaaaaa acagattaat cgccaacact acgccacaaa
     9301 gaatgagcaa tgcaattatc gatttaaacg ttaaccactg cccctctgtc acaaaagaat
     9361 cactgttacg caacgggctg atagtaagca ggttaacaac cgttgagatg cacaccgaaa
     9421 gcagcacata gcgagtcaga cttttatatt ttactttttt cgctactgaa atagcgacct
     9481 gcttgacata actggtgcgg atcatccgaa acagcagaaa gatgaccaac ggcccaagta
     9541 acgaaatcag gaacagcgca tataacgagg gtagaagctg gtatagcccc caactcattg
     9601 cactcaacat gattaacgac agaaatcctg ccaccagagg ttgtgtaaga aataattcta
     9661 ttttgtggtt ctgctttttc ttaaaccaca aaaaatcgat attcttcgtg acgcatttat
     9721 aagcccagta cgccttgaga tcaaaaataa aattatacag caccagggcc agaatcccac
     9781 taactacacc aatatcctct gatggaaaag taaaccctgc atttttccaa taaaagccta
     9841 tcagaataag tccaatagca taaatgtata tcagatttcg tttatcctga aagcgaataa
     9901 aagttaaata at
//
LOCUS       DADMFL010000133         9678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.134, whole genome shotgun sequence.
ACCESSION   DADMFL010000133 DADMFL010000000
VERSION     DADMFL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.134"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            382..1500
                     /locus_tag="JNZ27_003462"
     CDS             382..1500
                     /locus_tag="JNZ27_003462"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8665660.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1566..1814
                     /locus_tag="JNZ27_003463"
     CDS             1566..1814
                     /locus_tag="JNZ27_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY8665661.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1879..2247
                     /locus_tag="JNZ27_003464"
     CDS             1879..2247
                     /locus_tag="JNZ27_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8665662.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2341..2994
                     /gene="nfsB"
                     /locus_tag="JNZ27_003465"
     CDS             2341..2994
                     /gene="nfsB"
                     /locus_tag="JNZ27_003465"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY8665663.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3102..4349
                     /locus_tag="JNZ27_003466"
     CDS             3102..4349
                     /locus_tag="JNZ27_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY8665664.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4417..5793)
                     /gene="pheP"
                     /locus_tag="JNZ27_003467"
     CDS             complement(4417..5793)
                     /gene="pheP"
                     /locus_tag="JNZ27_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY8665665.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLGSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5894..9029)
                     /gene="cusA"
                     /locus_tag="JNZ27_003468"
                     /pseudo
     CDS             complement(5894..9029)
                     /gene="cusA"
                     /locus_tag="JNZ27_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9041..9582)
                     /locus_tag="JNZ27_003469"
                     /pseudo
     CDS             complement(9041..9582)
                     /locus_tag="JNZ27_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2372 a         2686 c         2340 g         2280 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 ttttctgcat gcctcaatcg tcaaaattct tcgccaccag ccagctctgc cataacttgc
      241 cacattgaaa aagtagccaa agtgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      301 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      361 acgtcaactt aagagaaacc tatgccatta cccgattttc atttttctga accttttacc
      421 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      481 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      541 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      601 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccgcagacca tcatctggaa
      661 atttgcggcg gtggcacgcc cccgtttcag aaatggcagc gtcaggaggt atgcgacaac
      721 gaacgctatc aacgaacgct ggaaaacttt ggctatctca tccagcaggc gaccgttttt
      781 ggtcagcatg tccatgttgg ctgtgccagt ggcgatgacg ccatttattt gctgcacggc
      841 ttgtcacggt ttgtgccgca ttttattgcc ctttccgccg cgtcgccata tatgcaggga
      901 acggatacgc gttttgcccc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
      961 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tgtttcgctg tctgagttac
     1021 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     1081 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtgaatatg
     1141 gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt caaacatcag
     1201 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tggtctggaa
     1261 ggcgtcatta ccgatccgca cactggcgat cgtcgaccgc taacggaaga taccttgcga
     1321 ttgctggaaa aaatcgcccc ttcagcacat aaaattggcg catcgagcgc aattgaagca
     1381 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     1441 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa
     1501 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1561 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1621 tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
     1681 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
     1741 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     1801 cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc ttattctcct
     1861 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctagct
     1921 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     1981 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     2041 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     2101 tatcacatga ataaaaagca ctggatttcg gtgtattccg gcgaggaaat ctccgaagcg
     2161 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2221 gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt tctcgcttac
     2281 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tacttacaca tggagtcttt
     2341 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
     2401 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc
     2461 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2521 gccaaatccg ctgccggtaa ttatgtgttc aacaaacgta aaatacttga tgcctcgcac
     2581 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     2641 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     2701 cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     2761 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     2821 gcggtgccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg tctgaaagag
     2881 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     2941 gctacgctgc cgaaatctcg tctgccgcaa aacaccacct taaccgaagt gtaattctct
     3001 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     3061 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     3121 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     3181 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     3241 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     3301 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     3361 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     3421 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     3481 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     3541 tcggcgcaat cctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     3601 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     3661 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     3721 ggctggaaat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     3781 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     3841 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     3901 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     3961 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     4021 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     4081 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgacct
     4141 taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc tacgcgttta
     4201 ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     4261 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     4321 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     4381 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     4441 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     4501 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     4561 cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ccgcgcgaaa
     4621 acgcagatgc gccaggcaga tcataatcca gttcaacagc aacgttgcta ccaccagcgt
     4681 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     4741 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     4801 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     4861 cccgctgtta tacaccgaca gcgatgccac cagaatgacg aagttcagcg cagaagctac
     4921 cacattgctg ccgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     4981 cacccacgga tagagtgcca gtaaaaccac cagtgaaccg atataaaaca gcaggatgcg
     5041 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     5101 tgtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     5161 cagcccattc cggccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     5221 tttctcgccg ccgtgaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     5281 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     5341 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     5401 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     5461 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     5521 ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc accatctcgc caagctggcg
     5581 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     5641 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc
     5701 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     5761 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     5821 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     5881 gaggtatcct gctttaattc cgtacccgat gtcggtgcag ccacatcagc ttatacgccg
     5941 ccgggataat aaacagcgac agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg
     6001 caatccggct catcacctct gaaccagccc ccgttcccca cagaatcggc agcagaccgg
     6061 cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg ccgtgataca
     6121 acgcctcatc cagcttctgc tcgctgaatg tttgtggatt attcaacgac ggctcggcct
     6181 ctatggcgtg acgtaaatac atcagcatca ccacgccaaa ttcggcggcg accccggcaa
     6241 gggcaataaa gccggtaccc gtcgccacag aaagatgaaa gcccatccac cacagtaacc
     6301 agatgccgcc caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc
     6361 gacggaatgc cagatacaac agcacgaaga tgatcatcag tgtcatcggc accatgagtt
     6421 tcagcttatg gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc
     6481 ccggttttaa ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc accgacacca
     6541 tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca
     6601 gcatcgacgg tccggtagag actttaacgt cggccacgtc tgccagggtg atttgctgct
     6661 tcatcggcgt caggatcggc aactggcgca gtgcctgcgg actatcgcgc cagctttgcg
     6721 gataacgcag attaattgga taacgggcaa tcccttccac cgtttcgcca accatcgccc
     6781 cgcccaccgc agaagtcaca aacaactgca catccgccac cgtcataccg taacgtgcgg
     6841 ctttttcacg gttaatctca acgttgatat agcgcccacc ttccagccgc tcggcaagcg
     6901 cagaagctac gcctggcacc gttcgcgcca cttcttcaat ttgctcagcc atcgcgtcga
     6961 tatccgccag cacagtgccg gaaactttaa tgccgatggg gcttttaatg ccggttgaga
     7021 gcatatcgat acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca
     7081 cggtgttatc cagttcctca atgattttgt ccattgtcat gcctggccgc cactgatcct
     7141 gtggcttaag ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt
     7201 ccgctttccc ggttttgcca aatacccgcg ccacttcagg tacgctcata attagcttgt
     7261 cggttttttg cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca
     7321 tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg
     7381 gccagagaac cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca
     7441 gtactttcag caacagcgga tgataaacac gaatcaaaaa gcgattgagc ggattactgc
     7501 tttccggcgg aattttgcca cggatccagt aacccatcag gatagggatc actacgatcg
     7561 ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa cgccaacggg ccaaacagac
     7621 gcccttcctg cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac
     7681 tgataaacag cgccggcccc acttcaacag acgcatcggt gctcacctgc cagcgcgttt
     7741 tattatccag cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgcgcat
     7801 tctcgatcat gacgatagcg gcatcgacca tcgccccgac ggcaatcgca atgccaccca
     7861 gcgacataat attggcattc agcccctgga agggcatgac aataaaagca atacacaacc
     7921 ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcgc
     7981 agactaccgc caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggccc
     8041 ggtcaatgag ctggctgcgg tcgtatgttg taactatctc cacgccttcc ggcagactac
     8101 ttttcagcgt ttccagtttg tccttcacgg cggcgatcac ttctcgggcg tttttgccgg
     8161 atcgcaggat caccaccccg cccgccactt cgccttcgcc gtttaatgcc ccggcgcatc
     8221 tccgggccaa tctggtcctt cgcaacatcg cgcagataaa cgggcacgcc attttcactg
     8281 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgta gatagccgct ggcgcgtacc
     8341 atatattctg cttccgccag ttcgattgac gaaccgcccg cttcctggtt tgaagcatcc
     8401 agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg aggatcgata
     8461 accacctgat actctttcac cacaccgcct accgacgcca cttccgcaac gtcaggaatg
     8521 gttttcagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc aagatcgtgc
     8581 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
     8641 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
     8701 acccgcgaac gcgcccagta cggatcggtg ccatcttcga aaatgacata cacataagag
     8761 tcgccaaact gcgagaaacc acgcacagtc ttcgcaccag gcaccgacaa catggtggtg
     8821 gttagcggat aagtcacctg attttcaacg atttgcggtg cctggccggg atagctggtt
     8881 ttaataatca cctgcacatc ggagagatcc ggcaacgcat ccactggcgt attaatgatg
     8941 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
     9001 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca tgggtagcac
     9061 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
     9121 ggccgccgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg gtgacgcctt
     9181 gcgatgcctg gaaaacagca acgcgtttcg gtacaaagcg cccgtcggca tcaacggtaa
     9241 tcacccgctg ttcgttgccg gtatcaatca gcgcctcgtc ggcgttgtcg acttccagac
     9301 gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat ttgcggatgg
     9361 tgagtgtttt atccggtctc gccggaacgg tgagggtaaa ctgcgaggca tctttcacca
     9421 gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata ccctgaattc
     9481 gcgattacgc gcaatacccc tgagaaccgc catgaacaag ggtttcatga tttgtgattt
     9541 tagtttagta cgcaatttgg tacacaacac tattttcact tcagcgggta gtcatcaaac
     9601 tcaccataac gagcatcgtt gatgatgtat gtgatcacca caaatatcac atctggacta
     9661 gttccgtctt catgcgca
//
LOCUS       DADMFL010000134         9549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.135, whole genome shotgun sequence.
ACCESSION   DADMFL010000134 DADMFL010000000
VERSION     DADMFL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.135"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(435..641)
                     /locus_tag="JNZ27_003470"
     CDS             complement(435..641)
                     /locus_tag="JNZ27_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY8665666.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JNZ27_003471"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JNZ27_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY8665667.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2740
                     /gene="kdpA"
                     /locus_tag="JNZ27_003472"
     CDS             1043..2740
                     /gene="kdpA"
                     /locus_tag="JNZ27_003472"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY8665668.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARPLGSGLARLINDIPL
                     PGTAGVERILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQL
                     PGLSWDLALNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFI
                     RAFTRQSMSTLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGA
                     QQLLPMGPVASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCF
                     AFGEVTGDRRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESR
                     FGVLVSSLFAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMM
                     LFVLLAVFIAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGR
                     SAILNPSPHGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPV
                     MAIAGSLVSKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2763..4810
                     /gene="kdpB"
                     /locus_tag="JNZ27_003473"
                     /pseudo
     CDS             2763..4810
                     /gene="kdpB"
                     /locus_tag="JNZ27_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4819..5391
                     /locus_tag="JNZ27_003474"
     CDS             4819..5391
                     /locus_tag="JNZ27_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY8665669.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5384..8066
                     /gene="kdbD"
                     /locus_tag="JNZ27_003475"
                     /pseudo
     CDS             5384..8066
                     /gene="kdbD"
                     /locus_tag="JNZ27_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8063..8740
                     /gene="kdpE"
                     /locus_tag="JNZ27_003476"
     CDS             8063..8740
                     /gene="kdpE"
                     /locus_tag="JNZ27_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY8665670.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLDLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            9015..9119
                     /locus_tag="JNZ27_003477"
     CDS             9015..9119
                     /locus_tag="JNZ27_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY8665671.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9430..>9549
                     /locus_tag="JNZ27_003478"
     CDS             9430..>9549
                     /locus_tag="JNZ27_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8665672.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2121 a         2403 c         2647 g         2378 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgccgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa acatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttaccac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcta ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcgtc ctttaggcag
     1141 cgggctggcg cggctgatta atgacattcc tcttccaggt acagcgggcg ttgaacgtat
     1201 actctttcgt ttacctggcg tctctgacca tgagatgaac tggaagcaat atctttgtgc
     1261 cattctcggc ctgaacatgc tggggctggc ggtgctgttt tttatgttgc tcggtcagca
     1321 ctatctgccg cttaatccac agcagttgcc ggggctgtcg tgggatctgg cgctgaatac
     1381 cgccgtcagc tttgtcacga ataccaactg gcaatcttat agcggtgaaa ccacgttgag
     1441 ctatttcagc cagatggcag gcttaacggt acaaaacttt ctttctgccg ccagcgggat
     1501 tgcggtgatt tttgccttca tccgtgcgtt tactcgccag agcatgagca cactcgggaa
     1561 tgcctgggtc gatctgctac gcatcacgtt atgggtgcta gtccctgtgg cgttgttaat
     1621 cgcactgttt tttattcaac aaggtgcgct gcaaaacttt ctgccctatc aggcagtgaa
     1681 taccgttgaa ggagcgcaac agctgttacc catgggacca gtagcttctc aggaagcgat
     1741 caagatgctc ggtactaacg gcggtggctt ctttaatgcc aactcgtcgc atccgtttga
     1801 aaacccaacc gcactgacca acttcgtgca gatgctggcg atcttcttga tcccaacagc
     1861 gctgtgcttt gcctttggtg aagtgacggg cgatcgccgc caggggcgca tgttgctgtg
     1921 ggcgatgtca gtgatttttg tcatctgcgt aggcgtggtt atgtgggcag aagttcaggg
     1981 taatcctcat ctgctggcgc tcggcgcaga cagcagcatc aatatggaag gtaaagagag
     2041 ccgtttcggc gtgctggtca gtagcctgtt tgcggtcgtg actacggcgg cgtcctgtgg
     2101 cgcggtgatt gcgatgcatg attcgtttac cgctctcggc ggcatggtgc cgatgtggct
     2161 aatgcaaatt ggtgaagtgg tgttcggcgg tgtcggttct ggtctttacg gcatgatgct
     2221 gtttgtcctg ctggcggtgt ttattgccgg gctgatgatt ggtcgtacac cggaatatct
     2281 gggtaaaaaa atcgacgtac gcgagatgaa actgactgca ctggcgattc tggttactcc
     2341 aacactggta ctgatgggcg cggcgttggc gatgatgacc gacgccggac gtagcgccat
     2401 acttaaccct agcccgcatg gttttagcga agtgctgtac gccgtgtcgt ccgccgctaa
     2461 caacaacggc agcgcctttg ccggattaag cgccaactct ccgttctgga actgtttact
     2521 ggcgttctgc atgtttgtcg gtcgcttcgg ggtgattatc ccggtgatgg caattgccgg
     2581 ttcgctggtg agtaaaaaga gccaacccgc cagctccggc acgctgccaa cgcacggccc
     2641 gctgtttgtt ggcctgttaa tcggcaccgt gttgctggtt ggcgcactga cctttatccc
     2701 tgccctggcg cttggtccgg tggcggaata tctctcctga tgatattgag tgagcactga
     2761 atatgagtcg taaacaactg gcgctattcg aaccaacact tgtcgttcag gcgctgaaag
     2821 aagcggtgaa aaaattaaac ccgcaggcgc aatggcgcaa tccggtgatg tttatcgtct
     2881 ggatcggcag tctgctgacc acctgtatta gcatcgcgat ggcaagcggt gcgatgcctg
     2941 gcaatgcgct gtttagcgcg gcaattagcg gctggctgtg ggtcaccgta ctgttcgcta
     3001 atttcgccga ggcgctggca gaaggccgca gtaaagcgca ggccaacagt cttaaagggg
     3061 tgaaaaaaac tgcctttgac cgcaagctgc gtgagccgaa atatggcgct gcggcggaca
     3121 aagttcctgc cgatcaactg cgtaaaggtg acatcgtgct ggtagaagct ggcgatatta
     3181 tcccctgcga tggtgaagtc attgaaggcg gcgcatcggt cgatgaaagc gccatcaccg
     3241 gggaatcggc accggtgatc cgtgaatccg gcggcgattt tgcctccgtc accggcggca
     3301 cgcgtattct ttctgactgg ctggtgattg agtgtagcgt taaccccggt gagacatttc
     3361 tggatcggat gatcgcgatg gtggaaggcg cacagcgacg caaaacaccg aacgagattg
     3421 ccctgactat cctgctgatt gctctgacca tcgtcttttt actggcaacc gccacgctgt
     3481 ggccgttttc cgcgtggggc ggtaatgccg tgagtgtaac ggtactggtg gcgctgctgg
     3541 tctgtctgat cccaaccact attggtggcc tgttgtcagc gatcggcgtc gccgggatga
     3601 gtcggatgct gggcgcgaat gtgattgcca ccagcggacg tgcagttgaa gcggcaggtg
     3661 acgttgacgt tctgctactg gataaaaccg gcaccatcac actcggtaac cgtcaggcgt
     3721 cggagtttat ccccgcgcag ggcgtggatg aaaaaacgct ggctgacgcc gcacaactgg
     3781 cttcgctggc tgatgaaacg ccggaaggtc gcagtattgt gatcctcgcc aagcaacgtt
     3841 ttaacctgcg cgagcgcgat gtgcagtcgc tccatgccac ctttgtaccg tttactgcgc
     3901 aaagccggat gagcgggatc aacatcgaca accgcatgat ccgtaaaggt tctgtcgatg
     3961 ccattcgccg ccatattgag gctaacggcg gtcacttccc tgccgatgtt gatcaaaaag
     4021 tcgatcaggt tgcgcgtcaa ggagccacgc cgctggtggt ggtggaaggt tctcgtgtgc
     4081 tgggcgttat tgcgctgaaa gatatcgtca aaggcggtat taaagagcgc ttcgcccagc
     4141 tgcgcaaatg ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc
     4201 gatagctgcg gaagcgggtg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct
     4261 ggcattgatc cgtcagtatc aggcggaagg tcgtttggta gcgatgacca gcgacagcac
     4321 caacgatgct ccggcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggcaccca
     4381 ggcggcgaaa gaggcgggca atatggtgga tctcgactct aacccgacca agttgatcga
     4441 ggtggtgcac attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat
     4501 tgccaacgat gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacatatcc
     4561 gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc
     4621 ggtcattttc aacgccttga ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag
     4681 ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg
     4741 tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactgctaa ccgtttgtag
     4801 tctggtgtga ggtttaccat gagtggatta cgtccggcat tatcgacatt tctctttctg
     4861 ttattgatta ctggcggcgt ttacccgctg ctgaccaccg cactggggca atggtggtat
     4921 ccctggcagg ccaacggttc gttgattcgt gaaggtgata cggtgcgcgg ttcggcatta
     4981 atcgggcaaa attttaccgg caacggctat tttcatggtc gcccgtcggc aacagcagaa
     5041 atgccctata atccacaggc ttctggcggg agcaatctgg cagtcagtaa cccggagctg
     5101 gataaacaga tagccgcacg cgttgccgca ttacgggccg ctaacccgga cgccagcacg
     5161 aacgttccgg ttgaactggt gacggcgtcg gcaagcggac tggacaataa catcaccccg
     5221 caagcggcgg cctggcagat cccacgcatc gcaaaagcgc gtaatctcag cgttgaacag
     5281 ctcacgcagc tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg
     5341 gtcgtcaaca ttgtcgaact caatctggca ctggataagc ttgatgaata acgaaccctt
     5401 acgtcccgac cccgaccgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa
     5461 agttttcttc agtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca
     5521 gcgactgcgg gcgcaagggc tggatattgt ggttggtgtg gtagaaaccc atgggcggaa
     5581 agataccgcc gccatgcttg aagggctggc tattctgcct ctgaaacgcc aggcgtaccg
     5641 tgggcggcat atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcgctgat
     5701 cttaatggac gaactggcgc acagtaatgc gccaggttct cgtcatccta aacgctggca
     5761 ggatatcgaa gaactgctgg aagctggcat tgatgttttc acaaccgtca acgttcagca
     5821 tctggaaagt ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt
     5881 gcccgatcct tttttcgatg ccgccgacga cgtggtgctg gtggacttgc ccccggacga
     5941 tctgcgccag cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat
     6001 tgaacatttt ttccgcaaag gcaatctgat cgccctgcgc gaactggcac tgcgccgtac
     6061 tgccgatcgc gttgatgagc aaatgcgcgc ctggcggggg catcctggcg aagagaaagt
     6121 ctggcacacg cgcgacgcga tccttttatg catcggccat aacaccggca gcgaaaactg
     6181 gtccgcgcgg cggcgcggct ggcgtcacgg ctggggagcg tctggcacgc ggtgtatgtt
     6241 gaaccccctg ccctgcatcg cttaccggag aaaaaacgtc gggcaattct cagcgcctta
     6301 cgtctggcgc aggagctggg cgcggagacg gcaacacttt ctgatccagc agaagagaaa
     6361 gcggtagtgc gttatgcccg tgaacataat ctcggcaaga ttattctcgg tcgcccagcc
     6421 tcgcgccgct ggtggcgtcg ggaaacattt gctgaccgac tggcgcgcat cgcccccgat
     6481 ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc gcacgattaa caacgcgccg
     6541 gatagccgct catttaaaga caagtggcgg gtacagattc agggatgcgt ggttgccgcc
     6601 gcgttatgcg ccgttatcac cttaattgcc atgcagtggc tgatggcgtt tgatgccgcc
     6661 aacctggtga tgctgtatct gcttggcgtg gtggtggtgg cgctatttta tggacgctgg
     6721 ccttcggtgg ttgccaccgt cattaatgtg gtgagtttcg atctcttttt tatcgcccca
     6781 cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc
     6841 gtcgggctgg tgatcgggaa ccttactgct ggcgtgcgtt atcaggcgcg ggtagcccgt
     6901 taccgcgagc aacgcacacg gcacttatat gaaatgtcga aagctctggc ggtgggccgc
     6961 agtccgcagg acatcgccgc caccagcgaa caatttattg cctctacgtt tcatgcccgc
     7021 agtcaggtgt tgttgcccga tgacaacggt aaattgcagc cgttaacaca tccgcaagga
     7081 atgacgccat gggacgatgc catcgcgcag tggagttttg ataaaggcct gcctgcgggc
     7141 gcgggcaccg acacgttacc cggtgtaccg taccagattt tgccgctaaa aagcggcgag
     7201 aaaacctacg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg atcccggaac
     7261 agcagcgcct gctggagact tttacgctgt tagtcgccaa tgcccttgag cggctgacgc
     7321 taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     7381 tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt ggtcaggcag
     7441 aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag gccagcgaga
     7501 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     7561 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     7621 gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     7681 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     7741 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg
     7801 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca ggccaggagc
     7861 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg ggtgtcgggc
     7921 ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt accgcgttca
     7981 accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc gctcctgaac
     8041 ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     8101 tcgtagcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg aggccgaaac
     8161 tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta ttctcgatct
     8221 cgacctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcccggt
     8281 gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg cgctggatgc
     8341 cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg cccgtctgcg
     8401 cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga aattttccga
     8461 tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac
     8521 accaattgag ttccgcctgc tagcggtgct gctcaacaat gccggaaaag tactcaccca
     8581 gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc actatttgcg
     8641 tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc ctcgccactt
     8701 cattactgaa accggtattg ggtatcggtt tatgctttga attgttattc aataaattcg
     8761 cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt ggcaggttta
     8821 atttcttaac gcaaatataa aacacgaaat tacaacattt aagtaacaaa acagcatcta
     8881 atatggcgat attcataatt aatgatttat tatctggggt atgatcgttt tttgttgatc
     8941 ttcttcacag attatagcca tttcatggat agaataactc taccttcaac tgacacagca
     9001 agcggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg cggacaaccc
     9061 atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat gcccgctagt
     9121 cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat tacaggttaa
     9181 tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa taattcgagt
     9241 cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg gtctgggtta
     9301 ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt tgcttttttt
     9361 ccggcgtgat caatttctcc tttgagaaat tgaggacctg ctattacctg aaataaagag
     9421 atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga ttgtttttcc
     9481 actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc cgccattatt
     9541 ttatcagtc
//
LOCUS       DADMFL010000135         9327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.136, whole genome shotgun sequence.
ACCESSION   DADMFL010000135 DADMFL010000000
VERSION     DADMFL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.136"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            311..673
                     /locus_tag="JNZ27_003479"
     CDS             311..673
                     /locus_tag="JNZ27_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8665673.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            670..1587
                     /locus_tag="JNZ27_003480"
     CDS             670..1587
                     /locus_tag="JNZ27_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY8665674.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1600..2865
                     /locus_tag="JNZ27_003481"
     CDS             1600..2865
                     /locus_tag="JNZ27_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY8665675.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(2932..3555)
                     /locus_tag="JNZ27_003482"
     CDS             complement(2932..3555)
                     /locus_tag="JNZ27_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8665676.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4285..4644)
                     /locus_tag="JNZ27_003483"
                     /pseudo
     CDS             complement(4285..4644)
                     /locus_tag="JNZ27_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4766..5026
                     /locus_tag="JNZ27_003484"
     CDS             4766..5026
                     /locus_tag="JNZ27_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY8665677.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5044..7779)
                     /locus_tag="JNZ27_003485"
                     /pseudo
     CDS             complement(5044..7779)
                     /locus_tag="JNZ27_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7780..9111)
                     /locus_tag="JNZ27_003486"
     CDS             complement(7780..9111)
                     /locus_tag="JNZ27_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8665678.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPNVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQPPELSIDFINRYGYLPSLGQ"
     gene            complement(9121..>9327)
                     /locus_tag="JNZ27_003487"
     CDS             complement(9121..>9327)
                     /locus_tag="JNZ27_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8665679.1"
                     /translation="YANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLA
                     SLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT         2414 a         2166 c         2005 g         2742 t
ORIGIN      
        1 tacgaccttc gcattacgaa tctgtagcac caatcataac tatctgtttt agcaagcatt
       61 aaccgcattc actaagatat agttgatggc acaaacagaa agttgatgaa tgatgttgcc
      121 atatatatgt cacaaatacg gcacaacgat cttcaaacat atagccacac attcacagaa
      181 gagcacaaag ccttgcaatc cagtgcaaag atttgtgtgc cagagttttt ctcatcaact
      241 accgcaagta tcgatcgatt gagacttgga tgatagactt catgcctttg attattagct
      301 gatagaagaa atgttaaagc tatttgcaaa gtacacatct ataggcgtgc tgaacacact
      361 tatacactgg gtggtttttg gtgtatgtat ctatgccgcg cataccaatc aggctatggc
      421 aaacttcgca ggtttcgttg tggctgtgag ttttagcttc tttgcgaatg caaaattcac
      481 attcaaagca tcaactacaa cgatgcgcta catgttatat gtcgggttca tgggaacact
      541 gagtgctact gttggatggg ttgctgatag atgttcactt cctccaattg tcactcttgt
      601 taccttctcc gccatcagct tggtgtgcgg tttcgtctat tcaaagttca ttgtctttag
      661 ggatgcgaaa tgaagatatc tcttgtcgtt cctgtcttca atgaagaaga agcgatacca
      721 attttttata aaacggtacg tgaattcgaa gagttgaagc catatgaagt agaaattgtt
      781 ttcataaatg acggaagcaa agacgctacg gagtcaatca ttaacgctct ggctgtttct
      841 gatccgctag ttgttccgct gtcatttaca cgcaactttg gtaaagaacc agcattgttt
      901 gcagggttag accatacaac cggcgatgcc gtgattccaa ttgatgttga cctgcaagac
      961 ccgattgagg ttattcctca tcttattgaa aagtggcagg caggtgctga catggtgctt
     1021 gctaaacgtt cagaccgctc aactgatgga cgactgaaac gtaagacagc tgagtggttc
     1081 tataaattac acaacaaaat aagcacccca aaaatagagg aaaatgtcgg agattttcga
     1141 ctcatgtctc gtgaggttgt ggagaacatt aaactattgc ctgagcgcaa tcttttcatg
     1201 aaaggcatac tgagctgggt tggtggtcag acagatgtcg ttgaatatgt acgcacagag
     1261 cgtgttgctg gcatctcaaa atttaatggc tggaaattat ggaatctggc actggaaggt
     1321 atcacaagtt tttcaacctt tcctcttcgc gtatggactt atataggctt gtttgttgca
     1381 agcatttcat ttttatatgg tgcatggatg attatagaca ccattgtctt tggtaaccca
     1441 gtacgcgggt atccctccat gcttgtatca atacttttcc tgggtggagt gcaactgatc
     1501 gggattggtg ttctcggtga atatataggt agaatttatt tagaaacaaa atcaagacca
     1561 cggtacttaa ttaaaagcag aaaataacag gatgatctta tgaagatatt cataatgcgc
     1621 aattggcaca agatatcaat ttttatactt gcatttactt tgatttggct taggcgcatt
     1681 gacattttga caaatgccca gttttgggct gaggatgcag ttttttggta taaagatgca
     1741 tacgaacaag gttttttcag ttcactcata accccaagaa atggttattt tcaaacagta
     1801 tcaacactca ttgttggtgc gacgacattt attaatccaa tttacgcacc attattatca
     1861 aacttttttg gaataataat tagagctata attatatggt tcttatttac cgatagattt
     1921 aaattcctaa gcaccacatc aaaaatcttc atctctgtat atttaatttg catgcccggg
     1981 cttgatgaag tccaagccaa tataacaaat gctcactggt atttatcatt gtatgttgca
     2041 atgataataa tatctgaaga atccaagtca aaattatgga aagcacatga tttatttttt
     2101 atagtattat ctggattgag cggtccattt attatattca taattgcatc atctgttttt
     2161 aaatatttac acttatcaaa aggagtaata agcataagag gtttatactt attttatact
     2221 cgactgccat atttggcaat gatcttgtgt gggctgatac aggcaacatc aataattctt
     2281 acatttaatg gaacaagatc acatgctcca ttaggattca gttttgatgt tatgtcatct
     2341 ataatatcat ctaatgtatt tcttttttca tttgtccctt ggaatatcgc taatgctgga
     2401 tgggataatc accttttatc gtacacacta tcaactttag taattacatt atgtgttttc
     2461 atttatatta aaggaaattg gcaaatgaaa gtgtttgcca cccttccaat acttattgtc
     2521 gcattttcta tggcaaaacc tcaactggca gattctgttc ctcaactgcc aacacttgca
     2581 accggaaacg ggtcaaggta ttttgtaaat attcatattg caattttttc attaatatgc
     2641 gtttgtttgt ttcaatgcat aaagaataaa accttaaaaa tattctttaa aatctatgtt
     2701 tcagtcatcc ttattgcaat gataaaatta aactttttta taacccctct tcctgacatg
     2761 aactggtctc aaggagcaga attaataaat aaagcaaaac atggagaagc cgtctcattt
     2821 acagtgctcc ctccatggtt aactcttgat ttaataaaaa aataaaactt cacattaata
     2881 aataacaatg aatccgcctc cctacgtatt cccgtaggga ggctgtcgtg tttatagtag
     2941 cctggtaatg ctaaatgcaa acgaactgga gattcctgct ccgctaagcg tattgcgcag
     3001 ggtcacccta aatgtatcta caccaatata agtaacatca caactaactg aatcaggcgc
     3061 agatataggt gttatctgaa ccacataacc attagtagct gctgcgagcg gatgagaaaa
     3121 cgttacatca tacacaccag tagcatgcgc gttaacggat gaaacacccg gcgcaatgtc
     3181 tacactacca ttggcgttaa ttctccctga gcgcgtaact gactctgcag aaaccgatgt
     3241 ggccttactg gttatttcca taaaggtgca accaatatcg atcaattcgc aattgttccc
     3301 tgttatccag tatggtcgat ctgcacttgg ctgataatcc cctgcgctct ggaacgttgt
     3361 gccattgagt aagactataa cttttccccc acctgagctt gttacttgta tattcgtgtg
     3421 cgtgtagcgt tcactgctca cgcgatgaaa atttccaccg tttataacta ccgttacagg
     3481 tcgcgtccca gtattatcta tagcaaggtc tgcccccccg gcattagcct cgaagtatgg
     3541 gttgtttaac accaaagcac acgaaccgtt aaggccattt atggctaatt gcgcaccacc
     3601 tgtaccggaa tccccctgag taccatttcc ttcacaagtc aggctgttga tagtagttcc
     3661 tgccccccat ctatcaccaa gtatacccca tcgtgtattc tggcttgcag ttaatcggtt
     3721 aactgtaatg gcattaagct ctgccccttc actggagtta agtgcgcgcc agccaatacg
     3781 gttaccctgt aagtacacat tatctaactc tgacgttaac accgagtctg tacgcaaccc
     3841 ttcgccggta gtgtgcccat aagacacaat gttctctaat ttagtgtatg ctttattggt
     3901 tacgagaaga ccgtaagacg atcccgaacc tttaagtgta atgtccccca gataatcata
     3961 ggcgtgtaca cgttgggttg ccccgactgt atcaccgagc atctttaagt tgaaatccga
     4021 ggcatggcag ttaaatatcg tttctgccat attttctccg gaataatgag cacggactcc
     4081 tgtcgaacca ataaatttag cgtcatagtt gccgtaatca agaacgagtg gtgacgcgat
     4141 atcgtacacc ccttgcttta tttttaatgt tccaccgaaa ggccacgatg taagcgggtc
     4201 ttgatatatt tcatttttta ttgcaaatag ctcctcttgg agatttttgc cgctattagt
     4261 gccaatgtga gcagctcctt catctgattg aagtaactgc tttaactgat caggatcgta
     4321 cttcagcaca ttctgaaaat agaactgctg cgaaccatac gcatcatata cagccattga
     4381 atggccttgc tcagttacaa atttggcaat ctgcccgttg tacacaggat atccggcagc
     4441 attgataaca attggctgag aaacagggac gtgagatccg tcttcattct ctacataaac
     4501 ctgaatctgg ttttcaggat ttaccgggtc agtgtcaatt tttccgatat aaattttgcc
     4561 attggctacg gctttaaaag aacgcgccat agtgaagagt tgcgaaggca tgctgaccac
     4621 aacatttgcg gcgatatctg acatttcatt gctccagacg aatgatatga tgcaatcatg
     4681 atgtgattgc ataccgaaat ggcactattg aatatttatc cagtaggtta cgatgccatt
     4741 ccacccaact ggtgaggcat caaggatgta cagcaaatac gacgaggcac agtttcactt
     4801 gagacttccg catgaactcc acgcgaaaat taaacagtgt gcgaagatga ataacagttc
     4861 gctgaactca gagataattg cagcgattga agaatcattg gctaaacaaa gctctgcatc
     4921 agtttacatt gacgatgcag agcgtatggc agaacaacaa tctgatatgg ttaagaaaat
     4981 tgtctttgat acgctcaagg agctatataa aaaagacagc aactaaccat ccgtggcttt
     5041 ccgttacttc tggttatcat cactgttcat ccagtacatg atgcctagcc ttgatatggt
     5101 tttcttatct gattctggaa gtgagttgta aaacgagcgc caagcagaag agcgtcttac
     5161 agttgtatca acgatgcttt ctggtgcttg ttttactgcc agccttgctg ctttctggaa
     5221 ttctggtgag ctaattagct tttctgcaga ttcaattgat tcagcgccgc ccatcgccct
     5281 gattttgctg gcgacttttg ccccaatttc tgacccagca acagcaccta ctgcactgaa
     5341 cggtcctgac acagttgaac caaccgcagt gccaatgcgt tgggcatggt tagcaacaaa
     5401 ttcatttgcc gcagtgacgc gattaaaccg tttgacgaac tcgtttagtc ttcctgtagt
     5461 tatctcgtaa gattttgcat ctttgatagc ctttgcgacc ttatatacat cgttcagacc
     5521 tgacatagtc tcttttggta aatgtctggc aagattgcgc atctgcccgt ttgctaacat
     5581 gttttgatac cagtcagcaa accctgcagg attaaaatca gcgccacgtt ttccgttcga
     5641 aagcatatcc cgaagacctg ttcccagtag ctcggttctt agcttcctgg acggcgtgtt
     5701 ttgcatcaat tcacgaaatc ctttggcatc gcctttcgac attgcctgta gtgctgtagt
     5761 tgctttgcga gaaacatcac cgttcagcgt tctgccagtt agattaatca tttgctcttc
     5821 catgcttttt cgcatctgaa caagcctctg agcaacttca aaatcacgta atgcatttgt
     5881 ctctgacagc gccgcctttt gatcatcggc gagcgaaccg taaagcctcg ataaagcagc
     5941 gcgatcagca tctttatatg gtccagaatt cttatgaagt gcatcaccaa caagtcgtcg
     6001 ttgcttattg agatttgcat aagttaatgc gccgttcttg cctggattaa ctgcccgaaa
     6061 gactctcttt tcaatagggt caaggttttt catcccgcca agatcttctg ccacctgttt
     6121 gagcattgac cttgttgatg gcgcatcaat ttttgcacct gcaggcatcg ccttttcaac
     6181 attgcgataa agctggtcgc ttcgtttcat cagcgcagac attcttccat tgaccgtatc
     6241 aataaacttg tcgctcatac caagagcatc ttttgcaccg gaaacgcgat caattatctc
     6301 acccgcgccc tgtgctagct gcctgattgc ttcattttcc tgcacctgta gagcagaacc
     6361 cgcacgggac ttgattgctt gctcaactgc cttgtattgc tcgttcccgg aaaagtgtga
     6421 agggagtaat gaatcaacat taagcctttc agcagactcc agaacttcag cctgaggatt
     6481 gatatctagt tcatcaagcg atgaagcgag atttggcctt ttttgtgctg ctactgtgcg
     6541 aacgacttcc tctgggttct tcgtcgcagg cgtcattggt acaccttgtg ctactcttgc
     6601 ctctgcggca tgaaccgcag gatttgttgc tgcagcggca tcaccggaga atggtgatgt
     6661 ttctgttggc gcagtggtcg ctctctgtgc agatgtttta ttgccactaa ttatatttga
     6721 tgcaccacga acaccacggg taacgccatt tatcaggcca tgcgtagcaa tgctaagtcc
     6781 ggcattaata gcggcatttt cggcaaaatc accccgttga ttcgccgcgt cagcgagaga
     6841 accaatgacc atgtttccag ctacacctgc tccaggaaca agataaccgc ctattgactc
     6901 tccagcttgc gcgtaggggt ctgtcggtct gtctactgga cgataaacat catccaatac
     6961 ttttggcccc ctaagcccct gactgattgc attaatcaga cttgcgccac cctgcaatac
     7021 gtcaaatggt atgtttacca gaccacgacc agcctgttct gcaatttacc ctgcactttg
     7081 accaccagtg agccaatcgc cagcttgttg catcaatgat ggttcttccc gtgttggtgc
     7141 attattggcc tgattaactg tttgttgctg aacaggtttc ggtgcgacag aacttacggg
     7201 ttgaggtggc tgctgactgg ctgcctgctg ctcaatctga gcaaatggat tatttgggtc
     7261 tgactgcaca cctgatgccg atacttgttc ggatgactgt acttccagtt gtgcaaacgg
     7321 gttgttaggg tcttgctgag gatgtacctt tgcagaggtg gcgcgctgtt cgactgttga
     7381 gtctgtcacc gggtcaccag cccattgagc aaagcgatca tcaacgtaac cgcggccttc
     7441 aggtcctggc gtatattcac cacgctttgc cttcataacg ttgccgggac cgtcgtgata
     7501 agcctgaaga gcgtcacgcc agttaccaaa ctgctggtac atctttgcca gatagcgcgc
     7561 gccagcgtca gcctgatatt cggggttttg catttgctca tcggtataac ccatatcacg
     7621 ccatgtccca ggcatgacct gagtcaatcc tacagccccg gcggagctta ctgcggcagg
     7681 gttgtaagaa gactccttgg cacccagtgc agtcatcaac ccttctggca caccgtaacg
     7741 tgcgccagcc tgctctaaca aatcacggta attagccatt tactgcccca aagatggaag
     7801 atatccgtag cgattaatga agtcgattga cagctcgggg ggctgcttca ggtaatctat
     7861 agaagcctga ggcgcttcca ctcgcttgat accgttttgc tgaacgtact taccaaccgc
     7921 ctcattacgc ttctggttga gcgtgttcag gatgacgcca gcgttgcgac gaaaggactc
     7981 ctcgctctgc gagttctgca gcgaaccaac agcctggtcg agctttttgc cctcggcatc
     8041 agaaagtgcg cccatgcctc gcatggcctg aaccgctgtc aggtatgctt gggatttaaa
     8101 ggtatcaagt cgtgcctgag tgtctgcagc ctgtgagcct ggaacgttgg ggattactcc
     8161 acgtaagcct gtaatgctct taagtgaagg agaactaacg atatcgttca gagtgaacat
     8221 gctggttgtg agggtgttga tgccgtcttt gtagccatca tttagcgctt gctgcttctg
     8281 ctgcaactgc tggttgttgg ctgctatgcg gctctgtatt tcctggcgct tcaggtcgtt
     8341 agtttctgct gatagcatcc ggtcaaggcg cttattttcg ttgttaatgc ggtttgtttc
     8401 tgcgtccaga ttaatgcgct gctgacctaa attcgcctgg atatcttgcc cgcgcattgt
     8461 gattgcctga ttccgagcgg cggtttgcga atccaggtcc tgaccgcgca tggtaacctg
     8521 gcgaccctgc attttatcct gtaggtcaaa gtatttttcg ggtccgagac tgttcatccc
     8581 caggtgatcg acaaattcgc cgaactgccg cgggttctgt tggtacatct gagcgacgtc
     8641 ctgaggatta acaccaacac gagctaactc accggcgttg ttttgcagcc atgattgcat
     8701 tgcttctgga gacgatgacg caaggcgtgc gccagccgct aaggtgccga tagaattacg
     8761 ctggtcttca tcaatgaatc ccatgccttt acgaacggat tcaatctggt ctggatattg
     8821 agtagccaac tgacgcaaag caccgcgatc accagacgca taagcattag cgtacgcctg
     8881 ctgaaattct ttctgccgct gagcctgctt ttcctgctga aacacccccg caatacctga
     8941 aaggccttgc aaagcagtca gcccaacatt gttagcgcct gaacgctcaa tatcattgtt
     9001 ctgcctgata agctgaagcg tattgccgat gtcatttacg ttcggagcgt ttgagttgac
     9061 gccgccgata ccagccaata atccgccatt tgatccttgc caagtagcca tgattacccc
     9121 ttaaaacaac gagccaagca atccgatacc agcaccaatg ccagcgcccc aaggtgttga
     9181 tgttcccaaa aggctggcaa gacctgcacc ggcaatcgca ccagacgttc cgccactaat
     9241 tgctgtctga agacttgatg gtttgttggc attagcagcg gcaagagctg cgctttgctg
     9301 tgcaatgctg ctcatgttgt tggcgta
//
LOCUS       DADMFL010000136         9304 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.137, whole genome shotgun sequence.
ACCESSION   DADMFL010000136 DADMFL010000000
VERSION     DADMFL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.137"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            83..364
                     /locus_tag="JNZ27_003488"
     CDS             83..364
                     /locus_tag="JNZ27_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665680.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNZ27_003489"
     CDS             complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="JNZ27_003489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY8665681.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNZ27_003490"
     CDS             complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="JNZ27_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY8665682.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLSPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNZ27_003491"
     CDS             complement(3461..4831)
                     /gene="purB"
                     /locus_tag="JNZ27_003491"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY8665683.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNZ27_003492"
     CDS             complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="JNZ27_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY8665684.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNZ27_003493"
     CDS             complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="JNZ27_003493"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY8665685.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNZ27_003494"
     CDS             complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="JNZ27_003494"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY8665686.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNZ27_003495"
     CDS             complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="JNZ27_003495"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY8665687.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPD
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7968..9218
                     /gene="icd"
                     /locus_tag="JNZ27_003496"
     CDS             7968..9218
                     /gene="icd"
                     /locus_tag="JNZ27_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY8665688.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         2299 a         2342 c         2368 g         2295 t
ORIGIN      
        1 catgaggatt ttgcttttga tataagcgac agccaccgcc agaaactgga agaatttagc
       61 ggggtgtaat gaggtcagca gcatgacaga agccgaaata acgggattaa tccgccgcgt
      121 tgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagcg tgacagccga
      181 aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatagaac ttaatggcgt
      241 ggatatgcgc aacatacgaa ccagagttct tgagttgctg gcctaccgtc gccgcgtgga
      301 gatgtatcgg gagaaggaga aaatcacgta ccactggaag aagccggagc ggttacggcg
      361 gtaactcact gagattaaag caaagcgcaa aattgcgctg gctacatccc tcacatctga
      421 ggactgttaa ccagcaggcc ggaatatatt cagaaactac gaaattttcg cagtttaatt
      481 actcgtcagc ttaatcgcta acccgctctg atattttcgg aaacctcaat ttgaggaagt
      541 cggcgaggta actgtttgat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac
      601 caccacctca atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt
      661 taaccgttca ttaaaaatcg acgtgatttt gtgctttccg gatgacgtgg ccaacgtcat
      721 tttttagcga aaaattctga ttaaggtcac tggctgaacg gtcataatga ccacgcagac
      781 catcgaggca accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa
      841 ggtgcacatc tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt
      901 aaaggttaac cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcgacatta
      961 acgcccctca tgaattggtg cgcttccccc tggaaagatt acccgcctgt atatttcttg
     1021 tgtctatttg ttccacgttg tttcatacag tgcaccaaac ggtgtagtta atggtgtagt
     1081 caatttgcga tttttggcac tttttcaatg aaaattgttt aatcgaatca atgacttgat
     1141 gtagtggtaa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     1201 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     1261 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     1321 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     1381 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     1441 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     1501 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     1561 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     1621 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     1681 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     1741 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     1801 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     1861 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     1921 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     1981 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     2041 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     2101 gttaattttg gcatccgcga tgttgccggg tagacgttta ccgccaccga gtgggtcatc
     2161 tccgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     2221 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     2281 agccagtcag gctggatcat tttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     2341 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     2401 aactcgacat gcaacttatt gttttcccac tgcgcaaggg tatagaacag attgctctcg
     2461 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     2521 attccgtagg caagcgaaag caccagtact accgctgccg ttgccaacag aaaacgtacc
     2581 cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaggt
     2641 agccctggcc gcgaacggtg gtaatgactt cttgcggata ttgtgcctga atttttttgc
     2701 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     2761 gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc aacgtttcca
     2821 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     2881 gagagagatc aacctgaaac ggggagagcg aaatgacctg tgaagccaga ccgctattac
     2941 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     3001 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     3061 ataccagaat cggcagtgaa acatcgttgc tacgccagcg gcgaatcagt gacagaccgt
     3121 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     3181 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     3241 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     3301 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     3361 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata atcttaataa
     3421 gcaggccgga cagcatcgcc atccggcact gatttgaggc ttatttcagc tcatcaacca
     3481 tcgtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg gctttctctt
     3541 cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca acgcgcttac
     3601 cgcgagtaag ctctttcagc ttctcgtacg gtttttcgat gccatagcga cgcataactg
     3661 tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc agatggtcac
     3721 ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc agcgcataac
     3781 cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc tgccagcggg
     3841 aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc agattccctt
     3901 cggagttttc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa ccaatctcac
     3961 cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg tcacggtcaa
     4021 agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt
     4081 gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg acgaactctt
     4141 cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta tagttaccga
     4201 ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtgc tggcgctcca
     4261 tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct ggctgaccgt
     4321 gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta atgccatcaa
     4381 tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc attaatgcgt
     4441 gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt
     4501 gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct ttaacgtcgt
     4561 ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg aaattggcga
     4621 caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg
     4681 cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat ttcagcaaac
     4741 catattcgct gaaaatcccg cgcagcgcgc tgagtttatc gccgtagcgt ccatcgacag
     4801 gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt aaatgagcaa
     4861 gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgt agacgtccgc
     4921 cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt
     4981 tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga atgcgcgggc
     5041 caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc gtttcggact
     5101 gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc agagtgtcga
     5161 gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt gtgtagcggg
     5221 ttaattcggc gtttaagccc tggcaactgc tggcattgag cacgccgagc agggtttcca
     5281 gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag ctggggttca
     5341 tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc ccctggtgag
     5401 cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg gtgatgtcat
     5461 agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata atcagaccgg
     5521 cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt tatagaagac
     5581 ggcagactgg cccggcgtca cggcggcaac cggctcatcg aaaatcactt taatgcgatc
     5641 gtcgtccagc gccttgacgg tgcacggaat gtcggtctgg cgatagcggg tttttaccgt
     5701 gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcagctgct gggcaatcaa
     5761 cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa tgttgttttc
     5821 gacgtctttg tccaccacat accacggttc ttcggtgcct tctttggtgc caccgatacc
     5881 cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac caatttcatc
     5941 gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca ggaactcgcg
     6001 gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg cggtgaccag
     6061 accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga ccgggaacag
     6121 gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt ctttattgct
     6181 gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta cgtaatgacc
     6241 ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg ctttaaattt
     6301 gatctccttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat attcggcaag
     6361 gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt gcagttcaat
     6421 gccgagcttg tcgcagaccg cctgggcatc agccagatcc gctgccgctg tgcaatattc
     6481 ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcgacctgat atccctgttg
     6541 ttgcaacagc caagcagaaa cggaggaatc gacaccgccg gacatgccga cgattacttt
     6601 ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg cgtattctat
     6661 cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc aatcatctcc
     6721 agcggataac gttgcccgct ttgataacaa cgaatacttt ccgccaccag cggcgaacga
     6781 agatttgacg cctgtaaaat ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg
     6841 tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa acgcaaaaac
     6901 ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg
     6961 atgccggttt cttcccacag ttcacgggcg gcggcttcca ctaaggtttc atcggcttcc
     7021 agatgcccgg caggttggtt ccataacgct ttaccattaa tcgtctcttc aacgactaaa
     7081 aatttgcctt ctgcatgcac cacgcaagca acggtaacgt gcggtttaaa cattttgcat
     7141 ccttaatctg tcacttctcg ccattcgcca ttggcaagat tatccaaaga gtaatctccc
     7201 atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc
     7261 tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat acttttgcgt
     7321 tcacgaattg gtggattccg cggccataac cacgcgggtt cgtcaaccag ctccgcgcct
     7381 gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc aagtgcgtct
     7441 tgtgtgggaa taccttccac ctgcacataa tagatttttc cggtgcgttt atccggctgg
     7501 gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gcaacccttc gctatcgcgg
     7561 tcaaggcgac ctgctgcata aacgccctga accgggatga attcttttaa tgttttgcgt
     7621 ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaagag gatcacacgc
     7681 gtgggctggt tttcatgttt acgcctggta gaacgttgcg agctgaatcg cttaacctgg
     7741 tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg ccgcattata
     7801 gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac aagtcaatta
     7861 caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa acgcatatgc
     7921 aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag
     7981 tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc aacgttcctg
     8041 aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta accccagcca
     8101 tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt aaaatctcct
     8161 ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggat gtctggctgc
     8221 ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc cattaaaggc ccgctgacca
     8281 ctccggttgg tggcggtatt cgttctctca acgttgccct gcgtcaggaa ctggatctct
     8341 acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt aaacaccctg
     8401 aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg ggtatcgaat
     8461 ggaaagctga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa gagatgggcg
     8521 tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat taagccgtgt tctgaagaag
     8581 gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat cgtgactctg
     8641 tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt aaagactggg
     8701 gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc ccgtggctga
     8761 aagttaaaaa cccgaatacc ggcaaagaga tcgtcataaa agacgtgatt gctgatgcat
     8821 tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt atgaacctga
     8881 acggtgacta catttctgac gctctggcag cgcaggttgg cggtatcggt atcgcccctg
     8941 gagcaaacat cggtgacgaa tgcgccctgt ttgaagccac ccacggtact gcgccgaaat
     9001 acgccggtca ggacaaagta aaccctggct ctattattct ctccgctgag atgatgttac
     9061 gccatatggg ctggactgaa gccgcagacc tgattgttaa aggtatggaa ggcgcgatca
     9121 acgccaagac cgtaacctat gacttcgagc gtctgatgga aggcgctaaa ctgctgaaat
     9181 gttcagagtt tggtgatgcg atcatcaaga atatgtaatc actacatgtg ttaaatattt
     9241 aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat agcatatcga
     9301 gcat
//
LOCUS       DADMFL010000137         9239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.138, whole genome shotgun sequence.
ACCESSION   DADMFL010000137 DADMFL010000000
VERSION     DADMFL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.138"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JNZ27_003497"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JNZ27_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY8665689.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JNZ27_003498"
     CDS             1828..3147
                     /locus_tag="JNZ27_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY8665690.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3512..4657
                     /locus_tag="JNZ27_003499"
     CDS             3512..4657
                     /locus_tag="JNZ27_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8665691.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="JNZ27_003500"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="JNZ27_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="JNZ27_003501"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="JNZ27_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6698
                     /locus_tag="JNZ27_003502"
                     /pseudo
     CDS             5803..6698
                     /locus_tag="JNZ27_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            6695..7681
                     /locus_tag="JNZ27_003503"
     CDS             6695..7681
                     /locus_tag="JNZ27_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8665692.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7674..8600
                     /locus_tag="JNZ27_003504"
                     /pseudo
     CDS             7674..8600
                     /locus_tag="JNZ27_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8656..9087)
                     /gene="osmC"
                     /locus_tag="JNZ27_003505"
     CDS             complement(8656..9087)
                     /gene="osmC"
                     /locus_tag="JNZ27_003505"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY8665693.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2255 a         2256 c         2320 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccc cacgttggga tttattggcc tgggtgctca acaaccgagt gctgaatggg
     6541 gcgcgatggt agcgaatggt cgcaactatg tactcgatca gtggtggtat tgcgcatttc
     6601 cgggggcagc gattttgctt accgccgtcg ggtttaatct ctttggtgat ggtattcgcg
     6661 atctgcttga cccgaaagca ggaggaaagc agtcatgacc caacccgttc tggacattca
     6721 acagctgcat ttgagtttcc ccggttttaa cggtgatgtt cacgcgctca acaatgtgtc
     6781 cttgcagatt aaccgcggcg aaattgtcgg tctggtggga gaatccggct caggtaaatc
     6841 agtcaccgca atgctgatta tgcgtctgct accgacgggc agttattgcg tacatcgggg
     6901 gcagatttca ctgctgggag aagatgttct taacgctcgg gaaaaacagc ttcgtcagtg
     6961 gcgcggcgca cgagtggcga tgatctttca ggaaccgatg accgccctta atccgacacg
     7021 tcgaataggt cttcagatga tggacgtaat ccgccatcat caaccaataa gtcgtcggga
     7081 agccagagct aaagcgattg ccctgctgga agagatgcaa atcccggatg ccgtggaagt
     7141 tatgtcgcgc tatccgtttg agctttcagg tggtatgcgc cagcgggtaa tgattgcgct
     7201 ggcattctcc tgcgagccgc aattgattat tgccgacgaa ccgacgacgg cgctggacgt
     7261 cacagtacag ttgcaggtac tgcgtttgct taaacataaa gcacgcgcca gtggaactgc
     7321 ggtactgttc atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata gcgtttacgt
     7381 gatgtatgcc ggaagcgtaa ttgaaagcgg cgggacggca gacgttatcc atcatccccg
     7441 gcatccgtat accattggtt tgctgcaatg cgcaccagaa catggagtac cacgccagcc
     7501 attacccgcc attccaggga cggtaccaaa cctcacccat ttgcctgacg gctgcgcttt
     7561 tcgcgatcgt tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc tgacagcgtg
     7621 tggtgacaac aaccagcact gcgcctgttg gtatccgcag caggaggtca taagtgtctg
     7681 acacgttatt aacgttacgc gacgtccata tcaattttcc ggcccgtaaa aactggcttg
     7741 gtaaatctac ggaacatgtt catgccatta atggtattga tttacagatc cgccgtggtg
     7801 aaaccttagg gatcgtcggc gagtcaggct gcggcaaaag caccctcgca cagcttttaa
     7861 tgggcatgct gcaaccgagc cacgggcagt acatccgttc aggctcacaa cgcattatgc
     7921 agatggtgtt tcaggacccg ctctcttcgc ttaatccgcg cttaccggtg tggcgcatca
     7981 tcacagaatc gctctggata gctaagcgta gtagtgaaca acagcggcga gcgttggcag
     8041 aggagttggc tgtgcaggtg ggtattcgtc cggagtatct cgaccgcctg cctcatgcgt
     8101 tctccggcgg gcagcggcaa cgcatcgcca ttgccagagc actctcttcg cagcctgacg
     8161 tgattgtgct tgatgagcca acctcggcgc tggatatctc cgtgcaggcg cagatcctca
     8221 atttactggt aacgctacag gaaaatcacg ggctgactta tgtgctgatt tcacacaatg
     8281 tctcggtgat acgtcatatg agcgatcggg tggcggtgat gtatctcggg cagattgtag
     8341 aactgggcga cgcgcaacag gtgttgactg cttcggcaca tccatacacc cgattattgc
     8401 tggattccct ccccgccatt gataaaccgc tggaggaaga atgagcatta cgtaaaacgg
     8461 agctgccggg aaaccgcacg ttgccgcaag gctgtttttt ctacgaacgt tgccctctgg
     8521 caacccacgg atgtgaagtc cggcaatcat tagcaataag ggaggacgga cgtgagctcc
     8581 ggtgctggcg ggcgctgtag agatgctaac aacgcatcag gcattcacgc gcctgacgcg
     8641 tcatccggca atgctttacg atttcaactg gtaatccagc gtaatttccg ctttcagtac
     8701 ctgagagacc gggcatcctg cttttgcttt ctggatgatg ccgtcaaagg tagaggcatc
     8761 aatacccggc accgcaactt cactcttcag tgcgattttc gtaatcgcaa aaccggcatc
     8821 cactttatcc agcgacacat cggcggtggt atcaattgat gttggcgtga atcccgcttc
     8881 ccccagcatt aatgaaagcg ccattgagaa acatgcggca tgcgctgcgc caatcagttc
     8941 ttcagggttg gttccttttt cgccttcaaa acgcgtgtta aatccatacg gctgttggtt
     9001 cagcacgccg ctctcggtgg atactgttcc cttcccgcgt ttgatatcgc cttcccagtg
     9061 tgcctgacct ttcttatgga ttgtcattgt tgctctcctg tgggcttaaa aaggaaatta
     9121 tagactacgt gagaaacagc acgaggataa gcaggatatt ccgaataaaa tacggcgaaa
     9181 atattacttc tgcacttaaa ttaaattccc acaatagata accgggaata tatatctca
//
LOCUS       DADMFL010000138         9190 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.139, whole genome shotgun sequence.
ACCESSION   DADMFL010000138 DADMFL010000000
VERSION     DADMFL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.139"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNZ27_003506"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNZ27_003506"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY8665694.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNZ27_003507"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNZ27_003507"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY8665695.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JNZ27_003508"
     CDS             complement(3121..3420)
                     /locus_tag="JNZ27_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665696.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JNZ27_003509"
     CDS             3595..4590
                     /locus_tag="JNZ27_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8665697.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JNZ27_003510"
     CDS             complement(4632..5069)
                     /locus_tag="JNZ27_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY8665698.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPATSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JNZ27_003511"
     CDS             complement(5115..5456)
                     /locus_tag="JNZ27_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665699.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNZ27_003512"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNZ27_003512"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY8665700.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNZ27_003513"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNZ27_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..>9190
                     /locus_tag="JNZ27_003514"
     CDS             8838..>9190
                     /locus_tag="JNZ27_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAY8665701.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMIN"
BASE COUNT         2294 a         2408 c         2182 g         2306 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggcc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga
//
LOCUS       DADMFL010000139         9149 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.140, whole genome shotgun sequence.
ACCESSION   DADMFL010000139 DADMFL010000000
VERSION     DADMFL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.140"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JNZ27_003515"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JNZ27_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY8665702.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPINHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JNZ27_003516"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JNZ27_003516"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY8665703.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JNZ27_003517"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JNZ27_003517"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY8665704.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="JNZ27_003518"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="JNZ27_003518"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY8665705.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5552
                     /gene="dprA"
                     /locus_tag="JNZ27_003519"
     CDS             4449..5552
                     /gene="dprA"
                     /locus_tag="JNZ27_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY8665706.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLASWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVNGVSIAVL
                     GNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVV
                     EAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENL
                     QFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQPVPEVVTQ
                     LLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5524..5997
                     /locus_tag="JNZ27_003520"
     CDS             5524..5997
                     /locus_tag="JNZ27_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY8665707.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6026..6568
                     /locus_tag="JNZ27_003521"
     CDS             6026..6568
                     /locus_tag="JNZ27_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY8665708.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6573..7145
                     /gene="tsaC"
                     /locus_tag="JNZ27_003522"
     CDS             6573..7145
                     /gene="tsaC"
                     /locus_tag="JNZ27_003522"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY8665709.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7150..7968
                     /gene="aroE"
                     /locus_tag="JNZ27_003523"
     CDS             7150..7968
                     /gene="aroE"
                     /locus_tag="JNZ27_003523"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY8665710.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7965..8222
                     /locus_tag="JNZ27_003524"
     CDS             7965..8222
                     /locus_tag="JNZ27_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY8665711.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8198..8752)
                     /locus_tag="JNZ27_003525"
     CDS             complement(8198..8752)
                     /locus_tag="JNZ27_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY8665712.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2303 a         2221 c         2272 g         2353 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgattaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaacg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaac gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc taggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatgacaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc ctcatggtat ggcgagcgat
     4801 gggggcgatt attttgcgaa actctggcga cgtgtggagt gacaattacg agtggactgg
     4861 cgcgtggaat cgatggtgtg gtgcataaag cggccttaca ggtaaatggc gtcagcattg
     4921 ctgtattggg gaatggactt aatatcattc atccgcgtcg ccatgcccga ctggctgcca
     4981 gtctgtttga acagggtggc gctctcgtct cggaatttcc cctcgatgtt ccaccccttg
     5041 cttacaattt cccacgaaga aatcgcatta tcagtggtct aagtaaaggt gtactggtgg
     5101 tggaagcggc tttgcgcagt ggttcgctgg tgacagcacg ttgtgcgctt gagcaggggc
     5161 gtgaagtttt tgccttgcca ggtccaatag ggaatccggg aagcgaaggg cctcactggt
     5221 taataaaaca aggtgcgatt cttgtgacgg aaccggaaga aattctggaa aacttgcaat
     5281 ttggattgca ctggttgcca gacgcccctg aaaattcatt ttattcacca gatcagcaag
     5341 acgtggcatt gccatttcct gagctcctgg ctaacgtagg agatgaggta acacctgttg
     5401 acgtcgtcgc tgaacgtgcc ggccaacctg tgccagaggt agttactcaa ctactcgaac
     5461 tggagttagc aggatggatc gcagctgtac ccggcggcta tgtccgattg aggagggcat
     5521 gccatgttcg acgtactaat gtatttgttt gaaacctata ttcacactga agctgagttg
     5581 cgtgtggatc aagacaaact tgaacaggat cttaccgacg caggttttga gcgagaagat
     5641 atctacaatg ccctgctatg gctggaaaaa cttgctgatt atcaggaagg gttggcagaa
     5701 ccgatgcaac tggcctcgga tcctctctcc atgcgtattt atacaccgga agagtgtgaa
     5761 cgactggatg ccagctgccg tgggtttctg cttttccttg agcagattca ggtgctcaac
     5821 cttgaaactc gtgaaatggt gatagagcga gtgctcgcgc tggataacgc agagttcgaa
     5881 ctggatgatc tgaaatgggt gatcctgatg gtgttgttca acatcccggg ctgcgaaaat
     5941 gcgtaccagc aaatggaaga attactcttt gaagtgaatg aaggtatgct gcattaatca
     6001 tttctttaat tcagcataag ttgttatggc aaaatcagca ctgttcacgg tgcgtaataa
     6061 tgagtcctgc ccaaagtgcg gggctgaact ggttattcga tccgggaaac acggtccgtt
     6121 tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga
     6181 tggacatatt gtcaaagttc tggaggggca ggtttgcccc gtatgtggcg caaatctggt
     6241 attacgccag ggacgctttg gtatgtttat tggttgcagt aactaccctg aatgcgaaca
     6301 taccgaactt atcgataaac cggacgaaac agcaattaca tgcccccaat gtcggacggg
     6361 ccatctggtc cagcgccgct cccgttatgg caaaacattt cactcctgtg atcgctaccc
     6421 ggagtgtcaa tttgccatta acttcaaacc aatagctgga gaatgccctg agtgtcatta
     6481 tccgctactc atcgaaaaga aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca
     6541 atgtggaaag ccggtttcgg cggaataata acgtgaataa taacctgcaa ggagacgcta
     6601 tcgcagctgc gatagatgtc ctcaatgaaa aacgtgtcat cgcctatcca acggaagccg
     6661 ttttcggtgt cgggtgcgat cctgatagcg aaacagcagt gatgagactg ttggagttaa
     6721 aacagcgtcc ggttgataag gggctgattt taatcgcggc aaattacgag cagcttaaac
     6781 cctatattga tgacaccatg ttgactgacg tgcagcgtga aaccattttt tcctgctggc
     6841 caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc
     6901 gctttgattc gcttgctgta cgagtcaccg accatccgtt ggtggttgct ttgtgccagg
     6961 cttatggtaa accgctggtt tctaccagtg ccaacttgag tggattgcca ccttgtcgaa
     7021 cagtagacga agttcgcgca caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg
     7081 gggggcgttt aaatccttca gaaatccgcg atgccctgac gggtgaactg tttcgacagg
     7141 ggtaacataa tggaaaccta tgctgttttt ggtaatccga tagcccacag caaatcgcca
     7201 ttcattcatc agcaatttgc tcagcaactg aatattgaac atccctatgg gcgcgtgttg
     7261 gcacccatca atgatttcat caacacactg aacgctttct ttcgtgctgg tggtaaaggt
     7321 gcgaatgtga cggtgccttt taaagaagag gcttttgcca gagcggatga gcttactgaa
     7381 cgggcagcgt tggctggtgc agttaatacc ctcaagcggt tagaagatgg acgcctgctg
     7441 ggtgacaata ccgatggtgt aggcttgtta agcgatctgg aacgtctgtc ttttatccgc
     7501 cctggtttac gtattctgct tatcggcgct ggtggagcat ctcgcggcgt actactgcca
     7561 cttctttccc tggactgtgc ggtgacaatc actaatcgga cggtatcccg cgcggaagag
     7621 ttggctaaat tgtttgcgca cactggcagt attcaggcgt tgggtatgga cgaactggaa
     7681 ggtcatgagt ttgatctcat tattaatgca acatccagtg gcatcagtgg tgatattccg
     7741 gcgatcccgt catcgctcat tcatccaggc atttattgct atgacatgtt ctatcagaaa
     7801 ggaaaaactc cttttctggc atggtgtgag cagcgaggct caaagcgtaa tgctgatggt
     7861 ttaggaatgc tggtggcaca ggcggctcat gcctttcttc tctggcacgg tgttctgcct
     7921 gacgtagaac cagttataaa gcaattgcag gaggaattgt ccgcgtgaat caggccatcc
     7981 agtttccgga cagggaagag tgggacgaga ataaaaaatg tgtatgtttt cccgctctcg
     8041 tgaatggtat gcaactgaca tgcgcgatct ctggcgagag tctggcgtat cgctttactg
     8101 gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca tcgctgggac ctggaagaag
     8161 aagcggaaaa cttaattcag gaacaaagtg aagatgatca aggctgggtc tggttaccct
     8221 gatccagata ttcgtccttc catttcacgt aattattcgc ggaatagcgt aacccagcct
     8281 tctcttcatc acttaacggg cggatctgtt tgacggggct accgagatac agatatccgc
     8341 tctccagccg tttattttgt gggaccagac tacccgcacc aatcatcaca tcatcttcta
     8401 ctattgcgcc atcaagtaaa attgagccca tcccaaccaa aactcgattg ccaatggtgc
     8461 agccgtggag catcaccttg tgaccaacag tgacatcttc gccaatggtt aatgggttgc
     8521 catctgggtt gtacgaggat ttatgagtga catgcaacat actgccatcc tggatattgg
     8581 tgcgtgctcc gatctgtaca taatgtacat ctccacgaat cacaacgagc ggccagatcc
     8641 ccacatcatc agccagacga acgtcaccaa tcacgacact gctatcgtcg atcattacgc
     8701 gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa aacatcagac atacttacct
     8761 cagcaataaa tgatttacta atgactttgg gggcattatt ggccttgtgc aagtctttta
     8821 gtatgcaaaa aagcaccgtt ttgtgtgcga ttgcagcaaa aagggtgaaa aaacaacaaa
     8881 cagaaaaaaa gatcaaaaaa atacttgtgc aaaatattgg gatccctata atgcgcctcc
     8941 gttgagacga caacgtgaaa cacttcacag gatggtcgga acaacgaaga gaaaaaatcc
     9001 tgaaattcag ggttgactct gaaagaggaa agcgtaatat acgccacctc gcgacagtga
     9061 gctgaaagcc gcgtcgcaac tgctctttaa caatttatca gacaatctgt gtgtgcactc
     9121 gaagatacgg attcttaacg tcgcaagac
//
LOCUS       DADMFL010000140         9071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.141, whole genome shotgun sequence.
ACCESSION   DADMFL010000140 DADMFL010000000
VERSION     DADMFL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.141"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..924
                     /locus_tag="JNZ27_003526"
                     /pseudo
     CDS             1..924
                     /locus_tag="JNZ27_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="JNZ27_003527"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="JNZ27_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY8665713.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNZ27_003528"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNZ27_003528"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY8665714.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEEIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="JNZ27_003529"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="JNZ27_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY8665715.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="JNZ27_003530"
     CDS             4447..4806
                     /locus_tag="JNZ27_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY8665716.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNZ27_003531"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNZ27_003531"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY8665717.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="JNZ27_003532"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="JNZ27_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY8665718.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="JNZ27_003533"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="JNZ27_003533"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8665719.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2071 a         2201 c         2442 g         2357 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat ctcctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctagcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtaaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc g
//
LOCUS       DADMFL010000141         9041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.142, whole genome shotgun sequence.
ACCESSION   DADMFL010000141 DADMFL010000000
VERSION     DADMFL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.142"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNZ27_003534"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNZ27_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY8665720.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNZ27_003535"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNZ27_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNZ27_003536"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNZ27_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY8665721.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="JNZ27_003537"
     CDS             complement(2403..2606)
                     /locus_tag="JNZ27_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665722.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="JNZ27_003538"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="JNZ27_003538"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8665723.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JNZ27_003539"
     CDS             complement(3802..4014)
                     /locus_tag="JNZ27_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY8665724.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="JNZ27_003540"
     CDS             4274..4582
                     /locus_tag="JNZ27_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY8665725.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNZ27_003541"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNZ27_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY8665726.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNZ27_003542"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNZ27_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY8665727.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="JNZ27_003543"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="JNZ27_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY8665728.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="JNZ27_003544"
     CDS             complement(8478..8981)
                     /locus_tag="JNZ27_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8665729.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2239 a         2314 c         2150 g         2338 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat t
//
LOCUS       DADMFL010000142         9017 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.143, whole genome shotgun sequence.
ACCESSION   DADMFL010000142 DADMFL010000000
VERSION     DADMFL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.143"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(61..1518)
                     /gene="pepD"
                     /locus_tag="JNZ27_003545"
     CDS             complement(61..1518)
                     /gene="pepD"
                     /locus_tag="JNZ27_003545"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8665730.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1780..2238
                     /gene="gpt"
                     /locus_tag="JNZ27_003546"
     CDS             1780..2238
                     /gene="gpt"
                     /locus_tag="JNZ27_003546"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8665731.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2330..3574
                     /gene="frsA"
                     /locus_tag="JNZ27_003547"
     CDS             2330..3574
                     /gene="frsA"
                     /locus_tag="JNZ27_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8665732.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3632..4033
                     /locus_tag="JNZ27_003548"
     CDS             3632..4033
                     /locus_tag="JNZ27_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY8665733.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4072..5127)
                     /gene="phoE"
                     /locus_tag="JNZ27_003549"
     CDS             complement(4072..5127)
                     /gene="phoE"
                     /locus_tag="JNZ27_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY8665734.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            5415..6518
                     /gene="proB"
                     /locus_tag="JNZ27_003550"
     CDS             5415..6518
                     /gene="proB"
                     /locus_tag="JNZ27_003550"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY8665735.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6530..7783
                     /gene="proA"
                     /locus_tag="JNZ27_003551"
     CDS             6530..7783
                     /gene="proA"
                     /locus_tag="JNZ27_003551"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY8665736.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            7898..7973
                     /locus_tag="JNZ27_003552"
     tRNA            7898..7973
                     /locus_tag="JNZ27_003552"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7931..7933,aa:Thr,seq:cgt)
     gene            complement(7988..>9017)
                     /locus_tag="JNZ27_003553"
     CDS             complement(7988..>9017)
                     /locus_tag="JNZ27_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8665737.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT         2144 a         2268 c         2306 g         2299 t
ORIGIN      
        1 ataatgaagg atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa
       61 ttacttcgcc ggaatttctt tcagcagttc agtcagcagt gtccagtaat gacctacgct
      121 ttcgatgtga acttgctcat ccggagagtg tggaccggtg atagttggcc cgatagaaac
      181 catgtccatt tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat
      241 ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca gatgcatcac
      301 cggagaatta gcgtccggct gccagccagg atatgcgcct ttcgcttcgg ttttcgcgcc
      361 agccagttta cccagcgaat ccagcatgct caccacgtag tctttaccgc tgtcgatcag
      421 tgaacggatc aggcagtgaa tttctacgtt attgtcagtc atggtcacca caccgacgtt
      481 cagggaggtt tcgaccacac cttttgccac gtcggaattg cggatcacac cgttcggggt
      541 ggcgttcagc agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt
      601 cgctacagag tccagcaaca aggccagatt tttctctttc tctgccagct cgtttttcag
      661 gatctcctga taggtattca ccagagattt cagtgcgtcg actttatcag ctgtgacagc
      721 aatggtcgca aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcgat
      781 aaggcgcagg tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc
      841 attacccagg ccaacgtgga tttcaccgcc ggagtgaccg cctttcagac cttttaaggt
      901 taacttgaag gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt
      961 gaagtcgata ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt
     1021 aatcagaata tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc
     1081 ttcttcggtc atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag
     1141 aaccgccagt gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc
     1201 tttaacccat tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt
     1261 gtcgttattt ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt
     1321 ttccatacct gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac
     1381 atggaaacct ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata
     1441 ggacgggtga ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa
     1501 ttgagacagt tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg
     1561 tcacagggtt agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtattgaga
     1621 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     1681 atactggttt ttagtgcgcc agatatctat aatctcgcgc aacctatttt cccctcgaac
     1741 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     1801 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     1861 caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggggc gttactggcg
     1921 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     1981 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     2041 gatgacctgg tggataccgg tggtactgct gttgcgattc gtgaaatgta tccaaaagcg
     2101 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     2161 gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg
     2221 ccaatctccg gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt tctttttaac
     2281 ttcaggcagg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     2341 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     2401 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat
     2461 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg
     2521 tgaaatcctc gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga
     2581 tttatacgat accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca
     2641 ggcgatggtg tggcaacaaa aagcctgtac ggaagaagat ccgcaactca gtggtcgaca
     2701 ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga
     2761 cctggccgag caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct
     2821 gccgggcacg atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt
     2881 tttgcatatg ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga
     2941 tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc
     3001 gatgctgact attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga
     3061 ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca
     3121 cactcgcgtc gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtttggcata
     3181 ccttgaatcg ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt
     3241 gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg
     3301 tttggggatg catgatgctt ccgacgaagc gttacgcgtg gagctgaatc gctattcatt
     3361 aaaagtgcaa ggattgctgg ggcgtcgctg cccaacgcca atgttatcag gctactggaa
     3421 gaacgatccg ttcagcccgg aagaggactc acgcttaatc acctcatcat ctgctgacgg
     3481 taaattatta gagatcccat ttaacccggt gtatcggaat tttgacaaag gtcttcagga
     3541 aatcaccggc tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg
     3601 taaaacagtt gcatcacaac aggagatagc aatgacgtta ccgagtggac acccgaagag
     3661 cagattgatc aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga
     3721 taatcgattc tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt
     3781 gcgtgaattc tggggctggt ggatggagct ggaagcgcag gaatcccgtt ttacctacag
     3841 ctaccagttt ggtctgttcg ataaagcagg cgactggaag agtgttccgg taaaagatac
     3901 tgaagtggtt gaacgactgg agcacaccct gcgtgagttt cacgagaagc tgcgtgaact
     3961 gctgacgacg ctgaatctga agctggaacc ggcggatgat tttcgtgatg aaccggtgaa
     4021 gttaacggcg tgagtgaaat gtgccggatg catcacatcc ggcaatattc attaaaactg
     4081 atacgtcatc ccaaccgcga cagtatcatc attattaata ttcagcttat tatcgctatc
     4141 cagtttgttg attttataat caacaaacgc tgacatattt ttgttgaaat aatacgtagc
     4201 accgacgtcg atataattga ccagatcttc atcaccgatg ccctcaatat ctttcccttt
     4261 cgataagaca taacccagcg atggacgcag accaaagtca aactggtatt gagcgaccgc
     4321 ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc
     4381 agaatagaaa gttgccagat aaatattatt ggcatcgtat ttcagacctg ttgcccatgc
     4441 ttctgcacgc ttgcctgtgc cacggctttg caggttctgc tcgttggtgc gatctgagtt
     4501 ggtataggcc ccactaatgg cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc
     4561 gaagccatcg ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag
     4621 ggttaagttc aggccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc
     4681 gctggcgcgt ttggtcataa agttgtcggt ctgcgccgag gaatcgccac caaattccgg
     4741 gaacatatcg gtccaggctt ccacgtcata caacgcgccc aggttacgac cataatcgaa
     4801 agaacccaaa tctttatatt tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt
     4861 atcactctcc gctttatttc cggcaaactc cgcttcccac cgaccataac cagtcagttg
     4921 atcgttaatt tgagtttcac ctttaaaacc aaaacggata taactctggt cgccatcttt
     4981 actggcgtta tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt
     5041 accgtcttta ttatatattt ctgcagcctg tacagatgca gatgccacaa tgcccatcac
     5101 cactaatgcc agagtgctct ttttcatttt cattcctgat tttaattaac gcgcgaatat
     5161 tcagcgggag agccccgttg aaaacaggaa agtttttaac ctgagattgt taaagatata
     5221 ttacagatta atgatattct gaaaatgtgg taatttatta aatctgtaat aaaagcgtaa
     5281 acaactgccg ctacgcttgc tgatcccgcg caacaaaacg ccatgctttg ctcgcagatg
     5341 gttggcaacc gacgacagtc ctgctaaaac gttcgtttga tatcattttt cctaaaattg
     5401 aatggcagag aatcatgagt gacagccaga cgctggtggt aaaactcggc accagtgtgc
     5461 taacaggcgg atcgcgccgt ctgaaccgtg cccatatcgt tgaacttgtt cgccagtgcg
     5521 cgcagttaca tgccgccggg catcggattg ttattgtgac gtcgggcgcg atcgccgccg
     5581 gacgtgagca cctgggttac ccggaactgc cagcgactat cgcctcgaaa caactgctgg
     5641 cggcggtagg gcagagtcga ctgattcaac tgtgggaaca gctgttttcg atttatggca
     5701 ttcacgtcgg gcaaatgctg ctgacccgtg ctgatatgga agaccgtgaa cgcttcctga
     5761 acgcccgcga caccctgcgt gcgttgctcg atcacaatat cgttccggta atcaatgaga
     5821 acgatgctgt cgctacggca gagattaagg tcggcgataa cgataacctt tctgcgctgg
     5881 cggcgattct ggcgggtgcc gataaactgt tgctgctgac cgatcaaaaa ggtttgtata
     5941 ccgctgatcc gcgcagcaat ccgcaggcag aactgattaa agatgtttac ggcattgatg
     6001 acgcactgcg cgcgattgcc ggtgacagcg tttcaggcct cggaactggc ggcatgagta
     6061 ctaaattgca ggccgctgac gtggcttgcc gtgcgggtat cgacaccatt attgccgcgg
     6121 gcagcaagcc gggcgttatt ggtgatgtga tggaaggcat ttccgtcggt acgctgttcc
     6181 atgcccaggc gactccgctt gaaaaccgta aacgctggat tttcggtgcg ccgccggcgg
     6241 gtgaaatcac ggtagatgaa ggggcaactg ccgccattct ggaacgcggc agctccctgt
     6301 tgccgaaagg cattaaaagc gtgactggca atttctcgcg tggtgaagtc atccgcattt
     6361 gcaacctcga aggtcgcgat atcgcccacg gtgtcagtcg ttacaacagc gatgcattac
     6421 gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat actgggatat gaatacggcc
     6481 cggttgccgt tcaccgtgat gacatgatta cccgttaagg agctggccga tgctggaaca
     6541 aatgggcatt gccgcgaagc aagcctcgta taaattagcg caactctcca gccgcgaaaa
     6601 aaatcgcgtg ctggaaaaaa tcgccgatga actggaagca caaagcgaaa tcatcctcaa
     6661 cgctaacgcc caggatgttg ctgacgcgcg agccaatggc cttagcgaag cgatgcttga
     6721 ccgtctggca ctgacgcccg cacggctgaa aggcattgcc gacgatgtac gtcaggtgtg
     6781 caacctcgcc gatccggtgg ggcaggtaat cgatggcggc gtactggaca gcggcctgcg
     6841 tcttgagcgt cgtcgcgtac cgctgggggt tattggcgtg atttatgaag cgcgcccgaa
     6901 cgtgacggtt gatgtcgctt cgctgtgcct gaaaaccggt aatgcggtga tcctgcgtgg
     6961 tggtaaagaa acctgtcgca ctaacgcggc aacggtggcg gtgattcagg acgccctgaa
     7021 atcctgtggc ttaccggcgg gtgccgtgca ggcgattgat aatcctgacc gtgcgctggt
     7081 cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg atcccgcgtg gtggcgctgg
     7141 tttgcataaa ctgtgccgtg aacagtcgac aatcccggtg atcacaggtg gtataggcgt
     7201 atgccatatt tacgttgatg aaagcgcaga gatcgctgaa gcattaaaag tgatcgtcaa
     7261 cgcgaaaact cagcgtccga gcacatgtaa tacggttgaa acgttgctgg tgaataaaaa
     7321 catcgccgat agcttcctgc ccgcattaag caaacaaatg gcggaaagcg gcgtgacatt
     7381 acacgcagat gcagctgcgc tgacacagtt gcaggcaggc cctgcgaagg tggtggcggt
     7441 taaagccgaa gagtatgacg atgagtttct gtcattagat ttgaacgtca aaatcgtcag
     7501 cgatcttgac gatgccatcg cccatattcg tgaacacggc acacaacact ccgatgcgat
     7561 cctgacccgc gatatgcgca acgcccagcg ttttgttaac gaagtggatt cgtccgctgt
     7621 ttacgttaac gcctctacgc gttttaccga cggcggccag tttggtctgg gtgcggaagt
     7681 ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg gggctggaag cactgaccac
     7741 ttacaagtgg atcggcattg gtgattacac cattcgtgcg taaataaaac cgggtgatgc
     7801 aaaagtagcc atttgattca caaggccatt gacgcatcgc ccggttagtt ttaaccttgt
     7861 ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc gatatagctc agttggtaga
     7921 gcagcgcatt cgtaatgcga aggtcgtagg ttcgactcct attatcggca ccatttaaat
     7981 caataagtta cctcgcattt aagtaaacca cgttctcctc ttgtgccgta tttgtgccat
     8041 tgcgacttat aatcgcatcg attttgcttg cgtgctcggt gagatgcccg gctgaaaggt
     8101 gggcgtatct ttgaaccatt tcgagagttt cccatcctcc catctcttta agtgcaagaa
     8161 gagagacacc ggactgaacc agccagcttg cccaggtatg cctcagatca tggaagcgga
     8221 agttgctaat gcctgcccgc tttaacgctc ccttccatgc cttgttgctg tcggttctca
     8281 tcttccttac cgctgctgtt tttgttccgt cgcttcggta ggcaggtttg gtgtggacaa
     8341 atacccatct cttatggagc ccctgctgtt ttcttaatat ctggcatgcg gtttcgttaa
     8401 gaggaactcc gatcgcattg ccagcttttg tttcatcagg gtgcatccat gccattttct
     8461 tatccagatc gacctgtgac cactcaaggt ctgtaacgtt ggaacggcga aggcctgtcg
     8521 tgattgcaaa catgaccaca gggaagaagt gaggagcaat ttctgcaaac aggcgcttcg
     8581 attcctcctc tgtaagccat ctgatgcgtc cattcttaac gcgtggtgtt gatattttgg
     8641 gtgccctgtc aagccatccc cattcaacag ccatattgag aatggcgcga agtattgcca
     8701 gatgcctcgt cttcgttcct ttgcttgcaa gctttggttt atactccggc accggattgc
     8761 caagtcgcaa acacctgtcc cggctcatct cccagttcag gcgatggcgg cggttttcca
     8821 tcccgtctac cgcctccatt attttttctg ttgttatgtc agagagaatg gcttctctga
     8881 agtgcaacat ccagaacgat ataatgctct tgtcatcatc aatggacttc ttatccgatt
     8941 tctcacgcag ccaccgtatg caggcttcct tgaatagctt tttcggtgat tccccgagat
     9001 tttttactct ccacgct
//
LOCUS       DADMFL010000143         8988 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.144, whole genome shotgun sequence.
ACCESSION   DADMFL010000143 DADMFL010000000
VERSION     DADMFL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.144"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNZ27_003554"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNZ27_003554"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY8665738.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNZ27_003555"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNZ27_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY8665739.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="JNZ27_003556"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="JNZ27_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY8665740.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /locus_tag="JNZ27_003557"
                     /pseudo
     CDS             complement(5771..7252)
                     /locus_tag="JNZ27_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNZ27_003558"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNZ27_003558"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY8665741.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="JNZ27_003559"
                     /pseudo
     CDS             complement(7822..8133)
                     /locus_tag="JNZ27_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNZ27_003560"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNZ27_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY8665742.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8988)
                     /gene="csgA"
                     /locus_tag="JNZ27_003561"
     CDS             complement(8645..>8988)
                     /gene="csgA"
                     /locus_tag="JNZ27_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY8665743.1"
                     /translation="SGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVG
                     QGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVAQV
                     GFGNNATAHQY"
BASE COUNT         2249 a         2329 c         2132 g         2278 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccaga gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttct
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ctgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtcgct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctaccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctatgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaaa attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagcc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgcag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctat
//
LOCUS       DADMFL010000144         8985 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.145, whole genome shotgun sequence.
ACCESSION   DADMFL010000144 DADMFL010000000
VERSION     DADMFL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.145"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..182)
                     /locus_tag="JNZ27_003562"
     CDS             complement(<1..182)
                     /locus_tag="JNZ27_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAY8665744.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQV"
     gene            complement(316..1152)
                     /gene="bglG"
                     /locus_tag="JNZ27_003563"
     CDS             complement(316..1152)
                     /gene="bglG"
                     /locus_tag="JNZ27_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY8665745.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(1438..2163)
                     /gene="phoU"
                     /locus_tag="JNZ27_003564"
     CDS             complement(1438..2163)
                     /gene="phoU"
                     /locus_tag="JNZ27_003564"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY8665746.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
     gene            complement(2178..2951)
                     /gene="pstB"
                     /locus_tag="JNZ27_003565"
     CDS             complement(2178..2951)
                     /gene="pstB"
                     /locus_tag="JNZ27_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY8665747.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(3043..3933)
                     /gene="pstA"
                     /locus_tag="JNZ27_003566"
     CDS             complement(3043..3933)
                     /gene="pstA"
                     /locus_tag="JNZ27_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY8665748.1"
                     /translation="MAMVEMQTTAARAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3933..4892)
                     /gene="pstC"
                     /locus_tag="JNZ27_003567"
     CDS             complement(3933..4892)
                     /gene="pstC"
                     /locus_tag="JNZ27_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY8665749.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4979..6019)
                     /gene="pstS"
                     /locus_tag="JNZ27_003568"
     CDS             complement(4979..6019)
                     /gene="pstS"
                     /locus_tag="JNZ27_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY8665750.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(6267..7340)
                     /locus_tag="JNZ27_003569"
     CDS             complement(6267..7340)
                     /locus_tag="JNZ27_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8665751.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALVAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(7351..>8985)
                     /locus_tag="JNZ27_003570"
     CDS             complement(7351..>8985)
                     /locus_tag="JNZ27_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8665752.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
BASE COUNT         2319 a         2298 c         2020 g         2348 t
ORIGIN      
        1 acctgaaact gaccaccact ttccaccacc ataataatac cgggggtctt tttcagtacc
       61 tcagcttgcg ctttgctttc atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc
      121 agactcacaa tgttatctgc gcccccgact cctgcgacta tttttctggc taactccgtc
      181 ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc
      241 tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt agtgcggtaa caatccttta
      301 ctcagtaata atatttcagt gttctttgcg cacgcgctct atatttatgg ctaaaaacat
      361 aatctctgcg ggtgaaattt tacgttgata ctgcaaacca ataaaaatgg cgatccgttc
      421 cgcacattgc catgcttgcg ggtaattttg ttttactgct tgttgtaatg attcatcact
      481 atcgttaatt gaagcatgtt caagaatacg ccaggataaa aacttcagat gtgtaaccag
      541 tcgctgataa ctcaagcttt cttcctggta attaaggctg aactgaaatt ttattaattg
      601 cagcatttcg cgcattaact gcgtgacacc tgcaacatcc tccatatttc cgctcatttg
      661 ggcactgacc agatgcatgg caataaagcc cacttcatct ttcggtaact gcacgcccaa
      721 ccgtttatca ataatggtta acgcttcttc ccctagctgg aactctttcg ggtaaagccg
      781 ctggatatcc cacagcaacg ggttgggcag caacacgttt tgctgaaagc gtttaatcgc
      841 aaactggcaa tggtcagtta gcgagatata aatactgtcc tgtaattttc ccaagcgctc
      901 ctgcgctaaa gagataagac gatcacaggt tgccatcacc tcaagaggta tatgacttaa
      961 gagttcgctt aatcgcccgt tcagttcatg actgctcaag gcatactctt tttctattcc
     1021 acttgagtta attctttcgc cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac
     1081 tttttcccgc tgttgatcat caataaccac cacaacatta ttgttgagaa ttttggtgat
     1141 ttgcatgttc atagcaagga ccttttttat aaacaaaaaa acccgacttc accagtattc
     1201 tctggttatg tcaggttttg cctgcgaatg cagtaacaat ccagtcattt attaatggtt
     1261 tttataacga acatccaggt tcggaaatta atttaattgc gtgcagagaa aatagcaatg
     1321 cgctattgat aaaaatatga ccatgctcgc agttattaac tttgtgtaat tttaggaatt
     1381 tataaagtta tatataacaa atcccaataa ttaagttatt gggatttgtc tggtgaatta
     1441 tttgtcgcta tcttttcccg ccagcagttt atccagctca tcactgccta catgacggaa
     1501 atcctgaccc ttcacgtagt agaagataaa ctcacaaata ttctggcagc ggtcgccgat
     1561 acgttcgatt gaacgcgcac agaacagcgc ggtaagtacg ctcggaatag tgcgcgaatc
     1621 ttccatcatg taggtcatca gttgacgaac aataccttcg tattcctgat cgactttttt
     1681 atcttcacga taaatacgta ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac
     1741 gtcgtgcagc atctggatgg tatgacggcc cagcgactcc agacttacca gcaacggctg
     1801 atgctgctgg gagaatttct ccagcgcagt acggcagatt ttgtccgcca cgtcgccaat
     1861 acgctccagc tcggcaatgg ttttactgat caccataacc agtcgcaggt cgctcgccgt
     1921 cggctgacgt ttggcgataa tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt
     1981 gacgttcttg tcgccttcga tcacgcgctt cgccagatcg ctgtcctggt tatgcatcgc
     2041 ggtgattgca tcagaaagct gctgctccac catgccgccc atggtcatca cctgcgtgcg
     2101 gatactttcc agttcggcgt tgaactggcc ggaaatatgt ttattaagat tgagactgtc
     2161 cataacgcac tcctgaatca accgtaacga ccggtgatgt agtcttctgt ttgtttcttc
     2221 gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa tcaattcgcc caggtacata
     2281 aatgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg
     2341 gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac gcccggtaga gatggggtcg
     2401 agcgccgaac acggttcgtc gagcagcagc acttccgggc gaatggcgat accacgcgca
     2461 atacacagac gctgttgctg accaccagag agagagtaac cgctctggtg caatttatct
     2521 ttggtttcgt tccacaatgc ggctttggtc aatgcccact gcacgcgctc gtccatgtcg
     2581 gcacgggaga gcttctcaaa cagacgaacg ccaaaggcga tgttgtcgta gatggacatc
     2641 gggaacggcg tcggtttctg gaacaccatg cccactttcg cacgcagcag ggcgatatcc
     2701 tgagagttgg tcaggatgtt gtcgccatca agcagaattt caccttccgc acgctgctcc
     2761 gggtacagtt caaacatttt gttgaaggta cgcagcagcg tcgatttacc gcagccggac
     2821 ggcccgataa acgccgtaac ctggtttttg gcgatatcca ggttgatgtt tttcagggca
     2881 tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa ttttactcgg ggcagtttca
     2941 accatactca ttgcaatctt ttcctcatcg tacctgatgc ttttcgctaa tcaggcttac
     3001 cgtagggccg ggtaaggcgt tcacgccgca tccggcaaag tgttaaccgt gtttattctt
     3061 cgcaaaaaca acgcgcgcca gaatgttcag cagcaggacg cacagggtaa tgatcaatac
     3121 cccggcccag gccagttgtt gccattccgc aaacgggctc atcgcaaact taaagatcgt
     3181 caccggcagg ttggcgatcg gctgcatcat gtccgtgctc cagaactggt tggagagcgc
     3241 ggtaaacagc agcggcgcgg tttcaccggc aatacgggca atcgccagca ggataccggt
     3301 cataatcccg gacaccgacg ctttcagcgt aatcgcggag atcatcttcc acttcggtgt
     3361 acccagcgca taagccgctt cacgcaggct gtccggcacc agtttcagca tgttctcggt
     3421 ggtgcggata acgatcggca cctgcaacag cgccagcgca attacgcccg cccagccgga
     3481 gaagtgctcc atctgtgcca ccacgatggt gtagacaaac agaccaacca caatcgacgg
     3541 cgcggagagc agaatgtcgt taatgaagcg aatcacttct gccagccagg atttacgacc
     3601 atattccgcc agataaatcc ccgccataat gcccagcggc gtaccgaata ccgtggccca
     3661 caaaattaac agcccgctac ccgccagggc gttcgccaga ccaccacctt ccgtattggg
     3721 cggcggcgtc atttcagtga acagcgccag cgacataccg tcgataccgc gagtgatggt
     3781 ggacattaaa atccagatca gccagaacag gccgaaggcc atcgtcgcca tcgagagcgt
     3841 cagcgcaata cggtttttga ggcggcgacg cgcctgcatt ttgcggcgag attcagcccg
     3901 cgccgcagtg gtttgcattt caaccatagc cattagcgtg ccccctcatt cttagccagg
     3961 cgcataatca taaacttcga tgcggcgagg acgatgaagg taatcacaaa caggatcagg
     4021 cccagttcca tcagtgcggc aacgtgcaga ccggattccg cttccgcaaa ttcgttcgcc
     4081 agcgcagagg tgatactatt gcccggcatg tacagcgagg cgctgtcgag ctggtaggtg
     4141 ttaccgatga taaaggtcac cgccatggtt tcaccgagcg cgcggcccag ccccagcatg
     4201 atgccgccga taacaccatt tttggtgaac ggaagaacga tacgccagat aacttcccag
     4261 gtggtgcagc caataccgta ggccgactct ttcatcatca ccggggtttg ttcgaacaca
     4321 tcacgcatta ccgccgcaat gtacggaata atcatgatgg cgaggatcac gcctgccgcg
     4381 aggataccga taccaaatgc ggggccagag aacagcgcgc caacaatcgg gatattcgac
     4441 atgatattgc cgaccggctc ctgaaagtaa acggcgaaca gcggcgcaaa gataaacagg
     4501 ccccacatgc cgtaaacgat acttggaatg gctgccagca gctcaatggc gatacccagc
     4561 gggcgtttca gccagccagg cgcaagctca gtcaggaaca gggcgatacc gaaactcacc
     4621 gggacggcga tcagcagcgc gataaacgaa gtcaccaacg taccgtagat cggcaccagc
     4681 gccccgtaga tatcgttcgg tgcatcccac tctttggtcc ataggaaagc cagaccaaat
     4741 ttctgaatgc tcggccagga ggagatgatc agagagacaa taatgccacc caacatcaat
     4801 agcacaatca gcgccgccag ttttaccagc acgctgaaaa ttatgtcacc ctttttaccc
     4861 ggtgggttaa aagcgggctt ggttgcagcc ataagttact cttcagttaa acgcgtttac
     4921 aaagtcattt tgtaatgccg gatgcggcgt aaaacaccgt acccgacctg gagttttatt
     4981 agtacagcgg cttaccgcta ctgtctttaa tattggtctt ccacgcagcg cgaacctgtt
     5041 caactacact atccggcagg ctagcgtaat ccaggtcgtt cgcctgttta gccccggttt
     5101 tgtacgccca gtcgaagaat ttcagcacct ctgtgccttg ttctggtttc ttctgatctt
     5161 tgtggatcag aatgaacgtg gtagaggtaa taggccatgc atcttcgcct ttctggttgg
     5221 tcagatcctg agcgaaggtt ttgctccagt ctgcaccttt tgctgcatta gcgaagtttt
     5281 cttcggtcgg actaaccggt ttaccatcag cggagatcag tttggtgtac gccaggttgt
     5341 tctgcttcgc gtaagcatat tcaacataac caattgcacc cggcagacgc tgaacgaacg
     5401 cggcgatacc gtcgttacct ttaccgccca gaccgatcgg ccattttacg gtagagccag
     5461 taccaacgtt gtttttccac tcttcgttca ctttcgccag gtagctggtg aagacgaagg
     5521 aagtcccgga gccatctgcg cggcgtacta cagcaatgtt ttgtgaaggc agtttcagac
     5581 ccggattcag tttggcgatg gcttcatcat cccacttctt gattttgccc aggtagatgt
     5641 cgccgagggt tttaccatcc agcaccagtt cgccagactt cagccctgga atgttaaccg
     5701 ccagcaccac gccgccaatc acggtcggga actggaacag accttcctga gccagttttt
     5761 cgtcagacag cggcgcgtca gaggcaccaa aatcaacggt attagcgata atctgtttta
     5821 cgccaccgga agaaccgata ccctggtagt taactttatt accggtttct ttctggtaag
     5881 tgtcagccca tttggcatac accggcgcag ggaaggttgc acctgcacct gtcaggcttg
     5941 cttctgcaaa cacagagaaa gcactcatcg ataaggtcgc ggcgacaaca gttgcgacgg
     6001 tggtacgcat aactttcata atgtctcctg ggaggattca taaagcattg tttgttggct
     6061 acgagaagca aaataggaca aacaggtgac agttatatat aaggaatatg acatttttat
     6121 gacagaaagg aattaccaaa taacttactg attttaaatg tgaagatgaa aatcccacat
     6181 gccatctttt tgtcatgatg catttaaaag tgataaagaa atgggtgaga tgacaacgat
     6241 gtgatgaaca atcgttgtca gacctattag cgcacaatct caacagttat cgtcgctgtt
     6301 gcagtaaatt gccccggggc tgggcgagct ccagtaaggc tcgcaggagc agctgaaaaa
     6361 gttacactac ctgatttttc accatacact gttgatttat ccatgtctgt tggcaactca
     6421 ccaccgttca ctttgatttc cttattattt ttgtcgtaaa tttttatccc cacgttagag
     6481 tttgaagtta cgaccattga attgtccgta ttgggttcgg tagcataaaa agacgtactg
     6541 atatcatcgg tattcagtat ccctaggttt tcacactcaa tagtgactgt tttagtaatt
     6601 ttatgtgttg ttaccgcact tccaacaaca gatgaaaaat ctgcagctgg tatttcttta
     6661 aaatcaaaac aaactggctt taaattattt atttcgcaac tttgcggagc tgtaatatcg
     6721 ccccgaatat aaacatcagc catcggctgt gagtaatcca ccggggtgtc acggctaatt
     6781 gttccataca atgtcactat ctttgtttca ggaataatca attttcctgc aaatgtcttc
     6841 ttgatataaa aatatactat cgctttagcc ccactggata tacttaaagg ttcaatgcgg
     6901 ttgcaattgt actttgtatt accagacggg ttgttagcat gatattcaaa aggtgcattc
     6961 acatacccaa ccccaagaat atctatagca atcccaacat caaggttcgg attaagatca
     7021 taataattaa tattagagtg aaccccagca taagttaatg cctggtttat ttttgacgta
     7081 taatagatat aggggaattc tcctacgtta ccagtatcac aatgacagcg tccaagatat
     7141 gaggatccat tagttacagt ggtgttaaat gtcgttccaa ccttattttc gttcgcggta
     7201 ataagatatc ccgagaaatc aagcgtactg tgaaatacgc cattatcagg ggtacagata
     7261 cctttaggag ctacaagtgc tacactactt aacgagagca taaacacaga aaagcaccac
     7321 tgtttgatat acttgttcat aaaaatcctt ttaatgacat gtcacaccct gttcaattaa
     7381 tccgctctca ctttctggtg tcgaaagtga ataattgaag atgcattttg ttgaggcgtt
     7441 ttctccccag gataacgtaa cctgtccttt ttctggtaga ccagaaagat aaagttcgcc
     7501 acggtcaccg acaattcctg aatctgccaa acttgcatct tgtatcgcag caatggctcc
     7561 aaaaggtacc ggtttaccat ttgcatttaa cactctgaag agaacgcgat aacccacatg
     7621 agtcacaaac tccgctttaa ccactgcacc acgcgtggga accatcgtaa cgacggtatt
     7681 atcaagttct atattcgact tcgagaaata gttatcgctg attgctaccc gattttcatc
     7741 ataagggttt agctgtgtct tgaccgtata gccgcgccag tccgtcgcag caccgcgcat
     7801 attatctatt tctaacccgg cggccccagg tgctttaaca agagcaatag tttcacctaa
     7861 ctcctgactt aacgttaccc cttcgctgtg tgccagaatt ccccctctga taccataatt
     7921 cagcctctgg gaattttggc tgtagttata gctgccgttc accgtgccat aagagccatc
     7981 ataggtcagg gacatattac cgccaacctc atcatcatga ctgttatagg actgcattaa
     8041 attccatgaa aggttatttc tttccagcgc cgtaccgctg agtccgagtt gatgtgagct
     8101 accggaatta tcgttttgcg aaacagaata ggtggcgtaa ctattacccc accagcccga
     8161 taaaggaaca gataaagaga gcatgtagat ctgctcactg gtgttatcac tggcatggtt
     8221 gttccccgtc ccctgtctgg cagtattgga ataactccag gagacgttcc acgagagacg
     8281 cttccagcta ctgctgtagc caaattgcat taatcgttcg acatcatcgg tatgccagta
     8341 ttgttgccgg cttaatgtca ggtataaatt gccataatct ctccccaacg actgcgttag
     8401 cgttccctcc acgcgactac gtcggttacc tgtttcccaa aagtcttcag ggctgttgcg
     8461 ccgcgatgcc cactcattga gggtatagta gccctcggtg gagtaacgat agccaaccag
     8521 gcgcaagcta gtgtctgtgt cgtcaaatag ttttgaatag agaaagcgat atgactgacc
     8581 cgtttctgta tcatcatggc taaaattcgc acgagcatgt gtcacatcga aagaaattgc
     8641 gcccaaagcg ccaagatctt taccgacacc cagagctgca gaaccataat tttcagcagc
     8701 ctgcatacct ccatataaag tgatcccgtg ggaaaaacca tgaagtattt gcgcctgaag
     8761 gacgtccggt gtaaatccgt cttcatcgcg caattcaccc acgctgacat caacatctgt
     8821 cagaccttca cgtttaagaa tcgccaatga agcataaggt tgggtgaatc gttgttcgct
     8881 accgtcttct tctttgatgg ttacgtccaa atcgccacct gtacttgcgg tgttgagatc
     8941 ggtgatctca aaagcacctt gtggaacggc tgactgataa atcac
//
LOCUS       DADMFL010000145         8895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.146, whole genome shotgun sequence.
ACCESSION   DADMFL010000145 DADMFL010000000
VERSION     DADMFL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.146"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            369..2887
                     /locus_tag="JNZ27_003571"
                     /pseudo
     CDS             369..2887
                     /locus_tag="JNZ27_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JNZ27_003572"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JNZ27_003572"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY8665753.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="JNZ27_003573"
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="JNZ27_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8665754.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGSDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVADIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLSGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5343..5564)
                     /locus_tag="JNZ27_003574"
     CDS             complement(5343..5564)
                     /locus_tag="JNZ27_003574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8665755.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(5760..7283)
                     /locus_tag="JNZ27_003575"
     CDS             complement(5760..7283)
                     /locus_tag="JNZ27_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY8665756.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQK"
     gene            complement(7280..8041)
                     /locus_tag="JNZ27_003576"
     CDS             complement(7280..8041)
                     /locus_tag="JNZ27_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY8665757.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="JNZ27_003577"
     CDS             complement(8038..8697)
                     /locus_tag="JNZ27_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8665758.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2169 a         2358 c         2325 g         2043 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc gggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggatg
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag caattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc agcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggcg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt gtcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtggaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgagcggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacaggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagcgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcgccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtacagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcggttggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacggtact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtagttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agagg
//
LOCUS       DADMFL010000146         8862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.147, whole genome shotgun sequence.
ACCESSION   DADMFL010000146 DADMFL010000000
VERSION     DADMFL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.147"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(296..1156)
                     /locus_tag="JNZ27_003578"
     CDS             complement(296..1156)
                     /locus_tag="JNZ27_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY8665759.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAGWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            1242..2777
                     /gene="lsrA"
                     /locus_tag="JNZ27_003579"
     CDS             1242..2777
                     /gene="lsrA"
                     /locus_tag="JNZ27_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY8665760.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            2771..3799
                     /gene="lsrC"
                     /locus_tag="JNZ27_003580"
     CDS             2771..3799
                     /gene="lsrC"
                     /locus_tag="JNZ27_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY8665761.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            3799..4816
                     /gene="lsrD"
                     /locus_tag="JNZ27_003581"
                     /pseudo
     CDS             3799..4816
                     /gene="lsrD"
                     /locus_tag="JNZ27_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
     gene            4828..5851
                     /gene="lsrB"
                     /locus_tag="JNZ27_003582"
                     /pseudo
     CDS             4828..5851
                     /gene="lsrB"
                     /locus_tag="JNZ27_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            5878..6753
                     /gene="lsrF"
                     /locus_tag="JNZ27_003583"
     CDS             5878..6753
                     /gene="lsrF"
                     /locus_tag="JNZ27_003583"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY8665762.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            6777..7067
                     /gene="lsrG"
                     /locus_tag="JNZ27_003584"
     CDS             6777..7067
                     /gene="lsrG"
                     /locus_tag="JNZ27_003584"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY8665763.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            7106..7864
                     /gene="tam"
                     /locus_tag="JNZ27_003585"
     CDS             7106..7864
                     /gene="tam"
                     /locus_tag="JNZ27_003585"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY8665764.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(7868..8782)
                     /locus_tag="JNZ27_003586"
                     /pseudo
     CDS             complement(7868..8782)
                     /locus_tag="JNZ27_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2123 a         2158 c         2393 g         2188 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc caccccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg ttgtccacct aaggttatga acaaattaaa agtagaaata
     1201 catttgttca aaactcacct gcaaaactga acgggggaaa tatgcaaacg agtgataccc
     1261 gcgcgttacc gctactttgc gcccgctcgg tttataaaca gtattcaggg gtcaatgtcc
     1321 tgaaaggcat cgattttacg ttgcatcagg gggaggtcca cgccctgctc ggcggcaatg
     1381 gtgccggtaa atcgacgtta atgaagatta ttgccggtat tacccctgct gatagcggta
     1441 cgctggagat tgggggcaac aactacgcca gattaacgcc agttcatgct catcagctgg
     1501 gcatttatct cgttccccag gaaccgctgc ttttcccaag cctgtcgata aaagaaaaca
     1561 tcctgtttgg gctggcaaaa aaacagctct ccatgcagaa aatgaagaac ttgctggcgg
     1621 cgctgggctg ccagtttgat ctgcatagtc tggcaggatc gctggatgtc gccgatcgcc
     1681 aaatggtgga aatccttcgc gggctgatgc gcgactcgcg gattctgatc ctcgatgaac
     1741 ctaccgcctc gcttacccct gcgggaaccg aacgcttgtt tagtcgcttg caagagctgc
     1801 ttgctactgg cgtgggtatt gtttttatct cgcataagct gccggaaatt cgccagattg
     1861 ccgatcgaat tagcgtgatg cgcgacggaa ccatcgcctt aagcggcaaa accagcgaac
     1921 tgtctaccga cgacattatt caggccatca ccccagcggt acgggaaaaa tcgctctcag
     1981 ccagccaaaa attatggctg gagttacctg gtaaccgccc acaacatgcc gtcggaacgc
     2041 cggtgctgac actggaaaat ctgactggcg aaggcttcag gaatgtcagc ctgacgctca
     2101 atgccggaga aattctgggc ctggctgggc tggtgggagc cggacgcaca gaactggccg
     2161 agacgctcta tggtctgcgt actttgcgtg gcggacgcat tatgctgaat ggtaaagaga
     2221 tcaataaatt atccactgga gaacgtttac tgcgcggtct ggtttatctg ccggaagatc
     2281 gccagtcatc cggactgaat ctcgatgctt cgctggcctg gaacgtctgc gcccttactc
     2341 ataaccttcg tggattctgg gcgaaaaccg cggaagataa tgccaccctg gaacgttatc
     2401 gtcgggcgct gaatattaaa tttaaccaac cggaacaagc tgcacggaca ttatccggta
     2461 gcaaccagca aaaaatcctc attgccaaat gcctagaagc ttcgccgcaa gcattgattg
     2521 tcgatgagcc gacgcgcggc gtggatgtct cggcccgtaa tgatatctac cagctgttgc
     2581 gcagcatcgc cgcacagaat gtggctgtgc tgcttatctc ctctgatatg gaagagatcg
     2641 aactgatggc agatcgtgtg tatgtgatgc atcagggcga aattacccac tctgcactga
     2701 ccgggcgcga tattaatgtc gagactatta tgcgcgttgc cttcggcgat agtcagcgtc
     2761 aggaggcatc atgctgaagt ttattcagaa caaccgtgaa atcacggcat tgctggcggt
     2821 ggtgctgctg tttgtattac ccggttttct cgaccgccag tatttaagtg tgcaaacgct
     2881 gaccatggtt tatagcagcg cgcaaatcct gatcctgctg gcaatgggcg cgacgctggt
     2941 aatgcttacg cgcaatattg atgtttcagt gggttcgatt accggaatgt gcgcggtgct
     3001 gttggggatg ttactgaacg caggatattc actacctgtt gcctgtgtcg cgactttact
     3061 gcttggtttg ctcgcgggat ttttcaacgg tgccctggtc gcgtggctaa agatccctgc
     3121 cattgttgcc acccttggca cgttagggtt gtacagaggc atcatgttgc tgtggactgg
     3181 cggtaaatgg attgaagggt tacccgccga actgaaacag ctctccgccc cgctgctgct
     3241 tggcatttca gcaattggtt ggttgacgat aattctggtg gcatttatgg cctggctgct
     3301 ggcgaagacg gcgtttggac gcagttttta tgccacgggc gataatttac agggcgctcg
     3361 tcaactgggc gttcgtacgg aagccattcg cattgtggca ttttcgttga acggctgcat
     3421 ggcggcactg gcgggaattg tgtttgcttc gcagattggt tttatcccca accagaccgg
     3481 taccgggctg gagatgaaag caattgcagc ctgcgtgctg ggcggcatta gtttgctcgg
     3541 tggttccggt gcgatcatcg gtgcggtact cggcgcatgg ttcctgacgc agatcgatag
     3601 cgtactggtg ctgttgcgca ttccggcatg gtggaatgat tttatcgcgg gtctggttct
     3661 gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg ctggaacgta atctacggcg
     3721 gcaaaaatat gcccgcttta tgacgccacc gccatccgtt aaacccgctt cgtcaggtaa
     3781 aaaacgggag gccgcataat gcgtattcgc tacggttggg aactggctct tgccgcactg
     3841 ctcgttattg agattgtcgc atttggtgca attaacccgc gaatgttaga tctcaatatg
     3901 ttgctgttca gcaccagtga ctttatctgc attggcattg tcgctctaca tctgcattgg
     3961 cattgtcgct ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg
     4021 ttcgaccatc ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat
     4081 gccgctggcg atactcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg
     4141 attaattatc tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt
     4201 tgccggaagc gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg
     4261 tggattcccg atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc
     4321 gctgattatc ttcctgatat gtctcctcgt tttctggttc tggctgcata aaacccatgc
     4381 cggacgtaat gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc
     4441 agttaaccgc accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc
     4501 tgtgctgctg gtatcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat
     4561 gcccgccatc accgccgtgg tgcttggcgg ggccaatatt tatggtggtt ccggttccat
     4621 tatcggcacc gccattgcgg ttttattagt gggatatttg cagcaaggtt tgcaaatggc
     4681 aggagtgcca aatcaggtgt ccagcgccct ttccggtgcg ctgcttatcg tcgttgtcgt
     4741 aggtcgttcc gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa
     4801 taacccattg ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta
     4861 cttagcgctc ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca
     4921 tttattccca aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa
     4981 gcgggtaaag agctgagcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct
     5041 ggtcaggtac agttgattaa taacttcgtc aatcaaggtt ataacgccat tattcgtttc
     5101 tgcggtttcg cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag
     5161 agtgctgacc tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg
     5221 aacgcccgcc cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga
     5281 caaagccaaa gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt
     5341 gaaagaagcg aaagcgaaaa tcgccaaaga gcatccaggc tgggaaattg tcactacgca
     5401 gtttggctat aacgatgcca ctaaatcgtt acaaaccgca gaaggaatat taaaagcgta
     5461 tagcgatctc gacgccatta tcgcccccga tgccaacgcc ctgcccgctg ccgcacaagc
     5521 cgcagaaaac ctgaaaaatg acaaagtagc gattgtcgga ttcagtacgc caaatgtgat
     5581 gcgcccgtat gtagagcgcg gcacggtgaa agaatttgga ctgtgggatg tggttcagca
     5641 aggcaaaatt tcagtgtatg tcgcggatgc attattgaaa aaaggatcaa tgaaaacggg
     5701 cgacaagctg gatatccagg gcgtaggtca ggttgaagtc tcgccaaaca gcgttcaggg
     5761 ctatgactac gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa
     5821 caaagagaat atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg
     5881 gcagatttag acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat
     5941 atccctttta ccctgaaagg ttgcggtgcg ctggattggg gaatgcagtc acgcttatcg
     6001 tggatattta atccgaaaac gggtaaaact gtgatgctgg cctttgacca tggttatttt
     6061 caggggccga ctaccggact tgaacgcatt gatataaata tcgccccgct gtttgaacat
     6121 gccgatgtat taatgtgtac gcgcggcatt ttgcgcagcg tagttccccc ggcgaccaat
     6181 aagccggtgg tactgcgggc gtcaggtgcg aactctattc tggcggaatt aagtaatgaa
     6241 gccgtggcgt tatcgatgga tgatgccgtg cgcctgaaca gttgcgcggt ggcggcgcag
     6301 gtttatatcg gcagcgaata tgaacatcag tcgatcaaaa atattattca gctggtcgat
     6361 gccggaatga aagtgggaat gccgaccatg gccgtgactg gcgtgggcaa agatatggtg
     6421 cgcgatcagc gttatttctc gctcgcgact cgaatcgccg ctgaaatggg ggcgcaaatt
     6481 atcaaaacct attatgtcga aaaaggtttt gaacggattg ttgccggatg tccggtaccc
     6541 attgttattg ctggcggtaa aaaattaccg gagcgcgagg cgctggaaat gtgctggcag
     6601 gctatcgatc agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat
     6661 ccggtggcga tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac ggctgatcgg
     6721 gcatatgaac tctatctgag tgaaaaacag taactgcgga tttaaggaga agaattatgc
     6781 acgtcacact ggttgaaatt aacgttcatg aagacaaggt tgacgagttt atcgaagttt
     6841 ttcgccagaa ccacctgggc tctgtacagg aagaaggtaa tttgcgcttc gatgtcttac
     6901 aggacccgga agtgaattcg cgcttttata tttacgaagc ctataaagat gaagacacag
     6961 tggcattcca taaaaccacg cctcactaca aaacctgtgt cgcgaaactg gaatctttaa
     7021 tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc tacaattggg
     7081 gtaacgcgct gacgggagta aaaaaatgtc tgactggaac ccctctttat atctgcactt
     7141 ttccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc agagtgccgc tggagaatgt
     7201 cgaatatgtt gccgatcttg gctgtggccc aggtaacagc accgccctgc tgcatcaacg
     7261 ttggcctgcg gccaggataa caggcatcga ctcgtctccg gcgatgattg ctgaagcgcg
     7321 cagtgctttg ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca
     7381 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
     7441 gtttcctcac ttggtttctt tacttaatcc gcagggcgtg ctggcagtac agatgccaga
     7501 taactggctg gagccgaccc atgtgctcat gcgcgaagtt gcttgggaac aaaactaccc
     7561 ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac tacgatattt tgagcgaagc
     7621 cggatgtgag gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccaggc
     7681 gattatcgat tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
     7741 cgaacagcag ctttttctta cgcgctacca tcagatgctg gaagagcagt atccactgca
     7801 agagaacgga cagatactgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga
     7861 gtaattatca cgtcagctgg taatgacgat cgggaagaat ttttgctgga atttctgctt
     7921 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
     7981 cattgttttc agtaccgaac ggatcttcca gttcttccgc cagacaatcc agcgaaataa
     8041 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
     8101 cgaacggcag catgatacaa aacagataaa cggtacgatg caaaatcagg gtgtaggcaa
     8161 agggaattgg cgtgtaggca atgcgctcac agcctgccag aaccgctgaa atatcattaa
     8221 gccgatcgtt gaggctaata aacaggatat ctgaaagctg tccattgcgg cgctgaaccg
     8281 ccaaccattc tcccattatt aacaagatac ggttagctgg agagttcgaa gccagtacac
     8341 gctgaagatc tccagtcttg agataatgag ccagcacttc cgcctgtggc tatttgcgta
     8401 atgtcatgcg taaacagtgg gcgaaggcga tttgcagcct ggcaaactcc cttacacttg
     8461 ccgaatccgg caatgtcgtt tttacctctc gcagtaacga ccgtgaggca atcatcaact
     8521 gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc ggcattatta cgaaaaccaa
     8581 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttgatgccca
     8641 gatgcgtgta ccagggcagc gtgaatataa cagcaataga aaagagaaaa ttgagtagta
     8701 agcgcaagga tatcttggat aatactgagc cgtgccagac aaaaatacgg cgcagccagt
     8761 gttgttgtgg acgaacaatc atggttatct tcaggcgtgg aaaagtcgcc ctattaaacg
     8821 tgattacgat cacattctca agacgctact tacaaattac ct
//
LOCUS       DADMFL010000147         8775 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.150, whole genome shotgun sequence.
ACCESSION   DADMFL010000147 DADMFL010000000
VERSION     DADMFL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.150"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(16..756)
                     /gene="cysQ"
                     /locus_tag="JNZ27_003587"
     CDS             complement(16..756)
                     /gene="cysQ"
                     /locus_tag="JNZ27_003587"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY8665765.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            946..2889
                     /gene="cpdB"
                     /locus_tag="JNZ27_003588"
     CDS             946..2889
                     /gene="cpdB"
                     /locus_tag="JNZ27_003588"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY8665766.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTMNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINVNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3015..3395)
                     /locus_tag="JNZ27_003589"
     CDS             complement(3015..3395)
                     /locus_tag="JNZ27_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8665767.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3484..4344
                     /gene="qorB"
                     /locus_tag="JNZ27_003590"
     CDS             3484..4344
                     /gene="qorB"
                     /locus_tag="JNZ27_003590"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8665768.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            4453..5418
                     /locus_tag="JNZ27_003591"
     CDS             4453..5418
                     /locus_tag="JNZ27_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8665769.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5526..6188
                     /gene="ytfE"
                     /locus_tag="JNZ27_003592"
     CDS             5526..6188
                     /gene="ytfE"
                     /locus_tag="JNZ27_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY8665770.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6233..7645)
                     /gene="cycA"
                     /locus_tag="JNZ27_003593"
                     /gene_synonym="dagA"
     CDS             complement(6233..7645)
                     /gene="cycA"
                     /locus_tag="JNZ27_003593"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY8665771.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7953..8573)
                     /locus_tag="JNZ27_003594"
     CDS             complement(7953..8573)
                     /locus_tag="JNZ27_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8665772.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2222 a         2304 c         2225 g         2024 t
ORIGIN      
        1 ttctgccatc tgaatttagt aaatggacac tctgaacccc ggattcagga acgactcgcg
       61 cggagtgtaa tccagcggtt taccctgcca gtcgtgaacg tgcgctccgg cagctgcagc
      121 cacagcatgt ccagcggcgg tgtcccaaat attcgttggt ccgaagcgcg ggtacagctg
      181 cgcctgtcct tccgccacca ggcagaattt cagcgaagag ccgatggacg tggtctgatg
      241 ttcgccaagc tgttgcagat actctttcag ctccgcatct gcatgggaac ggctgataac
      301 caccagcggc gggcgcgcat cgcggacctg aatcagcttg cgcacaccgc actcttcttt
      361 ccaggccttg ccttctgccg cgctgtacat tacgttcatt accggcgcat acaccacacc
      421 taaaatcggt ttgccatggt caatgagcgc aatgttaacg gtgaattcgc cattacgttt
      481 aataaactct ttagtaccat ccagcgggtc taccagccag taacgctgcc agtgctgacg
      541 gacttcccaa ccgggaggat cttcttcaga aaggaccgga atatccggtg ccagcgtacg
      601 taaaccgtcc atgataacgg tgtgagcggc aatatccgct gccgttaccg gagaattgtc
      661 cgctttgctg acgacgtcca tcggtttcgt cccgtcgtag acctgcataa tggcatcgcc
      721 tgcattccgt gcaagctggc atacttgatc taacatttct ccacctcgtc tctgtgagcg
      781 gtgttaactt attgttttac ttatacccta tcgttaatga atgcgccaac tgtgatagtg
      841 tcatcatttt caaagcgtaa aattgtggca ttcttcactg ttctataagt aaggcgttta
      901 ttctcccttt tctttcgtat tcccgatgat aaaaggatgt ccctgatgat taagtttagc
      961 gcaacgctcc tggccacgct gattgccgcc agtgtgaatg cagcgacggt cgatctgcgt
     1021 atcatggaaa ccactgatct gcatagcaac atgatggatt tcgattatta caaagacacc
     1081 gccacggaaa aattcggact ggtacgtacg gcaagcctga ttaacgatgc ccgcaatgaa
     1141 gtgaaaaaca gcgtactggt cgataacggc gatttgattc aggggagtcc gctggccgat
     1201 tacatgtcgg cgaaaggatt aaaagcaggt gatgttcatc cggtttataa ggcgctgaat
     1261 acgctggatt atacggtcgg tacactcggc aatcatgaat ttaactacgg tctggattac
     1321 ctgaaaaatg cgctggcggg agcgaaattc ccttatgtaa atgccaacgt cattgacgcc
     1381 agaaccaaac agccaatgtt tacaccgtat ttaattaaag ataccgaagt ggtcgataaa
     1441 gacggaaaaa aacagacgct gaagattggc tatattggcg tcgtgccgcc gcaaatcatg
     1501 ggctgggata aagctaattt atccggaaaa gtgacgatga atgatattac cgaaaccgtg
     1561 cgcaaatacg tgcctgaaat gcgcgagaaa ggtgccgatg tcattgtcgt tctggcgcat
     1621 tccgggctgt ctgccgatcc gtataaagta atggcggaaa actcagttta ttacctcagt
     1681 gaaattccgg gcgttaacgc cattatgttt ggccatgctc acgccgtttt cccgggtaaa
     1741 gattttgctg atatcgaagg ggctgatatc accaaaggca cgctgaatgg tgttccggcg
     1801 gtaatgccgg gcatgtgggg cgatcatctt ggggtggtcg acttacaact cagtaatgac
     1861 agcggtaaat ggcaggtgac gcaggcgaaa gcggaagcac ggccgattta cgacatcgcc
     1921 aataaaaaat ccctcgcggc ggaagacagc aagctggtag aaacactcaa agccgatcac
     1981 gatgccacac gccagttcgt cagcaagcca atcggtaaat ctgccgacaa tatgtatagc
     2041 tatctggcgc tggtgcagga cgatccgacc gtgcaagtcg tgaacaacgc gcaaaaagcg
     2101 tatgtcgaac attacattca gggcgatccg gatctggcaa aactgccggt gctttcagct
     2161 gccgcaccgt ttaaagttgg tggtcgcaaa aatgacccgg caagctatgt ggaggtggaa
     2221 aaaggccagt tgaccttccg taatgccgcc gatctttatc tctaccccaa tacgctgatt
     2281 gtagtgaaag ccagcggtaa agaggtgaaa gagtggctgg agtgctccgc cggacagttt
     2341 aaccagattg ctcctaacag cacgaaacca cagtcactca tcaactggga tggtttccgc
     2401 acttataact ttgatgttat tgatggtgtg aattatcaga ttgatgttac ccaacccgcc
     2461 cgctatgacg gcgagtgcca gatgattaat gtcaatgcag agcggattaa gaacctgacc
     2521 tttaatggca agccgattga tccgaacgct atgttcctgg ttgccaccaa taactatcgc
     2581 gcttacggcg gcaaatttgc cggtacgggc gacagccata tcgcttttgc ttcaccggat
     2641 gagaaccgct cggtgctggc agcgtggatt gctgatgagt cgaaacgtgc gggggaaatt
     2701 catccggcgg cagataacaa ctggcgttta gcaccgatag ctgccgataa gaaactggat
     2761 atccgtttcg aaacttcccc gtcagataaa gccgcagcgt ttattaaaga gaaagggcag
     2821 tatccgatga ataaagtcgc gaccgatgat atcgggtttg cgatttatca ggtggatttg
     2881 agtaagtaaa acacttcttt ttcggcctat aaatcaaccg catccggcat ttattggcgg
     2941 atgcgatgct gccgtatctt atccacccta caagccatga accgtagacc ggataagcgc
     3001 agcgcatccg gcagttatgc cgcacgttca tcccgcaccg ccaacacctc gggcaaattc
     3061 aactcaatcc agtccgccag tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg
     3121 aggctatatt ccacatgcgg cggcaccacc ggatacgcga tacggttaag aaaaccatcc
     3181 tgttctaacg cctgtaacga ctgcgcaagc atcttttcac tcaccccacc gattttgcgc
     3241 cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa caccccccaa
     3301 cggctggtga cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa gagattaccc
     3361 tctttcagtt gttgtgacag gctaacctga ctcatttcat acttaccttt ttgcatgtac
     3421 ttactaaaag taagtttagg tgttagcgta tttaaacaca agacaaaacg atggagactt
     3481 cccatgatcg ctattactgg tgccactggc caacttggtc actatattat tgaatccttg
     3541 atgaaaacgg ttcctgccag ccaaatagtg gctatcgttc gtaatccggc aaaagcccag
     3601 gccctggcag cacaaggcat taccgtgcgt caggctgact acagcgatga agccgcactg
     3661 acatctgcac ttcagggagt ggaaaaacta ctgctgatct cttccagcga agtgggtcaa
     3721 cgtgccccgc agcatcgtaa tgttattaat gccgcaaagg cggctggcgt gaaatttatc
     3781 gcttatacca gcctgctaca tgcagatacc tccccgctcg gcctcgccga tgagcacatc
     3841 gagacggaga aaatgctggc tgattctggc atcgtttaca ccctgctgcg caacggctgg
     3901 tacagcgaaa actacctcgc cagcgccccg gcagcactgg aacacggcgt atttatcggt
     3961 gcggcgggcg atggcaaaat cgcctcagca acgcgggcag attatgcgac agctgcggca
     4021 ctcgtgatta gcgaagccgg tcacgaaggc aaggtttacg aactggcggg cgatagtgcc
     4081 tggacgttga cacagttagc ggcagagctg accaaacaga gcggcaaaca ggttacctat
     4141 caaaatctga gcgaagccga tttcgccgcg gcactgaaaa gcgtcggact gcccgacgga
     4201 ctggcggata tgctggcgga ttctgacgtt ggcgcatcga aaggtggtct gtttgataac
     4261 agcaaaacgc ttagcaaatt gattgcccgc ccaacgacaa cgttagccga aagcgtaagc
     4321 catcttttta atgttaataa ctagttaatt aaagtggcat cctcccgcat cctctctgat
     4381 aatgacggga tgccgggagc aatcatgtct gcttcctgaa cgtttcttct gacagaccaa
     4441 tggatgccag taatgattag cggcgtgctg tacgccctgt tagcagggtt gatgtggggg
     4501 cttatttttg tcgggccgtt gatcgtgccg gaatacccgg cgatgttaca gtcgatgggg
     4561 cgttatctgg cgttagggtt aattgcgcta cccattgcct ggctgggacg cgtgcgtctg
     4621 cgtcagttgg cgcgtcggga ctggcttacc gccttgatgc tcactatgat gggcaacctc
     4681 atttattact tctgccttgc cagtgccatt caacgtactg gcgcgcctgt ttccacgatg
     4741 attatcggca ccctgccggt ggtgattcct gtctttgcca atctgcttta tagccagcgc
     4801 gacggcaaac tcgcgtgggg aaaactcgcc ccggcactga tttgtattgg catcggcctg
     4861 gcgagtgtga atattgctga gttaaaccac ggactccccg attttgactg ggcacgttat
     4921 acctctggca tcgtgctagc gttagtttcc gtggtctgct gggcatggta tgccctgcgc
     4981 aacgcccgct ggctgcggga aaatcccgac aaacatccga tgatgtgggc gacggcgcag
     5041 gcgctggtca cgctgccggt ttctctcatc ggctatctcg tcgcctgtta ctggctgaat
     5101 atacaaacgc cggacttctc cttacctttt ggcccccgtc cgctggtgtt tattagtctg
     5161 atggttgcga tagccgtgct ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc
     5221 cagcgattac cgacagtgat tctcgggccg ctgattgttt tcgaaacgct ggcaggtttg
     5281 ctgtacacct ttttactccg ccagcaaatg ccgccgctaa tgacgctgag cggtatcgcg
     5341 ctgttagtga ttggcgtggt catcgcggtc agagcaaaac cagaaaaacc tttaactgaa
     5401 tctgtctcag aaagttgaca tgctggcagt gagttaaata agcctctgct acgtaagggt
     5461 tatagctttt gccttaaagg tgtatttaaa atacatctta tcttattaag aatgaggtat
     5521 cagctatggc ttatcgcgac caacctttag gtgaactggc gctctctatc cctcgcgctt
     5581 cggctctgtt tcgtaaatat gatatggatt actgctgtgg cggtaagcag acgctggcgc
     5641 gcgcggcggc acgtaaagaa ctggatgttg aggtcattga agctgaactg gcaaagcttg
     5701 ctgaacaacc gattgagaaa gactggcgta gcgccccgct ggcagaaatc attgaccata
     5761 tcatcgtgcg ctaccacgat cgtcaccgcg agcagctgcc ggagctgatc ctgcaagcga
     5821 ctaaagtcga gcgcgttcac gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc
     5881 tgaccatgct gcatgaagag ctttccagcc acatgatgaa agaagagcag atcctcttcc
     5941 cgatgatcaa acaaggcatg ggcagccagg caatggggcc aatcagcgta atggaaagcg
     6001 agcacgatga agcgggcgaa ctgctggaag tgatcaaaca cactaccaat aacgtcacac
     6061 cgccgccaga agcctgcacc acctggaaag cgatgtataa cggcattaat gaactgattg
     6121 atgacctgat ggatcacatc agtctggaaa acaatgtact gttcccacgc gccctagcgg
     6181 gtgagtgatc taaaagctgg atggcattgc gccatccagc atgataatgc ggttatttcc
     6241 gcagttcagc agcccgcttc ttaccaataa acagccagcc caaccccagc gcgataaacc
     6301 acagcggggt aaccagcagc gcctggcgag tgtcatcttc cagtgtcagc aacacgacca
     6361 cgaacacaaa gaacgccata catacccagc acatcagctt gccgagcggc atcttgtaga
     6421 tcgacttttc atgcagatga ggacgctgtt tgcggtacac caggtacgag caaaggataa
     6481 tcgtccagac gaacataaac agaatcgcgg aaacggttgt aatcatcgtg aacgcgccaa
     6541 tcacgctagg attcacatac aacatcacca cgccgccgag cagacagata cacgagaagg
     6601 tcaacccttt cgcgggtact gcgcgcttag aaagtttagc gaacgctttc ggcgcaacac
     6661 cttcctgcgc cagaccaaac agcatacggc tggtagagaa gacaccgctg ttagcggaag
     6721 acgccgcaga ggtcagcacc acaaagttga tcacgctggc agccgcaggc aaacctacca
     6781 acacaaacag ctcaacgaac gggcttttct ccgggactac cgaactccac ggtgtcacgg
     6841 acataatcac aatcagcgcg aagacgtaga acataatgat acggatcgga atggagttaa
     6901 tcgcgcgtgg cagtgatttc tccggatctt tggtttccgc agctgttgta cctaccagct
     6961 caatccccac gaaagcgaaa accgctatct ggaatccggc aaagaagcca cttaaacctt
     7021 tcgggaacca gccgccgtca ttccacaaat gcgcgaatga cgcttccaca ccggtcggtg
     7081 actgaaagtg catcgccacc atgaccaggc cgacgacaat cagcgacacg atggcgacga
     7141 ttttgatcat cgcaaaccag aactccatct caccgaacat tttcacggtg gcaaggttga
     7201 gcgtcagcag cagcactatc accgccagcg aggcgaccca gtcggagaaa tcggggaacc
     7261 agaactgagc ataagccgtg atcgccacca cgtctgccat accggttaca acccagcaga
     7321 accagtaagt ccagccggtg aaatatcccg cccacggccc cagtaaatcg gaagcgaagt
     7381 cactaaaaga tttgtattcc agattcgaaa gcagcaattc ccccattgcc cgcatcacga
     7441 aaaagagcat aaaaccaatg atcatataaa cgaaaatgat cgacggcccg gcaaggctaa
     7501 tcgttttgcc ggaccccata aacaacccag taccaatggc accgccaatg gcaataagct
     7561 gaatatgtcg gtttgtgaga ttgcgccgta gcgactgttc agccggagcc tgatcatcgg
     7621 caacgacttt tacctgatct accatgtttt ttcttcctgt acctgtctgt gttgttcagg
     7681 ctctacggcc tttagtcagt ctatgatacg acgaatctgt atcgggactc atcgatatta
     7741 ggtaacaata cgcgggatga atactaagat ttagatctaa tgttaatttt atgtttaaag
     7801 tgagtgtcat atcacggtga taacgcgaaa cagctcacaa aaatacacgc ttacggggtc
     7861 tttgcaacac aaaatagcgc cgtttggcaa actgaagggt ttattgctga atgcctgctc
     7921 ccctctggtt cgaggggaac agaatgcgct gcttagagga tttccagcag ttcgacttca
     7981 aacaccaggg tgctgaacgg agggatggat gcacctgcgc cgcgctcgcc atatgccagt
     8041 tcctgcggga tagtcagttc ccatttagaa cctaccggca tcagagtcag tgcttcaatc
     8101 cagccaggga tcacgccatt aaccgggaat tcagcgggtt caccacgagc aacggagctg
     8161 tcaaacacgg tgccgtcgat cagtttaccg gtgtaatgaa cacgaacgcg gtcggtacgt
     8221 gccggaattg cgccttcacc ctggttgatc acgcggaatt gcaggccaga ttcggtgcta
     8281 ttcacacctt cttttttggc gttttcttcc aggtatttca caccttcagc agccatcgcc
     8341 tggaaacgct gacgacgaac ggcatcggcg cgttcgtgga tttcacgcag cgcgcgatgc
     8401 accacatcaa ccggcacagc cggatgtttg ccttccagcg catcggcaat acctgcaacc
     8461 agtgcttctg gcagcagccc ttccaggcca gattcactca gttgttgccc tacctgcaaa
     8521 ccaatgccgt agcttgcttg cgcttcgatg gtgtcaaaag ttggggtggt catcgtattt
     8581 cctttcttag ggtataaaag tagcgggcag catatcagcc acacttcttt gggtaaaact
     8641 ttgtcgcagg aatgatgaca aatcccgggg aaaaagaaac aatagcaata tcccgtatga
     8701 atataataaa ggcgggttgg taatgctgcc aacttactga tttagtgtat gatggtgatt
     8761 ttaaggtgct tgcgt
//
LOCUS       DADMFL010000148         8674 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.151, whole genome shotgun sequence.
ACCESSION   DADMFL010000148 DADMFL010000000
VERSION     DADMFL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.151"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..323)
                     /locus_tag="JNZ27_003595"
     CDS             complement(<1..323)
                     /locus_tag="JNZ27_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8665773.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(333..881)
                     /locus_tag="JNZ27_003596"
     CDS             complement(333..881)
                     /locus_tag="JNZ27_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY8665774.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(881..1588)
                     /locus_tag="JNZ27_003597"
     CDS             complement(881..1588)
                     /locus_tag="JNZ27_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8665775.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JNZ27_003598"
     CDS             complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JNZ27_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY8665776.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2285..2995)
                     /locus_tag="JNZ27_003599"
     CDS             complement(2285..2995)
                     /locus_tag="JNZ27_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8665777.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JNZ27_003600"
     CDS             complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JNZ27_003600"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY8665778.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4414..5634
                     /gene="astC"
                     /locus_tag="JNZ27_003601"
                     /pseudo
     CDS             4414..5634
                     /gene="astC"
                     /locus_tag="JNZ27_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5631..6464
                     /gene="astA"
                     /locus_tag="JNZ27_003602"
                     /pseudo
     CDS             5631..6464
                     /gene="astA"
                     /locus_tag="JNZ27_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6460..7389
                     /locus_tag="JNZ27_003603"
                     /pseudo
     CDS             6460..7389
                     /locus_tag="JNZ27_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            7386..>8674
                     /gene="astB"
                     /locus_tag="JNZ27_003604"
     CDS             7386..>8674
                     /gene="astB"
                     /locus_tag="JNZ27_003604"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY8665779.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2022 a         2226 c         2384 g         2042 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg
      421 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc
      481 agggcatcat cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc
      541 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
      601 gtgctgccgg tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
      661 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt
      721 ttgcgctcca gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc
      781 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
      841 cgtaaaatga aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc
      901 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
      961 gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa
     1021 tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
     1081 tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca
     1141 ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc
     1201 cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
     1261 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
     1321 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
     1381 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
     1441 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
     1501 gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
     1561 agattttacg cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa
     1621 ggtaaccgtt gccagcgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac
     1681 ttcgccgcgt ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg
     1741 gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc
     1801 gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata
     1861 cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt
     1921 cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc
     1981 ttcataaaat gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc
     2041 atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac
     2101 cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc
     2161 gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac
     2221 agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat
     2281 cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca
     2341 catgtttgtg tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg
     2401 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     2461 tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc
     2521 agaaggcgat ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     2581 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     2641 ggaaggtatg gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     2701 gcaggaatga gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga
     2761 gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg
     2821 gggcattgat gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga
     2881 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     2941 aaatgaccag cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg
     3001 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     3061 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     3121 ttaattctcc tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc
     3181 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     3241 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg
     3301 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     3361 ttagcatggc ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc
     3421 tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca
     3481 atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta
     3541 tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc
     3601 gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg
     3661 acataaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg
     3721 tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca
     3781 taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc
     3841 ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt
     3901 tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca
     3961 aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg
     4021 cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg
     4081 gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta
     4141 aatttcgtca atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt
     4201 ttattctttt tattcaccca catcgcaaac gtattcattt tatatgcact ttaaatgcat
     4261 atggtttggt tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca
     4321 ctggctgaca cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat
     4381 acaaataacg aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac
     4441 tttgatgaat ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa
     4501 ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg
     4561 gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt
     4621 aagttctggc ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa
     4681 ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac
     4741 gaagcggcgc taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt
     4801 ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt
     4861 gggcagccag cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca
     4921 tataacgata ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc
     4981 gagcccatct agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg
     5041 cgtgaattgt gtgaccgcca caatgccttg ctgatttttg atgaggtaca aaccggcgtc
     5101 gggcgcactg gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc
     5161 accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc
     5221 gcccgcgtga tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg
     5281 gcggtagcag gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa
     5341 cagcgtcacg actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc
     5401 agtgaagttc gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg
     5461 caagcgaaac agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt
     5521 ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga
     5581 ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca
     5641 tccgtcccgt tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg
     5701 gcggcctgac gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa
     5761 tcaaaacctg gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag
     5821 atagcgagac aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg
     5881 atccctggta caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct
     5941 ataacgcatt gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca
     6001 cgctgtttct cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc
     6061 gctttatgtt tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc
     6121 gcggggtgat tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct
     6181 tttcgatgga ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg
     6241 cagaactgat gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg
     6301 tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt
     6361 ttcgttaccg caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg
     6421 accgcgtgcg cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg
     6481 ccgggcgtgc tgaacctggt gcagggcggg cgtgaaacgg gtcaggcgct gagtgcgctg
     6541 gaggatctcg acggtttgct gtttaccggt agcgccaata ccggctacca gctgcatcgc
     6601 cagctctccg gtcagccgga gaaaattctc gcccttgaga tgggcggtaa taatccgcta
     6661 attatcgatg aggtggcgga tatcgacgcg gctgtccatc tgaccattca gtcggcgttt
     6721 gtcacagccg ggcaacgctg cacctgcgcc cgccgtttat tgctcaaaag cggagcgcag
     6781 ggcgatgcgt ttcttgctcg tctggttgcc gtcagccagc gattaacgcc gggcaactgg
     6841 gatgacgaac cgcagccgtt tattggcggg ctgatttctg aacaggccgc acagcaggtg
     6901 gttactgcct ggcagcaact ggaagcgatg ggcggacgaa ccctgcttgc gccgcgctta
     6961 ttacaagcag ggacatcgtt gctgacacca gggatcattg aaatgacggg cgttggcggt
     7021 gtgccggatg aagaggtgtt cgggccgctt ttgcgcgtct ggcgttatga tacttttgat
     7081 gaggcgattc ggatggcgaa caacactcgc ttcggactct tttgcggtct ggtttccccc
     7141 gagcgggaaa aattcgatca actgttgctg gaggcgcggg cggggattgt taactggaac
     7201 aaaccgctta ctggtgctgc cagtaccgcg ccattcggcg gcattggtgc ttccggtaac
     7261 catcgcccca gcgcctggta tgccgcagat tactgcgcat ggcctatggc gagcctggag
     7321 tcggactcgt taacattgcc cgccacgctt aaccccgggc tggatttttc cgatgaggtg
     7381 gtgcgatgaa cgcctgggaa gttaatttcg acgggctggt agggctgacg catcattacg
     7441 cgggcctgtc gtttggtaat gaagcctcta cccgtcaccg ttttcaggtg tctaacccgc
     7501 gactggcggc aaaacagggc ttactgaaaa tgaaagccct tgccgatgcg ggattccccc
     7561 aggccgtgat cccgccgcac gagcgtccgt ttattccggt gctgcgtcag ttgggattca
     7621 gtggtagcga tgagcaggta ctggaaaaag ttgcacgcca ggcaccgcac tggctttcca
     7681 gcgtcagttc cgcttcgcca atgtgggtag ccaatgcggc aacgatcgcg ccatctgccg
     7741 atacgctgga tggcaaagtg catctcaccg ttgccaacct gaacaataaa tttcaccgtt
     7801 cgctggaagc gcccgtcact gaatcgctgt taaaagcgat ttttaaggac gaagagaaat
     7861 ttagcgtcca ttcggcgttg ccgcaggtag cgttgctcgg tgatgagggg gcggcaaacc
     7921 acaatcgtct cggcggtcat tacggtgaac cgggtatgct actttttgtc tacgggcgag
     7981 aagaaggcaa tgatacccgg ccttcccgtt atccggcgcg acagactcgc gaagccagcg
     8041 aggcggtggc aaggcttaat caggtgaatc cccaacaggt gattttcgcc cagcaaaacc
     8101 cggacgttat cgaccagagc gtttttcata atgacgtgat tgccgtgagt aaccgccagg
     8161 tgctgttttg ccaccaacag gcgttcgctc gccagtcaca gttactggca aacctgcggg
     8221 cgcgggtgaa cggttttatg gcgatagaag ttccggcaac tcaggtttcc gtgtcggatg
     8281 cggtgtctac ctatctgttt aacagccaac tgttgagccg cgatgatggt tccatgatgt
     8341 tggtgctgcc tcaggagtgt cgggaacacg ccggagtatg gggttatctc aatgaactcc
     8401 ttgccgctga caacccgatt agcgaactaa aagtctttga cttacgtgaa agcatggcga
     8461 atggcggcgg cccggcgtgc ctgcggttgc gggtggtatt gacagaagaa gaacgccggg
     8521 cggtgaatcc ggcggtgatg atgaacgata ccttgtttaa tgcgctcaat gactgggtgg
     8581 atcgttacta ccgcgatcgt cttactgctg ccgatctggc cgacccgcaa ttgctgcgcg
     8641 aagggcggga agcactggat gtattgagcc aatt
//
LOCUS       DADMFL010000149         8330 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.152, whole genome shotgun sequence.
ACCESSION   DADMFL010000149 DADMFL010000000
VERSION     DADMFL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.152"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..892)
                     /locus_tag="JNZ27_003605"
     CDS             complement(<1..892)
                     /locus_tag="JNZ27_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY8665780.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGS"
     gene            952..1230
                     /locus_tag="JNZ27_003606"
     CDS             952..1230
                     /locus_tag="JNZ27_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665781.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNZ27_003607"
     CDS             complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNZ27_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY8665782.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNZ27_003608"
     CDS             complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNZ27_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY8665783.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3384..3716
                     /gene="hdeA"
                     /locus_tag="JNZ27_003609"
     CDS             3384..3716
                     /gene="hdeA"
                     /locus_tag="JNZ27_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY8665784.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3832..4158
                     /gene="hdeB"
                     /locus_tag="JNZ27_003610"
     CDS             3832..4158
                     /gene="hdeB"
                     /locus_tag="JNZ27_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY8665785.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4222..4868
                     /locus_tag="JNZ27_003611"
                     /pseudo
     CDS             4222..4868
                     /locus_tag="JNZ27_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4910..5440)
                     /locus_tag="JNZ27_003612"
     CDS             complement(4910..5440)
                     /locus_tag="JNZ27_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8665786.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNZ27_003613"
     CDS             complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNZ27_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY8665787.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6409..7632)
                     /locus_tag="JNZ27_003614"
     CDS             complement(6409..7632)
                     /locus_tag="JNZ27_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665788.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDASLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2426 a         1615 c         1733 g         2556 t
ORIGIN      
        1 ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt
       61 tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atcaaccgtc gggtcggaga
      121 atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg
      181 tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat
      241 cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg
      301 aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc
      361 ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg
      421 ctttggcgac ggtgacattg gcttctgctt cattcagctg ggtgcgcgcg gtgtcgtaat
      481 cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg
      541 cattgctggc ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct
      601 gtaaaggtgc aggatcaatc tgatacagcg aatcgctctg gttcacttta tcgccttcga
      661 taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca gcttcataag
      721 gaacggttct accaggcaat tcgctcaaca cattgaccga actaggggag agtgtgacga
      781 caccgacctc aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga
      841 gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc
      901 ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat
      961 ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag gcacagtgat
     1021 cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc
     1081 attcgggcaa cgaagggttc tactggtgga tacacatacc aggggaatca agtcgtttta
     1141 gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc
     1201 agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac
     1261 gatgtcgctc atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa
     1321 tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat
     1381 cagttgcata attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa
     1441 gtgtttacca taagtaatct caagaagatt tttgatactt tctttgcggc taacaaaata
     1501 aataggcgct ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc
     1561 ggcatctaat ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt
     1621 atcaatgata accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat
     1681 ttttatcatt tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt
     1741 cgtcatgaga aaaatcataa cttgctcctt agccgttatt gtttcgaaca tgtcatcctt
     1801 ttcattattt acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt
     1861 tgcccttcgt gaactaatac atctattaac actaatcaat acttcagatt tatccatcac
     1921 gcactccata tattgatcct aagtaagatt cctggttgtt atcagcttgt aaatagtaaa
     1981 caaaaagtta acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga
     2041 tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt
     2101 ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag
     2161 taaataacgc atttatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg
     2221 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
     2281 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
     2341 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
     2401 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
     2461 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
     2521 atcaagtgac tatgatccgg gtgacaaccg gggtaattat tgctgcttaa cgaacaaact
     2581 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
     2641 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
     2701 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
     2761 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
     2821 gcccagctcc ggtgcacgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
     2881 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
     2941 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
     3001 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
     3061 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg ccttatctat
     3121 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
     3181 aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg
     3241 catctgtaac tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg
     3301 acatatacag aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc
     3361 actttatagt tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc
     3421 ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac
     3481 cggtcaactc atggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg
     3541 cagttggttt tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg
     3601 ttcagggtat tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag
     3661 ccaactttaa agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc
     3721 gggaatgcgt tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa
     3781 gcagctattc ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt
     3841 tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac
     3901 gcacaatctg cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt
     3961 gatctgaatc caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta
     4021 tataaaggtg gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg
     4081 atcgaatact gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct
     4141 aatgacttgc cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc
     4201 tgctcctttt tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca
     4261 attgcctgtg gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc
     4321 acacatgtat taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt
     4381 gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg
     4441 gtgacgggcg tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta
     4501 ggtctgacga cggcagcgga tatctgggtg accgccgcat aggtatggtt attggcagcg
     4561 gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc
     4621 atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg
     4681 gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaact gatttagttt
     4741 cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa
     4801 cgttggtaga agacctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa
     4861 ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat
     4921 aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
     4981 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
     5041 cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca
     5101 cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag
     5161 tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta atattaaagg
     5221 gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
     5281 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
     5341 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
     5401 cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc tggtgcggac
     5461 attaacttta taatcagttt gccaaaggta attaacagga cagggcttat aataataata
     5521 gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag aaagcatctg
     5581 catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg cattggtgta
     5641 gtaaggcgca cccaagcccg gttccggcca gaatgcacca tagccgtaat cccacaggtt
     5701 ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca tattcacttc
     5761 caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa tggtaccgag
     5821 gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt gtctggcgag
     5881 cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca acggtaatac
     5941 agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct tcccaccaaa
     6001 gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa aacttttttg
     6061 aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg cggcaagcaa
     6121 aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat ccttatcaac
     6181 aaatattaaa accataatag atatttcttt atagttttca tctgattctg aggtgatatg
     6241 cctgatagta acgtaaacct gtctgatgtt tatatccctt ctgtttagtt caattccgtt
     6301 attttggggc gtggtatgag tatccatcgc ccaataatat aaccctttgt tttaaaatta
     6361 cataaaatct atttttattc aatgattata aagtgctaat aatcaatatt atggtattat
     6421 attctgataa tgatttgata agcgaattct tttgccagat ggtatttcta tttccacccc
     6481 ataatttata tctttttcat taggcagatc tctctttaaa taaaaggggt atgaaatctt
     6541 ttcgggttga agtgtctcgt aatgaaatgc tatgaattca tttttagaca ttgttgccca
     6601 tttttgaatt ttattgtata gagagggtgt tgtaaactct tgaagttgtt tataacatat
     6661 ttttttgaac tcttgcttat attttaatat gtcatcaaat gaaagattca ctacctttgc
     6721 ctctaagaaa tcaattattc tcgaagcaat ttccgctttc gcgcgtaaag agttgtcatt
     6781 aatagttaac aatgtagaaa acaagccatt gatttgtttg tcaagatgtt gttcatattt
     6841 atgtagtttg gggatgggat tttccttgtg tgaaaaaata gtgttaaata aacttgcgtc
     6901 agtgaaattt atattaagta gatcttcaaa ataaaatttt gataaggtta ggactagctt
     6961 ggacatatct tgccctgtca tgttgaaatc aataaagtcg catccagcaa gggcttccat
     7021 cctattttca tttttactgt gctcaatata cagcttcgcc atttttaagt acaagctttg
     7081 aatgtctctt atatcggttg gtaagtcagc tcgttgccca gcaaaatttt tattatcgga
     7141 agaaacggtt tggacactga atctttcggg tattccttct tctacgagaa ttttagttat
     7201 gattggattg ttgggttctg gattaacgac aacaatgatt tgacatacag aatccttatc
     7261 cttttcgtat ctaatgttat gaatctgatt aagtattgat tgtgtctgtt gcgtaagtgg
     7321 tgtcgaactt atcgtttcaa tgttattaat aagacttaca agttgaacca caacttttga
     7381 gtagtctgca tttgtatatg tggtgttcgt gtggcatgca ttaatttgtt ggagttgttg
     7441 ttgcgctaaa tcaaattgtt cagggttgta tccttctcca gagaatgtgg aattaatggg
     7501 ttgttgttct tcccaattat cacaaggcca cttcggtgtg tctggcgttt taacattatc
     7561 ggtatttgtt gtgcagttat aaatatcgcc gtgatatgta tttattatac ctggggtaaa
     7621 gtcaattgac ataacattcc ttattcatca tctgttttat gctaatgttt ttaaattgga
     7681 taattagaac aggaatggat tttcttctac acacaataaa tataatgcgt tatatcttcc
     7741 tgatataatt acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa
     7801 agggcagtga tagtatcgta aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa
     7861 aaagttatta tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
     7921 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
     7981 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
     8041 gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg tcatcattct
     8101 caatgcatta gttaaatcat ttgtgatttt taaggattat cctgtctatg attttctact
     8161 tagaaggata tggcactaac tatgcatctt atagtttact gctggcatct atttaaaata
     8221 gcaattgagt caaatagccc atcaagatag ccatacctaa tataaccgtc aggaatgcag
     8281 ctatcatcgg cattctgaac attgatttaa gtaatatcac ttccgtcagg
//
LOCUS       DADMFL010000150         8321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.153, whole genome shotgun sequence.
ACCESSION   DADMFL010000150 DADMFL010000000
VERSION     DADMFL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.153"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            336..3185
                     /locus_tag="JNZ27_003615"
     CDS             336..3185
                     /locus_tag="JNZ27_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8665789.1"
                     /translation="MGIKQHNGNTKADRLAELKFRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NVLTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKVSRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3285..5213)
                     /gene="dhaR"
                     /locus_tag="JNZ27_003616"
     CDS             complement(3285..5213)
                     /gene="dhaR"
                     /locus_tag="JNZ27_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY8665790.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGERTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5432..6502
                     /gene="dhaK"
                     /locus_tag="JNZ27_003617"
     CDS             5432..6502
                     /gene="dhaK"
                     /locus_tag="JNZ27_003617"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY8665791.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6513..7145
                     /gene="dhaL"
                     /locus_tag="JNZ27_003618"
     CDS             6513..7145
                     /gene="dhaL"
                     /locus_tag="JNZ27_003618"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY8665792.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7156..>8321
                     /gene="dhaM"
                     /locus_tag="JNZ27_003619"
     CDS             7156..>8321
                     /gene="dhaM"
                     /locus_tag="JNZ27_003619"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8665793.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKE"
BASE COUNT         2170 a         2069 c         2077 g         2005 t
ORIGIN      
        1 atcccataac taattaggat tattacaaaa aatcttcacc aaatttttta accgctgttt
       61 gttgtaatat ttttgccata ttcatagtaa cgaattggaa taagtaacga caacttaggg
      121 aataactcga tataaactca cctttgtaag gggggtgtta aatatatatt aattcctgtg
      181 ttcctgattt tgcccggtaa tagagaatta ttctcagaga agctcatcat gattgcacca
      241 tacagatatc atgatgataa gaggatatta ttcattgact taaacaaaat gacaaaatcc
      301 atagacccat agcctgggta ctatatagga gtggtatggg catcaaacaa cacaatggga
      361 ataccaaagc cgatcgtctc gctgaattaa aattccgttc gccctcaatt caactgataa
      421 aatttggcgc tattggtttg aatgcaatta tcttttcccc cctgctgata gctgctgata
      481 caggaagtca atatggcacc aatattacta ttaatgatgg tgacagaatt acaggagata
      541 ccgccgatcc atcaggaaac ctctatggtg taatgacccc agcaggaaac acgcctggca
      601 atatcaacct gggtaatgat gtcaccgtca atgtcaacga cgcctctgga tatgcaaaag
      661 gaatcattat tcagggcaaa aacagctccc tgacagctaa ccgactcaca gtagatgttg
      721 ttggtcaaac ctctgccatc ggcattaact taattggtga ctatacccat gctgacttag
      781 gcacaggcag caccattaag agtaacgatg acggcatcat tattgggcat agctcaacac
      841 taacagccac tcaattcacc attgaaaact cgaacggtat aggcctaacc atcaatgact
      901 atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa gaccgatgga agtacaggtg
      961 tttatatcgg tggtctcaac ggcaataacg ccaatggtgc tgcgcgtttt acggcgacag
     1021 acctgacaat cgatgttcag ggctacagcg ccatggggat aaacgtacag aaaaactctg
     1081 ttgtcgatct cggaacaaac agtaccatta aaaccaatgg cgataatgct cacggcctct
     1141 ggagctttgg ccaggttagc gcgaatgtac tcactgttga tgtaactgga gccgcggcca
     1201 atggcgtcga agttcgtggt ggtacaacca ctatcggtgc agatagccat atttcttccg
     1261 cgcagggcgg tggcctcgtc accagtggtt cagacgcgac aatcaatttt tctggcacgg
     1321 cagcgcaacg aaacagcatc ttttccggcg gttcttatgg tgcctcggcc cagacggcaa
     1381 cggctgttat caacatgcaa aataccgata ttacggttga tcgtaatggc agtctggcgc
     1441 tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga cagtttggct atcaccggcg
     1501 cggcaggagc cagagggatt tatgccatga ccaacagcca gatcgacctc acgagcgatc
     1561 tggtcattga tatgagtaca cccgaccaga tggccatcgc aacgcaacat gacgatggtt
     1621 atgccaccag ccgcatcaac gcctcgggtc gtatgcttat caacggtagc gttctttcca
     1681 aaggtgggct aatcaatctg gatatgcacc ctgggtcggt ttggacaggt tcctccctca
     1741 gcgataatgt caatggcggg aaactggacg ttgcaatgaa taacagcgtc tggaacgtaa
     1801 caagtaattc taatctcgac acgctggcgc tgagccattc aactgtcgat tttgccagcc
     1861 acgggtcaac tgccggcaca tttaccacat taaacgtaga gaacctgagc ggtaacagta
     1921 cctttattat gcgtgctgat gttgttggcg agggtaatgg cgttaataat agaggggatt
     1981 tattgaatat cagcgggagt agtgctggta atcacgtatt ggctatccgc aaccagggca
     2041 gcgaggccac aacgggaaat gaagttctga cagtggtaaa aaccactgac ggcgcggcct
     2101 cgttcagcgc gtcttctcag gttgagttgg ggggatatct gtacgatgtg cgtaaaaatg
     2161 gcactaactg ggagctttac gcttccggga cagttccgga accgactcct aatcctgaac
     2221 ccacaccagc tcccgctcag cctcccatag tcaaccccga tcctacgcct aaacccacca
     2281 cgaccgcaga tgctggcggc aattatctca atgtcggtta cttattgaac tatgttgaaa
     2341 accgtacgct gatgcaacgg atgggtgact tgcgaaatca gagtaaagac ggtaatatct
     2401 ggttgcgcag ttatggggga agcctggact cctttgccag tggcaaactg agcggctttg
     2461 acatgggtta cagcggtatc cagtttggtg gggataaacg tctctctgat gtaatgccgt
     2521 tgtatgtcgg tctgtatatt gactcaacac atgcatcgcc ggactatagc ggaggcgacg
     2581 gtaccgcacg ttcagactac atgggaatgt acgccagtta catggcacaa aacggttttt
     2641 acagcgatct cgttataaaa gtatcgcgcc agaaaaatag tttccacgta ctggacagtc
     2701 agaacaactg cgttaacgcc aacggcactg cgaatggaat gagcatctcc ctggaagccg
     2761 ggcagaggtt caacctgtcc cctactggtt atgggttcta tatagagccg caaacccagc
     2821 ttacatacag ccaccagaat gagatggcta tgaaggcgag taatggcctc aatatacatc
     2881 tgaatcacta cgaatcgctg ctggggcgtg ccagcatgat actggggtat gacatcaccg
     2941 caggcaacag ccagctgaat gtctatgtga agactggcgc tatccgcgag ttttcagggg
     3001 ataccgaata tctgttgaac gactcccggg agaagtacag tttcaaaggt aatggctgga
     3061 ataacggcgt gggagtcagt gcacagtata acaaacagca cacattctat ctcgaagcgg
     3121 attacacgca gggtaacctc tttgatcaga agcaagtcaa cggaggatat cgcttcagct
     3181 tttaagtgcg ggatagacaa gcccttaaac gatgctgaaa attgttagcg tgcgccggaa
     3241 tgaaatacag atcactctga tgtatcagaa atcgtttctg tctttcatac ccggcgctta
     3301 aactgccctg catcaatgcc atgttgcttc attttccgcc acagcgtagt gcggccgatc
     3361 ccaagtaaag ccgacatttc ctgaatgcga ccgcctgtga cctgggctgc gttaataatt
     3421 gcctcttttt caacttccgc aaatgacaga ctggtggaaa ggcgggtggc gctgacatca
     3481 tctgtcgcct gctcggtaaa cagatgttca ggtaaatcac tgacgcgaat gcgcccgtta
     3541 tcactactca gagcaagatt ctcgatgacg ctgtaaagtt caaaatcgtt gcctggccat
     3601 gcacaagaaa ctaggcgagc gagggcatcg tcatcaattt tcagccgcgt agagaagcgt
     3661 ttttcaagac tgcgtaattt gttattcacc agcgccggaa tgctgccacg ccgcatacgc
     3721 agaggcggga tggtaatttc aaatgcatgc agcgcgtaat acagctggcg actaaaacga
     3781 ttttgttcca ccagcattgc gaggtccgcg gtcgttgttg caatcacttt gacatcaatt
     3841 ggtattaaac gccgcgcatc cagtcgcgtg ataaccccct gcttgataac ctgcagcaaa
     3901 gcagactgta actccaccgc cagatattca atcttttcaa gaaacagcgt gccgccgtgt
     3961 gccagttcca gccgactcag acggccattt tcattgtccg tgcgctcgcc accaataaat
     4021 tcttccgcca gcgcagcatc accatataac tcacaattga cggcgatata aggacctgca
     4081 gcacgctcgc tttcattatg aattgcctga cttagcagtg ccttgcccac gccctcttct
     4141 ccacaaagca ggacaggaaa gctactgcgc gccgcctggc gaccaaaatg aatcaagcgg
     4201 cgggtttgcg gatcgtcctg tggcatatga gcgaaggtat ggctgacttt tcctaattga
     4261 ctggtcatta actgccgcat ctgttccaca ggatggagca acaaaataaa gctggttccc
     4321 tgcgtttcta ttatcggttt aagggttatc accgcatcaa taaactggtg ctggctttca
     4381 aaggttgctt ctacgtgttt gagcggatgt gcctgtttta ttgcttgttg caatacggcg
     4441 ggtaacgtta agagttcagt gattgcccgt ccctgacttg ccgtcgcgtc aaggcgcaag
     4501 acccgcgccg cctgggcatt aataaattgc aaattaccct gctcgtccca gctaatcacg
     4561 ccatcatcca tactttctaa cagggcatta agttgattta aatgacggtt agtttcagcg
     4621 agcaaactgt ccgtcagcag taaattaccg acctcgcggg cgattgccaa cgtcaacggc
     4681 aaatcagctg cggtagtttg ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg
     4741 cccttgctgt caaacaacgg cgttgcacaa aaggcccagt tccagagtgc ctgtttgaaa
     4801 tgttgatcgg ccatcgtttt cacggcctga ccagagatag ccgctaacga tagcgcacaa
     4861 gtaccaataa ttccctcggc gcaatacgtg ccgtcattga atcccagtgc acttagctgc
     4921 tgcaaggttt gcggatcgcc attacggctg agaatgcagg cggtttcatc gaggataaac
     4981 agcgcacact ctcgcggtgc catatattcc caggcatctt ccagcgcagc ctgcccgagc
     5041 gtcagcatcg ctttcttacg ccgataaata gaagcaaatg tcacgccctg ggcctgatgt
     5101 ggtacgttcc atgtctcccg tttcatcagc ttattgcatc gctcccagga ggttgcaatt
     5161 aagggagata tgccccgacc atcgttgtta aaagcgccac tcatatcccg catcccttat
     5221 gacaaaaggt gttccggctc gcaattttaa gtattaatac acgtaaaagt ttcaatatgg
     5281 aacattcatt aaagcatatg tttcactatg aaacataatt tgggctaatt ttcttttccc
     5341 ttgcctgatg cacaatggat tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc
     5401 catgccctac cgtaattgct ggcgcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga
     5461 cgtactggac gaacaactgg caggactggc gaaagcgcat ccatcgctga cactgcatca
     5521 ggatccggtg tatgtcaccc gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc
     5581 gggtggcggc agcggacacg agccgatgca ctgtggttat atcggtcagg ggatgctttc
     5641 gggggcctgt ccgggcgaaa ttttcacctc accgacgccc gataaaatct ttgaatgctc
     5701 catgcaaatt gatggcggcg aaggtgtact gttgattatc aaaaattaca ccggcgatat
     5761 tcttaacttt gaaacagcga ccgagttact gcacgatagc ggcgtaaaag tgaccactgt
     5821 ggtcattgat gacgacgttg cggtaaaaga cagtctttat accgccgggc gacgcggcgt
     5881 tgccaacacc gtattaattg aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact
     5941 ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc
     6001 tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct tttacccttg cggataatga
     6061 gatggagttt ggcgtcggca ttcatggtga gccaggtatt gaccgccgcc ctttctcttc
     6121 ccttgatcaa accgtcgatg aaatgttcga caccctgctg gaacatggct cataccatcg
     6181 cactttgcgt ttctgggatt atcaacaagg cagctggcag gaagaaccac aaaccaaaca
     6241 accgctccag tctggcgatc gggtgattgc actggttaac aatcttggcg caactccgct
     6301 ttctgagctg tacggcgtct ataaccgcct gaccacacgt tgccagcaag cgggattgac
     6361 tatcgaacgt aatttaattg gcgcgtactg cacctcactg gatatgaccg gtttctcaat
     6421 caccttactg aaagttgatg acgaaacgct ggctctctgg gacgccccgg tccacacccc
     6481 ggcccttaac tggggtagat aaggagaaag caatgtcact gagcagaact caaattgtta
     6541 actggctcac tcgttgtggc gatattttca acaccgagag cgagtatctt accgggctgg
     6601 atcgcgagat tggcgacgct gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg
     6661 tggaaaaact ccctgctatc gcagataaag atatcggttt cattctcaaa aataccggta
     6721 tgacgctgct ttccagcgtc ggtggtgcca gtggtccgct gttcggtacc ttctttatcc
     6781 gcgccgcaca ggcgacccag gcacggcaaa gcctgacact ggaagagctt tatcagatgt
     6841 tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc cgaacctggc gataaaacca
     6901 tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca gtccagcgag caaaatctct
     6961 ctgttccggt ggcgctcgaa gctgccagta gcatcgccga atccgcggca caaagtacga
     7021 ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg tgaacgcagt attggtcacc
     7081 aggatcccgg cgcgacctcg gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag
     7141 agtaaggaat tggtgatggt aaacctggtc atagtttcac atagcagccg actgggagaa
     7201 ggtgtcggtg aattagcccg tcagatgtta atgagtgata gttgtaaaat cgccattgcc
     7261 gcgggaattg acgatccaca aaatcccatt ggtaccgatg ccgtcaaagt gatggaggcc
     7321 atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga tggatatggg tagcgcatta
     7381 ttgagtgctg aaactgcgct ggaattgctg gctcccgaga tcgccgcaaa agtacgtttg
     7441 tgtgctgcgc cgttggtcga aggtacactg gcagcaacgg tcagcgcggc ctcgggggcg
     7501 gatatcgaca aagttatctt tgacgctatg catgcgctgg aagccaaacg tgaacaactg
     7561 ggtttaccgt cctccgacac tgaaatctct gacacatgtc ctccgtacga tgaagaagcc
     7621 cgttctctgg cggtggtcat aaaaaaccgt aacggcctgc atgtacgtcc ggcctcccgg
     7681 ctggtttata ccttatcgac atttaatgcc gatatgttgc tggaaaaaaa cggcaaatgc
     7741 gtcacaccag agagtattaa ccagattgcg ttactacaag ttcgctataa cgatacgctg
     7801 cgcctgattg cgaaagggcc agaagctgaa gaggcactga tcgctttccg tcagctggct
     7861 gaagataact ttggtgaaac ggaggaagtc gctccaccta ctctgcgtcc cgttctgcct
     7921 gtttcgggta aagcctttta ttatcaacca gttttatgta cggtacaggc aaaatcaacc
     7981 ctgaccgcgg aagaagaaca agatcgatta cgccaggcta ttgacttcac gttattagat
     8041 ctgatgacgt taacagcgaa agcagaagcc agcgggcttg acgatattgc cgcaatcttt
     8101 tctggtcacc atacactgtt aggtgatccg gaactgctgg cggcggcaag cgaactcctt
     8161 cagcatgaac attgcacggc agaatatgcc tggcagcaag ttcttaaaga acttagccag
     8221 caataccagc aactggatga tgaatatcta caagctcgct atattgatgt ggacgatctt
     8281 ctgcatcgca ccctggtcca cctgacccaa acgaaagaag a
//
LOCUS       DADMFL010000151         8316 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.154, whole genome shotgun sequence.
ACCESSION   DADMFL010000151 DADMFL010000000
VERSION     DADMFL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.154"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..157
                     /locus_tag="JNZ27_003620"
     CDS             <1..157
                     /locus_tag="JNZ27_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY8665794.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="JNZ27_003621"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="JNZ27_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY8665795.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="JNZ27_003622"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="JNZ27_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY8665796.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1292
                     /locus_tag="JNZ27_003623"
     CDS             873..1292
                     /locus_tag="JNZ27_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY8665797.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1279..6065
                     /gene="traI"
                     /locus_tag="JNZ27_003624"
                     /pseudo
     CDS             1279..6065
                     /gene="traI"
                     /locus_tag="JNZ27_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6085..6831
                     /gene="traX"
                     /locus_tag="JNZ27_003625"
     CDS             6085..6831
                     /gene="traX"
                     /locus_tag="JNZ27_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY8665798.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6886..7446
                     /locus_tag="JNZ27_003626"
     CDS             6886..7446
                     /locus_tag="JNZ27_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY8665799.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7577..7789
                     /locus_tag="JNZ27_003627"
     CDS             7577..7789
                     /locus_tag="JNZ27_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY8665800.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            7973..8272
                     /locus_tag="JNZ27_003628"
                     /pseudo
     CDS             7973..8272
                     /locus_tag="JNZ27_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
BASE COUNT         1911 a         2081 c         2660 g         1664 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg ggtgatgacg
     1261 gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt taaccacgac
     1321 accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa tgttacgcag
     1381 tataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg gttcattgag
     1441 aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact gaaagagcag
     1501 gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg ggaaatgccg
     1561 ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc cgtcggggaa
     1621 gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc caaacagcac
     1681 gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac cgggttcgac
     1741 atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca gacacccgga
     1801 cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat tgccggatta
     1861 agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt cggcatactg
     1921 ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgagct gtcagtccgg
     1981 gcactcagtc gtgacatcat gaaacagaac cgggtgaccg tacatccgga gaaaagtgtc
     2041 ccccggacgg ccggttacag cgatgccgtc agtgttctgg cacaggaccg tccgtcgctg
     2101 gccattgtgt ccgggcaggg cggtgcagcc gggcagcgtg agcgggtggc tgaactggtc
     2161 atgatggccc gggagcaggg gcgggaggtg cagattatcg ctgctgaccg tcgctcgcag
     2221 atgaacctga agcaggatga acggctgtcc ggtgaactga taaccggacg tcgtcagttg
     2281 ctggaaggca tggccttcac gccgggcagt accgttatcg tcgaccaggg cgaaaaactc
     2341 tccctgaaag agacgttaac cctgctggat ggtgccgcac gtcataacgt acaggtcctg
     2401 ataaccgaca gcgggcagcg aaccggtaca ggcagtgcac tgatggccat gaaggatgcc
     2461 ggggtgaaca catatcgctg gcagggggga gaacagcgac cggccaccat catcagtgaa
     2521 ccggaccgta atgtccgcta tgcccggctg gccggagatt ttgcggccag cgtgaaagcc
     2581 ggagaagaga gcgtggcaca ggtcagcgga gtacgggaac aggccatact gacacaggcc
     2641 attcgcagtg agctgaaaac acagggcgtg ctcggacacc cggaggtaac catgaccgcc
     2701 ctgtcaccgg tctggctgga cagccggagc cgttatctgc gggatatgta ccgcccggga
     2761 atggtgatgg agcagtggaa cccggagaca cgcagtcatg accgctatgt gacagaacgt
     2821 gtgacggcgc agagtcacag cctgaccctg cggaatgcgc agggcgaaac gcaagtggtg
     2881 cgtatttctt ccctggacag cagctggtcg ctgttccggc cggaaaaatg ccggtggcag
     2941 acggtgagcg tctgagggtg acagggaaaa ttcccggact ccgcgtctcc ggcggtgacc
     3001 gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt tgttgtgccg ggacgggctg
     3061 aaccggccac cctgcctgtg agcgattcac cgttcacggc actgaagctg gagaacggct
     3121 gggtggaaac gcccgggcat tccgtcagcg acagtgcgac ggtttttgcc tccgtcacac
     3181 agatggcaat ggacaatgcc accctgaatg gtctggcccg cagtggtcgt gatgtccggc
     3241 tgtattcctc actggatgaa acccgtactg cggaaaaact tgcccgccat ccctccttta
     3301 cggtggtttc tgagcagata aaggcgcgtg ccggtgagac attgctggaa accgctatca
     3361 gtctgcagaa agccgggctt cacacgccgg cacagcaggc cattcatctg gcccttcctg
     3421 tgctggaaag taaaaacctg gccttcagca tggtggacct gctgacagag gcgaagtcgt
     3481 ttgctgcaga aggaaccggt tttgctgacc tgggagggga aatcaatgcg cagataaaac
     3541 ggggtgattt actgtatgtg gatgtggcaa aaggctatgg cacaggcctg ctggtttccc
     3601 gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat tctcgaaggt aaggaggcgg
     3661 tcacgccgct gatggagaga gtacctggcg aactcatgga gaaactgaca tcaggacagc
     3721 gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt cacggtggtg cagggctatg
     3781 ccggtgtggg taagaccaca cagttccggg cggtgatgtc agccgtgaac atgctgccgg
     3841 agagtgaacg tccccgtgtc gtggggctgg ggcccacaca ccgtgcggtc ggggagatgc
     3901 gcagcgccgg cgtggatgca cagacactgg cgtcctttct gcatgacacg cagcagcgca
     3961 gcggagaaac gccggatttc agcaacacgc tgttccttct cgatgagagc tctatggtgg
     4021 gcaataccga catggcacgg gcatacgccc tgattgcggc cggtggcggt cgggctgtgg
     4081 ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg tcagcctttc cgtctccagc
     4141 agacgcgcag tgctgccgat gtggtcatca tgaaggagat tgtgcgtcag acgccggaac
     4201 tgcgggaggc ggtatacagc ctgattaacc gggatgtgga aagggcgctg tccgggcttg
     4261 agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc atgggcaccg gagcactccg
     4321 tgacggagtt cagtcacagc caggaagcga aactggcaga agcgcagcag aaggcgatgc
     4381 tgaaaggcga ggcttttccg gatgtcccca tgacactgta tgaagccatt gtccgcgact
     4441 ataccggcag gacaccggaa gcacgggagc agacgctgat tgtcacgcac ctgaatgagg
     4501 accggcgcgt actgaacagc atgattcatg atgcacggga aaaggccgga gaactgggga
     4561 aggtgcaggt catggtgcct gtcctgaaca cagcgaatat acgcgacggg gagctgcgtc
     4621 gtctctccac ctgggagaat aacccggatg cccttgccct ggtggataat gtgtatcacc
     4681 ggattgccgg tatcagcaag gatgacgggc tgataaccct gcaggatgcg gaaggtaaca
     4741 cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt cacgctgtac accccggaca
     4801 ccatccgggt ggggaccggt gacaggatac gcttcacgaa gagtgaccgg gagcgcggtt
     4861 atgtggccaa cagcgtctgg acggtgacag cggtttccgg tgacagtgtc acactttctg
     4921 acggtcagca gacccgtgtg attcgccccg gtcaggagcg ggcagagcaa catattgacc
     4981 tggcctatgc catcaccgcc cacggtgcac agggggcgag tgaaaccttt gccatcgcgc
     5041 ttgaaggcac ggaaggtaac cggaaactga tggccggctt tgagtcagcc tacgtggccc
     5101 tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa ccgtcagggc tggacggatg
     5161 ccatcaacaa tgccgtacag aaaggaactg cccacgatgt gtttgagccg aaaccggacc
     5221 gggaggtcat gaatgcagag cggctgttca gtacggcgcg ggagctgcgg gacgtggcgg
     5281 caggccgtgc cgttctccgt caggcggggc tggccggggg agacagtcct gcacggttta
     5341 ttgctccggg acgtaaatat ccgcaaccgt atgtggcact gccggcgttt gaccgtaacg
     5401 gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga tgacggaaac gggctgcggg
     5461 gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc gcagttcgtg gccctgcagg
     5521 gcagcagtaa cggagaaagc ctgctggctg ataatatgca ggatggcgtc cggattgccc
     5581 gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg tgaaggccgt ccgtggaatc
     5641 ccggtgccat caccggtggc cgcgtgtggg gggatatccc ggacaacagc gtccagccgg
     5701 gagccggaaa tggcgaaccg gtcacggcag aggtactggc acagcggcag gctgaagagg
     5761 ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt ccgtaaaatg gcagagaaca
     5821 aacctgacct gccggacggc aaaacagagc aggctgtcag ggaaattgcc ggtcaggagc
     5881 gtgaccgggc tgccataact gaacgggaag ccgcgctgcc ggagagtgta ctgcgtgaac
     5941 cacaacgggt gcgggaagcg gtccgggaag ttgcccggga aaatttgctg caggagcgtc
     6001 tgcagcagat ggagcgggat atggttcgtg acctgcagaa agagaaaacc ccgggcggag
     6061 actgatacag gaagacaaac actgatgaca acggataaca cgaacacgac acgtaacgat
     6121 tcactggctg cacggaccga tacctggttg cagtcatttc tggtctggtc acccggacag
     6181 cgggatatca tcaaaacggt ggcactggtg ctgatggtac tggaccacat caatctgata
     6241 ttccagctga agcaggaatg gatgtttctg gccgggcgag gagcctttcc gctttttgcc
     6301 ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc ggcaaccggc cattaaccgg
     6361 ctgtggggat ggggaattat tgcccagttc gcatattatc tggcaggctt tccttggtat
     6421 gaggggaata tcctgtttgc ctttgcagtg gcggcacagg tgctgacgtg gtgtgaaacg
     6481 cgcagtgggt ggcgtacagc agcggccata ctcctgatgg ccctgtgggg gcctttgtcc
     6541 ggcaccagtt acggtattgc cgggttgctg atgctggcag tcagcaaccg gctgtaccgg
     6601 gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc tgctggctgt tattccggcg
     6661 cttaaccttg ccaccagtga tgcagcggcg gtggccggtc ttgtgatgac ggtgctgacc
     6721 gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt tctggcccgg agactttttc
     6781 ccgacgttct atgcctgtca tctggctgtg ctgggcgttc tggcgctgtg acgggtgtgg
     6841 tatctttggc cgcaagagga tgattcgtca gaggcagaac acagcatgat agagcagaag
     6901 cgaccggtac tgacactgaa gcggaagaca gagggaacag cgcctgtccg cagccggaaa
     6961 accatcatca atgtcaccac gccaccaaag tggaaggtga aaaagcagaa gctggccgag
     7021 aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc aggccagaca ggcgctgtcc
     7081 atttatctga acctgcccac gctggatgag gctgtgaata ccctgaagcc ctggtggccg
     7141 ggattatttg acggtgacac gccccggctt ctggcctgcg gtatccggga cgtgttactg
     7201 gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa aactgcgcag ggcgctgaag
     7261 gccatcaccc gttcagaaag ctatctgtgt gccatgaaag ccggtgcctg ccggtatgac
     7321 acggaagggt atgtgacgga gcatatttct caggaggagg aagcgtatgc gggagcgcgt
     7381 ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac ttcaggccgt gcttgatgag
     7441 aaataaaaaa agcctccccc agagagagga ggcagggaaa taaggcttaa aaggaaataa
     7501 cttccgcttc cgaaaggaag ccagtccact gaattattta tcacaataat actgacaact
     7561 aaagggggac aagaaaatga atggattcag aaacagttca cggaacggcc aggtctggcg
     7621 ttaccagcgt gccggcgggc gggccgttat tctggaagtc agtggacgct ggatggaagc
     7681 ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg acagactggc agcagtttgc
     7741 ccggaaaagg gctgaacact gccaccggcg ctgccgggga agggtgtaac gaaaaaagca
     7801 gccttcatta ctctaggagt cagaaggctg aaaggagttt atgagatttg cttcttaata
     7861 tccggtcggg cagtatgacc ggacagttct gagtagcaca gagtcttttc cgcattaagt
     7921 cgatttttta tattttgtga ttaatttcac aaaataaggt gttattcaat gtgtgctgca
     7981 ctattcaggg ggtctgaaat acaggtggca tttatctgat atggagaata acatgaggaa
     8041 atatattcca ctggtattat ttatcttttc atggccggta ttgtctgcag atattcatgg
     8101 acgggttgtt cgtgtactgg acggtgatac catcgaggtc atggactcac tgaaggcggt
     8161 gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa caggattatg gccggtggtc
     8221 aacagacatg atgaaatcac tggtcgcagg aaaaacggtt accgtcacat attgaggtgt
     8281 actggcaata gcggacacta ccatttgttc tttttt
//
LOCUS       DADMFL010000152         8195 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.155, whole genome shotgun sequence.
ACCESSION   DADMFL010000152 DADMFL010000000
VERSION     DADMFL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.155"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNZ27_003629"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNZ27_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY8665801.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNZ27_003630"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNZ27_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY8665802.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNZ27_003631"
     CDS             complement(1088..2437)
                     /gene="chbC"
                     /locus_tag="JNZ27_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAY8665803.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPDFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNZ27_003632"
     CDS             complement(2522..2842)
                     /gene="chbB"
                     /locus_tag="JNZ27_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY8665804.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNZ27_003633"
     CDS             complement(3141..3479)
                     /gene="osmE"
                     /locus_tag="JNZ27_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY8665805.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3681..4508
                     /gene="nadE"
                     /locus_tag="JNZ27_003634"
     CDS             3681..4508
                     /gene="nadE"
                     /locus_tag="JNZ27_003634"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY8665806.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4738..5625
                     /gene="cho"
                     /locus_tag="JNZ27_003635"
     CDS             4738..5625
                     /gene="cho"
                     /locus_tag="JNZ27_003635"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY8665807.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNZ27_003636"
     CDS             complement(5585..6160)
                     /gene="ves"
                     /locus_tag="JNZ27_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY8665808.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNZ27_003637"
     CDS             complement(6363..6848)
                     /gene="spy"
                     /locus_tag="JNZ27_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY8665809.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNZ27_003638"
     CDS             complement(7178..8146)
                     /gene="astE"
                     /locus_tag="JNZ27_003638"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY8665810.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2151 a         2056 c         1891 g         2097 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggttattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagt
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgacccctcc ccaaagctta
     2281 gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa
     2341 taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga
     2401 ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc
     2461 aatttaaata ttgcggtttt gatttatgaa ataactcttt gacgggaaaa tttaaaataa
     2521 attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat
     2581 cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct
     2641 ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac
     2701 ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt
     2761 ctgcctgtgc gcgcattttt gataccagta aagagataga catgcccgca gaacaaaaca
     2821 gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca
     2881 ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt
     2941 tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata
     3001 aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg
     3061 aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact
     3121 caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag
     3181 gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct
     3241 gctttaccat cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg
     3301 ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt
     3361 ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg tacgatcata agctgtacaa
     3421 cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata
     3481 ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca
     3541 agcccgtgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat
     3601 tcagcccgct gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct
     3661 tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa
     3721 aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc
     3781 tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca
     3841 cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actggcaacg
     3901 aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt
     3961 gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg
     4021 cggtattggc tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc
     4081 gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca
     4141 gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat
     4201 atggtgacgg cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac
     4261 agttactgac ggcattaggt tgcccggaac acctttataa gaaagcccca actgccgatc
     4321 ttgaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata
     4381 tcgacgacta tctggaaggg aaaaacctac ctgaacaggt cgccagaaca atagagaact
     4441 ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa cgttttcgat gatttctgga
     4501 aaaagtaata atttgcacct attggattgt gcgaaaaaga gtaatttgtt cacgccggat
     4561 gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatacat tgcaggagtc
     4621 gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatca acccgcctct
     4681 tttttaatcg cctcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg
     4741 gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc
     4801 gaacatttac attcattcct taatgactta cccacccgac caggggtgta tctgtttcat
     4861 ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg
     4921 ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc
     4981 tggatctgca ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa
     5041 caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta
     5101 aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac
     5161 ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat
     5221 gaacaaaaac tttgttatgg cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt
     5281 tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa
     5341 catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa
     5401 ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac
     5461 tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc
     5521 gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat
     5581 gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca
     5641 gtttaccttg cggttgtaaa agccacaggg tgtgcctgcc atcgaaccag cacgcgccct
     5701 ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacaccaccc
     5761 cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga
     5821 catccaggcg cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt
     5881 ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc
     5941 ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc
     6001 cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa
     6061 aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc
     6121 acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct
     6181 tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt
     6241 agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg
     6301 tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa
     6361 gattattcag cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc
     6421 tcaaaattag cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc
     6481 tgggtttcca tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc
     6541 gcttcagctt ttgctttatc gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt
     6601 tcttccagcg gcggacgttt catctggtca cgctggcctt tcatgatttc gtggatctgc
     6661 tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac
     6721 ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg
     6781 gcatgggcca ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat
     6841 ttacgcatat tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc
     6901 cgtgttgacg agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac
     6961 aatgaaagtg taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatggg
     7021 gagtgatgag agaatattac aacacgatga ttttgcagag attatgaaga actataccgg
     7081 atgactggtg ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc
     7141 aacttaatac ccgcagaatg atttctgcgg gtaagtatta gcttattttt tcgagcatta
     7201 atcccgcgcg taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat
     7261 gggttacggt aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca
     7321 taaaattcag cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt
     7381 gcgaaaccac ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata
     7441 gcagcgcagc aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg
     7501 gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc aaaatgtctg acgctgaaat
     7561 gggtaaacgt accaccaggc tcctgatgga acaccagcgc ctccagcccc gccgcaccca
     7621 gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg
     7681 gatgcaagga gccacgaatt gcggtatgta gatcaaggtg ccaacgcaca gattctttgc
     7741 cctggtcata aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc
     7801 tttcagcaaa tagctgccaa cgaccgccaa acattcggtt catatcgcta tggcaataac
     7861 gtttcccttg cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg
     7921 ggatctcgct gtgagatatc gcgccaagca acgcgtccag catttccaca ggtgccgtct
     7981 cattaccgtg gatccccgca gaaatcacca gcgcgccttg cggtggcgtt aatggcgtca
     8041 gttccagcac accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga
     8101 taaccggttt tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg
     8161 cgctggaacg gataaaccga accgagattc agtaa
//
LOCUS       DADMFL010000153         8158 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.156, whole genome shotgun sequence.
ACCESSION   DADMFL010000153 DADMFL010000000
VERSION     DADMFL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.156"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(61..600)
                     /locus_tag="JNZ27_003639"
     CDS             complement(61..600)
                     /locus_tag="JNZ27_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8665811.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JNZ27_003640"
     CDS             complement(665..1213)
                     /locus_tag="JNZ27_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8665812.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNZ27_003641"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNZ27_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY8665813.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNZ27_003642"
                     /pseudo
     CDS             complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNZ27_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            4824..5540
                     /gene="nanC"
                     /locus_tag="JNZ27_003643"
     CDS             4824..5540
                     /gene="nanC"
                     /locus_tag="JNZ27_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY8665814.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5560..6666
                     /locus_tag="JNZ27_003644"
     CDS             5560..6666
                     /locus_tag="JNZ27_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY8665815.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYRTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6731..7711
                     /locus_tag="JNZ27_003645"
     CDS             6731..7711
                     /locus_tag="JNZ27_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY8665816.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2425 a         1744 c         1698 g         2291 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatag gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatgtata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     3301 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     3361 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     3421 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     3481 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     3541 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     3601 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     3661 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa
     3721 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     3781 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     3841 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     3901 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     3961 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     4021 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     4081 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     4141 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt
     4201 gtttagattc taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt
     4261 gaagaccacc gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg
     4321 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     4381 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     4441 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     4501 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     4561 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     4621 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     4681 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     4741 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     4801 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     4861 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     4921 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     4981 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     5041 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     5101 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     5161 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     5221 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     5281 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     5341 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     5401 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     5461 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     5521 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     5581 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     5641 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     5701 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     5761 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     5821 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     5881 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     5941 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     6001 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     6061 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     6121 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     6181 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     6241 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     6301 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     6361 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     6421 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagaactgat
     6481 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     6541 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     6601 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     6661 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     6721 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     6781 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     6841 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     6901 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     6961 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     7021 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     7081 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     7141 cggagccagc catgatgctt gtcgttgggg aacggatact ccgctgtacc aggatttagt
     7201 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     7261 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     7321 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     7381 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     7441 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     7501 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     7561 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     7621 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     7681 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     7741 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     7801 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     7861 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     7921 ccttttcgat tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg
     7981 gccacagcgt tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt
     8041 ctggcaaata gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat
     8101 attattgctt gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcat
//
LOCUS       DADMFL010000154         8103 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.157, whole genome shotgun sequence.
ACCESSION   DADMFL010000154 DADMFL010000000
VERSION     DADMFL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.157"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..1242)
                     /locus_tag="JNZ27_003646"
     CDS             complement(<1..1242)
                     /locus_tag="JNZ27_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY8665817.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWKRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JNZ27_003647"
     CDS             complement(1239..2117)
                     /locus_tag="JNZ27_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8665818.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISHLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3059)
                     /locus_tag="JNZ27_003648"
                     /pseudo
     CDS             complement(2128..3059)
                     /locus_tag="JNZ27_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3046..3279)
                     /locus_tag="JNZ27_003649"
     CDS             complement(3046..3279)
                     /locus_tag="JNZ27_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665819.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3276..4133)
                     /locus_tag="JNZ27_003650"
     CDS             complement(3276..4133)
                     /locus_tag="JNZ27_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8665820.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4158..4487
                     /locus_tag="JNZ27_003651"
     CDS             4158..4487
                     /locus_tag="JNZ27_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY8665821.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4477..4785
                     /locus_tag="JNZ27_003652"
     CDS             4477..4785
                     /locus_tag="JNZ27_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8665822.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4827..5003)
                     /locus_tag="JNZ27_003653"
     CDS             complement(4827..5003)
                     /locus_tag="JNZ27_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY8665823.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFSYTA
                     HLWDIRWLRHRARK"
     gene            complement(5000..5410)
                     /locus_tag="JNZ27_003654"
     CDS             complement(5000..5410)
                     /locus_tag="JNZ27_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY8665824.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5407..5685)
                     /locus_tag="JNZ27_003655"
     CDS             complement(5407..5685)
                     /locus_tag="JNZ27_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665825.1"
                     /translation="MNDSYRQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5760..>8103)
                     /locus_tag="JNZ27_003656"
     CDS             complement(5760..>8103)
                     /locus_tag="JNZ27_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HAY8665826.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1925 a         2208 c         1856 g         2114 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgctt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtg actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgaagtaatc tctgttgtat tctctgtaag atcgaaattg gttttccctt ctccgcggcg
     2641 aggggtttcc cgtgtccgcg gtgaaggctt tccctcctcc gcgaaattgg gttttacagt
     2701 ttcccgaaaa cgggtttccc catttcggga aactgattgt tttcattgat aatttcatta
     2761 aggcgctcac aatctatacg gtagaacatt ttgtgctcaa gacgcttgtt ggtttcaacc
     2821 aaaatgcctc tggacacaag atgcttacgc gctacagcct gttgttcaaa tgtaagtccg
     2881 gtttcgtgtt gtatctcttc acgcgtttta tgtacgcctt ccgctgcatg ttctttatcc
     2941 tgccagtaaa aaatctgacc aaagaaaata acagcgtgcg gacttcccat gtatttaacg
     3001 agcccagggt aataagcaac cggatgtcca aaatcgagca gaagatcaga cggacgcata
     3061 gccacctccc aggcgtttaa acatttttcc gggctgaaac gccaccagcg gataactcag
     3121 ggtatgagta cgtccctgaa cctggcagac aaccttctgg ctttcggtac tgaccaggca
     3181 aacccgcaga acgtagccgt tgctggtggt gaaccactgc cccacacggg ggcaatggtt
     3241 gtatcggtga tacaggaaat taacgatgtg gcggatcatg ggagcacctc cttgtcagaa
     3301 ccattcagcc tggaatcaac aagtgcagca ccaaaaacag catcacctac acggtcgtac
     3361 agtttgccag ccagcggaga ttcaacggcc ttaagcattg ggtaaagctg gcttgtccag
     3421 atttgatgga tttcacgcaa atgcaggtat acgcctctgg cgttttgtgc gacagatgac
     3481 atgtcagccg caccagctcc tgataagctc ctctccatct ggttaaaggc attgatgtat
     3541 gcctctttga accgggcggc acgtttacca gtgaagccca tagcaaggaa ggcgaagccg
     3601 tcgcgggtaa tttggtagca gggaagtttg cgagtaccgc cgttgggctg gcgtaccaaa
     3661 attgatgtct ccgcaaaatt gcgggcacaa aactctggag aacaatccaa aatgcggatc
     3721 tttttcagaa catcgtcatg acgtttagag aagaagttgg caacagccag ggatgaagta
     3781 acagcctgac catcaacgat ggcaatttca ggttgagaga gggttgggag agtagtcatg
     3841 gtgacagccc ctatgttgaa ttcaaagaac tcaccacatg ggacgccaat cacagaggtg
     3901 gtgagacgta cagggttggc gtaaccggtc aacatagaac ccggcgcatc ttgcgatgcc
     3961 cctgcacgcc ccaccataat ttgggcgtag caatgctcat gacacgaaaa aaccgcatga
     4021 gcgcggttat gctctatatt gaatttcagg acgccaatcc cggcacccgc tttataaggt
     4081 gcagagacag tgtaacgtcc cgaaattgca gaatcaatat attgtgcaga tataacgatt
     4141 ttgttatgtt ttgctgtatg aactacacta tcgaatacta cagtgaagag gtccgactgg
     4201 aggtcgatca gcttccattg agtatgcgtg ccagatacca gcatcttgtt gaacgcatga
     4261 aggtatacgg cagcaatctc ggagaacctc acaccagcgc ctttggtgac gggcttttcg
     4321 aactcagaat taaaggcaac gatgggatcg cgcgtgtttt ttactgcact ctgacaggga
     4381 aacgcatcat catgctgcat agttttgtaa agaaaacgca gaaaacaccg ccagccgaac
     4441 gcaagaaagc tgaaaccaga atgaaggagg ttaagcatga ctggtaaacg cactctcccc
     4501 accatgacac acgacgaaat ggcagccaaa tggatggaag acccggcttt taaagcagaa
     4561 tacgacgcta tcgctgacga attcgcactg cttgatgaaa tgctggcagc acgcaaagaa
     4621 gctggcttaa ctcaggctga agttgccgag cgaatgggaa caaaagcgac cgcgatcacc
     4681 agaatggaaa gtaatctcgc atcaggtatc agcggcccat catttgccac actaaaaaaa
     4741 ttcgcccgcg ctactggaaa aaaactccag atccgcttcg tttaaccaca ccgcgccgtc
     4801 attctggcgg cgcaccggat aaatagtcat ttcctcgcac gatgtcttag ccaccggata
     4861 tcccacaggt gagccgtgta gctgaaggtt tttacgtcag attcttttgg gattggcttg
     4921 cgtttatttc tggagcgttt cgttggaagg tatttgcagt tttcgcagat gatgtcggtg
     4981 aaacttcgtc gctgtcgcct cattcatacc tcctgtcggt aaatctgaca ccctgaccaa
     5041 tagcccaggc tgttgtgtac tcgatcagac ttgccatgcg cttcacgctc atctgcgcgc
     5101 tgctttcgcg aatgttgacg cattctcctt caaggccggg caaaacatca gcttcctgtt
     5161 ttgttgccac tgcatgaccg ctgatcaaca aaaccttcca ttgttctggt tttaaccatt
     5221 tgccgcacca ttgaacctga cgagcgatat ccgccagcat cgcgtgaaat tttgcgttct
     5281 ggtcaagatt tcgcttgtag tcagtaatgc ggatggtgac tggcttgtct ttatcgagag
     5341 gagttgagag gatggcattt attgcggctt gctgttgttg cttagttcgg aggaagattg
     5401 tttgcttcat cgaaaattct tctctttaat tccagcggct ctgatagctt tcattactgc
     5461 aattaccgtt ttgtccttcc catcctcatg ccccatcgca taagcacctt cttcaccatc
     5521 tttccaaaag tcgtcattcg attcgggcca gtcgatatcc agttcaatag ctgctcgcgg
     5581 tgcctgccat atcacccagg caaactcttt taattcatcg tctcctgtga actggctttc
     5641 gtcttttgac caccagtttt caaactgtcg gtagctatcg ttcacttccc tctcccccaa
     5701 ataaaaaggc ctgcgattac cagcaggcct gctattagct cagtgatgta gatggtcatt
     5761 taatactccg tcacgttttc ctgtcgccac gcctcgtcat attccgattt cggcatattg
     5821 gcgatgtagc tatatggcga tcctgattca agttgcagga actggtgcga ttgctcgtca
     5881 aggaacaacg ggacaccacc ttcccaacct tcgccgttac gttgtttttc aagcatcaaa
     5941 acagatgccg gagatgccag tagctgttcg tccttctctg acatcttttc accactctga
     6001 actctctgta acgctctctc gcgagccttg ttacgccaga tgatgaaaag gttgtctgtc
     6061 aggtctgtta tcgctccaga gccttttacg tccattttcc cggttggttt ttcttcgctg
     6121 tctccttttc gcgagtgagt aacgagaatg acgtgggagt ttgttttgtt tttgaagtcg
     6181 caaatcgagt caacaaacgc cttctgcccg ttatagtcat cgtcgcctat gccacatttc
     6241 atcaggctgt cgatgatgaa taactggatc ccgtatcggc ggcgagcgta gtcgaatatt
     6301 tcgatcagcc tgtcggcttt cgccgttcca gtcaggccaa acacccaaag tctttcgtca
     6361 taaaatttaa atgcagagtc aatttccagc actggcggca tcttgcagca cgttgcctga
     6421 cgggtaagtc gcttaaggag aataccaggc ttcagctcaa gtgacgcgat gcacgtcttc
     6481 acaccctgac gcattgcctc aagtgccata tgcccgacaa cctcggtgtt atgcgtcgcc
     6541 acatatcccc tggttacata caggtggcga gggtgatcga ccataataca caagcattct
     6601 gcattgccga ttttttcaac attcctgacg aataccccac agccctcgaa tcgatgcgca
     6661 gttaatctgc cattgagtcg tggtgaacgg atggcctccc tgatttctct tgtcagtctg
     6721 attcttgcct cgtatgaatc catcccatgc cgcttatcgt ccttgtatgt gtatgtcacg
     6781 ccagttttaa ctcgcgtccg gcatgacccg ccgagtgagt taaccagttg aacaacccca
     6841 ttgcgcagtt cttcacttgc tgaggaaaaa acaagcgttc catccttctc gacataccca
     6901 tccgtttcaa gcagaccaca cagcatgcct atacgcgttg atttatttgc ggaaaaaaac
     6961 acacgaggga tgaatttgtt ttttgctgtg caccccatta gtccataacc tcgcagggtc
     7021 tccatgagtg gatttacctg accacgcgcc gttgatatca gccagtcctt accatctcca
     7081 gagaagttgt aatcaggcag ttcagccttc atacgctcga tcatgtatgg ctcgacgttt
     7141 gaaaacttca cgctcccatt gctaagacta ccatccccga gaagggagcc gataacccat
     7201 gctaatggct ctgagtggtc gccaaagtca ccagttattt caggaatcct aacgccattt
     7261 ttgtgcctct tcgtttcact caaccgcttc aacccgaagg tatctatcac gcggcgtttc
     7321 tcgcctttcg tgaatccacg actagtgacc tcccataggt gatcgcctgc gcaatcaaca
     7381 taacgaccat cttcaaatgt gactcggtaa acatctctaa caccctgcgg gaatatccca
     7441 gtgacagttg acggattgcc gtctactgac gccacctgat cgccaatttt tacattcccg
     7501 tgagtagtcc atgtcccatc agctaaaaga ataggctcat tcagtgggca tgcctttccg
     7561 tgaccgttca caccattgac cagcgtcaac tctgcctcac ggaactggaa tttatctgcc
     7621 agagattccc acggtggatt aaacagatac tgctgcttgc cgtagaaagc gttgatagta
     7681 tcctggtaaa actctcgcgc actgtagagt tcttcaggat cgaagtagga tgccgtgccg
     7741 atgtactgcc agatttcatc ctcggtaaca ccgttcatca ggcattcgtt gatgtctttg
     7801 tacggcagag taacaagacg gcaacgatgt tcaccgagtc ggcttgcgat ttcccttgcg
     7861 gcttcacgac caacatcatc aacgtccatc gagatgaata tttcctcaaa cctgtcgagg
     7921 ttatgatact caaactcaat ccactgttgc ttagcgcctt tcccgccacc aaacggcacg
     7981 gataacgccg agatgccgta ttgcgcatag ctcatacaat caatttcgcc ttcgcaaagc
     8041 acaaccgccc tcacgccagc gtccagagcc tgccatccga acaggcaagg ttcgcaatca
     8101 cct
//
LOCUS       DADMFL010000155         7935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.159, whole genome shotgun sequence.
ACCESSION   DADMFL010000155 DADMFL010000000
VERSION     DADMFL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.159"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JNZ27_003657"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JNZ27_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY8665827.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGYIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="JNZ27_003658"
                     /pseudo
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="JNZ27_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="JNZ27_003659"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="JNZ27_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="JNZ27_003660"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="JNZ27_003660"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8665828.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="JNZ27_003661"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="JNZ27_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY8665829.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /locus_tag="JNZ27_003662"
     CDS             complement(5042..6469)
                     /locus_tag="JNZ27_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8665830.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GIGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="JNZ27_003663"
     CDS             complement(6492..7184)
                     /locus_tag="JNZ27_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY8665831.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            7664..>7935
                     /locus_tag="JNZ27_003664"
     rRNA            7664..>7935
                     /locus_tag="JNZ27_003664"
                     /product="16S ribosomal RNA"
BASE COUNT         2032 a         1904 c         2095 g         1904 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggat atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctgata catgctgcat tacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacggccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccgatcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt ttatggcaca ttaacggggc ttttgctgaa aaaatgcgcg gtcagaaaat
     7321 tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac
     7381 ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaattc cgtcagagaa
     7441 agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc tcgcgccgct
     7501 gagaaaaagc gaagcgcact gctctttaac aatttatcag acaatctgtg tgggcactcg
     7561 aagatacgga ttcttaacgt cgcaagacga aaagtgaata ccaagtctca agagtgaaca
     7621 cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga agagtttgat
     7681 catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga
     7741 agcagcttgc tgtttcgctg acgagtggcg gacgggtgag taatgtctgg gaaactgcct
     7801 gatggagggg gataactact ggaaacggta gctaataccg cataacgtcg caagaccaaa
     7861 gagggggacc ttcgggcctc ttgccatcgg atgtgcccag atgggattag cttgttggtg
     7921 gggtaacggc tcacc
//
LOCUS       DADMFL010000156         7910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.160, whole genome shotgun sequence.
ACCESSION   DADMFL010000156 DADMFL010000000
VERSION     DADMFL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.160"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(123..953)
                     /locus_tag="JNZ27_003665"
     CDS             complement(123..953)
                     /locus_tag="JNZ27_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY8665832.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="JNZ27_003666"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="JNZ27_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY8665833.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="JNZ27_003667"
     CDS             2379..2729
                     /locus_tag="JNZ27_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY8665834.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="JNZ27_003668"
     CDS             2855..3415
                     /locus_tag="JNZ27_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8665835.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="JNZ27_003669"
     CDS             3418..6384
                     /locus_tag="JNZ27_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY8665836.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="JNZ27_003670"
     CDS             6451..6828
                     /locus_tag="JNZ27_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8665837.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="JNZ27_003671"
     CDS             complement(7029..7688)
                     /locus_tag="JNZ27_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8665838.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2242 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DADMFL010000157         7859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.161, whole genome shotgun sequence.
ACCESSION   DADMFL010000157 DADMFL010000000
VERSION     DADMFL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.161"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            81..408
                     /locus_tag="JNZ27_003672"
                     /pseudo
     CDS             81..408
                     /locus_tag="JNZ27_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="JNZ27_003673"
     CDS             complement(438..3350)
                     /locus_tag="JNZ27_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665839.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="JNZ27_003674"
     CDS             3353..3469
                     /locus_tag="JNZ27_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8665840.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="JNZ27_003675"
     CDS             3833..4675
                     /locus_tag="JNZ27_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8665841.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="JNZ27_003676"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="JNZ27_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8665842.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="JNZ27_003677"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="JNZ27_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8665843.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="JNZ27_003678"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="JNZ27_003678"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8665844.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDECIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2115 a         1761 c         1861 g         2122 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cttggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc tgccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaat
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgtaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctgt cgtccagcaa ccggatgctc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaagcat cttcggcagg acttgttgag
     4381 cgggagagaa tgtttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacacc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattggga attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aacaaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aatcaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaatccgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gaatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DADMFL010000158         7655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.162, whole genome shotgun sequence.
ACCESSION   DADMFL010000158 DADMFL010000000
VERSION     DADMFL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.162"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..332
                     /locus_tag="JNZ27_003679"
     CDS             <1..332
                     /locus_tag="JNZ27_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8665845.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
     gene            332..679
                     /gene="tnpB"
                     /locus_tag="JNZ27_003680"
     CDS             332..679
                     /gene="tnpB"
                     /locus_tag="JNZ27_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8665846.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            699..1928
                     /locus_tag="JNZ27_003681"
                     /pseudo
     CDS             699..1928
                     /locus_tag="JNZ27_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1932..2091
                     /locus_tag="JNZ27_003682"
                     /pseudo
     CDS             1932..2091
                     /locus_tag="JNZ27_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2680..2978
                     /locus_tag="JNZ27_003683"
                     /pseudo
     CDS             2680..2978
                     /locus_tag="JNZ27_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3060..3197
                     /locus_tag="JNZ27_003684"
                     /pseudo
     CDS             3060..3197
                     /locus_tag="JNZ27_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3517..4194
                     /gene="ospD1"
                     /locus_tag="JNZ27_003685"
     CDS             3517..4194
                     /gene="ospD1"
                     /locus_tag="JNZ27_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY8665847.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            4518..4628
                     /locus_tag="JNZ27_003686"
                     /pseudo
     CDS             4518..4628
                     /locus_tag="JNZ27_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4706..5020
                     /locus_tag="JNZ27_003687"
     CDS             4706..5020
                     /locus_tag="JNZ27_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665848.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            5302..5868
                     /locus_tag="JNZ27_003688"
     CDS             5302..5868
                     /locus_tag="JNZ27_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665849.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(6073..6189)
                     /locus_tag="JNZ27_003689"
                     /pseudo
     CDS             complement(6073..6189)
                     /locus_tag="JNZ27_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6395..6949
                     /locus_tag="JNZ27_003690"
                     /pseudo
     CDS             6395..6949
                     /locus_tag="JNZ27_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            7289..7655
                     /locus_tag="JNZ27_003691"
                     /pseudo
     CDS             7289..7655
                     /locus_tag="JNZ27_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2228 a         1677 c         1788 g         1962 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag cagataaata caccgtccga cagtctgtgc tgtgagctgg
      241 ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta ttacagatac
      301 ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag gttcgcgtat
      361 ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg cctcaaaagt
      421 tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc gcggacgccg
      481 gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct ttaccagacg
      541 cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc accttactcc
      601 ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac gaacggaacg
      661 cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca ctttctcatg
      721 agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggcga
      781 aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc aaccggctga
      841 aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa aaacttcgcg
      901 caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt caggaagaaa
      961 tggcggaaac gctgggtgag caatatgacc cggtactgcc atctcccctg cgccagtctt
     1021 cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc cgtccggaag
     1081 aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat gtgtcagagc
     1141 agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg aaactggcct
     1201 gttgccggtg tgaccatatc gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt
     1261 atgccggagc ggggcttctg gcccatgttg tcgctggaaa atatgcagac tatctgccgt
     1321 tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt gccacactgg
     1381 ggcgctggac aggtgctgtt gctgaactgc tggaaccgct gtatgacatc ctgcgccagt
     1441 atgtgctgat gcccggtaaa gtccatgccg atgatatccc cgttccggtc caggagccgg
     1501 gcagcggtaa aacaaggact gcccggctgt gggtctacgt tcgtgatgac cgtaacgccg
     1561 gttcggaaat gcctccggcg gtctggttcg cgtactcacc ggaccggaaa ggtatccatc
     1621 cacagaatca tctggccggt tacagcggag tgcttcaggc cgatgcttac ggtggttacc
     1681 gggcgttgta cgaatccggc agaataacgg aagccgcatg tatggctcat gtccggagaa
     1741 aaatccacga tgtgcatgca agagtgccaa ccgacatcac cacggaagcc ctgcagcgta
     1801 tcggtgaact gtatgccatc gaggcagaag tccggggatg ttcagcagaa cagcgtctgg
     1861 cggcaagaaa agccagagcc gcgccactga tgcagtcact gtatgactgg atacagcaac
     1921 agatgaaaat tcattcgctg aaaatggaat gtctccacgg agaacactat tatccatcgg
     1981 gaaatagtgc gggcaacagt gtttagttat atcgagtgcg attacaatcg ctggcgacgg
     2041 caccgttggt gtggtggtct cagtccggaa caatttgaaa accagagcct cacttacgac
     2101 tgtgtccata ttatgtgggt aggatcaata aagacgtttt atatgtgtct gcttgcagcc
     2161 agaaaattta taccctgcag gtatcataaa catgcttgac aacttaatta gggccccctc
     2221 ttccttttat ggtgtaattg tttgattgaa tacttccggg gatttcagta tgaaatgaag
     2281 tatatttaat atactttaca aaaagtttgt attagtggaa taagaacaat agtttatcca
     2341 aactgccaag cccctttatt ataagtaaga tctggcactg gaatattctc aaaaggacta
     2401 acatcatcac cacgaccttc aaccaaccat aaaaaacaat actattttag gtgatgtatt
     2461 aaattagtag aatatttatt cttctataaa agcactaccc caagaggtta atcaggtcat
     2521 ccatgattat ttatcatata ctaactatgt gggatttcaa cgtgaaaact tattttcact
     2581 ataatcttca ggattcgaag atcataggag cctgtacata agtttgtata attctctgat
     2641 gttgaactat ttaatttgat atcaaatgaa ggtgaattta tgaatgaaaa acagttgaca
     2701 tctaataagt tgcgggcttt agctaatgaa ctagccaaaa gtctcaaaaa tcctgaagat
     2761 ctcagtcagt ttgattggat gctgaaaatg aaaccgtact caatgctaat atgactcatc
     2821 atcttgggtg cgagaaaaat cagcttagat ctggcagcaa ttcccgcaat ggttgcttaa
     2881 cgaagattat cactactgga gatgaacctc tggaaatccg tactctgcgt gatcgtaatg
     2941 gcactttcga accgcaacag ctgaagaaaa accagcccta gattactaaa tctgtcgggc
     3001 gatggattaa ctggttcagg ctgtctggtg ctgaaggcct gaagtcactg ctgtcagtga
     3061 tgtggccggc tcttcatgag acaataaccc gtaatcatca gtgccgttca atatggccac
     3121 tgttgaaaaa agttcgtcac tttatggaga cggttagccc acttcccgga gaaaaacata
     3181 gtctggacaa agtgtagcgg tattaggctc acatatttag atgtagataa gccggagctg
     3241 gcagttaaat ctaggcagca tcacagaaat ggacaacgcc ccttaaggac tggcgtatgg
     3301 caatgagctg ctttattatc aagtttggtg atcgccttga cggtcacttc tgagtgaagg
     3361 cgcttgcaca gaatggtaaa cggactcgat tattgaatct tagggataag atatatctac
     3421 gatatgtcaa tatacgaaat cgaaagaatt tattggtgaa tatagccatt caatgtggca
     3481 atggtttttt tagattagca aaaaggatga acaaaaatgt caataaataa ctatggatta
     3541 catccagcaa acaacaaaaa tatgcaccta ataataggca gcaatactgc taatgaaaat
     3601 aaaggaatga aaaataatat cattaacgtg acaaataccg ctatatccca cgccatcaat
     3661 gaagaaaaat cagggggggg atatagtggt gtttctttca gaaaattggc caaaatacag
     3721 agcatatcca ttccgacaaa gaataataag gagtataacc gccataattt gttttcattg
     3781 atttggcatg gaaatgccga tgcagcgcgt aaatacggtg aatcgctgtt ggcagccgaa
     3841 atacccaaag aggaaaaact agaagttctt gcagcacgaa ataatgctgg ggaatctgct
     3901 ttgttcatag ctcttcaaga aggtcattcc gctgcgattc aagcttatgg agattttatt
     3961 aaaacttttg atttatcacc aaaagaaacg attaaactat tggatgtaag agataatgag
     4021 gggttaccag gattatttct ggccgcaggg aaagggaata tcgaggctat gatggcatat
     4081 ataaatatat gccatcatag tgggataaaa cttacagaaa tagcagacag acttaacaat
     4141 aatgaacaag acatgtttaa tattatttct gacaaaatac aagagttgtt ttaagtgtgc
     4201 taaatagctg caaagaattg cacttagaag gatctctccc cgaattgtgt gatctgctta
     4261 atttctaaaa agaaacaaac agttaactag actgagcgat actacccata gaaccagttt
     4321 cccatgacga acgacactgg acgcaaaaac aatcataaaa aacacaataa atgtaatgaa
     4381 cacatcccac cctattaagt aaagtgcact gtgatagagg aagggaaata caggaaggtg
     4441 tggagccttg cagatttctg gcatcatagt gcccaatacg gaatgcataa agccccctca
     4501 gactggagac tccacaaatg aaatatacag tgtttaatta tatcaagtgc gattacaatc
     4561 gctgacgacg gcacagttgg tgtggaggtc tcagtcctga acaatttgaa aaccagaacc
     4621 tcacttaagc ctgtgttcat attacgtggg taggatcata cttttataga agctgtcagc
     4681 ggctacgtcc cagccaaatc acttcatgct ccagcgccag agaggcaagg ttgggtttgc
     4741 tcagctgccg gttgattttg ttgcgattga accggacagc gtccagggcg tcggcaaaag
     4801 agcgaatctc accaatcgtt gtttcattat ccgcattaac gatagcttca agaaacgcca
     4861 aggttttatc gaattcatca gcaacagtgg ctccgggcat accgtgaccg tttatacaaa
     4921 acggcgtttt cagcgtggag ttttcatgaa tagcctcaac acgaatgtcg tgaagccagt
     4981 gatgtatcgg ctacgatcct cagctgatgc cagaccatag gactgacact acgaacagca
     5041 attttaatga cgtaaatctt cataactaca actaacaggg catcattatg aatgatgccg
     5101 gttattttac cgctgttggt cccttcattt agggactgcc ccaagttttt tcatactctc
     5161 tttctcagag agtttagtct ctgggattcc agacggtcca tcattgagcc aagggagtaa
     5221 agaactcaaa gcatttacag aattgtttga atcagctaaa ataatgacaa tgttactatt
     5281 ttacatgaca aagtgattat aatgcttgga acatctttta ataattttgg aatcagttta
     5341 tctcacaaaa gatatttttc aggaaaggtg gatgagatta tccgctgtac aatggggaaa
     5401 aggatagtaa aaatatcatc aaccaaaata aatactagta ttttgagctc tgtttcagaa
     5461 caaatcggtg aaaatattac agattggaaa aatgatgaaa aaaaagtcta cgtatcccgg
     5521 gtggtgaacc aatgtattga taagttttgt gcagaacaca gccgaaaaat cggcgataat
     5581 ctccgtaaac agatatttaa acaggttgag aaagattatc gcatttctct agacatcaat
     5641 gctgcacaaa gttccataaa tcatcttgta tcaggaagca gttatttcaa aaagaaaatg
     5701 gacgaacttt gtgaagggat gaatagaagc gttaaaaatg atacaacaag caatgttgca
     5761 aatcttattt ccgatcagtt ttttgagaaa aatgtgcaat atattgatct aaagaaatta
     5821 cgtgggaata tgagtgacta tattacaaat ttagaatcgc ctttctgatg aaagaacatc
     5881 ccgagtaaag aaactaagaa aagtaaatac ccaggaattg cctgagcggt gctatatttc
     5941 tcctcgcaag taagtcctaa tatattgaat tgttgtatag ccagtttttg tattttccta
     6001 gctcatggat gtgggatccc tctccccttt ctctgctcaa aggtaagcgg ctcgtcagaa
     6061 tcatattgat atttactgag aagtcagatc aactttccaa ggcaacagat cgcgtacccg
     6121 gtttgccggt cagtcctgga tatgttcaat aacgtaacgc agccattttt ctggctccac
     6181 attgttcaga cggcatgtgc cgatcagcga gaacaacacc gctgcatgtt cgtcaccgct
     6241 gtcggaacct gcgagtcgag tcagccggag agagtttcac tctccggctg cttacggaac
     6301 cgtacgtgac actctcgcgt catacggctc ttgatcactc attcgatgca cgtagtaaat
     6361 accagtgagc aaattgatct tacccagcaa tacggtcagt taccgcgcac atggcctgcc
     6421 tgtgtcagaa aatctgctgg agcaggattt ttacgccagt ggtccgaacc agaagtgggc
     6481 gggagacatc acgtacttac gtacagatga aggctggctg tatctggctg tggtcattga
     6541 cctgtggtca cgtgccgtta ttggctggtc catgtcacca cgcctgacgg cgcaactggc
     6601 ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagaga ccccggaacg ttatcgttca
     6661 ctcggaccga ggaagtcagt actgttcagc agattatcag gcgctgctga agtggcataa
     6721 tctgcgtggc agtatgagcg caaagggctg ctgctacgat aatgcctgcg tggaaagctt
     6781 ctttcattcg ctgaaagtgg aatgtctcca cggagaacac tttatcagcc gggaaataat
     6841 gcgggcaaca gtgtttaatt atatcgaatg tgattacaat cgctggcgac ggcacagttg
     6901 gtgtggcggc ctcagtccgg aacaatttga aaaccagaac ctcgcttagg cctgtgtcca
     6961 tattacgtgg gtaggatcac ttacgccctg tggttgaaac gtgagcatat tgttttcaga
     7021 tgctgttgtc ttgcttcaga gtgatctgtc ctccggattt acgcaaagtt atccacaggc
     7081 actgtgcatt taaaaaactt atcttttctt cttgatttta aaaactcaga tcgtcatatc
     7141 attatgataa acataataaa aaactctaaa catgagaagg tttttaacgt aaggtgagag
     7201 gatttttaag tgtatccata gattaaaaat gacaatgcct ccaattagtt gaattagggg
     7261 ttataatacc cgcttttaag gttctttcat ggtcacagtt aactttcatt gcccccgttg
     7321 ccagtccgtt caggtttacc gtcacgggca gaacccaaaa ggacatgatc gattccgttg
     7381 ccgtgactgt caccgggtgt ttcagctcac ttactgttac gaggcccgta aaccgggagt
     7441 taaaaaccaa atcactgaaa tggctttcaa cggtgccgga gttcgcgata ctgcccgtac
     7501 gctgaaaatc ggcatcaata ccgtcatacg gacattaaaa agctcccgcc ccggcggata
     7561 acatctgcgc cagtcgctca cgctgatgtt gcccttatct gtgaacttga tgggcagtgg
     7621 agcttcgttg gcagcaaggc gcaacaacac tgtca
//
LOCUS       DADMFL010000159         7498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.163, whole genome shotgun sequence.
ACCESSION   DADMFL010000159 DADMFL010000000
VERSION     DADMFL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.163"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            326..568
                     /locus_tag="JNZ27_003692"
     CDS             326..568
                     /locus_tag="JNZ27_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665850.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(898..1827)
                     /gene="fdhE"
                     /locus_tag="JNZ27_003693"
     CDS             complement(898..1827)
                     /gene="fdhE"
                     /locus_tag="JNZ27_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY8665851.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1824..2459)
                     /gene="fdoI"
                     /locus_tag="JNZ27_003694"
     CDS             complement(1824..2459)
                     /gene="fdoI"
                     /locus_tag="JNZ27_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8665852.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2456..3358)
                     /gene="fdxH"
                     /locus_tag="JNZ27_003695"
     CDS             complement(2456..3358)
                     /gene="fdxH"
                     /locus_tag="JNZ27_003695"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8665853.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3371..6421)
                     /gene="fdnG"
                     /locus_tag="JNZ27_003696"
     CDS             complement(3371..6421)
                     /gene="fdnG"
                     /locus_tag="JNZ27_003696"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5834..5836),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8665854.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6615..7448
                     /gene="fdhD"
                     /locus_tag="JNZ27_003697"
     CDS             6615..7448
                     /gene="fdhD"
                     /locus_tag="JNZ27_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY8665855.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1710 a         1955 c         2015 g         1818 t
ORIGIN      
        1 gagcaatatc tggagcggca gcaatttccg gtgcgtaaag caaagggagg caggcaaaag
       61 ggggaggtag ttggtgttga tgatcagtgt aaggagcata aggaatgaaa aaaatttttc
      121 attcttgaat ataaaaaaca gatgccctta ttctggtatt aatacaaggc tgttttactt
      181 gaacttataa taactgcaac tgttacatcg tatctggaaa acgcctcgca aaaataaaaa
      241 atgatgcgta aatgagcttt ctttaaagat tcggcaaggg tacttttctc tccatagatt
      301 caaaaatgat attggcgaga tatttatgaa ctctctggca ggtatagata tgggcagaat
      361 tttactcgat ttatcgaatg aggtgattaa gcaacttgat gatctcgagg tgcagcgtaa
      421 tcttcctcgt gcggacctat taagggaagc ggtagatcaa tacctgataa atcaatcgca
      481 aacagcaaga accagtgttc ctggcatctg gcaagggtgt gaggaagatg gtgtcgaata
      541 tcagcgtaag ctgcgcgagg aatggtaatg ggaaaaaatg atgtcaatca gattgctgac
      601 aacgtgcgcg ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaagtcat
      661 gcaaattcaa tatattgaag tttattggta ggcctgataa gcgcagcgca tcaggcaatc
      721 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
      781 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      841 gtcacaccct atgagctgta atccgggcga cctgcgccac ccggaaaacg tcagagatta
      901 ctctccttca cccggaaaca ggaacgggtt aatggaacta cgggcgtagc cttcttgctc
      961 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
     1021 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
     1081 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
     1141 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
     1201 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagagccac ataccgggca
     1261 atattgacgt tgttcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
     1321 cgcccagtag agcgacagcg cagcccagat aaacggcgct ttatcgctgc tgacggacga
     1381 gaaatcagag gcaaacagcg cgctggccat atcttccagc tcctgagtcg atgccttctc
     1441 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaattag
     1501 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
     1561 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
     1621 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
     1681 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1741 ccgagggaac aataacggcg gaatcatatt cgccgtacgt ttctcgctcg aacccagctc
     1801 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1861 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1921 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1981 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     2041 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     2101 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     2161 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     2221 aggttttgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     2281 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     2341 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     2401 atccagtggt tgatacgttc cggcgcggtg tagcgcacga tggtgtcacg tcgtttcatt
     2461 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     2521 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     2581 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     2641 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacatgacg tgtgtaccac
     2701 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2761 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2821 ggcaggtctt cacgcaggct ggttcttgcc caacaaccac gcggtcaacg cacagcgtac
     2881 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2941 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     3001 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     3061 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     3121 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     3181 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     3241 tacaggtggt aacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     3301 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     3361 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     3421 tgttcgcatc accaacgaat ggcgttaaag tattggcaat aaagcctttt ttagctacgc
     3481 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     3541 gcgtgcgaat acgtttggtc accaccgctt tggctttgat atagccacgg ttggaggaga
     3601 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     3661 caaactgctc tggctgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaagt
     3721 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3781 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3841 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3901 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3961 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     4021 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     4081 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     4141 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     4201 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     4261 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     4321 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     4381 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     4441 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     4501 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     4561 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     4621 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagt gggtcgatgg
     4681 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4741 caacagggtt aaagccctgg cagatatagc cattgacctt gccctctttc atcatctcga
     4801 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4861 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4921 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4981 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     5041 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     5101 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     5161 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     5221 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     5281 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     5341 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     5401 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     5461 catcttcaaa gccgtaatcc tcacgcacga tcaggctggc gttggtatag gcttcggtgt
     5521 attcgtggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     5581 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     5641 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     5701 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5761 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5821 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5881 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5941 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     6001 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     6061 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     6121 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     6181 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     6241 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     6301 gcagtttata ctgccgggtt tccgcgagtg ctacgctggg tgcaaaaccc agtgccgctg
     6361 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     6421 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     6481 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     6541 tccctccatc taggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     6601 ctggttaaga gaaagtgaaa aaaacacagc gaaaagaaat cgaaaatgtg acaaatatca
     6661 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6721 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6781 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6841 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6901 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6961 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     7021 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     7081 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     7141 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     7201 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     7261 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     7321 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgc tgtaatctga
     7381 cactggtagg tttttgtaaa ccgggtcggg caacggttta tacccatccg cagcgtttga
     7441 gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat tcatttcg
//
LOCUS       DADMFL010000160         7451 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.164, whole genome shotgun sequence.
ACCESSION   DADMFL010000160 DADMFL010000000
VERSION     DADMFL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.164"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            215..291
                     /locus_tag="JNZ27_003698"
     tRNA            215..291
                     /locus_tag="JNZ27_003698"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /locus_tag="JNZ27_003699"
     CDS             complement(337..1098)
                     /locus_tag="JNZ27_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8665856.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="JNZ27_003700"
     CDS             complement(1281..2171)
                     /locus_tag="JNZ27_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY8665857.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNZ27_003701"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNZ27_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY8665858.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALDYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="JNZ27_003702"
     CDS             complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="JNZ27_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAY8665859.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1735 a         1936 c         1948 g         1832 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct tcagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggattt
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaatcaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagctg ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg caccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcg acgcggatcg ccgtgctgaa gaacctgttt
     6001 cagcacgcgc cagttagcca taaagttaat caggttgccc cagaaaagac gcaggacgga
     6061 aagcaggccc tgcgtgaggc cgtagtagcc agtgacgaaa atcactcgct gcacgatgcg
     6121 gttaaccatc aagccaaagt ttagccacag cagggtcatt aaccatgcgc tgccgctaaa
     6181 aatagaaagg aaatgccagg catcgggcca caaactttca tacgccagca acagcaaaag
     6241 ctggatcatc accagcatcg cgaggaagct gacaaagtta ctgattgccc ctttgcggtc
     6301 gcgccagaga aagtagttca gcgtcaggct ggaggtccat ttatgggttt taaagccctg
     6361 gaaaacaatg ccgatgatcc agcgagattt ttgtcgaacc gcagtcgaaa aggtatcggg
     6421 gaaatattcg cgcacgcaga tcatgtttga cgtccgtgcg tgctgtaaaa atttacgctg
     6481 ctcgcgttct ttggcttcgt ccaccaccgg aaaacggaca aaaatttccg tcataccttt
     6541 ttctttcagg cggaagccaa tgtcgtaatc ttcagtaaga ctctgcacgt cgaaagcaat
     6601 accgttaccg tcagctaaca gtgcggtcac ggcgcggcgg ctgaaacagg tgccgacgcc
     6661 tgcgctgggc acttgtccgg cgagggcttc acgcaccgga acatctttgc catgcagttc
     6721 tgaaaactca tcaatgtaag tcatgctggt gaagtgcgtc cattcgcgtt caaacggata
     6781 cactgggatc tgaatcagat ctttacgctc gaccagatag ttgaacagac gcaattccat
     6841 cggtgaaatc acatcttcgg cgtcatgcag aataaaacca gcaaaagcga aattggcgct
     6901 acgttcaaac tgggtgatgg cgtccagcac gttgttcaga cagtcggctt tgctggtggg
     6961 accaggacgc gcgcagacta ccttatgcac gttcggaaag cgagcgcaca cttcgtcaac
     7021 atcacgttga gtatcggggt cgttggggta ggtgccaaca aagatatgat agttttcgta
     7081 gtcgagcgtg gtcgccgcca gctcggccat attgccgatg acgcccgttt cattccacgc
     7141 cggaaccata atcgctaacg gtttttcatc tggtttatac agttcgcggt aactcattcg
     7201 cgggtagcgg cgataaacac tcaacttgcg tttaatgcgg cgtacccagt agacgacatc
     7261 aataaaaaaa tcgtccagcc cgctgatgaa catgatgacc gctaacgtta tcgcgattac
     7321 ttttaagccg tagagccagg tagcaaaaac atcaagaagc cagtccacac aaaaacctta
     7381 cattaacgct ggttatgttt agggtggcgt atattaaggt tttttatgaa ttgtgacagc
     7441 tttttaccat t
//
LOCUS       DADMFL010000161         7401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.165, whole genome shotgun sequence.
ACCESSION   DADMFL010000161 DADMFL010000000
VERSION     DADMFL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.165"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..233)
                     /locus_tag="JNZ27_003703"
     CDS             complement(<1..233)
                     /locus_tag="JNZ27_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665860.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JNZ27_003704"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JNZ27_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY8665861.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JNZ27_003705"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JNZ27_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY8665862.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JNZ27_003706"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JNZ27_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY8665863.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JNZ27_003707"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JNZ27_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JNZ27_003708"
     CDS             complement(3305..3424)
                     /locus_tag="JNZ27_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY8665864.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JNZ27_003709"
     CDS             complement(3385..3570)
                     /locus_tag="JNZ27_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665865.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JNZ27_003710"
     CDS             3671..3844
                     /locus_tag="JNZ27_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665866.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3906..4190
                     /locus_tag="JNZ27_003711"
     CDS             3906..4190
                     /locus_tag="JNZ27_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY8665867.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JNZ27_003712"
     CDS             4194..4529
                     /locus_tag="JNZ27_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY8665868.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JNZ27_003713"
     CDS             complement(4586..6907)
                     /locus_tag="JNZ27_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8665869.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JNZ27_003714"
     CDS             complement(7024..7233)
                     /locus_tag="JNZ27_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665870.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2032 a         1743 c         1647 g         1979 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat actgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcagc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtaggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac c
//
LOCUS       DADMFL010000162         7292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.166, whole genome shotgun sequence.
ACCESSION   DADMFL010000162 DADMFL010000000
VERSION     DADMFL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.166"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            91..432
                     /gene="yneK"
                     /locus_tag="JNZ27_003715"
                     /pseudo
     CDS             91..432
                     /gene="yneK"
                     /locus_tag="JNZ27_003715"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            462..1202
                     /gene="yneK"
                     /locus_tag="JNZ27_003716"
     CDS             462..1202
                     /gene="yneK"
                     /locus_tag="JNZ27_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY8665871.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1352..2542
                     /gene="ydeA"
                     /locus_tag="JNZ27_003717"
     CDS             1352..2542
                     /gene="ydeA"
                     /locus_tag="JNZ27_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY8665872.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JNZ27_003718"
     CDS             complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JNZ27_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY8665873.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3444..3878
                     /gene="marR"
                     /locus_tag="JNZ27_003719"
     CDS             3444..3878
                     /gene="marR"
                     /locus_tag="JNZ27_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY8665874.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3898..4281
                     /gene="marA"
                     /locus_tag="JNZ27_003720"
     CDS             3898..4281
                     /gene="marA"
                     /locus_tag="JNZ27_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY8665875.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4313..4531
                     /gene="marB"
                     /locus_tag="JNZ27_003721"
     CDS             4313..4531
                     /gene="marB"
                     /locus_tag="JNZ27_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY8665876.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4562..5461)
                     /gene="eamA"
                     /locus_tag="JNZ27_003722"
     CDS             complement(4562..5461)
                     /gene="eamA"
                     /locus_tag="JNZ27_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY8665877.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5725..6843
                     /gene="ydeE"
                     /locus_tag="JNZ27_003723"
     CDS             5725..6843
                     /gene="ydeE"
                     /locus_tag="JNZ27_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY8665878.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMPAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6970..7065
                     /gene="mgtS"
                     /locus_tag="JNZ27_003724"
     CDS             6970..7065
                     /gene="mgtS"
                     /locus_tag="JNZ27_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY8665879.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1902 a         1773 c         1664 g         1953 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
      961 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     1021 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     1081 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     1141 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     1201 aaaccgtgat gttaccgact ctctgacgcg gaacagaatc agcgtcagag aaacggaaaa
     1261 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     1321 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     1381 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     1441 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     1501 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     1561 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     1621 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     1681 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     1741 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     1801 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     1861 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     1921 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     1981 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     2041 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     2101 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     2161 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     2221 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     2281 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     2341 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     2401 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     2461 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     2521 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     2581 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     2641 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     2701 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     2761 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     2821 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     2881 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     2941 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     3001 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     3061 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     3121 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     3181 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     3241 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     3301 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     3361 gcatcggtca attcattcat ttgacttata cttgcctagg caatattatc ccctgcaact
     3421 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     3481 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     3541 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     3601 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     3661 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     3721 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     3781 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     3841 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     3901 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     3961 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     4021 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     4081 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     4141 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     4201 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     4261 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     4321 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
     4381 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
     4441 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
     4501 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
     4561 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
     4621 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
     4681 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
     4741 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
     4801 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
     4861 atgcggaacc atcgagaatc agcgaggcaa caaagaaggg aatgattggg attaaagcgc
     4921 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
     4981 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
     5041 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
     5101 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
     5161 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
     5221 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
     5281 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
     5341 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
     5401 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
     5461 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
     5521 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
     5581 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
     5641 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
     5701 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
     5761 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
     5821 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
     5881 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
     5941 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
     6001 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
     6061 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
     6121 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
     6181 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
     6241 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
     6301 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
     6361 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
     6421 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
     6481 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
     6541 aacaacctgc tattgtgggg tatgccagct gcggtattta ctgtcggtga aatcatttat
     6601 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
     6661 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
     6721 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
     6781 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
     6841 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
     6901 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
     6961 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
     7021 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
     7081 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
     7141 aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg gatagatttg tgttttgctt
     7201 ttacgctaac aggcattttc ctgcactgat aacggatcgt tgacacagta gcatcagttt
     7261 tctcaatgaa tgaggtgtac tggcaatagc gg
//
LOCUS       DADMFL010000163         7211 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.167, whole genome shotgun sequence.
ACCESSION   DADMFL010000163 DADMFL010000000
VERSION     DADMFL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.167"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..335)
                     /locus_tag="JNZ27_003725"
     CDS             complement(<1..335)
                     /locus_tag="JNZ27_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY8665880.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVS"
     gene            712..2628
                     /gene="dnaK"
                     /locus_tag="JNZ27_003726"
     CDS             712..2628
                     /gene="dnaK"
                     /locus_tag="JNZ27_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY8665881.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHTSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2717..3847
                     /gene="dnaJ"
                     /locus_tag="JNZ27_003727"
     CDS             2717..3847
                     /gene="dnaJ"
                     /locus_tag="JNZ27_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY8665882.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3951..4160)
                     /gene="mokC"
                     /locus_tag="JNZ27_003728"
     CDS             complement(3951..4160)
                     /gene="mokC"
                     /locus_tag="JNZ27_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY8665883.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            4689..5855
                     /gene="nhaA"
                     /locus_tag="JNZ27_003729"
     CDS             4689..5855
                     /gene="nhaA"
                     /locus_tag="JNZ27_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY8665884.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5921..6820
                     /gene="nhaR"
                     /locus_tag="JNZ27_003730"
     CDS             5921..6820
                     /gene="nhaR"
                     /locus_tag="JNZ27_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY8665885.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(6858..>7211)
                     /locus_tag="JNZ27_003731"
     CDS             complement(6858..>7211)
                     /locus_tag="JNZ27_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665886.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT         1819 a         1686 c         1865 g         1841 t
ORIGIN      
        1 tttgagacgc agcgtttatt gtcgttatcg ctgttaatgt tgatccagtc agtggtttgc
       61 ccttctttta tttctgaagg aatattcagg ctctgactgg cgctacgggc ggctttgaaa
      121 taaaccgatg caccgcttaa ctgtaaatcg ccatggtcgg cagagagttg tatgcgtttc
      181 acaatgcgac aaacaggaag tttcagcgcc agatcgttgg tttcgttacg cggcattgca
      241 atgacgccga ggagtttatg gtcgtttgcc tgcgccgtgc agcacagcat caggctaatc
      301 gccaggctgg cggaaatcgt aaaaacggat ttcataagga ttctcttagt gggaagaggt
      361 agggggatga atacccacta gtttactgct gataaagaga agattcaggc acgtaatctt
      421 ttctttttat tacaattttt tgatgaatgc cttggctgcg attcattctt tatatgaata
      481 aaattgctgt caattttacg tcttgtcctg ccatatcgcg aaatttctgc gcaaaagcac
      541 aaaaaatttt tgcatctccc ccttgatgac gtgggttacg accccattta gtagtcaacc
      601 gcagtgagtg agtctgcaaa aaaatgaaat tgggtagttg aaaccagacg tttcgcccct
      661 attacagact cacaaccaca tgatgaccga atatatagtg gagacgttta gatgggtaaa
      721 ataattggta tcgacctggg tactaccaac tcttgtgtag cgattatgga tggcaccatt
      781 cctcgcgtgc tggagaacgc cgaaggcgat cgcaccacgc cttctatcat tgcctatacc
      841 caggatggtg aaactctggt tggtcagccg gctaaacgtc aggcagtgac gaacccgcaa
      901 aacaccctgt ttgcgattaa acgcctgatt ggccgccgct tccaggacga agaagtacag
      961 cgtgatgttt ccatcatgcc gttcaaaatt attgctgctg ataacggcga cgcatgggtc
     1021 gaagttaaag gccagaaaat ggcaccgccg cagatttctg ctgaagtgct gaaaaaaatg
     1081 aagaaaaccg ctgaagatta cctgggtgaa ccggtaactg aagctgttat caccgtaccg
     1141 gcatacttta acgatgctca gcgtcaggca accaaagacg caggccgtat cgctggtctg
     1201 gaagtaaaac gtatcatcaa cgaaccgacc gcagctgcgc tggcttacgg tctggacaaa
     1261 ggcactggca accgtactat cgcggtttat gacttggggg gtggtacttt cgatatttct
     1321 attatcgaaa tcgacgaagt tgacggcgaa aaaaccttcg aagttctggc aaccaacggt
     1381 gatacccacc tgggtggtga agacttcgac agccgtctga tcaactatct ggttgaagaa
     1441 ttcaagaaag atcagggcat tgacctgcgc aacgatccgc tggcaatgca gcgcctgaaa
     1501 gaagcggcag aaaaagcgaa aatcgaactg tcttccgctc agcagaccga cgttaacctg
     1561 ccatacatca ctgcagacgc gaccggtccg aaacacatga acatcaaagt gactcgtgcg
     1621 aaactggaaa gcctggttga agatctggta aaccgttcca ttgagccgct gaaagttgca
     1681 ctgcaggatg ctggcctgtc cgtatctgat atcgacgacg ttatcctcgt tggtggtcag
     1741 actcgtatgc caatggttca gaagaaagtt gctgagttct ttggtaaaga gccgcgtaaa
     1801 gacgttaacc cggacgaagc tgtagcaatc ggtgctgctg ttcagggtgg tgttctgact
     1861 ggtgacgtaa aagacgtact gctgctggac gttaccccgc tgtctctggg tatcgaaacc
     1921 atgggcggtg tgatgacgac gctgatcgcg aaaaacacca ctatcccgac caagcacagc
     1981 caggtgttct ctaccgctga agacaaccag tctgcggtaa ccatccatgt gctgcagggt
     2041 gaacgtaaac gtgcggctga taacaaatct ctgggtcagt tcaacctaga tggtatcaac
     2101 ccggcaccgc gcggcatgcc gcagatcgaa gttaccttcg atatcgatgc tgacggtatc
     2161 ctgcacgttt ccgcgaaaga taaaaacagc ggtaaagagc agaagatcac catcaaggct
     2221 tcttctggtc tgaacgaaga tgaaatccag aaaatggtac gcgacgcaga agctaacgcc
     2281 gaagctgacc gtaagtttga agagctggta cagactcgca accagggcga ccatctgctg
     2341 cacacctccc gtaagcaggt tgaagaagca ggcgacaaac tgccggctga cgacaaaact
     2401 gctatcgagt ctgcgctgac tgcactggaa actgctctga aaggtgaaga caaagccgct
     2461 atcgaagcga aaatgcagga actggcacag gtttcccaga aactgatgga aatcgcccag
     2521 cagcaacatg cccagcagca gactgccggt gctgatgctt ctgcaaacaa cgcgaaagat
     2581 gacgatgttg tcgacgctga atttgaagaa gtcaaagaca aaaaataatc gccctataaa
     2641 cgggtaatta tactgacacg ggcgaagggg aatttcctct ccgcccgtgc attcatctag
     2701 gggcaattta aaaaagatgg ctaagcaaga ttattacgag attttaggcg tttccaaaac
     2761 agcggaagag cgtgaaatca gaaaggccta caaacgcctg gccatgaaat accacccgga
     2821 ccgtaaccag ggtgacaaag aggccgaggc gaaatttaaa gagatcaagg aagcttatga
     2881 agttctgacc gactcgcaaa aacgtgcggc atacgatcag tatggtcatg ctgcgtttga
     2941 gcaaggaggc atgggcggcg gtggttttgg cggcggcgca gacttcagcg atatttttgg
     3001 tgacgttttc ggcgatattt ttggcggcgg acgtggtcgt caacgtgcgg cgcgcggtgc
     3061 tgatttacgc tataacatgg agctcaccct cgaagaagct gtacgtggcg tgaccaaaga
     3121 aatccgcatt ccgactctgg aagagtgtga cgtttgccac ggtagcggtg caaaaccagg
     3181 tacacagccg cagacctgtc cgacctgtca tggttctggt caggtgcaga tgcgccaggg
     3241 attcttcgct gtacagcaga cctgtccaca ctgtcagggc cgcggtacgc tgatcaaaga
     3301 tccgtgcaac aaatgtcatg gtcatggtcg tgttgagcgc agcaaaacgc tgtccgttaa
     3361 aatcccggca ggggtggaca ctggagaccg catccgtctt gcgggcgaag gtgaagcggg
     3421 cgagcatggc gcaccggcag gcgatctgta cgttcaggtt caggttaaac agcacccgat
     3481 tttcgagcgt gaaggcaaca acctgtattg cgaagtcccg atcaacttcg ctatggcggc
     3541 gctgggtggc gaaatcgaag taccgaccct tgatggtcgc gtcaaactga aagtgcctgg
     3601 cgaaacccag accggtaagc tattccgtat gcgcggtaaa ggcgtcaagt ctgtccgcgg
     3661 tggtgcacag ggtgatttgc tgtgccgcgt tgtcgtcgaa acaccggtag gcctgaacga
     3721 aaagcagaaa cagctgctgc aagagctgca agaaagcttc ggtggcccaa ccggcgagca
     3781 caacagcccg cgctcaaaga gcttctttga tggtgtgaag aagttttttg acgacctgac
     3841 ccgctaacct ccccaaaagc ctgcccgtgg gcaggcctgg gtaaaaatag ggtgcgttga
     3901 agatatgcga gcacctgtaa agtggcgggg atcactcccc gccgttgctc ttactcggat
     3961 tcgtaagccg tgaaaacagc aacctccgtc tggccagttc ggatgtgaac ctcacagagg
     4021 tcttttctcg tcaccagcgc cgccactacg gcggtgatac agatgacgat cagggcgaca
     4081 atcatcgcct tatgctgctt cattgctctc ttctccttga ccttacggtc agtaagaggc
     4141 actctacatg tgttcagcat ataggaggcc tcgggttgat ggtaaaatat cactcggggc
     4201 ttttctctat ctgccgttca gctaatgcct gagacagaca gcctcaagca cccgccgcta
     4261 ttatatcgct ctctttaacc cattttgttt tatcgattct aatcctgaag acgcctagca
     4321 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tcttcatcgc
     4381 aattattgac gacaagctgg attatttttg aaatattggc ctaacaagca tcgccgactg
     4441 acaacaaatt aattattact tttcctaatt aatccctcag gaatcctcac cttaagctat
     4501 gattatctgg gcttagggtc actcgtgagc gcttacagcc gtcaaaaacg catctcaccg
     4561 ctgatggcgc aaattcttca atagctcgta aaaaacgaat tattcctaca ctataatctg
     4621 attttaacga tgattcgtgc ggggtaaaat agtaaaaacg atctattcac ctgaaagaga
     4681 aataaaaagt gaaacatctg catcgattct ttagcagtga tgcctcggga ggcattattc
     4741 ttatcattgc cgctatcctg gcgatgatta tggccaacag cggcgcaacc agtggatggt
     4801 atcacgactt tctggagacg ccggttcagc tccgggttgg ttcactcgaa atcaacaaaa
     4861 acatgctgtt atggataaat gacgcgctga tggcggtatt tttcctgtta gtcggtctgg
     4921 aagttaaacg tgaactgatg caaggatcgc tagccagctt acgccaggcc gcatttccag
     4981 ttatcgccgc tattggtggg atgattgtgc cggcattact ctatctgcct tttaactatg
     5041 ccgatccgat tacccgcgaa gggtgggcga tcccggcggc tactgacatt gcttttgcac
     5101 ttggtgtact ggcgctgttg ggaagtcgtg ttccgttagc gctgaagatc tttttgatgg
     5161 ctctggctat tatcgacgat cttggggcca tcattatcat cgcattgttc tacactaatg
     5221 acttatcgat ggcctctctt ggcgtcgcgg ctgtagcaat tgcggtactc gcggtattga
     5281 atctgtgtgg tgtacgccgc acgggcgtct atattcttgt tggcgtggtg ttgtggactg
     5341 cggtgttgaa atcgggggtt cacgcaactc tggcgggggt aattgtcggc ttctttattc
     5401 ctttgaaaga gaagcatggg cgttctccag cgaagcgact ggagcatgtg ttgcacccgt
     5461 gggtggcgta tctgattttg ccgctgtttg cattttctaa tgctggcgtt tcactgcaag
     5521 gcgtcacgct ggatggcttg acctccattc tgccattggg gatcatcgct ggcttgctga
     5581 ttggcaaacc gctggggatt agtctgttct gctggttggc gctgcgtttg aaactggcgc
     5641 atctgcctga gggaacgact tatcagcaaa ttatggtggt ggggatcctg tgcggtatcg
     5701 gttttactat gtctatcttt attgccagcc tggcctttgg tagcgtagat ccagaactga
     5761 ttaactgggc gaaactcggt atcctggtcg gttctatctc ttcggcggta attggataca
     5821 gctggttacg cgttcgtttg cgtccatcag tttgacagga cggtttaccg gggagccata
     5881 aacggctccc ttttcattgt tatcagggag agaaatgagc atgtctcata tcaattacaa
     5941 ccacttgtat tacttctggc atgtctataa agaaggttcc gtggttggcg cagcggaggc
     6001 gctttattta actccacaaa ccattaccgg acagattcga gcgctggaag agcgcctgca
     6061 aggcaaatta tttaaacgca agggacgtgg tctcgaaccc agcgagctgg gagaactggt
     6121 ctatcgctat gccgataaaa tgttcacctt aagccaggaa atgctggata ttgtgaacta
     6181 tcgcaaagaa tccaatttat tgtttgacgt tggcgtggct gatgcacttt ccaaacgcct
     6241 ggtcagtagc gtacttaacg ccgcagtggt agaaggcgag cccattcatc ttcgctgctt
     6301 cgaatccacc cacgaaatgc tgctggagca attaagtcag cataaactgg atatgatcat
     6361 ttctgactgt ccgatagact ctacgcagca ggaaggcctg ttctccgtga gaattggcga
     6421 atgtggcgtg agtttctggt gtacaaatcc accaccagaa aaaccgttcc cggcttgtct
     6481 ggaagaacgg cgacttttga ttcctgggcg acgttcaatg ttagggcgca aattgcttaa
     6541 ctggtttaac tcccagggat taaacgtaga aatcctcggc gagtttgatg atgccgcttt
     6601 gatgaaagct tttggtgcga tgcacaatgc aatcttcgtt gccccaacgc tttatgcata
     6661 tgatttttat gccgataaaa ctgtcgtaga aattggtcgc gtcgagaatg tgatggaaga
     6721 gtaccatgct atttttgctg agcggatgat tcagcacccg gcggtacagc gaatctgcaa
     6781 tacggattat tctgcgcttt ttagtccagc ggtgcgttaa tcggcagctc ccccaaagtt
     6841 aaggtggggg agatagatta gttgtacatt accacgattt tgactcggct cattatttgc
     6901 ccgcttgaga cattgtttcc atatgtacgc gggcgaataa atagaggaat ctgattactt
     6961 ccttcatggg gatgctgaaa agagtagtaa ttgcttttac catactggat ttcattcgta
     7021 ccatcattgg agagttgaaa gcctatttga cccgcaccgt cctcactgtt ttctaactca
     7081 ttttttaata tgctggttcc tgactcctgt atcgtcactg gttcaaagta atattcaacg
     7141 tcactgggtt caaaaactaa ttcaccttgc tcgccctgga tgcagtcaaa tcttatatga
     7201 tgaaactgat a
//
LOCUS       DADMFL010000164         7066 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.168, whole genome shotgun sequence.
ACCESSION   DADMFL010000164 DADMFL010000000
VERSION     DADMFL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.168"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..767
                     /locus_tag="JNZ27_003732"
     CDS             <1..767
                     /locus_tag="JNZ27_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8665887.1"
                     /translation="LESCEWLQYLNLSHNQLTDIVAGNKNELLLLDLSHNKLTSLHNV
                     LFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINLDFLTYLPSIKSLRLD
                     NNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNT
                     GLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTL
                     DEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            1091..2479
                     /locus_tag="JNZ27_003733"
                     /pseudo
     CDS             1091..2479
                     /locus_tag="JNZ27_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            2561..6194
                     /locus_tag="JNZ27_003734"
                     /pseudo
     CDS             2561..6194
                     /locus_tag="JNZ27_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            6191..>7066
                     /gene="narH"
                     /locus_tag="JNZ27_003735"
     CDS             6191..>7066
                     /gene="narH"
                     /locus_tag="JNZ27_003735"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8665888.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVYSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         1769 a         1812 c         1745 g         1740 t
ORIGIN      
        1 aattagaatc ttgcgaatgg ctgcaatacc tgaatctcag ccataatcaa ttaactgata
       61 ttgttgcagg taataaaaat gaactcttac tgctggatct atcccataat aaactaacaa
      121 gtttacacaa tgtcttattt cccaacttga atacgttact tattaacaac aatttgcttt
      181 ctgaaattaa aatattctat agcaacttcc gcaatgttca gacattaaac gctgctaaca
      241 accagttgaa atatataaat cttgatttcc tgacttatct tccatctatc aaaagtttaa
      301 gactggacaa taataaaata acccacattg atactaataa tacatccgat attggaactt
      361 tattccccat aataaaacag agcaaaaact taaatttttt aaatgtttct gggaagaaca
      421 attgccctac tatgcagctc atgttattta atttgttttc cccagcactt aagcttaata
      481 ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga atagatgtgg
      541 ataacgaatt gtttcactat actattaaaa aagcatatac cccatataat atacacactt
      601 acaagacaga ggaagttgta aaccagagga atataaaagt taaaaacatg accttagatg
      661 aaataaacaa tacttactat aataacgatt attacaatca ggcaataaga gaggaaccga
      721 tagactttct ggacagatcg ttttcctcca attcatggcc tttttagtga ataaatcatc
      781 actaattcta taagtgtctg gtcttgttga gaatatagtt aatatgtaac cctttagatt
      841 cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt ttgaaggtta
      901 gcttgttagt tacatttagt aacacatatc tggctactta atccccattc tgcccccgcg
      961 gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc ttaattctaa
     1021 taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat gtgtgcatgt
     1081 gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac gcgactggaa
     1141 accagaaaac ccggccttct gggaaaataa aggaaaacat attgctcgaa gaaacctctg
     1201 gatatcagtc agttgtctac ttcttgcctt ctgtgtctag atgctattta gcgcagttac
     1261 cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt tattaaccgc
     1321 attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg tgcctatatt
     1381 cggtggacgc caatggccgg tttttagtac tgcaatcctg attattcctt gcgtctggct
     1441 cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta tcgctttgct
     1501 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
     1561 aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact taggtgtaag
     1621 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
     1681 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
     1741 ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag
     1801 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
     1861 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
     1921 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
     1981 tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg
     2041 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
     2101 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
     2161 gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
     2221 aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga gagaagccgt
     2281 caccgaaacg gcggcagcgc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
     2341 tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
     2401 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
     2461 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag
     2521 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     2581 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     2641 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     2701 ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     2761 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     2821 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     2881 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa
     2941 acaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct
     3001 gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc aggaactaaa
     3061 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     3121 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     3181 gtcgctgctt ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc
     3241 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     3301 cagctacatc atcgcttggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     3361 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     3421 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     3481 gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     3541 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     3601 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     3661 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     3721 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     3781 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     3841 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     3901 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     3961 ctgtccagtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     4021 ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     4081 tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg aatttgccga
     4141 taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg ttaaccactg
     4201 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     4261 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     4321 cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag
     4381 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     4441 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     4501 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     4561 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     4621 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg
     4681 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     4741 gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     4801 cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct
     4861 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgtcctgcc
     4921 caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc catttattca
     4981 tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg aaatctacaa
     5041 aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt
     5101 ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     5161 cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga atattgtggc
     5221 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     5281 caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa tcgatttcct
     5341 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     5401 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     5461 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     5521 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     5581 ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     5641 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     5701 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     5761 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa aagcacttaa
     5821 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     5881 gctgacgctc tctcgcggtg gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc
     5941 acgcccagga acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggca
     6001 ttcataactt ggtcacccgc gtttgcccga aaccgacgca tatgattggc ggttacgcgc
     6061 agctggcctg gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca
     6121 tgatccgcaa gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg
     6181 aggcgaaaaa atgaaaatac gttcgcaagt cggcatggtg cttaacctcg ataaatgtat
     6241 cggctgccat acctgttcgg tgacctgtaa aaacgtctgg accggacgcg aaggcatgga
     6301 gtacgcatgg tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga
     6361 agatcaggaa gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg
     6421 tctgggcagc aagatgggcg taataaccaa aatcttcgcc aacccggtgg tgccgcagca
     6481 gattgatgat tactacgaac ctttcacctt cgactacgaa catttgcata gcacaccgga
     6541 aggcaaacat atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa
     6601 agtgatctgg ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg
     6661 cgaccgcaac ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat
     6721 gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg cgacctgccc
     6781 aagcggcgct atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg
     6841 ccgtggctgg cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa
     6901 aagcggcaag tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac
     6961 cgtgtactca gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc
     7021 cgaccgcatc gaggaagcgg cgagcaccga gcgcgaagtt gacctc
//
LOCUS       DADMFL010000165         7049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.169, whole genome shotgun sequence.
ACCESSION   DADMFL010000165 DADMFL010000000
VERSION     DADMFL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.169"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            147..1757
                     /locus_tag="JNZ27_003736"
     CDS             147..1757
                     /locus_tag="JNZ27_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8665889.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            1876..2209
                     /locus_tag="JNZ27_003737"
                     /pseudo
     CDS             1876..2209
                     /locus_tag="JNZ27_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708644.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3328..4056
                     /locus_tag="JNZ27_003738"
     CDS             3328..4056
                     /locus_tag="JNZ27_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY8665890.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSNCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            4354..4845
                     /locus_tag="JNZ27_003739"
     CDS             4354..4845
                     /locus_tag="JNZ27_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665891.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            4929..5918
                     /locus_tag="JNZ27_003740"
     CDS             4929..5918
                     /locus_tag="JNZ27_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665892.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            6651..6866
                     /locus_tag="JNZ27_003741"
     CDS             6651..6866
                     /locus_tag="JNZ27_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8665893.1"
                     /translation="MKSMDKISTGIAYGTAAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            6866..>7049
                     /locus_tag="JNZ27_003742"
     CDS             6866..>7049
                     /locus_tag="JNZ27_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8665894.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIM"
BASE COUNT         1819 a         1728 c         1739 g         1763 t
ORIGIN      
        1 ctgcccgctc catgacactt cttaaagtct actaacttac tgaatcctaa gggatacggc
       61 ttaacgcctt ccgatagttt tactcactgt aaccaacact gtgactaact ttgttgaagt
      121 agtgagcaga cctaaggtct cagtacatgc tcaagtcaag aacctacctt tatcagcgta
      181 atggtgtctt ttacatccgt cttaggatga agaccactag tcgcctgacc gcttcgcttc
      241 cttctcacaa ccgttataaa ctagcgtcag tatctttacg gaccaaggat agacgtaccg
      301 ctatggctca ctcacggcac atcaagtcag cacttaaagc aatccatgca gacaacccta
      361 acgcctctta tgaggagcta cgggagcacc tgaagaccat tgtcgagtgg gaacttagtg
      421 tcagccgtga tgacctgaac gacccggagt cctaccagct ttacgttgac cagtacgatg
      481 acattaagtc caaccttcgg gaagctgtag cgaccgagcg tctgactgta gaccagcatc
      541 gctatattaa tgacgttatc ggtgtgctta aggcctgtca ggatagactc aatggcgata
      601 gctctggcct attgtcttac cttgagcctg agactggcag tctaagaccg tccgtctccc
      661 tatctgtatt ggcggaacct gaagtccctg aacctaaagc actgaccctc gcgtctctca
      721 ttgagcagta cgagcaggag aacgcccaga actggaagcc agcgacccta agcgaaaacc
      781 gagcgtcaca ctctacgcta atcgagatat tcgactatct ggatattcag gatgtcggca
      841 aggcgacccg tgcagatatg ctcagagttc gtgaagtcct ccagcagcta ccgaagaacc
      901 gcaagcaacg ctttaagtct atgccgctgt ctgacttgct gaaccgggag tctaagactg
      961 actgtctgga cgtcgttacc attaacaata aatatcttat caagatggct gctgtgttta
     1021 agtgggcggt acgcaacgac ttgatagcta agaacctgac cgaaggtctt gagctgaaag
     1081 tccctcagcg taaagcctcc gacgctcgtg atgcgttcag tcctgagcag gtagggcaac
     1141 tactggtcgc agctaaagcg tactcgcaga agacctcagg taagccatac cattattacg
     1201 tcaccgctct ggctgcgatt acaggtgcga gacttaacga ggtggcccaa cttcaggtta
     1261 aagatgtcag gaccactgag gctgggaccg tttttatcca tatcaacgaa gacgatagca
     1321 gcctgccggg taagagtatc aagaacgccc acagtgaccg ctgtgtcccg ttggtagatg
     1381 gtgcttatgg ttttgtattg gctgacttta tgtcactcgt ggaagaccgt agaaagactg
     1441 agggtgataa tgctatggtg ttcaatggcc tcaagctaat gaagaacggc tacggtgagc
     1501 aggttagcaa gtggtttaac cgaacgttgc tgcccaaagt cctcgctgac cgtagcggct
     1561 tagcttttca ctcgtttaga catacagttg cgacccagtt aaaacaacat ggggtcgagt
     1621 tagcgtatgc tcaggcgatt atgggtcata gctcagggtc tataacttac gaccgttacg
     1681 cgaaagaggt cgaggtggaa acgctgaaag aaaaattagc ggagtcattg tcagtaaaaa
     1741 aaatagacgg aaagtaatcg catagggata gcggaatgtg aggacgtaat ggtcaaagaa
     1801 tttttagtta gcaaagaaaa taagccttca tatgaatgta tgaatactgc tgaaacttgt
     1861 ttggggctgg ctggaatggc ttcggaagat tggcaacgtg agcatcattg ccgcacaggt
     1921 ataatattta ctgcttttgc tattgaggca atgttcatct tctatagaaa acaggttgac
     1981 cccggctacg ataagacgca gaaagagtgc agaaagacaa tgcacaagaa tacgttaaaa
     2041 ttgtgcggta taaacaatta tatggggaca aaaccttacc aaattatcaa agagtgtctt
     2101 gaggtaagag acgctattgc tcacggcgat tcgtatactt cgagctttaa tttctcggcg
     2161 gatcatttgg ataaccaaga cgatattgca aaaagtgtcc tggcggtaaa ctctgaacaa
     2221 tttcggggat taactgttga gcgccttgca aaatacattg aaatggcaaa gcatattgac
     2281 catgagattt ctgataatgg ttacaggcct tctgaaggtt accttccagc agccgagcgc
     2341 agaagtctga tgttagcttt tggcgtcagt ggtgtgtcaa tgtggcccag tgcttgagcg
     2401 ataatacacc tcactataga ggaatggacc cttaactatc actatagggg agagacccta
     2461 agtccccacc ttaatagcag tcacgaccca tacacacatt cattaagtct caggcttccg
     2521 gtagcggacc gattggaaac catccgcaga cgcctagtgt taaatcacct tggcctgaga
     2581 ccattccttt taacgtaagc tatcttccct tttgacaggc ggggagcagt agaccacatc
     2641 cgactttaag ttggctctgt gataatcgct gcgcggataa ctgaagaacc aagacaccat
     2701 tactgaggtc tgccatccat ttgtggacag gcggaaactc aaagaggtta gatcagtggt
     2761 gtctgaggtt ggagctttga gtcttgacca gcaggctgga ggagaccatg agtttatctt
     2821 gttatggatt gtgttatcac aattccaata agaagaactt taagtccagc ctccactccc
     2881 gccaacccga agtaaaatta tggtcgctat gatgtggtct ccatgcttcg ggtcttactg
     2941 gaagtactga acactttcgc gtcaagttct ccgaagttca ttcctgtttg actaaactat
     3001 cactataggg accatatctc gcctctgacc tcctgagaga cggcaagcga gaaaccgtgg
     3061 tccacgctag caagctagcc accagcctaa ggcaagacag cctctggtca gcctgtgtgt
     3121 tctttggtct cgtgacttaa gtctatcacc acacacgctc ggacatgctg tcgaaccttc
     3181 atctctatgg tcatcgcaga ccactaaccc caattacctc aagtcattct gtttgcgtcc
     3241 tgttgcctgc tggtctccct ttacgtgggg gcttgagtag gtcagtgggg tgcgacttca
     3301 gggtgcaaac aaaaggagta cacgtaaatg tcccaacaag aaatcagcgt cattaacctc
     3361 gaccagcttg tctccatgac ctcagttgaa attgctgagc tgacaggcaa ggagcaccga
     3421 aatgtcctgc gtgatattcg caatatggct gaggagctta acgcgctcaa aactgagcag
     3481 tgctcaaaac tgagcagtca tatcggggtg actgaagatg tctatctaaa cgcgcagggg
     3541 aaacagcagc cgctgtatcg tctcgaccgt aagcatactt tcattctggt cgctggctat
     3601 tccgttcacc tccgcgccaa gtgttacgac catattcaga cgttagagcg tcgagtcctt
     3661 caactggaag accagaagaa gcgagcagcc attcagtccg ctaaccgccg aggcgtgact
     3721 tggggcgact actgcaaaac ctatggtctg cccgctcaga agctaatgac cgcacttctc
     3781 cagcatcgtg gcctctttcg taagaaccca atcagtaacg agtggtctgt aaatccgaag
     3841 tattccaact gctttcgtat cattaagccg tcagaccaga agttctcagc aggtgggtat
     3901 aacttccggt tcaacgctaa ggggctggag gtcttcggta agcctgagat ggttgacaag
     3961 atgcgaggca tccttattgc attcactggc accgaccagc agaagcaaga gcacctcctg
     4021 aagctggcac aatccggtaa ggtggaggga atctaagatg atgattgacc cgcgcacacc
     4081 tgaggggcgc atgactctcc gttaccgtgg ctaccggact gaagtcctcc tcagagagct
     4141 tggtctagac ccggaagatg agacccgcca gcaccaatcc cgagacgagc taatagccca
     4201 actggtcgct atgaaactgc cgcttaaccg ttagtagctc acctactcaa tacctaattt
     4261 aaacaccttt tgagatgacc gatgcgggga gacccgtagg ccttactgtg cctgcgatac
     4321 cgatagtctc aactctcaag aggagaatta cctatgatgt tcaacaacaa caactggaag
     4381 ctgtcagtca ccgacatcaa cctttatgaa aacactgtga gtctggacgg tcagtcctat
     4441 ccgttgtcgc tcgctatcaa gaccctcatt ccgggctacc tgtcaggctt accgtccacc
     4501 agccgcgagg cgatggagtt actggaagct ctggctgaag ctggcgtgac cattggtaac
     4561 ttcttcagta atgacctgat gaccgcctac caacgccgcc agatgaacaa acgagctgaa
     4621 gctgagcgta tcgctaaaga gctagcgtct cagaaagagc gtacccgcga aatgttcatg
     4681 accgaagacg aatggcaaaa agagctacag cgccgcgaac aggtcaaagc tgaacgccgg
     4741 acctatggtg aaaacctccg cagcgctact cattccgctg gtcgctcacg tgccgctatc
     4801 gtcgctgacc ttgagtcagg cggtaactgg atggacagct tgtaagcaag cgtccctcat
     4861 ctgagaacac acggtccctg actgcctgtt agggacttca agtatttgac tcaaggagat
     4921 taattataat gaatatgact aaaaacgctt tccagtttgg cattgagcct gttcatatca
     4981 ccgacaacga caaccttcag gttaatgagg gccttccgac taacgccgac cctcaggtct
     5041 atgccctcca gttagctaag actgttaagg ctatgctgaa cggtgtactt aaagacgctc
     5101 aagataacat cccgttccct gtagaagtcc tgccgacccg taacagctta ccgactccaa
     5161 tcattgctca cactctggct gaccgctctg tgctggtccc tgtccgtggt ggtaagcgac
     5221 ctgaagttgt gaccgctccg tctggcactg aaatcaccgt tgagccgatt gaacaggcta
     5281 tcctcgtgtc tcaccagaca aagctttggg accagaagtc gaccactggt ttcactcagg
     5341 ggacgctcca gcaggacgca ctgaacatct gcgataacgt ggttcgtacc attaactcta
     5401 agatggttga cgtgctggag tcctcaaagc tgctgaagac cgtagaactt ccggccctga
     5461 ctggtagcct gaccgctaag gctgacgcta tcatggatgc cctgtacgag aacacggagt
     5521 cttctttcgg ttctgaggtt tctgactacg gcattatcgc ccatgagtca cacctcaagg
     5581 ctctctcccg tctggctgct aagcagggct tcggtggtga agacgctatc gtcgatatgc
     5641 tggggactga cgtcgcctat tacaacggtg aagaccgtgg cgtgttcatg atggctaagc
     5701 gttttacggc tctgagcttc ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc
     5761 tttcacgtga tggagactcc cagtctcacg accttgagat tctcggcaag gtgtttgttg
     5821 tagctgaagc tgctacgacc atcaagatgg gtactggctc ggctactgct gtcctgcctg
     5881 tagtcaaacg cctgagcttc actaaagaag ctaactaaca ataacgacta ttggtcagac
     5941 ttccgaactg gatgtctggc ctttattttg tactagtaat ctagttcgcg aatacagaaa
     6001 gtaggagggc caaatggacc tacacaaagt ctatctcgtg gtcttcctgc taaagctggg
     6061 actggtggtc tggctatggg ctattagacg atgagttttg actgaaaaat gtgagacctc
     6121 atctaatata aagagcgagc caaaagcccc catagcctga cctgagccag accaaaaggg
     6181 ccgggtagtc agaccgtgag ttctattcct ttagagggaa tattacgtgc ttcaagtctt
     6241 gaccttctat ctctaaatgt gtgactatca ctacaaccaa ctggattcac ctgtctttaa
     6301 gaaacaatag gttagctggt tgtcttcgat ttgtcgtagg ctcccgctgc ccgctccagt
     6361 cagagtcttt cagtctgcga tgatgggaaa tcccggagtg actgaaagac gtttaagtta
     6421 tgaatgatcg cctttttttg caaaattgct gtgcagaaat actaaccttc gggcgtgcga
     6481 tcattcataa gcactctgct tttattccga ttaactgtgg gtggtttgtt ggatagagtg
     6541 ctttccttac tgtatatatc gtttcgcccg cttttgcgtt tttttctttt caaatccctt
     6601 tcatttctca gtgtaaaact acgccatccg ttatttgcgg aggtgaggct atgaaatcca
     6661 tggacaaaat ttcaacgggc attgcctacg gcaccgccgc cggcagtgcc ggctactggt
     6721 ttttgcagtg gttggatcag gtcagtccat cacagtgggc tgcgattggt gtgctgggaa
     6781 gtctgcttct ggggcttctg acttatctga cgaatctgta tttcaaaata agagaagata
     6841 agcgtaaggc tgcgagaggt gaataatgtc gccatcatta cgcaaggctg ttgcagttgc
     6901 tattggtggc ggggctgttg ctatagcatc tgtgttaatc actggcccag gtggtaacga
     6961 tggtctggaa ggtgtgagct acataccata caaagatatc attggtgtat ggactgtatg
     7021 tcacggacac accggaaaag acatcatgc
//
LOCUS       DADMFL010000166         6900 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.170, whole genome shotgun sequence.
ACCESSION   DADMFL010000166 DADMFL010000000
VERSION     DADMFL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.170"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..731
                     /locus_tag="JNZ27_003743"
                     /pseudo
     CDS             1..731
                     /locus_tag="JNZ27_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1034..1804)
                     /locus_tag="JNZ27_003744"
     CDS             complement(1034..1804)
                     /locus_tag="JNZ27_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665895.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="JNZ27_003745"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="JNZ27_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="JNZ27_003746"
     CDS             complement(2295..2516)
                     /locus_tag="JNZ27_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665896.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="JNZ27_003747"
     CDS             complement(2517..3200)
                     /locus_tag="JNZ27_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY8665897.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="JNZ27_003748"
     CDS             complement(3264..3575)
                     /locus_tag="JNZ27_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665898.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3572..4543)
                     /locus_tag="JNZ27_003749"
     CDS             complement(3572..4543)
                     /locus_tag="JNZ27_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY8665899.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLIH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="JNZ27_003750"
     CDS             4914..5873
                     /locus_tag="JNZ27_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8665900.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="JNZ27_003751"
     CDS             5873..6268
                     /locus_tag="JNZ27_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8665901.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="JNZ27_003752"
     CDS             6451..6831
                     /locus_tag="JNZ27_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY8665902.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1884 a         1782 c         1676 g         1558 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctgggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcacag gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta gcggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccgcaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcctgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc gtccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acacagatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccagcgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tatcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaagt agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgctt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 tataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gacccccgac tgttaacgct
     6061 aatcagcggt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taatattgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaataacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
//
LOCUS       DADMFL010000167         6886 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.171, whole genome shotgun sequence.
ACCESSION   DADMFL010000167 DADMFL010000000
VERSION     DADMFL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.171"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..789)
                     /locus_tag="JNZ27_003753"
     CDS             complement(<1..789)
                     /locus_tag="JNZ27_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY8665903.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /locus_tag="JNZ27_003754"
                     /pseudo
     CDS             1036..1662
                     /locus_tag="JNZ27_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1659..1865)
                     /locus_tag="JNZ27_003755"
     CDS             complement(1659..1865)
                     /locus_tag="JNZ27_003755"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8665904.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1835..2773)
                     /locus_tag="JNZ27_003756"
     CDS             complement(1835..2773)
                     /locus_tag="JNZ27_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8665905.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNZ27_003757"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNZ27_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY8665906.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="JNZ27_003758"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="JNZ27_003758"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY8665907.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="JNZ27_003759"
     CDS             complement(4852..6180)
                     /locus_tag="JNZ27_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8665908.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..>6886)
                     /locus_tag="JNZ27_003760"
     CDS             complement(6188..>6886)
                     /locus_tag="JNZ27_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8665909.1"
                     /translation="VDINTRAILSAEALCRWVSAERGIISPLKFITIAEDIGFINELG
                     YQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSL
                     CVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQ
                     FTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPM
                     PFSAWDKSGKLVKE"
BASE COUNT         1798 a         1759 c         1586 g         1743 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttgcgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgcttccc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccgtcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaac
//
LOCUS       DADMFL010000168         6850 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.172, whole genome shotgun sequence.
ACCESSION   DADMFL010000168 DADMFL010000000
VERSION     DADMFL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.172"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(68..358)
                     /locus_tag="JNZ27_003761"
     CDS             complement(68..358)
                     /locus_tag="JNZ27_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665910.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            515..1444
                     /gene="metA"
                     /locus_tag="JNZ27_003762"
     CDS             515..1444
                     /gene="metA"
                     /locus_tag="JNZ27_003762"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY8665911.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1707..3308
                     /gene="aceB"
                     /locus_tag="JNZ27_003763"
     CDS             1707..3308
                     /gene="aceB"
                     /locus_tag="JNZ27_003763"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY8665912.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLAELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWCGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3338..4642
                     /gene="aceA"
                     /locus_tag="JNZ27_003764"
     CDS             3338..4642
                     /gene="aceA"
                     /locus_tag="JNZ27_003764"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY8665913.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            4826..6562
                     /gene="aceK"
                     /locus_tag="JNZ27_003765"
     CDS             4826..6562
                     /gene="aceK"
                     /locus_tag="JNZ27_003765"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY8665914.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(6531..6842)
                     /locus_tag="JNZ27_003766"
     CDS             complement(6531..6842)
                     /locus_tag="JNZ27_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665915.1"
                     /translation="MFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQ
                     KYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTV
                     R"
BASE COUNT         1670 a         1757 c         1768 g         1655 t
ORIGIN      
        1 gtgaaaaaaa tgccgggtgc gacgctggcg cgtcttctcc agcttgggca ggcacggcag
       61 gggcggctta ttcccccaca tacgccagat tcagcaacgg atacggtttc cccaaatcgt
      121 ccacctcaga gcgtcccgta accttaaaac ccaccttctt atagaaccca accgcctgct
      181 cccgttcatt aaccgcgacc cacaacggtg cttccggcag aaaggaacgc accagctctt
      241 ccagctcggc ccgatactct gctgataaaa aatcgtgggt ggcatcgaca gatcgacacc
      301 aaatcgcaac gagttcctcc ccttcctcat gccgtgagcg gcgaatacta ataaccattt
      361 tctctccttt tcgtcattct tatattctaa cgtagccttt tccttgaaac tttctcacct
      421 tcaacatgca ggctcgacat tggcaaattt tctggttatc ttcagctatc tggatgtcta
      481 aacgtataag cgtatgtagt gaggtaatca ggttatgccg attcgtgtgc cggacgagct
      541 acccgccgtc aatttcttgc gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc
      601 tggtcaggaa attcgtccac ttaaggttct gatccttaac ctgatgccga agaagattga
      661 aactgaaaat cagtttctgc gcctgctttc aaactcacct ttgcaggtcg atattcagct
      721 gttgcgcatc gattcccgtg aatcgcgcaa cacgcccgca gagcatctga acaacttcta
      781 ctgtaacttt gaagatattc aggaacagaa ctttgacggt ctgattgtaa ccggtgcgcc
      841 gctgggcctg gtggagttta atgatgtcgc ttactggccg cagattaaac aggtgctgga
      901 gtggtcgaaa gatcacgtca cctcgacgct gtttgtctgc tgggcggtac aggccgcgct
      961 caatatcctc tacggcattc ctaagcaaac tcgcaccgac aaactctctg gcgtttacga
     1021 gcatcatatt ctccatcctc atgcgcttct gacgcgtggc tttgatgatt cattcctggc
     1081 accgcattcg cgctatgctg actttccggc agcgttgatt cgtgattaca ccgatctgga
     1141 aattctggca gagacggaag aaggggatgc atatctgttt gccagcaaag ataagcgcat
     1201 tgcctttgtg acgggccatc ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg
     1261 cgatgtggaa gccggactag acccggatgt accgtataac tatttcccgc acaacgatcc
     1321 gcaaaacaaa ccgcgagcga gctggcgtag tcacggtaat ttgctgttta ccaactggct
     1381 caactattac gtctatcaga tcacgccata cgatctacgg cacatgaatc caacgctgga
     1441 ttaatcttct gtgatagtcg atcgttaagc gattcagcac cttacctcag gcaccttcgg
     1501 gtgccttttt tatttccgaa acgcacctca gcaagtgaat aaattttatt catattgtta
     1561 tcaataagtt atcaagtatt tttaattaaa atggaaattg tttttgcatt ttaaatgagt
     1621 agtcttagtt gtgctgaacg aaaagcgcac aacattcctt cgttcacaga ggggaagttt
     1681 tcggatccat gacgaggagc tgcacgatga ctgaacaggc aacaacaacc gatgaactgg
     1741 ctttcacaag gccgtatggc gagcaggaga agcaaattct tactgccgaa gcggtagaat
     1801 ttctggctga gctggtgacg cattttacgc cacaacgcaa taaacttctg gcagcgcgca
     1861 ttcagcagca gcaagatatt gataacggaa cgttgcctga ttttatttcg gaaacggctt
     1921 ccattcgcga tgctgattgg aaaattcgcg ggattcctgc ggacttagaa gaccgccgcg
     1981 tagagataac tggcccggta gagcgcaaga tggtgatcaa cgcactgaac gccaatgtga
     2041 aagtctttat ggccgatttc gaagattcac tggcaccaga ctggaacaaa gtgatcgacg
     2101 ggcaaattaa cctgcgcgat gcggttaacg gtaccatcag ctataccaat gaagcaggca
     2161 aaatttatca gctcaagccc aatccagcgg ttttgatttg tcgggtacgc ggtctgcact
     2221 tgccggaaaa acatgtcacc tggtgtggtg aggcaatccc cggtagcctg tttgattttg
     2281 cgctctattt cttccacaac tatcaggcac tgttggcaaa gggctgcggt ccctatttct
     2341 atctgccgaa aacccagtcc tggcaggaag cggcctggtg gagcgaagtc ttcagctatg
     2401 cagaagatcg ctttaatctg ccgcgcggca ccatcaaggc gacgttgctg attgaaacgc
     2461 tgcccgcggt gttccagatg gacgaaatcc ttcacgcgct gcgcgaccat attgttggtc
     2521 tgaactgcgg tcgctgggat tacatcttca gctatatcaa aacgttgaaa aactatcccg
     2581 atcgtgtcct gccagacaga caggccgtga cgatggataa accattcctg aatgcttact
     2641 cacgcctgct gattaaaacc tgccataaac gcggtgcttt tgcgatgggc ggcatggcag
     2701 cgtttattcc gagcaaagat gaagagcgta ataaccaggt gctcaacaaa gtaaaagcgg
     2761 ataaagcgct ggaagccaat aacggtcacg atggcacatg gatcgctcac ccaggccttg
     2821 cggacacggc aatggcggta ttcaacgata ttctcggctc ccgtaaaaat cagcttgaag
     2881 tattgcgcga acaagacgcg ccgattactg ccgatcagct gctggcacct tgtgatggtg
     2941 aacgcaccga agaaggtatg cgcgccaaca ttcgcgtggc tgtgcagtac atcgaagcgt
     3001 ggatctccgg caacggctgc gtgccgattt atggcctgat ggaagatgcg gcgacggctg
     3061 aaatttcccg tacctcgatc tggcagtgga tccatcatca aaaaacgttg agcaatggca
     3121 aaccggtgac caaagccttg ttccgccaga tgctgggcga agagatgaaa gtcattgcca
     3181 gcgaactggg cgaagaacgt ttctcccagg gccgttttga cgatgccgca cgcttgatgg
     3241 aacagatcac cacttccgat gagttaattg atttcctgac cctgccaggc taccgcctgt
     3301 tagcgtaaac caccacataa ctatggagca tctgcacatg aaaacccgta cacaacaaat
     3361 tgaagaatta cagaaagagt ggactcaacc gcgttgggaa ggcattactc gcccatacag
     3421 tgcggaagat gtggtgaaat tacgcggttc agtcaatcct gaatgcacgc tggcgcaact
     3481 gggcgcagcg aaaatgtggc gtctgctgca cggtgagtcg aaaaaaggct acatcaacag
     3541 cctcggtgca ctgactggcg gtcaggcgct gcaacaggcg aaagcgggta ttgaagcagt
     3601 ctatctgtcg ggatggcagg tagcggcgga cgctaacctg gcggccagca tgtatccgga
     3661 tcagtcgctc tatccggcaa actcggtgcc agctgtggtg gagcggatca acaacacctt
     3721 ccgtcgtgcc gatcagatcc aatggtccgt gggcattgag ccgggcgatc cgcgctatgt
     3781 cgattacttc ctgccgatcg ttgccgatgc ggaagccggt tttggcggtg tcctgaatgc
     3841 ctttgaactg atgaaagcga tgattgaagc cggtgctgcg gcagttcact tcgaagatca
     3901 gctggcgtca gtgaagaaat gcggtcacat gggcggcaaa gttttagtgc caactcagga
     3961 agctattcag aaactggtcg cggcgcgtct ggcagctgac gtgacgggcg ttccaaccct
     4021 gctggttgca cgtaccgatg ctgatgcggc ggatctgatc acctcagatt gcgacccgta
     4081 tgacagcgaa tttattaccg gcgagcgtac cagtgaaggc ttcttccgta ctcatgcggg
     4141 cattgagcaa gcgatcagcc gtggcctggc gtatgcgcca tatgctgacc tggtctggtg
     4201 tgaaacctcc acgccggatc tggaactggc gcgtcgcttt gcacaagcta tccacgcgaa
     4261 atatccgggc aaactgctgg cttataactg ctcgccgtcg ttcaactggc agaaaaacct
     4321 cgacgacaaa actattgcca gcttccagca gcagttgtcg gatatgggct acaagttcca
     4381 gttcatcacc ctggcaggta tccacagcat gtggtttaac atgttcgacc tggcaaacgc
     4441 ttatgcgcag ggcgaaggta tgaagcacta tgttgagaaa gtgcagcagc cggaatttgc
     4501 cgccgcgaaa gatggctata ccttcgtatc tcaccagcag gaagtgggta caggttactt
     4561 cgataaagtg acgactatta ttcagggcgg cacgtcttca gtcaccgcgc tgactggctc
     4621 cactgaagaa tcgcagttct aagcaaccta caaacgttgc tgactgtagg ccggataagg
     4681 catttacgcc gcatccggca atcggtgcac gatgcctgat gcgacgcttg cgcgtcttat
     4741 catgcctaca accgttgccg aacgtaggct ggataaggtg tttacgccgc atccggcaat
     4801 tctctgctcc tgatgagtgc gctaaatgcc gcgtggcttg gaattactga ttgctcaaac
     4861 cattttgcaa ggcttcgacg ctcagtatgg tcgattcctc gaagtgactt ccggggcgca
     4921 gcagcgtttc gaacaggccg actggcatgc tgtccagcag gcgatgaaaa accgtatcca
     4981 tctttacgat catcacgttg gtctggtcgt ggagcaactg cgctgcatta ctaacggcca
     5041 aagtacggac gcggcatttt tactgcgcgt taaagagcat tacacgcggc tgttgccgga
     5101 ttacccgcgc ttcgagattg cggagagctt ttttaactcc gtgtactgtc ggttatttga
     5161 ccaccgctcg cttactcccg agcggctttt tatctttagc tctcagccag agcgccgctt
     5221 tcgtaccatt ccccgcccgc tggcgaaaga ctttcacccc gatcacggct gggaatctct
     5281 actgatgcgc gttatcagcg acctgccgct gcgcctgcgc tggcagaata aaagccgtga
     5341 cattcattac atcattcgcc atctgacgga aacgctgggg acagacaacc tcgcggaaag
     5401 tcatttacag gtggcgaacg aactgtttta ccgcaataaa gccgcctggc tggtgggcaa
     5461 actgatcacg ccttccggca cgttgccctt cttactgccg atccaccaga cggacgacgg
     5521 cgagttattt attgatacct gcctgacgac gaccgccgaa gcgagcattg tttttggctt
     5581 tgcgcgttct tattttatgg tttatgcacc gctgcccgca gcgctggtcg agtggctacg
     5641 ggaaattctg ccaggtaaaa ccactgctga attgtatatg gctatcggct gccagaagca
     5701 cgccaaaacc gaaagctacc gcgaatatct cgtttatcta cagggctgta atgagcagtt
     5761 cattgaagcg ccgggtattc gtggaatggt gatgttggtg tttacgctgc cgggctttga
     5821 tcgggtattc aaagtcatca aagacaagtt cgcgccgcag aaagagatgt ctgccgctca
     5881 cgttcgtgcc tgctatcaac tggtgaaaga gcacgaccgt gtgggccgaa tggcggacac
     5941 ccaggagttt gaaaactttg tgctggagaa gcggcatatt tccccggtat taatggaatt
     6001 actgctccag gaagcagcgg aaaaaatcac cgatcttggt gagcagattg taattcgcca
     6061 tctctatatc gagcggcgga tggtgccgct caatatctgg ctggaacaag tggaaggtca
     6121 gcagttgcgc gacgccattg aagaatacgg taacgctatt cgccagcttg ccgctgctaa
     6181 cattttccct ggcgacatgc tgtttaaaaa cttcggtgtc acccgtcacg ggcgtgtggt
     6241 tttttatgat tacgatgaaa tttgctacat gacggaagtg aatttccgcg acatcccgcc
     6301 gccgcgctat ccggaagacg aacttgccag cgaaccgtgg tacagcgtct cgccgggcga
     6361 tgttttcccg gaagagtttc gccactggct atgcgccgac ccgcgtattg gtccgctgtt
     6421 tgaagagatg cacgccgacc tgttccgcgc tgattactgg cgcgcactac aaaaccgcat
     6481 acgtgaaggg catgtggaag atgtttatgc gtatcggcgc aggcaaagat ttagcgtacg
     6541 gtatggggag atgctttttt gagtaaagct tccatataat ttttctccgc aatgtatcga
     6601 gggttatccg taaagccaaa gctttcagcc atcttattta atgtattaag gattaattca
     6661 gcaataaccc ggtgaccaaa ttcaaaagcc aactcaaagg cagagtattt ttgtggcgct
     6721 ttgtgttgcc aaaaatccat aatatcttca gcggtaaatc caaacaggcg tgcatggtca
     6781 gataaagcaa gataaaccgt ctctacaacg ttttgttgtt tatgctgtat cgctgaaaac
     6841 aaaccgggat
//
LOCUS       DADMFL010000169         6792 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.173, whole genome shotgun sequence.
ACCESSION   DADMFL010000169 DADMFL010000000
VERSION     DADMFL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.173"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            239..2671
                     /locus_tag="JNZ27_003767"
     CDS             239..2671
                     /locus_tag="JNZ27_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY8665916.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4207
                     /locus_tag="JNZ27_003768"
     CDS             2702..4207
                     /locus_tag="JNZ27_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY8665917.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDTYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="JNZ27_003769"
     CDS             4207..5868
                     /locus_tag="JNZ27_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY8665918.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JNZ27_003770"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JNZ27_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY8665919.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..>6792)
                     /locus_tag="JNZ27_003771"
     CDS             complement(6477..>6792)
                     /locus_tag="JNZ27_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665920.1"
                     /translation="FDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
BASE COUNT         2004 a         1612 c         1551 g         1625 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatacctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga at
//
LOCUS       DADMFL010000170         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.174, whole genome shotgun sequence.
ACCESSION   DADMFL010000170 DADMFL010000000
VERSION     DADMFL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.174"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..3524
                     /locus_tag="JNZ27_003772"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JNZ27_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JNZ27_003773"
     CDS             3534..6749
                     /locus_tag="JNZ27_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY8665921.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSPEWQSAITQAINLIGEHKPSVPARTMAALAFIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPSYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSQMPEILVSPPWRGRKKKTSIRHFSLPD
                     LTLAPQTYRSPNKHWTPEQQTAIDHFSAMSFDERLATRGADIFLRELGFDVHSLEFNK
                     RLLNGQLDAFDAQQLQEYTSIGLLRTYHAHQAKSKQYREDATNALLAQDSIALMGGWR
                     TFQHGFYYGNEQRWGIWNLYLIAQMPREMAVSCWQQIVEADFHYTGVEYLLSVLGTDA
                     LPGLNTAFARHPKEIFPLLIHFGATELALPIARVWHLFAGQRNLARQWILQWPEHTAT
                     ALIPLVFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPI
                     EIYPARIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQL
                     KTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTHILAWPQE
                     GKSARAVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEE
                     LADRLVPTLGLNDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQ
                     LKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQ
                     RLIWGVIRQMNRVVYSTPFVWPQRGSSAMRKMSQLTCLRTL"
BASE COUNT         1797 a         1781 c         1634 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADMFL010000171         6780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.175, whole genome shotgun sequence.
ACCESSION   DADMFL010000171 DADMFL010000000
VERSION     DADMFL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.175"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            337..579
                     /locus_tag="JNZ27_003774"
     CDS             337..579
                     /locus_tag="JNZ27_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665922.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGSDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="JNZ27_003775"
     CDS             693..1331
                     /locus_tag="JNZ27_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY8665923.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1328..2065
                     /locus_tag="JNZ27_003776"
     CDS             1328..2065
                     /locus_tag="JNZ27_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY8665924.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2065..4161
                     /locus_tag="JNZ27_003777"
     CDS             2065..4161
                     /locus_tag="JNZ27_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY8665925.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4208..4486)
                     /locus_tag="JNZ27_003778"
     CDS             complement(4208..4486)
                     /locus_tag="JNZ27_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665926.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSILPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4630
                     /locus_tag="JNZ27_003779"
     CDS             4487..4630
                     /locus_tag="JNZ27_003779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665927.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4756..5166
                     /gene="psiE"
                     /locus_tag="JNZ27_003780"
     CDS             4756..5166
                     /gene="psiE"
                     /locus_tag="JNZ27_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY8665928.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNZ27_003781"
                     /pseudo
     CDS             complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="JNZ27_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1697 a         1697 c         1785 g         1601 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcagtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     1861 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     1921 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     1981 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     2041 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     2101 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     2161 gatttcggtg gcgtaggatt attacaaact cccaccgcgc gtatggcacg ggaaggggag
     2221 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     2281 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     2341 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     2401 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     2461 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     2521 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     2581 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     2641 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     2701 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     2761 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     2821 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     2881 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     2941 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     3001 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     3061 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     3121 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     3181 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     3241 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     3301 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     3361 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     3421 gatttgtggc tgacggacca tctactgacc actggcagcc tgtttgcaaa tcttgccaac
     3481 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     3541 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     3601 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     3661 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     3721 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     3781 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     3841 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     3901 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     3961 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     4021 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     4081 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     4141 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     4201 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     4261 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     4321 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     4381 cagcagtacc gagcggacca gcataagcag gaagaataga gactgataac atcaaagcag
     4441 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     4501 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     4561 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     4621 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     4681 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     4741 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     4801 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     4861 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     4921 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     4981 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     5041 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     5101 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     5161 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     5221 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     5281 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     5341 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     5401 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     5461 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     5521 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     5581 ccgagcctga gtgtaaaacg cggtactgag gctaaacata ccgattgcca ttccgagtgc
     5641 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     5701 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     5761 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     5821 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     5881 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     5941 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     6001 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     6061 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     6121 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     6181 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
     6241 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
     6301 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
     6361 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
     6421 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
     6481 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
     6541 taagttttgt ggagcaacaa ggacggtatt gagtgactca acagtaccgg aaataacggc
     6601 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
     6661 actggaatta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
     6721 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
//
LOCUS       DADMFL010000172         6777 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.176, whole genome shotgun sequence.
ACCESSION   DADMFL010000172 DADMFL010000000
VERSION     DADMFL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.176"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..90)
                     /locus_tag="JNZ27_003782"
     CDS             complement(<1..90)
                     /locus_tag="JNZ27_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY8665929.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            146..298
                     /locus_tag="JNZ27_003783"
                     /pseudo
     CDS             146..298
                     /locus_tag="JNZ27_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1679..2626
                     /gene="icsP"
                     /locus_tag="JNZ27_003784"
     CDS             1679..2626
                     /gene="icsP"
                     /locus_tag="JNZ27_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY8665930.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3188..3560)
                     /locus_tag="JNZ27_003785"
                     /pseudo
     CDS             complement(3188..3560)
                     /locus_tag="JNZ27_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3563..3760
                     /locus_tag="JNZ27_003786"
                     /pseudo
     CDS             3563..3760
                     /locus_tag="JNZ27_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3804..3923
                     /locus_tag="JNZ27_003787"
                     /pseudo
     CDS             3804..3923
                     /locus_tag="JNZ27_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4173..5039
                     /gene="ospB"
                     /locus_tag="JNZ27_003788"
     CDS             4173..5039
                     /gene="ospB"
                     /locus_tag="JNZ27_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY8665931.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5368..6108
                     /locus_tag="JNZ27_003789"
     CDS             5368..6108
                     /locus_tag="JNZ27_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8665932.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6461..>6777)
                     /locus_tag="JNZ27_003790"
     CDS             complement(6461..>6777)
                     /locus_tag="JNZ27_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY8665933.1"
                     /translation="KKADIALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         2130 a         1267 c         1316 g         2064 t
ORIGIN      
        1 aactctagga taaatatttc tcactatata ttcagtattt gcagtattta ttggaaatac
       61 gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat ataaacatta
      121 ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact ttatctatcg
      181 ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc gctggcgacg
      241 gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
      301 ctgtgtccat attacgtggg taggctcaca gagcctcact tacgactgcg tccatattat
      361 gtgggtagga tcaataaaga cgttttatat gtgtctgctt gcagccagaa aatttatacc
      421 ctgcaggtat cataaacatg cttgataact taattggggc tccctcttcc ttttatggtg
      481 taattgtttg attgaatact tccggggatt tcagtatgaa atgaagtata tttaatatac
      541 tttacaaaaa gtttgtatta gtggaataag gacaatagct tatccaaact gccaagcctc
      601 tttattataa gtaagatctg gcactggaat attctcaaaa ggactaacat catcaccacg
      661 accttcaacc aaccatttta ttgcatttaa atcccagaat agttcagtag tggtcaattt
      721 gggaaacgca caatattgta cggttacgtt atcgcaatag agtgaactcc cgccctatac
      781 ggcgttttca aggattagtc cttaatcgga caaccacatt aaagcataat aaataattaa
      841 gctttaataa tatactatag atacagttct attataaaaa atactatttt aggtgatgca
      901 ttaaattagt agaatattta ttcttttata aaagcaccaa tgaattgtcc aagtaaacat
      961 gaggtaaaat gtgtagagga taactgagtt tggtcacttt aaccgtatta gtcgcagtct
     1021 gactctcgac tttaaaagga tgggaattac ctgtactgat agtgagaaag ctttccattg
     1081 aaataagtca aataaaagtt gatggcttca gtgctaaagt gaggtgcccg ctagccgtat
     1141 tcactcgaat cggaattcaa tgtattgctt ctgccatttc cataatattt cacgggaaaa
     1201 acaaaatctg aaaaactata cttaagtgtt gtattgagtc agttgagata caaatacata
     1261 tttatcagca cacgttacgt caaatacagg aggttgtcat gttggtactg aaaggcacgt
     1321 tggctattgc agccgtgatg acaatttatt gtcttatcgt tgttcttatg gaacgctttt
     1381 ctgattgatt tcatattggc gagagaacaa gaaggtgagt agaattgtcg cggaatcctg
     1441 aaactatcag cctcaggtat gaacaccaaa aggtaggtag agaaatctat ggcccccgtt
     1501 actttattcg cacaatgagg tattttttta tttttacaaa agttcaataa atattataat
     1561 aaaagatatg ttcttggtaa cataaacaaa tatttaatta aaataatgct tggcattgac
     1621 gtttatctta taaagtaaga agatcatgga catttcaacc aaaaaagtag agttctcgat
     1681 gaaatcaaaa ttccttgtac ttgcactttg tgtacctgcg atctttacta cacatgctac
     1741 cactaactat ccacttttca taccggacaa catcagtacg gatattagtc taggatctct
     1801 gagtggcaaa acaaaagaac gcgtttatca tcccaaggaa ggggggagaa aaattagtca
     1861 actggactgg aaatacagta atgcgactat tgttagaggt ggcatcgatt ggaagctaat
     1921 tccaaaagtg tctttcggag tttccggttg gactacttta ggtaaccaga aagcaagcat
     1981 ggttgataaa gactggaaca attccaatac ccctcaggta tggaccgacc agagctggca
     2041 tcccaatacg catctccgtg atgctaacga attcgagctg aatcttaaag gttggttatt
     2101 aaataatttg gattatcgac ttggactaat agcaggttac caagagagtc gttacagttt
     2161 taatgcaatg ggagggagtt atatttatag tgaaaacggg gggaacagga ataaaaaagg
     2221 ggcacatcct agtggtgaaa gaacaatagg ttacaaacag ctcttcaaaa taccttatat
     2281 tggattaact gctaattacc gccatgagaa ttttgagttt ggagcagaac tgaaatatag
     2341 tggttgggtt ctttcatctg atacagataa acactatcag actgagacaa tttttaaaga
     2401 tgaaataaaa aaccaaaatt actgctctgt tgctgcgaat attggatact atgtcacccc
     2461 cagtgcaaaa ttttatatag aaggctccag aaattacatt tctaataaaa aaggtgatac
     2521 atctctttat gagcaaagta ccaatatatc tggcaccatt aaaaatagtg caagtattga
     2581 atatattggt tttctcactt ccgcaggtat aaagtatatt ttttgaattg gttcaccgag
     2641 ttgattacgt tttcatggct aaaaattgcc gcccattgtg agtcatattc gccctgactt
     2701 tccagaacca tacgaactgc ccgttgacgg acttcggggg aaaaacgtgt atttttagtc
     2761 attctgttta cctctttctc agggcgttta gtctccagga tttccggggc ggttcaaaaa
     2821 actcttcgtt gattactgtg gacttactgt tggcattacc gaccctgaga ccggagaaat
     2881 aagaaccgct tagatcatcg tggctgttct cggggtatca agttacacat gggcagaggc
     2941 tacccaaggt ctactattat cgcctgaaag cgctgatgga acagtgctat cagggacatg
     3001 ctgatgaaaa tacctaaata atcagccagc actctatctt tccaaatcca cagcatagca
     3061 aagagagcaa aagagcctgt aaattcagaa attaaggaaa cgcctcttgg tgcacataat
     3121 ctatcttatg ttaaatgaaa tctaatttct tgattatatg ctggatcgtt cgtgggtggc
     3181 tttgccccta tatttccaga cacctgttat cattggcccg ctgccgcaga tattcccgtg
     3241 gcgagcgata atccagcgca ctatgcggat gccattcgtt ataatgctcg aatgcctctg
     3301 caaggttctt tgctgccgtt aacccgtcag gtttgggcat gatactgatg tagtcacact
     3361 ttattgtttt cacgaagctc tttgctattc tgttactctc cggactccgc accgccgtgt
     3421 tcttcggttc aacaaagtcc caacatccgg gtgaactggc gtgtttcatt agcccggtag
     3481 catgaaccat tatccgtcag tcactccact ggagacgacg gaagatcgtt gccgaagcgg
     3541 tgttccaccg ctcccagcat gtcgccaatg ggagagcatc tacggtctga ttgaactgtt
     3601 tattcaaatt gtccaccgtc tgtcagtacg ctctgaacgt cggctggtta aaacactgct
     3661 cgctgatttt cagagggttc acggtaaaac agcactttta ttccggattg cggaagcggc
     3721 tctgaataat ccggatgggc tggttaaaga ggttgtgtac catctgcata ttgttgaacc
     3781 agatcgcagc ggtaagagaa gttcatcata cttggcgcag ttacgcgatg tttctgctag
     3841 aggtgatgcc gtaaagaacg gcagaacatt accggaacaa gatagcggtc tacctgcact
     3901 ggtatcagac ccggggcttc cccggatgat ctccacggta ctttaggtac tgtgtctata
     3961 ttatgtaggt aggatcaatt aactttgtag ttgaaattct ttcattgaaa taatgaataa
     4021 aaaagacaaa ccgcattaaa caatgccaga atataattgc atttttgttc cgttttttta
     4081 aatcatagta tacaaagaag cttgcatact atatgaaaat ataacaccct ttactatgat
     4141 gtttataaaa caatatatgg agtcatgtag gtatgaattt agatggtgtt agaccatact
     4201 gtagaatagt caataaaaag aatgaaagca tatcagatat tgcatttgca catataataa
     4261 aaagggtaaa aaattcatca tgtactcacc caaaagcagc attggttttt ttaggagaga
     4321 aaggtttttg tgatagcaat gatgttctat ctattatggg acaacaaata ccaagagtat
     4381 ttaagaacaa gatgttatat gattatgttt ttaaaaatga aaaaagtaaa aatgattttc
     4441 taaaaatggc tgaatcatgg ctaccacaga gtgaaccaat agtaataaat aatgatgatg
     4501 acgcattgaa tgctgctgct tatttttctg taaaaaaagc gaaaataaaa acagtaaacg
     4561 atactgattt taaagagtat aataaggttt atattcttgg gcacggtagt cctggttctc
     4621 atcaattagg ccttggttcg gaacttattg atgtacaaac aatcatttca agaatgaaag
     4681 actgtggtat tctaaatgtg aaagatatcc gttttacttc atgcggctcc gctgataaag
     4741 tggctcctaa aaattttaac aatgcccctg ctgaaagtct ttcttgtatc cttaactctc
     4801 tgcctttttt taaggaaaaa gaatctttgc tagagcagat aaaaaaacac cttgaaaacg
     4861 atgagtcatt gagtgatggt ctaaaaatat ccggctatca tggatatgga gttcactatg
     4921 gtcaagagct ttttccctac tcacattatc gttcaacttc aattcctgct gatccggagc
     4981 atacagtaaa aagaagctct cagaaaaaga cttttattat taataaagaa ctggattagt
     5041 ataaaatttt taacctatag ttaattgttt gcatttaaga tgaagttatt ttatctttag
     5101 tagaaaagtg cttccctatg agggaaaatc acaaatcatc ataatacaga gacaaaacga
     5161 tacgaacaaa tagataaaaa acatcattct tttaccacat tatttttcgt gaatatgaaa
     5221 aataatgtta ttactttaat ataagactat tttttgtttt tccatcactc tgttcaaatt
     5281 tttccgcatg acttgtgttt tttgtaacac agctcgtttt ttacagctga ccaaaatcat
     5341 caattaatta tgctaaggaa ataaattatg aaaaccaaaa actttcttct tttttgtatt
     5401 gctacaaata tgatttttat cccctcagca aatgctctga aggcagaagg ttttctcact
     5461 caacaaactt caccagacag tttgtcaata cttccgccgc ctccggcaga ggattcagta
     5521 gtatttcagg ctgacaaagc tcattatgaa ttcggccgct cgctccggga tgctaatcgt
     5581 gtacgtctcg ctagcgaaga tgcatactac gagaattttg gtcttgcatt ttcagatgct
     5641 tatggcatgg atatttcaag ggaaaatacc ccaatcttat atcagttgtt aacacaagta
     5701 ctacaggata gccatgatta cgccgtgcgt aacgccaaag aatattataa aagagttcgt
     5761 ccattcgtta tttataaaga cgcaacctgt acacctgata aagatgagaa aatggctatc
     5821 actggctctt atccctctgg tcatgcatcc tttggttggg cagtagcact gatacttgcg
     5881 gagattaatc ctcaacgtaa agcggaaata cttcgacgtg gatatgagtt tggagaaagt
     5941 cgggtcatct gcggtgcgca ttggcaaagc gatgtagagg ctgggcgttt aatgggagca
     6001 tcggttgttg cagtacttca taatacacct gaatttacca aaagccttag cgaagccaaa
     6061 aaagagtttg aagaattaaa tactcctacc aatgaactga ccccataaag ctggacagcc
     6121 tgtatcaggc tatggagggc ccatagacaa atctacccta tatgagcata ggaggagtct
     6181 atgggcacac cacgttttac ccctgaattt aagggattac tggaaaggct gggatatatc
     6241 cctccggcag aagcagaaaa agcttattat gctgccatcg gaaacgatga tctggcaacc
     6301 tgagttcaca gataaaacat tctctaggaa actcggggcg gttccgttca ccacatgcaa
     6361 tgtggtgttg caggggaacg gtctgcacca tcccctatat cgatcaatat aacaaaaatg
     6421 acaacttcag attcagggca caacctaaat atattttagg tcacctctca aatcgtttgc
     6481 ctgataccgc tccatttttt aataaaaaat caatcatttt tgaatctttg cttttcattg
     6541 cattgtcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt acatcaacca
     6601 atccattatt gataaaatat tctagcactt tatagttggc accatcttta ctaagtaagt
     6661 attcaacatc atacagatta caatatatat tgttgttcat tttctccatc tctgcgatat
     6721 cttgttttgt aaaattaaat ttagatagta aataaagtgc tatatcagct tttttat
//
LOCUS       DADMFL010000173         6673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.177, whole genome shotgun sequence.
ACCESSION   DADMFL010000173 DADMFL010000000
VERSION     DADMFL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.177"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..171)
                     /locus_tag="JNZ27_003791"
     CDS             complement(<1..171)
                     /locus_tag="JNZ27_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8665934.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="JNZ27_003792"
     CDS             complement(321..482)
                     /locus_tag="JNZ27_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665935.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(479..682)
                     /locus_tag="JNZ27_003793"
     CDS             complement(479..682)
                     /locus_tag="JNZ27_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY8665936.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            927..1262
                     /gene="iraM"
                     /locus_tag="JNZ27_003794"
     CDS             927..1262
                     /gene="iraM"
                     /locus_tag="JNZ27_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8665937.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1674)
                     /locus_tag="JNZ27_003795"
     CDS             complement(1543..1674)
                     /locus_tag="JNZ27_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY8665938.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKMPDC"
     gene            complement(2174..2250)
                     /locus_tag="JNZ27_003796"
     tRNA            complement(2174..2250)
                     /locus_tag="JNZ27_003796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="JNZ27_003797"
     tRNA            complement(2352..2427)
                     /locus_tag="JNZ27_003797"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="JNZ27_003798"
     CDS             complement(2571..3392)
                     /locus_tag="JNZ27_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8665939.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="JNZ27_003799"
     CDS             complement(3407..3763)
                     /locus_tag="JNZ27_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8665940.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..4825)
                     /locus_tag="JNZ27_003800"
                     /pseudo
     CDS             complement(3776..4825)
                     /locus_tag="JNZ27_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            complement(4827..5105)
                     /locus_tag="JNZ27_003801"
     CDS             complement(4827..5105)
                     /locus_tag="JNZ27_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665941.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(5172..5422)
                     /locus_tag="JNZ27_003802"
                     /pseudo
     CDS             complement(5172..5422)
                     /locus_tag="JNZ27_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="JNZ27_003803"
     CDS             complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="JNZ27_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY8665942.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5867..6154)
                     /gene="relE"
                     /locus_tag="JNZ27_003804"
     CDS             complement(5867..6154)
                     /gene="relE"
                     /locus_tag="JNZ27_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY8665943.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6154..6393)
                     /gene="relB"
                     /locus_tag="JNZ27_003805"
     CDS             complement(6154..6393)
                     /gene="relB"
                     /locus_tag="JNZ27_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY8665944.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6418..>6673
                     /locus_tag="JNZ27_003806"
     CDS             6418..>6673
                     /locus_tag="JNZ27_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665945.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
BASE COUNT         1773 a         1782 c         1399 g         1719 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccattttact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc cccttcggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcatttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acttggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtgc actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgttcaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agatttattg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 actaggataa ttcagctaga gataattcac gctcctgcgt accgcttatt gcgtgaccga
     4381 tgacgtcaat catccatgct gacaggtttt gatgagcaag ttgctcgagt gattcggatg
     4441 tctggtcacg cagctggttg tcgcagtgcc agcacaacac cattgcgccg gtaccataac
     4501 ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag gatttaatat
     4561 ggcgcaacag ccagtcagac aatgcaccag caccaccagc agcacgaatc acctgtgcgt
     4621 tactgaaaaa cggcagcaat gttttgtctt ccaccagcgg ctggcgagcg gcaggaacga
     4681 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtaac cgggtattgt
     4741 ggaataccgg catggattca cgaccgggct taaggaccac cagcccgagt tccggaacca
     4801 gaacaggtcg aagtaatacc cgcacgttac ctccagatcc gttgctggaa tgtgcgggac
     4861 ggacgcggtg gctgttcgga gtaagggagc ctgacggaga ttatccagtg acggtagtcg
     4921 aggctaaggg cttttttaac ctcgcatccg cgcctgcggt aacactgaat gagccattcg
     4981 gcctgttctt cagtgcatga gggatgctgg taccagtctg acttaaatgc gtgagaatac
     5041 cgctcgtgcg tgtgggcaag aacggtcgaa ttatcatgat tgtaatattt tgcgttgcgt
     5101 gccatcggtt ttctccggtg gcacggtgtt actcagcggg agttcagccc cgcgcaagat
     5161 tgtagatgag tttattcttc tgaaaaagca gaaaagccag cttttattcc gatctctttc
     5221 aatgcctgta atgaagtgac aaactcaccg tcgcgcaaga taaatccgtc cgtcactcgg
     5281 gcatccacaa aattaattaa cgcagcccca ttttttgcaa acacacaatg cggtaatgac
     5341 taacaatatt tccattttca acgcacacag catagaggcc atcttcacaa aaaattttac
     5401 gcagttcttc gatgttcatc atcagaatcc ttccagataa gtagcactcc cctgttcggg
     5461 gtccatccct tttatccctg cgcgctactt aagtattttt gattctattc cggcaccatc
     5521 cagaacttca aatgcgttga aaataaaaac aaaaacccgc caaagcgggt taagtgcggg
     5581 tgcgttgagg atgcctgcca catcagaggt ggcgagggat ttctcactcg ccgggtctct
     5641 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt
     5701 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
     5761 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctg tcaacgcaaa
     5821 gcagaagtgt caccttcggt gcgaaacaga gatgtcatgc tttggttcag agaatgcgtt
     5881 tgaccgcctc gctatatact tccgagcgtt ctcttttccc aacagaaatc acgaaaacga
     5941 caactttctc gtctataacc tggtatacaa ggcgatagcc tgaagaccgg agcttaatct
     6001 tgtaacaatc aggcatacca cggagcttgt ttgcttcaat ccggggtgac tcaagtactt
     6061 caaccagctt ctttttcaac tgttcacgta ccgtcgagcc cagctttcgc cattccttta
     6121 gtgcccgctc gtcaaaatcc agaaaatacg ccatcagagt tcatccagcg tcacacgtac
     6181 tggcttagga ttacgaagcc gttctttcac tatctccaca agttcagcat cttcatcact
     6241 caggagtgtc tgtttgaacg gcaagcgttc attgtcagcg atatactcga gcatgagacg
     6301 aagcgcttca gaaggagtta cacccatttt ttcaagcgcg gcgtaagaac gcgctttaag
     6361 ttcatcgtca atacgcaggt taatgctacc catgtcttac acctcttgta attacaaatg
     6421 tcattacaag tatcgcacta caacatgctt agggcaagtc acgaaggaag tcagaaagta
     6481 gtcgtaagaa cggtgatcac tgtccgcttt gtgccaggag cagccattgc taagtccatc
     6541 ctgtattgtg caggtcagct cttttttaaa gagtccggcc atcatcttac tggtacagac
     6601 accatatact ttgtgacggt caggctacat atgcacaact caacttattc atctattttt
     6661 tgctttagca tgt
//
LOCUS       DADMFL010000174         6268 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.178, whole genome shotgun sequence.
ACCESSION   DADMFL010000174 DADMFL010000000
VERSION     DADMFL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.178"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..366)
                     /locus_tag="JNZ27_003807"
     CDS             complement(<1..366)
                     /locus_tag="JNZ27_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665946.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            891..1865
                     /gene="hemB"
                     /locus_tag="JNZ27_003808"
     CDS             891..1865
                     /gene="hemB"
                     /locus_tag="JNZ27_003808"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY8665947.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHSVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1971..2822)
                     /gene="tauD"
                     /locus_tag="JNZ27_003809"
                     /pseudo
     CDS             complement(1971..2822)
                     /gene="tauD"
                     /locus_tag="JNZ27_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2819..3646)
                     /gene="tauC"
                     /locus_tag="JNZ27_003810"
     CDS             complement(2819..3646)
                     /gene="tauC"
                     /locus_tag="JNZ27_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY8665948.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFIVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3643..4410)
                     /gene="tauB"
                     /locus_tag="JNZ27_003811"
     CDS             complement(3643..4410)
                     /gene="tauB"
                     /locus_tag="JNZ27_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY8665949.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGCVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4423..5349)
                     /gene="tauA"
                     /locus_tag="JNZ27_003812"
     CDS             complement(4423..5349)
                     /gene="tauA"
                     /locus_tag="JNZ27_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8665950.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVRAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5616..6122)
                     /locus_tag="JNZ27_003813"
     CDS             complement(5616..6122)
                     /locus_tag="JNZ27_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665951.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQSDLSQGDQLTEMLSFLTSEEREAL
                     TNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEASILS
                     ESVNAKAG"
BASE COUNT         1537 a         1674 c         1514 g         1543 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaata ataaccaaga gctataaata
      721 aagattaaaa atattttcag tgaatatgaa attattgcta tttctattac aaagatgtcc
      781 tggtctctct ttcattctct tcttgtcaac atcgcgacaa ctttcgtaaa acactcctgc
      841 cccgcttcag gtatactgtg caccttaatt ccacaaacat caggcagacc atgacagact
      901 taatccaacg ccctcgtcgc ctgcgcaaat ctcctgcgct gcgcgctatg tttgaagaga
      961 caacacttag ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa attgacgact
     1021 acaaagccgt tgaagccatg ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg
     1081 aaattgaacg catcgccaac gccggtatcc gttccgtaat gacttttggc atctctcacc
     1141 ataccgatga aaccggcagc gacgcctggc gggaagatgg actggtggcg cgtatgtcgc
     1201 gcatctgcaa gcagactgtg ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat
     1261 acacttctca cggtcactgc ggtgtgctgt gcgagcatag cgtcgacaac gacgcgactc
     1321 tggaaaattt aggcaagcaa gccgtggttg cagctgctgc aggcgcagac ttcatcgccc
     1381 cttccgccgc gatggacggc caggtacagg cgattcgtca ggcgctggac gctgcaggct
     1441 ttaaagatac ggcgattatg tcgtattcga ccaagttcgc ctcttccttt tatggcccgt
     1501 tccgtgaagc tgccggaagc gcattaaaag gcgaccgcaa aagctatcag atgaacccaa
     1561 tgaaccgtcg cgaggcgatt cgtgagtcac tgctggatga agcccagggc gcagactgcc
     1621 tgatggttaa acctgctgga gcgtacctcg acatcgtgcg tgagctgcgt gaacgtactg
     1681 aattgccgat tggcgcgtat caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc
     1741 tggcgggtgc tatagatgaa gagaaagtcg tgctcgaaag cttaggttca attaagcgtg
     1801 cgggtgcgga tctgattttc agctactttg cgctggattt ggctgagaag aagattctgc
     1861 gttaattttt ttcctctcag aaagggggag ttaacctgtt gaaagtacgg tgaacgcccc
     1921 ctcaccctaa ccctctcccc aagggggcga gggaaccgtc cacactcgta tcaccccgcc
     1981 cgataaaacg gtttatcccc aaggatcgtc gcccgatgca taatccgccg ctgtggcagg
     2041 taatcggcat tggcatagtg ctgagtcacg cggttatccc aaatcgcaat atcattcggt
     2101 tgccagcgcc aacgcacctg aaactccggt ttagtgatat gggcgaataa aaagcctaac
     2161 aaggcttcgc tctctttctc gctgacatcg acaattcgcg tagtaaagcc ttcattcaca
     2221 aacagcgcct gtttaccgct caccggatgc gttcgcacca ccggatgtag caacggagga
     2281 tttttcgcga ccgcctcgcg ccagcgttga tgttcctcct cggttttgcg gtatttgtat
     2341 tccgggaatg atttacggaa atcatgctcc gcacgcagcc agctcagcag ctggcggaag
     2401 ggaacagaga gcgcctcata ggccgcaata ccgctggttc agagcgtatc accgccggtc
     2461 gaaggtaact ctttagctgc cagaatcgcc cctgcgggtg gcgtttcaat aaatgtcaca
     2521 tcggtatgcc agttgtcgtt atccggcgga ttatcgttat gggtatccag cacgatgatc
     2581 tcgtcaaccc cttcggcatg agggtaaaca gggtgaatat gcagttcgcc aaaacgctgg
     2641 gccagcgcgc gttgctgctg cggcgtaata gcttgatcgc gcagaaatac cacctgatgg
     2701 cgcagcaccg catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgtcaggtcg
     2761 gcacccgaaa tttgtgcgcc gatatgcggc cccagcgggg taatgctcag acgttcactc
     2821 attgtacttc tccatgccag ggcgtcagac ggcgctgtaa cgcgcgcaga cccagttcta
     2881 aaagaaaggc gataatcgca atcaccgcga tccccgccag taccacgtca gtcgcgagaa
     2941 attcacccgc tgactgaact ataaatccta aaccgcgtgt cgcggcaatc agctccgccg
     3001 ctaccagcgt agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca
     3061 gcgcgccggg caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg
     3121 cggcacgaat gcgaacctgc tgcgcgcttt tcacccctgc cagcgccgac atcgccaccg
     3181 gcgcaaaaat cgctaaatag atcagtaaga tcttcgaggt ttcaccaata ccaaaccaga
     3241 tcaccatcag cggcaaataa gccagcggtg gcaccggacg ataaagttcg attaccggat
     3301 ccagaatgcc gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca
     3361 ccaccgccgc gagtagcgcc tgcacaatgc gcgtcaggct ggctgccaga tgctgccaca
     3421 gcgtggcgtc cataaatccc tgcggtccgg caatggtgag cagttttgcc agtacttgtt
     3481 gcggcggcgg caaaaacagc gggctaatca gttgcagcgc cgccaccgtc caccatacag
     3541 tgagtaaaac cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc
     3601 atttcagccg ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg
     3661 ttgctcaaat acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt
     3721 gatgctgcgg ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc
     3781 cagcacacag ccagggccgg atgaaagcag aaccagttca gtcgccataa acaccgcttc
     3841 ttctatatcg tgggtaatca acagcacctg cttgcccgtc tcctgccaga gtttcagcag
     3901 cagagtttgc atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt catcgagtaa
     3961 taacagctgg ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac gttgaccatc
     4021 ggaaagctgc cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg
     4081 gtgcgcgatt tccagtcgct gcattttctc tatacctgcc aattgcaggc cgaacgccac
     4141 gttgtcctgt acattgcgcc acggtagcag cccttcattc tgaaaaacta cgccacgctc
     4201 tgctcccggt ccctcaatac gcttacccgc cagttgaatg ctgccatgct gataaggcac
     4261 aaaaccggca atcagattca gcagggtggt tttaccgcag ccggacggcc ccagcaccac
     4321 cagtagctcg ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata
     4381 atcggcatag agatgagaga tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga
     4441 ggtaacgtac tggctgtaat cattcgctac agccgggacc ttgccctgct ctttcaaaaa
     4501 ctgcgcggtg tcgatgattg ctttgttcac cggtccggtc agttctgccg tttgttgctg
     4561 cggcgtcaga taggtatttc ccttcaccag ccccggaacg tcaccttcag gcacgccgct
     4621 taaacgcgcc agtttgctga tgttttccgg ctgtttcagc cacgcgtcag ggttagcaat
     4681 gtacggttgc tgtgcatcga tggcgctttt ggcgaacgct ttcacgacct caggatgttt
     4741 ctcggcaaaa tctttgcgca ccacccagac gtccagcgtt ggcgcacccc actgcccgac
     4801 ctgttcagaa tcggtcagca ccttgccgtc tttttccagg gcgttaaccg ccggtgccct
     4861 gacataagca ccatcaatat caccccgctg ccatgcggcg ataatcgcgg gcggctgcag
     4921 gttcacaatc tccactagcc ctggtttaat cccccagtgt ttcagcgccg ccagcaggct
     4981 gtagtgggtg gtggagataa acggtacggc aatgcgttta ccaatcaaat cttcgggttt
     5041 gctgatagtt ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa
     5101 gacttcaatc ggcacctgtt ggctggctgc aaccgctaat gggctggaac cgaggttgcc
     5161 gatttgcacg tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg
     5221 ccagtccacg ctcgccccgc tctctttagc aaaggtgttg tcggcctgag ccactttcgc
     5281 cggttcggct gaggtttgat acgccacggt gacgttcacc gcctgtgcct gaaaagcgat
     5341 gaatgccagt gcggcaagaa gtgtgttata tgactaggta atttattcaa gataaccggg
     5401 aatgggtaaa aaaagacggg atttgatggg attcaataag ttatgcgccg attgcattca
     5461 agataaaacg agttaaaaat gctcaatgct tcgatatcga taaaacattc aagataggcg
     5521 cgccgtgtat tttaattctt ttaagactct tcaaaaatgt catcgtagcc tttaaaaacg
     5581 aattttaaag gcaccctaaa ggcaggtcaa tgaagttaac ccgccttcgc gtttacactc
     5641 tctgagagaa tactagcttc aatctcttcc aaaatctctc tgacttgctc gtcgggcata
     5701 tcaacgagca tcaacgctgc caggcgttta cggccctcta gttgcagtag ctcttgagtt
     5761 tccttatcta acaagcgtag cacagtctca acacctttcc ttgcgagcat gtttgtaagg
     5821 gcttctcgtt cctcactggt aagaaacgac aacatttctg ttagctgatc cccttggctt
     5881 aaatcacttt gtgtttttgg tggattcccg ttaccggttt ttagccattc aagactaaca
     5941 ccttcacttt ctgcaatgcg taccaatatg ctttctcgag gttctggtgt atttcccttt
     6001 ttaaaatagc tttgtaacgt attgatattt atgccacagg ctctggcggc agcacttctt
     6061 gatggatacc gcctaataag ataagccatg cgctccacga taggttcttt tgcctgctcc
     6121 ataaaagtac ctctaggtat ttttctgtat aaaggttctt ttccatctat ctaattgaat
     6181 ggaaaggtaa ttttaaaaat ccaaataaaa tacaaaaaaa taccttgtat tggtgctttt
     6241 tgtgatctgc gattttattt actcggat
//
LOCUS       DADMFL010000175         6200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.179, whole genome shotgun sequence.
ACCESSION   DADMFL010000175 DADMFL010000000
VERSION     DADMFL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.179"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="JNZ27_003814"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="JNZ27_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY8665952.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1560..1964)
                     /locus_tag="JNZ27_003815"
     CDS             complement(1560..1964)
                     /locus_tag="JNZ27_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
                     /protein_id="HAY8665953.1"
                     /translation="MLHVAGEKRQHRRVSRICDTGDIPLPRSLTRYARSFGCGERYRL
                     TDGDGNFLEDTRKTLSKEEIRPRRSRFSIGSAPLTSIAKSDAQISGGETRQDYKDPRR
                     FPLVAPSCALLFLPFGLPVSFRCYGRGFSFHA"
     gene            complement(2983..3471)
                     /locus_tag="JNZ27_003816"
     CDS             complement(2983..3471)
                     /locus_tag="JNZ27_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HAY8665954.1"
                     /translation="MFCCYNTVHTGDTVTFMAWHTVQETLKLTGKSRSQLYRDMAKGL
                     VSYRTGKNDRREFETSELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKL
                     DALQQTVTLMLEDKMAREAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSG
                     SE"
     gene            3641..4030
                     /locus_tag="JNZ27_003817"
     CDS             3641..4030
                     /locus_tag="JNZ27_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665955.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4613
                     /locus_tag="JNZ27_003818"
     CDS             4074..4613
                     /locus_tag="JNZ27_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8665956.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(4585..5421)
                     /locus_tag="JNZ27_003819"
     CDS             complement(4585..5421)
                     /locus_tag="JNZ27_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY8665957.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="JNZ27_003820"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="JNZ27_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY8665958.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1432 a         1617 c         1642 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcactta
     2581 tacatcactt ttacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DADMFL010000176         5995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.180, whole genome shotgun sequence.
ACCESSION   DADMFL010000176 DADMFL010000000
VERSION     DADMFL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.180"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            73..378
                     /locus_tag="JNZ27_003821"
     CDS             73..378
                     /locus_tag="JNZ27_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8665959.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="JNZ27_003822"
     CDS             complement(454..918)
                     /locus_tag="JNZ27_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8665960.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPFEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /locus_tag="JNZ27_003823"
     CDS             complement(915..1790)
                     /locus_tag="JNZ27_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY8665961.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLVGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="JNZ27_003824"
     CDS             complement(1787..2476)
                     /locus_tag="JNZ27_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY8665962.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JNZ27_003825"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JNZ27_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY8665963.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNASIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JNZ27_003826"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JNZ27_003826"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY8665964.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JNZ27_003827"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JNZ27_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY8665965.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1494 a         1647 c         1521 g         1333 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgaaggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aactaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctag ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgtgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattga agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttca gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaa
//
LOCUS       DADMFL010000177         5994 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.181, whole genome shotgun sequence.
ACCESSION   DADMFL010000177 DADMFL010000000
VERSION     DADMFL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.181"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..268)
                     /locus_tag="JNZ27_003828"
     CDS             complement(<1..268)
                     /locus_tag="JNZ27_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8665966.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHTDAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JNZ27_003829"
     CDS             805..2706
                     /locus_tag="JNZ27_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY8665967.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JNZ27_003830"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JNZ27_003830"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8665968.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JNZ27_003831"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JNZ27_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY8665969.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JNZ27_003832"
     CDS             complement(4548..4757)
                     /locus_tag="JNZ27_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665970.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JNZ27_003833"
     CDS             5041..5403
                     /locus_tag="JNZ27_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY8665971.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1564 a         1453 c         1489 g         1488 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggtat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcgggc tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gagatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctc
//
LOCUS       DADMFL010000178         5992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.182, whole genome shotgun sequence.
ACCESSION   DADMFL010000178 DADMFL010000000
VERSION     DADMFL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.182"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            66..2278
                     /gene="tssI"
                     /locus_tag="JNZ27_003834"
                     /pseudo
     CDS             66..2278
                     /gene="tssI"
                     /locus_tag="JNZ27_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            2301..2745
                     /locus_tag="JNZ27_003835"
                     /pseudo
     CDS             2301..2745
                     /locus_tag="JNZ27_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2767..3166
                     /locus_tag="JNZ27_003836"
                     /pseudo
     CDS             2767..3166
                     /locus_tag="JNZ27_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            3163..5992
                     /locus_tag="JNZ27_003837"
                     /pseudo
     CDS             3163..5992
                     /locus_tag="JNZ27_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108985.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1435 a         1497 c         1842 g         1218 t
ORIGIN      
        1 tgattactta tattttttgg ccaccagggg ctagatttta atccggtatt aaaggagcaa
       61 atatcatgag ttttgtatct acgggtaata aaccagttgg aaacggtgga cccgtcatca
      121 ccacaccacc aatagctggc gaaagtggcg gtatgagcac aggttcagct gtaaccgatg
      181 tatctggtgc cgccgaggag atggcagaac aggcagcggc ggacctgttt ggtgcgctgc
      241 cggaaccgtc aggtctggtt aaagccgcag tagctgctgc gcaggctgcc gctgcagcag
      301 cagggatctc ggatatggct ggtgcagttc aggatgcggc ggcgtcgctt gcagccggtg
      361 gtccgggggc gcataacgtc acagtaagtg gtagtgcggt gccgccgcag atgcttttgt
      421 ttgcaggcat gaatggttcg gaagggcttg ggaatctgtt ttcttacaca gtacagctta
      481 aaacgctgga cgccctgaac ctggggtacg tttcccccgc tgctaacctg ccgcttcagc
      541 tcatggtggg caaagacctg tgcgtcagta tcgaactgga cggcggcgga aaacggtata
      601 tcagcgggct ggtgacggcg gcgagggtgg tcggccatga gggccgttcg gtaacgtatg
      661 aattacgcat tgagccatgg ctgaagctgc tgacccatac cagcgactac aaagcgttcc
      721 agaacaaaac cgtggtggat attctcgatg aagtactgga tgaatattcc tttccggtgg
      781 aaaagcggct ggtggaaaat tatccacccc gcgcctggca ggtgcagtat ggtgaaaccg
      841 acttcgattt catccagcgc ctgatgcagg aatgggggat ttactggtgg tttgagcaca
      901 gcgagaacag ccacacgctg gtgctggtgg atgccatcaa tggtcataaa gcgtgccctg
      961 actcgccgct ggtggagtgg caccaggaag ggctgaaact ggataaggaa tttatccaca
     1021 ccattacggc cagtgagagg ctgcgtactg gaaagtgggt gatggacgat tttgatttta
     1081 tgaagcctcg ttcgctgctt aaaagtaccg tggccagtcc gcgtgatacc ggtcatgctg
     1141 aatacgagca ttacgaatgg cctggtgatt acttcactac gggcgaaggc gagatgctga
     1201 cgcgcattcg tatggaagcg cagcgcagcc caggcagccg gcacatgggg ccggtcatat
     1261 ccgtaccctc atgaccgggt acacctttac gttgatgaat cacccgacgg cagaaattaa
     1321 tcaggaatat ctgctggtac agaccactct gtttttacgg gataatgcgc agcacagcgg
     1381 tcaggatcag cattttacct atgtcaccac gtttgaactg ccccccacca gcgaagttta
     1441 ccgcccacaa cgaaccctga gcaaacccca caccaaaggc ccgcagagcg ccatcgtcac
     1501 cggcccggcg gggcaggaaa tctggacgga taaatatggc cgggtgaagg tgcagtttgg
     1561 ctgggaccgt tacggaaaga atgacgaaaa cagttcctgc tggatccgcg tcagctatcc
     1621 gtgggcgggt aaaggattcg gcatgatcca gatcccgcgt attggccagg aagtactggt
     1681 ggatttcaaa aacggcgatc cggatttgcc catcatcgtg gggcgcacct ataaccagga
     1741 caccatgccg ccgtgggggc tgccgggcat ggcgtcgcag agtgggatat tcagccactc
     1801 attgcaggga gggccgacta acggaaatat gctgcgcttc gatgataaaa cgggtgcgga
     1861 agaggtgaag ttccatgcgg aaaaagacct caataccacg gtaaaaaaca atgaaaccca
     1921 cacggtcatg gtcgatcgca taaaaaccat tgtcaaaaat gaaaccatca gcgtgggaga
     1981 agaccgtaat gccaccatca caaaaaatga tggtctttcc gtcaaactgg ctcaaaccat
     2041 caatgtcgga acgacatatc gtctggatgt tggcgatcaa tttacccttc gttgcggcaa
     2101 tgcagcgctt gtattacaca aggatggctc gattgagttt tgcggaaaac aactgttact
     2161 gcacaccagc gatgttatgc aactcattgg cagtggcatt gatatgaatc cggatggcgg
     2221 cacagccatg actgcggatg atattgcccc gttacctacc cccgacgttt ctgaataaaa
     2281 aatataaaag gaatttttct atgaccagcc tttaccgtat tcaggagggc tgctttgccc
     2341 tgcctgaaac ttttcttgat cgcaccgtca atatcttcgt cccatcgggc aatgaaaggg
     2401 caactcccag tctgaatatt tcccgcgata cgctcaggcc agatgaaagt ctgaccacct
     2461 atattgaccg ccagatagcc ttgatgaaaa aaaaacctgg gccagcaccg ggtactttcg
     2521 cggggaacgg cactggcggg aactggcgaa gctgccatga caggtgaaca gattctcgca
     2581 actcatcgtt ctggaaaaac agaagtgtac cagcgtcagg ccgggtttat caccgggcca
     2641 gcaaaggtac taatgttgac gctgaccact cagcgtcctt ttgatgatca caccgaccag
     2701 ctatggactg catggttgac tagttttcag cctgctaaaa gctaaaaaaa acagggaggt
     2761 tttgtcatgt atgaagccgc acgggtggat gaccccatct accacaccag cgcccttgcc
     2821 gggtttttga taggggccat cataggtatc gccattattg ccgtcgccag ctttgctttt
     2881 tttacctgcg gctttttggc cgggctgatt ttgagttttc tggctgatca gatagcttcc
     2941 ggagtcctgc aactgggtga ggctattggg cgctcgatac attccacagt gggaaaaatt
     3001 ctgaccggtt ctgataatgt cagcaccaac agtcgcccgg gccgtactga gtacggtgat
     3061 atgcgaagac cattccccgg aaatccgcat tgcccagggt tccggcaaca tctacatcaa
     3121 cagccagctg cccgtaagga tgaccacact gagtgtgatg cagtgattga ggacggctcc
     3181 cccaatgtct ttctcggcgg cggtacgcag acggtactgg aaatcagccc ggaaatccct
     3241 gactggctgc gtcaggtggt ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg
     3301 gctggcgcct ggcgtcaggc ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca
     3361 aaatttattg gtggcgaaat ggtcgggatg gccatctctg acacagtcat gggattgttc
     3421 agcaatccgg tagatgtcac cactgggcaa aaaattttgc tgccggagac ggattttacc
     3481 ctgccaggac gcctgcctgt cacctgttcg cgcttttacg ccagccacct ggaaactgaa
     3541 ggattgctgg gcagaggatg gcggctgaac tgggagataa acctgcgtga ggatgaaaca
     3601 tatatcacct tcatcggtgt acagggtcgg gaactgagtt atccgaaaga gatgctgata
     3661 ccgggccacc agatattcga cccggaagag cagttttacc tgagccgtct gcatgacgga
     3721 tgctatgtgc tgcattacac cgaccgcagc tattacgtgt ttgatgaatt tgatgaccac
     3781 ggcgtcgcgc cgttactttt tatggagacg ccgtaccggc agcgtattgc tttcgggcgt
     3841 gagaacggca ggctggtgcg ggtggcctcc agcagcggac accatctgtt attgcaccgc
     3901 acgatgacgc aagcagggga acgcctgtcg catattgaac ttgtgaaggg cgggcgtccc
     3961 ggtaacctgg tggaatatcg ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg
     4021 gctggtgtga cggtgcgcca gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac
     4081 gcgacgggct ttacctgtac ctaccactgg gaagagattg aaggtttccc gcgcgtggtg
     4141 gaacacacga ccagcgacgg cgaggattac cggttccact atgattttgc tggcgggcag
     4201 acggtggtaa ccggcaggcc ggagcagaag tggcagtggt ggtttgatga agagacgtat
     4261 gtgactgcgc accggacgcc gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat
     4321 tttccggtag cggtggaact ccccggcgaa cgcagggtaa cgctggaata cgacaccctg
     4381 agccgggtgg tgaaagagac ggacccggca ggccgggtga cacagacgca gtggaacggc
     4441 agttttgctg aaatcacgcg ccgggcgctg gatgacgacc acgtatggaa ggcggattac
     4501 aacgaacacg gccaggtgat acgggagacg gacccggaag ggcgcgtcac gcggtacggg
     4561 tatgatgaac aggggctggc ggtcagccgc acggatgcgc ggggcggcga ggttgcgctg
     4621 gcgcacgatg cgcgcggtca actgaggcgt tacaccgact gttcagggtg cgccacgggt
     4681 tatgaatacg atgaagacgg caacctgacg gcggtgacgg acgcggaggg gaaaacggta
     4741 cgcatcagct ataaccgtca ggggctgccg gagacggtct gccaccccgg taaacagcag
     4801 gaccgttaca cctggaacgc gctggggtta ctgagcagcc accgtcgcat cacgggcagt
     4861 gtgcagtcct ggcagtatac gccgcgcggg atgctggcgc gccatacgga tgaagagaaa
     4921 cgggagacgc gctggcagta caccccggaa gggctggttg ccagtctgag caacggcaac
     4981 ggggctcagt accggttcag ctacgacggc gacggacggc tgacgggaga acagcgcccg
     5041 gacggcctga taaggatgtt tgcgctgaat gcggacgggt ttccggtcat catccagacg
     5101 cagggcacag agggcggggt gcgcaatgaa cagcaggagc gtgatgcgct gggccgcctg
     5161 ctgcgcagtg acacgcagca cagtacacgg accttcagct acaaccgtct tgaccagata
     5221 acggaagtga cgctcacccc cacggaagaa ggggaacggc tgcaccacat gcaggcggac
     5281 acggtgcgtt ttgcgtatga ccgcagcggc tggctgacca gccgcacggg gcagatgtat
     5341 gaccatgacc attattatta cgacaaagcg ggcaacctgc tgacggatga gtatcaggga
     5401 gcggtgatgg ataaccgcct gccgggctac gggcgtgacc gttaccgcta taacgaatgg
     5461 ggcgaactga cagagcgcag ggaccagcaa ctggagtgga acgcgcaggg ccagttaacg
     5521 cgggtcatca gcagcaacag cgatacgctg tatcagtatg atgcgctggg caggcgcatc
     5581 agcaaggtga catatggccg ccatcacggg gcgcggagag agccagaaaa tctggtatta
     5641 ccacacggac ctgacgggca cggtacacga ggtaaccgcg ccggacggga cgctggtgtg
     5701 ggcgggctac caggcgggat ttggtgaaaa ccggggcgac atcagcaaca gcggggcgta
     5761 ctttgagcag ccacagcggc tgccggggca gtactttgac gaagaaacag ggctgcatta
     5821 caatctgttc aggtattatg cgccggagtg cgggaggttc gtaagccagg acccgatagg
     5881 attaaacggt gggttgaact tatatgctta tgcgcctaat ccgttaggtt ggatcgatcc
     5941 gctaggactg aattctttag gaatagattc aaataatata tttagaggag at
//
LOCUS       DADMFL010000179         5930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.183, whole genome shotgun sequence.
ACCESSION   DADMFL010000179 DADMFL010000000
VERSION     DADMFL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.183"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..109)
                     /locus_tag="JNZ27_003838"
     CDS             complement(<1..109)
                     /locus_tag="JNZ27_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HAY8665972.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIG"
     gene            complement(366..698)
                     /locus_tag="JNZ27_003839"
     CDS             complement(366..698)
                     /locus_tag="JNZ27_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY8665973.1"
                     /translation="MEQIQPLKIIKGGAEPGVWGVELLAIRYAAWIKPEFEIEVYEVF
                     KTVVRLGVGAMSRLNRIDHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEV
                     QMYLPGMV"
     gene            complement(671..871)
                     /locus_tag="JNZ27_003840"
     CDS             complement(671..871)
                     /locus_tag="JNZ27_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY8665974.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1077..1229)
                     /locus_tag="JNZ27_003841"
     CDS             complement(1077..1229)
                     /locus_tag="JNZ27_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665975.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1217..1684)
                     /locus_tag="JNZ27_003842"
     CDS             complement(1217..1684)
                     /locus_tag="JNZ27_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8665976.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1681..2157)
                     /locus_tag="JNZ27_003843"
     CDS             complement(1681..2157)
                     /locus_tag="JNZ27_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY8665977.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2141..2464)
                     /locus_tag="JNZ27_003844"
     CDS             complement(2141..2464)
                     /locus_tag="JNZ27_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY8665978.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2689..2907
                     /locus_tag="JNZ27_003845"
     CDS             2689..2907
                     /locus_tag="JNZ27_003845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665979.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3176..3799)
                     /locus_tag="JNZ27_003846"
     CDS             complement(3176..3799)
                     /locus_tag="JNZ27_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8665980.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASGA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3796..3984)
                     /locus_tag="JNZ27_003847"
     CDS             complement(3796..3984)
                     /locus_tag="JNZ27_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY8665981.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3981..4343)
                     /locus_tag="JNZ27_003848"
     CDS             complement(3981..4343)
                     /locus_tag="JNZ27_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8665982.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4340..4630)
                     /locus_tag="JNZ27_003849"
     CDS             complement(4340..4630)
                     /locus_tag="JNZ27_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8665983.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4630..5352)
                     /locus_tag="JNZ27_003850"
     CDS             complement(4630..5352)
                     /locus_tag="JNZ27_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8665984.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5345..5554)
                     /locus_tag="JNZ27_003851"
     CDS             complement(5345..5554)
                     /locus_tag="JNZ27_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HAY8665985.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            complement(5514..5915)
                     /locus_tag="JNZ27_003852"
     CDS             complement(5514..5915)
                     /locus_tag="JNZ27_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665986.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT         1348 a         1521 c         1343 g         1718 t
ORIGIN      
        1 tgccaatgaa ctgaaatgct tcttctgcgg ctgcgttctg gttcataacc agtttgtaaa
       61 tctctaactg gaatttctgt tcttcagtca tgggaataat ctctgccatt gttggctccg
      121 tttatccgtt aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcga
      181 gaccactcat tgaatggcct ctgcaataac cgatgtcttt ccatcagtcc gccaccacaa
      241 agaatctttt ttgccataag gctggaggtt catctttcag tggctgccag tgttatttcc
      301 ccacttactg gcttgggttg tttcgcggta ctgccgtaat gcaaaaactg gattaacctg
      361 cgaaatcaca ccattccggg caaatacatt tgcacttcat ttgccgctct ctcacgtgca
      421 acatgaagca atctttttcg cccaccaacg ccccacttag ccatttggct tgcgcactgg
      481 cttatcgctt tggtttcagt attgatgatg tgatcgattc tattcagacg ggacattgcg
      541 ccaacgccga gacggacaac cgttttgaaa acttcataaa cttcgatttc aaattccggc
      601 ttaatccatg ctgcatatct gattgccaga agttcaacac cccacacacc tggttctgca
      661 ccacctttga ttattttaag tggttgaatt tgttccaaag tgcttttatg aagcgtttta
      721 tctgcgcgct acgcaaaaac tggcttgggc gctgttgctc tgtagcctct ccatttgcaa
      781 ctgctgctgc atggagatcg tttaagttgt agcgtccatc ctcatcaaca cgaacggaca
      841 caccattgac aataactgtt gggtacttca tcagtgatta ccttttagtg atgaaccttg
      901 tcacacagga ttccggccca cagaaaggca ccgattacca aaccggcatc ctcaagggtc
      961 atcctgaaag gctctgtgtt cataagtcgc gcgtgtgaag cgcgtttgtt gcagatataa
     1021 aaaagccccg cgaatgcgag gctaaatcct ggtgtttgtg atgactggct cttatctcaa
     1081 cgcagcccct taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaat
     1141 ctggatttac tccagaagtg atcaccaccc tgtctacaga gccagatgtg aaggatgatg
     1201 agtaaaatta tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca
     1261 gtgctgtttg gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg
     1321 gagagagttc gacggtggca tcattgccca tgccggaggc gctggaggtt tcggctgagg
     1381 atggcacagg gcattttcct ttgacgagca ccctgccacc attatcaagc ttacgccgaa
     1441 gagcatcatt ttcagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat
     1501 cagcagcacg ctggcgttgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac
     1561 tgactttttt atctcgctgc tctttgtagg tcatggcgtt atcacggtaa tgattaaccg
     1621 cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc
     1681 tcatacatca atctctctga ccgttccgcc tgcttctttg aattttgcaa tcaggctgtc
     1741 agccttatgc tcgaactgac cataaccagc gcccggaagt gaagcccaga tattgctgca
     1801 acggtcaatt gcctgacgaa tatcaccgcg atcaatcatc ggtaaagcgc cacgttcttt
     1861 aatctgttgc agtgccacag cgtcctggct tttcggagag aagtctttca ggccaagctg
     1921 cttacggtaa gcatcccacc aacgggaaag aagctgataa cgtccggcgg ctgttgattt
     1981 tagttttggg tttagtgtga caagtttgcg aggatgatcg gagtaatcag taaatagctc
     2041 tccgcctaca atgacgtcat aaccatgatt tctggttttc tgacgtccgt tatcagttcc
     2101 ctctgaccac gccagcatat cgaggaacgc cttacgttga ttattgattt ccaccatctt
     2161 ctactccggc ttttttagca gcgaagcgtt tgataagcga accaatcgag tcagtaccga
     2221 tgtagccgat gaacacgctc gttatataag cgagattgct acttagtccg gcgaagtcga
     2281 gaaggtcacg aatgaaccag gcgatgatgg cgcacatcgt tgcgtcgatt actgtttttg
     2341 taaacgcacc gccattatat ctgccgcgaa ggtacgccat tgcaaacgca aggattgccc
     2401 cgatgccttg ttcctttgcc gcgagaatgg cggctaacag gtcatgtttt tctggcatct
     2461 tcatgtctta cccccaataa ggggatttgc tctatttaat taggaataag gtcgattact
     2521 gatagaacaa atccaggcta ctgtgtttag taatcagatt tgttcgtgac cgatatgcac
     2581 gggcaaaacg gcattaggtt gttagcgcaa cctcatgccg cccactttca cgaagatcat
     2641 gtgtagaagg ccgcagcgta actatcactg atgaattcag gatagtcagt ggctacggct
     2701 cagtttggat tgtggcgacc ggtgctgatc tccggtttgc tgcaactgcc tacagctggc
     2761 tacgtggcca caccgaatcc agcgaaagat tcttgccctt acacatcagc ctgtgcattc
     2821 accacaacgg aaagagcact ggctaaccag gctcgccgac tcttcacgat tatcggctca
     2881 atgctcttac ctgttgtgca gatataaaaa atcccgaaac cgttatgcag gctctaacta
     2941 ttacctgcga actgtttcgg gattgcattt tgcagacctc tcagcctgcg atggttggag
     3001 ttccagacga tacgtcgaag tgaccaacta ggcggaatcg gtagtaagcg ccgcctcttt
     3061 tcatctcact accacaacga gcgaattaac ccatcgttgg gtcaaattta cccaacttta
     3121 ttcaataagt caatatcatg ccgttaatat gttgccatcc gtggcaatca tgctgctaac
     3181 gtgtgaccgc attcaaaata ttgtctgcga ttgactcttc cttgtggcat tgcaccacca
     3241 gagcgtcata cagcggctta acagtgcgtg accatgtggg ttgggtaagg ttggggatta
     3301 gcatcgttac agcgcgatat gcgccgcttg ctggcatcct tgaatagccg acgcctttgc
     3361 atcttccaca ctctttctca acaactctcc cccacagctc tgttttggca atatcaaccg
     3421 cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtcgcggca ctacggcaat
     3481 aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta tttccttcaa
     3541 gctttgcaac gccacggtat ttccccgata ccttgtgtgc aaattgcatc agatagttga
     3601 tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaatgca gccattccga
     3661 atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg gaaagagagt
     3721 cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa tgaaatttag
     3781 ctacgctttc gagtctcatc gtcttcccct cttgccctgt ttgaccatca ggacgccgtt
     3841 aactattacg tgacgctcgc ctttgctgtc tcggttgtac ttgagcactg ttcctcttgc
     3901 gcaggaaagc atcctcgcca cttcggtctg attgcctcgt gtctggataa gaagctctgg
     3961 tatcgtttga attgtggcgt tcatacgttc tccagttcgg tgatttttat tccaagcctt
     4021 ccgcctggta ctttcacacc acgaattacg cgaatgtcat cgaattgctc gtcgtcttcc
     4081 gcaaatccgg cgtggataag ggagtcgagt aaacctttca ggatgttatc gaggtcgcgg
     4141 cggcgggagt ctggaacgtc tgcgatgact ttgatgcgga gtcgtgattt ggtgaaaatg
     4201 tctaacttga gttggaggat tatttgctga acttcttttc ggtatttctg gcctttatcg
     4261 ctgatgtagt attggtttcc ccgtcttcgc cagtaggtgt tcaccgacgg cgggtatgga
     4321 agcacaaact gatattcgct catgacttaa tcttcccctc cttcagcagt atcgcctgcg
     4381 tcctgatcac gccttcgagg tggtaaagtc tggcgtcttt gttgtcgaga ttatgggtgc
     4441 gtcggtcgat ttcatcgtga cacgcgctac aagcccatgc gccgatcagg tcgtcaggct
     4501 tcattcccgt tccgcaaatt ccagccatcc ggtaatgtgc cagaactgta gtttcaggat
     4561 taccattgca tacgccgtaa atacgtacct ggcattctct gccgcgtgct tctttgcgta
     4621 gattagccat taagcagcct cccctgttac tttcagcatt ccattatcga gcagctttct
     4681 ggtcagccac tgttgaccac gcccggtgat ttttgtggtg aacgatatct gtattccgtg
     4741 atttgtgttg accgctgttt ctttcactgt gaaatagccg cgatccatat attcctgcat
     4801 tggcacattg cgccgggaac ctgaagcaat aaggattttg tgatcgcgca tccacgcaaa
     4861 cagtttgttt ggaccaattc caacaacctt tgcaaagttt ccaatcaaaa ttccgctggc
     4921 ctcgccaacg cgatcggcaa actcaacttt aggtgcggca attgcgagct ggttttccag
     4981 ttgcattttc tgctcagcaa gatcagcagc aaggcgcaac gcttccggta gcgttttggg
     5041 gatattaacc gcagcttctt caagctctcg ccagcggtca acaaggcgag cggtgaattc
     5101 cggcgacaac tgggctacaa cgacaatact gtctcgcttt ccttgttcgc cttcgaacac
     5161 atacacacaa aaactttgat ttaagcctaa cccattgatt cttccacaat cctcaatttg
     5221 aggaagccgg ataacaccat ttttagccag cgtttcgatg gtacgtttca cattgtcatg
     5281 acgcttacca accaactcag cgatttcaat gcttgtcatt ttgatggcat tgccatttat
     5341 taactcattc atcgtcttct tcctcgtaca ttgagctatt cggatcgctc atcagttctg
     5401 cgcagcagtg ctcacacacg tgaacttcca gcacatgcag cttctgaccg caattagcgc
     5461 acgttaaagc tcgctcgacg ctttctttct ggtattgaag ggattgggat gggctaagca
     5521 tggctttcac cattaaaaag tcgcttgtaa gcatcgatgt ctcgttttgc ttcaccgagc
     5581 tttcgtctta attccatgtt ttctgattca agcttttcca tgtcttgttg gtatcgatcg
     5641 cggtgttctt tccatgcttt tcgatacgcc ttcatgtatg tcgtattggc ctttctcttt
     5701 gcctgacgaa ctgcgtggtg gtttttcaca aaccagtcag ggtcgttaaa tgctgctctg
     5761 gcgcaggtat accaataatt tgttgcctcc ctgtttagcc aataaatact gataaatggc
     5821 aactgtatcg acaccatttt tcgttgagac tctttctcgc caaacatgtg cccttttttg
     5881 atgctaaggc caaatccagg ttgaattaaa agcattgtca tttcctcgca
//
LOCUS       DADMFL010000180         5887 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.184, whole genome shotgun sequence.
ACCESSION   DADMFL010000180 DADMFL010000000
VERSION     DADMFL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.184"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            145..663
                     /gene="mddA"
                     /locus_tag="JNZ27_003853"
     CDS             145..663
                     /gene="mddA"
                     /locus_tag="JNZ27_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY8665987.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            660..1109
                     /locus_tag="JNZ27_003854"
     CDS             660..1109
                     /locus_tag="JNZ27_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8665988.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1110..1342)
                     /locus_tag="JNZ27_003855"
                     /pseudo
     CDS             complement(1110..1342)
                     /locus_tag="JNZ27_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1428..1601)
                     /locus_tag="JNZ27_003856"
     CDS             complement(1428..1601)
                     /locus_tag="JNZ27_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY8665989.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1796..1891
                     /gene="yncL"
                     /locus_tag="JNZ27_003857"
     CDS             1796..1891
                     /gene="yncL"
                     /locus_tag="JNZ27_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY8665990.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2293..3102)
                     /locus_tag="JNZ27_003858"
     CDS             complement(2293..3102)
                     /locus_tag="JNZ27_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY8665991.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3312..4737)
                     /gene="patD"
                     /locus_tag="JNZ27_003859"
                     /pseudo
     CDS             complement(3312..4737)
                     /gene="patD"
                     /locus_tag="JNZ27_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4759..5553)
                     /locus_tag="JNZ27_003860"
     CDS             complement(4759..5553)
                     /locus_tag="JNZ27_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8665992.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALVTLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5543..>5887)
                     /locus_tag="JNZ27_003861"
     CDS             complement(5543..>5887)
                     /locus_tag="JNZ27_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8665993.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1550 a         1485 c         1304 g         1548 t
ORIGIN      
        1 ctagtctaaa aactagtatt aagactatca cttaaataag tgatactggt tgtctggaga
       61 ttcagggggc cagtctatcc gttgcttcgg gctgttttcg atagtattga taacatcatc
      121 atcattattt tattgaggcc agacatgtcc atccgttttg cccgcaaagc cgactgtgct
      181 gccattgcgg aaatttataa ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa
      241 acggtggatg cagataaccg cattgcctgg tttgaagcgc ggaccatagc aggttatccg
      301 gtgctggtaa gtgaagaaga tggcgtagtt acgggatatg cctcgtttgg cgactggcgt
      361 agtttcgatg gttttcgcca taccgtggaa cattcggttt atgtccatcc cgatcatcag
      421 ggcaaaggtc tggggcgtaa attgttaagc cgattgattg atgaagcgcg ggattgcggg
      481 aagcatgtca tggtcgccgg gatcgaatcg caaaatcagg cttcgctgca tctgcaccag
      541 tcgctgggat ttgtcgtcac cgcacaaatg ccgcaggtag gcactaaatt tggtcgttgg
      601 ctggatctga catttatgca gttgcaactc gacgagcgca ctgaaccgga cgcgattgga
      661 tgaatcagtc gctcaccctt gcttttttga ttgccgccgg aattggtctg gtagtccaga
      721 acacgctgat ggtgcgcatc acccagacct cctctaccat tctcatcgcc atgttgctga
      781 actcactggt ggggattgtg ctctttgttt ctattttatg gtttaaacaa ggcatggcgg
      841 ggtttggcga actggtgtcc agcgtgcgct ggtggacact tatacctggc ttgctgggtt
      901 cgttttttgt cttcgccagt atcagcggat atcagaatgt gggagcggcg acaactattg
      961 cggtactggt cgccagccag ctgattggcg gtctggtgct ggatatcttc cgcagtcatg
     1021 gcgtaccact acgggcgttg ttcggcccaa tatgtggcgc aattttactt gtggttggcg
     1081 cctggttggt ggccagacgc tcattttaat cagaggatgg tgccgccttt ggttaactgt
     1141 tcgtggccgc ttccatatct tctttatact tgcgaccgtg atgagcaata gcggtgcgca
     1201 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     1261 ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     1321 tgacttcatc cagatgcgac atactttctc cttagccact tgtgaaattt atcgattaat
     1381 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     1441 aacaacagcg ccaccgggaa actcatcggc catgtggccc ccaccagaaa ggcgcttaag
     1501 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     1561 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccata
     1621 tgaataaaac gcgcgcaaat atgcgccatt ttttgacgtg catcaagttt tttcaccagt
     1681 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     1741 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     1801 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     1861 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     1921 tcagagaaat caactctctg acagtacgca agatcttctc atactctcca agtttgcaac
     1981 attatcctta atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc
     2041 cccctactgt cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt
     2101 ttctatcacg aatactttat acgcgcggga gtctataaat gctttatact ttcaaggaat
     2161 aacgtaaata taaaaagatg gcgtaaagta tatgcatggc aggatgtttg caacctcctt
     2221 gatgacgctg ccccattgaa attgcaaacc ccgccctggt tatcttgccc atgtatcatg
     2281 caaaacagat aattataggc ctctgaatac ctcagaaaga ttgaattttt tatagtccct
     2341 ctcttgaata tttccatttg atatcaatcg attcatctct tcaacaaaat aatttatatc
     2401 atcacacttg ttattatccg gaagatctga gtagttataa ttccaccatt ttattctcaa
     2461 cagttcatca ataacatttg aatcaaacct aaatctgatt atttttgcag gaactcctgc
     2521 aactatggca tatgggggca catctttggt aacaacggaa ttggcagcaa taacagcacc
     2581 atcacctatt gcgatgccac ctttcaatac aacatcattt cctatccaga catcatttcc
     2641 tataatcgga ttttcaggtt tggtaatgaa tggtttcaat gtccagctac gttcaaattc
     2701 actcatagca aacttatcaa attcaccgtt ataagtcatc atgtgagtag taaaacgatt
     2761 caaaggatga tttccaccca ttatttttac atttgatgca atactacaaa atcgacctat
     2821 acgtatattc cctgaaaaat ggcaggtcgg aaatgaaaaa gcgccgacac tggagaaatt
     2881 tttcacaggc attgttgaat attcttcagc aataacatta tcttcaattg ttatcttgtc
     2941 accttttgag aaaagaaata aattcctttc tttatacgac ttcttcccct tatgatttaa
     3001 aaagatttta ttatccatac aaaactcaaa gtgttttttc gtccatctga aggtaagggt
     3061 tttattttta tcttccgtga atttgtacaa ttgaactttc attgttaata cttctactta
     3121 taaattatta gtttaataac gatactctaa agccttatgc taatcaactg tgacaggatg
     3181 aaaaatcgcc atcaaacatt tagcctaatt tttacgccag ttattaatgt aatcattcta
     3241 atacttaacc gccattaaac tcagaaccta aaacgatagt ggccgtccta taacagccac
     3301 taacatctaa cttaatgttt aaccatgacg tggcggacga cggtgtaatc ctccagccca
     3361 taaagtgaca tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg catttcactt
     3421 accagcatga aatgggtatt gacccaggta caaccatatt gcagccgtgc gctgacgcga
     3481 tgcgccctgc ccacatcttt cgtccatacc gaagatgcaa gtccgtactg gctgtcattc
     3541 gcccagttca ccacctgttc ttcgttgtcg aagggcgtaa cactcactac tggaccaaat
     3601 acctcttttt gcacaatggc atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag
     3661 taatagccat tacccttgcg cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct
     3721 ttcgcctctt ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca
     3781 agctccgtag actcgtcatc cggcgcacca gattttaacg ttgccaccgc aggcacccag
     3841 tttttccacc agcgtatcgt aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca
     3901 atcctgtcca gcattgtaat agccaaatgt acgtacacct tcgaccactg cttcaatatc
     3961 cgcatcatca aaaacaatca ctggcgcttt gccaccaagt tccatatgag tacgcttaat
     4021 ggacgacgcg gtatggctga tgatgtgctc gccggtggcg atagagccca tcagcgacac
     4081 catccgcact ttgggatgac cggtcagcgg atcacccacc gttttgcctc tgccaaacag
     4141 tatgttaatc acgcctgccg ggaagatatc tttcgccagc tctgccaact tcaacgcggt
     4201 cagcggggta atttctgatg gtttaagcac tacgcagttc cctgccgcca gcgccggagc
     4261 aagtttccac gcggccatca tcagcggata attccacggt gcgatagaag ccacgacccc
     4321 caacggatcg cgacggatca tcgaagtatg accttcaaaa tattcacctg ccgccagacc
     4381 attcagacag cgcgccgcac ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc
     4441 atcattgaac gcactatgca gcggtttgcc acaattacgg gattccagtt cggcaaaaac
     4501 ctgaccattt tcttcgataa catccgccag tttcagcagg cattccgcac gcgctttcgg
     4561 tgtggtttgc ccccattcag caaatgctgc atctgccgca cgcacagcag catcgacctg
     4621 ctctgcggat gcctcggcaa tctccagtaa aacgtccccc gttgccggat tatagacagg
     4681 ctgtttttcc ccttcgccgc taaccagttc tccgttaatc agtaacttat gttgcataga
     4741 ttattcctgt atcagtggtt attgaccatt gtcgccttcg cgggttagcc accaggcccc
     4801 caggatcggc aaggttgtta ccaacataac cagcagtgcc accacgttgg ttaccggtac
     4861 atcacgcggt cgcccaagct gattgagcaa ccacaacggt aacgttcgtt catgacctgc
     4921 cgtaaaggtc gtaacgatga tttcatcgaa cgacaaggca aacgccagca ttcctcctgc
     4981 cagtaacgcc gaactgagat tcggcaacac tacgtagcgg aaggtttgcc agccattggc
     5041 cccaagatcc attgacgcct caaccagact ccacgaggtg cggcgaaaac gggcgatgac
     5101 attgttaaac accaccacta cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc
     5161 cggctccaga ttgatggttt taaaggcggt taatagcgcc agaccagtga caatgcccgg
     5221 cagcgcaatg ggcagcagca ataacagcga aatggcgttt ttgccgaaaa agtctcgtct
     5281 ccacagcgcg gcagctgcca gcgtccctaa caccagcgca attaatgtca ccagcgccgc
     5341 cactttaagt gacagcgtca cggcatcaag aatatcacta cgctgtgctg ccacgctaaa
     5401 ccagcgcagc gtcaggccct gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc
     5461 ataggcggcg attatcagga tgggaaaatg taggaaaaca acgccgcccc aggccgccag
     5521 tttgaggaaa aacggtgcgc gttcagagtg catcgaacgc tcccagacgt ttcacgaacg
     5581 ccaggtacag tgcgatgaga ataatcggca ccagggtgaa tgccgccgcc atcggcatat
     5641 tgccaatcgc cccctgctgg gaataaacca tattgccgat aaaatatcct ggaggaccaa
     5701 ccagctgcgg gacgataaaa tcgcctagtg tgagtgagaa ggtaaagata gagccagcgg
     5761 caatacccgg gattgccagc ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc
     5821 cgagatcagc cgacgcctgc aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg
     5881 gcaggat
//
LOCUS       DADMFL010000181         5824 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.185, whole genome shotgun sequence.
ACCESSION   DADMFL010000181 DADMFL010000000
VERSION     DADMFL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.185"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..173
                     /locus_tag="JNZ27_003862"
                     /pseudo
     CDS             1..173
                     /locus_tag="JNZ27_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(230..649)
                     /locus_tag="JNZ27_003863"
     CDS             complement(230..649)
                     /locus_tag="JNZ27_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665994.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILASSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            699..1877
                     /gene="ltrA"
                     /locus_tag="JNZ27_003864"
     CDS             699..1877
                     /gene="ltrA"
                     /locus_tag="JNZ27_003864"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY8665995.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWFILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVTTSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1960..2139
                     /locus_tag="JNZ27_003865"
                     /pseudo
     CDS             1960..2139
                     /locus_tag="JNZ27_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2169..2651)
                     /locus_tag="JNZ27_003866"
                     /pseudo
     CDS             complement(2169..2651)
                     /locus_tag="JNZ27_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2907..3887)
                     /locus_tag="JNZ27_003867"
     CDS             complement(2907..3887)
                     /locus_tag="JNZ27_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY8665996.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3887..5086)
                     /locus_tag="JNZ27_003868"
     CDS             complement(3887..5086)
                     /locus_tag="JNZ27_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY8665997.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5408..5746)
                     /locus_tag="JNZ27_003869"
                     /pseudo
     CDS             complement(5408..5746)
                     /locus_tag="JNZ27_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1595 a         1335 c         1383 g         1511 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 ctgaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgta atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg ttcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgt aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtca caacgagttt cacctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1561 cgtcaaatgc agcaatgcgc aaaataacag aaacaatcaa gaagtggcgt atacatcgct
     1621 caacagctga gagtttgctg gattttgcga ggcgctacaa tgcgatagtg agaggctgga
     1681 tcgagtacta cggaaagttc tggtccagaa atttcaacta tcgactgtgg agtgcaatgc
     1741 agtcacgtct gctcaagtgg atgcagtcta aatacagact ttcgaaccgg aaggctcagc
     1801 gaaagctgac gctggtaagg aaggagtatc cgaagctatt tgttcactgg tatttactac
     1861 gtgcatcaaa tgagtgatca agagccgtat gacgcgagag tgtcacgtac ggttccgtga
     1921 gcagccgggg agtgaaattc tctccggctg actcgactcg cgggttccga cagcgatgac
     1981 gaacatgcgg cggtgttgta ctcgctgatc ggcacatgcc gtctgaacaa tgtggaacca
     2041 gaaaaatggc tgcgttacgt cattgagcat atccaggact ggccggcaaa ccgggtacgt
     2101 gatctgttgc cctggaaagt tgatctgagc tctcagtaaa tatcaatacg gttctgacga
     2161 gccgcttact tttggcgtaa ttctgctgcg cctttactga acacaatgcc gtattcagca
     2221 agaagtccac gaatgtgatt tatcagttgt gtcctttcca ttgaaaccaa ccgacgcata
     2281 ttcaaaaaag tctggacgtc ctgttgctcc agagttttta ttggtacggg tcgtatagtt
     2341 ccatgttggc aggcctcagc tattgcctgt gcgtcgttat aatcattttt ctggcaccgc
     2401 tgataagctt taacatgttg tgccggaagc aatattacat catggtcgag tttcctgatt
     2461 tcccgggccc agtaatgaga tgccccgcca gcctccatcg caacagtcat tttaggctgt
     2521 tgcacctgcc agttaagcag ttgattgcga gaaagtttgg tctttcctgc gggtttgcca
     2581 tgagcattga tactgaaaag ataaaagttc gttttagcca gatcaatgcc ggtgattttc
     2641 tgttcagaca tggtatcccg cttacttttg tggttaaagt ttatccatca tggcgcaatt
     2701 gaaggctgcc gtgaaagagg gggaagacta tctcatcggt tactgttaaa cggctaagag
     2761 aaactatctt tttcaagcaa agaagatacg gtgaaacagg cgatttcacc gtgaaatact
     2821 gttcaaaaaa aacgctaata aaagaaaaat ttcttttaaa acagccaatt aaaaactggt
     2881 gagttgctgg cgaaaaactt gaattttcac agcgaattca gattatcttt tagtacaaga
     2941 gcaatcatac gatctaactt atcctgaact tccaacggta atctgccgaa ttcatatgag
     3001 aacgtacgtc cctttactcg ttttcgagca aatttatctt tactatcaaa tttccataac
     3061 aaagttacaa ccgctttatc ctttactcct gaatcaagca atgaagacat ttccttggtt
     3121 ataagccgca taatactgtt tttaacctcg tcttcagaaa gattgtcatc agcagtaata
     3181 agctcaatac tcggcgagat attcttcaca acttcatcaa tagatatatc acccagcctc
     3241 agtcgttcac ttaattcagc cagttgcctg taatcggcgt atgtcagctc agactgaaca
     3301 ggaaaaagag acacaaaatc tttaggtacg gacgctgctt ggagggcacg agtcacctta
     3361 gctgccgaca atccctctct ttccgcaatt tgcttctggt tcataccggc ctctttgaga
     3421 cgtactaaac gcaacccaat ttcacgaata ttatgttcaa gtgacgtctg caaatctttc
     3481 gccagatgct gagcttcaga aacagtaagc tcatcatccg ttacaagtac acgtaatcca
     3541 accttgcata gcaaagctgc tgcccgacga cgggaaccat ccaaaatctc aataagatcg
     3601 ccagtccgaa tcccaataca tggatagaat tgttgtaaac gtatagttct ggtgatgtct
     3661 tttagtgagt ccggcgtcag ggctgtctgt tcgcggccat tcacctcttc aaccacaaaa
     3721 gtttgagtgt ccactttgtc atgatcaacc ttgatctcag taaattttgc ctttcggcca
     3781 gtattgaggg taaatacatg aactggagcc gatatttctt cagtattatt caggatattt
     3841 gtattcagcg tacggccaat agtcggtcgg tgctttctgt tttccatcag tagttggccc
     3901 ttataaattc aatacgatca aaaactgctt tagcgaaatc ttctgctgcc atacgtgcat
     3961 ttttcaaagc ttcaccactg ccaacataag taacaggatt cgcagatatc acggtatcaa
     4021 acgactcgcc gcttctctca aaaccatcaa gacgtggcat tgaaacatca agcatgtcac
     4081 caccgaaaat ctcttttgtc aggctatggc agtatttatg atctgactta ttcgccagtt
     4141 tcgacatgaa gccaatattt gcctgcaaac ggcatgaaca tccatcctgt tcaataatct
     4201 gtacaagttc aggtaaacga gccaaatatt taagcgtgga atggaaatca acttgtgctg
     4261 gcggcaccgg tgtaaacatg atatctgcgg cagcaatggc atttttcaaa aatgcgtcca
     4321 gatgcgggcc tgtatcaata aggatgaagt cgaaatcatg cttcagctta tcaataatat
     4381 tctcacgcaa tatcgcgtgt ttgttttgtc ctaatagatg ctcctcgcac agagtatccc
     4441 agtttgatgc aataaaggcg tcgtcaatag aagccggcat aacataaacg ccagggataa
     4501 ctgacggaac gataaaatct tccagtaatt cctcacgact cacgttctgc aacatagcct
     4561 gcggtgcggt tgtatcaacc agccccaccg catgcaagta gttcagaaac atagtggctg
     4621 atgactgggg atctaaatca aggagcagaa tacgtaaatc ttctgacagc aggtgaggat
     4681 gagcacgcag ggcgtgagct acggaaacac tactcacggt tttagacaca ccgcctttta
     4741 gactaccgat gaatatggaa tatgcctcac tataacgatc gcgatattta ggaatgccac
     4801 gatgtgcata gatatcgata atgttctgga tggttaatgc atattttgtc gcggttcctg
     4861 ctgggcgttt ttcaaactga tagccctgag cctccatctc accgactgca ccatccacgc
     4921 ttgcgcgcgt taattttgga agcttagcta cagcagactt tgaatagact tgataaaaaa
     4981 catcagcctg aaactcctgc ttttgctcct gaatctgttt tgttagggcg agcaacattt
     5041 tatctgcacg ctgcgcaacc ttacttaact gctcaaaact agtcattccc cctccgtgct
     5101 atgtaggatt ttaacttatt acaaaaaaaa ctgcattagc agggtaatgc atgcaagtac
     5161 atattgtaca tgcttatatt aaaaatgcgc gcattgctga tttattaatc atcggaatga
     5221 atattactgt aagatagtat gcctttccgg gagaaagacg ctgatttccc ccgaataatc
     5281 cagttcacca ccacactccg gacaacagct ttcttcaggc tccagatggt gtatttcgcg
     5341 ggaaacatgt tccggtaacg gacggcgatg acgtgactgt cgcaacaggc gggggtagtg
     5401 cttccaatca gtagaacatg tgcttttcaa tgaaggagcc gatgactttt tcgtggattt
     5461 ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg gtgcggaggg
     5521 tccggttgtt acgttcaata cgttgcgtga agattttgcc ggtcaggtgt ttctccttcg
     5581 gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag
     5641 tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
     5701 acacaccgcc agttttagtg ttgtacgcat accagagcca gtgttgtgtc aacgacggat
     5761 gaaaagtgct ccacttatat ctccaccaac ggcccaatat tgatccaccg ttttactcag
     5821 gatt
//
LOCUS       DADMFL010000182         5784 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.187, whole genome shotgun sequence.
ACCESSION   DADMFL010000182 DADMFL010000000
VERSION     DADMFL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.187"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..121)
                     /locus_tag="JNZ27_003870"
     CDS             complement(<1..121)
                     /locus_tag="JNZ27_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8665998.1"
                     /translation="MLKKVKRRLYKEGRYSCQLPKCDTTKWSVDDWCNWIDRYG"
     gene            complement(130..462)
                     /locus_tag="JNZ27_003871"
     CDS             complement(130..462)
                     /locus_tag="JNZ27_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8665999.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNKRTWYSKPRGEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(923..1327)
                     /locus_tag="JNZ27_003872"
     CDS             complement(923..1327)
                     /locus_tag="JNZ27_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666000.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(1324..1956)
                     /locus_tag="JNZ27_003873"
     CDS             complement(1324..1956)
                     /locus_tag="JNZ27_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY8666001.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPMNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            2060..2275
                     /locus_tag="JNZ27_003874"
     CDS             2060..2275
                     /locus_tag="JNZ27_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8666002.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            2395..2688
                     /locus_tag="JNZ27_003875"
     CDS             2395..2688
                     /locus_tag="JNZ27_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666003.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            2721..2867
                     /locus_tag="JNZ27_003876"
     CDS             2721..2867
                     /locus_tag="JNZ27_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 domain-containing protein"
                     /protein_id="HAY8666004.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            2860..3693
                     /locus_tag="JNZ27_003877"
     CDS             2860..3693
                     /locus_tag="JNZ27_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAY8666005.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            3683..5119
                     /locus_tag="JNZ27_003878"
     CDS             3683..5119
                     /locus_tag="JNZ27_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8666006.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEINLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            5196..5636
                     /locus_tag="JNZ27_003879"
     CDS             5196..5636
                     /locus_tag="JNZ27_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY8666007.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRPVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            5633..>5784
                     /locus_tag="JNZ27_003880"
     CDS             5633..>5784
                     /locus_tag="JNZ27_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY8666008.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIR"
BASE COUNT         1597 a         1347 c         1388 g         1452 t
ORIGIN      
        1 ttccgtatct atctatccag ttacaccaat catcgacact ccattttgtt gtgtcgcatt
       61 ttggcaactg gcaagaatat ctaccttctt tgtaaagtcg gcgtttgact ttcttgagca
      121 tggctcacct caatcgtaat aagctggaat tgattttccg cgttgcttct ggcggcctga
      181 gcaagtcaca cctatttcac cgcgtggctt gctgtaccat gtgcgcttat tcttgcgctc
      241 aatacgctgc aggttgcttt caatctgttc gtggtattca gccagcaccg taaggtctat
      301 cggattcagt gcgctttcta ctcgtgattt cggtttgcga ttcagcgaga gaatagggcg
      361 gttaactggt tttgcgctta ccccaactaa caggggattt gctgctttcc attgagcctg
      421 tttctctgcg cgacgttcgc ggcggcgtgt ttgtgcatcc atctggattc tcctgtcagt
      481 tagctttggt ggtgtggtgg ctggtagtct agctccagct tgttgagtct cattcggagg
      541 ggtataaccg gcaccccagc gatttttcca tgcgacaacg tgcgcgttat ggcggcctta
      601 tcgcccgcgt ctccccatct cgtccacgct attgctagcg ttgggagcgc ttcaccgctc
      661 aacagtaggt aagcacttgc cagtgactag ctggcttcac cacaccccaa agccttctgc
      721 tttgaatgct gcccttcttc agggcttatt ttttaagagc ctcaccttca tggtggtcag
      781 tgcgtcctgc tgatggctta aaattacaag aaagattgta tgttgcaaac aagaaatatt
      841 gtgaaaaggg gcgtgaaaaa caaactccat tgtttttaaa tgaaaaatag tttgtttttg
      901 gttatcgaga ttgaggtggg gattactggt tgcaggttcc gactacatca ccaacaaatg
      961 atttggttga tgtaagttgt tgcatacctg ggatattcat tactttggag taaagagctt
     1021 ttttgtctgt agtgattgac caagtttcaa cggttattcc tcctccagac tggtattctc
     1081 ctaccatagt gttcgatgac aaagcagtgt atttcatctc tggatagacg ccagaaactg
     1141 attcataaac tgatgattta tcgccattta ttgttacgtg gaaaacggaa tcttccgtgc
     1201 tgtcttttgt aaactcgtaa cgatcgccat tcattgaccc gtacccgtgc aggtttgtga
     1261 caatccagca ttcagaattg gcgctggtag ttaagagtat tgagagtagc gccgcaatcc
     1321 tgatcatacg aattttaccc tcgcttccac gacaacaccg ataatcttgc agttcccgtt
     1381 gataggagtc ataggccatg aagggttcag gcctttcagg tacttctgcc cgccatctat
     1441 aaccagtttc ttgaatgttg cttcgttcgc gtcagtcagt ttggctacaa caaggcttcc
     1501 attcattggc tcgcgtccag tatctactaa caccatatga ccttcaggga tgctttgacc
     1561 tacaggtgag gtcatggaat caccttcaac cttcagccag aatccatcgc ctaataagtt
     1621 aacgtcactg tcataccatt catcaatgtc cttgatatcg tagggttcac aagcttcaca
     1681 ccacgaacca gctctaacca tgctaatcaa tggatatttc cctttgggtt caacatgccc
     1741 aacaaatcta acattcgaat cagaggtgcc attgagcagc cagtcaacac ttacgccaag
     1801 agctgacgca agttctggta aaaagcgtgg tcgcttagtt ttaccgtttt cgagctgctc
     1861 tatagactgc tgggtagtcc ccaccttttg agcaagttca gcctggttaa gtccaagctg
     1921 aattcttttg ctttttaccc tggaagaaat actcataagc cacctctgtt atttacctcc
     1981 aatcttcaca agaaaaactg tatttgacaa acaagataca ttgtatgaaa atacaagaaa
     2041 gtttgttgat ggaggcgata tgcaaactct ttctgaacgc ctcaagaaga ggcgaattgc
     2101 gttaaaaatg acgcaaaccg aactggcaac caaagccggt gttaaacagc aatcaattca
     2161 actgattgaa gctggagtaa ccaagcgacc gcgcttcttg tttgagattg ctatggcgct
     2221 taactgtgat ccggtttggt tacagtacgg aactaaacgc ggaaaagccg cttaagacat
     2281 tcccgctctt acacatccca gtcctgaaaa agggcatcaa attaaaccac acctatggtg
     2341 tatgcattta tttgcatacc ttcaatcaat tgttatctaa ggaaatactt acatatggtt
     2401 cgtgcaaaca aacgcaacga ggctctacga atcgagagtg cgttgcttaa caaaatcgca
     2461 atgcttggaa ctgagaagac agcggaagct gtgggagttg ataagtcgca gatcagcagg
     2521 tggaagaggg actggattcc aaagttctca atgctgcttg ctgttcttga atggggtgtc
     2581 gttgacgacg acatggctcg attggcacga caagttgctt cgattctcac caataaaaaa
     2641 cgcccggctg caaccgagcg ttctgaacaa atccagatgg aattctgagg tcattactgg
     2701 atcaatccac aggagtaatt atgacaaaac aactcagtcc ttaccaggac aaaattcaca
     2761 aacacatact acgtgatcgc ttcctgtcca gcttcaagca gcctggtcga ttccgggctg
     2821 agttggaaaa agtgaagctg atgcagaagg agaaaggtca tgagtaacat atctaatcta
     2881 gccgaagcca gagaggccag aaggctacaa caaccgcatc aaagcagcgg taaggggtat
     2941 gccttgctgc accgtaaaat tatggatgtg ccgttttaca aggacgcaga agcagcgcat
     3001 ctgtgggttc acttaatcct caaagcaaag catacgcctg agtatgtaat gactgacgca
     3061 ggagaaattc tggtaggcag agggaagcta cttggcggta gaaactctct ggcgtttgaa
     3121 acaggactca aaccagatcg cgttcagtac ctgcttagaa agttcaaaaa actcggcatg
     3181 attgactggg tttcacacgg taaattctca gttttctcgg tagagaaata tgacgattat
     3241 cagtcaaatt ttgtaccagc agattaccag caaattacca cagataaaga atataataat
     3301 attatctcta atactgacgt attagagagt accgcagcag acaaaaagtc tgacaagaaa
     3361 aaaccttccg ttagctgtca ggatgttgtc gatgcttacc acgaaatcct tcctgaagcg
     3421 ccaaaaatcc gcgcactgaa tgacaagcgt aaaaaccaga tccgaacgtt ctggcgcaaa
     3481 gccggagtga taacccgcca gcttgacggg catgggttca cgatgcagga ctggagaaat
     3541 tatttgagct acgtaggcga aaattgccga tggatgttcg aagagcgccc aaaccatcag
     3601 cgcggaaccg tctggcacaa aaagggattt gatttcctgc ttaacgataa tacctacctg
     3661 aaagttcgtg agggtgaaca cgatgaccga taatttttat gcgccgcccc atagcatcga
     3721 ggcagagcag gcggtgattg gtggattgct tctggatgat gacagcagtg agcgcgtcca
     3781 gaaggttctg gcgatgctga agcctgattc attttacagc cgaccacaca aaatcatttt
     3841 cgaagaaata accagaatgc accgggagca aaagccagta gatggcctga cgcttttcga
     3901 tgaactggag cgtaaatcgt taacggcgtc tgttggcggt tttgcttata tcgctgagat
     3961 cgcaaagaac acgccaagcg ccgcaaacat cgttgcctat gcaatgcagg ttcgcgaaac
     4021 cgcaatggaa cgctacgcca tcaaccgcat gactgaagcg acggaattgc tctattcccg
     4081 caacggaatg actgcgacgc agaagtacga agctattcag gcgattttca cgcaactgac
     4141 agaccatgca aaaacaggat cgcgtcgcgg ccttcgctca tttggtgagg tcatggaaga
     4201 ctgggttagc gaccttgaga agcgatttga cccatcaggc gaacaacggg gaatgagcac
     4261 agggatccca tcgctggaca ggatgctgtc accgaaaggt ctggtgaaag gctctctgtt
     4321 tgtcattggc gctcgaccta agatggggaa aacgacgcta tacagccaga tggcaatcaa
     4381 ctgcgcagtg catgagaaaa agcccgctct tatgttcagc cttgaaatgc caggtgacca
     4441 gatactggaa aaactggtag ggcagaagtc tggtgttaac ccgaatattt tttaccttcc
     4501 ggcgacaaat gacgctgatg acggctatca gggtgattac gatggtgact tcaacagggc
     4561 gatcgaaaca gccaatcgct tgagtgaaat caacctgctt tacatcgacg acacgccggg
     4621 attatctctg gctcaaatcg tcagcgaaag ccgtcgaatc aagcgagaaa aaggatgtgt
     4681 tggcatgatt ctggtcgatt acctgacact aatgaccgct gaaaaggccg atcgcaacga
     4741 ccttgcttac ggcatgatca ccaaaggact gaagaacctt gccaaagagc ttgattgcgt
     4801 tgttgtgctt ctgacacagc ttaaccgcgc actggaaagc cgaaccaata aacgcccatt
     4861 accaagtgac tcacgagata cagggcagat tgaacaggat tgcgattatt gggtggggat
     4921 ccatcgtgaa ggtgcttttg atgacagtgt tccacctagt gaaaccgaac taatccttcg
     4981 tctcaatcgt catggcaata ccggcacggt gtattgcatt caggcaaatg gcgctattta
     5041 tgacacagac caacagtctg ctgaaatgcg ccgacgtgaa cgcgaggaac cgcagtccaa
     5101 gaagaaagga ggattctgat gaccatctac atcactgagc taataacagg tctgctggta
     5161 atcgcaggcc tttttatttg ggggagaggg aagtcatgaa aaagctaacc tttgaaattc
     5221 gatctccagc acatcagcaa aacgctattc acgcagtaca gcaaatcctt ccagacccaa
     5281 ccaaaccaat cgtagtaacc attcaggaac gcaaccgcag cttagaccag aatcgaaagc
     5341 tttgggcttg ccttggtgac gtctctcgtc cggttgaatg gcatggtcgc tggctggatg
     5401 cagaaagctg gaagtgtgtg tttaccgcag ccttaaagca gcaggacgtt gttcctaacc
     5461 ttgccgggaa tggctttgtg gtaataggcc agtcaaccag caggatgcga gtaagcgagt
     5521 ttgcggagct attagagctt atacaggcat tcggtacaga acgtggcgtt aagtggtcag
     5581 acgaagcgcg actggctctg gagtggaaag caagatgggg agacagggcg gcatgaggcg
     5641 acagcgacga agtttcaccg acatcatctg cgaaaactgc aaataccttc caacgaaacg
     5701 ctccagaaat aaacgcaagc caatcccaaa agaatctgac gtaaaaacct tcaattacac
     5761 ggctcacctg tgggatatcc ggtg
//
LOCUS       DADMFL010000183         5741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.188, whole genome shotgun sequence.
ACCESSION   DADMFL010000183 DADMFL010000000
VERSION     DADMFL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.188"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            58..1517
                     /locus_tag="JNZ27_003881"
                     /pseudo
     CDS             58..1517
                     /locus_tag="JNZ27_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1553..1756)
                     /gene="ydfZ"
                     /locus_tag="JNZ27_003882"
     CDS             complement(1553..1756)
                     /gene="ydfZ"
                     /locus_tag="JNZ27_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY8666009.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1934..2620)
                     /locus_tag="JNZ27_003883"
     CDS             complement(1934..2620)
                     /locus_tag="JNZ27_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8666010.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENSLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2709..3455)
                     /gene="ydfG"
                     /locus_tag="JNZ27_003884"
     CDS             complement(2709..3455)
                     /gene="ydfG"
                     /locus_tag="JNZ27_003884"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY8666011.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3592..5637
                     /gene="dcp"
                     /locus_tag="JNZ27_003885"
     CDS             3592..5637
                     /gene="dcp"
                     /locus_tag="JNZ27_003885"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY8666012.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1436 a         1362 c         1487 g         1456 t
ORIGIN      
        1 atttttattc atataacaaa acatatagct tgccatattt aaatttaagg aattgttatg
       61 ggaaataatt tgttatcagc aaaagcgaca ctccctgttt atgatcgtaa taacctggcc
      121 ccaagaattg ttcatttagg ctttggtgca tttcaccgtg cgcatcaggg cgtgtatgcc
      181 gatattcttg ctacagaaca tttcagtgac tggggatatt atgaggtcaa cttaatcggc
      241 ggcgaacagc aaattgccga tttacaacag caagataatc tttataccgt tgcggaaatg
      301 tcggccgatg cgtggacggc tcgcgtcgtt ggcgtcgtta aaaaagcctt gcacgtacag
      361 atggatggct tagaaaccgt gttggctgcg atgtgtgaac cgcaaatcgc gattgtctct
      421 ctgacaatca ccgaaaaggg tatttccact ctccggcgac cggacagtta atgctcgatc
      481 acccgatggt cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca acaggggtga
      541 ttgttgaggc gctggctcgc cgtaaagcgg caggacttcc cgcatttacc gtcatgtcat
      601 gtgacaacat gccagaaaac ggtcatgtta tgcgtgacgt tgtcacttcc tacgcgcaag
      661 ccgttgatgt aaaactggca caatggatcg aagataacgt gactttccca tcaacaatgg
      721 tggaccgtat tgtgcccgca gtggcagagg atacgctggc gaaaatcgaa caacttaccg
      781 gtgtgcgcga tcctgctggc gttgcctgtg aacctttccg ccagtgggtt atcgaagata
      841 actttgtagc cggacgtccg gaatgggaaa aagcgggagc cgaactggtt agtgatgtgc
      901 tgccttatga agagatgaag ttgcgcatgc tcaacggcag tcattcattc ctggcatatc
      961 tgggttatct tgctggatat cagcacatta atgactgtat ggaagatgaa cattatcgtc
     1021 atgcggcgta tggcatgatg ttgcaggaac aagcgccgac gctgaaagtg cagggcgttg
     1081 atttgcaaga ttacgctaac cgattaattg cacgctatag caacccggcg ttacgtcatc
     1141 gaacctggca gattgcgatg gatggcagcc agaaattgcc acagcggatg ttggattctg
     1201 ttcgctggca tctggcgcat gacagcaagt tcgatctgct ggcgctgggc gtcgcgggtt
     1261 ggatgcgtta tgtcggtggt gttgatgaac agggaaatcc gatagaaatc agtgacccac
     1321 tgttacctgt tattcagaag gctgtacaaa gtagtgccga agggaaagcg cgcgtccagt
     1381 cattgctggc gattaaggca atctttggtg atgatttgcc agacaatagc ttgtttactg
     1441 caaaagtgac ggaagcgtac ttgtctttat tagcgcatgg cgcgaaagcg accgtggcga
     1501 aatattccgt gaagtaaaaa aagcaatgcc gccatcggcg gcattcacac acttagttca
     1561 tgccgagacg aatcaggtcc agaggtgcca gtttctcttc acaaccttca acaactacgg
     1621 tttttccgcg gcggatttgc tcagcggtca gaccttcagt atcaatcgac aggatcttgc
     1681 cagtgtgacc ggtgccgcta accataacac ggctgccagt ggtgattgcg ttacggttac
     1741 gatcgtaggt cgtcataggt gttttctcct ttctgattta cagtcgaagc ccacgtaacg
     1801 ggcgcaatat aaatactcct gtcgagtgaa atattttttg tttttgatca agctcacatc
     1861 ttttttgatg catgcatgag ccgtaaggcg gggggaaaag cctctcatcc cggatgggat
     1921 gagagtaaat gaattactct tcgctaaacc agttgctgtt ttcctggcgg atctggcgca
     1981 cggattcgct gatttcctgc aaatgttgcg tcattgcacg ttctaccgca tcgccatcac
     2041 gtttttgcag ggccgagaaa atatcaagat gctggcgtaa cagcatttct ggtggagaaa
     2101 catggtcgaa actcatatag cgcacgcgat caacggtcgc tttcaggttc tcaatggtat
     2161 cccacgccag ttgacagtcg gcaatctgcg tcaggagttg atggaagttg tcatcaagtt
     2221 caaaaaaatc atccagttgc ttgcgctcaa tggcaatgcg ttgctggtga agattttgtt
     2281 ccagttggta gcactggctt tcggtaatca tgctcgccgc ccgacgcgcc accgcgcact
     2341 caatggcctg acggataaaa ctgccgttgc gcacctgggc catggaaatt ttgttgacgt
     2401 agctgccacg ctgcggacga atttgaatca ggctgttttc cgccagttta ataaaggctt
     2461 cacgaaccgg ctggcgtgac acattgaaac gaacagaaac ttctttttcc gacaacggtg
     2521 tgcctggtgc aatcaggcaa tggacaatgt cgcgacgaag aatacgataa atctgctgat
     2581 tgacaggctg tgtgggatta agttgcgttt cgacggtcat tcgttcttgc ttgtgagtga
     2641 gttaactgca tgagtctacc acttttttgt gacaagcaat aacccggcag ttacgccggg
     2701 tataaaaatt actgacggtg gacattcagt ccggcatagc tttgggtaac cggcatcatt
     2761 tccagggtat tgatattgac gtgagcaggc agcgttgaca cccaccagac ggcttcgctg
     2821 acatcttctg gcgtcaatgc aacggtattt tgataggttt tttccgcttt accgtcatcg
     2881 cctttaaagc ggacattgga aaactcggtg ccacccacca gacccggttc gatgtcggtg
     2941 acgcgcaccg ccgtaccatg cagatccgta cgcagattca ggctaaactg acgaacaaac
     3001 gctttcgtcg caccgtaaac gttaccaccg gcatacggcc agctgcctgc cgttgagcca
     3061 atgttaataa tatgaccatg attacgttca accatacccg gtaagacggc gcgcgtcata
     3121 tatactaggc ctttgttgtt ggtatcaatc atcgtttccc agtcttcaac gctggcttta
     3181 tgcgcaggct ccatgcccaa cgccaggccg gcattattta ccaggatatc aatattgctc
     3241 cactcggcag ggagcgatgc cagcatctct tcaatagcgg cgcggttgcg aacgtccagt
     3301 tgggcgatat acagattatc tcccagttcg tcttttaact cctgcaaccg ctcctggcga
     3361 cggccagtgg cgataacttt atgcccttgt tgaataaaac gacgagtaat gcattcacca
     3421 aaacctgccg ttgctccagt tactaaaacg atcatctcac tgttcctcaa cgcttttgtg
     3481 tgtcaataac atagcacgtg atgcttaaga gcgggtaata ctggtttgca agattgcact
     3541 ccgttatcag taagcctacg ctatggaaca cctcgcgttc aggagaagaa aatgacaaca
     3601 atgaatcctt tccttgtgca aagcacactg ccgtatctgg ctcctcattt tgatcaaatt
     3661 gccaatcatc actatcgccc ggcattcgat gagggaatgc aacaaaagcg ggcagaaatt
     3721 gctgccatcg cgcttaaccc gcaaacgcct gatttcaaca atactattct ggcactggaa
     3781 caaagcggag aattaattac ccgcgttacc agcgtctttt ttgcgatgac tgcggcgcat
     3841 accaatgatg aattacagcg tcttgatgaa cagttttccg ctgaactggc ggaactggct
     3901 aatgatatct atctgaacgg tgaattattc gcgcgggtag atgctgtctg gcagcgccgt
     3961 gaatccctgg ggcttgatag tgaatccatc cgcctggtgg aggttattca tcaacgtttt
     4021 gtccttgccg gagccaaact tgcacaagct gataaagcaa aattaaaagt actgaataca
     4081 gaagctgcga ccctgaccag ccagtttaac cagcggttac ttgcagcaaa taaatccggc
     4141 ggtctggttg tgaacgatat cgcgcagctg gcaggaatga gtgagcaaga gattgcgctg
     4201 gcggcagagg cggctcgcga gaaaggtctg gataacaaat ggctgattcc gctgctgaat
     4261 accacccaac aaccggcgct tgccgaaatg cgcgatcgtg cgacgcgtga aaaactgttt
     4321 attgcgggct ggacgcgagc ggaaaaaaat gatgccaatg atacccgcgc tatcattcaa
     4381 cgtctggtag agattcgcgc acagcaggcg aaactgcttg gttttcctca ttatgccgca
     4441 tggaaaatcg ccgatcagat ggcaaaaacg ccagaagcag cactcaactt tatgcgggaa
     4501 attgttccag cggcgcgtca acgtgcgagc gatgaattag cctccataca ggcggttatc
     4561 gataagcagc agggcgggtt tagcgcgcag ccgtgggact gggcatttta tgcggaacag
     4621 gtacggcggg agaaatttga tcttgatgag gcgcagctca agccatattt tgaattaaac
     4681 acggtgttaa atgaaggtgt attctggacc gcgaatcagc tcttcggtat taagtttgtc
     4741 gaacgttttg atattccggt ctaccatcct gacgttcgtg tgtgggaaat ttttgatcat
     4801 aatggcgtgg gactggcgtt attttacggt gatttcttcg cccgtgattc aaaaagcggc
     4861 ggtgcatgga tgggcaattt tgttgagcaa tcaacgctta atgaaacgca tccggtaatt
     4921 tataacgtct gcaattatca gaaacccgct gccggtgagc ctgcgttgtt actctgggat
     4981 gatgtaataa ccttattcca tgaatttggt catacgctgc acggcctttt tgcccgccag
     5041 cgttatgcca cgctttccgg caccaacacg ccgcgtgatt ttgtcgaatt tccgtcgcaa
     5101 atcaacgaac actgggcaac gcatccgcag gtattcgctc gctacgcccg gcattatcag
     5161 agcggggcag caatgcctga cgaactgcaa cagaaaatgc gtaatgccag cctgttcaac
     5221 aaagggtatg agatgagcga actgcttagc gccgcacttc tcgatatgcg ctggcattgc
     5281 ctggaagaaa acgaagcaat gcaggatgtc gatgattttg aattgcgggc gctggtggcg
     5341 gaaaatatgg atcttcctgc tataccgcca cgctatcgca gcagttattt cgcccatatt
     5401 tttggtggcg gatatgctgc aggttattac gcttatctgt ggacgcaaat gttggccgat
     5461 gatggttacc agtggtttgt tgagcagggc ggattaacgc gtgaaaatgg gcagcgtttt
     5521 cgcgaggcga tcctttccag aggtaacagc gaagatctgg aacgcctgca tcgacaatgg
     5581 cgcggtaagg ctcctcagat tatgccgatg ctgcaacatc gtggcttgaa catataaaca
     5641 ccaccaatgg ctggcctgga gatatcgcca ggccagcctg gttatagcag taattgcagc
     5701 atgttacgac aatgctccga tggaaagctt ctggggaacg c
//
LOCUS       DADMFL010000184         5711 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.190, whole genome shotgun sequence.
ACCESSION   DADMFL010000184 DADMFL010000000
VERSION     DADMFL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.190"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            34..435
                     /locus_tag="JNZ27_003886"
     CDS             34..435
                     /locus_tag="JNZ27_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY8666013.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JNZ27_003887"
     CDS             447..818
                     /locus_tag="JNZ27_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666014.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JNZ27_003888"
     CDS             805..1395
                     /locus_tag="JNZ27_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666015.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JNZ27_003889"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JNZ27_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JNZ27_003890"
     CDS             1802..2542
                     /locus_tag="JNZ27_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666016.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JNZ27_003891"
     CDS             2601..2987
                     /locus_tag="JNZ27_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY8666017.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JNZ27_003892"
     CDS             2996..3325
                     /locus_tag="JNZ27_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY8666018.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..5711
                     /locus_tag="JNZ27_003893"
                     /pseudo
     CDS             3297..5711
                     /locus_tag="JNZ27_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         1414 a         1318 c         1816 g         1163 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacag tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcggca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc ggagggctga
     5641 ggaggtgctg aaggggagcc tgaagcgaca actgaatgaa aacaaacgct actggcagga
     5701 actggaagtg g
//
LOCUS       DADMFL010000185         5638 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.191, whole genome shotgun sequence.
ACCESSION   DADMFL010000185 DADMFL010000000
VERSION     DADMFL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.191"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            84..299
                     /locus_tag="JNZ27_003894"
     CDS             84..299
                     /locus_tag="JNZ27_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8666019.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            292..678
                     /locus_tag="JNZ27_003895"
     CDS             292..678
                     /locus_tag="JNZ27_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY8666020.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            686..1372
                     /locus_tag="JNZ27_003896"
     CDS             686..1372
                     /locus_tag="JNZ27_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8666021.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1350..1973)
                     /gene="tetR(B)"
                     /locus_tag="JNZ27_003897"
     CDS             complement(1350..1973)
                     /gene="tetR(B)"
                     /locus_tag="JNZ27_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY8666022.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2055..3260
                     /gene="tet(B)"
                     /locus_tag="JNZ27_003898"
     CDS             2055..3260
                     /gene="tet(B)"
                     /locus_tag="JNZ27_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY8666023.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3373..3966)
                     /gene="tetC"
                     /locus_tag="JNZ27_003899"
     CDS             complement(3373..3966)
                     /gene="tetC"
                     /locus_tag="JNZ27_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY8666024.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4054..4464
                     /locus_tag="JNZ27_003900"
                     /pseudo
     CDS             4054..4464
                     /locus_tag="JNZ27_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4480..>5638)
                     /locus_tag="JNZ27_003901"
     CDS             complement(4480..>5638)
                     /locus_tag="JNZ27_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY8666025.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1562 a         1041 c         1225 g         1810 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccgaccac
       61 tattaagtca tgtagctggt tttatttcaa ttgaacgttt tcaaagtcta gctacagaag
      121 gaaaaatgtt atcgctatct tggtgggaaa acgaatacgc agttctgcaa tggaaaaatc
      181 atgttttaca tgcgaaagct caacaagaag ggcgagagtc aatatttgat ttttacaaaa
      241 ttagtattgc tcatattact cgcgaatatt catttaaaaa ggacaaggat aatgtttgat
      301 gttcacgttg ttttagataa tcaaatagga caattagcat tactaggaaa aacattaggt
      361 aataaaggta ttggattgga agggggaggg atatttacgg ttggtgatga atgccatgct
      421 cattttcttg ttgaacaagg aaaggaagct aaaatagcgc tagagcaagc tggactgtta
      481 gtacttgcga tccggacacc attaattcgt aagttaaaac aggaaaaacc gggggaactt
      541 ggcgaaatag cacgagtatt ggcggagaat aacattaata ttttagtgca atacagtgac
      601 catgctaacc aactgatatt aataacggac aatgatagta tggctgcatc tgttacgctc
      661 ccttgggcaa taaagtgaac ttgcgatggc taatttaata cgaaaagagg ttacctttga
      721 gtcctcaata gccgcgatag gggcggctat gtctgacatt tcacgagtta aaatactcag
      781 tgctttgatg gatgggcgag cttggacggc cactgagcta agttctgtgg cgaatatatc
      841 agcttcaacg gcgagcagtc atttatctaa attattagat tgccagctaa tcacagtagt
      901 agctcaaggc aagcatcgtt attttcggct agcaggaaaa gatattgctg aattgatgga
      961 aagtatgatg gggatctcct taaaccatgg cgtacatgcc aaagtttcca cgccagtgca
     1021 tttacgaaaa gcacgtactt gctatgatca tttagctggc gaagttgccg ttaagatcta
     1081 tgattccctt tgtcaacagc aatggatcac tgaaaatggt tcaatgatca cattaagtgg
     1141 tattcaatat tttcatgaaa tgggaattga cgttccttcc aaacattcac gtaaaatctg
     1201 ttgtgcgtgt ttagattgga gtgaacgccg tttccattta ggtgggtacg ttggagccgc
     1261 attattttcg ctttatgaat ctaaagggtg gttaactcga catcttggtt accgtgaagt
     1321 taccatcacg gaaaaaggtt atgctgcttt taagacccac tttcacattt aagttgtttt
     1381 tctaatccgc atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga
     1441 tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc
     1501 ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc
     1561 cccacagcgc tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag
     1621 gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact
     1681 tttgctccat cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa
     1741 aaatcttgcc agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc
     1801 tcaatggcta aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc
     1861 tgctctacac ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca
     1921 ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga catcattaat
     1981 tcctaatttt tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga
     2041 tagagaaaag tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg
     2101 ccatggggat tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt
     2161 cggaagatat cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta
     2221 tctttgctcc ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt
     2281 tgtcattaat aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga
     2341 tgctgtattt aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat
     2401 cggtcattgc cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg
     2461 caagttttgg gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt
     2521 caccgcatag tccctttttt atcgctgcgt tgctaaatat tgtcgctttc cttgtggtta
     2581 tgttttggtt ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg
     2641 agacgcaatc gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta
     2701 tttatttttc agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg
     2761 aaaatcgttt tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt
     2821 tacactcagt attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa
     2881 cggcagtact gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat
     2941 ctgaaggttg gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac
     3001 ctgcattaca gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg
     3061 gattattggt gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta
     3121 tttataatca ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt
     3181 actgtattat tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta
     3241 aacaggagac aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat
     3301 gaccctcttg ataacccaag agggcatttt ttacgataaa gaagatttag cttcaaataa
     3361 aacctatcta ttttatttat ctttcaagct caataaaaag ccgcggtaaa tagcaataaa
     3421 ttggcctttt ttatcggcaa gctcttttag gtttttcgca tgtattgcga tatgcataaa
     3481 ccagccattg agtaagtttt taagcacatc atcatcataa gctttaagtt ggttctcttg
     3541 gatcaatttg ctgacaatgg cgtttacctt accagtaatg tattcaaggc taattttttc
     3601 aagttcattc caaccaatga taggcatcac ttcttggata gggataaggt ttttattatt
     3661 atcaataata taatcaagat aatgttcaaa tatactttct aaggcagacc aaccatttgt
     3721 taaatcagtt tttgttgtga tgtaggcatc aatcataatt aattgctgct tataacaggc
     3781 actgagtaat tgttttttat ttttaaagtg atgataaaag gcacctttgg tcaccaacgc
     3841 ttttcccgag atctcatcta ttgaaacagc ttgatagcct ttttcaacaa acaatattcg
     3901 tgctgagtta accagtgatt gataggtact cttaaaattt tcttgttgat gatttttatt
     3961 ttccatgata gatttaaaat aacataccgt cagtatgttt atggtatcat gatgatgtgg
     4021 tcgtgacaat cttaagaaca tttaggttat tttatgtata ttgaacagca ttctcgctat
     4081 caaaataaag ctaataacat ccaattaaga tatgatgata agcagtttca tacaacggtt
     4141 atcaaagatg ttctattatg gattgaacat aatttagatc agtctttact gcttgatgat
     4201 gtggcgaata aagcgggtta taccaagtgg tattttcagc ggctgttcaa aaaagtaaca
     4261 ggggtcacac tggctagcta tattcgtgct cgtcgtttga cgaaagcggc tgttgagttg
     4321 aggttgacga aaaaaactat ccttgagatc gcattaaaat atcaatttga ttcccaacaa
     4381 tcttttacac gtcgatttaa gtacattttt aaggttacac caagttatta tcggcgtaat
     4441 aaattatggg aattggaggc aatgcactga gagatcccct cataatttcc ccaaagcgta
     4501 accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt cttcccttgt
     4561 tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg agagtacgtt
     4621 tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct gagcatgaac
     4681 gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga tatcaaaacg
     4741 ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac ttttcaagtc
     4801 tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt gtttgggtgt
     4861 tcgaatttca acaggtaagt tagttgctag aacccatggc tcctttgccg acgctgagta
     4921 gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt ttcggccttt
     4981 agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag tcagcctctt
     5041 atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga taggtttcca
     5101 gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta accagtacca
     5161 acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac tgacaatgag
     5221 cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt catgagcttt
     5281 ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag aacgaccgtg
     5341 tagtgcgact gaagctcgca ataccataag tcgtttttgc tcacgaatat cagaccagtc
     5401 aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc aacggtatac
     5461 agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc gtttgatgtt
     5521 atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag tgagagtttt
     5581 acagtcaagt aatgcgtggc aagccaacgt taagctgttg agtcgtttta agtgtaat
//
LOCUS       DADMFL010000186         5576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.192, whole genome shotgun sequence.
ACCESSION   DADMFL010000186 DADMFL010000000
VERSION     DADMFL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.192"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..87)
                     /locus_tag="JNZ27_003902"
     CDS             complement(<1..87)
                     /locus_tag="JNZ27_003902"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8666026.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNZ27_003903"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNZ27_003903"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY8666027.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JNZ27_003904"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JNZ27_003904"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY8666028.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1729 a         1256 c          935 g         1656 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgcc cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact gaaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5461 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
     5521 gttctggaaa gtcagggcga atataactca caatgggcag cgatttgttc cattgc
//
LOCUS       DADMFL010000187         5574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.193, whole genome shotgun sequence.
ACCESSION   DADMFL010000187 DADMFL010000000
VERSION     DADMFL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.193"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..166
                     /locus_tag="JNZ27_003905"
     CDS             <1..166
                     /locus_tag="JNZ27_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666029.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="JNZ27_003906"
     CDS             349..2529
                     /locus_tag="JNZ27_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY8666030.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="JNZ27_003907"
     CDS             complement(2670..3953)
                     /locus_tag="JNZ27_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY8666031.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="JNZ27_003908"
     CDS             complement(4088..5158)
                     /locus_tag="JNZ27_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8666032.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..5354)
                     /locus_tag="JNZ27_003909"
     CDS             complement(5136..5354)
                     /locus_tag="JNZ27_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8666033.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         1321 a         1369 c         1402 g         1482 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc attaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaatatcct gcactcgcgt
     5281 acccatcgac gaactgtttc aaggctcctt gggcgtcgct ggcgtgcgtt ccactcctga
     5341 agtgtcaagt acatcgcaaa gtctccgcaa ttactcgcaa gggagcgagt attggtttat
     5401 tttctccaac aaaaaaggag ccgaagctcc tttgatgatt aaaattcgaa ttgtctcgcc
     5461 cgaaggcttt tcagcattgg ttttgcccgt tcgaaaaggg cgcttgcttg gtcaagtcgt
     5521 gtcgcctccc ttagcagtac atctctgttt ttcgtcaccg tgtaaaaagt ttca
//
LOCUS       DADMFL010000188         5553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.194, whole genome shotgun sequence.
ACCESSION   DADMFL010000188 DADMFL010000000
VERSION     DADMFL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.194"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..638
                     /locus_tag="JNZ27_003910"
     CDS             <1..638
                     /locus_tag="JNZ27_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666034.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JNZ27_003911"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JNZ27_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY8666035.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="JNZ27_003912"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="JNZ27_003912"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY8666036.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1374 a         1358 c         1541 g         1280 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggtctgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac atcggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgcgtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac ggctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cagcatttcc tta
//
LOCUS       DADMFL010000189         5547 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.195, whole genome shotgun sequence.
ACCESSION   DADMFL010000189 DADMFL010000000
VERSION     DADMFL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.195"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JNZ27_003913"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JNZ27_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY8666037.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JNZ27_003914"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JNZ27_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY8666038.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELHQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5547)
                     /locus_tag="JNZ27_003915"
                     /pseudo
     CDS             complement(2659..5547)
                     /locus_tag="JNZ27_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1467 a         1288 c         1305 g         1487 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaattttac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acatcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt atagatattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gatgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagccag
     4921 tttgatggcg atcccggcag caggtttgcc gttatagcgc gccacggtgg aataatcttc
     4981 cgccccaagt tcgacgcggg cgacatcacg cagcagcact tgcgaaccat cttgctgaac
     5041 tttcaacagg attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga
     5101 ggcgtttagt tgctggtctg ccacccagtt gaccaccaga aatctggttg ttttgcacct
     5161 taatctggga aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca
     5221 gccagatacg catagcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac
     5281 ggcttagcgg gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc
     5341 tgccgttatc agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc
     5401 taatcccctg ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt
     5461 gcacctgaac ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg
     5521 cattacccgc cgcatcactg gttgaag
//
LOCUS       DADMFL010000190         5531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.196, whole genome shotgun sequence.
ACCESSION   DADMFL010000190 DADMFL010000000
VERSION     DADMFL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.196"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            101..1009
                     /locus_tag="JNZ27_003916"
     CDS             101..1009
                     /locus_tag="JNZ27_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY8666039.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1055..3046)
                     /gene="tkt"
                     /locus_tag="JNZ27_003917"
     CDS             complement(1055..3046)
                     /gene="tkt"
                     /locus_tag="JNZ27_003917"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8666040.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3324..4082
                     /locus_tag="JNZ27_003918"
     CDS             3324..4082
                     /locus_tag="JNZ27_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8666041.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDGHPASAERAQHIRDRISADGIK"
     gene            complement(4288..5208)
                     /gene="speB"
                     /locus_tag="JNZ27_003919"
     CDS             complement(4288..5208)
                     /gene="speB"
                     /locus_tag="JNZ27_003919"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY8666042.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JNZ27_003920"
     CDS             complement(5343..>5531)
                     /locus_tag="JNZ27_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666043.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1322 a         1484 c         1478 g         1247 t
ORIGIN      
        1 tttcgctata cccctttata gattctgaat tctcacctat cggctgtttt ctcctcatct
       61 catgtgacat attcccatac tttactcaag gaagattgtc atgaaagcac ctgtttcatt
      121 aacctcattt cgcccacaaa aatctcttgc tatcgccatt ggcgtgcttg ccgtggttgt
      181 gctgccattt ctctcgtatt acaccgttaa tgaaggtgaa cgcggcattt tgctgcgcta
      241 cggaaaaatc gtaaaagttg ccgagccggg gctgggtttt aagatccctt ttatggaaag
      301 cgtggagaaa atttccacgc gtaatcaggc tgtggtctat cagggattac aggcctacag
      361 ccgcgaccag caaccggcac aaatgacagt atcagtcagc ttccacatta aaccttcaga
      421 agcaggcgcg gtttatacca cctacaacac tattgaagca ttaaaagatc gtctaattgt
      481 ccgtcagttg ccaactcagc tggagaacat tttcggccaa tataccgcca tcagcgccgt
      541 acaggatcgt acaaaattgg ttcaggatct gcaaaatgcc atgcgcaaag ccgttgttgg
      601 gccagtggta attgacgggg tccagattga aaatattgat ttttctgacg cttatgaaaa
      661 gtcgattgaa aaccgaatga aagcagaagt cgccattgcc acgcgtaagc aaaatctgga
      721 aacagagaaa atccaggcgc aaatagcggt aactcaggcc caggcagaag cagacagtaa
      781 actggcggca gccaaagcag aagcggaaac tattcgcgta agaggcgcgg cagaggctga
      841 aactattcgt ttaaaaagtg cggctgaagc agaagcaatc cgcctgcgcg gtgaagctct
      901 gcgggataat cccggacttg tagccctcac cactgccgaa cgttgggacg gtaaactgcc
      961 cgataccatg atccccggca gcacagttcc gtttatctct actaagtaaa aaaagggccg
     1021 ctttcgcggc ccttctcaca aaacgtctta ctgattacag cagcgctttt gctttcgcaa
     1081 caacgttatc aacagtgaag ccgaactctt caaacagcag ctctgccgga gcagattcac
     1141 cgaaggtggt catgccgacg atagcgccgt tcaggccaac atacttgtac cagtagtcag
     1201 caatacccgc ttctaccgca acgcgtgcag taaccgcttt cggcagtacg gattcacggt
     1261 aagccgcatc ctgcttgtca aatgcgtcgg tagacggcat ggacaccacg cgcgctttca
     1321 cgccttcggc agtcagtttt tcgtaggcag caaccgccag ttcaacttca gaaccggtgg
     1381 cgatgaagat cagttccggc tgaccagcac agtctttcag cacataacca ccgcgcgcga
     1441 tgtttgccag ctgctcttca gtacgttcct gctgcgccag gttctgacgg gagaggataa
     1501 gcgcagtcgg gccgtcctga cgctcaacgc cgtatttcca cgcgaccgcg gattcaacct
     1561 ggtcacacgg acgccatgta gacatgttcg gggtcacgcg cagagaagcg acctgctcta
     1621 ccggctggtg agtcgggcca tcttcgccca gaccgatgga gtcgtgggtg taaaccatca
     1681 cctggcgctg tttcatcagc gcagccatac gtacggcgtt acgtgcgtat tccacgaaca
     1741 tcaggaaggt ggaggtgtac ggcaggaagc caccgtgcag ggagataccg ttagcaatcg
     1801 cggtcatacc gaactcgcga acaccgtagt ggatgtagtt acctgcagca tcttcgttga
     1861 ttgctttaga accagaccac agggtcaggt tagacggtgc caggtcagca gagccgccga
     1921 ggaattcagg caacagcggg ccgaacgctt cgatagcatt ctgcgacgct ttacggctgg
     1981 cgattttcgc cggattagcc tgcagtttag cgataaactc tttcgctttg gcgtcgaagt
     2041 cagacggcat ttcgcctttc atacggcggg taaattcagc cgcttcctgc ggataagctt
     2101 tcgcgtaagc cgcaaacttc tcattccatg cagattcttt cgcctggcct gcttctttcg
     2161 catcccactg agcatagatt tcagacggga tttcgaacgg tgcatatttc cagcccagct
     2221 gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc accgtgggag tcgtgagtac
     2281 cggctttgtt cggggaaccg aaaccgatga tggttttgca catcagcagg gacggtttgt
     2341 cagtcactgc gcgcgcttct tcaactgcgc gtttgatgga tgccgcgtca tgaccgtcga
     2401 tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat tgctgtgtcg tcggtgaacc
     2461 agccttcaac gtgaccatcg atagaaatac cgttgtcatc gtagaacgca atcagtttac
     2521 ccagcttcag cgtacccgcc agagagcaaa cttcgtggga gatgccttcc atcatgcagc
     2581 cgtcgcccat gaaggcgtag gtgtagtggt cgacaatgtc gtggcccgga cggttaaact
     2641 gcgccgccag cgttttttct gcaatcgcca taccgactgc gttggcaata ccctgaccca
     2701 gcggaccggt ggtggtttcc acaccagcgg tataacctac ttccgggtgg cccggagttt
     2761 tagagtgcag ctgacggaag tttttcagtt cttccatcgg cagatcgtaa ccggtgaggt
     2821 gcagcaggct gtagatcagc atggagccgt ggccgttgga cagcacgaag cggtcacggt
     2881 cagcccagga cggattctgc gggttgtgtt tcaggaaatc acgccacagg acttcggcaa
     2941 tgtcagccat acccataggg gcacccgggt gaccggattt ggctttctgt actgcgtcca
     3001 tgctcagcgc acgaatagca ttggcaagct ctttacgtga ggacattttg actccagatc
     3061 ggatgatgaa gggcacgccc ttaacgactt gacgacagcg cgttttgggc tacgccggaa
     3121 aatttgccaa caatttaccg caagccgcgc gtcatgtaca tggaacatcc ttttgccgct
     3181 tcagaaatct ctggatcatg ctcgcatgtt gcgcaatctg ctcgcccgtc cgctgcgctt
     3241 ttccttatac tgagactgag cgtcgattca cctgcaaacg gcgcattttt agaataatcc
     3301 tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt attggtagca atgagcgtgg
     3361 caacggtact gaccggttgc cagaatatgg actccaacgg actgctctca tcaggagcgg
     3421 aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa aaccctgagc gatcaggcat
     3481 gtcaggagat ggacagcaag gcgacgattg cgccagccaa tagcgaatac gctaaacgtc
     3541 tgacaactat tgccaatgcg ctaggcaaca atatcaacgg tcagccggtg aattacaaag
     3601 tgtatatggc gaaggatgtg aacgcctttg caatggctaa cggctgtatc cgcgtctata
     3661 gcgggctgat ggatatgatg acagataacg aagtcgaagc ggtgatcggt cacgagatgg
     3721 ggcacgtggc gttaggccat gtgaagaaag ggatgcaggt ggcgcttggt acaaatgccg
     3781 tgcgagtagc tgcggcctct gcgggcggga ttgtcggcag tttatctcaa tcacaacttg
     3841 gtgatctggg cgagaaatta gtcaattcgc aattctccca gcgccaggaa gcagaagccg
     3901 atgattattc ttacgatctt ctgcaccaac gcggcatcag cccggcaggt cttgccacca
     3961 gctttgaaaa actggcgaag ctggaagaag gccgtcaaag ctcaatgttt gacggccatc
     4021 ctgcatccgc cgaacgtgcc cagcatattc gcgatcgcat cagcgcggat gggattaagt
     4081 aaagcctggt ggtgtcgaac gtaggtcaga taaggcgttc acgccgcatc tgacattaat
     4141 ggcacatttg ttaccttgtg cgcattgccg gatgcgatgc tggcgcatct tatccggcct
     4201 acgggtgccg aacgtaggtc ggataaggcg ttcacgccgc atccgacatt aatggcacgt
     4261 tttacccgtg cgcatcgcat ctggtgctta ctcgcccttt ttcgccgcct gaatatacag
     4321 catctccagc gccagcgtcg ccgcagccag agcagtgatt tccgactgat cgtatgccgg
     4381 agccacttcc actacgtcca tcccaacgat gttgagatct ttcaggccgc gtaccagttt
     4441 aatggcgcga tcggaggtca ggccgccaat cactggcgta ccggttcccg gcgcgaaagc
     4501 aggatccagg cagtcgatat caaaagtcag gtaaaccggc atatcaccca caatctgttt
     4561 cacctgggcg ataacgtcat ccacgctgcg atcgttcacc tggcaggcgt ccagcacggt
     4621 aaagccgttg tctttatcaa actcggtacg aataccaatc tgcacggaat gattcgggtc
     4681 gatcagacct tctttcggcg cggtatagaa catagtgccg tggtcaaatt cacaaccgtt
     4741 cgcataggta tcggtgtggg cgtcaaagtg taccagcgcc attttgccga aatgcttcgc
     4801 atgagcacgc agcagcggca gcgtaacaaa gtggtcacca ccgaaggaga gcatacgctt
     4861 accggcagcc agcagcttct cggcgtgcgc ctgcagcttt tcgctcatct cacgggcatc
     4921 gccaaaggca tataccagat cgccgcagtc cacgacgttc agacgctcac gcatgtcgaa
     4981 attccacggg aagcggttgt gttcccaggc cagattcgtc gaaacctgac ggatcgctgc
     5041 cggaccgtgg cgaccacccg cacgaccaga agtggccata tcgaacggca cgccagtaat
     5101 cacccagtct gcatcgctgt catacggctg gaagttcatc ggcaggcgta aaaaaccaaa
     5161 ggcattggaa accagtgagt tatcgtattg atgacctaag gtgctcatgg atggactcct
     5221 attacaaagt cgatataaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADMFL010000191         5339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.197, whole genome shotgun sequence.
ACCESSION   DADMFL010000191 DADMFL010000000
VERSION     DADMFL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.197"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="JNZ27_003921"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="JNZ27_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY8666044.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="JNZ27_003922"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="JNZ27_003922"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY8666045.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="JNZ27_003923"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="JNZ27_003923"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY8666046.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="JNZ27_003924"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="JNZ27_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY8666047.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1290 c         1456 g         1284 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac tctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gtgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaaattaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DADMFL010000192         5302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.198, whole genome shotgun sequence.
ACCESSION   DADMFL010000192 DADMFL010000000
VERSION     DADMFL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.198"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(178..594)
                     /locus_tag="JNZ27_003925"
     CDS             complement(178..594)
                     /locus_tag="JNZ27_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666048.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(653..1543)
                     /gene="rarD"
                     /locus_tag="JNZ27_003926"
     CDS             complement(653..1543)
                     /gene="rarD"
                     /locus_tag="JNZ27_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY8666049.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1595..2062)
                     /locus_tag="JNZ27_003927"
     CDS             complement(1595..2062)
                     /locus_tag="JNZ27_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY8666050.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2227..3096
                     /gene="pldA"
                     /locus_tag="JNZ27_003928"
     CDS             2227..3096
                     /gene="pldA"
                     /locus_tag="JNZ27_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY8666051.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3229..5058
                     /gene="recQ"
                     /locus_tag="JNZ27_003929"
     CDS             3229..5058
                     /gene="recQ"
                     /locus_tag="JNZ27_003929"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY8666052.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5122..>5302
                     /locus_tag="JNZ27_003930"
     CDS             5122..>5302
                     /locus_tag="JNZ27_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8666053.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
BASE COUNT         1378 a         1297 c         1333 g         1294 t
ORIGIN      
        1 ttcatttgaa tagttaatcg ttgtcttact tttaatgctt taaatgctgc tttatattct
       61 ggtgttgctc cccagaatcc aggaaagcca tgttgatcat aaaaattaaa tcggtatttc
      121 catttttcac ttaatgaatc atcgttcata tattccttac tcataaatac tccataacta
      181 ttgttttgat gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat
      241 caaaaaaatc atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat
      301 agagatgata tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact
      361 atcacggagt cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac
      421 tataacaaga aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa
      481 atttattaaa ataaatgaaa atatatatcg gcgtctggaa atcttaccgt tagatgttgg
      541 gataaacata cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat
      601 atgcccactc tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg
      661 acgttctacg ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa
      721 aggtgaaagt caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca
      781 ggaacatcag cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg
      841 tggcagcggc agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca
      901 gtaaattcag cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa
      961 acaggtaaat cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg
     1021 cttctaccgc aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca
     1081 gcgcgataat aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca
     1141 gaatcaccgc cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca
     1201 caatgttcac cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt
     1261 tcaccgccca gataaacagt agccagttgc caccaatcag cacggcagag actgccagca
     1321 taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc
     1381 tcatcagcac caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg
     1441 cgggcacgta gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg
     1501 caagagcgag taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt
     1561 aattggtaac agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg
     1621 cactggcaat ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat
     1681 tgcctgcacg caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat
     1741 aatcaacgcg aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac
     1801 tgatggtttc gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca
     1861 gcgccgacgc aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg
     1921 gctgattttt aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca
     1981 atgcgcggtt aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt
     2041 cagcggtcag tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt
     2101 ttatgcttct ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta
     2161 agtatgtaaa atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca
     2221 cgaccgatgc ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta
     2281 tatgcacaag aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt
     2341 atcgccaata tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac
     2401 tacctcatct acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg
     2461 gcggaaaatg cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg
     2521 cgtgggattt taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg
     2581 caactgtcca atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg
     2641 ttcctcggtt ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg
     2701 gggtataacc acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt
     2761 tatactcgcc tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg
     2821 gtgggtaata ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa
     2881 atcggctatc acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc
     2941 ggctacggcg gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat
     3001 actcaggttt acagcggcta tggagaatcg ctcatcgact ataacttcaa ccagacccgt
     3061 gtcggtgtag gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat
     3121 ctggaatcca gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc
     3181 aggcgctgaa aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg
     3241 gaagtgttga atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa
     3301 cagtttcgcc ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg
     3361 gttgttatgc ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta
     3421 aacggcctta ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa
     3481 ctacaggcca acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa
     3541 cttgaagtga tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa
     3601 cgcctgatgc tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc
     3661 gtcgatgaag cgcactgtat ctcccaatgg ggccacgatt tccgcccgga atatgccgcg
     3721 ctcggtcagt tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc
     3781 gacgacacca cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa
     3841 atcagcagtt ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc
     3901 gatcagttga tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac
     3961 agccgggcga aagtagaaga caccgctgcg cgcctgcaaa gcaagggtat tagcgcggcg
     4021 gcctatcatg ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc
     4081 gatgatctgc aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac
     4141 gttcgcttcg tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc
     4201 ggtcgcgccg ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat
     4261 atggcgtggc tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag
     4321 cgccacaaac tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta
     4381 ttgctgaact attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc
     4441 gatccgccga aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt
     4501 cgtgtgaatc agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac
     4561 cagcgtatcc gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa
     4621 agccatgaac attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa
     4681 aatattgccc agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc
     4741 gaatcctctt tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag
     4801 aaatcgttcg gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa
     4861 tcgattgccg atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt
     4921 gagatggctg aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg
     4981 atgcgcaagc tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat
     5041 ggcgacgacg aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg
     5101 tttcccccgc gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt
     5161 gtggcgctta tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt
     5221 tcccgtaaag aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct
     5281 gggattgcgt tgcttggcct gc
//
LOCUS       DADMFL010000193         5294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.199, whole genome shotgun sequence.
ACCESSION   DADMFL010000193 DADMFL010000000
VERSION     DADMFL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.199"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..583
                     /locus_tag="JNZ27_003931"
                     /pseudo
     CDS             1..583
                     /locus_tag="JNZ27_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            647..1573
                     /gene="glsB"
                     /locus_tag="JNZ27_003932"
     CDS             647..1573
                     /gene="glsB"
                     /locus_tag="JNZ27_003932"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY8666054.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1573..1932
                     /locus_tag="JNZ27_003933"
     CDS             1573..1932
                     /locus_tag="JNZ27_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY8666055.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2071..3489
                     /locus_tag="JNZ27_003934"
     CDS             2071..3489
                     /locus_tag="JNZ27_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8666056.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3716..5167
                     /locus_tag="JNZ27_003935"
     CDS             3716..5167
                     /locus_tag="JNZ27_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY8666057.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPGAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGILPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1223 a         1231 c         1424 g         1416 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gcactgaaaa aatgcgtact actatccgcc
      241 aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc
      301 cgttgctgca atcaccattg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag
      361 tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc
      421 ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt
      481 ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca
      541 cgaattctgt aatatccaga cagtgtggaa agaccggatc tgacgcgaca tattcagctc
      601 tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat
      661 ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa
      721 agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg ggattgctat
      781 ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca
      841 gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat
      901 ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga
      961 aatggaacag ggtattccgc gtaatccgtt cattaatgcc ggtgcgctgg tggtctgcga
     1021 tatgttgcaa gggcgactaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt
     1081 aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc
     1141 cgcgcgaaat gcggctatcg cttggctgat gaagtcgttt ggcaatttcc atcatgacgt
     1201 gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct
     1261 ggcccggacg tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt
     1321 ggtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca
     1381 gaacgcgggg gagtttgcct ggcgggtggg actaccggcg aaatctggcg ttggtggcgg
     1441 tattgtggcg attgttccgc atgaaatggc catcgcagtc tggagtccgg aactggatga
     1501 tgcaggtaac tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg
     1561 ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt
     1621 cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca
     1681 gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct
     1741 aatgacggta agcaaccccc catgcgcggg catccggtgt ttatcgctca gcacgccacc
     1801 gcgacctgct gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta
     1861 agcgaagaac aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg
     1921 aatcagccat gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc
     1981 cgccgctgtt agcgaacatg ctatccttta tgagaattta cttatcacgt tgagataatt
     2041 gccacccgca attgtatgga cagataatta atgcatgtac accccatttc aactttccgt
     2101 ttgttccagg aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg
     2161 ttctatttta ttggtgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg
     2221 aatggcgtaa tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat
     2281 gcgatcagtt atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt
     2341 tcactggtta tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg
     2401 cgggataagc gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat
     2461 tactgtctga ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt
     2521 gatagtctgg atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc
     2581 gtgttgatcg tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa
     2641 gcagaacaga tgatgcccgc catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt
     2701 ggcggagcgg ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat
     2761 acgccgcagg taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtgctggtg
     2821 gcaaattcgg tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc
     2881 gcacgtctcg gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca
     2941 gcgatcaatt tgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag
     3001 gtttactcac ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa
     3061 catctgacgg tcatgctgct tgatatcgac tatttcaaaa gcattaacga taactatgga
     3121 catgaatgtg gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt
     3181 gataaggggc tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg
     3241 aatcctgtcg atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca
     3301 ttcacctggc aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc
     3361 gcatcgtacc gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt
     3421 ctgtatcgct cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa
     3481 gttgtctgac atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat
     3541 gatccaggaa aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata
     3601 aaactgctca ttaatcatca ggagttgatc aaaacagggg ttcggcgtat taaagtttgc
     3661 taacctgtcg caccaattca aacaggttgt atgactaatc agaagggaac ccattgtgaa
     3721 aacactaaat cgtcgcgatt ttcccggtgc acagtatcca gaacgtatca ttcagtttgg
     3781 tgaaggtaac ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac
     3841 cgatctgaat tctggcgtgg tcgttgttcg tccgattgaa acttcattcc cgccgtcact
     3901 gagcacgcag gatggtctgt acaccaccat tatccgtggc ctgaatgaga agggggaagc
     3961 ggtcagcgac gcgcgtctga ttcgctcggt taatcgtgaa atcagcgtct acagtgaata
     4021 cgatgaattc ctgaaactgg cgcacaaccc ggaaatgcgc tttgttttct ctaacaccac
     4081 cgaagcgggt atcagctacc atgcgggcga taaattcgat gacgcgccag cggtaagcta
     4141 tccggcaaaa ctgacccgtc tgctgttcga acgctttagc catttcaacg gtgcgctgga
     4201 taaaggttgg atcatcattc cgtgtgagtt gattgactac aatggcgacg cgttgcgtga
     4261 actggtgctg cgctatgcgc aagagtgggc actgccagga gcatttattc aatggctgga
     4321 tcaggctaac agcttctgtt ctacgctggt ggaccgtatc gttaccggtt atccgcgcga
     4381 tgaagtggct aaactggaag aagaactggg ttatcacgat ggtttcctcg acaccgctga
     4441 acacttttac ctgtttgtga ttcaggggcc gaaatcctta gcgactgagc tgcgcctgga
     4501 caaatatccg ctcaacgtgc tgattgttga cgatattaaa ccgtataaag aacgcaaagt
     4561 ggcaatcctc aacggtgcgc acaccgcgct ggtgccggtg gcgttccagg cagggctgga
     4621 taccgtaggt gaagcgatga acgatgctga aatttgcgca ttcgttgaaa aagccatcta
     4681 cgaagaaatt attccggtac tggatttgcc tcgtgatgaa ctggaatctt tcgccagtgc
     4741 agtcaccggg cgtttccgta acccgtacat taagcatcag ctgctgtcta tcgcgctcaa
     4801 cggtatgacc aagttccgca cccgcatcct gccgcagctg ctggcagggc agaaggcaaa
     4861 cggcatactt ccggcgcgcc ttactttcgc attagcggca ttgattgcgt tctatcgtgg
     4921 tgagcgtaac ggagaaacat atccggtaca ggacgatgca cactggctgg aacgttatca
     4981 gcaactctgg agccagcatc gtgaccgtgt tatcggtacc caggaactgg tagcgattgt
     5041 gttggcagaa aaagaccact gggagcagga cctgacgcaa gtgcctggtc tggtcgagca
     5101 ggttgctaat gacctggatg cgattcttga aaaagggatg cgcgaagctg tacgtccgtt
     5161 gtgctaataa caccgttact tctgaacccg gtttcgaccg ggtttcttat ttcactatat
     5221 tagttacaac attctatatg actttatcat taacaggtgt cacatgcagc gcctgttaat
     5281 gttgtaagct tgac
//
LOCUS       DADMFL010000194         5180 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.200, whole genome shotgun sequence.
ACCESSION   DADMFL010000194 DADMFL010000000
VERSION     DADMFL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.200"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(301..390)
                     /locus_tag="JNZ27_003936"
     CDS             complement(301..390)
                     /locus_tag="JNZ27_003936"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666058.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(826..987)
                     /locus_tag="JNZ27_003937"
     CDS             complement(826..987)
                     /locus_tag="JNZ27_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8666059.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(1639..1830)
                     /locus_tag="JNZ27_003938"
     CDS             complement(1639..1830)
                     /locus_tag="JNZ27_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8666060.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1895..2218
                     /locus_tag="JNZ27_003939"
     CDS             1895..2218
                     /locus_tag="JNZ27_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY8666061.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQRSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            2208..2501
                     /locus_tag="JNZ27_003940"
     CDS             2208..2501
                     /locus_tag="JNZ27_003940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666062.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVLMPGLEKNQYSILWVEH"
     gene            2541..2918
                     /locus_tag="JNZ27_003941"
                     /pseudo
     CDS             2541..2918
                     /locus_tag="JNZ27_003941"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2896..3426
                     /locus_tag="JNZ27_003942"
     CDS             2896..3426
                     /locus_tag="JNZ27_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY8666063.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            3433..3666
                     /gene="mbeD"
                     /locus_tag="JNZ27_003943"
     CDS             3433..3666
                     /gene="mbeD"
                     /locus_tag="JNZ27_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY8666064.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(4236..>5180)
                     /locus_tag="JNZ27_003944"
     CDS             complement(4236..>5180)
                     /locus_tag="JNZ27_003944"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8666065.1"
                     /translation="ISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLLILL
                     SYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIGCVI
                     MLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLPEFM
                     VGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCISIII
                     YKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFILLMV
                     FFLTFYLSKKILIPTSKIESILIKKMNIINAKKANKTS"
BASE COUNT         1430 a         1088 c         1342 g         1320 t
ORIGIN      
        1 aaaaaaaaac aagctcacac ctacctggcc aagaacccct ccataaaatg atggtatgaa
       61 agatagcctg gtttggctaa atatgtagcc ataatgagat ataataacaa aaatgcaggc
      121 tgttactcgc aatgtatcaa ttgacggatc tctattagac ataaccttta tttcgctgtt
      181 caaattatcg cctttagttg atgatgtaaa attaacgcta taaataagct acacattcta
      241 gagcagcgaa aggatgattc aagtattttt aataataaaa cgcattgata atgtttatgt
      301 tcagatggtg ctgatagctt tgtcatgctg ttccactgat gttgataacg acagagactt
      361 ttgtcctttt cttgttagat gctgcctcag attcggatta tggcgttaaa ggggtttgac
      421 gctcaaccga acggtttcac acgttaagag ctttttgtct gataaatcaa aatataatgg
      481 acattgaaaa acgccctcaa aacggtgtct ttttaatcga agattggcac aaaacggaca
      541 ccaccgtcca ttagtcgatt ttttagacgg cttttctcgc ctaacgtgag ttttcgttcg
      601 gttgagcatc agaccccttt tatattgtag atttctattt ttagacagtt ggaacaggac
      661 aattaacaac agatctactt gagatcattt gcttgtcgtc gtagtctctt tcccagaaac
      721 gaaaaaaccg ccctgcaagg cggtttctcg aaggttctca gagcgaccaa ctctttgaac
      781 caaggtaact ggcttatgag gagcgcagta accaaatctg tcctttcagt ttagccttaa
      841 caggcgcata acttcaagac taactcctct aaatccgtta ccaatggcta ccgccactgg
      901 cgatttgtcg tgtctttccg ggttggactc aagttgatag ttaccggaac gggcgtggta
      961 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     1021 actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa
     1081 accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc
     1141 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcggtttttg tgatgctcgt
     1201 caggggggcg gagcctatgg aaaaacgcca tcggcgcggc cttcttcctg ttcctggtct
     1261 tttgctcaca tgttctttcc ggcgttatcc cctggttctg tggataacag catttcgttt
     1321 ttgagtgagt agataccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     1381 aggaagcgga aaagcgcctg gacgtgcatt ttctccttac gcatctgtgc cgcatttcac
     1441 acccggtatg gcgcactttt catacaatct gcactgatgc cgcatggtta agcccgtata
     1501 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc taaaacctgc
     1561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacagacaag ctgtgaccgt
     1621 ctccgggagc tgcatatgtc agaggttttc accgtcatcc ccgaaacgtg cgaggcagct
     1681 gcggtaaagc tcatcggcgt ggtcgtggag cgattcacag atatcagcct gttcatccgc
     1741 gtccagttcg ttgagcttct ccagcagcgt taatgtctgg cttctgataa agcgggccat
     1801 gttaagggcg gttttttcct gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt
     1861 ttatggggta atgataccga tgaatcgcga gaggatgctc acgatacggg ttactgatga
     1921 tgaacatgcc cggttactgg aacgctgtga gggtaaacag ctggcggtat ggatgcggcg
     1981 ggtctgcctg ggtgagccgg ttgcccgttc cgggaaactg ccgacactgg caccgccgtt
     2041 actgcgtcag ctggctgcca tcgggaataa cctgaatcag acagcgagga aggtgaacag
     2101 cgggcagcgg tcttccggta accgggttca ggtggtggcc gcactgatgg ccatcgggga
     2161 cgagctgcgc cggctgcgtc tggccgtcag ggaacagggg acgcgggatg atagttaagt
     2221 ttcatgcccg gggaaaaggt ggtggcagtg gtccggttga ttacctgctg ggaagggagc
     2281 gtaaccggga aggggcaacg gtgcttcagg gtaatccgga agaagtccgg gaactcatcg
     2341 atgccacgcc atttgcgaag aaatacacgt ccggtgttct gtcgttcgcg gagaaggagc
     2401 tgacgccggg aggacgtgaa aaagtgatgg cgagctttga gcgtgtactg atgcccggtc
     2461 tcgaaaaaaa tcagtacagc atcctgtggg tggagcacta ggacaaggga cggcttgagc
     2521 tgaattttgt cattccgaac atggagctac agaccggaaa acgcctccag ccgtactacg
     2581 accgcgcaga caggcctaga attgatgcct ggcagacgct ggttaatcac cattacgggc
     2641 tgcatgaccc gaacgccccg gagaaccgca ggacgctgac actccctgat aatctgccgg
     2701 aaacgaaaca ggcgcttgca gagagcgtca cacgcgggat agacgctctt tatcatgtcg
     2761 gggagataaa aggccgtcag ggtgtgattc aggcgctcac agaggcaggg attgacgtgg
     2821 tcagggtgac gcgaagcagt atcagcattg cagacccgaa cggtgggaag aatatcaggc
     2881 tgaaaggagc attctatgag caatctttta cagacgggcg cggagttcga gaaaaagctg
     2941 aaagagagag ccgaatctac agagaaaatg ctgaacgacg agttcaggaa gctcgaagaa
     3001 tctgtaaaca gggaactgac ctcaaacgaa tctttaatca ggaacgctat caacgaccat
     3061 acatcagccc tgaaagaact tctggagagg tatcagaaaa cgaccgtcga tacgatggac
     3121 gcacactgga aaaccgtact gaaaatgagc gtgaaacgct ggctgtggct gattatcgtc
     3181 tcagtactga tgtttgccac gaccgggagc ctgctgtggt gccagggcat gaagataaac
     3241 gcgaacatga atattctccg cgagcagaaa gagagtctgg agaagctgaa cgcgaagaca
     3301 tggggcgtgc gttatcacga ggacagcaac ggacgttttc tggtgttgcc cgaggggatg
     3361 aaagccgaaa cgaactggac gatggaaaac gggaaacaga atgccgtaag gctggtacag
     3421 gagtaacaga gcatgacgga gctggaaaga catttgctga acgcattaga gcaactgcaa
     3481 caggactata tgcagcggct gaacgaatgg gagagcgcct tcgtggaatt gcagaaaatg
     3541 ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag
     3601 caggtgcagc acttgagcga gcagacagaa cgcttgagcc agttatacag cgagaacagg
     3661 agataaggga cgaacggctg atacaggaat gcgaacatgc gttatccctg gagcgtgagc
     3721 gacagcagga aatacaggaa cggacactgg atggcccttc gctgggatgg taatcagaga
     3781 caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt gggataatgg ttggtttggg
     3841 ggcaccggga acgcgcttca gtcagacctt gctgaaaaca taggaacgcg ttaaaacgca
     3901 tacgggcgcg attctggtgt gtcttttgtt gctgtgtttt gcctctattg ccagatcgcg
     3961 ctcattcccc tgaacttctg ttttcccgct caggtccccg tcttcttcgc ttccgaaagc
     4021 atgaaaaaaa tccgtttcag ctgtctctgg ctggcaattc ctgcgtgatt cacatggctg
     4081 catagctatg catgtggtta aaatttaccg ggtgcgatcg cggcagtttt tcgggtggtt
     4141 tgttgccatt tttacctgtc tgctgccgtg atcgcgccga acgcgtttca gcggtgcgta
     4201 caatgagatg ttatggtaaa ttcaagtctt agattttatg atgttttatt agcttttttt
     4261 gcgtttataa tgttcatctt ttttattaaa attgattcaa tctttgatgt gggtattaat
     4321 attttctttg acaaatagaa agttaaaaaa aacaccatta atagtataaa taaataccca
     4381 aaaacagaag cgttattaat ttcaattatt gatgttaatc tgtatattac tacatgctga
     4441 atcaacatgg atatgtagat gcatttagca tatgagttga aaaaatagag gttaaatttc
     4501 ttattaatta ttaatgctgc tttgtaaatg ataatgctta tgcacattaa aggtattatt
     4561 gataactggt gattgactat gtttgttttg aactcattag gccctattaa taatactgtc
     4621 aatatacata taacaaccga tagaataact gtggtgtttt tttgtaattt atttttgtat
     4681 ttaaatgcaa gcatcccaac cataaactca ggaagtcggc aagcaaaaaa ccaaaatggg
     4741 aagtttaaaa atgatgatga gatatttgat atcgggaatg ttgtaattgt tatcacaaat
     4801 aaaaaaaata tgttacgttt aaatgtaaag gtattcataa cgtgaaaaac tatcggtgat
     4861 atcacgtaca gcataataac gcacccaata aaccactcgc ctgtaaggaa aagaaaatca
     4921 taagagaaat gggcgtttaa tataccatca agggcaaaaa atactggaat tattctagtg
     4981 tactggcctc ctgttgtcat tattgatacg agagggactg tattaattat ctctttattt
     5041 aaataagaca ataaaattaa gagtaaagag ataaaaaagt aagtgataat ataaggagta
     5101 aggatcctta ctcctttgtc aaggaaataa tcaatggtgt tctttctatc tagtgaaata
     5161 aatgccaaat atccagatat
//
LOCUS       DADMFL010000195         5145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.201, whole genome shotgun sequence.
ACCESSION   DADMFL010000195 DADMFL010000000
VERSION     DADMFL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.201"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            190..447
                     /locus_tag="JNZ27_003945"
                     /pseudo
     CDS             190..447
                     /locus_tag="JNZ27_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNZ27_003946"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNZ27_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY8666066.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JNZ27_003947"
     CDS             complement(1599..2351)
                     /locus_tag="JNZ27_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY8666067.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNZ27_003948"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNZ27_003948"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY8666068.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JNZ27_003949"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JNZ27_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..4420)
                     /locus_tag="JNZ27_003950"
     CDS             complement(3605..4420)
                     /locus_tag="JNZ27_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8666069.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5145
                     /locus_tag="JNZ27_003951"
     CDS             4517..>5145
                     /locus_tag="JNZ27_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8666070.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLE"
BASE COUNT         1373 a         1170 c         1225 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgctaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagtgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctagcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaata
//
LOCUS       DADMFL010000196         5143 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.202, whole genome shotgun sequence.
ACCESSION   DADMFL010000196 DADMFL010000000
VERSION     DADMFL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.202"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..253
                     /locus_tag="JNZ27_003952"
                     /pseudo
     CDS             1..253
                     /locus_tag="JNZ27_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="JNZ27_003953"
     CDS             746..1492
                     /locus_tag="JNZ27_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8666071.1"
                     /translation="MKNIAIGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQEVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="JNZ27_003954"
     CDS             1507..1929
                     /locus_tag="JNZ27_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY8666072.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="JNZ27_003955"
     CDS             1987..2343
                     /locus_tag="JNZ27_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666073.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="JNZ27_003956"
     CDS             2436..2654
                     /locus_tag="JNZ27_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY8666074.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="JNZ27_003957"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="JNZ27_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3683
                     /locus_tag="JNZ27_003958"
     CDS             3018..3683
                     /locus_tag="JNZ27_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666075.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3683..4048
                     /locus_tag="JNZ27_003959"
     CDS             3683..4048
                     /locus_tag="JNZ27_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8666076.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4446..4658
                     /locus_tag="JNZ27_003960"
     CDS             4446..4658
                     /locus_tag="JNZ27_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8666077.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            4895..>5143
                     /locus_tag="JNZ27_003961"
     CDS             4895..>5143
                     /locus_tag="JNZ27_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666078.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1408 a         1088 c         1420 g         1227 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaatggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg ataggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggaggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg cggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgacaaa ctgctggatg aattgcgagt cagagcatcc tgt
//
LOCUS       DADMFL010000197         5129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.203, whole genome shotgun sequence.
ACCESSION   DADMFL010000197 DADMFL010000000
VERSION     DADMFL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.203"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..238
                     /locus_tag="JNZ27_003962"
     CDS             <1..238
                     /locus_tag="JNZ27_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666079.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            278..818
                     /locus_tag="JNZ27_003963"
                     /pseudo
     CDS             278..818
                     /locus_tag="JNZ27_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            885..2303
                     /locus_tag="JNZ27_003964"
     CDS             885..2303
                     /locus_tag="JNZ27_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY8666080.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2284..2754
                     /locus_tag="JNZ27_003965"
     CDS             2284..2754
                     /locus_tag="JNZ27_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY8666081.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2743..3663)
                     /gene="yedA"
                     /locus_tag="JNZ27_003966"
     CDS             complement(2743..3663)
                     /gene="yedA"
                     /locus_tag="JNZ27_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY8666082.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3836..4753
                     /locus_tag="JNZ27_003967"
     CDS             3836..4753
                     /locus_tag="JNZ27_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY8666083.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4832..5014
                     /locus_tag="JNZ27_003968"
     CDS             4832..5014
                     /locus_tag="JNZ27_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY8666084.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1227 c         1465 g         1185 t
ORIGIN      
        1 gtgggctttc tatacatggt gtggctattt cgtctgggca atggcgcgat acatctgggt
       61 gatgagccgg atccccgatg cccccgtgtc gggatttgaa agtgatttag gatctaccgc
      121 ggggaagtgg ttaggagcat tggtcggatt tctgtttatg gccctggttg gtgccttgtt
      181 aggcggcatt gcatggtaca cccgacctcg tcccgcacgt tgcagacgct atgaataaaa
      241 actgctaagg ttgcgactct ttggcaacgg agcgcgtatg gagttacaac actggcaggc
      301 acagttcgag aactggttga aaaaccatca tcaacatcag gacgcggcgc atgacctgtg
      361 tcattttcgc cgtgtctggg ctacggcaca gaaactggcg gcagatgacg atgtcgatat
      421 gctggtgatt ttaaccgcct gttattttca cgatatcgtg agcctggcga aaaatcatcc
      481 gcaacggcag cgttcttcaa tcctggcggc agaagagacg cgtcgcctgc tccgtgaaga
      541 gttcgtgcaa tttccggcgg agaaaattga ggccgtttgt catgccattg ccgctcacag
      601 tttcagcgcg caaatcgccc ccttaacaac ggaagctaaa atcgtccagg atgcggatcg
      661 gctggaagcc ctgggggcga ttggcctggc gcgcgtattt gccgtttcag gggcattgag
      721 cttggcgctg tttgatggcg aagctccgtg ccgaactggc gggggagaat gaaggtatcg
      781 atcacaaggt gatagatgcg ttttcacccg ctggctgagc gcgtggccct aaacggcagt
      841 aattatgtta acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat
      901 cagtaaccga ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc
      961 aaatttatga tgttaaaatg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga
     1021 gcgcggcaat tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga
     1081 aagagtttgc ccatctgcaa acgttgttac ccaaaccacc ggaacatcat ccgcattatg
     1141 cgtttcgctt tatcgatcta tttgccggaa ttggcggcat ccgtcgtggt tttgaatcga
     1201 ttggtggaca gtgcgtgttt accagcgaat ggaacaaaca tgcggtacgc acttataaag
     1261 ccaaccatta ttgcgacccg gcgacgcatc attttaatga agatatccgc gacatcaccc
     1321 tcagtcatca agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc
     1381 ctgaacacga tgttttactg gccggtttcc cttgtcagcc attttcgctg gctggcgtat
     1441 cgaaaaagaa ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt
     1501 tctttgatgt ggtgcgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg
     1561 tcaaaaacct gaagagtcac gataagggaa aaacgttccg catcatcatg cagacgctgg
     1621 acgaactggg ctatgacgtt gccgatgcag aagataacgg gccggacgat ccgaaaatca
     1681 tcgatggtaa acatttcctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc
     1741 gcgatctgaa tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac
     1801 agcgagtgac gctggcgcag ctgctggacc cgatggttga ggcgaaatat atcctgacgc
     1861 cggtgctgtg gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct
     1921 tcggttatgg aatggtttat ccgaacaatc cgcaaagcgt cacccgtacg ctgtctgctc
     1981 gttattacaa agatggcgcg gaaattttaa tcgatcgcgg ttgggatatg gccacgggtg
     2041 agaaagactt tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcgtg
     2101 aatgcgcgcg cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt
     2161 cggacactca ggcctatcgc cagttcggca actcggtggt cgtgccggtc tttgccgcgg
     2221 tggcaaaact gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac
     2281 aacatggtcg acgttcacga taaggccacc cgcagcaaaa atatgcgcgc gattgccacg
     2341 cgtgatacgg cgattgagaa gcgcctcgcc agtttgttaa ccgggcaggg ccttgcgttt
     2401 cgcgttcagg acgccagtct gcccggacgc ccggattttg tcgttgatga atatcgctgc
     2461 gtgatattta cccacggctg cttctggcat catcatcact gctatctgtt taaagtgcct
     2521 gcgactcgaa ctgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc
     2581 gatatcagtc gcttgcagga gctcggctgg cgcgtactga ttgtctggga gtgtgcgtta
     2641 cgtaggcgcg agaagctgac ggatgcggcg cttaccgagc gtctggaaga gtggatctgc
     2701 ggcgaaggtg ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcatc
     2761 ctgaataact ggcgcgacaa cgggtttggc cgggaagaga tattttccca acgtgaccag
     2821 taccaccgcg aaaacaatta cgccgagcgc cagccattca atcttcgaca gcgtttcgcc
     2881 acccagtccc gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc
     2941 gagagccgga ctgacattac ggattaaata catataagcg ttaatggcga taatcgaacc
     3001 aaacagcgcc agatagccga ccgcaaggaa gcctgacagg gaagggagcg ccgtcagttt
     3061 ttcacccgca atcatcgatg cgatcattaa caccacgcct gccgccagca tctcaatcgc
     3121 acccgccatc atccctacag gtaaggtaat gcgcgagcca taaactgagc caaacgccca
     3181 gctaatcgag ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc
     3241 gctattgagc atgatgattc cggcaagccc aatggcaata cccacccatt ccagtttgcg
     3301 cgttttaatg ccaaacaggc gactgaagca cagggtaaag aggggcacgg ttgcaatcac
     3361 tacggcggcg atgccggaag gaacattttg atgttcggca accgtcacca tgccattacc
     3421 gacagccagc aataacaggc caatcagcgc ggcattgagc agcggacgta gcgggggaag
     3481 tttgtgtccg cgcagtagca aaaatgccag caataaaata ccggctgcca ggaatcgaac
     3541 gcccgccatc attaacggag gccagcttcc cacgccaatc cgaatgacaa aataggttga
     3601 gccccaaatg atatacaacg caaacagagc gccaaaaagc ggtaacaact ggcggaaacg
     3661 cataatccct cacggcagaa ataaaaaggt ggttcatagt aaacgtgaaa atcattctgc
     3721 tggcgagaga tataattgca cttgattgtt aaataaatgt tgacctgtga agcaagtatc
     3781 agagctgccg tttttgcttc atacttacac ccttcagcaa taaaaatgag agggaatgct
     3841 tttggccggg agtagtttac tgacgttgct cgatgatatc gccacactgc tggacgatat
     3901 ctctgtgatg ggcaaactgg cggcgaagaa aaccgccggt gtattagggg atgatttatc
     3961 gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg gaacttcccg tggtctgggg
     4021 cgtggcgaaa ggatcgctga tcaataaagt gattctggtg ccgctggcgc tgatcatcag
     4081 tgcgtttatc ccgtgggcga ttacgccact gttgatgatt ggtggcgcgt ttctctgctt
     4141 tgaaggggta gagaaagtgc tgcatatgct ggaggcgcgt aaacataaag aagatccagc
     4201 gcagagccag cagcgtctgg agaagctggc ggcgcaggat ccgctgaagt ttgaaaagga
     4261 caaaattaaa ggggcgattc gtaccgattt tattttgtct gcggaaatcg tcgccatcac
     4321 gctggggatt gtggcagagg cgccgttgct taatcaggtg ctggtgcttt caggcatcgc
     4381 gctggtagtg accgtgggcg tctacggtct ggtaggggtt atcgttaaga ttgatgacct
     4441 agggtattgg ctggcggaaa aatccagcgc gctgatgcag gcattaggta aagggttatt
     4501 gattatcgcg ccttggctga tgaaagcatt atcgattgtc ggcacgctgg cgatgttcct
     4561 cgtcggcggc gggattgtgg tacatggtat tgcgccgctg catcacgcta ttgaacatat
     4621 cgccgggcag caaagtgcag tggtggcgat gatattaccg actgttttaa atctgattct
     4681 tggatttatc atcggcggca tcgtggtgct gggagtgaaa gcagtagcga aaatgcgcgg
     4741 tcaggcacat taatactcgc actggctatt atgcaaaatt cgccatcttc aactaaggtg
     4801 aacaagtttc actcgaaaaa aggaggcgag tatgagcttt atggttagtg aggaagttac
     4861 ggttaaagag ggcggcccgc gaatgattgt cactggatac tccagcggta tggttgagtg
     4921 tcgttggtat gacggttacg gggtcaagcg ggaagctttt catgaaaccg atcttgttcc
     4981 gggggagggg agtcgttctg cggaagaagt ttgaaagggt atcattaaca ccaggccgga
     5041 tcacatccgg cctggtgtcc caatgcctgc aatgaatgat agtcgcgtct tatcatgcca
     5101 ccattataaa attatcgctg ttttgcggt
//
LOCUS       DADMFL010000198         5039 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.204, whole genome shotgun sequence.
ACCESSION   DADMFL010000198 DADMFL010000000
VERSION     DADMFL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.204"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            108..1223
                     /gene="adrA"
                     /locus_tag="JNZ27_003969"
                     /gene_synonym="dgcC"
     CDS             108..1223
                     /gene="adrA"
                     /locus_tag="JNZ27_003969"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY8666085.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1240..2049)
                     /gene="proC"
                     /locus_tag="JNZ27_003970"
     CDS             complement(1240..2049)
                     /gene="proC"
                     /locus_tag="JNZ27_003970"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY8666086.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2169..2657
                     /locus_tag="JNZ27_003971"
     CDS             2169..2657
                     /locus_tag="JNZ27_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY8666087.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEKWWLEKWWLEVQRS
                     RG"
     gene            2840..3364
                     /gene="aroL"
                     /locus_tag="JNZ27_003972"
     CDS             2840..3364
                     /gene="aroL"
                     /locus_tag="JNZ27_003972"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY8666088.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3414..3605
                     /gene="yaiA"
                     /locus_tag="JNZ27_003973"
     CDS             3414..3605
                     /gene="yaiA"
                     /locus_tag="JNZ27_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY8666089.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3863..4540
                     /locus_tag="JNZ27_003974"
     CDS             3863..4540
                     /locus_tag="JNZ27_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY8666090.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4612..4896
                     /gene="ppnP"
                     /locus_tag="JNZ27_003975"
     CDS             4612..4896
                     /gene="ppnP"
                     /locus_tag="JNZ27_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY8666091.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1222 a         1190 c         1338 g         1289 t
ORIGIN      
        1 ggggcggttc agatagtgct aacgggtgag ctacgaaagt ggctcaccct aaatttcata
       61 cttctgcctt tagccccgtc tctataattt gggaaaattg tttctgaatg ttcccaaaaa
      121 taatgaatga tgaaaacttt ttcaaaaaag cggcggcgca tggggaggaa cctcctttaa
      181 ctcctcaaaa cgaacatcag cggtccgggc tgcgcttcgc ccgtcgcgtc agactacccc
      241 gtgcggttgg cctggctggc atgttcttac cgattgcttc aacgctggtt tcgcacccgc
      301 cgccgggctg gtggtggctg gtgttggtcg gctgggcgtt cgtctggccg catttagcct
      361 ggcagatagc gagcagggcc gtcgatccgc ttagccggga aatttacaac ttaaaaaccg
      421 atgcagtatt agcgggaatg tgggtaggcg taatgggcgt aaacgtgctg ccttccaccg
      481 ctatgttgat gattctgtgt ctgaatttga tgggggcagg cggcccccgt ctgtttgtcg
      541 cgggtctggt gttgatggtg gtttcctgcc ttgtcaccct cgagcttacg ggcattaccg
      601 tgtcgttcaa tagtgcgccg ctggaatggt ggctctccct tcccattatt gtcgtttatc
      661 ctctgctgtt tggctgggtc agctaccaga cggcaaccaa actggcggaa cataaacgca
      721 ggttgcaggt catgagtact cgcgacggca tgacgggcgt gtataaccga cgtcattggg
      781 aaactatgtt acgcaatgaa tttgataact gtcggcggca taatcgcgat gcaacgttac
      841 tgattatcga tatcgaccat ttcaagagca tcaacgatac ctggggtcat gatgtgggcg
      901 atgaagcgat tgtggcgctt acccgacagt tacaaattac cctgcgcggt agcgatgtga
      961 ttggtcggtt tggcggcgat gagtttgcag taatcatgtc cggtacgcca gctgagagcg
     1021 ccattaccgc catgttacgg gtgcgtgaag ggctaaacac gttacgcttg ccgaatacgc
     1081 cacaggtaac tttacggatt agtgtggggg ttgcgccgct gaacccacaa atgagtcact
     1141 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
     1201 accgcaccga agtggcggcc tgacgtccgg cgaaagtcat caggatttgc tgagtttttc
     1261 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
     1321 cagtacgcgt accgcttcaa tggtggtacc tcccggtgag cagaccatat ctttcagtgc
     1381 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
     1441 aaatttatac gcctggacgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
     1501 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgaccaccg ggtggatcat
     1561 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
     1621 atcttctggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
     1681 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gtgcaagctg
     1741 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
     1801 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcagcaaaaa tgatgtcggc
     1861 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
     1921 ggcgacttta tccggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
     1981 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
     2041 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
     2101 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
     2161 atgactttat gacaatttgg gtggatgccg acgcgtgtcc caatgtaatt aaagagattt
     2221 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
     2281 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
     2341 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
     2401 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
     2461 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
     2521 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
     2581 cggagctgga gaagtggtgg ctggagaagt ggtggctgga gaagtggtgg ctggaagtgc
     2641 aacgtagtcg tggctaaatg taatttatta tttacacttc attcttgaat atttattggt
     2701 atagtaaggg atgtattgag attttcactt taagtggaat tttttcttta caatcgaaat
     2761 tgtactagtt tgatggtatg atcgctattc tcatgacacc ggctttcgcc gcattgcgac
     2821 ctattgggga aaacccacga tgacacaacc tctttttctg atcgggcctc ggggctgtgg
     2881 taaaacaacg gtcggaatgg cccttgccga ttcgcttaac cgtcggtttg tcgataccga
     2941 tcagtggttg caatcacagc tcaatatgac ggtcgcggag atcgtcgaaa gggaagagtg
     3001 ggcgggattt cgcgccagag aaacggcggc gctggaagcg gtaactgcgc catccaccgt
     3061 tatcgctaca ggcggcggca ttattctgac ggaatttaat cgtcacttca tgcaaaataa
     3121 cgggatcgtg gtttatttgt gtgcgccagt atcagtcctg gttaaccgac tgcaagctgc
     3181 accggaagaa gatttacggc caaccttaac gggaaaaccg ctgagcgaag aagttcagga
     3241 agtgctggaa gaacgcgatg cgctatatcg cgaagttgcg catattatca tcgacgcaac
     3301 aaacgaaccc aaccaggtga tttctgaaat tcgtagcgcc ctggcacaga cgatcaattg
     3361 ttgattttag agcgcctata cttaacgttc atcccgtgaa ataaggaaga acgatgccaa
     3421 cgaaaccgcc ttatcctcgt gaagcatata tagtgacgat tgaaaaagga aagccaggac
     3481 agacggtaac ctggtaccaa ctcagagccg atcatcctaa accagactcg ttgatcagtg
     3541 aacatccgac ggctcaggaa gcgatggatg cgaaaaaacg ctatgaggac cctgacaaag
     3601 agtgaccgca tcagactgct cggaagggat tctgagtgcc actacaaggg atctgcgtca
     3661 catttttcat aattcatgtt tttctaataa ttagaatatt aaacaataac attccattac
     3721 tggaatcatt tggaatcttt acattatgcc gtgcacgtct gctgctacgc tttttgtcat
     3781 ttgtagcaca agtaagtgtc agcagtggtg cttcacactt gcccggtaat taacgacgaa
     3841 agaaaagtaa ggtggatgaa caatgagtgc gtcgttggcg atcctgacca tcggcattgt
     3901 acctatgcag gaggttttgc cgctcctgac ggaatacatt gacgaagata atatttccca
     3961 tcatagcctg ctggggaagt taagtcgtga agaagtgatg gcggagtacg cgccagaagc
     4021 aggcgaagac accattctca cattattaaa tgacaaccag ctggcccatg tttcgcgtcg
     4081 taaagtggag cgagatctgc aaggtgtggt tgaagtgctc gataatcagg gttatgacgt
     4141 cattttatta atgagtacag caaacattag tagtatgact gcgcgtaata cgatctttct
     4201 tgagccgtcg cgaatattgc ctccactggt ttcctctatt gttgaagatc atcaggtggg
     4261 ggttatcgtt ccggttgagg agatgctgcc cgttcaggcg caaaaatggc aaattttgca
     4321 gaaatcgccg gtattttcat tgggtaaccc cattcatgat tcagaacaaa aaatcattga
     4381 tgctgggaaa gaattactgg caaaaggggc tgatgtcatc atgctggatt gtttaggatt
     4441 tcaccaacgt catcgcgatt tactgcaaaa acagctcgat gttcctgtct tgctctctaa
     4501 cgtattgatt gcacggctgg ctgcggaatt actggtgtaa ttttgcgtga cagccagcgc
     4561 ctctggcccc tatagtgaag tagatgttca actaccagac agggccagtt tatgcttcaa
     4621 agtaatgagt acttttccgg caaagtgaaa tcgatcggct tttccagcag cagcactggt
     4681 cgcgccagcg tgggtgttat ggttgaaggc gaatacacct tcagcaccgc tgagccggaa
     4741 gagatgacgg taatcagtgg cgcgctgaat gtgttactgc ctgacgcgac cgactggcaa
     4801 gtgtatgaag ccggttcggt gtttaatgtt cccggtcaca gtgagtttca tcttcaagtt
     4861 gccgaaccta cctcttatct gtgccgctat ctgtaattcc tcgccttccc cttgaacggg
     4921 agggcatttt tctgaaatat cctctcttta acccataata atatttcctt tgctgcgatt
     4981 ttttcaattt ccgatatatt cataattcat caaggttgat ataaatatca gtgaagatc
//
LOCUS       DADMFL010000199         4819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.205, whole genome shotgun sequence.
ACCESSION   DADMFL010000199 DADMFL010000000
VERSION     DADMFL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.205"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..431
                     /gene="fliR"
                     /locus_tag="JNZ27_003976"
     CDS             <1..431
                     /gene="fliR"
                     /locus_tag="JNZ27_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="FliR, with proteins FliP and FliQ, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY8666092.1"
                     /translation="LNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIGSEP
                     LNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLT
                     LTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            716..1339
                     /gene="rcsA"
                     /locus_tag="JNZ27_003977"
     CDS             716..1339
                     /gene="rcsA"
                     /locus_tag="JNZ27_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY8666093.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     TSSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(1383..1625)
                     /gene="dsrB"
                     /locus_tag="JNZ27_003978"
     CDS             complement(1383..1625)
                     /gene="dsrB"
                     /locus_tag="JNZ27_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY8666094.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1734..1961
                     /gene="yodD"
                     /locus_tag="JNZ27_003979"
     CDS             1734..1961
                     /gene="yodD"
                     /locus_tag="JNZ27_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY8666095.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            2259..3074
                     /locus_tag="JNZ27_003980"
                     /pseudo
     CDS             2259..3074
                     /locus_tag="JNZ27_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(3071..4765)
                     /gene="yedQ"
                     /locus_tag="JNZ27_003981"
     CDS             complement(3071..4765)
                     /gene="yedQ"
                     /locus_tag="JNZ27_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY8666096.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         1261 a         1157 c         1149 g         1252 t
ORIGIN      
        1 atctcaatat gcccgtttta gcgcgcatca tggatatgct ggcattactg ctgttcctga
       61 catttaacgg tcatttatgg ttgatttcac tgctggtcga tacctttcac accctgccga
      121 ttggtagcga accgttgaac agcaatgcgt ttctggcacc caccaaagca gggagtttga
      181 ttttccttaa cgggctgatg ctggcgttac cgctcattac tctgttgctg accctgaatc
      241 tggcattagg tttactgaat cgtatggccc cgcaattatc catttttgtt attggatttc
      301 cattaactct gactgtcggc atctctttaa tggcggcatt aatgccgtta attgcgcctt
      361 tttgcgaaca tttattcagt gaaattttta atttgctggc tgatattatt agtgaattac
      421 cattaatata attccgtaac gtttatcatg ctaccctaag gattatccga aaaataatat
      481 ctgcgaacat cttccaggat actcctgtag cgaaatattt gttttaagct cactcacata
      541 tcgcaacatt tactttaaga caattccagg caaattatac aacactttac gggatagtaa
      601 gtccgcctga aaaattgcga gagtggcgca ttaggtgacc catgttgttc cgtttagtca
      661 tgatgaaata ttcaggtaag gggaattatc gttacgcatt gagtgagggt atgccatgtc
      721 aacgattatt atggatttat gtagttacac ccgactaggt ttaaccgggt atctgttgag
      781 tagaggggtt aaaaaaagag aaatcaacga cattgaaacc gttgatgacc ttgccatagc
      841 ttgtgattca cagcgtcctt cagtggtgtt tattaatgag gactgtttca tccacgatgc
      901 ttctaacagt cagcgcatca agcacatcat taatcaacat cccaatacat tatttatcgt
      961 ttttatggca attgccaatg ttcattttga tgaatatcta ttggtcagaa aaaatttatt
     1021 gaccagttct aaatcgatta aaccggaatc tctcgacgat atccttggcg atattctgaa
     1081 aaaagagaca acgataacct cgtttttaaa tatgccgacg ttatcattga gccgaaccga
     1141 atcgagtatg ttgcgaatgt ggatggcagg tcagggaacc attcaaatct ccgaccaaat
     1201 gaatatcaaa gccaagaccg tttcatcgca taaaggtaat attaaacgta agatcaaaac
     1261 gcataataaa caggttatct accatgtcgt ccgactgacg gataatgtga ctaatggtat
     1321 ttttgtcaac atgcgctaac acattctgac tggtggtttc ccaccagtca ggctgaataa
     1381 gattactctg ctttctcaac aaagataccg tcctgatgcc ctgcttcatt aaagaaccag
     1441 atgccgagcg ggtagtcttc cagcgcaacc aggtacattg tgccttcact aaactcctca
     1501 actgccagta ccactccagg acgacgcgga ccgccatccg ttttgactgt tacccgatca
     1561 ttcaccttca tcgttttcct cctgtggctt tgtgccagtg tagaacaatt tcgtttttct
     1621 ggcagcgcca agcgcgcgcg agtgctgatt ttctcgacgg tctatactta agagatgcca
     1681 ggcggactta acgactggcg gcaacaacag agtaacggtt gcgaggaaag atgatgaaaa
     1741 ccgcaaaagt gtacagcgat accgcaaaac gtgaggtcag cgtcgatgtc gatgccctgc
     1801 tggcggcgat caatgaaatt agcgaaagcg aagttcatcg cagccagaac gattctgaac
     1861 acgttagcgt cgatggacgt gaatatcata catggcgtga attggcggat gccttcgaac
     1921 tggatattca tgacttcagc gtctctgaag tgaatcgttg aatgcacaat aaaaaaatcc
     1981 cgaccctgag ggtgtcggga tgaaacttgc ttaagcaaga agcacttaaa aaattcgtta
     2041 caccaggaaa tctgatgtgt tcatcacctt atctgcaatt tttttcgctg acaagaaaat
     2101 attcgcgcta tgaatgatta acgctactta tgaatagaaa tgtgacgcgc gtcacccctt
     2161 ggtggctctg gtgtcaacgg gatcgctccc gaaaaaatca tctttgatta tttgtgctgt
     2221 tttgagaatc atcctggtaa ggggagtact gcgcgaccat gttttcaatt caacaaccac
     2281 tactggtttt tagcgatctt gatggcaccc tgctggacag tcatagttat gactggcaac
     2341 cggcagcccc ctggctcagc cgtttacacg aagccaatat tcccgtcatt ctctgtagta
     2401 gtaaaacttc agcggaaatg ctgtacttgc aaaaaatgtt ggggctacaa ggcttaccgc
     2461 tgattgcaga gaatggcgca gtgatccagc ttgctgaaca atggcaggat atagacggtt
     2521 ttccacgcat catctcaggt attagccatg gcgaaatctg ccaggtttta aatacgctac
     2581 gcgagaaaga acattttaaa ttcacgactt ttgatgatgt cgacgatgca accattgccg
     2641 aatggacggg attaagccgt agctaggcgg cgctgacgca gctgcatgag gcgtcggtaa
     2701 cgctaatctg gcgcgacagt gacgagcgta tggcacaatt tatcgctcgt ctgaacgaac
     2761 tgggcttgca gtttatgcag ggtgcgcgct tctggcacgt actggatgcc tctgccggaa
     2821 aagatcaggc tgccaactgg attatcgcga cctatcaaca attgtcaggc agacgcccaa
     2881 ccacacttgg cctgggcgat gggccaaacg atgcgccttt actggaggta atggattacg
     2941 cggtgattgt gaaagggcta aaccgtgaag gggtacatct gcatgatgag gatccggccc
     3001 gcgtctggcg aacgcagcgt gaaggaccgg aaggctggcg tgaagggctg gaccattttt
     3061 tctccgcccg ttaagcgtta tcgctcgcga atacccgatt gcgcccggcc tgttttgcga
     3121 ggtaaagccg acggtcggcc aatgactgga gttgttcaaa atcataatca ccggtttcct
     3181 cgctgctact tacccccagc gaggcactga tgcgtatcgt tgtactcttg gcgatcagca
     3241 tctctttttc atttaacttc aggcgaatac gttctgcgac ctccgcagcc tgcgtcagat
     3301 tcgcgcctgg cagaatcaca caaaactcct caccaccgac ccgcccggca acgtcctgcg
     3361 cacgcaagga actgctaatt aatccggcag catgagaaag aacacggtcg cccgcctgat
     3421 gaccaaagcg gtcattaatc gctttaaaat ggtcaagatc gacctgaatg acagaaaaag
     3481 gatgttggtg cgtctgacac aatttagcga gcggacgggc tttttcgaac agtgcgccac
     3541 ggttatataa acgcgttaag gtgtcgtgcc acgcctgcca ctgcaacgag ctttgcagaa
     3601 catacatgtt gctgaccatc cggcgaatca cataccagga aaggagtaac atggtggtaa
     3661 agagcgccca cagcagggtt aatgcaatgc tgatactgcc gaaatcgccg cgcacgcctt
     3721 cgcttagcgt atggacgcgc gccagcacac cgtcgaaatg gtccagacgt tcccagctaa
     3781 catagcgact gtccatacga atgccgcccc gcgtgtcatg ttccattgcc tgcgccagca
     3841 aggccagttc acgaggatca aaaatattcc ctgttggatg gtcaggattg gaagaggtca
     3901 aaaatatcag cttactgtca tagagctgat actcaccatc gaggttttta tcgatggcgt
     3961 ttcttaaaaa ttgctgcata gtacgcacgg gaatactcat ccccagcacg ccataccagt
     4021 agttattact gtctaccgga acactgacgg taacctgcgg ttcagtattg ctggcgtgtt
     4081 ccggttgcga agtgaaccag cgtaccgcgc ggtgacgatt ttctcgttgc gaatggccga
     4141 taaaccaggg ttgggtgaca tagccgtaat aacgcgttgg tacattgcgc ataaacaagg
     4201 tcggctgcgt cgaaacgtaa aatccagcac gcgagacata catcgcctgt tcaaccatcg
     4261 acgaggagtt gtgcgccagt cgcagcaagt aaccaacttc cagtgcagcg gtaatttcat
     4321 tgtcgaggct ttcattttcg cgagacagga aattcccctc gctgactaat gcatccgata
     4381 cgccattgac tggcagggtg cgccgtcggt tgagttcgat ttgccaggcg tgctcgtcgc
     4441 gatgctgttc gaactcggtt acggcattac gcagagaggt gaaatccagt ggcgccacga
     4501 gagcttcgcg catgccatta cgcaaaaaga tcagtttgtc gacgttatac tgcaaatgct
     4561 tatcgagcgc gttggctacg ttttccagat gattacgctg gctggagata taggcatctt
     4621 ccagcaccac cacttcacgc caggtgagca aggtggaaaa aagcaatacc acgataaagc
     4681 agagattaac gacatgaccg ggccccaggc ggcgtgcgag ttttttcaac cagctctggt
     4741 tttccatttt tgtctcgtgc tgcaccctga cgactcccaa cctgcttttt tatgattctg
     4801 gcataacccg gcaatagcg
//
LOCUS       DADMFL010000200         4819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.206, whole genome shotgun sequence.
ACCESSION   DADMFL010000200 DADMFL010000000
VERSION     DADMFL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.206"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            94..429
                     /locus_tag="JNZ27_003982"
     CDS             94..429
                     /locus_tag="JNZ27_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666097.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
     gene            603..821
                     /locus_tag="JNZ27_003983"
     CDS             603..821
                     /locus_tag="JNZ27_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666098.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            1024..1236
                     /locus_tag="JNZ27_003984"
     CDS             1024..1236
                     /locus_tag="JNZ27_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8666099.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1278..1463)
                     /locus_tag="JNZ27_003985"
     CDS             complement(1278..1463)
                     /locus_tag="JNZ27_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666100.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            1404..1682
                     /locus_tag="JNZ27_003986"
     CDS             1404..1682
                     /locus_tag="JNZ27_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666101.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            1756..2742
                     /locus_tag="JNZ27_003987"
     CDS             1756..2742
                     /locus_tag="JNZ27_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8666102.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVS
                     IAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2743..3108
                     /locus_tag="JNZ27_003988"
     CDS             2743..3108
                     /locus_tag="JNZ27_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8666103.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3105..3794
                     /locus_tag="JNZ27_003989"
                     /pseudo
     CDS             3105..3794
                     /locus_tag="JNZ27_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
     gene            3988..4063
                     /locus_tag="JNZ27_003990"
     tRNA            3988..4063
                     /locus_tag="JNZ27_003990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4021..4023,aa:Ile,seq:cat)
     gene            4065..4141
                     /locus_tag="JNZ27_003991"
     tRNA            4065..4141
                     /locus_tag="JNZ27_003991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4099..4101,aa:Lys,seq:ttt)
     gene            4144..4219
                     /locus_tag="JNZ27_003992"
     tRNA            4144..4219
                     /locus_tag="JNZ27_003992"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4177..4179,aa:Thr,seq:tgt)
     gene            4223..4297
                     /locus_tag="JNZ27_003993"
     tRNA            4223..4297
                     /locus_tag="JNZ27_003993"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4256..4258,aa:Gly,seq:tcc)
     gene            4590..4805
                     /locus_tag="JNZ27_003994"
     CDS             4590..4805
                     /locus_tag="JNZ27_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8666104.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1279 a         1076 c         1273 g         1191 t
ORIGIN      
        1 gcggtaatga aaatgactga attaacaaaa gaacaattaa tcgaagaagc caaattaaaa
       61 atagcgatta cgaaatgcca ccccaattca gggatggcgc gagtagaggg cgagttattc
      121 aaaattgcac gggcatcgct ggaagcagag ccgatagcgt ggcgatatcg ctacgtgaaa
      181 aaaggtgtta tggactctca gggggagttg tgggttggtg actggaaata tgtaccgaaa
      241 aaagaggatt gtaacgacag gccgaactat gaaattcagg ccttattcac tgccccacca
      301 gtcccggtta catcagaaga actggttaaa gctgtgcact tttatgaaca actaaaacgc
      361 gaaaatccac cagcatccgg caaccagatt aatgggttaa ctatgtcggt taaacgacca
      421 gccaactgaa aaagcggaaa cctgattata ggttgccaga taaggcaata agctacctgg
      481 cgcggaacgg actgataagt atggggaatg ttttacgatg agtatttaga ctaaagagtt
      541 tgtaacgcta tgtaagtgat tttttctggt ttagatattt atatgtccgg ccaaattgag
      601 gtgtgtttaa atgttattgc acattgattg tagggggaat aatgaaaaac gcattgcagt
      661 ttttgtttgt tgcgttctgg ttgttcgcat catgtatgcc catcatcttc acagcaaggt
      721 atatggaaaa aattgatgtt ttgatattaa tgtttggaca tataaatgcc ctttttttag
      781 gggtgttcat ggcggtcatg tgcattgaat actggcggta aatacagcga acgctattgg
      841 tttagttgga tatttactgt gccggacaaa aacggtttgc agggaaatct tagttaagta
      901 gaatgactgc gggtgcttga ggctatctgt ctcaggcatg aacaccaaaa ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc cttatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag
     1201 gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg gagaaatccc
     1261 tcgccaccgc tgatgtgtca ggcatcctca acgcacccgc acttaacccg cttcggcggg
     1321 ttttgttttt tctggtcgtt ctggtttaca atccatccgt cagcctgaac aactggcacc
     1381 tgctgcgcca gcagagaaaa cagatggcgc acgataccaa attttacaat tcggataact
     1441 ctgccgcccc tgccagcagg cacgggcggc gttctcatgc attcaaatct gactggtatc
     1501 agcacgaccc ctgcaccgaa gaacaggctg aatggctgat tcagcgctac cgcagacacg
     1561 gatacgagat taagaaagcc ctcagtctcg attatcgtca ctggataatc ttcgtcaggc
     1621 tcccttattc cgaacggcca ccgcgtccgt cctgcacatt ccagcaacgg atctggaggt
     1681 aatgtgcggg tattacttcg acctgttctg gttccggaac tcgggctggt ggtccttaag
     1741 ccaggccgtg aatccatgcc ggtattccac aatacccggg tactggtgga gccggaaccg
     1801 aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg ttcgccagcc gctggtggaa
     1861 gacaaaacat tgctgccgtt tttcagtaac gcacgggtga ttcgtgctgc tggtggtgct
     1921 ggcgcattgt ctgactggct gttgcgccat atgaaatcct gccagtggcc acacggcgat
     1981 tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg gcgcaatggt gttgtgctgg
     2041 cactgcgaca accagttgcg tgaccagaca tccgaatcac tcgagcaact tgctcatcaa
     2101 aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac gcaggagcgt
     2161 gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc ggacgcgcta
     2221 ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg ctcaatgtac
     2281 cgtgaaagcg acatcgtgcc gggagagcag accgccacca gcatactgaa gcagcgcaca
     2341 aaaaatcttg cgccgctgcc tcacgcccac cagcaacaga atccaccaca ggaagagacg
     2401 gtggtcagca ttgccgttga tccggaatct ccggctcagt atctccagcg ccagaaacca
     2461 caacgggaag agatgcctgt atacacgcgc tgggtaaaaa cgcagaaatg catgacgtgt
     2521 ggcaatcagg cagatgatcc gcaccacatc attggtcatg gactgggagg gatgggaaca
     2581 aaggctgacg atttgtttgt tattccgctg tgtcgtaaat gccataacga actacacgcc
     2641 ggggtaaaag attttgaaga aaaacacggt agtcagttgt tgttgctgat tcgtttttta
     2701 atgcacgcga gaaattcggg tgttctgaag tggaaagcat aaatgaccga acgcatagaa
     2761 tttgttttgc cttacccgcc aacggtgaac acctactggc gacgtcgtgg cagcacatat
     2821 tttgtatcaa aagccggtga gcgttatcgc cgtgatgtgg cgctaattgt tcgccagcag
     2881 cgactgaaat taaacctgtc cggaaggctg gcgataaaga ttattgcaga gccaccggat
     2941 aagcgccgtc gtgacctaga caatatcctg aaggcaccgc tggatgcgct gacgcatgct
     3001 ggactactta tagacgacga gcagtttgat gaaatcaata ttgtgcgcgg tcagctcgtt
     3061 cctggtgggc ggctggggat aaaaatcaca gaactggggt gcgcatgaat aaccagtatt
     3121 tacagtttgt gcgtgagcag ctcattatcg ccaccgctga tttgagtggg gcaacaaaag
     3181 gtcagcttga agcctggcaa gagaatgcca tgttcgatac agggcgttac aggcgtaaaa
     3241 aaatccggta ccgcgatgaa gtgactggaa aaatgataac gcgggataat ccaccaatcc
     3301 cgggaaagca atcgctggcg aaggggacgt caattcctct ggtcagtccg gttgagtttt
     3361 cgacatcatc gtggcggcgg gctgttctgt ctcttgaaga acatcataaa gcctggttgt
     3421 tgtggtgtta cagcgggagt atttgttggg aatatcagat cgcgataaca cagtgggcgt
     3481 ggaatgaatt taatgctcaa tccggtacca gaaaaattgc agggaaaacg caggaacgcc
     3541 tgaaaaaatt aatctggctg gcggcgcagg cagtaaaagc agaacttttt ggtggggaag
     3601 gttatgaata ccaggagctg gcatttactg gcgggagtga caactaaaaa ctggtccaaa
     3661 acatttactc gtcactgggt tgcaatgaaa cacatttttc accgactgga tagtgaggct
     3721 ttattgtttg taatgagaac acgttcaaaa aaaagacggc attttcaaag caaagtgttg
     3781 caaaagtaga ttgaaaggca tatattcatg caaatctgat attttgccga ttttgtacgt
     3841 gatggcaaaa gcaaacaaaa cccgcccaca agcgggtttt ttgtgccact tatctcggat
     3901 agacatggtg aatgcgctgg tggaggagat aagggtgatt tttgaatgct tgcaacattg
     3961 atttcgtaac gttattatcc tgcgcccggc cctttagctc agtggtgaga gcgagcgact
     4021 cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca ctagctcatc
     4081 aggaaagagc gtcaaccctt taagttgagt gtgcgaggtt cgagtccccg gtggcggtcc
     4141 agtgccgact tggctcagta ggtagagcaa ctgacttgta atcagtaggt caccagttcg
     4201 attccggtag tcggcaccat atgcgggcat cgtataatgg ctattacctc agccttccaa
     4261 gctgatgatg cgggttcgat tcccgctgcc cgctccagtt agagtctttc agtctgcgat
     4321 gatgggaaat cccggagtga ctgaaagacg tttaagttat gaatgatcgc ttttttttgc
     4381 aaaattgctg tgcagaaata ctaaccttcg ggcaggcgat cattcataag cactctgctt
     4441 ttattccgat taactgtgag tggtttgttg gatagagtgc tttccttact gtatatatcg
     4501 tttcgcccgc ttttgcggtt ttttcttttc aaatcccttt catttctcag tgtaaaacta
     4561 cgccatccgt tatttgcgga ggtgaggcta tgaaatccat ggacaaaatt tcaacgggca
     4621 ttgcctacgg cacctccgca ggcagtgctg gctactggtt tttacagtgg cttgatcagg
     4681 tcagtccgtc acagtgggct gcgattggtg tactggggag tctggttctg ggcttcctga
     4741 cttatctgac aaatctgtac ttcaaaatca gagaagacaa gcgtaaggct gcgagaggtg
     4801 aataatgtcg ccatcatta
//
LOCUS       DADMFL010000201         4817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.207, whole genome shotgun sequence.
ACCESSION   DADMFL010000201 DADMFL010000000
VERSION     DADMFL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.207"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..1103)
                     /locus_tag="JNZ27_003995"
     CDS             complement(<1..1103)
                     /locus_tag="JNZ27_003995"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8666105.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
     gene            complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JNZ27_003996"
     CDS             complement(1314..2711)
                     /gene="ccp"
                     /locus_tag="JNZ27_003996"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY8666106.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3116..4765
                     /gene="treF"
                     /locus_tag="JNZ27_003997"
     CDS             3116..4765
                     /gene="treF"
                     /locus_tag="JNZ27_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY8666107.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1219 a         1242 c         1157 g         1199 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttgtgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt gttttcgtca caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgtac cggaccgcac actaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatatt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt attgatcgac aaatccatca atttatggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggctaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctggaatgc gacatcatcg cgcatttcgt gcagcggaaa tcgttttgac
     1021 tccgcgatag tagaaatggc ctttgcgcca aaacgtgaat cgagtagttc tgagcggaaa
     1081 tccgttaaca gcttctggtc catttcgaac tccttaaatt tatttgaagg caataaaaaa
     1141 gtaggattta tccgcaatgg aagcaaggca ttacaggctt aatttaaata acaaaatcct
     1201 aagcagaaaa acgacttttt ataatataaa catttaacat gataatatta aaaacagact
     1261 ttattatcgt aataaaaatc gcccaaacat tcctgttcgg gcgatcaaat taattattgt
     1321 ttatcctgca tatacggcgt gtacaccccg ttcagactgt gcaggaaagc tacgatatca
     1381 tccacatcct cctgcggcag ctctttgcct acctgatagc gcagcatcag tttcaccgcc
     1441 ccgtccagcg tcggcacgtc accgcgatgg aagtacggtg ccgttaaagc aacgttacgt
     1501 aaaccgggta ctttctgacg caatttatca cgctcttctt tagtcacatt catacgacca
     1561 atatccgccg ccgtaatttc cccaaagtta aagtcttttt tcagcaccaa cggttcaaag
     1621 gaacgtccgc cgagaataat accaccatga caagttgcac atttattatc tttaaataat
     1681 tgatagcctt ttttctgttg cgccgtcagc gcattttcat ctccacgcaa ccatttatca
     1741 aatggggaat ccggcgtaat taatgttttc tcaaattcag caatggcatc agtaatattt
     1801 tcgccactga aaccttgcgg ataaacttcg aggaactgcg ctttaagctg cggatctttt
     1861 tccagcttag caataatttc gtcccaggat ttcgacgcca tttcaatcgg gttcaacggc
     1921 ggtccacctg cctgatcctg caatgttgcc gcacgaccat cccagaactg ctcaacgtta
     1981 aatactgagt taaataccgt cggcgcatta atcggcccaa ctgcgccacc aacaccaatc
     2041 gatgtttttc tgccatcgac gccccccgca ttcaacgtat ggcaatgagc gcatgaaatg
     2101 gtgctatcag ccgataaacg gggatcgtga tacagcgcaa agcccaacgc cactttttgc
     2161 gcatcggtag gcagtttttg cgggatgggc tgcaccggtt cattgcgatg ctccggagca
     2221 gtatcattgc tggcgtaata ttccgcgcgc tgttttgcaa tccaggccag tatttccgcc
     2281 cgctcttcat cactcacctt acccgcccag tgtagcgcgg tataacgcgt tggtggcata
     2341 gtttcatact gcatcaccca ttcaatctta ttcagatcgc tttgcgaaac gggtttatca
     2401 gccagcagtg ccgcacgcac ggcctcgagg ttaaaagatt tatatccaag cttaatgtcg
     2461 taatccatca actgtttcgc gccaggaata taataatagg cgggtaattc tgccgaaggc
     2521 gtgtggcaat agtcgcatcc tttttcgcgg agaaagccta aaaccttatt attttcactg
     2581 acagcagatg cctgaacatc ggcctgttta ctgcgtttat tatcgtggta ccacacataa
     2641 ccagataacc ctaaatagca aatcgcgacg ccagccaggc cgatcgcggt aatacgtgag
     2701 accattttca ttattcttcc ctcacggtgg tgagttattg tgacccagaa aattttccag
     2761 aatgctgcaa acgtctctgc gagacaaagc catcataaaa aacagggaga tacgcttttt
     2821 gataggaatc aatcaattac atgaatataa actatcaata agatagcctg tgcctattac
     2881 ccagatcagg aatggcatat catgattgcg gcgagagcag gattgagtgt tctgaaaatg
     2941 aaaaacaaca ccaaatccgt atttttataa attccattga tattagtgcg ttatattttt
     3001 tgacaagtta ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga
     3061 ctacccttaa agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct
     3121 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta
     3181 tgaactggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     3241 gatgattgaa gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     3301 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     3361 acccaaaatg gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     3421 ttttgacttg cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga
     3481 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     3541 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata
     3601 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     3661 gggactggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     3721 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     3781 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     3841 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     3901 gttgatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     3961 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     4021 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggagcggc
     4081 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     4141 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     4201 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcgctgttcc gccagaaggc
     4261 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     4321 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccgct
     4381 ctatgtcggc atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tacgcagccg
     4441 gttactgaca cctggcggga ttttggcaag cgagtacgaa accggtgaac agtgggataa
     4501 acccaacggc tgggcaccgt tacaatggat ggcaattcag ggatttaaaa tgtatggcga
     4561 tgaccttctg ggtgatgaaa tcgcgcgcag ctggctgaaa acggtgaatc agttctatct
     4621 ggaacagcac aaaatgatcg agaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     4681 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     4741 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     4801 gtaaaattat cctcttc
//
LOCUS       DADMFL010000202         4681 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.208, whole genome shotgun sequence.
ACCESSION   DADMFL010000202 DADMFL010000000
VERSION     DADMFL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.208"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..213
                     /locus_tag="JNZ27_003998"
     CDS             <1..213
                     /locus_tag="JNZ27_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8666108.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JNZ27_003999"
                     /pseudo
     CDS             356..577
                     /locus_tag="JNZ27_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JNZ27_004000"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JNZ27_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JNZ27_004001"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JNZ27_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY8666109.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JNZ27_004002"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JNZ27_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JNZ27_004003"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JNZ27_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY8666110.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1629 a          840 c          873 g         1339 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagctg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggagatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg cgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acatcccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatggctct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
     4681 g
//
LOCUS       DADMFL010000203         4612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.209, whole genome shotgun sequence.
ACCESSION   DADMFL010000203 DADMFL010000000
VERSION     DADMFL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.209"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1347
                     /gene="tnaA"
                     /locus_tag="JNZ27_004004"
     CDS             <1..1347
                     /gene="tnaA"
                     /locus_tag="JNZ27_004004"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY8666111.1"
                     /translation="RAYREEAIIKSGMNPFLLDSEDVFIDLLTDSGTGAVTQSMQAAM
                     MRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDR
                     SKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGIEEV
                     GPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQR
                     EAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCV
                     VQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGG
                     HAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPA
                     ELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            1438..2685
                     /gene="tnaB"
                     /locus_tag="JNZ27_004005"
     CDS             1438..2685
                     /gene="tnaB"
                     /locus_tag="JNZ27_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY8666112.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2817..3992
                     /gene="mdtL"
                     /locus_tag="JNZ27_004006"
     CDS             2817..3992
                     /gene="mdtL"
                     /locus_tag="JNZ27_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY8666113.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3967..>4612
                     /gene="yidZ"
                     /locus_tag="JNZ27_004007"
     CDS             3967..>4612
                     /gene="yidZ"
                     /locus_tag="JNZ27_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY8666114.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1019 a         1138 c         1181 g         1274 t
ORIGIN      
        1 cgcgcttatc gtgaagaagc aattattaaa tccggtatga acccgttcct gctggatagc
       61 gaagatgtgt ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg
      121 caggcagcga tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta
      181 gccgagtcag tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt
      241 ggcgcagagc aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg
      301 gatcgcagca aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc
      361 cagattaacg gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg
      421 cgttacgact ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt
      481 ggcccgaata acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag
      541 ccggtttcac tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg
      601 gtggtaatgg actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca
      661 gaatacaaag actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg
      721 ctggcgatgt ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa
      781 gacgacagct tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc
      841 ttcccgacat atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat
      901 gacggcatga atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat
      961 ggtctggaag agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat
     1021 gccggtaaac tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc
     1081 gagctgtata aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc
     1141 gatccgaaaa ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg
     1201 cgcgcaacat atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa
     1261 gagaacgcgg cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac
     1321 ttcaccgcaa aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc
     1381 cactctctta ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg
     1441 actgatcaag ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca
     1501 gtaattggtg gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg
     1561 ggtgccttta tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta
     1621 gaagcaaatt taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata
     1681 ggtaacacct ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact
     1741 tatgcctata tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc
     1801 tatcacgcta atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg
     1861 tggataagtt cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc
     1921 tcctttgtga tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat
     1981 gccaccagca ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg
     2041 tgtctggcgt catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa
     2101 cgcaaagata agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat
     2161 ctcttctggc tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc
     2221 tcctcaggcg gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc
     2281 attatcgagt tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt
     2341 gtcacgctgg ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc
     2401 gggcgtttca aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc
     2461 ccgaacggct ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc
     2521 attattcccg cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg
     2581 gtctggggcg gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg
     2641 tgctggttcg gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc
     2701 agccattcgc tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct
     2761 tatcctcctt caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt
     2821 cccgcttttt gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt
     2881 acctcgttgg tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata
     2941 ttgcgttctc cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg
     3001 ccgatcgttc agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct
     3061 cggtgttctg ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg
     3121 ggttgggcgc aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg
     3181 atcgacgtcg ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt
     3241 tagcgccagt gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg
     3301 cgatggcaat gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc
     3361 gcccagcgtc ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc
     3421 gttttttcct cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg
     3481 tcaatacatc gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca
     3541 ccattatggc gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc
     3601 tgggaatttt taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg
     3661 ggatcaccct tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt
     3721 gcgccggttt ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat
     3781 tacgcgcggg cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt
     3841 ggatttggct ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga
     3901 ttgcctgtag catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg
     3961 ccgctcatga agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg
     4021 ctgatgcagg agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg
     4081 gtgagtaagt cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca
     4141 ccgctggggc tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg
     4201 atgcaaatga gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag
     4261 tttgagttgg cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg
     4321 atctaccaac gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta
     4381 gatgccatta ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc
     4441 tcgcgggagc tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat
     4501 gtaccctgcg tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac
     4561 acctttttac gttatccgca tatcagcatt tgctgggaac agagcgacac ct
//
LOCUS       DADMFL010000204         4577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.210, whole genome shotgun sequence.
ACCESSION   DADMFL010000204 DADMFL010000000
VERSION     DADMFL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.210"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            437..2089
                     /locus_tag="JNZ27_004008"
     CDS             437..2089
                     /locus_tag="JNZ27_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8666115.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="JNZ27_004009"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="JNZ27_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2349..2462)
                     /locus_tag="JNZ27_004010"
                     /pseudo
     CDS             complement(2349..2462)
                     /locus_tag="JNZ27_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..2922)
                     /locus_tag="JNZ27_004011"
     CDS             complement(2596..2922)
                     /locus_tag="JNZ27_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8666116.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(3207..3859)
                     /locus_tag="JNZ27_004012"
                     /pseudo
     CDS             complement(3207..3859)
                     /locus_tag="JNZ27_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT         1218 a         1107 c         1056 g         1196 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtatgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagaaagg agaaactcgt
     3001 agcgacgttt ctccttttaa acgtgtcaat gtaggcgata ttcgtcttcc ctgcgttcag
     3061 gaccaacccg acttccgaga agcgcaccga taacctgttc ttcagccttg ttgcatcact
     3121 cattcgtgaa caatgtacta caagtcgagt cacccgaggg aacctccccc tctggtgctc
     3181 acggaaccgg acgtgaacct ctcagctcat ccggctcttc tcattgaggc ctgtccccat
     3241 agtaacggcc agtgtgcaaa taacctcgtt tcactccgcg caacacgggc cagccagtgg
     3301 ctggcccgtc gtcggtggcc cctaagtcgc ttgtgtttac ggggcagcca taacacgagt
     3361 ttgcgatcga tttgcctcag cgtcagatac agtgctgatt tataaaaagc accgtagtac
     3421 tttatccagc ctctgatttt cgcattgaac atatgcgcca gctcgtacaa ggacttgtca
     3481 ctgcgattct cctcacctcc tgacggattg cttttcctgc tctggcactc atcgccggac
     3541 tgaagttaac gaaggtctta ccccagcgat tcatcgacag acgcggccgg aatgtatagc
     3601 ccagaaagtt gaagctcgtt tccgggtagt ccccccgtcg gtcagcatcc ttacagtaaa
     3661 ctattcgtgt tttttgcgga tgcagctgca gcccgcattg cgcaagccgc tgcctcagtc
     3721 cacttatcag agcgtcagcc tgccactggc tgtgacagtg gcatacaaca tcatcggcgt
     3781 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3841 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3901 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3961 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     4021 catcaaagaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     4081 tcgcctgcgc aatcgcctgg tgtgaggatt tacccggtcg ataaccatac gagtctgcat
     4141 ggaacagggg gtccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     4201 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     4261 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     4321 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     4381 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     4441 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     4501 cagcacaatg gggcaagccc tttgcttcgg acccattacg gtccttcacc gctactacgg
     4561 tttactccgt ccctgta
//
LOCUS       DADMFL010000205         4566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.211, whole genome shotgun sequence.
ACCESSION   DADMFL010000205 DADMFL010000000
VERSION     DADMFL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.211"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(162..4256)
                     /gene="sepA"
                     /locus_tag="JNZ27_004013"
     CDS             complement(162..4256)
                     /gene="sepA"
                     /locus_tag="JNZ27_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY8666117.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1107 a         1173 c         1014 g         1272 t
ORIGIN      
        1 ctgaaggggc ggccagtata gttggcgcca gtcggcaaaa ctagttgacg tctaatacct
       61 ggacggtcac ttctgagaaa aggcatttac acagaatctt aaacaggctc acggagttct
      121 ttcagaaaaa ggcctgccga atggcaggcc tatcccattc gtcagaaaac ataacggaag
      181 ttggcgttta tcgcattatc cacattatat ttcccgaacg ctgatttctc cagctccagt
      241 cccaaacgca tattatcctt aatttccgca ttcatcccta ccgtcatcag catcctgcta
      301 tctttttcac cttcgaaacg tttctctccg gaagcatcct gcagaaccgt ttctccgttc
      361 gccagcaggt cgaactgata acccagcccg gctcgagctg tgattttcca gtcgtctccg
      421 gagaaggctc ttcccacgtc aacaccagta cggccaatca gtgggttata atccttgtct
      481 ttcatgctca gagccattcc ccggtcttcc cagctaaaag cttttcctga tacagaaccg
      541 taaaccagct ctatctgtgg ctccacccag gactctttcg tcaggtggta acgataacca
      601 acttcagcac cggtatacca ggaatgagag ctgtagtctt ttgttcccag tgaggcaaaa
      661 ttcgccgtgt gctgattatc atgatggaga tatttaccaa tcaggtcaaa ataagctccg
      721 gaattaaaga gtgcagaggc atacagaccg ccacccacgg atttgttttt tccactgaat
      781 gcgtggctgc ttgcattgct gtccgtatag gtcagcaatg cccccgtaaa taaatccaca
      841 ccgtccagct catgctttct gtcgacacca atctgaacgt gagtgtagtt atcgctgtag
      901 tcaccatctg ccgaaccggt accattcatt atgcgtgccc agacaccggc atccccctga
      961 gtatcccgca ggtcacccat acgtttgttc aggttattga cctctgtaag gaaggtctta
     1021 taacctgatg ccataaagtc ttttgcggct ttcgaggctc cggcatcagc ggtagtctga
     1081 tatcctgtca gcacccattt tgtggcatca tccgtttttt ccgtgctgat tactggcgta
     1141 acgttactga aaccaatctg ctgcgtaccg gcagtgaacg ttttctcatc actccctttc
     1201 ggtgcggtaa ttaacgtcac gctcatttca ctggctggtt tgtttaaaaa gtcaaccagg
     1261 acgctattat tactgcctga cagtttgtct gtgacattca actggtcagc ccggctgttg
     1321 ttggtatgca tcaggaacgt ggtattatct gcagaaagct cttttacttg taaagtggca
     1381 aagtgtttcc cgtcccccag ttcaattcgc gagcccttag ccttcagtgc accggcctct
     1441 gattttccgt cagagtacca cagagcattg atcatattta ccgtactctg tgcccccgta
     1501 atactgcctc tcagaacagc gttatatccc ttcattaagg agctgaataa cggagagtcc
     1561 gtcttccctg catcagcatc actgtctccc agattaatgg ttgccgcagt ggttcctgca
     1621 tgaatatccg aactcagata gcccatattt ttaacagaaa agctggcgtt atcttcgagg
     1681 ttaatccctt ctgttgtaga aattacgggt gagtaatacc cctgtttctg cgcagaaggc
     1741 atccccgtca gggtcagaga agcattttct tttacatcca cagccccggt gctgaaaata
     1801 ccgctctgag ccgtaagttt tgctcctttt ccgagggtca ggctgctgtt gacaaagctg
     1861 ccaacacggt caaaaacagc attctgagag gtcacactca cggtgctatc ataagcctca
     1921 ataccaccgg tgaatctctc catgatttgc agggatgatt tattttccag cgtaacattt
     1981 ccgctgtaat cactctggtc accaactgtg gttgcagtgg cactcccttc aacagaggag
     2041 accgcattac cggttccgtc actcctgtca gtaaagacac ggctacttcc cagaatgact
     2101 gtacttccat tggtggcgtt aatatctccc tgcatcttgc cgtttctgga cagatggaat
     2161 gttgccgcat cgagtttcag tttttccagc gtaaactgac gggtttccca gtcgctctgg
     2221 ctggaagaag tcgtcgttcc agcatgaata actggatgcc cctggaatat cagcgtgccg
     2281 gacttcttgg ttatcgaacc ttctggcagg ttaactgaac catcaagagc cagtacatcc
     2341 ttacctgaga gctgcggaat attcacatcc atattgccgt ttagttggcc gtgatacata
     2401 taaggctggc tgctggcctc aattttttgt tgcttcacag tatcaatggc tttattacga
     2461 tcttttccga cattctgcca gacactgctg ttgttcagat cggagaagaa cggggaatat
     2521 gaactcgctt tcaggatgaa atattgcttg gtagaactgt cataatatag gtctcccggc
     2581 gctcctctcc ccccaaaaat actgacagta ttaaccggta cattgatgtc actggctttt
     2641 aatgtctgaa ggtcgagtgt taccgttgat tttttctcac tgttattggt aatgatggcc
     2701 ccgtagtcag ccgcctgaag acgggtaaag gtcagattat taccattcag ttccagacgt
     2761 cctccacggt atccccacga aatattatcc ggattaactt gctgtgagtc agaaagcaca
     2821 actgtcggac gaccgctggc aataccaaca gagctgaaag cctgtacctt tccgtcagca
     2881 tccgcctgct ggttgagaat aacggtaccg tcccccacct tgagtccgcc tgcattcaca
     2941 ccggtcccgt ttactgtcag ggtaccttcg ccagttttat gcaggttatc accggcaact
     3001 ccgttcacct gccacagaac gtgggttcct ttatcggtga taatcccgcc ccccatccat
     3061 gtctggccgt tcagggcgga caccctgtaa tcgccggcaa actgcagata cccagctccc
     3121 tgattaacgg aattctgcag aacgacctcg ccattattgc cggtgaacag gaggtttttt
     3181 cctgcgttca gatcatttcc tttctttcca tgcatatccc aagtcgtgtt tccctgagtc
     3241 agtgtgccgg tgccattagc cgcatcatat ttccactgca gtactccttc accagaggtg
     3301 taggctatag ttttgtcaaa atcattttgc ggttgctgac caaggaaatc ctgagtggtg
     3361 acgacccagt tatttccata gttagttcct gaggaaagca cgccaatcag tacccatttt
     3421 ttttgcaggg aatcataagc aaacaggggg gagccgctgt cccccatatt cagataatta
     3481 gccagaggtc cattcgcggg attaaaaatg tcaccggtct gagctgtaat catctgtccg
     3541 ttgttatagg agttcagcgc gctgacggtt cccccaacaa gaaatccgcc attggtatac
     3601 acttgggtgc gattaccatt tttgtctttg acgtactgca tccccccccc aaggcgatag
     3661 aattcagtaa agcgtccgcc ggcctgatag gcaccgctca cagccccgat gtcagacact
     3721 tctgccggcg ccacctctgt caccagttta ctcaggcggc gggttttaat atcaagaccg
     3781 ctattattat ttgtaccaac cgcagtatag gtgtttttgg cgtatccgaa gctcatcgtg
     3841 tcagaaccgc tgacatgttt ggcggtaacc acatactgag gggagtaaag tgtatggtct
     3901 cccggaggca ggctaccggt agtaatagtg gcactactga aatcagccat cggggcttta
     3961 tcaagtttac cgaccagatt gccctgcttg tcgtaaatgg aaatattcgt ggtccccgac
     4021 gtaaactgac ctttattctc ggcaaagtcg cggaatatct gataagggat ctctgcactg
     4081 accgttgctg acagagctgg ccatgccgaa gaaagtgata aagctgcaac agacgtaaga
     4141 ataacccgac gtgaaagtct gcgatggctt ttgcaggtaa ccctgcgggc cagttcggag
     4201 acagcaatca ggcttttggt tatatggcaa tacttaagat aatatatttt gttcatatag
     4261 aaacatcaac ctaattttga caaagattaa ttacatagga tgtagatgga gcagtccgag
     4321 attctactaa tatcaattat taagcatctg ttttttagtt ggtgtaatat aatttttatt
     4381 aaggtaagcg gaaaaacaac caaaaaaaac gcaagatgtt atttttttat cattttgagc
     4441 cagtttggct ggggggatgc tcttctctta agcttcttgc tcaactttaa aaatagttat
     4501 gccagttcgg tatcgataag gggcagtttg gatatcgaga accacggcag cctgacggct
     4561 gcaaat
//
LOCUS       DADMFL010000206         4550 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.212, whole genome shotgun sequence.
ACCESSION   DADMFL010000206 DADMFL010000000
VERSION     DADMFL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.212"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..81
                     /locus_tag="JNZ27_004014"
                     /pseudo
     CDS             1..81
                     /locus_tag="JNZ27_004014"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            316..1224
                     /locus_tag="JNZ27_004015"
                     /pseudo
     CDS             316..1224
                     /locus_tag="JNZ27_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
     gene            1224..1958
                     /locus_tag="JNZ27_004016"
     CDS             1224..1958
                     /locus_tag="JNZ27_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8666118.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2426..3187
                     /locus_tag="JNZ27_004017"
     CDS             2426..3187
                     /locus_tag="JNZ27_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8666119.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGIPPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFTSHLQS"
     gene            3231..4310
                     /locus_tag="JNZ27_004018"
     CDS             3231..4310
                     /locus_tag="JNZ27_004018"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8666120.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMGYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQHFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="JNZ27_004019"
     CDS             4307..>4550
                     /locus_tag="JNZ27_004019"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8666121.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          869 c         1119 g         1315 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtgaccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagtgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttacaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tctgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt ccgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttacat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgactc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggggt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagcatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa agcgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DADMFL010000207         4549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.213, whole genome shotgun sequence.
ACCESSION   DADMFL010000207 DADMFL010000000
VERSION     DADMFL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.213"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            132..575
                     /locus_tag="JNZ27_004020"
     CDS             132..575
                     /locus_tag="JNZ27_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8666122.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /locus_tag="JNZ27_004021"
                     /pseudo
     CDS             811..1630
                     /locus_tag="JNZ27_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNZ27_004022"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JNZ27_004022"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY8666123.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNZ27_004023"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JNZ27_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY8666124.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNZ27_004024"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JNZ27_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8666125.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAAVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /locus_tag="JNZ27_004025"
     CDS             complement(4321..4470)
                     /locus_tag="JNZ27_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY8666126.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1234 a         1189 c         1083 g         1043 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 actacttgta atcaattgat tataaaggat taattcctaa aaagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag atcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acagcagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagggtaatg ctgccaactt actgattta
//
LOCUS       DADMFL010000208         4441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.214, whole genome shotgun sequence.
ACCESSION   DADMFL010000208 DADMFL010000000
VERSION     DADMFL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.214"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..214)
                     /locus_tag="JNZ27_004026"
     CDS             complement(<1..214)
                     /locus_tag="JNZ27_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY8666127.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            524..2296
                     /gene="aspS"
                     /locus_tag="JNZ27_004027"
     CDS             524..2296
                     /gene="aspS"
                     /locus_tag="JNZ27_004027"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY8666128.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2414..2866
                     /gene="nudB"
                     /locus_tag="JNZ27_004028"
     CDS             2414..2866
                     /gene="nudB"
                     /locus_tag="JNZ27_004028"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY8666129.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2895..3635
                     /locus_tag="JNZ27_004029"
     CDS             2895..3635
                     /locus_tag="JNZ27_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8666130.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3670..4191
                     /gene="ruvC"
                     /locus_tag="JNZ27_004030"
     CDS             3670..4191
                     /gene="ruvC"
                     /locus_tag="JNZ27_004030"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY8666131.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4193..>4441)
                     /locus_tag="JNZ27_004031"
     CDS             complement(4193..>4441)
                     /locus_tag="JNZ27_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666132.1"
                     /translation="SKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGS
                     IPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1101 a         1107 c         1232 g         1001 t
ORIGIN      
        1 catcaaccgg ctgttttaat gcttcggcgt aatcggcaga ccagccaact cgcaccagaa
       61 acacgggctg accgctggcg cgaaatttcg ccgccagctt cccggcgcga ttaactacct
      121 catcggcagt atgtggacca ccggcaaaag gtaagatgcc ttcttgtaaa tcgatcacca
      181 ccaacgcagt ggttttagca ttaagttcaa gcatgctgac tcctgaaaaa acaaaatcgt
      241 gcctcacacc ttaatgcgat tagccgatgg cggaacgaca aattttgtta atttttatga
      301 gttcgggcaa cacctttata gcaaactggc gtagagtctg atttcaggcc cgatataaag
      361 tggtaacgag gcgttccagc gcgggccagg ctcttacggt gcggcagaat ttccagtata
      421 atagccgcct tttttcattc agttgtgaca tacagctaac gctgcgactt ggtcacctgc
      481 gcagcagggg gcatccgccg gcagctaagt taagggatat ctcatgcgta cagaatattg
      541 tggacagctc cgtttgtccc acgtggggca gcaggtgact ctgtgtggtt gggtcaaccg
      601 tcgtcgtgat cttggtagcc tgatcttcat cgatatgcgc gaccgcgaag gtatcgtgca
      661 ggtatttttc gatccggatc gtgcggacgc gttaaagctg gcctctgaac tgcgtaatga
      721 gttctgcatt caggttacgg gcaccgtacg tgcgcgtgac gaaaaaaata ttaaccgcga
      781 tatggcgacc ggcgaaattg aagtgctggc gtcctcgctg actatcatca accgcgcaga
      841 tgttctgccg cttgactcta accacgtcaa caccgaagaa gcgcgtctga aataccgcta
      901 cctcgacctg cgtcgcccgg aaatggcgca gcgcctgaaa acccgcgcta aaatcaccag
      961 cctggtgcgc cgttttatgg atgaccacgg cttcctcgac atcgaaactc cgatgctgac
     1021 caaagccacg ccggaaggcg cgcgtgacta cctggtgcct tctcgtgtgc acaaaggtaa
     1081 attctacgca ctgccgcaat ccccacagtt gttcaaacag ctgctgatga tgtccggttt
     1141 tgaccgttac tatcagatcg ttaaatgctt ccgtgacgaa gacctgcgcg ctgatcgtca
     1201 gcctgaattt actcagatcg atgtggaaac ttctttcatg accgcgccgc aagtgcgtga
     1261 agtgatggaa gcgctggtgc gtcatttgtg gctggaagtg aagggcgtgg atctgggcga
     1321 tttcccggta atgacctttg cggaagcaga acgccgttat ggttctgata aaccggatct
     1381 gcgtaacccg atggaactga ccgacgttgc cgatctgctg aaatctgttg agtttgcagt
     1441 atttgcgggg ccggcgaacg atccgaaagg ccgtgttgcc gctctacgtg ttccgggtgg
     1501 cgcatcgctg acccgtaagc agatcgacga atacggtaac ttcgttaaaa tctacggcgc
     1561 gaaaggtctg gcttacatca aagttaacga acgcgcgaaa ggtctgggag gtatcaacag
     1621 cccggtagcg aagttcctta atgcagaaat catcgaagcc atcctggatc gtactgccgc
     1681 gcaagatggc gatatgattt tcttcggtgc cgacaacaag aaaattgttg ccgacgggat
     1741 gggcgcactg ctcctgaaag tgggtaaaga ccttggtctg accgacgaaa gcaaatgggc
     1801 accgctgtgg gttatcgact tcccgatgtt tgaagacgac ggtgaaggcg gcctgacggc
     1861 aatgcaccat ccgttcacct caccgaaaga catgactgct gcagaactga aagctgcacc
     1921 ggaaaatgcg gtggcgaacg cttacgatat ggtcatcaat ggttacgaag tgggcggtgg
     1981 ttcagtacgt atccataatg gtgatatgca gcagacggtg tttggtattc tgggtatcaa
     2041 cgaagaggaa cagcgcgaga aattcggctt cctgctcgac gccctgaaat acggtactcc
     2101 gccgcacgca ggtctggcat tcggtcttga ccgtctgacc atgctgctga ctggcaccga
     2161 caatatccgt gacgttatcg ccttcccgaa aaccacggcg gcagcgtgtc tgatgactga
     2221 agcaccgagc tttgctaacc cgactgcact ggctgagctg agcattcagg ttgtgaagaa
     2281 ggctgagaat aactgatatg actcaaatac acgaaatcat tcgcgttgca tcaaggcggc
     2341 aactgagtga actcccatga gcatagataa ctatgtgaat gggatgagcg aaggcagtca
     2401 acgaagaggc agcgtgaagg ataaagtgta taagcgtccc gtttcgatct tagtggtcat
     2461 ctatgcacaa gatacgaaac gggtgctgat gttgcagcgg cgtgacgatc ccgatttctg
     2521 gcagtcggta accggcagcg tggaagaggg tgaaaccgcg ccgcaagctg ccatgcgcga
     2581 agtaaaggaa gaggtcacca ttgatgttgt cgctgaacaa ctgaccttaa ttgactgtca
     2641 gcgcacggta gagtttgaaa ttttttcaca tttacgtcat cgctatgcgc cgggcgtgac
     2701 gcgtaatacg gaatcatggt tctgtcttgc gcttccgcac gagcggcaga tcgttttcac
     2761 tgaacatctg gcttacaagt ggcttgatgc gcctgctgcg gcggcgctca ctaagtcctg
     2821 gagcaaccgg caggcgattg aacagtttgt aattaacgct gcctgaacag gcagggctat
     2881 ctggagatat ttttatggca ggtcatagta aatgggccaa caccagacat cgtaaagctg
     2941 cgcaggatgc taagcgcggt aaaatcttca ctaaaatcat tcgtgagctg gtaaccgcgg
     3001 ctaagctggg cggtggcgat ccggacgcta acccgcgtct gcgtgcggcg gtggataaag
     3061 cactgtctaa caacatgacc cgtgacacac tgaaccgcgc aattgcacgt ggtgtgggcg
     3121 gtgatgatga tgcaaacatg gaaaccatca tctacgaagg ttacggtcct ggcggcacgg
     3181 caatcatgat tgaatgtctg tctgacaacc gcaaccgtac cgttgctgaa gtgcgtcacg
     3241 cattcagcaa atgtggcggt aacctcggca ctgacggttc cgtagcttat ctgttcagca
     3301 aaaaaggcgt gatctccttc gagaaaggcg acgaagacac catcatggaa gcagctctgg
     3361 aagcaggtgc tgaagacgtt gtgacatatg atgacggcgc gattgatgtc tacactgcat
     3421 gggaagaaat gggtaaagtg cgcgacgctc tggaagcggc aggtctgaaa gcagacagcg
     3481 cggaagtttc catgatcccg tccaccaaag ctgatatgga tgcagaaacc gcgccgaaac
     3541 tgatgcgtct gatcgatatg ctggaagatt gcgacgacgt gcaggaagtt taccataacg
     3601 gtgaaatctc tgatgaggtt gcagcgactc tctgatgatg cctgctaaac agcaaaacgg
     3661 agacgcgtga tggctattat tctcggcatt gacccgggtt cgcgcgtgac cggctacggc
     3721 gtcatccgcc aggtaggtag gcaactgtct tacctgggta gcggatgcat acgcaccaaa
     3781 gtggatgatt taccgtctcg tctgaagctc atctatgcgg gcgtgacgga aatcatcacc
     3841 cagttccagc ctgattattt cgccattgaa caagtcttta tggcgaagaa cgctgactca
     3901 gccctgaaac tgggccaggc gcgcggcgtg gcgattgttg cggcggtgaa tcaggaattg
     3961 ccggtatttg aatacgcggc acgtcaggta aagcaaacgg tggtaggtat gggtagtgcc
     4021 gaaaaaagcc aggtgcagca tatggtccgc accttgctga aactgcccgc taatccacag
     4081 gcggatgccg ccgatgcgct ggcgattgcc atcacccact gccacgttag tcagaatgcg
     4141 atgcagatga gcgaatcgcg gctgaaccta gcgagagggc gactgcgtta agttataccg
     4201 cctcggtcag ttctggctga ggcgtttcaa ctccctccgc gtgaatcaac accaaacccg
     4261 gatgatgcca caaactggct ggcgtgacgg ttttacgctc ccacggaata gaacctaaga
     4321 accagaattt ccagaaagtg agttttgcat tcggattgct atttaacagt tgtccaaacg
     4381 tctctatttc acccaccgga atacatagcg cctctttata aatatcaaag acaaattttg
     4441 a
//
LOCUS       DADMFL010000209         4408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.215, whole genome shotgun sequence.
ACCESSION   DADMFL010000209 DADMFL010000000
VERSION     DADMFL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.215"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..400)
                     /locus_tag="JNZ27_004032"
     CDS             complement(<1..400)
                     /locus_tag="JNZ27_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8666133.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIV"
     gene            complement(515..590)
                     /locus_tag="JNZ27_004033"
     tRNA            complement(515..590)
                     /locus_tag="JNZ27_004033"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(555..557),aa:Thr,seq:ggt)
     gene            complement(597..671)
                     /locus_tag="JNZ27_004034"
     tRNA            complement(597..671)
                     /locus_tag="JNZ27_004034"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(636..638),aa:Gly,seq:tcc)
     gene            complement(788..872)
                     /locus_tag="JNZ27_004035"
     tRNA            complement(788..872)
                     /locus_tag="JNZ27_004035"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(836..838),aa:Tyr,seq:gta)
     gene            complement(881..956)
                     /locus_tag="JNZ27_004036"
     tRNA            complement(881..956)
                     /locus_tag="JNZ27_004036"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Thr,seq:tgt)
     gene            1318..2268
                     /gene="coaA"
                     /locus_tag="JNZ27_004037"
                     /gene_synonym="panK"
     CDS             1318..2268
                     /gene="coaA"
                     /locus_tag="JNZ27_004037"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY8666134.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2297..3262)
                     /gene="birA"
                     /locus_tag="JNZ27_004038"
     CDS             complement(2297..3262)
                     /gene="birA"
                     /locus_tag="JNZ27_004038"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY8666135.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3259..4287)
                     /gene="murB"
                     /locus_tag="JNZ27_004039"
     CDS             complement(3259..4287)
                     /gene="murB"
                     /locus_tag="JNZ27_004039"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY8666136.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAMETIS"
BASE COUNT         1130 a         1102 c          997 g         1179 t
ORIGIN      
        1 acacgatgat gtacggaacg cctacctgac gccccagcag gatgtgctca cgagtctgcg
       61 gcatcgggcc gtcagtcgca gcaactacca ggatcgcgcc gtccatctga gcagcaccgg
      121 tgatcatgtt tttaacatag tcggcgtgcc ccgggcagtc tacgtgtgcg tagtgacggg
      181 tcggggtgtc gtattcaacg tgagaagtgt tgatggtgat accacgagct ttttcttccg
      241 gcgcgttatc gatctggtcg aatgcgcgag cagcaccgcc gtaggtttta gccagtacgg
      301 tagtgattgc agcggtcagc gttgttttac catggtcaac gtggccgata gtaccgacgt
      361 taacgtgcgg ttttgtacgt tcaaactttt ctttagacat cgattgtccc tctaagacac
      421 ggataaatcg gtgatatcac cacatcaacc aggcaacatg cccgacttgt tgaatgcaat
      481 aaacagaagg aaaaaacagg gaggagaaaa gaagtggtgc tgataggcag attcgaactg
      541 ccgacctcac ccttaccaag ggtacgctct accaactgag ctatatcagc acatcttgga
      601 gcgggcagcg ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa tagccattat
      661 acgatgcccg catcctagaa ctcggctacc tgattttcat tctgcactga atatcgagag
      721 aagctctctt tattcgagcc ggtaagtgaa cttatcgtct cgggctacgc catcgcgtgg
      781 ccgaaattgg tggtggggga aggattcgaa ccttcgaagt ctgtgacggc agatttacag
      841 tctgctccct ttggccgctc gggaacccca ccggacttga tggtgccgac taccggaatc
      901 gaactggtga cctactgatt acaagtcagt tgctctacct actgagctaa gtcggcatca
      961 agtagcgcgc attctatgga gacatgcgag ttcatgcaac taaaaaattg cataatttgt
     1021 tttattggtc acattttatg ctacacgatg aagaaacagc cgaacggagg gcagattagc
     1081 acactttttc aacatcattg tgctcaacaa tgcgctcctg ctaaaccata attcttttta
     1141 tcagatggaa tatctgtcac attgcttttc aacgatagct tcctggcaga gattttttct
     1201 tattattcct ccccatctgg tgttaccctc ctgcccatta acccattcaa cagaactgtg
     1261 acgcgccatg gcaaatatcg ctttgccgat agagctatga ccgccagaaa catgcttatg
     1321 agtataaaag agcaaacgtt aatgacgcct tacctacagt ttgaccgcaa ccagtgggca
     1381 gctctgcgtg attccgtacc tatgacgtta tcggaagatg agatcgcccg tctcaaaggt
     1441 attaatgaag atctctcgtt agaagaagtt gccgagatct atttaccttt gtcacgtttg
     1501 ctgaacttct atataagctc gaatctgcgc cgtcaggcag ttctggaaca gtttcttggt
     1561 accaacgggc aacgcattcc ttacattatc agtattgctg gcagtgtcgc ggtggggaaa
     1621 agtacaaccg cccgtgtatt gcaggcgcta ttaagccgtt ggccggaaca tcgtcgtgtt
     1681 gaactgatca ctacagatgg cttccttcac cctaatcagg ttctgaaaga acgtggtctg
     1741 atgaagaaga aaggcttccc ggaatcgtat gatatgcatc gcctggtgaa gtttgtttcc
     1801 gatctcaaat ccggcgtgcc aaacgttaca gcccctgttt actcgcatct tatttatgat
     1861 gtgatcccgg atggagataa aactgttgtt cagcctgata ttttaattct tgaagggtta
     1921 aatgtcttac agagcgggat ggattatcca cacgatccac atcatgtatt tgtttccgat
     1981 tttgtcgatt tttcgatata tgttgatgca ccggaagact tacttcagac gtggtatatc
     2041 aaccgttttc tgaaattccg cgaaggggct tttacagacc cggattccta ttttcataac
     2101 tacgcgaaat taactaaaga agaagcgatt aagactgcca tgacattgtg gaaagagatc
     2161 aactggctga acttaaagca aaatattcta cctactcgtg agcgcgccag tttaatcctg
     2221 acgaaaagtg ctaatcatgt ggtagaagag gtcagactac gcaaataatt tgcaggggag
     2281 cgaatactcc cctttcttat ttttctgcac tacgcaggga tatttcaccg cccatccagg
     2341 gttttattat tccatcctgc tcaagtaata aagccccctg tttgtctatt ccgcgtgaaa
     2401 tgccaaatat ttctttatca ccaatgataa gtttcactgg gcgattaata aaattatcca
     2461 gcttttccca gcgcgacaga taaggtgcca atccttcttg ttcgaagagt tccaacgcag
     2521 cacgtaattc acgtattagc atggccgcca acgtattacg atcaagattg atccccgctt
     2581 cctgcagcgt gatccacccc tgattaacga cactctcttc aacacggcgc attgccatgt
     2641 tgatcccgga tccaatgact atttgcgccg catcgccagt tttgccagtc agctccacaa
     2701 gaatgcctgc cagcttgcga tcctgcagat agaggtcatt aggccatttg acacgaactt
     2761 tatctgctcc cagctttcgt aatacttccg ccatcacgat accgataacc agacttaaac
     2821 caatcgccgc cgccgggcct tgttccagac gccagaacat cgacaaatat aagtttgcgc
     2881 caaaaggcga aaaccatttc cgcccccggc gaccacggcc agcctgctgg tattctgcaa
     2941 cacaggcatc gcccgattta agctctccga tacgatcaag aaggaactga ttcgtggagt
     3001 caataactgg cagcacggct acactaccgc catccagctg acccaatatc tgttcagcat
     3061 taagtaactg gatgggctca ggcaggctgt atcctttacc cggaacggta aagacatcaa
     3121 cgccccagtc acgcagtgtc tgaatgtgtt tattaatagc cgcccggctc attcccagcg
     3181 tttcacccaa ctgctcgcca gagtgaaatt caccgttcgc taacagggca atcaatttca
     3241 gtggcacggt gttatccttc atgaaattgt ctccattgcg ctcacttcac ctgatgcacc
     3301 aataaagcgg acttcaggct caagccagac attaaatttt tcacccactt tttgtcttac
     3361 atggtgtgcc agttgcacca catcttcgct ttttgcattg tcttcattaa tgagaactaa
     3421 cgcctgttga cggtgcaccg cagccccacc catttgcatc ccttttagct ggcactgatc
     3481 aataagccaa cctgctgcca gttttactga accacccgcc tgggggtaat ttggtgctgt
     3541 tggaaattgc gccagtaatg ctttagccgt ttcggcagat acaacagggt ttttgaagaa
     3601 actaccggca ttgccattca cttttggatc agggagtttg gtggtgcgca tatgacacac
     3661 cgcattaaat acttgctgtg gcgttactgt tgtaggatcc agacgagtta agtcaccata
     3721 cgttagtaca ggttgccact cttttggcag acgcagacct acggctacaa tggcgaagcg
     3781 atcctggtat tcatgtttaa aaatactgtc gcgatagcca aaacggcact ctttggcagt
     3841 taagcgcact tgcttgcctg tcgccagttc aacacaatca acataagcgc aaactcgctg
     3901 taattctacg ccataagcac caatattctg gataggtgat gagccgacac aaccaggaat
     3961 taatgccaga ttttccagac caggcatacc ttcctgcaaa gtgtatttta ccaggcgatg
     4021 ccagttttct ccggctccta catgtaaata ccacgcatca ggttcatcat gaatttcgat
     4081 acctttgatc cggttgatga tcaccgtgcc gcgatagtct tccagaaaaa gtacattact
     4141 tccttcaccc agaataagaa cgggttgtcc ttctgcggtt gcatgctgcc aggcattgag
     4201 taattgttgt tcgtcttcgg cacatacaat gtgctgagca ttatgatcaa tgccaaatgt
     4261 gttccagggt tttaaggagt ggttcatagc tgctttcctg atgcaaaaac gaggctagtt
     4321 taccgtatct gtggggggat ggcttgtaga tatgacgaca ggaagagttt gtagaaacgc
     4381 aaaaaggcca tcctgacgga tagccttt
//
LOCUS       DADMFL010000210         4403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.216, whole genome shotgun sequence.
ACCESSION   DADMFL010000210 DADMFL010000000
VERSION     DADMFL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.216"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            77..1222
                     /locus_tag="JNZ27_004040"
     CDS             77..1222
                     /locus_tag="JNZ27_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8666137.1"
                     /translation="MGVILMSLNMASLETLCQTNAAGVSIVISSLGIGRLSVLLFAGL
                     LSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLDAGTYPSLME
                     AFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINALFLYR
                     CTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQWLAQYGQFV
                     AGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHP
                     TFYVVIIFAFVIGFTSAGGVVQIGLTLIAERFPYAKGKATGIYYSAGSIATFTIPLIT
                     AHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            1462..2676
                     /locus_tag="JNZ27_004041"
     CDS             1462..2676
                     /locus_tag="JNZ27_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8666138.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPHLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            2688..3554
                     /gene="ydiB"
                     /locus_tag="JNZ27_004042"
     CDS             2688..3554
                     /gene="ydiB"
                     /locus_tag="JNZ27_004042"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY8666139.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            3585..4343
                     /gene="aroD"
                     /locus_tag="JNZ27_004043"
     CDS             3585..4343
                     /gene="aroD"
                     /locus_tag="JNZ27_004043"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY8666140.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
BASE COUNT         1085 a          994 c         1034 g         1290 t
ORIGIN      
        1 tttgctaagt agaacctatg aaaaatccct attaccctac cgcacttggg ttgtatttta
       61 ttacctggtg catggtatgg gcgtcatttt gatgagcctg aatatggcct cgctggagac
      121 actttgtcag actaatgccg cgggtgtctc gatagttatc tcatcgctgg gcattggtcg
      181 attaagtgtc ttgctttttg caggattatt atccgatcgc tttggtcgcc gcccttttat
      241 catgctcgga atgtgctgct atatggcctt cttttttggc atcctgcaga ccaataacat
      301 cattatcgct tatgtttttg gctttctggc gggaatggca aacagttttc tcgatgcagg
      361 cacttatccc agtttgatgg aagcttttcc acgctcacct gggacagcca atattttaat
      421 taaagcattt gtttccagcg gacaattttt attaccgcta atcattagcc tgttagtgtg
      481 ggctgaactg tggttcggtt ggtcctttat gattgctgca ggcattatgt ttattaacgc
      541 tctgttttta taccgttgta cgttcccacc ccatccgggt cgtcgcttac ctgtcataaa
      601 gaaaaccacc agctctacgg aacatcgctg ttcaattatc gatttagcca gttatacctt
      661 atatggctat atctcaatgg caacgtttta tctggttagc cagtggctgg cacagtacgg
      721 acaatttgtt gcaggcatgt catacactat gtcgatcaaa ctactcagta tctacaccgt
      781 gggttcgctg ctttgtgtat ttattaccgc tccactcatt cgtaataccg ttcgcccaac
      841 aacattactg atgctgtaca cctttatctc atttattgct ctgtttaccg tctgcctgca
      901 tcccacattt tatgtggtga taatatttgc ttttgtcatt ggttttacct ctgctggagg
      961 tgttgtgcaa attggcctga cgttaatagc tgaacgtttc ccttacgcta aaggtaaagc
     1021 tacagggatc tattacagtg cgggcagtat tgcgaccttt actattccgt tgattacggc
     1081 tcatctgtcc caaagaagta ttgccgatat tatgtggttc gatactgcca tcggtttttt
     1141 actggcactg tttatcggct tacgcagccg caaaaaaacg cggcatcact cgctaaagga
     1201 aaatgtcgct ccgggtgggt aatgcaatat tcttttcagg tcatgcaaga tcttacggat
     1261 aaataactct ttctgcgcta actaaggaaa atcgcgatca aaaacaaact atgacatgca
     1321 atattcctgg aaacataaac tttatgccat gtacccaggg aaaatcatct tcagtatagt
     1381 aattatgtaa accgtcggag aacaatacgt acggtaacga aattatcttt cagcacgcca
     1441 tttatcctgg ctgttctttg tatttacttc agctatttcc tgcacggcat tagtgttatt
     1501 acgcttgccc aaaatatgac atctctggca gaaaagtttt ccactgacaa cgcgggcatt
     1561 gcctatttaa tttccggtat cggtttgggg cgattgatca gtattttatt cttcggtgtg
     1621 atctccgata agtttggtcg tcgggcggtg atattaatgg cagtaataat gtatctgcta
     1681 ttcttctttg gtattcccgc ttgcccgcat ttaactctcg cctacggtct ggcagtgtgc
     1741 gtaggtatcg ctaactcagc gctggatacg ggtggctacc ccgcgctcat ggaatgcttt
     1801 ccgaaagcct ctggttcggc ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg
     1861 ttctacccaa tgctggtgag ctatatgttg ctcaataata tctggtacgg ctatgggctg
     1921 attattccgg gtattctgtt tgtactgatc acgctgatgc tgttgaaaag caaattcccc
     1981 agccagttgg tggacgccag cgtagctaat gaattaccgc aaatgaacag caaaccgtta
     2041 gtctggctgg aaggtgtttc atcggtactg ttcggtgtag ccgcattctc gaccttttat
     2101 gtgattgtgg tgtggatgcc caaatatgcg atggcttttg ctggtatgtc agaagctgag
     2161 gcattaaaaa ccatctctta ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc
     2221 gcactactga aaaaaatggt ccggcccatc tgggctaatg tatttaattc tgcgctggca
     2281 acaataacag ctgccattat ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc
     2341 tttgttatcg gtttctcagc agctggcggc attttacagc tcggtgtttc ggtcatgtca
     2401 gagtttttcc ccaaaagcaa agccaaagtc accagtattt atatgatgat gggtggactg
     2461 gctaactttg ttattccgct gattaccggt tatctgtcga acatcggcct gcaatatatc
     2521 attgttctcg attttacttt cgcgctgctg gccctgatta ccgcaattat tgtttttagc
     2581 cgctattacc gcgtttttat tattcctgag aatgatgtgc ggtttggcga acgcaaattt
     2641 agcacccggt taaacacaat taagcataga ggttaaagga gttaattatg gatgttaccg
     2701 caaaatacga attgattgga ttgatggctt atcctatccg ccacagttta tcgcctgaaa
     2761 tgcagaataa agccttagaa aaagcgggat tgccatttac ctatatggcc ttcgaagtgg
     2821 ataacgatag ctttcccgga gcaattgaag gattaaaagc cctcaaaatg cgcggaactg
     2881 gtgtgtcgat gccgaacaaa caactggcgt gtgaatatgt tgatgaatta acgccagcag
     2941 ccaaactggt gggtgccatc aacaccatcg ttaatgatga tggctatctg cgtggctata
     3001 acaccgacgg cacgggccat attcgcgcca ttaaagagag cggttttgat atcaaaggca
     3061 aaacgatggt gctgttaggg gccggaggtg cctcaacggc gattggcgcg cagggggcaa
     3121 ttgaaggttt aaaagaaatt aaactcttta accgtcggga tgagttcttc gataaagccc
     3181 tcgccttcgc gcagcgggtt aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg
     3241 ccgatcagca agcctttgct gaagccctgg cttccgccga cattttaacc aatggcacaa
     3301 aagtaggtat gaaacccctt gagaatgaat cattggttaa tgatatcagt ctgttacatc
     3361 cgggactgct ggtcactgaa tgcgtgtata acccgcatat gacgaagtta ttgcagcagg
     3421 cacaacaagc tggttgcaaa acgattgatg gatacggcat gttgttgtgg caaggggctg
     3481 aacagttcac gttatggact ggcaaagatt tccctctgga atatgttaaa caggtcatgg
     3541 ggttcggtgc ctgacaggct gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg
     3601 taaaagatct cgtcattggt gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag
     3661 atatcgcccg cgtgaaatcc gaagctctcg cctatcgtga agcggacttt gatattctgg
     3721 aatggcgtgt ggaccacttt gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa
     3781 aaattctccg tgaaaccatg ccagaaaaac cgctgctgtt taccttccgc agtgccaaag
     3841 aaggcggcga gcaggcgatt tccaccgagg cttatattgc tctcaatcgt gcagccatcg
     3901 acagcggcct ggttgatatg atcgatctgg agttatttac cggcgatgat caggtcaaag
     3961 aaaccgtcgc ctacgcccac gcgcatgatg tgaaagttgt catgtccaac catgacttcc
     4021 ataaaacgcc ggaagccgaa gaaatcattg cccgtctgcg caaaatgcag tccttcgacg
     4081 ccgatattcc taagattgcg ctgatgccgc aaagtaccag cgatgtgctg acgttgcttg
     4141 ccgcgaccct ggagatgcaa gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg
     4201 caaaaactgg cgtaatttct cgtctggcag gtgaagtatt tggctcggcg gcaacttttg
     4261 gtgcggtaaa aaaagcctct gcgccagggc aaatctcggt aaatgatttg cgcacggtat
     4321 taactatttt acatcaggca taagcaataa tatttcggcg gaaataccct cccccgccga
     4381 aataaaaaaa gtatttttaa ccg
//
LOCUS       DADMFL010000211         4402 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.217, whole genome shotgun sequence.
ACCESSION   DADMFL010000211 DADMFL010000000
VERSION     DADMFL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.217"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..157)
                     /locus_tag="JNZ27_004044"
     CDS             complement(<1..157)
                     /locus_tag="JNZ27_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666141.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNZ27_004045"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNZ27_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY8666142.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JNZ27_004046"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JNZ27_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8666143.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDTTKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNZ27_004047"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNZ27_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8666144.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..4333)
                     /locus_tag="JNZ27_004048"
                     /pseudo
     CDS             complement(3800..4333)
                     /locus_tag="JNZ27_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
BASE COUNT         1169 a         1097 c         1013 g         1123 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg tttttgtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacgacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatacca cgaaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgcggac tgaggtagcc tgagtttaac ggacactcct tcctgaaata gaatggcatc
     4381 agaaggagct aataatgagc ag
//
LOCUS       DADMFL010000212         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.218, whole genome shotgun sequence.
ACCESSION   DADMFL010000212 DADMFL010000000
VERSION     DADMFL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.218"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(64..822)
                     /locus_tag="JNZ27_004049"
     CDS             complement(64..822)
                     /locus_tag="JNZ27_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY8666145.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JNZ27_004050"
     CDS             complement(830..1933)
                     /locus_tag="JNZ27_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY8666146.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JNZ27_004051"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JNZ27_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JNZ27_004052"
     CDS             complement(3181..4206)
                     /locus_tag="JNZ27_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8666147.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYDLRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1216 a         1146 c         1020 g          996 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatactg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg catggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaagtcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADMFL010000213         4339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.219, whole genome shotgun sequence.
ACCESSION   DADMFL010000213 DADMFL010000000
VERSION     DADMFL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.219"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..324
                     /locus_tag="JNZ27_004053"
     CDS             <1..324
                     /locus_tag="JNZ27_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY8666148.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JNZ27_004054"
     CDS             383..586
                     /locus_tag="JNZ27_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY8666149.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            586..1611
                     /locus_tag="JNZ27_004055"
     CDS             586..1611
                     /locus_tag="JNZ27_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8666150.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="JNZ27_004056"
     tRNA            complement(1664..1753)
                     /locus_tag="JNZ27_004056"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="JNZ27_004057"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="JNZ27_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY8666151.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="JNZ27_004058"
     tRNA            2745..2820
                     /locus_tag="JNZ27_004058"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..4244
                     /locus_tag="JNZ27_004059"
     CDS             2982..4244
                     /locus_tag="JNZ27_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8666152.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1212 a          998 c         1036 g         1093 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa
      541 aactttacca ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatatccctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggcagt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact aacgaatccc
     4261 acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac ttttatcaac
     4321 tttatgacga aaaacacct
//
LOCUS       DADMFL010000214         4213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.222, whole genome shotgun sequence.
ACCESSION   DADMFL010000214 DADMFL010000000
VERSION     DADMFL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.222"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..439
                     /gene="tnpA"
                     /locus_tag="JNZ27_004060"
                     /pseudo
     CDS             1..439
                     /gene="tnpA"
                     /locus_tag="JNZ27_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JNZ27_004061"
                     /gene_synonym="qseD"
     CDS             complement(557..1501)
                     /gene="hypT"
                     /locus_tag="JNZ27_004061"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY8666153.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1846..2565
                     /locus_tag="JNZ27_004062"
     CDS             1846..2565
                     /locus_tag="JNZ27_004062"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8666154.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2631..3929
                     /locus_tag="JNZ27_004063"
     CDS             2631..3929
                     /locus_tag="JNZ27_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8666155.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          914 c         1037 g         1202 t
ORIGIN      
        1 gcgcacaccc gatggaactg taaatatcac atagtttttt gcgccaaaat accgaagaca
       61 ggtgttctac agagagaagc gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg
      121 gaaaagcgta cggattctgg aagctgaatg ctgtgcagat catatccata tgcttgtgga
      181 gatcccgccc aaaatgagcg tatccggctt tatgggatat ctgaaaggga aaagcagtct
      241 gatgctttac gagcagtttg gtgatttgaa attcaaatac aggaacaggg agttctggtg
      301 caggggggac tacgtcgata cagtgggtaa gaacacggcg aagatacagg attacataaa
      361 gcaccagctt gaagaggata aaatgggaga gcagttatcg attccctgtc cgggcagccc
      421 gtttacggac cgtaagtaac gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg
      481 gcgcggctgg taagagagcc ttataggcgc atttgaaaaa cctccggcta tgccggagga
      541 tatttattgc acgtcgtcaa ttgcggcgtg ctggctcttc cattgatatc tgctggatta
      601 aatcgtctgg catatgttcc tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc
      661 gggtgttcat tcggtaaata taagcctgaa tggggactgt caatttagca gcatcaaggc
      721 atatcagacg tttggtctgt agttcatcga cgatcgacca gatcggtaac caggcaatgc
      781 cgtatccgtt gagcgccata ttctttaata actcgctcat cgaagagata aatatcgtgc
      841 gcccggtaat acctccaacc tctgccagat gacgattgac caacctgccc atataagatg
      901 tggcggtata gttgagcaaa ggaacctggg gctgagtgat atcgaataca gggtgcccct
      961 gtgcgtcggc ggcacatacc gggtacaact cagattcaaa cagtttcagg cagcaaaaag
     1021 gcgattgcat aagatcttca tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta
     1081 acgtatttac ggcctggaca acatcaatgg cttcaacatg atagacaaat tctccgccat
     1141 aggcggtaag tgaatgcacc agtttgggta atacgctcag ggatagtgag tgcgcggctg
     1201 caattttaat gttgggaacg ccgagtaaac tctggccgtt taactccccc aaattgcatt
     1261 caagctgctg taataagctg cgagtctggg aatgaaagag cttgccctct tcggttaatt
     1321 gtaaaggggt ggtcgtgcga tcgaataaaa caacgccgat cgctgcctcc agcgccttaa
     1381 ttcgtcggct gaatgcgggt tgcgaaagat tacgctcttt tgccgcctga gaaaaatggc
     1441 gacaggcttc cagtgtcagg aaatcgtata accattttgt ctcgatattc ttcattacca
     1501 tggcacctgt attttcaatc caaagacata ataaaacatc ccggcaataa aaataatgtt
     1561 ctcaattagc acaacctggc ctttatcatt cgttttgtgc ataacccctt catttccggt
     1621 attgacgttg atatcacatt tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat
     1681 acatcatcac tatggtgaat aagcggaatg ttccatagct gcatcatgcg tgtcttgcat
     1741 cgccttgtac aaccttgcat ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca
     1801 gttatttacc gcgtattgtc attaacgttt tgagaggcag gtattgtgaa aggtttaatt
     1861 ggtgtactcg gtggaatggg gccggcagct accgtcgacc tgtttaataa atttgtaact
     1921 tttaccgctg cacaatgtga tcaggaacat attcctttaa ttatttcttc gatcccggat
     1981 attcctgacc gtaccgatgc attaatgcat catgggcgtt caccattacc cgttatgcgt
     2041 gattatatgc ataagctgga agatgccggg gcagaatgta ttgttattcc ctgtaatacc
     2101 gcgcattact ggttcaaaga attaaaagat gcttgtcata ttgatatatt aagtatcgta
     2161 gaaactacga tcaatgaagt tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc
     2221 aatgccacgc tatatatggg tttataccaa aaaggaattg agtcgctggg atttacctgt
     2281 gtcagccccg atgccgacgg tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct
     2341 ggcaatatag cgcacgcaca aaagttgatg aatgagcagg ctgaaatatt attttcccgc
     2401 ggtgcagaaa tccttgttct tggttgtaca gaaattccgg ttattctggc gcaagccgtt
     2461 aaagagcagc cttcccgcta tattgactca acggcgtcac tcgttcgtgc cggaataaaa
     2521 tggtacgaaa atcgtgtcgg taaacattat cttttaaccc aataatcatt taaatcgcag
     2581 ccagaaagtt attcgctttt aactgaatta tatttataac ggagaacatt atggtttggc
     2641 tggaaattat cgtagtactt ggtgcaatat tttttggtat tcgccagggg ggaatcggta
     2701 ttggtttatg tggcgggctt gggcttgcca ttctgactct gggatttggt ctgcctatgg
     2761 ggtcgccgcc agtcgacgtg atcctgatta ttatgaccgt tgttgtggcg gcctcagcct
     2821 tacaggccgc aggcggaatg gattatctgg tacgcgtcgc tggtaatttt atgcggcgta
     2881 acccgaaata tatcaacata atcgcgccga ttatcacctg gtctatgacc attatggccg
     2941 gtactgggtt tattgttttt tccacgctgc cggtgattgc tgaagtggcg aaagagtccg
     3001 gtatccgtcc ttcccgcacg cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg
     3061 gttcgccgat cagcgccgca atggcggcta tgctcactat tatggaggta aacggtgtca
     3121 gttttattca agtgatgtcg gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg
     3181 cttttattgc ttcccgacag ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc
     3241 tgcaaaaagg actggtgcaa aaatacgaga ataatagcag catcaagcca ggtgcgacgc
     3301 tctccgtcgg attgttcctg ctggcgacgg tcgctattgt tattcttgct gccttccccc
     3361 aactgcgtcc gggttttgat atcggtaaac cgatggaaac acgcgatatc attattatct
     3421 gtatgctctc tgccgcctgc ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta
     3481 ttctcacctc cacattccgt gcggggatga gttcgctggc ggtaattctc ggcattgtaa
     3541 ccttaggcac cacttttatt gatgcgcatt taactgagat taaagatatt gctggtgata
     3601 ttttgcaggc gtatccgatg ttgttggcgc tggttctttt tggcacttgc gcgctgctct
     3661 actcgcaagg tgcgaccacg ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca
     3721 cctgggcaat tctggcgtcc tatgtggccg tcaccggagt atttgtactg ccaacgtatc
     3781 caacatcact ggctgccatg gaatttgata ctaccggcac aactcgtgta ggcaaatatg
     3841 tgctgaatca tccatttatg ctgcctgggt tggggggaat tattgctgga gtggcattag
     3901 gctttgtgat tgcgccaatg attatttagc actatcaaac atcagtatcg atggtttata
     3961 cacaataaag acagatatgg cggaatatag tctgttgtaa gcgccaacaa agaatagggg
     4021 aattccgcca tgtctgaaca tcttcacaat atggtattaa gtgttgtcgt ttaatgattt
     4081 atttcttggt tatgttgtgt attagctgca tctatcgctt tttgatacct gaggtgtact
     4141 ggcaatagcg gacactacca tttgttcttt ttttaagcag tcatctgatg atatttttcc
     4201 ctgaaggctg ccg
//
LOCUS       DADMFL010000215         4190 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.223, whole genome shotgun sequence.
ACCESSION   DADMFL010000215 DADMFL010000000
VERSION     DADMFL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.223"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(8..355)
                     /locus_tag="JNZ27_004064"
     CDS             complement(8..355)
                     /locus_tag="JNZ27_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8666156.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(446..790)
                     /locus_tag="JNZ27_004065"
     CDS             complement(446..790)
                     /locus_tag="JNZ27_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666157.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVDLLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(817..984)
                     /locus_tag="JNZ27_004066"
     CDS             complement(817..984)
                     /locus_tag="JNZ27_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666158.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(1172..2011)
                     /locus_tag="JNZ27_004067"
     CDS             complement(1172..2011)
                     /locus_tag="JNZ27_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8666159.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRDHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(2013..2699)
                     /locus_tag="JNZ27_004068"
     CDS             complement(2013..2699)
                     /locus_tag="JNZ27_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8666160.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(2686..3000)
                     /locus_tag="JNZ27_004069"
     CDS             complement(2686..3000)
                     /locus_tag="JNZ27_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666161.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2997..3482)
                     /locus_tag="JNZ27_004070"
     CDS             complement(2997..3482)
                     /locus_tag="JNZ27_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666162.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(3479..3643)
                     /locus_tag="JNZ27_004071"
     CDS             complement(3479..3643)
                     /locus_tag="JNZ27_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY8666163.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(3654..3947)
                     /locus_tag="JNZ27_004072"
     CDS             complement(3654..3947)
                     /locus_tag="JNZ27_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY8666164.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3961..>4190)
                     /locus_tag="JNZ27_004073"
     CDS             complement(3961..>4190)
                     /locus_tag="JNZ27_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY8666165.1"
                     /translation="GQDRYTTEVHVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSP
                     AQHNEPPMDFDDDIPFAPVTLPFPRHAIHAI"
BASE COUNT          919 a         1070 c          928 g         1273 t
ORIGIN      
        1 tctgctttca gacgatcgtg aagttcttgc gcttgccttt tgtccgatgt ttcaagagag
       61 cgtctaactc ttgatccatc tggcgcgacg aaatcgcagt gccacgtgcc accgcgtagt
      121 ttgattgaca tgctttaacc tcctgcacat caaccgcatt caccgcgcta ttgtgtctca
      181 cagacttaag cgccgcaatg cagtctgact tgcaaatgcg atatgggctt ttaggtttat
      241 ctggatttat ctttacggcc tgaagtcgtc cacttcgtat ccactgcgtg atagtgcctt
      301 tgtctacctt cagatacgac gctgcctctt cacgagtgaa gatttcttct tccacctgga
      361 atctccattt attggattgg tattattgcg gtaggtctgg atatcattga gcaatgaaca
      421 ggcctcatcg agtatgaggc tgtggttagt ccttgcgtaa ctcgctaatt cttctgtaag
      481 tctctggtgc tttgtttccg tgtatcttca tttcagactt caacagatca acgagggaat
      541 cccattcgtt gaggatgcct ttgaatgccg gaacgcgctt tgcaaccttg tcgaatgaat
      601 ctctgatttc tgggatctgc tcaacaagtg caacgcatcg tctgaaatcg gctgcgtcat
      661 ggggagcgcc gaagtgatga ccatagatat tctttttcag tccacatgcg attgaggcaa
      721 gagttgcgct actgatgcca acatcgccag tcgattgcca tttcaaaacc ttcatagcca
      781 aatctgacat ttcttgtctc caataaaaaa ccgccatcag gcggcatggt gttctttcag
      841 ttcctcaatt cgaatattgg ttacgtctgc atgtgctatc tgcgcccata tcatccagtg
      901 gttatagcag tcgttgatgt cctctgcttc gataaccctg tcgaatggct ctccattcca
      961 ttcacctgtg actcggaagt gcatttatca tctccataaa acaaaacccg ccgtagcgag
     1021 ttcagataaa agaaatcccc gcgagtgcga ggattgttag ttgctatctg ctgcttcctt
     1081 ggtcattacc atatccaccc aatttcacca gacatgattc tcgcaatcac tattatcacc
     1141 aaagtgataa tcacaacttt aactggcggc atcattcacc atcctgctcc ggcggttcta
     1201 gcagcggcat ccagtgggtt acgtcatcca agatatttcc tgataaatat gtgaaagctc
     1261 tatatttttt gtaatcaatt ggactcatta cccagttcca atatgctgcc actatttgac
     1321 cttgactaaa tgccagtaac attttggtgt cttccggcat tcgctcacta cagcttatcc
     1381 aaccatccgg agttaccgga tagttgcgca ttgcgacctt taatgcctca tagaagcaac
     1441 ctttcagatt gttgaactga cgcccattta gcgggccatg ttcagtcagc atgttgtgta
     1501 atttccatgc cgcgtcgttt acttcgttgg atgacagggg aggcaacttg taagtttggc
     1561 ttacaggttc ggcaccatga agcatggcgt cgctccgctc tatgccatcc agcgcgattc
     1621 gtagtgcctg aattgtggta gagctatcgt ttggggctat tccatatcgc tcgaatacag
     1681 ctaaatggtc gcgcataatc tcaggcgtaa gctctttgta agcataagcg agaggctctg
     1741 atgcattatc cggcacaacc gacgcaggcg cggcagcata aacaggaata acgtccgctt
     1801 gctctttatt gctttcatcc gttaaagccc agaataattt cccggccgga tgtttgaaaa
     1861 tataagcaac tggctctgct tccagcgatg ccagtgcaat tcgtgccagt tcttccgctt
     1921 cttctgctgg cagtacaacg ttgctaccag gtccgtatgt ttcgcgccac tgcttgattg
     1981 tcagtagtcg ctctttggta atagtggtca tctcactctc ctttgatgcg aataccagta
     2041 gcgcggattg catcgatgac ttcagaaact ttgtatgcca ttaccgtttg gtaatcctcg
     2101 tgaaaatctg ttcgatgaag catgctgcta cgttccggga gcagtatttc ccgctcctcc
     2161 agttctgcta tgcgcttctc tgcggattcc agcttctcgc gcatatcgtc aacgtactcg
     2221 accagagaac cgccagcagg aatttcgcac tcctcgacca gttggaagta gatatcagct
     2281 gcggcccgtg tgttgctata cctagcgtcg cccatctcac cttcacgaag agcatcgcgt
     2341 tcggcggtaa gattggctat tttgctgtct ttgccttcca gctcaacgcg cagcttccct
     2401 accgtaagcg caatatcctc gttctcctgg tcgcggcgtt tgatgtattg ctggttcctt
     2461 tcccgttcat ccagtagtgc ctgcgctact tcagggttga aagctgcgat ataacgagcg
     2521 ttgttctctg cgttgttctg tccatcaaag ccggtccatt tgataacgtc ttcacatcgt
     2581 ttatcaccag gtgtatgcac cgcatatgta ccagtaccag acgaaataaa tgcgacccat
     2641 tcaccctgtg ttgccagttg cgccgcttta cgcagtgcct ggtaattaat ttcgctcact
     2701 ggttgcctcc tttgctgggc tttctaactt ttgagtggtt gtatcaaatt caaacaactt
     2761 aaccacgtca tcaaacagga cataatcacc atcagaatct tcagtcatgt cagcgccaca
     2821 atcctgaccg catgagtcac aaccacccat atcaagctcg tatcgcttca ggtttgcgat
     2881 atttgataaa ttcagcgcca gtacagccag gtcataaacc tcttcggcag tgacatcgct
     2941 gctcagtccc atttcatggc gatatatgat tttttctact cgttgttttg tgattgtcat
     3001 ttttgctcgc ctccctgttc ttccagaaaa atacgcatag tctcaagcat ctcttcggtg
     3061 tcatacggtg acaacttgtc acgcaggatg tgttcaatgc tgttaatgaa cttgcggatt
     3121 gctttgcgtt caatttcagc cagaaaagca tcggttgccg gggtttcagg tttaatggct
     3181 tcaagcacgg cgcgaatgac atcggtatcg ttctccaccc atgaccattc gcttgtatca
     3241 ttccagtcgt ggtctatgat agctgtttca ttgaatgcct cgactgccgt atccgggatt
     3301 acttcatgat ttaaagctgc cttcagcccc gcattctccg ccgccagcgc cgcgcacttg
     3361 gcctccgctt cagcaaattt acgcaccaga tattcagcgt ttgtttcgtt aacctttaaa
     3421 tcacttggga tgcatttacc tttcagaaat ccatccatct caattagtgt catttgtttc
     3481 atttcttccc attccgccac attgcattca gatatttgtt ttgatttact gacggaaaag
     3541 aatttctttt aagcaattcc tctctcgatg gcattggctt tacgcgttgg cgaataatca
     3601 tttctgccgg aagaatgccg agattgtatg caagtcctct catggtaaat tcctcagtca
     3661 ttactgatag cgccatagcg tgagcggtaa ttacgcaggc gcgggtcaat ttcagggaag
     3721 tgggtatatg tggctttgcg gaatggtcgg attgatgtct ggtaaattcg ctcgcgctct
     3781 tctttctctg caagccatat gcagtggcga aattcctttt cctctttcgt ttcctgcggt
     3841 agcgacatta tccggtcgta gtttttcctg aatttatcca gcacctccga tacggaattg
     3901 ccggaacagc ggcgcgggtc atccgcacca tactgaggcg ctggcatgat tttctcctga
     3961 ttaaattgcg tgaatagcgt gacgagggaa ggggagagtt actggtgcaa agggtatatc
     4021 gtcgtcaaaa tccatcggag gttcgttgtg ttgtgctggt gatgattgct gctgtggctt
     4081 ctgtgattgc ctgctggctg cttgttgttt gctgtcgcca atgccgccaa gcatttgcat
     4141 cacgccatta attccgacat gaacctcggt tgtgtaacgg tcttgccctg
//
LOCUS       DADMFL010000216         4115 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.224, whole genome shotgun sequence.
ACCESSION   DADMFL010000216 DADMFL010000000
VERSION     DADMFL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.224"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            246..1201
                     /locus_tag="JNZ27_004074"
                     /pseudo
     CDS             246..1201
                     /locus_tag="JNZ27_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1198..2103
                     /locus_tag="JNZ27_004075"
     CDS             1198..2103
                     /locus_tag="JNZ27_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY8666166.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2100..3170
                     /locus_tag="JNZ27_004076"
     CDS             2100..3170
                     /locus_tag="JNZ27_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY8666167.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3306..3983
                     /locus_tag="JNZ27_004077"
     CDS             3306..3983
                     /locus_tag="JNZ27_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666168.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCCWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1070 a          969 c         1140 g          936 t
ORIGIN      
        1 gctcacttat cgatcaactg ataacggaag tcatactgag ttcacactcc agagctgcgg
       61 cactggcggt tgattctgcc gccgcaaaac tgatgcagaa tgcagacaat atcagtgaat
      121 acctgtcgtt acaccatcag gggctgatgc aaagtattca gtcactgcag gcgcatatca
      181 ccagcctgct tgaggatatc cgggaaatag cagactgtca ggagcaggtg accgctgatg
      241 tcagaatggc catggaggag atcgatgcca gaacccggga attactgacg gggtctgcac
      301 ctcactggaa gaggagctga atgactattt cagaagcggt aaacgcaaag aacagcaaat
      361 gctggaggaa gaaaattcag cgcagccccg ggagagaaac gcattcgctt tctttcacga
      421 tattttcgga accggaaatc agcatgacag gatgcgggac tttgatccgg acagccccga
      481 aataaaattc agtgatcgca gggaagccct tgaactgatg acgcaaatcg aatcgaccgt
      541 gaccagcctg caccgagagg ctgaagcaca gttccggcct gagctggaga aaatcgtcag
      601 cgggattgaa acaggttttc gtggcacggc cctgtacgcc acagaaaaca ttgccggtcg
      661 catcaatacc cgcctggagg atgagggctt taccgtaaaa atcagttttc cggcagtcag
      721 tcagttacaa acccggctcg cggtaaaaac aaatctgagt gcgcttatgg aggaaagaac
      781 ggagaccgtc acccgtcgcc gtcggcagcg tggtttatgg ggaaagattt gtggagcgtt
      841 tggcaccagt gactggggct gggaaaccta taaagaggat gtgagccgca gtgttatcaa
      901 tatcaacacc gtcaggaagg aagttatgtc actgacccgg gcatatttcg gggagctgca
      961 ggcatccatt gagcaggata ttaaccagcc cgtccgccag gaaatcgatg cctttttctg
     1021 cgcattcagg gagaaagttg aacaactgcg taacacgctg attcagagct ctgaagatca
     1081 taaacgcgat cagcaggcac aggaacggct taccaggcga cttcaggcat taaatgaaag
     1141 ggttcctgag ctcattactg acagtaaggc gctgagggaa gagctggaga caatgctgtg
     1201 acttcaccat ttattcaaca aattgctgat aaccgggtat gtcaggtact cacctgtctt
     1261 cctgaaaaat ttgtggttga tttcgcaaac ggaatcgatg ttgcacagga gcatatccgc
     1321 acggccggag agcgcacgtt cttccggcgt ttaaaagagg gactgaccgg cgaaggcgct
     1381 gcacggcaga atgccatcaa tgcttcggtt gcacagggcg ttgaggcgtc cctgcgctgg
     1441 ctgacggagc tgacaacgtc acttgccacc acaaactacg cgattacccg ggtaaacgac
     1501 agggtcagtt cactggtcag tgatactgcc aggttggcgc attattccgc agatacacgg
     1561 gagcagttac tcatcctggc cgatcaggtt caccataaac tgaatcatct tgaagaaaaa
     1621 ctccaccgtg ttgaccaggt tcagcgggcg cagttacatc ttgagcagat attctcatgg
     1681 tggagcgccg ggcgatacgc gtcattttcc cctgccggac gttgttatgt ggcacttgaa
     1741 gagcttcgct ggggcgcgtt tggtgatgtg atacgtcagg gcgaaacagg ccaggttaac
     1801 cagctactgg atattctcag acataaagca ttaacgcaga tggcacagga gagtggcggt
     1861 agtgcaaccg tgcgtctgaa cactctggac tggcttggtg gtcagggccg ggagcaggca
     1921 gataacgagt ggcatgacgc gattaactgg ctgggagact ggtgcagtga agagcagcat
     1981 ccggtgatct ggtcaaccac gcaggctgca gaacacttac cggttcgcat gcctcgcctc
     2041 tgctctgcag aacgcctctc tgaaagtatg gtcgatgaaa tatttcagaa gggggctgca
     2101 tgagtacaga aatgaaaacg gggctggtgc tgtccggtgg cggggcggtg ggcgcttatc
     2161 aggcgggagt ggttaaggca ctggcagagt gtgatacaca gatcagcatg gtttcagggg
     2221 ccagcattgg cgcattcaat ggtgccatta tcgcggcctc tcccgatctg tcagaagctg
     2281 ccgtacgcct ggaagcgctc tgggatcatc tggggaataa tcaggtgctg tcggtaaacc
     2341 gattggttta cttttcattg ctgaaaaaat tgttccaggc aatgaacctc tgccagatcc
     2401 ccggacgtgc aggagcactg cttacgacgc ttcttcgcca tatatcgata ctcaacgggt
     2461 ttgacaatct gatggctcag ccgttgttgt cagatgagcc cctgacagcg ctgttggatc
     2521 attatcttga tactgatgct ctggcagacg ggctaccgct gtatgtgtcg ctgtacccca
     2581 cagaaggggg catgcaggat attattgact gcattcgtgc tgaactgggt gccggaacca
     2641 cgaaaaacgc cgtttttcag catatccaga gcctgccccg cggacagcag aaagaggctc
     2701 tgcttgcgtc agccgcgctg cccctgctgt tccgtccccg tgaggttcag gggacaatgt
     2761 tcggtgatgg tggtatggga ggatggcgaa atatgcaggg aaatacccct gtgacgcctc
     2821 tggtcgatgc cggatgcaat atggtgattg tgacgcatct gagtgacggt tctttatggg
     2881 atcgccaggc ttttccggac accacaatcc ttgagatccg tccccggaaa aggctgaaat
     2941 atgcaggtga tggtgacaac agcggcggtc tgctcagttt cacgttggca cataccgacg
     3001 cctggcgtca gcagggctat gaagacacga tgctgacgat ggagcatatc cggaaaccgc
     3061 tggcagcacg tcaggtactg tcccggtcag aaacggtgtt gcagaaaagt ctggagataa
     3121 cggaagaggc agatttggca ctgagaaacg cgatggcccg gattaaataa agacgctcca
     3181 aagaaacgcc accgcctgtg gtggtgtttt tcctagctgg tgttattgaa aatattttca
     3241 gatatatatt acctgagaga aagttttacc cctcacctgt caattcactc actggaaatt
     3301 taattatgca attacctgta aagttattaa tgagccttat atctctggtc agcgttattg
     3361 cacgtgccgg gaaatataaa aattatatcc gggatgaaat aaaatactgg cgatatacat
     3421 catacaaggg ggggaaattt ccggaaggtt tcactgatga gaaattttcc agcgccattt
     3481 acaacggaag aatatttaca atgaaacgtt tacataccct gatgttattt ctggcggttc
     3541 tgtttactgg ctttaacgtg gaagcagcga gcgtgaaaca agcgctcagc tgcgacccaa
     3601 acgcccgggc tgaacaacct ggagcgtgtc caacaacgta cgagttgtac gaaggtgacg
     3661 ctgcctacaa agctgcgctt gacaaagcat taaaaccggt cggactgagc ggcatgttcg
     3721 gtaaaggcgg gtatatggat ggccctggcg gaaacgtaac gccagtaacc attaacggta
     3781 cagtctggct ccagggcgac ggttgcaaag ccaatacctg ctgctgggac tttatcgtaa
     3841 cactctataa cccaaaaacc catgaagtcg ttggctaccg ctactttggt ttagatgacc
     3901 cggcctacct ggtttggttc ggcgaaattg gcgtgcatga attcgcgtat ctggtgaaaa
     3961 actacgtagc tgcggttaac taatttaagg agtctgtcat gaaaactttt atcaaaactt
     4021 tactcgttgc tgtaactatt ctgttctctg tcttcgctac ggcgaaacaa gtaaaactgc
     4081 caaacaacat caaatacgtt aatactacag aggcg
//
LOCUS       DADMFL010000217         4094 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.225, whole genome shotgun sequence.
ACCESSION   DADMFL010000217 DADMFL010000000
VERSION     DADMFL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.225"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            142..2139
                     /locus_tag="JNZ27_004078"
     CDS             142..2139
                     /locus_tag="JNZ27_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAY8666169.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2202..3479
                     /locus_tag="JNZ27_004079"
     CDS             2202..3479
                     /locus_tag="JNZ27_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAY8666170.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKK"
     gene            complement(3930..>4094)
                     /locus_tag="JNZ27_004080"
     CDS             complement(3930..>4094)
                     /locus_tag="JNZ27_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8666171.1"
                     /translation="PFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRK
                     AGVIAEANPE"
BASE COUNT          989 a          945 c         1032 g         1128 t
ORIGIN      
        1 tggtagtgtc cgctattgcc agtacacctc aacattccac catgcattcc gattaacgcc
       61 gcatagccag ttgaactttg ctactttgtg agaggtagta ccttctatcc agtgcgaatt
      121 taattaatgg aataaatgat tatgagtgaa aatgatacaa tcccaaagaa gtctacaagt
      181 cagattaaca aagcggtatt ctttacatct gctttgctaa ttttccttct tgtcgccttt
      241 gccgccgtat tcccggatgt cgccgacaaa aattttaaac tacttcagca acaaatcttc
      301 acgaatgcca gctggttcta catccttgct gtggccctga ttttactgag tgtcacgttc
      361 cttggactct cacgctacgg tgatatcaag ctgggcccgg accatgcgca gcctgatttc
      421 agctaccact cctggtttgc gatgcttttt tcggcaggga tggggatcgg cctgatgttc
      481 tttggcgttg ccgaacctgt aatgcattat ctttcgccac ccgttggcac tccagaaacc
      541 gttgcggcag ctaaggaagc aatgcgtctg acctttttcc actggggact gcacgcatgg
      601 gcaatttatg ccattgtggc gctgattctg gcgttcttca gttaccgtca cggtctgcct
      661 ttaactctgc gctccgcact ctatcccatt attggcgatc gcatatacgg acctgtagga
      721 catgcggttg atattttcgc tgttataggc acggtctttg gcgttgcgac atcactgggt
      781 tacggtgttt tgcaggtgaa tgccggtttg aaccatcttt tcggggtgcc catcaatgaa
      841 acggtgcagg taattctgat cgtggtcatc acggggttag cgacgatttc agtggtgtcc
      901 ggtctggata agggaatacg tatcctgtct gaactcaatc tgggtctggc tttgttgctc
      961 ctggcgctgg tcctgtgtct gggaccaacc gtgcttctgc tgaagtcatt tgtggaaaat
     1021 acgggcggtt atctttcgga actggtgagt aaaacgttca acctttacgc gtatgagccc
     1081 aagtcgagca actggctggg gggctggaca ttactgtact ggggatggtg gctttcatgg
     1141 tcgccgtttg tggggatgtt catcgcacgg gtctcccgcg ggcgaaccat tcgcgagttt
     1201 gtcaccggcg tgctgtttgt tcccgcgggt tttacgctaa tgtggatgac ggtgtttggt
     1261 aacagcgcga tctatctcat tatgaaccag ggggccacag acctcgccaa tactgttcag
     1321 caggatgtgg cgctggccct gtttaatttc ctggagcatt tcccgttctc ttctgtgctg
     1381 tcattcattg caatggcgat ggtcatcgtc ttctttgtaa cgtctgctga ttcgggggca
     1441 atggttgtgg atactctggc atcaggtgga gtggcaaaca cacccgtctg gcagcgaata
     1501 ttctgggcct cgctcatggg cattgttgca attgcgcttc tccttgccgg agggctaagt
     1561 gcgctgcaaa cggtgacaat agcgagtgca ttgcccttct cagtgatctt actaatatct
     1621 atatacggac ttttaaaagc tttgcgccgg gatttgacca agcgtgaaag cctgagcatg
     1681 gcgacaattg ctcctacggc tgcacgtaac ccaattcctt ggcagagaag gttacgcaat
     1741 atcgcgtatc tgccgaagcg atctcttgtg aaacgtttta tggacgacgt tatccagccc
     1801 gccatgacgc tggttcagga ggaactgaac aagcagggga cgataagcca cattagtgat
     1861 gcagtcgacg atcgtattcg tcttgaagtc gatttgggca acgagctgaa ttttatatat
     1921 gaagtgaggc ttcgcgggta tatctcaccg accttcgcgc tcgccgcaat ggataatgat
     1981 gagcagcaga ctgaacaaca tcgatattat cgcgctgagg tttatctcaa agaaggcggt
     2041 caaaattatg atgtgatggg ctggaaccag gaacagctga ttaatgacat actggaccag
     2101 tacgaaaaac acctgcactt cctgcacctg gttcgttaat agcaacatgc cgtcctgggg
     2161 gcggcaatta ttatcccggc cgcaatatga gggaatgcag aatgatttca cgctggaaat
     2221 ggatgctgaa gcagacaatt aaaaaactat ggttcagggc aacgttattc gcaattgtcg
     2281 cgataataac ggccctttta tcaattcttt ttaaatcaat gatacctgag tcggtttccg
     2341 tgaaggttgg tgcggaagca gtcgataaca ttctgaacat actggcatcg agtatgctgg
     2401 cagtgaccac attttcgctg agtatcatgg tcacagccta cggttcagcc actactaatg
     2461 tgactcccag agctacgcgt ttagttgttg aagacgtcac cacacaaaat gtactggcca
     2521 ccttcatcgg ttcttttctc ttcagtctgg tagggattat tgccctcaat atgggagctt
     2581 atggagaaag ggggagagtc attttattca ttgtcacact ggttgtcatt gccttaatcc
     2641 tcatcacatt gcttcgctgg atacagcatt tgacctctct ggggagggtt ggtgagacaa
     2701 cggcacaagt agaacaggcg gccatcgaaa catttattgc gagagcaaga aatccctgtc
     2761 tcggcggata tccatggctt gagaacaatg aacagccgaa aggaacggtt gcagtttatc
     2821 cgaagaagat tggctatgtt gaatatattg atatggtgaa acttagcaag ctgctgacca
     2881 atgatccccg tcatgtatac ctcgtggcgc agccaggcag tttcatacat ccgtccatgc
     2941 cagttttgta cctgagtcag ggccaggagt cgtcaatcaa caccgattta cttgagacga
     3001 ttattgtctc ggatgcacgt tcatttgctc aggatcctcg attttgtctt agcgtcatgg
     3061 ccgaaatagc ctgccgggcc ctttcccctg cagtgaacga tcctggaacc gccattgatg
     3121 tcattggcag aggtgttcgt atactttcca cttacgcgca gaataaatct gatgaaatag
     3181 aagtgaaata tccttcagta catgttgcac cacttcagaa taacgatcta ctggaagact
     3241 ttttctcacc tgtcgcgcgc gatggtgcca gtatgaggga gattcagata agagtcctta
     3301 aaggactgtc gatgctgagt aaaggttggc ctgagatatt tgctgaagcc gcacataccc
     3361 tggcatttga aacattagag catgcaactc gtgctgacca tatagattct gatagatatc
     3421 taataaaatc aacttattat aatttattta gtggcgaata ttctaataaa aaaaaatagc
     3481 tgcagagcaa gcaattcggt tgttggcaag tcaagggcaa agcattcgcg tgagaatggg
     3541 cgtatgcttt gtgccaaaag caaacgttgc taattctgca ctgttcagcg atggcgcagt
     3601 agcgacaggc actgccactg tccagtcggc aaaagcgaac actttgccac tgcggggata
     3661 aactgattga agctctcgcg caaaatcact tattcttttg acaataaaat gtaggagatg
     3721 ggtcatgaca ggcgaaaatc gtggccgggg aaggccgcga aagacagaaa gtacctatgc
     3781 agatactcgt aatgatctga tccgcagtgg ccttgaactg ctgacgcaga atggttttct
     3841 tgcgacggga gtcgatgcca ttgtgtcaac gacggatgaa aagtgatcca cttatatctc
     3901 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttcagc tataaccccg
     3961 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgac atgtgagtgg
     4021 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
     4081 cactgcccga acgg
//
LOCUS       DADMFL010000218         4077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.226, whole genome shotgun sequence.
ACCESSION   DADMFL010000218 DADMFL010000000
VERSION     DADMFL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.226"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..113
                     /locus_tag="JNZ27_004081"
     CDS             <1..113
                     /locus_tag="JNZ27_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8666172.1"
                     /translation="QAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JNZ27_004082"
     CDS             complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JNZ27_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY8666173.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JNZ27_004083"
     CDS             complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JNZ27_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY8666174.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JNZ27_004084"
     CDS             complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JNZ27_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY8666175.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JNZ27_004085"
     CDS             complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JNZ27_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY8666176.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1112 a          998 c          996 g          971 t
ORIGIN      
        1 tgcaggcgca ggtcgatcgc aacgatgaga tttatcgccg tcagcgggag ctgaaggaag
       61 agctgaatag tctgaaggat ttgaattcgg ttaggaattt tatcgtggaa taacagaagc
      121 tgcggcacgt cgtatgcagg aacgtgccgc ggttggctgg taaactttcg atagtgcgag
      181 tattgaatga tttctaggcg ttatcgattt tacgtatttt ttgcatgata gaattcgtac
      241 ctcctcccaa cgaccttcca tggctttccg gacttctgta ggctggcgga cgatgctgtc
      301 ctcggatgtt gtgctaattg ggtgataaca gatcctgtgt atgggcgttt ccctttgtgc
      361 gtgcgttgat gctggtaacg gtaaacgtca tgatgaacac gaccgcgaat aagacgacaa
      421 tcgtcggagc tggtgcgctg tcgataaaaa aggacagcca gacaccagtc attgacacta
      481 ttaccgatat gccagtcgcc agtaggagcg caatatgaaa gcgttgtgtc aggagtactg
      541 caatggcgcc aggcgcaatg agcagagaaa tagagagaat gatacctact gctttcagcg
      601 tcgccacaat ggtcagggaa accatgcaca gcagcccata atgcagccat ctcgtatgca
      661 aaccgcttac ctgcgcctgt tgataatcaa agctgaacag caaaaaatcc cgccatttta
      721 cgctaataac aagtgtaacc agcccggcaa taatcaccgt ctggattata tcgccgatgg
      781 ttatcccgag catgtcgccg aaaagaatat ggtcaagatg tacgtctggt ttaattgcaa
      841 tatacaagat cagacctgcg gcaaacatgc cagaaaaaac aatccccata actgtatctt
      901 gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac cgcgcagaat actccggcga
      961 cgaatgcccc tgtagccaga ggtaacccca gaatccaggt aagcacgatg ccaggaaaaa
     1021 ccgcatgact catcgcgtca cccatcagtg cccaaccttt caggaccaga aagaccgaca
     1081 gcagcgcaca gggtatggaa actaccagtg aaatcagcag tgcgtgactc ataaaggtga
     1141 actgcagtgg ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgcacg
     1201 ccgacgattt gccaaaagtc catgggtggg cgcaaataca aatgctaaaa gaaacagcag
     1261 tgtctgcgcc accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc
     1321 gccgaggaaa ctggttacac tgccaatagt tacagcaatt atgagcaggc ggggaaaacg
     1381 atcggttaac aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc
     1441 accaaccgtt tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt
     1501 taatctgccc ggatgtaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat
     1561 aagatctttc catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg
     1621 caggatatcc gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac
     1681 ggatgtcggg ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag
     1741 gccgatgatg gcatcttctt ttaagcgggt gcgttggtta agaaacagca tgctgcccgc
     1801 tgccagtccg ccggaaaaga aggctcccag agaaaacgga agccccagca tatatgcccc
     1861 cgcaacaccc gggacaatag agtgcgacag cgcatcgcca atcagcgacc agccttttag
     1921 catcagatag catgacagga aagcgcagag accgccgacc attgccgaga cccacatcgc
     1981 attgagcata tattcatagc tgaagggttc cagtagcacg ttcatcttgc ttccctctgc
     2041 accgctgacg gtcgatgtgc cacaaaaggg cgttcatcgt cagtaataat gctttcttcc
     2101 gagccgttaa gtgtgacatg gcgcagtacg ccgctaaaag ccagctccag gttctcggct
     2161 gtaaaagtgg tgtctgtcgg cccgcttgcc aggacggtgc ctttgaccat gaccgtataa
     2221 tcgcaaaatg tcgtgactga gccgagattg tgcgttgaga caagcatcgt tttgccttcc
     2281 gcgcgtaact ccctcaacag gctgatgatt ttcgcttcgg ttttcacatc aacgccggta
     2341 aaaggttcat ccagcaggat cacatcaccc tgctgtgcaa tcgctctcgc cagaaagacg
     2401 cgtttctttt gtccaccgga aagctcgccg atttgacgat ggcgaaaatc caccatatcg
     2461 acacgttcca gcgcatcggt gacaatctga cgatccctct ttttggctat acgcaacata
     2521 cccatatgcc catagcggcc catcatcacc acatcttcca ccagtacagg aaatgaccag
     2581 tcaacctctt ctgactgagg aacgtaggca accaggtttt tctgtagcgc ctgtcgcgtg
     2641 ggaataccca gaacagatat ctttccgctg gtcagacgca caaatcccat aatcgcttta
     2701 aacaacgtcg attttccgga accgtttacc ccaaccagag cggcaataga tccacttggt
     2761 actgtgaagg atgcatcgcg cagtgctgtg tgcccgttac gccaggtgac agtaacatca
     2821 ttaacgacaa tgcctgcaga ttgcatcatt atttctccct tttcccggct ttaattccct
     2881 gtaccagcgt actggtagta accttaagaa ggtcgatata cgtaggtacc gggccgtttt
     2941 ctgtgctcag ggaatcgaca tagagcacac cgccgtagtg tgcgccggtt tcacgcgcaa
     3001 cctgacgcgc tggtttatcg gaaatcgtac tctcgctaaa gactgccggg atatgatttt
     3061 tcttaactat atcaacaacc ttacgtacct gctgcggtgt tccttgttga tcggcattaa
     3121 tcggccacag ataaagctct tttagcccca aatcgcgtgc gagataagaa aaagcccctt
     3181 cactggtgac catccatcgc tgattctcag ggagttccgt aatctgctta cgcagggggg
     3241 caagggtttg ggtaatcttg gctttataag tatcggcatt acgttggtag gtttgtgcat
     3301 ttgccggatc gtattttatc aacgcatcac gaatattatc gacgtaaatc agagcattat
     3361 ctggcgacat ccatgcatgg ggattaggtt tgccctcata ggggccttcg gtgatcccta
     3421 ctggcgtcac acccgaagag acaattactt ctggaacccc attgagatgc tggtaaaagc
     3481 gttggaacca caattccaga ttcataccat tggcgagaat cagttgtgcc ccctgcgcac
     3541 gtttaatatc gccaggggta ggctgatact catgaatttc tgcaccaggc ttggttatgg
     3601 atgagacttc tgcagcatct ccagccacgt ttttggccat atccgcgatg atggtaaatg
     3661 ttgtaatgac cttgaatttt tcagttgcac ttgcctgaaa tgcgatcgag caggtgaggg
     3721 cgaggcaccc caagagcatg gttacttttt ttattgagtg cataatttag tacctatagt
     3781 gagcaaaatt agaacaaaca tagcaacggc tatgttttat agcataacct atacaaaatg
     3841 aaaataatta tcattaacag ctttgaggcc taaaacaggg attaggttgt ttcctgctgg
     3901 cgacatgata ttgaggaaaa aagtagagca gctattaggc tttgaaatga ctttatgagg
     3961 aaaattgttg atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa aggggcaaaa
     4021 aagagtgggt tatcgtagct tattgttgtc gctgataatg tttaacatat tgaaaaa
//
LOCUS       DADMFL010000219         4010 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.227, whole genome shotgun sequence.
ACCESSION   DADMFL010000219 DADMFL010000000
VERSION     DADMFL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.227"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(247..1605)
                     /gene="plaP"
                     /locus_tag="JNZ27_004086"
     CDS             complement(247..1605)
                     /gene="plaP"
                     /locus_tag="JNZ27_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY8666177.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="JNZ27_004087"
     CDS             complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="JNZ27_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY8666178.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1873..2802)
                     /locus_tag="JNZ27_004088"
     CDS             complement(1873..2802)
                     /locus_tag="JNZ27_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8666179.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2848..3672)
                     /locus_tag="JNZ27_004089"
     CDS             complement(2848..3672)
                     /locus_tag="JNZ27_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8666180.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="JNZ27_004090"
     CDS             complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="JNZ27_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY8666181.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1091 a         1038 c          935 g          946 t
ORIGIN      
        1 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
       61 gaaaacataa attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga
      121 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga
      181 taaaaaagaa taaaaaaacc ggagccaaat gtttattcga ctccggttta ttattagaaa
      241 agatgattat gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca
      301 agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc
      361 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
      421 gaagtgatct ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga
      481 caggttaaca aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac
      541 caggtcgaag ttgattgcca gcagggcaat cgagccaacc aggatgatgt tcatcgccgg
      601 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc
      661 cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt
      721 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
      781 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
      841 gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg
      901 cgggatcacg cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc
      961 ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc
     1021 agaccagaac ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
     1081 aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt
     1141 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc
     1201 caccacaaac atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag
     1261 cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa
     1321 gccgacagtc gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata
     1381 gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc
     1441 ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag
     1501 cgtcatcggc tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt
     1561 acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta
     1621 cggtgataac cgtcgcgtaa gcaaaaaatt tgccccattt ttttggattc ctcagcgaca
     1681 acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct tggtatgaat
     1741 gattggtttg aagcaaaaaa taaccgacgc taattaaacg tcggttttta gtcatttttt
     1801 gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat
     1861 aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa caccggcgat
     1921 cgcactattt gctaaaattt catctcgcca ccagccgctg gcaagccctg cggtctgttc
     1981 attccagcca atccagacag gttcaaagag agtttgggcg accactttct tctcgattaa
     2041 cgcgccacta tcgagaaaac gttgcgccag ataccgtggt aaatagccgc agccaaggcc
     2101 gctaatttgc agctccagtt tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc
     2161 gagaagttgt gaggccgtag aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg
     2221 atatcgttta atgatacgac ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg
     2281 cgcgaccgca aagacttgtt caagatcgcc caaccgcgag aaaccaaact cactggaaga
     2341 tggtggctca tgcatcgcgc caacgataat atccgctcgc ccctgagtca gggcatccca
     2401 ggaaccaccg agtacgccat tgataaactt caggcgcgtc acgctatgat gttgataaaa
     2461 agcttcaatg agaggcgcaa gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag
     2521 ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc gcacggtatg
     2581 cagaacttcc cgccctttct ctaataacat ttttccggtg cgggtgaatt tagcgcggtg
     2641 tccgctacga tcaagcaatt gaatatttag gtcgctttcc agcttgtgaa cggtgtaact
     2701 cagagcggac ggcgtcttgt agagtttggc tgacgccgcc gcaaaactgc cctctttttc
     2761 tatggcatcg aggatcatca acacatccag cagtggtttc atacacgccc ccttgcagtg
     2821 catgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg gatcgggata
     2881 ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct tacctttgcc
     2941 gctgtccaga ctatttctga actgcggagg ttgcagcccc agtaaacggg ccatctgcgg
     3001 atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt gtccgccttt
     3061 aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga ctaaattaac
     3121 accatgttca ccatcagggg cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg
     3181 tccgaccagg cccgcaagac gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca
     3241 gtcttcgagt tcttctaata ctcgtccact gttggttacc ggattacgcg gggtagtttc
     3301 tttaaccgtg ccttgcgcgt cgccatagac agatgttgag ctggtaaaaa taattcgggg
     3361 aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat agaactcatc
     3421 gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat ccataagggc
     3481 atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat agctatcaat
     3541 gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca
     3601 gcctcgcgct gaaagtgaca tcgccagcgg catacctaac caccctaacc cgacaattgc
     3661 gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac gctagcgtat
     3721 caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga catgctgcaa
     3781 tgagcagtga atcatcagta accgcgtata ccagacggtg ctcctctgta atgcgtcggg
     3841 accagaaacc tgacaaatta tgtttcaggg gttctggttt ccccttacct tcaaatggtg
     3901 ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta tctgtttcct
     3961 gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
//
LOCUS       DADMFL010000220         3999 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.228, whole genome shotgun sequence.
ACCESSION   DADMFL010000220 DADMFL010000000
VERSION     DADMFL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.228"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            824..1579
                     /locus_tag="JNZ27_004091"
     CDS             824..1579
                     /locus_tag="JNZ27_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY8666182.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIR
                     NMVEELNGSKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAG
                     YSVHLRAKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKANGLPAQKLMT
                     ILKKERKLFRVHPFSGEWSVNPNYVEYFRSIKPTDHRFNPNGINIRFNAKGLEFFSRP
                     ENVLKMHRRVIAVHGSDAAKQQHIQAVAKLEGR"
     gene            1586..1810
                     /locus_tag="JNZ27_004092"
     CDS             1586..1810
                     /locus_tag="JNZ27_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666183.1"
                     /translation="MTNNTNDTNTTSTASIKIDPRTPEGRKALRLMVIPTKALIATLG
                     LPAKENRQYYSKAALCLMAVDAGLTPRDFM"
     gene            1988..2398
                     /locus_tag="JNZ27_004093"
     CDS             1988..2398
                     /locus_tag="JNZ27_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666184.1"
                     /translation="MSISYRKLDITLSADKETVLVFGQELSTKYFTEIVVTTMLNSTG
                     SDMAVSNRILNDIHAAGLDAGDYGKYSRWWAQSNAQERQEAERRRKEAKEHQERLAAI
                     HATPEEIAKAVAERKAREEALIKRFGNKGAAFGL"
     gene            2489..3610
                     /locus_tag="JNZ27_004094"
     CDS             2489..3610
                     /locus_tag="JNZ27_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666185.1"
                     /translation="MAITNQINKASSLASLRQPQRDKDGQIIKGSVWGTDISRTDYVQ
                     MTNGQDAQVLNVGFTDRSGRPMAFGNDSHDYVQALEDSLDETFNDGDFRTAVMENVFP
                     CEFRPYGTDPTPIRRQALQNRTVRFKHNGAFNPAEDAPSTTVIKGASVTVTPVLNPET
                     NKTGILCSAATHVSDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTPDLQI
                     IEAAPLSGKPSDQAEDLLDTLALNLPVELGNTLSDYAVLVPESLEAILDRAAQRAGHE
                     DISELLGCTVGGYAGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLEL
                     ISVVDVLATGSVKVKAGEFDVEEDASFPLVHVIRFTTPE"
BASE COUNT         1132 a          920 c          923 g         1024 t
ORIGIN      
        1 atagggagaa gggacacaaa aagttttctc ttctaggaca actaaccgta acttatcgca
       61 tccttttcag gggcgggatg accaattaca gacaccaagc gttctactta aagtgaatgc
      121 ttcctgtgtg ctctgggtca gtcgataagt gaatagcaat aagacacaat catcttaatt
      181 atggtctcta gtccctacag gggcttaact taaagttaac ttaaagacac aattaaagat
      241 taacttatag tattaaataa aagatttaat taaagacaca attaaagatt aacttatagt
      301 attacttaaa gttaacttaa agacacaatt agagattaac tttaagtatt aaataaaaga
      361 tttaattaaa gaccaattaa agattaactc taagggaata ccttaagggg tgatgatatt
      421 tataataatc aatcaccaaa cctataggtc ttctctaggg tctctctata ggtcttctct
      481 agggtctctc tatggtctct ctagggtctc ctctagggtc tcctctaggc attaccccca
      541 gaagctaact ctatggtcct ctatggccct gtacgtcatt ctttaggatg ccctgtagtt
      601 tcgtatagcc tgatgcctag agagacccta gagagaccct agagtggctc ctagagagac
      661 cctcccagta gctccgcaca gctacatcgt catcatcata gtattcaatc tcaaagtcat
      721 tctgtttgca tcctgttgtc ctgctggtct cccttcgctt ggggactttg agtaggtcag
      781 tgggggtgcg acttcagggt gcaaacaaaa aggaaatcaa gcgatgtctc aacaaccaca
      841 acaaccacaa caagaaatca gcatcattaa cctcgaccag cttgtgtcca tgacctcagt
      901 tgaaattgct gagctgacag gaaaagaaca taaacacgtt ttacgagata ttcgtaatat
      961 ggtcgaagag cttaacgggt ccaaaactga gcactgctca actttgagca gcgagcttaa
     1021 cgggtccaaa tttgggctcg ttggcgagga agtatacaag gacgcgaaag gtgagagccg
     1081 aacaatgtat cgtctcgacc gtaagcatac ttttattctg gtcgctggct attctgttca
     1141 cctacgcgcc aagtgttacg accatattca gacgttagag cgtcgagttc ttcaactgga
     1201 agaccagaag aaacgagcag ccattcagtc agctaatcgt cgaggcgtga cttggggcga
     1261 ttactgcaag gccaatggtc tacccgctca gaaactgatg accatcctta agaaagagcg
     1321 caagttgttc cgagtacatc ctttcagtgg cgagtggtca gtcaatccaa actatgtgga
     1381 gtactttcga agtatcaaac ctactgacca tcgattcaac ccaaacggta taaacattcg
     1441 gtttaacgct aaggggctgg agttcttcag ccgacctgaa aacgtactca agatgcacag
     1501 aagggtcata gcggttcatg gaagcgacgc tgctaaacag caacacatac aggcagtagc
     1561 aaagctggag gggaggtaat tcatcatgac taataacact aatgacacca acaccactag
     1621 cacagctagc atcaagattg acccaaggac accagaaggc cgtaaggcgt tacgcctgat
     1681 ggtgataccc accaaggcac tgatagctac tctgggtctg cctgccaagg aaaaccgcca
     1741 gtattactct aaagctgccc tctgtctcat ggcggttgac gcagggctga ccccaagaga
     1801 tttcatgtaa ttaaccaata ctagcaccac tagcaccact agcaccacta gcaccactag
     1861 caccactagc tatccttttg aactgaccga gatgcgggga gacgccgtag gaatacctat
     1921 ggaagaccga taagttcaat ctttcaaaac atcttttcaa aacatctttt caaaaggaga
     1981 aacaaatatg tctatttctt atcgcaaact ggatatcaca ctgagtgctg acaaagaaac
     2041 tgtactggta ttcggtcagg agctatccac taagtatttc accgaaattg tcgttacaac
     2101 catgctgaac agtacaggct ctgacatggc ggtctcaaat cgtattctta acgatattca
     2161 cgccgctggt cttgatgcag gtgattacgg caagtattcc cgctggtggg ctcaaagcaa
     2221 cgcacaggag cgccaagagg ctgaacgccg acgtaaagaa gctaaggaac atcaagaacg
     2281 tctggctgct attcatgcga caccagaaga gattgctaag gcggtcgctg aacgtaaagc
     2341 ccgagaggaa gcattgatta aacgcttcgg caataaaggg gccgctttcg ggctgtaact
     2401 gatcacttta ggtggcgctg cttggattct gagataagac caatggtgtc accagttact
     2461 ttttacgtag tataaggaga tatttatcat ggctattaca aatcaaatta acaaagcatc
     2521 ctcactggct tctctgcgtc agcctcagcg agataaagac ggtcaaatca ttaaagggtc
     2581 tgtatggggc actgacatct cacgcactga ctatgttcag atgaccaatg gtcaagacgc
     2641 ccaagttctc aacgtaggct ttacggaccg ctcaggccgt ccaatggcct ttgggaatga
     2701 ctcacatgat tacgttcagg cgctggagga ctctttggat gagaccttca atgatggaga
     2761 cttccgcact gctgtaatgg agaatgtctt cccttgtgag tttcgcccat acggcactga
     2821 ccctacgcct attcgccgtc aggctctcca gaatcgcacc gtccgcttta agcataatgg
     2881 cgcgtttaat ccagctgagg atgctccgtc taccacagtc atcaaagggg caagcgtaac
     2941 tgtcacccca gtgttgaacc ctgagaccaa taagacgggc atcctgtgtt ctgctgctac
     3001 tcatgtgtct gacctcgata tgatgggcgg ttggaccgag ggggcattaa tccagactgt
     3061 atgcgatatg caggttcaat actgccgcca gatgttcgcc gctgtggtcg atgtcctgaa
     3121 agatacgcca gaccttcaaa tcattgaggc agccccacta agtggtaaac cgtctgatca
     3181 agctgaagat cttctggata cattagcgtt gaatcttccg gtcgagttgg gaaatacact
     3241 gagcgattac gctgtattgg tccctgagag ccttgaggca atcttggacc gagccgctca
     3301 acgtgcaggt catgaggata ttagtgagct attgggctgc actgtaggcg gctacgctgg
     3361 cgatgataca ggtgtctatc tcctgcctaa gcggttcgct tctatctctt tcaggtcaac
     3421 taaagacgct aagacggtag atgtcaaagt gacccgaaat agtaacactg ctgggtatga
     3481 ccttgagtta atcagtgtgg tcgatgtgtt ggctactggt tccgttaagg tgaaagcagg
     3541 tgagtttgac gttgaggaag acgcctcttt cccgttggtc catgttattc ggtttactac
     3601 cccagagtaa ctataaaccc tgagtgaaca taaggagtac atccatgagc ttacaggagt
     3661 tgtatatggt cgtcctctgt gtcctcttgg tctaccagat gctctacagg gagtgataga
     3721 tactcgctat tgctctggtg cttacaggtc ttcactttgc ggcctgtggg ggcagtaggt
     3781 tatgaccttg gattaaaaaa tctcacaaaa atctctgtag gtatttaata gataaaggtc
     3841 agcgacttcc cccataggcc cctttcaagt ctggacgatt ggggccactg tcttaaagtt
     3901 aggacttcgg gacttgacca gagataaatt aatgtgtaac cataactgta accaaaagga
     3961 ttcatcatcc tttaagaaac aattggttag tgagtgatt
//
LOCUS       DADMFL010000221         3991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.229, whole genome shotgun sequence.
ACCESSION   DADMFL010000221 DADMFL010000000
VERSION     DADMFL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.229"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..92)
                     /locus_tag="JNZ27_004095"
     CDS             complement(<1..92)
                     /locus_tag="JNZ27_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666186.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(345..1694)
                     /gene="lysC"
                     /locus_tag="JNZ27_004096"
     CDS             complement(345..1694)
                     /gene="lysC"
                     /locus_tag="JNZ27_004096"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY8666187.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFSVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2219..3868
                     /gene="pgi"
                     /locus_tag="JNZ27_004097"
     CDS             2219..3868
                     /gene="pgi"
                     /locus_tag="JNZ27_004097"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY8666188.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1011 a         1051 c         1011 g          918 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg ggggagtgcc atgatggtgc cctcagtgag ccagagagtt
      121 cagtgtacgg gaattcgttt gcgagcagaa ctgataatat tcattttgcc ttctcttaac
      181 tatttatctc tttcgataat taattagaga tgcagataaa aaaattaaag gcaattattc
      241 tccgataaag acgttggtta acagagtaca ggctcgttta ttgatgagca tagtgacaag
      301 aaaatcaata cggcccgaaa tatagcttcc aggccataca gtatttactc aaacaaatta
      361 ctatgcagtt tttgcaccac ctgctcggca tcttcgccgg gcaccaggaa gcacaggtta
      421 tggctggatg cgccgtaaca aatcatgcga atgttgaacg gttccagtac gctgaatacc
      481 tctttgccaa cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac cagcgccaga
      541 ccttcttcca cctccacccg gcacagtgcg gaaagctcca tcagcagaga ttgcgtcagc
      601 aacgtatcgc cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac gctcacttct
      661 gacgtggtga ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa aacttccgcg
      721 aggaagccgc gagaatgcag catattcagg ctgtgcaaag tgagcagagt ctgattgcga
      781 cgaagcgcca gcgcgcggaa cagcggcgga ttttcagttt tattgcacac cagcgtacca
      841 cctgcgcgtg ggtctttgct ggagccgaca aagaccggga tatcgctgcg tactgcgggt
      901 agcaacgttg ccggatgcag tacttttgca ccaaaagttg ccatctctgc cgcttctgca
      961 aacgcgattt catcaatgcg ttttgctgcg gaaactacgc gtggatcggt ggtgtagatg
     1021 cccgggacgt cggtccagat atcaacacga gatgcgtgta aagcctccgc cagcaaggct
     1081 gccgtataat cgctgcctcc acggccaagc gtcgttgtac gacctttatt ttcgctaccg
     1141 ataaatccct gggtgatcac taagccttca ttgaggcgtg ggagcagctg cagcgcggcc
     1201 agttccgcca gcgcggctac atctggctct gcacgaccaa atcggtcgtt ggtacgcatc
     1261 actttacgta catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat ttcgacaaac
     1321 agcagggtcg acatcagctc gccgtggctg accagctcat ctgtcagcgc cggagacgtt
     1381 gccagcgccg ccgcttctgc cagaacagta atgttctcca gcagacgttc aatctcttca
     1441 cggataacgt tcgggtaacg cagacgttcc agaatggcaa actggatgtt gcgaatagcg
     1501 tcgagttttt cgaatcgctc gccaggttcc agcccttccg ctaaagcgac cagcagatta
     1561 gtgataccag cagaagccga gaggactact aaacgcacgt tggcatcaga aagcacaata
     1621 tcagcgctgc ggttcatggc gtcaaaatca gctacgctgg taccgccaaa tttggagaca
     1681 acaatttcag acataactac ctcgtgtcag gggatccatt ttcagccttg gcacaaggga
     1741 agagcggaag acgggtgggc gcagagcgat acttcgctac tattttcacc cagaagtgct
     1801 ccaccacttg cgaaacgccc gactgcgaac gcttctggtg acaacccagg ggattcagcc
     1861 cctgtagccg atgatgaacg tggccagccg ttcaatcacc tcggcgatgc accccctcag
     1921 gtgttatcac aggactggct cctccaacac cgttacttgg gcaacgcgcc tcttctggcc
     1981 tgcgctagcg caggtagtac atttataaat aaagggtgag cggggcggtt gtcaacgacg
     2041 gggtcatgcg gatttttcat ccactcctgg cggtcagtag ttcagctaat aaatgcttca
     2101 ctgcgctaag ggtttacact caacattaca ctaacggcac taaaaccatc acatttttct
     2161 gtgactggcg ctacaatctt ccaaagtcac aattctcaaa atcagaagag tattgctaat
     2221 gaaaaacatc aatccaacgc agaccgctgc ctggcaggca ctacagaaac acttcgatga
     2281 aatgaaagac gttacgatcg ccgatctttt tgctaaagac ggcgatcgtt tttctaagtt
     2341 ctccgcaacc ttcgacgatc agatgctggt ggattactcc aaaaaccgca tcactgaaga
     2401 gacgctggcg aaattacagg atctggcgaa agagtgcgat ctggcgggcg cgattaagtc
     2461 gatgttctct ggcgagaaga tcaaccgcac tgaaaaccgc gccgtgctgc acgtagcgct
     2521 gcgtaatcgt agcaataccc cgattctggt tgatggcaaa gatgttatgc cagaagtcaa
     2581 cgcggtgctg gagaagatga aaaccttctc ggaagcgatt atctccggtg agtggaaggg
     2641 ttataccggc aaagcaatca ctgacgtagt gaacatcggg atcggcggtt ctgacctcgg
     2701 cccgtacatg gtgaccgaag cactgcgtcc gtacaaaaac cacctgaaca tgcactttgt
     2761 ttctaacgtc gatgggactc acatcgcgga agtgctgaaa aaagtaaacc cggaaaccac
     2821 gctgttcttg gtagcatcta aaaccttcac cactcaggaa accatgacca acgcccatag
     2881 cgcgcgtgac tggttcctga aagcggcagg tgatgaaaaa cacgttgcga aacattttgc
     2941 ggcgctctca accaatgcca aagccgttgg cgagtttggt attgatactg ccaacatgtt
     3001 cgagttctgg gactgggtcg gcggccgtta ctctttgtgg tcagcgattg gcctgtcgat
     3061 tgttctctcc atcggctttg ataacttcgt tgaactgctt tctggcgcac acgcaatgga
     3121 caagcatttc tccaccacgc ctgccgagaa aaacctgcct gtcctgctgg cgctgattgg
     3181 catctggtac aacaatttct ttggtgcgga aactgaagcg attctgccgt atgaccagta
     3241 tatgcaccgt ttcgcggcgt acttccagca gggcaatatg gagtccaacg gtaagtatgt
     3301 tgaccgtaac ggtaacgttg tggattacca gactggcccg attatctggg gtgaaccagg
     3361 cactaacggt cagcacgcgt tctaccagct gatccaccag ggaaccaaaa tggtaccgtg
     3421 cgatttcatc gccccggcta tcacccataa cccgctctct gatcaccacc agaaactgct
     3481 gtctaacttc ttcgcccaga ccgaagcgct ggcgtttggt aaatcccgcg aagtggttga
     3541 gcaggaatat cgcgatcagg gtaaagatcc ggcaacgctt gactacgtgg tgccgttcaa
     3601 agtgttcgaa ggtaaccgcc cgaccaactc catcctgctg cgcgaaatca ctccgttcag
     3661 cctgggtgcg ttgattgcgc tgtatgagca caaaatcttt actcagggcg tgatcctgaa
     3721 catcttcacc ttcgaccagt ggggcgtgga gctgggtaaa caactggcaa accgtattct
     3781 gccagagctg aaagatgata aagaaatcag cagccacgat agctcgacca atggtctgat
     3841 taaccgctat aaagcgtggc gcggttaatc atcatgcgtg cctgatgccg atatgtaggc
     3901 cggataaggc gtttacgccg catccggcaa ccggagcctg atgccaatat cgacattaat
     3961 gcatagtatg aggagttaaa taaccttaaa a
//
LOCUS       DADMFL010000222         3807 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.230, whole genome shotgun sequence.
ACCESSION   DADMFL010000222 DADMFL010000000
VERSION     DADMFL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.230"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..203)
                     /locus_tag="JNZ27_004098"
     CDS             complement(<1..203)
                     /locus_tag="JNZ27_004098"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY8666189.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2512)
                     /gene="xdhA"
                     /locus_tag="JNZ27_004099"
                     /pseudo
     CDS             complement(214..2512)
                     /gene="xdhA"
                     /locus_tag="JNZ27_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2927..3682
                     /locus_tag="JNZ27_004100"
     CDS             2927..3682
                     /locus_tag="JNZ27_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY8666190.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          951 a          941 c         1002 g          913 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccc gtagaagcga tggagtgcct gtgagatgca taagcgccgg
     1561 tgttagacag aacatccaga ctataacctt tcaaggttcc gtcgcggttc acgcccattt
     1621 gcccgtcaat agtaaaagcg tggcgggtac gggttgcgag gaaacactct tcacggctaa
     1681 gggaaacttt caccggaatg ccgccaagct tgctggtcag gaatgccgcc attggctctt
     1741 ccagtacatc ctgtttatta ccaaaaccgc caccgacaaa cggtttgatg actcgtacgc
     1801 atgaccaggg aatatccagc gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg
     1861 tgctggaaac gatggtaatt cgcgagtcat cctccatcca cgccagcgat gttacgcttt
     1921 ccatgtgaca atgttgaata acgggagtct gatagtgccc ctgtacctgg tagtcggcgg
     1981 catcgattgt ttgttggaca ttgcccgtcg acatcgtgct ttgtttcagt aaattgccgc
     2041 cgttatggat tggtgcagcg tcttctgtca gcgccgcttc tggcgtggtg ataacgggta
     2101 attcttgcca ctcaatgctg accaattgcg ccgctttttc tgccgtgagt tcatcgcggg
     2161 ccacgacgat ggcaacggcg tcaccatgat gacgaacatg gcgagttagc agtgcgcgat
     2221 cggcggtatc gcgcttgttt tcgtcaagtg tccaggcatg cccagctgta gcgaatggaa
     2281 tatcaggcac atcttcccaa gtaaaaatcg ccagtacgcc cggtaaactt ctggcttgtt
     2341 catcattaat acttacggca taaccatgtg cgatagggct acgtacatat ttcgcgtaac
     2401 acatgcccgc cataacataa tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg
     2461 aatcgacgcg catgcatgat tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc
     2521 cctcgatttg aacatttggt tcttatggca aagatttagt ctaagcaggc aagatttacg
     2581 ccagaaacga cgataagcgt taggggagga aggaagtgtg atgacgttaa aaaaacacag
     2641 atatgatacg aaacaggact ctgaataggg acagtgatac gtatcaatat gagtggcgat
     2701 aaaatcaatg tgataaatca ggattattac gcatcaggat gggaaatagg gtaaaaagtc
     2761 aggaacttcg atacctctac agcgaatatt ttgctgatga tacttccctt tgccaaatga
     2821 aattaacctc tttctctatt accacgtttt tccagaagca agagattggg tatcaagagg
     2881 ctggctgcta tgatatggcg tcttgttttt taagcgagga aagattttga gtgcgggacg
     2941 cctgaataaa aaatctctgg gtatcgtgat gtttttatcg gttggactgc tattggcggg
     3001 ctgttcgggt agcaaatcat ccgatacagg aacgtattcc ggctccgttt acaccgtgaa
     3061 acggggggat acgctatatc gtatttcgcg caccacggga accagcgtaa aagagctggc
     3121 gcgactgaac ggcatttccc ccccttacac cattgaagtt ggtcagaaac taaaactggg
     3181 tggggcgaaa agtagcagta gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc
     3241 atcggttaca ccgtcatcag cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg
     3301 ttggttatgg ccaacgacag ggaaagttat catgccgtat tcgacagcag atggcggcaa
     3361 taaagggatt gatatctcag ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa
     3421 ggtggtgtat gtgggcaacc agctgcgtgg ctacggtaat ctcatcatga ttaaacacag
     3481 tgaagattac attacggctt acgcccataa tgacacgatg ctggtaaata atgggcaaag
     3541 cgtgaaggct gggcaaaaaa tcgccactat ggggagcacg gatgcggcat ctgttcgcct
     3601 gcatttccag attcgttacc gtgcaacggc aattgatccg ctacgttact tgccgccgca
     3661 gggcagcaag ccaaaatgct gatggcgaat taatcagcag tcagcagcgc ggcacttgct
     3721 aaggagagcg taaggtttat aatgccttac gcatctcgaa gcgggcgtag ttcaatggta
     3781 gaacgagagc ttcccaagct ctatacg
//
LOCUS       DADMFL010000223         3796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.231, whole genome shotgun sequence.
ACCESSION   DADMFL010000223 DADMFL010000000
VERSION     DADMFL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.231"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..115
                     /locus_tag="JNZ27_004101"
     CDS             <1..115
                     /locus_tag="JNZ27_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="catalyzes the formation of
                     2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline from
                     5-hydroxyisourate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY8666191.1"
                     /translation="ESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            224..1228
                     /gene="msrP"
                     /locus_tag="JNZ27_004102"
                     /pseudo
     CDS             224..1228
                     /gene="msrP"
                     /locus_tag="JNZ27_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            1229..1863
                     /gene="msrQ"
                     /locus_tag="JNZ27_004103"
                     /pseudo
     CDS             1229..1863
                     /gene="msrQ"
                     /locus_tag="JNZ27_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            2121..2771
                     /gene="zinT"
                     /locus_tag="JNZ27_004104"
     CDS             2121..2771
                     /gene="zinT"
                     /locus_tag="JNZ27_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY8666192.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1061 a          843 c          834 g         1058 t
ORIGIN      
        1 tgaaagtttc ttccctgaga ttcccgttga atttcatatt aataaagtga acgagcatta
       61 tcatgtgcct ttgttactca gccaatatgg gtattcaacc tatcgtggca gttaatgcgt
      121 aaatatgtaa aagctgatct ctgctactgt tagggaggtg actccctgac agattaacag
      181 taaacagctc ttgttggtta acgacaaaaa agtgtgatgg cttatgaaaa agaatcaatt
      241 tttaaaagaa tcagatgtta cggccgagtc ggtattcttt atgaagcgtc ggcaggtgtt
      301 aaaagcactg ggcatcagcg cagctgcatt ttctttgcct cacgctgcgc atgccgatct
      361 gcttagctgg tttaaaggga acgatcgtcc gcccgccccc gccggaaaac cgctggagtt
      421 cagcaagcct gccgcctagc aaaataacct gccactgacg ccagcagata aagtctccgg
      481 ttataacaac ttctatgaat tcgggctgga taaagacgat cccgcggcta atgctggtag
      541 cctgaaaacc gatccatgga cactgaaaat cagcggcgaa gtggcaaaac cattgaccct
      601 cgatcatgat gatttaaccc gtcgcttccc gctggaagag cgtatttatc gtatgcgctg
      661 cgtggaagcg tggtcgatgg tggtgccgtg gattggtttt ccgctacaca aattgctggc
      721 gcttgccgaa cccaccagca atgcgaagta tgtcgctttc gaaacgattt atgcgccgga
      781 acagatgcct ggccagcagg accgctttat cggcggcggg ctgaaatatc cttatgtcga
      841 aggattgcgt ctcgacgaag caatgcatcc gctcacactg atgaccgtag gtgtttatgg
      901 caaggcgtta ccaccgcaaa atggcgcgcc ggtgcgactg attgtgccgt ggaaatatgg
      961 ctttaaaggg attaaatcga tcgtcagtat taagctgacc cgcgagcgtc cgccaaccac
     1021 ctggaatctg gcagcgcctg acgaatacgg tttttacgcc aacgttaatc cgcatgttga
     1081 tcacccgcgc tggtcacagg ctaccgaacg atttattggt tcaggcggca ttctcgatgt
     1141 tcagcgccag ccaacactac tgtttaatgg ttacgccgac caagtggcat cgctgtatcg
     1201 tggcctggat ttgcgggaga atttctaaat gcgtctgaca gcaaaacagg tgatatggct
     1261 gaaagtttgc ctgcatcttg ccggattgtt gccgtttctc tggctggtct gggcgatcaa
     1321 tcacggtgga ctgggtgccg atccggtgaa agatattcag cattttaccg gtcgcactgc
     1381 actgaaattt ttgcttgcgg ccttgttaat cacacctctg gcacgctacg caaaacagcc
     1441 gttattgata cgcactcgcc gcctgttagg cttatggtgc tttgcctggg cgacactgca
     1501 tttaaccagt tacgcattgc tggagttggg cgtgaataat ctggcgttat taggtaagga
     1561 gttaattacc cggccttatt taacgttagg aattatcagc tgggttattt tgcttgcttt
     1621 agcgttcact tcaacccagt cgatgcagcg aaaactcggc aaacactggc aacagttgca
     1681 taacttcgtc tatcttgtcg cgatcctggc cccaatacat tatctgtggt cagtgaagat
     1741 tatttcaccg cagccactca tctacgctgg gctggctgta ctgctttagc cttacggtat
     1801 aagaagttgc tttccctttt caaccggtta cgcaagcagg cccacaacaa attatctcta
     1861 taacttcata ttgccgacaa acttcgcgtt atcccggccg gatgcggcgc gagcgcccta
     1921 tccggcccac acagatcgca tcaagttatg tttcgtttgt catcaacctt tcatcaccag
     1981 aataatttat tttatttcaa taagatagtt caatacacct gagaatacca tgctctcatt
     2041 tcctaatgag ttgttgcatt ttttcatatg ttacaatata acattacaca taatgtacat
     2101 taactctgga ggaaattgtt ttggcgattc gtcttcataa actggctgtt gctttaggtg
     2161 tctttattgt tagcgctcct gccttttcgc atggtcatca ttctcatggc aaacccttaa
     2221 cagaggtcga acaaaaagct gctaatggtg tttttgatga taccaatgta caaaaccgaa
     2281 cgctcagtga ctgggatgga gtctggcaat ccgtttaccc tttactgcaa agtggcaaac
     2341 ttgatcccgt ctttcagaag aaagcggatg cagataaaac caaaacattt gctgaaatta
     2401 aagattatta tcgcaaaggt tatccgacag atatcgaaat gattggcatt gaggacggca
     2461 ttgtcgaatt ccatagaaat aatgaaacaa catcctgtaa atatgattac gatggataca
     2521 aaatactcac ctataaatca ggcaagaaag gcgttcgtta cttattcgaa tgtaaagatc
     2581 ctgaaagcaa agcccctaaa tatatacaat ttagcgatca tataattgca ccacgaaaat
     2641 catctcattt tcacattttt atgggtaatg attcccagca atctttattg aatgaaatgg
     2701 aaaactggcc aacgtattat ccctatcagt tgagtagcga agaagtggtc gaggagatga
     2761 tgtctcattg atttttttgc gctgacttca gttatttaac agaacagtgc ctggtagtaa
     2821 caacaataat ttaccaagac aaataagtaa gacaaactca gacacgctca tacttgagtc
     2881 gtgtcttttt tattatgtct gtagaaaaaa actgagaaaa aggcgaacaa taaatacaca
     2941 actcagccat acagactggc agcgcaaatg tttccaggag caagtcatct gtcatggtaa
     3001 agctaaccag aaatataggt taattttggt gtttaaacct tttttacttg ttagtatgat
     3061 acaaaggctt tcaaaaaagc tgggaaattc aacaactctt ggaagttatg gactttgtcc
     3121 cgcggcttta atctgacggc cgcgttcctt ttttggctca attattgacc ccttctctac
     3181 gcaacttcag ttcccgccag caactttgcg gcagtttcat aggatcaatg tctaaaagaa
     3241 taatggtgac cgataagaaa acgactgaat aactgtagat tttcgctcga aaccttcctg
     3301 tcagctccat agcgaatcaa gcgctgaatg tcacagtatc gaacagaaaa cagtgacgat
     3361 ctaacccttc aagaatattc tacgattgtt ctgtttagga aaagcaaggc gggaagtcgg
     3421 gagataagtc attgataaag tggcggtgag agggggattt gaacccccgg tagagttgcc
     3481 cctactccgg ttttcgagac cgatgataca gagtcgaaaa atcaaagagt taaaaatttt
     3541 tcttggaaaa aatgtcttaa aatcgtacac gaaaaatcaa tgaattacat aacattaaag
     3601 aagaatattc caagccttct tgagtccacc acctgttttc aggtgtcatt cagacgtgat
     3661 ttgccccaga ttgggaaagc acgctttttt ctcagcttat tccgttttgc ctgtgtgttg
     3721 aataattcca ctgcagttat ataaaatcgg taacggctgg aaatcattca atactcgcac
     3781 tatcgaaagt tcacca
//
LOCUS       DADMFL010000224         3680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.232, whole genome shotgun sequence.
ACCESSION   DADMFL010000224 DADMFL010000000
VERSION     DADMFL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.232"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(2..298)
                     /locus_tag="JNZ27_004105"
     CDS             complement(2..298)
                     /locus_tag="JNZ27_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY8666193.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1050..1598
                     /locus_tag="JNZ27_004106"
     CDS             1050..1598
                     /locus_tag="JNZ27_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8666194.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1142 a          693 c          818 g         1027 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tggtttttct actgaaatga ttatgacttc aataaccctt gaatgaagtc
      541 tattcggttt tctaggagac ctgttaaaaa caggacaata tcaggtgaat gactatgcaa
      601 tttaatagta gatttccaag tatctaagca gttttacaca aattttatgc agtatcggta
      661 tagtctcttt ttagcataac ttattgaaat atatagagaa tctgtgaagt ctcttttcag
      721 ggaactatgc agggtgttag tcgttcctct ctcgactcct agaaaagtgc tatatacagg
      781 gctatataac gatatataga tgtttggtca catggtgtaa ctatcaagaa acttaacgat
      841 ttcctacgtg aaagttagaa agtgatggtt atagttaccc tcctaaaatc ttcatagctc
      901 cttcataacc agtcttaaaa ggacttcctt ttgagacact ttcaacgtaa cggtagaatg
      961 caccctcaag tagtctcaaa aggttgtttt tggattatga gacagctaaa cttatgagac
     1021 taacttttga gacttctaag gggaacatta tggcggtgat tggttattgc agggtatcaa
     1081 cacaagacca gactgtagac aaccagaaac tacagataga gaaggttcac aaggtcgata
     1141 aatggtttat tgatgaggct gtctcaggag ctacaaaggc caccagcaga gatggtttta
     1201 gaaacatgat gaactatgta aggtgtgacg atacgttagt tgtggtagct attgaccgtt
     1261 tagggcgtaa taccgttgat gtgctctcta acgtggaggc actacagact aaaggtgtga
     1321 gggttgtttc tctacgtgaa ggttttgact tattaacacc aatcggtaga gcaatgctta
     1381 ctatgatggc tggtttagct gaccttgaaa aagaccttat cagtgaacgt agaaaggctg
     1441 gtattgaacg tgctaaagct gaaggggtac atatggggag gcctgtaaaa gcttcttcag
     1501 aggctgtaca gacgcttatt tcacaaggta agacgaggtt gcagattcag gaagaattag
     1561 gaatctctag agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat
     1621 gtagctgata tttggctttt ttgcaaaggg ggtcacatag ctggtgtaac aggtacttag
     1681 cagctacaga catctgtcag atatttttga agattctcag agagggaatt tgaagagggg
     1741 ctactatgat tttataaaaa ttttcagagt ctgaaaagtt actgtacaga ttttatacaa
     1801 actaaaaagg actcccgtag gtgattcagt aatgaattac ttcatgaaga gtccctttac
     1861 aagtcgattt ttaatcgtgg ctacgacaat ccacccatac cagacctaac cgtaccccac
     1921 cttaccaagc cataccctac cccgccagac tataaggaag cgtagagaac cgatgaaggt
     1981 gtctgcacag ttgcatctta aacaaccatc acagtgatgt caaatagttc ttttaagtag
     2041 gaggggttcc cctatggtca catatcccct ctctcccgct tagtgtccct tgatagtatc
     2101 tagagagtat gcctgtaatg ttagagagta tgcctgtaat gtttctgtat caggtcagct
     2161 ataagaggtc tggatattac cttgtaagat tcttggtagt gattatgcag agggcttgat
     2221 aagagtgttt aacgggagga agagaaccat gaaaccgaag gtaggttcta tcttaacagt
     2281 gactgtaaga gatacctgaa aaaatacttt aacagaacta ccaagaagag agggaacgga
     2341 agagagggaa cgataagacc agaggtagtg acctggtaaa caggctgttt caaagtctct
     2401 taaaagtctg ttaagagggc taggtgaaca atcctaccat tttgtcaagt gttttgtctt
     2461 tagcaaaaag tgctaaactt aacaaaaagc actaaatgct aaagagacga ttaagaaata
     2521 tactaagagg ggcgcttcgc ttggggtcac tatgttcttt ctctcccgtt aagaggattt
     2581 acagggacat ttcaaagccc tgataaggta agcccgatga taacagaaac agagggcaaa
     2641 accacttatc aacactgtga acgaataacg taaagggctg gttaagaaag tcggtttagg
     2701 gattggtaac aataccaatt agaagggagc ttccctttct ggggtgtcgc tctatgggtg
     2761 aagttaacct ttgaagttct ctgtgaagaa actgaacagc tattatattg gctaattaca
     2821 accctttgaa agataagcat ttttagaagt tactgttaag gttgcagagt tgtctgtgaa
     2881 gagacttgtc agttctctat attgggtggt tacaactcat tgaaaaataa gagcttttag
     2941 aggttactgt cagagaggtg aatacttgat agatacagtc ttccggttct gtcaggctat
     3001 tttttaacag atagcacaaa atgctaaaga tgcccctatg ccatctcgta caggggggtt
     3061 ttgaagggag tttaacgggg gtctgtgtgg tcattatcct taaggtcaag cgtccatgtg
     3121 cgaccgaaca gtttaaagac aacaagcact ctgacgagga cgtaacgaag tagcagatct
     3181 gcaataagtc caatacctcc accagcccat acatcaggtg accccagctc aggagggaga
     3241 tttaccatta gttttccttg taaggcgctc tcaatcgcat tctgacacaa aaaagtacct
     3301 gaccttatga gttgacctga agaggtcatt taactcgtta cagagcgatt gaagaggtct
     3361 gtgtgcaatg caataaccat gtgaccaaat acatagccac gcctcatatt ttactctccc
     3421 cataaaccca ttataccaca gatgttacga aattgttaaa aatacttggt attagtcttt
     3481 gaaaatgcta gtattgtata tataagttaa cagaataata acatgttatg agggtcagaa
     3541 tatgaatgag agacgcagaa tttagagcga tgcttcaggc atcaagagaa cgcaacaagc
     3601 acaactccta cgcttacaca aacaacccca ccagctacga agtcccaacc tttagcaaat
     3661 ccgggtaatg actccaactt
//
LOCUS       DADMFL010000225         3603 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.233, whole genome shotgun sequence.
ACCESSION   DADMFL010000225 DADMFL010000000
VERSION     DADMFL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.233"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..107
                     /locus_tag="JNZ27_004107"
     CDS             <1..107
                     /locus_tag="JNZ27_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8666195.1"
                     /translation="ERESFNSAADHRLAELITGKLYDRIPKEIWKYVR"
     gene            146..736
                     /locus_tag="JNZ27_004108"
     CDS             146..736
                     /locus_tag="JNZ27_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY8666196.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            976..1236
                     /locus_tag="JNZ27_004109"
     CDS             976..1236
                     /locus_tag="JNZ27_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY8666197.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1460..1534
                     /locus_tag="JNZ27_004110"
     CDS             1460..1534
                     /locus_tag="JNZ27_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY8666198.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1527..2384
                     /gene="repA"
                     /locus_tag="JNZ27_004111"
     CDS             1527..2384
                     /gene="repA"
                     /locus_tag="JNZ27_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY8666199.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3084..3275
                     /locus_tag="JNZ27_004112"
     CDS             3084..3275
                     /locus_tag="JNZ27_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666200.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          955 a          908 c          904 g          836 t
ORIGIN      
        1 gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta atcaccggaa
       61 agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa ttcagcaata
      121 attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca ttgttctgat
      181 tattgcaacg ctacttatcc ggattatcct cagaagtgta aatcagcatt ctcctttgct
      241 gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac tgtccagcgg
      301 tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt ttatgaaagg
      361 actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac gggtcgcgga
      421 tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc agttattcag
      481 gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt tcagtattgt
      541 tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac gccgggatat
      601 tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc acgatgccag
      661 aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc agcagtcacc
      721 ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa acgggaaaaa
      781 gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt cacagtttaa
      841 atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc ttcgccacaa
      901 ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc cctgtttgag
      961 tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt gagtcaaaag
     1021 cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc cgctgttgca
     1081 agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct gaaagatctc
     1141 atggttgagt actgcgagag agaggggata acacaggctc agttcgttga gaaaatcatc
     1201 aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt ggcgtagcat
     1261 gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg tggcagggaa
     1321 ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa tcttctttaa
     1381 ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa caatcaagct
     1441 atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt gctcgctcct
     1501 tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat taccgccagg
     1561 taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt ccgttctgcg
     1621 ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc attcatgtgg
     1681 cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt cgccgggcca
     1741 ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac cgcgtccagt
     1801 gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct gccggaaaac
     1861 tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga ctgattacct
     1921 accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc acgttcacat
     1981 ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg cgccgcagtc
     2041 gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg ggtatggatg
     2101 aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac cagacagagc
     2161 ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg cagcgtcagg
     2221 atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg cgcttcactg
     2281 ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc atgattctgt
     2341 cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga cctcctcaga
     2401 ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc
     2461 agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg ccccccctgt
     2521 ttttgataca aaacacgccc cacagacggg gaattttgct tatccacatt aaactgcaag
     2581 ggacttcacc gtaaggttac aaccgttcac gtcataaagc gccagccgtc aaccctacag
     2641 ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta attacattca
     2701 tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc acctcccacc
     2761 gcaagcggga ggccccaacc gaagccggtt tagttacaac atgcaaacat aaacccctaa
     2821 aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac tgaaaagcgc
     2881 cgccgccccg cccgaagggc gggaacagag tcgcttttaa ttatgaatgt tgtaactaca
     2941 tcatcatcgc tgtcagtctt ctggctggac gtatcgagta cacgctcgta agcggccctc
     3001 acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc gggaccactc
     3061 cgaccgcgca cagaagctct atcatggctg aaaacgggta tggcttagca gggctgggga
     3121 tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc ggttttactc
     3181 cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc atcagaataa
     3241 tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt cgtttcaggg
     3301 atatcgttaa gacttgagcg ataggaatta aaaccccaaa aagattaaaa aaacaccaca
     3361 aaacggatgt ttcttcaaca ccacttttgc tacatatgaa cggaaccgac gattaaactg
     3421 gatggctctg attgattcag ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg
     3481 gacgcaactt cccctctgcg gctctcagcc gcaccaccgc atccgggcca gcggctcatg
     3541 catcaggacc tgctctgcca gacggtagcc ccgcttcagg cccgtaaaac gcatctgact
     3601 ccc
//
LOCUS       DADMFL010000226         3587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.234, whole genome shotgun sequence.
ACCESSION   DADMFL010000226 DADMFL010000000
VERSION     DADMFL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.234"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(623..2332)
                     /locus_tag="JNZ27_004113"
     CDS             complement(623..2332)
                     /locus_tag="JNZ27_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY8666201.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JNZ27_004114"
     CDS             complement(2764..3483)
                     /locus_tag="JNZ27_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY8666202.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1068 a          718 c          639 g         1162 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagtgtaag ggtcttc
//
LOCUS       DADMFL010000227         3526 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.235, whole genome shotgun sequence.
ACCESSION   DADMFL010000227 DADMFL010000000
VERSION     DADMFL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.235"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(63..1514)
                     /gene="cusS"
                     /locus_tag="JNZ27_004115"
     CDS             complement(63..1514)
                     /gene="cusS"
                     /locus_tag="JNZ27_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY8666203.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLQHLPRLFDRFYRVDPSRQRKGEGSGI
                     GLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1504..2187)
                     /gene="cusR"
                     /locus_tag="JNZ27_004116"
     CDS             complement(1504..2187)
                     /gene="cusR"
                     /locus_tag="JNZ27_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY8666204.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2344..>3526
                     /gene="cusC"
                     /locus_tag="JNZ27_004117"
     CDS             2344..>3526
                     /gene="cusC"
                     /locus_tag="JNZ27_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY8666205.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          865 a          913 c          923 g          825 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaagcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tgtaagtgct cgggcgcaat gggcgtaccg gggttttcga cggaaacttg cacctggtga
      301 tcgaccgtct gacagcgcac tacaattgtc tctctggttg gcgtataacg cagggcatta
      361 gaaagcaggt tgcttagcgc ccgacgcagc atcaacggat cgcccgcaac ctgacgtttg
      421 tcgccaacaa accgcaactc cacgccgcga tcttccgcta gcgcctcgaa aaaatcgaac
      481 actttgccga cttcatccgc caggttgagc attttctttt cggggattag ctggttgtta
      541 tcggcctgag cgagaaacag catatcgctg accattttcg ccattcgcgt cagctcttcg
      601 agattagagt agagcacatc ttccagctcc ttctggctgc gcgactggct gagggcgatt
      661 tccgtttgcg taatgagatt agtaattggt gtgcgaattt cgtgggcgat atccgctgag
      721 aaattggact ggcgagtaaa gacatcctca atacgctcga tcatatggtt gaacgacagt
      781 accagctgtt ccagctcaat cggcacggtc tgcgggtcaa ggcgaacgtc gagatctttc
      841 gaggtgatat tctggatttg acggctgacg ctgcggatcg gcgcgtgacc tttatgtacc
      901 gccaacagca cgataaagac gatcaggatg ctgattaccg atgcggtcat aataagttta
      961 ttcatcaaat catttatgta atgaagatga aaatcgatcg acagcgcgat gtagagcgta
     1021 taaatcggtt tgccgtccac caacgggcca accggcaagt taatcatccg ccagttgctg
     1081 tgttccatat gcccgtgacc gtggcctggc atcataatcg tcgggccgga aaggagatac
     1141 acctcgccac cccgcgcgtc tttatcgggt atggcatcac gtgcaaactc gcggatatca
     1201 ggcgcaccgg gggagtgata caccgttttg ccgtggctat ctgccaggga aatcaacacg
     1261 ttggaataac cactgacgat atcttccagc gtcattaagc gtcgggcttg cgtttcgtca
     1321 gggtgattta gtacccgttc aagggtggcg ctaatctctt ttaaatcatt aatgtcctgc
     1381 tcggcaaaat gcacttttac tgagtggatc atgatccatg caaaggcgaa aaacgccgcg
     1441 atggtggcca ggctgataaa aaaggtcagg cgggttgcca gcgaaaacgg gcgctgaaat
     1501 ggcttactga ccatccggca cctcaagcat gtaacccacg ccgcgcacgg tttgaatcag
     1561 cttcggctca aagtcgttgt cgattttgcc gcgtagccgc ttcaccgcca catcaatggc
     1621 gttagtgtcg ctgtcgaaat tcatgtccca tacctgcgag gcgataagcg agcggggcag
     1681 cacttcgccc tgatggcgga gaaaaaactc cagcagagta aattctttac tggtcagagt
     1741 gatgcgcgtg ccgctgcggg tgactttgcg gctgacgaga tcgaccatca gatcggcaac
     1801 ctgaaactga ctttcgataa tcaccgccgc cccgcgccgc aggagagtgc gcacccgcgc
     1861 cagcagttca gcaaaagcga acggtttcac caggtagtca tctgccccca actccagccc
     1921 cttgacgcga tgttcaatgg tgccaagcgc ggtaagcaac agaatcggca tccctttatt
     1981 ggcggagcgc aacatgcgca cgatatccca gccgttcacg tctggcagca taatatcgag
     2041 gattatcagg tcataatcac cggtcatcgc cagatggtag ccattcagcc cgttgtcggc
     2101 caaatcgacc acaaaaccgg cttcggttaa ccctttggtc aagtattctc cggttttctt
     2161 ttcatcttcg acaatcaaca gtttcatatt tcctccgcat gttgcccggg caattctaga
     2221 gtagcgggat cagatggcaa tcgcttattg gcaaaatgac aattttgtca ttttcctgtc
     2281 accggaaaat cagagcctgg cgagtaaagt tggcgacata aaatcaccag aaattatgag
     2341 cctatgtctc cttgtaaact tctgccattt tgtgtggccc ttgcgctaac tggttgttca
     2401 ctggcaccgg attatcagcg tccggtaatg cccgtaccgc agcagttctc actcagccag
     2461 aacggcctgg ttaacgcagc ggataactac cagaacgcgg gctggcgcac cttttttgtt
     2521 gataatcagg tgaagacgct gattagcgag gcgctggtga ataaccggga tttgcgcatg
     2581 gcgacgctga aagtgcagga ggcacgggcg caatatcgtc tgaccgatgc cgaccgctac
     2641 ccacagctca atggcgaggg cagcggcagc tggagcggca atcttaaagg cgattcagcc
     2701 acgacgcggg agttctcgac tggccttaac gccagctttg atctcgattt tttcggtcgc
     2761 ttaaagaaca tgagcgaagc cgagcgccaa aattatttag ccactgagga agcccagcgc
     2821 gcggtgcata ttttgctggt ttctaatgtc gcgcaaagct atttcaatca gcagctggcg
     2881 tatgcgcaat tgcaaatagc cgaagaaacg ctgcgtaatt atcagcagtc atatgcgttt
     2941 gtcgaaaaac aactgttgac cggtagcagc aatgttctgg cgctggaaca ggctcgcggg
     3001 gtgatagaaa gtacccgcag tgacatcgct aaacgtcagg gggaactggc gcaggcgaat
     3061 aatgcattgc aactgttatt gggaagctac ggcaagctac cgcaagcgca gacagtaaac
     3121 agcggcagcc tgcaaagcgt taaattaccg gcgggcttgt cgtcgcaaat cttattgcag
     3181 cgcccggata ttatggaagc tgaacacgcg ttaatggcgg ctaatgctaa tattggtgct
     3241 gcgcgtgcgg catttttccc gtctatcagc ctgaccagcg gaatatcaac cgccagcagc
     3301 gatctgtcgt cattatttaa cgctagcagc gggatgtgga attttattcc taaaattgag
     3361 atccccattt ttaatgccgg acgcaaccag gccaatctgg atatcgccga aattcgccag
     3421 caacagtcgg tggtgaatta tgaacagaaa atccagaacg cctttaaaga ggtggcagat
     3481 gcgctggcat tacgtcaaag cctggacgat caaatcagcg cccagc
//
LOCUS       DADMFL010000228         3497 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.236, whole genome shotgun sequence.
ACCESSION   DADMFL010000228 DADMFL010000000
VERSION     DADMFL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.236"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..266)
                     /locus_tag="JNZ27_004118"
     CDS             complement(<1..266)
                     /locus_tag="JNZ27_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY8666206.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLA"
     gene            complement(263..931)
                     /locus_tag="JNZ27_004119"
     CDS             complement(263..931)
                     /locus_tag="JNZ27_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8666207.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(995..1633)
                     /locus_tag="JNZ27_004120"
     CDS             complement(995..1633)
                     /locus_tag="JNZ27_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY8666208.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     ATAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(1646..3497)
                     /locus_tag="JNZ27_004121"
                     /pseudo
     CDS             complement(1646..3497)
                     /locus_tag="JNZ27_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
BASE COUNT          867 a          956 c          783 g          891 t
ORIGIN      
        1 gccagcaaca atacaaagag taacgcattc gaccagtgcc ataatctgac cgctttgctg
       61 taaagataaa ccttctcacc atgatcggtg gctgatttct tcacgccacg agcacgaagc
      121 aaggcgtgca gccccagcac caaccacatc ccaaccagca atactccggc aatgatcaac
      181 cacaccggcc aaaattcggg agtgaactgt ggcacatagt tcgctaactg gctctgaaac
      241 tgttcagcat gttgcgacgg gttcatacat tttccttttt aatcaaatgt tgaccgaacc
      301 ggcgatacag atgcggtttt ccggcaccag gtagctgata ttgatagtat ttattatcct
      361 gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa gatcaatgcg tgttccggac
      421 aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac
      481 atttatctgc cactttagtg acagggttaa ggtagcgaac ctggtacgga caagcgccaa
      541 tgcaataact gcagccgatg cactgcgatt tttctacccg cacgatcccc tgctcatcac
      601 gccacgatgc ccccgtcggg catacgtcga tgcacggagc atcttcgcag tgctgacatg
      661 actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta
      721 tcgataaacg gcttccctga gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa
      781 tattacagcc attgcaccgt gactcgtcat gaatcatggc ataccgaact tccttttctt
      841 gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa aaagattact gttcccatcc
      901 ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc ccctaatgca gtggctgcaa
      961 ctgtccaccc tatccatgac atctccctga aatttcaggg gttctgaaac tgctctacct
     1021 cgaaccattc gcataagcca taagccttgc cataggtcat aaatcgctcg ctaaacaagt
     1081 acaacaccgc cgtcgatgac tccacctgcc gtatatcggc atattcaggt gcaacatcca
     1141 tcgcggcggt cgcggcttca atctgcgctt cctggaagta atatggcgct tgcatcagca
     1201 tggcgacttt gtaaggacga ggatacgtct ggcactcaaa acgaacggcg tgtgcaatgg
     1261 cgcgacaaat gtcctgctcc acaacttgca aagacatcgc cgcgtagttt tcactcatgg
     1321 cctgcgttga gtaatagtaa tcatcctgac ttcccgtcat acaagcgata tccgcgtagt
     1381 ctccgttctg cttaatgctt tccagcaaaa ctgacagttc ctcttctgca accgaatagg
     1441 gtggcgagag aaacacggtg cgcgccacca gctgcccagc cgcagaatgc tggcgaatga
     1501 actcagccag gatttcagca ggttctggtt cggttaccgt atcaacgggg tgacgcatcc
     1561 cctgcaacca ttgccgcttc gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat
     1621 cctgatggtt catcttaccc attccttacg caggcagtag attgtgtgct gccagatcgg
     1681 cggcaatatc ttccagcccc agacgctgca atgtttcgcg ggttggacaa ccaagctgtg
     1741 ggtcccagcc catttctttg tagaacatcg tgagcgaagc atgcatatcg tcacgatcca
     1801 ttttgtcagt accttcagta aagaccggga tctgcggatc cttgtcgaaa acccaggaac
     1861 agataagatc gtgttcatta cgcatatctt tggtttgcat cggttttacc gtgtaggcac
     1921 gatgcaacgt aaagatacgc tctgcagcta aatctaattt ttcctgagtc atctcttcgc
     1981 cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc gccccggtaa ttacggcttt
     2041 tcagcggcga aacggtcatt ggccagaccc agttgcacag ggtgacggcg ttatgcaaac
     2101 agacccgcaa cagcgaccac tttgcatatt taatttttgc gtcattgatt ggcgtgtagt
     2161 ttttggtttc atcgtaagca tcttcagaac caaaaagttc tttcgccact tcacgttgca
     2221 gtttcaatgg caagccggaa ccaataaagt tgatatgggt atgcgtcatg cagtcacggt
     2281 tgaacataca gttaacaatg gaaccgacct gcgctgacgc ttcattggca tggtgaaccg
     2341 gatagccaaa cggcgaccag agtttatttt tcgcgtagcc ccagtactct ttacccaaat
     2401 tccagcgttc tgcgatggca tatgaaccat aagccaggtg actcagctca cccacacgat
     2461 gcgccagacg gtagtaaaaa tctttaatga agttaacgtc acccgcttcc agttgatccc
     2521 agcgaatttc tgcatactct tcagctggca gaacacgctt gaacacacct ttgctgtaac
     2581 aataagtaaa gtcgcgatgc aactgcccgt agttacacca taagccgtag tcatcgaaca
     2641 aattcagacc aaccaggtta ccaatcacac gaccatcgtc tttatcttca aaatctttcg
     2701 gcccgttcgg gaagatggtg gtatggacaa agtttgctac acaagtgtta ccacctgtgc
     2761 tgggcacgcc aaactctttc acccgaggaa tattcatttg ggtcatacaa cggatcgggc
     2821 aagagtggca gccgctcatt tttactgtgt atttctctgc cgccggtcct aagtcaaaaa
     2881 cggatttata ggtacgaaag ccgaccgtat tctgattgcc tggcggaatt tcacccgttt
     2941 caatcggacc accttcagcc gcgccccaaa acagcccttt acgtgctgtc cagcgtgact
     3001 tgggatctga atactctgcc cacgattgtg gcgtacttgg cacgacatgg ttgttattcg
     3061 caccaataag ttcagtcatc atgtaatcat tgagacgctt catctcctga cgatcggcaa
     3121 tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag gtttttcgaa cccattattg
     3181 cgccagttcc cgcaccgccg ctgtggttac ggctattcaa catgccagag agaggaataa
     3241 ggttttcccc agcctgacca atagccgcca cacaggtttc ccgactggtc aatcgacaaa
     3301 tttcttccgt cgttgcgcgc gtcccttttc cccataagaa atcggctttt tccaggctaa
     3361 ctttgtcatc tttaatcttc agccataccg gtgatttcgc cttcccttcg ataataatga
     3421 cgtcgtatcc agcgaatttc atttgcgctg caaaaaagcc acccatatgg gcatcgcgac
     3481 taaatttcct ttggtaa
//
LOCUS       DADMFL010000229         3414 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.237, whole genome shotgun sequence.
ACCESSION   DADMFL010000229 DADMFL010000000
VERSION     DADMFL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.237"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="JNZ27_004122"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="JNZ27_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY8666209.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="JNZ27_004123"
     CDS             complement(687..1160)
                     /locus_tag="JNZ27_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY8666210.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="JNZ27_004124"
     CDS             1492..2028
                     /locus_tag="JNZ27_004124"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HAY8666211.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="JNZ27_004125"
     CDS             2143..2469
                     /locus_tag="JNZ27_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8666212.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="JNZ27_004126"
     CDS             complement(2657..2896)
                     /locus_tag="JNZ27_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY8666213.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          943 a          756 c          722 g          993 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagggct gccg
//
LOCUS       DADMFL010000230         3330 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.238, whole genome shotgun sequence.
ACCESSION   DADMFL010000230 DADMFL010000000
VERSION     DADMFL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.238"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..299)
                     /locus_tag="JNZ27_004127"
     CDS             complement(<1..299)
                     /locus_tag="JNZ27_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666214.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQ"
     gene            complement(431..2272)
                     /locus_tag="JNZ27_004128"
     CDS             complement(431..2272)
                     /locus_tag="JNZ27_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8666215.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2272..>3330)
                     /locus_tag="JNZ27_004129"
     CDS             complement(2272..>3330)
                     /locus_tag="JNZ27_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8666216.1"
                     /translation="QRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVGPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
BASE COUNT          763 a          830 c          735 g         1002 t
ORIGIN      
        1 tgttgcgaac catacgcatc atatacagcc attgaatggc cttgctcagt tacaaatttg
       61 gcaatctgcc cgttgtacac aggatatccg gcagcattga taacaattgg ctgagaaaca
      121 gggacgtgag atccgtcttc attctctaca taaacctgaa tctggttttc aggatttacc
      181 gggtcagtgt caatttttcc gatataaatt ttgccattgg ctacggcttt aaaagaacga
      241 gccatagtga agagttgcga aggcatcgat acgatcacat tggctgtaat gtctgtcatt
      301 taatttgctc cagatacaat gaatcgccgc agcgtggcta cggtgaattt tgggcataaa
      361 aaaacccagc cgaagctggg tagttgcgtt ggttatctgt cagtagttat gtgcttattt
      421 aattatttat ctattgttct gctgtaattt ggcttgaaag tatagggcgt atcgcatttg
      481 cagcattatt taacgctctt tcataagctg ccgttcctgc tttggtgttt gccaaacgaa
      541 gaagcatatt ccttgcggct ttggactcat acaaacgcat cattgcacca aaaccagcct
      601 caagccccat tgatacacca agagtcgcag ttgcgccaat cgtccttatc ctgttggctt
      661 gcgattgccc cgtctgagtt actacatttg cggtgtctga ccttgctgtt tgctgtagaa
      721 cttcatgaag agcatcaagc tctttcatgt gctttccaga aaaaatagtg ttgtaaattt
      781 caccgcctga ctgagatttc agcttattaa cttcagtgat gaacttggct ggagagtcac
      841 cggccttttc cgctattttg ctgacgtaag ctgcacgcat agcatctttc cctttatcat
      901 ccagtgcgct ccagattcgt ttcacgtcag atggttttct gcttaataca acggtattta
      961 taagttcagg actggcttca ctgcttgcct tgttgagctt gttagcaatg tttttattaa
     1021 gcaccttatt ataaacgttt gcataatcgg aatttgcttt aaggtatttt gctgcgtctg
     1081 atgcaccgag gtttttagca actgcgttac gaaggtcttt tgacattgca ttctctacca
     1141 tattggtagc tgcttttgcc tggttgggga agaccatagc atctccctga acattagatc
     1201 taaatgctgt tctgtgctga cgcaagagat caaacgtaac atccaaatca gttgcagggt
     1261 ttgctaattc ttcacgtagg ttacgcaagg atgtaagcag gctttgattg gcagacgtcc
     1321 caagccgttc ctgtcttgcg atcgctgtat tcagagcatt catggtattt gtggtatcaa
     1381 ctgcggcatt acccatttta ttggtgacgt cattgataac agcgccagcg gcatccttcc
     1441 gcccccttaa cgtggtggta agagatttca ccacatcatc agggttgtac tcaccaaaac
     1501 ggtcaaaata attacttacc agcttactac gcgttgcata ttgctccgct cgctttgagc
     1561 ctgtcccgag caaagccccc tcggcatcct gagttaggcc gcgagtgaaa gcatttttcg
     1621 gcgggataac atcagatgtc attggtgtca cgcccatcga ttctgatgtg gcaattttct
     1681 tcgccacttc tggcgcaata tcacctttta tagccgttat tccacgccct attccctttg
     1741 ctgctgcgga aagaacaccc tgagcggcaa ggttaactcc ggcattttta gctgcatttt
     1801 gtgcgaaatc gcctttctga tttgcggcct ctgccagtga tccaatagcc atgcttcctg
     1861 ccgttccaac tcctggaact aaatacccac caattgtttc accggcttgc gcgtaggggt
     1921 ctgtcggtct gtctactgga cgataaacat catccaaaac cttgggtcca ccaagcccct
     1981 gactgattgc attaatcaga cttgcgccgc cctgcaatac gtcaaatggt atgtttacca
     2041 gaccacgacc agcctgttct gcaatttgcc ctgcactttg accaccagtg agccaatcgc
     2101 cagcttgttg catcaatgat ggttcttccc gtgttggtgc attattggcc tgattaactg
     2161 tttgttgctg aacagcctga ccagcaaaat actcatcaat ggcggtgcca atatcttcgg
     2221 tgctcgtacc atcaggaaag gtaaatgtct taccgtttgc agttactttc atcattccac
     2281 cgtaaattga atgcctgatt ttgaggtata tgatccaacc tgattccgtg gttctcctga
     2341 aggtgtcgaa tcttgtgctg gcgctgcgtc agtattcatt gacatatacc gcttaacggc
     2401 actccccaat gattcacctt ttttaacatc caaccccaat atctgaccgc cattacgcga
     2461 ttgtccaggg ttgccattcg cgctcatcca ctcggcttta aactcattaa actgcgcgtt
     2521 tcgtcgctca aggtttgcca ttgcatcaag ccatcttgcg accgtctcag ggttatccat
     2581 gtcagttggc gcaccctgtc gaacgatctc aacgtcttta tccgttgctg ggccgggagg
     2641 taggaattta agaacctgac tgttaacaag ggcattttgg cgaatgcgca aatcacgcaa
     2701 tgttgtatcg cttccggtaa gttttgcgaa catgttctgt gcgttaccga acaaacctgt
     2761 cgttggtttt tctgctctga actgttgagc aagcgcactc atagaattgg ctgagtttga
     2821 tgatgctgta gcattgttta cagccgtctc gatgcctttt tccatgttta ctgacagctt
     2881 aggtgcttcg ctaatcaact gctgagcctt ttcctgcgct tgctgcatct taaacccgaa
     2941 ctcttgctga tccagagcca agcgttgtgc tgcgatattg tgcccagtca ttgctgactg
     3001 ataggaaagg ttttgccctc tcgcctgaag tgcttctcca gcctgattgc tgcggattgt
     3061 ctctgccagc ctgcctcggt caatttcacg accagccatc ttgtcctgaa cattgaagta
     3121 atcaatcgga ccaagagcag ccattccaag gtgatcaaca aactcaccaa atcctgaagg
     3181 attctgctga tacatctgag caacgctgtt agggccaaca ccgacgcgcg ccagctcatt
     3241 ggcgttgttt tgcagccatg attgcattgc ttctggagac gaggccgcaa ggcgtgcgcc
     3301 agccgctaag gtgccgatag aattacgctg
//
LOCUS       DADMFL010000231         3327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.239, whole genome shotgun sequence.
ACCESSION   DADMFL010000231 DADMFL010000000
VERSION     DADMFL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.239"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..410
                     /locus_tag="JNZ27_004130"
                     /pseudo
     CDS             1..410
                     /locus_tag="JNZ27_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            994..1710
                     /locus_tag="JNZ27_004131"
     CDS             994..1710
                     /locus_tag="JNZ27_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666217.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2262..2666
                     /locus_tag="JNZ27_004132"
     CDS             2262..2666
                     /locus_tag="JNZ27_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666218.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2729..3028
                     /locus_tag="JNZ27_004133"
     CDS             2729..3028
                     /locus_tag="JNZ27_004133"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666219.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            3025..>3327
                     /locus_tag="JNZ27_004134"
     CDS             3025..>3327
                     /locus_tag="JNZ27_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666220.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT          932 a          685 c          772 g          938 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg cgcctacttg
      301 cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg ctgaccatta
      361 tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga aaactggatt
      421 gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa cgggcgagag
      481 catgacattg tctgttatta taatgagact gctgattcta cttattttta atttcaagaa
      541 aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata ttatcattct
      601 gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acatccctct catcgaaccg
      661 gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca cgttcgggcg
      721 gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc agtagattac
      781 cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt gaccaagttt
      841 tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat gataaatatg
      901 cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc aacttttatc
      961 tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt gcttagaaat
     1021 tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta ttcttatggc
     1081 tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg tttggatgat
     1141 gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac cggttggggg
     1201 attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga cttgagtttt
     1261 tttagactat tttttgctat taccctactg tttttccctg taatattcga acttttaatt
     1321 ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc attggttatt
     1381 tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc agatatccct
     1441 tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta tttttggctt
     1501 ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca gatgctttgt
     1561 tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt tttgtctagt
     1621 tcatttaaag atgacagtaa cagattctta atatttaata cagaacagtc gggattttat
     1681 gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg aattaaatga
     1741 atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca aaccttaggg
     1801 cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt gctcggaaca
     1861 gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg ccaaacatca
     1921 caaatcacca gccggactga gtgatgccaa gcattgcacc aataccccgt gatgaacgat
     1981 gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg cgattcaccg
     2041 tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac tgaacaactc
     2101 tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag cttccacttc
     2161 cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag gacatgaggt
     2221 gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact gcgctgatag
     2281 ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact ttactggcta
     2341 tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga ccatatgggg
     2401 tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa tgtaacagtg
     2461 actactatta ctctgataaa ccaccgggag tacagatact cggaacccgt gcagggtgtc
     2521 gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga ggggttgtgt
     2581 gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg aaacgtcctg
     2641 tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg cggctaagtg
     2701 agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca gcaaaaaaac
     2761 acgtaaacag cattcgcctg aattttgcag tgaagccctg aagcttgctg aacgcatcgg
     2821 tgttgctgcc gcagcccgtg aactcagcct gtatgaatct cagctctatg cgtggcgcag
     2881 caaactgcag caacagatga cctcgtctga gcgtgaaagt gaactggccg ctgaaaatgc
     2941 ccgcctgaag cggcaactgg cggagcaggc agaggaactg gctatcctcc aaaaggccgc
     3001 gacatacttt gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca tcaggctgag
     3061 ttcagcatca aggcaatgtg ccgcgtgctc cgggtggccc gcagcggctg gtatacgtgg
     3121 tgtcagcggc ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt
     3181 gtcctcgctg cggcctttac ccggtcaaaa cagcgttacg gtgcaccacg cctgacggat
     3241 gaactgcgtg ctcagggtta ccactttaac gtaaaaaccg tggcggcaag cctgcgccgt
     3301 caggggctga gggcaaaggc ctcccgg
//
LOCUS       DADMFL010000232         3210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.240, whole genome shotgun sequence.
ACCESSION   DADMFL010000232 DADMFL010000000
VERSION     DADMFL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.240"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..298)
                     /locus_tag="JNZ27_004135"
     CDS             complement(<1..298)
                     /locus_tag="JNZ27_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8666221.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIP"
     gene            545..1306
                     /locus_tag="JNZ27_004136"
     CDS             545..1306
                     /locus_tag="JNZ27_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8666222.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1413..1925)
                     /locus_tag="JNZ27_004137"
     CDS             complement(1413..1925)
                     /locus_tag="JNZ27_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666223.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRIQAYIYAE"
     gene            complement(2064..>3210)
                     /locus_tag="JNZ27_004138"
     CDS             complement(2064..>3210)
                     /locus_tag="JNZ27_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8666224.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTRLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT          967 a          719 c          574 g          950 t
ORIGIN      
        1 tgggtatcgt aagaatcaca ttttcttcaa caccaatatt tttaaaactt ttgcgtaaca
       61 gggatatata ttggttcaat gtgttgtttg acagatcaaa tgaaaatctc tccaaaacgc
      121 tatcaatgat tgagttcctg tcaaccgctc caccattact ttccagtaac actgccaaaa
      181 tacaccccgc cgtagctggc aaatttatct catcctgggt atttacattc gagagcgagc
      241 atttgttcgg atcaaaaata atcgcgtcat tgataatgaa aaaacatctc cgctccatgc
      301 aggcaacaac acaaaataag attttgtcat tttagcctat tcctggtgga actgacacaa
      361 gaaactaaaa tacaaatata accctgaaaa agtcatataa aacttatatc cacagaataa
      421 aaaaaccaaa catgtcagtt ttatcaatat aaagtatcgc aaggcgaaaa gtgatgcaag
      481 aatactccta agtaaatgaa agcgcaccac atgaaattat caacacatgt aatttcatca
      541 ttaggtgcta tcgaaaataa ctcaggagct acttaagatg aataatgtct tactcatgag
      601 acccgatgac tcagaattta tgatacctga tgactggtca gggctaaacg caaaaggcat
      661 tacatacgaa caagataata gagtgacaga gtcttttctg ccttttaaat ctttttattg
      721 ttcaccggtg tgcacgacag ttgaactcat ctcacaaaat aattgttttg ctatgggtat
      781 ccgctacctt ctggaaaaga aaagagactt aaggctaaat cactttaaag ccttctctca
      841 taaatgcgag acaaaacctg acgtgttaat tatgaccggg tgcaccgtcg ctgatactta
      901 tagtatattc tcacgcattg ttgaagaaaa tataatgaaa acgatgacac ctatcgtcat
      961 cattactggg catcactcag aagatctttg ttctctggca tcgaaactgg gaggagttaa
     1021 aataatatca ccgacagaaa agattgcaaa aatagaaaac gctatcaatg atgcttttaa
     1081 cagcaagcct aaaaaaataa gatttttaac agatatactc tctcctcaac aaattgagac
     1141 catgaaaatg gttgcgcagg gtagatccgt taatgagatt tcagccgcaa tgaatattag
     1201 catcaagacc gtgtatgcac ataaagttcg tgctatcaat aagctgggaa ttaagaaaaa
     1261 agtttatgaa gcgctttttt ataaagcgct aattcagttc aactgatgta aaaatcaccc
     1321 tcgcttaata aataatatat tctatgtctt tttttataag ttaaaaaagg cgggatatca
     1381 aatcccgcca tgaaattaac taatcatctg tattattctg cgtagatata agcctggata
     1441 cggatggtgt aagagtctgg ctcaacaacc tgttctttca tatgggaaac gacgcgatag
     1501 gcaccacctg ttccagtatt tttcagcatc atataacgct taccgccaat ctcttcggag
     1561 gcagctgaag gaggatcctg ctcaagcgtt gtcagggaaa ctttcagttt atggtccggg
     1621 ttattgtcac cagaaattac tgcatccgct gaagtactgc tctccgcata aatatcatcc
     1681 gggaaagtta cagcatagtc gtggctctgc ccatcagatg cgccaacaat acctgttgcc
     1741 aaaggcgtcc atgcagttaa cgtaccgcta tatacccctt tcactggttg aatggtatta
     1801 ttaacaacga taggcttggt caaagtaaga ttagctgacg cttcagtacc ctggcttact
     1861 aaagttcccg cctgagaagc aaaagctgtc cccgccagca tgattgctaa caatgttttc
     1921 ttcattatat tttctccatg attttcattt acactgcaaa atgcagtttc tttctttgca
     1981 tttacgtgct atcgcgaaaa tgcttccacc aaataatgga aacatcttca caaaaataaa
     2041 ttatgagttt cttaatttag aaactataaa catgtcgccg ctatctggta taaccctgat
     2101 gcacttttct tcgcaggaag ttggtatttt gcgatacagg attgatcttt agaataaccc
     2161 catttcacat taaactcgcc ttccgcactc aagccagaaa cataagcaat accatcttca
     2221 cctacaatgc cggccatatt ttcagtgtct ttgctataac taatcacagc gccgaagggg
     2281 acgggtttac cctctttctg ttttaaggtt actaatcctt tatccccgct cttcactgcc
     2341 aatgtcgcag gcacaacggc tccagctgtc ggtacaacca attgactagt ttgtggtaag
     2401 tcgtaattct tacctatcgt tttttcatcc agaataacat cgtctcttcg gtatggctgc
     2461 actggcagta ttgcataccc acgaaaatca gtactcagtc ccggccagcc attaacgtta
     2521 acccccgttg ctccgggtgc ctctataagg gctaatgtcc catccacatt ttgaccaaat
     2581 gtaagtccat cccttgtaat aagtatccca ccgcttaaat ttgcacctag ctgtttcatg
     2641 ttttcgctgg agctgtaatg gctactaaac tgaccatatg atccattttt gccaacttgc
     2701 acaatgttca tcgtgctctt cggttgtgac tgatgagcgt tagttatatt tgtcgctaca
     2761 tcccaccata caccaccact attctgacct gataatgaaa cctgatagtt attaccctgc
     2821 tctcccgaac gactcatctg tgaagacgca taaatttgat tttcgtcacc cagcgataat
     2881 ggcactgaaa tacccacgct taatttccga tctactttcc atttatgcga tgtcagcaag
     2941 tcgttttttg cccagttaat ggagaatgtt atgcctctcc ataccgtact ataactcagt
     3001 ctggtagacc gcgtgtttcc tgtgccccgg gaagaattat acctcgtggt attgaagttc
     3061 aaattcccga tattaccaag gggctgggat aaggatatcg tagagccatc gtgactattc
     3121 ttatctcttt ttctgttccg ttcatcctgt gcacttactg cttcagtcag tgtggcataa
     3181 cctgatgcat aacggttcgt ggttatagac
//
LOCUS       DADMFL010000233         3178 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.241, whole genome shotgun sequence.
ACCESSION   DADMFL010000233 DADMFL010000000
VERSION     DADMFL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.241"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            848..1042
                     /locus_tag="JNZ27_004139"
     CDS             848..1042
                     /locus_tag="JNZ27_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8666225.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESETGT"
     gene            1229..1663
                     /locus_tag="JNZ27_004140"
                     /pseudo
     CDS             1229..1663
                     /locus_tag="JNZ27_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
     gene            1688..1822
                     /locus_tag="JNZ27_004141"
     CDS             1688..1822
                     /locus_tag="JNZ27_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8666226.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2067..3176)
                     /gene="fepE"
                     /locus_tag="JNZ27_004142"
     CDS             complement(2067..3176)
                     /gene="fepE"
                     /locus_tag="JNZ27_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY8666227.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          942 a          779 c          660 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcacc cctaatataa gatgatgttt ttttatgaaa
      421 atagtcagcc ccccccccct aaaaagcggt gttggcgcag ctacaacacc gcgccgacac
      481 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
      541 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
      601 cagactcgcg tttttccgga gtatcagcgg aattattgat taagaaacac tcgacgcaga
      661 gcgaaatatt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
      721 tgccatgata cgtcctaaag aaacaggaca ggatgtgcaa ttgtaatacc gtcgcccgca
      781 acgtcttaca attaccacct gctcagggtc ccccccgaca cccggaactg acggagaaca
      841 acggaaggtg aacaaacaac aacaaactgc gctgaatatg gcgcgatgta tcaaaagcca
      901 gagcctgaca ctgcttgaaa aactggatgc tctggatgcc gacgaacagg cggccatgtg
      961 caaacgactg cacgaactcg cagaagaact ccagaacagc atccagattc gctttgaggc
     1021 cgaaagtgaa acaggaacat aacgaagctg acggaggcgg tcacggcttt cccgtgggcg
     1081 ttttcgtcag cagacaagcc cgtcagggcg cgtcaggggg ttttagcggg tgtcggggcg
     1141 cagccatgac ccagtcacgt agcgatagcg gagtatatac tggcttaatc atgcggcggc
     1201 agagcagatg gtgagagaac agcgacatat gaggtgtgaa atgctgcaca gatacgtaag
     1261 gagaaaatgc agcttccggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1321 cgttcggctg cggcgagcgg tgtctgctca cgcaaagacg gatatacggt tatccacaga
     1381 atcaggggat aacaccagaa aaaacatgtg agcaaaaaag aggccccgaa aaaaggccgc
     1441 gccggtggcg tttttctata ggctccgccc ccctgacaag catcacaaaa atcgacgctc
     1501 aagtcagagg tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggtggc
     1561 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1621 ccgcgttcgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1741 tcttgagtcc aacccggtaa gacacgactt aacgccattg gcagcggcca ctggtaaaca
     1801 ggatgtgcga gagatgtatt gagagttctt gaagtggcag ccttactgag gctacactag
     1861 aaggacagat ttggtgacat gtctcgcaca agactgttac cacggttcag aagttcccca
     1921 acttactgaa ccttcgaaaa accacctccc caggtggttt tttcgttttc tagagtagaa
     1981 gactacggct gtaagggaat gatctcaaga agatcctttg aactttttat aaaagttgct
     2041 tataaaatga tgcatcagca caacttttac tcaatcacac tcatctttct ggcctccatg
     2101 gcatggcgta aaagtatata gccacatgca agcatccctc caatgacaga agaaagaatt
     2161 acaattatgg cattacttgg cccatccttt ctgaccggta acgttgggct caattgatac
     2221 tgaaatggca taaaatcaac atcccccact ttcgccacaa ccaattgatt cacaacatat
     2281 tgacgatttc gcaactcccc actcaattcc gaaacatcct tgattgattt tttgatgttc
     2341 aatttagttg ctataccatc agaaccgaga gccacaggaa aatctgggtc atccttcata
     2401 atctgaccat tgctgtatac aggttttttt attccagccg cattagcaac ctccagtgaa
     2461 taattgaggc gtcgaatatc tgcattcagc tggtttctaa tttttatctc atccagtgca
     2521 agaatttctt tttcaacatt agttttaact tctagtttat ttcttatatc ttccatcaaa
     2581 tcccttacaa caagggaaga aacataatta atatacccag acaacacatc gtgagcttct
     2641 ttacttgttg gagcagtaaa actcagagtc catgatacat acaatgatga cttgtcgttt
     2701 ttcttactgg cattatcatc aatcgacttc atatttttac tcaactcaac aattgcacga
     2761 tgcagttcga gagcgctaat gttattcttt ttaataatct ccataacata aggagatgat
     2821 ctcatatact cattcaacga attaacggac tgaaatttct taataaacag agcaaatgcg
     2881 ctatcacgat tgatatcaaa atccactccc agaacatgaa gcttggaaag ctccttattt
     2941 aattcactcc attgaacaga ctcagcagga gtaaccacag cagaactagt ccatttttgt
     3001 ggcaaaacaa aagaaacaag aaagccaata caagcaaaaa atagaatcac cccaacaatt
     3061 tttatttttg ctgcccataa aacgctaatc aagccaaaaa ggtcaatttc ttcattacca
     3121 ttcggcaaca tgttgtaatt agaaaaatgt tcgtcagaga cctttgagat tttcattg
//
LOCUS       DADMFL010000234         3144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.242, whole genome shotgun sequence.
ACCESSION   DADMFL010000234 DADMFL010000000
VERSION     DADMFL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.242"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNZ27_004143"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNZ27_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY8666228.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JNZ27_004144"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JNZ27_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNZ27_004145"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNZ27_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY8666229.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNZ27_004146"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNZ27_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY8666230.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3144)
                     /locus_tag="JNZ27_004147"
                     /pseudo
     CDS             complement(2194..3144)
                     /locus_tag="JNZ27_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          761 c          764 g          838 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc tctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccaactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta gcgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gggg
//
LOCUS       DADMFL010000235         3125 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.243, whole genome shotgun sequence.
ACCESSION   DADMFL010000235 DADMFL010000000
VERSION     DADMFL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.243"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..88
                     /gene="ldrD"
                     /locus_tag="JNZ27_004148"
     CDS             <1..88
                     /gene="ldrD"
                     /locus_tag="JNZ27_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAY8666231.1"
                     /translation="MAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(175..1854)
                     /gene="bcsG"
                     /locus_tag="JNZ27_004149"
     CDS             complement(175..1854)
                     /gene="bcsG"
                     /locus_tag="JNZ27_004149"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY8666232.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGAAPVVGDMPAQTAPPTTANLNAWLNNVYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1851..2042)
                     /gene="bcsF"
                     /locus_tag="JNZ27_004150"
     CDS             complement(1851..2042)
                     /gene="bcsF"
                     /locus_tag="JNZ27_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY8666233.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2039..>3125)
                     /gene="bcsE"
                     /locus_tag="JNZ27_004151"
     CDS             complement(2039..>3125)
                     /gene="bcsE"
                     /locus_tag="JNZ27_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8666234.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          774 a          804 c          828 g          719 t
ORIGIN      
        1 catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat
       61 cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg
      121 caatttgcgc ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg
      181 cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac ggtttatctt ggtattgaat
      241 aactactgca tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt
      301 tttccagtca acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc
      361 ggagatagcc aggaagctgc tcggttggtc aatgacaatc ggtgccccct gatgtggtgc
      421 cttcatgccg aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc
      481 acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac
      541 gaccaccatc actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc
      601 aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt
      661 gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc
      721 ggtaacgtcc agccagcggt taagcacggc ggtatcgtca taaaccggcg aaccatcaaa
      781 gcccagcaaa ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt
      841 ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg
      901 ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata
      961 cagattagtg tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc
     1021 actgtaggag gtggcggagt taaagttctt gaactcaata tcgaaatgcg accacagtgg
     1081 atgcgacatc aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac
     1141 cagtagttca aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt
     1201 cgcctccgcg ttatagacat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc
     1261 agtttgtgcg ggcatatcac ccactaccgg tgcggcaccc gtcgccgcaa cggttgccgc
     1321 tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa
     1381 acttggtccc gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt
     1441 aatgcgaatc cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat
     1501 catctgccag ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc
     1561 cacctgcgaa ccctggctca ttatgctttc cgggccaagc aaccaggtgt catgccagaa
     1621 caaagcaaag ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtaacgcgg
     1681 aatgggcatc agcagaaacg cggcaaacac caaattgagg agcgggtgga agttaagata
     1741 tcccgcccac aacaggccaa acttaaccag aaaatagaag ttccagccgg aaaggccgcg
     1801 ccagtattgc cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg
     1861 gttgccctgg ctttttccgt gcggcgtaac gtcccggccg gtttaacata acgaggttta
     1921 gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc
     1981 agcgggaaaa atatcagtgc gcaaacgata ataatttcaa tgatatcgct gatggtcatc
     2041 atgatgagcg ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt
     2101 ggcgaccatc gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca
     2161 ttccccattg ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat
     2221 cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat
     2281 tcaacgcggt atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac
     2341 cgccaatggt catgatatcg ccggtacggt tagggcgaca tagcgtcagg gcttgctcaa
     2401 cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggcaaga
     2461 gagggttatt catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac
     2521 gcagtttgag cggctgggtc attgacagca aggtagtgat atcttccggt acatagcgac
     2581 taaacttctg cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc
     2641 acggaataac catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc
     2701 tagcggtatt ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat
     2761 gaatgctgcg ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg
     2821 ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt
     2881 gttccgatag cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt
     2941 cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt
     3001 gctgaacgct aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat
     3061 ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt
     3121 cctca
//
LOCUS       DADMFL010000236         3040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.244, whole genome shotgun sequence.
ACCESSION   DADMFL010000236 DADMFL010000000
VERSION     DADMFL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.244"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            54..1085
                     /gene="ynaI"
                     /locus_tag="JNZ27_004152"
     CDS             54..1085
                     /gene="ynaI"
                     /locus_tag="JNZ27_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY8666235.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1136..2749)
                     /locus_tag="JNZ27_004153"
     CDS             complement(1136..2749)
                     /locus_tag="JNZ27_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8666236.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          793 a          707 c          670 g          870 t
ORIGIN      
        1 ccgccagggg tttccggcat gacgacaatt atctggagga gcctgataaa acaatgatcg
       61 ctgaactgtt tacaaataat gcgcttaatc tggtcattat tttcggtagc tgcgcagcat
      121 tgattctgat gagcttttgg tttcgccgtg gaaatcgtaa aagaaaagga tttttattcc
      181 atgcggtgca atttttaatc tacaccataa ttatcagtgc tgttggtagc atcattaatt
      241 atgttataga aaactataaa ctcaaattta tcaccccagg cgttatcgat tttatctgta
      301 cgtccctgat tgcggttatt ctgacgatta agctatttct gctgattaat cagtttgaaa
      361 aacagcagat taaaaaaggt cgcgatatca ccagtgcgcg gattatgtcg cgcattatca
      421 aaatcaccat tattgtagtg cttgttctgc tttatggcga acatttcggc atgagccttt
      481 ctggcttgct gacctttggt ggtattggtg gtctggctgt cggtatggcc ggtaaagata
      541 ttctgagtaa tttcttttcc gggatcatgc tctatttcga ccgtcctttc agtattggcg
      601 actggatccg ttcaccggac agaaatatcg aaggtacagt agcggaaatt ggctggcgaa
      661 ttaccaaaat tacgaccttt gataatcgtc cattgtacgt accgaactcg ctgttttcgt
      721 cgatcagcgt agaaaaccca ggacgaatga ccaaccgccg cattaccacg accattggtt
      781 tacgttatga ggatgcggca aaagtgggcg ttattgtcga agccgtacgt gagatgctga
      841 aaaatcaccc ggccatcgac cagcgacaaa ccttactggt ttatttcaac cagtttgctg
      901 actcgtcatt gaatattatg gtttattgct ttaccaaaac cacggtatgg gctgagtggc
      961 ttgctgcaca gcaagacgtt tatttgaaga ttatcgatat tgtacagtca cacggcgcgg
     1021 actttgcctt ccccagccag acgctgtata tggataacat tacaccgccg gaacagggtc
     1081 gctaaatcag acatcactgg cgttatccac aagggtaacg ccagttccca tcacatcaat
     1141 gcttcacaat atacatagtc cgactgtacg ctacatcttc agggttatta atgggataac
     1201 ctttcagcca cggtttgatt aatcgtccat tggtatattg ataaattggt gcaatcggtg
     1261 cttgctccat gaggattttt tctgccgcgt tgtaatcggc attacgcgct ttaacggtat
     1321 tttccgtcga tgcctgggcc agaactttgt cgtatgccgg attgttaaag cgtgaaatat
     1381 ttcctgaatg cattgacgtt aataatgtca ggaaagtgga gggttcatta taatccccca
     1441 cccatgaggc gcggataaca tcaaaattgc cggtgttacg gctatcgata taggttttcc
     1501 attcctgatt ttgcaattta acatctacgc caaggttctt tttccacatc gatgctacag
     1561 caatcgcaat tttttgatgg ttttctgaag tgttatacaa aagcgtcagc ttcagcggtt
     1621 tttgcggacc ataaccagct gcgctcaaca aagtttttgc ctgcgcattc agttcttcct
     1681 gactcatttg ttcaaacggc gaaggttccg gcgtaaatcc ggcggtaaca tctggtataa
     1741 aatgccacgc tggcttttcg cccgtcccca acactttttc ggtcatcagg cggcgatcta
     1801 tcgtcatact taatgccagg cgaacgcgct gatctgccgt cggccctttt tgcgtgttaa
     1861 aggcataata ataggtcccg agctgcggtg gcgtataaac ctgccccgga atatccttca
     1921 acagcttctg atacatattt ttcgggaagg attcggtgat atcaatatcc cccgcgaggt
     1981 aacgcttagt ggctgcggat tcctgattaa ttggcaggaa ggtcactttt tgcagcaccg
     2041 ttttggcgtt atcccaataa tgggtattcg gtaccacgac cagtttttca ttgactacgc
     2101 gctctttaag aacataagcg ccattgccga tcagatttcc gggtttcgtc cactctttac
     2161 cgctttctac gttggctttt tgcaccggga agaaggcaaa gttagcggtt aaattcacaa
     2221 accacggcaa cggtttatca agctgaattt tcaaagtatg ggcatcaact gcggtgacgc
     2281 caagctgatc aggcgtagct ttaccatcaa taatcgcctg tgcgttgttg attcccgcca
     2341 gcgcggcaaa ccatgcaaat ggcgacaatg tttttgggtc caccagacgt tgccagctgt
     2401 agacaaaatc ttgcgccgtt accggtgtgc catccgccca ttttgcgtta tcgcgcaggg
     2461 taaaagtcca gatacggttg tcattacttt tccattgagt cgcgacgccg gggacaatct
     2521 cccctttttc attctgattc accagacctt caaacagatc gcgaatgacc tgaatctctg
     2581 gcaggcccac ggctttagcg ggatccagcg acgcaggctc atctttaatg tggcgcacca
     2641 gctcctgctt ctctgccagt actgtgccgc tcggaacttc tgcagcatac gaaagagaaa
     2701 tgctgctgac caacagcgca caacacgtaa ctgaaacaga gtgcttcata agtttccctc
     2761 taaccgacat taaaattgat gcgtaattat ttgtttcgcc ttaaagaaat gcaaacaact
     2821 tccatcaaaa aagtgacaaa ggaagcatca aatgttttaa gtatcaatat atcaaatcag
     2881 atttagtgta aataatagac gccaaaatgc ggcaaagcac atagaacacc gaacactacg
     2941 ttcgggcttc gcacgttcaa accagttttc ggtatcataa tcagcatctg tcgccattgt
     3001 acgaagaata aaaaataacc aaaacgttga aaggaactga
//
LOCUS       DADMFL010000237         2982 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.245, whole genome shotgun sequence.
ACCESSION   DADMFL010000237 DADMFL010000000
VERSION     DADMFL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.245"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..276)
                     /gene="ssb"
                     /locus_tag="JNZ27_004154"
     CDS             complement(<1..276)
                     /gene="ssb"
                     /locus_tag="JNZ27_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY8666237.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQ"
     gene            complement(277..984)
                     /locus_tag="JNZ27_004155"
     CDS             complement(277..984)
                     /locus_tag="JNZ27_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY8666238.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(1239..1391)
                     /locus_tag="JNZ27_004156"
     CDS             complement(1239..1391)
                     /locus_tag="JNZ27_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY8666239.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(1376..1507)
                     /locus_tag="JNZ27_004157"
     CDS             complement(1376..1507)
                     /locus_tag="JNZ27_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY8666240.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(1532..2500)
                     /locus_tag="JNZ27_004158"
     CDS             complement(1532..2500)
                     /locus_tag="JNZ27_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HAY8666241.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(2668..2910)
                     /locus_tag="JNZ27_004159"
     CDS             complement(2668..2910)
                     /locus_tag="JNZ27_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666242.1"
                     /translation="MRVKTMGASPLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
BASE COUNT          608 a          774 c          572 g         1028 t
ORIGIN      
        1 ctggtctttc cactttctgg ttctcagcat tccctcgaaa taaatctgat cacctttttt
       61 cacatactgc cccacgacct cagccagttt cccggataca gcaacacgat gccattcagt
      121 caattccttt tgctcgccag tatttttatc tcgccattgt tctgacgtgg ctattgtcag
      181 gttagcgaac gctgttcctg atggtgagta tcgaacttcc gggtcttgtc ctacccgacc
      241 aaggataatc accttattta cgcctctgct tgccatttat gccgcctgtt ttagttcgtt
      301 aactctgatg ttcattacct gaacgcattt agcctgcgct tcctcgttgc cagccattaa
      361 ttgccagtca cgctgataac gctcgatgag ttttttcttg tcagtttctg ttgacgcata
      421 atcgctgaag tctttcagga tttgttcgca gtcaaccgat ggagatttct ggttggtatt
      481 ttctggtgat ggtttgttat ctgatgctgg gattgcccat cccggcagcg atggagggag
      541 ccagtaaaat cctgttccat ccttcagttt tgccctgtgc cacccatgct ttttatcgag
      601 agatgtttgt gcgaaacctt cctcaaggtt atacagatac cgaccgattc cccactgaac
      661 ggcagcacgc ttcattgcac ctgaacgacc acctttgacg gcttctacct gcgtgttttc
      721 agcagcatcc catttggtta cccattcgga atcaatcctg attgatatgc cgcattcaac
      781 gcctccgttg ttgggaatat cgcggtattc attgcgccat cctgctttgc cgcaaacatc
      841 gtccaggcgt ttcatgattg cccggttcgt gacataagcc agcaccatag cccacacttt
      901 gccatcgcgt gttttaccgc tttgctgtat tcgccattcg atatcttcag ggctgaatgg
      961 ctcatcgaat ttattcaaat ccataaatca cctcagaatg gacatggccc aagaaaataa
     1021 cgctgattta atacttcgac tcgggacaaa ttaaggcata cccgcattcc ttcgcggtcg
     1081 ccattatggc gataccagag agctttctgg gtgtacatgc gtctctgtaa cttgctctcc
     1141 ttcactgtgg ttgcaagtga catgaatatc tccttcgttt ccgattaatt ctttcatctg
     1201 acgaatgaat tcttcgtctg accagttatc tgtaaaactc atttcctgcg ataccacgga
     1261 aggttgatag ctgacttcat cgctttattt gcttcaagcc acattttgga atcaccaata
     1321 aatctggcta ttactgcttt gttctgtgca gcacgaagca tctggtgatt gatggctatt
     1381 tcattgcgca taataagacc tcaactcttt tccatccgtc acgtaattta cgggtgattc
     1441 gttcaagtaa agattcattt aattggaagg cacccatgcg agcgcctccc gcgattgcgt
     1501 aaatcatggg tggttcctta tgttggtttt attagtaggt tatttttgtt gcgaatactt
     1561 cgccttttac gatggctgtt atgatatttt tagcaacatc ttctgatgcg ccaaccttga
     1621 taaggtcagc aagtattttg ttatttactt cttttcggtg agctttatcc tttgctctac
     1681 gctcttcttc gtccttgatt cttttttctt ctgctattct ggcttgctct tttgcttcag
     1741 cttcgcgccg gattcgttca gcctcctcct gtgcttttcg gcgttctgct tcaattgccg
     1801 cctgcttttc tctttcagct cgttctgctg cctcttttgc ttcgcgctgt gctcgttgct
     1861 cggcttcaat gcgttcacgc tctgcacgtt ccgctgcggc cttagcttct gcttctcgcc
     1921 ttgctgctgc ttcaatttcg gcttttgcct ttgcttcggc ttctgctctg gctttctctt
     1981 cagcttctct ttttaagcgt tcttcatgct ctcgcttttc ctgctccgct ttgagtcttg
     2041 cctcttctct ttggcggtca aattcgcgat ccatcaaaat cgctatttca tggtcagact
     2101 caatttgctt tgcgagagct tcagctgctg ccttagcttc ttcttcggct ttaatccgcg
     2161 cctgttcctc ctcataatca gtaagaggct ggcgcgcctt ggctttcagc tcatcaagtc
     2221 gatcatgcac tgttttgcgg ttggcatcaa ttagctttgg aatttccttc agttcagcaa
     2281 caaggtcttt gccaagacca tcgagatatg ttttcgtctg cgcaacttta tacgccagag
     2341 aagcgatctc ctttctgccc tttgccgttg tgatatcagg cacaaaggac ataacttcac
     2401 gttcaacctt ttgaaggatt tcttcaatct ggtcggcaga ctgaaataca gtcattgcat
     2461 ttgctttttc aataacaact aaatctgtta cttcactcat atttcccccg tcaaaaaaat
     2521 tgccctcaca ctggagagca aagaagattt ccaataatca gaacaagtcg gctcctgttt
     2581 agttacgagc gacattgctc cgtgtattca ctcgttggaa tgaatacaca gtgcagtgtt
     2641 tattctgttg cttattccaa agcaaattta accatcaaca aactcttctg gtaatttatc
     2701 aaccagttga tgacttatta tcagccattt gccatccttc gtttcgtatg catatttctg
     2761 gtcttttatc atcatgtgtt cggctactgc cctcactgcc tgatcggtga catcttctct
     2821 ctttcctacc cacattcctt tttcagtgtt taatgttcct tgaaaaatac gaccacttaa
     2881 tgggcttgcg cccatagttt ttactctcat gtatcagtcc tcaaataagt ggtttgctgc
     2941 gaaagtaaat acgcttaagt tacctgttat ttatcccacc aa
//
LOCUS       DADMFL010000238         2961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.246, whole genome shotgun sequence.
ACCESSION   DADMFL010000238 DADMFL010000000
VERSION     DADMFL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.246"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JNZ27_004160"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JNZ27_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY8666243.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRMVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JNZ27_004161"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JNZ27_004161"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY8666244.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2961
                     /locus_tag="JNZ27_004162"
     CDS             2603..>2961
                     /locus_tag="JNZ27_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8666245.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          661 a          703 c          779 g          818 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgatttt gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcatggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggt gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa accatgtatg t
//
LOCUS       DADMFL010000239         2961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.247, whole genome shotgun sequence.
ACCESSION   DADMFL010000239 DADMFL010000000
VERSION     DADMFL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.247"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(281..553)
                     /locus_tag="JNZ27_004163"
     CDS             complement(281..553)
                     /locus_tag="JNZ27_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666246.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            642..821
                     /locus_tag="JNZ27_004164"
     CDS             642..821
                     /locus_tag="JNZ27_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666247.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            873..1067
                     /locus_tag="JNZ27_004165"
     CDS             873..1067
                     /locus_tag="JNZ27_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666248.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1866..2202)
                     /locus_tag="JNZ27_004166"
                     /pseudo
     CDS             complement(1866..2202)
                     /locus_tag="JNZ27_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          960 a          635 c          588 g          778 t
ORIGIN      
        1 acccgagtcc ctttcattcg cccaaccgat accatacttt atgccaacag aaagaatgaa
       61 tgaaccgcgc ctaaaatcag caaggacaca tggcacaaag tgcgatatgt ctcacattta
      121 accccagtgg gggcaacaag tataaaaccg ttttttgatt aagtggtcga atatgaaaag
      181 gggaaggttt atgtgaaaaa tacctgtgtt ttctttgaaa acacacaaca gtatcaggtt
      241 atttatgttt atacagtgcg ttccaaactg acctgaataa ttacgcaagt tgcaacaata
      301 aaacattttt gccgtagtgt gaggtactcc ggtaaattgc tttccaggtg cagacaatga
      361 ctaacgaaga actacaggca atcgcattat ccatgcttga gcgccagcgc gaaaaagtac
      421 accagcaacg agagtctttg cttaacccat ctccattcac caggccaaaa cgattggagc
      481 ttgaagcatt cttggacaaa tacccacggc ggcttaaatc aggcaaaacc agaccaccag
      541 gctgatagct cacagtttgt tctccgattc aataaaacgg ataagaacca tcagcgcggt
      601 agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta tcaccagaag
      661 acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc
      721 aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg
      781 acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac
      841 agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg
      901 agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc
      961 cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa
     1021 tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat
     1081 attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct ttatatccac
     1141 tgacgagctt gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac
     1201 ggcgtacaag cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt
     1261 gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc
     1321 gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat
     1381 ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg
     1441 ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta
     1501 accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg
     1561 atgctatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
     1621 agggttggca atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca
     1681 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac
     1741 actgctaaaa cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca
     1801 gaatctgcat tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac
     1861 ctactctata cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta
     1921 gttgtcacaa tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt
     1981 aattctgcgt tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca
     2041 gttccccttt ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc
     2101 ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc
     2161 agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat
     2221 tgagtcgaaa ataagcacta ctccatacag gataattaga ggtcagtttg attattcaca
     2281 attcatcatc agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata
     2341 attattataa taacggcttt aattggaata catatattac aacgtattat atataattgg
     2401 tattctggga actatattct caaaatacag tagaaacgag gtatgtttct ggtggaaagg
     2461 acagtgggat taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg
     2521 gagggaatac cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa
     2581 tagttagcgt tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta
     2641 tgcatgacct acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca
     2701 aacacaatat tcatagtatg gcgatgccta cgccagcaag aatagcgcca ataatattgg
     2761 gaatataata gacaccagac gcacaggcat ctcactcctt aacaaacaat aatcaggata
     2821 tttactttta cccagctaac tgtttacacc caaagtgcac acaattaacc attcaataac
     2881 aaatggcaat atccatagcc atacgacttt accttgtaat gttcggtatt tctttataat
     2941 tattctggga aatctatcat t
//
LOCUS       DADMFL010000240         2871 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.248, whole genome shotgun sequence.
ACCESSION   DADMFL010000240 DADMFL010000000
VERSION     DADMFL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.248"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JNZ27_004167"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JNZ27_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY8666249.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JNZ27_004168"
     tRNA            complement(621..695)
                     /locus_tag="JNZ27_004168"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(771..1703)
                     /locus_tag="JNZ27_004169"
     CDS             complement(771..1703)
                     /locus_tag="JNZ27_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8666250.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1997..2752
                     /gene="mlaA"
                     /locus_tag="JNZ27_004170"
     CDS             1997..2752
                     /gene="mlaA"
                     /locus_tag="JNZ27_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY8666251.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          694 a          698 c          690 g          789 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      721 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      781 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      841 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      901 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      961 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatgggtg
     1021 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
     1081 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     1141 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1201 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc agtcccgaga
     1261 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1321 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1381 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1441 ggcacacctt ccagttcgac atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1501 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1561 ccatcctggc ggatatgctc atgaattgcc atcgctcggg aggggagtcg gtcttcatct
     1621 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1681 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1741 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggttgcgt
     1801 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1861 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1921 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1981 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     2041 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     2101 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     2161 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac
     2221 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2281 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2341 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2401 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2461 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2521 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2581 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2641 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2701 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2761 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2821 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga t
//
LOCUS       DADMFL010000241         2786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.249, whole genome shotgun sequence.
ACCESSION   DADMFL010000241 DADMFL010000000
VERSION     DADMFL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.249"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            23..985
                     /gene="yeeE"
                     /locus_tag="JNZ27_004171"
     CDS             23..985
                     /gene="yeeE"
                     /locus_tag="JNZ27_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY8666252.1"
                     /translation="MYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEAGAFPWLG
                     TVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPHASGLNQT
                     LQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPRRTGIAHI
                     LFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAGDVKYINW
                     GVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGL
                     VMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /locus_tag="JNZ27_004172"
     CDS             999..1226
                     /locus_tag="JNZ27_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY8666253.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNZ27_004173"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNZ27_004173"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY8666254.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRKRLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQIYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          642 a          658 c          740 g          746 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgttct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaatct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgcttgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaata
//
LOCUS       DADMFL010000242         2759 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.250, whole genome shotgun sequence.
ACCESSION   DADMFL010000242 DADMFL010000000
VERSION     DADMFL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.250"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(297..1214)
                     /locus_tag="JNZ27_004174"
     CDS             complement(297..1214)
                     /locus_tag="JNZ27_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY8666255.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIIFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKDKK"
     gene            complement(1211..1573)
                     /locus_tag="JNZ27_004175"
     CDS             complement(1211..1573)
                     /locus_tag="JNZ27_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8666256.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSAIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(1835..>2759)
                     /locus_tag="JNZ27_004176"
     CDS             complement(1835..>2759)
                     /locus_tag="JNZ27_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8666257.1"
                     /translation="SRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEENWKLRAE
                     ACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKR
                     IRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEE
                     SKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTA
                     WKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNH
                     LTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          770 a          610 c          554 g          825 t
ORIGIN      
        1 aaggttgttt tttctctgaa agatagagca aatgccaata attaatgatg aaaacattgc
       61 tgcattaatt aaagaccaag cataaaaatg attaataaga tttattaatg catattcaat
      121 ctatgttcgt gatgaccatg tgaaatatct atctatgagc cacttgatgt gatccggttc
      181 tgatattgat ttctcggaaa accaaatgtc atctgatact agtgtatatt gatttaaatg
      241 aagaatagtt aaaccaataa aaaaaagcaa aagaaagcat atgtaatttt atttaatcat
      301 ttcttatcct taataatata tttaggtcta ttttttactt caatatatat tctgcctata
      361 tattctccga gaacaccaat cccgatcagt tgcacgccac ccaagaaaag tattgataca
      421 agcagggagg gatacccgcg tactgggtta ccaaagacaa gggtgtctat aatcatccat
      481 gcaccatata aaaacgaaat gcttgcaaca aacaagccta tataagtcca tacgcgaaga
      541 ggaaaggttg aaaaacttgt gataccttcc agtgccagat tccataattt ccagccatta
      601 aattttgaga tgccagcaac acgctctgcg cgtacatatt caacgacatc tgtctgacca
      661 cccacccagc tcagtatgcc tttcatgaaa agattgcgct caggcaacag tttaatgttc
      721 tccacaacct cacgagacat gagtcgaaaa tctccgacat tttcctcgat ctttggggtg
      781 cttattttgt tgtgtaactt atagaaccac tcagctgtct tacgtttcag tcgtccatca
      841 gttgagcggt ctgaacgttt agcaagcacc atgtcagcac ctgcctgcca cttttcaata
      901 agatgaggga taacctcaat agggtcttgc aggtcgacat caataggaat tacagcatcg
      961 ccgcttgcat ggtctaaccc tgcaaataag gctggttctt taccaaagtt gcgtgtaaat
     1021 gacagcggaa caactagcgg gtctgaaaca gccagcgcgt taataattga ctctgtggca
     1081 tctttacttc cgtcatttat gaatataatt tctacttcat atggcttcaa ctcttggaat
     1141 tcacgtaccg tcttatagaa aacaggtatc gcttcttctt cattgaagac aggaacgaca
     1201 agagatattt tcatttcgca tccctaaaga caatgaactt tgaatagacg aaaccgcaca
     1261 ccaggctgat ggcggagaag gtgataagag tgactattgg aggaagtgag catttatcag
     1321 cagcccaacc aacaatcgca cttaatattc ccatgaatcc cacgtataac atgtagcgca
     1381 tcgctgtagt cgatgctttg aatgtgaatc ttgcattcgc gaagaagcta aagctcacag
     1441 ccactacgaa acctgtgaag tttgccaggg cctgactggt atgtgcggca tagatacata
     1501 caccaaaaac cacccagtgt ataagtgtgt tcagcacacc tatagatgtg tactttacaa
     1561 ataactttaa catttattta atcaatgagc tctgaaaggc atgaagtcta tcatccaagt
     1621 ctcaattgat cgatacttgc tatgtctgat gagacaaaac tgagacacat aaggcctcac
     1681 aatggcttgc aaggctttac atgttttgat gtggtgggac gtgagagcgc agtgttgatg
     1741 ggataatctc ttgaattaca agcggattct tataattcgt aatgcgaagg tcgtaggttc
     1801 gactcctatt atcggcacca tttaaatcaa taagttacac atcattagta ccttccttat
     1861 tttttgactg ggacaaattt gggaccgatg ggttcaggat cgagtctatt tgccgtgcgt
     1921 gttcggtaag gtgattaggt gcaaggtgag catatcgacg aaccatttcg atagactccc
     1981 agcctcccat ttcctgtaac actgacaacg ggactccggc ttgaaccagc caacttgccc
     2041 aggtgtgtct caagtcgtga aatctgaaat catcaatacc agcccgtctc agcgccgctt
     2101 tccaggctgt gtttgcgtca taccgcatct tccttactgt tggcgctttc gttccgtctg
     2161 gtttggtaca gctttccttg tacacaaata cccaacggtg atgatttccg atttgttttt
     2221 tcaaaacgcg acatgcagta tcattcagcg caacgccgat tgcgcggttt gatttactct
     2281 cttccgggtt tatccatgcc acccggcgct gcatatctat ttgttgccat tcaaggttga
     2341 tgatgttcga gcgtcttaag cctgttgcca gtgcaaattc aacaacagac tttaatggct
     2401 ccggacattc atcaatcagc ctttgtgctt catggggctc cagccagcgg atccgtttat
     2461 tctttggttg aggcacttta ataattggtg ccttatccag cattttccat tcacgctctg
     2521 cggctcttag tagggccttt ataaatgaaa gatgcgtagc cttcgttaca acggacgctg
     2581 gttttggcgt gtattctgga acaggtttcc ctttttttct gcatgcttct gccctgagtt
     2641 tccagttttc ctcatgacgc cggttcgtca ttttctgcat tgctgaataa atttttgatt
     2701 cagtaatgtc tcttagttgc attcctgcga aatgttgaag ccagaatccg atccggctt
//
LOCUS       DADMFL010000243         2662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.251, whole genome shotgun sequence.
ACCESSION   DADMFL010000243 DADMFL010000000
VERSION     DADMFL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.251"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..565
                     /locus_tag="JNZ27_004177"
     CDS             <1..565
                     /locus_tag="JNZ27_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8666258.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JNZ27_004178"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JNZ27_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY8666259.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNZ27_004179"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNZ27_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY8666260.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JNZ27_004180"
     CDS             2167..2403
                     /locus_tag="JNZ27_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY8666261.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2442..2528)
                     /locus_tag="JNZ27_004181"
     tRNA            complement(2442..2528)
                     /locus_tag="JNZ27_004181"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Leu,seq:cag)
     gene            complement(2563..2649)
                     /locus_tag="JNZ27_004182"
     tRNA            complement(2563..2649)
                     /locus_tag="JNZ27_004182"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2613..2615),aa:Leu,seq:cag)
BASE COUNT          677 a          604 c          681 g          700 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg catgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctatcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact
     2461 tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc
     2521 accttcgcac agtcatctta ctttttttga tatcgcctcg tttggtgcga ggggggggac
     2581 ttgaaccccc acgtccgtaa gaacactaac acctgaagct agcgcgtcta ccaattccgc
     2641 caccttcgcc cagtgcgagc aa
//
LOCUS       DADMFL010000244         2650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.252, whole genome shotgun sequence.
ACCESSION   DADMFL010000244 DADMFL010000000
VERSION     DADMFL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.252"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1475
                     /locus_tag="JNZ27_004183"
     CDS             <1..1475
                     /locus_tag="JNZ27_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY8666262.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTRILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT          681 a          560 c          621 g          788 t
ORIGIN      
        1 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
       61 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
      121 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
      181 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
      241 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
      301 gggagttcat ctccgctggc gacaaccaga cgcggattct ggcgacagac gattatatat
      361 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
      421 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
      481 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
      541 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
      601 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
      661 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
      721 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
      781 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
      841 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
      901 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttaccg cctctgacag
      961 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
     1021 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
     1081 ctatagagcc cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
     1141 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
     1201 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
     1261 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
     1321 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
     1381 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
     1441 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
     1501 cattgaaccg gtgagggccg ccccctttat tactcagggg gggctttcaa cgttctgaat
     1561 taagatgttt tatgactcac tgagcaggat gtgagttcca acgtaatctc cgctattttt
     1621 actgtttttc atttgctgcc tcatctatcc cttcgccttg cctctgagtt gcgttaaagg
     1681 ctgatgtact gcctttaacc cgttcactac atagcctttt tgtcaatcat catcccgaag
     1741 agcgttgcac tgtgccttcc atagctgtgg ttgtcctctc aatctgtcag cccatttatg
     1801 gaaaatctta acgaagcaac aatgagatat gtaaaaaaag ggccgattta atcattcatt
     1861 aagacaggtc attttttctt actgtgaggg cggtaagctt ttctgtcggt aacaggttgg
     1921 gagatgctgg tattatgaga tttttctacc gtcctgcggc ggaatatgtg aaaaatagaa
     1981 atgattatta ttattgcatg gttattttta gtttgtgatg gtggagaata atcctattcc
     2041 cgtgaccttg ttttttacat tttatttaac tgcatggtat atgcattttg gacggtgaaa
     2101 gcaagaagct attaggcgct ataggtcttt tgacggtttt tgaatttccc ctcctgatat
     2161 ctctgttata tttttgtgaa tttctccaat ccaaatcaaa gtcccaataa gtcatggatg
     2221 catggttaaa ataactatca tttgtgtaac ctttggtgct tataactttt ctttattctt
     2281 ttttcaggat tacctggagg gattgaccaa aaaaacattc attgaatgat atttttctaa
     2341 ttaatgaggt tttctaagct cgttgaaata aaaaatatat tttaatcatg gatttgcatg
     2401 cgtttctttc gcttttttta cacaagttga cattgaactc agcaactata agaatagcca
     2461 catcccagac ggtgaccgat aaaggaaata ccgttaccat atttaaatga attggaaaat
     2521 atcaccgatt tgaagtcgac tcgttcgggt aattattaat actgaatctt taatgcttat
     2581 ttcttaagct tgttaacaac gggaatgaat aatgaaagtg tccaaaattg cactggcaat
     2641 gggtcttagc
//
LOCUS       DADMFL010000245         2531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.253, whole genome shotgun sequence.
ACCESSION   DADMFL010000245 DADMFL010000000
VERSION     DADMFL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.253"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..1445
                     /locus_tag="JNZ27_004184"
     CDS             <1..1445
                     /locus_tag="JNZ27_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY8666263.1"
                     /translation="FRQLALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELI
                     PGYFQITVVPKLAVYDPTVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRP
                     GHDYYFYVRSVNTVGKSAFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQ
                     LAPDLTEIRTSITNVSNEITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQ
                     MKDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMND
                     VFLKRLTAPTITSGGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVL
                     GKLSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRG
                     AKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSA
                     WLVNGWYPTASISDLLVVVMKKATAGITIS"
     gene            1513..2112
                     /locus_tag="JNZ27_004185"
     CDS             1513..2112
                     /locus_tag="JNZ27_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY8666264.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2176..>2531
                     /locus_tag="JNZ27_004186"
     CDS             2176..>2531
                     /locus_tag="JNZ27_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666265.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGA"
BASE COUNT          611 a          573 c          761 g          586 t
ORIGIN      
        1 gcttcaggca gctggcgctg gggaattaca gtctgacagt ccgggcggta aatgcccggg
       61 ggcagcaggg cgatccggcg tcggtatcgt tccggattgc ggcaccggca gcgcctgtca
      121 ctattgaact gataccgggg tattttcaga taacggtggt cccgaaactg gctgtatatg
      181 acccgacggt gcagtttgag ttctggtttt cggaaaagcg gattgcggat atcaggcagg
      241 ttgaaaccag cgcccgctat cttggcacgg cgctgtactg gatagccgcc agtatcaata
      301 tcaggccggg ccatgattat tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg
      361 catttgtgga ggccgtcggt cgggcgagcg attatgcgga aggttatctg agtttttata
      421 aagggttgat caataaaacg catctcggca aggagttgtg gacgcagatt gataacggtc
      481 agcttgcgcc ggacctgact gaaatcagga cgtccattac gaatgtcagc aatgaaatca
      541 cgcaaaccgt caataaaaca ctggaaaatc agagtgctgc aatccagcag atacagaaag
      601 ttcaggttga tacaaataat aacctgaaca gcatgtgggc tgtgaagctg caacagatga
      661 aggacggacg cctttatatt gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa
      721 tgcagagtca ggtgctgctg gcggcagaca ggattgcgat gattaatcct gcgaatggca
      781 acacaaagcc gatgtttgtt ggtcagggcg atcagatatt catgaacgac gtgttcctga
      841 aacgcctgac ggctccgacc attaccagcg gcggtaatcc tccggcattt tccctgacac
      901 ctggcggacg gctgacggcg aaaaatgccg atatcagcgg taacgtgaac gcgaactccg
      961 ggacgctcaa caacgtcacg attaataaga actgtcgggt tctgggaaaa ttgtccgcga
     1021 accagattga aggcgatctc gttaaaacag tgggcaaacc tttcccacgg gactcccggg
     1081 caccggagag gtggccatca gggaccatta ccgtcagggt ttatgacgat cagccgtttg
     1141 atcggcaaat tgttattccc gcggtggcgt ttcgcggtgc taaacatgag cggaagaata
     1201 acaatattta ttcgtcatgc cgcctgatag tgaagaaaaa cggtgctgaa atttataacc
     1261 gaacgaccct ggataatacg ctgatatata cgggtgttat tgatatgcct gccggtcacg
     1321 gtcacatgac gctggagttt tctgtatcgg catggctggt aaatggctgg tatcccacag
     1381 caagtatcag cgatttgctg gttgttgtga tgaagaaagc cactgcaggc atcacgatta
     1441 gctgaatttt ataacccaga tacgggcacc agaaatggtg ccttttttat tgcagaaaag
     1501 cgagaggtaa ttatgcgtaa agtttgtgca gccattttgt ccgcagccat ctgtctggct
     1561 gtatccggtg cgcctgcatg ggcgtctgaa catcagtcca cgctgagcgc cgggtatctt
     1621 cagccccata ctgatatgcc aggcagcgat gacctgaagg gcattaacgt gaaataccgt
     1681 tatgagttta cggacacgct ggggctggtg acgtcattca gctatgccaa cgctgaagat
     1741 gagcaaaaaa cgcattacag cgatacccgc tggcatgagg attccgtgcg taaccgctgg
     1801 ttcagcgtga tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg
     1861 gcgggcgtgg cttacagccg tgtgtcgacc ttctccgggg attatctccg cgtaactgat
     1921 aacaagggga aaacgcacga tgtgctgacc gggagtgatg acaaccgcca cagcaacacg
     1981 tctctggcgt ggggggctgg cgtgcagttt aacccgaccg aatccgtggc cattgacctt
     2041 gcttatgaag gttccggcag tggcgactgg cgcactgacg gtttcattgt gggtgtcggt
     2101 tataagttct gattagccag gtaacacagt gttatgacag cccgctggtt caggcgggct
     2161 ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac tgaaagatgg ggcgggtaaa
     2221 cctgtcgtaa attgtgcgat tgaactgcgg gccagaagaa ccagtccgac cgttgtggca
     2281 cacgttgttg ccacttgcgt gacggacaat aacggtgctt atgtgattga ggctgagccg
     2341 gggtattacg aggttgcgct tcactgtaac ggctggcagc caacccgtgt cggggatatt
     2401 gatgtggcac cgactgatgc accggggaca ctgaacgcgt ttctgaatgc accaaaggat
     2461 ggtgatttac gtccggaggt gatgaagcgc tttgaggaaa tggtggcgca ggcgcagcag
     2521 agtgccgggg c
//
LOCUS       DADMFL010000246         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.254, whole genome shotgun sequence.
ACCESSION   DADMFL010000246 DADMFL010000000
VERSION     DADMFL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.254"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            155..1108
                     /locus_tag="JNZ27_004187"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JNZ27_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNZ27_004188"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNZ27_004188"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY8666266.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          692 a          615 c          560 g          636 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttac tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADMFL010000247         2484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.255, whole genome shotgun sequence.
ACCESSION   DADMFL010000247 DADMFL010000000
VERSION     DADMFL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.255"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            46..297
                     /locus_tag="JNZ27_004189"
     CDS             46..297
                     /locus_tag="JNZ27_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8666267.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            299..2392
                     /locus_tag="JNZ27_004190"
     CDS             299..2392
                     /locus_tag="JNZ27_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8666268.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
BASE COUNT          688 a          579 c          686 g          531 t
ORIGIN      
        1 tccacgcgaa tatccgcgcg ggtaattttg tagggtaaag gaaagatgag acaaagagaa
       61 gatagtggcc gcgattggcc ttcgctgatg attcagcaag cactcaaaga taatgggtgg
      121 tcttcctttc gggagctttc actccatgcc ggttatgctc aggattcact gaaagcggta
      181 gttcgtacgc caaacaaaaa atacgaacgt gttatttctg acgctatagg tgtaccgcca
      241 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc gtcgtgatat
      301 gcagtcgtac gttaccgtaa atgaccttct cggtgttcct ggaatgccag ccaccgcgaa
      361 aggcatccga caggcgttac agcgtttttc tcgtgctctt ggtgacgtta gtcgccgtcg
      421 agcaggtaca aaagccattg agtaccatat cgattgcctg cctgaggtaa cccaaaaggc
      481 actgcgtgaa cgctatgtgg aacaactggt ggctacagag aacaacgtct ccgaagtgaa
      541 gaccgttacc aggacggcgc gtaaccctga tgctgttcag gcagttgaag catatcgggg
      601 ttctccgcaa ctaatggaag aacgcctgaa tgccctgact gaaaatcagc gccgggtgtc
      661 tgaagcaaga gcggcgctgg cagtggaagt gctgaagctg gaaagcgccg gtaacctcgg
      721 gcgactgaaa gccattaact ttctggttga aaaagcccgt aaaggtgagt tgccggaacg
      781 actgcaacag gctgcgatta acgctaatgc aaaacgtggc actaatcgca ccatcagccg
      841 tgatccgctt taccagtggg tactgaaata caaccggtcg cagaacgccg cagaacgact
      901 gttgttactg gcaccaggaa aacgcgacga aataaaaccg gaagaaatta gctggctgcc
      961 tgaatttctg gcgcagtatc gtcaggctaa cggcagacct atgtcggaag cctacgagga
     1021 ttttgtcacg gaatggcaac gtcgctatgc ggacgaaccg tacatgctgg aggtgatgcc
     1081 ttcctatgat gttgtccggt atgccatgaa aaaattgccg gaagtggtga aacaaaaagg
     1141 acgcgttacc ggcagtgaat accgccagct tgagggattc acccgccgcg actggacagc
     1201 tatgccggtg aattatgtct ggattggtga cggccacggt atgaagctga aatgtgcgca
     1261 tccgatccac gggcgtccat tttcaccgga agtcacgttt gttatcgacg gtggaacccg
     1321 gtttgttgtt ggctggagtc ttgatcttgc ggagaacgtg tttgctgttg ccggggcgat
     1381 acagcacgga attcgcaatc acggaaaacc cttcctgtat tactcggata atggttccgg
     1441 tgaaacggca gatatgctgg ataaagagat cgtcgggatt ctgccccgtc tgggtattaa
     1501 ccaccccacc ggtattgccg gaaatccgca gggacgcggc atcattgaac gtctgaaccg
     1561 gacgctgccg atgcgtatag ccagacggta tcgcacctat tttggcaagg gggcagaccg
     1621 ggaaacactg cgtgtactca atcgcgatct gcgttcagca tttaatgccc tgcaacagga
     1681 caaaccgctg aatgcccgcc agaaatctgc aatgcgcgag ctgccgtcct ggtcagaact
     1741 gattgaggct attcgtgaag gagtggaatg gtacaacaac cgcccgcatt ctgaactgcc
     1801 gatgaaacca aacgggaagc attacagccc ggctgagttc agaaaaaaac gcctggcgga
     1861 agaaaacacc gaaattgaat ggctgagtga tctcgaactg cgcgacatgt tctgcccgat
     1921 ggtggagcgt ccggtaagac gctgtgaaat caaatggctt gggaatattt attacgcgcc
     1981 ggagctgcgt gatgaacatg gtcgcaaagt gcttatcagc tatgacattc atgatgcaga
     2041 gcgaattaca gtgcgccgta aggatggcag ttttatttgt gaggcgatat ggaacggcaa
     2101 taaacgcgcc gccttcccgg tcagcgccga ctattacaaa caacagcagc gtattaaagg
     2161 aatgcacaaa cgcgcagaag aaaaaatccg tgatgccgtg gatgaaggta ttcaggttat
     2221 tgaacaaaaa acgacagaac cctggctgga taatgtttat cgacccgttg gcaatgtgat
     2281 gaccgttcag caacaggaat atgaagagga gcacgacgaa gaatccgaac gcaatttcag
     2341 gcggggatta cagaaactca ttgccatgca ggacgatgac ccactggcct gaataaaaaa
     2401 acagtccgca gcaacggact gttaaacagg taaataaagc tgatttgaaa taaccgagga
     2461 aacaatatga ccgatattaa tgat
//
LOCUS       DADMFL010000248         2475 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.256, whole genome shotgun sequence.
ACCESSION   DADMFL010000248 DADMFL010000000
VERSION     DADMFL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.256"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            30..1082
                     /locus_tag="JNZ27_004191"
     CDS             30..1082
                     /locus_tag="JNZ27_004191"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY8666269.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1158..>2475
                     /locus_tag="JNZ27_004192"
     CDS             1158..>2475
                     /locus_tag="JNZ27_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY8666270.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSAYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPDCRIAGLPDCRIAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAG
                     LAELGPMKIREKMLLGVFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVV
                     KNKGGWNTLIWYGGIIGLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSII
                     VRYFFASGSAYIVAMLPVFAMLANVSGAPLMLTALALLFSNSYGGMV"
BASE COUNT          586 a          557 c          640 g          692 t
ORIGIN      
        1 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
       61 gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc
      121 gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg
      181 gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg
      241 ctgatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc
      301 ctaactgcgg caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg
      361 cagcgacttc gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg
      421 aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg
      481 gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa
      541 ccggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct
      601 gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt
      661 atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc
      721 ggctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac
      781 ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc
      841 attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat
      901 tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg
      961 aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt
     1021 tacgcgcatc tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct
     1081 gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag
     1141 aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga
     1201 tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc
     1261 tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg
     1321 tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg
     1381 cgtttaagac caccgccgta ttaagcgctt actcttcagg tactacctgg ctggtgttct
     1441 cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc
     1501 tgctgattgg taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg
     1561 atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac
     1621 cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg
     1681 tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct
     1741 ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc
     1801 aaattagctg gggtggatgg gcgctggccg ccggattgcc ggattgccgg attgccggat
     1861 tgccggattg ccggattgcc ggattgccgg gcatcattat gctgctggtc accccgctgg
     1921 taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc atcgctaaag
     1981 cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc ggtgtctttg
     2041 tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa tccactgtgg
     2101 caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa gacgtggtta
     2161 aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc ttaagctcct
     2221 tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat aacctggcat
     2281 ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc atcgtgcgtt
     2341 atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt gccatgctgg
     2401 cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc tccaactcct
     2461 atggcggcat ggtta
//
LOCUS       DADMFL010000249         2400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.257, whole genome shotgun sequence.
ACCESSION   DADMFL010000249 DADMFL010000000
VERSION     DADMFL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.257"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(334..600)
                     /gene="ospE2"
                     /locus_tag="JNZ27_004193"
     CDS             complement(334..600)
                     /gene="ospE2"
                     /locus_tag="JNZ27_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY8666271.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(782..1183)
                     /locus_tag="JNZ27_004194"
                     /pseudo
     CDS             complement(782..1183)
                     /locus_tag="JNZ27_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1612..2400
                     /gene="virF"
                     /locus_tag="JNZ27_004195"
     CDS             1612..2400
                     /gene="virF"
                     /locus_tag="JNZ27_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY8666272.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          739 a          423 c          446 g          792 t
ORIGIN      
        1 gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat atatttctcc
       61 atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt ctactaaaag
      121 gtatttaagg tcattgcgtc cctgcactcg aataatggtg aagaaagcat gataaatata
      181 tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct gaaatagaat
      241 tactgctcta ttttttctgt ttccagaaat tattagtaac aaaccctgat atattatttt
      301 gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc gttggctttg
      361 tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg tagcagtgaa
      421 tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag ctgctttttt
      481 attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacag gatttgatac
      541 ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt gtgtaagcat
      601 attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata gtcggctaaa
      661 tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg gagccagaaa
      721 aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca gtcgttgtat
      781 cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa cccaacgcac
      841 tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc aatgccgttc
      901 ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac cgttctgcta
      961 ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc aactctctgg
     1021 cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact ggattggccg
     1081 ttccggtcct ttatcgccat atcgctcctg acatgacatc ctgcaccgtt gctttgtcat
     1141 aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga acgtaacgct
     1201 gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc aacaccaacc
     1261 ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga tattatgatg
     1321 ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg cctaagaaaa
     1381 gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg ttgcacagag
     1441 aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa tataggaaaa
     1501 attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt acagaaattt
     1561 cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt tatgatggat
     1621 atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat tattttatat
     1681 gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat cgatgaaggg
     1741 caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa atctgatagc
     1801 attaatccat ttgagattat aagccttgac agaaatttat tattaagcat tattagaata
     1861 atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg gttaaacaaa
     1921 aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc tataaaagag
     1981 atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc tgtttctgat
     2041 gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc tgatcagata
     2101 aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat ttcaaataac
     2161 ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt aacatttcaa
     2221 caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa tagtcaaagc
     2281 tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt tataaggaaa
     2341 tttaatgaat attatggtat aactccaaag aaattttact tatatcataa aaaattttaa
//
LOCUS       DADMFL010000250         2385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.258, whole genome shotgun sequence.
ACCESSION   DADMFL010000250 DADMFL010000000
VERSION     DADMFL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.258"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..308
                     /gene="capU"
                     /locus_tag="JNZ27_004196"
     CDS             <1..308
                     /gene="capU"
                     /locus_tag="JNZ27_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8666273.1"
                     /translation="ASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQNNQT
                     GTLLPAGNKHAWMCALNNFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
     gene            313..1263
                     /gene="virK"
                     /locus_tag="JNZ27_004197"
     CDS             313..1263
                     /gene="virK"
                     /locus_tag="JNZ27_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8666274.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1328..2272
                     /gene="lpxM"
                     /locus_tag="JNZ27_004198"
                     /gene_synonym="msbB"
     CDS             1328..2272
                     /gene="lpxM"
                     /locus_tag="JNZ27_004198"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8666275.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          699 a          459 c          536 g          691 t
ORIGIN      
        1 ttgccagtct ggtggttctg ccttcagaaa acgaatcttt tggtatggtg ctggcagaag
       61 catcggcatt ttctgtgcct gtagtggcca ctcagattgg tggaatccct gaggttattc
      121 agaacaacca gaccgggaca ttgttaccag caggtaataa gcacgcatgg atgtgcgccc
      181 tgaataattt ttttaatgac cctgggcgtt tttatcagat ggctcgcctg gcaaaacagg
      241 atatagaaga gcggtttgat attaataaaa ctgcgttaaa aatactcaca ttagcgaagc
      301 aaaagtaaca atatgttttc tataagtaac ttatcattta tcggtttcct taaaaggatt
      361 attttttcct cagattcact accggggaag tgggaacaca gaaaatttcg gttcatgtac
      421 attttgcgat gttctataaa tccggttgtc agtattcgat attattatga actgcgttcc
      481 ttgccgtgca ttgaggatat tctggcaata caccccacgt tgccagcaag aattcatcgc
      541 ccttatttgc ataaaggggg gcgcgcctgg acccgtggac aatatattct tgagcattat
      601 cgctttgtgc aaaatttgcc agaaaaatat tctaaatttt tgtttccaca aaaatcggta
      661 tcattagtcc aattcatcgg gaaagatgga gagaattttg atatccagtg ttctcccagt
      721 ggttttgacc gggaaggaga gttgatgttg tcattgtttt acaataaaac agttattgcc
      781 cggttaacat tttcagtcat tctgactcaa aatggacaca ttgcttttat tggcggatta
      841 cagggagccc ctaaaaatac cggacctgat gttattcggt gcgcaacccg ggcctgttat
      901 gggattttcc caaaacggat tatttttgaa gcattttgtg cgttgatgaa agcttgcaat
      961 atctctgagt gtctggcagt cagtgagcac agtcatgttt tccggcaatt gagatactgg
     1021 tatcagaaac gcaaaacctt tgttgcggtc tatagtgatt tctgggagtc tgtagcagga
     1081 aaaacctgtg gtgactggta cagattacca acccaggtga ttcgaaagcc gctaagcgat
     1141 atagccagta aaaaacgctc tgagtaccga aaacgatacg ctttgctgga ttatatccat
     1201 gaaactacta tccgctcttt ggatgcatat cctgtacact cagaacatca gaatttaaat
     1261 taaggtttga tgtatcctct gaataaaaca gtaatgataa ttttggtaga ggcattcgca
     1321 ctaaataatg aaaaaatata aatctgagtt tattcctgaa tttaagaaaa attacctttc
     1381 ccctgtttac tggtctacat ggttcctttt gggaatgatt gcaggtattt caatgtttcc
     1441 cccttcattc agagatcctg tcttggcaaa aatagggcgt tgggcgggca gattgagcaa
     1501 aaaagctcgt cgcagggcga cgattaattt atcgctttgt ttcccagaaa agaatgatac
     1561 agaacgggaa ataattgtcg acaaaatgtt tgccacagca ttgcaatcca tagtgatgat
     1621 ggcagaactg gcaattcgtg gtccggaaaa gttccagaaa cgagtgttct ggaaaggcct
     1681 cgaaattctt gaggaaattc gacacaataa tagaaatgtg atttttctgg ttccccatgg
     1741 ctggagcgtg gatattcctg caatgttgct ggcagctcag ggaaaaaaaa tggccgccat
     1801 gtttcatcag caacgaaatc cagtgattga ttatgtctgg aattcagtac ggcgtaaatt
     1861 cggggggcgc ttacatgccc gggaggatgg gataaaacca tttattcagt cagtacgcca
     1921 gggatactgg ggatattacc tcccagatca ggatcatggt cctgaataca gtgaatttgc
     1981 tgattttttt gcgacctata aagcgacatt accaatcatt ggacgtctga tgaacatcag
     2041 tcaggctatg attataccac ttttcccggt ttatgatgaa aaaaaacatc tcctgactat
     2101 tgaaattcgg ccaccaatgg atgcttgcat tgccagcgcg gacaataaaa tgattgcccg
     2161 acaaatgaac aaaacagtgg aaattttggt ggggccacat ccggaacagt atgtctgggt
     2221 tttaaaattg ttaaaaacac gcaaatcaaa cgaagcggac ccgtaccctt gaaattataa
     2281 agtataagta tttccactag ttgtttgtaa gcgtacagcc tgaaccgtct ggtcagaatc
     2341 tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg ggaac
//
LOCUS       DADMFL010000251         2373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.259, whole genome shotgun sequence.
ACCESSION   DADMFL010000251 DADMFL010000000
VERSION     DADMFL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.259"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..823)
                     /locus_tag="JNZ27_004199"
     CDS             complement(<1..823)
                     /locus_tag="JNZ27_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8666276.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JNZ27_004200"
     CDS             complement(1424..1672)
                     /locus_tag="JNZ27_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8666277.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JNZ27_004201"
     CDS             complement(2027..>2373)
                     /locus_tag="JNZ27_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8666278.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          612 a          439 c          574 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc aggacgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADMFL010000252         2341 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.260, whole genome shotgun sequence.
ACCESSION   DADMFL010000252 DADMFL010000000
VERSION     DADMFL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.260"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..122
                     /locus_tag="JNZ27_004202"
     CDS             <1..122
                     /locus_tag="JNZ27_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666279.1"
                     /translation="QPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(255..767)
                     /locus_tag="JNZ27_004203"
     CDS             complement(255..767)
                     /locus_tag="JNZ27_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8666280.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            962..1726
                     /locus_tag="JNZ27_004204"
                     /pseudo
     CDS             962..1726
                     /locus_tag="JNZ27_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1677..>2341
                     /locus_tag="JNZ27_004205"
     CDS             1677..>2341
                     /locus_tag="JNZ27_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY8666281.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          662 a          522 c          592 g          565 t
ORIGIN      
        1 ctcagccgaa acctccagcg cctccggcat gggcaatgat gccaccgtcg aactctctcc
       61 agttgctgga cgaaacgttc tcggtatccg ggacggaatc atcagcgacc agacagcatt
      121 gagaatgctt caggagtaca tcaggatcca atgccttggg gggtagcggt aattttactc
      181 attatccttc aaatcagatt ctgttgtcag aggaatgggg gaagctggat tcgcaagagc
      241 tttttatggg cagtttagct aaaaaaaggc aacacgaagc taaaactacc aacataaagt
      301 gctgaccagc tagcagctga aatcagactc actgtataca ctttcactgg atgaagttgc
      361 aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg cgaagtgaaa
      421 agtattgaca cagagttatt ttgcaactga aggcgaaccc gttctattgt attggaacgg
      481 ctcgatatta tacatgcaag ttgagggata tgcccaatta ttataccaag atggtaatta
      541 acgatagtac cgcaccatgc gccactcatg accgtgattc cagcttccca tggtgaaaga
      601 gtcgtcttgc tgactgtaat tacagtggcc atcataattg atgctggtgg caatatcgcc
      661 gaaatgagta aagtggattt tgtgaacgct attataaaaa gaagccccca taggcgatat
      721 ggatgaagta caaaatagtt catcaaagca tttatccact ccatcaatgt ctttccttct
      781 gtgaattttt ctttgctatt taaccgtgat acgacttaat ttaggcttaa acaccttaca
      841 tttacaaggt actcctgtgg ggggccctgc cacggagcgt cgcaggagtg agtgatgagg
      901 aagaattaaa gcaacctatt gtgacaggca acgataacag taaatcacag gaagcataat
      961 catggcaaaa ccggactggg aggccatcga atcggcatac cgggccggag tccttagcct
     1021 ccgtgatata ggcgagaaat acggcgttac agaaggggct atcaggaaga gggccaaaaa
     1081 gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc aaaaaaggaa
     1141 agtgcgtacc agcagaaagc ctgccattac tggccttaca caaaaaagta cgcaactaaa
     1201 aacagaatct acaccggata cgaaaccgat acgcggaatg cgtaccgatc ccccgactaa
     1261 cccattccaa cccggtaacc agcaggcatt aaaacacggt ggttatgccc gtcgccttct
     1321 gctcaaagat gaggtgatag aggacgctaa agcgttgacg ctcgaggacg aattatttcg
     1381 ccttcgtgct aacaaccttg tcgctgcaga gaatattggt cggtggttgg tgtcgctgga
     1441 agatgctaat ggggactagg aaaggaagat gctgattgaa aatatcagcg ccgccgagaa
     1501 agcaatgatg cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg cgacggtagg
     1561 aaaaatattt gctgatacag cctaccgcaa ggccgccact gataaggtgt ctctggaggc
     1621 tgatcgtctt cgccgtgatg caggtattga tgatggcaat ggagagcgtg acctcaatga
     1681 cttctactct gacatccaaa ccgacgctga atccggcttt acgtagtttc tggactatgc
     1741 gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg gatgccgctg
     1801 gcattgccat atttctgtcg aataaataca ccctgcgttt ttgttgtgcc cgtcagatcc
     1861 agaataaaat cgaagagtcg gtgtataccc tgctcaaaat tcagatagac aggtttggtc
     1921 tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa ccgggttact ggctcggaat
     1981 ttgtttttta tggattatgg cgcaacatcg aagaaattaa gtcactggag gggatcgatg
     2041 tgttgtggct ggaagaagcc cacgcactga cggaatacca gtggaaaatt ctggagccaa
     2101 cgatccgtaa agagggttcg gaatgctggt tcatattcaa ccccggactt gttactgatt
     2161 tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac tctcatccgc aaaattaact
     2221 atgacgaaaa tccgtttctg tctgacacca tgcttaaggt tatcgacgcg gcgcgacgcc
     2281 gtgatccgga tggttttaaa catgtgtatg agggcgttcc ggagtctgat gatgatgcgg
     2341 c
//
LOCUS       DADMFL010000253         2320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.261, whole genome shotgun sequence.
ACCESSION   DADMFL010000253 DADMFL010000000
VERSION     DADMFL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.261"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..684)
                     /locus_tag="JNZ27_004206"
                     /pseudo
     CDS             complement(1..684)
                     /locus_tag="JNZ27_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            870..1712
                     /locus_tag="JNZ27_004207"
     CDS             870..1712
                     /locus_tag="JNZ27_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8666282.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1715..>2320
                     /gene="capU"
                     /locus_tag="JNZ27_004208"
     CDS             1715..>2320
                     /gene="capU"
                     /locus_tag="JNZ27_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8666283.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          623 a          467 c          530 g          700 t
ORIGIN      
        1 tccgttacgg cgaactgtcg ctcaagatga ttcgggatag caacgtgctc atgaccgcca
       61 cgcttatacc ggtgagtcgg ctgctggcaa ctgaccagcc ccagctcttt catgagtctg
      121 ccagcaagcc agcgccccat ctggtagcct ctctgggttg ccattgtggc gatgcttctt
      181 gctccggcag agccgtggct gatgccatgc agttcaagta cctggctgcg taatacagcc
      241 cgtctgccgt ctggtttttc aggacggttt ttccagtatt tgtagctgct gcgatggacc
      301 ccgaacacat ggcagagagt ggccacagga taacgcgccc tgagtttccc gattatcgaa
      361 aactgttcag ggagtctgac atcaagagcg cggtagcctt tttaatattt cattttccat
      421 ttcaatacgt tgtagctttt tcctgagctc acggatttca atttgttccg gggtaatggg
      481 ggaggctttt ggtgtttttc cctgccgctc atcacgtaat tgtttcaccc atcgcgtcat
      541 tgtggaaagg ccgacatcca tagcgctggc tgcatctgcc acggtgtatt tctggtcaac
      601 gaccagttga gcggattcgc gtttaaactc tgcgctgaaa tttctttttt cattatggca
      661 cctgtgttgt tctgaggtga gcatatcacc tctgttcagg tagctaaatt tagtgtgcca
      721 cctcaaggtt cttagtgagc cgtttttaaa agatatgata atatattatt gtttataaat
      781 tgttgatgtt tgatttagta gtatataaag ttcaccacct ttattaggct ttccttttat
      841 ctctacttac tgggtgccag aaactttaaa ttttaaacgg agggggcatt ttgtttaaag
      901 caaatcatgt tccggtatta atgtatcatc atgtcagcca ttgcccagga ctggttacac
      961 tttctcccga gacattccgg aaacaaatga aatggttggc tgaaaacaac tggaaaacac
     1021 tgtcatcaga tgaactggag tttttttatc ggggcggaaa acttccccgt aaaagcgtta
     1081 tgttgacatt tgatgatgga taccttgaca actggtttca ggtatatcct ttattaaacg
     1141 agtttaatct caaggcacat atttttttaa ttaccagttt tattggaaat ggtccggttc
     1201 gtcactctcc tggaaaggaa tattctcatc gcgattgcga acatcaaata gcaaccggga
     1261 atgctgataa tgttatgctt cgctggtctg aagtgaacga aatgttgcaa tctggtctgg
     1321 tcgaatttca tgttcataca catactcata cacgttggga taaaaagttc acttcacgag
     1381 aagaacagtg taaacatctt cggcaggact tattgagcgg gagagaatat ttaaaaaaaa
     1441 tgacaggaaa atgcagtaaa catttgtgtt ggccggaagg ttattataac aaggattata
     1501 ttcagatcgc tgaagaactt ggattccatt atttatatac aacagaaaga agaatgaatg
     1561 ctcctgctaa aggaactaca cggataggaa gaataagcac caaggaacgg gaaaattgcg
     1621 tatggctgaa aagacgactt ttttattata caacaccgtt tttttcatcg ttgctggccc
     1681 ttcataaggg gccaagatta ccggatgact aataatgaat atactattta cggaatcatc
     1741 accgaatata ggtggtcagg aattacaggc tgttgctcaa atgaaggccc tgaagaaaat
     1801 ggggcattca gttctgcttg tctgcaggga gaacagcaaa attgcttttg aagccagtaa
     1861 gtttggaatt gatatcacat tcgcgttatt tcgaaacagt cttcacattc ctactgtatg
     1921 gagattactc ggaatagttc atagttttca gcccgatgca atcgtttgtc acagtggaca
     1981 tgatagtaat attgttggtt tggtacggtt ttttacctgg aaacatccat tcagaattat
     2041 caggcaaaag acatatttga cacgaaaaac aaaatttttt tcaataaatc atttttgcga
     2101 tgaggtgatt gttcccggaa caaatatgaa gacacatctg gagcaggaag gatgtcgaac
     2161 tcgggttact gttgtgcctc ctggctttga tttccaggaa ttatacgttg attcccgaaa
     2221 cagtttgcct ccgagtgttc tttcctggct ggcgtcccga agaggatgcc ccgtaattgc
     2281 tcaggtagga atgttgcgcc cggaaaaagg gcacgaattt
//
LOCUS       DADMFL010000254         2300 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.262, whole genome shotgun sequence.
ACCESSION   DADMFL010000254 DADMFL010000000
VERSION     DADMFL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.262"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..784)
                     /locus_tag="JNZ27_004209"
     CDS             complement(<1..784)
                     /locus_tag="JNZ27_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8666284.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="JNZ27_004210"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="JNZ27_004210"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY8666285.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..>2300)
                     /locus_tag="JNZ27_004211"
     CDS             complement(2137..>2300)
                     /locus_tag="JNZ27_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY8666286.1"
                     /translation="ETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFGWS
                     ANTSTRGRR"
BASE COUNT          562 a          589 c          577 g          572 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagccaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactcttt
     2281 cttaatgctg ttggtttcgg
//
LOCUS       DADMFL010000255         2254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.263, whole genome shotgun sequence.
ACCESSION   DADMFL010000255 DADMFL010000000
VERSION     DADMFL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.263"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..135)
                     /locus_tag="JNZ27_004212"
     CDS             complement(<1..135)
                     /locus_tag="JNZ27_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY8666287.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     V"
     gene            complement(196..797)
                     /locus_tag="JNZ27_004213"
                     /pseudo
     CDS             complement(196..797)
                     /locus_tag="JNZ27_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(734..1477)
                     /locus_tag="JNZ27_004214"
     CDS             complement(734..1477)
                     /locus_tag="JNZ27_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666288.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            complement(1482..2180)
                     /locus_tag="JNZ27_004215"
     CDS             complement(1482..2180)
                     /locus_tag="JNZ27_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY8666289.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(2180..>2254)
                     /locus_tag="JNZ27_004216"
     CDS             complement(2180..>2254)
                     /locus_tag="JNZ27_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666290.1"
                     /translation="CRKWSASVGALWVTVTADFEQVVN"
BASE COUNT          491 a          759 c          532 g          472 t
ORIGIN      
        1 cacgcttttt aatccatcca ccggaccgtc aaccggccct tcgctgatgg catcaatcac
       61 actcagcaac tgcgtggatt tcaggttgtc cttcgcttcg cgcggggtat gccccttact
      121 gctgccttta cccattcctc acgctccata aacgacaaaa ccgcccgcag gcggtttcac
      181 atgaaacatt ttgcatcagc gaccaatcac cacaacctga ccaccatccc cttcgtctgc
      241 cgtgctgatc tcctgagata ccacccgcga ccccacgcgc atttcaccgt acagaacagg
      301 cagaacattg ccctgggcaa ccatgttatc cagtgaggag aaacaggtgt tctgtttgcc
      361 gttatccgtt gtctgtgtac ggggagttct ggctttcggt gccagcatct gtgcaacacc
      421 gccaagcgtc atactggcac cgagagaaaa cagcagatta ctcgccataa ttcctacccc
      481 ggcatccata tagcaaccgc cataacagct gcccccagca ccgcctgaaa cacaccgcca
      541 cttttagctc ccaccagacg cggtactatg tggatcactg caccatttgc cagcggctca
      601 ttaagacggg cagacaattc ggtttcacct gcatcacgcc cggcaatgcg cacctgatac
      661 cagccgtcac tcagtttctg acgaaacgcc gggagctgtg tggccagtgc gcggatggct
      721 tcagcccccg ttttcacacg aaggtcgatg cggcggccaa atcgttgcaa atccccgtaa
      781 aggcagatgc gtgccattcc cggtgacgcc agagggagtg tgtgcgtcgc tgccatttgt
      841 cggtatacct ctctcgttta ctcagttgtt caggaatatg gtgcagcagc tcaccatcgc
      901 cgcagtaaat ggcggcatga ttcggcaccg atgaaccaaa acagcacagc agcacatcgc
      961 ccggttgtgc tgatgacaac ggcacctgat acagccctgt ggcctccaga ttatccagat
     1021 agagattctg accgtgacgc caccagtcat ccccgcggtg aaaatccggc atctcaatcc
     1081 ccgccagatg ataagcatcc cggaacagcg tgtaacagtc cgtcaccccg tgctcaaagc
     1141 gccgcccggt gagatgcggc acacagcgga acttgtgaat cgccccccgg cagaccagcc
     1201 accacggcaa atcactctgc acctgcagcc gccggtcagc ctcactcagc cagggcagac
     1261 caccgggatg actgtggacc agtgccacaa tctcaccctg catttgtgcc cgcagccagt
     1321 cctccggagc catccggaac cgttcctccg gctcaccgga gatattctcg ctgggaagat
     1381 acctgtctcc ctccggcgtt ctcaccacga atccacacga ctccgctggc gcacatcgcc
     1441 gggcgtgcgc cagaatcgct gattctgtct gtgtcatgga tttactgaga aagtttattg
     1501 atggaaagga aaccgccgaa attaccggta ttgttgcgca actcacaccc gcgcatacac
     1561 ttgctgcatt tgtccttccg gatatccgtc gttggtttgt cgaactcgtc agccactgcc
     1621 ctgcccgtgt aaccacactc atcagaacgg taagtccaca tacaggtatt cgccagcatg
     1681 atacgccccg ggaaaaccgc gccatccgtc tccgttggtg tggccagcac aaaagaggca
     1741 ctgaccgcgc tcagttcgct gcactgctcg atgcgccagc ggctgatcac ctcctgttcc
     1801 ggatcggcgt cgctgtttcc gttgacgaag ttcaccgcat ccagaaaacg ggcgtaaacc
     1861 ttacgccgga ccaccgttcc gccgaccaga ctctgcagat cttccgccat cccggtgacc
     1921 atgccgtgca ggttagagac cgtcagtgtc ggacgggcag cacagcccct gccgttcagt
     1981 tcaaaccccg ccccctgaat ggggtatgcc tgatactgcc gcccctgcca ggtgaccggc
     2041 tcaccttttt cgttctgctc attacagaaa aaataacgct caccaccgac ctctgtcaga
     2101 tcgatttccc agagctccac ccgggctgac tgctccgcac gggtgcattc attcagtgtt
     2161 tcctgccgga tatcctgcat cagttcacca cctgttcaaa atcggccgtt acagtcaccc
     2221 ataacgcccc cacgcttgcc gaccatttac gaca
//
LOCUS       DADMFL010000256         2185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.264, whole genome shotgun sequence.
ACCESSION   DADMFL010000256 DADMFL010000000
VERSION     DADMFL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.264"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(219..383)
                     /locus_tag="JNZ27_004217"
     CDS             complement(219..383)
                     /locus_tag="JNZ27_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666291.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1284..1568
                     /locus_tag="JNZ27_004218"
     CDS             1284..1568
                     /locus_tag="JNZ27_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8666292.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1556..2041
                     /locus_tag="JNZ27_004219"
     CDS             1556..2041
                     /locus_tag="JNZ27_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8666293.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          566 a          461 c          619 g          539 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 tgcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaaggaac tgtcgatgaa gtgtcgaaag gaacctctag gcgatgtcga
      481 aaatgtgcta tctacgcagc gcatgacgag tccagcagat atctggtact ggctgggcgg
      541 catccggcat ataggcagca tgaacgcaac acaagctctc gtacggaacg tgggaacctg
      601 tcgtctcgat gctaagggag cggcgcggag cagaggcact gcaagcgcct gagtaccgat
      661 gcgagacaca ggggcagatg gcttcgtagt agtgaggaag tgcctgtaat gggcatggag
      721 cgaagggggt cagtcgttgg ggctggttta tcggtcaacc ggatagccgg gaggagccca
      781 tgagtcagcc aaagccgttc gaggtctcta agtatgcggt ttggaaagca taccagcggg
      841 tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc agtcgaggcg tttgaggtta
      901 ccaatcgaag ctatacccgg taatggcgca ggttgacctg cacttggcca agtggattgc
      961 ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca ttcgagtggt tactgcgaat
     1021 tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta gcttacgata cttgaatggt
     1081 tcaacgacaa gagccgtatg acgcgagagt gtcacgtacg gttccgtggg agcgtagggg
     1141 ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg attacgtcca cagaggtaaa
     1201 tgccgctgac gcggcatctg taatctgtta ctttgtgtat atcatttgct atatattgtc
     1261 aatatgctat tgttgtaaag caaatgatat acggaggctt tatgaaatca ggtgttcagc
     1321 tcaatctcag agcaagagaa tcacagcgga tactcattga tgctgctgca gaaattcttc
     1381 ataagtcgcg tacagatttc attctggaaa tggcctgtaa agctgctgaa gatgttattc
     1441 tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga agagttcatt gaaatgcttg
     1501 atgctccggt tgcagatgat cctgccatcg aaaaattgct ggcaaggaaa cctcaatggg
     1561 atgtgtaact gcaccagaac ctttatcctc atttcatcag gtcgctgaat ttgtcagcgg
     1621 tgaagctgta cttgatgact ggttaaagca gaaggggcta aaaaatcagg caatcggagc
     1681 cacccggact tttgttgtat gcagaaaagg tacgcaacaa atagtcggtt tttactcact
     1741 ggccactggt agtatcaatc atacagaagc tacaggtaac cttcggcgca atatgccaga
     1801 ccccattcct gtaattatac ttgcacgcct ggctgtagat gtgtcatttc gaggaaaagg
     1861 gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt tacagggttg ccgaaaatat
     1921 cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac gcaaaacagt tttatattca
     1981 tcatggtttt aagccatcaa aaactcaggt gcaaacattg tttcttaaac tccctcaata
     2041 gggagtaatg ccgatcagtt aaggatcagt tgaccgatcc agtggctgtg taagaatccg
     2101 gaaacgctca ctcgtttccg gatttttctg tgcacagtaa aagaccacct cctgaggagg
     2161 tggcttttca gaatgccccc tataa
//
LOCUS       DADMFL010000257         2161 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.265, whole genome shotgun sequence.
ACCESSION   DADMFL010000257 DADMFL010000000
VERSION     DADMFL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.265"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            83..787
                     /locus_tag="JNZ27_004220"
     CDS             83..787
                     /locus_tag="JNZ27_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8666294.1"
                     /translation="MPLQVLGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRH
                     PGAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCF
                     AWLADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQ
                     QPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGC
                     LISAMTALLMKDNQRS"
     gene            889..>2161
                     /gene="amn"
                     /locus_tag="JNZ27_004221"
     CDS             889..>2161
                     /gene="amn"
                     /locus_tag="JNZ27_004221"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8666295.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTMVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLL"
BASE COUNT          505 a          531 c          516 g          609 t
ORIGIN      
        1 gctaaaattt ctttttttca ttggagcacc tgtgttgttc tgaggtgagc atatcacctc
       61 tgttcaggtg gccaaattca gtgtgccact tcaggtactg ggagcattgt gggtgcgcaa
      121 tggcatggag gagtccgcgg aatttgaaca acagcaacat aatcaagcgg ccgcgaaaaa
      181 acgcatcccg gttatcgaag ctctgttacg acatcccggt gctttcctga agattattgc
      241 actacgactg tgcgagttgc tgacgatgta cattgttact gcctttgcac ttaattattc
      301 aacccagaat atggggttac cgcgcgaact tttccttaat attggtttgc tgttaggtgg
      361 attaagctgc ctgacaattc cctgttttgc ctggcttgcc gatcgttttg gtcggcgcag
      421 ggtttatatc acaggcgcgt tgatcggaac gttgagcgca tttcctttct ttatggcgct
      481 tgaagcacaa tctattttct ggatagtttt cttctccata atgctggcaa acattgcgca
      541 tgacatggtg gtgtgtgtgc aacaaccgat gtttaccgaa atgtttggtg ccagttatcg
      601 ctacagtggt gctggagtcg gttatcaggt tgccagtgtg gttggcggtg gatttacacc
      661 ttttattgcc gctgcactca tcacttactt tgccgggaac tggcatagca tcgccattta
      721 tttgctggct ggatgcctga tttctgcaat gaccgcttta ttgatgaaag acaatcaacg
      781 ctcgtgatag cctgccgaag atcatccgat cttcgcctta tacttttgtt tcacattcct
      841 gtgacatact atcggatgtg cggtaattgt atggaacagg agacacacat gaataataag
      901 ggctccggtc tgaccccagc tcaggcactg gataaactcg acgcgctgta tgagcaatct
      961 gtagttgctt tacgcaacgc cattggcaaa tatattacaa ttggcgaatt acctgatgaa
     1021 aacgcccgta aacaaggtct ttttgtctat ccatcactga ccgtaacctg ggacggtagc
     1081 acaacaaatc cccccaaaac gcgcgcattt ggtcgtttta ctcacgcagg cagctacacc
     1141 accacgatta ctcgccctac tctctttcgt tcgtatctga atgagcaact aacgttgctt
     1201 tatcaggatt atggtgcgca tatctctgtg caaccctcgc aacatgaaat cccttatcct
     1261 tatgtcatcg atggctctga attgacactt gatcgctcaa tgagcgctga tttaactcgc
     1321 tacttcccga caacagaact ggcgcaaatc ggcgatgaaa ctgcagacgg catttatcat
     1381 ccaacggaat tctccccgct atcgcatttt gatgcgcgcc gcgtcgattt ttccctcgca
     1441 cggttgcgcc attataccgg tacgccagta gaacattttc aaccgttcgt cttgtttacc
     1501 aactacacac gttatgtgga tgaattcgtt cgttggggat gcagccagat cctcgatcct
     1561 gatagtccct acattgccct ttcttgtgct ggcgggaact ggatcactgc cgaaaccgaa
     1621 gcgccagaag aagccatttc cgaccttgca tggaaaaaac accagatgcc agcatggcat
     1681 ttaattaccg ctgatggtca gggtattact ctggtgaata ttggcgtggg accgtccaat
     1741 gctaaaacca tctgcgatca tctggcagtg ctacgcccgg atgtctggtt gatgattggt
     1801 cactgtggcg gattacgtga aagccaggcc attggagatt atgtccttgc acacgcttat
     1861 ttacgcgatg accacgttct tgatgcggtt ctgccgcccg atatacctat tccgagcatt
     1921 gctgaagtgc aacgtgcgct ttatgacgca accaagttag tcagcggcag gcctggtgag
     1981 gaagtcaaac agcggctgcg tacaggtact atggtaacca cagatgacag gaactgggag
     2041 ttacgttact cagcttctgc acttcgtttt aacttaagcc gggccgtagc aattgatatg
     2101 gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg tgccatacgg gacactactg
     2161 t
//
LOCUS       DADMFL010000258         2151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.266, whole genome shotgun sequence.
ACCESSION   DADMFL010000258 DADMFL010000000
VERSION     DADMFL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.266"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNZ27_004222"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNZ27_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY8666296.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JNZ27_004223"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JNZ27_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JNZ27_004224"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JNZ27_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JNZ27_004225"
     CDS             1905..>2151
                     /locus_tag="JNZ27_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666297.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          633 a          394 c          491 g          633 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADMFL010000259         2141 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.267, whole genome shotgun sequence.
ACCESSION   DADMFL010000259 DADMFL010000000
VERSION     DADMFL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.267"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..211)
                     /locus_tag="JNZ27_004226"
                     /pseudo
     CDS             complement(1..211)
                     /locus_tag="JNZ27_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            510..763
                     /locus_tag="JNZ27_004227"
                     /pseudo
     CDS             510..763
                     /locus_tag="JNZ27_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            920..>2141
                     /locus_tag="JNZ27_004228"
     CDS             920..>2141
                     /locus_tag="JNZ27_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8666298.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKYIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAA"
BASE COUNT          557 a          439 c          491 g          654 t
ORIGIN      
        1 aacctcgatc ggtgtggtgg attagcccgg caggtgggcg ctggctcctg agcgccataa
       61 acagggcttt acccgtcaac tctttcgtca tgcgctctcc catggcgtaa ccgacaattt
      121 cgcacgtata aacatctttg acgccagcga gatacagcca tccttcccgg gtggcaacat
      181 acgttatatc tgcgacccag acctgattag gggggttggg gagcaatgga acagtaaacg
      241 ccgttaagaa gtcaatttaa atagccaaaa attaactagc caatttacat tgcaggttgt
      301 aatttttaac cacctttttt acgatagcca aattacctat acccatcccg gccctgccaa
      361 gccatacccg ctatcggcga tgaaaatgct tttgtgcgcg gtcggagtgg tcccgacgag
      421 ggttttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag
      481 cgtgtatgag agacgtacag tggttttgtc cgctcacacc agtacataca ggtacggaaa
      541 taaaaccagt cgaaatgccg gtaacgacga ttactcctgt ctctgatgcc ggtggactac
      601 aagattttat ttactggcgt cctgatgcgg acaggactgg tatggaacct gtttatgtga
      661 tacttaatga tcctctggat tccgggagat ttacgcgtaa acaactaaat aaaaatatct
      721 taaacatgca agtaattttg gtattctcga tacaagaata aatagtagta actactatag
      781 ttgcaaataa tgttaaaaac atttctatga tcatctttac atttttcatg attgacaatt
      841 gtttttggtt aaaaaaagac aactaatgag tgtgataatg caatactttg agtgattata
      901 taatgataag taaacaggtt tgcgacaatt ggttatcagt attacagaag gattaaatat
      961 gtcattattc acagaaccta aggaaattga aagattaccc tctgaagaga ttgaacgatt
     1021 atatcccgtc ctgcgataca gggtctttat cagcattttc ctcggatata tggggtatta
     1081 ttttgtccga aacaccacca gtgtgttatc cggtgtgctg cacatgtctg caaccgagat
     1141 cggcattatc tcctgtgccg gttttttatc ctatggtata tcaaagtttg tttccggact
     1201 tatttctgac agaagtaatt caaaagtatt tttgtctctt ggtttattcc tgtccggact
     1261 ggtaaatttt ctgataggat atattcccgg gatcatcaca tccgttacgt tattttcaac
     1321 aatgtattta cttaacggct ggatccaagg tatgggatat cctcctggtg ccaaaacact
     1381 ggtgttctgg tatgaacata gggaaaggat cacctgggcc acactgtgga atttatcgca
     1441 caatgtcggg ggagctctgg cccccgttct gattggtttc agttttggtt ttttcggcga
     1501 cagtgctctg gagcatgccc gggccgcatt tatcttccca ggagtactgt gtatggcaat
     1561 gagtgtgctg atttacttta ttcaggtgga cagacctgta tcagtggggc ttcctcccat
     1621 tgaagaatgg aaaggtaacg tcgtttcaca cccggctaaa ggccgtgaac aaggtccgag
     1681 actctccatt cctgatatca tcagaaaata tatcatccga aataataaac tcatttactg
     1741 ctgtatctat ggttcgtttg tttatattct gagatacggt attgtttcct gggctcctaa
     1801 gtttttaagt gacagtctgg atgtcggtgg taaggatatg ggaaaactgg catcaatggg
     1861 ggggttttct gtttttgaaa tcggtggtgt ggctggtatg cttcttgccg gatacctgtc
     1921 tgtcagactg ttcagaaact caaaaccatt aacaaatact ttatttctgg cacttaccat
     1981 tattttatta atcgcgtact ggtacgtgcc atcaggcaat gaatatctct ggctaaacta
     2041 tacaatcctt attctgttgg ggctggcagt gtatggtccg gtgatgttta ttggtcttta
     2101 ttcaatggag cttgttccta aagaagctgc cggagccgca t
//
LOCUS       DADMFL010000260         2138 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.268, whole genome shotgun sequence.
ACCESSION   DADMFL010000260 DADMFL010000000
VERSION     DADMFL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.268"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..161
                     /locus_tag="JNZ27_004229"
     CDS             <1..161
                     /locus_tag="JNZ27_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8666299.1"
                     /translation="CRYEKASIILTSNKGVADWGEMFGDHVLATAILDRLLHAEYQRR
                     ELPVKRET"
     gene            655..1233
                     /locus_tag="JNZ27_004230"
     CDS             655..1233
                     /locus_tag="JNZ27_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666300.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="JNZ27_004231"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="JNZ27_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2138
                     /locus_tag="JNZ27_004232"
                     /pseudo
     CDS             1584..2138
                     /locus_tag="JNZ27_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          687 a          391 c          528 g          532 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctacggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgcggc
     1921 caacagtgac gtccccatga tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg
     1981 tatcctgaac gccatgcgcc atggcgtatc aaacggaaat gcggaagcac tgaacagcaa
     2041 gatcaggctg ctgagaataa aagccaaggg ctaccgcaac cgggagcgct ttaagctggg
     2101 agtgatgttc cactatggga aactgaatat ggcgttct
//
LOCUS       DADMFL010000261         2136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.269, whole genome shotgun sequence.
ACCESSION   DADMFL010000261 DADMFL010000000
VERSION     DADMFL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.269"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            14..487
                     /locus_tag="JNZ27_004233"
     CDS             14..487
                     /locus_tag="JNZ27_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8666301.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNZ27_004234"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNZ27_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY8666302.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="JNZ27_004235"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="JNZ27_004235"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY8666303.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DADMFL010000262         2129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.270, whole genome shotgun sequence.
ACCESSION   DADMFL010000262 DADMFL010000000
VERSION     DADMFL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.270"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..835
                     /locus_tag="JNZ27_004236"
                     /pseudo
     CDS             1..835
                     /locus_tag="JNZ27_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1274..2111
                     /locus_tag="JNZ27_004237"
                     /pseudo
     CDS             1274..2111
                     /locus_tag="JNZ27_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          524 a          516 c          642 g          447 t
ORIGIN      
        1 aaatggtggt gtccgctatt gccagtacac ctcagttctg ggccttgccc gtagcagtta
       61 tttttatcac cgggctgcac tgaaagccgg tgataaatac gccacgatac gtaagatgct
      121 gacagatatc tttaacagta attaccggtg ttatggctat cgtcgcctgc atgcgatgct
      181 caggcatgag gggggtcggc tatcagaaaa ggttgtacgc agacttatgg tggaggaaca
      241 gcttgtcgtc agtcgtaacc gtcgtcgtcg ctacagctca tattgcggag aaatcggacc
      301 ggctccggat aaccttatcg ccagagattt taaggcggag caacctaatc agaaatagct
      361 gacagatatc acggagttcc agctccctgc aggtaaagtc tggctgtcac cagtggtgga
      421 ctgcttcgat ggaaaagttg tgagctggtc tctcagtaca cgccctgatg ctgaactggt
      481 caacactatg ctggatagcg ctgtcgaaac gttaaatgct ggcgaacgac cggtgataca
      541 cagtgacaga ggtgggcatt atcgctggcc aggctggctg gaaagagtga atgcagcagg
      601 tcttattcgc tcaatgtccc gtaaaggatg ttcacctgat aatgctgcat gcgaaggctt
      661 tttcggcaga ctgaaaacgg aaatgtatta tgggcgtaaa tggtcgggca tcacgccaga
      721 aaagttcatg cagcaagtgg gtgcttacat cagatggtat aacgagcggc gtataaaatt
      781 atcgctgggt gccgtcagcc ccaaaatgta ccgccaacaa tgcgggctgg aatgataaag
      841 cagtccagga aatcgtccgc atcccccctt caccatttgg attgtccgct ccacccaaca
      901 tgttgtttcc ttaaggttcc tacaccacaa aggaaactct catgctgcgc agtggatggc
      961 tgacgttgcc gacaggcggg aacttcgcca gttcaggcag acaccggaac agcgcttcac
     1021 gcaggaacag gagcatctgc agccgctgct gggtacggac ttcgatatcc gtcatgtctc
     1081 ctgggatggc tatatcgagg ttggcgggaa ccgttacagt gtgccggaaa gcctgtgtgg
     1141 tcagctggta tcaatacgga tctcgctgga tgatgaactg cggatctaca gtaatgagca
     1201 acaggtagca tcgcaccgtc tctgttcggc tgcatacggc tggcagaccg tgccggagca
     1261 tcacgctcca ctctggcagc aggcttcgga gcgcatggcc gaacggctgg gggagataca
     1321 gaaacgggta atcactgtct gtgaccggga agcggatatc tggcattacc tgtattataa
     1381 agtcagccac ggacagcgtg gtgcgtgctg cacagaatcg ccggctggaa gaggcacccg
     1441 gcaagctctt cgaactgccg gaagtcctgg caaccgcagg aagccacacg ctgaatgtga
     1501 tgcaaaaagg cgggcgggca gcccgtcagg cccggatgtt catcagttac agcgaagtca
     1561 gcataaaaaa tcccgacaac agcggccagg cgctcccgct cacgtatgtc tgttgccggg
     1621 agcaggcaga ggacggtgcc tgctggcatc tgctgacgtc aggaaaagcg gcgagtgccg
     1681 ccgatgcacg acgtattgtc agccattacg agcgacgctg gctgacagag gaataccaca
     1741 aggcgtggaa aagtggtggt acatggaatc gctgcgaatg cagacccggg ataaccttga
     1801 gcgcatggtg gttatccagg cgtttatagc ggtacgggtg ctgggcctgc ggcagggagg
     1861 tgtcagtgaa gaaacacaaa atgacagctg tgagaaaata ctgacgccga cagagtggaa
     1921 actactgtgg gttaagctgg aaggaaagcc gcttccggtg caggcaccga ccctgaaatg
     1981 ggcctggggc gatggcatga cagcaaacgc acaggtcgtc ccggttgggg cgtcatgtgg
     2041 gatggctggt cagacttcag gatatggttg agggttacct ggtgatgaag tctcttgatc
     2101 aggagctctg atcaagagac agcagctta
//
LOCUS       DADMFL010000263         2127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.271, whole genome shotgun sequence.
ACCESSION   DADMFL010000263 DADMFL010000000
VERSION     DADMFL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.271"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(246..479)
                     /locus_tag="JNZ27_004238"
                     /pseudo
     CDS             complement(246..479)
                     /locus_tag="JNZ27_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..2097)
                     /locus_tag="JNZ27_004239"
                     /pseudo
     CDS             complement(575..2097)
                     /locus_tag="JNZ27_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          637 a          466 c          450 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatagt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagtggatgt tcaaaatagc
     2041 gacaccggta ataaacaagc atatcgcaac gaggatgggt atgagtgtat tgcgcatttg
     2101 cttgtcccgg ccaaagggtt taataca
//
LOCUS       DADMFL010000264         2104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.272, whole genome shotgun sequence.
ACCESSION   DADMFL010000264 DADMFL010000000
VERSION     DADMFL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.272"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="JNZ27_004240"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="JNZ27_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY8666304.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTVYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /locus_tag="JNZ27_004241"
     CDS             1351..2016
                     /locus_tag="JNZ27_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8666305.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVAMAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          579 a          446 c          564 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggagatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgtttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga caggaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtgatgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatta
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccatggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg cgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgtccgg
     2101 gcgc
//
LOCUS       DADMFL010000265         2070 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.273, whole genome shotgun sequence.
ACCESSION   DADMFL010000265 DADMFL010000000
VERSION     DADMFL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.273"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(88..1500)
                     /gene="pykF"
                     /locus_tag="JNZ27_004242"
     CDS             complement(88..1500)
                     /gene="pykF"
                     /locus_tag="JNZ27_004242"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY8666306.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          526 a          458 c          502 g          584 t
ORIGIN      
        1 gattttttat tgcttctggt tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga
       61 tatacaaatt aattcacaaa agcaatatta caggacgtga acagatgcgg tgttagtagt
      121 gccgctcggt accagtgcac cagaaaccat aactacgacg tcacctttgt gtgccagacc
      181 gctctgcaga gccagttctt tacccagacg gtagaaatca tcagtagaag tgatctcttt
      241 aaccagctgc ggcacaacgc ctttgctcag taccaactga tgagccgttt tttcgttggt
      301 ggtcagtgcc aggatggtgg catccgggaa gtatttacgt actgcgcgag cagatttacc
      361 gccctgggta gcaaccacga tcagcggagc atccagtttt tcagcagttt caacggcacc
      421 acggcatacc gcttcggtga tgcgcagttt acggttgtca ttgttgaact cgagacggct
      481 gttcatcacg cggtcggtac gttcgcagat ggtcgccatg atagaaaccg cttccagcgg
      541 gtatttacct tttgcggatt caccagacag catcactgcg tcagtaccgt cgaggatggc
      601 gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg tttttgatca tggaatccag
      661 catctgggtc gcagtgataa cgactttacg tgcacggata catttttcga tcatcatctt
      721 ctgggcgaag ataacttctt ctaccgggat ttctacaccc aggtcgccac gcgcaaccat
      781 gatgccgtca gaggcttcga ggatttcgtc gaagttgttg aggccttcct ggttttcgat
      841 tttggagatg atgtggatgt tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat
      901 aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag tctacgcctt tttcgcaacc
      961 aaagatcagg tcctgtttgt ctttttcagc cagtgctggc agagcaatgg aaacgccagg
     1021 caggttcaca cctttgtttt cgcccaggtc accgttgttc agcactttac agataacttt
     1081 gttaccttca atggcggtaa cttccatacc gatcagacca tcgtcaacca gtacggtgtt
     1141 gccaacagac aggtcagtag tgaaaccttc atacgttacc gcaaccattt cgctgttgcc
     1201 gataacagat ttatcagtgg tgaaagtaaa ggtctgacca gctttcagag aaacatcgtt
     1261 accgccttcc agtttcatgg tgcggatttc cggacctttg gtatcaagca ggatagcggc
     1321 ggttttacca gttttgctca tcacgttgcg cagattctga atgcgctgac cgtgttctgc
     1381 atagtcacca tgagagaagt tcagacgcat aacgttcatg ccagcgtcca gcattttagc
     1441 taacatctct tcagattcgg ttttcggtcc gatggtgcaa acaattttgg tctttttcat
     1501 gacagtctta gtctttaagt tgagaaggat gggagaaact tgctttctgg gcgcactgcc
     1561 ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg
     1621 aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca acgtgagtgc ctgtttttga
     1681 gtcgttgagt tctacagtcc aaagtgctga aaccattcaa gagtcaattg gcgcgcatta
     1741 tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt tcaaaaaata tacaacttgt
     1801 tagacaataa attgttatca aagatttaac gttccaggga aaaggttacg caaaaacttc
     1861 gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga ggataagtga aagttcatcc
     1921 ggtgtataaa tatatcatcg ctcaattgct acagttaaaa taatttatcg cgttattttt
     1981 ctgattttgt ttgatttttg tttaattggt tagattgata tatgctgata gttggcatga
     2041 taaataggta taaagaatgg gaaatcgggt
//
LOCUS       DADMFL010000266         2065 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.274, whole genome shotgun sequence.
ACCESSION   DADMFL010000266 DADMFL010000000
VERSION     DADMFL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.274"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..169
                     /locus_tag="JNZ27_004243"
     CDS             <1..169
                     /locus_tag="JNZ27_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY8666307.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            154..792
                     /locus_tag="JNZ27_004244"
     CDS             154..792
                     /locus_tag="JNZ27_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY8666308.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..1424
                     /locus_tag="JNZ27_004245"
     CDS             1020..1424
                     /locus_tag="JNZ27_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666309.1"
                     /translation="MKKQIFINNKPPVVPYSGTHVKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGGR"
     gene            1435..1909
                     /locus_tag="JNZ27_004246"
                     /pseudo
     CDS             1435..1909
                     /locus_tag="JNZ27_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          625 a          369 c          485 g          586 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttt tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg attctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tacaggaaaa cgcctgaggg gctggttgcc tgtagcctgt
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata aaatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 tgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagtgggg ggggggggag atgaacccta tgcaatgtcc
     1441 tggttaatat ctcagtgtgc tcatcagtgc actgataata aaaagacgga gacagatgcc
     1501 atttacgata aagttcggag ttcttatttg ctttcatgta ttctgaaaaa aaaacaaaaa
     1561 tgttgggctt attcttcatg caccatcatt tgtttctgtg tctgaaaaaa tcgccagaat
     1621 tgtgatggca aattactccc gtaactggag taattccgag ttggccagtg cggttttaat
     1681 gagtgaatct tctttaaaaa gaagaatgta caaagaggtc ggttctataa gtacatttgt
     1741 acataaaata aagcttacag aagctatcag gaaattacga aggacaaata ctcccatttc
     1801 tgtcatatca tctgagttag gttacagttc accatcgtat ttttctaaag ttttctttaa
     1861 atatttaaag acataccctc aaaacataag gaagaaaaat ggtcggtaat gtcaccaatt
     1921 aggaggactt gcagagccgc ttttttacga tactgagatc aaacagacat aatctatgcg
     1981 gagatttaca gcagggcatg gttcacaaat ctgacagcga tgaattatcg gcactgcggg
     2041 cagaaaatgc tcgaatcatc aagcc
//
LOCUS       DADMFL010000267         2063 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.275, whole genome shotgun sequence.
ACCESSION   DADMFL010000267 DADMFL010000000
VERSION     DADMFL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.275"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..124
                     /locus_tag="JNZ27_004247"
     CDS             <1..124
                     /locus_tag="JNZ27_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666310.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="JNZ27_004248"
     CDS             complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="JNZ27_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY8666311.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          647 a          366 c          337 g          713 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt
      181 tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc
      241 agattcaggg cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc
      301 gctccatttt ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta
      421 ttaataaaat attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca
      481 tcatacagct cacaatacat attgttgttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa
      601 taagcaagag cttgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa
      661 ttggatgctt taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta
      721 tctgtgctta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc ttttgtatta
      901 aatattggca catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattag caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc
     1201 tcagagaatg tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta
     1261 atattaagag tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa
     1321 ttaataataa tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt
     1381 atttcagaaa gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag
     1501 aacattttac tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat
     1621 atgttttttt gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca
     1681 atattgttct gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca
     1741 ttatgtgcgt ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg
     1801 tatgtattat aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga
     1861 gtttaatcac acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat
     1921 atatatcttg cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg
     1981 caaaacaggg catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa
     2041 gaatcatggg cgctcaacaa aaa
//
LOCUS       DADMFL010000268         2059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.276, whole genome shotgun sequence.
ACCESSION   DADMFL010000268 DADMFL010000000
VERSION     DADMFL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.276"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..497)
                     /locus_tag="JNZ27_004249"
                     /pseudo
     CDS             complement(1..497)
                     /locus_tag="JNZ27_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308290.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(673..1717)
                     /locus_tag="JNZ27_004250"
                     /pseudo
     CDS             complement(673..1717)
                     /locus_tag="JNZ27_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            complement(1963..>2059)
                     /locus_tag="JNZ27_004251"
     CDS             complement(1963..>2059)
                     /locus_tag="JNZ27_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8666312.1"
                     /translation="ERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
BASE COUNT          505 a          564 c          491 g          499 t
ORIGIN      
        1 ccttcgataa aagccatccc tgtaaacgtt cgtcgcgggt tgccacgttc aatctggtga
       61 tggaacattc gccgctgtga tttcagctcc gcgctatttt cctattgctg ctgatccagc
      121 ttccaggcaa tcagcaatcg cgccagccgt ttgttagcat gctggctgcg ctctgactga
      181 accttcacgc taataccgga tgccaaatgc gtggcgcgta ccgccgagtc ggttttattg
      241 acatgttgac cgcccggccc cgacgaacgc agcgtctcat accggatcgc atcggattgc
      301 tcctgctcat cagcggtaaa acgcccaatg cccagaaacc agtttttacg cccgtgatgt
      361 gggcgatacg ggctcgggca aatctactga atagtgccac accagctttc gcttaacgcc
      421 catgcgttgt cgccatccag agaaatcagc gccgaacgca gcgtgtcaga gtagcggcct
      481 gtttctgttt ccagcaccgt taccgcgacg tcttgtcggg cagcttcttt aattagcctg
      541 tccagcgcct ttttcactgc gagacaacat tcttccggcc cctgcgctga ggagagttat
      601 agcaagatca tccttttttc cctccactgt ttttgagcgt tagcaccgga cgcagccgcg
      661 cgacaggaat aattaatccg gcctgcccag acattgcacc acactttcag ccgatttata
      721 ggcttgtggc gcttcttcga agatgagttg tttatcgcga caaattaccc gactgccaag
      781 ttcagtacgc gagagttgcg tcgctgtgta tttcgctgcc agacgccctt tacactcggt
      841 gcgcccccat ttacgcccag ccccatgcgc cagcgaatgc aatgtttcct cactcctgac
      901 gggcctgaac cagcctggag taatcaccgc gtgaaccggg aataaccacc agaccacagt
      961 catcaggcgt ggcaccttta cgatgtaaga ccccttgttg atcaccgatt cgacacgcgc
     1021 taacaaagtt atgcgccaca tccagaaccg gacttccggt ggccttaacc tgttgcatta
     1081 tgcgcaaagc gatcaggtgg cgattaaaac gcgcaaacgc cagcgcgtcg tcactgcctt
     1141 caggcaaacc atgatgcgaa aacgaggcaa tatgccgctg taaaatagac tgccctagct
     1201 ccctcgagcc gctatggacc agcagttgca gatgctgcgc atccagacct gccagtgcaa
     1261 acagttcagc gttgatgatt tgatcaactt gttgcagttc agcgaaatga ttgccaccac
     1321 cgatagagcc aagcgagctg cgccacggat gctgtgcaaa tgctgacggc aggttttcct
     1381 ccagccagct ttcttcagca acgtcatcca aagctgataa tcgcttttca aacttatcgg
     1441 cgttgtattt gcgagcgaga atatctgttt gccatagcgc catgccacag ccgatatcat
     1501 tgccgaccag tgctgggtaa aaacggcctg cggagaagaa cgctgcgcca atcgggtagc
     1561 cgcgtccggg gtgtaaatct ggcatcccca ctacgcgctg catgttgggt aaatttgccg
     1621 tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat gcgatggtaa
     1681 ataccgcatc ggataaggga cgaatataat tgcccatata ctattccgta aatagactcg
     1741 tcatacttca agttgcatgt gctgcgactt gaattattga gagtaacaat taaaaaatca
     1801 ggaaatggca gacgaatcca gggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa
     1861 gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga ataagaaaac
     1921 gcgcacatac taggcgggcg ttattttcat tgcaagctgg atttaatgtt gcggtttata
     1981 acgcatataa aaattaataa accacgctcc ccggcattca acgcgctgct gcttaactgt
     2041 ctgaaaacca taacgttca
//
LOCUS       DADMFL010000269         1948 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.277, whole genome shotgun sequence.
ACCESSION   DADMFL010000269 DADMFL010000000
VERSION     DADMFL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.277"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(821..1060)
                     /locus_tag="JNZ27_004252"
     CDS             complement(821..1060)
                     /locus_tag="JNZ27_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8666313.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1255..1613)
                     /locus_tag="JNZ27_004253"
                     /pseudo
     CDS             complement(1255..1613)
                     /locus_tag="JNZ27_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1624..1948)
                     /locus_tag="JNZ27_004254"
                     /pseudo
     CDS             complement(1624..1948)
                     /locus_tag="JNZ27_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          431 a          543 c          470 g          504 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctacttgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacttgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtctgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
     1621 atgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag tgtggacctt
     1681 tttaccccgg agaacaccgg caagccccat aaccgccatg agacgtgcca cagtgcatct
     1741 ggccactctg ataccttccc gtaacaactg atgccagact ttacgcacag cgtatacctg
     1801 atgattctca tcgtatacgc gcagtatctc tttcttcagc cagtcatcgc gctgcgcacg
     1861 ggcactgcgt ttatcatgat gatgtcgctg ttgctgacag tggtaatacg ttgacggggc
     1921 aatatgcagt tcactgcata gcggtccg
//
LOCUS       DADMFL010000270         1918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.278, whole genome shotgun sequence.
ACCESSION   DADMFL010000270 DADMFL010000000
VERSION     DADMFL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.278"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(346..1389)
                     /locus_tag="JNZ27_004255"
     CDS             complement(346..1389)
                     /locus_tag="JNZ27_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY8666314.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            complement(1801..>1918)
                     /locus_tag="JNZ27_004256"
     CDS             complement(1801..>1918)
                     /locus_tag="JNZ27_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8666315.1"
                     /translation="VDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES"
BASE COUNT          554 a          391 c          403 g          570 t
ORIGIN      
        1 aggactattg atttcataac agcgaataca attttttact gtttataatg gctgcctgtg
       61 ttggacgggg ataccagttc aaccgaaaac gccagaggag gggattaccc gctggcaggg
      121 taaatctgtg gtttttgatg ccagctataa cgtgtttgac gataaagtat tacgaaagaa
      181 ctttaatgag ttcatggagt aattcgttaa agtaatttac aacgctatcc ttttgcaaag
      241 gaaactttca gaaaataacc ccagggtaat cacctgggga aggaacacag tgttattatt
      301 ggataataaa ttattacttg ttttaaagtt acactaatgc aatatctact gatactggat
      361 tcttgaatgt ttcatccctg ctgcggaaaa gggcaatatc cagggccaga tatattcacc
      421 gttcatattt ctgcatttct gtacaatttc ctcagaaagt gtcttgtcaa ttcttttcca
      481 caggtgctcc tgctgtctta aaatactttc gcatatttcg aaatcatctt cctgaatcag
      541 catttcggcg catttaatga tttctgatcg ttcatgcagc acggaggaat ggttatagag
      601 ccagtttaca aacagcaatg cttccggata ttgtccccat aatggtgagt atgttgcata
      661 gtaatcttct gccgtatcca gagagccgga gaagtcatgc cacacagcaa gtggaaatgg
      721 atgcagattt ccgcacagca tttctgcgat gtaagtgttt gtggcaaagc catggcgaaa
      781 agctgtggcg gccttaacat gttccccgtt cagaaaatgc aggagaccga gttcatacca
      841 gtaaggtgaa aattcatcag catgctcttt cagtacactg aaggctcgct catgatcgcc
      901 ggtgcgtaac agctctgaac ccaacaacca gcgagccccc tgattatctt caggattgta
      961 tgccagtatt ttatcagtga gcataaccgc atcctgatgg cgctgtaagt gcacatttgc
     1021 gagaattgct gcataaagcg cgcgcagata aggccggttt tcgggatgca tccagattat
     1081 ttcaccacag aaactttccg ggatgatacg attacctgcc gccagtcctt taagagcggc
     1141 gttcagtgcc ttacgcgggg cattctgttc cagaaatgca tatgccagat gagcgtgtat
     1201 atcaataaag tccggctccc gttgagcaag gcgtcggagt ttgttgatgt aactcgtctc
     1261 gctgatttca tcagcgtcgt atgcatcaag cacttcttca aactccccca gcaatgggcc
     1321 aaaccaggta tccggaaata cgaaataacc cttgttatcg tgcacttcaa agcataatct
     1381 atcgttcatt ttctgagcct gtaaaacatg tggtgatata gtctgagagc ataaaagttg
     1441 agattctgtg tatcaagtcc tgtcttggca ccgaatgaat attgcttacg aagccccttc
     1501 atgctttcct tgagaacaat aattttatct ttatgtgaat ctcatttttt ataatgatgt
     1561 cagatgattg aaatgctaaa ttatatctga atcaactttt cctttgctaa aggtatcgaa
     1621 ataactgcaa ggctgtattt tgtaattgcc aatttaaaaa tgtcaatttg tttttaatta
     1681 tgtttttgta ataagtttga agacgcaaaa aaataatata ataacaaaaa tcctccagcc
     1741 ttgtaggcca gaggataaaa tacttaattg aggaaaacat cagggaaaag tatttctgac
     1801 ttaactttcc cggctggaga gtgaatcaat gtaatcagcg taccattgca tcatttcacg
     1861 gcggccatcg agatacaggg catgattata ggtacccctg atggaatttt tatccaca
//
LOCUS       DADMFL010000271         1915 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.279, whole genome shotgun sequence.
ACCESSION   DADMFL010000271 DADMFL010000000
VERSION     DADMFL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.279"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..721)
                     /locus_tag="JNZ27_004257"
                     /pseudo
     CDS             complement(1..721)
                     /locus_tag="JNZ27_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            754..1770
                     /locus_tag="JNZ27_004258"
     CDS             754..1770
                     /locus_tag="JNZ27_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HAY8666316.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
BASE COUNT          494 a          514 c          512 g          395 t
ORIGIN      
        1 gggggtgttt atcgaagttc accagatttt caccaggatg cacgtcatac tcttttttct
       61 ccggaggcag tgccagcatg gactgctgct cttcgagcca gcgatcgcag ggacgggcct
      121 ggattgtttc atgctttcgt tggttagcga catcgtgcag ccagcgcaga ccgtggcggt
      181 tggctgtttc aacatcgaca gtgatcccca tcgggcgcag gcgagtcatt agtgggatgt
      241 aaaaactgtt acgggtgtac tgcgccatcc gttccacctt acctttagtc tgtgccctga
      301 aggggcgaca cagtcgggga gagaagccca tctccttgcc gaactgccac agcgaaggat
      361 ggaaccggtg ctgaccggtc tgatatgcgt cacgttgcag aaccacagtt ttcatattgt
      421 catacaacac ttcgcgcggc acaccaccaa agaagcggaa cgcattacga tggcaggtct
      481 ccagcgtgtc ataacgcata ttgtcagtga attcgatgta caacattcgg ctgtatccgg
      541 gaacagcaac gaacacgtga agcggtgagc gaccattacg catagtgccc cagtcaacct
      601 gcatctgtcg tccgggttca gtttcgaacc gaacggcagg ctcctgctcc tgagaaaccg
      661 agagagaacg aatgaacgcc ctgagaatgg tcattccgcc acgatatccc tggtctctga
      721 tggaaggtgc gaacaagtcc ctgatatgag atcatgtttg tcatctggag ccatggaaca
      781 gggttcatca tgagtcatca acttaccttc gccgacagtg aattcagcag taagcgccgt
      841 cagaccagaa aagagatttt cttgtcccgc atggagcaga ttctgccatg gcaaaacatg
      901 gtggaagtca tcgagccgtt ttaccccaag gctggtaatg gccggcgacc ttatccgctg
      961 gaaaccatgc tacgcattca ctgcatgcag cattggtaca acctgagcga tggcgcgatg
     1021 gaagatgctc tgtacgaaat cgcctccatg cgtctgtttg cccggttatc cctggatagc
     1081 gccttgcctg accgcaccac catcatgaat ttccgccacc tgctggagca gcatcaactg
     1141 gcccgccaat tgttcaagac catcaatcgc tggctggccg aagcaggcgt catgatgact
     1201 caaggcacct tggtcgatgc caccatcatt gaggcaccca gctcgaccaa gaacaaagag
     1261 cagcaacgcg atccggagat gcatcagacc aagaaaggca atcagtggca ctttggcatg
     1321 aaggcccaca ttggtgtcga tgccaagagt ggcctgaccc acagcctagt caccaccgcg
     1381 gccaacgagc atgacctcaa tcagctgggt aatctgctgc atggagagga gcaatttgtc
     1441 tcagccgatg ccggctacca aggggcgcca cagcgcgagg agctggccga ggtggatgtg
     1501 gactggctga tcgccgagcg ccccggcaag gtaagaacct tgaaacagca tccacgcaag
     1561 agcaaaacgg ccatcaacat cgaatacatg aaagccagca tccgggccaa ggtggagcac
     1621 ccatttcgca tcatcaagcg acagttcggc ttcgtgaaag ccagatacaa ggggttgctg
     1681 aaaaacgata accaactggc gatgttattc acgctggcca acctgtttcg ggcggaccaa
     1741 atgatacgtc agtgggagag atctcactaa aaactgggga taacgcctta aatggcgaag
     1801 aaacggtcta aataggctga ttcaaggcat ttacgggaga aaaaatcggc tcaaacatga
     1861 agaaatgaaa tgactgagtc agccgagaag aatttccccg cttattcgca ccttc
//
LOCUS       DADMFL010000272         1906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.280, whole genome shotgun sequence.
ACCESSION   DADMFL010000272 DADMFL010000000
VERSION     DADMFL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.280"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..215
                     /locus_tag="JNZ27_004259"
     CDS             <1..215
                     /locus_tag="JNZ27_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666317.1"
                     /translation="IAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIA
                     VAAYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(256..1206)
                     /gene="corA"
                     /locus_tag="JNZ27_004260"
     CDS             complement(256..1206)
                     /gene="corA"
                     /locus_tag="JNZ27_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY8666318.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1576..>1906
                     /locus_tag="JNZ27_004261"
     CDS             1576..>1906
                     /locus_tag="JNZ27_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666319.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          493 a          472 c          470 g          471 t
ORIGIN      
        1 tcattgcttt cttctgttcc tggatctatt tatttgttct gggcttatgg aaaaaagcaa
       61 ttatagttct attacttggc attctatctc tatttgttgg tgcattaatt ggcgttaaca
      121 ttctgggaat tgccgtagca gcctatgttg ctgttaacac gaacaaatgg ttttatgaga
      181 aagaggtaaa agggttaaat acctggagcc tttgaataac aggtggctta gccaggctaa
      241 gccaccgcct tctttttaca accagttctt ccgcttaaag tacagatacg gtgccaggcc
      301 cgcgaggatc ataaagataa tcgcgccagg gtagccgaaa ctccacttca gttctggcat
      361 aaactcaaag ttcatcccgt agctggaagc aaccagcgtt ggcggcagga acacgacgga
      421 taccaccgag aaaattttga tgatgcggtt ctgctcgatg ttgataaaac ccattgccgc
      481 ctgcatcagg aagttcacct tctggaacag ggattcgtta tgcggcagca gggattcgat
      541 atcgcgcagg atttcacgcg cctgctccag ttgcccaccc ggtaaacgcg ctttacgcac
      601 caggaagttg agagcgcgct gggtatccat cagacacagg cgaactttcc agccgatatc
      661 ttccagttcc gccagcgtgg agagcgcctc gtcgtactca tcgccctgat gcccttccat
      721 aatcacccgg ctcaactgct ccaggtcgct atagatattt tcaatttcat ctgccaactg
      781 ttcgattttg gtttcgaaca gatccagcag caactcgtag gcgttaccgt ctaccatcga
      841 ctggctacgg gcacgcatac gatacagacg aaaagcgggc agttcacgct cacgcagagt
      901 aaacagacga ccatcacgga tggtaaatgc cacagtggag ttaccggcgt gatcttccgc
      961 atcttcaaag aagaagaagg agtgaatatg caggccgtcg tcgtcttcaa agaaacgtgc
     1021 cgatgcttcg atgtcttcca gttccgggcg ggttgccagg ctctggccaa gttcagattg
     1081 tacgcgcagt cgctcgtcgt cgtccggttc gacaagatca atccatactg catttacaag
     1141 gggttgtgac tcttcgactt ccagccgggt cagtcggtta ttttccagtt gaaatgcgct
     1201 cagcatgacc gggactccca atgcttaaaa tatcggacag ttcggtgggc acacagaaac
     1261 aaattgggtt tcagaccatt aaacagcctg actcagcgcg acgggaaaaa ttaaggtcgc
     1321 tgacaaccgc taaggctatc agcaaaaagg gatagcctta ggagttgatc ctggatgaca
     1381 ggatagtgag ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta gagtgatcgt
     1441 gcgcgcatgt tacgccagca aaattttgcc gtcaacacgc aacagaacac cacagaacaa
     1501 tagttgaact tcgcgctttt tggtactcgt caaggtcgct aacaggataa aaaaatccca
     1561 catccaggac ctataatggc gtatcggcta ctcattggta aaggaatgat caccttgaat
     1621 ctcaaacgga tttttctcac cctcacctta cttcccttgt ttgctgttgc cgctgatgat
     1681 tgcgcactct cagatccgac gctgaccgta caggcgtata ccgttaatcc tcaaacagag
     1741 cgggtgaaaa tgtactggca aaaagccaat ggcgaagcgt ggggaacgtt acatgctctg
     1801 ctggcggata ttaatagtca gggtcaggtg cagatggcga tgaacggcgg catctatgat
     1861 gaaagctatg cgccgctcgg tttgtacatc gaaaacggtc agcaga
//
LOCUS       DADMFL010000273         1876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.281, whole genome shotgun sequence.
ACCESSION   DADMFL010000273 DADMFL010000000
VERSION     DADMFL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.281"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..557)
                     /locus_tag="JNZ27_004262"
     CDS             complement(<1..557)
                     /locus_tag="JNZ27_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8666320.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JNZ27_004263"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JNZ27_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY8666321.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1544)
                     /locus_tag="JNZ27_004264"
                     /pseudo
     CDS             complement(1321..1544)
                     /locus_tag="JNZ27_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          538 a          316 c          394 g          628 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agtgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccagactt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tagtgaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 tgaaatagaa ttactgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac ccacaaatga
     1681 aatatacacc ggttggcgtt gatatggcaa aacatgtcat tcagattcac ttcatcaatg
     1741 agcacatgtc agcgccagtg atataagacg gtaatgggga tgcggacgat ttcctggact
     1801 ctcagaagat gaaacatgtt tactgaagaa caaaaaattc gcgctattga gctttatttc
     1861 aaatatggta agaagc
//
LOCUS       DADMFL010000274         1871 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.282, whole genome shotgun sequence.
ACCESSION   DADMFL010000274 DADMFL010000000
VERSION     DADMFL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.282"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            12..1043
                     /locus_tag="JNZ27_004265"
     CDS             12..1043
                     /locus_tag="JNZ27_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666322.1"
                     /translation="MTKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRT
                     TSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEEIIA
                     ENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDH
                     ANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNATEH
                     HQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVEGIS
                     DPNDSKWIPGHHISSNEVKNLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            1103..>1871
                     /locus_tag="JNZ27_004266"
     CDS             1103..>1871
                     /locus_tag="JNZ27_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8666323.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKT
                     GIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFKNF
                     LPHIEFLNLGGEHPGIDTSRDNSELFANDGTPTWLKEK"
BASE COUNT          555 a          456 c          449 g          411 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaatt
      901 tgcaacgcca cggtgccaat aaacaggcaa tcagccacgc atttgatgat gatgttgagt
      961 ttcaggagcg catggcagaa cacatccggt acatggctga aaccattgcc caccaccagg
     1021 ttgatattga tttggaggac taaaatctat ggcgcttttt caacgagcaa taaacacaca
     1081 ggcctatcta aaagcaggaa ttatggggtt tgctggcgac ggcaaaacct acacagcaag
     1141 cgaactggct attggtctgg taatgcttat gcggcaacgg ggaattgaag ctggtaaccg
     1201 cccggtaatg tttctggaca cagaaacagg ctctgactgg gttaaacccc gctttgatgc
     1261 ggaaaatatc gaattattta cggcaaaaac ccgctcgttt gttgatctgc ttgaagccat
     1321 taatgaagcg gaaagcagcg gttccgtaat gatcatcgac tccatcagtc acttctggac
     1381 cggattatgc gacgagtatg ccagacgccg aaaccgtaaa cgaggactgg aattttcaga
     1441 ctgggcgtgg ctcaaacagg agtggcgacg ctttaccgat cgatttgtta acagtcaggc
     1501 gcacattatt atgtgcgggc gtgcaggata tgagtatgac tttttcgaag gtgatgatgg
     1561 caaacgccag ttggcaaaaa ccggtataaa aatgaaagca gaaacagaaa ccggttatga
     1621 accctccatc ctgatccaga tggagaagca aatgaacata gaaaccggtc aggtgtggcg
     1681 caccgccaga atactaaaag atcgctccac ccgtattgat ggacaggtat tttcaaaccc
     1741 aacatttaaa aactttcttc ctcatatcga gtttcttaac ctgggaggtg aacaccccgg
     1801 catcgatacc tcacgggata acagtgaatt atttgccaac gacggtacac cgacatggct
     1861 aaaagaaaag c
//
LOCUS       DADMFL010000275         1869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.283, whole genome shotgun sequence.
ACCESSION   DADMFL010000275 DADMFL010000000
VERSION     DADMFL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.283"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            27..1869
                     /gene="cyoB"
                     /locus_tag="JNZ27_004267"
                     /pseudo
     CDS             27..1869
                     /gene="cyoB"
                     /locus_tag="JNZ27_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          358 a          484 c          504 g          523 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggtg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttataccta gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg
     1621 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     1681 ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     1741 aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag
     1801 gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     1861 ggctggcga
//
LOCUS       DADMFL010000276         1835 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.284, whole genome shotgun sequence.
ACCESSION   DADMFL010000276 DADMFL010000000
VERSION     DADMFL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.284"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..387
                     /locus_tag="JNZ27_004268"
     CDS             <1..387
                     /locus_tag="JNZ27_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HAY8666324.1"
                     /translation="NDDLKVFVMGEVGKQSTLKMDRSGMTLAEALGNAEGMNQDVADA
                     TGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPYDIVYVTTAPLARWNRV
                     ISLLVPTISGVHDLTETSRWIQTWPN"
     gene            375..821
                     /gene="etp"
                     /locus_tag="JNZ27_004269"
                     /gene_synonym="yccY"
     CDS             375..821
                     /gene="etp"
                     /locus_tag="JNZ27_004269"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY8666325.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            841..>1835
                     /locus_tag="JNZ27_004270"
     CDS             841..>1835
                     /locus_tag="JNZ27_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HAY8666326.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFMLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQSQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEIS"
BASE COUNT          498 a          433 c          489 g          415 t
ORIGIN      
        1 aacgatgatc tgaaagtctt cgtgatgggt gaagtgggta aacagagcac gctgaaaatg
       61 gaccgcagcg gtatgaccct ggcagaagcg ctgggtaacg ctgagggtat gaatcaggat
      121 gtcgctgatg cgacggggat ttttgttatt cgtgcaacgc aaaacaagca gaatggcaaa
      181 atcgccaata tttatcagct gaatgcgaag gacgcatcgg caatgatact cggaacggaa
      241 ttccagcttg aaccttacga cattgtttat gtcactaccg cgccgctggc tcgttggaac
      301 cgtgtgattt ctctgctggt accgacgatt tccggggtgc atgacctgac agagacttca
      361 cggtggattc aaacatggcc caactaaaat ttaactcaat cctagtggtt tgtaccggca
      421 acatttgccg ttcgcccatt ggcgagcgct tgctgcgtaa acgtctgccg ggcgtaaaag
      481 tcaaatcggc aggtgttcat ggtctggtaa aacaccctgc agatgcgaca gcggcagatg
      541 ttgccgctaa tcatggcgta tctcttgaag gacatgccgg acgtaagctc actgctgaga
      601 tggcacgaaa ttacgatctg attctggcaa tggagtcgga acatattgct caggttaccg
      661 caattgctcc cgaggtccgc gggaagacaa tgctgtttgg gcaatggctg gaacagaaag
      721 agatcccgga tccctatcgt aaaagtcagg acgcatttga acatgtctac ggtatgttgg
      781 agcgcgccag tcaggaatgg gcgaagcgcc tcagccggta atttgagttt ataaaatacg
      841 atgacaacta aaaatatgaa tacgccacta ggcagcactc aggaaaatga gatcgatctg
      901 cttcgtctgg tcggcgagtt atgggatcac cgtaagttta ttatcagcgt gaccgcgtta
      961 ttcatgctga tcgctgtcgc ttactcgctg ttaagcacac caatttatca ggcagatact
     1021 ctggtccagg ttgagcaaaa acagggcaac gccattctca gcggcctgag cgatatgctc
     1081 cctaactcat cgcccgagtc tgcaccggag atccaactgc tgcaatcgcg catgattctc
     1141 ggtaaaacca ttgctgaact gaatctgcgc gacatagttg agcagaagta ttttccgatt
     1201 gtgggtcgcg gctgggcgag attaaccaaa gaaaaaccag gtgagctggc gatcagctgg
     1261 atgcatattc cacaactgaa tggtcaggat cagcaactga cactcacggt tggggaaaac
     1321 ggccactata cactggaagg tgaagagttc accgtcaatg gtatggtcgg ccagcgtctg
     1381 gaaaaagatg gcgttgcgct gactatcgcg gacattaagg ccaaaccagg aacacagttt
     1441 gtcctgagcc agcgtaccga actggaagcg attaacgcat tgcaggaaac ctttaccgtt
     1501 agcgaacgca gtaaagaaag cgggatgctg gaacttacca tgactggtga tgatccccag
     1561 ttgattactc gtattctgaa cagcatcgct aacaactatt tgcaacagaa tatcgctcgc
     1621 caggcggcgc aggattcaca aagccttgaa ttcttacaga gccagttacc tgaagtgcgc
     1681 agcgagctgg accaggcgga agaaaaactc aacgtttatc gccagcagcg cgattcggtt
     1741 gaccttaacc tggaagccaa agccgttctt gagcagattg tgaacgttga taatcaactc
     1801 aatgagctga ccttccgcga ggcagagatc tccca
//
LOCUS       DADMFL010000277         1795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.285, whole genome shotgun sequence.
ACCESSION   DADMFL010000277 DADMFL010000000
VERSION     DADMFL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.285"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(81..1682)
                     /locus_tag="JNZ27_004271"
     CDS             complement(81..1682)
                     /locus_tag="JNZ27_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8666327.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQAPVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKAS
                     HAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDCL
                     DVVTINNKYLIKMAAVFKWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQL
                     LVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINEDD
                     SSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKNG
                     YGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSI
                     TYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          445 a          449 c          415 g          486 t
ORIGIN      
        1 ttgacaagta gagaaaacta tgagtcaaaa acatgaccac acaggtctgt agtaagtgtc
       61 atcctagact atggtctcct ctaggtctcc ttataaacat cagccatcac attgaccagc
      121 ctatcaacct cgacctcttt agcataacgg tcgtacgtaa tagaccctga actatgcccc
      181 atgatcgcct gtgcatacgc caactcgacc ccatgttgtt ttaactgggt cgcaactgta
      241 tgtctaaacg agtgaaacgc taagccgcta cggtctacga ggactttagg caacaacgtc
      301 cgattaaacc atttactcac ctgctcaccg taaccgttct tcatgagacg cagaccatcg
      361 aacaccatag cgtcatcacc gtcagcgcct ctacgggtct ctacaagcgc cataaagtca
      421 gccaatataa aaccataggc cccatcgacc agaggcacac agcggtcact atgagcgttc
      481 ttgatgctct taccgggtag actactatcg tcttcattga tatgaatata aacggttccc
      541 gcctcagtgg tcctgacgtc tttgacctgt agttgggcta tttcattgag tctcgcccca
      601 gtgatagcag ccaaagccgt aacgtaatag tggtagggct taccagaggt cttctgagag
      661 tatgccttag ctgcgaccag tagttgcccc acctgctccg tagagaaggc gttacgggcc
      721 cctgaggctt tacgctgagg gactttaagc tccagaccct cagtcatgtt ctttttgatc
      781 agatcattac gtacagccca cttgaagaca gcagccatct taataagata cttgttgttg
      841 atggtaacga catccagaca atctgtctta tcttcacggc tcaacaggtc agcaagtggg
      901 gcatccttaa agcgctgctt acggttctta ggtaactgct ggagcacatc acggacctta
      961 agcatgtcag ccctgttagc gtctgcattg agacccagat ggtcgaatat ttcgattaac
     1021 gcagcgtgtg acgctttgtt ttcccttaag gttgcaggct tccagttctg agagttctcc
     1081 gcttcgtatt gctcaaagag gctcgctatg gtaatgctag gcgtaacttc tggttttact
     1141 tctggagcac taacagatag ggacacaggg gcttggttat cttgcctatc aatatcatta
     1201 aaaagatcga cgtaatctat cagaggctga ctgtctcccg cttcgatacg cctcatacag
     1261 gcttttagca ccttcagggc ctcattgatg taacgatgct ggtcaatgct tagcggctca
     1321 ctagccagag catcggtaag gctctcacca agttctccgt attggtcccg gtagatatcc
     1381 ctcatgtcag gctcaaagag atctgaacgg cccatagaca gttcacactc agcgatgtcc
     1441 ttaaggtgct ccctcatctc ctcataggta gcgttgggtc tgtctgcgtg tattgccttg
     1501 agtgctgcct tgatgtgcct tgagtgagcc atagcggtgc gtctatcctt ggtccgtaat
     1561 gatactgagg tcattctatc attgtttgat tgtctgaccc gaagacggaa gtagtagacg
     1621 ccgttacgct ggtagagata gtagactgct ggaagtttgt gactactgga tagctgaatc
     1681 atatgtctgt atgacctgag agggttacac agtgggttac agtaagtaac tctgcaagga
     1741 ggcttaaagc catatccctt aggattcagt aagttggaat actttaagaa aggtc
//
LOCUS       DADMFL010000278         1790 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.286, whole genome shotgun sequence.
ACCESSION   DADMFL010000278 DADMFL010000000
VERSION     DADMFL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.286"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>1790
                     /locus_tag="JNZ27_004272"
     CDS             <1..>1790
                     /locus_tag="JNZ27_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY8666328.1"
                     /translation="NFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRT
                     ITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTTS
                     QYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALVG
                     VQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCLW
                     DMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRKA
                     WDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALK
                     DRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLWL
                     IKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDR
                     EITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLR
                     QRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHLT
                     AEVTADSGEYQVLERWDTPKVVKGVSFLLRL"
BASE COUNT          391 a          481 c          581 g          337 t
ORIGIN      
        1 aacttttccg gtgttacggt ggtgttccgc gccggcgagc aggagcagac accgccggag
       61 gggtttgaat cttccggctc agagactgtg ctgggtacgg aagtgaaata cgacacgccg
      121 atcacccgga ccatcacgtc ggcaaacatt gatcgtctgc gcctgacctt cggtgtgcag
      181 gcactggtgg aaaccacctc aaagggggac cggaatccgt cggaagtccg cctgctggtt
      241 cagatacagc gtaacggtgg ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc
      301 acttcgcagt atctggcctc ggtggtggtg ggtaacctgc cgccgcgccc gtttaatatc
      361 cggatgcgca ggatgacacc ggacagcacc acagaccagc tgcagaacaa aacgctctgg
      421 tcgtcgtaca ccgaaatcat cgatgtgaaa cagtgctacc cgaacacggc gctggtcggc
      481 gtgcaggtgg attcagagca gttcggtaac cagcaggtga gtcgcaatta tcatcttcgc
      541 gggcgcattc tgcaggtgcc gtcgaactat aacccgcaga cgcggcaata cagcggtatc
      601 tgggacggaa cgcttaagcc agcatacagc aacaacatgg cctggtgtct gtgggacatg
      661 ctcactcatc cgcgctacgg catggggaaa cgtcttggtg cggcagatgt ggacaaatgg
      721 gcgctgtatg tcatcggcca gtactgcgac cagtcagtgc cggacggatt tggcggcacg
      781 gagccgcgca tcacctgtaa cgcttacctg accacacagc gtaaggcgtg ggatgttctc
      841 agtgatttct gctcggcgat gcgctgtatg ccggtatgga acgggcagac gctgacgttc
      901 gtgcaggacc gaccgtcgga taaagtgtgg acctataacc gcagtaatgt ggtgatgccg
      961 gatgatggcg cgccgttccg ctacagcttc agcgccctga aggaccgcca taatgtcgtt
     1021 gaggtgaact ggattgatcc ggacaacggt catgagacgg cgacagagct tgtggaggac
     1081 acgcaggcca ttgtccgtta cggtcgtaac gtcacgaaga tggatgcctt tggctgtacc
     1141 agccggggac aggcgcaccg cgccgggctg tggctgatta aaacggaact gctggaaacg
     1201 cagaccgtgg acttcagcgt cggcgcagaa gggcttcgcc atgtaccggg tgatgtcatt
     1261 gaaatctgcg atgatgatta tgccggtatc agcaccggtg gtcgcgtact ggcggtgaac
     1321 agccagaccc ggacgctgac gctcgaccgt gaaatcacgc tgccgtcctc cggtaccacg
     1381 ctgataagcc tggttgacgg aaatggcaat ccggtcagcg tggaggttca gtccgtcacc
     1441 gacggcgtga aggtgaaagt gagccgggtt cctgacggcg ttgcagaata cagcgtgtgg
     1501 gggctgaagc tgccgacgct gcgccagcgc ctgttccgct gcgtgagtat ccgtgagaac
     1561 gacgacggca cgtatgccat caccgccgtg cagcatgtgc cggaaaaaga ggccatcgtg
     1621 gataacgggg cacactttga cggtgaccgg cgcggcacgg tgaatggtgt cacgccgcca
     1681 gcggtgcagc atctgaccgc cgaagtcacc gcagacagcg gggaatatca ggtgctggag
     1741 cgatgggaca cgccgaaggt ggtgaagggt gtgagcttcc tgcttcgcct
//
LOCUS       DADMFL010000279         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.287, whole genome shotgun sequence.
ACCESSION   DADMFL010000279 DADMFL010000000
VERSION     DADMFL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.287"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..284
                     /locus_tag="JNZ27_004273"
     CDS             <1..284
                     /locus_tag="JNZ27_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666329.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JNZ27_004274"
                     /pseudo
     CDS             286..890
                     /locus_tag="JNZ27_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JNZ27_004275"
     CDS             877..>1773
                     /locus_tag="JNZ27_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666330.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREVDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          426 a          402 c          495 g          450 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 tcagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaagtc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADMFL010000280         1741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.288, whole genome shotgun sequence.
ACCESSION   DADMFL010000280 DADMFL010000000
VERSION     DADMFL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.288"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            88..153
                     /locus_tag="JNZ27_004276"
                     /pseudo
     CDS             88..153
                     /locus_tag="JNZ27_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JNZ27_004277"
     CDS             381..629
                     /locus_tag="JNZ27_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8666331.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="JNZ27_004278"
     CDS             complement(745..1014)
                     /locus_tag="JNZ27_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8666332.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            1071..1739
                     /locus_tag="JNZ27_004279"
     CDS             1071..1739
                     /locus_tag="JNZ27_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8666333.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          518 a          378 c          371 g          474 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttcttcc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc ctaaagcttt
     1141 atgactggtg ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac
     1201 accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa
     1261 ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg
     1321 aagctagcct gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta
     1381 gccatgatgt ttttgaacct tatcctgcgg cgttacatgg tcaatttgat tccatttcca
     1441 tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca
     1501 atgcggcgca ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg
     1561 gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct
     1621 tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga
     1681 atgttaaaac caaggttcaa ggtactgtag taatgttttc cgcttcaggg aaaaaatagc
     1741 a
//
LOCUS       DADMFL010000281         1731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.289, whole genome shotgun sequence.
ACCESSION   DADMFL010000281 DADMFL010000000
VERSION     DADMFL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.289"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(219..773)
                     /locus_tag="JNZ27_004280"
     CDS             complement(219..773)
                     /locus_tag="JNZ27_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY8666334.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="JNZ27_004281"
     CDS             complement(770..1060)
                     /locus_tag="JNZ27_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8666335.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="JNZ27_004282"
     CDS             complement(1060..1659)
                     /locus_tag="JNZ27_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY8666336.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          415 a          477 c          394 g          445 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcgtgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata c
//
LOCUS       DADMFL010000282         1718 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.290, whole genome shotgun sequence.
ACCESSION   DADMFL010000282 DADMFL010000000
VERSION     DADMFL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.290"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            390..626
                     /locus_tag="JNZ27_004283"
     CDS             390..626
                     /locus_tag="JNZ27_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666337.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARA
                     QIESQRYADRQRALREDERQQSDFAWSRPRAPRR"
     gene            840..1244
                     /locus_tag="JNZ27_004284"
     CDS             840..1244
                     /locus_tag="JNZ27_004284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666338.1"
                     /translation="MKILAAMEIADCDFEHMDMDVLVGALLKLKLWDSHHHNLNMQGK
                     RSFLDDLRADGIEAIKKYEAEIESENESFAEERLILRIYQNELGLWLGRLFCGEAEIG
                     QCGVYGSADEVRTAARQSGILCDSIEIFQKDA"
BASE COUNT          393 a          401 c          486 g          438 t
ORIGIN      
        1 aaaaaagttg tagtgcccgc cgcagtctca cgtccggagc gtagcgaccg agtgagcgag
       61 gaggcatcat ttcactattc gggcatggta ttgcggacgc ttctgagcca catatcacac
      121 ctgccacatg acgcactttc ctgataatcg cacctatgcc aacatattaa gccagtatac
      181 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct aaatccactg
      241 acgcgcctgc ggcttgtcca acgcctcacg gcaccggatt aaaatccgct cccgttcacc
      301 gtttctgact gcctttcacc gtcgcttacg cttcatcgtt cctactgtgt ctggcagact
      361 gcaggggcta ttctcatacg gagataaaca tgacgagact gacaaacctg acccccgctg
      421 agaaaaagtt tatcgacgac gctatagccg ctgccgaacg tgcggccggg aagaagctca
      481 atcagcctaa ccggcacatc gtcctgaaca gagcgcgtgc gcagattgag tcgcagcgtt
      541 acgccgaccg ccagcgtgca ttacgtgagg atgagcggca gcagtccgat tttgcctggt
      601 cacgtccgcg cgcgccgcgc cgctgattgc ggattttggc ggtttagtgg tgctgatgct
      661 gcgaaatcac ctgaggttca cccgcgtgca tagctatgca gtcgcgcaga aatcatcagg
      721 ggcgtttttc cgggatgtgg gtgaggaaat gtgccggaaa cggctctgat gcgtcagaat
      781 tgcgctgagg gcctgtcagg gggtgctgat aacggatgtt atggtaaatc ggagggtgta
      841 tgaagatttt ggctgcaatg gaaatagctg attgtgattt tgaacatatg gatatggatg
      901 tcttagtagg ggctttactg aaattaaaat tgtgggatag ccaccatcac aacttgaaca
      961 tgcaaggtaa gaggagcttt ctcgatgatt taagagcgga tggtattgag gctattaaga
     1021 aatacgaggc tgaaattgaa tctgagaatg agtcctttgc ggaggagcga ttaatactga
     1081 ggatctatca gaacgagctt ggcttatggt tagggaggct cttttgtggt gaggctgaga
     1141 tcggtcagtg tggtgtttat ggatcggctg atgaggttcg aacagctgcg cgtcaaagcg
     1201 gtattttgtg cgatagcatc gaaatttttc aaaaggatgc ctaatgcccc cgtggtgggt
     1261 acaccagaaa acggggagcg gagcgaccag cgtcaggtcg ttctatgttg ttctgattgt
     1321 gtgggtgttg tgccagactg ggcaatcgca tttcaccgca actcctgcct tgaatctcac
     1381 tttatcaggt gcctgttatc gtacagcgag acggggtaga gcttaactct ttcgcggtta
     1441 agttacgttt ccaccgtttg cctgtttatc ctgccggagt ccggggcgta gacctgagtt
     1501 tcaagggtgc agatatgatt ttaatgtcgt tgaatggtat taagctggta gtgacaaggc
     1561 tagccagccc tgctaggata ggattgctac gtctgatcct ggcaggtatc tgcgaagaat
     1621 tagagtaccg aagccctgtg attctgtcac ggggcttttg tacatctgta gcctgatctt
     1681 tcaacaagat ccccgtcaca cctcacttca tgtatgtg
//
LOCUS       DADMFL010000283         1718 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.291, whole genome shotgun sequence.
ACCESSION   DADMFL010000283 DADMFL010000000
VERSION     DADMFL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.291"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..219)
                     /locus_tag="JNZ27_004285"
     CDS             complement(<1..219)
                     /locus_tag="JNZ27_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8666339.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="JNZ27_004286"
     CDS             525..1307
                     /locus_tag="JNZ27_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY8666340.1"
                     /translation="MEAKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1407)
                     /locus_tag="JNZ27_004287"
     CDS             complement(1309..1407)
                     /locus_tag="JNZ27_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY8666341.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          480 a          409 c          344 g          485 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gcatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa gcgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcact
//
LOCUS       DADMFL010000284         1679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.292, whole genome shotgun sequence.
ACCESSION   DADMFL010000284 DADMFL010000000
VERSION     DADMFL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.292"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(72..539)
                     /locus_tag="JNZ27_004288"
     CDS             complement(72..539)
                     /locus_tag="JNZ27_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8666342.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="JNZ27_004289"
     CDS             complement(605..844)
                     /locus_tag="JNZ27_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY8666343.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="JNZ27_004290"
     CDS             complement(979..1584)
                     /locus_tag="JNZ27_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8666344.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          542 a          370 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DADMFL010000285         1545 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.293, whole genome shotgun sequence.
ACCESSION   DADMFL010000285 DADMFL010000000
VERSION     DADMFL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.293"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(276..350)
                     /locus_tag="JNZ27_004291"
     tRNA            complement(276..350)
                     /locus_tag="JNZ27_004291"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Gly,seq:tcc)
     gene            complement(354..429)
                     /locus_tag="JNZ27_004292"
     tRNA            complement(354..429)
                     /locus_tag="JNZ27_004292"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Thr,seq:tgt)
     gene            complement(435..508)
                     /locus_tag="JNZ27_004293"
     tRNA            complement(435..508)
                     /locus_tag="JNZ27_004293"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(472..474),aa:Arg,seq:tct)
     gene            complement(510..585)
                     /locus_tag="JNZ27_004294"
     tRNA            complement(510..585)
                     /locus_tag="JNZ27_004294"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Ile,seq:cat)
     gene            complement(762..1451)
                     /locus_tag="JNZ27_004295"
     CDS             complement(762..1451)
                     /locus_tag="JNZ27_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY8666345.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRLEQKA
                     AFSKQSIAKVD"
BASE COUNT          401 a          413 c          330 g          401 t
ORIGIN      
        1 cgccagccaa ggcagttaag agtgtgtccc cggggtttgc ttagaatatt ttgtaagttg
       61 tccgaagaga tatttacaac accagaatga tgaatcatca gtcccctgcc agaggttcac
      121 tacgtaacct gaccgacaat gcaccattct ggtgctgaaa aaagagcact ggaactgcaa
      181 caaaacaaca tcacagaaca ggagatggta aggagtcaga cattcccaca caatattgtg
      241 tcagtgcaaa taacaacctt cgtctcaaat ctcgctggag cgggcagcgg gaatcgaacc
      301 cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc atatggtgcc
      361 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
      421 taagtcggcg caggcccacc accgaggact tgaacctcgg accgtcaact tagaaggttg
      481 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      541 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      601 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      661 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      721 aaaatcggca aaatatcaga tttgcatgaa atatatgcct tttaatctac ttttgcaata
      781 ctttgctttg aaaatgccgc tttttgttct aaacgtgttc tcatcacaaa caataaagcc
      841 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      901 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      961 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
     1021 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
     1081 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
     1141 caggctttat ggtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
     1201 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
     1261 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
     1321 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1381 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1441 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1501 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcga
//
LOCUS       DADMFL010000286         1489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.294, whole genome shotgun sequence.
ACCESSION   DADMFL010000286 DADMFL010000000
VERSION     DADMFL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.294"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            82..1275
                     /locus_tag="JNZ27_004296"
     CDS             82..1275
                     /locus_tag="JNZ27_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY8666346.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1301..1489)
                     /locus_tag="JNZ27_004297"
                     /pseudo
     CDS             complement(1301..1489)
                     /locus_tag="JNZ27_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          438 a          326 c          350 g          375 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttaccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcggcacacc accaaagaag cggaacgcat tatgatggca ggtctccag
//
LOCUS       DADMFL010000287         1469 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.295, whole genome shotgun sequence.
ACCESSION   DADMFL010000287 DADMFL010000000
VERSION     DADMFL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.295"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(88..726)
                     /locus_tag="JNZ27_004298"
     CDS             complement(88..726)
                     /locus_tag="JNZ27_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666347.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(885..1355)
                     /locus_tag="JNZ27_004299"
                     /pseudo
     CDS             complement(885..1355)
                     /locus_tag="JNZ27_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          423 a          361 c          258 g          427 t
ORIGIN      
        1 atatccaagc ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg
       61 ctcaaatcta tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact
      121 gttagcatag tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc
      181 atctacaaat gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt
      241 ttgaacatcc aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc
      301 ctgttcaata gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc
      361 aatacgctca cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc
      421 aatttgctct aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac
      481 gggagcctct cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga
      541 acagttacca tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga
      601 atctttatcc atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt
      661 tacatttccc tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt
      721 aatcatattg tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc
      781 tctgatatga caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc atctattcgt
      841 gtctaaaaag tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg
      901 gctaacagtt cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg
      961 ccagttttag tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa
     1021 gcgccactgc tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga
     1081 tgttatccgc cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc
     1141 agtgtcctgg cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc
     1201 ttaacgccgg gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag
     1261 cgacaacgga aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa
     1321 tgacagcgcg gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt
     1381 accagaaact tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt
     1441 tacgaggttc ttttcgccag tcctgtaat
//
LOCUS       DADMFL010000288         1448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.296, whole genome shotgun sequence.
ACCESSION   DADMFL010000288 DADMFL010000000
VERSION     DADMFL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.296"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(123..605)
                     /locus_tag="JNZ27_004300"
     CDS             complement(123..605)
                     /locus_tag="JNZ27_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8666348.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(596..898)
                     /locus_tag="JNZ27_004301"
     CDS             complement(596..898)
                     /locus_tag="JNZ27_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8666349.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1170..1442
                     /locus_tag="JNZ27_004302"
     CDS             1170..1442
                     /locus_tag="JNZ27_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666350.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          421 a          363 c          304 g          360 t
ORIGIN      
        1 tttttctgct cattattggc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcagac tcagactcag actcagactc agactcagac tcagactcag
      121 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
      181 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      241 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      301 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      361 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      421 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      481 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      541 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      601 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      661 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      721 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      781 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      841 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      901 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      961 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
     1021 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
     1081 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1141 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1201 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1261 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1321 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1381 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1441 gattttca
//
LOCUS       DADMFL010000289         1401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.297, whole genome shotgun sequence.
ACCESSION   DADMFL010000289 DADMFL010000000
VERSION     DADMFL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.297"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>1401
                     /locus_tag="JNZ27_004303"
     rRNA            <1..>1401
                     /locus_tag="JNZ27_004303"
                     /product="23S ribosomal RNA"
BASE COUNT          386 a          299 c          443 g          273 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac g
//
LOCUS       DADMFL010000290         1392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.298, whole genome shotgun sequence.
ACCESSION   DADMFL010000290 DADMFL010000000
VERSION     DADMFL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.298"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..465
                     /locus_tag="JNZ27_004304"
     CDS             <1..465
                     /locus_tag="JNZ27_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666351.1"
                     /translation="AFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAELGDD
                     SGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSN
                     TVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            525..1013
                     /locus_tag="JNZ27_004305"
     CDS             525..1013
                     /locus_tag="JNZ27_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8666352.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEG
                     SS"
     gene            1037..>1392
                     /locus_tag="JNZ27_004306"
     CDS             1037..>1392
                     /locus_tag="JNZ27_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666353.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          391 a          302 c          372 g          327 t
ORIGIN      
        1 gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag cgccaacaac
       61 tccacgaaaa atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt tgttgcagag
      121 cttggtgatg attcaggctg gcattttttc agccagagaa acccggataa cagcatagtg
      181 ttcactgtta acggacaggt aattccgtta aattacggaa acttcgatgc ccgctataaa
      241 tatcgaacag agggggtaca ggatgtacgg tatggccatg aaatgtatta cagccccggc
      301 agtaacaccg tttcgtggag attttgcgca ccttcgggac acgggctgtc agggatggcg
      361 atatcggata ccggccgtaa ctcagcggat aacgttgacg gtgtgtatta ccgaccactg
      421 caaaaactga ttaatggcac ctggtataac gtagcgagta tttaacaatg ttgcatttaa
      481 aaaatattac tgcgggtaat ccgaaaacag cagaacaata tcagatgaca aaacgatatt
      541 cggtcacctg gcttttttca gaagacggaa aaaactggta tgaagagctg aagaatttcg
      601 ccagcgacac aataaaaata gcttacaccg gagatggtcg cgtggtgtgg gtcggtaagg
      661 atgtgacagg catcgagcca cgcaatgcca gcgttatcga agttccggat attaccgcta
      721 accgacggat taccgcgccg ggttactggt tttaccgcaa tgatgaattt gtctttgact
      781 acagactcaa agcggaagat gagcgtgatg cccttctggc tcaggtcagt gcccggacag
      841 gggaatggga agaagacctg ctgctggggt taatcagcga cgaagataaa gaaaagctga
      901 aagcctgtcg tatttacgcg aaatcgctgc aggcgatgga tttcagcacc atcactgata
      961 aatcctcata caacgccatt gaatggcccg cctctccgga aggttcttcc tgatttaatt
     1021 tatcgcgaga aaaacaatgt ctgtagtgat atcaggtgcg ctgactgatg gcgcaggtat
     1081 ccccatgtcc ggataccata ttattctgaa atcccgggta aacaccccgg aagtggtgat
     1141 gaacactgtt gctgatgtga tgacaggaaa cgatggtgaa tactgtttcc atgcgcggac
     1201 tggaaaatat ggtgtgtatc tgaaacagga ctggcgcaac gagtacaacg ttggcgacat
     1261 tgctgtatat gaggactcaa agcccggcac gctgaatgac tttctgattg ctcctgatga
     1321 gggcgacctg aaaccggatg tcgtcaaacg ctttgaggaa atggtggcgc aggcgcagca
     1381 gagcgccggg gc
//
LOCUS       DADMFL010000291         1355 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.299, whole genome shotgun sequence.
ACCESSION   DADMFL010000291 DADMFL010000000
VERSION     DADMFL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.299"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            193..462
                     /locus_tag="JNZ27_004307"
     CDS             193..462
                     /locus_tag="JNZ27_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666354.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..571
                     /locus_tag="JNZ27_004308"
                     /pseudo
     CDS             459..571
                     /locus_tag="JNZ27_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="JNZ27_004309"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="JNZ27_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY8666355.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          262 c          332 g          372 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat tacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat gggga
//
LOCUS       DADMFL010000292         1322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.300, whole genome shotgun sequence.
ACCESSION   DADMFL010000292 DADMFL010000000
VERSION     DADMFL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.300"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>1322
                     /locus_tag="JNZ27_004310"
     rRNA            <1..>1322
                     /locus_tag="JNZ27_004310"
                     /product="23S ribosomal RNA"
BASE COUNT          332 a          297 c          411 g          282 t
ORIGIN      
        1 cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag
       61 aataccaagg cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt
      121 cgggagaagg cacgctgata cgtaggtgaa gcgacttgct cgtggagctg aaatcagtcg
      181 aagataccag ctggctgcaa ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg
      241 gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta attgatgggg ttagcgcaag
      301 cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa ctataacggt cctaaggtag
      361 cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg cgtaatgatg gccaggctgt
      421 ctccacccga gactcagtga aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag
      481 acggaaagac cccgtgaacc tttactatag cttgacactg aacattgagc cttgatgtgt
      541 aggataggtg ggaggctttg aagtgtggac gccagtctgc atggagccga ccttgaaata
      601 ccacccttta atgtttgatg ttctaacgtg gacccgtgat ccgggttgcg gacagtgtct
      661 ggtgggtagt ttgactgggg cggtctcctc ctaaagagta acggaggagc acgaaggttg
      721 gctaatcctg gtcggacatc aggaggttag tgcaatggca taagccagct tgactgcgag
      781 cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat ccggtggttc tgaatggaag
      841 gaccatcgct caacggataa aaggtactcc ggggataaca ggctgatacc gcccaagagt
      901 tcatatcgac ggcggtgttt ggcacctcga tgtcggctca tcacatcctg gggctgaagt
      961 aggtcccaag ggtatggctg ttcgccattt aaagtggtac gcgagctggg tttagaacgt
     1021 cgtgagacag ttcggtccct atctgccgtg ggcgctggag aactgagggg ggctgctcct
     1081 agtacgagag gaccggagtg gacgcatcac tggtgttcgg gttgtcatgc caatggcact
     1141 gcccggtagc taaatgcgga agagataagt gctgaaagca tctaagcacg aaacttgccc
     1201 cgagatgagt tctccctgac cctttaaggg tcctgaagga acgttgaaga cgacgacgtt
     1261 gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga
     1321 gg
//
LOCUS       DADMFL010000293         1291 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.301, whole genome shotgun sequence.
ACCESSION   DADMFL010000293 DADMFL010000000
VERSION     DADMFL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.301"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..815)
                     /locus_tag="JNZ27_004311"
     CDS             complement(<1..815)
                     /locus_tag="JNZ27_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8666356.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JNZ27_004312"
                     /pseudo
     CDS             907..993
                     /locus_tag="JNZ27_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JNZ27_004313"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JNZ27_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          362 a          218 c          335 g          376 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc cagactattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt c
//
LOCUS       DADMFL010000294         1277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.302, whole genome shotgun sequence.
ACCESSION   DADMFL010000294 DADMFL010000000
VERSION     DADMFL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.302"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNZ27_004314"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNZ27_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY8666357.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          361 a          229 c          284 g          403 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtgga
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADMFL010000295         1258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.303, whole genome shotgun sequence.
ACCESSION   DADMFL010000295 DADMFL010000000
VERSION     DADMFL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.303"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..165
                     /locus_tag="JNZ27_004315"
                     /pseudo
     CDS             1..165
                     /locus_tag="JNZ27_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            182..700
                     /locus_tag="JNZ27_004316"
                     /pseudo
     CDS             182..700
                     /locus_tag="JNZ27_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1067..1258
                     /locus_tag="JNZ27_004317"
                     /pseudo
     CDS             1067..1258
                     /locus_tag="JNZ27_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          386 a          284 c          265 g          323 t
ORIGIN      
        1 ggtcgtgaca gactggcacg tcttcgtaag gagctgaggc tgcactgtaa gcagaaacgc
       61 aagttcaggg cgaccacaaa ctctgaccac aatctgccgg ttacgccgaa tctgctgaat
      121 cagaacttta cgcccacagc acctaatcag gtctgggtcg cagattcagt tgtccaggcc
      181 ttccgaaatc aaccgactga aggcgctggt cgcgaaactg cagcgtatgc agttcggtaa
      241 aagctcagaa aaacttcgcg caaaaaccga acggcggata caggaagcac aggagcgaat
      301 cagcgcactt caggaggaaa tggcggaaac actgggtgag caatatgacc cggtactgcc
      361 atcttccctg cgccagtctt cagcccgtaa accgttacca gcctcacttc cccgtgcccc
      421 ccgggtcatc cggccggaag aggaatgctg tccggcctgt ggtggtgaac tcagtccact
      481 gggatgtgat gtgtcagagc agctggagct tatcagcagc gcctttaagg ttatcgaaaa
      541 acaacgccct aaactggcct gtcgccggtg cgaccatatc gtgcaggcac cggtgccttc
      601 aaaacccatt gcacgcagtt atgccggagc ggggcttctg gcccatgttg ttaccggaaa
      661 gtatgcagac catctgccat tataccgcca aagtgatcta ctattccaca cagctattta
      721 gctctctttt tgttgagctc ccatgattct ttcatttatt gttactaatt attttatgtt
      781 atcatgatgt tagaacacgc tctgctctgt gtctctattt tatctatcat attctgagga
      841 ttaactacat aacaaggcgc aagatatata tccaaaaaca aataataccg ttgcaataat
      901 tcctttcagt aaaggaggtg tgattaattt caggggattt cagagtaaaa attgtacaac
      961 atattaaaat atgttcattt cgttataata cacacaagaa aatattaagt tagataatat
     1021 attctaaata accatagata aaaacgcaca taatggggaa tattttatga aaatacctga
     1081 agcagtaaat catattaatg ttcagaataa tattgatctt gttgatggaa aaacgaatcc
     1141 gaataaagca acaaaagcat tacaaaaaat atattacgcg taacaaactc atcttcttct
     1201 ggcataagtg aaaaacattt agatcactgt gctaatactg tgaaaaactt tttaagga
//
LOCUS       DADMFL010000296         1250 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.304, whole genome shotgun sequence.
ACCESSION   DADMFL010000296 DADMFL010000000
VERSION     DADMFL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.304"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNZ27_004318"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNZ27_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY8666358.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          371 a          206 c          298 g          375 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acgtcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca
//
LOCUS       DADMFL010000297         1248 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.305, whole genome shotgun sequence.
ACCESSION   DADMFL010000297 DADMFL010000000
VERSION     DADMFL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.305"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..171)
                     /locus_tag="JNZ27_004319"
     CDS             complement(<1..171)
                     /locus_tag="JNZ27_004319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666359.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1248
                     /locus_tag="JNZ27_004320"
     CDS             308..>1248
                     /locus_tag="JNZ27_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666360.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFR"
BASE COUNT          327 a          312 c          363 g          246 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcg
//
LOCUS       DADMFL010000298         1227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.306, whole genome shotgun sequence.
ACCESSION   DADMFL010000298 DADMFL010000000
VERSION     DADMFL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.306"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..884
                     /locus_tag="JNZ27_004321"
     CDS             <1..884
                     /locus_tag="JNZ27_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8666361.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYAEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1227
                     /locus_tag="JNZ27_004322"
     CDS             884..>1227
                     /locus_tag="JNZ27_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8666362.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          280 c          317 g          305 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgccga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgtg
//
LOCUS       DADMFL010000299         1204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.307, whole genome shotgun sequence.
ACCESSION   DADMFL010000299 DADMFL010000000
VERSION     DADMFL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.307"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            14..925
                     /locus_tag="JNZ27_004323"
     CDS             14..925
                     /locus_tag="JNZ27_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8666363.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JNZ27_004324"
     CDS             941..1168
                     /locus_tag="JNZ27_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666364.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          338 a          255 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atgg
//
LOCUS       DADMFL010000300         1196 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.308, whole genome shotgun sequence.
ACCESSION   DADMFL010000300 DADMFL010000000
VERSION     DADMFL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.308"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(569..748)
                     /locus_tag="JNZ27_004325"
                     /pseudo
     CDS             complement(569..748)
                     /locus_tag="JNZ27_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            797..1196
                     /locus_tag="JNZ27_004326"
                     /pseudo
     CDS             797..1196
                     /locus_tag="JNZ27_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          328 a          224 c          311 g          333 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct ccagacaacc
      781 aatatcactt atttaagtga tagtcttaat actagttttt agactagtca ttggagagca
      841 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
      901 tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc ggcaacacgg
      961 tgtggcagcc agccagttat ttctctggcg caagcaatac caggagggaa gtcttactgc
     1021 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgctgcca tgaagcagat
     1081 taaagaactc cagcgcctgc tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc
     1141 cgttgaatat gggcgtgcaa aaaagtggat agcgcacgcg cccttattgc ccgggg
//
LOCUS       DADMFL010000301         1168 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.309, whole genome shotgun sequence.
ACCESSION   DADMFL010000301 DADMFL010000000
VERSION     DADMFL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.309"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(67..192)
                     /locus_tag="JNZ27_004327"
                     /pseudo
     CDS             complement(67..192)
                     /locus_tag="JNZ27_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(264..1133)
                     /locus_tag="JNZ27_004328"
     CDS             complement(264..1133)
                     /locus_tag="JNZ27_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY8666365.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          305 a          336 c          247 g          280 t
ORIGIN      
        1 acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat tcagtgtgcc
       61 acttcaccct agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga
      121 aataaaaaag agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat
      181 attcagtgcc ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga
      241 gcgacttcgc cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt
      301 gcccctttcg ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc
      361 atttctctct catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg
      421 aacgcatctc ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga
      481 acttgtcctc gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg
      541 atctcatact cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata
      601 agtcgccatt cttcttccga gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa
      661 cacaagcgga gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg
      721 acaaaacctc cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc
      781 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
      841 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      901 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      961 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
     1021 tcaccgaccc gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg
     1081 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
     1141 acgaccgcat cccctaaaac ccatactt
//
LOCUS       DADMFL010000302         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.310, whole genome shotgun sequence.
ACCESSION   DADMFL010000302 DADMFL010000000
VERSION     DADMFL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.310"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            2..385
                     /locus_tag="JNZ27_004329"
     CDS             2..385
                     /locus_tag="JNZ27_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8666366.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="JNZ27_004330"
     CDS             complement(386..1030)
                     /locus_tag="JNZ27_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8666367.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          262 c          278 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DADMFL010000303         1122 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.311, whole genome shotgun sequence.
ACCESSION   DADMFL010000303 DADMFL010000000
VERSION     DADMFL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.311"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>1122
                     /gene="pdeF"
                     /locus_tag="JNZ27_004331"
     CDS             <1..>1122
                     /gene="pdeF"
                     /locus_tag="JNZ27_004331"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8666368.1"
                     /translation="TYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLP
                     LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFT
                     PRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGT
                     LINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLAL
                     GALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSY
                     QNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLP
                     NVRALNRALRDALWSALCYLRIPSMEMLVKNYGIMLRIQ"
BASE COUNT          246 a          254 c          250 g          372 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
       61 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      121 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      181 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      241 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      301 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      361 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      421 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      481 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      541 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      601 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      661 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      721 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      781 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      841 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
      901 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
      961 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1021 atcgcgcgtt gcgtgatgcc ctctggtctg cgctttgtta tttacgcatc cctagcatgg
     1081 aaatgctggt taagaactat ggcatcatgc tgcggattca at
//
LOCUS       DADMFL010000304         1110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.312, whole genome shotgun sequence.
ACCESSION   DADMFL010000304 DADMFL010000000
VERSION     DADMFL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.312"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..1019
                     /locus_tag="JNZ27_004332"
                     /pseudo
     CDS             1..1019
                     /locus_tag="JNZ27_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1110
                     /locus_tag="JNZ27_004333"
     CDS             992..>1110
                     /locus_tag="JNZ27_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY8666369.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          316 a          270 c          255 g          269 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct agcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DADMFL010000305         1110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.313, whole genome shotgun sequence.
ACCESSION   DADMFL010000305 DADMFL010000000
VERSION     DADMFL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.313"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..176)
                     /locus_tag="JNZ27_004334"
     CDS             complement(<1..176)
                     /locus_tag="JNZ27_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8666370.1"
                     /translation="MGSTRVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSK
                     APFTVIPPVSRLEPS"
     gene            complement(344..838)
                     /locus_tag="JNZ27_004335"
     CDS             complement(344..838)
                     /locus_tag="JNZ27_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8666371.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          364 a          216 c          197 g          333 t
ORIGIN      
        1 gaaggttcta atcgtgatac tggcggaata acggtaaacg gcgctttgct tttatcctct
       61 ttattaaatg gatctaccca agcctggatg agataaggta ttttttcttc tttgctaaat
      121 acagaaatag atttatcagc attattactt aagtaaacca cccgcgtact accaatgaca
      181 acgcctgcat aagttaatgc tggttggcct aaaaaaacgc tgagtataat cgcggtaaat
      241 aacttacatt aaactgttgt tttaaagagc ctgattaaac aactttttct ggacataacg
      301 actccctggc caaagccaat tgaataaaaa ggcccttgat aaattatttc ttatattcga
      361 cagtaaattg tgaatcagcg ttcgccgtac cagccgttac ggtcgcattg gttgccacat
      421 agcgtgcaat aaacttcatt tcagccttgc cttcagcatc gatagcggta taatcagagt
      481 cgttataaag tttaacctgc gaattatcgg actggttaaa aatacgaata ccaacgccgg
      541 ttgccgcaat cgcttgtcct gtaccatcaa aacgaacggc tgcttgagtg tacgttgccg
      601 ggcaattttc aagtttaata ctgaacttct gtggcgacgc cagactacct acacctgaaa
      661 atgcattttt actgacttta cccagtggga caatttgatc ggctgtatcg gaggtaactt
      721 cacaagtagt gtcgacaatt tcaccgacga agttaacaac accatcttct gccagtgcat
      781 gcccggccag cattagagat gccagagtga atgcaccgcc tataatatta cgcttcataa
      841 aaactccatt gatataatat atcctttacc atgaatcatc acttatatta ataagtgtat
      901 ttttgattca aaggaatagt acaccaaaga gacggcttta tctaaaaata atattaatta
      961 cgcaaaaata aaatatagat ttattgttta aatattattt aaacagtaaa aaatcaagtt
     1021 tgttatcaga cattaatgat aattcagaat atatttatat agaaagatag atgaatttat
     1081 tgatgtcagt tataaaaaag ggccaccggt
//
LOCUS       DADMFL010000306         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.314, whole genome shotgun sequence.
ACCESSION   DADMFL010000306 DADMFL010000000
VERSION     DADMFL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.314"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..387)
                     /locus_tag="JNZ27_004336"
     CDS             complement(<1..387)
                     /locus_tag="JNZ27_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8666372.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JNZ27_004337"
     CDS             816..1097
                     /locus_tag="JNZ27_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666373.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          249 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADMFL010000307         1057 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.315, whole genome shotgun sequence.
ACCESSION   DADMFL010000307 DADMFL010000000
VERSION     DADMFL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.315"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..1027
                     /locus_tag="JNZ27_004338"
                     /pseudo
     CDS             1..1027
                     /locus_tag="JNZ27_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808853.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          249 a          236 c          273 g          299 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaccaaccg acagcaaaac ctgcacctgc
      361 tggtagcatg ttccagagca aatccttctg gctggcaaca gggctgcgtt ttggtcaggc
      421 gggtaactcc ggtttaattc agactttcct tgcaggctat ttagtacaga cgttattgtt
      481 taacaaagca attccaacag atgcattgat gatcagttcg attctcggct ttatgaccat
      541 tccgttcctt ggttggttat ccgataaaat tggtcgccgg atcccgtata ttattatgaa
      601 tacctctgcg attgtgctgg cgtggccaat gctttctatc atcgtagata aaagctatgc
      661 cccgagcacc attatggttg cactgattgt gattcataac tgtgcggtgc tgggattatt
      721 tgctctggaa aacattacca tggcagaaat gttcggcagt aaaaaccgct ttacccggat
      781 ggctatttca aaagaaattg gtggtcttat cgcttccggt tttggtccta tcctggcggg
      841 tattttctgc accatgacgg aatcctggta tccgatcgcc attatgatca tggcatattc
      901 agtgattggt ttaatctctg cgctgaaaat gccagaggtg aaagaccgtg atttaagtgc
      961 gctggaagac gctgcggaag atcaaccgcg tgttgtaaga gctgcgcaac cttccagaag
     1021 tctgtaaacc ctcaatccct tctcttaccg gagaggg
//
LOCUS       DADMFL010000308         1038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.316, whole genome shotgun sequence.
ACCESSION   DADMFL010000308 DADMFL010000000
VERSION     DADMFL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.316"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..767
                     /locus_tag="JNZ27_004339"
     CDS             <1..767
                     /locus_tag="JNZ27_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8666374.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..>1038
                     /locus_tag="JNZ27_004340"
     CDS             788..>1038
                     /locus_tag="JNZ27_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8666375.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     CWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          337 a          228 c          235 g          238 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga atttattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacgat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaat gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt
     1021 actgacttac ctgcaaag
//
LOCUS       DADMFL010000309         1016 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.317, whole genome shotgun sequence.
ACCESSION   DADMFL010000309 DADMFL010000000
VERSION     DADMFL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.317"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..272
                     /locus_tag="JNZ27_004341"
     CDS             <1..272
                     /locus_tag="JNZ27_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8666376.1"
                     /translation="RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHA
                     DTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            274..>1016
                     /locus_tag="JNZ27_004342"
     CDS             274..>1016
                     /locus_tag="JNZ27_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666377.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPREEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          186 c          200 g          307 t
ORIGIN      
        1 tccgtagaaa tgatggttcg atccgcttta acaataagat gccttatcac gttacgctgg
       61 gttatatcgg aacaaacgga atcacaatgt tgccacaaac gcaaagttta atggtaaccc
      121 cttatagcca tgcagatacg cagtttaaaa atgttccgtc agcttttcag gttggttata
      181 tcaatgattt tggtggatta agtttttatg aaataaactg cccgacggtt aataactctt
      241 gtaatgtttc cgtagccaaa cgagataaat aatatgcgct acttattaat tgtaattata
      301 tttattatag gttttagtgc attacctgta tgggcaatgg actgctatgc tgaacatgaa
      361 gagggaaata ccgtagtcat tggttacgta ccaagaattt ctatccccag taatggtaaa
      421 aaaggtgata agatctggca aagcagtgag tattttatga atgtcttctg taataatgca
      481 ttacctgccc catcacctag agaagaatac ccatcagcct ggactaatat aatgatgttt
      541 ttaccaggcg gtcaggactt ttataatcag aactcctata tttttggcgt aacatacaat
      601 ggagttgatt atgattcgac tgcccctaat gctatcgcag caccagaatg cattgatata
      661 aaaggagcgg gtacctttaa taatcactat aaaaaccccg ctgtctgtag tggaggtcct
      721 gagcctcaat tatcagtaac ctttccagct cgcgtacaac tctatattaa attagcaaaa
      781 aatgccaata gagttaataa aaacctggtg ttacctgatg aatatatcgc tccagagttt
      841 aaaggtatga gtggcgcagg agctatagaa gtagacaaaa atttgacctt caggattcgt
      901 ggattaaata atattcatgt ccttgactgc tttgttaatg tcgcactgga accagctgat
      961 ggcgttgttg actttggtaa aataaattcc cgaacaatta aaaataccac cgttag
//
LOCUS       DADMFL010000310          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.318, whole genome shotgun sequence.
ACCESSION   DADMFL010000310 DADMFL010000000
VERSION     DADMFL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.318"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNZ27_004343"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNZ27_004343"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY8666378.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          221 g          283 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADMFL010000311          976 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.319, whole genome shotgun sequence.
ACCESSION   DADMFL010000311 DADMFL010000000
VERSION     DADMFL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.319"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            86..>976
                     /locus_tag="JNZ27_004344"
     CDS             86..>976
                     /locus_tag="JNZ27_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY8666379.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTMYNDVLFPG"
BASE COUNT          258 a          230 c          241 g          247 t
ORIGIN      
        1 ttgtttactg tgcgctgttg tccatgtgat attgtgtacc agatgtgtgc caaactatta
       61 tttctgagcg taccaaatat tatttatggc tataagcgac acaaaactgc gtactattta
      121 tggtaaacca tattcgggcc cacaagaagt ggctgatgcc gatggcctca gcgtacgaat
      181 ttcaccgaag ggggtcatcc agttccagta ccgctatcgc tggcatggca agcctaatcg
      241 acttgggctt ggtcgatacc catccctgtc tttgaaggat gccagacaga tcactgctga
      301 cttgcgaaag ctctatttct caggaacgga tccacgcacc tattttgaag agaaggtgga
      361 gaactccatg acggtcgccc agtgtctcga ctactggttc gacaactacg tctctacaac
      421 tctcagagaa aaggcccagg cactttatcg atcaacggtt atgaagcgca tgcatgacgc
      481 cttccctaat cgtccggcat cttctatcac ggttaagcaa tgggttgacc tgcttaccga
      541 agaagaaaaa gataatccac gccgagcaag gcaggtgcta agtcaactaa gatcagcaat
      601 tagttggtgc atgcggcgtc agttgataga tagttgcgca attatgagca tccagccaag
      661 ggacttcggc tcccgcgctg aggtagggga tcgggtactg tcgtatcacg aactggctaa
      721 gatttggctt gctattgaaa gaagccgtgc gtctacgtca aataagctcc ttcatcagat
      781 gcttatgctg tggggggcga ggctctcaga gcttaggctg gcaacaaaga cagaatttga
      841 cctgctggac aacgtatgga ccgtaccgaa agagcatagc aagatgggta atgttatccg
      901 ccgtccaatc ttcgaacaaa ttaagccttt tctcgaaaag gccatgacaa tgtacaatga
      961 tgttcttttc cctgga
//
LOCUS       DADMFL010000312          958 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.320, whole genome shotgun sequence.
ACCESSION   DADMFL010000312 DADMFL010000000
VERSION     DADMFL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.320"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            97..>958
                     /locus_tag="JNZ27_004345"
     CDS             97..>958
                     /locus_tag="JNZ27_004345"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HAY8666380.1"
                     /translation="MPSTQQEVLRLSRDVEAGRAVYLQLLNRQQELSISKSSAIGNVR
                     IIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLRRGVEAPEQLEEHGISV
                     YSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPADSAVEAVRALRTSLHF
                     AMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFIDADLRRGYSHNLFTVS
                     NEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSELLMRDRMRQLLEWANDH
                     YDLVIVDTPPM"
BASE COUNT          240 a          226 c          257 g          235 t
ORIGIN      
        1 ctgtataaga aagatcaccc aacttatcgt gcgctgctgg aaaaacgcca gacgctggag
       61 caagaacgca aacgcctgaa taagcgggta tcggcaatgc cttccaccca acaggaagtg
      121 ttgcgtttaa gtcgtgacgt agaagcgggc cgtgcggtat atctgcaatt acttaaccgc
      181 cagcaggagt tgagtatttc gaaatccagt gccattggta acgtgcggat tatcgacccg
      241 gcagtcactc agccgcagcc agtgaaaccg aaaaaagcgt tgaatgtggt gcttggtttt
      301 attcttggcc tgtttatttc ggtgagtgcc gtgctggcgc gtgcgatgtt gcgtcgtggt
      361 gtagaagccc cggaacaact ggaagagcac ggcatcagcg tttattccac tatcccaatg
      421 tccgagtggc tggataaacg cacccgtctg cgtaagaaaa atttattttc taatcagcag
      481 cgccatcgta ctaaaaatat ccccttcctg gcggtggata acccggcgga ttctgctgtg
      541 gaagccgtac gtgcgctacg aaccagtctg catttcgcta tgatggagac tgagaataac
      601 attctgatga tcaccggtgc gacgccagac agtggtaaaa cgtttgtcag ttcaactctg
      661 gcagcggtga tcgcccagtc cgatcaaaaa gtgttattta ttgatgccga cttacgccgt
      721 ggttattcgc ataacctgtt taccgtgagt aatgaacatg gcttgtcgga atatctggca
      781 ggtaaagatg agctcaacaa agtgatccag cattttggca aaggaggctt tgatgtgatt
      841 actcgcggtc aggtgccacc taacccgtct gaactgctga tgcgcgatcg gatgcgtcaa
      901 ttactggaat gggcgaacga ccattacgat ctggtgattg tcgatacgcc gccgatgc
//
LOCUS       DADMFL010000313          915 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.321, whole genome shotgun sequence.
ACCESSION   DADMFL010000313 DADMFL010000000
VERSION     DADMFL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.321"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..212)
                     /locus_tag="JNZ27_004346"
     CDS             complement(<1..212)
                     /locus_tag="JNZ27_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666381.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRA"
     gene            complement(212..915)
                     /locus_tag="JNZ27_004347"
                     /pseudo
     CDS             complement(212..915)
                     /locus_tag="JNZ27_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          207 a          300 c          194 g          214 t
ORIGIN      
        1 aatgcgcgga cgccaccgtg ccgttccaga aaggctttta aagatgggtg ttcaccttta
       61 cgaacacgta tcgtcacgct gtaagtcgac aactggttat tcagtcccgc cgcacgacgc
      121 tgttcataac catcgcccag cttcactgtc accactttcg gctctgatac cacattcata
      181 tccgggcgca ctttccagtg aaacgtctcc attaccggta tgctccactt aaccggccac
      241 catcacgggc ctgctgttgc ataaagtccg cggccgcttt tttcccgagg tcataaacca
      301 ccttcagggc agccggacct atctgcccgt tcgtgccgtc gttattgatc tcaatgttgt
      361 actgcggggc aaacatcacc atgcctgaac caccagtatc cgccacaacc ccccagctta
      421 ccatcagcac cgcgacgcag tggcagaatg gcttcaggtc ccgcttcccc catcacaccc
      481 gcgccttttg caaaagcaaa aaacgtcgga cggttaacca ccgtgccact gtagcgactc
      541 aaatcagcag actggtaaac accgccatca gcattagtcg tgaagccgaa taaagaaccg
      601 acgcccttta ccgcctgcat catggccatc tgtgccataa ttctggccat atctgacaac
      661 agggaagagg tgaaggattt aaaattcagc ttaccggtgg tacagaatgt cgccagtgca
      721 ttaccggcac tgctgaatgc cactgtaaac atctgttctg ccgttcccgc cgcattatcc
      781 gcatccgccg taaaattctg aaacgcccgc atggcaccgt ttttccagtc gccctgcgcc
      841 acttccagtt cctgccagta gcgtttgttt tcattcagtt gtcgcttcag gctccccttc
      901 agcacctcct cagcc
//
LOCUS       DADMFL010000314          910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.322, whole genome shotgun sequence.
ACCESSION   DADMFL010000314 DADMFL010000000
VERSION     DADMFL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.322"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="JNZ27_004348"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="JNZ27_004348"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY8666382.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICALPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYTS"
BASE COUNT          248 a          221 c          212 g          229 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc actcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac acgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat
//
LOCUS       DADMFL010000315          908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.323, whole genome shotgun sequence.
ACCESSION   DADMFL010000315 DADMFL010000000
VERSION     DADMFL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.323"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            6..755
                     /locus_tag="JNZ27_004349"
     CDS             6..755
                     /locus_tag="JNZ27_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8666383.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          252 a          195 c          201 g          260 t
ORIGIN      
        1 gttatatgaa aagacagctt tttactctta gtattgtcgg ggtattttct ttaaatacct
       61 ttgcatcatt ccctccggga aatgatgtga caacaaaacc tgacctttac tacctgacaa
      121 acgataatgc tattgacagt ctggcattat taccgcctcc accacaaatc ggaagtattg
      181 ccttcctgaa tgaccaggcc atgtatgaaa aggggcgttt attgcgaaat actgaaagag
      241 ggaagctggc agctgaggat gccaatctca gtagtggtgg cgtggcaaat gtattttctg
      301 ctgcttttgg ttctccgata acggctaaag attcaccaga attgcataaa ttactgacaa
      361 atatgattga ggatgccgga gacctggcaa cgcgttcagc aaaagaatat tatatgcgta
      421 ttcgaccttt tgctttttat ggtgtttcta cgtgtaatac aaaagaacag gatacattat
      481 ccagaaatgg ctcttatcca tcaggtcata catcgattgg ttgggcaaca gcacttgtat
      541 tgtccgaaat aaatccggca cgccaggata ccattctgaa acggggttat gaactggggg
      601 acagcagggt tatttgcggt tatcactggc aaagtgatgt tgacgcagca cgtattgttg
      661 gctctgctat tgtagcaaca ctgcactcaa atcctgtgtt ccaggcacaa ttacaaaaag
      721 cgaaagatga attcgctaac aatcagaaaa aataatgctt ttagccagta cgccttataa
      781 ccccgacctt cagggctaat gccagtcagt taagcaactg actggctctt tttcagggct
      841 gtggggtatt tccagggcct ctcctttacc actctcggga aggccctttc ccttcttgtc
      901 ggtaattt
//
LOCUS       DADMFL010000316          895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.324, whole genome shotgun sequence.
ACCESSION   DADMFL010000316 DADMFL010000000
VERSION     DADMFL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.324"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(110..439)
                     /locus_tag="JNZ27_004350"
     CDS             complement(110..439)
                     /locus_tag="JNZ27_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY8666384.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>895)
                     /locus_tag="JNZ27_004351"
     CDS             complement(611..>895)
                     /locus_tag="JNZ27_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666385.1"
                     /translation="HFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          217 a          207 c          200 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatg
//
LOCUS       DADMFL010000317          889 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.325, whole genome shotgun sequence.
ACCESSION   DADMFL010000317 DADMFL010000000
VERSION     DADMFL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.325"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..680)
                     /locus_tag="JNZ27_004352"
     CDS             complement(<1..680)
                     /locus_tag="JNZ27_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8666386.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          264 a          146 c          185 g          294 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aactctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata tcctgataaa atgtgatact
      721 ggggaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagtgatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcata
//
LOCUS       DADMFL010000318          879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.326, whole genome shotgun sequence.
ACCESSION   DADMFL010000318 DADMFL010000000
VERSION     DADMFL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.326"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..700
                     /locus_tag="JNZ27_004353"
     CDS             <1..700
                     /locus_tag="JNZ27_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8666387.1"
                     /translation="SWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLSP
                     EDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYAC
                     KGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQE
                     VHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSSY
                     PSIEQFNEFLTLNE"
BASE COUNT          259 a          159 c          188 g          273 t
ORIGIN      
        1 atcatgggga attaatacca cttatgttga gaaggtcccc tgtaccagca gtggtgttgc
       61 accgatcttt gttaatgcca actcgagcaa tagtatatta ataattaagg gagcgaataa
      121 atttctttcc ccggaagata tcgatcgagc tgcagaagat ctgaaaaaat gcaaattgat
      181 cgttttacaa ctggaagttc aactggaaac agtttaccac gcgattgagt ttggtaagaa
      241 aaatggtatt gaagtgttat taaatccggc tccggcattg cgtgagttag atatgtctta
      301 tgcctgcaaa ggtgatttct ttatcccgaa tgaaacagaa ctggaaatat taaccggtat
      361 gtcggttgat acttatgatc atattcgttt ggccgcacgt tcgttagttg ataaagggct
      421 gaacaatatt attgttacca tgagcgaaaa aggcgcatta tggatgaccc gcgatcagga
      481 agtccatgtt cctgcattca aagtaaatgc tgttgatact agtggcgctg gtgacgcatt
      541 tattggttgc ttttcccatt attatgttca aagcggtgat gttgaagccg ctttgaaaaa
      601 agccgctctc tttgccgctt tcagcgtcac agggaaaggt atgcaatcat cttatccgag
      661 tattgaacaa tttaatgaat ttttaacctt aaacgaataa tcatcctgca cagttaaagg
      721 tagcactatg aacgataaaa acatcattca gatgcctgat gggtatctga ataagactcc
      781 cttgttccag tttattttgt tatcgtgttt attcccgcta tggggatgcg cagctgcatt
      841 aaatgacata ttaatcactc aattcaaaag tgtattttc
//
LOCUS       DADMFL010000319          877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.327, whole genome shotgun sequence.
ACCESSION   DADMFL010000319 DADMFL010000000
VERSION     DADMFL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.327"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>877
                     /locus_tag="JNZ27_004354"
     CDS             <1..>877
                     /locus_tag="JNZ27_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8666388.1"
                     /translation="HVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFT
                     LPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQ
                     RRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRY
                     LDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGM
                     DKPEPVAKVCYAQMVK"
BASE COUNT          206 a          219 c          284 g          168 t
ORIGIN      
        1 ccacgtcaga tacgtcacta actcatgcgg cagccgtgcc tgcccgtcct gcggaaaaaa
       61 ggccacagac ctgtggatag cgacacagct gaatcgtctt cctgactgcg actgggtaca
      121 cctggtcttc accctgccgg acacgctgtg gccggtgttc gaaagcaacc ggtggctgct
      181 gaatgacgtg tgccgtctgg cggtggagaa tctgctgtat gccgcccgga aacgggggca
      241 ggaacccggt atcttctgcg ccatccacac gtatggccgt cgtctcaact ggcatccgca
      301 tgtacatgtg tctgtaacct gtggaggtct gaataagcat ggtcagtgga aaaagctgag
      361 cttcctgaaa gacgcgatgc gttcgcggtg gatgtggaat atgcggcaac ggcttctgaa
      421 agcgtggtca gaggggctgg cgatgccgga gtcgttgtca catatcacga cggaatcaca
      481 gaggagaagc ctggtgctga aagccggagg aaaatactgg catgtgtaca tgtcgaaaaa
      541 aacggccggc gggaggaata cggcgcgcta cctgggtcgt tatctgaaga agccgccgat
      601 agcggcctcc cggctggcac attacaacgg aggggcgagc ctgagcttcc gttacctgga
      661 ccacaaaacg ggagaaacgg cgacggaaac gctgacacag cgtgagctgg tcgcgaggct
      721 gaaacagcac atcccggaga agttttttaa gatggtgagg tacttcgggt tccttgccag
      781 ccgggtgtgt ggagagaagc tgccgcaggt gtaccgtgct ctggggatgg ataaaccgga
      841 accagtggcg aaagtgtgct atgcacaaat ggtgaaa
//
LOCUS       DADMFL010000320          858 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.328, whole genome shotgun sequence.
ACCESSION   DADMFL010000320 DADMFL010000000
VERSION     DADMFL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.328"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..213
                     /locus_tag="JNZ27_004355"
     CDS             <1..213
                     /locus_tag="JNZ27_004355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666389.1"
                     /translation="CADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIP
                     AGINADGMYCPECGTVHWPDGVIPPF"
     gene            261..690
                     /locus_tag="JNZ27_004356"
                     /pseudo
     CDS             261..690
                     /locus_tag="JNZ27_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          213 a          171 c          268 g          206 t
ORIGIN      
        1 tgcgcagacc tgaatattcc gttctttgtg gatgccagaa acaaaaaact ggtcttctgg
       61 tttgtacgtg ttgatgacga agggtatccg gaaattgccc gctgcatgga gcgggaattt
      121 gcgacaattc ctgccggtat caacgccgac ggcatgtatt gccctgaatg cggtacggtt
      181 cactggccgg atggcgttat tccgcctttc tgatgcttcc ccgttttgcc gatatttttc
      241 agcagggaaa ccgatggctt aactggctgg agaagcagcc ggtgcagatg agccgcctgg
      301 aacattatgc cggtcaggat gaaattggcc tgcgttacaa cagccaccgg acaaaacgag
      361 aagaaaacct tgtgatgagt ggcgacgagt ttatggaaag gttctcctgg catgtggcgg
      421 ataaggggtt ccggatggtg atacggggtc ctgagtccgg tgaagcggcg attactggaa
      481 gatgtggtgt acgccataac ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga
      541 gggatgtatc agcagtgact gaaggttgac ccgctgaagt gcgagctgcg cggaggtcag
      601 atgcggttta cggggctgaa gaggggttac cgtctggcag agcaggttat gatgcatgag
      661 ccgctggctc gaatacggtg gtgcggctga gagtcgcata ggggaagttg cgtccatgtt
      721 gaggggaacg gagcaaaaaa ccatcagaga tacattgaat caatcaatgt aagcaatatt
      781 aagtgtctat accgattact tgatgatcag gttgtgttga ataaacgcgc attcttcggt
      841 gcttttttaa tcttttgg
//
LOCUS       DADMFL010000321          853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.329, whole genome shotgun sequence.
ACCESSION   DADMFL010000321 DADMFL010000000
VERSION     DADMFL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.329"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..761
                     /locus_tag="JNZ27_004357"
                     /pseudo
     CDS             1..761
                     /locus_tag="JNZ27_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          231 a          184 c          203 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DADMFL010000322          852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.330, whole genome shotgun sequence.
ACCESSION   DADMFL010000322 DADMFL010000000
VERSION     DADMFL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.330"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            60..557
                     /locus_tag="JNZ27_004358"
                     /pseudo
     CDS             60..557
                     /locus_tag="JNZ27_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            598..789
                     /locus_tag="JNZ27_004359"
                     /pseudo
     CDS             598..789
                     /locus_tag="JNZ27_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          220 a          210 c          250 g          172 t
ORIGIN      
        1 agcgccagcc ccgtcccgcc cgatagagcg cggcatagcg ggccccggat tacttgcgtg
       61 cgacggcaga agaagccctg aagcgaatca gtgaactgta cgccatcgag gatgaaatac
      121 ggggattacc agagtcagag tgtcttgcag tcaggcagca gcgaagcaaa gcgttactga
      181 cgtcgctgca tgaatggatg gtggagaaga atggcacgct gtcgaaaaaa tccagactgg
      241 gcgaagcatt cagctatgta ctgaatcagt gggacgccct ctgttattac agtgatgacg
      301 gtctggcgga ggcggacaat aacacagcgg aaagagcgct tcgtgcagtc tgtctcggaa
      361 agaaaaatta cgtgttcttc ggtagcgatc acggcggcga gcgtggtgca ctgctgtacg
      421 ggctgatcgg cacctgccgt ctgaacggta tcgatccgga agcgtatctg cgccatattc
      481 tgagcgtact gccggaatgg ccttccaacc gagttgacga cctcctgcca tggaaggtgg
      541 tgctaccatc aggataatcg tcaatacggt gttcggtgaa cgtttacttg gagcatcatg
      601 cttcatgaag aaaaactggc acgtcatcaa cgtaaacagg cgatgtatac ccgaatggca
      661 gccttcccgg cggtgaaaat gttcgaagag tatgacttca cgttcgccac cggagcaccg
      721 cagaagcagc tccagtcgtt acgctcactc agcttcatag agcgcaatga aaacatagtc
      781 ttactgggga catctgacat cacaaatccc cgggtgggga tttgtgtata agagacagcc
      841 catggaggaa gg
//
LOCUS       DADMFL010000323          830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.331, whole genome shotgun sequence.
ACCESSION   DADMFL010000323 DADMFL010000000
VERSION     DADMFL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.331"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..326
                     /locus_tag="JNZ27_004360"
     CDS             <1..326
                     /locus_tag="JNZ27_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY8666390.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNZ27_004361"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JNZ27_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY8666391.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          182 c          155 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gcggggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADMFL010000324          787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.332, whole genome shotgun sequence.
ACCESSION   DADMFL010000324 DADMFL010000000
VERSION     DADMFL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.332"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..200
                     /locus_tag="JNZ27_004362"
     CDS             <1..200
                     /locus_tag="JNZ27_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666392.1"
                     /translation="PTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKL
                     DEGSNTASGLGKKYWTEICVR"
     gene            188..355
                     /locus_tag="JNZ27_004363"
     CDS             188..355
                     /locus_tag="JNZ27_004363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8666393.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            368..733
                     /locus_tag="JNZ27_004364"
                     /pseudo
     CDS             368..733
                     /locus_tag="JNZ27_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          224 a          167 c          193 g          203 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat ggagggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      781 ggtgctt
//
LOCUS       DADMFL010000325          779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.333, whole genome shotgun sequence.
ACCESSION   DADMFL010000325 DADMFL010000000
VERSION     DADMFL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.333"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..546)
                     /locus_tag="JNZ27_004365"
     CDS             complement(<1..546)
                     /locus_tag="JNZ27_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666394.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
BASE COUNT          229 a          149 c          157 g          244 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcga
//
LOCUS       DADMFL010000326          764 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.334, whole genome shotgun sequence.
ACCESSION   DADMFL010000326 DADMFL010000000
VERSION     DADMFL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.334"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            169..>764
                     /locus_tag="JNZ27_004366"
     CDS             169..>764
                     /locus_tag="JNZ27_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY8666395.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRG"
BASE COUNT          196 a          200 c          203 g          165 t
ORIGIN      
        1 gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa ttaagggaca
       61 cagcctaaat agctgcgcgg aatagtagat ccctttgagg gaacttagcc cggattgtgc
      121 gatctgatca atcgccaaat caaaacaaat caccaaccgg actgagcaat gccgatcata
      181 gcaccaattt cccgtgacga acgacgcctg atgcagaaag ccatccataa aacacacgat
      241 aaaaattatg cccgcagact gactgccatg ctgatgctgc accggggcga ccgtgtcagc
      301 gacgttgcca gaacgctctg ctgcgcccgt tcctcggttg gacgctggat taactggttc
      361 acgcagtcgg gtgttgaggg actgaaatca ttacctgccg ggcgtgcccg tcgctggccg
      421 tttgagcata tctgcacact gttacgtgag ctggtaaaac attctcccgg cgactttggc
      481 taccagcgtt cacgctggag tacagaactg ctggcaataa aaatcaatga gataaccggt
      541 tgccagttaa atgccggaac cgttcgccgc tggttgccgt ctgcggggat tgtgtggcga
      601 agggctgcgc caactctgcg tatccgtgac ccgcataaag atgaaaagat ggcagcaatc
      661 cataaagcac tggacgaatg cagcgcagag catccggtct tttatgaaga tgaagtggat
      721 atccatcttc atcccaaaat cggtgcggac tggcaactgc gcgg
//
LOCUS       DADMFL010000327          736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.335, whole genome shotgun sequence.
ACCESSION   DADMFL010000327 DADMFL010000000
VERSION     DADMFL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.335"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(229..735)
                     /locus_tag="JNZ27_004367"
     CDS             complement(229..735)
                     /locus_tag="JNZ27_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY8666396.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          200 a          183 c          160 g          193 t
ORIGIN      
        1 accaactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
       61 tcatctgacc accagcgcgt ttaacgccct gtgccatttt tcaggcacaa aaaaacccgc
      121 ataaagcggg ttctttcaag tgtccatgtc tgctattcgc ctcgcgatac agctttgcga
      181 agcttaccgg aatttaagca gtttatgtgc aaaaatgcaa gaactttttt aaagctgcat
      241 caacctttcc accagtttat ctctgcgaac aacaaaccaa ccattggctc tcgccagttc
      301 cagccatgac tcaagggaaa taacaatatc atcatcccgt aactgaattg tggaaacagt
      361 gacaccgcct ctctgataac agagaactcg cgtgtcgtaa cttttctggc atgaaactgg
      421 cgttgacgga tccctttgac tgaaatagca gtcttccagt ttttcgaaca catcccacgc
      481 ctgatcggtt tcgagcattt tggcgtggcg tgctgcgcct cgttctgtcc agaggatgag
      541 ggagcgggct ttgggtgaaa ctggattttg tgagtagttt aaagcgaccc gcaattcttt
      601 aaggtcatta ccaacaactt tgaaaaagtg tttcccttca acgaagcgta ctttgttctc
      661 atgatgattc tggcgaatac gcaccggctc agtgccgtaa agctgcgcca aaagttcggt
      721 ggtaataaca ggaatc
//
LOCUS       DADMFL010000328          727 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.336, whole genome shotgun sequence.
ACCESSION   DADMFL010000328 DADMFL010000000
VERSION     DADMFL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.336"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..485)
                     /locus_tag="JNZ27_004368"
     CDS             complement(<1..485)
                     /locus_tag="JNZ27_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8666397.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     V"
BASE COUNT          222 a          180 c          135 g          190 t
ORIGIN      
        1 aagacctgat gcaaatcaac gtccttttgc cattcataaa catcatctgg ataatcggca
       61 tgagcaataa catgcttgcc ggtgcgactg tgtacttcaa gaataaaggg caaatgctga
      121 catttttcca gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc aataaaccag
      181 tcgccgccat tcatcaacca caaagacatc tgctggaatg ccagcgcatc catcgccatc
      241 tgttcatgat tgcctcttac cgcacgaacc caatgttgtt ccagtaactg cagacaacgt
      301 aaactttgcg gcccacgatc gataacgtct cccactgaga taagtaaatc tcgccacgga
      361 tcaaaacgac aatgccataa tttgcggcgc aactgctcaa gacaaccgtg tatatcgcca
      421 gaaagccaga tatgtcgcca ttgatgaccc gcaattctct gataaacggg cgcaggctgt
      481 ttcatcaata ttttcctccc gcgctaaaga tcacataatc ttaacaagaa tgttaaaaaa
      541 cgctggactc agacagtaga gtgtgtgtta tggttgacta taaagtcagc gaaggaaatg
      601 cttctggctt ttaacagata aaaagagacc gaacatgatt cctgttttcg tcaacaaaca
      661 ataaaaccct ttaaaattaa tgcgttgaat aaatttacgt tcatctttta atccctgaca
      721 cgtacca
//
LOCUS       DADMFL010000329          727 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.337, whole genome shotgun sequence.
ACCESSION   DADMFL010000329 DADMFL010000000
VERSION     DADMFL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.337"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(54..605)
                     /locus_tag="JNZ27_004369"
     CDS             complement(54..605)
                     /locus_tag="JNZ27_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAY8666398.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQPDYKIAYE"
BASE COUNT          210 a          147 c          141 g          229 t
ORIGIN      
        1 cacttatcat taatccgtta atgatgtggc cgggcgaccc cggcctaatg tgattactcg
       61 taagcaatct tatagtcagg ctgagcgtaa acgttgcccg gggtagcatc tttgcccggg
      121 gattccatac gagctttgaa gttcagttcc tgatagctgt tagtattgtt gaatgtaatt
      181 actaccggag tacctaaagt cacttcgccg ctctggctat ccagaatacg tacgccaacg
      241 cctgtggcac caccgatttc agtttctttg gtgtttttca gcaggtcagt ctgacccgtc
      301 atagtattcg ctgtggtgaa agtaaccttc gcttttgaat aagtggttcc accctggctc
      361 aacatgcagt cttgcaggtg aatagtgaaa ggttcagcca aagatttctg atcgcttttc
      421 agtacggtat ctgctacttc acccagattt acagttaaat cttcgtcacc tggtttaata
      481 gaacatggag cagagataac ttcaccttta aatttaacaa taccgtgacc ttcatcagca
      541 aacacgttat tggaaagaat cattgctaca ccagcaacaa gaagtgactt tttaaaagca
      601 gacataatat tcctttatat aaagataagt aaaaaagaga atgattagta actgttattt
      661 aaggactacc aatcattaaa atattatatt ttgttttgtg atttttataa aattagttta
      721 tctaaat
//
LOCUS       DADMFL010000330          713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.338, whole genome shotgun sequence.
ACCESSION   DADMFL010000330 DADMFL010000000
VERSION     DADMFL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.338"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..85
                     /locus_tag="JNZ27_004370"
     CDS             <1..85
                     /locus_tag="JNZ27_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666399.1"
                     /translation="ASNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            85..540
                     /locus_tag="JNZ27_004371"
     CDS             85..540
                     /locus_tag="JNZ27_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY8666400.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            543..>713
                     /locus_tag="JNZ27_004372"
     CDS             543..>713
                     /locus_tag="JNZ27_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8666401.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNL"
BASE COUNT          208 a          171 c          182 g          152 t
ORIGIN      
        1 tgcctcaaat ggcagtacgt taactattca gtcaaatggt gcggcgttta ctgccacaac
       61 catcaagata atcgcggagc agtgatgatt cagttcaaac caacgcgaaa catcgacctg
      121 atcgaagcag taggaaatca cactgacatt atcgccggga gcaacaacgg cgatggatac
      181 gactacaagc ctgaatgccg ttactttgag gttaacgtgc acgggcagtt cggcggaatt
      241 gtttactatc aggagattca gccgctaacc tttgattgcc acgccatgta cctgccagag
      301 attcgtggat tcagcaagga aatcgggctg gcgttctggc gatacattct gactaacacc
      361 accgttcagt gcgtcacatc gttcgctgcg cgcaaattcc gccacggtca gatgtactgc
      421 gcaatgattg gccttaagcg tgtaggaacc atcaagaaat acttcaaagg cgtggatgac
      481 gtgacgtttt acagcgccac acgcgaagaa ctaatcgact tcctgaatca cgggagatag
      541 ccatgttata tgcatttaag ctgggcagaa aactgcgcgg cgaggaacct tattgccctg
      601 aaaaaggcgg gaaaggtggc agctctgata aaagcgcaaa gtatgcagca gaagctcaga
      661 agtatgccgc agacctgcaa aatcagcagt ggcagacgat catgaaaaac ctt
//
LOCUS       DADMFL010000331          711 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.339, whole genome shotgun sequence.
ACCESSION   DADMFL010000331 DADMFL010000000
VERSION     DADMFL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.339"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..165
                     /locus_tag="JNZ27_004373"
     CDS             <1..165
                     /locus_tag="JNZ27_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666402.1"
                     /translation="DGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGSAWQHP
                     KRTERPGIRI"
     gene            185..>711
                     /locus_tag="JNZ27_004374"
     CDS             185..>711
                     /locus_tag="JNZ27_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8666403.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRP"
BASE COUNT          204 a          187 c          188 g          132 t
ORIGIN      
        1 gacgggttat gcctgttcgc caaacgcctg gaacggggcc gcttcgtctg gccggtgacc
       61 cgggaaggga aagtgcacct gacgccagct cagttatcca tgctactgga ggggagcgcg
      121 tggcaacatc ccaaacggac agaacggcct ggcatccgga tataacccgt gataaaacaa
      181 gggaatgaac aatgaactcc ccgatgatat tgagctgctt aaagccatgt tgcgtaagca
      241 acagagtcgg cttcgacagt atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg
      301 gctgaaagcg caactcgaca ggttgcgtcg tatgttgttc ggccagagtt cagagaaaaa
      361 gcgtcataag cttgaaaatc agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa
      421 ccggctgaac acagccagaa atcttctgga agatgcatcg tcagtcacag attcacctga
      481 caccagtccc ccgtcagaaa acccgatcgc cagtaagcct gaatccccgg gacgaaaatc
      541 ttcacgaaaa ccgctgccgg cagaacttcc ccgggagaca catcgccttc tgcctgctga
      601 aaccagttgc ccggcctgtg gaggtgttct gaaagaaatg ggggaaacaa tctcagagca
      661 actggatatc attaataccg cctttaaagt tatcgaaacc atacgtccca a
//
LOCUS       DADMFL010000332          700 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.340, whole genome shotgun sequence.
ACCESSION   DADMFL010000332 DADMFL010000000
VERSION     DADMFL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.340"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            508..>700
                     /locus_tag="JNZ27_004375"
     CDS             508..>700
                     /locus_tag="JNZ27_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666404.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLR"
BASE COUNT          252 a          123 c          142 g          183 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa atgtattacg cgtaacaaac tcatcttctt ctggcataag tgaaaaacat
      661 ttagatcact gtgctaatac tgtgaaaaac tttttaagga
//
LOCUS       DADMFL010000333          696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.341, whole genome shotgun sequence.
ACCESSION   DADMFL010000333 DADMFL010000000
VERSION     DADMFL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.341"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..452
                     /locus_tag="JNZ27_004376"
     CDS             <1..452
                     /locus_tag="JNZ27_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8666405.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..>696
                     /locus_tag="JNZ27_004377"
     CDS             455..>696
                     /locus_tag="JNZ27_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8666406.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVP"
BASE COUNT          205 a          128 c          175 g          188 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctga
//
LOCUS       DADMFL010000334          667 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.342, whole genome shotgun sequence.
ACCESSION   DADMFL010000334 DADMFL010000000
VERSION     DADMFL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.342"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(351..>667)
                     /locus_tag="JNZ27_004378"
     CDS             complement(351..>667)
                     /locus_tag="JNZ27_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY8666407.1"
                     /translation="KKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRF
                     EI"
BASE COUNT          187 a          140 c          112 g          228 t
ORIGIN      
        1 tttttctgct cattattggc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcactc ccgtaaagac aggagtcaag ttcccgctcc gacataactg
      121 cgtgcaatgg attttgaagg aaccgtcagc ccattccccg gaggcaaaca tgggctggca
      181 aaagtgtagc ggtattaggc acagctattt aggaaagagg gaccatctcc atctcattaa
      241 atggaccacg ccagacaatt cactgtcatg ctgatgctgc actgtgggga cagaatcact
      301 catgatgact tcgataatcg acgacattat tatttggccg agcttttagg tcatatctca
      361 aatcgtttgc ctaatattgc tccatttttt aataaaaaat caatcatttt tgaatctttg
      421 cttttcattg cattatcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt
      481 acatcaacca atccattatt gataaaatat tctagcactt tatagttggc accatcttta
      541 ctaagtaagt attcaacatc atacagatta caatatctat tgttgttcat tttctccatc
      601 tctgcgacat cttgttttgt aaaattaaat ttagatagta aataaagtgc tatatcagct
      661 tttttat
//
LOCUS       DADMFL010000335          657 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.343, whole genome shotgun sequence.
ACCESSION   DADMFL010000335 DADMFL010000000
VERSION     DADMFL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.343"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            101..>657
                     /locus_tag="JNZ27_004379"
     CDS             101..>657
                     /locus_tag="JNZ27_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8666408.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          108 g          192 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tttggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatctaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttaccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat agtttctgga aacagattag acagcat
//
LOCUS       DADMFL010000336          651 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.344, whole genome shotgun sequence.
ACCESSION   DADMFL010000336 DADMFL010000000
VERSION     DADMFL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.344"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..221)
                     /locus_tag="JNZ27_004380"
     rRNA            complement(<1..221)
                     /locus_tag="JNZ27_004380"
                     /product="16S ribosomal RNA"
BASE COUNT          153 a          167 c          139 g          192 t
ORIGIN      
        1 gcaagaggcc cgaaggtccc cctctttggt cttgcgacgt tatgcggtat tagctaccgt
       61 ttccagtagt tatccccctc catcgggcag tttcccagac attactcacc cgtccgccac
      121 tcatcagcga aacagcaagc tgtttcctgt taccgttcga cttgcatgtg ttaggcctgc
      181 cgccagcgtt caatctgagc catgatcaaa ctcttcaatt taaaagtttg atgctcaaag
      241 aattaaactt cgtaatgaat tacgtgttca ctcttgagac ttggtattca tttgtcgtct
      301 tgcgacgtta agaatccgta tcttcgagtg cccacacaga ttgcctgata aattgttaaa
      361 gagcagtgcc gcttcgcttt ttctcagcgg cgctgaggtg tgcataatac gctttcccgc
      421 tacagagtca agcatttatt tttgctttct ctgccggaat tctcaggaga accccgctga
      481 cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc
      541 ggcctgacaa gcgaaaaata taaaaacttt atcgttcgct cacttttcag gcaaaacatc
      601 ttaaatatag tcttttccgt ctaacttata gacaaaaacg agccccgaag g
//
LOCUS       DADMFL010000337          633 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.345, whole genome shotgun sequence.
ACCESSION   DADMFL010000337 DADMFL010000000
VERSION     DADMFL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.345"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>633
                     /locus_tag="JNZ27_004381"
     CDS             <1..>633
                     /locus_tag="JNZ27_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HAY8666409.1"
                     /translation="RSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
                     SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          149 a          159 c          189 g          136 t
ORIGIN      
        1 ccggtcaaaa cagcgttacg gtgccccacg cctgacggat gaactgcgtg ctcagggtta
       61 cccctttaac gtaaaaaccg tggcggcaag cctgcgccgt cagggactga gggcaaaggc
      121 ctcccggaag ttcagcccgg tcagctaccg cgcacacggc ctgcctgtgt cagaaaatct
      181 gttggagcag gatttttacg ccagtggccc gaaccagaag tggccaggag acatcacgta
      241 cttacgtaca gatgaaggct ggctgtatct ggcagtggtc attgacctgt ggtcacgtgc
      301 cgttattggc tggtcaatgt cgccacgcat gacggcgcaa ctggcctgcg atgccctgca
      361 gatggcgctg tggcggcgta agaggccccg gaacgttatc gttcacacgg accgtggagg
      421 ccagtactgt tcagcagatt atcaggcgca actgaaacgg cataatctgc gtggaagtat
      481 gagcgcaaaa ggttgctgct acgataatgc ctgcgtggaa agcttctttc attcgctgaa
      541 agtggaatgt atccatggag aacactttat cagccgggaa ataatgcggg caacggtgtt
      601 taattatatc gaatgtgatt acaatcggtg gcg
//
LOCUS       DADMFL010000338          632 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.346, whole genome shotgun sequence.
ACCESSION   DADMFL010000338 DADMFL010000000
VERSION     DADMFL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.346"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>632
                     /locus_tag="JNZ27_004382"
     CDS             <1..>632
                     /locus_tag="JNZ27_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666410.1"
                     /translation="WLNWLEKQPEGAVRPVVIESVTKIMACGTTLMGYTQWCCSSPDC
                     CHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPCQYWSLVFHNR
                     WLLAEMSRIAADVILEICHQADVEPGIFTVSHTWGRDQQWHPHIHLSTTAGGVTSGHT
                     WKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFL"
BASE COUNT          145 a          160 c          192 g          135 t
ORIGIN      
        1 tggcttaact ggctggagaa acaaccggaa ggggcagtcc gtccggtggt aattgaatca
       61 gtgacaaaaa tcatggcctg cgggaccaca ctgatgggct acacgcagtg gtgttgttca
      121 tcaccggact gttgccatac aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg
      181 cactgcgggg tgaaggctgg cgcacagtgg atacagtatc tgctgagcct ggtccccgac
      241 tgcccgtggc agcatattgt gttcacactt ccctgccagt actggtccct ggtgttccac
      301 aaccggtggt tactggcaga gatgagccgc attgcagcgg atgtgatact ggaaatctgc
      361 catcaggcag atgtggagcc ggggatattc acggtgagcc acacatgggg gcgtgaccag
      421 cagtggcatc cgcatatcca tttatcgaca actgccggtg gtgtgacgtc gggccacacc
      481 tggaaaaatc ttcattttta cgcccgtaag gtgatgagca tgtggcgcta ccggataaca
      541 cggttgctgt cacggaaata ccctgacctg gtgatgccgg atgcgctggc agcagaagga
      601 agcagtaaac gggaatggaa tcgcttcctg ga
//
LOCUS       DADMFL010000339          630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.347, whole genome shotgun sequence.
ACCESSION   DADMFL010000339 DADMFL010000000
VERSION     DADMFL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.347"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>630
                     /gene="aadA"
                     /locus_tag="JNZ27_004383"
     CDS             <1..>630
                     /gene="aadA"
                     /locus_tag="JNZ27_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HAY8666411.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQY"
BASE COUNT          147 a          160 c          178 g          145 t
ORIGIN      
        1 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
       61 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      121 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      181 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      241 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      301 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      361 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      421 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      481 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      541 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      601 ggcaatggag cgcctgccgg cccagtatca
//
LOCUS       DADMFL010000340          623 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.348, whole genome shotgun sequence.
ACCESSION   DADMFL010000340 DADMFL010000000
VERSION     DADMFL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.348"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>623
                     /locus_tag="JNZ27_004384"
     CDS             <1..>623
                     /locus_tag="JNZ27_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8666412.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDN
                     GSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          148 a          152 c          196 g          127 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacgtca tgctgggagc
      421 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      481 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      541 gaagagcacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
      601 gataaagcgt gactacatca gtg
//
LOCUS       DADMFL010000341          615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.349, whole genome shotgun sequence.
ACCESSION   DADMFL010000341 DADMFL010000000
VERSION     DADMFL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.349"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNZ27_004385"
     CDS             complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="JNZ27_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY8666413.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSDFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          186 a          123 c           91 g          215 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa atcactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgtt
//
LOCUS       DADMFL010000342          606 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.350, whole genome shotgun sequence.
ACCESSION   DADMFL010000342 DADMFL010000000
VERSION     DADMFL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.350"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            174..>606
                     /locus_tag="JNZ27_004386"
     CDS             174..>606
                     /locus_tag="JNZ27_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8666414.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAG"
BASE COUNT          153 a          130 c          144 g          179 t
ORIGIN      
        1 attgctgtta gtgaagctga ctttattatt catcattcag ttcctcatgg agttgtcggt
       61 tcgttttgac ggttggtgat atcactatag atattgatca ttaagttgat tagaccctaa
      121 atcatgatta gacctatcat ttaaatgatt aatagattgt atggagatac tgaatgaagc
      181 gtgaagaaat cgctgatctg atggcgtttg tcgtcgttgc agaggagcgt agcttcactc
      241 gcgcagcagc ccgcctgagc atggtgcagt cagctttaag ccagatagtg cgccgtatag
      301 aagaacgatt gggattgcgg cttctgacgc gaaccacgcg cagcgttgta cccactgaag
      361 cgggcgagca tcttttgtct gttcttggcc ctatattgca tgacatagat tctgccctga
      421 catccctgag cgatctgcag aaccgcccat ccgggacaat acgtattact actgtagaac
      481 atgcagcaaa aacgatattg ttaccagcca tgcgcacatt cctgaaatcg catcctgaaa
      541 ttgatattca gcttaccatt gattatggtt tgaccgatgt cgtttctgaa cgttttgatg
      601 caggcg
//
LOCUS       DADMFL010000343          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.351, whole genome shotgun sequence.
ACCESSION   DADMFL010000343 DADMFL010000000
VERSION     DADMFL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.351"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..297
                     /locus_tag="JNZ27_004387"
     CDS             <1..297
                     /locus_tag="JNZ27_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY8666415.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JNZ27_004388"
     CDS             complement(405..>566)
                     /locus_tag="JNZ27_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666416.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADMFL010000344          564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.352, whole genome shotgun sequence.
ACCESSION   DADMFL010000344 DADMFL010000000
VERSION     DADMFL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.352"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
BASE COUNT          150 a          156 c          125 g          133 t
ORIGIN      
        1 ttccctcgcg atattaacgt aatatcttaa ttgtatgcac cgccagaatg cgttcagtgc
       61 gatcacggca gcagacaggt aaaaatagca acaaaccacc cgaaaaaccg ccgcgatcgc
      121 gtatgataaa ttttaaccac atgcatacct gtgcagcaag gcgaatcacg cacggatttc
      181 cagccagaga cacctgaaac gtaaccggct catttaaacc gtctggtctg ttacctccgg
      241 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
      301 gaatcactga agctttacgc cttcatttcg aagaacattt accacgggtt gtggccggac
      361 gtcgcctggg cgtaccaaaa tcaactgtct gtggtatgtt cgtgcgcttc cgcaacgctg
      421 gcctttcatg gcctctgccc gcaggtatgt cggagcagga acttgatgcc cttctttacg
      481 gaagtgcctc cacagtacct gtcgtactta ctgaaagcac cgttatgccc aaactcccgg
      541 tggtaaaaaa aacgtccccg gcgg
//
LOCUS       DADMFL010000345          556 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.353, whole genome shotgun sequence.
ACCESSION   DADMFL010000345 DADMFL010000000
VERSION     DADMFL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.353"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>556
                     /locus_tag="JNZ27_004389"
     CDS             <1..>556
                     /locus_tag="JNZ27_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY8666417.1"
                     /translation="VNMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNEAFNRELAKLN
                     QSLRETLSKFNKTTSKNNYSENASNSLDVLATSAIKAGTAIYGFQTALEAYRKVMEIG
                     LKKEASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETFSSFAKFSAGAGDMNADQ
                     KESLFSNVIGTSRLMGLSTDEING"
BASE COUNT          186 a           88 c          121 g          161 t
ORIGIN      
        1 gcgtgaatat gagcaagctt cgtgacttta atcctatgct tgagaagtat tttaaaagcc
       61 agtattacag cttaagcagt aaatccaaga cacttggaaa cgaagctttt aatcgtgagt
      121 tagcaaaatt aaatcaatct cttcgtgaaa cactttctaa gtttaataag accaccagca
      181 aaaataatta cagtgaaaat gcttcaaata gtctggatgt attagctaca agtgcaatca
      241 aagcaggtac agccatttat ggttttcaga cagccttaga agcttatagg aaggttatgg
      301 agattggtct gaaaaaagaa gcatcacaac gagcggcaca attcgtttta ggtgatgaag
      361 gtgctaaacg agctacagaa tttgttaaga atcttgctga tagtagtggt gtagaccaga
      421 ttgaaacatt tagcagtttt gctaagtttt ccgctggtgc tggtgatatg aatgcagacc
      481 agaaagaatc cttattttct aatgtgattg gtacatcccg actaatgggt ttaagcactg
      541 atgagattaa cgggat
//
LOCUS       DADMFL010000346          553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.354, whole genome shotgun sequence.
ACCESSION   DADMFL010000346 DADMFL010000000
VERSION     DADMFL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.354"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(28..>553)
                     /locus_tag="JNZ27_004390"
     CDS             complement(28..>553)
                     /locus_tag="JNZ27_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8666418.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          145 a          138 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagaccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aag
//
LOCUS       DADMFL010000347          552 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.355, whole genome shotgun sequence.
ACCESSION   DADMFL010000347 DADMFL010000000
VERSION     DADMFL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.355"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>552
                     /locus_tag="JNZ27_004391"
     rRNA            <1..>552
                     /locus_tag="JNZ27_004391"
                     /product="16S ribosomal RNA"
BASE COUNT          139 a          136 c          164 g          113 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tg
//
LOCUS       DADMFL010000348          546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.356, whole genome shotgun sequence.
ACCESSION   DADMFL010000348 DADMFL010000000
VERSION     DADMFL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.356"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(91..240)
                     /gene="hokB"
                     /locus_tag="JNZ27_004392"
     CDS             complement(91..240)
                     /gene="hokB"
                     /locus_tag="JNZ27_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY8666419.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(340..480)
                     /locus_tag="JNZ27_004393"
                     /pseudo
     CDS             complement(340..480)
                     /locus_tag="JNZ27_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          144 a          127 c          126 g          149 t
ORIGIN      
        1 aaaaacatgc acgaactgaa gtgtaaaagg ggtgccatga gaaaatgaat ctgctagtca
       61 gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca tgagcgcagc
      121 aacctcctta tcaccgtccc ggaaccgcag ttcgtagagc gtttgtcggg tcaggagtgt
      181 gaatgccaga atcgtaatgc agataatgag cagacacacc accagagggt tgcgcttcat
      241 agcctttctc cttgccggat ggcgggtaag aggctaacat ctgaattgct tggttcattc
      301 gttggcctcg gtttgtactg accccaaaaa gttggacagt taaacacgag gcatataggt
      361 ctgattccga tattcatttg gagtcagacc ttttaatttc aggctaattc ttctgctgtt
      421 gtagtattca atatattccg taacagcatc cttcagttcg cttatattac tgaactcatt
      481 agactggccc cctgaatctc cagacaacca gtatcactta tttaagtgat agtcttaata
      541 ctagtt
//
LOCUS       DADMFL010000349          533 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.357, whole genome shotgun sequence.
ACCESSION   DADMFL010000349 DADMFL010000000
VERSION     DADMFL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.357"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            5..>533
                     /gene="fimC"
                     /locus_tag="JNZ27_004394"
     CDS             5..>533
                     /gene="fimC"
                     /locus_tag="JNZ27_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY8666420.1"
                     /translation="MTFCLLAGILMFMAMMVAGRAEAGVALGATRVIYPAGQKQVQLA
                     VTNNDENSTYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDR
                     ESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSA
                     NSLTLINPTPYYLTVT"
BASE COUNT          162 a          113 c          126 g          132 t
ORIGIN      
        1 ggaaataaca ttctgcttgc tggcaggtat cctgatgttc atggcaatga tggttgccgg
       61 acgcgctgaa gcaggagtgg ccttaggtgc gactcgcgta atttatccgg cagggcaaaa
      121 acaagtgcaa cttgccgtga caaataatga tgaaaatagt acctatttaa ttcaatcatg
      181 ggaggaaaat gccgatggtg taaaggatgg tcgttttatc gtgacgcctc ctctgtttgc
      241 gatgaaggga aaaaaagaga ataccttacg tattcttgat gcaacaaata accaattgcc
      301 acaggaccgg gaaagtttat tctggatgaa cgttaaagcg attccgtcaa tggataaatc
      361 aaaattgact gagaatacgc tacagctcgc aattatcagc cgcattaaac tgtactatcg
      421 cccggctaaa ttagcgttgc cacccgatca ggccgcagaa aaattaagat ttcgtcgtag
      481 cgcgaattct ctgacgctga ttaacccgac accctattac ctgacggtaa cag
//
LOCUS       DADMFL010000350          525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.358, whole genome shotgun sequence.
ACCESSION   DADMFL010000350 DADMFL010000000
VERSION     DADMFL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.358"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>525)
                     /locus_tag="JNZ27_004395"
     CDS             complement(<1..>525)
                     /locus_tag="JNZ27_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8666421.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNADNAMQ"
BASE COUNT          149 a          124 c          105 g          147 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaat
//
LOCUS       DADMFL010000351          522 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.359, whole genome shotgun sequence.
ACCESSION   DADMFL010000351 DADMFL010000000
VERSION     DADMFL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.359"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>522)
                     /locus_tag="JNZ27_004396"
     CDS             complement(<1..>522)
                     /locus_tag="JNZ27_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8666422.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPA"
BASE COUNT          157 a          148 c          109 g          108 t
ORIGIN      
        1 gcaggcgcgg ttaccgccat cgccacatac aggagcgaca taacaaacat cgcaatccct
       61 gccacagaac cgacgatttt tagcgatttc ataccgcgtg aagcaaccca cataaagaag
      121 ataaacagca ccagcgttaa cccctgtaac gctacgactg aatattcttt gattagcgaa
      181 ccgtcgcctt tcatcgccca accgagcgca atcagaattg cctggggttt ttgtgccaga
      241 tagggaatat gcaccaccca gtaggtccac gcggcgagat aagccagtcc gggtcccatc
      301 gtgtgtttaa tccaggtact gaccccgccc ttcccatctt tgaaggtcga gcctaactga
      361 ccaacaatta gcgcataagg tgtgaaatag agtgcaaaga taaacaccca tgaaaaaaca
      421 accaccagcc cctggttggc atagttatta acaacgttgc caaaacccca gacagtgata
      481 aaagacatca gcgcaatgtt gtaccatcgc aactgttttt cc
//
LOCUS       DADMFL010000352          521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.360, whole genome shotgun sequence.
ACCESSION   DADMFL010000352 DADMFL010000000
VERSION     DADMFL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.360"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(208..>521)
                     /locus_tag="JNZ27_004397"
     CDS             complement(208..>521)
                     /locus_tag="JNZ27_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8666423.1"
                     /translation="EQEAKPFTFEWNGRIWNAGPNSLGRLSPVVMLAKSVAAQTNMTW
                     SDADNQQVQLTTQELEELATAMIQAIVERNDEIYRRQREMKEELSGLDDLASIRAFDV
                     E"
BASE COUNT          113 a          172 c          116 g          120 t
ORIGIN      
        1 aaacggctca caactgcacc attcataatc acatcgcata aaccacagac cggactgact
       61 ccggaaaaac ggaggcccgc ccccgggcct cccctgattc atccgtttcc ctgttcagcc
      121 tgacagcacg ccccggcggc cggatgacag actccgcttc ggtaagcaaa gcggtcttct
      181 gtgattccgc cagttgcggc ttattcatta ctcaacgtca aacgcccgaa ttgaagccaa
      241 atcatccaga ccgctcagct cctctttcat ttcacgctga cggcgataaa tctcatcgtt
      301 gcgctcaaca atggcctgta tcatcgctgt ggccagttcc tcaagttcct gcgtcgtcag
      361 ctgcacctgc tgattatccg catcactcca cgtcatattt gtttgtgccg cgacagattt
      421 tgccagcatg accaccgggg acaggcggcc cagtgagttg gggccagcat tccagatacg
      481 accgttccat tcaaacgtga acggcttcgc ctcctgttct g
//
LOCUS       DADMFL010000353          521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.361, whole genome shotgun sequence.
ACCESSION   DADMFL010000353 DADMFL010000000
VERSION     DADMFL010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.361"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            452..>521
                     /locus_tag="JNZ27_004398"
     CDS             452..>521
                     /locus_tag="JNZ27_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8666424.1"
                     /translation="MEPVYVILNALLDSGRFTRKLIL"
BASE COUNT          119 a          128 c          143 g          131 t
ORIGIN      
        1 acccgattag ttgttaatca tatttaatca tagtgattac tttcagacgg aacaaggcaa
       61 ataatgcgag tcagcgcata taaggcggca tacggtggtt acatgtagca caggtgtaaa
      121 accgccgaag cccggcgcaa gccggtacgg tcgtccgaat atcaccagtc cccctccggc
      181 tctgcgaggc catgcggcct gtcagcgatg aaaatgcctc tgtgcgcggt cggagtggtc
      241 ccgacaaggg tttacccgca gtcggggcgt atctccgcgt tagcgggccg tgagggccgc
      301 ttacgagcgt gtatgagaga cgtacagtag ttttgtccgc tcacaccagt acaaaccagt
      361 cgaaataccg gtaacgacga ttactcttgt ctctgatgcc ggtggactac gagattttat
      421 ttactggcgt cctgatgcgg tcaggactgg tatggaacct gtttatgtga tacttaatgc
      481 tcttctggat tccgggcgat ttacgcgtaa acttattctg t
//
LOCUS       DADMFL010000354          513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.362, whole genome shotgun sequence.
ACCESSION   DADMFL010000354 DADMFL010000000
VERSION     DADMFL010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.362"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..502
                     /locus_tag="JNZ27_004399"
     CDS             <1..502
                     /locus_tag="JNZ27_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8666425.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DADMFL010000355          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.363, whole genome shotgun sequence.
ACCESSION   DADMFL010000355 DADMFL010000000
VERSION     DADMFL010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.363"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>512)
                     /locus_tag="JNZ27_004400"
     CDS             complement(<1..>512)
                     /locus_tag="JNZ27_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8666426.1"
                     /translation="REGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVL
                     QRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENML
                     DQAFKKLNPHEHPVLQSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFG
                     TLKSECFYLD"
BASE COUNT          133 a          121 c          101 g          157 t
ORIGIN      
        1 atcaagataa aaacactccg actttaaggt tccaaagaaa cactccacca cagcattatc
       61 cagacaattg ccttttctgg acatgctttg tttaatacca tgttctttaa ggatattttg
      121 atatcttctc atacgatact gccatccctg gtcagactgc agaacaggat gctcgtgagg
      181 attaagcttt ttgaatgcct gatcgagcat attctcaacc atgttcatca ctggtctttc
      241 cgaaaggctg taagaaataa cttcgttgtt gaagagatct attactggag acaaatacag
      301 cttgcgccca ttgactgcaa attcagtaac atcggtaacc cacttctcgt ttggccgcgt
      361 agccttgaaa tctctttgga gaacattagg ggcggtttgc cctacctctc ctctgtaaga
      421 gcggtatcgc ttgaccttaa tcgctgcttt aagtgagagg gttcccatca ggcgctgaac
      481 agctttatgg ttaatctgtt tcccttctcg at
//
LOCUS       DADMFL010000356          511 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.364, whole genome shotgun sequence.
ACCESSION   DADMFL010000356 DADMFL010000000
VERSION     DADMFL010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.364"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..511
                     /locus_tag="JNZ27_004401"
                     /pseudo
     CDS             1..511
                     /locus_tag="JNZ27_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          126 a          128 c          142 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccggtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DADMFL010000357          496 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.365, whole genome shotgun sequence.
ACCESSION   DADMFL010000357 DADMFL010000000
VERSION     DADMFL010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.365"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>496)
                     /locus_tag="JNZ27_004402"
     CDS             complement(<1..>496)
                     /locus_tag="JNZ27_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAY8666427.1"
                     /translation="EVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPYVN
                     QTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKLGE
                     YYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGTPV
                     NGYFL"
BASE COUNT          143 a          119 c          109 g          125 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttc
//
LOCUS       DADMFL010000358          495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.366, whole genome shotgun sequence.
ACCESSION   DADMFL010000358 DADMFL010000000
VERSION     DADMFL010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.366"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>495)
                     /locus_tag="JNZ27_004403"
     rRNA            complement(<1..>495)
                     /locus_tag="JNZ27_004403"
                     /product="16S ribosomal RNA"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DADMFL010000359          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.367, whole genome shotgun sequence.
ACCESSION   DADMFL010000359 DADMFL010000000
VERSION     DADMFL010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.367"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..410)
                     /locus_tag="JNZ27_004404"
     CDS             complement(<1..410)
                     /locus_tag="JNZ27_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY8666428.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          110 a          117 c           94 g          152 t
ORIGIN      
        1 tcatgttcga acaccactcc aacatctgtt tgaacacctg ttaaatttct ctcttgtttt
       61 gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt tcctgcggct
      121 gattttttgg ttaatttttc tctgacagat gccagatctt cctcaatccg aagatgcatc
      181 cattcgtcgc cgttatcaca aaaaaactcc cgcaaggatg gttcaacatc aacccatcgc
      241 tcgttagtca gacgggcaat ttttgccaac ctgtttttgg gtattggctt tcctgtttgc
      301 cagtaattga acatcagcaa caaatacgcg ccgtgctcct ctgctgacaa atgcatggtg
      361 tcagccagat aatcagctat gtacagttgc atgtatggta atgcggccat aattgccccg
      421 tatgatgcta cccggttgct tagaataagt gaggtagcct gagtttaacg gac
//
LOCUS       DADMFL010000360          464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.368, whole genome shotgun sequence.
ACCESSION   DADMFL010000360 DADMFL010000000
VERSION     DADMFL010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.368"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>464
                     /locus_tag="JNZ27_004405"
     CDS             <1..>464
                     /locus_tag="JNZ27_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8666429.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DADMFL010000361          462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.369, whole genome shotgun sequence.
ACCESSION   DADMFL010000361 DADMFL010000000
VERSION     DADMFL010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.369"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..183
                     /locus_tag="JNZ27_004406"
     CDS             <1..183
                     /locus_tag="JNZ27_004406"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666430.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..>462
                     /locus_tag="JNZ27_004407"
     CDS             278..>462
                     /locus_tag="JNZ27_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY8666431.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          137 a           91 c          136 g           98 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aa
//
LOCUS       DADMFL010000362          450 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.370, whole genome shotgun sequence.
ACCESSION   DADMFL010000362 DADMFL010000000
VERSION     DADMFL010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.370"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..314
                     /locus_tag="JNZ27_004408"
     CDS             <1..314
                     /locus_tag="JNZ27_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8666432.1"
                     /translation="EQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAW
                     SDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDV
                     K"
BASE COUNT          109 a          105 c          151 g           85 t
ORIGIN      
        1 cagaacagga ggcgaagccg ttcacgtttg aatggaacgg tcgtatctgg aatgctggtc
       61 ccgactcact gggccgcctg tccccggtag tcatgctggc aaaatctgtc acagcacaaa
      121 cacatatggc gtggagcgat gcggataatc agcaggtgaa actgtcgatg ccggaactgg
      181 aagaactggc ggcagcaatg gtgcaggcgc aggtcgatcg caacgatgag atttatcgcc
      241 gtcagcgtga aatgaaagag gagctgagcg gtctggatga tttggcttca attcgggcgt
      301 ttgacgttaa gtaatgaata agccgcaact ggcggaatca cagaagaccg ctttgcttac
      361 cgaggcggag tctgtcatcc ggccgccggg gtgtgcggtc aggctgaaca gagaaacgga
      421 tgaatccggg gaggcccggg ggcgggcctc
//
LOCUS       DADMFL010000363          441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.371, whole genome shotgun sequence.
ACCESSION   DADMFL010000363 DADMFL010000000
VERSION     DADMFL010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.371"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            211..>441
                     /locus_tag="JNZ27_004409"
     CDS             211..>441
                     /locus_tag="JNZ27_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8666433.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          105 c           62 g          135 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccttggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg a
//
LOCUS       DADMFL010000364          435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.372, whole genome shotgun sequence.
ACCESSION   DADMFL010000364 DADMFL010000000
VERSION     DADMFL010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.372"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
BASE COUNT          124 a           79 c           77 g          155 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaaaaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggag
//
LOCUS       DADMFL010000365          427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.373, whole genome shotgun sequence.
ACCESSION   DADMFL010000365 DADMFL010000000
VERSION     DADMFL010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.373"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>427
                     /locus_tag="JNZ27_004410"
     CDS             <1..>427
                     /locus_tag="JNZ27_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8666434.1"
                     /translation="YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVG
                     WAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMS
                     RRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          101 a          104 c          138 g           84 t
ORIGIN      
        1 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
       61 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      121 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggctcacc atgaaagcgc
      181 tggaaatggc atgggaaacc cgtggtaagc ccggcggggt gatgttccac agcgatcagg
      241 gcagtcatta tacgagcagg cagttccggc agttattgtg gcgataccag atcagacaga
      301 gtatgagccg gcgcggaaac tgctgggata acagcccaat ggaacgcttc ttcaggagtc
      361 tgaagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
      421 taacgga
//
LOCUS       DADMFL010000366          427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.374, whole genome shotgun sequence.
ACCESSION   DADMFL010000366 DADMFL010000000
VERSION     DADMFL010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.374"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(9..299)
                     /locus_tag="JNZ27_004411"
     CDS             complement(9..299)
                     /locus_tag="JNZ27_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY8666435.1"
                     /translation="MNAHWSSKKSNFLRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(268..>427)
                     /locus_tag="JNZ27_004412"
     CDS             complement(268..>427)
                     /locus_tag="JNZ27_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8666436.1"
                     /translation="YIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRE
                     RSLEFEKE"
BASE COUNT          130 a          108 c           78 g          111 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatatag
//
LOCUS       DADMFL010000367          418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.375, whole genome shotgun sequence.
ACCESSION   DADMFL010000367 DADMFL010000000
VERSION     DADMFL010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.375"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>418
                     /locus_tag="JNZ27_004413"
     CDS             <1..>418
                     /locus_tag="JNZ27_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666437.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAG"
BASE COUNT          105 a           99 c          125 g           89 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggtt
//
LOCUS       DADMFL010000368          399 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.376, whole genome shotgun sequence.
ACCESSION   DADMFL010000368 DADMFL010000000
VERSION     DADMFL010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.376"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>399
                     /locus_tag="JNZ27_004414"
     CDS             <1..>399
                     /locus_tag="JNZ27_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8666438.1"
                     /translation="ERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFG
                     NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
                     YISIMPKPDGLTAAKNLAEAFEHYNEWHPH"
BASE COUNT          103 a           96 c          125 g           75 t
ORIGIN      
        1 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
       61 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      121 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      181 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      241 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      301 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      361 cagaggcgtt cgagcattat aacgaatggc atccgcata
//
LOCUS       DADMFL010000369          397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.377, whole genome shotgun sequence.
ACCESSION   DADMFL010000369 DADMFL010000000
VERSION     DADMFL010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.377"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            281..388
                     /gene="ldrD"
                     /locus_tag="JNZ27_004415"
     CDS             281..388
                     /gene="ldrD"
                     /locus_tag="JNZ27_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAY8666439.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          108 a           90 c          116 g           83 t
ORIGIN      
        1 aacggttgca agcggataat attctgcgga atgcgcagca cagagtgaag atgagacgga
       61 ttccggaaca gaaaaacagt cgtagaatca gcgtcaagcc ggaaggattc cggtgaggcg
      121 caatgttgcg ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca
      181 cgttgcaggt atgcacctga caacaccacg ggggccattc ttgcatccaa acaatacaag
      241 aatagccgcg aaacgttgtc attacaatac aggcggctat atgacgttcg cagaactggg
      301 catggccttc tggcatgatt tagcggctcc ggtcattgct ggtattcttg ccagtatgat
      361 cgtgaactgg ctgaacaagc ggaagtaacg tgccatg
//
LOCUS       DADMFL010000370          396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.378, whole genome shotgun sequence.
ACCESSION   DADMFL010000370 DADMFL010000000
VERSION     DADMFL010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.378"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>396
                     /locus_tag="JNZ27_004416"
     CDS             <1..>396
                     /locus_tag="JNZ27_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666440.1"
                     /translation="RWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLV
                     RYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVK
                     LPTRRGRAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          120 a           76 c          123 g           77 t
ORIGIN      
        1 atcgctggga aatcgaactg ggatacaggg agataaaaca gacgatgcaa ctgagcaggc
       61 tgacgctgag aagtaaaaag ccggagcttg tggagcaaga gctgtggggt gtcttactgg
      121 cttataatct ggtgagatat cagatgatta aaatggcgga acatctgaaa ggttactggc
      181 cgaatcaact gagtttctca gaatcatgcg gaatggtgat gagaatgctg atgacattgc
      241 agggcgcttc accgggacgt ataccggagc tgatgcgcga tcttgcaagt atgggacaac
      301 ttgtgaaatt accgacgaga aggggaaggg ccttcccgag agtggtaaag gagaggccct
      361 ggaaataccc cacagccccg aaaaagagcc agtcag
//
LOCUS       DADMFL010000371          392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.379, whole genome shotgun sequence.
ACCESSION   DADMFL010000371 DADMFL010000000
VERSION     DADMFL010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.379"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>392)
                     /locus_tag="JNZ27_004417"
     CDS             complement(<1..>392)
                     /locus_tag="JNZ27_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HAY8666441.1"
                     /translation="LVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAE"
BASE COUNT          124 a           91 c           83 g           94 t
ORIGIN      
        1 tgctcggcgt tcattccgga catctgcttc tcaaggcttt ccccctcgga gaaaaccaga
       61 tatttgccca ataaaatacc agacgcagca ccaatcggat aaaaggtctg gctgatattg
      121 agccgcaatg tggcataggc ttttggaccg atcattgaac tgtatgtgtt cgctgcagtt
      181 tcaaggaaac tcaggccaat cgcaatcgca aaaatagctg caaggaacat agtgtaggtt
      241 gccatatgcg aggcagggaa aaaaagtgta caaccaccaa tatacagcgt caggccaatt
      301 aaaattgcca ccttataact ggtcttttta atcacaaggg atgctggtat tgcaattaaa
      361 aaataacctc cataaaatgc gctctgcacc aa
//
LOCUS       DADMFL010000372          390 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.380, whole genome shotgun sequence.
ACCESSION   DADMFL010000372 DADMFL010000000
VERSION     DADMFL010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.380"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(101..>390)
                     /locus_tag="JNZ27_004418"
     CDS             complement(101..>390)
                     /locus_tag="JNZ27_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8666442.1"
                     /translation="EDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTLVTRLSEM
                     NVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD"
BASE COUNT           91 a           94 c           86 g          119 t
ORIGIN      
        1 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcatac
       61 cttcgtgaag ctgttgaagg gtggattctt aacggtggcg ttaatcagaa atgctcctga
      121 tgtgccagaa caatttatca gcgtgaagct catacgcttt gcgctgagcc tctatccagt
      181 cgtgtttatt gtatacggac attatcccgc caagttcatg accgagcatt cgctcagtaa
      241 catgaggctc gacattcatc tcggacagcc gtgtaacaag cgttcttctg aaatcatgtg
      301 ttcgccagta acctagatcc atccctcccc ttattctatt tacgaatcgg ttggctgcag
      361 cgatgctgat cggtttgttt atgtcttctc
//
LOCUS       DADMFL010000373          385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.381, whole genome shotgun sequence.
ACCESSION   DADMFL010000373 DADMFL010000000
VERSION     DADMFL010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.381"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>385
                     /locus_tag="JNZ27_004419"
     CDS             <1..>385
                     /locus_tag="JNZ27_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666443.1"
                     /translation="DLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFE
                     RKDDSSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQ
                     ELNNSQVSCKGSVDSTITDLLEKAL"
BASE COUNT          142 a           54 c           79 g          110 t
ORIGIN      
        1 cggatttatc taaggtgaag atagaaatta cacttgcctc agcttctact aaagggattc
       61 gtgaaggaca tacagtcatt atatttaaac aatctgatgg ttcgactaat cgttatgaag
      121 ggaagtcttt tgaaagaaaa gatgatagtt cattacacct gattactaac aaggttctgg
      181 cgtgttacca acgcgaagct aacaagaaaa tagcgcggct attaaataat catcagaagt
      241 taaataatat acaggagtta aatgattcgc aggagttaaa taattcacag aagttaaata
      301 attcgcagga gttaaataac tcgcaggtga gttgtaaagg ttcagttgat tctacgatta
      361 cggatttatt agaaaaagca ttgaa
//
LOCUS       DADMFL010000374          377 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.382, whole genome shotgun sequence.
ACCESSION   DADMFL010000374 DADMFL010000000
VERSION     DADMFL010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.382"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
BASE COUNT          112 a           95 c           69 g          101 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacaca
//
LOCUS       DADMFL010000375          374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.383, whole genome shotgun sequence.
ACCESSION   DADMFL010000375 DADMFL010000000
VERSION     DADMFL010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.383"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>374
                     /locus_tag="JNZ27_004420"
     CDS             <1..>374
                     /locus_tag="JNZ27_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8666444.1"
                     /translation="LRKAVAVAIGGGAIAIASVLITGPGGNDGLEGVSYIPYKDIVGV
                     WTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFV
                     YNVGAGNFRTSMLLRKINQGDI"
BASE COUNT          105 a           92 c           91 g           86 t
ORIGIN      
        1 attacgcaag gctgttgcag ttgctattgg tggtggggct attgccatag cgtctgtgct
       61 catcactggc ccaggtggta acgatggtct ggaaggtgtc agctacatac catacaaaga
      121 tatcgttggc gtatggactg tatgccacgg acacaccgga aaagacatca tgctcggtaa
      181 aacgtatacc gaagcagaat gcaaagccct cctgaataaa gaccttgcca ctgtcgccag
      241 acaaattaac ccgtacatca aagtcgatat accggaaaca acgcgcggcg ctctttactc
      301 gttcgtttac aacgtgggcg ctggcaattt cagaacatcg atgcttcttc gcaaaataaa
      361 ccagggcgat atca
//
LOCUS       DADMFL010000376          368 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.384, whole genome shotgun sequence.
ACCESSION   DADMFL010000376 DADMFL010000000
VERSION     DADMFL010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.384"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..222
                     /locus_tag="JNZ27_004421"
     CDS             <1..222
                     /locus_tag="JNZ27_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY8666445.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..>368
                     /locus_tag="JNZ27_004422"
     CDS             268..>368
                     /locus_tag="JNZ27_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8666446.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLS"
BASE COUNT          113 a           79 c           88 g           88 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaa
//
LOCUS       DADMFL010000377          363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.385, whole genome shotgun sequence.
ACCESSION   DADMFL010000377 DADMFL010000000
VERSION     DADMFL010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.385"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..>363)
                     /locus_tag="JNZ27_004423"
     CDS             complement(<1..>363)
                     /locus_tag="JNZ27_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8666447.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAM
                     QITWRGMYQRLLKVDPLKC"
BASE COUNT           83 a          115 c           68 g           97 t
ORIGIN      
        1 gcacttcagc gggtcaacct tcagtaacct ctgatacatc cctctccagg tgatttgcat
       61 cgccgttttt ctcactgtct ccgttatgat gtacaccact tcttccagta accgccgttt
      121 cgccggactc aaaaaaccgt agtacctcac catacggaac cccttatccg ccacatgcca
      181 ggagaacctt tccatgaact catctccact catcaacagg tattcttccc gttttgttcg
      241 gtgactgttg taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat
      301 cggcaccggt ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc
      361 cat
//
LOCUS       DADMFL010000378          356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.386, whole genome shotgun sequence.
ACCESSION   DADMFL010000378 DADMFL010000000
VERSION     DADMFL010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.386"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            65..140
                     /locus_tag="JNZ27_004424"
     tRNA            65..140
                     /locus_tag="JNZ27_004424"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Glu,seq:ttc)
BASE COUNT           97 a           77 c           88 g           94 t
ORIGIN      
        1 agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg aagcagactg
       61 atacgtcccc ttcgtctaga ggcctaggac accgcccttt cacggcggta acaggggttc
      121 gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcacctgc cttaatatct
      181 caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg gatcaagctg
      241 aaaattgaaa cactgaacaa tgaaagttgt tcgtgagtct ctcaaatttt cgcaactctg
      301 aagtgaaaca tcttcgggtt gtgaggttaa gcgactaagc gtacacggtg gatgcc
//
LOCUS       DADMFL010000379          353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.387, whole genome shotgun sequence.
ACCESSION   DADMFL010000379 DADMFL010000000
VERSION     DADMFL010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.387"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            36..>353
                     /locus_tag="JNZ27_004425"
     CDS             36..>353
                     /locus_tag="JNZ27_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666448.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSV"
BASE COUNT           90 a           88 c           88 g           87 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gtt
//
LOCUS       DADMFL010000380          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.388, whole genome shotgun sequence.
ACCESSION   DADMFL010000380 DADMFL010000000
VERSION     DADMFL010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.388"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..208)
                     /locus_tag="JNZ27_004426"
     CDS             complement(<1..208)
                     /locus_tag="JNZ27_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8666449.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JNZ27_004427"
     CDS             complement(205..>351)
                     /locus_tag="JNZ27_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY8666450.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADMFL010000381          346 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.389, whole genome shotgun sequence.
ACCESSION   DADMFL010000381 DADMFL010000000
VERSION     DADMFL010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.389"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..346
                     /locus_tag="JNZ27_004428"
                     /pseudo
     CDS             1..346
                     /locus_tag="JNZ27_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT           81 a           77 c           78 g          110 t
ORIGIN      
        1 atgttagaga acacccttga accgtataag tatatgatta tgattctggt tgtggtcatg
       61 gtgttgttcc ttttaacccg cttcccaacc tgtaaagtgg cacagacaag tcaccataaa
      121 cgcccgtcag caatggatac gctgcgctat ctggctaaaa attcgcgctt tcgtcgtggt
      181 attgtggctc aattccttta tgtgggtatg taggtcgcag tatggtcatt taccatccgt
      241 ctggcactgg agttgggcga tatcaacgaa cgtgatgctt caaacttcat ggtctacagc
      301 tttgcctgct tctttattgg caagtttata gccaacatcc ttatga
//
LOCUS       DADMFL010000382          345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.390, whole genome shotgun sequence.
ACCESSION   DADMFL010000382 DADMFL010000000
VERSION     DADMFL010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.390"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..318)
                     /locus_tag="JNZ27_004429"
     CDS             complement(<1..318)
                     /locus_tag="JNZ27_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8666451.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEF
                     DRLW"
BASE COUNT           76 a          102 c           85 g           82 t
ORIGIN      
        1 ccagaggcgg tcgaactccg ccttcgcaaa ataagcggaa gcctggcgaa ggatatcgtt
       61 actgcggcgc agttcacgat tttcacgctc cagctctttc agacgctgac gttcagcggt
      121 ggtgagccct ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc agacacgcag
      181 agtctccggc gtacagccaa tctttggagc aatggaacaa attgtcgccc attgtgagtc
      241 atattcgccc tgactttcca gaaccatacg gactgcccgt tgacggactt cgggggaaaa
      301 acgagtattt ttagtcatcc tgtttacctc tttctcagga agttt
//
LOCUS       DADMFL010000383          339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.391, whole genome shotgun sequence.
ACCESSION   DADMFL010000383 DADMFL010000000
VERSION     DADMFL010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.391"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>339
                     /locus_tag="JNZ27_004430"
     CDS             <1..>339
                     /locus_tag="JNZ27_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666452.1"
                     /translation="QLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQ
                     LITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLA
                     AYAGLAPVTR"
BASE COUNT           61 a          119 c           91 g           68 t
ORIGIN      
        1 cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg
       61 cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac
      121 gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag
      181 aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg
      241 tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac
      301 atctcgctgc ttatgctggc cttgcgccgg taactcggc
//
LOCUS       DADMFL010000384          332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.392, whole genome shotgun sequence.
ACCESSION   DADMFL010000384 DADMFL010000000
VERSION     DADMFL010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.392"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>332
                     /locus_tag="JNZ27_004431"
     CDS             <1..>332
                     /locus_tag="JNZ27_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666453.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGA"
BASE COUNT           99 a           80 c           92 g           61 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ct
//
LOCUS       DADMFL010000385          328 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.393, whole genome shotgun sequence.
ACCESSION   DADMFL010000385 DADMFL010000000
VERSION     DADMFL010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.393"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            1..328
                     /locus_tag="JNZ27_004432"
                     /pseudo
     CDS             1..328
                     /locus_tag="JNZ27_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           99 a           84 c           78 g           67 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtg
//
LOCUS       DADMFL010000386          328 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.394, whole genome shotgun sequence.
ACCESSION   DADMFL010000386 DADMFL010000000
VERSION     DADMFL010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.394"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(1..328)
                     /locus_tag="JNZ27_004433"
                     /pseudo
     CDS             complement(1..328)
                     /locus_tag="JNZ27_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           96 a           80 c           52 g          100 t
ORIGIN      
        1 tcaggctgta cgcttcccca tatgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaaca
//
LOCUS       DADMFL010000387          303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.395, whole genome shotgun sequence.
ACCESSION   DADMFL010000387 DADMFL010000000
VERSION     DADMFL010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.395"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(<1..278)
                     /locus_tag="JNZ27_004434"
     CDS             complement(<1..278)
                     /locus_tag="JNZ27_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666454.1"
                     /translation="MIVLILVFRLVIGEQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAM"
BASE COUNT           74 a           79 c           70 g           80 t
ORIGIN      
        1 ttcatggcgg cagcaagttc agaggcagga acaacctgtt ctccggcggc cacagcagta
       61 agacttcctt cctggtattg cttacgccag agaaataact ggctggctgc tacaccatgt
      121 tgccgggcaa cgagggagac cgtcatcccc ggttcaaagc tctgctgaac aattgcgatc
      181 ttttcctgtg tggtacgccg tctgcgtttc tccggcccta agacatcaat catctgttct
      241 ccaatgacta gtctaaaaac tagtattaag actatcactt aaataagtga tactggttgt
      301 ctg
//
LOCUS       DADMFL010000388          297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.396, whole genome shotgun sequence.
ACCESSION   DADMFL010000388 DADMFL010000000
VERSION     DADMFL010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.396"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>297
                     /locus_tag="JNZ27_004435"
     CDS             <1..>297
                     /locus_tag="JNZ27_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY8666455.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKK"
BASE COUNT          119 a           35 c           53 g           90 t
ORIGIN      
        1 aaaactgatg ttccaatatt taacactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa ggattccgag agttttgtta taacaaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagtacaga taacttcaaa aaaataaaac tcagagacat atctttggag
      241 gatgcgatta aagcatctaa ttatgaagag attaacaata aagtgactga taaaaaa
//
LOCUS       DADMFL010000389          289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.397, whole genome shotgun sequence.
ACCESSION   DADMFL010000389 DADMFL010000000
VERSION     DADMFL010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.397"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..>289
                     /locus_tag="JNZ27_004436"
     CDS             <1..>289
                     /locus_tag="JNZ27_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY8666456.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DADMFL010000390          285 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.398, whole genome shotgun sequence.
ACCESSION   DADMFL010000390 DADMFL010000000
VERSION     DADMFL010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.398"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            150..>285
                     /locus_tag="JNZ27_004437"
     CDS             150..>285
                     /locus_tag="JNZ27_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8666457.1"
                     /translation="MLHEEKLARHQRKQAMYTRMVAFPAVKMFEEYDFTFATGAPQKQ
                     L"
BASE COUNT           81 a           70 c           75 g           59 t
ORIGIN      
        1 acggtggtcg gtttacgctt accgcaggac aggtggtaac tgaaaaattg ctgaggcgac
       61 tggaaacaga agggcaagat cgattccgac agcagtgatg gtcagctacc tcatcgataa
      121 ctttgcaaca gtgcccttct tgaagcatca tgcttcatga agaaaaactg gcacgtcatc
      181 aacgtaaaca ggcgatgtat acccgaatgg tagccttccc ggcggtgaaa atgttcgaag
      241 agtatgactt cacgttcgcc accggagcac cgcagaagca gctcc
//
LOCUS       DADMFL010000391          281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.399, whole genome shotgun sequence.
ACCESSION   DADMFL010000391 DADMFL010000000
VERSION     DADMFL010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.399"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            27..103
                     /locus_tag="JNZ27_004438"
     tRNA            27..103
                     /locus_tag="JNZ27_004438"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61..63,aa:Ile,seq:gat)
     gene            146..221
                     /locus_tag="JNZ27_004439"
     tRNA            146..221
                     /locus_tag="JNZ27_004439"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:179..181,aa:Ala,seq:tgc)
BASE COUNT           76 a           62 c           74 g           69 t
ORIGIN      
        1 atgaaaatga gcagtaaaac ctctacaggc ttgtagctca ggtggttaga gcgcacccct
       61 gataagggtg aggtcggtgg ttcaagtcca ctcaggccta ccaaatttgc acggcaaatt
      121 tgaagaggtt ttaactacat gttatggggc tatagctcag ctgggagagc gcctgctttg
      181 cacgcaggag gtctgcggtt cgatcccgca tagctccacc atctctgtag tgattaaata
      241 aaaaatactt cagagtgtac ctgcaaaggt tcactgcgaa g
//
LOCUS       DADMFL010000392          279 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.denovo.400, whole genome shotgun sequence.
ACCESSION   DADMFL010000392 DADMFL010000000
VERSION     DADMFL010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.denovo.400"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            12..>279
                     /locus_tag="JNZ27_004440"
     CDS             12..>279
                     /locus_tag="JNZ27_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8666458.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVM"
BASE COUNT           90 a           65 c           65 g           59 t
ORIGIN      
        1 cgcaagaaaa aatattacgc attattcaca ccaaaggtgt gtcattgcct gatgatcgcg
       61 agtcagtctt ctggctgaat attaaaaata ttccgccatc agcgagcaac aaagcgacga
      121 actcgctgga aatagcagta aaaacgcgta tcaagctgtt ctggcgacca gcaaatatcc
      181 gccttattcc cgaagatgct gcgccgaaag tgaaatggcg ccgggaaggg cgaaatctga
      241 tagcagagaa tcccaaccca attcatatca gtgtgatgg
//
LOCUS       DADMFL010000393        17424 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.078, whole genome shotgun sequence.
ACCESSION   DADMFL010000393 DADMFL010000000
VERSION     DADMFL010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.078"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="JNZ27_004441"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="JNZ27_004441"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8666459.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="JNZ27_004442"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="JNZ27_004442"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8666460.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="JNZ27_004443"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="JNZ27_004443"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8666461.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="JNZ27_004444"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="JNZ27_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8666462.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RTRGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIHIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNZ27_004445"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNZ27_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8666463.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMMRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNZ27_004446"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNZ27_004446"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8666464.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="JNZ27_004447"
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="JNZ27_004447"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8666465.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            11316..12050
                     /locus_tag="JNZ27_004448"
     CDS             11316..12050
                     /locus_tag="JNZ27_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8666466.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="JNZ27_004449"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="JNZ27_004449"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8666467.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="JNZ27_004450"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="JNZ27_004450"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8666468.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14023
                     /gene="blaEC"
                     /locus_tag="JNZ27_004451"
                     /pseudo
     CDS             12889..14023
                     /gene="blaEC"
                     /locus_tag="JNZ27_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            14112..14645
                     /locus_tag="JNZ27_004452"
     CDS             14112..14645
                     /locus_tag="JNZ27_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8666469.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVMNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14642..14871)
                     /locus_tag="JNZ27_004453"
                     /pseudo
     CDS             complement(14642..14871)
                     /locus_tag="JNZ27_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(15047..15193)
                     /gene="ecnB"
                     /locus_tag="JNZ27_004454"
     CDS             complement(15047..15193)
                     /gene="ecnB"
                     /locus_tag="JNZ27_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8666470.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15304..15429)
                     /gene="ecnA"
                     /locus_tag="JNZ27_004455"
     CDS             complement(15304..15429)
                     /gene="ecnA"
                     /locus_tag="JNZ27_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8666471.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15481..16047)
                     /gene="efp"
                     /locus_tag="JNZ27_004456"
     CDS             complement(15481..16047)
                     /gene="efp"
                     /locus_tag="JNZ27_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8666472.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16089..17117
                     /gene="epmB"
                     /locus_tag="JNZ27_004457"
     CDS             16089..17117
                     /gene="epmB"
                     /locus_tag="JNZ27_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8666473.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4343 a         4568 c         4483 g         4030 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtacgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg ctttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 acattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgagca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acggcatccc catcgcattc aggcgcatca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga taccttgatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta cccccccctt gatatcaatg agaaaacgct
    13681 tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg
    13741 cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta acggcagtga
    13801 caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt
    13861 acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt
    13921 tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccaga
    13981 gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca tcgggtccga
    14041 attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta
    14101 aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg
    14161 ttgcctgtag ttctcctacg ccgccgcgtg gcgtgaccgt aatgaataat ttcgacgcca
    14221 aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac
    14281 tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat gtcattaata
    14341 aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg tactttaccg
    14401 gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata
    14461 acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact
    14521 acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag gagatgctgg
    14581 cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag cagcccggta
    14641 gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc agactcgcca
    14701 gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg
    14761 cgccaatacc cgtccacacg gcatcaaacg gctaaagccg tgggtatatt tcaggccaac
    14821 ggcccatacc acttccagca gaccagcaat aactaagata atccaggaca tatcaggctc
    14881 cggaaatttg gggccgtccc cggtgaaaag aggcgtttgc aggtcgtcct gcaaagctga
    14941 tggttgaacg gatattttgc caggtggcag gggaatttca actattacct ttatgacgat
    15001 cgcagaaaaa acaaacggca cgacactgct gtcgtaccgt tattgcttat tgctgcgctt
    15061 tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg
    15121 tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg
    15181 tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat
    15241 taataataga caacatcgcc aaaaatgatg gaaatctgat gatttttcgg aagagaagaa
    15301 aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt cgccgaaacc
    15361 gcaagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg
    15421 tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca
    15481 ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat
    15541 ttgtacgaac agcggaactt taaccacagc gccagtagac agggtagccg gtttgccgcc
    15601 agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa
    15661 gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg
    15721 atccagcagc catttagcgt tgtcgccaat tgcttttgca tcagcagaca gctgctcgaa
    15781 agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat
    15841 atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt
    15901 accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac
    15961 gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg
    16021 aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa
    16081 gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc
    16141 aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg
    16201 acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct
    16261 catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcgt caggtactta
    16321 cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccactg gaagaacagc
    16381 acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag
    16441 gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat gccgaaaatc
    16501 agggcaacaa gcgtaactgg cagactgcac ttgagtatgt tgctgcgcat ccggaactgg
    16561 acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt
    16621 tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac agccgtctgc
    16681 cgattgtgat cccggcacgt atcaccgagg cgctggttga acgctttgcc cgttctacgc
    16741 tgcaaatctt gctggtgaat cacatcaatc atgccaatga ggtagatgaa acgttccgtc
    16801 aggcgatggc taaattgcgc cgtgtcggtg tcaccctgct taaccagagc gttctgttac
    16861 gtggtgtgaa cgataacgca caaacgttgg caaacctgag taatgcgttg ttcgatgccg
    16921 gcgtgatgcc gtattacctg catgtgctag ataaagtaca gggcgcggcg cattttatgg
    16981 tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacattggtg tcgggatatc
    17041 tggtgccgaa actggcgcga gaaattggcg gcgaacccag caaaacgccg ctggatctcc
    17101 agctacgcca gcagtaactg attaaacagg gaagttaaac attaaaattg cctgatgcgc
    17161 tacgcttatc aggcctacat cgtgcattga aattgaatgg atttgcaatg aactgtaggc
    17221 cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc
    17281 attaaactga gaaatttaat ctttgcacgt tgcataacct ttaattattc atatgcattg
    17341 ctgttgttac tttttatata gctaacacca ataattaacc tttcttttcg gtaatgactc
    17401 caacttactg atagtgtttt atgt
//
LOCUS       DADMFL010000394         8793 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.148, whole genome shotgun sequence.
ACCESSION   DADMFL010000394 DADMFL010000000
VERSION     DADMFL010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.148"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            264..923
                     /locus_tag="JNZ27_004458"
     CDS             264..923
                     /locus_tag="JNZ27_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8666474.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JNZ27_004459"
     CDS             complement(1124..1501)
                     /locus_tag="JNZ27_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8666475.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="JNZ27_004460"
     CDS             complement(1568..4534)
                     /locus_tag="JNZ27_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY8666476.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="JNZ27_004461"
     CDS             complement(4537..5097)
                     /locus_tag="JNZ27_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8666477.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="JNZ27_004462"
     CDS             complement(5223..5573)
                     /locus_tag="JNZ27_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY8666478.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNZ27_004463"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNZ27_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY8666479.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="JNZ27_004464"
     CDS             6999..7829
                     /locus_tag="JNZ27_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY8666480.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8733
                     /gene="aadA1"
                     /locus_tag="JNZ27_004465"
     CDS             7942..8733
                     /gene="aadA1"
                     /locus_tag="JNZ27_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8666481.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2001 a         2357 c         2482 g         1953 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8641 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     8701 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8761 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
LOCUS       DADMFL010000395         8790 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.149, whole genome shotgun sequence.
ACCESSION   DADMFL010000395 DADMFL010000000
VERSION     DADMFL010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.149"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            264..923
                     /locus_tag="JNZ27_004466"
     CDS             264..923
                     /locus_tag="JNZ27_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY8666482.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JNZ27_004467"
     CDS             complement(1124..1501)
                     /locus_tag="JNZ27_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8666483.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="JNZ27_004468"
     CDS             complement(1568..4534)
                     /locus_tag="JNZ27_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY8666484.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="JNZ27_004469"
     CDS             complement(4537..5097)
                     /locus_tag="JNZ27_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8666485.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="JNZ27_004470"
     CDS             complement(5223..5573)
                     /locus_tag="JNZ27_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY8666486.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNZ27_004471"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="JNZ27_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY8666487.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="JNZ27_004472"
     CDS             6999..7829
                     /locus_tag="JNZ27_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HAY8666488.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8730
                     /gene="aadA1"
                     /locus_tag="JNZ27_004473"
     CDS             7942..8730
                     /gene="aadA1"
                     /locus_tag="JNZ27_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8666489.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1998 a         2357 c         2482 g         1953 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
     8641 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
     8701 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     8761 ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DADMFL010000396         8008 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.158, whole genome shotgun sequence.
ACCESSION   DADMFL010000396 DADMFL010000000
VERSION     DADMFL010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.158"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            complement(102..1559)
                     /gene="pepD"
                     /locus_tag="JNZ27_004474"
     CDS             complement(102..1559)
                     /gene="pepD"
                     /locus_tag="JNZ27_004474"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8666490.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            1821..2279
                     /gene="gpt"
                     /locus_tag="JNZ27_004475"
     CDS             1821..2279
                     /gene="gpt"
                     /locus_tag="JNZ27_004475"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8666491.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2371..3615
                     /gene="frsA"
                     /locus_tag="JNZ27_004476"
     CDS             2371..3615
                     /gene="frsA"
                     /locus_tag="JNZ27_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8666492.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3673..4074
                     /locus_tag="JNZ27_004477"
     CDS             3673..4074
                     /locus_tag="JNZ27_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY8666493.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4113..5168)
                     /gene="phoE"
                     /locus_tag="JNZ27_004478"
     CDS             complement(4113..5168)
                     /gene="phoE"
                     /locus_tag="JNZ27_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY8666494.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            5456..6559
                     /gene="proB"
                     /locus_tag="JNZ27_004479"
     CDS             5456..6559
                     /gene="proB"
                     /locus_tag="JNZ27_004479"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY8666495.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6571..7824
                     /gene="proA"
                     /locus_tag="JNZ27_004480"
     CDS             6571..7824
                     /gene="proA"
                     /locus_tag="JNZ27_004480"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY8666496.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         1945 a         1994 c         2081 g         1988 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccag gataatgaag gatgaaactg
       61 ccggaaggcg attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct
      121 ttcagcagtt cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca
      181 tccggagagt gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac
      241 ggttttttga acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg
      301 ttgaacaggc gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc
      361 tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa
      421 tccagcatgc tcaccacgta gtctttaccg ctgtcgatca gtgaacggat caggcagtga
      481 atttctacgt tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca
      541 ccttttgcca cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata
      601 aaggtatcgc gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac
      661 aaggccagat ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc
      721 accagagatt tcagtgcgtc gactttatca gctgtgacag caatggtcgc aaaggcttca
      781 cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct
      841 tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg
      901 atttcaccgc cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa
      961 ccagctggaa ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca
     1021 caacccatgt agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc
     1081 aaccagttgc tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc
     1141 agcacttcca gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc
     1201 ataccaatgc cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca
     1261 atataaggct ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc
     1321 accatatcga ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca
     1381 ggtttacgaa tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt
     1441 gcccaaccaa caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa
     1501 cagattttgg caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg
     1561 ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa
     1621 tgttgtcaac acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt
     1681 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     1741 cagatatcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     1801 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     1861 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     1921 ccgtaagccg tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc
     1981 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     2041 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     2101 gtggtactgc tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     2161 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     2221 ggatcgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     2281 cttttcaacg cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag
     2341 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
     2401 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
     2461 gcacaaccgc ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg
     2521 attaaccgtc tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag
     2581 gcacgtattg tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt
     2641 ggctaccgtg gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa
     2701 aaagcctgta cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg
     2761 ttgtacaaca ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag
     2821 gctttatcaa accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag
     2881 atggagttta ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc
     2941 gatggcccgt tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat
     3001 tacagcctgt atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg
     3061 ccgtcggtgg gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag
     3121 cacgtcttaa aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt
     3181 ggtttccgtt tcggcgctaa cgttgccgtg cgtttggcat accttgaatc gccgcgtctg
     3241 aaagcggttg cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag
     3301 caacaggtgc cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct
     3361 tccgacgaag cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg
     3421 gggcgtcgct gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg
     3481 gaagaggact cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca
     3541 tttaacccgg tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa
     3601 aaacgcttgt gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa
     3661 caggagatag caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt
     3721 accgcactag gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat
     3781 tgtctggctg tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg
     3841 tggatggagc tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc
     3901 gataaagcag gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg
     3961 gagcacaccc tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg
     4021 aagctggaac cggcggatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa
     4081 tgtgccggat gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg
     4141 acagtatcat cattattaat attcagctta ttatcgctat ccagtttgtt gattttataa
     4201 tcaacaaacg ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg
     4261 accagatctt catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc
     4321 gatggacgca gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta
     4381 ttggcaaagc cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga
     4441 taaatattat tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg
     4501 ccacggcttt gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg
     4561 gcgaaatcgc tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc
     4621 tttttaacgt cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg
     4681 ataacgccga agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata
     4741 aagttgtcgg tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct
     4801 tccacgtcat acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat
     4861 ttcaacccgg caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt
     4921 ccggcaaact ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca
     4981 cctttaaaac caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata
     5041 taatgcatgg ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt
     5101 tctgcagcct gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc
     5161 tttttcattt tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt
     5221 gaaaacagga aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc
     5281 tgaaaatgtg gtaatttatt aaatctgtaa taaaagcgta aacaactgcc gctacgcttg
     5341 ctgatcccgc gcaacaaaac gccatgcttt gctcgcagat ggttggcaac cgacgacagt
     5401 cctgctaaaa cgttcgtttg atatcatttt tcctaaaatt gaatggcaga gaatcatgag
     5461 tgacagccag acgctggtgg taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg
     5521 tctgaaccgt gcccatatcg ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg
     5581 gcatcggatt gttattgtga cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta
     5641 cccggaactg ccagcgacta tcgcctcgaa acaactgctg gcggcggtag ggcagagtcg
     5701 actgattcaa ctgtgggaac agctgttttc gatttatggc attcacgtcg ggcaaatgct
     5761 gctgacccgt gctgatatgg aagaccgtga acgcttcctg aacgcccgcg acaccctgcg
     5821 tgcgttgctc gatcacaata tcgttccggt aatcaatgag aacgatgctg tcgctacggc
     5881 agagattaag gtcggcgata acgataacct ttctgcgctg gcggcgattc tggcgggtgc
     5941 cgataaactg ttgctgctga ccgatcaaaa aggtttgtat accgctgatc cgcgcagcaa
     6001 tccgcaggca gaactgatta aagatgttta cggcattgat gacgcactgc gcgcgattgc
     6061 cggtgacagc gtttcaggcc tcggaactgg cggcatgagt actaaattgc aggccgctga
     6121 cgtggcttgc cgtgcgggta tcgacaccat tattgccgcg ggcagcaagc cgggcgttat
     6181 tggtgatgtg atggaaggca tttccgtcgg tacgctgttc catgcccagg cgactccgct
     6241 tgaaaaccgt aaacgctgga ttttcggtgc gccgccggcg ggtgaaatca cggtagatga
     6301 aggggcaact gccgccattc tggaacgcgg cagctccctg ttgccgaaag gcattaaaag
     6361 cgtgactggc aatttctcgc gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga
     6421 tatcgcccac ggtgtcagtc gttacaacag cgatgcatta cgccgtattg ccggacacca
     6481 ctcgcaagaa attgatgcaa tactgggata tgaatacggc ccggttgccg ttcaccgtga
     6541 tgacatgatt acccgttaag gagctggccg atgctggaac aaatgggcat tgccgcgaag
     6601 caagcctcgt ataaattagc gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa
     6661 atcgccgatg aactggaagc acaaagcgaa atcatcctca acgctaacgc ccaggatgtt
     6721 gctgacgcgc gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc
     6781 gcacggctga aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg
     6841 gggcaggtaa tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta
     6901 ccgctggggg ttattggcgt gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct
     6961 tcgctgtgcc tgaaaaccgg taatgcggtg atcctgcgtg gtggtaaaga aacctgtcgc
     7021 actaacgcgg caacggtggc ggtgattcag gacgccctga aatcctgtgg cttaccggcg
     7081 ggtgccgtgc aggcgattga taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg
     7141 gataaataca tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt
     7201 gaacagtcga caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat
     7261 gaaagcgcag agatcgctga agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg
     7321 agcacatgta atacggttga aacgttgctg gtgaataaaa acatcgccga tagcttcctg
     7381 cccgcattaa gcaaacaaat ggcggaaagc ggcgtgacat tacacgcaga tgcagctgcg
     7441 ctgacacagt tgcaggcagg ccctgcgaag gtggtggcgg ttaaagccga agagtatgac
     7501 gatgagtttc tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc
     7561 gcccatattc gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc
     7621 aacgcccagc gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg
     7681 cgttttaccg acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa
     7741 ctccacgcgc gtggcccaat ggggctggaa gcactgacca cttacaagtg gatcggcatt
     7801 ggtgattaca ccattcgtgc gtaaataaaa ccgggtgatg caaaagtagc catttgattc
     7861 acaaggccat tgacgcatcg cccggttagt tttaaccttg tccaccgtga ttcacgttcg
     7921 tgaacatgtc ctttcagggc cgatatagct cagttggtag agcagcgcat tcgtaatgcg
     7981 aaggtcgtag gttcgactcc tattatcg
//
LOCUS       DADMFL010000397         5817 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.186, whole genome shotgun sequence.
ACCESSION   DADMFL010000397 DADMFL010000000
VERSION     DADMFL010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.186"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="JNZ27_004481"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="JNZ27_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY8666497.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1451..2254
                     /locus_tag="JNZ27_004482"
                     /note="aph(3'')-Ib"
     CDS             1451..2254
                     /locus_tag="JNZ27_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY8666498.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2254..3090
                     /locus_tag="JNZ27_004483"
     CDS             2254..3090
                     /locus_tag="JNZ27_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY8666499.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3062..3601)
                     /locus_tag="JNZ27_004484"
     CDS             complement(3062..3601)
                     /locus_tag="JNZ27_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8666500.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(3645..4034)
                     /locus_tag="JNZ27_004485"
     CDS             complement(3645..4034)
                     /locus_tag="JNZ27_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8666501.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4204..4692
                     /locus_tag="JNZ27_004486"
     CDS             4204..4692
                     /locus_tag="JNZ27_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HAY8666502.1"
                     /translation="MFCCYNTVHTGDTVTFMAWHTVQETLKLTGKSRSQLYRDMAKGL
                     VSYRTGKNDRREFETSELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKL
                     DALQQTVTLMLEDKMAREAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSG
                     SE"
BASE COUNT         1431 a         1521 c         1516 g         1349 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa
     1441 ggaacctcca ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc
     1501 tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc
     1561 gaaaatcgca cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg
     1621 gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg
     1681 tgcatgcttg gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga
     1741 tttgctcaaa gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt
     1801 tgatcaatgt ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt
     1861 gtcccgcaat gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct
     1921 cgatcttttg gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga
     1981 tatggttgtt tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct
     2041 tcaatgcacg ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt
     2101 ggcactcatg attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc
     2161 cttcgctgtc ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt
     2221 ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct
     2281 cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa
     2341 gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt
     2401 gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg
     2461 ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct
     2521 cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg
     2581 aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc
     2641 tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac
     2701 gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta
     2761 catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat
     2821 cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct
     2881 gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct
     2941 cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt
     3001 tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg
     3061 atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga
     3121 acacatcaat ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct
     3181 tcccgcaaac gaccccaaac aggaaacgca gctgaaacgg gaagctcaac acccactgac
     3241 gcatgggttg ttcaggcagt acttcatcaa ccagcaaggc ggcactttcg gccatccgcc
     3301 gcgccccaca gctcgggcag aaaccgcgac gcttacagct gaaagcgacc aggtgctcgg
     3361 cgtggcaaga ctcgcagcga acccgtagaa agccatgctc cagccgcccg cattggagaa
     3421 attcttcaaa ttcccgttgc acatagcccg gcaattcctt tccctgctct gccataagcg
     3481 cagcgaatgc cgggtaatac tcgtcaacga tctgatagag aagggtttgc tcgggtcggt
     3541 ggctctggta acgaccagta tcccgatccc ggctggccgt cctggccgcc acatgaggca
     3601 tgttccgcgt ccttgcaata ctgtgtttac atacagtcta tcgcttagcg gaaagttctt
     3661 ttaccctcag ccgaaatgcc tgccgttgct agacattgcc agccagtgcc cgtcactccc
     3721 actcttacat tgttcgcagt atctaggtac atgttattgc gtgcaccttc atgatgtata
     3781 atgttattaa tgacagcaga aagatcgaca gaattaatat tcttcgtacc tgggacagtt
     3841 atcgggtgtc ggccagtgat gagaccaaag gatgcagtat aagatgaacc actactatta
     3901 tcatggccac cattaccacc accgttaaaa ctacctcccg aacttccaga gccgctatga
     3961 ccatcattgt aggggtcatt tggtcccatg tgaccaaaac caccgacagt accgctattt
     4021 aactctgtac tcataaaatt atcctctttg acttttaaaa acagcaaaaa ctgtatttat
     4081 aacaatgtgt ttatatacag tattcgaaat actacgcccg tcaaaaccac accgtcaaca
     4141 ggttttttgt gtatttttca cgcctcacgg gcagcctcag accttttgtg ttaaaacagt
     4201 aatatgttct gctgttacaa tacggtacac acaggagaca ctgtcacttt tatggcatgg
     4261 cacacagttc aagagacact gaaattaaca ggaaagtcgc gctcgcaatt atatcgtgac
     4321 atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg atcgccggga gttcgagacc
     4381 agtgaactga ttcgggccta tggcgagctg cgcacttctg agacaccaaa aaggcacaag
     4441 gagggacact ccaaatctgt cgttagccag gcggaaaatg ctcagctaga cgtcattcag
     4501 cagaaactgg acgctctgca gcagactgtt accctaatgc ttgaagacaa gatggcgcgg
     4561 gaagcagaaa accgcctgcg aaatgaggag agggaacaac tacaggccga aattagccgt
     4621 ctggctgacg cgctggcaca ggaaaaaaag cggggattct ggtcgcggct gtttcgctcc
     4681 ggttcagagt aacagtgcag gtgactctga tttaccataa catctgttat cagcaccgcc
     4741 tgagaggccc tcagggcgtt tctgacggga aaccggtaaa acaggcaaca aaccacccga
     4801 aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcacaagtg
     4861 catagcgcgg aaattcacac cattcagccg gggtaagtcc tgtttccggc gttcttccca
     4921 tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag
     4981 ccggaaaacc cgctgtcagc acgttcagga agggggtgat gtacaaatgg cacagaggat
     5041 gaaagtgacg tatgagctga aggaatgatg taaaagtgat gtaaaagtga tgtataagtg
     5101 atgtatgaac tgaaggagcg atgtaattct gtagtcagga agaataaccc gttcatgcag
     5161 gcatgaacga tacagtgagg aacgcttttt ttgtcacgat ctccccttaa cctgcgtaaa
     5221 tcacccgcca tggttgatta tttttcaacc tcaaaatggt catacaccta acgcagccgc
     5281 gattttcatc gcaactgcat gaattaaaag aaaaaaaagg aatatttcgt tttcacgagt
     5341 aatacccggg tattaaaaat ccactgtaat tacatgaaaa taaagaaata aaaggtaata
     5401 ccggagtatt accacgtacg cgggcaagcg aaaaagctat aacgcttccc ggccgacagg
     5461 cgacccaaaa tccacagggg catgcaggtt ctcttcagta acctgcgcca ttaaatcaaa
     5521 aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat catattcaga
     5581 tggcatgggg tgatatccag tagctgagag gttgttagcg ggtttcgggg cgcagccctg
     5641 aaccagtcat gtagcgctag cggagtgtat actggcttac tatgttgcca cgattgcgaa
     5701 aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg tcagcagaat
     5761 atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc ggtcgtt
//
LOCUS       DADMFL010000398         5720 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.189, whole genome shotgun sequence.
ACCESSION   DADMFL010000398 DADMFL010000000
VERSION     DADMFL010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.189"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            <1..80
                     /locus_tag="JNZ27_004487"
     CDS             <1..80
                     /locus_tag="JNZ27_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8666503.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            77..340
                     /locus_tag="JNZ27_004488"
     CDS             77..340
                     /locus_tag="JNZ27_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8666504.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            333..719
                     /locus_tag="JNZ27_004489"
     CDS             333..719
                     /locus_tag="JNZ27_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY8666505.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            727..1413
                     /locus_tag="JNZ27_004490"
     CDS             727..1413
                     /locus_tag="JNZ27_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8666506.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1391..2014)
                     /gene="tetR(B)"
                     /locus_tag="JNZ27_004491"
     CDS             complement(1391..2014)
                     /gene="tetR(B)"
                     /locus_tag="JNZ27_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY8666507.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2096..3301
                     /gene="tet(B)"
                     /locus_tag="JNZ27_004492"
     CDS             2096..3301
                     /gene="tet(B)"
                     /locus_tag="JNZ27_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY8666508.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3414..4007)
                     /gene="tetC"
                     /locus_tag="JNZ27_004493"
     CDS             complement(3414..4007)
                     /gene="tetC"
                     /locus_tag="JNZ27_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY8666509.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4095..4505
                     /locus_tag="JNZ27_004494"
                     /pseudo
     CDS             4095..4505
                     /locus_tag="JNZ27_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4521..>5720)
                     /locus_tag="JNZ27_004495"
     CDS             complement(4521..>5720)
                     /locus_tag="JNZ27_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY8666510.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1582 a         1057 c         1245 g         1836 t
ORIGIN      
        1 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
       61 acataaaaca ctatcaataa gttggagtca ttaccgacca ctattaagtc atgtagctgg
      121 ttttatttca attgaacgtt ttcaaagtct agctacagaa ggaaaaatgt tatcgctatc
      181 ttggtgggaa aacgaatacg cagttctgca atggaaaaat catgttttac atgcgaaagc
      241 tcaacaagaa gggcgagagt caatatttga tttttacaaa attagtattg ctcatattac
      301 tcgcgaatat tcatttaaaa aggacaagga taatgtttga tgttcacgtt gttttagata
      361 atcaaatagg acaattagca ttactaggaa aaacattagg taataaaggt attggattgg
      421 aagggggagg gatatttacg gttggtgatg aatgccatgc tcattttctt gttgaacaag
      481 gaaaggaagc taaaatagcg ctagagcaag ctggactgtt agtacttgcg atccggacac
      541 cattaattcg taagttaaaa caggaaaaac cgggggaact tggcgaaata gcacgagtat
      601 tggcggagaa taacattaat attttagtgc aatacagtga ccatgctaac caactgatat
      661 taataacgga caatgatagt atggctgcat ctgttacgct cccttgggca ataaagtgaa
      721 cttgcgatgg ctaatttaat acgaaaagag gttacctttg agtcctcaat agccgcgata
      781 ggggcggcta tgtctgacat ttcacgagtt aaaatactca gtgctttgat ggatgggcga
      841 gcttggacgg ccactgagct aagttctgtg gcgaatatat cagcttcaac ggcgagcagt
      901 catttatcta aattattaga ttgccagcta atcacagtag tagctcaagg caagcatcgt
      961 tattttcggc tagcaggaaa agatattgct gaattgatgg aaagtatgat ggggatctcc
     1021 ttaaaccatg gcgtacatgc caaagtttcc acgccagtgc atttacgaaa agcacgtact
     1081 tgctatgatc atttagctgg cgaagttgcc gttaagatct atgattccct ttgtcaacag
     1141 caatggatca ctgaaaatgg ttcaatgatc acattaagtg gtattcaata ttttcatgaa
     1201 atgggaattg acgttccttc caaacattca cgtaaaatct gttgtgcgtg tttagattgg
     1261 agtgaacgcc gtttccattt aggtgggtac gttggagccg cattattttc gctttatgaa
     1321 tctaaagggt ggttaactcg acatcttggt taccgtgaag ttaccatcac ggaaaaaggt
     1381 tatgctgctt ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
     1441 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
     1501 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
     1561 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
     1621 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
     1681 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
     1741 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
     1801 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
     1861 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
     1921 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
     1981 tgttaatcac tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac
     2041 tctatcattg atagagttat tttaccactc cctatcagtg atagagaaaa gtgaaatgaa
     2101 tagttcgaca aagatcgcat tggtaattac gttactcgat gccatgggga ttggccttat
     2161 catgccagtc ttgccaacgt tattacgtga atttattgct tcggaagata tcgctaacca
     2221 ctttggcgta ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc cttggcttgg
     2281 aaaaatgtct gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc
     2341 gctggattac ttattgctgg ctttttcaag tgcgctttgg atgctgtatt taggccgttt
     2401 gctttcaggg atcacaggag ctactggggc tgtcgcggca tcggtcattg ccgataccac
     2461 ctcagcttct caacgcgtga agtggttcgg ttggttaggg gcaagttttg ggcttggttt
     2521 aatagcgggg cctattattg gtggttttgc aggagagatt tcaccgcata gtcccttttt
     2581 tatcgctgcg ttgctaaata ttgtcgcttt ccttgtggtt atgttttggt tccgtgaaac
     2641 caaaaataca cgtgataata cagataccga agtaggggtt gagacgcaat cgaattcggt
     2701 atacatcact ttatttaaaa cgatgcccat tttgttgatt atttattttt cagcgcaatt
     2761 gataggccaa attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa
     2821 tagcatgatg gttggctttt cattagcggg tcttggtctt ttacactcag tattccaagc
     2881 ctttgtggca ggaagaatag ccactaaatg gggcgaaaaa acggcagtac tgctcggatt
     2941 tattgcagat agtagtgcat ttgccttttt agcgtttata tctgaaggtt ggttagtttt
     3001 ccctgtttta attttattgg ctggtggtgg gatcgcttta cctgcattac agggagtgat
     3061 gtctatccaa acaaagagtc atcagcaagg tgctttacag ggattattgg tgagccttac
     3121 caatgcaacc ggtgttattg gcccattact gtttgctgtt atttataatc attcactacc
     3181 aatttgggat ggctggattt ggattattgg tttagcgttt tactgtatta ttatcctgct
     3241 atcgatgacc ttcatgttaa cccctcaagc tcaggggagt aaacaggaga caagtgctta
     3301 gttatttcgt caccaaatga tgttattccg cgaaatataa tgaccctctt gataacccaa
     3361 gagggcattt tttacgataa agaagattta gcttcaaata aaacctatct attttattta
     3421 tctttcaagc tcaataaaaa gccgcggtaa atagcaataa attggccttt tttatcggca
     3481 agctctttta ggtttttcgc atgtattgcg atatgcataa accagccatt gagtaagttt
     3541 ttaagcacat catcatcata agctttaagt tggttctctt ggatcaattt gctgacaatg
     3601 gcgtttacct taccagtaat gtattcaagg ctaatttttt caagttcatt ccaaccaatg
     3661 ataggcatca cttcttggat agggataagg tttttattat tatcaataat ataatcaaga
     3721 taatgttcaa atatactttc taaggcagac caaccatttg ttaaatcagt ttttgttgtg
     3781 atgtaggcat caatcataat taattgctgc ttataacagg cactgagtaa ttgtttttta
     3841 tttttaaagt gatgataaaa ggcacctttg gtcaccaacg cttttcccga gatctcatct
     3901 attgaaacag cttgatagcc tttttcaaca aacaatattc gtgctgagtt aaccagtgat
     3961 tgataggtac tcttaaaatt ttcttgttga tgatttttat tttccatgat agatttaaaa
     4021 taacataccg tcagtatgtt tatggtatca tgatgatgtg gtcgtgacaa tcttaagaac
     4081 atttaggtta ttttatgtat attgaacagc attctcgcta tcaaaataaa gctaataaca
     4141 tccaattaag atatgatgat aagcagtttc atacaacggt tatcaaagat gttctattat
     4201 ggattgaaca taatttagat cagtctttac tgcttgatga tgtggcgaat aaagcgggtt
     4261 ataccaagtg gtattttcag cggctgttca aaaaagtaac aggggtcaca ctggctagct
     4321 atattcgtgc tcgtcgtttg acgaaagcgg ctgttgagtt gaggttgacg aaaaaaacta
     4381 tccttgagat cgcattaaaa tatcaatttg attcccaaca atcttttaca cgtcgattta
     4441 agtacatttt taaggttaca ccaagttatt atcggcgtaa taaattatgg gaattggagg
     4501 caatgcactg agagatcccc tcataatttc cccaaagcgt aaccatgtgt gaataaattt
     4561 tgagctagta gggttgcagc cacgagtaag tcttcccttg ttattgtgta gccagaatgc
     4621 cgcaaaactt ccatgcctaa gcgaactgtt gagagtacgt ttcgatttct gactgtgtta
     4681 gcctggaagt gcttgtccca accttgtttc tgagcatgaa cgcccgcaag ccaacatgtt
     4741 agttgaagca tcagggcgat tagcagcatg atatcaaaac gctctgagct gctcgttcgg
     4801 ctatggcgta ggcctagtcc gtaggcagga cttttcaagt ctcggaaggt ttcttcaatc
     4861 tgcattcgct tcgaatagat attaacaagt tgtttgggtg ttcgaatttc aacaggtaag
     4921 ttagttgcta gaacccatgg ctcctttgcc gacgctgagt agattttagg tgacgggtgg
     4981 tgacaatgag tccgtgtcga gcgctgattt tttcggcctt tagagcgaga tttatacaat
     5041 agaatttggc atgagattgg attgctttta gtcagcctct tatagcctaa agtctttgag
     5101 tgactagatg acatatcatg taagttgctg ataggtttcc agttttccgc tcctaggtct
     5161 gcatattgta cttttcctct tactcgactt aaccagtacc aacccagctt ctcaacggat
     5221 ttataccatg gcactttaaa gccagcatca ctgacaatga gcggtgtggt gttactcggt
     5281 agaatgctcg caaggtcggc tagaaattgg tcatgagctt tctttgaaca ttgctctgaa
     5341 agcgggaacg ctttctcata aagagtaaca gaacgaccgt gtagtgcgac tgaagctcgc
     5401 aataccataa gtcgtttttg ctcacgaata tcagaccagt caacaagtac aatgggcatc
     5461 gtattgcccg aacagataaa gctagcatgc caacggtata cagcgagtcg ctctttgtgg
     5521 aggtgacgat tacctaacaa tcggtcgatt cgtttgatgt tatgttttgt tctcgctttg
     5581 gttggcaggt tacggccaag ttcggtaaga gtgagagttt tacagtcaag taatgcgtgg
     5641 caagccaacg ttaagctgtt gagtcgtttt aagtgtaatt cggggtaatg actccaactt
     5701 actgatagtg ttttatgttc
//
LOCUS       DADMFL010000399         4297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.220, whole genome shotgun sequence.
ACCESSION   DADMFL010000399 DADMFL010000000
VERSION     DADMFL010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.220"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            560..799
                     /locus_tag="JNZ27_004496"
     CDS             560..799
                     /locus_tag="JNZ27_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY8666511.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(987..1964)
                     /gene="intI2"
                     /locus_tag="JNZ27_004497"
                     /pseudo
     CDS             complement(987..1964)
                     /gene="intI2"
                     /locus_tag="JNZ27_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2296..2769
                     /locus_tag="JNZ27_004498"
     CDS             2296..2769
                     /locus_tag="JNZ27_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY8666512.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2864..3388
                     /gene="sat2"
                     /locus_tag="JNZ27_004499"
     CDS             2864..3388
                     /gene="sat2"
                     /locus_tag="JNZ27_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY8666513.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3446..4237
                     /gene="aadA1"
                     /locus_tag="JNZ27_004500"
     CDS             3446..4237
                     /gene="aadA1"
                     /locus_tag="JNZ27_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8666514.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1207 a          945 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagccc tgtgggcgga
       61 caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat ttagggaaga
      121 gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa ttaaaaatga
      181 caaaatagtt tggaactaga tttcacttat ctggttaact aatctagcta acctgccgct
      241 cagcttagta cgatcctata tccaactgat cataatcctt tcatcagtat cccaaattaa
      301 atccccctcc tagactgcct caccaacact ttacagtgat cccgtatttc cggacaggtg
      361 attgattttt ttcgcttgta caggtgttaa gccacagtta tcttgatgtg gacattatgc
      421 attgtaatag cccattaaca tgcgctaaga gcataaatta atatacagta acaagcatta
      481 cgaggtgcta atgtttggaa agcagcctct tccaactaca ataataagtt aactatcctc
      541 cacccacaaa taattacaaa tgaccccttt ccgctataac tccgatctca ccagcggctc
      601 gctacaaacc cgagagtgtc gcattattac tggcttgtta ctccaagagc ttgatgaagc
      661 tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag aaacgcacgc aatctacggt
      721 aagacgtatt tcttcagcgc ttagaaaacg cctagaacac ctaagctctg acttttgggc
      781 ttttgcgttt ttatgttagg cattgtctgg caactggagg aagtgtaatg accatgaatg
      841 gtcactgtga ttgacagtat cttgcttaaa cttatctgaa caatggtaga actggctcat
      901 atttatcccc tctatgattg tttatctttt tcaccataaa aagtatcagg ggctactcag
      961 ggctacataa agatatttat ctacttttac tgattgataa gtagcatcag tccatccgca
     1021 ggactggtgg tgccggcaaa atgctgaccc aacacatgcg tatagatttg cgtggtctta
     1081 acatcgttat gccctaagag ttcttgcaca gtgcgaatat cacgccccgc ttgtaataga
     1141 tgcgtagcaa acgagtgacg aaatgtatga caagtgacac gcttgctaac gatgcctgct
     1201 ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt catgcagatg atggcggcat
     1261 aatttgccgt tatacgggtg gttgcagagc gtgctggagg gaaagacaaa catccacgcc
     1321 gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa aaggcagcga tggccctacg
     1381 ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct caatgagtta ttttattgct
     1441 gggattaggc gcgtgggcag taggctgttt ctgcttttcc cacccttacc gtcatgcaca
     1501 gtgatgcagc cattatcaaa atcaaaatct ttaacccgca aacgcaagca ttcattaatg
     1561 cgcaaacctg caccatacag cagcgtaaaa ataacttggt tgcgagtatc cataacctgc
     1621 aaaatgcgtt gcacttcatt tgcagagata acagagggta gccgtctagg cttgcttgca
     1681 gggatataat caatatcgcc caacggctgt tgtaaaaacc tgttgtacaa aaaagctagg
     1741 gcatttaaag cgattttctg cgtgtttatg gctacatgtc tgctgtttgc taagctggat
     1801 aaaaacagcc tgacctcttc actgcccatg gtctgaggat gacgtttttt gtgaaacaga
     1861 ataaaacgct taatccagtg caggtaagtt ttttcagttt tcagcgcata acctttttgt
     1921 cgcatatccg tgcgtataga atttaaaaat ggactgttag acataaaacg ctccttgtct
     1981 tgcaactgtc tgcctataca gcctattcta gctgggattt aaaaaagtgc ctgtttttta
     2041 cgcctagaga tgcttgttta ccggtagagt tttaatttaa tgctaaataa attaaaatgt
     2101 tatgagttct ttgggtgaga taatgtgcat cgtgcaagca ggatagacgg catgcacgat
     2161 ttgtaataac agagtgtctt gtatttttaa agaaagtcta tttaatacaa gtgattatat
     2221 taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata aaatgttaac
     2281 ctctgaggaa gaattgtgaa actatcacta atggtagcta tatcgaagaa tggagttatc
     2341 gggaatggcc ctgatattcc atggagtgcc aaaggtgaac agctcctgtt taaagctatt
     2401 acctataacc aatggctgtt ggttggacgc aagacttttg aatcaatggg agcattaccc
     2461 aaccgaaagt atgcggtcgt aacacgttca agttttacat ctgacaatga gaacgtattg
     2521 atctttccat caattaaaga tgctttaacc aacctaaaga aaataacgga tcatgtcatt
     2581 gtttcaggtg gtggggagat atacaaaagc ctgatcgatc aagtagatac actacatata
     2641 tctacaatag acatcgagcc ggaaggtgat gtttactttc ctgaaatccc cagcaatttt
     2701 aggccagttt ttacccaaga cttcgcctct aacataaatt atagttacca aatctggcaa
     2761 aagggttaac aagtggcagc aacggattcg caaacctgtc acgccttttg taccaaaagc
     2821 cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt catatgaaga tttcggtgat
     2881 ccctgagcag gtggcggaaa cattggatgc tgagaaccat ttcattgttc gtgaagtgtt
     2941 cgatgtgcac ctatccgacc aaggctttga actatctacc agaagtgtga gcccctaccg
     3001 gaaggattac atctcggatg atgactctga tgaagactct gcttgctatg gcgcattcat
     3061 cgaccaagag cttgtcggga agattgaact caactcaaca tggaacgatc tagcctctat
     3121 cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga gtcgcgcaca gtctcatcga
     3181 atttgcgaaa aagtgggcac taagcagaca gctccttggc atacgattag agacacaaac
     3241 gaacaatgta cctgcctgca atttgtacgc aaaatgtggc tttactctcg gcggcattga
     3301 cctgttcacg tataaaacta gacctcaagt ctcgaacgaa acagcgatgt actggtactg
     3361 gttctcggga gcacaggatg acgcctaaca attcattcaa gccgacaccg cttcgcggcg
     3421 cggcttaatt caggagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     3481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     4021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     4081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     4141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta
     4201 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg
     4261 acgccgcttc gcggcgcggc ttaactcaag cgttaga
//
LOCUS       DADMFL010000400         4294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180084
            SAMEA1710188-rid11411123.guided.221, whole genome shotgun sequence.
ACCESSION   DADMFL010000400 DADMFL010000000
VERSION     DADMFL010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710188
            Sequence Read Archive: ERR230514
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941174.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941174.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:23:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,425
            Genes (coding)                    :: 4,027
            CDSs (with protein)               :: 4,027
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2 (16S, 23S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 398
            CDSs (without protein)            :: 398
            Pseudo Genes (ambiguous residues) :: 0 of 398
            Pseudo Genes (frameshifted)       :: 199 of 398
            Pseudo Genes (incomplete)         :: 102 of 398
            Pseudo Genes (internal stop)      :: 135 of 398
            Pseudo Genes (multiple problems)  :: 35 of 398
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710188-rid11411123.guided.221"
                     /strain="ERS180084"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2010"
     gene            560..799
                     /locus_tag="JNZ27_004501"
     CDS             560..799
                     /locus_tag="JNZ27_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY8666515.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(987..1964)
                     /gene="intI2"
                     /locus_tag="JNZ27_004502"
                     /pseudo
     CDS             complement(987..1964)
                     /gene="intI2"
                     /locus_tag="JNZ27_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2296..2769
                     /locus_tag="JNZ27_004503"
     CDS             2296..2769
                     /locus_tag="JNZ27_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY8666516.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2864..3388
                     /gene="sat2"
                     /locus_tag="JNZ27_004504"
     CDS             2864..3388
                     /gene="sat2"
                     /locus_tag="JNZ27_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY8666517.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3446..4234
                     /gene="aadA1"
                     /locus_tag="JNZ27_004505"
     CDS             3446..4234
                     /gene="aadA1"
                     /locus_tag="JNZ27_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY8666518.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1204 a          945 c         1000 g         1145 t
ORIGIN      
        1 gatcggtcaa ctgatcctta actgatcggc attacaccgg tcggcagccc tgtgggcgga
       61 caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat ttagggaaga
      121 gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa ttaaaaatga
      181 caaaatagtt tggaactaga tttcacttat ctggttaact aatctagcta acctgccgct
      241 cagcttagta cgatcctata tccaactgat cataatcctt tcatcagtat cccaaattaa
      301 atccccctcc tagactgcct caccaacact ttacagtgat cccgtatttc cggacaggtg
      361 attgattttt ttcgcttgta caggtgttaa gccacagtta tcttgatgtg gacattatgc
      421 attgtaatag cccattaaca tgcgctaaga gcataaatta atatacagta acaagcatta
      481 cgaggtgcta atgtttggaa agcagcctct tccaactaca ataataagtt aactatcctc
      541 cacccacaaa taattacaaa tgaccccttt ccgctataac tccgatctca ccagcggctc
      601 gctacaaacc cgagagtgtc gcattattac tggcttgtta ctccaagagc ttgatgaagc
      661 tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag aaacgcacgc aatctacggt
      721 aagacgtatt tcttcagcgc ttagaaaacg cctagaacac ctaagctctg acttttgggc
      781 ttttgcgttt ttatgttagg cattgtctgg caactggagg aagtgtaatg accatgaatg
      841 gtcactgtga ttgacagtat cttgcttaaa cttatctgaa caatggtaga actggctcat
      901 atttatcccc tctatgattg tttatctttt tcaccataaa aagtatcagg ggctactcag
      961 ggctacataa agatatttat ctacttttac tgattgataa gtagcatcag tccatccgca
     1021 ggactggtgg tgccggcaaa atgctgaccc aacacatgcg tatagatttg cgtggtctta
     1081 acatcgttat gccctaagag ttcttgcaca gtgcgaatat cacgccccgc ttgtaataga
     1141 tgcgtagcaa acgagtgacg aaatgtatga caagtgacac gcttgctaac gatgcctgct
     1201 ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt catgcagatg atggcggcat
     1261 aatttgccgt tatacgggtg gttgcagagc gtgctggagg gaaagacaaa catccacgcc
     1321 gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa aaggcagcga tggccctacg
     1381 ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct caatgagtta ttttattgct
     1441 gggattaggc gcgtgggcag taggctgttt ctgcttttcc cacccttacc gtcatgcaca
     1501 gtgatgcagc cattatcaaa atcaaaatct ttaacccgca aacgcaagca ttcattaatg
     1561 cgcaaacctg caccatacag cagcgtaaaa ataacttggt tgcgagtatc cataacctgc
     1621 aaaatgcgtt gcacttcatt tgcagagata acagagggta gccgtctagg cttgcttgca
     1681 gggatataat caatatcgcc caacggctgt tgtaaaaacc tgttgtacaa aaaagctagg
     1741 gcatttaaag cgattttctg cgtgtttatg gctacatgtc tgctgtttgc taagctggat
     1801 aaaaacagcc tgacctcttc actgcccatg gtctgaggat gacgtttttt gtgaaacaga
     1861 ataaaacgct taatccagtg caggtaagtt ttttcagttt tcagcgcata acctttttgt
     1921 cgcatatccg tgcgtataga atttaaaaat ggactgttag acataaaacg ctccttgtct
     1981 tgcaactgtc tgcctataca gcctattcta gctgggattt aaaaaagtgc ctgtttttta
     2041 cgcctagaga tgcttgttta ccggtagagt tttaatttaa tgctaaataa attaaaatgt
     2101 tatgagttct ttgggtgaga taatgtgcat cgtgcaagca ggatagacgg catgcacgat
     2161 ttgtaataac agagtgtctt gtatttttaa agaaagtcta tttaatacaa gtgattatat
     2221 taattaacgg taagcatcag cgggtgacaa aacgagcatg cttactaata aaatgttaac
     2281 ctctgaggaa gaattgtgaa actatcacta atggtagcta tatcgaagaa tggagttatc
     2341 gggaatggcc ctgatattcc atggagtgcc aaaggtgaac agctcctgtt taaagctatt
     2401 acctataacc aatggctgtt ggttggacgc aagacttttg aatcaatggg agcattaccc
     2461 aaccgaaagt atgcggtcgt aacacgttca agttttacat ctgacaatga gaacgtattg
     2521 atctttccat caattaaaga tgctttaacc aacctaaaga aaataacgga tcatgtcatt
     2581 gtttcaggtg gtggggagat atacaaaagc ctgatcgatc aagtagatac actacatata
     2641 tctacaatag acatcgagcc ggaaggtgat gtttactttc ctgaaatccc cagcaatttt
     2701 aggccagttt ttacccaaga cttcgcctct aacataaatt atagttacca aatctggcaa
     2761 aagggttaac aagtggcagc aacggattcg caaacctgtc acgccttttg taccaaaagc
     2821 cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt catatgaaga tttcggtgat
     2881 ccctgagcag gtggcggaaa cattggatgc tgagaaccat ttcattgttc gtgaagtgtt
     2941 cgatgtgcac ctatccgacc aaggctttga actatctacc agaagtgtga gcccctaccg
     3001 gaaggattac atctcggatg atgactctga tgaagactct gcttgctatg gcgcattcat
     3061 cgaccaagag cttgtcggga agattgaact caactcaaca tggaacgatc tagcctctat
     3121 cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga gtcgcgcaca gtctcatcga
     3181 atttgcgaaa aagtgggcac taagcagaca gctccttggc atacgattag agacacaaac
     3241 gaacaatgta cctgcctgca atttgtacgc aaaatgtggc tttactctcg gcggcattga
     3301 cctgttcacg tataaaacta gacctcaagt ctcgaacgaa acagcgatgt actggtactg
     3361 gttctcggga gcacaggatg acgcctaaca attcattcaa gccgacaccg cttcgcggcg
     3421 cggcttaatt caggagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     3481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     4021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     4081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctaggc aggcttatct
     4141 tggacaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg ttcactacgt
     4201 gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt caagccgacg
     4261 ccgcttcgcg gcgcggctta actcaagcgt taga
//
